Arabidopsis Cytochrome P450 ESTs (alphabetical)

Family Assignments

Last modified Aug. 3, 1999


Revised Aug. 3, 1999 6 more ESTs identified 26/254 = 10% are not yet identified
Currently there are 254 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 18 separate genes in 9 families are represented by these 
26 ESTs

Revised May 18, 1999 2 more ESTs identified 32/254 = 13% are not yet identified
Currently there are 254 ESTs in this table.  Because some of these ESTs are from the same 
sequence, there are no more than 22 separate genes represented by these 32 ESTs

Revised April 26, 1999 9 more ESTs identified 32/252 = 13% are not yet identified
Currently there are 252 ESTs in this table. Because some ESTs are from the same 
sequence, these 32 ESTs represent no more than 22 different sequences.

Revised April 16, 1999 2 more ESTs identified 41/252 = 16% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 15, 1999 4 more ESTs identified 43/252 = 17% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 13, 1999 3 more ESTs identified 47/252 = 19% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 6, 1999 3 more ESTs identified 50/252 = 20% are not yet identified
Currently there are 252 ESTs in this table.

Revised April 2, 1999 13 more ESTs identified 50/249 = 20% are not yet identified
Currently there are 249 ESTs in this table.

Revised Mar. 26, 1999  6 more ESTs identified 25% of ESTs are still not identified
Currently there are 247 ESTs in this table.

Revised Mar. 3, 1999  18 more ESTs identified 27% of ESTs are still not identified

Completely revised April 21, 1998 (new EST search done) Search strategy was
(P450 AND arabidopsis[LIB LIBX CLIN]) NOT (reductase OR D64052)
This last exclusion is to prevent matches to a P450-like TATA Binding Protein.
This search resulted in 217 hits, 13 were new.  My old list had 230 hits, so 26 of the old 
numbers were not found on this search.  I know that three have been deleted from 
genbank (T04452, T04714 and T13009).  I don't know why.

The assignments made below are subject to change when longer fragments become 
available.  I have tried to be as careful as possible in making assignments, but I cannot 
foretell the future.  All percents refer to protein sequence identities.

When clone names indicate the same clone has been sequenced from both ends, these 
fragments are included in one sequence (example: T04186 and T04714) 
T7 at the end of a clone name indicates the T7 primer was used for sequencing.
XP at the end of a clone name means the M13 reverse primer was used.

Comparisons between percent identities are not meaningful unless the fragments cover the 
same region of the P450 sequence.  The C-terminal is more highly conserved than the N-
terminal and this is more conserved than the middle of the sequences.  30% identity in the 
middle may be significant while 50% might be low at the C-terminal.

163 fragments were returned from a search of the EST database with the boolean
expression (P450 AND Arabidopsis[LIB]) NOT reductase on 7/9/96.
Another search was done May 19, 1997 and the new ESTs are included.  The most recent 
search was April 14, 1998 which only found 13 new hits.

A large number of hits to P450 as a string search were to a sequence D64052 from tobacco.  
This has been published as a P450-like TBP sequence, however, the sequence has a blastn 
score of e-311 with a rRNA and therefore it is an rRNA related sequence and not a P450. 
All matches to D64052 have been left out.

EST Identity Closest Family
and Subfamily
Clone name Opposite end Location in the Sequence Comments
F13573 CYP81H1 . YAY667 5' F13574 C-helix region .
F13574 CYP81H1 . YAY667 3' F13573 extreme C-terminal just past the Cys to the end .
F14118 . CYP81D VBVXH11 NA mid region 198-327 same as H77232 59% identical to CYP81D4
F14165 CYP86A7 . YBY032 5' F14166 . .
F14179 CYP71B6 . OBO6 5' F14226 . .
F14189 CYP79F1 . YAY778 3' F14190 outside coding region .
F14190 CYP79F1 . YAY778 5' F14189 N-terminal .
F14199 CYP71B6 . OBO5 3' F14178 C-terminal .
F14327 . . YAY863 5' F14331 . RNA helicase, not a P450
F15432 . . GBGF422 5' NA . Not a P450
F15479 CYP71B6 . OBO209 5' NA . .
F15574 . . GBGF814 5' F19795 . cannot align, did Blast searches on all 6 frames without any P450 hits
F19802 CYP705A19 . OBO268 3' F19864 extreme C-terminal 71% to 705A1
F19837 CYP73A5 . OBO364 5' NA . .
F19855 CYP71B6 . 3' NA . .
F19864 CYP705A19 . 5' F19802 I-K helix 74% identical to CYP705A4
69.0% identical to T88728
H36129 CYP72A11 . 174G13T7 NA . .
H36457 . CYP98A 172I16T7 AA651146 extreme C-terminal 51% identical to 98A3
H36469 CYP98A3 . 172O5T7 AA651162 . .
H36956 CYP72A14 . 181D14T7 NA . .
H37250 CYP76C2 . 179C14T7 NA . .
H76015 CYP81D8 . 193J14T7 NA K helix - heme .
H76099 . CYP94A 195C7T7 NA I to K helix 56.3% identical to 94A2
H76241 CYP71B19 . 198F7T7 NA I to K helix 59.3% identical to 71B3, 93.1% identical to T20987
H76703 CYP708A2 . 201G3T7 NA N-terminal very poor match
H76762 CYP86A2 . 202F4T7 NA . .
H76866 71A13 . 204L23T7 AA605540 I helix to PERF region 54.2% identical to 71A3
H77048 CYP87A2 . 202M10T7 NA K helix to end overlaps N37614
H77059 CYP89A5 . 202N18T7 NA . .
H77232 . CYP81D 205G20T7 NA mid 81D4 207-338 59% identical to 81D4, same as F14118
N37221 CYP90A1 . 207M21T7 NA . .
N37614 CYP87A2 . 207C5T7 NA K-helix to end overlaps H77048
N37715 CYP98A3 . 209A1T7 NA . same gene as Z35213, overlaps Z35017
N38445 CYP90A1 . 217P11T7 NA . .
N38590 CYP71A25 . 220I19T7 NA N-terminal 73.5% identical to T22148
N38616 CYP709B3 . 220P16T7 NA C-helix 38% identical to 709A1
N38687 CYP81D8 . 221K4T7 NA middle region .
N65031 CYP51A2 . 225D4T7 NA . see also T12664 Zea mays
N65153 . . 228L22T7 NA . expansin, not a P450
N65267 CYP85 . 224F7T7 NA . .
N65349 CYP71B6 . 226P8T7 NA . .
N65459 CYP83A2 . 229H10T7 NA . .
N65494 CYP90A1 . 230I2T7 NA . .
N65601 CYP73A5 . 240A11T7 NA . .
N65665 CYP71B28 . 240P12T7 NA N-terminal 72.% identical to 71B7, overlaps T14112
N65720 CYP74A . 230J8T7 NA . .
N65907 CYP706A1 . 240M22T7 NA . 60.7% identical to T22148, N65907, T44621, T46392 and T46193 are one contig
N95869 CYP86A2 . G9H9T7 NA . overlaps with T46372, T46373
N96036 CYP72A13 . F8C10T7 NA . 89% identical to CYP72A
N96214 CYP90C1 . G10G3T7 NA . .
N96740 CYP72A13 . G3E2T7 NA N-terminal .
N97058 CYP705A5 . 244D12T7 NA C-terminal 59.7% identical to T42861
N97134 CYP90A1 . 245F15T7 NA . .
R30228 CYP83A2 . 158E17T7 NA . .
R30233 CYP705A22 . 158F2T7 NA N-terminal 77% identical to 705A1
R30380 . 90C 161J16T7 NA N-term 58% identical to 90C1
R65111 CYP71B7 . 171M19T7 NA . .
R86798 CYP83A2 . 118H19T7 NA . .
R90011 CYP708A2 . 187K1T7 AA605413 mid region 46% identical to 708A1, same as T13661
R90024 CYP72A7 . 187N22T7 NA . .
R90073 CYP86A1 . 189B4T7 AA605446 . .
T04013 CYP76C2 . B71TP T04014 . overlaps T04016, Z34697
T04014 CYP76C2 . B71XP T04013 . overlaps T04015, T04017, Z33828
T04015 CYP76C2 . B71YTM NA . overlaps T04013, Z33828
T04016 CYP76C2 . B72TP T04017 . overlaps T04013, Z34697
T04017 CYP76C2 . B72XP T04016 . overlaps T04015, T04014, Z33828
T04086 CYP73A5 . SCD12T7P NA . .
T04134 CYP72A11 . 20C2T7 NA N-terminal same entry as T13009, nearly identical to T44202, differs from R90024 and H36956
T04172 CYP86A2 . 2B4T7P NA . .
T04186 CYP71B20 . 2G8T7P AA585920 C-helix to mid region 64.6% identical to 71B2 same gene as T04714
T04417 CYP705A5 . 40E11T7 NA N-terminal 72% identical to 705A2
T04442 CYP72B1 . 40H3T7 NA . .
T04452 CYP71B20 . REMOVED ? extreme C-terminal overlaps T04714, same gene as T04186
T04541 CYP71B7 . 5G6XP T04814 . .
T04613 CYP705A5 . SBB11T7P NA mid region to I helix overlaps with T45789
T04714 CYP71B20 . REMOVED ? extreme C-terminal overlaps T04452, same gene as T04186
T04814 CYP71B7 . 5G6T7P T04541 . .
T13009 CYP72A11 . REMOVED ? N-terminal same entry as T04134, nearly identical to T44202, differs from R90011 and H36956
T13661 CYP708A2 . 33B5T7 NA mid region 46% identical to 708A1, same as R90011
T14112 CYP71B28 . 47D12T7 NA N-terminal 72.4% identical to 71B7, overlaps with N65665
T14177 . CYP81D 48G12T7 NA N-terminal 62% identical to 81D6, 59.1% identical to T45449
T14186 . CYP89A 49B1T7 NA mid region 45.8% identical to 89A2
T20562 CYP86B1 . 88C24T7 AA586187 . .
T20600 CYP83A2 . 88J8T7 NA middle to I-helix beginning .
T20864 CYP74B2 . 94J16T7 AA395621 . .
T20906 CYP71B28 . 89G23T7 NA I-helix 73% identical to 71B7
T20987 CYP71B20 . 92J6T7 NA I-K helix 62.4% identical to 71B4, 93.1% identical to H76241
T21057 CYP706A1 or
CYP706A2
. 94L4T7 NA . 81.5% identical to Z24511, overlaps with T76384
T21193 CYP71B20 . 90O17T7 NA N-terminal 66.7% identical to 71A6, overlaps with T45449
T21361 . CYP89A 98A12T7 NA I helix to heme 64.8% identical to 89A2, (T76749, T44110, T21361 are all one contig)
T21786 CYP705A1 . 98D6T7 NA . .
T21800 CYP705A22 . 98F3T7 AI100646 mid region 58% to 705A3
T21833 CYP71B4 . 103J24T7 NA . .
T22148 CYP71A25 . 97O11T7 AA395671 N-terminal 61.1% identical to 71A1
T22324 CYP90A1 . 104A23BT7 NA . .
T22325 CYP90A1 . 104A23T7 NA . .
T22449 CYP72A15 . 105D3T7 NA . .
T22603 CYP72A13 . 99M7T7 NA . .
T22640 . . 105G11T7 NA . probably not a P450 accidental match
T41596 CYP81D8 . 61B4T7 AA586064 mid region .
T41670 CYP81D4 . 63B1T7 NA . overlaps with T43640
T41675 CYP90A1 . 63C12T7 AA586079 . .
T42559 CYP705A7 . 114B21T7 AA395098 I helix .
T42576 . CYP89A 114D18T7 NA I-K helix 78.8% identical to 89A2
T42716 CYP81D4 . 116D20T7 AA395149 . .
T42723 CYP81F1 . 116D9T7 NA . .
T42861 CYP705A13 . 118B4T7 NA C-terminal 68% identical to 705A3
T42902 CYP79B2 . 114O16T7 AA395121 . .
T43151 CYP90A1 . 115N3T7 NA . .
T43171 CYP707A1 . 116J23T7 NA . .
T43253 CYP98A3 . 119G2T7 NA . .
T43276 CYP705A13 . 122O1T7 AA395378 I-helix 64% identical to 705A2
T43286 CYP90A1 . 117E5T7 NA . .
T43466 CYP71B2 . 117P18T7 NA . .
T43499 CYP705A5 . 120F16T7 NA mid region to I helix .
T43640 CYP81D4 . 121M4T7 NA . overlaps with T41670
T43711 CYP88A3 . 122K4T7 NA . .
T43776 CYP73A5 . 123D6T7 AA395398 . .
T43806 CYP71A16 . 124A5T7 AA395422 mid region some resemblance to 71A
T44110 . CYP89A 122I17T7 NA I helix to heme 64.8% identical to 89A2, 74.4% identical to H77059 (T76749, T44110 and T21361 are all one contig)
T44171 CYP710A1 . 122P6T7 AA395380 . .
T44202 CYP72A13 . 123J24T7 NA N-terminal nearly identical to T13009 and T04134, differs from H36956 and R90024, at least 3 N-terminals for CYP72A
T44224 CYP71B18 . 123L24T7 NA I-K helix 67% identical to 71B4, overlaps with T44855
T44310 CYP71B7 . 123O24T7 NA . .
T44621 CYP706A1 . 127N24T7 NA . 60.7% identical to T22148 (N65907, T44621 and T46193 are one contig)
T44634 CYP83A2 . 128H9T7 NA . .
T44855 CYP71B18 . 125N6T7 NA I-K helix 67% identical to 71B4 overlaps with T44224
T44874 CYP73A5 . 126E1T7 NA . .
T44875 CYP71B7 . 126E3T7 NA . .
T45256 CYP78A5 . 83D2T7 NA I helix 64% identical to 78A2
T45449 CYP71B20 . 131A11T7 NA N-terminal 66.7% identical to 71A6, overlaps with T21193
T45663 . 89A 128O24T7 AA394479 N-terminal 38% identical to 89A4
T45669 CYP86A1 . 128P23T7 NA . .
T45691 . . 131A9T7 AA394505 . Not a P450 accidental match
T45729 CYP708A2 . 131H17T7 NA K helix to PERF region 54.7% identical to 87A1
T45789 CYP705A5 . 132D7T7 NA mid region to I helix overlaps with T04613
T45915 CYP72A15 . 133L24T7 NA . .
T46080 CYP83A2 . 135F24T7 NA . .
T46193 CYP706A1 . 138C20T7 NA . 60.7% identical to T22148 (N65907, T44621 and T46193 are one contig)
T46372 CYP86A2 . 141P21T7 NA . overlaps with T46373 and N95869
T46373 CYP86A2 . 141P22T7 NA . overlaps with T46372 and N95869
T46392 CYP706A1 . 143D20T7 AA404797 . 60.7% identical to T22148 (N65907, T44621 and T46193 are one contig)
T46441 CYP81D8 . 143P20T7 AI099973 N-terminal .
T75944 CYP83A2 . 148G2T7 AA404945 N-terminal .
T75954 . . 148I21T7 AA404948 . not a P450, matches a bacterial fragment with a P450 on it
T76255 CYP71B28 . 148I16T7 AI100027 I-helix 81.6% identical to 71B7
T76384 CYP706A1 or
CYP706A2
. 149J11T7 NA . 81.5% identical to Z24511, overlaps with T21057
T76397 CYP83A2 . 149L3T7 NA . .
T76590 CYP85 . 144I7T7 AA404883 . .
T76749 . CYP89A 150O9T7 NA I helix to heme 64.8% identical to 89A2 (T76749, T44110 and T21361 are all one contig)
T88085 CYP83A1 . 155K16T7 NA . .
T88150 CYP81F2 . 156N23T7 NA I helix to PERF region 76.1% identical to 81F1
T88278 CYP705A22 160C9T7 NA I-K helix 70% identical to F19864, 72% identical to 705A2
T88645 CYP83A2 . 160M24T7 NA . .
W43241 . CYP77 248O19T7 NA I-helix to PERF region 66% identical to 77A3
W43443 . CYP705A G11G8T7 NA N-terminal 62% to 705A9
Z17905 . . TASF037 5' NA . ribosomal protein, not a P450
Z17966 CYP706A2 . SASFb83 5' NA . .
Z17988 CYP90A1 . TASG070 5' NA . .
Z18072 CYP71B2 . VBV08-12542 5' NA . .
Z24511 CYP706A1 or
CYP706A2
. RaR086 5' NA . same as AA395860, 81.5% identical to T21057/T76384
Z25705 CYP86A2 . VCVDA12 NA . .
Z26058 CYP86C2 . GBGe199 Z26059 N-terminal 60% identical to 86C1
Z26059 CYP86C2 . GBGe199 Z26058 C-terminal .
Z26103 . CYP81 GBGe35 ? extreme C-terminal 54.1% identical to 81F1, 76.9% identical to H76015, this is the same as entries Z29010 and Z29011
Z26124 CYP90A1 . GBGe71 ? . .
Z26357 CYP86A1 . YAY044 5' Z35025 . .
Z26358 CYP86A1 . YAY044 5' Z35025 . .
Z26374 . . YAY070 5' Z27299 . this is mitochondrial DNA, probably a chimeric clone
Z27299 . CYP71B YAY070 3' Z26374 K helix to heme formerly named CYP71D3, but this is wrong
Z29010 . CYP81 GBGe35 Z29011 extreme C-terminal 54.1% identical to 81F1, 76.9% identical to H76015, this is the same as entries Z26103 and Z29011
Z29011 . CYP81 GBGe35 Z29010 extreme C-terminal 54.1% identical to 81F1, 76.9% identical to H76015, this is the same as entries Z26103 and Z29010
Z29017 CYP90A1 . GBGe71 ? . .
Z29018 CYP90A1 . GBGe71 ? . .
Z29757 . . OAO148 5' Z29758 . cannot align, probably not a P450
Z29758 . . OAO148 3' Z29757 . cannot align, probably not a P450
Z30775 77B1 . VBVAF10 Z30776 mid region 156-237 some resemblance to CYP77
Z30776 77B1 . VBVAF10 Z30775 C-term 65% identical to 77A3
Z33677 CYP71B11 . FAFJ37 3' NA I helix to end 70.4% identical to 71B1, overlaps with Z33963
Z33828 CYP76C2 . FAFK05 5' z34697 . overlaps with T04014, T04015 and T04017
Z33831 CYP83A2 . FAFK36 5' Z34720 . .
Z33855 . . GBGe381 Z34126 . this is an hsp88.1-like protein, probable chimeric clone
Z33952 . CYP71B GBGe366 5' Z34791 I-K helix 58.7% identical to H76241, overlaps Z34037
Z33963 CYP71B11 . FAFJ37 3' NA I helix to end 70.4% identical to 71B1, overlaps with Z33677 (same clone)
Z34037 . CYP71B GBGe229 5' NA I-K helix 58.7% identical to H76241, overlaps with Z33952
Z34056 CYP708A3 . GBGe258 5' Z34880 N-terminal 61% identical to 708A1
Z34126 . CYP71B GBGe381 Z33855 extreme C-terminal 49.2% identical to 71B3
Z34697 CYP76C2 . FAFK05 3' Z33828 . overlaps T04016, T04013
Z34720 CYP83A2 . FAFK36 3' Z33831 . .
Z34880 CYP708A3 . GBGe258 3' Z34056 C-term .
Z34791 . CYP71B GBGe366 3' Z33952 extreme C-terminal .
Z35017 CYP98A3 . FAI234 5' Z35213 . overlaps N37715
Z35025 CYP86A1 . YAY044 3' Z26357 Z26358 . .
Z35213 CYP98A3 . FAI234 3' Z35017 . .
Z35218 CYP71B2 . FAI244 5' NA . .
Z46540 CYP72A14 . VBVLC06 3' Z46541 . .
Z46541 CYP72A14 . VBVLC06 5' Z46540 . .
Z46573 CYP708A3 . VBVNC12 NA mid region .
AA040985 CYP97B3 . E10F11T7 NA . .
AA389789 CYP83A1 . 5' ? . .
AA394371 CYP81F1 . 306E1T7 NA . .
AA394479 . CYP89A 128O24XP T45663 C-terminal 61% to 89A2
AA394655 CYP90A1 . 162G15XP AA720263 . .
AA394869 CYP85 . 313B11T7 NA mid region .
AA394958 CYP74A . 113C3XP NA extreme C-terminal 3'UTR identical to CYP74A
AA395098 CYP705A7 . 114B21XP T42559 extreme C-terminal .
AA395121 CYP79B2 . 114O16XP T42902 extreme C-terminal .
AA395149 CYP81D4 . 116D20XP T42716 . .
AA395378 CYP705A13 . 122O1XP T43276 outside
coding
region
.
AA395380 CYP710A1 . 122P6XP T44171 extreme C-terminal poor quality sequence
AA395398 CYP73A5 . 123D6XP T43776 . .
AA395422 CYP71A16 . 124A5XP T43806 C-terminal poor sequence, some resemblance to CYP83
AA395621 CYP74B2 . 94J16XP T20864 . .
AA395671 CYP71A25 . 97O11XP T22148 C-terminal .
AA395860 CYP706A1 or
CYP706A2
. 301B12T7 NA . same as Z24511
AA404883 CYP85 . 144I7XP T76590 C-terminal .
AA404945 CYP83A2 . 148G2XP T75944 . .
AA404797 CYP706A1 . 143D20XP T46392 . .
AA585920 CYP71B20 . 2G8XP T04186 extreme C-terminal .
AA586064 CYP81D8 . 61B4XP T41596 extreme C-terminal .
AA586079 CYP90A1 . 63C12XP T41675 . .
AA586187 CYP86B1 . 88C24XP T20562 . .
AA597388 CYP704A1 . 197B8M4 5' NA . .
AA597575 CYP81F1 . 201M10T7 NA . .
AA597583 CYP706A1 or
CYP706A2
. 202A15T7 NA . .
AA597644 CYP706A1 or
CYP706A2
. 203M2T7 NA . .
AA598000 CYP81F4 . 249I22T7 NA extreme C-terminal .
AA598015 CYP84A1 . 249K6T7 NA . .
AA605413 CYP708A2 . 187K1XP R90011 C-terminal 63% identical to CYP708A1
AA605446 CYP86A1 . 189B4XP R90073 . .
AA605540 CYP71A13 . 204L23XP H76866 . .
AA650762 CYP71B18 . 282B12T7 NA I-K helix .
AA651059 CYP51A2 . 169K20XP AA720360 . .
AA651146 . CYP98A 172I16XP H36457 N-terminal .
AA651162 CYP98A3 . 172O5XP H36457 . .
AA651564 CYP90C1 . 273E2T7 NA . .
AA657317 CYP73A5 . 118J10T7 NA . .
AA712696 CYP86A1 . 178H22T7 NA . .
AA712784 CYP81F3 . 198C15T7 NA PERF region to end .
AA713019 CYP85 . F5F3T7 NA . .
AA713328 CYP708A2 . 250B12T7 NA N-terminal 66% identical to 708A1
AA720028 . CYP71B 134N10T7 NA N-terminal 65% to 71B13
AA720263 CYP90A1 . 162G15T7 AA394655 . .
AA720360 CYP51A2 . 169K20T7 AA651059 outside coding region .
AA728483 . CYP704A K2F4T7 NA PERF region to end 54% identical to CYP704A2
AA728684 CYP98A3 . K4B10TP NA . .
AI099821 CYP71B18 . 125N6XP T44855 . .
AI099973 CYP81F4 . 143P20XP T46441 . .
AI100027 CYP71B28 . 148I16XP T76255 C-term 70% to 71B7
AI100386 CYP81F1 . 116D9XP T42723 . .
AI100632 CYP71A25 . 97O10XP T22147 extreme C-term 2 diffs with
AA395671
AI100646 CYP705A22 . 98F3XP T21800 C-term .
AI100749 CYP71B4 . 103J24XP T21833 . .
AI138147 CYP706A1 . sp2 NA C-term or 706A2