Agaricus bisporus P450s

 

D. Nelson

 

August 9, 2009

 

5  Sequences (not a complete genome set)

 

>CYP66A1 Z82021 Agaricus bisporus (a mushroom)

WSYINSIVANSLWNSVKERMEAGTAKPCIATAMLEDLLDDDSAE

SKEEETVRRGACANGFLGGADTTVSLVTSFFMAMALYPDVQKKAQAELDQVLGGRLPE

FSDRPSLPYVNALLKESERWQPVFPLAIAHMSSNADEYDGYYIPKGTYVIGNAWSILH

DPEFYKDPLVFNPDRFLKDGEIDPSVRDPNVASFGFGRRICPGRFFPDASLYSTVTHV

LTVFDIKPNLDENGKEIGIKPDMTDGLLSIPM

 

>CYP66B1 Agaricus bisporus partial mRNA for cytochrome P450 (cyp gene).

AJ271707

50% to CYP66A1

QLKSVRTFIRNVMESPDEFSEWIHFYTSSSIMEIIYGMKAKPED

PYVDNAKKAIEGFNEAAVPGKFLVETFPVMKHIPSWFPGAGWKRQALFWRDVNREVRV

RPFNLVKDQVNEGTATRSVCRTLIGNLPDSTAPDRIVKENIAIDTCAVSFIGAAETSH

SAARVFFMAMLMNPEVQKKGQAELDKVLNGRLPEPNDGPNLPYISAMVKETLRWQLVL

PLAVPHVAIEADEYNGYYIPKGTIVFGNSWTFMHDPEVYKDPESYMPERFLKDGKLDS

SIRDPSTAVFGYGRRICPGRYFALNALYLMIAHTLAVFDIKPALDENDNEKEFKADVT

GGMISQPVPFQCMIVPRSKAAADLIQNSDLME

 

>CYP699A1    Agaricus bisporus (mushroom)

            GenEMBL AJ312129

            Eastwood,D.C., Kingsnorth,C.S., Jones,H.E. and Burton,K.S.

            33% to CYP502A1, 34% to 620E1

MPILSLARDILFISLCGLIYRIFRNRRNRLLPLPPGLLRWPILG

NALSMPLTYAHIYYKHLGNKLGSKFIYLEAVGQPFLIINDYRVAKDLLEERSAIYSRP

QFPMSTEVIGLKRFFLLMPYGDIWRNHRRVFRQYFDPKYLPRDQEKQLNFIRKSLLPN

LFIAPEEAHEHVANCIGGFILSIVYGLPVKTRHDRLVRFAEKTFNDLAASTAPGKYLV

NIIPQLQYLPDWFPGTEFKRVALKTAEELDKLREEPYQAVLIAMVGHVVQNCLVSDNV

EKLRNAPDFEDRIVDVKEVARTIFGAGFETTTGVIMTFVLAMLLNNDVQLQAQSEIDA

VLGPERLPTFSDLSDLPYFSAVIKEVLRWNPIGPIGIPHATTNEDEYDGYYIPKDCIV

VSNIYAMLHDENIFPDPEKFDPGRFMKNGTTINDDLLNPIDVVFGFGRRVCPGSHLAM

SMLEITAASILCLFDILPALDAQGKPIDVVPEFTPASITSHPLPFKFNVSPRKGKDVE

GLLSEYMNVEYA

 

>CYP699A2 Agaricus bisporus

partial mRNA for cytochrome P450 (cyp gene).

AJ271708

84% to 699A1, 42% to CYP502 Coprinus cinereus AB013443

ENVEKLCNAPDFDDRIAEVKEVARAVFAAGFESTTGNIMTFILA

MLKNHDVQRQAQSEIDSVLGIERLPTFSDLSDLPYFSAVIKEVLRWNPPVPIGVPHLT

TDEDVYDGYYIPKSCIVMSNIYAMLQDETVFPDPEKFDPGRFIKNGDMVNDELLNPKD

VIFGFGRRVCPGSHLAISMLEITAASILCLFDILPALDTQGKPIDVVPEFTPASITSH

PLPFKFTFSPRKGKDVEGLLSEFMNVEYA

 

>CYP? AJ534343

38% to CYP6001C8 Nectria haematococca

SSSIYWICIRRFTPRPCLPRYYRVATKDQTIGKNELKKGDRVFA

DIGSANLNEKMFSEPSKVDPSRSTSACIFGDGPLRFLGETLTVKITSQVLRAIYAYNN

VRRAPGASGKLKRFTEGSHPQYHFAYLDRDFKISPWPTSLTIQYDA