Candida guillermondii P450s

 

D. Nelson

 

August 9, 2009

 

10 sequences

 

>CYP51F frag. Candida guilliermondii X97680

this seq is 94% to Candida albicans but only 66% to

full length CYP51A1 for C. guilliermondii

This is a different gene

MTDQEIANLLIVILMGGQHTS--TSAWFLLHLGEKPHLQDVIYQEV

VELLKEKGGDLNDLTYEDLQKLPSVNNTIKETLRMHMPLHSTFRKVTNPLRSPNKYMS

SHTVYMFKFL

 

>CYP51F1 Candida guilliermondii

71% to CYP51F1   Candida lusitaniae

PGUG_03415.1

MAIADIATWVYSEFRGLPVSAQISIVVLFPFVYNLLCQFWYSLQKDRAPLVFHWVPWLGSSVVYSMQPYEFFESCRQKYG

DVFSFVMVGRVMTVYLGPKGHEFVFNSKATDVSAEDAYTHLTTPVFGKGVIYDCPNSRLMEQKKFAKFALTKDSFIKYVP

KIQDEVLSYFVEHDFKMKEKDSGVVNVMKTQPEITIFTASRSLMGDEMRKRFDTSFAQLYSDLDKGFHPIHFAFPHLPLP

MSRNRDRAQQKISATYKGLIDTRRSSGNIDPTRDLIDSLMATSTYKDGVKMTDQEIANLLIGILMGGQHTSAATSAWFLL

HLGERPDLQEEMYKEICGVFEKKNKSFKDLSYDDLQEMPLVNNVIKETLRMHMPLHSIFRKVKKPLLVPGTKYTVPKGHY

VMVSPGYAMVNDRWFPDAASFNPRRWDEQPLPTDGQDETVDYGFGNISKGVSSPYLPFGGGRHRCIGEQFAYVQLGTILA

SYVYNVTWELKDKLPGVDYASMVTLPLEPADIVWKKRRTCVF

 

>CYP52A39 Candida guilliermondii

66% to CYP52A14   Candida tropicalis

PGUG_01238.1

MFDVDTVHNFFTSWYGILLAVLIGYHVFDYIRIQIVMKKLGCVSPPVESDGFFGFKLLYTSLKHKKEGTLVNFIKERFET

VGKDTFSFRIAGTPVISTKNPENIKALLATQFSDFALGTRHAQFKPLLGDGIFTLDGSGWKHSRAMLRPQFAREQVAHVK

SLEPHIQMLAKHVRRAKGGAFDVQSLFFRLTVDSATEFLFGESVESLQDESIGMAKDAVDFDGKAGFAEAFNTAQVYLSI

RSLAQKAYFLVNNKEFRSSNEKVHKFADYYVQKALNSSPEELEKHSQDGYIFLYELVKQTRDPHVLRDQLLNILLAGRDT

TAGLLSFTFYELARNPQVWSKLKEEIYEKFGKGDDARLEDITFESLKKCEYLKALLNEVLRLYPSVPQNFRVAQKDTSLP

RGGGPNRDQPIFIAKGQTVTYTVYAMHRDEQFYGKDSEVFRPERWFEPETRKLGWAFLPFNGGPRICLGQQFALTEASYV

IARLAQLFPNLASHDDEYPPRKASHLTMCHQSEVKISLA

 

>CYP52A40 Candida guilliermondii

60% to CYP52A25   Candida dublinensis

PGUG_05855.1

MSELLDSIQPYLTKWYVVISALLVSFFIAHKISVARFKATHNCAASPEYYKVNWFSLPLLYRLIQVKREGRLLDFAQKIY

DDVKALTFVIKIVGVPVIITRDPENMKAVLATQFNDFALGTRHAHFKPLLGDGIFTLDGNGWKQSRSMLRPQFSREQVAH

VQALEPHLQRLAKHIRLADGETINIQDLFFKLTVDTATEFLFGQSVYSLKDAAINDPPTEDFDGRSSFANSFNTAQTYLG

TRAYLQMFYFIVNNSDFRKCCNQVHDFTRFYVQKGLDMTPEELEKKSENGYVFLYELVKQTRDPKVLQDQLLNILLAGRD

TTAGLLSFTFFELARHPRVFNKLKEEIYEAFGKGDDARVSEITFESLKKCEYLKWVMNEMLRLYPSVPVNFRVATKRTTL

PRGGGPDGNSPIYVGKGTTVAYSVYSTHRMEEYYGKDADEFKPERWAESRKLGWAYVPFNGGPRICLGQQFALTEASYIV

TRLLQMFDKLELHDDRPYPPAKSVHLTMCHQDGVYVSLS

 

>CYP52A41 Candida guilliermondii

52% to CYP52A12   Candida tropicalis

PGUG_04005.1

MLSATYVILTLVAFFTVRQVIITIKRRKFAREHGCQNFTKTVGMVSVIDNIKKVYKARREGRLTEQLQESYDLYGVDTFQ

ARAIGTSAVFTRHPENIKAMIATQFDDFDIGKRQQVFRPLLGFGIFAAEGQRWKHSRAMLRPQFAREQVAHVQSLEVHLQ

NFVKLIKEQNGSFFDIQHLFHRLTMDASTEFLFGESVGSLKSPNNEFEAAFNTSQHYLTMRATLRGAYFLVNPKEFRDAN

EVIFKTVRYYVNKALNTSPQELAKQTEEGYTFLYQLVNQTRDPQVLQDEMLSIMLAGRNTTASLLSFLFLELSRNKHIWK

KLREEVHEHFGNGENARLEDITFESMKRCNYLKWVINETLRMYPSVPTNFRHAAKDTTLPRGGGPTGDSPVFIKKNTDVY

YTIYSTHRMKEYYGKDAEEFRPERWADLKNIGWAYMPFSSGPRICLGQQFALTEASYITIRLAQIFTELDTSDHAYPPLK

IANATMRPMNGVNVRFQ

 

>CYP52A42 Candida guilliermondii

62% to CYP52A23   Candida albicans

PGUG_05670.1

MNGFEEVHNFLTSWYGIVLAAIVAYNVFDYVRVKVVMKKLNCVGPTVESDGFFGFRVIFTLLKHKREGTLVDFRKDMELD

TLRFRVAGTPMVITRNPENIKAILATQFNDFVLGGRHDHLGPLLGDGIFTLDGSGWKHSRAMLRPQFAREQVAHVKSLEP

HIQLLAKHIRKFQGEPFDIQKLFFRLTMDSATEFLFGESVDSLHDETIGFSKSAIDFDGKNEFAEAFNTSQIYLSIRALA

QQVYFLINNKEFRDANAKVHKFADYYVQKALSFSTEELDKYSQDGYIFLYELAKQTRDPKVLRDQLLNILLAGRDTTAGL

LSFTFFELARNPDIWSKLKEEIYNRFGSGDDSNLEDITFESLKKCEYLKALLNEALRMYPSVPQNFRVAQKDTTLPTGGG

PNRDLPVFIRKGQTVAYMVYALQRDQRYYGKDSEVFRPERWFEPETRKLGWAFLPFNGGPRICLGQQFALTEASYVIARL

AQLFPNLASFDDEYPPRKASHLTMCHQNGVVISMT

 

>CYP61A1 Candida guilliermondii

79% to CYP61A1   Candida dublinensis

PGUG_05085.1

MDVNSTNQVTDMYSTPIGGTVGSYVNDVFTNLTWVQIFCTTVVLIFTYDQVMYQLRKGSIAGPRVKMWPIIGPFLESLDP

KFEEYMAKWNSGPLSCVSIFHKFVVIASDRDLTRKILASPKYVKPCVVDVAVKILRPSNWVFLDGKAHSDYRRSLNGLFR

SKALEIYIPVQEKYMDKYLEKFTNFDGPRKFFPEFRELLCALSLRTFCGDYITEEQIKLIADNYYRITAALELVNFPIII

PYTKTWYGKMIADDTMKIFESCAAMAKKHINEENGTPTCIMDEWISLMKDAREKHSEDAASKLLIREFSNREISEAIFTF

LFASQDASSSLACWLFQIVADRPDVVKKIREEQLKVRDNDPSKPLSYDLINEMTYTNFVVRESLRYRPPVLMVPYVVKQK

FPVTETYSAPKGSMLIPTLYPALHDPEVYDDPDSFIPERWYDASKEMTNRNWLVFGTGPHVCLGQNYVLMLFTGMLGKYM

MNADLIHHKTPLSEEIKVFATIFPKDDLIMEWHSRDPSKL

 

>CYP501C1 Candida guilliermondii

43% to CYP501A2, yellow is long

PGUG_01534.1

MSSLWYLVTDCILLFIFAWIIEDCILALGVEPPIPGIKQIPSYPIVGNLLQVKNNPAKVYLKWSKFYKVPVFQVRLGFRN

IVVANSYQSVEFLWGKSASANNSRPTSHIFHNIVSQSQGFTIGSTPASSSLFKTKQALSSELNRKSVNMIAEQLDQQVKY

TLRMIIRNNVELK

GSPSTFLSHVRSRLS

DIDLLPYFQLFALRSAIQLTYGITLDCYGKHGRISQEIIEVENQIIKLRGQG

SWLPDFLPFLKYFGNWELSASKYRLRRDNYMNELSRDFKKRYSNNDAAARTSLLGRLYKNSSHLAHHELNSICLTMVSAG

LDNTPLTINHLMGHLSRPDYGYALQQVAFSQLLNCYGDINDAWNNCGTNMECNFVVALVQEALRFFTVLPLSLPRKTTKV

IKFNGVVIPEGAVLYMNAYAANHDSEKFSNPMKFDPFRWLVPRTMKLKDRREMDHFAFGAGSRKCSGNILATQEMYSMIC

RLILIFRIRRPIEEAMELDPFESNACPSATSFEPKRFKVHLEPRCHEGSEVLYRSLQ

 

>CYP504G1 Candida guilliermondii

PGUG_05169.1

38-39% to many CYP504 sequences, also 39% to CYP5054A1 and 41% to CYP5054A2

MLLDLLFDFLNSYGYLLLVVSILIAAKSVFGAAYCTGIRKIKGIVEPPECEAFYGHLNVLGEDHATNLQTIGDRYNLKVF

QVRFGVERAVILHTFAEGFSWFVKNHQSALIDRPLFYTFHKLVSNTQGLTVGTSPWDENCKRRRLAITKYMTRTALQKIQ

DLIDLESCSFVKDIWRLTCEGSIGVDPHIAAQRMSFNITTMLCYAIRFDTIDSSLLLDILKIVSQVSSFRSTNNNLQDYV

PIYRYLPDKRREMAKEVSKKRDVWLNDFLNRAFERSEVRESFAGSMGTKKLSLEDLRSICVSLVSGGFETLASITSLLLA

QLSTENGKEWQETAYGELTDAYGNDGDKLWNSVTFEEKCPFVVALIRETLRHYPVIPLCPPRKTTREFEYEGAKIPKGVT

VILNCQAANHDKEHFGADADEFNPARWFDESLDINKPPFHFTFGAGSRACPGILLSHRALYATIGRMIFLFKIGPHGEKP

DLNYKTYAEDKTAQTWSPLPFKISLEPRGPVDKFLNRDHLQHLVELSYEHI

 

>CYP5217A4 Candida guilliermondii

51% to CYP5217A2 Candida lusitaniae, yellow seems too long

54% to CYP5217A1  Candida dublinensis with yellow removed

PGUG_02308.1

MLLDFFSFLANAAQTHTSLVIWSLCGVTIIYYLIIYPFFLSPLRNIPGPYHYRVTKLFALNGQRRQQWIMTVHQLHQKYG

NVVVLSPREISVNGDLVYLRDIYTKNFPKDRFYENFRNHGFKDNIFASLENDRHKNYKKILQGLYTKSAILSDRNSTKDL

LNDKVNLLMDQIYNSS

VTAKNPDLINAKCLHNEHGVGVASTWYEKTKQK

GIEVYSLFGALAMDVVSGFELGPENGTSLLS

TPKDRYIIAMFRRVASMGFWTTLMPQLWNFAATGTILAAQQAVEKWQLSAYTKAEKNVPKVTTSLNTLNAAGLFKESAYS

FLTDNIFAGHETTAIQLTYLTYELSRPVHRENQHKFRQELYNTFGKPHDINDRITDLDKVDKLVYLDALMNENSRVHTSI

PGAEPRVTPEPYPINLEDGTKVIVPKGTTISVQPYSIHRQESVFPEPEKWVPERWLQQPNESIEEFHCRIKAQHKWMMPF

GKGIRMCLGMNLAVLEMKLALANIYWHFESKICEDWCEIDCNDNHIKMDSEPKNRKKDYEMMTMMDTYTTRPLYDECWLS

WSIPENS