Candida parpsilosis P450s

 

D. Nelson

 

August 9, 2009

 

14 sequences

 

>CYP51F1 Candida parapsilosis

75% to CYP51F1   Candida tropicalis

AF019902, CPAG_03310

MALVDLALHGYNYFMTLSTLQQFGLLVFAPFIYNIIWQLLYSLRKDRVPLVFYWIPWVGSAVSYGQDPYGFFEQCREKYG

DLFSFVMLGRVMTVYLGPKGHEFVFNAKLSDVSAEDAYQHLTTPVFGKGVIFDCPNARLMEQKKFAKTALTTDSFRRYVP

LIRGEILDYFTKSKVFNMKKQKSGVVDVLQSQPEITIFTASRSLLGEAMRKRFDASFAQLYADLDKGFTPINFVFPHLPL

PHYWKRDAAQQKISETYMTEIARRRETGDIDENRDLIDSLLVNSTYKDGVKMTDQEIANLLIGVLMGGQHTSATTSAWFL

LHLAEKPQLQDELYQEVLNALSGKGGNLDDLSYEDLQQMPLVNNTIKETLRLHMPLHSIFRKVVSPLVVPNTKYIVPRGH

HVLVSPGYAHTNERFYKDASDFNPHRWDESASTNDAGEVDYGFGKVSKGVSSSYLPFGGGRHRCIGEQFAYVQLGTILTT

FVYNLKWKLANGKVPDVDYTSMVTLPQHPAEIVWEKRDTCVI

 

>CYP52A32 Candida parapsilosis

71% to CYP52A5   Candida maltosa

CPAG_03577

MSDLTLFDYATKWYVLAPALIVLYAVYIQVYNAYLIKKLGAAKEANCEGDGLFGFRLPFWLIERKENGTVVDYVAERFDE

VSHPSIPTFSIRIFMVKLWMTKDPENIKALLATQFNDFCLGTRHAQFKPLLGDGIFTLDGQGWKESRQMLRPQFARDQIS

HVKMLEPHIQVLFKQIHKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASIGMQAVSNEVEGKEQFAKSFNFSQNY

LASRTTMQGLYWLLNSKKFRDNNAVVHKFAQYYVKRALSLTPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRD

TTAGLLSFVFFELARSPAVLTKLREEIGAKFGLDKEARVDEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATKNTTL

PRGGGPDGMSPIFVTKGQIVQYCVYATHRMEEFYGKNANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASY

VTTRLVQEFSSLWMDPNTQYPPHKMSHLTMSLYDGCNVKME

 

>CYP52A33 Candida parapsilosis

73% to CYP52A5   Candida maltosa

CPAG_04394

MSDFDIMEYSTKWYIIAPALIVFYVVYKKINEAYLMKKLGAVRETNPEGDGYLGFKLPFWLIERKKNGTLVDFSEQRFHE

ISHPEVPTLSMRIFTVKLWATKDPENIKALLATQFNDFSLGTRHPQFKPLLGDGIFTLDNQGWKDSRQMLRPQFAREQIS

HVKMLEPHIQVLFKHVRKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASVGMQADSNEIDGKEQFAESFNFSQNY

LASRAVMQGLYWLLNSKKFRDSNAVVHRFAQHYVKRALSLSPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRD

TTAGLLSFVFFELARNPAVLAKLREEIGTKFGLGKEARIEEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATRNTTL

PRGGGPDGMSPILITKGQTVNYSVYATHRSEEYYGKDANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASY

VTTRLVQEFSSLWMDPNTQYPPHKMSHLTMSLYDGCNVKME


>CYP52A34 Candida parapsilosis

71% to CYP52A5   Candida maltosa

CPAG_04395

MRGQCGLPFFFRKNTCNSTITLESCNGYLTRATLATDIKRWAILFSCSTSIQTCLFFNYYNEVSMLDFNIMGYTISLFVL

APALIVLYVVYKIITEVYLMKKLGASRETNVVSDGAFSLKLAFTLIASKNNGTLIDYVAEMYRGAPHPEVPTFSSRIFTV

KFYLTKDPENIKALLATQFNDFCLGTRHAQFQALLGDGIFTLDGKGWKDSRQMLRPQFAREQISHVKMLEPHIQVLFKHV

RKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASVGMQADSNEFDGKEQFPQSFNFSQSYLASRVVISFMYWVLNS

KQFRDSNAVVHRFAQHYVKRALSLSPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRDTTAGLLSFVFFELARN

PAVLAKLREEIGTKFGLGKEARIEEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATRNTTLPRGGGPDGMSPILVTK

NQEVHYSVYSTHRMEEYYGKDANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASYVTTRLVQEFSSLWMDP

NTQYPPHKMSHLTMSLYDGCNVKME

 

>CYP52A35 Candida parapsilosis

65% to CYP52A21   Candida albicans

CPAG_05481

MIDSAIATYWYITIPSVFLLYIIISYINEQYLMRKFKAKPFTNYISGGFFGFQEGIAALKHKKAGTAIERYRDLYEELPN

PDVPTYKSFVFGTPLVFTKDPENIKAILATQFNDFSLGIRHAHFDPLLGDGIFTLDHQGWKDSRAMLRPQFARDQIAHVK

ALEPHFQFLLRHIEKNNGQFFDIQELFFRFTVDSATEFLFGSSVSSLQDESIGCDTTELDFVGRKEFPEAFNKSQLILST

RALLQKMYWIYNPKEFKKCNAIVHQFSDYYINKVLSCTPEEIEKQSGYVFLYELVKQTRNPKVLRDQALNILVAGRDTTA

GLLSFVLFELARNPDIYKKLRDEILDKFGTSNLEEITFESLKKCEYLKAVLNEALRMYPSVPRNYRVSTRNTTLPRGGGS

DGTSPIFIPKGRSVIYNIAATHMDPRYYGKDVEEFRPERWFEESTKKLGWAYLPFNGGPRICLGQQFALTEASYVVARLA

QTISKLELKDGYTYPPKKMTHLTMCMFDGVYVKMEKDATAAAAKSG

 

>CYP52A36 Candida parapsilosis

65% to CYP52A21   Candida albicans

CPAG_05482

MIETVSIANIVAYWYITIPAVLLGHFVIGYIHEQVLIRKLGAKPFANNVNGGFFGSALGVKSIVAKGKGRAVEFYQNLYH

VSPHTEVPTIKTYIFGTPIVFTKDPENIKAILATQFQDFCLGNRYHFLKPLLGKGIFTLDHEGWKDSRAILRPQFARDQI

AHVKALEPHFQFLLRHIEKNNGQFFDIQELFFRFTVDSATEFLFGSSVSSLQDESIGCDTSALDFSGRLEFAEAFNVSQV

YLSTRALLQRIYWLCNNQEFKKCNKIVHQFSDYYINKVLNCTPEEIEKQSGYVFLYELVKQTRDPIVLRDQALNILLTGR

DTTAGLLSFVLFELARNPEMYAKLRHEILDKFGTSNLEDITFENLKKCEYLKAILNETLRMYPSVPRNARVATRNTTLPR

GGGSDGTSPVFIPKGKAVIYNIAATHMDPRYYGKDVEEFRPERWFEESTKKLGWAYLPFNGGPRICLGQQFALTEASYVV

ARLAQTISKLELKEGYEYPPKKMTHLTMSMFDGVYVKMEL

 

>CYP52A37 Candida parapsilosis

58% to CYP52A12   Candida tropicalis

CPAG_05523

MALDLASISSFITIHLFTWYGAIISFALIYATVGKIKREIFIRKHGCKEAYVFPDGGPLGLVLAFILQKYKSDGTILDFA

LSLYEDYGKRTTFTAYLFGVRVVVSGEPENMKAVLATQFNDYSLGSRHAHFAPLLGDGIFTLDNEGWKESRQLLRPQFAR

EQISHVKMLEPHVQVLAEHIRKHKGQTFDIQELFFRLTVDASTEFLFGESVHSLYDESIGRKCDPEVAGFADAFNAAQVA

LGVRTYLQVMYFLYNPPSFWKNTRTVHNFARKFVDKALSLTPEQVEERTQKHGYTFLYELVKHTRDPKVLQDQLLNIMVA

GRDTTAGLLSFTIFELARHPKVWNRLKDEIYHHFGSGEDSDVENISFETLKQCTYLKWVLNEILRLYPSVPVNFRVANKD

TTLPVGGGPDGTSPVFIGKGTTFAYSPYMTHRLEKFYGKDSFEFVPERWENSPKLGWAYLPFNGGPRICLGQQFALTEAS

YVIVRLAQMFPNLSSSYNGPEPCRKLVHLTMSLMDGAEIQMS

 

>CYP52A38 Candida parapsilosis

74% to CYP52A25   Candida dublinensis

CPAG_02285

MSSILDQASDFGEKLLPYLTKWYSIVFIILLTYIPLLNIRNAYVGWKLGCQDPPKFRKAGFTGVFALIEAVKKKNQGRLV

DWGDEQFDEYPNHSMYLNVTGLLKIVLTVDPENIKAVLATQFNDFSLGTRHAHFAPLLGDGIFTLDGNGWKDSRAMLRPQ

FAREQIAHVKSLEPHLQILAKHIKSTNFQTFDLQELFFKFTVDTATEFLFGESVHSLYDDKLGIAPPNDIPGREHFADAF

NKSQKYLATRTYLQMFYFLINPKEFRDCNKKVQHLAQYFVNKALNFTPEELEEKSKDGYIFLYELVKQTKNPQVLQDQLL

NIMVAGRDTTAGLLSFTMYELARNPQVWQKLRQEIVENFGDGEDARVESITFETLKKCEYLKAVLNETLRLYPSVPVNFR

TATRDTTLPRGGGADGTKPIFVPKGSTVAYTVYKTHRLEEYYGKDSKEFKPERWENIKRLGWAYLPFNGGPRICLGQQFA

LTEASYVVARLVQMFPTLESQDDTYPPKKCVHLTMNLDEGVFVKMK

 

>CYP52C5 Candida parapsilosis

48% to CYP52C3  Candida albican

There is one 52C seq per Candida species except this species has two.  They all seem to be orthologs even though the percent identity is not high

CPAG_01250

MNYILYSALAAIYIIYRVIDHVQVTRKAQKYGCKPVAQVPKRTLLSWLGLQLILNLKRNHERGRAHEALDEAFDRLKTTT

FATYVGIEPNVFTVEPENFRHMVSSSHTSEFNLGVRLDVFSILLGDGIFSSEGTSWKHSRIMLRPFFARENIKQIQSMEP

FTQSIIKAIKQAPGMTIDMQEIFQDFTMDYTTHFLLGESCGCLKTYLGEPTSDSISTEVKSSFAETFDKGAEYMLQKLVM

GRYHFLYRPKQLYVDVKNQHAFVDYYVQKALRMNQQDLKDSSNDGTLFLYDLAQQTKDPKVLRDEILSIIVAGRSTTSAL

MTFLFLELGRHQDVFERLKGIVRAHFPDIESITFESIQQCEYLRWCLNETLRHDPPVPFNSRTVTQDTILPKGGGQDNES

PIFVRKGTKIVYPLWTAHRMEKYFGKDPYTFNPDRWSSLPANGGFAFMPFGAGPRNCLGQQLALLETSYVTIRLLQTFNS

LKADSGEVRKKSTATMVLMDGCKLTFG

 

>CYP52C6 Candida parapsilosis

50% to CYP52C2   Candida maltosa

CPAG_01665, 53% to to CYP52C5 this species

MTYIPYLIVIASYIIYKLLHRVQLHQRAIKLGCKPVKHIPPRNLLDRLGIYEIVHRKKLMHSGTTFEMLEEAFDRAQVKT

FEGTALFSSFIMTIEPENLRHMMSSAHFNEWNIGYRPQAFGILLGDGIFSSEGTQWKHSRVMLRPSFTKENIKQITKMEP

FAARVIDLIKQKSGQAIDMQDVFQFFTMDFTTDLLLGESSDSLKDALGQHSSTGIDPELKRQFSIAFDESSNYLMTRMIL

GPLMFLVNPKTLQDAIKIQHKFVDYYIEKALAMTSHELNEKSDDGTIFLYQLAHETKDPINLRNQILSIILAGRNTTSGL

MTFLFAQLSKNPEIYCKLRDIIRQDFPNVESITFDSIQNCDYLRWCINETLRINPSVPFESKTASVDTTLPRGGGVDEKA

PIFIAKGTRIVYPLWVANRNEEHFGENTGEFIPERWEQLPRSGGIAFMPFSVGPRACLGQSLALTEAAYITIRLLQTFDS

CESHLGPEPLRKTSSATMRLMDGCELSFK

 

>CYP56E4 Candida parapsilosis

65% to CYP56E1   Candida tropicalis

CPAG_05515

MINSLKGILWFRFVHNGTMVMFEFIKVVAASLLGFGLYLLLEIVFPPLCFPRSIPTIPFYVSFLSSWTNLDQRDIYNTYL

REKLEKYGAVKIYFASRWNILVTRPEYLIQVFKHEDIYAKSGNHLKIPGSVLASYTGDNVISAHGPLWKLYREVVSSSIQ

FPDLSTIHQNTNKFLDLLEQDLSTKSSILVNDLLQRYTLANVGDSILGVDFKALDGKESIMHQKIKYIKQQIFKPFFMNF

PYIENFPIPSRIKAKREVEGFRNWFGQNLSSNYNPERGNSAATKLAQALCNEKLTQKQFLDNAIIIMVAGHENPLLLMLS

LLFVVAKYQDVQELVRSETNPEQPFLCSVIYETLRLFPPLGQIINRCTTSSVCLGDIRIPRGVYVGYNNMATGRDRSVWG

ADADQFKPSRWGTTISEINTNYSSSKRLAKLPAFHGRKRACLGEKFALHESKLLLTSVLEKYKVSLDENWQEKITSAGPV

GPLGMKLKFEKV

 

>CYP61A1 Candida parapsilosis

79% to CYP61A2   C.albicans, N-term does not match

CPAG_03443

MDSFNQTVELAGSSHTNQIFNFLTSNKQINYFYQLYLSASWIQIVIASVV

LILSYDQVKYQLNKGSIAGPKFKIWPIIGP

FLESLDPKFEEYKAKWDSGALSCVSIFHKFVVIASTRDLARKILSSPKYVKPCVVDVAIKILRPTNWVFLDGKEHTDYRR

SLNGLFSQKALEVYIPVQEKYMDIYLERYLNYNGPRKFFPEFRELLCALSLRTFCGDYITEDQISLIADNYFRITAALEL

VNFPIIIPYTKTWYGKKIADDTMKIFENCAAKAKVHIGQGGKPTCVMDEWIYLMKEAKDKHLDDPESKLLIREFSNREIS

ETIFTFLFASQDASSSLACWLFQIVADRPDVVEKIREEQLRVRNGDMNVRLDLELIQKMKYTNNTVRETLRYRPPVLMVP

YVVKQNFPITESYTAPKGSMVIPTLYPALHDPEVFEDAESFIPERWENPTGDMDKRNWLPFGVGPHVCLGQKYVLMLFTG

MLGKFLVGADLVHHKTDLSEEIKVFATIFPKDDLILEFKRRV

 

>CYP501A4 Candida parapsilosis

50% to CYP501A2     Candida tropicalis

CPAG_01739

MPWTTVDGILLYIHSIARSPLTRWTCFVSVALLMIAIVYDYIYKPSKIKGIYPIPGELPIIGHLRLLRNNPSQVFLVWAT

RYDKSVFQIRLGNKRVIVVNDYDDVVELWMNHSCQNNSRPLSYTFHGLISSYQSFTVGSTPQSTTYKRKKRAIAQFLNSK

SISSYSLSICREIELTLFRLENVAINVDVDVCHYLKYFVLRCSVFLMYGLHLDCFGVDNALCNEIISNESEIIRMRSPIA

NVQDSVPLLKYLPNLTNASLAVQCKNKRERYMSELFARFEDKMNLGDCDSIGSFIGQLIAETGNKQLTDAEVYSICLTFI

SAGLDNTPLNLNYLLGILSQPSIGKAYQSKAINEILANCGGDVQKAWEQSNESTLKNVYVHALILETLRQFTVLPLSLPR

TTTKTIIYNGVEIPAGSQLFMNAYAANHDPKHFKCPFEFNPERWIDANGQISFTAKAYQHFSFGAGSRMCSGYSFAVKEM

YIFMVKFLLMFEVHPPDSKLMIADPFLSNENAHATSFEPRSHNIRLTLRRSRNQEWLHDTELVR

 

>CYP5217A3  Candida parapsilosis

56% to CYP5217A1   Candida dublinensis

CPAG_05265 yellow region seems too long (possible intron)

MLSELLQVFLSLLRLHLLWTLFSVLAIFSCYYFVYAPITSPFWNIPGPYIYRVSIIPALNHQRNGTWIQTVYKLHQIYGP

IVILSPKEISVYGPQYVNDIYIKNFPKSSFYANFTNHGHDNIFASLPNDQHLQYKKIIMRLYNKGNVMSPENNTRRILIE

STRKMLEYVYKSSIK

GESPDFASVAPTLNPHAKGHRKPWFNKSHRAKSL

GIEVFSLFGLFAMDVVSKFELGETNGSNLMD

NPSERDIILKHRQVSSMLFWTTLMPALWDFVATKTIMKCSEDIAKFRMSLYEIAEKHWANNGKNSTTLEVLKQNGLKGPR

AYSFLSDNLFAGHETTAVQLCYLVYELSRSGNHTMVEKLQHELYESFSKPNSHDDVIEDLEKLDKLEFLDALLLENSRVH

ASIPGAEPRVVDRVYSIAGVEIPKGTIISCLPYAMHRDSTVFPKHDYFIPDRWLKYPEESQEEFEQRKKAQHKFMMHFGK

GIRSCLGMHLAWMEMKLVVANLYWHFHSLLDDDWCEIKNDTEPIQLGKKNCGNNSTDQEMMCMNDAYTTTGPVNEECWIR

WVENS