Candida lusitaniae P450s

(Teleomorph = Clavispora lusitaniae)

 

D. Nelson

 

August 9, 2009

 

8 sequences

 

>CYP51F1 Candida lusitaniae

72% to CYP51F1   Candida tropicalis

CLUG_04932.1

MALAEYVAWGYAQFCSLGLLSKIALLVFTPIVFNVLWQFVYSLRKDRVPMVFHWIPWFGSAIPYGMQPYEFFESCREKYG

DVFAFMLLGRVMTVYLGPKGHEFVLNSKLADVSAEEAYSHLTTPVFGKGVIYDCPNHRLMEQKKFAKFALTKDSFQKYVP

RIQEEILDYFKSCSDFSMTERNSGVANVMKTQPELTILTASKSLMGDDMRARFDTSFAQLYSDLDKGFTPINFVFPHLPL

PAYRRRDEAQRKISATYMSLINDRRKSGDIVPDRDLIDSLMLNSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWFL

LHLAEKPELQEELYNEVLSVLTKKGGKITDLSYEDLQEMHLVNQTIKETLRLHMPLHSIFRKVKHNLVVPNTSYVVPKGH

HVLVSPGYAQTSERWFPKADTFDPHRWDNDWASSVSEGDAVDYGFGKITKGVSSPYLPFGGGRHRCIGEQFAYVQLGTIL

ATYVYNIKWHYAKGHSLPDVDYQSMVTLPMAPAEIVWEKRETCVI

 

>CYP52A29 Candida lusitaniae

55% to CYP52A13   Candida tropicalis

CLUG_03984.1

MSLHGVFGGMSIWSSVLLFIGIYLAGSYIRRYIAYKRSGAMWPAYGTDKCLGFKVVREMLKMRQIGRAPDFFQSKFEITG

VYTGGLLVGGRYAITTRDPENVKAILATQFNDFNLGNRNQHIKVTLGDGIFTLDGAGWKSSRAMLRPQFAREQIAHVQSL

ERHVQRLAQKVRGTKGERFDIQPLFAKLTIDSGTEFLFGESCESLVDGESESETGLDPALKAVFAEAFNYTQTILFQRLS

LQKLYFLVDGFKFRRQNRIVHQVTDFFVERALHASDKEAHSSSRGGYVFLYELMKQTRDPKVLRDQCLNVLLAARDTTSG

LLSFCFFELARNPAVFARLREEISFHFGLGESADLSAISFESLKKCEYLRAVLHETLRMYPSVPANFRVATRDTTLPHGG

GPDESKPIFVPKGTMCIYQVYSVHRSEVHYGKDYNDFRPERWFEERTKKLGWAFLPFNGGPRICLGQQFALTEASYVIVR

LLQMFEHIESFDSVYPPAKATHLTMSLFDGANIALW

 

>CYP52A30 Candida lusitaniae

55% to CYP52A23   Candida albicans

CLUG_04851.1

MLLSELKTAWDSFSSHLSDNVFLYYAELLILTLVVYHTYRTSKKWYLRKKLGAESAKWLQTSSAGWINSLPMREILDAKR

EGYLLRELWKYWHDDDENFMVKVAGTEMFVTTDPENYKALLATQFNDFKLGIRHSHFKPLLGDGIFTLDYGGWKHSRALL

RPNFSREKVGHLHSLETHLQVLFKHIRKHNGKPFDLQELFFRLTVDTATEFLFGHSANTLEDESIGIHKEETFEGEKEFY

DCFNISQAICATRAWTQKGYFLTIPFLYKEFTRCNRVVHNFADYYVNMALNMSQDELEKESNDGYIFLYELAKETRDPKV

LRDQLLNIMIAGRDTTAGLMSFIFFELAKRPDVWAKLKAEIYERFGYGDNVRFDEITFETLKKCTYLKNVVNETLRLYPS

VPVNYRQANINTTLPRGGGPDGDKPVLIEKGNAVGYIVSATHRNEKYYGKDAAEFKPERWDDKNLKPGWAYLPFNGGPRI

CLGQQFAITEASYVVTRMIQEFPNIADHDVEPDTYPPRMISQLTNSLAAGCYVSLTK

 

>CYP52A31 Candida lusitaniae

52% to CYP52A18   Candida tropicalis

CLUG_04098.1

MSLFRELDPVQLVYVVISLFFASFVYNELKGIYLSRKWHTKSPVKTYRGLFSLPQLVRSRKAVDSGHFVDYFRDQYIKYG

ADTLDFTGFGSKIFFTKNPHNVKSLFATNFDDWSIGARKRAFKPFLGVGVFVTDGEVWKHSRTMLKPQFSREQIAHVHSV

ESHYKLFAQHIRSYRGQEFDIQPLFMKLTMDASSEFLFGESIHLLHDDSIEHEKDTSDIKGKKELAEDLSFLQTYLMFRV

MLLDWFWLENSKKFSEAIKRVHAFTSQFVDRVLSLTPEEREANSKDGYTFLYELAKVTNDPIVMRDQILNVMLAGRSTTA

SLLCSVLLELSRNPEVYEKLKNEVYEQFGNGKTKGEEITFETLKKCTYLRWVLNEGLRMYPPVPSNLRQAVRDTTLPKGG

GPDGESPVLVRKGQYVALHIYSMHRSEEFYGKDALSFRPERWADLSKIGWAFMPFGSGPRICLGQQFALTEASYILVRMV

QDFPHIASHNKTYPPRTYSRSTMHYIDGIRISLY

 

>CYP56D1 Clavispora lusitaniae ATCC 42720 = Candida lusitaniae

AAFT01000066.1, CLUG_02306.1

46% to CYP56A1

MLIDIIISNISVSFWCLCFFFLSWKIFRIIYPPFDFPKNIPTIPFYAAFVSSFLGWDQNK

VYAHYYRENLEKYGAAKIYFASRWNILVTKPEYLAQIFKQNEIFEKSGNQHKIPYSLLAE

YTGDNIISTGNKYWKLYRKAVTNSIQFPNLEPLQTNATVLIENLSAASDNGYFPIADILQ

AYTLNNVGDCVIGTNFHKSDNFDISTSLKKVKQEIFRPLFLNFPLLDLLPFPSRIRARKL

VRSFKRNYCNKILNELNEENKGRLGHNLATFWKQGDITEKQFQDNAMIAMIAGHENPQLL

LTSLIYVIAKYPEIQRQLRSEMNSSNYDVKEQIPLLKAVVYETIRLFPPLGLIVNRKTRK

NVILGNGIFIPKGTYVGYNNFGTQRDRNYWGDDADAFRPERWGNDGKKIEEKYAEAKSKC

TLPAFHGRSRACLGEKFALAEVRKAVIEILQNFEISLDPSWKEQLTPAGPICPLKLSVML

KKVCSEHEHNI*

 

>CYP61A1 Candida lusitaniae

78% to CYP61A2   C.albicans

CLUG_04246.1

MSLNSSSPIVDAVSSKALETSSIVARIAETWHSLSWLQIFITFVIGVLSYDQISYQIKKGSIAGPRFKVWPIIGPFLESL

DPKFEEYKAKWDSGPLSCVSIFHKFVVIASTRELARKILSSPKYVKPCVVDVAVKILRPTNWVFLDGKAHVDYRKSLNGL

FSLKALEIYIPVLETYMDKYLDKFVTYDAPRKFFPEFRELLCALSLRTFCGDYITEDQIKEIADNYFRITAALELVNFPI

IIPYTKTWYGKKIADETMKVFEDCAAEAKIHINEKNGKPTCVMDEWIFLMKKARESHAEDSESKLLIREFSNKEISEAIF

TFLFASQDASSSLACWLFQLVADRPDVAAKVREEQMRIRNNDPSVPLSLDLINEMTYTNHVVKEALRYRPPVLMVPYVVK

QPFPVTPEYTAPKGSMVIPTLYPALHDPEVYPDPDSFIPERWENATGDMNKRSWLVFGSGPHVCLGQQYVMMLFTGMLGK

YLMNSEIKHHVTPLSEEIKVFATIFPKDDLILEWEKRDPLSATS

 

>CYP501B1 Candida lusitaniae

38% to CYP501A3 with some indels removed

CLUG_05046.1

MWLALLLLVALLFFLPPPRPIVGAFYVPGYPLVGNACQVLHNPSRVFLSWAQTCGHALFVIHLGRTPVLVVNSHADVHDL

WLLHSSALGSRPTLHTFHNVVSAVHGLTVGSTPAGASYRRKKKTLSSELSASRVQSKV

LSGNAQRALLSPNLSSVPKSSSIDSGHESGFPPILSHIDVCSSECIAQLLSHPA

ETSLLLETQYFVLSAAIRLTYGIVLDCHGRDASLANTIIATENKIIRM

RSLVANYQDYIPGLQSFPLRCMFDARAAKWRQQRDSYMHSLYQSFLVQLHGGSFLANHSMLAHILRAKASPMTLSESELQ

SICLTMVSAGLDNSALTFDHLMGHFSHDYGQDMQERLWKELMLQSNGSLATAWDAATYGTCSYAMALLHEALRFFSVLPL

GLPRQTTKPVRFRGLYIPANTIVVMNNYAANHDPAAFESPFEFSPDRWLDP

NGNLITPNHFSFGAGSRKCAGSHLALKEL

HTLLCRMVLLFRIKPPQDARFKMCLDPFEGNSCPTGTSFEPKEFRVRLEPREGKECDELRDRLRDYRIPQERAK

 

>CYP5217A2  Candida lusitaniae

CLUG_02702.1

50% to CYP5217A1  Candida albicans (probable ortholog)

yellow part is too long, may be an intron seq

VTRSSVSGKEPDYINARSASNHNGKGYALGSLDWFNKK this may be an intron seq (remove)

MKTLNINRSTHYTVSRNLQSSSSDARPIGKWSIALKCACISRFRAQKSGIARPKRKIAELPISRYSVPNLPCLSAIQKML

QDAAIVTARYVLENKNAFLGAIILSLALFKLVIYPFYLSPLKDIPGPYWHRVSHLPALEGQRRHVWIKKVHDLHAQYGKV

VLLSPTAVSFNEDPKYVHDIYVKNMPKAQFYANFTNHGPRPNMFASLDNKAHLRFRRMIQNLYSKSAIFNPKNSTRNSVV

EKVGQLVSQ (0)

GKSSNLGIDVHSLFGAFAMDNISAFELGPENGT

KMLLNPEDRYYLIPFRLIVDMTFWTTLMPRFWDIAAGPVIRKASRLIEKWQLDLYAKAEENVPQRAPGQNLSTLETLKKH

GLQGKNAYSSLTDNIFAGHETTAIQLTYTCYELSRPANKHRQEQLQKELREHFGSPNGDSEVIDDLEAVDRLPYMEALMQ

ETSRLHAAIPGAEPRVTDKNYEVVVGEKKVSLPVGTEVSMQPYSMHRVTSVFPDPELYLPERWLQEPGESEDDYAARMTN

MQRYMMGFGKGVRMCLGMNLARIEMKMALANLYWRYSSDICRDWCTVNEESEKATALNGAKAGLPVGLHSAVSPADSDEK

MMSMFDAYTTRPLHDECWLEWSVY