ࡱ> >@=  bjbjв 28к_к_NN8,,X40hjjjjjj$zT   h h    G@ T0 I I  I4   INX : Batrachochytrium dendrobatidis P450s D. Nelson August 9, 2009 9 sequences >CYP51F1 Batrachochytrium dendrobatidis JEL423 (chytrid) 43% to CYP51F1 Rhizopus oryzae note this species has no obvious CYP61 sequence BDEG_01891 MGLFFEAYESVRNVSGLPPDQFHTVVAITSLVFVLTVWLLSYALNQPLKSKDTGSEPPRVPVSIPFIGAAIAYGVDPIKF LIECRQKYGDCFTFVMLGRKMTFCLGPDGNHYVFNVPVADATAEGAYKKLTVPVFGAGVVYDVHNAMFMEQKKFVKDAFN SNSFNAYMLNIWNETNAFFNDWEDHYDANMIFDEMSELTIRTASHCLLGKEIRQQLHSTVAQLYHDLDSGLAPINVFFRW LPLPVYFKRDRANKIMSKTFVDIIKKRRATNSTDNADLMQTLMFARYRDGTSMTDDEIAHLLIATLMGGQHTSSTTLCWM LFELARRQDIVDKLLHEQSMVLTGKPDTPPEDLPEFTHEQIRQLTFLDCVMKETLRLHPPIHTVMRKVEKSMTYRGMTIP AGHFICGSAAVSHLDPTRFPDPLKFEPSRFLNNDEGSGEWSINTIDIAQKSARSHFLPFGAGRHRCIGEAFAYIQNKTIL SAFIRRYTHKLYVDPNTGKEHFPSSDYTSLITIPEKPARLSITRRSVKA >CYP5224A1 Batrachochytrium dendrobatidis JEL423 (chytrid) only 25% to CYP617D6 Aspergillus niger 26% to CYP509A1 Cunninghamella elegans N-term part only, up to I-helix, added the rest from genomic seq BDEG_02920 Supercontig 3 1051607 - 1053730 (-) strand MNAVLGLISTGNGWAKQFESIVTSHSRSRYSLVCAVAIPAIGILILKRWLQPSLLPKGLP TPPYLPLVGQFFTILP (2) YANRRQTHEFFEKMRKTVGHIWLMKNMSTDIVVVSDPESVMRLLQNSTEITRGEKLQLSA QTMFEHSLFVLPGGPVWKKHRKLLQPAFGPLHLRFAAHASEEAIVQLSMLIEDEIRAADS ECYTIDIYQYMTAISMDIM (2) GQVTLKHDFKAVRSVYDQEDSK (0) TPGVFDKLVTFLQR (0) RLFIPPFLWGLVGLHNSSPEIRSTVGSVVN MVKAMIKDYKDAHKNDQSNDIKDKKHMDVLERLLHSNSEAQ (0) LFSEQEIIDELIGFIIAGHE (2) TTANTMTFIVFALCQNPKVQEKLAAEIHDIYNSLDEELNVDNILRFTYLD WVIKEAQRCYSVVQQVSRVSTQDIQLLGYTFKKGTRFLLRLADVHQDDRYWKDPKQFNPD RWAEPCTPGTFLPFGDGQFNCIGQKMALIEIRVIMIHLIRQFAFDLVPNQKLERFSTVTH GFKNGLKVKMTKRK* Wrong C-term part TTASTMTFIIFQLCIHPEIQTKLVTDICEIYSKLDKDIDMENIMHFEYLDWVIKEAQ RLDSVLQNVMRVSTNDIQLMGYEFKAGTQFMLRIADIHRDERYWKDPLRFNPDRWAE PCVPGTFLPFGDGAFKCIGQKMAMVEIKVVMIHLLREFQFELVPNQKLERFTSITHGFKKGLMVKISKRK* >CYP5224A2 Batrachochytrium dendrobatidis JEL423 (chytrid) 51% to CYP5224A1 added the N-term and C-term part BDEG_03897 revised (may have a gc boundary at SLDIM) MFTTIMSVIPFTMPSISLYFGFMEKKYCPSVYLIAP VLVCFGLACWRHLSKPLNGLPGPYAWPIVGQFPILLM (2) FLKNHQTHLYLAAMRKKYGPIWILPSLGIDSVVVCDAKAVQSIFQNSTDIIRPDSGFRAAKSL FQYALFILPGGPLWKKHRKLLQSSFSLPHLRLAVHASKETAAVISTVINNFI QADSSKSCIIDIHCYMISLSLDIM (2) GKVAFKYEFNAVKQTVSGNNEK (0) GYKLFEKLLVLLQK (0) RVLLPKIFWSYANIADNSPALRETADYMRLTVKKVIDERDASCESELSKDPLHMDVLERLLNSRTGDL (0) VLSEEEIISEIIGFIIAGYD () TTASTMTFIIFQLCIHPEIQTKLVTDICEIYSKLDKDIDMENIMHFEYLDWVIKEAQRLDSVLQ NVMRVSTNDIQLMGYEFKAGTQFMLRIADIHRDERYWKDPLRFNPDRWAEPCVPGTFLPFGD GAFKCIGQKMAMVEIKVVMIHLLREFQFELVPNQKLERFTSITHGFKKGLMVKISKRK >CYP5225A1 Batrachochytrium dendrobatidis JEL423 (chytrid) frameshift at &, first exon is a guess. BDEG_08295 MLSNPLLYSKISQIRPCYSIAVVAYSRKATTVSMPITREDTVLSSRWG (0) DPIGFLNKQREIYGDAFTLNLVNTKMHFFISPAVCTFMIESSI (2) SLFQMQKFSLDQAFYDRCPPFKIQGMTDKYSKLFLKLTATALNQPKMLHAYHPLMTDVVSATIMEFTQSGTLDVFELTSSIAVK IAIVWILGKQFYKAHADDMVQLILQIEKDIASPLNSFFPGWPHPPCIRMQKAIETMFKWVSHEIKLRRMVNANAPVQCDL LQLLLNCHECNVLLEADISRFMLTLFLAAHTNTAANMGWTIYELAKHTHLQQ (1) HSDSTFTDFYPSS GTRNLESHMPITTACI & KETGRLYMKLFTFKEIVVRCSVWWI GYKILKGELICISPGCVALDSKLFEYPNVYNPDRWLP NNHKSTTSKPKIIPPMNMPQFGIGKHHCLGERVVLMAIKTIVLPELLRRFECTLDSNEQI VIPDYFGSISSPFPKHPIRIKVVPI* >CYP5226A1 Batrachochytrium dendrobatidis JEL423 26% to CYP5224A1, heme signature is not correct, no CYS in cyan region BDEG_02487 MPNVDFRYKNNQQFHKLQTWLTTNYGQITMLYFYSKRIVFVSSPLYAKQILSSSLFVQDEESVKLFEGIVDIPSPEDRKR HLSTVKQLSMELLHARILDITMERTFRVLISWNEKIRIDSERLVVDLDNVCKAISLDICGLGILSMDFDAVTGLRIKQIT LSEIVLNKVVRFLHQRANLAPFAWAYNKVGITSSDLVSARTHFKSVLKSLIREQCHPYVDDPLSSVNIIRTLLKDYGTDL TPENEQAIGADVYRGMLWSNMFLSSALSFVFFELLQNDDVREKATQEIDRVWKATHGKIQLDTLSQLLYVECVVKESLRL HPVCAYLVKESKESVKVGNYLFPTATQFRISVIDLHQDPLIWNDPSAFLPQRWEKTDANTLPFYPFGDDPDLVVQKLALV PIKAAVAVFLRHISFEHAKKDTADEVPSATHDIDASGAVCKLYDENSLLECIATQRPLDSLQ >CYP5227A1 Batrachochytrium dendrobatidis JEL423 35% to CYP5224A1 Batrachochytrium dendrobatidis BDEG_02013 MDTTVASASIVTLAAGLAIVSYLQNYYVRIPGPYAYPIIGSGLEVAKYSKEGNYHLFLAWMRTFGPITRINIFMKNVVVT TDAALIKRATTSSTEFWRDPAFSKIAHSLLDNGLFTLPTGDVWKRHRKNLQPAFAPSHLRQAAKITRERIETLGDYWQQE AVNNGGSIIVDIFHEFTALTLDIIGSAIFSYDFHASDDLHKKRDQVSQGMVEELVRLVQERFSVPPLLWAFNNLSSYSSR VKKVNGCIHAVIQDVIDKRKEQMKLGNIPSNDQMDVLDRLLKVHQVAAENFTNEEVVGEVIGFFVAGHETTANTLVLTAL EICRHPEIEQRLAKEVAQVEKSQGCPINIESVPSLKYLDMVVKESQRFHPVVSNMGRNTLCTIQHNGYTIPANTTLILNT GGAQKDPAYWDKPEKFDPERWADGFVPIPGSYVPFGDGPMACIGQKMALIEIKIVLSHVLSKFDMELIPDQDLTLVTSVT TGLKHGLKLRLTPKTVQ >CYP5227A2 Batrachochytrium dendrobatidis JEL423 59% to CYP5227A1 Batrachochytrium dendrobatidis 35% to CYP5224A1 Batrachochytrium BDEG_02014 MSNNSVIAGTLVAAIAAMTYLQNNYLGFPGPFAYPIVGNGLDFVHQTNKGKYQDYICSLQSYGRVVRINVFGANYICTTD AALIKRVLTSGTEFWRDPTFANISHSLLDLSLFTIPSGDVWKRHRKNLQPAFAPTHLRQAGNITQNQADKLVRYYNSESL KNDGSITVDIYHEFTALSLDIIGQVIFSYDFHACNDLHAHKVQVGQGYIEELVMFIHQRFTLPSILWGMAGLSSNSTRVK LASTYMKKLIQNVIDKKKSELKQNDEKDPKCMDVLDRLLKVGPSESENFTDEEVVGEVVGFFIAGHETTANTLVNTTLEL CRNPEITRKILAEIHQVESTLGHPINTESLSSLKYLEMVIKESLRMYAVVASTYRTTLCRIEHEGNVIPSNTKFIVNIGG VHHEPVYWDKPEKFDPDRWADGFVPIPGSYLPFGDGPMACIGQKMAMIEIKLALCALLSSFDMELVPNQDIVFVTSITTG LKHGLKVKLTPRSAASV >CYP5228A1 Batrachochytrium dendrobatidis JEL423 32% to CYP534D1 Mgr039 Mycosphaerella graminicola BDEG_02899 MSASLTKWLRISLVSLPALYTIYTVIRWRLLAAKERRILERLCPTLPVITINFNRTMFGIIDVTHFSIIPTWWMPKILPK GWQWMGGHDVYQQLGEDTIAIVSPGDRQFWVVDADLVKDIVSRKSDFVKPVHIYTILNIFGSNVVTTEHAEWRRHRRIVS PQFSERINLSVLQYAAETVRDMLKCWQSTEHTAHGEVRVNVSNDMMKLALHVISGAGFGMRLDWVTSESDQTLDHGHTMS YKTAVHSMMAGLLLKSILPKFAYLLPIERVQNVKRSFDEFGLYLSEVIRGADKQDESNLLVSLAKAARAEDGGLTEQELV GNVFVFLFAGHETTASTFMFALAMLASHQDVQQRLYEEIQLILDGNDPQLKDIQNLGYTLAVMNETLRMFPPVVAIPKHT TSQQTLGKYIIPEDTRVTLHTVGIHYNPKYWGPDPSVFRPARWFTLPECASSAVKTRDFIRSTTQLKSQTSPSEPEHDKT RSDVPDTASDKPHQSLFSYNRYAFLPYSEGMRSCLGKRFAQVEFITGLAMMMQKYSVHLPDGAQIEEVIESQNRVTLQPK RDMYLVFKPRTST >CYP5229A1 Batrachochytrium dendrobatidis JEL423 (chytrid) 23% to CYP5225A1 no heme signature. 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