Arabidopsis Cytochrome P450 GSSs

Family Assignments

Last modified Sept. 7, 1999


Revised Sept. 7, 1999 31% of GSSs are still not identified(45/145).  This fits well with the 29% of the genome not yet sequenced on Sept. 7, 1999.

Revised Aug. 3, 1999 32% of GSSs are still not identified(47/145).  This fits well with the 33% of the genome not yet sequenced on Aug. 3, 1999.

Revised July 8, 1999 33% of GSSs are still not identified.  This fits well with the 34% of 
the genome not yet sequenced on July 8, 1999.

Currently there are 145 GSSs. 
The assignments made below are subject to change when longer fragments become 
available.  I have tried to be as careful as possible in making assignments, but I cannot 
foretell the future.  All percents refer to protein sequence identities.

Comparisons between percent identities are not meaningful unless the fragments cover the 
same region of the P450 sequence.  The C-terminal is more highly conserved than the N-
terminal and this is more conserved than the middle of the sequences.  30% identity in the 
middle may be significant while 50% might be low at the C-terminal.

GSS Identity Closest Family
and Subfamily
BAC name Opposite end Location in the Sequence Comments
AL093403 CYP71A15 . T11H3 . . .
B25921 CYP71A21 . F1B9 NA I helix 55% to 71A14
AL080661 CYP71A21 . F1I22 . . .
AL096411 CYP71A22 . T16P17 . . .
B11433 CYP71B2 . F11K5 B10996 mid-K 95% to 71B2
B12376 CYP71B2 . F10K10 B09520 mid-K 95% to 71B2
B12539 CYP71B4 . F20C19 B08387 . .
B10642 CYP71B4 . F20C19 B10463 . .
B27880 CYP71B6 . T1K24 B27879 . .
B28631 CYP71B6 . T14L18 B28632 . .
AL095273 CYP71B6 . T14L18 . . .
B18782 CYP71B8 . F2A3 B19332 I-PERF 44 aa exact match
AL089388 CYP71B8 . T3M21 . . .
B28673 CYP71B11 . T15N16 B28674 . .
B78204 CYP71B11 . T31K18 B78205 . same seq. as B28673
AQ011301 CYP71B11 . F26L12 AQ011300 . same seq. as B28673
AL095836 CYP71B11 . T15N16 . . .
AL087348 CYP71B11 . F14D13 . . .
AL089222 CYP71B13 . T3H8 . . .
AL084167 CYP71B13 . F8E24 . . .
B25347 CYP71B14 . F26G21 B98172 mid 95% to 71B14
B25335 CYP71B18 . F26E21 NA mid 72% to T04186
AL092574 CYP71B21 . T10E9 . . .
AL092575 CYP71B21 . T10E10 . . .
B96063 CYP71B24 . F23G3 B96062 mid 68% to 71B3
AQ011201 CYP71B24 . F23D14 AQ011200 I-heme 84% identical to 71B3
B11396 CYP71B27 . F13G13 B11353 mid-K 63% to 71B2
B21019 CYP71B27 . F25B19 B20553 K-PERF 92% to B11396
AL093252 CYP71B28 . T11D21 . . .
B21027 . CYP71B T14N21 B10963 PERF-heme 79% to 71B7
B21125 . CYP71B T32B12 B20387 Nterm-C helix 71% to 71B15, 86% to N65665
B22554 . CYP71B F20H2 B22555 mid 76% to 71B9
AL092788 . CYP71B T10J6 . Nterm - mid 78% to 71B8
AL081333 . CYP71B F2M19 . PERF to end 6 diffs with 71B8
B24203 CYP72A12P . F19F16 B24202 C-term 97% to 72A12P
B29839 CYP72B1 . T17P14 B29840 . .
B62694 CYP72B1 . T17P24 B29841 . .
B28185 CYP72B1 . T4A14 B28186 . .
B20052 . CYP72B T16M6 B19864 C-helix 79% to B27750 43% to 72B1
B27750 . CYP72B T16M6 B27751 C-helix 79% to B20052 41% to 72B1
AL084053 CYP76C2 . F8B14 . . .
AL088340 CYP76C3 . T1M7 . . .
B97241 CYP77A4 . T32M21 B97240 Nterm 68% to 77A3
AL082681 CYP77A4 . F5F6 . . .
AL083526 CYP77A4 . F7B10 . . .
B24360 . CYP77A F17L2 NA Nterm-C helix 66% to 77A3
B25606 . CYP77A F28F12 NA mid - heme 68% to 77A3, 69% to B30234
B30234 . CYP77A T30L12 B29284 Cterm 70% to 77A3
AQ011443 CYP78A7 . F27J20 AQ011442 . .
B25471 CYP78A7 . F27J20 NA mid 96% to 78A7
B61504 . CYP79A T17K21 B61505 PERF-end 80% to 79A2
AL093101 CYP79F1 . T11A9 . . .
B20058 CYP79F2 . T17K15 B19870 . .
B61499 CYP79F2 . T17K15 B61500 K-helix 94% to 79F2
B19924 . CYP79F T11A9 B20082 K helix 75% to 79F1
AQ250603 CYP81D2 . T8H13 AQ250602 . .
AL091699 CYP81D2 . T8H13 . . .
B22310 . CYP81D F18I8 NA mid 72% to 81D3
B21261 . CYP81D F18I8 B21229 mid 60% to 81D3, 80% to B22310
AL090945 CYP81F4 . T6O13 . . .
B26848 CYP81H1 . T21F2 B26849 . .
B28869 CYP81H1 . T21E12 B28870 . .
B29081 CYP82F1 . T25L24 B30092 . .
B27454 CYP82F1 . T8J23 B27455 . .
B20125 CYP82F1 . T8J23 B19904 Nterm 85% to 82F1
AL095159 CYP83A1 . T14I2 . . .
B09951 CYP85 . T1F9 B08108 mid region exon 1 diff with 85
AL088478 CYP86A1 . T2A13 . . .
AL089168 CYP86A8 . T3F17 . . .
B19916 . CYP86 T9M21 B20137 C-helix region 46% to 86A1
B09869 . CYP86 T16I7 B12024 Nterm-mid 82% to 86A4
B97734 . CYP86 F16P10 NA C helix - mid 43% to 86B1
AL084336 . CYP86 F8K5 . C-helix to mid 47% to 86B1
same as B97734
B12879 . CYP86 T16G4 B09271 mid 54% to 86A4
B09868 . CYP86 T16G9 B12880 mid 53% to 86A4
B08375 . CYP87 T2C7 B08374 C-term 71% to 87A2
B10696 . CYP87 T2C7 NA C-term 68% to B08375, 56% to 87A2
B12705 CYP88A3 . F28P8 B11525 . .
B12104 CYP88A3 . F9C12 B11685 . .
AL088126 CYP88A4 . T1G2 . . .
AL088480 CYP88A4 . T2A14 . . .
B11817 . CYP88 T12O12 B10395 Nterm 83% to 88A3
B11581 . CYP88 F25F21 B08973 K-heme 64% to 88A3
AL087677 CYP89A4 . F14M11 . . also = 89A7
AL087931 CYP90A1 . F15D22 . . .
B77695 CYP90B1 . T28K14 NA K-helix 98% to 90B1
B25656 CYP90C1 . F28K17 B25877 . .
AQ011208 CYP90C1 . F23E13 AQ011207 . .
B96406 . CYP90 F18O14 B21480 mid 36% to 90C1
B97796 . CYP90 F18O14 B97795 mid same seq. as B96406
B21696 . CYP90 F15A8 B21477 mid 76% to B96406/B97796
AL088834 CYP93D1 . T2L17 . . .
AL081067 CYP94B1 . F2E21 . . .
AQ010792 . CYP94B F20J3 B22627 mid to I-helix 51% to 94B1, 85% to B97734
AL080732 . CYP94 F1L6 . mid to I-helix same as AQ010792
B96393 CYP96A4 . F18M20 B97776 . .
B20346 . CYP96 F18J1 B21146 C-helix 86% to B22314, 52% to 96A2
B22314 . CYP96 F18J1 B22313 C-helix 86% to B20346, 46% to 96A2
B98666 . CYP96 F19O3 NA Nterm 92% to 96A4
B28642 CYP97B3 . T15A12 B29664 . .
B09856 CYP97B3 . T15A12 B09141 . .
B67502 . CYP97B T24M9 NA C-helix 46% to 97B3
B26976 . CYP97B T24M9 B26975 C-helix same seq. as B67502
AL080882 . CYP98 F1O17 . Nterm - mid same as AA651146
B61390 CYP701A3 . T22K1 B61389 . .
AL087395 CYP701A3 . F14E19 . . .
AL087968 CYP702A2 . T1A22 . . .
AL094730 CYP702A2 . T13L21 . . .
B24420 CYP702A5 . F20B11 B24421 . .
AQ249305 CYP702A5 . T20I13 AQ251471 . .
AL087352 CYP704A2 . F14D15 . . .
B97399 CYP705A3 . T33J16 B97400 . .
B97581 CYP705A6 . F15L11 AQ011060 . .
B97148 CYP705A7 . T32F24 B78382 . .
B97150 CYP705A7 . T32G12 B78384 . .
AL090286 CYP705A7 . T5J19 . I-helix to heme 93% to B97148
AL090287 CYP705A7 . T5J20 . I-helix to heme 89% to B97148
AL090622 CYP705A15 . T6E23 . mid to K-helix 84% to 705A4
AL088383 CYP705A20 . T1N16 . I-helix to heme .
AL095325 CYP705A21 . T14N22 . mid to I-helix 74% to 705A2
B74035 . CYP705 T27J15 B74034 Nterm 78% to 705A2
B73988 . CYP705 T27F21 NA Nterm same seq. as B74035
B96118 . CYP705 F23P4 B96117 Nterm 71% to 705A3
B77649 . CYP705 T28H12 B77650 Nterm 77% to 705A2
AL091537 . CYP705 T8C3 . Nterm same as B77649
B95908 . CYP705 F23A4 B95907 I-PERF 59% to 705A2
AQ011073 CYP706A1 . F15M19 B97597 . or CYP706A2
AQ010732 CYP706A1 . F19J4 NA . or CYP706A2
B97603 CYP706A1 . F15N10 AQ011075 . or 706A2
B62508 CYP706A2 . T22A21 B62509 . .
AL086483 CYP706A4 . F12L13 . . .
B29124 CYP707A1 . T26L24 B30127 . .
AL090955 CYP707A1 . T6O19 . . .
B12307 CYP707A2 . F16C1 B09512 K-helix .
AQ010620 CYP707A3 . F26K1 AQ010619 . .
AQ010555 . CYP707 F24F8 AQ010556 C-term 93% identical to CYP707A3
B67654 CYP707A4 . T23M15 NA K-heme 71% to 707A1
B98253 CYP707A4 . T22P20 B98252 K-heme same seq. as B67654
B12886 . CYP708 T17B10 B11383 Nterm 93% to 708A1
B09878 . CYP708 T17B6 B11188 Nterm 75% to 708A1
B13024 . CYP708 T29G23 B08684 Nterm 83% to 708A1
AL089622 CYP711A1 . T4F8 . . .
B25676 CYP713A2 . F28M23 B25675 . .
B96503 CYP714A1 . F23A22 B96504 . .
AL083283 CYP715A1 . F6J7 . . .