Cryptococcus gattii P450s (5 sequences)

 

Synonym Cyptococcus neoformans serotype B (Broad Institute)

Note: This organism is causing serious illness in British Columbia Canada, Oregon and Washington state.  Previously it was found in Australia.

 

D. Nelson

April 23, 2010

 

>CYP51F1 Cryptococcus gattii

CNBG_0513 Transcript 1

96% to CYP51F1 Cryptococcus_neoformans var. neoformans B-3501A

revised to include 14 nucleotide micro exon

MSAIIPQVQQLLGQVAQYIPHWFTALPTSLKIVIAVIGIPAFIIGLNVFHQLCLPRRRDL

PPVVFHYIPWFGSAAYYGEDPYKFLFECRDKYGDLFTFILMGRRITVALGPKGNNLSLGG

KISQVSAEEAYTHLTTPVFGKGVVYDCPNEMLMQQKKFIKSGLTTESLQSYPPMITSECE

DFFTKEVGISSQKPSITLDLLKSMSELIILTASRTLQGKEVRESLNGQFAKYYEDLDGGF

TPLNFMFPNLPLPSYRRRDEAQKAMSDFYLKIMENRRKGESDHEHDMIENLQGCKYRNGV

PLSDRDVAHIMIALLMAGQHTSSATSSWTLLHLADRPDVVEALYQEQKEKLGNPDGTFRD

YKYEDLKELPIMDSIIRETLRM (0)

HAPIH (2)

SIYRKVLSDIPVPPSLAAPSENGQYIIPKGHYIMAAPGVSQMDPRIWQDAKVWNPAR

WHDEKGFAAAAMAQYTKAEQVDYGFGSVSKGTESPYQPFGAGRHRCVGEQFAYTQLSTIF

TYVVRNFTLKLAVPKFPETNYRTMIVQPNNPLVTFTLRNAEVKQEV*

 

>CYP61A1 Cryptococcus gattii

CNBG_1921 Transcript 1

98% to CYP61A1_Cryptococcus_neoformans var. neoformans B-3501A

MESHTILRPTAIPDLAAIKTWGLEGLTKAKFSFDSKTTTATILTLILSLLVLEQLVYRAK

KAHLPGAKWTIPVIGKFADSLNPTLANYKAQWNSGPLSAVSVFNIFIVIGSSNEMARKIL

NSPNHAEPCLVASAKKVLLPENWVFLHGKVHADYRKALNVLFTKQALSIYLPIQEKIYRS

YFNKWTSDPAPAQQYMMKMRDLNMETSLSVFIGPYLTEAQKQEINEKYWLITISLELVNF

PLAIPGTKVYNAIQARKIVMKYLSAASAASKIRMEDDDAEPECLLDHWVRAMILARRAQD

DGEETKLLSREYSDHEIAMVLLSFLFASQDAMSSALVYTFQLTADHPEVLAKVREEQYRV

RGNDLERPLTLDLLDDMVYTRATIKEVLRFRPPVIMVPYMTTKPFPVSPEYTAPKNSMII

PAFWNSLHDETCYPEPDRFLPERWLPQADGSAPIADSKPQNYLVWGSGPHKCIGGQYASM

HLAATLGTASVLMDWKHERTELSDEVQVIAAIFPKDHCLLKFTPRAPPS*

 

>CYP5139B1 Cryptococcus gattii

CNBG_1699 Transcript 1

91% to CYP5139B1 Cryptococcus neoformans var. neoformans B-3501A

MELFKTLHLETSQFFSNCIQPSPVACIALYTFTGVTILLFTIYLWLWPFQYATLHFRNLP

GPPSDSWFWGVVPTLIKSPPSVPHSMWTDEYGPTIRYRVALGAQRFLTIDPTALNYILSH

IDLFPKPSRVRKALSDLLGNGLLTAEGYTHKKQRKALNPSFSPAAIRGMVPVFYDKAYEL

KAKLLGIISDDETEQASPTPCIKEDEVEGGKKIDVMKYLGKTTLDVIGIVGFSYDFKALS

EPHNELSEAYSKMFQASMDANFWDFLRGAIPLVNKLPNKRATEIAARKAVTLRIGKKIVE

DKKREVMSAHSEGLEKREDIGNDLLSILIKANMASDVKPEQKLSDEEVLDQITTFMLAGN

ETSSTALTWILYSLTQHPECQERLREEVLAVADDRPSLETLNSLPYMDAVIRETLRLNAP

APGTLREAKQDTVIPLSMPVTGRDGKQINSVRINKGTVVFIPILTVNTSPAIWGPDARDF

NPDRHLKTASNSFGGANMHVPGVWGNMLSFLGGARNCIGYKLALAEISTILFVLMRSFEF

QELKSKPEVEKKASVVMRPRIKGEESAGLQMPLMVKPL*

 

>CYP5215A3 Cryptococcus gattii

CNBG_5445 Transcript 1

80% to CYP5215A1 Cryptococcus neoformans var. neoformans B-3501A

gene model revised. This is a pseudogene

MMPEQLSLHLPTTIES

MFALTVFALLWLLRVFVYKPFKSPLKEVSCPPGGTGSQGHIAEILDLQGTKVHDWIKAYG

TTFIVRGPFGVHHRIFSIDPRALNHVLKHTNIYTKSDLLRDLVRRYMEGGLIVGE

GEGEGHKVQRKVSQKLFSMGGPKSMGQVVQDKSNQ

RDILPNLCEDPTVSNP*PPVNPTLSTGS

REVDVYSTASRCIFDLVGSVGVDHQFGSLGNWEGSGGKLFQKYERMQLLCPGAMGF

RMLLSLTWPLIDKIWPSENIKRVNDAMGSLQKFAKEMMTERRLELSIIGSKKGDVPDRKG

LLTLMLRHNMAQKINAADSLQDHEISGQLSTFLFAGSETTAGTISFGLYDLTCHPDIQSR

LRTEILECGDNLPFDQIDELPYLDAVVKEIMRINPSLPGTVRQAQKDDIIPLAKPVTLTN

GKVVTDIHIRKGQLVHVPIEHLHTLEHIWGPAA*EFDPSRFFSSPQSSAFS &

QPTLGSHSAATSSARRDAVPSYVPEGPGIWPNFMTFIDGPRRCIGYKLAVMEIRTIIFTL

VGDFEIELMKGQHILRWNM (2)

MSNRPFVANTSWSKGSRLPLHFKLYKGGGQYEESVEFVSS*

 

>CYP5216A3 Cryptococcus gattii

CNBG_3491 Transcript 1

85% to CYP5216A1 Cryptococcus neoformans var. neoformans B-3501A

85% to CYP5216A2 Cryptococcus neoformans serotype A

MKGGRFLLPALFKTLILPPLIAALFTHHFPSLSLSLTALIYLISLPALYVFRSYVSLVTS

SRRAAALGAVDIPRVKGAWPLNIDVLLNWAKSGTEEEVGRMMVLMGRQYGGTYNTRVLGE

DQIISSDPKVIKHVLIDDFDNFVKGQKFKDRAQNFLGDGIFNSDGDGWKFHRSLMRPFFH

PMYISPLHFTVNIQAFFNSLPLYGKAFDMQACIGQLALELAIMWLCGEDMSADASNVERT

DEWRKAKREIGWAMTEAQKVVGKRVKIGTVWPLFEITHDPLERPMKVIRAFFRPIISQAL

NRKRQRCKSDDQEDAYMIDRLVEATDDVKLVEDQLINVLLASRDTLASLLIFSVYAIALH

PDIAARLKNEIFSVASRESEVTKDTIRQLRYSRAFINEVLRLFPPVPLNIRRTLRPSLLP

TTSHLVYMPANTSIILATILMQRDPAVWGEDALVFNPDRWLAGGLGKQRENFASWNLGPR

MCLGQPFALTITHTFLVYFFRHIAHVGTLVGQNTTIQLALDAQPPKSLLPKEWMTSEGSD

GRTRGGRDRVWIVADVVLAIKGGLWVRFGAENTE*