CYP3 clan (56 sequences in 13 bins)

 

$$$$$$$

 

>fgenesh2_pg.scaffold_7000240|Brafl1 41% to CYP3A5 (no allele)

MLELLPLSPTWVLVGVCLVLYYLYLTWPFYTFKRLGIPGPKPVVNFGNILSYTEGSHVFDCKCYKQYGKVFGFFEGRTPM

LMVGDLELIKEITVKQFNNFSNRRDLGSLLEAFAGGLFVIEDTDWKRVRSAISPTFSSSKLKQMSALVEKCADGLVSSLM

EKQEKRVMFDMKELSSAFSMDVISSTAFGVEVDSFKHPDHPFATHAKAIFKINMLKGFFYVMLPPPFNHIFKLRGFSLFP

KFATDFFKQTVHNVIKMRRGNPDDDGLSVVVPCRMTKPSQCLSMTEIVGNSLAFWLAGYDTTANTIALTAYNLAFNQEAQ

DKVREEIDRVVQARGKLDYEALNEMRFLEMCVNETLRIYPGAKRFDRVCKEDIEVKGLHIPAGTIINIPAYAIHHDPEIW

PEPEKFRPERFSKEQQESRDPYAFLPFGSGPRACVGMRLAMLEIKHALAKVLEKFRFVTCEKTEVPLRLENNNGNQVTGG

VWLKVEARTHTPRVRMSSA*

>fgenesh2_pg.scaffold_1716000001|Brafl1 42% to CYP3A27

98% to fgenesh2_pg.scaffold_7000240|Brafl1

MTEIVGNSLAFWLAGYDTTANTIALTAYNLAFNQEAQDKVREEIDRVVQARGKLDYEALNEMRFLEMCVNETLRIYPGA

(gap) 46% to CYP3A27

KRFSKEQQESRDPYAFLPFGSGPRACVGMRLAMLEIKHALAKVLEKFRFVTCEKTEVPLRLENNNGNQVTGGVWLKVEART

HTPRVRMSSA*

>fgenesh2_pg.scaffold_507000029|Brafl1 42% to CYP3C.b N-term

99% to fgenesh2_pg.scaffold_7000240|Brafl1

MLELLPLSPTWVLVGVCLVLYYLYLTWPFYTFKRLGIPGPKPVVNFGNILSYTEGSHVFDCKCYKQYGKVFGFFEGRTPM

LMVGDLEIIKEITVKQFNNFSNRRDLGSLLEAFAGGLFVIEDTDWKRVRSAISPTFSSSKLKQMSALVEKCADGLVSSLM

EKQEKRVMFDMKELSSAFSMDVISSTAFGVEVDSFKHPDHPFATHAKAIFKINML

KGFFYVMLPPPFNHIFKLRGFSLFPKFATDFFKQTVHNVIKMRRGNPDDDQESRDPY

60% to CYP5A danio

AFLPFGSGPRACVGMRLAMLEIKHALAKVLEKFRFVTCEKTE

>estExt_fgenesh2_pg.C_70236|Brafl1 green = 35% to CYP3A5

67% to fgenesh2_pg.scaffold_7000240|Brafl1

MAAMLHLLPLSPTWVLLGVCLLFYYLYVRRRCILLCSISVPFFVFFCYLTWPFRTFKKLGIPGPKPVLNFGNFLSYKK HI

TNNIISGQHISTNNIISGQHSSANNTISGRTTTTQLPAVLMTTECVEAPVEYRNVPTCVCQRGKQLAADGRTCEDVDECA

LSPPVCGDPSVTHATCENKPGYYLCNCQSGYVMDVDRKACTLNSGGWGPWSDWGPCSKSCDLGMQSRTRVCDSPAPSFGQ

PTCQGSSTGVQMCAENNCPSDATERENAVFLKFKDMPAGEYPMVEAALRAKVSNLVNRYCRGRGAFPICCPEASEDQ GIY

EGRQPILMIGDLGLIKEITVKQFHKFNNRQDLGSILKPFAGSLFVLKDTDWKRVRGAISPTLSSSKLKQMFVLVEKCADG

LVSSLTQGRRALIDMKGCNIYLFCRIFSAYAMDVISSTAFGVDIDALNNPDHPFVTHAKAIFDMNLLKMYLYIMVPPPFN

YLFTLWGFTFFPKFATDFFKHTVERVMKMRQDSADNTERVDFLQLMLKAHNKHRDETANGATLGPGLSKQEIVGNSMIFW

LVGYDTTANTIALTAYNLALSQEAQDRAAGEIHRVVQNRGKLDYEAVHELHYLEMCVNETLRIYPGAKRFDRVCREDADI

NGLHIPAGMNINFPVWVIHHDPELWPEPDKFKPERFSKEEIATRDPYAFLTFGVGPRACVGMRLAMLEIKLALAKVLEKF

RFVTCEKTEDH*

>estExt_fgenesh2_pg.C_7730004|Brafl1 48% to CYP3C.b

81% to estExt_fgenesh2_pg.C_70236|Brafl1

MAAMLPFLPLSPSWVLLGVCLLLYYLYLTWPFRTFKKLGISGPKPVLNFGNFLSYKKGSHVFDMDCYKKYGKVYGIYEGR

QPILMIGDLGLIKEITVKQFHKFNNRQDLGSILKPFAGSLFVLKDADWKRVRGAISPTLSSSKLKQ ERVDFLQLMLKAHN

KHRDETTSGGTLGHIKLVASTGNRIVDKELDKVIA*

 

$$$$$$$

 

>e_gw.406.5.1|Brafl1 37% to CYP3A5 no allele

MFTSVLPRPQGLSNIDLEWMKKYGKYWGVYEGQLPVLIVADTKLIKQINVKEFPNFANRRLMPGNGPVMKYSLTVLQDAE

WKRVRSYMSPFNSAYSLKQLCYLIENTSDNLVAAMKRYHDAGQYVDVKEIFGCYTMDVISSTGFGTDVNSLSDPDSIFIK

NVKKFYAIGALSPFTLLTFGFPWFAFFLDRNNWFFNIVPPPVFNFFADAIRKVISIRESNPAESDKRVDVMQLLLKSHNT

ALDEPGNEGNIKHGLSYNEILANGFIFWIGGYDTTATTISFLAYNLALNPDIQERVIAEIDEIMRGRECMDYKAASEMKY

LKMCVDETLRMYPPSQRFDRQAKEDIDLDGVKIPKGMCVQFSSFAIHYDPDNWPDPEKFDPERFTPEEKKKRDPYAYVAW

GVGPRSCVMKRLGMLEVKFAIAKILMKYRLRPCEKTQIPIRVKVSNLTQPDHGMFLKLEARTDI*

 

$$$$$$$

 

>fgenesh2_pg.scaffold_157000010|Brafl1 35% to CYP3A7

no allele next best match is 52%

MTTDEWNLLSLTYGLLLLFIILFCIYVYRTMSVLRRLGVSGPSPLPFVGNFLTYRKGQRAAFAEWCQKYGRVYRIFEGLN

PKLVVSDLEMVKQILVMDSTSFPFRKPFPLPIGLPAHGLTFAPFIPDHQRVRGVLTPAFSPEKIKMMVPLINKCANHLME

NLEKMCSEGTTFDVKRVFGCFSMDVIASTAFGVDVNSHKNPQDAFVENARKIDITTFTALPPYLVIGGILSYFLPKYVLQ

KLGFAIFPADVVSFFRGVFDDIVDERSKQPPDQRRVDLLELMTRSNEDKIHKHLKDDEGERGVAGLSPDEMFSQVVAVFT

AGYSNSSGLLANTALYLTKNPDVMDKLQREVASDLPTLCQRHDDEVDNRPRRYRSRG*

 

$$$$$$$$$$

 

>e_gw.106.108.1|Brafl1 45% to CYP4A11

MWKMLKWGQTDSITLYLCLIILVFRFLRALLWKRYIQKVLAPFPGQPAHWLFGHMREVRPDETGFTIVPQWAAKFKFAFP

LWIGPFRVVLSLVHPDYIKEIVNSPEPKDRVSYAWLKPWIGDGLLVSEGQKWFRNRRLLTPGFHFDVLKPYVKVFSECTN

IMLDRWADLAPGTPVEMFHYASAMTLDSLMRCALSVRSDCQRDSDGSPYIRAVYDLTKCVVERGRFPPFHIPLIFHLSPT

GFRFRKACKTAHDFSDEVIRKRRTELQQQGCHQNDTANSSEDGGKKRYLDFLDILLQARDEDGKGLSEREIRDEVDTFMF

EGHDTTASGVSWILYNLAKHPACQDRCRAEVDAVLQGRAEVKWWDLSKLPYTTMCIKESLRMHSPVPGVTRLTTQPHTFP

DGRSIPAGVSVSIGVHSLHHNIHVWGDNVMEFDPERFSPENSKGRSSHAFIPFSAGSRNCIGQHFAMNELKVTVALTLQR

YRLELDETRPPYRVARLITRTRDGLWLKVYPRGADN*

>fgenesh2_pg.scaffold_785000007|Brafl1

95% to e_gw.106.108.1|Brafl1

MVSIPAQWLPHWETGYLRTACLTVLVAVAVQLVFRFLRALLWKRYIQKVLAPFPGQPAHWLFGHMREVRPDETGFTIVPQ

WAAKFKFAFPLWIGPFRVVLSLVHPDYIKEIVNSPEPKDRVSYAWLKPWIGDGLLVSEGQKWFRNRRLLTPGFHFDVLKP

YVKVFSECTNIMLDRWADLAPGTPVEMFHYASAMTLDSLMRCALSVRSDCQRDSDGSPYIRAVYDLTKCVVERGRFPPFH

IPLIFHLSPTGFRFRKACKTAHDFSDEVIRKRRAELQQQGCHQNDTANSSEDGGKKRYLDFLDILLQARDEDGKGLSERE

IRDEVDTFMFEGHDTTASGVSWILYNLAKHPACQDRCRAEVDAVLQGRAEVKWEDLSKLPYTTMCIKESLRMHSPVPGVT

RLTTQPHTFPDGRSIPAALECIILHHNIHVWGDNVMEFDPKRFSPENSKGRSSHAFIPFSAGSRNCIGQHFAMNELKVTV

ALTLQRYRLELDETRPPYRVARLITRTRDGLWLKVYPRGTDN*

 

$$$$$$$$$

 

>estExt_fgenesh2_pg.C_1760080|Brafl1 43% to CYP3A5

MLDLFFFPVTCGLFGLCAVLIYMYLMWPYSTFEDLGIPGPKPLPLFGNYLSYGKSAGEFDRECYKKFNKVYGIFEGRQPI

LMVGDLDLIKDITVKESNNFTNRRVLSGQGEILSKGLTALKDDDWRRFRSTISPAFSSVKLKQMALVIEKYADNFVASLD

KNAKQDVMFNMKELSGAYTMDVISGTAFGVNVDSLHDLNSPFVSNAKDRFNISLFDPFMITISLFPRLGKLLQNLGVTLY

PIKAVEFFRAAVDNVIDMRGRNEDEAKRVDFLQLLLKAHNEELDEEVSKEVISKHGVSKKDIKRESVLFWLGGYETTANT

ISLTTYNLAVHQEVQDKVIQEVDAIIKKHGKLDYDAVNDLHYLDMCVNETLRLFPASQRFERVNKEDTEIRGLKIPAGTI

VNIPAYAIHRDEEIWPDPEDFKPERFSPEEKESRDPYAFLPFGSGPRNCIGMRLAQLELRFALAKALQKFRFVPCDKTVI

PIRIKNTLSNQIEGGVWLKVEARA*

>estExt_fgenesh2_pg.C_1410072|Brafl1 green = 42% to CYP3A4

98% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLRSFKKHVEGLSLEPKPEHSPGKLEEKIFYCKLCCLESTRARAHTFFTFEEGLKHLRNHWKPPKQQRGPPIRRNKVPPE

LYYKQKAVNVEEDITLLAKLYLGFKVQERNPYRCGLCGEVFPTTSNRYHHVEAEHVEVCVPLLCCGAAFQNRDKYIQHMF

FKHCELRIQTGKESLLICKLCFVAFYNRTDLEWHYYRHNQHVDNPTLDSGDGPFPTRSQFFRCTLCSDQCVLSRRKPPVL

YTLSESFSHLGWHWLHQIPPEIRQNPVLMFEDDADIIHKLYMDPVGMQAWQCGICPLQLNKEHVLNYHSEGRRVLRCEGV

TCKNSLQFAKLEDFVGHIFMRHTEVLKLEEMFLCLICNRILCGLIPHSRILESPARSLCRCLGTTYHTERI FEGRQPILM

VGDLDLIKDITVKESNNFTNRRVLSGQGEILSKGLTALKNDDWRRFRSTISPAFSSVKLKQMALVIEKYADNFVASLDKN

AKQDVMFNMKELSGAYTMDVISGTAFGVNVDSLHDLNSPFVSNAKDRFNISLFDPFMITISLFPRLGKLLQSLGVTLYPI

KAVEFFRAAVDNVIDMRGRNEDEAKRVDFLQLLLKAHNEELDEEVSKEVISKHGVSKKDIKRESVLFWLGGYETTANTIS

LTAYNLAVHQEVQDKVIQEVDAIIKKHGKLDYDAVNDLHYLDMCVNETLRLFPASQRFERVNKEDTEIRGLKIPAGTIVN

IPAYAIHRDEEIWPDPEDFKPERFNQEEKESRDPYAFLPFGSGPRNCIGMRLAQLELRFALAKSLQKFRFVPCDKTMIPI

RIKNTLSNQIEGGVWLKVEART*

>e_gw.7.651.1|Brafl1

69% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLDLLPFSPTWVLVGLCVVLIYLYMVWPYSTFKKLGIPGPKPVLLFGNYLSYRKGGSKFDKECYKKFNKVYGIFEGRHPL

LMVGDLELVKDITVKEFNTFTNSYRRTFEGQEGSVLTVLKDADWKRVRSAISPAFSPGKLKQMEMVVEKCANNFVVNLVE

KAKQGVVFDLKELSGAYTMDVISGTAFGVHLDSLHDRNHPFVANAKKMFDVNLFHPIIIILFLFPPLVKVLQKLGASMFP

REVLDFFSAAVDNVMDMRGSNEDEAKRVDLLQLMLKAHNEELDKEVPKDVVKHGQEIKDNAMLFWLAGYETTADTLSLTA

YNLALHQEAQNKVIEEVDAIVEKRGKLDHDAVNEMPFLEMCVNETLRIFPVNQRFDRVCKEDIEVKGLHIPAGTVVAIPA

YAIHHDPEIWPEPEKFKPERFTKEEKESRDPYAYLPFGSGPRNCVGMRLALMELKFALAKALQKVRFVTCDKTVVSME*

>estExt_fgenesh2_pg.C_70234|Brafl1 55% to CYP3A4

90% to e_gw.7.651.1|Brafl1

MLDLLPFSPTWVLAGLCAVLLYLYMVWPYSTFKKLGIPGPKPVPLFGNYLSYGKGVSKFDKECYKKFNKVYGIFEGRQPI

LIVGDLELVKDITVKEANTFTNRRIFEGQGDIIGNGLTILKDADWKRVRSAISPTFSSGKLKQGKLDYEAVNEMHFLEMC

VNETLRMFPAAQRFDRVCKEDTEVKGLHIPAGTIVNIPAYAIHHDPEIWPEPEKFKPERFSKEEKESRDPYAYLPFGSGP

RNCVGMRLALMELKFALAKALQKVRFVTCDKTVIPIGIKNTLGNQIEGGMWLKVEARS*

>fgenesh2_pg.scaffold_1518000001|Brafl1 44% to CYP3A7

82% to e_gw.7.651.1

MALVVERCGDNFVANLEENAKQGVGFDLKELSGAYTMDVISGTAFGVDVDSLHNPNHPFVAHAKKLFDFNLFNPFVIILC

EYLVSIHVGKVLEKFGVSLFPRKVLNFFSAAVDNVIDMRGSNEDEAKRVDFLQLMLKAHNKELDKEVPKDVVKQGVSKKE

IKGNAVLFWIAGYETTANTISLTAYNLALNQEAQDKVIEEVDAIVEKRGKLDYEAVNEMHFLEMCVNETLRMFPAAQRFD

RVCKEDTEVKGLHIPAGTIVNIPAYAIHHDPEIWPEPEKFKPERFSKEEKESRDPYAYLPFGSGPRNCVGMRLALMELKF

ALAKALQKVRFVTCDKTVIPIGIKNTLGNQIEGGMWLKVEART*

>e_gw.773.9.1|Brafl1 47% to CYP3A27

94% to fgenesh2_pg.scaffold_1518000001|Brafl1

EIKSNAVLFWIAGYETTANTISLTAYNLALHQEAQDKVIEEVDAVIEKRGQLDYEAVNEMPFLEMCVNETLRMFPAAQRF

DRICKEDTEVKGLQIPAGTIINIPAYAIHHDPEIWPEPEKFKPERFSKEEKESRDPYAYLPFGSGPRNCVGMRLALMELK

FALAKALQKVRFVTCDKTVVSHWLDEASSPKAK*

>e_gw.7.15.1|Brafl1 385 to CYP3A5

76% to e_gw.7.651.1|Brafl1

MLDLLPFSPTWVLVGLCAVLLYLYMVWPYSTFNDLGIPGPKPVPLFGNYLSYGQGIAKFDKECYKKFNKVYGIFEGRQPI

LIVGDLELVKDITVKEANTFTNRRVKGQGEFFDGGLLNIKDADWKRVRSAISPTFSSGKLKQMALVVEKCGDNFVANLEN

NAKQGVMFDLKDLSGAFTMDVISGTAFGVDVDSLHDPNHPFVANARKLFDFNLFNPILIVLFLFPQLAKIFSAMGLNMSV

FSTKALEFFGAAVDNVLDMRGSNEEEAAKRVDFLQLMLKAHNEELDKEMPKDVEIKSNAVLFWIAGYETTANTISLTAYN

LALHQEAQDKVIEEVDAVIEKRVSPKSNLTTRTPATTHVTNSNMMAFCATGNNFPPMHQKSSQWCVSFRTHSAKSAPKTA

KMPPAMFSKEEKESRDPYAYLPFGSGPRNCVGMRLALMELKFALAKALQKVRFVTCDKTVVSILLDEASSLKAE

>fgenesh2_pm.scaffold_321000003|Brafl1 41% to CYP3A27

62% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLELLPFSPTWVLVGLCALFLYMYLVWPFSTFKKLGIPGPKPVPLFGHYLEYGKGINIFDQECYQKYNKIYGIFEGRQPI

LMIGDLDLVKAITVKGINTFTNHRDMPGQGEGKHALFFSKDETWKRLRIAMSPAFSSGKLKQLTVHIERCAEGFVSNLAE

DAEHGKVFDVKELCGAFSMDVISSTAFGVEIDSLHNPDHPFVTNAKAIFDISLFNIAFFIMFAFPQLGKFLERFGMTVLP

KKPMKFFSEAVDSVMDMRKTNSGDEKPVDFLQLMLKAHNEELDNKASGEVAKHAGVSKKEIKGNAVLFWVAGYDTTANTL

TLTVYNLAVDQKAQDRAVQEVDAIIAKRGKLDYESVNELTYLEMCINETLRMFPASQRFDRVCKKDTEVNGLHIPEGTIV

TFPVWAIHYDADIWPEPEKFKPERFSKEEKESRDPYAFLPFGAGPRNCLGMRIALLELKFALAKALQKFRFVTCEKTELV

LQDFRTPVGVKYATKQKGPSF*

>estExt_fgenesh2_pg.C_3610051|Brafl1

57% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLALLPVPPTWGLLVGLFPVLFYLFLVWPFSTFKKLGIPGPKPIPLFGNYLDYLKGTNLFDQECYNKYNKVWGIFEAQTP

ILMAGDLKLVKEITVKRTNIFQNRRDLGGRGEIFSASMAALKDADWKRVRGAISPTFSTGKLKQMSPHVEKCANDFVSKL

AENEKEGTMFDMKWISGGYALDVISSTAFGVQVDSLNNPDHPLVTSAKTLFVFNFFNPLILIVFLFPQLAWLLEVCGVSF

MNRKSINYFSEAVDNAISMRKAKQADGASDEARPPDFLQLMIEAHNAELDNGVSKDTFKYGVSKREIKGNAVLFWVAGYE

SSSNILSLTAYNLALHQDVQDKAIEELDAVIRKHGKLDYKAVHELPYMEMCINETLRLYPTALRIDRVCTEDVDIHGVHI

PAGMLCFVPVWTIHHDPDIWPEPEKFRPERFSKEEVEARDPYAYLPWGSGPRNCAGMRIAQLELRFAMAKALEKFRFFPC

EKTEIPVRFKNSFIHQVDGGVWLKVEARAHHA*

>e_gw.7.201.1|Brafl1 42% to CYP3A5

99% to estExt_fgenesh2_pg.C_3610051|Brafl1 (allele)

WILILTSLNINRFLVWPFSTFKKLGIPGPKPIPLFGNYLDYLKGTNLFDQECYNKYNKVWGIFEAQTPILMAGDLKLVKE

ITVKRTNIFQNRRDLGGRGEIFSASMAALKDADWKRVRGAISPTFSTGKLKQMSPHVEKCANDFVSKLAENEKEGTMFDM

KWISGGYALDVISSTAFGVQVDSLNNPDHPLVTSAKTLFVFNFFNPLILIVFLFPQLAWLLEVCGVSFMNRKSINYFSEA

VDNAISMRKAKQADGASDEARPPDFLQLMIEAHNAELDNGVSKDTFKYGVSKREIKGNAVLFWVAGYESSSNILSLTAYN

LALHQDVQDKAIEELDAVIRKHGKLDYKAVHELPYMEMCINETLRLYPTALRIDRVCTEDVDIHGVHIPAGMLCFVPVWT

IHHDPDIWPEPEKFRPERFSKEEVEARDPYAYLPWGSGPRNCAGMRIAQLELRFAMAKALEKFRFFPCEKTEVKS*

>estExt_gwp.C_3530067|Brafl1

61% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLDLLLFSPTWALAGLCAVLLYLYLVWPLSTFKKLGIPNPKPVLLFGNYLEYWKGVNLFDKECYAKYNKVYGYFEGRVPN

LMVGDLEIIKEITVKEINKFTNRRPLPGQGEIFDNSLLGLKDADWKRVRSAITPTFSSGKLKQMAAQIEHCAERLVASLA

ENQKKGTEFDMKHLAGAFTMDVISSTGFGVDVDSLHNPDHPLVTSAKSFFEFNFYDPKAIILFLFPSLFGILERFGITLF

PKKALDYFKEAVDSVISMREGESNGKRPVDLLQLMLQAHNEELDKKHGISSMEFKGNAIILWVVGYETTANTISLTAYNL

AVNQEAQGKVHAEIDALLEKREKLDYDAVHDLPYLDMCISETLRMYPAATRFDRICKEDTEVKGLHIPASMIVSVPVYAI

HHDADLWPEPEKFRPERFTKEEKESRDPYAYLPFGAGPRICVGMRLAQLELKFALAKMLQKFRFVTCDKTVVSEDLRTQ

>estExt_GenewiseH_1.C_70558|Brafl1

80% to estExt_gwp.C_3530067

MLDLLPFSPTWVLVGLCVVLVYLYMVWPYSTFKKLGIPNPKPYPLFGNYLEYGKGANLFDKECYEKYNKVYGYFEGRTPN

LMVGDLELIKEITVKEINKFTNRRTFDGQGDIFDNALTSLKDTDWKRVRSAITPTFSSGKLKQMAAQVERCADGLVANLA

ENQKTGMEFDMKRLAGAFTMDVISSTGFGVDVDSLHNPDHPLVTSAKSFFEFNFYNPMIIILFLFPSLGGILERLGFTFF

SKKALNYFSEAVDTAVSMRGSEPNGTHGISSIEIKGNAIIMWVAGYETTANTLSLTTYNLALHPEVQEKVHAEIAALLEK

REKLDYEAVNDLPYLDMCISETLRMYPAAQRFDRICKEDTEVKGLHIPAGMIVNIPVYAIHYDAELWPEPEKFKPERSED

EGKSRDPYAYLPFGSGPRNCVGMRLAQLELKFALAKMLQKFRFVTC

>fgenesh2_pg.scaffold_361000050|Brafl1

95% to estExt_GenewiseH_1.C_70558|Brafl1

MALPIVEDVNKDLEGLQALMWLNSPILALHTLLHCWQLNWAEETVWSTASSSSHLPIILKGLCAPPCQSSPDDFVSCSIF

PVSFRDYRDDILLCYCDLYDCVIIYDFTTSLA

GAFTMDVISSTGFGVDVDSLHNPDHPLVTSAKSFFEFNFYNPMIIILF

LFPSLGGILERLGFTFFSKKALNYFSEAVDTAVSMRGSEANGTEK

(gap) 51% to 3A4

LDYEAVNDLPYLDMCISETLRMYPAAQRFDRICKE

DTEVKGLHIPAGMIVNVPVYAIHYDADLWPEPEKFKPERFTKEEKESRDPYAYLPFGSGPRNCVGMRLAQLELKFALAKM

LQKFRFVTCDKTDIPVRLQNTLGNQIEGGLYLKVEART*

>fgenesh2_pg.scaffold_64000080|Brafl1

61% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLELLPFSPTWVLVGLCALFLYMYLVWPFSTFKKLGIPGPKPVPLFGHYLEYGKGINIFDQECYQKYNKIYGIFEGRQPI

LMIGDLDIVKAITVKGINTFTNHRDMPGQGEGKHALFFSKDETWKRLRIAMSPAFSSGKLKQLTVHIERCAEGFVSNLAE

DAEHGKVFDVKELCGAFSMDVISSTAFGVEIDSLHNPDHPFIKFHAYHCSLFPKVAFPQLGKFLERFGMTVLPKKPMKFF

SEAVDSVMDMRKSNSGDEKPVDFLQLMLKAHNEELDNKASGEVAKQAGVSIKEIKGNAVLFWVAGYDTTANTLTLTAYNL

AVDQEAQDRAVQEVDAIIAKRGKLDYESVNELTYLEMCINETLRMFPASQRFDRVCKEDTEVNGLHIPEGTIVTFPVWAI

HYDADIWPEPEKFKPERFSKKEKESRDPYAFLPFGAGPRNCLGMRIAMLELKFALAKALQKFRFVTCEKTEKFPVLSKFV

QNCG*

>estExt_gwp.C_6870007|Brafl1

60% to estExt_fgenesh2_pg.C_1760080|Brafl1

MLDLLLFSPTWALAGLCAVLLYLYLVWPLSTFKKLGIPNPKPVLLFGNYLEYWKGVNLFDKECYEKYNKVYGYFEGRVPN

LMVGDLEIIKEITVKEINKFTNRREYGLKTCPMQSINGFRYADWKRVRSAITPTFSSGKLKQMAAQIEHCAERLVASLAE

NQKKGTEFDMKHLAGAFTMDVISSTGFGVDVDSLHNPDHPLVTSAKSFFEFNFYDPKAIILFLFPSLFGILERFGITLFP

KKALDYFKEAVDSVISMREGESNGTRPVDLLQLMLQAHNEELDKKHGISSMEFKGNAIILWVVGYETTANTISLTAYNLA

VNQEAQGKVHAEIDALLKKREKLDYDAVHDLPYLDMCISETLRMYPAATRFDRICKEDTEVKGLHIPAGMIVSVPVYAIH

HDAELWPEPEKFKPERFTKEEKESRDPYAYLPFGAGPRICVGMRLAQLELKFALAKMLQKFRFVTCDKTVVSEDLRTQ

 

$$$$$$$$$$

 

>fgenesh2_pg.scaffold_226000040|Brafl1 37% to CYP5A1

MGVPGPRPWPVFGDFFGQMIRGFWAPDYQVQMMRQYGEVYGVFSGSNPVLMVSDPEMLREIFVKQFHKFSRRNPEGMTLR

TTPQGRYLINLSGEDWKNVRTTLTPAFSSGKLKLMLEQLNRCANILAETVGESAKTGKPFKARELTGGFTMDAIASTAFG

TEIDSQRNPEDPFVIHGKQGLKFDFKNPVVWLFIFFPKIMKPILEAINWDFFPKKSYKFFESVFDQLMAMRHHGGIERVD

FMQLMANAHKDKGEDDEDMGEVKVQGQRKALTKADVVSNGILFFLAGYDTTSLTMAYTMYNLALNQEAQDDVIREVDDVM

EGKDDIDNEALGQLTYLEMCIMETLRLYPPGANVMTRCCVEDAVVNGLSIPKGMSILVPIQGIHYDPERWPEPKKFIPER

FTKEEREKRNPFHWLPFGAGPRNCIGMRLALMEAKIGLAKVFMKYRILPCAETQVPLKIKKHEVFPAAEDGVMLKATPRD

RSDD*

>e_gw.29.216.1|Brafl1

91% to fgenesh2_pg.scaffold_226000040

MYVNNCFLSLSLLQLLFLAPQRYYIRPLSLFKKMGVPGPRPWPVIGDFFGQMIRGFWAPDYQVQMMRQYGEVYGVFSGSN

PVLMVSDPEMLREIFVKQFHKFSRRNVSWLFTFIVTVSTCVMSLGEDWKNVRTTLTPAFSSGKLKLMLEQLNRCANILAE

KVGESAKTGKPFKARELTGGFTMDAIASTAFGTEIDSQGNPEDPFVIHGKQGLKFDFKNPVVWLFICFPKIMKPILEAIN

WDFFPKKSYKFFESVFDQLMAMRHHGGIERVDFMQLMANAHKEKEEDKEDMGEVKVQADVVSNGILFFLAGYDTTSLTMA

YTMYNLALNQEAQDNVIREVDDVMEGKDDIDNEALGQLTYLEMCIMETLRLYPPGANVMTRCCVEDAVVNGLSIPKGMTI

LVPIQGIHYDPERWPEPKKFIPERFTKEEREKRNPFHWLPFGAGPRNCIGMRLAMMEAKIGLAKVFMKYRILPCAETQVP

LKIKKHEVFPAAEDGVMLKATPRDKSEN*

 

$$$$$$$$$$

 

>estExt_fgenesh2_pg.C_230022|Brafl1 33% to CYP3A5

MALEFLPIPLSWVVLIVLPVLFYIHSLFVLLSLSLLLALRRRFLTSVLSCLILWGLLLVDVWLDLNRRRRRQLHHHLRSL

PILQPGLALTTGTTTYCVRPLSMFKKMGVAGPKPYPVIGNMMEFKNGLWDIELQMSRVKEFGGAYGVFGGMRPAYVVTDR

EFLREIFIKQFHNFADRSASTTALEVRPMCRALTDLRGDVWKIVRTTISPSFTGGKLKLMTDILNRCADQLVDNIGTSTR

DGAACDIKELSSAFTMDAIAAVAFGTDINTQETPDHPFVVNAKKAFGMDLKNPLLWVYLILPKITKPIMESLQVSLMPRD

CVNFFSGVLSQLMQQRQAGGEGRVDMMQLMMDAHTMRDEEEGEGGEAGGRTRGRKQALSRDDVLANGIQFILAGYETTAS

TLSFTMYNLATHPDVLDKVIREVDDVMKDRDTVDYEACKKLEYMEMCIMENLRIYPAAPRLARVATADTKIQWLTVPKGM

LMDIPVLPIHYDPERYPEPDKFIPESLPIHYNPERYPEPDKFIPERFSKEERQKRDPCDWLPFGAGPRNCIGMRLAMMEA

KIALAKILRSYRIALAPDMEVPVQLSKYTAFVKPEHGIRITVEPRNSGGD*

>gw.94.110.1|Brafl1

80% to estExt_fgenesh2_pg.C_230022|Brafl1

LTVTLSYCVRPLSMFKKMGIAGPRPYPVIGNLTEFKTVSVNFDYVSKSNMVVFGGMRPAFVVTDREFLREIFIKQFHNFT

DRSVSSNSVDVRPMCRALADLRGDDWKNIRTTLSPSFTGGKLKLACTNILNSCADQLVDNIGTSTRGGAACDIKELSSAF

TMDAIAAVAFGTDINTQETPDHPFVVNAKKAFSVDLKNPLVWVYLLVPKIVKPIMEGLQVGLVPRDCVNFFSDVFTQLME

QRQAGGEGRVDMMQLMMDAHNMKDEEGEGGEAGALSRDDVLANGFQFILAGYETTASTLSFTMYHLATHPDVLDKVIQEV

DDVMKDRDVVDYEACKKLEYMEMCIMENLRIYPAAPRLARVATADTKIQWLTVPKGMLMDIPVLPIHYDPERYPEPDKFI

PERFSKEERQKRDPCDWLPFGAGPRNCIGMRLAMMEAKIALAKILRSYRI

 

$$$$$$$$

 

>estExt_GenewiseH_1.C_4310012|Brafl1 40% to CYP3A4

MDVIARTAFGTEIDSQRNPQDPFVLMGRKAFNVEFSSPILMLFVMFPSIMKPIMEWLNIDLFPRDATSFFYSIFDQLIGL

RQKSAEKGRVDFLQLMMDAHKDADEEEEDGVKSHGQKHALTRDDIVANGFLFFVAGYETTASTMAFALYNFALNQEEQDK

AREEVNNIMMDRDLVDYEAVHEMSYLEMCCMETLRLYPPAPNIGRVVSEEVKVQWLTIPKDMFVLVDVLAIHYDPERWPE

PNKFIPGRFTKEAREKRDPYNWLPFGAGPRNCIGMRLAMMELKVGLAKILMKYRFMTAPDTDIPLKMKKTNQFPLPENGI

RLKVELIDQDEN*

>estExt_gwp.C_3360044|Brafl1

86% to estExt_GenewiseH_1.C_4310012|Brafl1

MALEFLPVPLSWVLLALLAVLFYLYAVRPLWVFKKMGVPGPTPLPLLGNMLTIMNDGIWNPEASRQRVQLYGKVYGIFFG

RSAILTISDPEMLRDIFVKKFHALSNTQVATKPSGRMLINLRGEDWKDVRSTLSPAFSGGKLKMMMPAINTCADQLVQNI

AKFAETGESFDTKEVVRLTSTEVLCAVRTGWTSQLVVLESRCYITPSFLCFRFILESPSLISLFMFPSIMKPILEWLNID

LFPRDATSFFYSIFDQLIGLRQKSAEKGRVDFLQLMMDAHKDADEEEEDALTRDDIVANGFLFFVAGYETTATTMAFALY

NFALNQEEQDKAREEVNKIMENRDLIDYEAVHEMSYLEMCCMETLRLYPPIPNTGRVASEEVKVQWLTIPKDMMVIVPVI

AIHYDPERWPEPKKFIPERFTKEAREKRDPYNWLPFGAGPRNCIGMRLAMMELKVGLAKILMKYRFKTAPDTDVRTELYL

VS*

>estExt_GenewiseH_1.C_4310016|Brafl1

86% to estExt_GenewiseH_1.C_4310012|Brafl1

MALEFLPVPLSWVLLALLAVLFYLYAVRPLWVFKKMGVPGPTPLPLLGNMLTIMNDGIWNPEASRQRVQLYGKVYGIFFG

RSAILTISDPEMLRDIFVKKFHALSNTQTDAVTLDTKPSGRMLINLRGEDWKDVRSTLSPAFSGGKLKMMGPAINTCADQ

LVQNIAKFAETGESFDTKEVLGEFSTRLNAFEARGATGQLVVLESHCYTTPSFPFFRFILESPSLISLFMFPSIMKPILE

WLNIDLFPRDATSFFYGIFDQLIGLRQKSDEKGRVDFLQLMMDAHKDADEEEEDALTRDDIVANGFLFFVAGYETTATSM

AFALYNFALNQEEQDKAREEVNKIMENRDLIDYEAVHEMSYLEMCCMETLRLYPPIPNTGRVASEEVKVQWLTIPKDMMV

IVPVIAIHYDPERWPEPKKFIPERFTKEAREKRDPYNWLPFGAGPRNCIGMRLAMMELKVGLAKILMKYRFMTAPDTDVR

AEL

>e_gw.336.47.1|Brafl1

76% to estExt_GenewiseH_1.C_4310012|Brafl1

YAVHPLWTFKNMGVPGPTPLPFVGTLLRWFKDVSRISCGHSQYGKVYGFFFGRTPTLIISDGEMLREIFVKKFHALTDRQ

TDTLSLDTKPSGRMLINLRSEEWKNVRSTLSPAFSGGKLKMMGPAINTCADQLVQNIAKFAGTGESFDTKKLTGAFTMDV

IARTAFGTEIDSQRNPQDPFVLMGRKAFNVEFSSPILMLFGGSVSMRVLNVEHLVPNACFMYIPLDNINTRVLDSVAKGR

VDFLQLMMDAHKDADEEEEDGVKSHGQKHDDIVANGFLFFVAGYETTASTMAFVMYNFALNQEEQDKAREEVNKIMEDRV

GLLLLLLVQNKKVSNTIVSHYRTWLTMRHIGRVVSEEVKVQWLTIPKDMFVLVDVLAIHYDPERWPEPNKFIPERFTKEA

REKRDPYNWLPFGAGPRNCIGMRLAMMELKVGLAKILMKYRFMTAPDTDVRTELYNS*

 

$$$$$$$$$

 

>estExt_fgenesh2_pg.C_1550032|Brafl1 38% to CYP3A7

MACESLPMSVSWGLLAALICLVLLYGYRTMSVLQKLGVRGPTPLPFVGNLLTYRKGQNVAFREWRQKYGRVYGMYEGLAP

KLVITDPEIVKQVFVKDFSNFPMRMPLCIPIGLPEETLIFADFSHHKRRRGILSPAFTAGKVKLMVPLVNRCADFLMENL

DKLFVEETTFDAKQVFSYLTLDVMASTAFGVEVNSQKDPQHAFVVNARKVDLSVVGGMKPSLIIAAVLSMVIPMDVLEKW

GLTVVPADAVSFFHSILETILTMRIKQQPNERRMDLLELMRAAREEHCLDLPEEFEKSLSADEVTTDAISFFLAGYDTIA

GLLGTTAVNLARYPDMQDKLLQEVATVTQEQDYVQNEDIQKMEYLDMFLMETLRMDSGARFPRLCTQDTEISPGLTVPAG

VEVIIDSDSLHIDPEYWPEPGTFIPERF

TKEESNKREPFTFIPFGQGPRTCIGMHLALMATKITLVRILQKYRFVACPGTPKDHGLGFFVRNQWVKLERFDY*

>estExt_fgenesh2_pg.C_4440045|Brafl1 32% to CYP3A27

97% to estExt_fgenesh2_pg.C_1550032|Brafl1

MACESLPMSVSWGLLAAFICLVLLYGYRTMSVLQKLGVRGPTPLPFVGNLLTYRKGQNVAFREWRQNYGRVYGMYEGLAP

KLVITDPEIVKQVFVKDFSNFPMRMPLCIPIGLPEETLIFADFSHHKRRRGILSPAFTAGKVKLMVPLVNRCADFLMENL

DKLFVEETTFDAKQVFSYLTLDVMASTAFGVEVNSQKDPQHAFVVNARKVDLSVVGGMKPSLIIAAVLSMVVPMDVLEKW

GLTVVPADAVSFFHSILETILTMRIKQQPNERRMDLLELMRAAREEHCLDLPEEFEKSLSADEVTTDAISFFLAGYDTIA

GLLGTTAVNLARYSDMQDKLLQEVATVTEEQGYVQNEDIQKMEYLDMFLMETLRMDSGARFPRLCTQDTEISPGLTVPAG

VEVIIDSDSLHIDPEYWPEPETFIPERTSCEN

APGCFIQQNHEDYVTMEPLVDQSEIDVSDIFDFVAESVVRWKDLARKL

ELSEAKIGRTDAEQRGNVKESCIEILETWRLKEGCNATVEQGGGIARDLVAKMGVDDVTVLVEMDDNDLEKARTFFNELD

VEQLETLTITEGSRTTATTGDTDSGGAMSSSGTGDSSAQPRLRTKSGDTLGTSPRPHLAECTKCSAMNEDLQSYLKCSTG

HLICRKCFAEHIQTNPAVSVNCPLCSQDIGPYLKASEHTTKDSVWIFVVDSNLWVQGKKLIGKERKFKVAEDPRIRIDVG

QLSEIVAEGRQVVTGTLYGSEPPKLDTIWKKVRQRGWRVPQPKKRSQFTGKEKQIDNQLGVDVTSLVCKTEEGKRGTVIL

VTGDADVVPVLENILHEPPWKTEIWMWEKSISKEIRSLQQCHPDRMRIEHLDAYSKRLTFTNYTYHINPGKHNQLLTQAI

VFEGVQKQPDSLKMNVKWVKRMEEVCRWPFQYAWIFEDNNDINDLLVVFIKGKDAEGTFRGPDLAEMLDLVKKDPYLHSV

STNVQAYFSYKHSRAMDDSVNICLSNQYSGLTDMYDKVEQLEDEGFHSLEEEKEDETTIQVPFPIHQRGARPHLQQFPVQ

PTYRQPSRPGARQHRPFYHPPVTSQAMTPWSPTTEASGAWIPAPMKNRQSPYRPRYSEHCPRKFNCGRGRHCEYTHTPEE

LDFLKQRPPGLKVYKVKLCDKWKTGYCMSEARACNWAHGEGDASCLRCNCRGHLTDNCPTYM*

 

$$$$$$$

 

>estExt_fgenesh2_pg.C_920015|Brafl1 38% to CYP3A43

MALEVLPIPLTWTLLPVLAVLFYLFFNGMTPALVISDAEMMKEIFVKQFHKFTNRQTDSQMINVKPLSRMVFQLLDDDWK

NVRTTLSPAFSSSKLRQMTGAMNSCAEQLVENISSFATTKESFDVRQLTGAFTMDVIARTIFGTEINSQRNPQDPFAINA

KKSSNINFNSPLIWLYLLFPGTMKRICELLDYQLFFPREATDFFNGVMDQLLAMRETDGGKGRVDMLQIMIDAHKEKDED

STDGAKVHGKKQPLTRDDVVANGIGFFNAGYDTVSITLSFLLYHLALDQEIQDKVRQEIHDVTDGESLVDYEAVQKMSYL

EMCVMETLRLYSLVPFLVRVAAEDVQLKDLTIPKGMGVSVPVLAIHYDPARWPEPRKFIPERFTKEEREKRNPYDWMPFG

AGPRNCVGMRLAMMELKVGVAKILMKYRVTTGPDTQVPVKLNKWQQFPTPESPIRLMVEPVE*

>e_gw.285.122.1|Brafl1 32% to CYP3A43

87% to estExt_fgenesh2_pg.C_920015

GMTPVLMISDAEMMKEIFVKQFHKFTNRQMINVKPLSRMVFQLLDDDWKNVRTTLSPAFNSSKLRQVRTLSSIKNEGLIF

TQPLLHFHRPSVALLSAMNGCADQLVENISSFATTKESFDVRQLTGAFTMDVIARTIFGTEINSQRNPEDPFVINAKKSS

NINFNSPLIWLYCKICEFLDYQLFFPREATDFFNGVMDQLLSMRETDGGKGRVDMLQIMIDAHKEKDEDSTDGAKVHGKK

QRMNCSKSICWSLTRDDVVANGIGFFNAGYDTVSITLSFLLYHLALNQEIQDKVRQEIDDVTDGESLVDYEAVQKMSYLE

MCVTETLRLYSLVSGLTRVAAEDVNLKGLTIPKGVGVFVPVVAIHYDPARWTDPRKFIPERLDLYSSSSLQIV*

>estExt_GenewiseH_1.C_290099|Brafl1

95% to estExt_fgenesh2_pg.C_920015|Brafl1

MALEVLPIPLTWTLLPVLAVLFYLYAVRPLFIFRKMGVPGPTPLPVIGTTYLTAFWGFYNNNISVSDVGPVKGFFNGMTP

ALVISDAEMMKEIFVKQFHKFTNRQTDSQMINVKPLSRMVFQLLDDDWKNVRTTLSPAFSSSKLRQMTGAMNSCAEQLVE

NISSFATTKESFDVRQLTGAFTMDVIARTIFGTEINSQRNPQDPFAINAKKSSNINFNSPLIWLYLLFPGTMKRICEFLD

YQLFFPREATDFFNGVMDQLLSMRETDGGKGRVDMLQIMIDAHKEKDEDSTDGAKAHGKKQHDVVANGIGFFNAGYDTVS

ITLSFLLYHLALDQEIQDKVRQETYQVTDGESQVDYEAVQKMSYLEMCVMETLRLYSLVPFLVRVAAEDVQLKDLTIPKG

MGVSVPVLAIHYDPARWPEPRKFIPERFTKEEREKRNPYDWMPFGAGPRNCVGMRLALMELKVGVAKILMKFRITTGPDT

VVPVKLNKWQQFPTPEIPIRLMVEPVD*

>gw.1094.3.1|Brafl1 46% to CYP5A, 43% to CYP3A27

94% to estExt_GenewiseH_1.C_290099|Brafl1

VDYEAVQKMSYLEMCVMETLRLYSLVPFLVRVAAEDVQLKDLTIPKGMGVSVPVVAIHYDPARWTDPRKFIPERFTKAER

EKRNPYDWLPFGAGPRNCVGMRLALMELKVGVAKILMKYRVTTGPDTVVRL

 

$$$$$$$$

 

>e_gw.516.10.1|Brafl1 38% to CYP3A5

MAVDVLPIPLTWVLLGLLVLLLYIYGVRPLSTFKKMGVPGPRPWPVIGNLLDRFRHGIYNPDFQLEISQKYGKVYGHYRG

AATPLLVISDNEMLREVFVKQFHNFSQRIVNRMMTLVNGEEWKNMRSTLSPAFSAGKLKQMTEQVNHCADQLVSNLGEQT

SQGHLTGGFAMDAIASVAFGTDIDSQRNPNDPFVVHAKKAFEGQMKNPLFWLFMFFPWIMKPLLEGLGYNFFPRSTTDFF

YKVLDQLMELRQTTRLDFMQLMLNAHKEPEEEEEDSTLSKDDVVSNGIVFFLAGYDTTATTMAFALYNLAVNQEAQDKVI

QELDEVMREKDQVDHEAVQQMTYLEMCIMETLRLHPPGAIFTTRICTRDTTIQWLKIPKGMTVVVPVLAIHYDPERWPEP

KKFIPERFTAEEREKRDQYDWLPFGAGPRNCIGMRLAMMEAKIGLAKVFMKYRIRTGPDTDVSM

>estExt_gwp.C_5160028|Brafl1

83% to e_gw.516.10.1

MAVDLLPIPLTWVLLGLLVLLLYIYGVRPLSTFKKMGVPGPQPWPLIGNLLDVRHGVYNPDFTLERSQKYGKVYGLYRGA

ATPLLVISDREMLREVFVKQFHNFSTRTGDTQFLRTKPLDCMLSVVNGEKWKSLRSTLSPAFSAGKLKKMSEQLNRCADQ

LVSNLGEQTSQGQSFEAKKLTGDFTMDAIASVAFGTDIDSQRNPNDPFVVHAKKAFEGPFKNPLFWLFLFFPWIMKPLLE

GLGYNFFPRSTADFFYKVLDQLMELRQTTVSVSERLDFMQLMLNAHKEPEEEEEDNSTDVKVQGQKQALTKDDVVSNGIV

FFLAGYDTTATTMAFTLYNLAINQEAQDKVIQELDEVMREKDQVDHEALQQMTYLEMCIMETLRLHPPAALIMTRICTKD

TTIQWLKIPKGMTVLIPVLAIHYDPERWPEPKKFIPERFTAEEREKRDQYDWLPFGAGPRNCIGMRLAMMEAKIGLAKVF

MKYRIRTGLDTDVSRTMLKFNAF*

>e_gw.6.166.1|Brafl1

58% to e_gw.516.10.1|Brafl1

MSADSLPVPLTWILLAILPVLFYIYAVRPLQLFKKMGVPGPAPWPIIGNLLDHMRDGIWNIETQVARTRKYGTVYGMFGG

GFTGVFITDPEMLQDVFVKKFHSFTNRQINVRPAGRMLTSLFDEDWKSVRSTVSPAFSGGKLKQMAGPMNSCADLLIENM

SKFAESGECFEVKELTGAFTMDAITRTAFGTQIDSQKNPDDPFVVYAKKMIDRPISPALLMFMFFPKLMKPIFEKFQVGI

FPTAPTQFFYNVFDQLMEMRETEQERVDFMQLMMNAHKEHDKEPDGSGDKPPLTKDDVVANGILFFIAGYETTATTMAFA

LYNLALNQDVQEKVRQEIEHVMEDKVLVDYEAVHKMSYLEMCILETLRMYPPVSINMRASSEEVKLKWLTIPKGMLVGVP

ILSIHYDPERWPEPYKFIPERFTKEEKEKRGAFDWVPFGSGPRNCIGMRLALFELKLGLARLLMKYRVMTGPDTDVRMGL

ILV*

>estExt_fgenesh2_pm.C_1370013|Brafl1 39% to CYP3A7

77% to e_gw.6.166.1|Brafl1

MAGDSLPIPLTWILLAILPVLFYIYAVRPLQLFKKMSVPGPTPWPIIGNLLDHMKDGVWNIESQVERRKKYGSVYGMYGG

GLTAIFITDPEMLQDIFVKKFHSFTNRQPKDIHLRVKPVGRMLTQLVDEDWKNVRSTISPAFSGGKLKQMAGAMNSCAGL

LVENIGKYADTGESFEVKELTGAFTMDVITRTAFGTQIDSQKNPDDPFVVNARRLIDRPISIFALMFMFFPRLVGSLLER

FQYNLLPADATQFFYSVFDQLMELRQAEGQERVDFLQLMMNAHKEHDKEHDDTGEARQHSHKQPLSKDDVVANGILFFIA

GYETTATTMAFTLYNLALNQDKQDRLRQEITQVMADREFVDNEDVHKMPYLEMCISETLRMYSPAAMTTRASSEEVKLKW

LTIPKDMLVAAPILAIHYDPERWPEPYKFIPERFTKEEKEKRGPYDWMPFGAGPRNCIGMRLALFELKLGLARLLMKYRV

MTAPDTDGGRNSLLAPWS*

>estExt_fgenesh2_pg.C_4700007|Brafl1 35% to CYP3A7

97% to estExt_fgenesh2_pm.C_1370013|Brafl1

MAVDSLPIPLTWILLAILPVLFYIYAVRPLQLFKKMGVPGPTPWPIIGNLLDHMKDGVWNIESQVKRRKKYGSVYGMYGG

GLTAIFITDPEMLQDIFVKKFHSFTNRQPKDIHLRVKPVGRMLTQLVDEDWKNVRSTISPAFSGGKLKQMAGAMNSCAGL

LVENIGKFADTGESFEVKELTGAFTMDVITRTAFGTQIDSQKNPDDPFVVYARRLIDRPISIFALMFTLTKDDVVANGIL

FFIAGYETTATTMAFTLYNLALNQGKQDKLRQEITQVMADREFVDNEDVHKMPYLEMCISETLRMYSPAAMTTRASSEEV

KLKWLTIPKDMLVAAPILAIHYDPERWPEPYKFIPERFTKEEKEKRGPYDWMPFGAGPRNCIGMRLALFELKLGLARLLM

KYRVMTAPDTDIPLKMKKFKKFPIPENGIRLKVELVDSVTY*

>estExt_gwp.C_3490086|Brafl1 34% to CYP3A27

84% to e_gw.6.166.1|Brafl1

MSADSLPVPLTWILLAILPVLFYIYAVRPLQLFKKMGVPGPAPWPIIGNLLDHMRDGIWNIESQVARTRKYGTVYGMFGG

GFTGVFITDPEMLQDVFVKKFHSFTNRQTKDLQINVRPAGRMLTSLFDEDWKSVRSTVSPAFSGGKLKQHRKIEPMIKEF

HCCTLKYIVKNVTHKGMMSCCYRLTGAFTMDAITRTAFGTQIDSQKNPDDPFVVYAKKMIDRPISPALLMFMFFPKLMKP

IFEKFQVGIFPLHPPTSSRLSTSQAHRVDSLNYTPLLSGCKLMYPHFSALTKDDVVANGILFFIAGYETTATTMAFALYN

LALNQDVQEKVREEIEHVMEDRVLVDYEAVQKMSYLEMCILETLRMYPPVSINMRASSEEVKLKWLTIPKGMLVGVPILS

IHYDPERWPEPYKFIPERFTKEEKEKRGAFDWVPFGSGPRNCIGMRLALFELKLGLARLLMKYRVMTGPDTDVRMGLILV*

>estExt_gwp.C_860113|Brafl1

83% to e_gw.516.10.1

MAVDLLPIPLAWVLLGLLVLLLYIYGVRPLSTFKKMGVPGPRPWPLIGNVLDVRHGVYNPDFTLERSQKYGKVYGLYRGA

ATPLLVISDREMLREVFVKQFHNFSTRTGETQFLMTKPLDCMLSTVTGEKWKNLRSTLSPAFSAGKLKKMSEQLNRCADQ

LVSNLGEQTSQGQSFEAKKLTGDFTMDAIASVGFGTDIDSQRNPNDPFVVHAKKAFEGPFKNPLFWLFLFFPWIMKPLLE

GLGYNFFPRSTTDFFYKVLDQLMELRQTTVSVGERLDFMQLMLNAHKEPEEEEEDNSRDVKVQGQKQALTKDDVVSNGIV

FFLAGYDTTATTMAFALYNLAINQEAQDKVIQELDEVMREKDQVDHEALQQMTYLEMCIMETLRLHPPAALIMTRICTKD

TTIQWLKIPKGMTVLIPVLAIHYDPERWPEPKKFIPERFTAEEREKRDQYDWLPFGAGPRNCIGMRLALMEAKIGLAKVF

MKYRIRTGPDTDVSMTMLKFNAF*

>e_gw.86.22.1|Brafl1

91% to e_gw.516.10.1

YGVRPLSTFKKMGVPGPRPWPVIGNLLDRFRHGIYNPDFQLEISQKYGKVYGHYRGAAAPLLVISDNEMLREVFVKQFHN

FSQRIVSRETKTKPVDRMMTLVNGEEWKNMRSTLSPAFSAGKLKQMTEQLNHCADQLVSNLGEQTWSQGHLTGGFAMDAI

ASVAFGTDIDSQRNPNDPFVVHAKKAFEGQMKNPLFWLFMFFPWIMKPLLEGLGYNFFPRSTTDFFYKVLDQLMELRQTT

TDTEKLDKYMSLTLYSIFFCSFLSKERLDFMQLMLNAHKEPEEEEEDSSRDVKVQGQKQALTKDDVVSNGIVFFLAGYDT

TATTMAFALYNLAINQEAQDKYQGEVHEVRVYEDQVDHEALQQMTYLEMCIMETLRLHPPGAIFTTRICTKDTTIQWLKI

PKGMTVVIPVLAIHYDPERWPEPKKFIPERFTAEEREKRDQYDWLPFGAGPRNCIGMRLAMMEAKIGLAKVFMKYRIKTG

PDTDVSMIMFNSF*

 

$$$$$$$

 

>estExt_GenewiseH_1.C_90137|Brafl1 38% to CYP5A

MELDFLPIPLTWVLLAALSVLFYLYCMRPMNMLKKMGIPHPKPLPVIGNMHQTMKTGVWNPDAQVERINNFGAVYGSYMG

RTVYITISDPEMLREVFVKQFHKFTNRAPDGQSLNIKPSCRMMTQLVDEDWKNVRSTLSPAFSGGKLKQMSEAMNTCADV

LLKNLGKFAEKGESFDSKDLTSGFTTDVIARTAFGLEVDSQGNPEDPFVVYTRKPFAVAFRNPLFWLFFLFPKIMKPILE

FFEYSFLDKDVSSFFYNVVDQVMGMRQTEDKAHRHVDFMQLMMNAHKEDDDDDNKEEGTQVQGIKQPLTRDDIVANGFLF

FIAGYETTATTMGFLLYNLALHQEAQDKAREEINQVMEGRELVDYEAVNKMSYLEMCIHETLRMYSPASGVVRVVGDEVK

MKWLTLKKGMNVMIPILGIHNDPKRWPEPKKFIPERFTKEEREKRDQYDWLPFGAGPRNCIGMRLALMELKVGVARVLRK

YRIVTGPDTDV

>estExt_fgenesh2_pg.C_14420002|Brafl1

99% to estExt_GenewiseH_1.C_90137|Brafl1

MELDFLPIPLTWVLLAALSVLFYLYCMRPMNMLKKMGIPHPKPLPVIGNMHQTMKTGVWNPDAQVERINKFGAVYGSYMG

RTVYITISDPEMLREVFVKQFHKFTNRAPDGQSLNIKPSCRMMTQLVDEDWKNVRSTLSPAFSGGKLKHMSEAMNTCADV

LLKNLGKFAEKGESFDSKDLTSGFTTDVIARTAFGLEVDSQGNPEDPFVVYTRKPFAVAFRNPLFWLFFLFPKIMKPILE

FFEYSFLDKDVSSFFYNVVDQVMGMRQTEDKAHRHVDFMQLMMNAHKEDDDDDNKEEGTQVQGTKQPLTRDDIVANGFLF

FIAGYETTATTMGFLLYNLALHQEAQDKAREEINQVMEGRELVDYEAVNKMSYLEMCIHETLRMYSPASGVVRVVGDEVK

MKWLTLKKGMNVMIPILGIHNDPKRWPEPKKFIPERFTKEEREKRDQYDWLPFGAGPRNCIGMRLALMELKVGVARVLRK

YRIVPGPDTDVRSTIPRGVGPSPDML*

>fgenesh2_pg.scaffold_28000088|Brafl1

71% to estExt_GenewiseH_1.C_90137|Brafl1

MDLDFLPIPLTWVLLAALPVLFYLYAVRPMQALKKLGVSGPTPLPFIGNLHQTMKTGHYNMEAQVEYTAKYGKVYGVFNG

RTPILVISDPEMLREIFVKQFHKFTNRAPKGLSLDVTMQPRMLTQLVDDDWKNVRSTISPAFSGGKLKQMTEAMNSCADL

LVENIGKFGENGESFDTKELTGAFTTDVIARTAFGLEIDSQRNPEDPFVVYSKKALDFNFRNPLVWLFVLFPSIMKPILE

KVNYNFMANDVTDFFYNIVDQVMGMRRNEHGRVDFMQLMMNAHKDDDADMYNDATQDTKVQGTKKPLTKDDIVANSLLFF

LAGYETTATTMAFMLYNLALNQQTQDKAREEINQAMEDRDLVDYEAVHNMPYLDMCINETLRMYSPAANIARAAGEEVKL

KWLTIPKNMLIIIPTLGIHHDPERWPEPEKFIPERFTKEEREKRDPFDWQPFGAGPRNCIGMRLAMMEMKVGLARVLMKY

RFVTGPDTDIPLKIMKYKQFPTPENGIWLRAELLHPGSD*

>fgenesh2_pg.scaffold_143000060|Brafl1

98% to fgenesh2_pg.scaffold_28000088|Brafl1

MDLDFLPIPLTWVLLAALPVLFYLYAVRPMQALKKLGVSGPTPLPFIGNLHQTLKTGHYNMEAQVEYTAKYGKVYGVFNG

RTPILVISDPEMLREIFVKQFHKFTNRAPKGLSLDVTMQPRMLTQLVDDDWKNVRSTISPAFSGGKLKQRNPEDPFVVYS

KKALDFNFRNPLVWLFVLFPSIMKPILEKVNYNFMANDVTDFFYNIVDQVMGMRRNEHGRVDFMQLMMNAHKDDDADNDA

TQDTKVQGTKKPLTKDDIVANSLLFFLAGYETTATTMAFMLYNMALNQQTQDKAREEINQAMEDRDLVDYEAVHNMPYLD

MCINETLRMYSPAANIARAAGEEVKLKWLTIPKNMLIIIPTLGIHHDPERWPEPEKFIPERFTKEEREKRDPFDWQPFGA

GPRNCIGMRLAMMEMKVGLARVLMKYRFVTGPDTDIPLNIMKYKQFPTPENGIWLRAELLHPESD*

>estExt_fgenesh2_pg.C_720033|Brafl1 38% to CYP3A27

72% to estExt_GenewiseH_1.C_90137|Brafl1

MQALKKLGVSGPTPLPLIGNLHQTMKAGMYNMEAQVEAIAKYGTVYGVFNGRTPLLVISDPQMLREIFVKQFHKFTNRAP

EGMALDVKPQARMLTQLVDDDWKNVRSTISPAFSGGKLKQMTEAMNSCADLLVGNIGNFGEKGESFDTKELTGAFTTDVI

ARTAFGLEIDSQHNPEDPFVVYSKKAFNFNFLNPLIWLFFLFPSIMKPILEKFNYNFMANDVTDFFYNIVDQGRVDFMQL

MVNAHKDDDPDNDATQVPGTKKPLTKDDIVANSFLFFLAGYETTATTMAFTLYNLALHQEAQDKAREEINQVMEDRDLVD

YEAVHNMPYLDMCINETLRMYSPAANNGRVAGEDVKLKWLTIPKNMLVLIPILGIHHDPERWPEPDKFIPERFTKEEREK

RDPFDWQPFGAGPRNCIGMRLALMEMKVGLARVLMKYRIVTGPDTDGLKAF*

 

$$$$$$

 

>estExt_fgenesh2_pg.C_560111|Brafl1

35% to CYP3A4

MALEFCPVPLIWVLLPLLAVLFYVYAVQPLFVFKKMGVPGTGIPPLPIVGTSYTVLLSGIYNTQFTASDFGPVLGVFNGM

NPILIVSDPDMIKEIFVKQFHNFVNRQPEVQDINIMPXSRQVFQLRDEDWKNVRVTLTPAFSGSKLKQV

(gap) 36% to CYP3A4

VFPNIAK

PLFETFNYHPFTTRDVTDFFYGVMEQLLAMKKTDGGKGRVDLLQIMIDAHQESDDGSTSGPKVVGKRQPLTRDDIVGNGIG

FFSGAFETVSITMSFALYHLALDQEVQDRARQEIREVAGSEGEVGYEAVQKMSYLEMCIMETLRLYPLVSNIQRVANEEARLK

WITVPKGMRVMVPAIAIHYDPARWNEPRKFNPERFTKEEREKRNPMDWLPFGVGPRNCIGMRLALLELKVGLARALMKYR

FVTGPETR

 

PTRRDQVVCGCLLRSRKTLTGARTVKVQEYMEFGKEITVTHTEVVPVSAEVYWTGFDDYFHLQDFVGMHENM

TTIKGDGGVGTVIEFDFLGGRTQEELVQKDEDTKTWAIAMLGSNPLFTSYLATVKVDEDTSETAKVTMSIKGIVALQDYN

KRKDHIAFTKYMLRNRINEIMAVISEKKGDILPFEFDVDCSFKTLWDMVSDWANVSWVMGATDVEIVPDYQANDLRRKVF

FGSHFMEERHVQTIDHPASTTYEFGKNDTVGVLHYRGKLELIKLDETKTKVKYTSYFTPQPGADPKTTIKALMEPRFDWI

QATFAEKRSLFSSCCLL*

 

>estExt_gwp.C_560022|Brafl1 84% to estExt_fgenesh2_pg.C_560111|Brafl1

35% to CYP3A43

MMTLQEFLPVPLTWVLLPLLAVLFYMYAVHPLFVFRKMGVPGIGLPPLPIVGTSYLTILSGIYNTQFSASDFGPVLGVFN

GMNPMLVVTDPDMLKEIFVKQFQNFVNRQSEVQDINIMPQSRQLFQLRDEDWKNVRTQLTPAFSGSKLKQMIAALNSCAD

QLVENLNSFATTKESFDVKEVTGAFSMDVTARTIFGAEVNSQQDPQNPVIAHARRTFNFNFKSPVIWFYVIFPRIAKPIF

ETINYHPFTPRDVTDFFDGVMEQLLAMRKTDGSKGHVDLLQIMIDAHK DMATTKTANIKTQRKFLHLQYCRSKQHFPVISA LTRDDVVGNGIGFFSGAFETLSITMSFVLYQLALNQEIQDKARQEVNEVVGSEGEVGYEAVQNMSYVEMCVMETLRLYP

LASHIQRVAKEDARLMWINVPKGMNVIVPALAIHYDPKRWNEPRKFIPERFTKEEREKRNPMDWLPFGVGPRNCIGMRLA

LMEMKVGLARALMKYRFVTGPDTVSHM*

 

>estExt_fgenesh2_pg.C_540178|Brafl1

82% to estExt_fgenesh2_pg.C_560111|Brafl1

MMTLQEFLPIPLTWILLPLLAVLFYMYAVHPLFVFRKMGVPGIGLPPLPIVGTSYLTILSGIYNTQFSASDFGPVLGVFN

GMNPMLVVTDPDMLKEIFVKQFQNFVNRQSEVQDINIMPQSRQLFQLRDEDWKNMRTQLTPAFSGSKLKQMIAALNSCAD

QLVENLNSFATTKESFDVKEVTGAFSMDVTARTIFGAEVNSQQDPQNPVIAHARRTFNFNFKSPVIWFYVLFPRIAKPIF

ETINYHPFTPRDVTDFFDGVMEQLLAMRKTDGSKGHVDLLQIMIDAHK ESDDGSTDGPKAIGKKQP LTRDDVVGNGIGFF

SGAFETLSITMSFVLYQLALNQEIQDKARQEINEVVGSEGEVDYEAVQNMSYVEMCVMETLRLYPLASHIQRVAKEDARL

MWINVPKGMNVLVPALAIHYDPKRWNEPRKFIPERFTKEEREKRNPMDWLPFGVGPRNCIGMRLALMEMKVGLARVLMKY

RFVTGPDTVPMNLNRWRAFPTPMEPIRLMVEPVD*

 

>fgenesh2_pg.scaffold_54000181|Brafl1 35% to CYP3C1

83% to estExt_fgenesh2_pg.C_560111|Brafl1

MMALQEFLPVPLTWVLLPLLAVLFYVYAVHPLFVFKKMGVPGIGFPLPILGTSYVTAHMGVYNPEYSASDFGPVLGVFNG

MNPILIVTDAEMIKEILVKQFHNFVNRQPEVQDINIKPQSRQVFQLRDEDWKNVRVTLTPAFSGSKLKQMMAALNSCADQ

LVENLNSFVTTNESFDVK

(gap) 35% to CYP3A27

DIAKPIFETINYHPFTPRDVTDFFDGVMEQLLEMRKTDGGKGRVDLLQIMIDAHQESGDEST

NGPKVVGKKQPLTRDDVVGNGIGFFSAAFETLSTSMSFALYQLALDQEVQDKARQEINEVIGSDGEVHYEAVQKMSYLEM

CIMETLRLYPLGASILRVSNEEARLKWVTVPKGMSVVVPALAIHYDPARWNEPRKFIPERFTKEEREKRNPMDWLPFGVG

PRNCIGMRLALLEMKVGLARVLMKYRFVTGPDTVPVKLNKWKVLPVPMEPIPLRVEPLD*

 

>estExt_gwp.C_560025|Brafl1 36% to CYP3A4

81% to estExt_fgenesh2_pg.C_560111|Brafl1

MMAIQEFLPVPLTWFLLPLLAVLFYVYAVHPLFVFKKMGVPGIGFPLPIVGTSYQTAQMGVYNPEYSASDFGPVLGVFNG

MNPILIVTDAEMIKEILVKQFHNFVNRQPEVQDINIKPQSRQVFQLRDEDWRNVRVALTPAFSGSKLKQMMAALNSCADQ

LVENLNSFVTTKESFDVKEMTGAFSMDVTARTIFGAEVNSQRDPQNPVIVHAKRAFDFNFKSPLMFLYLMFPSIAKPIFE

TINYHPFTPRDVTDFFDGVMEQLLEMRKTDGGKGRVDLLQIMIDAHQESGDESTNGPKVVGKKQPLTRDDVVGNGIGFFS

AAFETLSTSMSFALYQLALNQEIQDKARQEINEVIGSEGEVDYEAVQKMSYLEMCIMETLRLYPLGASILRVSNEEARLK

WVTVPKGMSVVIPALAIHYDPARWNEPRKFIPERFTKEEREKRNPMDWLPFGVGPRNCIGMRLALLELKVGLARALMKYR

FVTGPDTVSHI*