Jatropha curcas Cytochrome P450s

(crop plant used for making jet fuel in Brazil)

 

 

Jatropha curcas Genomic contigs of P450s (538 sequences)

From http://www.kazusa.or.jp/jatropha/

by P450 keyword search

 

some are revised with ESTs from NCBI.

~270 are in finished form (not able to extend or complete some of these)

 

480 Jatropha sequences are here after removing 20 false positives

(at the bottom of the file) and merging some sequences.

Ricinus communis sequences are added for help in assembly.

 

150 of these are complete or nearly complete sequences.

330 are partials and about 210 of these need to be looked at to see if

they can be further improved or finished.

 

Sequences that are most likely from the same gene have been joined even if no

overlap is present. This is the case when there is only one gene in the family

or subfamily.

 

The sequences are sorted in to 10 P450 clans

CYP51, CYP71, CYP72, CYP74, CYP85, CYP86, CYP97, CYP710, CYP711, CYP727

and inside each clan they are sorted into families.

 

The Clans CYP51, CYP74, CYP97, CYP710, CYP711 and CYP727 have only one Jatropha

family each

 

The CYP71 clan has 22 families: CYP71, CYP73, CYP75, CYP76, CYP77, CYP78, CYP79,

CYP80, CYP81, CYP82, CYP83, CYP84, CYP89, CYP92, CYP93, CYP98, CYP701, CYP703,

CYP706, CYP726, CYP736

 

The CYP72 clan has 8 families: CYP72, CYP709, CYP714, CYP715, CYP721, CYP734,

CYP735, CYP749

 

The CYP85 clan has 13 families: CYP85, CYP87, CYP88, CYP90, CYP707, CYP716, CYP718,

CYP720, CYP722, CYP724, CYP728, CYP729, CYP733

 

The CYP85 clan has 4 families: CYP86, CYP94, CYP96, CYP704

 

The sequences below include about 300 that have been checked for overlap with ESTs

and other contigs to assemble them as completely as possible.  Many are too short to

assemble into full sequences.  Some are clearly pseudogenes and others might be

pseudogenes, but the genome is incomplete, so it is not certain.

 

This is a work in progress.  There are still about 200 pieces that need scrutiny.

The sequences are not named yet.

 

The automated assembly output tagged many sequences as /pseudo.  This does not mean

they really are pseudogenes.  Each one will need to be examined.

 

David Nelson

Feb. 10, 2012

 

484 Jatropha sequences after subtracting 31 Ricinus communis sequences and 20 false positives

 

109 named orthologs

 

David Nelson

Revised June 10, 2012

 

CYP51 clan

 

CYP51 family (only one gene)

 

>CYP51G1

JcCD0121598.10 +

GW613182.1, GW619602.1, GW617740.1

finished

JcCB0430161.10 - 

86% to CYP51G1 Ricinus communis

MAAENNFLNMGLLIVATILVAKLISALIVPRSR

KRLPPVVKGWPLVGGLFRFLKGPIIMLREEYPKLGSVFTVNLAQRKITFLIGPEVSAHFF

KASETDLSQQEVYQFNVPTFGRGVVFDVDYSVRQEQFRFFTESLRVNKLKGYVDQMIMEA

EDYFSKWGDSGEVDLKYELEHLIILTASRCLLGREVRDKLFDDVSA

XXXXXXXXXXXXXXXXXXXXXX

AHRRRDRARKR

LAEIFANIIASRKLAGKSENDMLQSFIDSKYKDGRPTTDSEITGLLIAA

LFAGQHTSSITSTWTGAYLFRYKEYLSAVLEEQKTLMKKHGNKVDHDILSAMDVLYRCIK

EALRLHPPLIMLLRSSHSDFSVTARDGKEYDIPKGHIV

ATSPAFANRLSHVYKEPDRYDPDRFAVGREEDKVAGAFSYISFGGGRHGCLGEPF

AYLQIKAIWSHLLRNFEMELVSPFPEIDWNAMVVGVKGKVMVRYKRRELSVD*

 

CYP71 clan

 

CYP71 family (61 Jatropha sequences, 20 are complete or nearly complete)

10 Ricinus sequences

 

CYP71B

 

>CYP71B JcCA0015371.10 + short

BABX01006048.1

55% to CYP71B10 Arab. 
59% to CYP71AS1 Citrus sinensis
97% to CYP71AS   JcCB0119211.20, 96% to CYP71AS JcCA0295881.10
96% to JcCA0060021.10
complete, one stop
71% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASFHLHLWLSLLLLLLLPFIFLLKKKLNSKHLPPGPPRLPIIGNLHQLGTLPHYSLWQLSKK*GPV

MLLQFGQVPTLIISSAEAAKELIKINDLSSCSRPRLAGTGRLSYNYLDIAFTPYGDYWRE

IKKICVLELFSAKRVQSFQSVREEEVGLFIDSILKSSSSSSPVDLSEKIMSLTANVTCRV

AFGNSFAARGFTQERFQEVIHEAFAKLGGFSASDFFPYVGWIVDRVTGLHSKLERSFQEL

DEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAIIMNIFLGGV

DTGAIVLVWAMAELVRNPRVMRKAQEEIRRLIGDKTKVSERDINKLEYLKMVVKETLRLH

PPGTLLITRETMSQFSINGYEIYPKTRVHVNAWAIGRDPKTWKNPEEFFPERFIDNSIDF

RGQNYEFLPFGGGRRVCPGITMALSLVEIALANLLFCFDWKLPGNMKEADINMEEASGSG

LATHKKEALLLVPVKYELA

 

>CYP71B JHS05H19r.10 -

BABX01121443.1

Runs off the contig end

97% to JcCA0015371.10
finished
76% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

541 NIFLGGVDTGAIVLVWAMAELVRNPRVMRKAQEEIRRLIGDKTKVSERDINKLEYLKM VVKETLRLHPPGTLLITRETMSQFSINGYEIYPKTRVHVNAWAIGRDPKTWKNPEEFFPE RFIDNSIDFKGQHYEFLPFGGGRRGCPGMTMALSLVEIALANLLFCFDWKLPG 29 &

26 DMKEADINM 1

 

>CYP71B JcCB0003071.10 - 

63% to CYP71AS1 Citrus sinensis

complete

JcCA0228101.10 - 

76% to CYP71B76 gi|255570189|ref|XP_002526055.1  Ricinus communis

MFLYGLPFWVLVLLLPLLLVIIRKIKVQRENNQLPPSPAKLPIIGNLHQLRGSPHRAFWQ

LSKKYGPVMLLQFGSVPAVIISSAEAAEEALKIHDLSCCSRPPLAGAARLSYNYLDVAFS

PYGDYWREIRRICVLELFSTKRVQSFQFVREEEVGLLINSISQSSSSATPVDLTEKFLTL

TANITFRMAFGTSFGETDFHKDRFKKLIDDAQALLGSFSANEFFPYVGWIIDRISGYHAR

TENVFCQLDTFFQWIIDDHLKLGRTDKGQEDIIDVLLRIEREKTGVGSVQLTKDHIKAVL

MDLFLAGVNTGAVTLIWAMAELARSPRVMKKAQEEVRNVIRNKERVLESDVDELYYIKMV

IKETLRLHPPAPLLLPREAISNFKINGYRIHPKTLIQVNVWAIGRDPNYWKNPEEFFPER

FVDSSIDFKGQNFEFLPFGAGRRGCPALYMGTILVELVIANLLYCFDWRLPNGEENINME

EQAGPSLTVSKKEAVKLVPSRYLQ

 

>CYP71B JcCB0119211.20 + 

BABX01060935.1

59% to CYP71AS1   Citrus sinensis
FM890400 EST (3 aa diffs, two are adjacent and are errors that introduce a stop) 
complete
72% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLHIHMWLSLLLLLFPFIFLLKKKLNGKHLPPGPPRLPIIGNLHQLGTLPHYSLWQLS

KKYGPVMLLQFGQVPTLIISSAEAAKELIKINDLNSCSRPRLAGTGRLSYNYLDIAFTPY

GDYWREIKKICVLELFSAKRVQSFQSVREEEVGLFIDSILKSSSSSSPVDLSEKIMSLTA

NVTCRVAFGNSFATRGFTQERFQEVIHEALAKLGGFSASDFFPYVGWIVDRVTGLHSKLE

RSFQELDEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAIIMN

IFLGGVDTGAIVLVWAMAELVRNPRVMRKAQEEIRRLIGDKTKVSERDINKLEYLKMVVK

ETLRLHPPGTLLITRETMSQFSINGYEIYPKTRVHVNVWAIGRDPKTWKNPEEFFPERFI

DNSIDFRGQNYEFLPFGGGRRVCPGITMALSLVEIALANLLFCFDWKLPGNMKEADINME

EASGSGLAAHKKEALLLVPVKYELA

 

>CYP71B JcCB0480541.10 –  exon 1

BABX01085181.1

100% to CYP71AS JcCB0119211.20
finished
71% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

LSHLQKQQKELIKINDLNSCSRPRLAGTGRLSYNYLDIAFTPYGDYWREIKKICVLELFS

AKRVQSFQSVREEEVGLFIDSILKSSSSSSPVDLSEKIMSLTANVTCRVAFGNSFATRGF

TQERFQEVIHEALAKLGGFSASDFFPYVGWIVDRVTGLHSKLERSFQELDEFYQKIIEDH

IQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAII

 

>CYP71B JcCB0793041.10 +  exon 1

BABX01105908.1

97% to CYP71AS JcCB0119211.20
finished
69% to CYP71B64 gi|255564478|ref|XP_002523235.1 Ricinus communis

MASFHLHMWLSLLLLLLLPFIFLLKKKLNSKHLPPGPPRLPIIGNLHQLGTLPHYSLWQL

SKKYGPVMLLQFGQVPTLIISSAEAAKELIKINDLNSCSRPRLAGTGRLSYNYLDIAFTP

YGDYWREIKKICVLELFSAKRVQSFQSVREEEVDLFIDSILKCSSSSSPVDLSEKIMSLT

ANVTCRVAFGNSFAARGFTQERFQEVIHEALAKLGGFSASDFFLYVGWIVDRVTGLHSKL

ERSFQELDEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAIIM

(0)

 

>CYP71B JcCA0079531.20 + exon 1

BABX01029891 

92% to CYP71AS JcCB0119211.20
FINISHED
70% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLPQHVWLALILLLFPLLFLLKKKLTSNNFPPGPPGLPIIGNLHQLGALPHYSLWQLS

KKYGPVMLLQYGRVPTVIISSAEAAKELFRINDLNSCSRPRMAGTGRLSYNYLDIAFTPY

GDYWREIKKICVLELFSAKRVQSFQSVREEEVGLFIGSILKASSSSSPVDLSEKIMSLTA

NVTCRVAFGNSFATRGFTQERFQEVIHEALAKLGGFSASDFFPYVGWIVDRVTGLHSKLE

RSFQELDEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAII

 

>CYP71B JcCB0682821.10 + exon 1, partial

BABX01098061 runs off the contig end at KGK

83% to JcCA0204311.10

100% to JcCA0079531.20
finished
73% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis
MASLPQHVWLALILLLFPLLFLLKKKLTSNNFPPGPPGLPIIGNLHQLGALPHYSLWQLS 

KKYGPVMLLQYGRVPTVIISSAEAAKELFRINDLNSCSRPRMAGTGRLSYNYLDIAFTPY

GDYWREIKKICVLELFSAKRVQSFQSVREEEVGLFIGSILKASSSSSPVDLSEKIMSLTA

NVTCRVAFGNSFATRGFTQERFQEVIHEALAKLGGFSASDFFPYVGWIVDRVTGLHSKLE

RSFQELDEFYQKIIEDHIQKGK

 

>CYP71B JcCB0201851.20 +

BABX01066242 one frameshift at &

58% to CYP71AS1   Citrus sinensis
71% to XM_002523189 Ricinus communis, 73% to JcCB0119211.20
finished, complete
72% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MVQQVWLPPLLLLLFPLLFLLKKKLNSKNLPPGPPGLPIIGNLHQIGTLPHYSLWQLSKKYGHV

MLLQFGRVPTVIISSAEAA & 2234

2236 KELFKTNDLYSCSRPLLAGTGRLSYNFLDIVFTPYGD

YWREVRKICVIELFSAKRVQSFQSIREEEVGLLIDSILKASSSSSPVNLSEMTLALTANI

TCREAFGKSFEARGFSQERFQEVIHEGFAMLGSFSAADFFPYVGWIVDRLTGLHARLEKN

FQEFDEFYQKIIDDHIQKERNEKEHGQEDIIDVLLDLERDQNDHQSSAFRLSQDRIKAIL

MNIFLAGVDTGAITLVWAMAELIKNPRVMKKAQKEIRTLIGNKREVSESDIDKLYYLKMV

LKETLRLHPPATLLVPRESISKFNIGGYEVYPKTRIQVNVWAIGIDPEVWKDAERFFPER

FMDKDIDYKGQHYEFLPFGAGRRGCPGISMGMVMVERALANLLYWFDWRLPNDMKEEDFS

MEEEAGLATYKKVSLVLFPVKYQP

 

>CYP71B64 XM_002523189 Ricinus communis, XP_002523235.1
74% to JcCA0021071.10, 73% to JcCB0443791.10, 72% to JcCB0119211.20

MASLWQLSLLLLFPLLFFFLKRKLQAKTRIKNHPPGPPSLPIIG                      NFHQLGVLPHQSLWQHSKKYGPVMLVKLGRVPTVIISSSGAAKELFKTHDLNSCSRPL                      LTGSGKLSYNYLDIAFTPYGDYWRDMRKLCVLELFSAKRVQSFQFIREQEVSLLIDSI                      SKSSSSCPVDLSEKAMTLTANIICRAAFGKSFQERGLNHERFQEAIHEGLAMLGSFSA                      ADFFPYVGWIVDRITGLHARLERNFQEFDTFYQKIIDDHIQKGRKDGSQQVEDIIDVL                      LELEKSHREEFGAFQFSKDHIKAILMNIFLAGVDTGAITLVWAMTELIRNPRVMKKAQ                      EEIRSCIGDKRKVSEIDIEKFGYLKIVLKETLRIHPPSVLLIPRETMAQFSINGYDIY                      PKTRIQVNVWAMGRDPKIWKNPQEFYPERFLDSSIDYKGMNYELLPFGGGRRGCPGIT                      MGMTTVELALANLLFYFDWKLPYNMKIEDINMEEAPGLTIHKKEPLLLVPTIYQPVSKN

 

>CYP71B65 EE255099 Ricinus communis EST, XP_002523238.1
96% to XM_002523189 Ricinus communis

MASLWQLSLLLLLPLLFFFLKRKLQAKTRIKNHPPGPPSLPIIGNFHQLGVLPHQSLCQYSKKYGSVMLV

KLGRVPTVIISSSGAAKELFKTHDLNSCSRPLLTGSGKLSYNYLDIAFTPYGDYWRDMRKLCVLELFSAK

RVQSFQFIREQEVSLLIDSISKSSSSCPVDLSEKAMTLTANIICRAAFGKSFQERGLGHERFQEVIHEGL

ATLGSFCAADFFPYVGWIVDRITGLHARLERNFQEFDTFYQKIIDDHIQKGRKDGSQQVEDIIDVLLELE

KSHREEFGAFQFSKDHIKAILMNIFLAGVDTGAITLVWAMTELIRNPRVMKKAQEEIRSCIGDKRKVSEI

DIEKLGYLKIVLKETLRIHPPGVLLIPRETMAQFSINGYDIYPKTRIQVNVWAMGRDPKIWKNPQEFYPE

RFLDSSIDYKGMNYELLPFGGGRRGCPGITMGMTTVELALANLLFYFDWKLPYNMKIEDINMEEAPGLTI

HKKEPLLLVPTIYQPVSKN

 

>CYP71B JcCA0021071.10 – one frameshift at &

BABX01012429.1

78% to CYP71AS JcCB0119211.20, 74% to XM_002523189 Ricinus communis
58% to CYP71AS1 Citrus sinensis
complete
74% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLPLHLCLPLLLLLLLLLFPLLLLLKKKLYSTHLPPGPLRLPIIGNLHQLGTLPHYSF

WQLSKKYGPVMLLQFGRVPTVIISSAEAAKELIKTNDLSSCSRPRLAGTGRLSYNFLDIA

FTPYGDYWREMRKICVLELFSAKRVQSFQSIREEEVGLLIDSILKSSSSSFPVDLSEKTM

SLTANVICRVAFGKSFQERGFKHERFQEVVREGFAMLGSFTAADFFPHVGWVVDRLTGLH

ARSDRVFKEFDEFYQKIIDDHIQKGKEDPGHEDIIDVLLDLERYQTESGGI &

FSQNHIKAILM (0)

NIFLAGVDTGAIVLIWAMAELIRDSRVMRKAQLEIRSLIGDKRNVSES

DTGKLDYLKLVLKETMRLHPPVPLLLPREAISQFSINGYEVCPKTQIRVNVWAIGRDPKI

WKNPEEFIPERFINSSIDFRGQNYEFLPFGGGRRICPGMTMGMTLVELALANLLFCFDWK

LPYNMKEEDINMEEEFGLTNRKKEALLLVPIKYHLE

 

>CYP71B JcCA0060021.10 –

BABX01027654.1

58% to CYP71AS1   Citrus sinensis 
complete
70% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASFHLHMWLSLLLLLLLPFIFLLKKKLTSKHLPPCPPRLPIIGNLHQLGTLPHYSLWQL

SKKYGPVMLLQFGQVPALIISSAEAAKELIKINDLSSCSRPRLAGTGRLSYNYLDIAFTP

YGDYWREIKKICVIELFSAKRVQSFQSVREEEVGLFIDSILKSSSSSSPVDLSEKIMSLI

ANVTCRVAFGNSFATRGFTQERFQEVIHEALAKLGGFFASDFFPYVGWIVDRITGLHSKL

ERSFQELDEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGIQFSKSHIKAIIM

NIFLGGVDTGAIVLVWAMAELVRNPRVMRKAHEEIRRLIGDKTKVSERDINKLEYLKMVV

KETLRLHPPGPLLITRETMSQFSINGYEIYPKTRVHVNAWAIGRDPKTWKNPEEFFPERF

IDNSIDFRGQNYEFLPFGGGRRVCPGMTMALCLLEIALANLLFCFDWKLPGNMKEADINM

EEASGSGLATHKKEALLLVPVKYELA

 

>CYP71B CYP71 JcCB0443791.10 +

BABX01082262.1 almost same as BABX01026019

BAD EXON BOUNDARY AT NIF

COMPLETE

58% TO CYP71AS1 Citrus sinensis
73% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MVSPSLLLWLLPLLLALFLVLEGGLGTKRKGKNLPPGPPRLPIIGNLHQLGALPHYSL

WQLSKKYGPVMLLQFGRVPTVIISSAEAAKELFKANDLNSCSRPRLAGAGRLSYSYLDIA

FTPYGDYWREMRKICVLQLFSAKRVQSFQSFREEEVGFLIDSILKSSSASSSPVDLSEKT

LSLTANVTCRVAFGETFQERGLAHERFQEVIHEGLAMLGSFSAADFFPYVGWIVDRLTGL

HERLERNFQEFDELYQEIIDDHIQKGKSDPGHEDIVDVLLDMEKDQTESGGIQFSQNHIK

AILM (0)

NIFIAGADTGAIVLVWA

MAELVRNPRVMRKAQDEIRTLLGNKTKVSETDTDKLEYLKMVLKETMRLHPPGTLL

IPRETMSQFSINGYEIHPKTRIQVNVWAIGRDPKPWKNPEEFFPERFIDNSIDFRGQNYE

LLPFGGGRRGCPGVTMGLALVELAFANLLFCFDWKLPNNMEAEINMEEILVLLLIRKKL

 

>CYP71B JcCA0094041.10 + one frameshift

BABX01031526

90% to CYP71 JcCB0443791.10
complete
75% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLHLHMWLSLLLLLFHFIFLLKKKLNSKHLPPSPPRLPIIGNLHQLGALPHYSLWQLS

KKYGPVMLLQFGRVPTVIISSAEAAKELFKANDLNSCSRPRLAGTGRLSYSYLDIALIPY

GDYWREMRNICVLQLFSAKRVQSFQSIREEEVRFLIDSILKSSSASSSPVHLSEKTLSLT

ANVTCRVAFGESFQERGLAHERFQEVIHEGLAMLGSFSAADFFPYVGWIVDRLTGLHERL

ERNFQEFDEFYQKIIDDHIQKGKADPGHEDIIDVSLEMEKDQTESGGIQFSQYHIKAILM

NIFIAGVDTGAIVLVWAMAELVRNPRAMRKAQEEIRTLLGNKTKVSETDIDKLEYLKMVL

KETMRLHPPGTLLIPRETI &

SQFSINGYEIHPKTRIQVNVWAIGRDPKTW

KNPEEFFPERLIDNSIDFRGQNYELLPFGGGRRGCPGITMGLALVELALANLLFCFDWKL

PNNMEAEINMEEIFWSYYS*

 

>CYP71B JcCB0095131.10 + 

BABX01115200

94% to JcCB0000691.60, 83% to JcCA0021071.10

finished

72% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

1794 DIFLAGVDTGAVV

MVWAMTELVRNPRVMRKAQEEIRSLIGEKIKVSESDTDKFIYLKSVLKETMRLHPPVPLL

LPREAISQFNINDYEIHPKTRIQVNVWAIGKDPKIWKNPEEFIPERFIDNSIDFRGQNYE

FLPFGGGRRSCPGITMGLALVELALANLLFCFDWELPYNMKEADINMEEKFGLTNHKKEA

LMLVPIKYHHLLYNKE* 2423

 

>CYP71B JcCB0095131.20 -  pseudogene

BABX01115200

46% to CYP83F1v1   Populus trichocarpa
93% to JcCB0443791.10, 80% to JcCA0021071.10
finished
75% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

3351 SSASSSPVDLSEKTLSLTANVTCRVAFGETFQERGLAHERFQEVIHEGLAMLGSFSAADF

FPYVGW &

VDRLIGLHERLERNLQEFDEFYQKIIDDHIQKGKADPGHEDIIDVLLEMEK

DQTESGRIQFSQNHIKAILM (0) 2937

 

>CYP71B JcCA0204311.10 + exon 1

BABX01011701

83% to JcCB0682821.10 
79% to JcCA0079531.20
77% to JcCA0094041.10   + 
76% to CYP71 JcCB0443791.10 
no ESTs to extend the seq., finished

JcCD0092500.10 +

72% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

METWRPTIASLPQHVWLVLLLLLFPLLFLLKKKLTSNNLP

PGPPGLPIIGNLHQLGALPHYSLWQLSKKYGPIMLLQFGRVPTLVISSAEAAKELLKKND

LNSFSRPRLTGTGRLSYNYLDMAFAPYGDYWREVRKICVLELFSAKRVQSFQSVREEEVG

IFIDSILKASSSSSPVDLSEMSLTLTANITCRAAFGKSLKARGFTQERFQEVIHEGFAML

GSFSAADFFPYVGWIVDRLTGLHARLEKNFQEFDEFYQKIIDDHIQKERNETEDIIDVLL

DLERDQSSAFQLSQDHIKAILM (0)

 

>CYP71B JcCA0295881.10 + one frameshift

BABX01021939.1

96% to CYP71AS JcCB0119211.20
complete
71% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASFHLHMWLSLLLLLLLPFIFLLKKKLNSKHLPPGPPRLPIIGNLHQLGTLPHYSLWQL

SKKYGPVMLLQFGQVPTLIISSAEAAKELIKINDLNSCSRPRLAGTGRLSYNYLDIAFTP

YGDYWREIK &

KICVLELFSAKRVQSFQSVREEEVGLFIDSILKSSSSSS

PVDLSEKIMSLTANVTCRVAFGNSFATRGFTQERFQEVIHEALAKLGGFSASDFFPYVGW

IVDRVTGLHSKLERSFQELDEFYQKIIEDHIQKGKEKHGHQDIVDVLLDLERYQTESEGI

QFSKSHIKAIIM (0)

NIFLGGVDTGAIVLVWAMAELVRNP

RVMRKAQEEIRRLIGDKTKVSERDINKLEYLKMVVKETLRLHPPGPLLITRETMSQFSIN

GYEIYPKTRVHVNAWAIGRDPKTWKNPEEFFPERFIDNSIDFRGQNYEFLPFGSGRRSCP

GMTMALSLVEIALANLLFCFDWKLPGNMKEADITTEEASGPGLTTHKKEALLLVPIKYQP

AXKKKKE*

 

>CYP71B JcCA0045771.10 - pseudogene

BABX01026019

57% to CYP71AS1   Citrus sinensis
98% to JcCB0443791.10
finished
73% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

LVSPSLLLWLLPLLLALFLV*EGGLGTKRKGKNLPPGPPRLPIIGNLHQLGALPHYSLWQLSKK

YGPVMLLQFGRVPTVIISSAEAAKELFKANDLNSCSRPRLAGIGRLSYSYLDIAFTPYGD

*WREMRKICVLQLFSAKRVQSFQSTREEEVGFLIDSILKSSSASSSPVDLSEKTFSLTAN

VTCRVAFGESFQERGLAHERFQEVIHEGLAMLGSFSAADFFPYVGWIVDRLTGLHERLER

NFQEFDELYQEIIDDHIQKGKSDPGHEDIVDVLLDMEKDQTESGGIQFSQNHIKAILM (?)

NIFIAGADTGAIVLVWA

MAELVRNPRVMRKAQDEIRTLLGNKTKVSETDTDKLEYLKMVLKETMRLHPPGTLLIPRETM

SQFSINGYEIHPKTRIQVNVWAIGRDPKPWKNPEEFFPERFIDNSIDFRGQNYELLPFGG

GRRGCPGVTMGLALVELAFANLLFCFDWKLPNNMEAEINMEEILVLLLIRKKL*

 

>CYP71B JcCA0126681.10 + 

BABX01029891

56% to CYP71AS1 Citrus sinensis, 54% to CYP71B10 Arab. 
complete
69% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLPQHVWLALILLLFPLLFLLKKKLTSNNFPPGPPGLPIIGNLHQLGALPHYSLWQLSKKYGPV

MLLQYGRVPTVIISSAEAAKELFRINDLNSCSRPRMAGTGRLSYNYLDIAFTPYGDYWRE

IKKICVLELFSAKRVQSFQSVREEEVGLFIDSILKASSSSSPVDLTEKTISLTANVTCRV

ALGNSFEASRFTQKVIHEALAKLECFSASDFFPYVGWIVDRVTGLHAELERNFQKLDEFY

QKIIDDHIQREKRSMDIKILSMFLLDLERYQPEPGGIQFTKNHIKAIIMNIFLGGLDTGA

IVLVWAVAELVRNPQVMRKAQEEIRRLIGNKTKVSESDIHKLEYLKMVVKETLRLHPPGT

LLIPRENMSQFSINGYEIHPKTRVEVNVWAIGRDPKAWKNPEEFFPERFIDKSIDFIGQN

YEFLPFGSGRRSCPCMTMALSLVEIALANLLFCFDWKLPGNMKEADINMEEASGSGLTTH

KKEALLLVPIKYELA

 

>CYP71B69 XM_002523196 Ricinus communis, XP_002523242.1

MASLWLLSLLLLFPLCLFFLKKKLQVKTHTKNHPPGPPCLPIIG                      NLHQLGVLPHQPLWQYSKKYGPVMLVKLGRVPTVIISSSEAAKELLKTHDLSSCSRPL                      LTGAGKLSYNYLDIAFTPYGDYWRDMRKLCVLELFSIKRVQSFKFAREEEVSLLIDSV                      LKSSSSPVDLSEKAMTLTANIICRVAFGKSFQERGFGHERFQEVIREAVALLGSFYAA                      DYFPYVGWIVDRITGLHARLERSFQEFDTFYQKVIDDHIQKGTRDETQQEEDIIDVLL                      ELEKSHRERSGGFQFSKDNIKAILMNIFLAGVHSTATTLVWAMSELIRNPRVIEKAQT                      EIRNCIGDKRKVCESKIEKFEYLKLILKETLRLHPPGPLVVPRETMTQFSINGYDVHP                      KTRIQVNVWAIGRDPTKWRNPEEFYPERFIDSSVDYRGMHYELLPFGGGRRGCPGISM                      GIAIVELALANLLFCFNWRLPYLWKNGNIFTIFVNYKADINRNDAFYVI

 

>CYP71B JcCB0199391.10 - 

70% to XM_002522168 Ricinus communis

JHL01P16f.10 + 

BABX01066084.1 runs off the end, BABX01120767.1

No ESTs to extend seq, finished

72% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

MASLSLHMWLPLLLLLFPFIFLLKKKLNSKHLPPGPPGLPIIGNLHQLGTLPPYSLWQLS

KKYGPVMLLQLGQIPTVIISSAEAAKELIKTNDLNSCSRPCSAATGRLSYNFLDISFAPY

GDYWREMRKICVLELFSAKRVQSFQSIREEEVGFLIDSILKSSLKSSSPVDLSEKTLSLT

ANVICRAAFGKSFQERGFNHDRFQEAIHEAFAMLGSFPATDFFPHVGWIVDRLTGLHTTL

ERVFQEFDEFYQKIIDDHIQMGKADPGHEDIIDVLLDLERHRTASGGIQISRNHIKAILM (0)

 

>CYP71B78 XM_002522168 Ricinus communis, XP_002522214.1  

MASLWLLSLLLLFPLFFFSLRKKLQAKTHTKNHLPGPPSLPIIG                      NFHQLGVLPHQSLWQYSKRYGPVMLVKLGRVPTVIISSSEAAKELLKTHDLSSCSRPL                      LTSTGKLSYNYLDIAFTPYGDYWRNMRKLCVLELFSTKRVESFQFVREEEVSLLIDSI                      SKSSCSPVDLSEKAIFLTANITCRVAFGKSFQERGFGHERFQEVIREAFALMGSFYAA                      DFFPYVGWFVDRITGLHARLERNFQEFDTFYQKIIDDHIQKGTRDKPEQEEDITDILL                      ELERSHREESGAFQFSKNHIKAILM

 

>CYP71B JcCB0717711.10 - 

BABX01100561.1 runs off the end, no ESTs to extend

finished

76% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

SCSRPRLAGTGRLSYTYLDIAFTSYGDYWREMRKICVLELFSAKRVQSFQSIRTEEVGFL

IDSILKSSSASSSPVDLSEKTLSLTANVTCRVAFGESFQERGFAHERFQEVIHEGLAMLG

SFSAADFFPYVGWIVDRLTGLHERLERNFQEFMSSTKRLLMIISKREKQILDLKISLMSC

WKWRKIKLNLVEFNSLKIISKQFSW ()

KKTLKPWKNPEEFFPERFIDNSIDFRGQNYELLPF

GGGRRGCPGITMGLALVELALANLLFCFDWKLPNNMEAEINMEEILVLLLIRKKL*

 

>CYP71B JcCA0204781.10 +

BABX01011754.1 ,  no  ESTs

74% to XM_002523192 Ricinus communis

finished

74% to CYP71B65   gi|255564484|ref|XP_002523238.1  Ricinus communis

TKNHIKAIIM (0)

NIFLGGVDTGAIVLVWAMAELVRNPRVMRKAQEEIRRLIGDKTKVSERDI

NKLEYLKMVVKETLRLHPPGPLLITRETMSQFSINGYEIYPKTRVHVNAWAIGRDPKTWK

NPEEFFPERFIDNSIDFRGQNYEFLPFGGGRRVCPGMTMALSLLEIALANLLFCFDWKLP

GNMKEADINMEEASGSGLATHKKEALLLVPVKYELA*

 

>CYP71B65 XM_002523192 Ricinus communis cytochrome P450, putative, mRNA

XP_002523238.1

97% to XM_002523189 Ricinus communis
99% to EE255099 Ricinus communis EST 1 aa diff at end
69-71% to many Jatropha sequences

MASLWQLSLLLLLPLLFFFLKRKLQAKTRIKNHPPGPPSLPIIG                      NFHQLGVLPHQSLCQYSKKYGSVMLVKLGRVPTVIISSSGAAKELFKTHDLNSCSRPL                      LTGSGKLSYNYLDIAFTPYGDYWRDMRKLCVLELFSAKRVQSFQFIREQEVSLLIDSI                      SKSSSSCPVDLSEKAMTLTANIICRAAFGKSFQERGLGHERFQEVIHEGLATLGSFCA                      ADFFPYVGWIVDRITGLHARLERNFQEFDTFYQKIIDDHIQKGRKDGSQQVEDIIDVL                      LELEKSHREEFGAFQFSKDHIKAILMNIFLAGVDTGAITLVWAMTELIRNPRVMKKAQ                      EEIRSCIGDKRKVSEIDIEKLGYLKIVLKETLRIHPPGVLLIPRETMAQFSINGYDIY                      PKTRIQVNVWAMGRDPKIWKNPQEFYPERFLDSSIDYKGMNYELLPFGGGRRGCPGIT                      MGMTTVELALANLLFYFDWKLPYNMKIEDINMEEAPGLTIHKKEPLLLVPTIYQPVSK                     

N

 

>CYP71B JcCA0106771.10 -  

64% to CYP71AS1 Citrus sinensis

BABX01000770.1

84% to CYP71AS JcCB0119211.20 
91% to CYP71   JcCA0094041.10

74% to Ricinus communis XM_002523192

finished

73% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

2180 (1) VMRKAQEEIRRLIGNKTKVSESDIHKLEYLK

MVVKETLRLHPPGTLLIPRETMSQFSINGYEIHPKTRIQVNVWAIERDPKTWKNPEEFFPERFIDNSIDFR (0)

1875 SQNYESLPFGGGRRGCPGIIMGLALVELALANLLFCIDWKLPNNMEAQINMEEIFD 1708

1707 LTAHKKEALMLVPIKYQLI* 1648

 

>CYP71B JcCB0000691.50 - pseudogene

BABX01098658.1

Pseudogene

48% to CYP71B34 Arab.
53% to CYP71AS5 Vitis
71% to JcCA0094041.10
finished
64% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

17930 NEFYQKIIDDHIEKGKEDPGHDDI &

17859 IIDVLLDLERDQADSG 17812 &

      GLPFSRHHIKALLM (0) 17769

16491 (0) NLFIAGVDSGAIILVWGMAELVRNPGVMRKAQEEIRTVVGDKRKISESDIHKLEYLE 16321

16320 IMLKETLKLHPLGPLLIPREAMKQFSIDGYEIRPRT*IQVN 16198

      (50 aa deletion)

16180 MGLALVELTLANLLFYFHWKLPNNMKEANIVDMEE 16076

 

>CYP71B JcCB0000691.60 -

BABX01098658.1

second gene runs off the end of the contig

58% to CYP71B34 Arab.
73% to XM_002523189.1 Ricinus communis
83% to JcCA0021071.10
no ESTs extend seq
finished
73% TO CYP71B64   gi|255564478|ref|XP_002523235.1  Ricinus communis

22902 MLGSFPATDFFPHVGWIVDRLTGLHARLERVFQEFDEFYQKIIDDHVQKGKEDPGHEDII 22723

22722 DILLDLERHRTASGGIQISRNHIKAILM (0) 22639

DIFLAGVDTGAIVMVWAITELVRNPRVM

RKAQEEIRSLIGEKIKVSESDTDKFVYLKSVLKETMRLHPPVPLLLPREAISQFNINDCE

IHPKTRIQVNVWAIGRDPKIWKNPEEFIPERFIDNSIDFRGQNYEFLPFGGGRRSCPGTT

MALALVELALANLLFCFDWKLPYNMKEADINMEEKFGLTNHKKEALMLVPIKYQLVYNKE* 19564

 

>CYP71B JcCA0074451.10 +   

BABX01029308.1

96% to JcCB0201851.20
70% to XM_002523189 Ricinus communis
no ESTs
finished
70% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

3    QSSAFRLSQDHIKAILM (0)

1504 NIFLAGVDTGVITLVWAMAELIKNPRVMKKAQKEIRTLIGNKREVSE

SDIDKLYYLKMVLKETLRLHPPATLLIPRESISKFNIDGYEVYPKTRIQVNVWAIGRDPE

VWKDAERFFPERFMDKDIDYKGQHYEFLPFGAGRRGCPGISMGMAMVELALANLLYWFDW

RLPNDMKEEDFSMEEEAGLATYKKVSLVLFPVKYQP* 2215

 

>CYP71B JcCD0057194.10 + pseudogene

BABX01140601.1

97% to JcCA0074451.10
71% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

739 NIFLAGVDTGXITLVWAMAELIKNPRVMKKAQKEIRTLIGNKREVSESDIDKLY YLKMVLKETLRLHPPATLLIPRESISKFNIDGYEVYPKTRIQVNVWAIGRDPEVWKDAER FFPERFMDKDIDYKGQHYEFLPFGAGRRG & 1167

1176 GISI & 1187

1190 GMAMVELALANLLYWF 1237 &

1239 DWRLPNDMKE 1268

 

>CYP71B JcCB0220681.10 + possible pseudogene or seq errors at N-term part

BABX01067397.1

93% to JcCA0021071.10
72% to XM_002523189 Ricinus communis
no ESTs
finished
72% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

1 FYQKIIDDHIQKGKE 45 &

51 PGHEDIIDVLLD & 86

101 KTESGGIPFFPKNHI 145 &

147 KAMLM (0)

NIFLAGVDTGAIVLIWAMAELIRDSRVMRKAQLEIRSLIGDKRNVSESDTG KLDYLKLVLKETMRLHPPVPLLVPREAISQFSINGYEVYPKTQIRVNVWAIGRDPKIWKN PEEFSPERFINSSIDFRGQNYEFLPFGGGRRICPGMTMGMTLVELALANLLFCFDWKLPY NMKEEDINMEEEFGLTAEKKEALLLVPIKYHLE*

 

>CYP71B JcCD0055407.10 +

BABX01140383.1

No ESTs

66% to XM_002523192 Ricinus communis

finished
65% to CYP71B65 gi|255564484|ref|XP_002523238.1  Ricinus communis

175 (0) DIFLAGVDTAAIILVWAMAELIKNPRIMKKAQEEIRTLIGNKREFSKSNIDKLDYLKXIL

KETLRLHSSGALLIPRESISKFNIGGYEVYPKTRIEVNAWAIGRDPKVWKDAEKFFPERF

VDKDIDYKGQHYESLPFGAGRRSCPGMSMGMAMAELALANLLYWFDWRLPNDMKEEDLSM

EEKASLATYKKVPLVLVPVKYQP* 785

 

>CYP71B JcCB0278621.10 - pseudogene

BABX01071217.1

94% to JcCA0126681.10
67% to XM_002523189 Ricinus communis
finished
67% TO CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis

VWLALILLLFPLLFLLKKKLTSNNFPPGPPGLPII GNLHQLGALPHYSLWQLSKKYGPVMLLQYGRVPTVIISSAEAAKELFRINDLNSCSRPRM AGTGRLSYNYLDIAFTPYGDYWREIKKICVLELFSAKRVQSFQSVREEEVGLFIDSILKA SSSSSSPVDLTEKTISLTANVTCRVAFGNSIEASGFTQKVIHEALAKL*CFSASDFFPYV GWIVDRVTGLHAELERNFQKLDEFYQKIIDDHIQK 450 &

448 GKEKHGHQDIVDFLLDLERYQPEPGGIQFTKNHIKAIIM (0)

 

>CYP71B JcCB0471061.10 - pseudogene N-TERM

BABX01084445.1

42% to CYP71B19 Arab.

52% to JcCB0003071.10
finished
54% TO CYP71B76 gi|255570189|ref|XP_002526055.1  Ricinus communis

856 IIIPSVLLLLKKKA**NKKLLPGPPKLQIIGHLHQIGILPYSL 728 &

725 SQLSKEYGPIMLLHLVPLPTPVISSAEAAKEVLKTYDLDCCSRPRLVGAKRLTHDYLE 552

551 IAFSPYSELERD 516

504 LLSLKRVQSFRFIREEEVNLLMSLVSQLSSSATPFNLTEKSHALAMAFGTTFGG 343

342 SDFGNDRLQELIREAEAVA*CLSAEDYFGY 253

 

>CYP71B JcCB0282671.10 - pseudogene

BABX01071516.1

39% to CYP71AS JcCB0003071.10 mid region
36% to CYP71AS7v1 Vitis
46% to JcCB0471061.10 
41% TO CYP71B69 gi|255564492|ref|XP_002523242.1  Ricinus communis

EAAKEVFKTHDLDCCSRPCLPGAQRLTHNYLDIAFSPFNSFWIKLTDM

AFCIIVFIGPKLILKKKKKNHENHIARNCS*LSSSAALVNLSKKAYALIVNIAFTIAFGM

TFGRNGFDNDEVQ*TIQEAEA 

 

CYP71D

 

CYP71D subfamily (68 sequences, 22 are complete or nearly complete )

In Euphorbia lagascae DD025978 (patent) CYP726A1 is a Cytochrome P450 enzyme

associated with the synthesis of delta12-epoxy groups in fatty acids.

(vernolic acid) used for high temp lubricant

 

>CYP71D JcCA0123251.10 + runs off the end

BABX01002645

49% to CYP71BE1 Vitis vinifera
51% to CYP726   JcCA0077481.10
59% to XM_002522857 Ricinus communis
finished, no ESTs to extend
56% to CYP71D329  gi|255563804|ref|XP_002522903.1  Ricinus communis

MASVSFSPSKIRTLTISPTKPPITPSSTTVSLIHSSSSSLPFRLKIKCQATETDANKLPK

RLPPGPIKLPLIGNLHNLAGAQPHHALTELAKEYGPLMHLQLGEISAIVVSNPRIAQVIM

KTHDLVFADRPEILAAKIITYGGQDIAFSKLGEYWKQMKRISLMELLGPKTVQSFASLRE

NEVEKLIQSIHLSKGKPINFTEKIFHLTNVITCKAAFGDECKDQDVVIALTKEATTIAGG

FGIADVFPSMEFLQAITGVKGKLEKLRDELGDVFGNIIDEHKQKLMNRDGSDDVESEKED

LVDVLL 

 

>CYP71D329 XM_002522857 Ricinus communis, XP_002522903.1

MGALTLHPTTMVGFPTSTSVSLHFHPSPHPHRLKVRCQKTEADQ                      KVVKKLPPGPMKLPIIGNLHNLAGKLPHHALQELAKEYGPILHLQLGEVSTMVVSDGK                      MAKEVLKTHDLNFAQRPRLLAADIVLYDGKDIAFSPYGEYYKQMKKVGAEELLGPKRV                      QSYSSLREEEVQKLVESVRLSLGKPINFGDRICRLTNVIVFKAAFGEECKEQDTAIAV                      CVTATTLAGGFQIADVFPSLTFLHDINGFKTKVKEVAKEIDRMLSDIVEEHSKKLTSR                      EINNDGTEREDLVDVLLKLQRSGRFQCEVTTDHIKAVIFDFFIAGTETSSNTIEWAMS                      ELMRNPRVLKKAQAEVREAFKGKKTIRDADVKDLKYLELVIKETLRLHPPLPLLLPRE                      NKQSCAIGGYQIPAKTRMIVNAYAIGRDPKTWRDADKFIPERFLDAAVDFIGMDFEYI                      PFGGGRRICPGMNLGMANMQLPLAQLLYHFDWKLPDGIAPEDLDMTETFGATITRKNK                      LHVIPTRYQPLQGNQQREAIKVVN

 

>CYP71D JcCA0298871.10 + pseudogene fragment, N-TERM

BABX01022272.1

59% to JcCB0123521.10 
59% to CYP71BE6 Vitis
57% to CYP726A JcCA0309451.10
58% to CYP71AV1 
63% to CYP71D114 XM_003519575 Glycine max
no ESTs
N-term
Finished
57% TO CYP71D324 gi|255563520|ref|XP_002522762.1  Ricinus communis

4881 MELQLPSFWIIFFTFLLFLYMVIKLMNPFSSTKLPPGPRKLLIIGNLHQLKGLPHH 5048

5049 SLKKLAE 5069 &

5069 KKYGPIMHLKLGELKTIVLSSPEFAKQVFTTHDVIFANKLIARK (1?) 5200

 

>CYP71D JcCA0301341.10 - 

65% to CYP726A2 Euphorbia esula (Malpighiales)
66% to CYP71D338 gi|255544544|ref|XP_002513333.1  Ricinus communis
64% to CYP71D341   gi|255544556|ref|XP_002513339.1  Ricinus communis
complete

MEDQILSFQVLFSFLLFLLVLFKVSKKLYKHDSNPPPGPRKLPFFGNILQLAGDVPHRRL

TALAKTYGPVMGIKLGQIPFLVVSSPETAKEVMKIQDPVFAERALLVAVEIVLYNRNDIV

FGLYGDQWRQMRKICTLELLSAKRVQSFRSVREEEVADLVKFLGSKEGSPVNLTHTLFAL

ANSMIARNTVGHKSKNQEALLRLIDDIIESIGGVGIADIFPSLKWLPSVQRERSRIRKLH

YETDEILEDILQEHRANRQAAASRNGDQRGADNFLDVLLDLQQSGNLDVPLTDVAIKAAI

IDMFGAGSDTSSKTAEWAMAELMRNPEIMKKAQEELRNFFGENGKVDEAKLQELKWLYLI

NKETLRLHPAVAVVPRVCRERTKVSGYDVYPGTRVFINAWAIGRDPKVWSEAEKFKPERF

IDSAIDYKGTNFELIPFGAGKRICPGMTLGMANLEIFLANLLYHFDWKFPKGVTAENLDM

NEAFGAAVKRKVDLELVPIPYRP

 

>CYP71D JcCA0150621.10 -  pseudogene

BABX01005721 runs off end at ETAK

68% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
97% to CYP71D   JcCA0301341.10

ETAKEVMKIQDPVFAER &

ALLVAVEIVLYNRNDIIFGLYGDQWRQMRKICTLELLSAKRVQSFRSVREEEVADLVKF

LGSKEGSPVNLTHTLFALANSIIARNTVGHKSKNQEALLRLIDDIIESIGGVGIADIFPS

LKWLPSVQRERS*IRKLHYETDEILEDILQEHRANRQAAASRNGDQRGADNLLDVLLDLQ

QSGNLDVPLTDVAIKAAII (0)

DMFGAGRDTSSKTAEWAMAELMRNPEIMKKAQEELRNFFGENGKVDEAKLQELKWLNLIN

KETLMRLHPAVAVVPRICRERTKVSGYDVYPGTRVFINAWAIGRDPKVWSEAEKFKPERF

IDSAIDYRGTNFELIPFGAGKRICPGMTLGMANLEIFLANLLYHFDWKFPKGVTAENLDM

NEVFGGAVKRKVDLELVPIPFRP*

 

>CYP71D JcCA0019181.10 - 

64% to CYP726A2 Euphorbia esula (Malpighiales)
64% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
62% to CYP71D341   gi|255544556|ref|XP_002513339.1  Ricinus communis
complete

MEQQILSFPVLFSFLLFLLVLLKVSKKLSKHDSNSPPGPWKLPFLGNILQLAGDLPHRRI

TELAKKYGPVMSIKLGQHPYLVVSSPETAKEVMRTQDPIFADRPLVLAGELVLYNRNDIG

FGLYGDQWRQMRKFCALELLSTKRVQSFRSVREEEIAEFVKSLRSKEGSSVNLSHTLFAL

TNSIIARNTVGHKSKNQEALLKIIDDIVESLGGLSTVDIFPSLKWLPSVKRERSRIWKLH

CETDEILEGILEEHKANRQAAAFKNDDGSQADNLLDVLLDLQQNGNLEVPLTDVNIKAVIL (0)

GMFGAGSDTSSKTTEWAMAELMKNPEIMKKAQEELRSLFGESGYVDEAKLHEIKWLKLI

INETLRLHPAVTLIPRLCREKTKVSGYDVYPNTRVFINTWAIGRDPTIWSEPEKFVPERF

IDSSIDYRGNHFEYTPFGAGRRICPGMAFGMVNLEIFLANLLYHFDWKLPKGITSENLDM

TENFGGVIKRKQDLELIPAPFRP

 

>CYP71D JcCB0836941.10 +

BABX01108106 runs off the end  at RRIC

93% to CYP71D   JcCA0019181.10
65% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis

RRICPGMTFGMVNLEIFLANLLYHFDWKLPGRITSENLDMTENFGGVIKRKQDLELIPVPFRP*

 

>CYP71D JcCA0134061.10 +  N-term

98% to CYP71D JcCA0019181.10 allele?
60% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis

No ESTs to extend seq

MEQQILSFPVIFNFLLFLLVLLKVSKKLSKHDSNSPPGPWKLPFLGNFLQLAGDLPHRRI

TELAKKYGPVMSIKLGQHPYLVVSSPETAKEVMRTQDPIFADRPLVLAGELVLYNRNDIG

FGLYGDQWRQMRKFCALELLSTKRIQSFRSVREEEIAVFVKSLRSKEGSSVNLSHTLFAL

TNSIIARNTVGHKSKNQEALLKIIDDIVESLGGLSTVDIFPSLKWLPSVKRERSRIWKLH

CETDEILEGILEEHKANRQAAAFKNDDGSQADNLLDVLLDLQQNGNLQVPLTDVNIKAVIL (0)

 

>CYP71D JcCB0026731.10 +  

missing the N-term

65% to CYP726A2 Euphorbia esula (Malpighiales)
66% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
finished could not extend

EST GO247167

LLSTKRVQSFRPVREEEVASFVKLMRTKKGTPVNLTHALFALTNSIVARNAVGHKSKNQE

ALLEVIDDIVVSGGGVSIVDIFPSLQWLPTAKRERSRIWKLHQNTDEILEDILQEHRAKR

QATASKNWDRSEADNLLDVLLDLQQSGNLDVPLTDVAIKAAIIDMFGAGSDTSSKTAEWA

MAELMRNPEVMKKAQEELRNFFGENGKVEEAKLHELKWIKLIIKETLRLHPAVAVIPRVC

REKTKVYGYDVEPGTRVFINVWSIGRDPKVWSEAERFKPERFIDSAIDYRGLNFELIPFG

AGKRICPGMTLGMANLEIFLANLLYHFDWKFPKGVTAENLDMNEAFGGAVKRKVDLELIP

IPFRP* 

 

>CYP71D JcCA0290321.10 + 

61% to CYP726A2 Euphorbia esula (Malpighiales)
62% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
complete

MEHQILSFPALFSFLLFLLVLLKVSKKLYKHDSNPPPGPWKLPFLGNILQLAGDTFHRRL

TELAKTHGPVMSINVGQIPYVVVSSPETAKEVMKIQDPVFADHPVVLAAEVILYSPYDIF

FAPYGDHLKQMRKFCTVELLSTKRVQSFRSVREEEVADFVKFLRSKEGSSVNLTHTLFAL

TNSIVARTAVGHRSKNQEGLLKVIDEAVLASSGVNIADIFPSLQWLPSVKRERSRIWKTH

RETDKILEDVLQEHRANRKAAVPKNGDQSQADNLLDVLLDLQESGNLDVPLPDAAIKGTI

MEMFGAGSDTSSKTVEWAMAELMRNPEVMRKAQEELRSFFGENGEVEDAKIQELKCLKLI

IKETLRLHPPGAVIPRLCRERTKVAGYDIYPNTKIFVNTWAIGRDPEIWSEAEKFNPDRF

IDSSIDYKGNNFELIPFGAGRRICPGITLASANMELFLANLLYHFDWKFPQGITAENLDM

NECFGGAVKRKVDLELIPIPFRT

 

>CYP71D JcCA0141621.10 + 

59% to CYP726A2 Euphorbia esula (Malpighiales)
60% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis

GW619637

complete

MEQQILSVSVLSSFVLFLFVLLKVSKKLYKHDSNPPPGPWKLPFLGNILQLAGDAPHHRF

AELARTYGPVMGIKLGEIPFLVVSSPEAAKEVMKIQDPIFAERALVFANDVLNYNRNGMV

FGSYGYQWRQLRKFCTLALLSAKRVQSFQSVRKEEMADFVNFLRSKEGSSVNLTHTIFAF

TNSIIARNAVGHKTKNQETLLTCIDGIIYTGGVNIADVFPSLKWLPSVKREKSRVMKLHY

ETDKILEDILQEHKANKQAWVSEDGDGRKAGNFVDVLLDLQQSGNLDFPLTDVTIKASTI

DAFVGGSDTSSKTTEWAMAELMRKPEIMKKAQEELRSVFGEKGYIEEAKLQELKWLKLII

KETMRLHPVLSLLPRVCKQKTKVSGYDVYPGTQVLVNVWALGRDPKHWSEPEKFNPERFI

DSSIDYLGNHFEYLPFGAGKRVCPGIALGMVHMENFLANLLFHFDWKFPKGITAENLDMT

DAFGGVMKRKVDLELIPIPYHP

>JcCB0054381.10 +  pseudogene

BABX01089034.1

59% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
98% to CYP71D   JcCA0141621.10

MEQQILSVSVLFSFVLFLFVLLKVSKKLYKHDSNPPPGPWKLPFFGNILQLAGDASHHRF

AELARTYGPVMGIKLGEIPFLVVSSPEAAKEVMKIQDPIFAERALVFANDVLNYNRNGMV

FGSYGYQWRQLRKFCTLALLSAKRVQSFQSVREEEMADFVNFLRSKEGSSVNLTHTIFAF

TNSIIARNAVGHKTKNQETLLTCIDGIIYTGGVNIADVFPSLKWLPSVKREKSRVMKLHY

ETDKILEDILQEHKA

(large deletion)

IMKRKVDLELIPIPYHP*

 

>CYP71D JcCB0821811.10 -  

61% to CYP726A2 Euphorbia esula (Malpighiales)
60% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
missing N-term 39 aa

MELPFLGNILQLTGDDPHHRFAELARTYGPVMGIQLGEIPFLVVSSPEAAKEVLKIQDPI

FAERALILANDVVNYNRNGMVFASYGYQWRQLRKFCTLALLSAKRVQSFQSIREEEMADF

IKLLRSKEGSSVNLTHTLFTVTNSIIARNAIGHKSKNQEMLLRCIDGIIFTLGFNIADVF

PSLKWLPSVKREESRVLKLHHETDKILEDILQEHKANKQSWVSEDGDGRKADNFVDVLLD

LQQSGNLDFPLTDVTIKASTIDVFVGGSDTSSKTTEWAMAELMRKPEIMKKAQEELRSVF

GEKGYIEEAKLQELKWLKLIIKETLRLHPVLSLLPRVCKQKTKVSGYDVYPDTRVLVNVW

ALGRDPKIWSEPEKFNPERFIDSSIDYLGNHFEFLPFGGGKRICPGITLGMIHMEIFLAT

LLFHFDWKFPKGVTAENLDMTDAFGGVMRRKVDLELIPIPYHP

 

>CYP71D JcCB0665641.10 - 

BABX01097159 runs off the end at HPV

97% to CYP71D   JcCB0821811.10
61% to CYP71D339 XP_002513335.1  Ricinus communis

No ESTs to extend seq

HPVLSLLPRVCKQKTKVSGYDVYPDTRVLVNVWALGRDPKNWSEPEKFNPERFIDSSIDY

LGNHFEFLPFGGGKRICPGITLGVIHMEIFLATLLFHFDWKFPKGITAENLDMTDAFGGV

MRRKVALELIPIPYHP*

 

>CYP71D JcCA0163731.10 + 

95% to CYP726A JcCB0821811.10
60% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
runs of the end at PHH, missing 62 aa at N-term

PHHRFAELARTYG &

PVMGIQLGEIPFLVVSSLEAAKEVLKIQDPIFAERALILANDVVNY

NRNGMGFASYGYQWRQLRKFCTLALLSAKRVQSFQSIREEEMADFIKLLRSKEGSSVNLT

HTLFTVTNSIIARNAIGHKSKNQETLLRCIDGIIFTLGFNIADVFPSLKWLPSVKREESR

VLKLHHETDKILEDILQEHKANKQAWVSEDGDGRKADNFVDVLLDLQQSGNLDFPLTDVT

IKASTI (0)

YVFVGGSDTSSKTTEWAMAELMRKPEIMKKAQEELRSVFGEKGYIEEAKLQELKWLKLIIK

ETMRLHPVLSVLPRVCREKTKVSGYDVYPDTRVLVNVWALGRDPTVWSE

PEKFNPERFIDSSIDYLGNHFEYLPFGAGKRVCPGIALGMVHMENFLANLLFHF

DWKFPKGITAENLDMTDAFGGVMKRKVDLELIPIPYHP*

 

>CYP71D JcCD0004812.10 -  pseudogene

BABX01139558.1 runs off end at LHP

1 aa diff to CYP71D   JcCA0163731.10

ETLLRCIDGIIFTLGFNIADVFPSLKWLPSVKREESRVLKLHHETDKILEDILQEHKANK

QAWVSEDGDGRKADNFVDVLLDLQQSGNLDFPLTDVTIKASTI (0)

YVFVGGSDTSSKTTEWAMAELMRKPEIMKK &

AQEELRSVFGEKGYIEEA &

KLQELKWLKLIIKETLRLHP 

 

>CYP71D JcCB0303031.20 - 

56% to CYP726A2 Euphorbia esula (Malpighiales)
60% to CYP71D341   gi|255544556|ref|XP_002513339.1  Ricinus communis
complete

MDHRILSFPFLMLSLLLPFVFELLKIWKKSNNNPPPGPWRLPLIGNIHQLGGRHQPHLRL

TDLARTYGPVMRLQLGQIEAVVISSAETAKQVMKTQESQFLGRPSLLAADIMLYNRTDIS

FAPYGDYWRQMKKIAVVELLSAKRVQAYKSVMDEEVSNFINFLYSKAGSPVNLTKTFYSL

GNGIIAKTSIGKKFKKQETFLKVVDKAIRVAGGFSVGDAFPSFKLIHLITGISSTLHTAH

QEADEILEEIISEHRASKTADGDDYEADNILGVLLDIQERGNLQVPLTTDNIKAIILDMF

AGASDTSLTTAEWAMAEMVKHPRIMKKAQDEVRRTLNQEGNVANLLPELKYLKLVIKETL

RLHPPVALIPRECDGRCELNGYDVNPKTKILVNAWAIGRDHNLWNDPERFDPERFLDNSS

DFRGTDFKFIPFGAGKRICPGITMAITIIEVLLAQLLYHFDWKLPDGAKPESLDMSDTFG

LVVKRRIDLNLIPIP 

 

>CYP71D JcCA0309451.10 + 

54% to CYP726A2 Euphorbia esula (Malpighiales)
70% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
next best 61% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
complete

MEYLQQFSIFPAFFTFLLFIFILQRKLKTSSAKLAPGPWKLPIIGNMHQLLGFLPHHRLR

DLSNKYGPVMHLQLGQVSNIVISSPEAAKQVMKTHDIVFAQRPFLLAANIFMYNFKDIAF

APYGEGWRQMRKICTLELLSTKRVRSFRVIREEETSKFIRSIFSLSNVNISKMVFSLSNT

IALRSAFGRFCEHQDAFLPLVQKIVQMLEGFSVADIFPSVKFLHGITGMRSKLEKLHQEA

DIILENIIHEHRENKRLGSSSSQGKEDDLVDVLLNLQDQDNLEFPLTTENIKAVMLDIFL

AGTEPSATTIDWAMSEMLKDSRVMKKAQDEIRQVFNQKENIDETRLDELKYLKLVIKETL

RLHPPAPLLLPRECLETVKIDGYQVPINTKVIVNAWAIGRDSRHWTEAEKFYPERFQNNS

IDFRGKDFELLPFGAGRRMCPGIAYGMAVVELVLANLLFHFDWKLANGMEPHHLDMSECF

GASARRKNELHLIPIPYHVLPSK

 

>CYP71D JcCB0521021.10 +

84% to CYP71D JcCA0309451.10
62% to CYP71BE1   Vitis vinifera
63% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis 

MEYLQQFSIFSALFTFLLFIFILQRKPKTSKKLAPEPWKLPIIGNMHQLLGSLPHHRLRH

LSNQYGPVMHLKLGQISNIVISSPEAAKQVMKTHDIIFSQRPFLLAAEIIFYNFKDIALA

PYGDSWRQMRKICTLELLSTKRVRSFRAIREEETSNFIRSISS

(GAP)

LSEVNXKNKRLGRSNSH

GKEDDLVDVLLNLQCGDSLEFPLRIENIKAVMLDMFLAGTETSATVIEWAMSEMVKDSRV

MKKAQEEIRQVFNEKENIDETRFDELKYLKLVIKETLRLHPPAPLLAPRECLEAVEIDGY

EVPINTKVIINAWAIGRDSRHWNEAEKFYPERFQNNSIDFKGNDFEFIPFGAGRRMCPGV

TYGLAVVELVLANLLYHFDWKLPNGLEAHQLDMSETFGATARRKNELHLIPIPCNTSLLET

 

>CYP71D JcCA0116831.10 + 

52% to CYP726A2 Euphorbia esula (Malpighiales)
68% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
complete

MEYLQQLPIFPALFTFLLFIFILQRISTNYSAKLAPGPWKLPIIGNMHQLLGSIPHHRLR

HLSNQYGPVMHLKLGQISNIVISSPEAAKQVMKTHDIIFSQRPFLLAAEIIFYNFKDIAL

APYGDSWRQMRKICTLELLSTKRVRSFRAIREEETSNFIRSISSLSEVNISKMVLSLSNA

ITLRSAFGKVSERHEAFLPLVQKIVLVLEGFSVADIFPSVKFLHGITGMRSKMEKLHQEA

DIMLENIINEHRENKRLGRSNSQGKEDDLVDVLLNLQCGDSLEFPLTIENIKAVMLDMFL

AGTETSATVIEWAMSEMVKDSRVMKKAQEEIRQVFNQKENIDETRFDELKYLKLVIKETL

RLHPPAPLLAPRECLEAVEIDGYEVPINTKVIINAWAIGRDSRHWNEAEKFYPERFQNNF

IDFKGNDFEFIPFGAGRRMCPGVTYSMAVVELVLANLLYHFHWKLPNGLEAHQLDMSETF

GATARRKNELHLIPIPCSTSLLET

 

>JcCA0296221.20 – N-term pseudogene

BABX01021978.1

83% to CYP71D JcCA0116831.10

59% to CYP71D330 XP_002530922.1 Ricinus communis

WTFLLFIFILQRKPKTSTK &

KLAPRPWKLPVIGNMHQLLGSLPHRRLRHLSDKYGPVMHLKLA &

QVSNIVVSSPEAAKQVMKTHNIIFSQRPFLLAAEILIYNFKDIAFAPYGDSWRQMRKICT

LELLSTKRVRSFR &

EETSNFIRSISNLSEVNISKMVLCLSNAITLRSAFGKASECNEAFLSLVQKIVEGFSVAD

FPFVKFLHGITGMKSKLEKLYQEADIMLEIIINEHRENKRLGRSNSEGKEEDLMDVLLNL

QYGDSFEF &

PLTLENRKAVML (0)

 

>CYP71D JcCB0508101.10 +  pseudogene

BABX01086843.1

86% to CYP71D   JcCA0116831.10
59% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis

LAAEILMYNFKDIAFAPYGGSWRQMRKICML*LLSTKCVRSFRAIREEETSNFIRSISSL

SEVNISKMVLSLSNAITLRSAFRK &

LFERHEAFFPPVQKIVQVFEGFSVADIFPAVKFLHRITGIRSKLETLHQGADIMLEN

IINEQREN*RLGRSNSEGSEDDLVDVLLNFQSGDSFEFPLTIENIKAVML (0)

DIFLDGTETSATVIEWAMSEM

 

>CYP71D JcCD0001888.10 –

BABX01136955.1 

96% to JcCB0040361.20, 96% to CYP726   JcCA0116831.10
69% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
no ESTs to extend seq.

FRAIREEETSNFIRSISSLSEVNISK

MVLSLSNAITLRSAFGKVSERHEAFLPLVXKIVLVLEGFSVADIFPSVKFLHGITGMRSK

MEKLHQEADIMLENIINEHRENKRLGRSNSQGKEDDLVDVLLNLQCGDSLEFPLTIENIK

AVMLDMFXAGTETSATTIEWAMSEMVKDSRVMKKAQEEIRQVFNQKENIDETRLDELKYL

KLVIKETLRLHPPAPLLXPRECLEAVEIDGYEVPINTKVIINAWAIGRDSRHWNEAEKFY

PERFQNNSIDFKGNDFEFIPFGAGRRMCPGLTYGLAVVELVLANLLYHFDWKLPNGLEAH

QLDMSETFGATDRRKNELHLIPIPCNTSLLET

 

>CYP71D JcCB0442001.10 +

BABX01082196.1

87% to CYP71D   JcCD0001888.10
71% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis

LSLSNAITLRSAFGKVSERQEAFLPLVQKIALMLEGFSIADIFPSVKFLHGITGMRSKLQ

KLHQEADIMLENIINEHRENKRLGRRNSEGKEDDLVDVLLNLQDHDNLELTTENMKAVML (0)

DMFLGGTESSSTVIEWAMSEMVKDSRVMEKAQEEVRQVYNQKENIDETRLDELKYLKLII

KETLRLHPPAPLLIPRESLEAVEIDGYEVPINTKVII &

INAWAIGRDSRHWTEAEKFYPERFQNNSIDFKGNDFQFIPFGAGRRMCPGIAYGMAVVEL

VLANLLFHFDWKLPNGLEPHLLDMSESFGVSARRKNELNLIPIPYNPS

 

>CYP71D JcCB0021881.20 - 

BABX01067281.1

61% to CYP71BE1   Vitis vinifera
89% to CYP71D   JcCD0001888.10
69% TO CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
NO ESTS

NISKMVLSLSNAITLRSAFGKVSERHEAFLPLVQKLTLVFEGFSVADLFPSVKFL &

HGITGMRSKLENLHKEADIMLENIINEHRENKRLGRSNSQGKEDDLVDVLLNLQNCDNLEFPLTI

ENIKAVML (0)

DMFAAGTETSATTIEWAMSEMVKDSRVMKKAQEEIRQVFNQKGNIDETRLYELKYL

KLVIKETLRLHPPAPLLLPRECLEAVEIDGYQVPINSRVIINAWAIARDDRYWNEAEKFY

PERFQNNSIDFKGNDFEFIPFGAGRRMCPGLASGMAVVKLALANLLYHFDWKLPNGIEPH

QLDMSESFGLTARRKNELHLITIPHNTSELEI*

 

>CYP71D JcCB0040361.20 +

BABX01079772.1

95% to CYP726 JcCA0116831.10
67% TO CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis 
no ESTs to extend seq.

FRAIREEETSNFIRSISSLSEVNISKMVLSLSNAITLRSAFGKVSERHEAFLPLVHKIVLVL

DGFSAADIFPSVKFLHGIIGMRSKIEKLHQEADIMLENIINEHRENKRLGRSNSHGKEDD

LVDVLLNLQCGDSLEFPLSIENIKAVMLDMFLAGTETSATVIEWAMSEMVKDSRVMKKAQ

EEIRQVFNQKENIDETRFDELKYLKLVIKETLRLHPPAPLLAPRECLEAVEIDGYEVPIN

TKVIINAWAIGRDSRHWNEAEKFYPERFQNNSIDFKGNDFEFIPFGSGRRMCPGLTYGLA

VVELVLANLLYHFDWKLPNGLEAHQLDMSETFGATDRRKNELHLIPIPCNTSLLET

 

>CYP71D JcCB0166851.10 +  

52% to CYP726A2 Euphorbia esula (Malpighiales)
66% TO CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
complete

MEYMPEFSIFSALFTFLLFVFILQRKPKTSSHKLVPGPWKLPIIGNMHQLFGS

LPQHRLRDLSKKYGPVMHIKLGQVSNIVVSSPEAAKQVMKTHDIIFLQRPFLLAAEILMY

NFKDIAFAPYGDSWRQMRKICTLELLSTKRVRSFRPIREDEVSTFIRTISSSSKVNLGRM

VFALSNTITLRSAFGKVSERKEAFLPLVQKIVQVLEGFSVADVFPSVRFLHRITGMRGKL

EKLHQETDIMLENIINEHRENKRLGRSNSEGKEDDLVDVLLNIQDSDNLEFPLTMEHIKA

VMLDMFLGGTETSAATIEWAMAEMVKDPRVLEKAQKEVRQVFNHKENIIDETRLDELKYL

KLVIKETLRLHPPVPLLVPRQSLDAVEIDGYKLPINTKVIINAWALGRDSRHWNEAEKFY

PERFQNNSIDFKGNDFQFIPFGAGRRMCPGVGYGMALVELALANLLYHFDWKLPNGLEPH

LLDMSDSFGASARRKHELHLIPIPYNSSPSPVK

 

>CYP71D JcCB0175211.10 - 

50% to CYP726A2 Euphorbia esula (Malpighiales)
64% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
complete

MENQFPSFPFIFTFLVFLFMVLRLWKNSKKNSAPNLPPGPWKLPVIGSMHHLSGSLLPHV

RLRNLANEYGPLMHLKLGEVTNIVVSSPETAKAIMKTHDHIFAQRPFLLAANIMAYNSTD

LAFAPYGDYWRQMRKICTQEILSAKRVLSFGLIREEEVSKFIRDLSSRAGSTVNFSRMFN

SVTYNIIQRVAIGKLWKGEEIVIPAFKKLIEAAGGFSLSDLYPSIKLLHKISTTKFKLLR

AHKETDKLFQNIIDEHRARKASRAKSGAKNEEEDIIDVLLQAQSEEELEYPITDDNIKAV

IMDVLSGGTDTSATTVVWAMSELLKNPDVMKRVQTEVRQVFSKKGYVDEESIGELHYLKA

VVKETMRLHPTGAVLTRECREDCVINGYDIPYKSRIIINAWALGRDPDYWPEAERFNPDR

FLNSSIDYKGKHFEFLPFGAGRRICPGILFGISNVQFPLARLLYHFDWKLPNGMRPEDLD

MNEKYGIAVRRANDLQLIPIPCFPPQPQVK

 

>CYP71D JcCB0077881.20 +  N-term

BABX01105098.1 RUNS OFF THE END AT MHH

70% to JcCB0123521.10
69% to CYP726   JcCB0175211.10
69% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis

MEQENQILYFPALFTFLLFLFFLLRVWKKFKTNNSTPNLPPGPWKLPLIGSMHH

 

>CYP71D JcCA0316031.20 –

61% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis 
50% to CYP726A2 Euphorbia esula (Malpighiales)
complete

MLFFITVFFIFIALRIWKKSKSNSTLNLPPGPKKLPLIGNIHNLAGYLPYHRLRDLSNEY

GPIMHLQLGEINSIVVSSPELAKEVMKTHDINFAYRPFVLAGDIVSYKCKDIAFAPYGEY

WRQLRKMCSLELLTAKRVQSFKSIREEEGSKLLQSISSSSGSPVNFSKMTSSLTYSIISR

AAFGKVCQGEEVFVPAVVKLTEAGRSISLADVYPSVKLFNTFSVVRRNVEKIHSEVDKIV

ENIVKEHKERKRVEDIGMKSKEEEDLVDVLLKFQENGDVDSSLSDESVKAVILDMFIAGS

DTSSTTLEWAMSEMMKNPSIMEKAQAEVRKVFGSKGKVDEAGLHELSYLKLVIKETLRLH

PPVPLLLPRENKENVVIEGYDIPAKSKVVVNAWAIARDPKYWDEAERFYPERFINSSIDF

KGANFEFIPFGAGRRMCPGMIFGLASVELPLAQLLYHFDWKLPGGQKPEDLDMSEDLGGT

ATRSNALYLIPTPYIPPTVGKISR

 

>CYP71D JcCB0054631.10 + 

51% to CYP726A2 Euphorbia esula (Malpighiales)
65% TO CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
complete
EST GW615837

MEYLQQLSIFSALFTFLLFIFILQRKHKTSSKKLAPGPWKLPVIGNMHQLLGCLPHHRLR

HLSNKYGPVMHLKLGQVPIIVVSSPEAAKQVLKTHDIVFSQRPFLLAAEILMYNFKGIFS

APHGHSWRQMRKICALELLSTKRVRSFRAIREEEVSNFIRSISSLLKVNISKMVSSLSNT

IALRSAFGKVLERHEAFWPLVQKIMQVLEVSSVADLFPSVKFLHRITGMSSKLEKLHQET

DIMLENIISEHRENKRLGRSNSQGKQDDLVDVLLNIQDSDNLELPLTIENIKAVMLDMFL

GGTETSATLIEWAMSEMVKDSRVMRKAQEEIRQVYYQKENIDETRLDELKYLKLVVKETL

RLHPPGPLLVKRECLEAVEIDGYEVPINTKVNVNAWAIGRNSRYWIEAEKFFPERFQNNS

IDFKGNDFEFIPFGAGRRMCPGIAYGMAVVELVIANLLYHFDWEQPNGIEPHHLDMSESF

GITARRKNELHLIPIPYNPFPSQVS 

 

>CYP71D JcCB0420261.10 -  

48% to CYP726A2 Euphorbia esula (Malpighiales)
60% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
missing about 9 a at N-term

FPTLFAFLLFLFMVLTLWRKTKSKPNLPPGPWKLPLIGSMHHLAGPSLLHHRVTELARKY

GPIMHLQLGQVTNIFISSPEIAREVMKTHDLIFATRPSLVAVQLVTYNFTDIAFAPYGDY

WRQIKKICTMELLTAKRVQLFAPIRQEEVSKVITDITSNVGSTINFTNVLTSLTYKILSR

STIGKILKGEEGFIRAVMDLTEEGAGFNLADFYPSIKLFRMFGSLKHKLKRIHQQVDKMM

QNVIDDRRATKRESGVDDEERDIVDVLLRIQEQGDLQLPLTDDNIKAVIFDMFSAGSDSS

AATTIWTMSELLKNPSVMEKAQAEVRQVFKKKGQVDEEGMEELHYLKAAVKETLRLHAPG

PLVVPRECTENCVIAGYDIPAKSRINVNSWALGRDPEYWTEPERYSPERFLDGSIDHKGN

NFTYLPFGSGRRLCPGILFGLTNIEFILANLLYYFDWKLPNGLQPEDLDMTEVFGSAARR

KNDLLVIPFQYLPPAVN

 

>CYP71D JcCA0313091.10 + 

49% to CYP726A2 Euphorbia esula (Malpighiales)
63% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
complete

MESQFLSFPFLFTFLVLLLTVLRLWKKSRKNSAPNLPPGPQKLPIVGSMHHLSGSSLPHV

RLRDLAKEHGPIMHLQLGEVTNIVVSSPETAKAVMKTHDHIFAQRPFLLAANIMAYNSTD

LAFAPYGDYWRQMRKICTQEMLSNKRVQSFGLIREEEVSKLITELSSRAGSTVNFSKMFN

SVTYNIIQRVAIGKLWNGEEVVIPAIKKLIEAAGGFSLSDVYPSIKLLHKISTTRFKLQR

AHKEADRVFQNIIDEHRARRASRAKSGAKNEEEDLIDVLLQAQSKEDLEHPITDDNIKAV

ILDVLSGGSDTSANTVVWVMSELQKNPDVMKRVQTEVRQVFSEKGYVDEESIGELHYLKA

VVKETMRLRPPGTILTRECMADCVINGYDIPHKSRVIINAWALGRDPDYWPEAERFNPDR

FLNSSIDFKGNHFEFLPFGAGRRVCPGILFGISNVQFPLARLLYHFDWKLPNGMQPEDLD

MNAKYGIAVTRVNDLQLIPIPYLPAKVK

 

>CYP71D JcCB0625131.10 –

BABX01094844   runs off the end at MGSW

97% to CYP71D   JcCA0313091.10

RDPDYWPEAERFNPDRLLNSSIDYKGNHFEFLPFGAGRRVCPGILFGISNVQFPLA

RLLYHFDWKLPNGMQPEDLDMNAKYGIAVTRANDLQLIPIPYLPAKVK*

 

>CYP71D JcCA0267751.10 - 

49% to CYP726A2 Euphorbia esula (Malpighiales)
63% TO CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
complete

MLFFITVLFIFIALRIWKKSKANSTPNLPPGPNKLPLIGNVHNLVGDLPYHRLRDLSKKY

GPIMHLQLGENTTVVISSPELAQEVMKTHDVNFAQRPFVLAGDIVSYKCKDIAFAPYGEY

WRQLRKMCSLELLTAKRVQSFKSIREEEVSKLVESISSSSGSPINFSKMASSLTYAIISR

AVCGKVSRGEEVFVPAVEKLVEAGRSISLADLYPSVKLFNALSVVRRRVEKIHGEVDKII

ENIVIEHRERKRMAHAGINSKEEEDLVDVLLKFQENGDLDSYLSNDGIKAVILDMFIAGS

DTSSTTIEWAISEMVKNPSIMEKAQAEVREVFGSKGKVDEADLHELNYLKLVIKETLRLH

PAVPLLLPRQSREDCVIEGYNIATKSTVIVNAWAIARDPKYWDEAERFYPERFINSSIDF

KGTNFEFIPFGAGRRMCPGMLFGLASVELPLAQLLYHFDWKLPGGQKPEDLDMSDDLDGT

ATRRHALYLTATPYLPSAVGKI

 

>CYP71D JcCB0166761.10 + 

52% to CYP726A2 Euphorbia esula (Malpighiales)
68% TO CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis
BABX01063925.1 frameshift at & possible pseudogene
complete

MEYLQLFSVFSALFTFFLFIFILQRKPKTSSRKLAPEPWKLPIIGNMHQLLGSLPHHRLR

HLSNKYGPVMHLRLGEVSEIVISSPEAAKKVMKTHDIIFAQRPYLLAADIILYNFKDIVF

APYGEGWRQMRKICTLQLLSTKRVRSFRVVREEETSKFIRSISGLPKVNISKMVFSLSNA

ITLKSAFGKVSERHDAFLPLVQKIMLVFGGFSVADFFPSVKFLHRITGMRSKLEKLHQEA

DIMLENIINEHRENKRLGRSNSEGKEDVLVDVLLNLQNCDNLEFPLTMENIKAVML (?)

DMFVAGTETSATTIEWAMSEMVKDSRG &

KKAQEEIRQVYNQKENIDETRLDELKYLKLVIKETLRLHPPAPLLLPRECLEAVEIDGYQVP

INTKVIINAWAIGRDSRYWNEAEKFYPERFLNNSIDFKGKEFEFIPFGAGRRMCPGMAYG

MAVVELAVANLLYHFDWKLPSGIEPHQLDMSESSGVTARRKNELHLMPIPYNPFPSQVC 

 

>CYP71D JcCB0683541.10 +

BABX01098116.1  runs off the end at EID

96% to CYP71D JcCB0166761.10
78% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis

No ESTs to extend seq

EIDGYQVPINTKVIINAWAIGRDSRYWNEAEKFYPERFLNNSIDFKGKEFEFIPFGAGRR

MCPGIAYGMAVVELAVANLLYHFDWKLPSGIEPHQLDMSESFGLAARRKNELHLIPIPYN

PFPSQVC* 

 

>CYP71D JcCB0257541.10 + pseudogene

BABX01069864.1 RUNS OFF THE CONTIG AT LEN

59% to CYP71BE1   Vitis vinifera
77% to CYP71D   JcCB0166761.10
83% TO CYP71D   JcCB0040361.20
67% to CYP71D330 XP_002530922.1  Ricinus communis

LENIINEHKENKRLGKSNSEGKEDDLVYVLLNLQDNGNLEIPLTTEHIKADML (0)

DMFLAGTETSATVIDWAMSEMLKDSRVMKK &

KQDEIRQV &

YNQKENIDETRFDELKYLKLVIKETLRLHPAAPLLDPRECLEAVEIDGYEVPI

NTKIIINRWAIVRDTRYWTEAEKFYPERFLNNSIDFRGKDFEF &

IPYGAGKRICPGITYGMAVVELVLANLLYHFDWKLPNGMKLHQLDMSEAFGAT

ARIKNELHLIPIPYNTSPSQ*

 

>JcCA0011081.20 +  N-term exon 1

BABX01001223.1

90% to JcCB0166761.10

61% to CYP71D330   gi|255580182|ref|XP_002530922.1  Ricinus communis

no EST to extend seq

MEHLLQSSIFPALFTFLLFNFILKRKLKTSSRKLAPGPWKLPIIGNMHQLLGSLLHHRLR

HLSNKYRTVMHLKLGQVSQIVISSPEAAKKVMKTHDIIFAQRPYLLAADIILYNFKDIAF

APYGEGWRQMRKICTLQLLSTKRVRSFRVVREEETSKFIRSIYGLPKVNISKMVFSLSNA

ITLKSAFGKVSERHDAFLPLVQKSVLVFGGFSVADIFPSVKFLNRITGMRSKLEKLHQEA

DIMLENIINEHKENKRLGRSNSEGKEDDLVDVLLNLQNCDDLGFPLTMENIKAVML (0)

 

>CYP71D JcCA0304601.10 - 

50% to CYP726A2 Euphorbia esula (Malpighiales)
64% to CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
complete
note: same as JcCD0062548.10 but JcCD0062548.10 has the correct C-term seq (join)

JcCD0062548.10 - 

BABX01141191 runs off contig at NIID

61% to CYP71D324 gi|255563520|ref|XP_002522762.1  Ricinus communis
exon 1 100% to    JcCA0304601.10

MENQFPSFPFLFTFLVFLFMVLRLWQKSKNNSALNLPPGPWKLPLIGSLHHLFGSVLPHT

RLRDLANEYGPIMHLQLGQVTNIVLSSPETAKAVLKTHDHIFTQRPFVLAAETMTYNFTN

LANAPYGGYWRQIRKICTLEMLSAKRVRSFGLIREEEVSKFIRDLSSSTSAGSTVNFSRM

FSSVTYNIIQRVAIGKISKGEDTVFPAIRKLIEAFVGFNLSDAYPSIKLLHKISTKRFKL

ERAHKEADKILQNIIDEHRARKASAANSEEEEDLVDILLNAQCQEDLQITDDNIKAIIL (0)

DVFSGGNDPSANTVLWAMSELIRNPEVMKKAQTEVRQVFSEKGYVEEESIGELYYLKAVVK

ETLRLHPPGTFLARECMEDCVINGYDISVKSIFVINTWALGRHPDYWPEAERFNPDRFLN

CSIDYKGNNFEFLPFGAGRRMCPGILFGISNVQFPLARLLYHFDWKLPNGMQPEDLDMN

AKYGIAVTRANDLQLIPIPYLPAKVK*

 

>CYP71D JcCA0077481.10 - 

50% to CYP726A2 Euphorbia esula (Malpighiales)
58% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis

BABX01029652.1

complete

N-term does not match very well,

Better top

CYP71D329   gi|255563804|ref|XP_002522903.1  Ricinus communis

MVVLNFTPLKMGPPTFVSLPHRSPSQLPFKVSCQKSEADNGKAIKKL

PPGPKKLPFIGNL

HNLGGSLPHHVLKDLANQYGPLMHLQLGEVPAIVISSSKMAQEVLRTQDHVFAQRPELLA

SKILSYDSTDLVFAKGEYWKQMRKFCFNELLGAKKVKSLAPLREDEVSNLLESIRSAGGS

PVNLTEKIFWMTSVIAVRAAFGNKWEDQKTIISIARESLSLAGGFDLADLYPEREFLHII

TNMKPRLEKMRVKLDGVLDRIVNEHKEKLLSGQTQPDNEVLVDVLLRFQETGNLRCPVTI

DNVKAVIWDMFVAGTDTSSTTTEWALSEMIRNPKVMKKAQEEVRQAAKGKTTIDEEDIQD

LPYLKQVIKETLRLHPPVPLLLPRESNQDSEIEGYYIPERTKVIVNAWAIGRDPAYWEDP

ESFIPERFSDSSIDFKGNNFTYIPFGAGRRICPGMTFGLANVELPLAKLLYHFDFKLPDG

IRGEDLDMTEAFGATVGRKNQLTVIATPYVPRNNESSSKHKETIEAFK

 

>CYP71D JcCA0137661.20 –

BABX01139730.1

missing the N-term

52% to CYP726A2 Euphorbia esula (Malpighiales)
61% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
complete

MENQFPSFPMLLAAFFFILITLILSEKSKTKNLPP

GPRKLPIIGNLHLFSSSYPIHHRLRDLSKKYGAV

MHLKLGQVSTIVIGSPEAAKEMMKANDVCFADRPCYQSAEIVTYNFLDIAYSPYEDYWRQ

LRKICTVELLSAKRVQSFRSIKEEEVANLIRDISSSSGKPFNLSKRIFALTYSITARVSF

GDKCREQDAFISAAEKIMQTTGFDLADLFPSFKFLGWFSEMRNRLMNAHDEADRIIESII

NDHRTNKKTTETEDIVDVLLKLQDNGNLQFPLTNTNIKAVILDLFVAGSETSSTTVEWAM

SEVLKNPRILSKAQEELRRIFDKKGKVDGEGLQESHYLKLVIKETLRMHPPAPLLLPREC

RESCQLNGYNIPAKTKVIVNGWALARDPNSWTDPDTFYPERFLDSTVDFKGNNYEFIPFG

AGRRICPGISFATPNIELPLANFLYHFDWQLPGGMKLENLDMSDGDFGTTITRRNDLVLI

PVPYHPPTMEA

 

>JcCA0149291.10 + pseudogene

BABX01005558.1

87% to CYP726   JcCA0137661.20
54% to CYP71BE1   Vitis vinifera
62% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis

QFPSFPMLLAALFFILILLILSEKSKTKNLPP GPRKLPIIGDLHLFSTSYPIHNRLRDLSKKYGGVMYLKLGQVPTIVIGTPEAAKEMMKTN DVCFADRPNPQSSALVTYNFLDIAYAPYGDYWRQLRKICIVELLSTKRVQSFRSIREEEV ANRIRDISSSSGKPFNLSKRIFALTYGITAR &

VSFGDICREQNEFISAAEKIVQNTGFDIAD LFPSLKFLGWFSEMRTRLMNARDEADRVIESIINDHRANKKTTGETDDIVNVLLKLQDNG

NLQFPLTNTNIKAVIL (0)

DLFVAGSETSSTTVEWAMSEVLKNPRILSKAQEELRRIFDKKGKVDEEGLQESHYLKLVIKETLRM HPPGPLLLPRECRESCQLNGYNIPAKTKVIVNAWALARDPNYWTEPDTFYPERFLDSTVD FKGNNYEFIPFGAGRRICPGISFATPNMELPLANFLYHFDWQLPGGMKLENLDMRDGDFG

STITRRNDLVLIPVPYHPPTMEA*

 

>CYP71D JcCD0049678.10 + 

BABX01139730

nearly identical (3 aa diffs) to JcCA0137661.20 up to SVELK

98% to CYP71D   JcCA0137661.20 whole seq
92% to JcCA0149291.10
62% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
complete
MENQFPSFPMLLAAFFFILITLILSEKSKTKNLPP GPRKLPIIGNLHLFSSSYPIHHRLRDLSKKYGAVMHLKLGQVSTIVIGSPEAAKEMMKAN DVCFADRPCYQSAEIVTYNFLDIAYSPYEDYWRQLRKICTVELLSAKRVQSFRSIKEEEV ANLIRDISSSSGKPFNLSKRIFALTYSITARVSFGDKCREQDAFISAAEKIMQTTGFDLA DLFPSLKFLGWFSEMRTRLMNAHDEADRIIESIINDHRANKKTTETEDIVDVLLKLQDNG 
NLQFPLTNTNIKAVIL (0)

DLFVAGSETSSTTVEWAMSEVLK &

NPRILSKAQEELRRIFDKKGKVDGEGLQESHYLKLVIKETLRMH PPAPLLLPRECRESCQLNGYNIPAKTKVIVNGWALARDPNYWTEPDTFYPERFLDSTVDF KGNNYEFIPFGAGRRICPGISFATPNXELPLANFLYHFDWQLPGGMKLENLDMSDGDFGT

TITRRNDLVLIPVPYHPPTMEA*

 

>CYP71D JcCB0542951.10 –

BABX01088977.1

GW876473.1 GW879708
51% to CYP726A2 Euphorbia esula (Malpighiales)
62% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
complete

JMS27L13r.10 - 

100% to CYP726   JcCB0542951.10 

MEEFHFNSLHSLFALFFFIIFFFKAIKK

RATKPSTTNLPPGPWKLTIIGNVHQLLGSLPHQSLQKLSGK

YGPLMHLKLGEVSTVIVSSPEIAKQVLKTHDLDFAERPPNLAP

KIISYDSTNIVFSPYGAYWRQLRKICTMELLSPKRVQSFRFIREDEVLNLIKTISSLEGS

LINISEMIFSLTYGITSRAAFGKKYEDQETFIQVITEVSKIAAGFSVADLYPSIKFLEQA

SGLRPKLGKLHEKADGILERIVKEHRNKMNRSEEIEEDDDLVDVLLKLQEHGDLEFPLSD

DNIKTVILDMFSAGSETSSTTVEWAMSEMLKNPRILEKAQNEVRQVYKNKGTVDETSIHE

LKYLNSIIKETLRLHPPLPLIPRESRARVEIIGYDIPIKTKVLVNAWAIGRDPKNWTEPE

NFCPERFLDNAISYKGTDFEFIPFGSGRRICPGISFALPNIELPLAQLLYHFDWKLGNGM

KNEDLDMTEGYGLTIRRKQDLFLVPMYPVNSEINRSSN

 

>CYP71D JcCA0151631.20 +  runs off the end

BABX01005828

64% to CYP726 JcCA0137661.20, No ESTs to extend the seq
77% to CYP71D326 XP_002522721.1  Ricinus communis

MSIFKMDLQQFLSLPILFTSFFFIFMVLKMWRKSKTKEATKNLPPGPRKLPIIGNIHQLI

GSLPHHCLRDLAKKHGGIMHLQLGEVSNIVISSPEAAKEVMKTHDIVFAQRPFLLAASII

SYNFTDIAFSPYADYWRQLRKICILELLSAKRVQSFRFIREEEVSNLITAISSSSGKAFN

FSRKLFSLTYGIAARATFGEKCEDQEEFIPIVEEITEVAGGFSLADLFPSVKFLHSISGM

RSRLIRLQKEADRVIGNIIDDHRAKNKTGKVGGEGQDDDLVDVLLRLQEHGNLEFPLTTD

NIKAVILDIFVAGSETSSTTVEWAMSEMLRNPRVMYKAQEEVRKLFDKKGNVEETDLQES

KYLKLVIKETLRLHPPAPLLL

 

>CYP71D JcCA0241141.10 - 

BABX01015828.1 runs off the end at KST

50% to CYP71BE1   Vitis vinifera
86% to CYP726   JcCB0175211.10
63% to CYP71D324 XP_002522762.1  Ricinus communis
no ESTs to extend seq.

MESQFLSFPFLFTFLVLLLTVLRLWKKWGKNSALNLPPGPWKLPIMGSMHHLSGSSLPHV

RLRDLAKEHGPIMHLQLGEVTNIVVSSPETAKAVMKTHDHIFAQRPFLLAANIMAYNSTD

LAFAPYGDYWRQMRKICTQEMLSNKRVQSFGLIREEEVSKLIAELSSRAGSTVNFSKMFN

SVTYNIIQRVAIGKLWKGEEVVIPAIKKLIEASGGFSLSDVYPSIKLLHKISTTKFKLQR

AHKEADRIFQNIIDEHRARRASRAKSGAKNEEEDLIDVLLQAQSKEDLEFPITDDNIKAV

ILDVLSGGSDTSANTVVWVMSELXKKSRRDEKST

 

>CYP71D JcCB0254461.20 – exon 1

BABX01069700.1

62% to CYP71D338 XP_002513333.1  Ricinus communis
94% to CYP71D JcCA0301341.10
no ESTs

MEDQILSFQVLFSFLLFLLVLFKISKKLYKHGSNPPPGPRKLPFLGNILQLAGDVPHRRL

TALAKTYGPIMGIKLGQIPFLVVSSPETAKEVMKIQDPVFAERALLLAGEIVLYNRNDII

FGLYGDQWRQMRKICTLELLSAKRVQSFRSVREEEVADLVKFLGSKEGSPVNLTHTLFAL

TNSITARNTVGHKSKNQEALLRLIDDIIESTGGVSISDIFPSLKWLPSVQRERSRIRKLH

YETDEILEDILQEHRANRQAAASRKGDRRGADNLLDVLLDLQESGNLDVPLTDVAIKAAII (0)

 

>JcCD0049428.10 + N-term

BABX01139697.1

96% to CYP71D JcCB0254461.20
63% to CYP71D338 XP_002513333.1  Ricinus communis

No ESTs to extend seq

MEDQILSFQVLFSFLLFLLVLFKISKKLYKHGSNPPPGPRKLPFLGNILQLAGDVPHRRLTALAKTYGPV

MGIKLGQIPFLVVSSPETAKEVMKIQDPVFAERALLLAGEIVLYNRNDIVFGLYGDQWRQ

MRKICTLELLSAKRVQSFRSVREEEVADLVKFLGSKEGSPVNLTHTLFALANSXIARNTV

GXKSKNQEALLRLIDDIIESIGGVGIADIFPSLKWLPSVQRXRSRIRKLHYETDEILEDI

LQEHRANRQAAASRNGDRRGADNLLDVLLDLQESGNLDVPLTDVAIKAAII (0)

 

>CYP71D JcCB0152991.10 - pseudo

BABX01063057

69% to CYP71D   JcCB0420261.10

CYP71D324 XP_002522762.1  Ricinus communis

No start Met seen

THYQYFFPTLFALLIFLFTVLRIWRKTKSKPNLPPGPWKLPLIGSLHHLAGAPLTHVRLR

DLAKKYGPIMHLQLGEVTTIFITSPEIAKEVLKTLDIVLARRPFLQAVKLVTYNFTDVAFSPYGEYWRQ

LRKICTMELLTAKRVQSFGSIRQEEGSKLIRDISSNAGSPINFSKILTSSGYKIISRAAF

GQVWNGEDVFLKAVNDLTEESAGFSLVDFYPSKKFLQLFTSSGQKLQRVFQQVDTIMQNI

IDNHRARKREAKSGDDAELEDFVDVLLKVQEQKDLELPLTDDNMKAVIFDMFSAGSDSSS

TTIIWAMSELVKNPTVMEKAQAEVRRVFSKKGRVDEEGIEELHYLKAVAKETLRLHAPGP

LLLPRECAENCVIND &

FDIPAKSRIAVNFWAIGRDPQYWTEPERFNPERFLDCPVDYNYKGTNYTYLPFGSGRRIC

PGMSFAIANMEFTLAQMLFYFDWKLPNGLPGESLDMTEKFGVTVRRESDLILIPFPYIPASVN*

 

>CYP71D JcCB0176751.10 - 

BABX01064550

61% t o CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
60% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
62% to CYP71D   JcCA0316031.20
EST GT969884 joins exon 1 and exon 2
Exon 2 = JcCA0285071.10
complete

MENQLSFPFVFTFLIFIIFLFKLLPKKSKSLNLPPGPSSLPLIGGFHHLFGAPPHHSLTN

LAKKFGPIFHLQLGENSNIVISSAEMAKEIMKTHDIIFADRPFIPSAFKSTYDGTDIAFS

PYGDYWRQLRKICTTELLSVNRVQSFRSIREEEVSKLISSIASSAGSAINLSRMIETLMF

SIISRAVFGKVCKGEEVFVPTIRKLTEATTGFNLVDLYPSNKLVQRMSIGLPIIKRLHSE

VDKIIQDVVDEHRARKQAGKIVAEGEEEDLVDVLLNLLEKGDLDFPLSTENIKAVIL (0)

DMFIAGSDTSSTPIEWAMSEMMKNPEVMEKAQAEVRRVFEAKGNVDEASLNELN

YLKLVIKETLRLHPPVPLLVPRECKEHCVINGYDVPEKTRIIVNAWSIGRNPEYW

TEPEKFFPERFLDSSIDYKGANFEFIPFGAGRRMCPGISFGMANVELPLAHLLYHFDWKL

QSGINPENLDMTESFSVTVKRSNALNVIPIPYIPSRVM*

 

>CYP71D JcCB0176751.20 –

BABX01064550

BABX01088561.1 end of exon 1 is 100% to BABX01064550 (join)

Exon 1 = JcCB0537261.10
66% to CYP71D326 XP_002522721.1  Ricinus communis
61% to CYP71D   JcCB0176751.10

complete

MELQILSLPFLLTFFLFIFMALKIRKISESKKLLNLPPGPLKLPIIGNIHNLVGSLPHHRLQNLAKKYG PLMHLQLGEVTTIVVTSAEIAKEVMRAHDIVFSNRPSILAANIISYNATSIVFSPYGEYW RQLRKICVLELLSAKRVQSFKSIREEEVSNIVRRISSSSDSLINLSRMLFSLTYSITSRA AFGKIRKEQEAFIPLVEEIIEVGGGFSIADLFPSIKLLNRINGMKSRVERLHQEADKILE NIINEHRASKARAKPGSKGEADDLVDVLLNIQEQGDLGFALTTNNIKAVIL (0)

DLFIAGSETSSTTVEWAMSEL

LKNTSVMKKAQAELRQVFKNKGYVDEEGVCELNYLKLIVKETLRLHPPVPLLVPRENSEL

CEINGYFIPVKSRVLINVWAIGRDPNYWKEPERFNPERFLDNSIDYKGSNFEFIPFGAGR

RICPGILFGIANVELPLANLLYHFDWKLPGEINPENLEMTEASGIAVRRKNDLNLIPITF

PSVLVA*

 

>CYP71D JcCB0123521.10 – N-term

BABX01061210.1 runs off contig at SLAD

69% to CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
62% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
68% to CYP71D   JcCB0175211.10

No ESTs to extend seq

MEYQFLSFPTFFSFLFFLFMVLKLWKRSKNNSKPNLPPGPWKLPLIGSMHHLAGALPHHR

MRDLAKKYGPIIHLQLGEFPHIVVSSPETAKQVMKTHDVIFAQRPSLLAGIIVGYNCTDL

TFAPYGEYWRQVRKISMLELLTAKRVQSFKSIREEQMSKLIREMSSGSGSPINFSKMVNS

LTYNIISTAAFGKVWSGEEMFIPAVKKVIEAAAGLSLAD

 

>CYP71D JcCB0077881.10 +  exon 2

BABX01105098

68% to CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
70% to CYP71D322   gi|255587922|ref|XP_002534441.1  Ricinus communis
71% to CYP71D   JcCA0316031.20

No ESTs to extend seq

DMFIAGSETSTTTLIWAMSEMMKNPRVIKKAQAEVRQVFNKNGNIVEEASLGEFSYLKAV

TKETLRLHPPVPVLLPRECKEDCQIDGYDIPAKSRILVNAGTIGRDPNYWTEAEKFYPER

FLDCSVDYKGTDFEFIPFGAGRRMCPGMWFGIANVEFPLAQLLYYFDWKLPNEQNPEDLD

MAEEFGAVITRKNDLRLIPTPYIPSLP*

 

>CYP71D JcCB0401681.10 +

BABX01079674 runs off contig at both ends

69% to CYP71D   JcCB0175211.10
73% to CYP71D324   gi|255563520|ref|XP_002522762.1  Ricinus communis
66% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis

No ESTs to extend seq

SDIAFAPYGDYWRQLRKICTLELLTAKRVKSFRPVREDEVSKLIRSIYSSAGSPINFSRM

FNSLTYSITSTAAFGKIWKGEEIFIPNVKNLIKASGGFSLADLYPSIKLLHLISTTRPKL

LRLHQEVDKIFQNIINEHRARKAAGKSGAESEEEDFVDVLLNFQDQGDLQFPLTDDKIKAVIV (0)

DMFVAGTETSSTTVVWAMSEML

KNPRVMEKAQAEVRHVFSKKGYVDEESVGELNYLNSVIKETLRLHPPLPLLLPRECRENC

VINGYDIPVKSKIIVNVGTIGRDPNYWIEAERFFPERFLDSSIDYKGTNFEFTPFGAGRR

MCPGILFGIVNVEFPLAQLLYYFDWKLPNGLHPENLDMIENFGSVVTRKN

 

>CYP71D JcCA0225871.10 - 

BABX01014122.1

70% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
61% to CYP71D343P   gi|255544568|ref|XP_002513345.1  Ricinus communis
87% to JcCA0149291.10

No ESTs to extend seq

DIFIAG &

SETSSTTVEWAMSEVLKNPRI

LRKAQEELRKVFDKKGKVDEEGLQEINYLKLIIKETLRMHPPGPLLIPRECRESCELNGY

KIPAKAKVIVNSWALARDPNYWTEPDTFYPERFLDSTVDFKGNNYEFIPFGAGRRICPGI

SFATPTIELPLANFLYHFDWQLPGGMKLENLDMSDGDFGTTVRRRSDLLLIPVSYNPPSLEG*

 

>CYP71D JcCA0178191.10 – exon 2

BABX01008793 Runs off end just before DMFI

70% to CYP71D326   gi|255563438|ref|XP_002522721.1  Ricinus communis
62% to CYP71D343P   gi|255544568|ref|XP_002513345.1  Ricinus communis
93% to CYP71D JcCA0267751.10

DMFIAGSA &

SSSTTIEWAMSEMVKNPSMEKAQAEVRKVFGSKGKVDEAGLHELNYLKL

VIKETLRLHPALPLLLPRQSREDCVIEGYNIATKSTVIVNAWAIARDPKYWDEAERFYPE

RFINSSIDFKGTNFEFTPFGAGRRRCPGMLFGLASVELPLAQLLYHFDWKLPGGQKPEDL

DMSDNPGGTATRRHALYLTATPNLPSAVGKI*

 

>CYP71D JcCB0307111.10 – N-term

BABX01073328.1 runs off the end at KGAI

58% to CYP71D338   gi|255544544|ref|XP_002513333.1  Ricinus communis
71% to CYP71D JcCA0290321.10

No ESTs to extend seq

MEYQMLSFPVIFSFLLFLLVLLKVSKKLCKDNSIPPPGPWQLPFLGNIFQLAGYQFHIRL

SELAKTYGPVMGIKVGQVPFLIVSSPEMAKEVLKVQDPTFVDRPVVLAAELVMYGGHDIV

YAPYGDQWRQMRKFCTLELLSTKRVQSFRSVREEEAGEFVKFLLSKEGSSVNLTHALYAL

SNSMVARSTVGHKTKNQEALLNVIDDTVSTAAGTNIADIFPSLKWLPTVKRQMSRIWKSH

CQTDEILEGILREHRAKRQTAASKNGDRAEADNLLDVLLDLQQRGDLDVPLTDINIKGAIX (0)

 

>CYP71D JcCB0222771.10 – N-term

BABX01067530  runs off the end at QEH 

No ESTs to extend seq

62% to CYP71D338 XP_002513333.1 Ricinus communis
84% to CYP71D JcCA0141621.10

MEHQVLSFPVLLSFLIFLVLLKLSKKLYKHDSNPPPGPWRLPILGNILQLAGDLPHHRLA

ELAKTYGPVMGIKLGQIPFLVVSSREAAKEVMKSLDPIFAERATILVNEIVNYNRNGMVF

GSYGDHWRQMRKFCTLELLSVKRVRSFQSVREEEMADFVKFLRSKEGSSVNLTHTIFSFT

NSIIARNAVGHKSKNQETLLRCIDGIIITGGVNIADVFPSLKWLPSVKREKSRIMKLHRE

TDEILEDILQEH

 

>CYP71D JcCB0200201.10 +  pseudogene

BABX01066138.1

82% to JcCA0285071.10
85% to CYP71D   JcCB0176751.10
70% to CYP71BE1   Vitis vinifera
72% to CYP71D322 XP_002534441.1  Ricinus communis

DMFIAGSDTSSTPIEWAMP*MMKNPEVM*KAQAEVRQVFRTKGNVD

EACLNELNYLKLVIKETLRPHPPVPLLVPRECREHCTINGYDIPERSRVIVNAWAIGRNP

 

>CYP71D JcCB0835781.10 -  exon 2

80% to CYP71D   JcCA0290321.10
67% to CYP71D338 XP_002513333.1  Ricinus communis

No ESTs to extend seq

EMFGAGSDTSTKTLEWAMSELMRNPKMMKKVQQELRSFFGENGKVEEAK &

LQELKWLKLIIKETLRLHPPIAVIPRLCRERTKVCGYDVYPNTRVFVNVWAMGRDPKIW

NEAEKFNPERFIDSSIDYRGNNFELIPFGAGKRICPGITLAIVHVETVLANLLYHFDWK

FPEGVTAENFDMNETFAGIIRRKVDLELIPVAFRP*

 

>CYP71D JcCB0472231.10 –

BABX01084551

65% to CYP71D329 XM_002522857 Ricinus communis, XP_002522903.1
68% to CYP71D   JcCA0077481.10

No ESTs to extend seq

DLFTAGTDTSSTTVEWA 

MSEMMKNPRILKKAQEEVREAFKGKKIICEEDVKQLKYLPLVIKETLRLHPPAPLLLPRE

SREACEIDGYEIPMRSKVIVNAYAIGRDPEAWPEPDNFKPERFINSSVDFKGMNFEFIPF

GAGRRICPGIAFGLANIELPLARILYHFDWKLPEGITSENLDMTEAFGATVGRKNQLYLI

PIPYTSKAESSHHSEAMKLVN*

 

>CYP71D JcCB0733471.10 -  

BABX01101510 runs of contig at both ends

BABX01071742.1 = C-term

62% to CYP71D   JcCA0019181.10
67% to CYP71D338 XP_002513333.1  Ricinus communis
ESTs GW614558, GO246714.1
complete
C-term end is not clear

MNQSVDQFASTTTSLTKIFNKIGKPIQSSPFLVSVLLLMFMASKIQNQQEEDDNSINLPP

GPWRLPFIGNIHQLAGPGLPHHRLTDLAKTYGPVMGVHLGEVYAVVVSSAETSKEVLRTQ

DTNFAERPLVNAAKMVLYNRNDMF &

FWVVGDQWRQMRKICTLELLSVKRVQSFKSVREEEMSSFIKFLSSKSGSPVNLTHHLFVL

TNYIIARTSIGKKCKNQEALLRIIDDVVEAGAGFSVTDVFPSFEALHVISGDKHKFDKLH

RETDKILEDIISEHKADRAVSSKKSDGEVENLLDVLLDLQENGNLQFPLTNDAIKGAIL (0)

DTFGAGSDTSSKTAEWTLSELIRNPEAMRKAQAEIRRVFDETGYVDEDKFEELK

YLKLVVKETLRLHPAVPLIPRECRGKTKINGYDIFPKTKVLVNVWAISRDPAIWPEPEKFN
PERFIDNPIDYKSINCELTPFGAGKRICPGMTLGITNLELFLANLLYHFDWKLPDGKMPED
LDMSESFGGAIKRKTDLKLIPVLARP

 

>CYP71D JcSR432238r.10 - 

BABX01120440.1 runs off the end at IGE

BABX01020522 runs off the end at EKFN

88% to CYP71D   JcCB0176751.10 
73% to CYP71D335 gi|255547540|ref|XP_002514827.1  Ricinus communis

No ESTs to extend seq

IGENPEYWREPEKFNPERFLDNSIDYKGAHFEFIPFGAGRRMCPGISFGMANVELPLAHL

LYHFDWKLPSGVNPENLDMTESLSVTVKRSNALYVIPIPCCHSRVM*

 

>CYP71D JcCB0741061.10 +

BABX01102121.1 runs off the end at SLT

60% to CYP71D341   gi|255544556|ref|XP_002513339.1  Ricinus communis
66% to CYP71D   JcCB0303031.20 
EST GW879869.1

MEYQILSSPTLIALLVFVATVVIKLWKRPTIANNNPPPGPWKLPLIG

NLHNLFGRDQPHHRLRDLAGKYGAV

MGFQLGQVPTVVISSAEIAKQVLKTHEFQFIDRPSLLAADIVLYNRSDIIFAPYGDYWRQ

IKKIAILELLSSKRVQSFKSVREEEVSSFFKFLYSKAGSPVNLSRTLLSLT

NGIIAKTSIGKKCKRQEEIIAVITDAIKATGGFSVADVFPS

 

>CYP71D JcCD0082551.10 + pseudogene

BABX01143592 runs off the end at DMNE

59% to CYP71D   JcCB0542951.10
61% to CYP71D341 XP_002513339.1  Ricinus communis

KIVEPDNLLDVLL &

LQRHGNLDIPLTNDSIKATIL (0)

DMITAGKDTSSAILEWAMAETLHNPRIMKKAQEEVRQAFSKKGNNIDETDLKELKFLKCI

IKETLRLHPPTPFVPR*CKARYEIDGYCIEPK

SEILVNLWAIGRDPNYFPEPEK

FYPERFIDSCVNYNGIDFELLPFGAGKRMCPGMSLGMANVELPLAHLLNHFDWKPSNDGI

KLEDIDMNE

 

>CYP71D JcCA0132211.10 + pseudogene

BABX01003657

78%  to CYP71D   JcCA0141621.10

CYP71D338 XP_002513333.1  Ricinus communis

SYRDQWRQMRKFCTLELLSAKRVRSFQSVREEVADFVKFLRSKEGSSVNL &

IKTLFSLTNSIIARNAVGHTSKNQEALLKVIDGIIESGTSVSIADNFPSLKWLPSVKRE

RSRSCKLHCETDEILEDILQEHKVNR*AASSENVDQSQLANNLLDVLLDLQQSGNLEVPV

TDVSIKAAII (0)

DV &
FVGGTDTSSKTTE*AMAELMRKPEVMKK &

AQEELRSFFGEKGYIEEVELQGLKWLK &

LIIKETMRLHPALLLLPRVCKEKTKVSGYDVYPDTRVLVNVCALGRDPKLWSEPERFNP &

ERFIDSSIDYKGNDF*YLPFGAGKRACTGSTLGMANVESFLANLLIHFDWKFPKGVNEEN

LDMNVAFGGVMKRKVDLELISIPYHP*

 

>CYP71D JcCB0285551.10 –

BABX01071742.1  runs off the end at CEL

68% to CYP71D   JcCA0019181.10
75% to CYP71D342 XP_002513342.1  Ricinus communis
EST GO246714.1

GYVDEDKFEELKYLKLVVKETLRLHPAVPLIPRECRGKTK INGYDIFPKTKVLVNVWAISRDPAIWPEPEKFNPERFIDNPIDYKSIN

CELTPFGAGKRICPGMTLGITNLELFLANLLYHFDWKLPDGKMPEDLDMSESFGGAIKRK

TDLKLIPVLARPLTPRNANSGNTFTTTDADSPASMCPHLKAL*

 

>CYP71D JcSR377678f.10 –

BABX01120300 runs off the end at LHM

47% to CYP71BE1 Vitis vinifera
53% to CYP71D JcCB0303031.20 N-term
53% to CYP71D341 XP_002513339.1  Ricinus communis 
no ESTs to extend the seq

MKQEFPYISVFSTFIFFIFILLKIFRNTKSKTSSNLPPPGPWKLPLIGNIHQLHGSLPHR

CLRDLAKTYGPVMRLQFGQVSAVVISSAELAKQVLKTDEENFLDRPRAEVTNILFYNRQG

IAFAPHGDHWKQMRDICISSMLSTRRVRSFRSIRQDEVSKFIRSIYLKAGTPINLSKMIF

SLTNKIIARTSVGEKGIREEDLKYITKEFNEAVGGFSVVDILPSLKFLHM 

 

CYP71E

 

>CYP71E JcCA0265271.10 –

54% to CYP71BC1 Vitis vinifera
complete 
47% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis
70% to CYP71E7 AY217351.1 Manihot esculenta cytochrome P450 protein CYP71E
MSISNLISLNQQWLPILAVFLLPIFTLFLFKSKKRTEGPKLPPGPRRLPIIGNFHQLGDR 

PYYDFWKMSQKHGPVMRVQLGRSPGVVISGAEASREAMKDHDLETCSRPSSVGPRRLSYN

FLDVAFSPYSDYWREMRKLFIFELLSMKRVHMFWYAREEQIDKLNNILTNAYPNPVNLTE

LIYNVIDGIMGTVAFGRSYGQVEFQQGFVKVISEAMDILNSFHAEDFFPSAGRFIDSLTG

ALAKREQTFKNLDGYFQKILERHLDPNRPKPEHEDIVDVLVGLMRDQGASFQLTKDHLKA

ILMDIFVGGIDTGSVTTAWAFTEILKNPRIMKKVQEEIRGIVGPNKNRVEGRDVDKFKYL

ELIVRETFRIHPPLPLLIPHFCTKKCKIGGYDILPGTTIFINAYAIGREPSKWENPEEFY

PERFENSDVDYRGSYFELVPFGAGRRTCPGLAMGTTAVKYTLANLLYGFDFELPNGKKFE

DFPMEEAGGPTIHNKHDLVLIPKKHEWD

 

>CYP71E JcCA0245461.10 + pseudogene

small deletion of 8 aa at frameshift &

BABX01016306

95% to CYP71 JcCA0265271.10
finished
complete
46% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis
69% to CYP71E7 AY217351.1 Manihot esculenta cytochrome P450 protein CYP71E

MSISNLISLNQQWLPILAVFLLPIFTLFLFKSKKRTEGPKLPPGPRRLPIIGNFHQLGDR

PYYDFWKMSQKYGPVMRVQLGRSPGVVISGAEASREVIKDHDLETCSRPSSVGPRRLSYN

FLDVAFRPYSDYWKEMRKLFIFQLLSMKRVHMFWYAREEQIDKLNNILTNAYPNPVNLTE

LIFNVIDGIMGTVAFGRSYGQLEFQQG &
MDILNNFHAEDFFPSAGRFIDSLTGALAKREQTFKNLDGYFQKILERHLDPNRPKPEHEDIVDVLVGLM 
RDQGASFQLTKDHLKAILM (0)

DIFVGGIDTSSVTTAWAFTEILKNPRIMKKVQEEIRGIVGPNKNRVEGRDVDKFEYLELIVRETF

RMHPPLPLLIPHFSTKKCKIGGYDILPGTTIFINAYAIGREPSKWENPEEFYPERFENSD

VDYRGSYFELVPFGAGRRTCPGLAMGTTAVKYTLANLLYGFDFELPNGKKFEDFPMEEAG

GPTVHNKHDLVLIPKKHEWD

 

>CYP71E JcCA0282051.10 - 

54% to CYP71BC1   Vitis vinifera

complete

EST GW877052.1
47% to CYP71B64 gi|255564478|ref|XP_002523235.1  Ricinus communis
70% to CYP71E7  Manihot esculenta  AY217351

MSISNLISLNQQWLPILAVFLLPIFTLFLFKSKKRTEGPKLPPGPTRLPIIGNLHQLGDR

PYYDMWKLSQKYGPVMRVQLGRSPGVVISGAEASREAMKDHDLDTCSRPLSVGPGRLSYN

FLDVAFSPYSDYWREMRKLFIFELLSMKRVHMFWYAREEQIDKLNNILTNAYPNPVNLTE

LIFNMIDGIMGTVAFGRSYGQVEFQQGFVKVISEAMDMLNSFHAEDFFPSAGRFIDSLTG

ALAKREQTFRNLDAYFQKILERHLDPNRPKPEHEDIVDVLVGLMRDQGASFQLTKDHLKS

ILMDIFVGGIDTSSVTTAWAFTEILKNPRIIKKVQEEIRGIVGPNKNRVEGRDLDKFKYL

ELIVRETFRIHPPVPLLIPHFCTKECKIGGYDILPGTTVFINTYALGREPSKWENPEEFY

PERFENSDVDYRGSYFELVPFGAGRRICPGLAMGTTAVKYTLANLLYGFDFELPNGKKFE

DFPMEEAGGLTIHNKHDLILIPKKHKWD

 

>CYP71E JcCB0047811.10 + 

54% TO CYP71BC1   Vitis vinifera

COMPLETE

70% to CYP71E7  Manihot esculenta  AY217351
47% to CYP71B64   gi|255564478|ref|XP_002523235.1  Ricinus communis
Ricinus does not have a CYP71E in the available genome sequence

MSISNLISLNRQWLPILAVFLLPIFTLFLFKSKKRTEGPKLPPGPRRLPIIGNFHQLGDR

PYYDFWKMSQIYGPVMRVQLGRSPGVVISGAEASREAMKDHDLETCSRPSSVGPRRLSYN

FLDVAFSPYSDYWREMRKLFIFELLSMKRVHMFWYAREEQIDKLNNILTNAYPNPVNLTE

LIYNVIDGIMGTVAFGRSYGQVEFQQGFVKVISEAMDILNSFHAEDFFPSAGRFIDSLTG

ALAKREQTFKNLDGYFQKILERHLDPNRPKPEHEDIVDVLVGLMRDQGASFQLTKDHLKA

ILMDIFVGGIDTGSVTTAWAFTEILKNPRIMKKVQEEIRGIVGPNKNRVEGRDVDKFKYL

ELIVRETFRIHPPLPLLIPHFCTKKCKIGGYDILPGTTIFINAYAIGREPSKWENPEEFY

PERFENSDVDYRGSYFELVPXGAGRRICPGLAMGTTAVKYTLANLLYGFDFELPNGKKFE

DFPMEEAGGLTIHNKHDLILIPKKHKWD

 

>CYP71E JcCB0345071.10 +

BABX01075737.1 , last exon

98% to JcCA0282051.10
97% to JcCB0047811.10
93% to JcCA0265271.10
92% to JcCA0245461.10
56% to CYP71BC1 Vitis
no ESTs
finished
78% to CYP71E7  Manihot esculenta  AY217351

 (0) NNYRGSYFELVPFGAGRRICPGLAMGTTAVKYTLANLLYGFDFELPNGKKFEDFPMEEAG

GLTIHNKHDLILIPKKHKWD*

 

>CYP71E JcCA0196161.10 + 

BABX01010779.1

98% to JcCB0247451.10
96% to JcCA0282051.10
93% to JcCA0265271.10
93% to JcCB0047811.10
92% to JcCA0245461.10
54% to CYP71BC1 Vitis

53% to XM_002523196 Ricinus communis

N-term, runs off the contig
72% to CYP71E7  Manihot esculenta  AY217351

MSISNLISLNQQWLPILAVFLLPIFTFFLFKSKKRTEGPKLPPGPRRLPIIGNLHQLGDR

PYYDLWKLSQKYGPVMRVQLGRSPGVVISGAEASKEAMKDHDLETCSRPLSVGPGRLSYN

FLDVAFSPYSDYWREMRKLFIFELLSMKRVHMFWYAREEQIDKLNNILTNAYPNPVNLTE

LIFNMIDGIMGTVAFGNAP

 

>CYP71E JcCB0247451.10 - 

BABX01069267.1

98% to JcCA0196161.10
59% to CYP71AS6v1 Vitis
57% to CYP71BC1 Vitis
N-term, runs off the contig
finished
69% to CYP71E7  Manihot esculenta  AY217351

MSISNLISLNQQWLPILAVFLLPIFTLFLFKSKKRTEGPKLPPGPRRLPIIGNLHQLGDR

PYYDLWKLSQKYGPVMRVQLGRSPGVVISGAEASREAMKDHDLETCSRPLSVGPGRLSYN

FLDVAFSPYSDYWR

 

CYP71AN

 

>CYP71AN JcCA0022181.10 + short, missing C-term, runs off the end

BABX01013634

47% to CYP71AN1 cottonwood 
78% to JcCA0272141.10, 73% to Ricinus communis XM_002531819 
no ESTs
finished, missing 113 aa at C-term
72% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

MDSLTFLQQWWQEPGKTYLFNPFSFCFVILLSFLLLFKPKRNNYKLNLPPSPPKLPIIGN

LYQINAFPFKSFTSLSEKYGPLMFLRLGQVPTLVVSSLELVKEITKNHDIIFADRPTLTG

VGIVLNGSPDMALGPYSDYSRDAKKLCVLQLLSQNRVQQFHFIREEEVAKIVEEIRLSTV

NIGAPINLSDMFTSLAHNILSRSAFGSLYENQNGNYKSFGELARKTTNLLSTFFFKDLFP

YLGWIDHLTGSIRNLKMVTEELNEFFDGVIKEREALMNDNEKAIDKKYLVDILLYLRNEG

LKLDLSKDNLKAILLDMFLGGSDTTAAIMEWMMAELVKNPRIMKKAQEEIRRVVGKTKSK

ITESDINXMEYLKCVMKETVRFHGSAAILRQTSAKCKLKGYDFPAKTGVIINTWA 

>CYP71AN JcCA0272141.10 - 

BABX01019283

48% to CYP71AN1   cottonwood
69% to Ricinus communis XM_002531819
missing the C-term 70 aa
finished
69% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis
MDTLTFLQLWSQEPGKISLFNPFLFCFVLVLSFLLLFKHKRNTHKLNLPPSPPTLPIIGN 

LHQMSALPHRTFRTLSEKYGPLMFLRLGQVPTLVVSSLELVKEITKNHDIIFADRPTLTG

VGIVLNGSPDMALGPYSDYSRDAKKLCVVQLLSNKRVRQFQFIREEEVEKIVEKIRTLSV

NGAPINMTDVLMSLAHNILSKSAFGSAYENQNGKNKSFDKMARKTLDLLSAFCFKDLFPF

LGWIDHLTGLVRNLKTLTRDLNEFFDRVIEEREALMNDNEKAEDQKYLVDILLYLRKEGR

ELDISKDNIKAILLDMYVGAIEATAGMMEFMMAELVKNPRIMKKAQEEVRRVVGKSKSML

TESDVNQMEYLKCVMKETVRLHGSLIIPRQTSANVKLKGYDIPAKTRVFINLWAIQMDPT

LWDRPEEFLPERFLKSSDGSNEEHKKLLLS &

FGTGRRSCP

 

>CYP71AN JcCD0055514.10 -  pseudogene

BABX01140399

68% to JcCD0065698.10
68% to Ricinus communis XM_002531819
finished
68% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

KETXR &

QTSANVKLKGYDI &

PAKTRVIINTWAIHMD &

PTLWDRP &

EFLPERFL &

xxxxxxxxxxxxxxxx

FGTGTRVCPGMSYAYAEVEYAIANLLYWFDWELPDGGRREDLDMSEVVSFAIRKKTPLWVVANSCYP*

 

>CYP71AN JcCC0123581.10 -  pseudogene

BABX01122486

53% to CYP71AN1 cottonwood, 73% to JcCD0065698.10 
76% to JcCD0055514.10
finished
71% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

YDIPEKTRVPINLWAIQRDPTLWDRPEEFLQDRFLNISDDSDQEHRKLLFSFGSGRRVCP

GLAYAYAEVEYAIANLLYWFDWELPDGGRREDLDMSEVVSFAIRKKTPLWVVANSCYP*

 

>CYP71AN23 Ricinus communis XM_002531819, XP_002531865.1

MNPITFLQERWQQQGKTALSNPFFFCSLILISILILSKPKKSSS              

KLNPPPSPPKLPIIGNLHQLSALPYRSFRTLSKKYGPLMLLHLGQVPTLVVSSMEMAK

EITKNHDVTFADRPSLTGVGIVFKGCPDMAFGPYCDHSREAKKLCVLQLLSQRRVQEF

HFIREEEVAKIVEKIRSSSINGDAINISDMFMSLAHNILSRSAFGPIYEGENGRYKSI

GELARRTMDILSAFCFKDLFPFLGWVDHLTGLIRNLKMTSTELSDFFDRVIQDRQALM

NDNEKAENKKYLVDILLQLQKEGLELDLSRDNLKAILMDMFVGGTDTTAATMEWMMAE

LMKNPRIRKKAQEETRRVVGKKSQITQADINQMRYLRCIMKEIVRFHASAMMPRQTSA

SVKLQGYDIPAKTRVLINTWAIQRDHNLWDRPEEFLPERFLNSPDDDSGNDEHKQILF

SFGTGRRVCPGMSYAYAEVEYALASLLYWFDWELPDGQSGENLDMSEVYTFVIFKKTP

LWVVAHPPSP

 

>CYP71AN JcCB0575541.10 -  possible pseudogene, or 2 seq errors

BABX01091131.1

Runs off the contig end

Two frameshifts

78% to Ricinus communis XM_002531819
no ESTs
missing N-term 16 aa
finished
78% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

1722 ISLFNPFLFCFVLVLSFLLLFKHKRNTHKLNLPPSPPTLPIIGNLHQISALPYRSFRSLSE 1540

1539 KYGPLMFLRLGQVPTLVVSSVEIAKEITK 1453 &

1451 NHDIIFADRPSLTGVGIVFNGCLDMAFGPY CDHSREAKKLCVLQLLSHRRVQQFHFIREEEVGKVVEKIRLSSVNGVPVNISDMFMSLAY NTLSRAAFGSLHENQNGNCKSFSELARKTMTVLSAFCFKDFFPYLGWIDHLTGFIRNLKM ITEELNEFFDQVIKERGAGMNDSEKAEDKKYLVDILLYLRKEGLELDLSKDNVKAILL (0)

707 DMFVGGTDTTAATMESMMAELV

KNPRIMKKAQEEVRRVVGNSKSKITESDISQMEYLKCVMKETVRCHASAMMPRQTSASVK LQGYDIPAKTRVLINTWAIQRDPALWDRPEEFLPERFLNSSDEEHKKLLFSFGTGRRVC 285 &

285 PGMSYAYAEVEYAMANLLYWFDWELPDGGRGEDLDMSEVYTFVIHRKSPLWVVAHSYSP* 106

 

>CYP71AN JcCA0124441.10 +  2 adjacent genes

BABX01002783.1

80% to JcCB0575541.10
no ESTs
77% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

  2 QTSANVKLKGYDIPAKTRVIINTWAIHMDPTLWDRPEEFLPERFLDSTDDSDGEQKNPLF 181

182 SFGTGTRVCPGMSYAYAEVEYAIANLLYWFDWELPDGARVEDLDMSEVITIVIRKKTPLWVVAHSYFP*

 

>CYP71AN JcCA0124441.10 +  2 adjacent genes

BABX01002783.1

77% to JcCB0575541.10

runs off the contig end

pseudogene

69% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

1241 MDWLTFLQQWWKEPGKTSLFNPFFFCFITFFSFLLLFNTKRDSFKLNLPPSLSRLPIIGN 1420

1421 LYQISSLPHKSFRSLSSKYG &

1480 PLSASAFGHVPTLVVSSIEIVKEITKNHDIIFANRPSFTGVGIVFNGCLDMGFGPYCDH* 1659

1660 REAKKLCVLQLLSHKRVHQFQFRREEVGKIVEKLRHSSVNGSPINMSDMIMSVAHNILS 1836

1837 RSAFGSLYEDQSGNYKP &

1890 ELARKTMNLLSAFCLKDLFPHLGWIDHLTGLIRNLKMLREELNEFFDRVIKEREALMNDN 2069

2070 EKAEDKKYLVDILLYLRKEGLELDLSIDNIKAILL (0) 2174

2269 TGFMTGIDSSAATMEFMMVELVKNPRIMKKAQEEIRRVVGNSKSKITESDISQMEYLKCGMK 2454

2455 ETVRFHGSGVVPRQTSANV 2511

 

>CYP71AN JcCB0354411.10 - 

BABX01076301.1

83% to JcCB0575541.10
75% to Ricinus communis XM_002531819
73% to JcCD0065698.10 
55% to CYP71AN1 cottonwood 
55% to CYP71AS6v1 Vitis
no ESTs
finished
75% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

TWAIHMDPTLWDRPEEFLPERFLNSSDDSKEEHKKLLFSFGTGRRVCPGMAYAYAEIEYA

IANLLYWFDWKLPNGARGEDLDMSEICTFAIRKKTPLWVVAHSYSP*

 

>CYP71AN JcCD0065698.10 +  /pseudo

BABX01141556.1

76% to JcCB0575541.10
finished
67% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

1 SKITESDISQMEYLKCVTKETIRFH 75 &

80 SAILPRQTSADVKLKGYDIPAKTRVFVNMWAIQRDPTLWDRPEEFLPERFLKTSDDSNDE 259

260 HRKLLLTFGTGRRACPGMTFAXAELEYVIANLLYWFDWEMPDEARAEDLDMSELYSFVIH 439

440 KKTPLWVVANSYCP* 484

 

>CYP71AN JcCB0534051.10 +  pseudogene

BABX01088287.1

70% to Ricinus communis XM_002531819
93% to JcCD0065698.10
76% to JcCB0575541.10
70% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

  3 LLSAFCFKDLFPYLGWIDHLTGLVRNLKMLTRDLNDFFDRVIEEREALMNDNEKAEDQEIL 185 &

185 KKYLVDILLYLRKEGRE 225 &

227 LDLSKDNLK &

    SILL (0) 253

430 DMYVGAIEATAGMMEFIMVELVRNPRIMKKAQEEVRRVVGASKSKITESDISQMEYLKCV 609

610 TKETIRFHGSAILPRQTSADVKLKGYDIPAKTRVFVNMWAIQRDPTLWDRPEEFLPERFL 789

790 KTSDDSNDEHRKLLLTFGTGRRACPGMTFAYAELEYVIANLLYWFDWKRPDGARGEDLDM 969

970 SELYSFVIHLKTPLWVVAKPYCP* 1041

 

>CYP71AN JcCB0202231.10 +

53% to Ricinus   communis XM_002531819
51% to CYP71AN1   cottonwood

complete

53% to CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

MELVALFHQWWQELDKTVPFDPLLLAPVLLLSFVFLFKLAKNRKLKLPPSPPRLPLIGNL

HQLGPLPYRSLKKLSDKYGPLMMVHFGKVPTLVVSSAEIAQEITKNHDIAFGDRPKTAAA

DDLFFGCQNLAFCPYGEYWRQVKKVCVLELLSQKRVQYFEFVRREETANLVEKLRHASLQ

GSPVDLSELLVSISNNIVSRSALGTVYNNESGHSSSGDLVRGAIDLVGSFTFQDFFPSLG

LLDVLTGFTGKVKKASKELHGFLDKVIEEHLGRSQDKADDRKDIVDILLHLEKTDMLTVD

FTRESMKAVLMDMFIGGTDTTATTMDWTMAELMKNPRIMKKAQEEVRRVVGNKSKVEESD

LDHMVYLKCVVKETLRHHVSGMIPRQTTSDVKLEGYDISANTRVLINAWGIQRDPRLWEQ

AEDFIPERFVDNPADFKGQHKEYIPFGSGRRLCPGISYALKEVEYVLANLLFLFDWKLPE

GQGPEDLDMDEVFYLVIRKKIPLMVVPSLHN

 

>CYP71AN JcCA0088631.10 -  pseudogene or two frameshifts

BABX01030913.1

64% to CYP71AN2v1 cottonwood
51% to Ricinus communis XM_002531819
50% to JcCB0202231.10
46% to CYP71AU4 Vitis
45% to CYP71AU  XM_002511252.1 Ricinus communis
finished
51% TO CYP71AN23 gi|255582142|ref|XP_002531865.1  Ricinus communis

1536 IMKGHDIVFSNRPKMTAANIFLHGCADIAF 1447 &

1445 SPYGEYWRQVRKICVLELLSLRRVQSFQFI

RDEEVADLVDKIRDACVTGDSVNISDMLASISINIISRCALGQKSEGGKFGKLSRRMMEQ

FMGFSFGDTFPFLKWMDYLTGLVFELKSTLREVDAFLDQVIEEHRNLGNHEEEDDLSAKK

DFVNILLQLEKNGTFDFQLSQEKIKAILL (0)

DMFVGGSDTTSTATEWLMAELMKNGNVMSKVQEEVRRVSGKEIKGRGQM 273 &

273 DIEQMNYLKCIIKETLRLHPPAPLLVPRET

STSVELGGYYIPPKATVLVNALAIQTDPEVW

DKPQEFWPERFENNPLDFKGQDFQFIPFGG 1

 

CYP71AP

 

>CYP71AP JcCA0223841.10 + 

75% to CYP71AP1 cottonwood

complete

73% to CYP71AP11P gi|223550411|gb|EEF51898.1  Ricinus communis

MALWLQFTNEGFIKTSLLFASIFIVLIIHLFLSKNKHNKIKKLNLPXGPKKLPIVGNLHQ

LGNMPHISLYRLSHKYGPILFLQLGEIPTVVVSSAKLAKEALKTHDLVLSSRPQLYSAKY

LFYGCTDIVFSPYGAYWRNIRKVCVLELLSAKRVQSFGYVREEEVARLVRRIGESYPQPT

NLSKMIGLYANDVLCRAALGRDFSGGGEYDRIGFEKMLNDYQDLLGGFSLGDYFPSMEFV

HRLTGMKSKLINTFRRFDRFFDSVIAEHSNSEGKTEEKKDFVDVLLDTEKNGSSSSDLPI

TMDNVKAVILDMFAAGTDTTFIILDWAMTELMMNPRVMEKAQKEVRSIVGVRTVVQERDL

HQLQYMKAVIKEVFRLHPPAPVLVPRESLEDVVIDGYDIPARTRIYINAWAIARDQEIWE

NPEIFEPERFMGSTIDYKGLDFEFIPFGAGRRICPAIAFGSAVVELALAQLLHSFDWELP

AGVKAADIDNTEAFGISVHRTVHLHVIATPHFPLSQK

 

>CYP71AP JcCB0501871.10 - 

BABX01086449.1

95% to JcCA0223841.10
91% to JcCA0063931.10
89% to JcCB0353311.10

runs off contig end

no ESTs

finished

70% to CYP71AP11P gi|223550411|gb|EEF51898.1 Ricinus communis

YDIPARTRIYINGWAIARDQEIWENPEIFEPERFMDSTIDYKGQDFEFIPFGAGRRICPG

IAFGSAVVELALAQLLHSFDWELPAGVKATDIDNTEAFGISVHRTVHLHVIAKPHFPLSQK*

 

>CYP71AP JcCA0063931.10 + 

BABX01028097.1

runs off the contig end

92% to JcCA0223841.10
91% to JcCB0501871.10
93% to JcCB0353311.10
no ESTs

finished

75% to CYP71AP11P gi|223550411|gb|EEF51898.1 Ricinus communis

FEPQRFMDSSIDYKGQDHELLPFGAGRRICPAIAFGSAVVELALAQLLHSFDWELPAGVK

AADIDNTEAFGISVHRTVHLHVIATPHFPLSQK*

 

>CYP71AP JcCB0353311.10 –

BABX01076220.1

93% to JcCA0063931.10
89% to JcCB0501871.10
86% to JcCA0223841.10

no ESTs 

finished

71% to CYP71AP11P gi|223550411|gb|EEF51898.1 Ricinus communis

FEPQRFMDSSIDYKGQDHELLPFGAGRRICPGISFGSAVVELALAQLLHSSDWELPAKVK

AADIDNTEAFGISVHRTVHLHVIAKPRFPLSQK*

 

CYP71BF like (2 sequences)

 

>CYP71BF JcCA0311741.10 + 

57% to CYP71BF1   Carica papaya

complete

70% to CYP71BF7   gi|223550412|gb|EEF51899.1  Ricinus communis

MTPNLIPPQASISKMWSSVQLLEQRYGVSSLLLPLSFTILTFSFIIFFKRSPLSNNKKTM

PPSPSRFPIIGNLHQIGLYPHRSLQSLAQIHGPIMLLHFGSTPVLVISSSEMAKEIMKTH

DIIFANRPKSRIGGKLLYQGKDIANSPYGEYWRQMKSVCVLHLLSNKRVQSFRNVREEET

TCMIKRVEKCCSDCLPVNLSEVFASLTNDVVCRVALGRKYSKERKFKELLEEFAMYLGFV

DIGDYIPWLSWINLVNGLYSKVEKVAKELDSFLDGVVEEHRDAKDGEDANNKDFVDVLLW

IQKENMAGFCMDTTSVKALILDVFAAGTDTTYTVLEWAMTELLRHPEKMKKVQNELRSEM

ANKESKVITEDDISKFDYLKAIIKETLRLHPPIPLLVPRMSTQDVRIKGFDLAAGTQVII

NSWAIGRDPALWDRPEEFWPERFLKNRIDFRGQDFELIPFGAGRRICPGIQFAMSTDELA

LANLLYKFDWALHGLERGEDLDVAECTGLTIHRKFPLLAVATPYS

 

>CYP71BF JcCD0110401.10 -  pseudogene

BABX01126258.1

47% to JcCA0204781.10 
53% to CYP726 JcCB0040361.20
56% to CYP71AS1 Citrus sinensis
62% to CYP71AU4 Vitis
64% to CYP71AU XM_002511253.1 Ricinus communis
finished
64% TO CYP71BF5 gi|223550414|gb|EEF51901.1  Ricinus communis

329 DMFVGGSDTSSTVLEWAMTELIRHPRAMKKLQSEI &

CYLKKSNITEADLDKMQYLKLVIKEALRFHV &

PFHLLAARECMQDINILGFQVPAGTMVLINAWALARDSK 6

 

>CYP71BF JcCB0695641.10 + 

BABX01099133.1

65% to CYP71AU4 Vitis
76% to XM_002511253.1 Ricinus communis
61% to CYP71AR1 strawberry
63% TO CYP71BF JcCA0311741.10
56% to CYP726 JcCA0116831.10 
55% to JcCB0021881.20
runs off the contig end
no ESTs
finished
76% TO CYP71BF5 gi|223550414|gb|EEF51901.1 Ricinus communis

DFEFIPFGAGRRGCPGMPFAMSIIELVLANLVNNFEWELPGGMKGEDLDVDESVGITTSR

KNPLLAIAIPISH*

 

>CYP71BF5 XM_002511253.1 Ricinus communis

EEF51901.1

52% to JcCA0311741.10
60% to CYP71AU4 Vitis, 56% to CYP71AU3 Vitis
56% to CYP71AU5 Vitis
100% TO CYP71BF5 gi|223550414|gb|EEF51901.1  Ricinus communis

MSSIIQSLEQQVTSIDPFYVSIFSFVFFTYFLIKWLICPASTHK                      NLPPSPPKLPILGNLHQVGGYLHRSLLSLARRYGPDLMLLHFGIKPALVVSSSDAARE                      IMKTHDLAFSNRPKFGSIGKLLYNHKDVAGGLYGDHWKQMKSVLVHHVLSNRRVQTYR                      SVREEEVACLIEKIQDLCSSSSPVNLSKMFSSFTYDVICRISFGRKYDSGDRGESGKI                      FQKLLGDLMILLGSFDLREFIPWLGWVISWVNGFDAYVDRTAKGLDKFIDGIVEEHIN                      SLERKAHSDVSKEYVKDFVQVLVELQKDTNMGANLDRESIKALILDIFAGGSDTTYTV                      LEWTMTEIIRHPRVMKELQNEVKRISDENSVITRITEADLNKMHYLKLVIKESLRLHT                      PFPLLAARETIQDVKIMGYDIAAGTMVLTNAWAMARDPKTWTKPEEFWPERFLNSCVD                      FKGHDHEFIPFGSGRRGCPGISFSMSIIELVLANLVKNFEWVLPEGTNVEDLDMTESI                      GMTTSRKNPLIAVAIPISK

 

>CYP71BF6 XM_002511252.1 Ricinus communis, EEF51900.1

62% to CYP71AU4 Vitis, 59% to CYP71AU3 Vitis

60% to CYP71AU5 Vitis,

MLDIIQFINQQISFLNPLYLTIAVLSFLFFAYFLIKWLTSSPHR                      NPPPSPPKLPILGNLHQLGKNIHRSLSSLAQRYGPDLMLLHFGSKPVVVVSSSDAARE                      IVKTHDIAFSSRPTTAVYRRLLYNNKDVAGAPYGEYWRQMRSICVLHLLSNRRVQSYR                      SVREEEVGVLIEKIKQFSSKSLPVNLSQMLSCLTNDVISRIAFGRKYSGDDGEDGIKF                      QRLLGDFMRLLGSFSVGEYIPFLGWINWINGLNKSVDRTAKELDEFIDAIVEEHMDGF                      SSEGSEEDVKDFVHVLLELQKEYGVGGSLDRESIKALILDVFAGGTDTSSTVLEWIMT                      ELIRHPRVMKELQNEVKRVAREKASTSHITEADLDKMHYTKLVIKEALRLYSPLPLLG                      PRETIQDVKVMGYHIAAGTMVLTNGWAISRDPKTWTKPEEFWPERFLNNSIDFRGHDF                      EFIPFGTGRRGCPGVSFALPVVELVLANLVKNFEWALPDGAKGEDLDLAETFGVTIHR                      KNPLLALATPTSD

 

>CYP71BF9 XM_002511247.1 Ricinus communis, EEF51895.1

62% to CYP71AU4 Vitis, 59% to CYP71AU3 Vitis

59% to CYP71AU5 Vitis,

MSNFLQLSEQHENFTLSLAFLALSFVIFLLKWSPLSSSKKFLPP                      SPPKFPIIGNLHQVGLHPHRSLRYLAQTHGPVMLLHLGSVPVLVISSAEMACEVIKTH                      DRVFADRPRSSISEKLLYHRKDIAAAPYGEYWRQMKGLSVLHLLSTKRVQSFSHVREE                      ETDYMIDRVNRFYSSSSTPLNLSEILATLTNDVVCRVALGRKHIATKGGINFKELLGE                      FVELLGFNIGTYIPWLAWINHVNGVNSRVERVAKELDNFLDGVVEEHMSSDRRDDYSK                      DFVDVLLWVQKENMAGFPIDRISIKAFILDVFSAGTDSTYTVLEWTMTELLRHPKIMK                      RLQNEVREIANSKSRITPDDLNKMQYLKAVIKETLRLHPPLPLLVPRVSTQDVKLKGF                      DIAAGTQILINAFAIGRDPASWDRPEEFWPDRFLDSSIDFKGHDFQLLPFGTGRRACP                      GIQFAISIEELALANLLYKFEWALPSGAREEDLDMTESIGLTTHRKSPLLAVATPYSH                     

QQ

 

CYP71BG

 

>CYP71BG7 JcCA0153521.30 - 

70% to CYP71BG1 Solanum tuberosum
complete
76% to CYP71BG7 gi|255547910|ref|XP_002515012.1  Ricinus communis
MDHFTPTMHPLLPLLLLFITVIGVCYYLWKTRSEMNLPSPKGRLPIIGHLHLLTDMPHHT 

FSEFSNKLGPLFYLQLGQISTVVINSAHFARLVLKTHDHAFANRPQLISAQYLSFGCSDV

TFSPYGAYWRQARKICVTELLSAKRVSSFQRVRDEEVNRLLAAVLAQSGKEVDMSKLLFN

LANDILCRVAFGRRFIEEGKEGKMVNVLTETQALFAGFCIGDFFPDWEWVNSVSGYKKRL

MKNLEDLRAVCDDIIEEHLKKKMESDDEKEDFVDVLLRVQKREDLEVPISDDNLKALVLD

MFVAGTDTSSSTLEWTLTELARHPRFLKKAQEEVRKIASGKGMVDENDLQHLHYMKALIK

ESMRLHPPVPLLVPRESMEKCILDGYEIPAKTRILINSYAIGRDPKLWENPLAYNPERFV

ENDIDIKDQDFRFLPFGGGRRGCPGYTFGLATIEIALARLLYHFDWALPDGVGADNVDLS

EVFGLATRKKSALFLVPSISKEYQLRGDEIQSRD

 

CYP73 family (only one gene and one pseudogene)

 

>CYP73A104

JcCA0130751.10 - BABX01003495  
JcCB0175681.10 + BABX01064470   

86% to CYP73A5 Arab.

Complete

95% TO CYP73A104  XP_002523952.1 cinnamate 4-hydroxylase Ricinus communis (ORTHOLOG)
63% TO CYP73A105 Ricinus communis

MDLLVLEKTLLGLFAAVIVAIVVSKLRGKRFKLPPGPLPIPVFGNWLQVGDDLNHRNLTD

LAKKFGDIFLLRMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFT

VYGEHWRKMRRIMTVPFFTNKVVQQYRYGWEDEAGKVVEDVKKNPQAATTGIVLRRRLQL

MMYNNMYRIMFDRRFESEDDPLFVKLKALNGERSRLAQSFEYNYGDFIPILRPFLRGYLK

ICKEVKERRLQLFKDFFVEERKKLSSTKSMNNEGLKCAIDHILDAQQKGEINEDNVLYIV

ENINVA (1)

AIETTLWSIEWGIAELVNHPEIQKKLRNELDTVLGPGHQITEPDTHKLPYLQAVVKETLRL

RMAIPLLVPHMNLHEAKLAGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFLEEESK

VEANGNDFRYLPFGVGRRSCPGIILALPILGITLGRLVQNFELLPPPGQSKLDTTEKGGQ

FSLHILNHSTIVAKPRSF

 

>JcCB0463761.10 +  pseudogene

BABX01083849

72% to CYP73A Jatropha

90% TO CYP73A105 XP_002534996.1 cinnamate 4-hydroxylase Ricinus communis
PROBABLE ORTHOLOG

CAIDHIIDAQ &

LKGEISEENV &

LYIVENINVAA

IETTLWSMEWAIAELVNNPSVQRKIRDEISTVLKGHPITESSIQELPYLQATVKETLRLH

TPIPLLVPHMNLEEAKLGGFTIPKESKVVVNAWWLANNPKWWKNPEEFRPERF &

FEEESGTEAVAGGKS &

DFRYLPFGMGRRSCPGII &

YLALPILGLVIAKLVSNFEMKAPKGINKIDVSEK

GGQFSLHIANHSTVVFHPIK*

 

CYP75 family (probably just two genes CYP75A and CYP75B)

 

>CYP75A53 JcCA0080681.10 -  short missing C-term

78% to CYP75A17   Glycine max
82% to CYP75A28 Vitis
no EST match to C-term
finished
77% TO CYP75A53P XP_002526066.1 flavonoid 3-hydroxylase Ricinus communis

MALNTLLLLLLLREVAISSILFFITRIFIHSLLKKPSRRLPPGPKGWPVIGALPLLGTMP

HVTLAKMSKKYGPVMYLKMGTCNMVVASTPDAARAFLKTLDLNFSNRPPNAGATHLAYNA

QDMVFADYGPRWKLLRKLSNLHMLGGKALEDWENVRAVELGQMLRAMCDASQRNEPVVVP

EMLTYAMANMIGQIILSRRVFVTKGSESNEFKDMVVELMTSAGFFNIGDFIPSIAWMDLQ

GIERGMKKLHKKFDVLLTKMIEEHMATTHLRKGKPDFLDVIMATRENSDGERLSITNIKA

LLLNLFTAGTDTSSSIIEWSLAEMLKNPNILKRAHDEMDQVIGRNRRLQESDLLKLPYLR

SICKETFRKHPSTPLNLPRVSTQACEVNGYYIPKNTRLSVNIWAIGRDPDVWENPLDFMP

ERFLS

 

>Populus trichocarpa CYP75A13 XM_002313967
83% to JcCA0080681.10

MVLLWELTMAALFFFINYLLTRCLIRKLSTRQLPPGPRGWPIIG              

AIPVLGAMPHAALAKMAKQYGPVMYLKMGTCNMVVASTPDAARAFLKTLDLNFSNRPP

NAGATHLAYNAQDMVFADYGPRWKLLRKLSNLHMLGGKALEDWAHVRVSELGHMLRAM

CEASRKGEPVVVPEMLTYAMANMIGQIILSRRVFVTKGSESNEFKDMVVELMTSAGLF

NVGDYIPSVAWMDLQGIERGMKRLHRRFDVLLTKMMEEHIATAHERKGKPDFLDVLMA

NQENLDGEKLSFTNIKALLLNLFTAGTDTSSSIIEWSLAEMLKNPRILKQAQDEMDQV

IGRNRRLEESDIPKLPYLQAICKETFRKHPSTPLNLPRIADQACEVNGYYIPKGTRLS

VNIWAIGRDPDVWDNPLDFTPERFFSEKYAKINPQGNDFELIPFGAGRRICAGTRMGI

VLVQYILGTLVHSFDWKLPKDVELNMDEVFGLALQKAVPLSAMVTPRLEPNAYLA

 

>CYP75B JcCB0126071.10 – N-term

77% to CYP75B32v1 Vitis

78% to CYP75B63 XP_002514665.1 flavonoid 3-hydroxylase Ricinus

probable N-term to JcCB0573441.10

MASFILYSILATLFVYFLFKLRSFGRRLPPGPKPWPIVGNLPHLGPKPHQSLAALARSYG

PLMHLRMGFVDVVVAASAAVATQFLKVHDANFSSRPPNCGAKYVAYNYQDLVFAPYGPRW

RMLRKISSVHLFSGKALDDFRHIRQQEVSVLTRALASAGPTPVNLGQLLNVCTANALGRV

MIGRRVFGDGTGGGDRKADEFKSMVVELMVLAGVFNIGDFVPALEWLDLQGVAAKMKKLH

ERFDAFLTEIVEEIKSNGSDECGKNKTMFSTLISLKENADDEGEKLTDTEIKALLL (0)

 

>CYP75B JcCB0573441.10 – I-helix to heme region

end of this seq is poor quality, use the EST

BABX01090958

82% to CYP75B32v1 Vitis
80% to CYP75B63 XP_002514665.1 flavonoid 3-hydroxylase Ricinus

NMFTAGTDTSSSTVEWAIAELIRHPNILAQV

RQELDMVVGQDRLVTELDLPQLTYLQAVVK

ETFRLHPSTPLSLPRIAAESCEINGYHIPKGSTLLVNVWAIARDPEEWVEPLVFRPERFL

AGGEKANVDVRGNDFELIPFGAGRRICAGMSLGLRMVQLLTS &

TLVHVFEWELADG

 

>CYP75B EST GW615118 C-term
79% to CYP75B32v1 Vitis
76% to CYP75B63 XP_002514665.1 flavonoid 3-hydroxylase Ricinus
96% to JcCB0573441.10   - 

RQELDMVVGQDRLVTELDLPQLTYLQAVVKETFRRGPSTPLSLPRIAAESCEINGYHIPK

GSTLLVNVWAIARDPEEWVEPLVFRPERFLAGGEKANVDVRGNDFELIAFGAGRRICAGM

SLGLRMVQLLTA TLVHAFEWELADG LLPEKLNMEEAYGLTLQRAVPLMVHPKPRLFLDAY

GASN*

 

>CYP75B63 Combined seqs. JcCB0126071.1 (exon1), JcCB0573441.10 (exon 2 first part)

GW615118 exon 2 second part

Complete

78% to CYP75B32v4 Vitis, 79% to Ricinus communis XM_002514619
78% TO CYP75B63 XP_002514665.1 Ricinus communis (ortholog)

MASFILYSILATLFVYFLFKLRSFGRRLPPGPKPWPIVGNLPHLGPKPHQSLAALARSYG

PLMHLRMGFVDVVVAASAAVATQFLKVHDANFSSRPPNCGAKYVAYNYQDLVFAPYGPRW

RMLRKISSVHLFSGKALDDFRHIRQQEVSVLTRALASAGPTPVNLGQLLNVCTANALGRV

MIGRRVFGDGTGGGDRKADEFKSMVVELMVLAGVFNIGDFVPALEWLDLQGVAAKMKKLH

ERFDAFLTEIVEEIKSNGSDECGKNKTMFSTLISLKENADDEGEKLTDTEIKALLL (0)

NMFTAGTDTSSSTVEWAIAELIRHPNILAQV

RQELDMVVGQDRLVTELDLPQLTYLQAVVKETFRRGPSTPLSLPRIAAESCEINGYHIPK

GSTLLVNVWAIARDPEEWVEPLVFRPERFLAGGEKANVDVRGNDFELIAFGAGRRICAGM

SLGLRMVQLLTATLVHAFEWELADGLLPEKLNMEEAYGLTLQRAVPLMVHPKPRLFLDAY

GASN*

 

>CYP75B63 Ricinus communis XM_002514619, XP_002514665.1
70% to CYP75B1 Arabidopsis thaliana
missing N-term

MAALAQVYGPLMHLRMGFVDVIVAASASVAAQFLKTHDVNFSSR              

PTNAGAKYVAYNHQDLVFAPYGPRWRLLRKISSVHLFSAKALDDFRHVREQEVGILTH

ALADACETPINLGRLLNTCTTNALGRVMIGRRVFGDGSGGGDAKADEFKSMVVELMKL

AGVFNIGDFVPAVEWLDLQGVAAKMKKLHKKFDAFLSEMVEEHKTSGSDGSEKHTDML

STLVSLKEEDDGEGGKLTDIEIKALLLNMFIAGTDTTSSTVEWAIAELIRHPKILTQV

RQELDSVVGRDRLVTEVDIAQFTYLQAVVKEVLRLHPSTPLSLPRMATESCEINGFHI

PKGSTLLVNVWAIARDPKIWTNPLEFQPERFLPTGEKSNVDVKGNDFELIPFGAGRRI

CAGMNLGLRMVNLLIATLIHAFDWELENGLKAEELNMEEAYGLTLQRLVPLIVRPRPR

LSPNVYGALST

 

CYP76 family (31 sequences, 5 are complete)

 

>CYP76F BABX01005733.1a (+) strand

JcCA0150491.30 + 

53% to CYP76B4   Medicago sativa (alfalfa)
complete
72% to CYP76F35 XP_002509652.1  Ricinus communis (partial seq)
68% to CYP76F36 XP_002509653.1  Ricinus communis

6681 MDLFICFLLILAFVHTLYFITSGRSKKRTGTMLPPGPAPLPIVGNLFDLGDRPH

NSLAELAKIHGPLMSLKLGQITTVIISSAVLAKQVLQTHDLRFSNRTVIQAIKTYDHHQV

SLPWLPVGPLWRNLRKICNCHIFTNQKLDENQGLRFKKIQELLSDVQESCSNGVAIDIGQ

AAFKVMLNSLSSTVFSLDLSDSSSDIVREFKKAVRGIMDEAGKPNLEDFFPFLRRIDPQG

IMRRMNVHVRRVLDVFDRIIDERMVLRKEPGYIPANDMLDTLLSLEQNKNEEMDRNCMKHLFV (0)

DLFSAGTDTTSSTLEWAMTELLRNPKIYLKA

QIELEQTIGKGKLPEESDVPRLPYLQAIIKETLRLHPPTPFLLPRIAGENVEMAGYVVPK

GAQVLVNAWAIGRDPSRWENPDSFMPERFLGSDIDIRGRDFELIPFGAGRRICPGLLLAI

RMLHMMLASLINSFDWKLEDGVTPENIDMEDRFGLTLEKAQHLRAIPMQV* 8254

 

>CYP76F BABX01005733.1b (+) strand (no Jc number)

pseudogene first exon does not continue with second exon

finished

62% to CYP76F36 XP_002509653.1  Ricinus communis

61% to CYP76F35 XP_002509652.1  Ricinus communis

9134 MMELLITCVLTFLLSSAFIQAVIFISKRSKKGSPCKLPPGPTPLPNLGNLLELGDK

PHKSLAKVAKIHGPLMSLKLGQVTTVVISSAALGKQFLQTFDPSFCNRTVGEAIQARGHQ

EVSLAWALVGGPWRNLRRICSPYISNQKLDSNQVLLQKKIQELVDQVEESCRLGAAVDIG

QVAFKATLNVLSTTIVSLDLSDSSSDAVRELQEVVSCIMEQAGKSNLADYYPLLRKIDPQ

GIKRRMVILFGKIFHLFDRIMDERLSLRKQQGHIP 9946 &

QNDMLHTLLSLYEDDNLDMNSIKELLL (0)

 

>CYP76F BABX01005733.1c (-) strand

pseudogene fragment between other P450 sequences

JcCA0150491.40 –

50% to CYP76Y1   Vitis vinifera
finished
54% to CYP76F36 XP_002509653.1  Ricinus communis
11259 MESYILVLFTLYLS
IAIGKRPPESLPKLAKIHGPLMTIQLGFNTTVVATSAEMAKEILQK 
NDEAFLGRPIPHAVTAEHNYELSMAWLSGRPKWKSLRKICNSHLFTPQRLDSLQGLIK 10906

 

>CYP76 JcCA0148701.10 – exon 1

BABX01005493

94% to CYP76 BABX01005733.1c

41% to CYP76 BABX01005733.1a

43% to CYP76F2 Vitis

41% to CYP76B4 Medicago sativa
no ESTs to extend seq
43% to CYP76F35 XP_002509652.1  Ricinus communis

MESCTLILITLFLLTCIHYLFTLKNNKKLPPGPRGLPIIGNLLAIGKRPHESLANLAKIH

GPLMTIQLGFNTTVVATSAEMAKEILQKNDEAFLGRPIPHAVTAEHNYELSMAWLSGGPK

WKSLRKICNSHVFTPQRLDSLQGLRNQMINGMVKSIAEASKAKEGVNIGRLVFGTTLNLL

ANSMFSGDMLDLKSINAIEELKILIGKIMELSAKPNLSDFFPFLKPLDPQGIKRQIKISY

DHMHELIEGIVGQRIKRRETSSERYGDFLDILLDQTKECGPDDFDREDVIILLA (0)

 

>CYP76F BABX01005733.1d (-) strand, exon 2

JcCA0150491.50 - 

55% to CYP76Y1 Vitis vinifera
runs off end of contig
57% to CYP76F36 XP_002509653.1  Ricinus communis

14050 DLFIGGTDTTTTTMEWVMAELLHNPNMM

AKAKQELVETIGKEKTIEEKDIIQLPYLQSILKETMRLHTTAPLLLPHKAKMDIEICGYT

IPKDTKVLVNAWALARDPKYWDDPTEFLPERFMKLGIDFRGTCFYYVPFSSGRRICPGLG

LGVRMMSLLLASLIHQFDWKLPNGMAINDMDMSDQFGTTLQKATPLTAIPLLASSS* 13436

 

>CYP76F JcCA0019761.10 -  short

52% to CYP76B4 Medicago sativa

JcCB0900091.10 + 

58% to CYP76F2 Vitis
100% to JcCA0019761.10 
73% to CYP76F36 gi|255537173|ref|XP_002509653.1  Ricinus communis

MSLKLGQITTVVISSSSLAKQVLQTFDLSFCNRPVIEAIRARGHHEVSVAWGPVGVPWRN

LRRICNSNIFANQKLDSDQDLRRKKIQELVNQVDESCRLGAAVDIGQVAFKAMLNVLSTT

IFSLDLSDSDSDIVKEFKEVVRCIMDEVGKPNLADYFPLLRKIDPQGIKGRLEIHFGLLF

DLFDRIIDERLLLRKQQSYIPSNDLLDTLLSLNEDNSEDLDMNCIKHLFLDLFAAGTDTT

SSTLEWAMTELLRNPKILSKAKAEIEQTIGKGKLIQDSDVDRLPYLQAIIKETFRLHPAV

PLLLPRRAGVDVEMCGFTVPKDAQVMVNVWAIGRDPSLWEDPDLFMPERFLGSDIDARGR

NFELIPFGAGRRICPGLPLATRMLPLMLGSLINSFGWKLEDGIIAQTMDMEDRFGVTLQK

AQPLRAIPLPLQR

 

>CYP76F JcSR522923r.10 - /pseudo needs more work

98% to JcCA0019761.10
76% to CYP76F36 gi|255537173|ref|XP_002509653.1  Ricinus communis

QCMGNWQRP*LWEDPDLFMPERFLGSDIDARGRNFELIPFGAGRRICPGLPLATRMLPLM

LGSLINSFGWKLEDGIIAQTMDMEDRFGVTLQKAQPLRAIPLPLQR

 

>CYP76G JcCB0125441.10 + 

67% to CYP76G1 Arabidopsis thaliana

missing C-term, no EST for the C-term

cannot extend

75% to CYP76G11   XP_002528643.1  Ricinus communis
71% to CYP76G12   XP_002528644.1  Ricinus communis

MDYQMVGLTALTSLLWITWAIVILITQRRRRHLKEKRPLPPGPRRWPVVGNIFQLGHRSP

HESFTELALTHGPIMTLWLGSMCTIVVSSDKMAREMFKNHDAVLAGRKIYEAMKGNHGHD

GSMITSQYGPHWRMLRRLATTEFFVTSRLDAMRTVRCRCIDELIKFIEEACGNGKNAVDV

GRFFFLMAFNLIGNLMFSKDLLDPKSETGATFFYHAGKVMEMAGKPNIADFFPFFRWLDP

QGIRRNTQFHVEKAFEIAGGFIEERMESIKKENSKEDKKKDFLDVLLEFRGDGVEEPSRF

SSRIINVIVFEMFTAGTDTTTSTLEWAMAELLHNPETLKKVQEELRSKISASKKLEEKDI

ENLPYLKAVIKETLRLHPPLPFLVPHKAMDSCKMLGYHIPKETQILVNVWAIGRDPKIWN

DPLKFRPERFFEPNMVDYRGHNFEFIPFGSGRR

 

>JcCB0960601.10 + /pseudo needs more work

48% to CYP76G1   Arabidopsis thaliana
45% to CYP76G   JcCB0125441.10

may be a false positive

51% to CYP76G11   gi|255575483|ref|XP_002528643.1  Ricinus communis

KKCSRTIARRKIYEPMKGENGNEGSLVITRY

 

>CYP76A JcCA0141361.10 –

50% to CYP76A3 Petunia 
complete
71% to CYP76A32 XP_002528653.1  Ricinus communis

MDWSYDNLLASFSLLFLATIVLLLLNQRKARHGANRRRPPGPKAWPIFGNILDLGSMPHR

TLYKLRFKYGPVLWLRLGCANTLVIQSAKAAEELFKNHDITFSDRKVAESWTAHNYYQGS

LSLGRYGSYWRFLRRIVTVELMTSKKINESTLIRQKCIDKMTEYIEEDVAAANAKGDSGE

VVVAHYVFVMAFNLIGNLVLSKDLLNARSKEGTEFSGAMDEVMEWGGKPNIADFFPFLKG

LDPQRVKKNMKVALGITINIVDKFVKERVEEKKLMKENVKRDFLDALLEYEGDGKGPDKI

STHNMLIIILEMFLAGTETTSGTTEWAMTELFRNPESMMKVKEELNQVIGQKRKVKESDI

DKLPYLQAVIKETMRLHPAIPLLIPRNTLQDTNFMGYEIPKDTQVFVNAWSIGRDPDAWE

DPLSFKPERFIGSNIDYNGQNFELLPFGSGRRICVGIPLAHRIVHFALASLLHCFDWKLD

IYSASDTLDMKERMGFTARKLKPLKAIAKKKTVSE

 

>CYP76A JcCA0272121.10 +

49% to CYP76A3 Petunia

complete

73% to CYP76A32 XP_002528653.1  Ricinus communis

MEWSYDNLLASFSLLFLATIVLLLLNQRKARHGANRQRPPGPKPWPIFGNILHLGSMPHR

TLYNLRFKYGPVLWLSLGCSNTLVIQSAKAAEELFKNHDITFSDRKVPESWTAHNYYQGS

LAFGRYGSYWQFLRRTVTVELMTSKKINDSALIRQKCIDKMIEYIEEDVATANAKGDSGE

VVVARYVFVMAFNLIGNLVLSKDLLNAQSKEGTEFFDAMDKVMEWGGKPNIADFFPFLKG

LDPQRVKKNMKVALGRTINIVDKFVKERVEEKKLMKENVKRDLLDALLEYEGDGKGPDKI

STHNMHIIILEIFFGGTETTSGTIEWAMSELFSNPESMMKVKEELNQVIGKRKVEESDID

ELPYLQAVVKETMRLHPVIPLLVPRNTLQDTNFMGYEIPKDTQVFVNAWSIGRDPDAWED

PLSFKPEKFIGSNIDYKGQNFELLPFGSGRRICVGIPLAHRIVQFALASLLHCFDWKLDS

HSAVDTLDMNERMGFTVRKLKPAKAIPKKKIF

 

>CYP76A JcCA0078231.10 + pseudo

BABX01029740.1

75% to CYP76A32 XP_002528653.1  Ricinus communis
73% TO CYP76A   JcCA0272121.10 

(0) EMFFGGTETTSGTIEWVMTELFRNPAESMKKVKEELNRVIGSNRKVEESDIDELPY

XXXXXXXXXXXXXXXXXXXX

RNTLQDTNFMGYEIPXX

TQVFVNAWSIGXXX

EAWEDPLCFKP &

ERFIGSNIDYRG &

QNFELLPFGAGRRICVGIPMAH

RVVHFAVASLLHCFDWELDSNSAAENLDVKERMGITVRKLKPVKAIPKKKMM

 

>CYP76A JcCA0072481.10 + runs off the end at HQN

BABX01029086  no EST for N-term cannot extend

69% to CYP76   JcCA0272121.10
71% TO CYP76A33 XP_002528654.1  Ricinus communis
N-TERM IS TAKEN FROM JcCA0007951.30
MEWRYSNLFTWFSFLFLATVFLLLKHRKSKSGAKLRPPGPKPWLTFGNIFNLGTTP

HQNLYKYRFKYGPILWLRLGSINTLVIQ

SAKAAEELFKNHDFSFCDRKVPECYTAHNYNQSSVSLGQYGSRWRFHRRLVTVELMTNKR

INDTTSIRQKCVNNMLQFIEEDISSAQARGESMEVEVARYVFIMSFNLIGNLVLSWDLLN

SQSKEGPEFFDALNKVARMGAKPNVADLFPFFKGSDPQRIKSNMEKELGQTLKIVEGFVR

KRIEERKLVKERENKDFLDVLLEYEGVGKEEPDKISIQNIFIMILEMFFGGTETTSSTVE

WAMAELFRSPESMRRVKEELNRVVGPKRKVEESDIHGLPYLQAVIKETMRLHPIFPLLIP

RNTLYDTNFMGYLIPKDTQVFVNVWAIARDPDVWEDPLSFKPERFIDSNIDYRGQNFELL

PFGAGRRICPGIPMAHRVLHHVVASLLHCFDWEVGSGSTPRTIDMKEGMGITVRKLIPLR

ATPKKINRE

 

>CYP76A JcCA0007951.30 + pseudo NTERM + C-TERM

BABX01029886.1

66% TO CYP76A29 XP_002528649.1  Ricinus communis
4 AA DIFFS TO CYP76A JcCA0072481.10

MEWRYSNLFTWFSFLFLATVFLLLKHRKSKSGAKLRPPGPKPWLTFGNIFNLGTTPHQNL

YKYRFKYGPILWLRLGSINTLVIQSAKAAEELFKNHDFSFCDRKVPEC*TAHNYNQSSVS

LGQYGSRWRFHRRLVTVE

 

GQNFELLPFGAGRRICPGIPMAHRVLHHVVASLLHCFDWEVGSGSTPQTIDMKEGMGNN &

VRKLIPLRATPKKINRE*

 

>CYP76A JcCB0573211.10 +  pseudogene

same as JcCA0007951.30

BABX01090940.1, BABX01029886.1

MEWRYSNLFTWFSFLFLATVFLLLKHRKSKSGAKLRPPGPKPWLIFGNIFNLGTTPHQNL

YKYRFKYGPILWLRLGSINTLVIQSAKAAEELFKNHDFS & FCDRKVPECYTAHNYNQSSVS

LGQYGSRWRFHRRLVTVE

 

GQNFELLPFGAGRRICPGIPMAHRVLHHVVASLLHCFDWEVGSGSTPQT

IDMKEGMG &

ITVRKLIPLRATPKKINRE

 

NEXT 4 SEQS ARE HIGHLY SIMILAR

 

>CYP76A JcCA0315921.10 –

52% to CYP76A3   Petunia

complete

GT981524 EST 99% to CYP76 JcCA0315921.10 (2 aa diffs)
94% TO CYP76A JcCB0089621.20
97% TO CYP76   JcCA0110571.10
66% TO CYP76A28 XP_002528647.1  Ricinus communis

MDSSPLIWSSFLLLLSLVLFLKRKNWRTENGNKNRPPGPPRWPLVGNIFDLGTLAHRILY

ELKFKYGPVLRLRIGSVDTVVIQSAKAAMELFKNHDASFCDRTVPCVFTSHNYKEGSLAL

GRFGPYWRTLRRICAIELLANRRINETVQIRRKCIDVMIRSIEDDMAAGKASGESGKVHI

PHYLFVMTFNLIGNLMLSKDLFDSKCKEGYEFFEAMDKVMVWAGTPNLADFLPFLQWLDP

QGLIRNMSRDMGRAKDIVAGFVKERIEDYKMGKKKANKDFLDVLLEFEGDGKEWEGKIPY

DKIIIIILEMFFAGSETTSSTMEWVMAELFSDPKALRKVKDELDTVVGENRKVEESDIDK

LPYLQAVIKETVRLHPVLPLLLPRNTIEDTNFMGYHISKGTQVLVNAWAIGRDPESWEDP

MNFKPERFLGSNIDFKGQNFELIPFGSGRRICVGMGLAQRVVPLGLASLLHNFEWDLDSQ

TLDMRERMAITVRKLVPLNLACRKRPTKSS

 

>CYP76A JcCB0089621.20 - 

52% to CYP76A3   Petunia

complete

66% TO CYP76A28 XP_002528647.1  Ricinus communis

MDSSSLIWSSLLLLLSLVLFLNRKNWRTDNGNKNRPPGPPGWPLVGNIFYLGTLAHRILY

EHKFKYGPVLRLRIGSMDTVVIQSAKAAMELFKNHDASFCDRTVPCVFTSHNYKEGSLAL

GRFGPYWRTLRRICAIELLANRRINETVQIRRKCIDVMIRSIEDDMAAGKASGESGKVHI

PHYLFLMSFNLIGNLMLSKDLFDSKCKEGYEFFEAMDKVVVWAGTPNLADFLPFLQWLDP

QGLKRNMSRDMGRAKDIVAGFVKERIEDYKMGKKKANKDFLDVLLEFEGDGKEWEGKIPY

EKMIIIILEMFFAGSETTSSTMEWVMAELFSNPEALRKVKDELDAVVGENRKVEESYIDK

LPYLQAVIKETVRLHPVVPLLLPRNTIEDTNFMGYHISKGTQVLINAWAIGRDPESWEDP

MNFKPERFLGSNIDYKGQNFELIPFGSGRRICVGMGLAQRVVPLGVASLLHNFEWDLHSQ

TLDMSERMGITVRKLVPFNLACRKRPAKSS

 

>CYP76A JcCB0044461.20 –

55% to CYP76A3   Petunia
98% to CYP76 JcCB0089621.20
identical to CYP76 JcCA0110571.10  
BABX01001198.1
BABX01082313.1 RUNS OFF THE END
67% TO CYP76A28 XP_002528647.1  Ricinus communis

(2) SIEDDMAAGKASGESGKVHIPHYLFVMTFNLIGNLMLSKDLFDSKCKEGYEFF EAMDKVMVWAGTPNLADFLPFLQWLDPQGLKRNMSRDMGRAKDIVAGFVKERIEDYKMGK KKANKDFLDVLLEFEGDGKEWEGKIPYEKMIIIIL (0)

EM &

FFAGSETTSSTMEWVMAELFSNPEALRKVKDELDAVVGENRKVE ESDIDKLPYLQAVIKETVRLHPVVPLLVPRSTIEDTNFMGYHISKGTQVLVNAWAIGRDPESWEDPMNF

KPERFLGSNIDYKGQNFELIPFGSGRRICVGMGLAQRVVPLGVASLLHNFEWDLHSQTLD

MSERMGITVRKLVPLNLACRKRPAKSS

 

>CYP76A JcCB0882341.10 +  pseudogene

BABX01110491.1

70% TO CYP76A28 XP_002528647.1  Ricinus communis
77% TO CYP76A  JcCB0044461.20
runs off the end at LEK

LEKIEKVEETDIDKLPYLQAVVKETLRLHPVVP &

LLVPKNTIQDTNVMGYHIPTGTQVFVNVWAIRRDPDSWEDPINLKPERF &

LGSNIDYKGQNFDLIPFGSGRRICVGMALAQRVVPFGLPSL

VHNFEWDLDKDFTPKTLDMSERIGISLRKLESLNLICKKPPMKSN*

 

>CYP76A JcCA0110571.10 -  runs off the end near SIEDD

96% to CYP76  JcCB0089621.20
67% to CYP76A28   gi|255575491|ref|XP_002528647.1  Ricinus communis

(2) SIEDDMAAGKASGESGKVHIPHYLFVMTFNLIGNLMLSKDLFDSKCKEGYEFFE

AMDKVMVWAGTPNLADFLPFLQWLDPQGLKRNMSRDMGRAKDIVAGFVKERIEDYKMGKK

KANKDFLDVLLEFEGDGKEWEGKIPYEKMIIIIL (0)

EM &

FFAGSETTSSTMEWVMAELFSNPEALRKVKDELDAVVGENRKVEESDI

DKLPYLQAVIKETVRLHPVVPLLVPRSTIEDTNFMGYHISKGTQVLVNAWAIGRDPESWE

DPMNFKPERFLGSNIDYKGQNFELIPFGSGRRICVGMGLAQRVVPLGVASLLHNFEWDLH

SQTLDMSERMXITVRKLVPLNLACRKRPAKSS

 

>CYP76A JcCB0044461.10 – C-TERM

BABX01082313.1

RUNS OFF THE END AT KPERF

69% TO CYP76A28 gi|255575491|ref|XP_002528647.1  Ricinus communis
100% TO CYP76   JcCA0110571.10

KPERFLGSNIDYKGQNFELIPFGSGRRICVGMGLAQRVVPLGVASLLHNFEWDLH

SQTLDMSERMGITVRKLVPLNLACRKRPAKSS*

 

>CYP76A JcCA0232431.10 -

BABX01014854.1

43% to CYP76A3   Petunia
97% to CYP76  JcCA0110571.10
71% to CYP76A28 XP_002528647.1  Ricinus communis
runs off the end at QWL

QWLDPQGLKR &

NMSRDMGRAKDIVAGFVKERIEDYKMGKKKANKDFLDVLLEFEGDGKEWE

GKIPYDKMIIIIL (0)

EMFFAGSETTSSTMEWVMAELFSNPEALRKVKDELDAVVGENRKVEESDIDKLPYLQAVI

KETVRLHPVIPLLIPRNTIEDTNFMGYHISEGTQVLVNAWAIGRDPESWEDPMNFKPERF

LGSNIDYKGQNFELIPFGSGRRICVGMGLAQRVVPLGVASLLHNFEWDLHSQTLDM

SERMAITVRKLIPLNLACRKRPTKSS*

 

>CYP76A JcCA0004661.10 – N-term

BABX01026107.1

88% to JcCA0246041.10
89% to CYP76   JcCB0089621.20
88% to CYP76   JcCA0315921.10
62% to CYP76A28 XP_002528647.1  Ricinus communis 
no ESTs to extend seq

MDSSSLIWSSLLLLLSLVLCLNKKKWRIDSGKKNRPPGPPGWPVVGNIFDLGTMAHRILY

ELKFKYGPVLRLRIGLVDTVVIQSAKAVMELFKNHDASFCDRTVPCVLTSHNYKEGSIAV

GRFGPYWRTIRRICAIELLANRRINETVQIRRKCIDVMIR

 

>CYP76A JcCA0246041.10 +  N-term

BABX01016372.1

91% to CYP76A JcCB0089621.20
90% to CYP76A JcCA0315921.10
88% TO JcCA0004661.10
63% to CYP76A28 XP_002528647.1  Ricinus communis
no ESTs to extend seq

MDSSSLIWSSLLLLLSLVLCLNRKNWRTDNGNKNRPPGPTGWPLVGNIFDLGIMAHRILY

ELKLKYGPVLRLRLGSVDTVVIQSAKAAMELFKNHDASFCDRTVPCVLTPHNYKDGSLAL

GRFGPYWRTLRRICAIELLANRRMNETVQIRRKCIDVMIR 

 

>CYP76A JcCB0958511.10 + 

BABX01115840.1

76% to JcCA0004661.10, 78% TO CYP76A JcCA0315921.10
67% TO CYP76A28 XP_002528647.1  Ricinus communis
RUNS OFF THE END AT VL*

VL*LRLGLVDTVVTQLAKAATELFKNHDARFCDR

MVPYVLTSHNYCEGSLAIGRFGPYWRMLRRVCAAELLANKRINETVHIRHKCIDQRIW (2)

 

>CYP76A JcCB0215111.10 + 

51% to CYP76A3   Petunia
73% TO CYP76A27 gi|255575487|ref|XP_002528645.1  Ricinus communis
RUNS OFF THE END AT WAM, NO ESTs extend seq

MWILNKQIKNIESQKTRLAMESLFPTLLVSLIILSLSTLFFLFGSRKNHRFPPGPPGWPI

LGNLLDLGTMPHRTLTKLREKYGGVIGLRLGSVNTMVILSAKPASELFKYHDLSFAERNI

TETMRSHGYNEGSLAIAPYGSYWRVLRRLVTVDMLVNKRINETASIRRKCVDDMLRWIKE

ETYEANDHGIHLARFVFLMTFNSLGNLMFSKDLVDPESKEGSEFFTAMMGLMETSGYANV

ADYFPWLRWLDPQGLKRRMDKELGKALEIASKFVKERIEEKKVVGDRRKDFLDVLLEFEG

NGKDEPAKISDRDTNIFIL (0)

EIFMAGSETTSSTIEWAM

 

>CYP76G JcCB0895001.10 -  pseudo N-TERM

BABX01111542.1

42% to CYP76G1   Arabidopsis thaliana
62% to JcCB0044461.10
50% TO CYP76G11 XP_002528643.1 Ricinus communis

RSLLLIGNIHQLSHEPHFPLAKLASTH

GYLMTLWLGPIRTIVVSSGETTKEMFNNY

XXXXXXXXXXXXXXXXXX

NEGSLVTAQCGP***FLRKLCITEFFSSSHLDDMQCLCEKPINRMLKYVEDVSESGTQAI

DLQKTFFLMAFNHIISNVM &

YRKDLEDVNIEKGXKYFYHICKVMEFSXKPNATEFFFPILKWFDPXGIKRNT*FHTRQAFEID

QSLIQEKVKSKENGWVWATKGKRKRFLRDVLLD

 

>CYP76G JcCB0044461.10 +  pseudo

BABX01082313.1

47% TO CYP76G11 XP_002528643.1  Ricinus communis

N-TERM UP TO I-HELIX REGION

FIRST 56 AA 100% TO JcCB0895001.10

RSLLLIGNIHQLSHEPHFPLAKLASTHGYLMTLWLGPIRTIVVSSGETTKEMFNNY

XXXXXXXXXXXX

MKGENGNEGSLVTAQCGP***FLRKLCITEF &

FFLSSHLDDMQCLCEKPINRMLKYIEDVSESGPKAIDLQKTFFLMAFNHIISNVMFSKGL

EDVNIEKWSKYFYHICKVMEFSGKPNATEFFPILKWFDP*GIKRNTQFHTRQGFEIGQSF

I*EKVKSKENGGYGDKEKRKDF &

REVLLDFHGNGVKPSMSSSRFMNSIILVSLFD

 

>CYP76F JcCA0005432.20 -  C-TERM (EXON 2)

BABX01026981

78% TO CYP76F36 XP_002509653.1  Ricinus communis

RUNS OFF THE END AT DLFAA

94% TO CYP76F JcCA0019761.10, NO ESTS EXTEND SEQ

DLFAAGTDTTSSTLEWAMTELLRNPKILT &

KAKAEIEQTVGKGKLIQDSDVDRLAYLQAI

IKETFRLHPAVPLLLPRRAGVDVEICGFTVPKDAQVMVNAWAIGRDPSLWEDPDLFMPER

FLGSEIDARGRNFELIPFGAGRRICPGLPLATSMLSLILGSLINSFGWKLEDEITSQTMD

MEDRFGVTLQKAQPLRAIPLPLQR*

 

>CYP76T JcCB0199131.10 +  pseudogene

BABX01066068.1

59% to CYP76T1   Populus trichocarpa
72% to JcCA0111381.10
2 aa diffs to JcCB0477351.10, deletion after DWKL

DVLDSLLMLVKESNSGLRLEDVRHMLL (0)

DFFVAGTDTTA

RTLEWAMAELLKNPEKLAKLKHEVKQVEGVIEESDIIKFPYLRATIKETFRLHPPVPFLV

PRKAENRFEPERFLDNKIDGKGRDFELIPVGAGRRICPGLTLAHRMLHLMLASLVHSFDWKL

XXXXXXXXXXXXXX

FGLSLHRTEPLLAVPCYI*

 

>CYP76T JcCB0477351.10 –

60% to CYP76T1 Populus trichocarpa, 60% w/o the AE repeat
89% to JcCB0199131.10
45% to CYP76F36 XP_002509653.1  Ricinus communis
AE repeat seems wrong
runs of the end at both ends, no ESTs extend the seq

QKHDQALSSRMVPNCLCAHNHNKNSTIWLPAIDHWRNLRKISATQLFTSQRLDARRDLRR

EKVQELLNYVDENCKINQMIDVGQAAFTTVLNLVSNTFLSIDLADYSSESSQEFNKLVID

IMDQTGSPNIADYFPILSLVDPQGVRRRMKIYLERLLQNFDGIINKRMQLSASSSSGTAE

AEAEAEAEAEAEVEANYDVLDSLLMLVKESNSGLRLEDVRHMLL (0)

DFFVAGTDTTARTLEWAMAELLKNPEKLLKLKHEVKQVEGVIEESDIIKFPYLQATIKETFRLHP

 

>CYP76T JcCA0111381.10 +   C-term

65% to CYP76T1   Populus trichocarpus

BABX01001288

Runs off the end at VLV, no EST to extend seq
58% to CYP76F36   gi|255537173|ref|XP_002509653.1  Ricinus communis
59% to CYP80C5 EEF51432.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis

VLVNVWAMGRDESISA &

NPDRFEPERFLDNKIDVKGRDFELIPFGAGRRICPGLPLGHRML

HLMLASLVHSFDWKLAHDMKPNQLDMNETFGLSLHRTEPLLVVPLH*

 

CYP77 family (3 genes, 1 CYP77A and 2 adjacent CYP77B)

 

>CYP77A23 JcCB0013411.10 –

BABX01061894 

70% to CYP77A4   Arabidopsis thaliana

frameshift in internal seq

83% to CYP77A23 gi|255580098|ref|XP_002530881.1  Ricinus communis (ortholog)

complete

MAVISSTFSLSSYNSLFFTILAFFISCFAFLFYCKSKSKNLNLPPGPTGWPIVGNLFQVA

RSGKPFFQYIEELLPKYGPIFTLKMGTRTLIILSDAKLVHEALIERGPLFATRPRENPTR

AIFSCNKFTVNAALYGPVWRSLRRNMVQNMLSSSRIKEFRHVRDDAMDKFIDRLRNEAES

NNGVVWVLRNARFAVFCILLAMCFGLEMDEETIIKMDQIMKSVLIVLDPRIDDFL &

PILSPFFSKQR

KRVSQVRKEQVEFMVPLIQKRRKALENPKSDPKAISFSYLDTLFGLKI

EGRKSSPSDQELVTLCSEFLNGGTDTTATAVEWGIAQLIDNPDVQNKLYNEIKEVVGDKK

VTETDVEKMEYLQAVVKELLRKHPPTFFLLTHAVTEATTLGGYDIPIDANVEIFSTAIGE

DPRIWSNPEKFNPDRFSSGKEQADITGVTGVKMTPFGVGRRICPGLGLATVHLHLMIARM

VQEFEWSAYPPDEKIDFTGKLEFTVVMKNNLRAKIKPRT

 

>CYP77B13 JcCA0308661.20 +

BABX01023360.1

71% to CYP77B1   Arabidopsis thaliana
87% to CYP77B13 XP_002518512.1  Ricinus communis (ortholog)

complete 

MDIIDLLILCSALIFLRLWWRYWSITGGGAINLPPGPPGWPLVGNLFQVILQRRPFIFIV

RDLREKYGPIFTMQMGQRTLVIVTSSELIHEALVQRGSTFASRPADSPIRLVFSVGKCAI

NSAEYGPLWRTLRRNFVTEVTSPVRIKQCSWIRQWAMENHMERIKREAFENGSVEVMSNC

RLTICSILICICFGARISEDRIKHIEAVLKEVMLMTTPKLPDFLPVLAPLFRRQMKEAKE

LRRKQMECLFPLIRNRRAFIEKGGNPNEEMASPVGAAYIDSLFELKPANRGQLGEEEFVT

LCSEVISAGTDTSATTIEWALLHLVQNQEIQEKLYKEIVECVGKDGVVKEEDVEKMRYLG

AVVKETLRRHPPSHFLLSHAATKETELGGYTIPAGVNVELYTAWVTEDPEMWEDPREFRP

ERFLKGDGVDVDLTGTRGLKMVPFGAGRRICPAWSLGTLHVNLLLARMIQAFKWLPDPHS

PPDPTEAFAFTVVMKNPLKAVILPRS

 

 

CYP77B second gene

 

>CYP77B JcCA0308661.10 –

BABX01023360.1 two CYP77B genes present

Runs off contig at PQL

75% to  JcCA0308661.20
88% to CYP77B14 XP_002518513.1  Ricinus communis
GT976824.1 spans the gap between first two contigs

MELIDLFILCFSFVFLALWWRSFLTPTKSKNLPPGPPGWPLVGNLFQVIFQRRHFIFIIR

DLRKKYGPIFTMKMGQRTLVIVTSPDLIHEALIQRGPVFASRPPDSPIRLVFSVGKCAVN

SAEYGPLWRTLRRNFVTELMSPVRIKQCSWIREWAMENHMKRLENEAFEKGCVDVMDICR

FTVCSILVCICFGAKIPEHWIKDIDSVTKEVMLITTPQL 219

 

>JcPR03EFLJT.10 + 

BABX01047264.1 runs off the end at RAK

65% to CYP77B   JcCA0308661.20
57% to CYP77B1   Arabidopsis thaliana

270 RAKELRKTQMECLVPLIRNRRAYVEKGDNPGSLMVSPVGAAYVDSLFGLEAPGRGRLGEE

ELVTLCSELFVAGIDTSTSVLQWAFLELVLHQDIQEKLYKEIVRQCGQK &

MGLITEDNVEKMTY &

LNAVVKETLRVHSP 405

THFTLSLRHHGETELGGYKIP

 

>JcCD0088070.10 –

BABX01144240.1

TELGGYKIPTNVL &

VEFYIEWMTEDPNLWKDPDVFRPERFLEGDGVDVDLTGTKGTVKML

PFGAGRRTCPGLALGVLHVNLMLARMVQAFKWVPTPKLGQPDPTETFAFTVVMKNPLKAVILPR* 538

 

>CYP77B14 Combined sequences JcCA0308661.10 plus EST plus two other contigs

71% to CYP77B JcCA0308661.20

88% to CYP77B14 XP_002518513.1  Ricinus communis (ortholog)

MELIDLFILCFSFVFLALWWRSFLTPTKSKNLPPGPPGWPLVGNLFQVIFQRRHFIFIIR

DLRKKYGPIFTMKMGQRTLVIVTSPDLIHEALIQRGPVFASRPPDSPIRLVFSVGKCAVN

SAEYGPLWRTLRRNFVTELMSPVRIKQCSWIREWAMENHMKRLENEAFEKGCVDVMDICR

FTVCSILVCICFGAKIPEHWIKDIDSVTKEVMLITTPQL

PDFLPIFTPLFRRQMK

RAKELRKTQMECLVPLIRNRRAYVEKGDNPGSLMVSPVGAAYVDSLFGLEAPGRGRLGEE

ELVTLCSELFVAGIDTSTSVLQWAFLELVLHQDIQEKLYKEIVRQCGQK &

MGLITEDNVEKMTY &

LNAVVKETLRVHSP 405

THFTLSLRHHGE

TELGGYKIPTNVL &

VEFYIEWMTEDPNLWKDPDVFRPERFLEGDGVDVDLTGTKGTVKML

PFGAGRRTCPGLALGVLHVNLMLARMVQAFKWVPTPKLGQPDPTETFAFTVVMKNPLKAVILPR* 538

 

CYP78 family (7 sequences, 6 different genes, 4 are complete)

 

>CYP78A95a JcCB0739451.10 +  N-term

BABX01101991 runs off the end at RAAE

48% to CYP78D2   Populus trichocarpa
69% to JcCB0238121.10
72% to CYP78A99 EEF43444.1  Ricinus communis
92% to CYP78A95 XP_002533334.1  Ricinus communis (Ortholog)
(28 aa overlap at end)
No ESTs to extend seq

MRTDIDSLWVFALTSKCRVFTQENLAWSFLIICLAWLAIALLHWAYPGGPAWGKHMLKKG

FPAFSKPIPGPRGLPLIGSMSLMVSLAHRRIAAAAQTCKAKRLMAFSLGDTRVIVTCNPD

VAKEILNNSVFADRPVKESAYRLMFNRAIGFAPYGVYWRTLRRIAATHLF &

CPKQIRAAE

 

>CYP78A95b JcCB0239161.10 +  C-term

BABX01068741 runs off the end at RPRD

possible C-term for JcCB0739451.10

72% to JcCB0238121.10 combined
85% to CYP78A95 XP_002533334.1  Ricinus communis (Ortholog)
No ESTs to extend seq

337 RPRDFVDVLLSLEGPERLTDADMIAVLWEMIFRGTDTVAVLIEWVLARVILHPDVQSTVH

DELDKVVGRSRTVDESDITAMVYLQAVVKEVLRLHPPGPLLSWARLAITDTTIDGYHVPA

GTTAMVNMWAIAREPELWANPLEFIPERFLAKEGEVEFSVLGSDLRLAPFGSGRRTCPGK

NLGLTAVTFWVASLLHEFEWLPSDGTNIIDLSEVLGLSCEMANPLTVKVLPRRPSFY*

 

>CYP78A96 JcCB0658201.10 -  end of exon 1

BABX01096755 runs off the end at LLRK

43% to CYP78A5   Arabidopsis thaliana
64% to CYP78   JcCA0262611.10
86% to CYP78A96 XP_002532268.1  Ricinus communis (Ortholog)
no ESTs for this seq 

LLRKIASSHLFSPRRILAHETLRQLECASMLRNIANEQTQNGRVYLRKHLQFASLNNIMG

SVFGKRYDPAHDSKELEELRDMVREGFELLGAFNWCDYLQWLSYFYDPFRINERCLKLVP

RVRKFVRGIIEEHRLGESRNVSDNGDFVDVLLSLDGEEKLQEDDMLAVLW (0)

 

>CYP78A97 JcCA0262611.10 - 

51% to CYP78D2 Populus trichocarpa
51% to CYP78A5 Arabidopsis thaliana
79% to Populus trichocarpa cytochrome P450 CYP78A20
78% to CYP78A97 XP_002527123.1  Ricinus communis (Ortholog)
complete
MDLSSASLDSSWWVFTLPATLQTHNLLNPMFLFFVLISFLVITSSTWFFSNGGLAWRNCR 

NQMGIISIPGPRGLPWFGSLLSLSHGLPHRTLSRMASALSAKQLMAFSIGSTPAVVTSDP

HIAREILTSTNFANRPIKQSAKSLMFSRAIGFAPNGAYWRLLRRISSVHLFSPKRILAHE

PWRKQACDIMLRLVQKEQSLRGVVSLRKHLQNASLNNIMGSVFGKRYDLMQFSEETKELH

ELVSEGFELLGAFNWSDYLPWLNHFYDPVHVKERCSVLVPKVNKLVKQIIEEHYDQSKKP

KELLDNSDFVDVLLSLDGDEKLQEDDMVAVLWEMIFRGTDTTALLTEWIMAELVLNPQIQ

ATLRNEVDLVVGDKSTVTDAVISKLPYLQAVVKETLRMHPPGPLLSWARLSTSDVHLSNG

MVVPKNTTTMVNMWAITHDPKIWEDPMVFKPERFMESLGGADVDVRGGDLRLAPFGAGRR

VCPGKNLGLATVNLWVAKLVHHFEWVQDMANPVDLSEVLRLSCEMKNPLCAVAIPRNK

 

>CYP78A98 JcCA0137911.10 - 

64% to CYP78A5 Arabidopsis thaliana
82% to CYP78A98 XP_002514632.1  Ricinus communis (ortholog)
complete
MSQDFVFECFSFLSRTGSSPFFSFEVVLCLFLFVSIFSFWLAPGGLAWVLYKTKFNVQSK 

TAIPGPSGLPVLGMVFAFTGSLTHRVLAKTCNLVKAKPLMAFSIGFTRFIISSHPETAKE

ILNSSAFADRPVKESAYELLFHRAMGFAPFGEYWRNLRRISSTHLFSPKRISSFGEFRTQ

IGRKMVDEIKGLVEKNGEVEIRRILHFGSLNNVMKSVFGKSYEFSDAKDVEGSELEGLVT

EGYELLGIFNWSDHFPFFGWLDLQGVKKRCRNLVAKVNVFVGKVVEEHRIMRRQDRNHEA

NENGDFVDVLLDLEEENKLNDSDMIAVLWEMIFRGTDTVAILLEWILARMVIHQEIQSKA

QSEIDQVVGLNRLVSDSDLPNLPYLRGIVKETLRMHPPGPLLSWARLAIHDTQIGHYFIP

AGTTAMVNMWSITHDEQFWSEPEKFKPERFLEEDVAIMGSDLKLAPFGSGRRVCPGKAMG

LATVELWLAQLLQSFKWVTTNNGEVDLSEKLKLSLEMKNPLVCKALSRASA

 

>CYP78A99 JcCB0238121.10 + 

BABX01068664.1 runs off the end at TTAM

Total seq is 52% to CYP78A5   Arabidopsis thaliana

JcCB0028141.30 – C-term, possible end for JcCB0238121.10 (joined)

BABX01071418 runs off the end at GPEV

57% to CYP78A5   Arabidopsis thaliana
69% to JcCB0239161.10
total seq 50% to CYP78A JcCA0137911.10
75% to Populus trichocarpa cytochrome P450 (CYP78A22)
81% to CYP78A99 EEF43444.1  Ricinus communis (Ortholog)
complete

METQLESFWVLALVSKCNSLSSHSTFFLLMFLCMAWLTMSLCYWAYPGGPAWGKHWLIKR

ESKIIPGPRGFPLLGSMNLMANLAHHKLAAAANLFKAKRLMAFSLGDSRVIVTCNPDVAK

EILNSSVFADRPLKESAYGLMFNRAIGFAPYGLYWRTLRRIAATHLFCPKQINLTETQRS

DIGSQMVSLIACQKGDFQIRSILKKASLNNMMWSVFGRRYELSSYNSETEQLRGLVEEGY

ELLGKLNWSDHLPWLACLDFQKIRFRCSQLVPKVNHFVNQIIDQHKSQLNYSQRKQDFVD

VLLSLQGPDKLSDQDMVAVLWEMIFRGTDTVAVLIEWILARMVLHPEIQSKVHHELDRVV

GRSRPLMESDIQSMIYLQAVVKEVLRLHPPGP &

LLSWARLAITDTKVDGYEVPQGTTAMxxxxxxxx

GPEVWMDPVKFWPERFMCKDTDTEFSVLGSDLRLAPFGSGRRTCPGKALGLATVSFWVGT

LLHEFEWVQSDRDPVDLTEVLRLSCELANPLSVKVYPRRRGC*

 

>CYP78D5 JcCA0133671.10 - 

76% to CYP78D2 Populus trichocarpa

GT972937

79% to CYP78D5 XP_002514920.1  Ricinus communis (Ortholog)
complete
MKTSILLQLSFLLFFLAIITTYETQSSFLLTLFFILFFSLFTFALNYWLIPGGFAWRNHY 

YHQELVGPFGWPLLGFLPQMGSLAHRKLASMANSLGATKLMAFSLGATRVVISSHPDTAK

EILSGSCFSDRPIKESARLLMFEKAIGFAPSGDYWRHLRRIAANYMFSPRRISGLEPTRQ

RLADKMIAEVRKEMDERGTVVLREILQKGSLSNVLESVFGRNCVCLEKEELLSCMVKEGY

ELITKFNLEDYFPLRFLDFYGVKRRCYKLAGQVNSVVGEIVRERKRDGSFNSGSDFLSAL

LSLPEQEQLSESDMVPVLWEMLFRGTDTVAILLEWIMARLVMHQDIQAKAQKELNACIGS

NRHVQDSDIPNLPYLQAIVKEVLRMHPPGPLLSWARLAIHDVHVGKILIPAGTTAMVNMW

AITHDPSIWKDPCEFNPDRFLNDDIAIMGSDLRLAPFGSGRRVCPGKALGLATVQLWLAR

LLHQYKWLPAKPVDLSECLRLSMEMKWPLACRVVNRWSECEAI

 

CYP79 family (24 sequences, 6 are complete)

 

>CYP79D JcCB0045301.10 + 

78% TO CYP79D1 Manihot esculenta

GW61520 only CYP79 EST

75% to CYP79D26 XP_002534163.1 Ricinus communis 
next best match 52% to CYP79D28 XP_002523441.1 Ricinus communis

COMPLETE

MAMNVSSTVFLTPATSTSTTNTIKALLVTLFISIVSTVIKLQRRAALKKTSKILPLPPGP

KPWPFVGNIPEMIRYRPTFQWVDQLMKEMKTDICLIRFGRTNVIPVNCPIIARELLKKND

AIFSNRPMILSAKEMSGGYQTTIVVPNNDQWKKMRKILTSEIISPAKHKWLHDKRTEEAD

NLVFYIHNQYKANNNVNLRLAARHYCGNVIRKMMFSKRYFGEATPDGAPGRVEIEHVDSV

FAVLKYLYSFCISDFMPFLLGLDLDGQEKFVLEANKTLRAFHNPIIDERIELWRSGQRKE

MKDLLDVFITLQDSDGKPLLTPDEIKSQVAEVMIATIDNPSNAVEWAMGELLNQPEILKK

ATEEIDRVVGKDRLVQESDIPDLNYVKACAREAFRLHPVAYFNPPHVAAQDTEICGYLIP

EGSWVMLSRYGLGRNPKTWPNPLKYDPERHLNEGEVVLTEHGLRFISFSTGRRGCIAALL

GTCMTTMLLARMLQCFTWSLPDNVEKIDLSEGVDELFPANPVVAFPKPRLALHLYPTSP*

 

>CYP79D JcCA0257981.10 +   

BABX01017703

98% to CYP79D JcCB0045301.10
74% to CYP79D26 XP_002534163.1 Ricinus communis 

MAMNVSSTDFLTPAAAATSTSTTNTIKALLVTLFISIVSTVIKLQRRAALKKTTKILPLPPGPKPWPF

VGNIPEMIRYRPTFRWVDQLMKEMNTDICLIRFGRTNVIPVNCPIIARELLKKNDAIFSN

RPMILSAKEMSGGYQTTIVVPNNDQWKKMRK &

ILTSEIISPAKHKWLHDKRTEEADNLVFYIHNQYKANNNVNLRLAARHYCGNVIR

KMMFSKRYFGEATPDGAPGRVEIEHVDSVFAVLKYLYSFCISDFMPFLLGLDLDGQEKFV

LEANKTLRAFHNPIIDERIELWRSGQRKEMKDLLDVFITLQDSDGKPLLTPDEIKSQVA

EVMIATIDNPSNAVEWAIGELLNQPEILK

KATEEIDRVVGKDRLVQESDIPDLNYVKACAREAFRLHPVAYFNPPHVAAQDTEICGYLI

PEGSWVMLSRYGLGRNPKTWPNPLKYDPERHLNEGEVVLTEHGLRFISFSTGRRGCIAAL

LGTCMTTMLLARMLQCFTWSLPDNVEKIDLSEGVDELFPANPVVAFPKPRLAPHLYPTSP*

 

>CYP79D JcCB0007881.20 +

77% to CYP79D1 Manihot esculenta
74% to CYP79D26 gi|255587166|ref|XP_002534163.1  Ricinus communis
97% to CYP79D JcCA0257981.10
97% to CYP79D JcCB0045301.10
complete 

MATNVSSTAFLTPAAAATSTSTTNTIKALLVTLFISIVSTVIKLQRRAALKKTSKILPLP

PGPKPWPFVGNIPEMIRYRPTFRWVDPLMKEMNTDICLIRFWKTNVISVNCPIIARELLK

KNDAIFSNRPMILSAKEMSGGYQTTIVVPNNDQWKKMRKILTSEIISPAKHKWLHDKRTE

EADNLVFYIHNQYKANNNVNLRLAARHYCGNVIRKMMFSKRYFGEATPDGAPGRVEIEHV

DSVFAVLKYLYSFCISDFMPFLLGLDLDGQEKFVLEANKTLRAFHNPIIDERIELWRSGQ

RKEMKDLLDVFITLQDSDGKPLLTPDEIKSQVAEVMIATIDNPSNAVEWAMGEMLNQPEI

LKKATEEIDRVVGKDRLVQESDIPDLNYVKACAREAFRLHPVAYFNPPHVAAQDAEICGY

LIPEGSWVMLSRYGLGRNTKTWPNPLKYDPERHLNEGEVVLTEHGLRFISFSTGRRGCIA

ALLGTCMTTMLLARMLQCFTWSLPDNVEKIDLSEGVDELFPANPVVAFPKPRLAPHLYPT

SP*

 

>CYP79D JcCB0045301.20 + C-term exons

BABX01082982

98% to JcCB0089691.10, 98% to CYP79D JcCB0007881.20
80% to CYP79D26 XP_002534163.1  Ricinus communis

EVMIATIDNPSNAVEWAMGELLNQPEILKKATEEIDRVVGKDRLVQESDIPDLNYVKACA

REAFRLHPVAYFNPPHVAAQDTEICGYLIPEGSWVMLSRYGLGRNPKTWPNPLKYDPERH

LNEGEVVLTEHGLRFISFSTGRRGCIAALLGTCMTTMLLARMLQCFTWSLPDNVEKID

LSEGVDELFPANPVVAFPKPRLALHLYPTSP*

 

>CYP79D JcCB0643401.10 -  

BABX01095860 runs of the end at SEII

98% to CYP79D JcCB0007881.20 (5 aa diffs)
77% to CYP79D26 XP_002534163.1  Ricinus communis

SEIISPAKHKWLHDKRTEEADNLVFYIHNQYKANNNVNLRLAARHYCGNVIRKMMFSKRY

FGEATPDGAPGRVEIEHVDSVFAVLKYLYSFCISDFMPFLLGLDLDGQEKFVLEANKTLR

AFHNPIIDERIELWRSGQRKEMKDLLDVFITLQDSDGKPLLTPDEIKSQVAEVMIATIDN

PSNAVEWAMGEMLNQPEILKKATEEIDRVVGKDRLVQESDIPDLNYVKACAREAFRLHPV

AYFNPPHVAAQDAEICGYLIPEGSWVMLSRYGLGRNPKTWPNPLKYDPERHLNEGEVVLT

EHDLRFISFSTGRRGCVAALLGTCMTTMLLARLLQCFTWSLPDYVEKIDLSEGVDELFPA

NPVVAFPKPRLAPHLYPTSP*

 

>CYP79D JcCB0089691.10 + 

BABX01111691 runs off the end at VFA

98% to CYP79D   JcCB0007881.20 (6 aa diffs)
77% to CYP79D26 XP_002534163.1  Ricinus communis

VFAVLKYLYAFCISDFMPFLLGLDLDGQEKFVLEANKTLRAFHNPLIDERIELWRSGQRK

EMKDLLDVFITLQDSDGKPLLTPDEIKSQVA (0)

EVMIATIDNPSNAVEWAIGEL

LNQPEILKKATEEIDRVVGKDRLVQESDIPDLNYVKACAREAFRLHPVAYFNPPHVAAQD

AEICGYLIPEGSWVMLSRYGLGRNPKTWPNPLKYDPERHLNEGEVVLTEHGLRFISFSTG

RRGCIAALLGTCMTTMLLARMLQCFTWNLPDNVEKIDLSEGVDELFPANPVVAFPKPRLA

PHLYPTSP*

>CYP79D JcCB0386341.10 – runs off the end at GELL

BABX01078712

93% to CYP79D JcCB0007881.20
70% to CYP79D26 XP_002534163.1  Ricinus communis

MATNVSSTAFLTSAAAATSTSTTNTIKALLVTLFISIVSTVIKFQRRAALKKTTKILPLP

PGPKPWPFVGNIPEMIRYRPTFRWVDQLMKEMNTDICLFRFGRTNVIPVNCPIIARELLK

KNDAIFSNRPMILSAKEISGGYQTTIVVPNNDQWKKMRKILTSEIISPARHKWLHDKRAE

EADNLVFYIHNQYKANNNVNLRLVARHYCGNVIRKMVFSKRYFGEATPNGGPGCVEIEHV

DSVFAVLKYIYAFCISDFMPFLLGLDLDGQEKFVLEANKTLRAFHNPLIDERIELWRSGQ

RKEMQDLLDVFITLQDSDGKPFLTSDEIKNQVA (0)

EVMIATIDNPS &

NAVEWTM &

GELL

 

>CYP79D JcCD0109805.10 +

BABX01126166.1

67% to CYP79D26 XP_002534163.1  Ricinus communis 
94% TO CYP79D   JcCB0386341.10
runs off the end at DSV, covers end of exon 1

DSVFAVLKYIYAFCISDFMPFLLGLDLVGQEKFVLEANKTLRGFHNPLIDERIALWRSGQ

RKEI &

QDLL (0)

 

>CYP79D JcCA0009561.10 –

BABX01031693 one frameshift at &, no exons

54% to CYP79D1 Manihot esculenta
85% to JcCA0077011.10
71% to CYP79D31   = old CYP79A13 XP_002523435.1  Ricinus communis
71% to CYP79D29   XP_002523438.1  Ricinus communis
71% to CYP79D28   XP_002523441.1  Ricinus communis

MAFPQLIFSFSFITILMGTITSLLIYSIKSHKPPSKDEKSILRQQLPPGPKPWPIIGCLL

AKYRNEPAFRWIHNLMKQMNTEIACIRLGNVHVIPVTCPKISCEILKAQDANFATRPISM

STRFTSKGYLATALAPFGDQWKKMRGVLVTQLLSPAKQRRFYGTRVEEANHLVRYAYNQC

NEGGLVDVRVAAQHYCGNVIRKMMFNKRFFGKGRKNGGPGFEEEEHIDAIFRVLHHLYAF

CLSDYLPCLVGFDLDGHEKIIKESNGIIGKYHDPIIEDRVQQWKDGTKKEEEDLLDVLIT

LKDDNGNPLLSTDEIKAQITEIMLETVDNPSNAVEWAIAEMINQPKILEKAVEELDRVVG

KERLVQESDLSQLNYITACAREAFRLHPVVPFNVPHVSMTDTTVANFFIPKGSHVLLSRV

GLGRNPKIWD &

KPHKFKPEHHIKNNGSQVTLIDPYLNFISFSTGRRGCPGVTMGTSMTLMLFARLLQGFTW

NVPPNQTSINLTESKSSLGLAKPLVALVKPRLPAHVYDQIMHDQ*

 

>CYP79D JcCB0697441.10 -  C-term exons

BABX01099288

95% to JcCB0931751.10, 89% to CYP79 JcCA0009561.10
76% to CYP79D27P   gi|255564902|ref|XP_002523444.1  Ricinus communis
74% to CYP79D31   = old CYP79A13 gi|255564884|ref|XP_002523435.1  Ricinus communis
INQPKIFEKAAKELDKVVGKERLVQESDLSQLNYI 

TACAREAFRLHPVSPFNIPHVSITDTTVANYFIPKGSHVLLSRVGLGRNPKIWDEPHKFK

PERHIKNDGSQVTLIEPYLNFISFSTGRRGCPAVTMGTSMTLMLFARLLHGFTWNVPSNQ

TSIELKESESSLTLAKPLVALVKPRLPAYVYDQIMHDQ*

 

>CYP79D JcCB0931751.10 + C-term exons

BABX01113920 runs off the end at VDN
95% to JcCB0697441.10, 88% to CYP79   JcCA0009561.10
75% to CYP79D31   = old CYP79A13 gi|255564884|ref|XP_002523435.1  Ricinus communis

xxxxxxVDNPSNAVEWAIAEMINQPKIFEKAAEELDKVVGKERLVQESDLSQLNYITACAREAFRL

HPVSPFNIPHVSITDTTVANYFIXKGSHVLXSRVGLGRNXKIWDEPHKFKPERHIKNDGS

QVKLVEPYLNFISFSTGRRGCPXVTMGTSMTLMLFARLLHGFTWNVPPNQTSIDLKESES

SLTLAKPLVALVKPRLPAYVYDQIMHDQ*

 

>CYP79D JcCA0180311.10 - C-term exons

BABX01009009
91% to JcCB0697441.10, 83% to CYP79 JcCA0009561.10
74% to CYP79D31   = old CYP79A13 gi|255564884|ref|XP_002523435.1  Ricinus communis

EIMSATVDNPSNAVEWAIAEMINQPKIFEKAAEELDKVVGKERLVQESDLSQLNYI

TACAREAFRLHPVSPFNIPHVSITDTTVANYFIPKDSHVXLSRVGLGRNPKVWDEPHKFK

PERHIKNDGSGVTLTEPDLRFISFSMGRRGCARVTMGTSITLMLFARLLQGFTWNVPRNE

TSIDLKESESDLTLAKPLVLLAKPRLPAYVYDQIMHDN*

 

>CYP79D JcCA0276031.10 - C-term exons

BABX01019715
90% to JcCA0180311.10, 86% to CYP79   JcCA0009561.10
77% to CYP79D27P   gi|255564902|ref|XP_002523444.1  Ricinus communis

EIMLATVDNPSNAVEWAIAEMINQPKIFEKAVEELDKVVGKERLVQESDLSQLN

YITACAREAFRLHPVGPFNLPHVSIADATVANYFIPKGSHVLLSRMGLGRNPKVWNEPYK

FKPERHIKNDGSGVTLTEPDLRFISFSTGRRGCAGVTMGTSMTLMLFARLLQGFTWNVPP

NQASIDLKESDSSLTLAKPLVALVKPRLPAYVYDQIMHDQ*

 

>CYP79D JcCA0267971.20 +  C-term

BABX01018813.1

53% to CYP79B2   Arabidopsis thaliana 
79% to CYP79   JcCA0276031.10
67% to CYP79D30 XP_002523436.1  Ricinus communis
2 AA DIFFS TO JcCB0172531.10   +  C-term

FSKGRRGC &

TMGTSMTLMLFARLLQGFTWKVPPNQTSIDFKESESNLTLAKPLIALAKLRLPTH

VYCEIIHN*

 

>CYP79D JcCB0172531.10 +  C-term

BABX01064250.1

51% to CYP79B2   Arabidopsis thaliana
96% to JcCA0267971.20
78% TO CYP79 JcCB0931751.10
69% TO CYP79D27P XP_002523444.1  Ricinus communis

FSKGRRGC &

TMGTSMTLMLFARLLQGFTWKVPPNQTSIDFKESESNLTLAKPLIALA

KLRLPTYVYGEIIHN*

 

>CYP79D JcSR112794r.10 + C-term exons

/pseudo runs off the end AT VEW

BABX01119666

94% TO CYP79 JcCA0009561.10
77% to CYP79D27P   gi|255564902|ref|XP_002523444.1  Ricinus communis

VEWAIAEMINQPKILEKTVEELDRVVGKERLVQESDLSQLNY

LTACAREAFRLHPVVPFNVPHVSMTDTTVANFFIPKGSHVLLSRVGLGWNPKIWDEPHKF

KPERHIKNDGSQLTLIDPYLNFISFSTGRRGCPGVTMGTSMALMLFARLLQG

 

>CYP79D JcCA0077011.10 + exon 1

85% to JcCA0009561.10

64% to CYP79D28   gi|255564896|ref|XP_002523441.1  Ricinus communis
68% to CYP79D29   gi|255564890|ref|XP_002523438.1  Ricinus communis

MSFSHQIAFPQLIFSFSFITIMMGTITSLLIYSIKSHKPPSKNEKSILRQQLPPGPKPWP

IIGCLLAMYKNEPAFRWIHNLMKQMNTEIACIRLGNVHVIPVTCPKISCEILKAQDSNFA

TRPITMSTRVTSKGYLTTVLAPYGDQWKKMKRILVTQMLSPAKQLWFSGKRAEGADHLVR

YVYKQCSEGGLVDVRIAARHYCGNVIRKMVFNKRFFGKGKKNGGPGFEEAEHVDALFRIL

DYSFSFCLSDYLPCLEGFDLGGHEKIIKEANGIIGKYHDPIIEGRVQQWKDGSKKEEEEL

LDVLITLKDDNGNPLLSTDEIKAQIT (0)

 

>CYP79D JcCA0255421.10 -  exon 1

78% to JcCA0009561.10

67% to CYP79D28   gi|255564896|ref|XP_002523441.1  Ricinus communis

MSFSSQAILLPIIILFIYCLIKSHKPPSEDRKAKQQLPPGPKPWPVIGCLPAMLTNKPTF

RWINNLMKQMDTEIACVRIGNVHVIPVSCPKISCEFLKAQDSNFATRPITMSTRVTSKGY

LTTVLTPYGDQWKKMKRILVTQMLSPAKQLWFSGKRVEGADHLVRYVYKQCSEGGLVDVR

IAARHYCGNVIRKMVFNKRFFGKGKKNGGPGFEEAEHVDALFRILDYSFSFCLSDYLPCL

EGFDLGGHEKIIKEANGIIGKYHDPIIEDRVQQWKDGTKKEEEDLLDVLITLKDDNGNPL

LSTDEIKAQIT (0)

 

>CYP79D JcCA0101361.10 – pseudogene

BABX01000167 two frameshifts, probable pseudogene

89% to JcCA0009561.10
67% to CYP79D29   gi|255564890|ref|XP_002523438.1  Ricinus communis

SFSFITIMMGTITSLLIYSIKSHK

(deletion)

NVHVIPVTCPKISCEILKAQDANFDTRPISMSNRFTSKGYLTIALTTFGDQWKKMRT

VLVTEMLSTAKQQRFYGTRVEEANHLVRFVYNQCNEGGLVGVKVAAQNYCGNVIRKMMFN

KRFFGKGKKNGGPGFDEEEHIDAIFRVLHHLYAFCLSDYLPCLVGFDLHGHEKIIKEANG

IIGKYHDPIIDDRVQQWKDGTKKEEEDLFDVLITLKDDNGNPLLSTDEIKAQITEIMLST

VDNPSNAVQWAIAEMINQPKILEKAVEELDQVVGKE &

GLVQESDLSQLNYITACAREAFRLHTVVPFNIPHVSMTDTTVANSFT

PKGSHALLSRVGLGRNPKIWDDSHKFKPERHIKNDGSQVTVIEPYLNFI &

SFSTGRRGCPGLTI

 

>CYP79D JcCA0292601.10 +  exon 1

69% to CYP79D28 gi|255564896|ref|XP_002523441.1  Ricinus communis

MSFSSQAIPLPIIILFIYYLIKSHKPPSEDRKAKQQLPPGPKPWPVIGCLPTMLTNKPTF

RWINNLMKQMDTEIACVRIGNVHVIPVSCPKISCEFLKAQDSNFATRPITMSTRVTSKGY

LTTVLAPYGDQWKKMKRILVTQVLSPAKQLWFSGKRVEGADHLVRYVYKQCSEGGLVDVR

IAARHCCGNVIRKMVFNKRFFGKGKKNGGPGFEEAEHVDALFRILDYSFSFCLSDYLPCL

EGFDLGGHEKIIKEANGIIGKYHDPIIEDRVQQWKGGTKKEEEDLLDVLITLEDDNGNPL

LSTDEIKAQIT (0)

EIMSATVDNPSNAVEWAIAEMINQPKIFEKAAEELDKVVGKERLVQESNLSRLNYITACA

REAFRLHPVSPFNIPHVSITDTTVANYFIPKGSHVLLSRVGLGRNPKIWDEPHKFKPECH

IKNDGSGVTLTEPDLRFISFSTGRRGCAGVTMGTSITLMLFARLLQGFTWNVPRNE &

KSIDLKESEGDLTLAKPLVLLAKPRLPAYVYDQIMHDN*

 

>CYP79D JcCB0505001.10 +  exon 1

BABX01086645 
64% to CYP79D28 XP_002523441.1  Ricinus communis

MRIWTIGHFSTEMSFSHQTILLITIILLIYLIKSHQSPSQYKKAKLPLPPGPKPWPIIGC

LPEMSKNELAFRWIHHLMKQMNTEIACFRLGNVHVITVTCPNISCEFLKAQDSNFATRPI

TISSGVTSKEYLTTALTPCGDQWKKMKRILVTQMLSPAKQLWFYEKRVEEAEHLVRYVYK

QCSEGGLVDVRIAARHYCGNVIRKMVFNKRFFRKGKKNGGPGFEEEEHVDALFRILDYIF

SFCLSDFLPCLKGFDLGGHERIIKEANGIIGKYHDPIIEDRVQQWKNGAKKEAEDLLDVL

ITLKDDNGNPLLSGDEIKAQIT (0)

 

>CYP79D JcCA0224261.10 – runs off at YGN

BABX01013942 one frameshift at &

64% to CYP79D28 XP_002523441.1  Ricinus communis

MENCYGFAAIGFSTTPFSFSFITILMGTMTLLL &

FIKSNKPPSQDEKSILRQQLPPGPKPWPIIGCLPE

MSKNEPAFRWIHHLMKQMNTEIACFRLGNVHVITVTCPNISCEFLKAQDSNFATRPITMS

SGVTTKEYITTVLTPCGDQWKKMKRILVTQMLCPAKQLWFYGKRVEEAEHLVRYVYKQCS

EGGLVDVRIAARHYCGNVIRKMVFNKRFFGKGKKNGGPGFEEEEHVDALFRILDYIFSFC

LSDYLPCLKGFDLGGHEKIIKEANGIIGKYHDPIIEDRVQQWKNGAKKEAEDLLDVLITL

KDDNGNPLLSGDEIKLLNYGN

 

>CYP79D BABX01089614, runs of the end

64% to CYP79D28 XP_002523441.1  Ricinus communis

MENCYGFAAIGFSTTPFSFSFITILMGTMTLLLIYFIKSNKPLSQDEKSILRQQLPPGP

KPWPIIGCLPAMYKNEPAFRWIHNLMKQMNTEIACIRLGNVHVIPVTCPKISCEILKAQD

ANFATRPISMSTRFTSKGYLTTTLAPFGDQWKXMRXVLVTEMLSXAKQRRFYGTRVEEXN

HXVRYAYNQCNEGGLVDVRVAAQHYCGNVIRKMMFNKRFFGKGRKNVGPGFEEEEHIDAI

FRVLHHLYAFCLSDYLPCLAGFDLDGHEKIIKESNGIIGKYHDPIIEDRVQQWKDGTKKE

EEDLLD

 

>CYP79D JcCB0612561.10 + /pseudo (two stop codons and a frameshift)

BABX01094105 runs off the end at HGFT

92% to CYP79 JcCA0009561.10
68% to CYP79D29 XP_002523438.1  Ricinus communis

MENCYGFAAVGFSTTP

FSFSFITILMGTMTLLLIYFIKSNKPPSQDEKSILRQ**PPGPKPWPIIGCLP

AMYKNESAFRWIHNLMKQMNTEIPCIRLGNVHVIPVTCPKISCEILKAQDANFATRPISM

STRFTSKGYLTTTLAPFGDQWKKMRGVLVTQLLSPTKQRRFYGTRVEEANHLVRYAYNQC

NEGGLVDVRVAAQHYCGNVIRKMMFNKRFF &

GKGRKNVGPGFEEEEHIDAIFRVLHHLYAFCLSDYLPCLVGFDLDGHEKIIKEANGIIGKYHDPIIEDRVQ

QWKDGTKKEEEDFLDVLITLKDDNGNPLLSTDEIKAQIT (0)

EIMLATVDNPSNAVERATAEMINQPKILEKAVEDLDRVVGKERLVQESDLSQLSYITA

CAREAFRLHPVVPFNVPHVSMTDTTVANY

FIPKGSHVLLSRVGLGRNSKIWDEPHKFKPERHIKNDGS

QVKLVEPYLNFISFSTGRRGCPGVTMGTSMTLMLFARLLHGFT

 

CYP80 family (6 sequences, maybe 6 genes, 3 are complete with three more C-term pieces)

 

>CYP80C JcCA0128991.20 - 

63% to CYP80C1 Populus trichocarpa
65% TO CYP80C5 EEF51432.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis
87% TO CYP80C   JcCA0261201.20, 84% TO JcSR157561r.10   – mid region, pseudogene
complete
BABX01003289 first gene

two genes on this contig (-) strand

second gene JcCA0128991.10 runs off the end at HCFD

MDQTLLYLPFLVFLPIFFIFLKKICFPPLKQGRLPPG

PRPWPILGNIPHIGKKLHISLTNIAKVHGPLISLRLGNQLVIVASSSKAAIEILKYNDRL

LSARSVPKAVPRKSHVLERISLVWNPTCNEQWKSFRALCRTELFSAKAVESRASLRENKM

TEMVEYLSSKEGEEVNIGEVVFATVFNTISNLLFSKDLISLQNQGLKSFLWKMMELGAAP

NVADFYPTFAELDPQGIRRAISECLKEMFGIWEIYIKERRERHANAHHVSSTDFLDVFLS

NGLDDDQINWLVM (0)

ELFSAGVDTTTTTVEWAIAELLKNKQAMKTIQQELERDINRNPIQ

ESQVCQLQYLNACVKETLRLHPPAPLMIPHCSTEACELMNFVVPKNTRVLVNVWAIGRDP

FIWEDSDLFKPERFLDSGLDFKSQNFDFLPFGSGRRICPGLPMATRQLPLILASLIYCFD

WSLPNGEDPALLDMNDKFGITLQKEQPLLIVPKRKL*

 

>CYP80C JcCA0128991.10 -  C-term PSEUDOGENE

BABX01003289 second gene runs off the end at HCFD

no ESTs to extend seq.

67% TO CYP80C5 EEF51432.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis
80% TO CYP80C   JcCA0128991.20
91% TO JcSR422361f.10   + I-helix to PERF, possible pseudogene, NO EXON 1 UPSTREAM

ELFSAGVDTTTTTVEWAMAELFKSKQSMATLQQELNTKINRNPIQESQVSQLPYLNACAK

ETLRLHPPVPFLVPHRSTEPCEIMDCNIPENTRVLVNVWAIGCNPFIWEDSELFKPERFL

DSSLDFKSQNFDFSPFGLGRRMCPGLPMATRQLPWILASLIHCFD

 

>CYP80C JcSR157561r.10 – mid region, pseudogene

2 frameshifts, one stop

84% to CYP80C JcCA0128991.20, 82% to CYP80C JcCA0261201.20
63% to CYP80C6 XP_002513351.1 (S)-N-methylcoclaurine 3'-hydroxylase isozyme Ricinus communis

BABX01119759 runs off the contig at both ends

No ESTs

FSFLVSLPIIFIVLKQICFLPSKPRLLPPGPRPGPIVGNIPH &

IGKKLHISLTNIAKVHG

PLISLRLGTQIVIVASSSKAAIEILKDNDRLLSARSVPKAVPHKSHVLERISLVWDPTCN

DHRKSFRAMCRTELFSAKAVESQAALREKKMTEMIEYLSSKEGEVVNLGDI &

VFATVFNTISNLLFSKDFISLQNQELKSLLWRMMELGAAPNLADFYPTFAELDPQGIRRE

IS*CLKEMFGLWEIHIRERRERYANAHNASNTDFLDVFLSNGFDDDQINWLAM

 

>CYP80C JcSR422361f.10 + I-helix to PERF, possible pseudogene

BABX01120392 runs of the end at FLD

67% to CYP80C1 Populus trichocarpa
62% TO CYP80C5 EEF51432.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis
91% to JcCA0128991.10

ELFSAGVDTTTTTVEWALADLLQNKQSMATLLQELNTKINRNPIQESQVSQLPYLNACAK

ETLRLHPPVPFLVPHR &

STEPYEIMDCNIPENTRVLVNVWAIGCNPFIWEDSELS*TERFLD

 

>CYP80C JcCA0261201.10 + 

62% to CYP80C1 Populus trichocarpa
complete
BABX01018063 first gene

JcCB0939001.10 +  C-term

JcCB0177411.10 -  N-term

64% TO CYP80C6   gi|255544580|ref|XP_002513351.1 (S)-N-methylcoclaurine            3'-hydroxylase isozyme Ricinus communis

MEQIISTRVFINLLSST

FFFVFLPVISFIFLKHVLISLKRHRFPPGPRPWPIIGNIPHVREKPHISMAKLAKLHGPI

FSLRLGYQLVVVGSSPAVAAEILRKKDRFLSGRFVPKAIPRPSHELDRIALVWNPSCSDQ

WKSLRALSRTELFSAKAIESKAALREQKVSEMIEFLGIKQGQVVNVGEVVFATVFNSISN

LLFSKDFISFEDEESVSGLKGLMWKLMELGSAPNLADFYPIFSGLDLQGLRKKMSKCLKE

MFDIWEIFIKERRERHARNGVVKEDFLDVFISNGFDDEQINWLIH (0)

ELFSAGTDTTTTTVEWAMAELLKNKEAMNKVCQELEREINKN

SINESQVSQFPYLYACIKETFRLHPPAPFLGHQPLETCEVMNYTIPKDSQIIVNIWAVGR

DPLAWEDPLLFKPERFLESSLDLKGHDFELLPFSSGRRMCPGLPMAIRFLPLILASLIHY

FDWSLPGGEDPAMLDMNDKYGITLQKEKPLLLVPNTKL*

 

>CYP80C JcCA0261201.20 +

BABX01018063 

FM894218 EST

62% to CYP80C1   Populus trichocarpa
complete
JcCB0936721.10 +  C-term

BABX01018063 second gene

two genes on this contig

87% to CYP80C JcCA0128991.20
same as JcCB0936721.10
64% TO CYP80C5 EEF51432.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis

MDQTLLYLPFLVFLPIVFIFFKKICFPPSKQCRLPPGPRPWPIV

GNIPHIGKKLHISLTNIAKVHGPLISLRLGTQLVIVGSSSKAAIEILKHNDRLLSARSIP

KAVPRKSHVLERLALVWNPTCNDQWKSFRTLCRTELFSAKAVEAQTALREKKMTEMIEYL

SSQEGEAVNIGEIVFATVFNTISNLLFSKDFMSLQNQGLKSLLWRMIELAAAPNVADFYP

IFVELDLQGIRRQMAKCLKEMFGICEIYIKERRERHANAHNASSTDFLDVFLSNGLDDDQ

INWLFM (0)

ELFSAGVDTTTTTVEWAIAELLKNKQAMKTLQQELETEINRNPIQESQVSQLLY

LNACVKETLRLHPPAPFLLPHCATEACELMNFVVPKNTRVLVNVWAIARDPLIWEDSELF

KPERFLHSGLDFKSQNFDFLPFGSGRRICPGLPMAIRQLPMILASLIHHFDWSLPNGEDP

ALLDMTDKFGITLQKEQPLFIVPKIK*

 

CYP80E partial sequences

 

>CYP80E10 JcCA0258831.10 +  C-term

BABX01017796

61% to CYP80C1 Populus trichocarpa
68% to JcCB0478711.10
51% to CYP80C   JcCA0261201.20

runs off the contig end at FSLK, missing 67 aa at N-term

no ESTs to extend seq.

76% TO CYP80E10 XP_002509713.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis. Note: there is only one CYP80E in Ricinus, so this is the ortholog

FSLKL &

GTQIVM*GSSEAAAMEILKTHDKILSGRYIPHVAPTNKSPELKNLSLGWTLECND

KWKYLRTICKSELFSAKALEAQQCKREEKVMEMIGFIKEMEGKEVKVREVAMVTVFNMLS

SIMVSKDLMSLDKQENGDGEMGLLLEKILELASTPNISDLYPILGRFDLQGLQKKIMELH

GRFFEICEGIVEERREEKRRGSILGSNQRDFIDTLIDNGSSNDQINVLLV (0)

ELLSASTDTSSTTIEWTMAELIRNPECMKKVKEEITKKLT

QQIITIKESHIPQLPYLQACVKETLRLHPPGPFLLPHRATDTCQLMNYTIPKNSQVLVNF

WAIGRDPTNWENPSVFNPERFLDLNLDFKGNDFGFIPFGSGRRICPGLPMAAKQVPLIIA

SLVHFFNWSLPNGKDFTDLNMAENFGLTLRIEQPLILIPKIKG*

 

>CYP80E JcCB0478711.10 -  C-term

BABX01085059

runs off the end at RKS

no ESTs to extend seq.

54% to CYP80C JcCA0261201.10
57% to CYP80E1 Populus trichocarpa
66% to CYP80E10 XP_002509713.1 (S)-N-methylcoclaurine 3'-hydroxylase Ricinus communis
65% TO JcCA0258831.10
yellow region not well conserved

RKSREWFKKICKISLVIINERREKNKGDSDKVQRDFLDALINNGSSNDQINMLLM (0)

EMFSAGTDSSSSSIEWMMAELIRNPKYMKKVEEEIQRETNNEENLDSHLHKFTFLQACFKETLRL

HPPGPLLVPHRATESCQVMNYTIPKNSQVMVNFWAIGRDPKIWEDPLEFKPERFLNSNLD

FKGNDFEYIPFSSGRRICPGLPMASKQVPLIVACLVHFFDWSLPNGQIRNAGQINMNEKY

GLTLMKEQPLLLIPKSKK*

 

 

CYP81 family (29 sequences, 9 are complete)

 

>CYP81B54 JcCA0147611.20 - 

58% to CYP81B1v1   Helianthus tuberosus
complete
75% to CYP81B54 XP_002510131.1  Ricinus communis (ortholog)
second best match is 

61% to CYP81B55 XP_002510130.1  Ricinus communis

MEISYFFSIFLVFVFLYFLSKHFLLINKNLPPSPGLSLPIIGHLHLFKKPLHRTFANLSN

KYGPILFLKFGSRPVLLISSPAAAEECFTKNDIIFANRPKLLAGKHLGYDYTTLVWASYG

DHWRNLRRIASIELLSSNRLQMLYNIRVEEVRSLVFSLFRGSKRGEFVAVDMKSMFFELS

LNVMMRMIAGKRYYGDNTKELDEERRFKEIVTETFQLSGATNIGDFIPVLNWIGLNKIKK

RLEALQRKRDRFMQDLIEEHKRQRCLDKEWKSKTMIDVLLRLQEDEPEYYTDEIIRGMMQ

VLLSAGSDTSAGTMEWAMSLLLNNPNTLIKARAEIDKNIGESKLIEESDLSKLPYLHCII

TETLRMQPAAPLILPHESSEQCTVGGFYVPRGTMLLVNTFAIQNDPNLWEEPSKFEPERF

EGLKGKKEGYVLLPFGAGRRGCPGEGLAMKMTGLALATLIQCFEWERISEEMVDMKEGNG

LTMPKAEPLFAKCRPRPTLLDLLSHF

 

>CYP81B55 JcCA0147611.10 – N-term

60% to CYP81B1v1   Helianthus tuberosus 
BABX01005370.1a (two genes on this contig)
Runs off the end of contig at RADE
Other gene is CYP81B54 JcCA0147611.20
67% to CYP81B54   JcCA0147611.20
76% to CYP81B55 XP_002510130.1  Ricinus communis (ortholog)
no ESTs to fill the gap

CYP81B55 exon 1

 BABX01113096a two genes on this contig

85% to JcCB0091661.10 
81% to CYP81B55 XP_002510130.1  Ricinus communis

MIFHLLLFLALYIITTHLLNKIRNLPPSPFPTLPIIGHLHLLSKPLHRSLSKISNRYGPV

LLLQFGFRRVLLVSSPSAAEECFTKNDIIFANRPRLLHGKHIGYSYTSLAWASYGDH

WRNLRKLAAVELLSSYRLHLLSNIRADE

 

TLLAAGTDTSATTMEWAMSLLVNNPEILEKAQNEIDIVVGNDNLVAESDIPKLPYLHCII NEVMRLYPAGPLLLPHESSEEGSIGGYRVPRGTMLLVNLWCIQNDPGLWEEPTKFKPERF EGCHEGVRDGFKFMPFGSGRRSCPGEGLALRMIGLTLGSLLQCFEWKRVGKEMVDMTEGL GLTMPKAQPLLVECRPRPSMANLLSQV*

 

>CYP81B JcCB0091661.10 +  pseudogene

BABX01113096b two genes on this contig

56% to CYP81B54   JcCA0147611.20
64% to CYP81B55 XP_002510130.1  Ricinus communis

MIFHLLLFLVLYILATHILNKILNLPPSPFPTLPIIGRLHRLGKPLHRS LYKISNRHGPVLLLQFGSRRVLLVCSPSTVEECFTRNDIVFANRPSLLPGKYMGYNFTTL TWASNGDHWRKLRKLAAVEILSSHRLQLLSDIRA &

EEVKSMIYKLAAKPNQAVDMRSLFFELTFKIIMRI &

IGGKKYYGEEAQS &

LDEAWRFHDITIETMRLGDEIFIKDCLPLWGGLDSDEKRMN*LNCRKKRDEFMQDLID

EHRRKTGDDCFLLSARKKIMVEVLLSLQEQEPE*YKDEIIXXXXX

VLVAAGIDTSSATMEWAMSLLVNNPEILEKAQSEIDIVVGRDNLVAE

SDIPKLPYLRCIINEVMRLYPVGLLLHHESSEECSVGGYRVPRGTMLLVNLWGIQNDPRI

WEEPRKFKPERFEGCRDGVRDGFKCMHFGLGKRSCNGEGMALRMIGLTLASLLQCFEWKR

VGKEMVDVTEGGGLTIPKAQPLLVECRPRPSMVNLLSQV*

 

>CYP81C14 JcCA0309481.10 - 

64% to CYP81C3 Populus trichocarpa
73% to CYP81C14 XP_002510136.1  Ricinus communis (ortholog)
next best is 52% to CYP81B55 XP_002510130.1  Ricinus communis

complete

MESLYYYLGLFLFIFLFYKILSHHRQNLPPGPLALPVIGHLHLIQKPLPLALQTLQSKYG

PILFLKFGCRSVLVVSSPAAVEECLAKNDIIFANRPRSMAGDHFTYNYSSFVWAPYGDLW

RILRRFTVVEMFSSKSLQKTAAIREEEVARLLRRLFKFSAGGKQNVDLKFLSSVLMCNVV

MRMTVGKRYVEEEVEGTELEKQLFQEFKETFFPSLTLNICDFIPILRMTGFKGIEKGMIK

LQRKRDEHLQNLLDEIRLKTRNSGTSNEEDKSLIETLLSLQELEPEFYTDEVIKTIMLIT

LIAGAETSAVTLEWVISLLLNNPEVLKKVKAEIDNHVGNRHLLNELDLVKLPYLRCVINE

TLRLYPAGPVLLPHLSSENCTVGGFEIPGGTMLLVNVLAMHRDPKVWEEPYEFKPERFEG

DLGEHSQQGFRFIPFGMGRRACPGAGMGIRMVSVALGALIQCFEWENPGLEEVDMSQTFG

ISLSKTKPLVALCSPRQELVELLSQL

 

>CYP81S JcCA0044911.10 + 

65% to CYP81Q7   Vitis vinifera
73% to CYP81S17 XP_002524942.1  Ricinus communis
next best 68% to CYP81S18 XP_002524941.1  Ricinus communis

MRICMTKLFWIILYKPISFGTFASGNNNPRKTKNMEHNTVLYFFSSFLLLFLVIKFYHSK

VHRKNLPPSPPSLPIIGHLYFLKPPIHRILYSLAQKYGPIISLRFGSRPVIVVSSSSIAE

ECFTKNDIVFANRPRFLLGKHIAYNYTTISQAPYGDHWRNLRRVGAIEIFSNHRLNMFLS

IRRDEINRLIMKLSLNSMQDFCKVELKSMLQELTFNIMMRMVAGKRYYGDDVSNEEEGKE

FRKLMKEVLAYAGASNPADFLPILHWIDGGYLKKRIIGFANRFDKFFQGLIDQHRSKKGN

LENKNTMIDHLLSLQESEPEYYTDEIIKGLIQVMLFAGTDTSAVTLEWAMSNLLNHPSSL

RKAREEIDIQMGTESLIDEPDLPTLLYLQNIISETLRLYPAAPLLLPHVSSEDCKIEGYD

VPRGTMLLVNAWAIHRDPTLWDDAMSFKPERFDKGEEEAKKLMPFGLGRRACPGAGLAQR

VVGLALGSLIQCFEWERVSDKEVDMKEGRGSTMPKAEPLEAMCKARPIIHK

 

>CYP81S JcCD0072850.10 -   

BABX01142436.1

96% to CYP81S JcCA0044911.10
78% to CYP81S17   gi|255567929|ref|XP_002524942.1  Ricinus communis
no ESTs

DTSAVTLEWAMSYLLNHPSSLRKAREEIDIQMGKESLIDEPDLPKLLYLQ &

NIISETLRLN

PAAPLLVPHMSSEDCKIEGYDVPRGTMLLVNAWAIHRDPTLWDDAMSFKPERFDKGEEEA

KKLMPFGLGRRACPGAGLAQRVVGLALGSLIQCFEWERVSDKEVDMKEGRGSTMPKAEPL

EAMCKARPIIHKLI*

 

>CYP81S JcCA0152811.10 +

BABX01005963.1a

64% to CYP81Q7   Vitis vinifera
73% TO CYP81S17 XP_002524942.1  Ricinus communis
88% TO CYP81S   JcCA0044911.10

complete

MEHYTVLYFSSSFLLLFLVIKFCQSKVHRKNLPPSPPSFPIIGHLYLLKPPMHRILHSLA

QTYGPVIFLRFGSRPLIIVSSLSIAEECFTKNDIVFANRPRLLMAKHISYNYTTMTQAPY

GDHWRNLRRVGAIEIFSAHRLNTLLSIRKDEIKRLIMKLSINSMQDFCKVELKSMFQELT

FNIMIRMVAGKRYYGDDVSNEEEAREFRKLMKKVVAYAGASNPADFLPILNWIDGGYLRK

RIKGFAKRFDNFFQGLIDEHRSKKGNLENKNTMIDHLLSLQESQPEYYTDEIIKGLIQVM

LFAGTDTSAVTLEWAMSYLLNHPSSLRKAREEIDIQMGKESLIDEPDLPKLLYLQNIISE

TLRLNPAAPLLVPHMSSEDCKIGGYDVPRGTMLLVNAWAIHRDPKLWDDAMSFKPERFDK

GEEEAKKLVPFGLGRRSCPGAGLAQRVMGLTLGSLIQCFDWERVSDKEIDMAEGRGVTMP

KAEPLEAMCKARPIIHKILV

 

>CYP81S JcCB0769781.10 +  

BABX01104552.1 runs off the contig at both ends

76% to CYP81S JcCA0152811.10
73% to CYP81S17 XP_002524942.1  Ricinus communis

no ESTs to extend seq.

RKDEIKRLIII &

LSHNGMQDFSKVELRSRFLELTFNIMIRMIAGKRYYGDDVSDEEEAREF

RKIMKEAFAYGDVTNPGDFLPIYNWIDGGAFEKGMIRIAKRCDEFYVGLIDEHRNSKGNL

QNTNTMIKHLLSLQESEPEYYTDEVIKGLIQ (0)

VILFAGTHTSAVTLEWAMANLLNHPNAVR

KARDEIENLIGENSLLDEPDLPKLPYLQNIVSETLRLYPTVPLLVPHMSSEDCTVGGYEV

PRGTMLLVNAWAIQRDPTLWDDAMSFKPERFDK

 

>CYP81S JcCA0152811.20 +  pseudogene missing part of exon 2

BABX01005963.1b (three genes on this contig)

71% to CYP81S18 XP_002524941.1  Ricinus communis

MEDSLHYCFLSLVFLIFAFKFYQSKISHKNLPPSPPSLPIIGHLRLLKPPAPMHRTLHHL

SKKYGPIIFLRFGSRPVVVVSSTSAVEECFTKNDIVLANRPNFFLIGKHISYNNSTIGQS

PYGDHWRNLRRIGTIEIFSTHRLNAFLSIRKEEIKRLAVKLSHESIQDYAKVELKSKFKE

LTFNVIMRMIAGKRYYGEDVTDKEEGKRFRKIIGEIMSTGGVSNPGDFLPVLNWIDGGKF

EKKVIRLGKEVDKFLQDLIDEHRSKKKNLESMNTMIDHLLTSQESEPEYYTDEIIKGLLL (0)

 

WDDPLSFKPERFDNGEGESFKLMPFGLGRRSCPGAGLANRVVGLALGTLIQCFEWKRVTE

EEVDMVEGRGLTMPIAEPLEAMCKARPIMKQTLSQPV*

 

>CYP81S JcCA0152811.30 +

BABX01005963.1c

61% to CYP81Q7   Vitis vinifera
72% TO CYP81S18   gi|255567927|ref|XP_002524941.1  Ricinus communis
70% TO CYP81S   JcCA0044911.10

complete

MEDTVTYLFLSAIILIFAFRLHRSRICLKNLPPGPLSLPIIGHLHLLKPPMHRTLHSLAQ

KYGPIISLRFGLRLVIVVSSSSAVEECFTKNDIVLANRPEFLIGKHIAYNNTTMAQSSYG

SHWRNLRRIATIDIFSTHRLNKFLSVRKDEIQRLIVKLSHGSVQNFAEVELKSMFKELTF

NVMVRMIAGKRYYGEDVSDEEEARQFREMMCEIISYAGASNPIDFLPFLNWIDGGRFEKK

VKRLGKQIDKFLQDLIDEHRSKKDHLESINTMVDHLLSLQESEPGYYTDEIIKGLVLNIL

FAGTDTSSVTLEWAMSNLLNHPSKLRRARDEIDNQIGQDCLIDESDLSKLPYLQKIILET

LRLYPAAPLLVPHMSSDDSTIGGYDVPRGTILLVNAWAIHRDPTQWDDPSSYKPERFGNG

EEENFKLMPFGLGRRSCPGDGLAHRLMGLTLGLLIQCFEWKRTTEEEVDMVEGRGLIMPK

AKPLEAMCRARPILNKIV

 

>CYP81S JcCA0058601.20 - 

58% to CYP81Q7   Vitis vinifera
67% to CYP81S16   gi|255564958|ref|XP_002523472.1  Ricinus communis
62% to CYP81S19   gi|255567921|ref|XP_002524938.1  Ricinus communis

MEDKYFYPVIFVLPFLFLALRTTTKQKRKAPPSPFSLPIIGHLHLIKLPMHQTLHNLSQK

YGPIISLRFGSQKVMVISSPSAVEECLAKNDIVFANRPPFTLSKYVGYDNSTLSTSFYGD

HWRNLRRIGMLEVFSSNRLNAFSGIRREEIKIFLKKLYDISSNGFAKVELKPLLMELTFN

MIIRMIAGKRYYSEETTGKEKEEAKQFKETIEETFEYAGASYLGDFLPILQWIDYGGFLK

RAERLGKRTDRLWQSFIDEHRKKEIKNTMISHLLSLQESEPEYYTDEVIKGLIL (0)

DIVFGGTESTAITIEWAMSNLLNDPEVLEKAKKELDTKIDDKESLMDETVVSKSPYLQSII

TETLRLHPPGPLLIPHLSSQECNVGGYSVEPNTMLLVNAWAVHRDPKLWDDAAKFKPERF

ESGTNQGIEANKLLPFGLGRRSCPGIGLANRVVGFGLGSLIQCFEWRRLSHLEIDMSEGI

GLTMPKAQPLVAMCKARDIMTKALSSSP

 

>CYP81S JcCA0286101.10 + 

BABX01020837 exon 2

96% to CYP81S   JcCA0058601.20, 98% to JcCD0049914.10
72% to CYP81S16   gi|255564958|ref|XP_002523472.1  Ricinus communis
No ESTs

DIVFGGTEATAITIEWAMSNLLNHPEILEKAKKELDTKIDDKESLMDETVVSKSPYLQSI

ITETLRLHPPGPLLIPHLSSQECNVGGYSVEPNTMLLVNAWAVHRDPKLWDDAAKFKPER

FESGTNQGIEANKLLPFGLGRRSCPGIGLANRVLGFGLGSLIQCFEWRRVSDLEIDMSEG

IGITMPKAQPLVAMCKARDIMTKALSSSP*

 

>CYP81S JcCD0049914.10 -  

BABX01139765

96% to JcCA0286101.10
72% to CYP81S16 XP_002523472.1  Ricinus communis
runs of the end at SDL

DIVFGGTESTAITIEWA

MSNLLNHPEVLEKAKKELDTKIDDKESLMDETVVSKLPYLQSIITETLRLHPPGPLLIPH

LSSQECNVGGYSVEPNTMLLVNAWAVHRDPKLWDDAAKFKPERFESGTNQGIEANKLLPF

GLGRRSCPGIGLANRx*GFGL &

GLLIQCFEWRRVSDL

 

>CYP81S JcCB0168221.10 +  pseudogene

BABX01064000.1

Runs off the contig end

95% to CYP81S   JcCA0058601.20
96% to CYP81S   JcCB0810301.10
65% to CYP81S16 XP_002523472.1  Ricinus communis

MEDKYFYLVFFVLPFLFLALRTTTKQKRKVPPSPFSLPIIGHLHLIKLPMHQTLHNLSQ

K*GPIISLRFGSQKVMVISSPSAVEECLAKNDIVFANRPQFTLSKYISYDNSTLSTSFYG

DHWRNLRRIGMLEVFSSNRLNAFS &

GIRREEIKIFLKKLYDISSNGFAKVELKPLLMELTFNMTIRMIAGKRYYSEETTGKEKEE

AKQFKETIEETFEYAGASYLGDFLPILQWIDYGGFLKRVERLGKRTDRLWQSFIDEHRKK

EIKNTMISHLLSLQESEPEYYTDEVIKGLIL (0)

DIVFGGTETSAITIEWAMSNLLNHPDVLEKAKKELDTKIDDKESLMDET

VVSQLPYLQSIITETLRLHPPAPLLAPHLSSQECNVGGYSV

 

>CYP81S JcCB0548761.10 - pseudogene

BABX01089420.1

49% to CYP81Q7   Vitis vinifera
56% to CYP81S   JcCA0058601.20
58% to CYP81S19 gi|255567921|ref|XP_002524938.1  Ricinus communis

MENTMLLVCSLSFIFLLLSLKLWSYTTSHNTKLPPS &

SPALPVIGHLYLLKQPLHRTLDNLSKTYGSIFSLQCGSRFMIVVSSPSFV*ECF

TKKDIIFANRPFLTIGKNVIYDQNLNTLITSPYGEHWRQLRRIGTLEI &

FSTNRLNGFIGIRREEIRIFLKNIYKVCNDGSDKVEIKPLLSELVFNIM

MRMITGKRYCESKVLVNEIFRYTEVSYSGFFLPILQWVDAKGNLKKVKQLAKTSYELMQQ

LIDEQKNDKKGSNDSVIGHLLSLRQLELEYYTDEIIKALIM (0)

DIISAGTESSITTMEWTMS

KLLNNQQMLEKAKKELDSKIGQERLIEESDISNLPYLQNIISECLRLYPTGPLLLPYMSN

EDCRVGGYYVPKNTVLAVNAWALHRTPKLWDDASTF*PERFEGDKR*GLQDKFLPFGLGR

RACLGMGLANRATAFALGSMIQCFEWKEWK &

ERMEGEEIDMSEAIGFTVSKCKPLVAICKARDVIHKELSMLDLS

 

>CYP81S JcCB0810301.10 – exon 1

BABX01106726.1

98% to JcCA0058601.20 (3 aa diffs)
no ESTs to extend seq.
64% TO CYP81S16 XP_002523472.1  Ricinus communis

MEDKYFYLVIFVLPFLFLALRTTTKQKRKA

PPSPFSLPIIGHLHLIKLPMHQTLHNLSQKYGPIISLRFGSQKVMVISSPSAVEECLAKN

DIVFANRPPFTLSKYVGYDNSTLSTSFYGDHWRNLRRIGMLEVFSSNRLNAFSGIRREEI

KFFLKKLYDISSNGFAKVELKPLLMELTFNMIIRMIAGKRYYSEETTGKEKEEAKQFKET

IEETFEYAGASYLGDFLPILQWIDYGGFLKRAERLGKRTDSLWQSFIDEHRKKEIKNTMI

SHLLSLQESEPEYYTDEVIKGLIL (0)

 

>CYP81S JcCB0003611.10 –

60% to CYP81Q7   Vitis vinifera
64% to CYP81S18   gi|255567927|ref|XP_002524941.1  Ricinus communis
66% to CYP81S JcCA0044911.10

complete

MEVIISYLYTSLSLLLLLFLSYKFYLSKTTYKNLPPSPPSSLPILGHLHLLKPPIHRFLQ

SLSQKYGPIISLKFGHRIVVVVSSPSAVEECFTKNDIVLANRPQFTLSKHINYNCNDIIS

VPYGDHWRNLRRIGTLEIFSTNRLNEFTSTRKDEIKRLLIELSRHSKQDFAEVELKSRFS

NLTFNVILRMVAGKRYYGDDANNKEEAKEFRELIGEIFRNGGASHPEDFLPILSWINGGK

LEKTAKGLAKRSNKFLQGLIDEHRHRKEGSEITDTMIDHLLSLQGSEPNVYTDDVIKGLI

MIMLLAGTDTSSVTLEWAMSNLVNHPDTLNKAKIELDAIIGQEHLLEESDLSKLPYLHNI

ISETLRLYPSAPLLVPHFTSDDCTIGGYNVPRGTIVLVNAWAIHRDSSVWDDPISFKPER

FEQGQTEAYKYLPFGLGRRACPGATMAQRVVGLTLGSLIQCFEWRRIDEKLVDMTEGIGI

SMPKAEPLKVMCKSRDIMNKVLIETS

 

>CYP81S JcCB0122661.20 - 

55% to CYP81Q7   Vitis vinifera
59% TO CYP81S20   gi|255538150|ref|XP_002510140.1  Ricinus communis
81% TO JcCB0480461.10

complete

MNEIWFYFLSTFSLIFLLSKLFSLKQTQHKKIPPRPPAIPIIGHLHLMKEPLHRTLQNIT

DQYGPIVSLSFGSRNIVVVSSPNLLEDCFHKNDLLFANRPHLSAFKYLGCDYTTLGTSPY

GDHWRNLRRIASVQIFSTNRLNNFDSIRQDEVRILLKSLFANSQTGFTKVEMKSRLSGLS

FNIISRMISGERYFGTEVENLEVAKRFGDILKQTAEVSGGGASATLNLGDFLPFLRWVDF

RRIEKRLLRLQKNLDEFCQSLIDEHRKNNNSSSQEQGRPKTMIGKMLSLQESEPESYSDE

IIKGFILVMLLGGTGSAVAVEWAMSLLLNHPEVLKKARAELDKIIGQERLVGEDDFPKLP

YLQSIINETQRLYPVAPLVTPHESSDDCFIGGYYVPRGTMLLVNAWAIHSDPTVWNDPTK

FRPERFEGLDKDAHKYKLIPFGLGRRACPGAPLANRVMSLTLASLIQCFEWERPSEEQID

MCPGTGDTMPKSKPLEAMCRARESMINILHEL

 

>CYP81S JcCB0043451.20 + exon 1

BABX01081785 exon 1

BABX01079139  exon 2 = JcCB0393371.10   -
56% to CYP81Q7   Vitis vinifera
80% to JcCB0023541.20, 78% to CYP81Q JcCB0122661.20
60% to CYP81S20 XP_002510140.1  Ricinus communis
EST GT978449.1

MDEIWLYFLSIFSLIFLLSKLFSLK

QASHKKFPPSPPAIPIIGHLYLMKEPLHRTLQNMSNKYGPIISLSFGSRNVVIISSSNLL

EDCFNKNDVVFANRPHLAAFKYIGYNYTTLGTSSYGHHWRNLRRIATLEIFSTTRLNSFQ

SIRQDEVRILLKNLFANSQTSFTKMEMRSRLSGLSFNIILRMISGKRYFGAEVENLEVAK

RFGDIARETTAVSGASNPGDFLPFLQWIDFKGLKKTMVRLHKDWDEFCQGLIDEHRKNKN

SSSQERGRSKTLIDNMLSLQESEPKSYSDEIIKGLIL (0)

NLIIGGTETSSVTMEWAMSLLLNNPEVLKKARAELDEIIGQDRLVDESDFSKLPYLQSIINE TLRLYPVAPFLVPHESSDDCTIGGYYVPRGTMLLVNAWAIHRDPTVWSDPTKFKPERFEG LGNEAYKLIPFGVGRRACPGAGLANRVMGLALAELIHCFEWGRPSEELIDMTEGTGGTMP

KSQPLEAMCRARESMINILQDL*

 

>CYP81S JcCB0480461.10 +

BABX01085177.1 runs off the end at both ends

60% TO CYP81S20 XP_002510140.1  Ricinus communis
81% TO CYP81S   JcCB0122661.20
no ESTs t oextend seq

VPVMLSLYHPQAYLKTASTKTTLCLPIGLALPLSNIQVYNYTTLATSSYGDHWRNLRRIA

ALQIFSTNRLNYFHSIRQDEVRILLKNLFANSQTSFTEIDMKSRLSSLSFNIILRMISGK

RYFGAEVEILEVAKRFDDILRETAEVNGASNPGDFLPFLQWIDFQGMEKRLLRLQKNWDE

FCQGLINEHRNSNNSSSQEQGRSKTFIDKMLYLQESEPESYSDEIIKGFMLVMIVAGTDT

SALTMEWAMSLLLNHPEALKKARAELDKIIGQDRLVDESDYSKLPYLQSIINETLRLYPV

APLLLPHESSNDCLVGGYYVPRGTMLLANAWAIHRDPTVWNDPTKFRPERFEGLDKDAHK

YKFIPFGLGRRVCPGIPLANRVMGLALAALIQCFEWERPSEEQIDM

 

>CYP81S JcCB0023541.20 +  exon 1

BABX01068449

95% to CYP81S   JcCB0480461.10
80% to CYP81S   JcCB0043451.20 + exon 1
54% to CYP81Q7   Vitis vinifera
55% to CYP81S20 XP_002510140.1  Ricinus communis
no ESTs to extend seq

MDEIWFYFLSIVSLIFLLEKLFSLKQTWHKEFPPSPPAIPIIGHLHLLKEPLHRTLQDIS

NKYGPIVSLSFGSRNVVVVSSPSLIEDCFHKNDIVFANRPRLAAFKYTGYNYTTLGTSSY

GDHWRNLRRIAALQIFSTNRLNYFLSIRQDEVRILLKNLFANSQTSFTEIDMKSRLSSLS

FNIIFRMMSGKRYFGAEVENLEVAKRFDDILRETAELNGASNPGDFLPFLQWIDFQGMEK

RLLRLQKNWDEFCQGLINEHRNSHNSSSQEQGRSKTFIDKMLYLQESEPESYSDEIIKGFLL (0)

 

>CYP81S JcCB0130261.10 +  pseudogene

BABX01061655.1a

48% to CYP81B1v1  Helianthus tuberosus
57% to JcCB0480461.10
53% to CYP81S20 XP_002510140.1  Ricinus communis

HPEVLHKARE

EIDVNIGQSRLLDDSDLSKLPYLHSITNETIRLRSIGIISP*ESLEECIIGGYKIPSGTM

LLVNAWALHRDPEFWEDPDAFKSGRFQGVKQDREVLNSYNLVFFRRQCPGRGLAIR

 

>CYP81S JcCA0205711.10 + 

BABX01011864.1 exon 1

58% to CYP81S JcCA0058601.20
71% to CYP81S19 XP_002524938.1  Ricinus communis

No ESTs to extend seq

MEGFLPIVSVLSFLFLLFKLWSHNKRTQLKLPPTPPALPVIGHLHLFKQPIHRNLQNLSQ

KYGSIFSLRCGSYPMIIISSPSAVEECFTKNDIVLANRPPLLMGKYLNYNHTTLVTAPYG

DHWRNLRRISALEIFSAKRLDTFLGIRREEIRIFLQKLNQACCDGFSKVEMRPLLSELTF

NIIMRMVTGKRYGNYDVEEARKFREMIREIFAYAEASYPGDFLPFLQWIDFQGYVKKVKA

LARRTDELLQGLIDRQRNDQNSSNKSMISHLLSLQESQPEYYTDEIVKGFIL (0)

 

>CYP81S JcCA0298971.10 + pseudogene

BABX01022282.1

65% to CYP81S JcCB0003611.10
55% TO CYP81S18 XP_002524941.1  Ricinus communis

LQKYGSIISLKFGQHLIVIVSSPSAIEECFTKNDIILANRPLLTIFRQFSYNFNNIITIP

YGDHLRNLRHIGSVEIFSMNRLNKFIDIRQDEIQKLLINLSRHSK*DFVEVELKSTFSNL

TFNVIMGLVVGKRYYDDDMNGEEEAKQFRELISEIFGNAGTANLKDYLAILRWINDGKLA

KKAKELAERTHKLFTRLIDKHQQRKDGLKIADTMINHLLSLQESQPNFYTNDVINGLTM

 

>CYP81S JcCB0584681.10 +  

BABX01091862.1

66% to CYP81S JcCA0044911.10
68% to CYP81S16 XP_002523472.1  Ricinus communis
no ESTs

LEKAKDELV &

LKLGSESLMDELDISKLFYLQNIISETLRLYPAGPLLLPHLSSKDCTIEG

YNVSKNTIVLVNAWAIHRNPNLWNDAEKFKPERFEEGESDAYCYKFMPFGHGRRTCPGMG

LANKVVGFALGCMIQCFEWERVDGKEIDMTEGIGLSMPKAIPLEVMCKAREIMKVYSSL*

 

CYP81T

 

>CYP81T2 BABX01061655.1b N-term

Runs off the end

61% to CYP81T3 XP_002510139.1  Ricinus communis
66% to CYP81T2P XP_002510137.1  Ricinus communis
59% to CYP81T JcCA0299491.10
EST GW877928.1

CYP81T JcCB0300631.10 – C-term

BABX01072891.1 exon 2

61% to CYP81S JcCA0152811.10
61% to CYP81S JcCB0043451.20 
65% to CYP81T3 JcCA0299491.10
66% to CYP81T2P Ricinus communis (probable ortholog)
67% to CYP81T1 Populus trichocarpa
63% to CYP81T3 XP_002510139.1  Ricinus communis
no ESTs to extend seq

MEVANWLTLGLLLTLIFLSKFIFSKYENNKNYPPTPPSLPIIGHLHLLKEP

VHRALHDLSAKYGDVLLLRFGICKVLLVSSPAAVEECFTKNDVIFANRPRWLAGKHLNYN

YRTVGTTPYGARWRKIRRLLALEVFSTSRLAMFANIRQEEVQLLLKELFQHSIGKKEAVK

ANLSSKFVDLSFNVMMRMAAGKRY
 

VLLVAGSESSATT

MEWTMSLLLNHPETMKKVVTEIDTYVGFNRLLDETDLSKLTYLQNVINETFRLYPTFPLL

LPHESSDDCSICGFDVPKGTVLLVNLWTIHRDPKLWVEPTKFLPERFESEESDGYKLIPF

GIGRRACPGAVLARKVMVLALGALIQAFEWGRINNEEINMAQGNGLTMPKAEPLEAMCKP

RQARVGLLSAL*

 

>CYP81T3 JcCA0299491.10 –

71% to CYP81T1   Populus trichocarpa

complete

77% TO CYP81T3 XP_002510139.1  Ricinus communis (ORTHOLOG)
52% TO CYP81B54  JcCA0147611.20

MEVTYWSNTAALVLVLVPVLLFVLAAKFSFQKLKRSKKFPPSPPARPVIGHLHMLKQPIH

RTLHQLSEKYGDILFLRYGTRKVLLISSPSAAEECFTRNDIIFANRPRSLAGKYLNYDST

TMGFSSYGDHWRNLRRLTTIELFSTSRATMFSGIRTEEVRLLLKQLFQDSRKEPAKVALT

SKFLELTFNNIMRMIAGKRYYGKNVMDQGGEFLQDIIKEMEALRGSSNLNDYFPVLQWVD

YQGVEKRMQKLMKKMDNFLQQLIDEHMKTRGDSFPPVNLSAASNQKKHTTLIDVMLSLKE

TEPEFYTDQTIKGVILSTLTAGSQTSAATLDWAISLLLNHPEVMQKAFAEIEAVVGMERL

LDETDIPKLCYLQMIIQETLRLYPPAPLLLPHESSADCTVSGFFVPPGTMLLVNTWSMNR

NPKLWVEPTKFLPERFNGGEGEGYKFLPFGAGRRACPGAGLAKRVVSITLAALIQSFEWE

KFAKKR 

 

CYP82 family (50 sequences, 7 are complete)

 

CYP82C

 

>CYP82C24 JcCB0543141.10 +

BABX01088990.1 runs off the end at LAPY

53% to CYP82H1  Ammi majus
47% to CYP82C   JcCA0270761.10 
73% to CYP82C24 EEF43343.1  Ricinus communis (ortholog)
next best 47% to CYP82C26 XP_002510298.1  Ricinus communis 
no ESTs to extend seq, or join pieces

JcCA0116081.10 -  join with JcCB0543141.10

BABX01001818.1 runs off the end at YGMS

75% to CYP82C24 gi|223541795|gb|EEF43343.1  Ricinus communis (ortholog)

next best 62% to CYP82C28   gi|255538472|ref|XP_002510301.1  Ricinus communis

LAPYGQYWRDMRKLAVVELLSNRRLELLKHVWDTETKFFIKHLYQISVKNGGCAVVEMKD

KFNELAMNIIVKMIAGKRSFGSYGITDQDSRRFSKALADFFRLAGLLLVSDAIPFLGWLD

SLMGYVGEMKRTAREIDSALGKWVKEHRE &

KRLKGSVKGEDQDFIHVMLSVMDDGKSSIDEIDRTIKGTCL (0)

XXXXXXXXXXXXXXTWALSLLL &

SNRNVLKKAQEEIEIHVGKHHQVKESHIKNLVYLQAIVNETLRLNPAAPLSA

PREAMEDCTIAGFHIPAGTRLFVNLWKLHRDPSIWKNPSEFQPERFLNEHANLDVRGQDF

EYLPFSSGRRMCPGVSLALRVLHLTLARLLHGFELGTVSDTPVDMSESPGMTVPKAKELE

VVLRPRLPAVLYEC*

 

>CYP82C JcCA0270761.10 +

BABX01019128

60% to CYP82C2 Arabidopsis thaliana
83% to CYP82C26   gi|255538466|ref|XP_002510298.1  Ricinus communis
next best 79% to CYP82C25 XP_002510297.1  Ricinus communis

complete

MDSSLQLIAIALFFSLIFLCNGGLRGK

KSSKFSKSREAPEPSGAWPIIGHLHLLGGADKLLHQTLGL

MADKYGPAFNIRIGSHCAFVVASSELAKECFTINDKDVASRPTTAATKHMCYNHAVFGFA

PHSSHWREMRKIVMLELLSNRRLEMVKHVQNSEVNLGIKKLYHLWAENNCLPVLVELKQW

FEDLTLNVIVRVVAGKRYTGSSDDDGARRCQNAISKFFHLMGIFVVSDALPFLQWLDLEG

HQRAMKKTAKDLDAVLEGWLTEHRQKRVSVLGEAKTEGEQDFIDVMLSLEEKGHLSGFIY

DADTSIKSTCLALIAGASDTTTTTLTWAISLLLNNKSALKKAREELDLHVGPERQVDESD

LKSLVYIQAIIKETLRLYPVAPLIPREFTKDCTIAGYHVPAGTRLLVNAWKIQRDPRYWT

NPSAFQPERFLTSHANVDVRGQHHELIPFGSGRRSCPGAAFALHALHLTLARFLHAFDLA

TTMDKPVDMTETPGTTLPKATPLDVLLSPRLPAELYSC

 

>CYP82C JcCD0128885.10 + 

BABX01128855.1  runs off both ends, mid region

45% to CYP82M1v1 Nicotiana tabacum
66% to CYP82C   JcCA0270761.10
63% to CYP82C25   gi|255538464|ref|XP_002510297.1  Ricinus communis

IKQVQHTELDLGIRKLYGLWAXNSCLPLLFELNRWFDDLTLNVIVRVVAGKQYAGSSNDDESKRCRKAI

 

>CYP82C JcCB0327041.10 +  N-term

BABX01074485

79% to JcCA0270761.10
77% to x CYP82C26   gi|255538466|ref|XP_002510298.1  Ricinus communis
no ESTs to extend seq
MDSSLQLSAIVIFFSFIFLCNVYFRGTKSSKGSKGREAPEPAGGLPVIGHLHLLGGADKL 

LHQTLGTMVDKYGPVFNIRIGSRRALVVASVESAKECFTLNDKILASRPTTAATKHMCYN

HAVFGFAPFSPHWREMRKIVMLELLSNRSLEMIKQVQHTELDLGIRKL

 

>CYP82C JcCA0015251.20 – missing the N-term (off the contig)

BABX01005926

72% to JcCA0270761.10
71% to CYP82C26   gi|255538466|ref|XP_002510298.1  Ricinus communis
next best 69% to CYP82C25 XP_002510297.1  Ricinus communis
no ESTs to extend seq

SFASRPSTSATKHMCYNDAVFGFAPYSTYWREMRKIVVLGLLSNRRLEIVKN

VQASEVDKGINKLYGLWAKNTCLPLLVKINQWFDDLTLNVIVGMVAGKRFAGGSDDGEAG

WCQKTISEFFHLMGVFVISDAVPFLWWLDLQGHEKAMKNTAKYFDAILEGWVDEHRRTRA

ASGSGEFKAEGEQDFIDVMLTLEEEGQLSNFPYDSNTIIKSTILAVIAAAADTTATMLTW

AISLLMNHRWALKKAQQELDFHVGVERQVDGSDLKKLIFLQAIVKETLRVYPVAPLSGPR

EALEDCTIAGYHVKAGTRLLVNVWKIQRDPKLWTNPLSFQPERFLTSHVNVDVRGQNFEL

LPFGSGRRACPGTSFALHALHFTLARLLHSFDLATPMDQPVDMTEGTGITLPKANPVEVL

LSPRLPGKLYSS 

 

>CYP82C JcCA0015251.10 -  possible pseudogene

BABX01005926 IQRDPK exists in two different frames at the frameshift &

71% to CYP82C JcCA0270761.10

71% to CYP82C25   gi|255538464|ref|XP_002510297.1  Ricinus communis
71% to CYP82C26   gi|255538466|ref|XP_002510298.1  Ricinus communis 

MDLNLQLFAIIGFFSSIFLYSVLLRRTKITTTCNARNAPEPIGSWPIIGHLHMLLGGAYK

LLHQKLGIMTDKYGPAFCIRLGSRCAFVVASKEVAKECFTTNDRALASRPTTAATKHMCY

NNTVFGFAPYSPHWREMRKIVMLELLSNSQLEIIKNVQALVVNAGIRKLYSLWAANNNKL

PVLVELKQWSQYITFDVIVRLVAGKCYDDGEARRCQDLISEFFRLMGVFVVSDAFPFLWW

LDFQGHKRAMKKTAKDLDAVLASWLEEHRQRRISGNVKAENEQDFIDRMLSAEEKGHLSG

FPYDADTSIKSTCL

AVITGASDATATTLAWAISLLLNNPHALKKAQEELDFQVGTQRLVN

ESDIKNLAYLQAVIKETLRLYPVAPLSGPREAMEDCTIAGYHVPAGTRLILNVWKIQRDP

K &

DPKVWTKPLDFEPERFLTSHVDIDFRCQQFELIPFGSGRRSCPGVSF

ALQALHLTLGRLLQAFDLTISMDQPIDMTARSGMNTPKATPLHVLLSPRLPAKLYC*

 

>CYP82C32 JcCA0283661.10 - 

51% to CYP82Q1   Stevia rebaudiana
75% to CYP82C32 XP_002510312.1  Ricinus communis (ortholog)
59% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis
complete

MDFLAGFPTIGLVAFLLFFSCLLKILKQKISKKKNLLPPRPNGAWPVLGHLPLLRGPKQP

HVILANMADKYGPIFSIKTGVHRSLVVSSWELAKECFTTNDKAFANRPNFLAAELMGYNS

AMFGFSPYGQYWRVIRKIATVELLSNHRLQSFRHVRESEIKAAMNGIYQLWEENIDYNKA

DKGVAVKMKQWFGDITLNVIFRIIVGKRYVNYYMTSLEDGNDSDEWREAVNKFFELSGKY

VLSDSLPFLRWLDLGGYQKSMKKTAKELDVFVQSWLDEHKSKRSSSECNVKGEEDFMDVM

LSILDEAEELPCHDADTIKKATCLALTLAASDTTKITLTWALSFLLKNGEVLKKAQLELD

TEIGRERNIEEPDMKKLVYIEAIIKETLRLEPAAPLSVPHESMEDCVVGSYDIPKGTRLL

VNLWKMHRDPRIWLDPYEFRPERFLTTHKDFDVKGQNFELMPFGSGRRMCPGVSFALQVM

QLTLANLLHAFDISVPSGKQIDLVEGFGVTNNEATQLDVVLCPRLPAYLY

 

>CYP82C33a JcCB0142741.10 -   

BABX01062408.1a  runs off the conti at GDI

Contig has two sequence including JcCB0142741.20
59% to CYP82C   JcCA0055221.10
67% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis (ortholog)
next best is 63% to CYP82C34P   gi|255538498|ref|XP_002510314.1  Ricinus communis
one aa diff in overlap

MDYSLVSTNAMATTVAFLIMFCSFFWLSRSISKTAATKKKAVPKAGGAWPIIGHL

HLLGGPEPPHFVLGDMADKYGPIFTIK &

MGVHRNLVVSNWEIAKECLTTNDKAFATSPKILTTEHLANDLSMF

GFSPYGTYWREMRKIATLELLSNHRLELLRHVHKSVVKIALKELHKFWDENKISSNDKVL

VEMKRWFGDI

 

>CYP82C33b BABX01029322.1a two seqs on this contig

runs off the end at VEMK

66% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis (ortholog)
next best 55% to CYP82C32   gi|255538494|ref|XP_002510312.1  Ricinus communis

VEMKRWLGDISINLILRMIVGKSVAYMTVNQEEERWKGAMRDFFDLSGMFIAADAV

PFLRWFHIGGHEKAMNKTAKELDNVFEEWLNEHKEKKASGFKYEEEDFMDLMLNIIDDDV

QVALGRDSDTINKAACM (0)

xLTLAASDSTAVTLTWALSLLINNPDVLKKAQQELDIYVGKERLGDESDINTLVYLNAIIKE &

SLRLYPPGPLAVPHEFMEDCTVGDYHIP &

YPSGTRLITIWKIQRDPPVWSNLSD &

FQPERFLTTHEDFGVRGREYIPSSSGRRMCPGVNFALQALQLTLATLLHGLDFAS

PKNEPIDITESIGMMF*

 

>CYP82C JcCA0074591.10 + pseudogene

BABX01029322.1b two seqs on this contig  

bottom part is 65% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis
55% to CYP82C32   JcCA0283661.10
57% to CYP82C   JcCB0142741.20

MAIFYILSFPATILATILSFLLSVYYLQSRNSSSTKRERKVPEAGGAWPLIGHLHLFRGS

KPLHIILGKMADKMGPIFKIKLGMHPTLVVSNWEMAKQCLTTNDQVFATRPKTLASVLVG

YNRAMLPFSPYGPYWRQMRKIAILELLSNQRIQTQSRVRESEA

VRTFLKELYKFWDKNKNS

QNKVLVEMVRWFGDMTLNLICEIIVGKSVGYVTTEGGGESKIW
KQGLRDLFDLIGKFVAADAIPFLRWFDIGG &

IEKAMIKTAKELDLVAEGWLK

EHKKKRASGFKQGEEHFMGLMLNILDHTQVAQGPHSDPINKAVSLSLILVASDTTAVTLN

WALSLLVNNPNALKKAQDELDMHVGRERQIQESDIKNLEYLQAILKETLRLYPAGPLSLP

HESTEDCTVGGYDIPAGTKLVINIWKIHHDPRVWENPFEFRPERFLTSHKDFDVRGQNFE

FIPFGSGRRRCPGISFGLQVMQLTLANLLHSFDIETPSNEPVDMTENIGLTNLRATPLEI

LMHPRLPPHLYS*

 

>CYP82C JcCB0477511.10 + pseudogene

80% to JcCA0074591.10
53% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis

VYNILSFPATTVATVLSFLLLV &

YHQSRNSSSKKRERKMPEAGGAWPLIGHLHLLVGSKPPHMILGKMADKMGPIFK

 

>CYP82C JcCB0142741.20 –

51% to CYP82D1   Medicago sativa
62% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
62% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis

BABX01062408  bad exon boundary at GLIL

Complete

MEFLSLFSRSTVFMALAIFVPLIYSLLTKSRKARDNKIRLPLPPEPNGRLPVIGHLHLLG

GSQPPHITLEKMAEKIGPIYSIKLGVHRALIVSSWEMVKECFTTNDKAFAYRPKALFLDV

MSYDYAMFGASPYGDYWREMRKIATLELLSVRRLELLKHVRETEVKEATEGLYQGWLKSK

NSNNKLEVEMIKWFWDVSLNGILKMVVGKRYVEYIKKDEDINESGHWRYALRDFFELSGK

FAVSDALPYLRWLDIGGVEKEMQKTTKVLDNVMTGWLEEHKKKRASGMANSEEDFMDVML

SLLDDSKQISNRDADTVNKATCL (0)

GLILAASDTTKTTLTWILSLLLNNPEV

VLKKAKDELDMQVGKESKVKDSDIKHLTYLQAIIKES

LRLYPAAPLSVPRECIEYCVVGGYRIPAGTRLFVHISKIQRDPRVWENPLEFQPERFLTT

HKDIDVKGQNYELIPFSTGRRICPGASFALQVLNLSLATLLHAFDIETPLGKPVDMTEGH

GITNLKATPLDVLLTPRLPAHLY 

 

>CYP82C JcCB0388011.10 -  

BABX01078805.1 runs off the contig at VMSY

63% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
98% to CYP82   JcCB0142741.20 allele?

VMSYDYAMFGASPYGDYWREMRKIATLELLSVRRLELLKHVRE &

TEVKEATEGLYQGWLKNKNSNNKLEVEMIKWF &

WDVSLNGILKMVVGKRYVEYIKKDEDINESGHWRYALRDFFELSG

KFAVSDALPYLRWLDIGGVEKEMQKTTKVLDNVMTGWLEEHKKKRASGMANSEEDFMDVM

LSLLDDSKQISNRDADTVNKATCL (0)

ALILAASDTTKTTLTWTLSLLLNNPEVLKKAKDELDMQVGKERQVKDSDIKHLTYLQ

AIIKESLRLYPAAPLSVPRVCIEDCVVGGYRIPAGTRLFVHISKIQRDPRVWENPLEFQPERFLT

THKDIDVKGQNYELIPFSTGRRICPGASFALQVLNLSLATLLHAFDIETPLGKPVDMTEG

HGITNLKATPLDVLLTPRLPAHLY*

 

>CYP82SC JcCB0735191.10 +

BABX01101652.1 runs off at EFE

74% to JcCB0388011.10
64% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis

EFEPERFLSMNIDVKGKNPELIPFGSGRRMCPGATFALQILNLTLA &

SFLHAFEIETPSGKPVDMSEGTGIINLKATPLEVALTPRLSAHLY*

 

>CYP82C JcCB0802841.10 –

BABX01106367.1 runs off the contig end at RHT

97% to CYP82   JcCB0142741.20 
61% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
61% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
no ESTs to extend seq.

MEFLYLFSRSTVFMALAIFVPLIYSLFTKSRKARDNKIRLPLPPEPTGRLPVIGHLHLLG

GSQPPHITLEKMAEKIGPIYSIKLGVHRALIVSSWEMVKECFTTNDKAFAYRPKALFLDV

MSYDYAMFGASPYGDYWREMRKIATLELLSVRRLELLKHVRETEVKEATEGLYQGWLKNK

NSNNKLEVEMIKWFWDVSLNGILKMVVGKRYVEYIKKDEDINESGHWRYALRDFFQLSGK

FAVSDALPYLRWLDIGGVEKEMQKTTKVLDNVMTGWLEEHKKKRASGMANSEEDFMDVML

SLLDDSKQISNRDADTVNKATCLALILAASDTTKTTLTWTLSLLLNNPEVLKKAKDELDM

QVGKERQVKDSDIKHLTYLQAIIKESLRLYPAAPLSVPRECIEDCVSAATVSSRHT

 

>CYP82C JcPR03BYXIH.10 -   

BABX01043576.1 runs off both ends

78% to CYP82C  JcCB0802841.10
60% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis

VMTYNYAMFG &

SSPYGDYWREIRKIASLELLSARRLELLKHVRENEIKDAIQGLYHGWLK &

DKNSSKTKQLEVEMKQWFWDVTLNGVLKLVVGKRYVEYIKNDDDSNESGDGDWRFALRDFF

QLSGKFPVSDALPYLRWLDIGGVEKE

 

>CYP82C JcCB0894651.10 +

BABX01111516.1 runs off the contig end at HRN

61% to CYP82C   JcCA0055221.10
58% to CYP82C   JcCB0802841.10
72% to CYP82C33 XP_002510313.1  Ricinus communis
missing first 86 aa

HRNLVVSNWEI &

KSSLTTNDK

AFANRPKILAMELLGYDRSMFGFSPYGTYWRQLRKIATLELLSNHRLEMLRHVRQSEVTT

ALKELYKFWDKNKTSSNDKVLVEMKRWFGDITINLILKMIFGKSVGYMTTSHGESERWKK

ALRDFFELSGRFIAADAVPFLRWFDIGGHEKAMKETAKELDLVAEEWLKEHKKKKASGVK

HGEEDFMDVMLNTIDDDVQVALNRDSDTINKATSL (0)

 

>CYP82C JcPR03AW9Y2.10 +

BABX01041819 runs off both ends

59% to CYP82B1   Eschscholzia californica
70% to CYP82 JcCB0802841.10
64% to CYP82C33   gi|255538496|ref|XP_002510313.1  Ricinus communis

AGTINKATCL (0)

SLILAASDIVRAALTWTLSLILNNPNVLKKAKQELYTHIGQDRQVQDSDVKNLV

YLQAIVKE &

ALRLYPPGPVSVPRECIKD

 

>CYP82C JcCA0314411.20 + exon 1 plus part of exon 2

BABX01024004

46% to CYP82Q1 Stevia rebaudiana 
74% to JcCA0055221.10
71% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
71% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
no EST to extend the seq.

MNYSFPSLSSVAASVLAILTPLLIYTSFVISKMARNNKRLPPKADGGWPFIGHLHLLAGS

QPAHITLGNMAEKYGPIFTIKLGVHPTLIVSSREMAKECLTTNDKAFASRPKGLAMEILG

YNYCMVGFSPYGDYWRHMRKIITLELLSNHRLEMLKNVREAEVKMAIKGLYKEWIKNKTN

SDKILVELKTWFWDITLNVILKIIVQKRYVEEYKNGCSEGQDSSTWRDALREFMELSGKF

SVSDALPYLRWLDLGGVEKEMKENVKKIDHVLENWLQEHKQKKEDSGDLPKEEEDFMSAL

LSILDDAKELSSRDADTINKATCL (0)

TLILA &

SSDTTAITLTWTLSLLLNNRDVLKKPK

 

>CYP82C JcCB0443251.20 -  pseudogene

BABX01082246 runs off the contig at AAS, 2 frameshifts

44% to CYP82A6   Pisum sativum
72% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
72% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
90% to JcCA0055221.10
86% to CYP82   JcCA0314411.20, first 110 aa are identical

AASVLAILTPLLIYTSFVISKMARNNKRLPPKADGGWPFIGHLHLLAGSQPAHITLGNMAEKY

GPIFTIKLGVHPTLIVSSREMAK

(deletion)

ECLTTNDKAFASRPKGLAMEILGYDYSMLGFSPY

GEYWRQMRKIVTLELLSNHRLEMLKHVREAEVKMAIKALYQKWIKNKSNSNKLSAEMKRW

FWDITLNVILKIIVGKRYVEEEHTNESVEGRESGTWRDALREFMELSGKFSVSDALPYLR

WLDLGGVEKEMKENLKKLDPVVEEWXQEHKQKKEASGGVT &

KEEEDFMYVLLSILGDAKELSTGDADTINKATCM (0)

 

>CYP82C JcCA0055221.10 + exon 1, intron seq runs off the contig

BABX01027101

75% to JcCA0314411.20, nearly identical to JcCA0171811.10
73% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
73% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
96% to JcCB0443251.20
47% to CYP82D1 Medicago sativa
no EST to extend the seq.

MDVLSPFSATAMVILLAFITPIIYSLFWRSRKSCKKRLPPEAAGAWPIIGHLHLLAGSQP

AHITLGNLADKYGPIFTIKLGVYRTLVVSSRAMAKECLTTNDRAFASRPKGLAMEILGYD

YSMLGFSPYGEYWRQMRKIVTLELLSNHRLEMLKHVREAEVKMAIKALYQKWIKNKSNSN

KLSAEMKRWFWDITLNVILKIIVGKRYVEEEHTNESVEGRESGTWRDALREFMELSGKFS

VSDALPYLRWLDLGGVEKEMKENLKKLDPVVEEWLQEHKQKKEASGGVPKEEEDFMYVLL

SILGDAKELSTGDADTINKATCM (0)

 

>CYP82C JcCB0280211.10 +  

BABX01071330.1 runs off end at EDFM

79% to CYP82   JcCB0142741.20
63% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis

EDFMDVMLSLLDDSKEIYNRDAATVNKATCM (0)

AIILAASDTTKTSLTWTLSLLLNHSDVLKKVKDELDVKIGKERQVQNSDINNLTY

FNAVVKEAFRLYPAAALSVPRESNEDCVVGGYHIPAGTRLFVHISKIQRDPRVWENPLEF

QPERFLTTHKDVDVKGQHFELIPFGAGRRICPGTTLALQTLNLTLASLLHAFEIETPLGQ

PVDMTESSGITNLKATPLDVLLTPLLPAHLY*

 

>CYP82C JcCA0171811.10 +

BABX01008089 runs off the end at REFM

52% to CYP82Q1   Stevia rebaudiana
76% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis
77% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
92% to CYP82   JcCA0055221.10

MDVLSPFSATAMVILLGSRKSCKKRLPPEAAGAWPIIGHLHLLAGSQPAHITLGNLADKY

GPIFTIKLGVYRTLVVSSREMAKECLTTNDRAFASRPKGLAMEILGYDYSMLGFSPYGEY

WRQMRKIVTLELLSNHRLEMLKHVREAEVKMDITALYQKWIKNRSSSNKLSAEMKRWFWD

ITLNVILKIIVGKRYVEEEHTNESVEGRESGTWRDALREFM

 

>CYP82C JcCA0283661.20 -  C-term

BABX01020566 contig has two P450s including JcCA0283661.10

Runs off the end at LKK

97% to JcCA0105361.10
74% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis

LKKAQNELDIHVGRKRQVEESDIKSLVYLQAIIKETFRLYPAVPLLIPHESMEECIIDGN

HIPPKTRLIINAAKIQKDPIVWPNPEEFQPERFLTAHKDVDFKGQFFELIPFGSGRRICP

GISFALQVLNLTLATLLHSFEIETSSSDPIDMSETSGITNLKSTPVDVVLTPRLAANLN*

 

>CYP82C JcCA0105361.10 –

BABX01000612.1

97% to CYP82C   JcCA0283661.20
65% to CYP82C   JcCB0388011.10
74% to CYP82C29   gi|255538486|ref|XP_002510308.1  Ricinus communis

GGVPKEEEDFMYVLLSILGDAKELSTGDADTINKATCM (0)

ALILAVSDTTAITMTWALSLLLNNPDVLKKAQNELDIHVGRKRQVEES

DIKSLVYLQAIIKEAFRLYPAVPLLIPHESMEECIIDGNHIPPKTRLIINAAKIQKDPIV

WPNPEEFQPERFLTAHKDVDFKGQFFELIPFGSGRRICPGISFALQVLNLTLATLLHSFE

IETSSSDPIDMSETSGLTNQKSSPVDVVLTPRLAANLY*

 

>CYP82C JcCB0268961.10 -  

BABX01070614.1

77% to CYP82C25 gi|255538464|ref|XP_002510297.1  Ricinus communis
78% to CYP82C26   gi|255538466|ref|XP_002510298.1  Ricinus communis
no ESTs to extend seq

SDALPFLWWLDLKGHVKAMKKTAEDLDVILSGWLNKHRQRRISVPGEVKSEKDFIDMM

LSLEEKGRMSGFQYDADTSIKSTCL (0)

ALIAGAGDTITTTLTWAMSLLLNNQPSLKKAQEELDLHVGIERQVVESDIKNLVYL

QAIVKETLRLYPVAPLIPREFMEDCSIAGYRVPAGTRLLVNVGKIQRDPKFWTNPLAFQP

ERFLENHTNIDVRGHHFELIPFGSGRRSCPGASFALHTLHLTLARLLHMFDLRTSMDKPV

DMTETPGTHTPKATPLEVLLSPRLPEKLYTC*

 

>CYP82C JcCB0590781.10 – N-term

BABX01092363.1 runs off the contig at VMG

56% to CYP82C   JcCA0055221.10
58% to CYP82C   JcCB0802841.10
58% to CYP82C31   gi|255538492|ref|XP_002510311.1  Ricinus communis
no ESTs

MEFLSPLCESLDIIILSILAPVILFSFFLISRKRSANNKKRLPPEAAGGWPIVGHLPLLS

LSKAPYITLGKIADKLGPLFTIRLGVHRALIVSSWEMAKECYSTNDEAFASRPKALFFDV

MG

 

CYP82D

 

>CYP82D58b JcCB0182421.10 +

BABX01064903.1

61% to CYP82   JcCB0091131.10
65% to CYP82D1   Medicago sativa
77% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis (ortholog)
next best 53% to CYP82L7   gi|255580537|ref|XP_002531093.1  Ricinus communis 

YPPALIPG &

PRPRQFTEDCTIGGYH

VPKGTWLMLNLWKIQRDPNVWPDPLEFRPERFLTTHKNVDVRGKNFELLPFGGGRRGCPA

VSFGLQMAHLTLASLLQAFETSVPGNEPVDMSPGVGLTNMKTSHLEIVLSPRLPLSCFE* 

 

>CYP82D JcCB0091131.10 - 

51% to CYP82D1   Medicago sativa
61% to CYP82D59   gi|255538482|ref|XP_002510306.1  Ricinus communis
55% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis

complete

MEFLFVYLNAMTAGLLAPLFLFACYYVRRSSIFCSKKTRAPKAGGAWPIIGHLPLLAGSK

LLHITFGSLADRYGPIFTMQIGIHQAVVVSSGELAKELFTTNDMAVSERPNFTAFKYLGY

DGVMFAFAPYGDYWREMRKIISLKLLSNHQIELLKHVRLSEIETLLKELYKLWTEKKNGS

GHVLVDLNKWFGELGLNVILRMVVGKRCSGGGEAEHFRKTMREFFHYLGMFVLRDAIPFL

GWLDAGGHEKGMKTTAKEMNCLVSELLEEHRRKRGSAGKVATKEQDFMDSMLSVLEEKDF

AGYDSDMIIKSACLNLIAGGSDTSTVTLTWTISLLLNNKQALEKAQEELDRLVGRERQVN

EFDISKLIYLQAIIKESLRLYPPAPLSAPRVTRENCTIGGCHIKKGTRLIINVWKIHRDP

KNWPDPLQFKPERFLTSHKDIDVRGQNFELIPFGSGRRACPGTAFGLQMVHIALARFLQA

FQISNPTTEPIDMTESPGLTNLKATPLEVLLSPRLPPGLY

 

>CYP82D JcCA0263281.20 – pseudogene

missing the C-term

BABX01018294

59% to CYP82   JcCB0091131.10
69% to CYP82D57P    gi|255538474|ref|XP_002510302.1  Ricinus communis
58% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis
runs of the end at LLFC
no ESTs to extend from either end
break at GPLS is not a typical place for an intron, but the gene stops there.

LLFCYYLLRRSQSRKARAAPEAGDSWPIIGHLHLLAGRELPHKVLAALADKYGPIFTIRI

GIYPALVVSSWELAKELFTVNDPTVSSRPKLTGAKLLGYNYANFGFSPYGEYWREMRKIT

ALELLSNRKLELLKHIRASEVEGSIKDLYKFWTKDKDERNQVKVEMKRFFNDINMNVILR

MVAGKRYFGAIAGADVKVAERFQKAMRQFFQLAGVFVLRDALPFLGWLDFGGYEKSMKRT

AKEFDSIVGEWLEEHYRKRNTNGEQDFMDILLSVLDGVDLACYDADTVRKATAMTLIAGG

TDTTTVTVTWGLALLLNHPIALKKAQEEIDIQVGKERLVNEADMSKLVYLQAIVKEMMRL

YPAGPLS

 

>CYP82D JcCB0582361.10 – exon 1 only

BABX01091674.1

45% to CYP82A6   Pisum sativum
95% to JcCB0528981.10
55% to CYP82   JcCA0263281.20
64% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis

MDSLLPYLDTTMAILFALFLIFFYNNLLRKTPTPKGKPPPQPGSAWPLIGHLPLLSGSNL

PHVALATLADKYGPVLTIRIGSQPVLVLSNSEMAKELFTVNDLAVTSRPALVAAKFMGYN

YAFFPFTPAGGPCWRETRKLSTLGLLSNRRLELLKHIRIQEVEASIKELHRSLTSTNKKT

ADMKQWFSDLNHNVLLRMVIGKKYFGEGGSSGEKEGRQFQKAISVLFHYLGTLVLRDAAP

FLGWMDLGGHEKNMKKTAKVLDDILDKWLEEHKRKRYLSEEDKGEEDFMDIMLSDLDDKS

LEGFDADIVNKATCL (0)

 

>CYP82D JcCB0528981.10 - 

BABX01087902.1 runs off the contig at both ends

66% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis
94% to JcCB0582361.10
EST GW614892.1 (one aa diff) = JcCB0471441.10

MDSLLPYLDNIIAILFALFLIFFYNNLLRKTPTPKGKPPPQPGGAWPLIGHLPL LSGSNLPHVALATLADKYGPVLTIRIGSKPVLVLSNSEMAKELFTVNDLAVTSRPSLVAA KFMGYNYAFFPFTPAGGPYWRQTRKLSTLGLLSNRR

LELLKHITIQEVEASIKELHRSLTSTNKKTADIKQWFSDLNHNVLLRMVIGKKYFGEGGS

GGEKEGRQFQKAISVLFHYLGTLVLRDAAPFLGWMDLGGHEKNMKKTAKELDEILEKWLE

EHKRKRYLSEEDKGEEDFMDIMLSDVDDKSLEGFDADTVNKATCL (0)

SLIAGNETVTVALTWAMSLLLNNKQALQKALEELDIHVGRKRLVNATDIGNLVYLQAIF

KETLRLYPPAL

 

>CYP82D JcCB0501471.10 - 

BABX01086426.1b two seqs on contig

68% to CYP82D57P    gi|255538474|ref|XP_002510302.1  Ricinus communis

Runs of the end at IAGG

no ESTs to extend seq.

(0) xxIAGGTDTTTVTVTWGLALLLNHPIALKKAQEEIDIQVGKERLVNEADMSKLVYLQAIVKE

MMRLYPAGPLSGQREFTQACTIGGFHVPKGTRLILNIYKIHRDPRVWSNPKEFEPERFLN

THKDIDVKGQNFELIPFGAGRRACPGMNFGILMSQLLLASLLQAFEISAPLNAPVDMTES

AGLTNSKAAPLGVLVKPRLPAHLYE*

 

>CYP82D BABX01086426.1a two seqs on contig, pseudogene

55% to CYP82D58   gi|255538480|ref|XP_002510305.1  Ricinus communis
81% to CYP82 JcCB0091131.10

981  TIGGCHIKKGTWLIINL*KIHTDPKNWPDPLQFKPERFLTSHKDIDVRVQNFELIHLEVA

801  EELCAGTAFGLQMVHIAMARFLQAFEISNPTNEPIQKTESPGLTNLKATRL

 

CYP82J

 

>CYP82J JcCB0219051.10 –

59% to CYP82J1   Populus trichocarpa
62% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis
next best 49% to CYP82L7 XP_002531093.1  Ricinus communis

complete

MDFPSHIVAIAGVISLVLLYNLWRFGKNGTKNKGLSVPEAPGALPIIGHLHRLGGEKTLA

RTMGDLADKCGPIFTVRLGAHKAIIISNYEAMKETFTTNDILFSSRPPSSQAKYIGYNYA

AFGFAPYGTYWRNMRKLIMIELLSVQRIKLMRHVQVSEVNNMIKDLYSDSKKKIDMSEML

EHLILNIITRMVAGKRYFDGNSEGRDEHGRPIGKIMREFMFVVGALVPGDLIPFLGWWDI

QGIKKTMKKVSKELDVIMSTWIEEHKTKKNKTDSNKDFIDVMLSVIEDDTTFGHKCDVII

KSTAMSLIVAGSDTTSITMTWALSSLLNNRKTLERLQEELDQKVGRDRWVDDSDIEKLDY

FSAVIKETLRLYPPGPLGVPREATEDCTIAGYHIPKGTRLFTNLWKLHRDPNVWADPEEF

KPERFLTDNANLDVSGQHFEYLPFGAGRRSCPGLNFAMQVMNITLARLLQAYDFRTPGNE

PVDMTEGKGIALPKITPLEVVLTPRLAPELYQQLN

 

>CYP82J JcCB0246241.10 - 

68% to CYP82J1   Populus trichocarpa
75% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis
48% to CYP82L7   gi|255580537|ref|XP_002531093.1  Ricinus communis

complete

JcCB0392201.10 + join

BABX01079072.1b contig is the same as JcCB0246241.10

MDISSHLLAISVAVAVILLLYKYNQWRIRDYSHKNKGLLAPEPSGALPILGHLHLLGTEK

TLARTLARLADKYGPIFTIWLGVHRTVVVSSHEAIKECFTTNDKILASRSKSSLGKYLSY

NYAAFGFATYGPYWRDMRKIAVIQLLSNHRIKLLKHIQVSEVNTLIKDLYVLSINEPSSN

KIAISEFFEHLILNMITRMIAGKRYFNYVNDEKDEEGGKRIGKLMKEFMYVSGLFVPSDV

FPFLWWMNFLGPAKTMKRLSKELDSLLQTWIDEHKLKRINQIRDDGDCKSIEKEDFIDVM

LSVLEDDFFGHSREDIIKGTVL (0)

ALIIGGADTTSITLTWILSNLLNNRHALELAQQELDLK

VGRERCVQDSDIEKLVYLQAVIKETLRLYPPGPLAVPHEAVEDCYISRYYIPKGTRIFAN

LWKLHRDPNVWSNPDEFLPERFLANEGKNVDFSGQNFEYIPFGSGRRSCPGINFAIQAIH

MTVARLLQAFSFTTPFDQAVDMTEGIGITLPKATPLEIQITPRLSSALYEY

 

>JcCB0400141.10 +

BABX01079567

96% to CYP82J   JcCB0246241.10
78% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis
no ESTs

FLWSLREDIIKGTVL (0)

AHIIGGADTTSITLTWILSNLLNNRHALELAQQELDLKVGRERCV

QDSDIEKLVYLQAVIKETLRLYPPGPLAVPHEAVEDCYISRYYIPKGTRIFANLWKLHRD

PNVWSNPDEFLPERFLANEGKNVDFSGQNFEYIPFGSGRRSCPGINFAIQAIQMTVARLL

QAFSFTTPFGQAVDMTHEGIGITLPKATPLEIQITPRLSSELYEY*

 

>CYP82J JcCA0202251.10 +  pseudogene

BABX01011471

98% to CYP82J JcCB0246241.10 (8 aa diffs and one frameshift)
74% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis
MDISSHLLAISVAVAVILVL*KYNQWRIRDYSNKNKGLLAPEPFGALQILGHLHRLGTEK
TLARTLARLADKYGPIFTIW 
LGVHRTVVVSSH*AIKECFTTNDKI &

LASRSKSSLGKYLSYNYAAFIFATYGPYWRDMRKIAV

IQLLSNHRIKLLKHIQVSEVNTLIKDLYVLSINEPSSNKIAISEFFEHLILNMITRMIAG

KRYFNYVNDEKDEEGGKRIGKLMKEFMYVSGLFVPSDVFPFLWWMNFLGPAKTMKRLSKE

LDSLLQTWIDEHKLKRINQIRDDGDCKSIEKEDFIDVMLSVLEDDFFGHSREDIIKGTVL

ALIIGGADTTSITLTWILSNLLNNRHALELAQQELDLKVGRERCVQDSDIEKLVYLQAVI

KETLRLYPPGPLAVPHEAVEDCYISRYYIPKGTRIFANLWKLHRDPNVWSNPDEFLPERF

LANEGKNVDFSGQNFEYIPFGSGRRSCPGINFAIQAIHMTVARLLQAFSFTTPFDQAVDM

TEGIGITLPKATPLEIQITPRLSSALYEY

 

>CYP82J JcCD0129987.10 + pseudogene  N-term

BABX01129014.1

88% to CYP82J JcCB0246241.10
72% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis

VSVILVLYKYNQWRLRDHSHKNKGLLAPEPFGALPILGHLHLLGAEKTLARTLALLADKYGPIFTIWLG  &

HRTVVVS &

SHEAIKECFTANDKVL &

ASRPKSSHGKYLSYNY

 

>CYP82J JcCB0776161.10 - pseudogene N-term

BABX01104944.1 runs off the contig at YVND

69% to CYP82J5 XP_002531094.1  Ricinus communis
96% to CYP82J   JcCA0202251.10

MDISSHLLAISVAVAVILVL*KYNQWRIRDYSNKNKGLLAPEPFGALQILGHLHRLGT

EKTLARTLARLADKYGPIFTIWLGVHRTVVVSSH*AIKECFTTNDKILASRSKSSLGKYL

SYNYAAFIFATYGPYWRNM*KIAVMQLLSNHRIKLLKHIQASEVNTLIKDLYLLSINEPN

SNKIAISEFFEHLILNMIMRMIAGKRYFNYVND

 

>CYP82J JcCC0425801.10 –

BABX01123550.1 runs off contig at WID

71% to CYP82J5 XP_002531094.1  Ricinus communis
94% to JcCA0202251.10
missing the N-term   

IRDYSNKNKGLLAPEPFGALQILGHLHRLGTEKTLVR

TLARLADKYGPTFTIWLGVHRTVVVSSHEAIKECFTTNDKILASRSKSSLGKYLSYNYAA

FIFATYGPYWRHMRKIAVIQLFSNHRIKLLKHIQVSEVNTLIKDLYLLSINEPNSNKIAI

SEFFEHLILNTITRMIAGKRYFNYVNDEKDEEGGKRIGKLMKEFMYVSGLFVPSDVFPFL

WWMNFLGPAKTMKRLSKELDSLLQTWID

 

>CYP82J JcCA0254321.10 +

BABX01017296.1 runs off the ehnd at MRK

77% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis
no ESTs to extend seq

MRKLVVIQLLSSHRLKSLKHVQVSEVNTLIKD &

LYLLSLNQPSSNKIVISEFFEHLTLNMITRMIAGKRYCNCNGGNDEEGIRIGKLM

KEFMYVSGVFVPSDVIPFLWWMNFLGPVKAMKRLSKELDSLLQTWIDEHKLKGINKRRDG  

GGGGGDFKNIEEDFIDVMLSILEDNFFGHSRDDIIKGTVM (0)

TLIIGADTTSITLTWILSNLLNNRHALELAQQELDLKVGKERCVQDSDIENLVYLQA

VVKETLRLYPPGPLAVPHEAINDCYISGYYIPKGTRVLANLWKLHRDPNIWSNPDKFLPE

RFLANRDNNVDFSGQNFEYLPFGSGRRSCPGLNFAIQAIHLTLARLLQAFSLTTLFNQPV

DMTEGLGITLPKATPLEIQITPRLSSELYEC*

 

>CYP82J JcCA0250771.10 +  

BABX01016900.1 runs off the contig at DFI

67% to CYP82J   JcCB0219051.10
64% to CYP82J5   gi|255580539|ref|XP_002531094.1  Ricinus communis

DFIDVLLSEIKDDSTFGNSRKTVIKATIL (0)

TVIVGGADTTSITMTWILANLLNNK

KALELAQEEIDQKVGRDRILEESDCENLVYLKAIIKETLRLYPAGPLAVPRESMVDCNFY

GYHIPRGTRFLTNLWKLHRDPKVWANPDEFKPERFLTSHADVDVVGQNFELLPFGSGRRS

CPGLNFAMQVVQLGIGRLLQGFNLRTPSNELVDMTESLNISLSKETPLEIVLTPRLAPQL

YQAVEET*

 

>CYP82J JcCB0121261.10 +  pseudogene

BABX01061062.1

83% to CYP82J JcCA0254321.10
75% to CYP82J5 XP_002531094.1  Ricinus communis

TLIIAGADTTSIALTWIL*NLLNNRRALELAQQELDLKVGRERCVQDSDIEKLVYLQAVVKETLRL

YQPGLLAVPREAINDCY &

VSRYYIPKGTRIFANLWKLHRDPNIWSNPDEFLPERFLGNGANM &

VDFSGQNFEYLPFGSGRGSCPGINFAIEAVL &

TLTIARLFQAFNLTTTFNQPVDMTEGLGITHLPCLRLLLLEIHITPRLSSELYEC*

 

CYP82L 1 gene, 1 possible allele and 2 pseudogenes

 

>CYP82L7v1 JcCA0094171.10 – same as JcCB0076561.10 -

BABX01031541.1 exon 2

BABX01104188.1 whole gene

65% to CYP82L1   Populus trichocarpa
71% to CYP82L7 XP_002531093.1  Ricinus communis ortholog

complete

MDFSSPIQIILYSLSLLFLYALWRKITKKT TKTKKFKVPEPSGSWPLIGHLHLLTGQKPACKILASVADESGPFFSLRLGFNQILVISSW EFAKECFTKNDRVLATRASIAAGKYMGYNNAIFALAPYGQYWRDIRKLATLQLLSNNRLE ALKHVRFSEIDLFLKELYDLYSQNHNQKVIISKLLERLTFNIILRMIVGKRFCSKSYSEE NSEPWRYKKAIEEATYLFGSAVLSDAIPWLEWIDYKGYINRMKKTGKELDDLVGKWLEEH LMKKKWLNENENGENDFMDVMLSHLQEDEIANSGYSRDIVVKATAM (0)

VLSLTGAGSTAVTLTWALALLLNNPDALKSAQEELNSYVGKSKWVQESDIPNLKYLQAII

KETLRLYPPGPITGIREAMEDCDINGHYIPKGTRLIVNIWKLQRDPRVWNNPDEFLPERF

LTTHSDIDLRGQNFEFIPFSSGRRACPAINFGLQVVHLTLARLIQGFDLSRIGNVPVDMK

EGPGLSLPKVNPIEVILKPRLDLELYQCL*

 

>CYP87L7v2 JcCC0029504.10 +

BABX01123015.1 runs off at SEID

99% to CYP82L7   JcCB0076561.10 

MDFSSPIQIILYSLALLFLYALWRKITKKTTKTKKFKVPEPSGSWPLIGHLHLLTGQKPA

CKILASVADESGPFFSLRLGFNQILVISSWEFAKECFTKNDRVLATRASIAAGKYMGYNN

AIFALAPYGQYWRDIRKLATLQLLSNNRLEALKHVRFSEID

 

>CYP82L JcCB0786181.10 + pseudogene

BABX01105550.1

97% to CYP82L7   JcCB0076561.10

MDFSSPIQIILYSLALLFLYALWRKITKKTTKTKKFKVPEPSGSWPLIGHLHLL

(large deletion)

PGPITGIREA

MEDCDINGYYIPKGTRLIVNIWKLQRDPRVWNNPDEFLPERFLTTHSDIDLRGQNFEFIP

FSSGRRACPAINFGLQVVHLTLARLIQGFDLSRIANVPVDMKEGPGLSLPKVNPVEVILK

PRLRFGVISMSLVS*

 

>CYP82L BABX01079072.1b contig also contains JcCB0246241.10

pseudogene

this seq same as JcCB0392201.20
93% top CYP82L7   JcCB0076561.10

MDFSSPIQIILYSLALLFLCALWRKITKKTTKTKKFKVPEPSGSWPLIGHLHLLTGQ

KPACKILASIADESGPFYSLRLGFNRILVVSS

 

CYP83 family (5 sequences, only one is complete) probably 3 genes and one pseudogene

 

>CYP83F8 JcCA0154731.20 + 

BABX01006182.1

68% to CYP83F1v1 Populus trichocarpa
78% to CYP83F8 XP_002510500.1  Ricinus communis (ortholog)
EST GT981866 

complete

MALFIFFALALPIFLLFLLQKHKTNKNNLLHLPPGPRGLPLIGNLHQFDNSAPHRYLWQL

SQKYGPLLSLRLGFLPVLVVSSANMAKEILKTHDLIFCSRPSFRGQQKLSYDGLDLAFAP

YNTYWREMRKICIVHLFNSNRSQSFYPIRESEVSHMIDKISKLAAASKPINLSEAMMCLT

STIICRVAFGKRYEEEVGNSNTEGSRFQELLKETQALFTSFFVSDYFPFLGFIDKFTRLI

HRLEKNFREFDIFYQQIIQEHLDPNRLKPEQEDILDVLLQIWKDRSSKTDLTLDHIKALL

MNVFVGGTDTSAATVVWAMTFLMKNPLAMKKAQEEVRSVVGNKGFVNEEDTQHLSYMKAV

IKETMRLQPTVPLLIARESSQYCCLDGHDIPAKTAVYVNAWAIGRDPEVWENPEEFCPER

FVGNSIDLKGQDYELIPFGAGRRICPGMFMGLANVELSLANLLYKFDWEMPVGMKKEDLD

MEAQPGITMHKRNALSLMARKYV

 

>CYP83F9a BABX01028424 N-term

78% to CYP83F9 XP_002510498.1  Ricinus communis (ortholog)
66% to CYP83F10 EEF50805.1  Ricinus communis

MAALHISLLVLVILFPLFLLF

LVKKQTTRGLPPGPKGLPLIGNLLQLDNSNLPKHLWLLSKQYGPLMFLRLGSKPTLIVSS AKMAREVLKTQDLDFCSRPLLAGLQKLSYNGLDLAFAPYDDYWREMKKICVIYLLNSNRV QSFRPIREDEVSRMLRNISSLANDSKPVNLTEQMMALTSAAICRVAFGKRYEEGGSQANR FHQLLNETQALFVGFFFSDYFPYFGWVVDKLSGMVSRLEKNFSEFDIFYQQLIDEHLDPN REKPEYE

 

>CYP83F9b CYP83F JcCB0600081.10 -   C-term

BABX01093139.1 runs off the end at CPGI

77% to CYP83F9 gi|255538866|ref|XP_002510498.1  Ricinus communis

IKAILI (0)

NVFVAGTDTSAATVIWAMGFLMRNPEAMRKAQKEIRNTIGEKGFVNEDDVQQMP

YLKAVVKETMRLQPTVPLLVPRETVQDCTLGGFHIPAKSLVHVNAWAIGRDPEVWEKPLE

FYPDRFLNSNVDMKGQDYELIPFGGGRRICPGI

 

>CYP83F JcCA0142841.10 +  poor seq. quality at N-term

BABX01004838

67% to CYP83F   JcCA0154731.20
69% to CYP83F8 XP_002510500.1  Ricinus communis
66% to CYP83F11P XP_002535120.1  Ricinus communis

No EST to extend to N-term

HLWQLSKKYGPLM

 

MKTHNLKFSSRPPKLA

QQKLSYNGLDLAFAPYDFCYIEMKKICVVHLLNSNRVKAS

YPIREFEVTQMLENISKLADASQSIDLSKTIFSLSRTIICRVAFGKRYDEEETNQRKFQA

LLQETEALFTTFFVSDYFPFLGFIDKLIGLMHRLEKNFQEFDIFLENIVQEHLDSSRSKT

HIQEDIVDVLLQIWKDGSSKVDLSLNHIKGVIMNIFVGASDTITATVIWAMTYLMKNPIA

MKKIQEEVRGIVGNKGLVNEDDIPNLSYLKLVVKETLRMQPPIPIIPRESTQDCRIDGYQ

IPAKTVIHVNLFAIGRDPQAWENPEEFCPERFIGKFIDFKGQNCELIPFGAGRRLCPGIS

IGLATVELCLANLLYKFDWELPNGMKKEDLDMDTQPGITMHKKNALCLMARRYI

 

>CYP83F JcCA0040311.10 + pseudogene

BABX01025390.1

59% to CYP83F   JcCA0154731.20
65% to CYP83F9 XP_002510498.1  Ricinus communis

PTIVVTSAKLAQDI

MKTYDLNFCSRPPLIGSHILSYNGLDVVFPPYNDYWREMRKIGVVHLFNSNRSATFRPIR

*DEVSRLVNSVSKSAAVNKPFDLSGAMLALANNIALRAATGK

QAMFAGFFFSDYFPYVGKIVDSVTGLLPRLSKNFTEFD

LQNLFVAGTDTTTSSVVWAMCLLMKNPEKMRKAQEEIRSLKGKNGFVNEEE

 

CYP84 family (2 sequences, only one is complete)

 

>CYP84A50 JcCB0578261.10 +  exon 1

JcCB0838541.10 -   exon 2

BABX01108225

67% to CYP84A30 Vitis

76% to CYP84A1   Arabidopsis thaliana
84% to CYP84A50 XP_002533804.1 ferulate-5-hydroxylase Ricinus communis (ortholog)
next best CYP84A51   gi|255543847|ref|XP_002512986.1 ferulate-5-hydroxylase Ricinus

MDTVIHSLKDPLALALLLVIPSLLLLRLISRLRRRLPYPPGPK

GLPIIGNMLMMDQLTHRGLAKLAKQYGGLFHLRMGNIHMMAVSSPEIARQVLQVQDNIFS

NRPATIAISYLTYNRADMAFAHYGPFWRQMRKLCVMKLFSRKRAESWESVKDEVDCMIKK

VVENKGRVLNVGELIFTLTMNIIYRAAFGSKIEGKDEFIMILQEFSKLFGAFNIADFIPW

LGWIDPQGLTSRLGKARKALDRFIDLIIDQHMVKRSQGIVPDEDGDMVDDLLAFYSDEAQ

VNESDDLQNSIKLARDNIKAIIM (0)

DVMFGGTETVASAIEWALTELMKSPEDMKKVQQELAEVVGLERRVEESDF

EKLTFLKCTLKETLRIHPPIPLLLHETSADAEVAGYYIPAKSRVMINAWAIGRDKNSWED

PDTFRPSRFMKPGVPDFKGSNFEFIPFGSGRRSCPGMQLGLYALDLAVAHLLHCFTWELP

DGMKPSEIDTNDVFGLTAPRATRLIAVPNPRLLC &

Y*

 

>CYP84A51 JcCA0156071.10 – N-term

66% to JcCB0578261.10, 74% to CYP84A1 Arabidopsis thaliana

BABX01006343 runs off the contig at YRAA

BABX01103843 finishes exon 1,

81% to CYP84A51 XP_002512986.1 ferulate-5-hydroxylase Ricinus (ortholog)
next best 66% to CYP84A50 XP_002533804.1 ferulate-5-hydroxylase Ricinus

JcCB0273011.10 – C-term probable match to JcCA0156071.10 (joined)

74% to CYP84A30 Vitis
74% to CYP84A   JcCB0578261.10

MLFILLILLSLFVSIIFFLRSHMKHQYPPGPKGYPI

VGNMNIMDQLTHRGLASLAKQYGGLFHLQMGGLHVVAVSTPDMAREVLQAQDSVFSNRPA

NVAITYLTYDRADMAFANYGPFWRQMRKICIMKLFSRKRAESWASVREEVGSMIQTVMEK

TGSPVNVGEMVFALTRNITYRAAFGSFSHEGQDEFMKILQEFSKLFGAFNVADFFPWLGWIHAQKFNKRLSVA RKALDGFIDSIIDEHIEKKKKKKNDEDEDVEEDMVDELMAFYSEDVKTGFSESQSTLRLT

RDNIKALIM (0

DVMFGGTETVASAIEWAMAELMNSPQELNKLQQELTDVIGLKRILHESDLENLTYLKCVVKETLR

LHPPIPLLLHESAADS

(52 aa gap)

FIPFGSGRRSCPGLQLGLYALELTVGYLVYCFDWELPNGMKSSELDMNDLFGLTAPRAVR

LVGVPNYRLHCPF*

 

>CYP84A51 Ricinus communis XM_002512940 partial (exon 1)
XP_002512986.1

89% to JcCA0156071.10

MDSLLHLQSLQFSSMLLILLSLLISIIFLLRSRRKLPYPPGPKGYPIFGNMNIVDQLTHRGLARLAKQYG

GLFHLQMGGLHVVAVSTPEMAREVLQVQDGIFSNRPANVAITYLTYDRADMAFANYGPFWRQMRKICVMK

LFSRRRAESWASVREEVDSMVRIVMEKTGSQVNIGELVFALTRNITYRAAFGSVSDEGQDEFMKILQEFS

KLFGAFNVADFFPRMGWMHAQDFNKRLTRARQSLDGFIDKVIDEHMGKKNSSKITKDENEEETDMVDELM

AFYSEEDVAKNGIYDSQSTIKLTRDNIKAIIM (0)

DVMFGGTETVASAIEWAMAELMNSPEELNKVQQQLIEVVGLNRKVQESDLEN

LTYLNCALKETLRLHPPIPLLLHESAEDSTVAGYKIPAKSRIMINAWAIGRDPSAWADPN

KFKPSRFLEDGAPDFKGSNFEFIPFGSGRRSCPGMQLGLYGLELTVAHLLHCFKWELDEG

VKASELDMNDVFGLTAPRAVRLVAVPTYRLNCSF*

 

CYP89 family (13 sequences, 4 are complete or nearly complete)

 

>CYP89A JcCB0017561.30 +

54% to CYP89A2   Arabidopsis thaliana
64% to CYP89A98 XP_002524040.1  Ricinus communis

complete

MEIWFVIVISLSIAALLKPFLNLFFISKNSTISLPPRPLSFVGSILWLKKTSFWEFESTI

RSFHAKFGSMVALPIGTKLHFYVADGRLAHQALIQNGVSFASRPPPTNTYRIISCNQHNI

GMAFYGPTWRLLRRNLVSEMLHPSVVKSYSEARKWALQILIERLESQSKGNNSINFRHII

FCLLFVMCFGEKLDQTKIEEIERFHHSIFLNSRSARFNILNILSPRLSKIVFRKRWSELL

QLRKNLQDAFTPLVRARKKVKELGLSKENNEFMLSYVDTLLDIELPDMKRKLNEEEIVTL

CDEFFNGGTESTSTALQWIMANLVKYPHIQEKLFMEMKMVVGNEEEEHIKEEDLHKMKYL

KAVILEGLRRHPPSHMLLPHAVSDDVVLGNYLVPKNGIINFMVADIGLDPKVWEDPMEFK

PERFNGDEAFDIKGSREIKMMPFGAGRRICPGYSLGLFHLEYFVANLVWKFEWKAVDGND

IDLSEKTEVIVVMKNPLKAHLSPRFKKI

 

>CYP89A95 JcCB0156811.10 + 

59% to CYP89A2   Arabidopsis thaliana
76% to CYP89A95 XP_002520014.1  Ricinus communis (ortholog)

complete

MEVWFIIVVSLSLCFLFNSLFSLLKPAQKLPPGPTTIPIIGNLLWLLKSFSELESILRHL

RPKYGPIITLSVGSRPAIFISTHSLAHQTLIQNGVVFADRPPALPVSKIVTCNQHNVSSS

FYGPTWRLLRRNLTSEILHPSRIKSYSQARKWVLDILISRLKPCSKTGEPFLVKQHFQYS

MFCLLVLMCFGEKLEEDKIKQVERVQRRVLLSFDRFNILNFWPSLTRVLFYKRWNELLKV

KQDQESVLIPLIRARKNSKVETRSIDKEDYNKKVEKEEFILSYVDTLFDLHLPEENRKLI

ESEIASLCSEFLDAGTDTTSTALQWIMANLVKYPHIQEKLFLEINGVVGDEEVVKEDYLQ

KMSYLKAVILEGLRRHPPGHFVLPHAVTQDTVIDGFVVPKNGTVNFMVAEMGRDPKVWED

PMAFKPERFLNNGDQAFDITGSKEIKMMPFGAGRRICPGYGLAMLHLEYFVANLVWNFKW

MAVDGDDVDLSEKQEFTVVM

 

>CYP89A JcCB0883391.10 +

RUNS OFF CONTIG END

NO ESTs for the C-term

56% to CYP89A2   Arabidopsis thaliana
72% to CYP89A98   gi|255566104|ref|XP_002524040.1  Ricinus communis
72% to CYP89A100   gi|255566096|ref|XP_002524036.1  Ricinus communis

finished cannot extend

MEIWFSLIITISISALLKAIFDVFFPSNKSTTYYILPPGPVHFPIISNILWLRYSFLELT

HFIRSLHNKLGPMITVHIGSRPAIIIADRSLAHQALIQKGAIFADRPEADTIRKISSCNQ

HNINSSFYGPTWRLLRRNLTSEILHPSRVKSYSHARSWVLQILLKRLDSQSKFGKLPVRV

VDNFHFAMFCLLVLMCFGDKLDEKQIEEIERVERAAILNARKFAILNFWPRLTKILFRKR

WSDFFQLLKDREEVLLPLIRARKKVSEERQRQSNNKKEDDYVLAYVDTLLDLQLPDEKRK

LSEKEIVTLCNEFLNGGTNTTSTTLQWIMANLVKYPHIQEKLFMEIKGVVGNEEREIKEG

DLQKMPYLKAVILEGLRRHPPVHMLLPHAVSEDVILDKYLVPKNGTVNFMVADMGWDPKV

WEDPMTFKPERF

 

>CYP89A JcCB0036011.40 - 

BABX01076741.1 runs off contig end at FCLL

65% to CYP89A2   Arabidopsis thaliana
75% to CYP89A98 XP_002524040.1  Ricinus communis
75% to CYP89A100 XP_002524036.1  Ricinus communis
EST GW881640.1

JcCA0255121.10 +  N-term of JcCB0036011.40 join

METWFFVIITISISALLKAIFEVFFPRIKSASLPPGPVNFPIIGNILWLRSI AALEPIIRSLHNKLGPIITLHIGSRPAIFIADRSLAYQALIQNGAVFADRPKADAIRQVV TSNQHNISSSFYGPTWRLLRRNLTSEILHPSRVKTYSHARKWVLQILLNRFGSQSNFGKL

PLRVVDNFHFAM

FCLLVLMCFGDKLDEKQIEEIARVERASILNTRKFAILNFWPRLTKILFRKRWSEFLQLE

KDRADVLIPLIRARKRANEERQRQSNNKQEGQYMLSYVDTLLDLQLPDEKRKLSEKEIVT

LCNEFLNAGTDTTSTALQWIMANLVKYPRIQEKLFMEIKGVVGNEEEEVKEDDLQKMPYL

KAVILEGLRRHPPAHFVLPHAVSKDVILDKYLVPKNGTVNFMVADMGWDPKVWEDPMSFK

PERFVGTGETGDDVIDITGSREIKMMPFGIGRRICPGYGLAMLHLEYFVANLVWNFEWKT

VDGDDVDLTEKQEFTVVMKNPLQARIIPRCKI*

 

>CYP89A JcCD0098169.10 + pseudogene mid to I-helix

BABX01145495.1 runs off at both ends

87% to CYP89A JcCB0883391.10
96% to JcCB0036011.40
75% to CYP89A100 gi|255566096|ref|XP_002524036.1  Ricinus communis

RVVDNFHFAMF &

VFLVLMCFGDKLDEKQIEEIARVERASILNTRKFAILNFWPRLTKILFRKRWSEFLQ

LEKDRADVLIPLIRARKRANEERQ &

RQSNNKQEGQYMLSYVDTLLDLQLPDEKRKLSEKEIVTLCNEFLNGGTNTT

STTLQWIMANLVKYP

 

>CYP89A JcCB0281941.10 - 

65% to CYP89A98   gi|255566104|ref|XP_002524040.1  Ricinus communis
66% to CYP89A95   gi|255557971|ref|XP_002520014.1  Ricinus communis

complete

MELWFIILISLSISALFKSIFNLLFTSHNHRLPPGPFAFPIIGNLQWFRRSSFDIEPILR

SLHSKFGPIFTLYMGSRPAIFIADRSLAHQAFVQNGAVFADRPPALPIAKIINSNQHNIN

SASYGPTWRLFRRNLTAGVLHPSRVKSYRNAREWVLQILRNRIESQAKSGSPVSVVDHFQ

FSMFALLVLICFGDKLDETQIKQIEEVQRQILLTFGKFSSLNFWPRLSKIFMRRRWEELF

QICSKQREILIPLVRARKELKETRINKTDQQDDIIVSYVDTLLNLELPDEKRKLEEGEII

SLCSELLNGGTDTTSTALQWIMANLVKYPEIQEKLFMEIKGVISNGEEEEVKEEDLQNMP

YLKAVILEGLRRHPPGHLLLAHSVREDVVLDKYSIPKFGTINVMIAEMGWDSKVWDDPMA

FKPGRFLNDEGQLSDFDITGSREIKMMPFGVGRRICPAYSLAMLHLEYFVANLIWSFEWK

AINKEDVDLSEKLEFTVVMKNPLQAYISPRSK

 

>CYP89A JcCB0036011.30 + pseudogene

BABX01076741.1a two genes on this contig see CYP89A JcCB0036011.40

84% to CYP89A JcCB0281941.10
66% to CYP89A98   gi|255566104|ref|XP_002524040.1  Ricinus communis

MELWFIILFSLTISALFKSIFNFPFTTHNHRLPAGPIAFPIIGNLQWLRRSSFDIEPILR

YFHSKFGPIFTRHFGPRPAI &

FMAHRSLVHQAFAQNG &

AVFADRPPALPIVRIASYNQHSISS &

YGPTWRLFRRNITAVVLHPSRIKSYGNARKWVLQILRNRIKSQAKSGLPVSVVNHFQ &

KQIEEVERQILLSFGKFSVLNFWPRLSKIFMRRRWEEFFQICSKQRDILIPLIRARKELKETRINKKDEQE &

DIVLSYVDNLLDLELPDEKRKLEEGEIVSLCSELLNVGTDTTSTALQWIIANLVKYPEIQ

EKVFVEIKGVVSNGEEEVKEVDLQKMPYLKAVLLEGLRRRPPGHLLLAHSVSEDVTLDK

YLITKKGTINVM &

LAEMGWDSKVWDDPMAFNPERFLNDEGELLDFDITGSREIK

MMPFGVGRRMCPGYSLAMLHLEYFVANLIWSFEWKAINNEDIDLSEKHSPL

 

The next two probably are from one CYP89K gene

 

>CYP89K BABX01019125.1 N-term

72% to CYP89K1 XP_002539766.1  Ricinus communis
64% to CYP89K2   gi|255588234|ref|XP_002534544.1  Ricinus communis
no ESTs

MELSFFIVSLCLCLLLKPLINLLLLLHSSVSSKPINNNNNLPPGPP

RISLIANFILHRKSFFELGPIISTLRKRYGPLITVNLGSHPTIYIATNSLAHQALIQNGA

VFADRPRSINNLIVSNSKKIISKSPYGPTWRALRRNLTAGVLHPNQLKSFSNVRKRVVCN

LINNLKQDSEGNKALFVEEHFRYAVFSLLVLLCFGENVEENQIKEIEIAQQRLFKNFHRF

MLFILIPKLGKLLFRKQWKELNE

 

>CYP89K JcCB1001391.10 – C-term

BABX01059149.1 runs off at both ends

67% to CYP89A   JcCB0156811.10
68% to CYP89A2   Arabidopsis thaliana
78% to CYP89K1   gi|255616623|ref|XP_002539766.1  Ricinus communis
76% to CYP89K2   gi|255588234|ref|XP_002534544.1  Ricinus communis

TIKEDDLQKMPYLKAVVLEG &

LRRHPPGYAPATPHAVTEDAELGGYNIPKGTAVNFLIADIGRDPNVWENPMEFKPERFLK

GNFDLTGSREIKMMPFGAGRRICPGYGLAMLHMEYILANLIWYFEWKEVDGEEVDL

 

CYP92 family (2 sequences, none are complete)

 

>CYP92 JcCB0395531.10 +

BABX01079276.1

76% to CYP92A32 Vitis

74% to CYP92A62   gi|255549934|ref|XP_002516018.1 flavonoid 3-hydroxylase Ricinus
73% to CYP92A61P   gi|255604505|ref|XP_002538235.1 flavonoid 3-hydroxylase Ricinus

no ESTs to extend the seq on either end

JcPR04F0BCC.10 + C-term of a CYP92 seq (possible end for JcCB0395531.10)

BABX01048187.1

GPLPHQSLHRLSNKYGPV 

MQLKFGSFPVIVASSSEIAKQILKSNDQIFASRPQTAAGKYTTYNYSNITWAPYGPYWRQ

GRKIYLTELFSSKRLQSYEYIRVEEIRAFLARINSLSSKPIILKDHLSRVTLSIISRIVL

GKKYFSTNESENEEIVTLEEFQEMLDELFWLNGVLNIGDWIPWLNFLDLQGYVKRMKALK

IKFDRFHDHVLGEHKEKKAGEEDMVDLLLKLSNNPDLDVKLNSDSVKGFTQDLIAGGTDT

SATTVEWAMSELMKQPHLIKKATEELDRVIGKDRWVEEKDIPQLPYIDAIMKETMRKHPV

AVMLAPHLALEDTKLAGYDIAKGTRVFINTWSMGRDSSMWDEPEEFRPERFL

XXXXXXXXXXXXXXXXXXXXXXXXXXXX

GLKMIGSSLANLLHGFNWELPDNMKPEDLSMDEVYGLATPRKFPLVAIPKPRLQIHLY*

 

>CYP92A59 JcCA0307901.10 +

BABX01023275

89% to CYP92A59 XP_002521317.1 flavonoid 3-hydroxylase Ricinus (ortholog)
69% to CYP92A62 XP_002516018.1 flavonoid 3-hydroxylase Ricinus

GW613370.1

DITWSPYGPYWRQARK

 

GKKYTVKNENENEIVTPEEFKEMLDELFLLNGVLDIGDSIPWLAFLD

LQGNIKRMK

(40 aa gap)

VKLERNGVKGFTQ

DMIAGGTESSAVTVEWALSEL

LRKPEIFKKATEELDRVIGRERWVEEKDIVNLPFIEAIAKETMRLHPVAPM

LVPRQCREDTQIAGYDIQKGTRVLVNVWTIGRDPTIWDNPNEFCPERFIGKAIDVKGHDF

ELLPFGAGRRMCPGYPLGIKVIQASLANLLQGFNWKLPGNLKKEDLNMEEIFGLSTPKKF

PLVAVAEPRLPFHVYGM

 

CYP93 family (15 sequences, only 2 are complete)

 

>CYP93A52 JcCB0311271.10 +  

BABX01073598.1 runs off contig on both ends, no ESTs

67% to CYP93A53   JcCA0297371.10
84% to CYP93A52   gi|255583272|ref|XP_002532400.1  Ricinus communis (ortholog)
next best 61% to CYP93A53   gi|255583270|ref|XP_002532399.1  Ricinus communis

NKAHLPPSPRALPIIGHMHLLGPIPHQAFHRLSTRYGPLIYFFIGSRPCVLASTPEIAKE

ILKDNESNFMNRPKVANLDYLTYGSADFATIPYGPHWKFMKKICMTELLGTRTLDQFLPI

RREEIKRFLKVVFKKAEAKEAVNVGGELMRLTNNIISRMMLRTRCSDQENDADEVRKLVK

ELNELGAKFNLSDTIWFCKSLDLQGFEKRLKDARDRYDDMMERIMKEHENARKKKEKDGD

EDGNTMKDLLDILLDIYEDENAERRLTRENVKAFVMNIFGAGTDTSSITVEWGLAELINH

PDKMEKARLEIDAVVGKTRLVEESDIGNLPYLQAIVKRIL

 

>CYP93A53 JcCA0297371.10 + 

78% to CYP93A4 Populus trichocarpa
79% to CYP93A53 XP_002532399.1  Ricinus communis (ortholog)
65% to CYP93A52   gi|255583272|ref|XP_002532400.1  Ricinus communis
complete

MADFQEYIILFLIWLISVIAVRAIIKRFRSTCHLPPSPPALPIIGHLHLLAPIPHQALHK

LSTRYGPLIHLFLGSVPCVVASSPEMAKEFLKTHETSFLDRPIAAAVDYLTYGSADFSFT

PYGPYWKFMKKICMTELLGGRMLDQLLPVRHEEIGQFLKLMLKKANANEPVDVGKQLLRL

TNNVISRMLMSERCSNNEDEANEIRELVQEVAELTGKFNLSDYIWFCKNLDLQGFGRRLK

KVREKFDTMMHRIIAEHEEARKIKKETGERDSMKDLLDILLSISEDENSEIKLTKENIKA

FILDIFAAGTDTSAITTEWALAELINHPDIMKKAREEIDSVVGTSRLVEESDIINLPYLQ

SIVKETLRIHPTGPLTVRKSTEDCTINGYEIPARTQLFVNIWALGRDPKHWENPLEFCPE

RFSNAEANGKSQLDVRGQYFHLLPFGSGRRACPGTSLALQIVQSTLAAMIQCFEWKVDGG

NGTVDMEEGPGLTLPRANPLICFPMTRLNLFPSF 

 

>CYP93A54a JcCB0009781.10 –

BABX01117516.1 runs off the end at EEER, no ESTs

80% to CYP93A54   gi|255544284|ref|XP_002513204.1  Ricinus communis (ortholog)
60% to CYP93A53short XP_002532399.1  Ricinus communis

MMDIQDYTVLFLVWL

ISTILVRAIILKRRSRFHLPPSPIALPVIGHLHLLRPIPHQALDKLSKRYGSLFHLSFGS

VPCVVISSPEMAKEFLKTHEMSFCNRPSTVAVRCLTYGASDFSFAPYGPYWKFMKQICMT

ELLGGRILDRFLDIRHEELKSFLKLILRKSYEGKEVDLGEELMTFSNNVISRMTMSKRCS

TTDDEANDVRKLIEEVGVLTGEFNFQDYIWFCKNIDVQGFGKRLKRLQVKLDSMMEGILK

EHEEER

 

>CYP93A54b JcCA0029811.10 - 

BABX01022185.1

79% to CYP93A54 gi|255544284|ref|XP_002513204.1  Ricinus communis (ortholog)
66% to CYP93A4  Populus trichocarpa
63% to CYP93A   JcCA0297371.10
62% to CYP93A   JcCB0060201.10
no ESTs

EMFTTGTGTSAGVIQWAIAELINHQNIFKKAREEIDFVVGNNRLIEESDIQNLPYLQAIVK

ETLRLHPSGPLFTRESTQDCNIGGYHIPAKTRLLVNVWAIGRDPNYWENPLEFEPERFMD

NEKNGLIDV &

LLPFGSGRRSCPGTSLALQVIQTTLGCLVQCFDWKVTNDNNNVDMSEAAGISLLMANPLM
CVPTARLQYEKIIC*

 

>CYP93A JcCB0060201.10 +

82% to CYP93A53 JcCA0297371.10

74% TO CYP93A4 Populus trichocarpa
73% to CYP93A53 XP_002532399.1  Ricinus communis (ortholog)
60% to CYP93A54   gi|255544284|ref|XP_002513204.1  Ricinus communis

COMPLETE

MAYFQEFIILFLIWLASVVAVRAILKKFRATSHLPPSPPALPIIGHLHLLAPMPHQALYK

LSTRYGPLIHLFLGSVPCVVASSPEMAKEFLKTHENCFLDRPKMAAVDYLTCGSGFAFAA

YGPYWKFMKKICMKELLGGRMLDQLLPVRREEIRHFLKLMLKKASSGESVDVGPQLLMLS

NNVISRMTMSKRCSNNEDEANEVRKLLHDAAEFSGKFNLSDFIWFFENLDLQGIGRKLKK

VGEDFDKMMQRIIAEHEEARKINKEIGEGDSVKDLLDILLNISEDENSEMKLTRENIKAF

ILDIFAAGTDTSSIATEWALAELINHPDIMRKAKEEIDFVVGKSRLVEESDITNLPYLQS

IVKETLRLHPPGPLIVRQSSKDCTVNGYEIPAKTRLFVNAWALGRDPNYWENPLEFCPER

FFNTEANGNNQVDLRGLYFQLLPFGSGRRGCPGTSLALQLVQTTLAAMIQCFEWKVDGEN

GTVDMEEGPGITLPRANPLICFPVTRLNPFPSV

 

>CYP93A JcCB0742401.10 + 

1 aa dif to CYP93A JcCB0060201.10 allele

MAYFQEFIXLFLIWLASVVAVXAILKKFRATSHLPPSPPALPIIGHLHLLAPMPHQALYK

LSTRYGPLIHLFLGSVPCVVASSPEMAKEFLKTHENCFLDRPKMAAVDYLTCGSGFAFAA

YGPYWKFMKKICMKELLGGRMLDQLLPVRREEIRHFLKLMLKKANSGESVDVGPQLLMLS

NNVISRMTMSKRCSNNEDEANEVRKLLHDAAEFSGKFNLSDFIWFSIQK

 

>CYP93A JcCB0172961.10 +  pseudogene

86% to CYP93A   JcCB0060201.10
63% to CYP93A53 XP_002532399.1  Ricinus communis

GESVDVGPQLLMLSQ*M*ISRMTMSKKVLK*MKNEANEVRK &

LXHDAAEFSGKFNLSDFIWFFENLDLQGIGRKLKKVGEDFDKM

MQRIIAEHEEARKINKETGEGDSVKDLLDILLXXSEDXNSEMQLTKE &

NI*AFIL (0)

IFLAGTDTSAITIEWALAELI &

NHPDILKKVKEEIDFVVGKSRLVEESDITNLPYLQSIVKETLRIHPPGPLIVRESSKDCT

VNGYEIPAKTRLFVNVWALGRDPNYWE &

NPLEFCPERFFDTQANGNNQVDVRGQYFHQLPFGSGRRG &

PRTSLALQLVQTTLAAIIQCFKWNVDGRNSTVDTEEGPGITLPRANPLICAPVTRLNPFLSF*

 

>CYP93A JcCA0302521.10 -  pseudogene

BABX01022678.1

78% to CYP93A53   JcCA0297371.10
70% to CYP93A53   gi|255583270|ref|XP_002532399.1  Ricinus communis

MADIQDYMFHFLIWLASVIAVRAILQ*FQTTGHLPPKPPALPIIGHLHLLPPLPHQALHK

LSTRYGSLIHLSLGSVPCIVVSSLE

MAKDFLKTHEIYFLDCPKVAAVDYLTYGSAYFAFTPCGPHWKFMKKICMTELLGGRMLDQ

LFPVRREEIAQFLLTILTKANAGESFEVGRQLLRLTNNVISRITMSERCSDNEDEANEVR

ELVRDVFEVAGKFNLSDYIWFCKNVDLQGFRRRVEKVREKLNKMMQRIIAEHEEARKIKK

QRGERDLVKDLLDILLNISEDENSEMKLTK &

NKNIKAFIL (0)

DIFLAGTDTSAITIEWALAEL &

NHPDIMKKAKEELDFVAGKSRLVEDSNITNLPYLQSI

VKETLRIHPPGPLIVRESSKDCTVNGYEIPAKTRLFVNVWALGRDPNY*GNPLELCPE

RSFDTQANGNNQVDVRGQYFHLLPFGSGRRACPGTSLALQVVQTTLAAII &

QWFKWKVDGGNSTVEMEEGPGITLPRANPLICALVTRLNPFPSF

 

>CYP93A JcCA0285841.10 - pseudogene

BABX01020811.1a

67% to CYP93A53   gi|255583270|ref|XP_002532399.1  Ricinus communis
95% to JcCA0302521.10

MADIQDYMFHFLMWLASVIAVRAILQ*FQTTGHLPPKPPA

LPIIGHLRLLPPLPHQALHKLSTSYGSLIDLSLGSVPCIVVSSPEMAKDFLITHEIYFLD

RPKVAAVDYLTYGSADFAFTPCGPHWKFMKKICMTELLGGRMLDQLLPVRREEIGQFLLT

ILTKANAGESFEVGRQLLRLTNNVISRMTMSERCSDNEDEANEVRELVRDVFEVAGKFNL

SDYI*FCKNVDLQGFRRRVEKVREKFNKMMQRIIAEHEEAR

 

>CYP93A JcCA0285841.20 -  pseudogene

BABX01020811b

92% to CYP93A   JcCB0060201.10
68% to CYP93A53 XP_002532399.1  Ricinus communis

MAYFQEFIMLFLIWPASVVAVRAILKKFRATSHLPPSLPALPIIGHLHLHASMPHQALYK

LSTRYGPLIHLFLG &

VPCVVASSPEMAKAFLKTHENCFLDHPKMAAVDYLTCGSGFAFAAYGPYWKFMKKICMK

ELLGGRMLDQLLPVRREEIRHFLKLMLKKANSGESVDVGPQLLMLSNNVISRMTMSKRCS

NNEDEANEVRKLLHDATEFSGKFNLSDFIWxxxxx

DLHGIGRKLKKVCEDFE

 

>CYP93A JcCA0009062.10 +  pseudogene

BABX01031134.1b same contig as a CYP712A BABX01031134.1a

68% to CYP93A4 Populus trichocarpa
66% to CYP93A53   gi|255583270|ref|XP_002532399.1  Ricinus communis
74% to CYP93A JcCA0297371.10
87% to JcCA0302521.10

MADIQDYMFHFLIWLASVRAIPQKFQTTGHLPPRPPA &

LPIIGHLHLLPPLPHQALHKLSTCYGSLIHLSLGSVPCI

VVSSPEMAKEFLKTHEIYFLDRPKVAAVDYLTYGSGGLPHIWLS*FGLHSFGPYWKFMKK

ICMTELLGGRMLDQLFPVRREEIRQFLLTILTKANAGESFEVGRQLLRLTNNVISRMTMS

ERCSDNEDEASEVRELVRDVFEVAGKFNLSDYIWFCKNVDLQGFRRRVEKVRQKFDKMMQ

RIIAEHEEARKINKETGEGDSVKGLLDILLNRSEDENSEMKWTK &

KNIKSFIP (0)

DIFLAGT

 

>CYP93A JcCC0159991.10 - pseudogene

BABX01122598.1 RUNS OFF THE END AT LSSN

67% to CYP93A53  gi|255583270|ref|XP_002532399.1  Ricinus communis

YLLATMPHEALYMLSTRYGPLIHLFLGFVPCVVASSPEMAKEFLKTHEYCFLDRQKMAAVDYLTC

GSGFAFAAYGPYWKFMKKICLKELLGGRMLDQLLPVIRHFLKLMLKKANSG*SVDVGPQL

LMLSSN

 

>CYP93A JcCB0557381.10 - pseudogene

BABX01089972.1 runs off the end at VGR

62% to CYP93A53 gi|255583270|ref|XP_002532399.1  Ricinus communis

VGRQLLRPTNNVISRMTMSERCSDNEDEEVRELVRDVFDVVGKFNLSDYIWLCKNMDLQG

FRRRVEKVREKFDKMMQRIIAEHEEARKINKETGEGDSVKDSLDILLNISEDENSEMKLT

KENIKAFIL (0)

DIFLAGTDTSAITREWTLAEVINHPDIMKKAKEEIDFVVGKSRLAEESEITNLPYLQSI

 

>CYP93B JcCA0226911.20 + 

BABX01014236.1 runs off the end at HAT, nothing upstream for 2 kb

No ESTs

56% to CYP93B1v2   Glycyrrhiza echinata
67% to JcCA0071191.10
63% to CYP93B22 XP_002531103.1  Ricinus communis

ELTRSVVREVTQLFGQFNVSDYIWFCKNFDLQ

GFRKRFEETRRKYDSLVEGIITARQNERNDCKRNGEKYKAKDFLDIILDLMEDENTEI

KLTREHIKAMIL (0)

DFFIAGTDTTAILLEWALAELINNPKVLEKAQDEIQKVVGTHRVVEES

DCPNLPYIQAIIKETFRLHPPIPVLSRKSIEDCYVNGYKIPKNTLVFINMWAIARDSKYW

EDPFEFKPERFLSNSSNIDLKGQHFQLLPFGTGRRICPGIPLAMQELHAT

 

>CYP93B22 JcCA0071191.10 -  C-term

BABX01028942.1

77% to CYP93B22

67% to CYP93B   JcCA0226911.20
58% to CYP93A   JcCA0297371.10
53% to CYP93B1v2  Glycyrrhiza echinata
77% to CYP93B22   gi|255580558|ref|XP_002531103.1  Ricinus communis (ortholog)
next best 60% to CYP93A52   gi|255583272|ref|XP_002532400.1  Ricinus communis
no ESTs

IFFTAATDTTAITIEWAMAELINNPKVAEKAREEIDRVVGNNRIVQESD

NPKLEYIQAILKETFRLHPPIPMLSRKSIQDCKINGYTIPANSLLFVNIWSIGRDPKYWK

NPLEFTPERFLQSNSNSNEEFCQTSPVDVRGQHFQLLPFGTGRRSCPGISLAMQELPTTL

AAMIQCFDWEVVNPPEAKINGGDHVLDMTERPGLTAPRLHDLVCIPVPRLPHILDALSAT*

 

CYP98 family (only one sequence)

 

>CYP98A63

JcCA0023581.10 +

BABX01015226.1

89% to CYP98A43 Vitis, 79% to CYP98A3 Arabidopsis

BABX01102732

Cannot extend to end, no EST for the C-term

JcCB0748211.10 –

88% to CYP98A43   AM435080.1 Vitis vinifera
93% to CYP98A63   gi|255570490|ref|XP_002526203.1  Ricinus communis (ortholog)
next best 79% to CYP98A64   gi|255570488|ref|XP_002526202.1  Ricinus communis
100% to CYP98A 

MALLLISLSILFIFL 

AYKLYHHLRFKLPPGPRPWPIVGNLYDIKPVRFRCFAEWAQSYGPIISVWFGSTLNVIVS

NTELAREVLKENDQQLADRHRSRSAAKFSRDGKDLIWADYGPHYVKVRKVCTLELFTPKR

LEALRPIREDEVTAMVESIFTDCTNP (1)

 

ENNGKSLLVKKYLGAVAFNNITRLAFGKRFVNSEGIMDEQGQEFKAIVANGLKLGASLAMA

EHIPWLRWMFPLEEEAFAKHGARRDRLTRAIMEEHTLARQKSGGAKQHFVDALLTLQEKY

DLSEDTIIGLLW (0)

DMITAGMDTTAITVEWAMAELIKNPRVQQKAQEELDRVIGFERVMTEA

DFSGLPYLQCIAKEGMRLHPPTPLMLPHRANSNVKIGGYDIP

 

 

CYP701 family (only one sequence)

 

>CYP701A36 JcCA0155031.10 + 

66% to CYP701A3 Arabidopsis thaliana
79% to CYP701A36 XP_002510288.1 ent-kaurene oxidase Ricinus (ortholog)
complete
MDVITTTILPSFQAMPYATPVAVGGLFFSMFFMKKLINDQKKRNSKLPSVPEVPGLPVIG 

NLLQLKEKKPHKTFARWAETYGPIYSIRTGASTVIVINSADAAKEAMVTRYSSISTRKLS

KALSVLTQDKCMVATSDYDEFHKNAKRCILTNVLGPNAQRRHRPHRDTLVENISTRFHAH

LKSYPEQAVNFREVFESELFGVSLKEAFGEDVQSVYVDDLRTTLTREEIFEILVLDPMQG

AIDVDWRDFFPYLRWIPNTNWETKLQKMHLRRQAVMTALIEEQKKRFASGEERNCFLDFL

LSEGLLTEKQISMLVWELIIEASDTTLVSTEWAIYELAKNPKYQDLLFHEIQNVCGSEKI

KEEHLSQLPFLNAVFHETIRKYSPAPVIPLRYVHEDTEIGSYYIPAGSEIAINIYGCNMD

KKHWENPEEWKPERFLNGKYDLAELHKTMAFGAGKRACAGALQASLIGSTSIGRLVQDFE

WRLQDGEEENVDIVGLTTRKLQPLHATIKPRKTI

 

CYP703 family (only one sequence)

 

>CYP703A16 JcCB0185791.10 - 

73% to CYP703A2   Arabidopsis thaliana
86% to CYP703A16 XP_002523334.1 flavonoid 3-hydroxylase Ricinus (ortholog)

JcCB0582051.20 - 

MEFLTVSSILICIAIIANLFRWRNQKSKRLPPGPSRLPIFGNLLQLGPLPHRDLASLCNK

YGPLVYLRLGSVDAITTNDPEIIREILLRQDDIFASRPRTLAAVHLAYDCGDVALAPLGP

NWKRMRRICMEHLLTTKRLESFAKHRIDEAQHLVRYVWAQAQCGKAVNLREVLGAFSMNN

VTRMLLGKQYFGVETAGRQEAMEFMHVTHELFRLLGLIYLGDYLPFWRLIDPYGCENKMK

EVEKRVDDFHSKIIEEHKKVKNQVGEGEMDFVDVLLSLPGKDGKEHMDDVEIKALIQDMI

AAATDTSAVTNEWAMAEVIKHPRVLRKIQEELDSVVGPNRMVTESDLAHLNYLRCVVRET

FRMHPAGPFLIPHESLRATTINGYHIPAGTRIFINTHGLGRNTQVWTDVDE

FRPERHWLADGSRVEISHGADFKILPFSAGKRKCPGAPLGVTLVLMALAR

LFHAFDWAPPNGLRYEDIDTTEVYGMTMPKAQPLIASARPRLADHMYR

 

CYP706 family (7 sequences, only 2 are complete)

No sequence matches CYP706J9 in Ricinus

 

>CYP706C31 JcCA0302851.10 + 

56% to CYP706C9   Zea mays
81% to CYP706C31 XP_002509592.1  Ricinus communis (ortholog)
complete

MTPITNFLGILGTDLPTSILFTLCGIFAVIWYTWACIKSHNQRSSPLPPGPPGLPVIGNL

ASLDPELHSYFASLAHRYGPIFKLRLGYKLGIVVTSPSLVREVLKDKDITFANRDVPDVA

RSAAYGGVDIVWTPYGPEWRMLRKVCVLKMLSNTTLDSVYALRRHEVRKTVGYLYNRVGS

PVDFGEQIFLAILNVITNMLWGDTVQEEERASLGSEFRKVVADMTALLGKPNISDFFPGL

VRFDLQGLQKKMDSLSVQFDGIFERMIAKRLKMDSEGGESARECKDFLQFLLKVKEEGDT

KTPLTMTHVKALLMDMIVGGSDTSSNSIEFAMAEIMNKSEVMRKAQQELDTVVGKNNIVE

ESHIHQLPYLYAIMKESLRLHPALPLLVPHCPSETCTVGGYTIPKGARVFINVWAIHRDP

SVWEDPLEFKPERFLDSGWDYSGNDFNYFPFGSGRRICAGIAMAERMFLYSLATLLHSFD

WKLPAEGKKMDLSEKFGIVLKLKNSLIAVPTPRLSDPKLYE

 

>CYP706B JcCA0302851.20 +  pseudogene

BABX01022714 two genes also includes CYP706C31 JcCA0302851.10

56% to CYP706G1   Vitis vinifera
74% to CYP706   JcCB0049601.10
83% to CYP706B5   gi|255537053|ref|XP_002509593.1  Ricinus communis
77% to CYP706B6   gi|255537069|ref|XP_002509601.1  Ricinus communis
runs off the end at AKVI

MADSLWGWYWVPTIQRHKISEAALISISATIITLL*LSRNLKKSKIPPLPPGPRGLPVIGYLPFL

GTFLHKKFTELAQVYGPIYKLWLGNKLHVVISSPSLAKEVFRDKDKIFANRDPPMAAKVI 

 

>CYP706B JcCB0049601.10 - 

BABX01086088

55% TO CYP706G1   Vitis vinifera
67% to CYP706B5 gi|255537053|ref|XP_002509593.1  Ricinus communis
57% to CYP706B6 gi|255537069|ref|XP_002509601.1  Ricinus communis

COMPLETE

MANSLWSWYWASSSNERHNIFKAAITITVTIVTLFCLLWKLKQLKKLIPPLPPGPRGLPL

VGYLPFLGTFLHKEFTELAQLYGPIYKLWLGNKLHVVVSTPSLAKEVFRDKDKIFANRDP

PIAAKVVTYGGNEISWSSYGPEWSKMRKVFVREMLSNSNIEACYPMRKHEVEKGIRDVFK

KAGDNLSIDFGELVFEITENSAIRMLCGGMITGEKAISSLSEFRKLAGEIMVLIGRPNIF

YLFPILARFDLQGIESQARNVLSRIDEILNSIIEQRLYIDGVEEDKSKMRKKDFLQILLD

FNKNGDSETSITTDQLKAVLL

DIMVGGTDTSSTMLEWAMAEIMLHQEVMKKVYQELDEVV

GINNTVEDSHLHKLKYLDAVLKETLRLHPALPLLLPHVSSQSTTLGGYTIPKGTAVFVNV

YAIHRDPLFWDNPLEFIPERFLISESSKFDYSGNNFQYLPLGSGRRVCAGIPLAERMVMY

VLASFLHSFEWKLPQGTELELSDKFGIVVKKMKPLILVPKPRLSNVDLY

 

>CYP706B N-term

BABX01086088 second gene runs off end at TESE

65% to CYP706 JcCB0049601.10
65% to CYP706B5   gi|255537053|ref|XP_002509593.1  Ricinus communis
48% to CYP706B6   gi|255537069|ref|XP_002509601.1  Ricinus communis             
no ESTs to extend seq.

MLKTVSSFWSWCWSVFNNEEQKVSAAVLTISVATITLFCLLFLRKQKKATPPLPPG

PWGLPVVGYLPFLGTFLHKKFTELNEVYGPICKLWLGNKLCIVISSPSLAKEVVRDKDTI

FANRDPPVAAKVITYGGNDILWSSYGPEWKKIRKVFVREMLSNSNLDACCPLREREVKKA

ITYVFSRADNNSINFGEFVFEIAANAIIRMLCGGTLEGEKLGSFVPEFSRLAEEIMVLQGKPN &

CFRLFPLLARFDLQGLEKQTRKVFLFLEKILNPVIEQRLNMSAKDEKTESE

 

>CYP706B JcCB0634951.10 – possible end for BABX01086088 second gene

79% to CYP706B5   gi|255537053|ref|XP_002509593.1  Ricinus communis

DIVVGGTDTTATMLEWAMTELMLHQDVMKKVYQELDEVVGINNVVEEFHLPKLQYL

DAVLKETFRLHPALPLLAPHFSSQSCILGGYTIPKGTTVFLNVHSIHRDPLLWDNPSEFR

PERFLNSDEHIKFDYSGNNFQYLPFGSGRRVCAGIPLAERMLMYVLASLLHSFEWKLTCE

TQLELSEKFGIVIKKMNPLILIPKPRLSNLELY

 

>CYP706D JcCB0731221.10 + 

BABX01101324 runs off the end at RQQ

63% to CYP706D1   Populus trichocarpa
45% to CYP706B5   gi|255537053|ref|XP_002509593.1  Ricinus communis
45% to CYP706C31   gi|255537051|ref|XP_002509592.1  Ricinus communis
no ESTs to extend seq.

MSVCNLAKTIYNWFNSWIFNVKEQQHQIESSLFISLSVPILLACLLLWAFKNSKPKVRLP

PGPLGLPIVGYLPFIRHNMHQLFMQLAQKYGPIYKLSIGQKLCVVVSSQTLIREIVRQND

ITFANRIPSVAAKTFSYGGNDIAFAPYGPEWRVLRKIFVREMQSNANLESFYPLRRNKVR

KSVKDLFEKKSGKAINFGELIFLTIINMMSGMFWGATLDGEEEISVGAKFRSATAELVQI

LGKPNVSDLFPFLARFDIQGVEREMKEASQKIEDIYDYVIDKYVMKKELTQGKEKVEVNK

ERKDFMHFLLEFKDQETGRSISRQQXXXXXX

 

>CYP706D JcCB0949521.10 + C-term

BABX01115033

64% to CYP706 JcCB0049601.10
61% to CYP706B6   gi|255537069|ref|XP_002509601.1  Ricinus communis
runs off the contig at TVVV
73% to CYP706D1 Populus trichocarpa

TVVVWYEPTEFEPARFLDGKGSELDYNGNHLQYL

PFGSGR

RVCAGLPLAERMVMYVMATFLHMFNWELPNGEKPDTSEKF

GIVHEKATPLMVIPTPRLSNLALYA*

 

CYP712 family (8 sequences, only 3 are complete)

 

>CYP712A14 BABX01023081.1

This contig includes the gene CYP712J1 JcCA0306151.20
85% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis (ortholog)

MALGDMSFYYPLFLIWFITAFLVHLFIKSFKPPTQIHAPPSPPALPLIGHLHLIGSVLPKSFQELAGRYGPLMEIRLGA STCVVASNATVAKEIFKTHDLNFSSRPEFGSSEYFIYRGSRFVTAQYGDYWRFMKKLSMT KLLSVPQLDKFTDVCDKEKVKLVESVIRLAKEGKVCDLSKELTALTNNTICRMAMSTRCS GSNNEAEEIEELVKTCLKLTGKLSLGDVLGPLKIFDFSGTGKKLVGALTKYDRLVERIMK EHEEKAKKGLVGEKKDLLDILLEIYNDPEAEIRLSRNDIKSFLL (0)

DLFFAGTDTSSAAMQWAMGELINCPRAFNKLREEINTVVGPNR LIKESDVQNLPYLRAVIKETLRLHPSAPLIIRECAEDCKINGSIIKAKTRVLVNVFAVMR DKDSWANPGEFLPERFMESSKEKIGEHQMEFKGQNFRYLPFGSGRRGCPGASLAMLVMHA AVGALVQCFDWKVEDGDKVDLRLGSGFAAEMAKPLVCYPIKVLNI*

 

>CYP712A pseudogene? JcCC0397331.10 – N-term

BABX01123443.1 runs off the contig at KLS

70% to CYP712A1   Arabidopsis thaliana
79% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis 
40% to CYP712C   JcCA0150131.30
no ESTs
95% to BABX01023081.1

MALGDMSFYY &

IILIWFITAFLVHLFIKSFKPPTQIHAPPSPPALPLIGHLHLIGSGLPKSFQELAGRY

GPLMEIRLGASTCVVASNATVAKEIFKTHDL &

NFSSRPEFGSSEYFIYRGSRFVT

AQYGDYWRFMKKLSMTTLLSVPQLDKFTDVCDEEKVKLVESVMRLAKEGKVYDLSKEFTA

LTNNTICRMAMSTRCFGSNNEAEEIEELVKTYLKLTGKLS

 

>CYP712A JcCB0736121.10 + C-term

BABX01101718.1 runs off the end

63% to CYP712A1   Arabidopsis thaliana
41% to CYP712   JcCA0306151.20  
78% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis
87% to CYP712A14 BABX01023081.1

RRGCPGASLAKLVMHAAVGALVVLIGKLYDGDKVDLRLGSGFAAEMAKPLVCYPIKVLNI*

 

>CYP712 BABX01031134.1a

same contig as CYP93A JcCA0009062.10

93% to CYP712A14 BABX01023081.1

MALGAMSFYYPLFLIWFITAFLVHLFIKSFKPPTQIHAPPSPPALPLISHLHLIGSVLPK

SFQELAGRYGPLMEIRLGASTCVVASNATAAKEIFKT &

HDLNFSSRPEFGSSEYFI*RGSRFVTAQSGDYWRFMKKLSMTKLLSVPQLDKFTNVCD

EETVKLVESVMRLAKEGKVCDLSKEVNALTNNTICRMAMSTRCPGSNNEAEEIEELVKTC

LKHTGKLSLGDVLCLLK

 

>CYP712C3 JcCA0150131.30 +

60% to CYP712C1 Populus trichocarpa
74% to CYP712C3   gi|255584959|ref|XP_002533191.1  Ricinus communis (ortholog)
complete
MYFFNINLHIHIFIDFSMHTHYILKEKSLPFASHSKSLLVMATSSILIAWITIVAFLVRA 

LIRAITSSRTKSHQLPGPPSLPIIGHLHLLSSSLPKSLQNLASRYGPIMQIFMGGKPVVV

ISDPIIAKEILKTHDVDFASKYILGFGLSKFNMYDGCSFINSQYGPYWKFMKKLCKTQLF

AGPQLDRFIHIREQETLKLLKSLVARSGEGEPCDLGMELSNLANNVLCEMAVGKRCAENP

NLPMEIRKSIMAIMESTAKLSFTQVFGPLKKFDLSGHGKRLISVTLEYDQLLERLFKEYE

DNQMNDSGQEEKDVIDILLGTYRDTSAELRITRNQIKTFFLEIFTAGVDSTAASIQWAIA

EVINNPEILKKLREEIHSKVGSNRVVKESDIPNLPYLQAIVKETLRKYPAGPLLRRESNI

DTNLNGYDLKAGTKILINAYAIMQDPNIFKEPEKFIPERFLVDHQEMDFYGQGFHFLPFG

SGRRACIGQSHALTVTYTTIASLIQCFDWKLKDGEKFDVKLTSGYSGAMAVPLVCYPIIR

FDPFKV

 

>CYP712D JcCB0103381.10 + N-term

BABX01059960

50% to CYP712D1 Vitis vinifera
53% to CYP705B4P cottonwood 
43% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis
note: Ricinus genome does not have a CYP712D gene in current assembly

no EST to extend seq

MVSMGDIQYNWIIPLFLWLISTLLQNLFKKITQPTLHLPPSPPALLFIGHLHLLSPVVTK

CFPFLSSKYGPLLYLRLGSRPLLLVSSASFAAEIFKRNDVVFESRPKSPFVDGLLFGDYG

IMAAPYRDYWRFMKKLCVTELLGTRQVERSIGVRNEEIQRRFLKKFLEKAYRNENIDLGN

ELMKLTNNTICRMVMSTRYSEENDEAEWCRELVLGSIELVGKLAVATVLGPLKKLGFWLN

RKQLKDIPRKEDELLDKILKEHEDRAKIMVMTKRL &

DLMDILLQVCQDKYAEFKISKNHFKAFVX (0)

 

>CYP712D JcCD0051854.10 + C-term

BABX01139967

56% to CYP712D1 Vitis vinifera

no EST to extend seq

48% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis
56% to CYP712D1 Vitis vinifera
55% to CYP705B5 cottonwood

RKCDELLDKILEEHEDRAKRNNGDDKEVKDLLDILLQVCQDKDTKFKISKNHIKAFIM (0)

DLFVGGTDTSAATMQWIMAELSNHPKIFKKLRDEIKSVIGTNRLVQESDIPNLHYLQAVVTE

TLRLYPQVPIMPRECREDCEIGGFDIPKDTAVLINAYSIMRDPEIWENPNKFYPERFLQE

KEIKMQNNLNFLSFGAGRRSCPGSHLALSVIHITIASMVQCFDWKFVGDGNGGNKVNMEA

KAGMTFCLAHP

 

>CYP712D BABX01071351 pseudogene

66% to JcCD0051854.10, 
58% to CYP712D1 Vitis vinifera
48% to CYP712A14   gi|255583276|ref|XP_002532402.1  Ricinus communis
57% to CYP705B4P cottonwood

3403 DELLEKILKEHEE & 3365

3354 DGFDREDKDLMDSLLQVYKDKDAEFKISRNHMKIFFH (0) 3250

3154 ELFIAGTDTSAHTMAWAMAELMNHPNVFRKLREEIESVVGKKRLVEESDIQNLHYLQA 2981

2980 VVKETLRLYAQ & 2948

     GPAIIRQGFEDCKIGGFDIPKNTTLVINVYSIMRDPKLWENPNDFYPERFLQ 2790

2789 EKETKKQNNFSFVPFGGGRRTCPGSNLALSMVSISIASMVQCFDWKVDAGKVNWE 2625

2624 AKPGFIMYLAHPLLCVPTVHFNPL* 2553

 

>CYP712J1 JcCA0306151.20 - 

49% to CYP712B1   Arabidopsis thaliana
69% to CYP712J1   gi|255583274|ref|XP_002532401.1  Ricinus communis (ortholog)

complete

MPFIAMSIYYYAFLFLFPLLSTFLLCFLIKKHTNRNDKVRPPPSPPALPILGHLHLLGTA

FPNSFQTLARRYGPLMQLRIGFSTFVVASSAAIAKEIFKTHDLTFSSRFEMGPTDYNIYK

GTGFITGPYGAYWKFMRKLCITELFAGAQFDRFKDIREQETINLLKSINESAIEGNPCDL

NVELENFTNNIICKMTMGKKFKNINGDGEAKKMRKLVTEIMDSGAKLSGNEVFGVLKKID

IFGHGRKLKEALWEYDKVIEEIMKEYEEHLGNIGEDEEKDVMDILLMTYRDINAEVKLTR

CQIKHFILELFMASIDTESAAIQWAMAELINHPKVFKKLRDEMESTESANKRLVNESDVP

NLPYLQAIVKESLRLHTPSPIIFRESTKACKINGYDIESKTKLLINAYAIMRDPETWHDP

DKYKPERFLVKSTGEFDQFEMEMKRHNFSYLPYGGGRRMCPGSAYAYTLLYATIGALVQC

FDWKVKDGEKIDINVSTGYSGAMAPSLLCYPITHFNPF

 

CYP736 family (2 sequences, only one is complete)

 

>CYP736A JcCA0018341.10 +

BABX01009362 onr frameshift at &

55% to CYP736A17 Vitis, 55% to CYP736 XM_002531999 Ricinus communis

61% to CYP736A1 Pyrus communis
64% to XM_002511922 Ricinus communis

MLPFILAVVVVLLGSLAYFLPTLRATSSLPSEHRENDRKLPPGPSPLPIIGNLHMLGNLP

HQTLYNLAKLHGPIMSLRLGYVQTIVVSSANAAKLFLKTHDAVFGSRPKLRASRYMSYGT

KGMAFTEYGPYWRSVRKLCTVQLLSASKIEYFAPIRKEELGFYVESLKRAAAARKVVDFS

VGVGDMIQNIACRMVFGEVNNHELDLKALVKEALLLAGAFNIADYIPFLGPIDLQ (0)

GLTKRMKAFSKAMDKVL

ERIIDEHEKEAYWEKKQKSDFIDVLLSLMNQPMVTSHDNALSTIDRTHIKAISIDMIIGS

FDTSATTIEWTLSELLRHPHAMKCLQDELQSVVGMDNMVEEKHLSKLPYLDMVIKESLRL

HPIAPLLVPREAMEDVMVDGYYIPK &

SRVIVNAWAIGRDPDAWSDTEKFLPERFIDMNIDLRGQDFQLIPFGSGR

RGCPGIQLGLTIIKIIIAQLVHCFDWELPNGLLPGELDMNEKFGLSLPRANHLLVVPKYRLGT*

 

>CYP736A XM_002511922 Ricinus communis, EEF50637.1
59% to CYP736A1 Pyrus communis (pear)

MSPLMLAILIVLLASIVSFLYISKHDRKLPPGPRGFPIVGNLHK                      LGDLPHQALHHLAKKYGPIMSMRLGLVPTIIISSPQAAELFLKTYDTNFASRPNIQAS                      HYLSYGRKGLVFSEYGSYWRSTRKLCTLQLLSASKIQAFAPMRKEEYGLMVGKLKKAA                      AAREVVNLSVSVSDLIQNMSCRMVFGVSTNNGDFRLKSVVEETLRLVGAFNIGDFVPF                      LGAFDLQGVKKRSKACNEAFDKIMEKIIDEHEKEAHWENKQQRDFVDALLSVVNQSMI                      SHDGAESEIDRSNIKAILIDIIVAAVDTSATAIEWTLAELIRHPQAMKTLQDELQNVV                      GLDKMVEEKDLSKLTYLDMVIKESSRLHPVAPLLVPHESIDEITIDGYHIPKRSRILV                      NIWAIGRDSNVWSDNVDEFLPERFIGTNVDLHGHDFRLIPFGSGRRGCPGIHLGLTTV                      RMAIAQLVHCFNWKLPDGDVSPSELDMSEQFGLTVSRASHLFLVPEYRLPV

 

The next four sequences may be from the same gene

 

>CYP736 N-term

BABX01079837 runs off the end

73% to CYP736 XM_002531999 Ricinus communis

MDLTSTIVALVALAFFLQAWLRVRSTSKKNYKLPPGPRGFPIFGNLHLLGKFPHRALHQLAQKY

GPITHLRLGLVTTI

(35 aa gap)

>CYP736 JcCD0110799.10 – exon 1 fragment

BABX01126316

46% to CYP736A17 Vitis
43% to CYP71AS JcCB0793041.10
86% to XM_002531999 Ricinus communis

KNLSFASYGPYWRNVRKMCTLELLSNHKINSFKFIRK

EELDLLIKYIKEASFQREAVDLSAKVASLSADMSCRMVFGKKYMDDEFDARGFK

(27 aa gap)

>CYP736 JcCB0902881.10 – exon fragment

BABX01112214 runs off the end

44% to CYP736A1 Pyrus communis
47% to JcCD0092500.10 
41% to CYP71   JcCA0204311.10
89% to CYP736   XM_002531999 Ricinus communis

QGLTKRMKAVSKVFDGFFEKIIDEHIQFKDESRTKDFVDVMLEFVGSEVSEYHIGRDHIKAIIL (0)

>JcCB0335831.10 +  C-term

BABX01075049

83% to CYP736  XM_002531999 Ricinus communis

DMLAGSMDTSAAVIEWALSELLKHPRIMKKVQKELELKVGLNKMVEESDLENLEYLEMVIK

ETFRLHPVAPLLIPHQATQDTIVDGYLIPKNSHIVINTWAIGRDPSAWTDPERFLPERFA

GNNVDFRGRDFQLLPFGSGRRGCPGIQLGVTVVRLVLAQLVHCFDWELPNDMLPNELDMS

EEFSLVTPRAKHLLALPTYRLLV*

 

>CYP736A XM_002531999 Ricinus communis, XP_002532045.1

54% to CYP736A1 Pyrus communis (pear)
53% to CYP736A XM_002511922 Ricinus communis

MEYPHSQDMSLTWATLALIALACFLQALLWLRKTNNKKLPPGPR                      GFPIFGSLHLLGKYPHRELHRLAQKYGPIMHLRLGLVSTIVVSSPQAAESFLKTHDLA                      FASRPPHQAAKFISYEQKNLSFAPYGSYWRNVRKMCTLELLSNVKVNSFKSMRKEELD                      LLIDCIKNASCQRVAVDLSAKVASLSADMSCRMVFGKKYMDKEFDERGFKAVIQEGMH                      LGAAPNLGNYIPQIAGLDLQGLTKRMKAVSKVFDSFFEKIIDEHMEFKDENRTKDFVD                      VMLGFMGSEVSEYHIGRDNIKAIILDMLAGSMDTSAAVIEWALSELLKHPGVMKKVQK                      ELEEKVGMTRMVEESDVEKLEYLEMVIKETFRLHPVAPLLLPHEAAEDTTIDGYLIPK                      KSHIIINTFAIGRDPSAWTEAEKFLPERFLGRNIDIRGRDFQLLPFGAGRRGCPGIQL                      GMTVVRLVVAQLVHCFDWELPNGMLPTELDMTEEFSLVTPRAKHLEAIPNYRLHI

 

CYP72 clan

 

CYP72 family (4 sequences, 2 are complete)

 

>CYP72A JcCA0144411.10 + 

50% to CYP72A5 Zea mays
complete

GW616295

72% to CYP72A261 XP_002517860.1  Ricinus communis (possible ortholog)
69% to CYP72A260 XP_002517862.1  Ricinus communis

MAFLVLGLLLCVPVIVWSVKLLHKIWWTPKMIEKKLRRQGIHVLPYKLLYGNVKEMIKLA

TEAKSKPMELTHDIGHLLNPLLHQLYMTYKKPFAIWYGMTPRVTIMDPKLAKEIMTRKSE

FRKLEVTSNFKFFLRGLANIDGDKWALHRKIINPAFHTEKLKGMLPSFMASCEEMIEKWD

NLVGSKEFYELDVMPELQNLTGDIISRAAFGSNIEEGKFIFSLQRKQAGLYLQSLYSLNS

LWSRFLPSKLNKRMMQIHQQVRALLLGLVENREKAILSGNAGQDDLLNLLLKSNLDEVQE

NKNIGMSKEDVIEECKLFYFAGHETTANLLTWTMIVLSMHLNWQEKARKEIVQLLGKNKP

TFDELNRLKNVNAILLEVLRLYPATSLFRSVYEETKLAEYCFPAGVGFEIPLYLIHRDPE

LWGQDATEFNPERFSHGNSRPFFTFGWGPRVCIGQNFAMLEAKLALALILQRFSFELSPS

YKHAPNVLVTLQPQFGAQIILHKI

 

>CYP72A JcCB0881951.10 +

BABX01110460.1 runs off the end at VLSM

57% to CYP72A7   Arabidopsis thaliana
96% to CYP72   JcCA0144411.10 (3 aa diffs) possible allele or closely related gene
82% to CYP72A261 XP_002517860.1  Ricinus communis (possible ortholog)
75% to CYP72A260 XP_002517862.1  Ricinus communis
no ESTs

RFLPSKLNKR

MMQIHREVRALLLGLVESREKAILSGNAGQDDLLNLLLKSNLDEVQENKNIGMSKEDVIE

ECKLFYFAGHETTANLLTWTMIVLSM

 

>CYP72A JcCB0532981.10 + pseudogene N-term

BABX01088213.1

84% to CYP72   JcCA0144411.10
69% to CYP72A261   gi|255553639|ref|XP_002517860.1  Ricinus communis

LPYKLLYGNVKEMIKLATEAKSKPMELSHDIGHRLNP &

LLHHLYITYSK &

KPFAIWYGMTPRITIMDPKLAKEILTRKSEFRKPEISPTFKFFL

RGLANIDGDEWAQHRKIINPAFHTEKLK (0)

GMLPSFMASCEEMIEKWDNSVGSTGFYELDVQTEFQNLTGDIISRAAFGSNLEEGKLI

FALQRKQGELFLLSLFSFNSLCSRY

 

>CYP72D9 JcCA0303821.10 +

BABX01022828

67% to CYP72D1   Populus trichocarpa
75% to CYP72D9   gi|255550159|ref|XP_002516130.1  Ricinus communis (ortholog)
next best is 54% to CYP72D10   gi|255550487|ref|XP_002516294.1  Ricinus communis
N-term frameshifted, complete
MEG &

FIFPKLLLSFVLLTLYALSKVA &

KRLEKHLRKQGLKGTSYKLVYGDKKELARSSMAAMAKPIALNDPISPRVSPFFHEMVKN

YGKVSMSWFGTRPRLLLADPDLIRFVLTNKNSDFVRPPSNPLLDLLQLGVATLEGEKWTK

RRKLITPAFHFEKLKGMVPAFSTSCCDLINRWKELVSPKGYCEVDVAPEFHVLAGDVIAR

TAFGSSYQEGKKIFELQKEQVTLVLQAYMSVYFPGLRFLPTKKNKRRYSIDKEIKATLRD

MIHKKEQASSSNLDLLGLLLQYKNEPDSDLTNEDIIEECKLFYFAGQETTAHWLTWTMIV

LAMNPNWQEKAREEVLQICGKKIPEIDHLNRLKIVTMILNEVFRLYPPVPFLFRHTHRET

NIKGMLIPGGVELLLPVMFIHYDADYWGADVQEFKPERFADGVSKASKDRIAFYPFGWGP

RICLGQNFATIESKMALAMILQHFSFDLSPSYTHAPFTHITLQPQHGAPVILHHYN

 

CYP709 family (7 sequences, only one is complete) probably 4 genes

 

>CYP709F JcCA0131871.10 + 

57% to CYP709F1 Populus trichocarpa
63% to CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis (ORTHOLOG)
ONLY ONE CYP709 SEQ IN RICINUS
58% to CYP709B2 Arab.

BABX01003618

GW614777.1

complete

MGYAGFVLVALVIVVITNIWRLFKIVVWRPYVLTKSFEKQGIKGPPYKLLYGSL

AEMKELKKVARKTVLDTNSNDIIHRVVPHYHKWFSQY (1)

GETILYWHGTTPRITVTDPELAKQILSNKFGFYVKPKPTPSILKLTGQGLIFVKGGDWVRH

RRVLNPAFSVDKLKIMIKKMADCTIAVLDEWNNQCHLSDNDQRTVKIAMHENLQKLTSDV

IAHTAFGSSYKEGREAFYAQKELQKCCAASISDVFIPGTQYLPTPMNLRIWKLDKQLTNT

LKGIIESRLKTAQSSLDGCYGDDLLGIMIESLVSKTAESKNSPKLSMCEIMEDCKTFFFA

GQETTANLLTWTTFLLSLHTDWQEKLREEVLKECGMEIPDADMLAKLKLVNMVLLEALRL

YGPVIMLIRKTSEDMKLGDLMIPKDTSLTIPLVKIHRSKEYWGEDANEFNPLRFANGVSK

AAKHPNALLAFAIGPRICIGQNFAMLEAKTVLALILQRFSLSLSPEYKHAPVDYLTLHPE

YGLQVNVKSLHL

 

3 different C-terminal seqs

 

>CYP709F JcCB0250351.20 - 

BABX01069442.1

60% to CYP709F1 Populus trichocarpa
74% to CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis
runs off the end at AAED
NO ESTs

AAEDQSKPIEFNEEFEKLTSDIIAHTAFGSSYSRGKEAFEAQNELQHYCAASVLDVLVPG

SHYLPTASNRQLWKLDRKIRNSLRQIIKSRLDSASSDCNYGDDLLGLMIEASETDQNKDG

LKLNMDDIVEECKTFFFAGHETSSNVLTWTIFLLSIHQDWQTKLRQEVLEECGSKIPDSD

MLARLKLVHMVLLEVLRLYCPVVETFREATKDMKLGDLMIPKESWVCIPITELHRSKKYW

GQDANEFNPSRFANGISKAAKHPNAFIAFGMGPRTCVGQNFAMLEVKTVIAMILQRFSFS

ISPDYKHAPIDNIALQPQFGIPIIFKTSPSGNI*

 

>CYP709F JcCB0235711.10 - 

BABX01068480.1 RUNS OFF END AT RYL

59% to CYP709F1   Populus trichocarpa
64% TO CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis
81% to JcCB0551901.10
70% to CYP709F   JcCA0131871.10
NO ESTs

RYLPTPSNIRIWKLDSLLKRTLKGIIEKRLRSKDSTKTYGDDLLATMIEYSEIADETKFN

TPKLRVDEILENCKAFFFAGHETTSGLLTWTVFLLSLHQEWQERLRKEVLKECLMEIPDA

DMLAKLKLMNMVLLETMRLYGPAIDLIRTPLEDTKLGNLMIPKGTSIQIPIIRIHRNKKY

WGDDADEFNPFRFANGVSKAAKHPNAFLGFGMGPRVCIGQNFAMLEAKTVLTLILQRLSL

SLSPEYKHTPVNHITLHPHNGLPIIVEPLSL*

 

>CYP709F JcCB0551901.10 + 

BABX01089677.1

59% to CYP709F1   Populus trichocarpa
65% to CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis
runs off at TYD, NO ESTs

TYDIIAHTAFGSSYGQGKEAF

NAQKELQKCCVAANSAFFIPGSQYLPTPSNIRIWKLDSLMKSTLRGIIEKRLRSKDSTKN

YGDDLLGTMINGYSEIAGETKLSVNEILENCKAFFFAGHETTSSLLTWTIFMLSLHREWQ

ERLRIEVLKECSMEIPDADMLAKLKWMNMVLLETLRLYSPIINLLRVLVKDTKLGNLMIP

KGTCIQIPVTKVHRSKEYWGEDADAFNPFRFGNGVSKAAKHPNAFIGFGMGPRVCIGQSF

AMVEAKIVLALILQRFSLSLSPEYKHTPINHITLHPQYGLPIIVKPLS*

 

3 different N-terminal seqs

 

>JcCB0445171.10 – N-term

BABX01082353.1

50% to CYP709B1 Arabidopsis thaliana
80% to JcCB0827551.10
55% to CYP709   JcCA0131871.10 
49% to CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis
runs off end at EFIK, NO ESTs

MSYLVAFVVGLVVILVTKIWKVLWKPYALTKRFEKQGIKGPPYRFIYGSIEEMKKLVIAA

ADIVLDTNSNDINQKVIPYYHKWSSQY (1)

GETMYFWYGMEARMTITDPELIKEVLSNKYGFY

VRSSVRPTLKTLVGKQSLIFVEGHDWLRQRKLLNPAFSIDKLK (0)

IMTKKMADCTIAMLEGWKNQPLEAYKETQCMKIEMSQEFIK

 

>JcCB0397941.10 - N-term

BABX01079429

41% to CYP709B1   Arabidopsis thaliana
60% to CYP709   JcCA0131871.10
66% to CYP709F3 XP_002515162.1  Ricinus communis
no ESTs

MGYDVGIFMVALTVLVVSKIWRLVKISIWKPYALTRSFRRQGVSGPPYYLFSGSLNDWKR

LKMDATQTVLDTNSNDITPRVLPHYHKWCQQY (1)

 

>JcCB0827551.10 - N-term

BABX01107597.1 runs off the end at FEK

56% to CYP709F3   gi|255548211|ref|XP_002515162.1  Ricinus communis

ESTs GW617334.1, GW878208.1

MGYFVAFVVAVVVILVTNIWRLCWILLWRPYVLTKT

FEKQGIKGPPYKFIYGSIKDMKKLGVAASDIVLDTNSNDIIQKVTPYYHKWSSEY (1)

GETMLYWYGTEARITITDPELIKQVLSNKFGFYVRPWVRPTIKTL

MGQKGLIFVEGHNWVRQRRLLNPAFSIDKLK (0)

TMTKKMAECTIAMLEGWKKAYKNKKCMKIEMSQEFIKLTSDIIAHTAFGSSYVQGKEAFNAQ

 

 

CYP714 family (9 sequences, only 2 are complete)

 

>CYP714A20 GW616570, GW619672
BABX01093907.1
54% TO CYP714A1 Arabidopsis thaliana
83% to CYP714A20   gi|255550874|ref|XP_002516485.1  Ricinus communis (ortholog)
67% to CYP714A21   gi|255548610|ref|XP_002515361.1  Ricinus communis

JcCB0557511.10 – exon 2

BABX01089980.1

GPIYIYSTGFKQHLYVTDPEFVKEMNQCNTLALGKPPYVTKRFAPMFGN

GIMRSNGHFWALQRKIVAPEFFMGKIK (0)

SMVGLMVESTQVLIGEWEQRIGTQGGVPAEIRV

DEDLRGLSANVIARACFGTSYFKGKQIFSKLRFLHKALTRQGTLFGSTNFR ()

KNCREISSLEREIETLIWETVQERQRQSSIEKDLMQLILEEAVNNTNAGKLSPKQFIVDNCKSLYFAGH

DTTAISATWCLMLLALHPDWQ

SKIRAEITQFCNSELLDANSLSNLKT (0)

VTMVIQESLRLFPPAGFV

 

>CYP714A20 JcCB0609731.10 -   

54% to CYP714A1   Arabidopsis thaliana
97% to CYP714 GW616570, GW619672 only differs at end 5 diffs in 17 aa 
(seq error?)
83% to CYP714A20   gi|255550874|ref|XP_002516485.1  Ricinus communis

HFWALQRKIVAPEFFMGKIKSMVGLMVESTQVLIGEWEQRIGTQGGVPAEIR

VDEDLRGLSANVIARACFGTSYFKGKQIFSKLRFLHKALTRQGTLFGSTNFRKNCREISS

LEREIETLIWETVQERQRQSSIEKDLMQLILEEAVNNTNAGKLSPKQFIVDNCKSLYFAG

HDTTAISATWCLMLLALHPDWQSKIRAEITQFCNSELLDANSLSNLKT

VTMVIQESLRLFPPAGF

 

>CYP714A21 JcCA0079341.30 –

BABX01029869.1

59% to CYP714A1 Arabidopsis thaliana

77% to CYP714A21   gi|255548610|ref|XP_002515361.1  Ricinus communis (ortholog)
64% to CYP714A20   gi|255550874|ref|XP_002516485.1  Ricinus communis

MEVSVLLEIVCWLFISGLVWFIIHLYKTTLLRSQKVKRKLRVQGIKGPTPSFLCGNLSEM

QQIQSMAISSRNHANIVAHDYASSIFPYFEHWRKQY (1)

GLIYTYSTGLKQHLYINDPEMVKE

MNQSISLDLGKPTYMTKRLQPMLGNGVIRSNGHLWAQQRKIIAPEFFKDKVK (0)

GMVGLMVESAQPMLRKWEEFVEAEDGSPVDIRVDEDLRELSANVIARACFGSSYSQGKQI

FSKLRTLQQLLSNESSPFSVTDFG (2)

FFASKKRKRLSSLEKEIDSLIWDT

VKEREKQCSEKSYI

EKDLMHRLLEESVNDSNSSKFSAKSFIVDNCKNMYFAGHESTATAASWCLMLLALYPEWQ

SRIRQEVNQFCSDGLDKNSISNLKT (0)

LTMLIQETLRLYPPAAFVSREALEQVQIGKISIPK

GVCIWTLIPTLHRDPILWGPDVNEFRPERFADGISKACKFGHAYIPFGVGTRLCLGRNFA

MIQLKIVLSLIVSKFTFSLSPNYQHSPAFRMLVEPQHGIQIVIQKV*

 

>JcCA0108741.10 – exon 1

BABX01000990.1

58% to CYP714 JcCA0079341.30
63% to CYP714A20   gi|255550874|ref|XP_002516485.1  Ricinus communis

MEISLLRIFSSVAIIGVVYLIKEILLGPKKAKKKLQMQGIKGPSPSFLYGNLPEMQEIQF

KNSRKEAPTQVDDIVAQDYTSIIFPYFEHWRKQY (1)

 

>BABX01140793.1 JcCD0059111

83% to Ricinus communis XM_002516439

74% to CYP714A   JcCA0079341.30

SLRLFPPAGFVVREAFQEVQIGNITIPKGVCTWTLISTLHRDPNIWGQDADKFRPERFAD

GLSKACKFPQAYIPFGIGTRLCVGRHFAMVELKIVISLIISKFVLSLSPNYMHSPAFRML

VEPQHGLQLIIQKI*

 

>CYP714A20 Ricinus communis XM_002516439, XP_002516485.1

83% to GW616570, GW619672

MEIYSLLLSAFGVIGFVYCFIHIFKESLLGPFAVKRKLHIQGIK              

GPSPSFLYGNLSQMQQIQLKTKKEPPPHDQIAALDYTSIVFPYFEHWRKQYGPVYTYS

TGFKQHLYLTDPELVREMNQCNTLDLGKPPYVTKRFAPMFGNGIIRSNGHFWALQRKM

VAPEFFMFKVKSMVGLMVESTKPLIREWEQRIETQGGAQAEITVDDDLKGLSANVIAR

ACFGSSYFKGNEIFSKLRTLHKALTKHATLFGFSGFRKNYREIRSLEREIESLIWDTV

QERQRQGLTERDLMQLILEEAVNNTDAGKLSPKQFIVDNCKSLYFAGHDTTAISASWC

LMLLALHPDWQSQIRAEVAEFCNDELLDANSLSNLKTVTMVIQEALRLFPPAGFVVRE

AFEEVKIRNIIIPKGVCTWTLISTLHRDPSIWGPDADKFNPGRFADGISKACKFPQAY

IPFGLGTRLCVGRNFAIVELKIVISLIVSKFRISLSPNYVHSPVLRMLVEPEHGLQLL

IQKI

 

>CYP714E16 JcCB0338361.10 +  exon 1

58% to CYP714E4 Populus, probable N-term to JcCA0300951.10 (ortholog)

76% to CYP714E16 XP_002526382.1  Ricinus communis

JcCA0300951.10 –

BABX01022503 C-term part

73% to CYP714E4 Populus trichocarpa 
finished, cannot find exon 2

MELEFGFKILISVAVIGFFGLVVLLYDGLVVKPQRLRSKLKKQGVDGPKPSFLLGNLREI

RKAQASVLKVQQTPIIHNTAAQIFPFYEQWKNQY (1)

(missing exon 2)

GMVKLINESTKTMINSWKEEIEAKGGIADIKIDEGLRSFSGDVISRACFGSNYTKGEKIFL

KLRALQKVMSKKALGIIPGMRYLPTKHNREAWALEKGIRNLIIEVVTERQKATDKNNDLL

QMVLEGAKTSKLKGEAMNRFIVDNCKNIYLAGFETTAVAATWTLMLLAANQDWQDRVRAE

VLEICGGGMPDYEMVRKMKLLNMVIHESLRLYPPVPIISREAFQDMKFGNINAPKGVNVW

TMVTPLHTDPEIWGTDSYNFNPQRFANGIAGACKFPYSYMPFGVGPRVCLGQNLALVELK

ILIALILCNFSFSLSPKYIHSPVIRLVIEPENGVNLLIKKL

 

>CYP714M

 JcCB0058861.10 pseudogene upstream of JcCB0058861.10 – N-term seq

65% to CYP714M3P   gi|255582040|ref|XP_002531817.1  Ricinus communis

2890  GPVYMYSTINNQHLHVDHPKVMKAANLHKSLD  2795 &

2793  KPTYLSKAVEPLIGNRIIRANGP  2725 &

2720  WAHKMKLNAPELFLNKAK  (0) 2667

1861  HMLGVMEESTIAMIK  1817

 

>CYP714M JcCA0282821.10 +

BABX01020472.1

66% to CYP714M1   gi|255582038|ref|XP_002531816.1b  Ricinus communis
48% to JcCA0300951.10
46% to CYP714A JcCA0079341.30
54% to CYP714H1 soybean
73% to Ricinus communis XM_002531771

GW617797.1

CYP714 JcCB0058861.10 – probable N-term for this seq.

BABX01092189.1

complete 

GT973681

MAAGFAVAADVFWSAIIVGICILLTRIWHVMWLKPRRIRSMLLKQGIRGPKPRFFYGNTQ

EMKNIQSNISNTHLQPRPIDAISHSAWVRSMLPYFQQWAQQY

GPVYMYSTMNKQHLYVDHPELIKAMNLHNSLDLGRPRYLSKPMAPLFGNGIIRANGPHWA

HQRKLIAPEFFLNKAK

HMLEVMEESTIAMIKKWQTKIENNGRVADVSVEKELKGVAADIISKVCFGSSYFQGKQIFA

KSAALQEALSKPSSLFGLSNFRFLPTKSNREIWRLQKEVETMILQIVNERREKSKGSNKP

EEDLLQAILENATNRDTHLQATANINSIIVDNCKNIYFAGHETTALTASWCLLLLALHPE

WQERVRAEIAEICGDNLNDCLLDLDKLRQLKTLNMVIQETLRLYGPAVIAAREAFADIKL

GDMTIPKGTNIWILITALHRDPENWGPDANEFKPERFAGGIAEACKYPQSYIPFGFGNRL

CIGQTFAYLQLKILLSLILSNFCFALSPEYCHSPKYNMLLVPEHGIRLLVSKVNGY

 

>CYP714M3P Ricinus communis XM_002531771 partial seq

XP_002531817.1

MEGIVGAVVAVVLCIVFIRVVDEMWRKPRRIRSMLLKQGIRGPTPSFLYGNIQDME

 NLAPPIA        IIPNCH SW

YGPIFMYSTANKQHSYVGKPELMKALNLH

KSLDLGRPNYLSKTTGPLLGNGIISANGPHWAQQRKLIAPEFFLHKVK (0)

HMLGLMEESTMAMISNWRSQVERMGGVADINVDKDLKSASADIIS              

KACFGSSYSQGKQIFAKLDALQGAMSNTSILFGFPNFRFLPTKSNREIWSLQKEIETL 

ILEVVNARREERQRSRKSENDFLEAILESAADIEELEHTHNTDRFIVDNCKNIYFAGQ 

ETTALSASWTLLLLSLHPESQDRVRAEIVEICGDRIQDSLLDLDKLRQLKTLNMVIQE 

SLRLYGPAVIAGREAFDDMKMADLTVPKGTYIWVLIPALHRDPENWGPDANEFKPERF 

AGGTIEACKHPQSYIPFGLGSRVCLGQTFAMLELKILLSLILSDFSFSLSPLSIECY

 

>CYP714M JcCA0144051.10 -  pseudogene EXXR motif missing R

BABX01004973.1

74% to JcCA0282821.10, 70% to Ricinus communis XM_002531771
69% to CYP714M3P   gi|255582040|ref|XP_002531817.1  Ricinus communis

NMMGLMEESTIAMIRRWQRQVESNGGVVDIGVDKDLKSVAAVINSKACFSSSYSQGKQIF

SKLRALQAAISQPNLLFGLTNFR (2)

ILSTQNNRQIWKLQKDVET &

LILDIVNVRRQERGRSNKYEEDLLQVIPEI

AENSEDLQHISSKDRFIIDNCKNIYFAGHETTALTASWCLLLLALHPEWQERVRAEIAEI

CGDNLNDCLLDLDKLRQSKT (0)

LNMVIQETLLLYGPAVIAAREAFADIKLGDMTIPKGTNIW

ILITALHRDPENWGPDANEFKPDRFAQGIIEACKFPQLYIPFGFGSRLCFGQTFAML

*IQLLVSLMISSFSFSISPKYRHSPLYKMLLVPEHGIRLLVRKS*

 

 

CYP715 family (3 sequences, none are complete)

There are probably 2 genes. The top two could join

The last two look different from both of the top two

 

>CYP715 BABX01020427.1 N-term

JcCA0282411.10 + /pseudo

77% to CYP715A19   gi|255545268|ref|XP_002513695.1  Ricinus communis

no ESTs to extend the seq.

LIFKVFNLTIVSIFICLLFKVLI

SFWISPVMTYMKLKRNGFGGPTPKFPLGNIKEIKKISTTTNSTSSSGFLNFSNDIHSTVF

PYFAQWQKSH (1)

GKVFIYWLGTEPFLYIAEPEFLKTMSTGVMGKSW

GKPDVFKHDRKPMFGNGLVMVEGDDWVRHRHVITPALSPANLK (0)

 

>CYP715 JcCA0073861.10 +

BABX01029242.1 runs off the end

76% to CYP715A6 Vitis

85% to CYP715A19   gi|255545268|ref|XP_002513695.1  Ricinus communis

no ESTs to extend the seq.

Probable end for the BABX01020427 sequence

EKLRAMQYTLFKTNRYVGVPFSKFFCPKQTLEAKNLGKEIDDLLLSIIADRKKSSNGGYL

QKNLLGLLLEENHEDGRLGKTLTSRELVDECKTFFFGGHETTALALSWTLLLLAIHPEWQ

NRLREEIKEVIGDKEIDFSMLSGLKK (0)

MGWVWNEVLRLYSPAPNVQRQTREDITVNKLTIP

KGTNVWIDVVGMHHDPNLWGEDVYEFKPERFKEDLYGGCKHKMGFLPFGFGGRMCVGRNL

AMMEYRVVLSLMLTRFSFSVSPTYSHSPSIMLSLRPSLGLPLIIQPLCD*

 

>CYP715 JcCA0213041.10 +

BABX01012691.1

85% to JcCA0073861.10

90% to BABX01020427.1 N-term (4 aa diffs)
82% to CYP715A19 XP_002513695.1 Ricinus communis

GKPDVFKNDRKPMFGNGLNMAEGDDWVRHRHFITPALSPANLK (0)

AMTSLMVESTTNMLDKWGALVNSGYKEIDVEREIIAIAGEIIAKTSFGIGYEDGREVI

EKLRAMQYTLFKTNRYVGVPFSKLLCPKQSLKAKSLGKEIDDLLLSIIRDRKKSKNGGKLQKD

LLGFLLGENHEDGQLGKTLTTRELVDECKTFFFGGYETTALALSWSLLLLAMHPQWQNRL

RDEIKQIIGDKEIDFSMLAGLKK (0)

 

BABX01043978.1 runs off the end

Probable end for the BABX01012691.1 sequence

PFGFGGRMCVGRNLAMMEYKVVLSLILTRFS &

FSVSPTYSHSPSIMLSLRPSLGLPLVIHPLCN*

 

 

CYP721 family (4 sequences, only 1 is complete) probably 3 genes

 

>CYP721A30 JcCB0108161.20 –

BABX01060279.1c

53% to CYP721A1   Arabidopsis thaliana
77% to CYP721A30   gi|255538580|ref|XP_002510355.1  Ricinus communis (ortholog)

complete

MLLFHSIVLRILIFLLLIYILKLIHSIVWDPLRIKIHFKNQGINGPSYRPIVGNSAEIRR

MYAEEQTKSTSTLSISHDILHRVVPFYHHWSRMYGKTFLYWFGSRPRLVISNPDSIKEVL

MNSGNGVFEKVPQNPQAKLLFGDGLVQLHGEKWALHRRISNQAFSMERVKGWVPEIVEST

TKMLKKWEEIRGGRDEFEIEVNKELHELSADVISRTAFGSSFEEGKRIFALQEQQMHLAS

QAIRSVYIPGFRFLPTKKNRERWRLDKETRESIRTLIKTSSSARQNSRNLLSLLLSSYKN

NQNGKEEKLTEEEIIDECKTFYFAGKETTANLLTWALVLLAVHQEWQDKARQEVFQICGG

GNEFPVAENLNNLKIVNLIINETLRLYPPAVSLTRQATKRVKLGRLDIPGGTELYLPLTA

IHHDTDIWGEDASEFNPSRFNESKRHLAWFFPFGLGPRICAGQNLAIVEAKIILAIIIRH

FSFVLPPTYVHAPMLLVTLMPQYGAQIIFSRISS

 

>CYP721A BABX01060279.1b

57% to CYP721A30   gi|255538580|ref|XP_002510355.1  Ricinus communis
63% to CYP721A2v1 cottonwood
58% to CYP721A30   JcCB0108161.20
66% to CYP721A JcCD0092113.10

MFAAILFFLLILLFLLKFAHSILWVPNKTEPQFRKQGIRGPGYRPMFG &

NLAEMRRMTAEARSIRMALNHNILSRVSPFYEKWSNEYGEPL

LYWSGTRPRLAIAESGLIKEILVDTSGVFVKPDIDPLSKQLLGDGLAALNGEKWTVHRR

ITNVALNMEQVK (0)

GWIPKIVASTVKMLDNWEEKRKGKDEFEMGVHRELHNLSAEIISRTLFGSSFEDGKHIF

KLQEKQMQLVLQALRTVYIPGLK (2)

FLPSKMNRERWMLEKEIHGLIRNLIEINGKERENSRNLLSLLTSSYKNRDGKDEKLD

IEEVIDECKTFYFAGKETSANTLTWTLILLAMHQEWQIQAREEVVRVCRDKELPTADVL

GELKI (0)

LNMILNDTLRRYTPVVGLVRQATKDVKLGHTEIPANTQLALAINKAHRDTRIWGSDPD

KFNPETFREARKHSALFFPWSLGPRICVGQSLAMAEMKN (1)

SIIIRHFSFVVSPTYVHAPINFLTVLPQYGAQILFRKI*

 

>JcCB0108161.10 -  exon 1

BABX01060279.1a three genes on this contig including CYP721A30

44% to CYP721A1   Arabidopsis thaliana
53% to CYP721   JcCB0108161.20
48% to CYP721A30   gi|255538580|ref|XP_002510355.1  Ricinus communis
might join with CYP721A JcCD0092113.10
no ESTs

MILAILFLYLLKLVYSILWVPHKTQRHFRKQGIQGPDYRPIIGNSSEMKLKREEAKSKVSDDHS

ILNRVVPAYSTWSRMYGKNYLYW (2)

 

>CYP721A JcCD0092113.10 + 

BABX01144722.1

55% to CYP721A1   Arabidopsis thaliana

JHS13D13f.10 - 

BABX01121638

63% to CYP721   JcCB0108161.20
63% to CYP721A30 XP_002510355.1  Ricinus communis
70% to CYP721A2v1 cottonwood
overlap is identical to CYP721A JcCD0092113.10 join

LMNTGGLYERLDNDPLSKQLLGEGLPELRGEKWAVHRRISNLALNLEQVK (0)

GWVPKMAASTMKMLDKWELKRAGRDEFEIEVHKDIHNLSADIISRTI

FGSSFEEGKHIFELQEQQMHLFFASLTSVYIPGFR (2)

FLPTKKNKERWRIEKEIRGS

ILNLIEANKTSAEDSRNLLSLLMSSHKNHDGKEDRLEVEEIIDECKTFYFAGKETSANTL

TWALILLAMHQDWQTKAREEVILVCKEDEHPTADNLSQLKI (0)

INMIINETLRLYSPVAMLM

RRTCKDVKLGGVEIPRDSQLALVTIATHHDLDIWGEDADKFNPDRFSEPRKHLASF

FPWGVGPRICVGQSFDMVEV

 

 

CYP734 family (probably only one gene)

 
>CYP734A26 BABX01068676 N-term

JcCB0238321.10 +

83% to CYP734A13 Vitis
89% to CYP734A26 XP_002524439.1  Ricinus communis (ortholog)

MEVELFLYWLKLVGICFFILVIVLKIVVLLWWRPRRIEEHFSKQGIRGPPYRFFIGNVKELV

EMMLKASSQPMPFSHNILPRVLSFYHHWKKIY (1)

GATFLVWFGPTGRLTVSDPELIREIFTTKAEFYEKIEAHPLVKQLEGDGLLSLKGEKWS

HHRKIIIPTFHMENLK (0)

LLVPVVANSVIEMLEQWSKMSKSNEVEIEVSEWFQTLTEDVITR

(82 aa gap)

CYP734 JcCB0513991.10 –

84% to 734A13 Vitis 

GLMIQASKSCPNVTVNDIVEECKSFFFAGKQTTSNLLTWTTVLLAMHPQWQVQAREEVLR

VCGSRDIPTKDDVVKLKTLGMILNESLRLYPPTIATIRRSKTDVELGGYKIPRGTELLIP

ILAVHHDQSIWGNDANEFNPGRFCDGVAKAAKHSIAFIPF

 

CYP735 family (probably only one gene)

 

>CYP735A22

No ESTs

>BABX01044566.1

18 KVAYDTISCYFLTPRRIKKIMEKQGVRGPEPRPLTGNILDV & 58

59 PSFVSQSTSMDCDSIHHDIVDRLLPHYVAWSKQY (1) 92

BABX01103809.1

93 GKRFIYWNGIEPRMCLTETELIKELLTKYSIKAGKSWLQQQGSKHFIGLGLLMANGDNWY

HQRHIAAPAFMGDRLKVF 170

(9 aa gap)

JcCB0951161.10 +  

BABX01115192.1

180 NMLQSLENAVKSGETEFEIGEYMTRLTADIISRTEFDCSYEKGKQIFHLLTVLQRLCA

QASRHLCFP 246

(20 aa gap)

267 PSKYNREIKSLKMEVDRLLMEII 289

(35 aa gap)

BABX01059258.1

324 FSLNLQLIMDECKTFFFAGHETTALLLTWTVMLLASNPSWQEK () 366

367 VRDEVHQVCNGQTPSTDHLPKLTL 390

(30 aa gap)

BABX01055872.1

421 LLIPKGLSIWIPVLAIHHSEELWGKDANEFNPDRFASKTFAPGRHFIPFAAGPRNCIGQT 480

FAMMEAKIILAMLISRFSFTYFSTTYR 506

 

>CYP735A22 JcPR09FLNIW.10 + 

BABX01055872.1

96% TO CYP735A22 XP_002516723.1  Ricinus communis (ORTHOLOG)

LLIPKGLSIWIPVLAIHHSEELWGKDANEFNPDRFASKTFAPGRHFIPFAAGPRNCIGQT &

FAMMEAKIILAMLISRFSF

 

CYP749 family (14 sequences, only 4 are complete) probably 9 genes and 2 pseudogenes

 

>CYP749A JcCB0359191.10 - 

58% to CYP749A1v1   Populus trichocarpa
69% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis
66% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis

complete

KFFNKLWWTPIRIQSMMRSQGIQGPSYQFFHGNSKEVFNMTKNSSINPLELSDSQDLLYT

VLPHLYSWMKLYGTNFLYWQGPRAHLVITEPEMIKEILNNRDGAFPKIEPDDYVKKLLGD

GTVTARTPEKWFRLRKLSNHAFHAECLKGMIPAMIASVEMMLQRWKICGCMEIDAFQEFK

VLTSEIISRTAFGSSYLEGQNIFDKLMRLGVIISRNKYKVKVPGISNLVKTEDDIESEKL

EQGIQNSIISMIKKREQATITEKSDSFGNDFLGLLLKAHHDDRDAHKTKRLSVQDIIDEC

KTFYVAGHETTTSSLSWIVFLLAVHTDWQDKARNEILQLFDLQNPSPDGLSRMKIVSMII

NEALRLYPPVAAFPREVQRQVRLGKLNLPANIEVEIPIIAIHHNPQIWGEDVHLFKPERF

AEGLAKATNNNVNAYLPFALGPRSCVGSNFSIMETKIALSMILQRYHFTLSPTYAHSPVN

LLTVCPKYGLQILLSSTVIGGMNI

 

>CYP749A JcCA0020391.20 + missing N-term

BABX01011654

56% to CYP749A1v1   Populus trichocarpus
69% to CYP749A25 EEF50711.1  Ricinus communis (ortholog)
next best 59% to CYP749A26 EEF50709.1  Ricinus communis
missing the N-term, not present in 9700 bp upstream
no ESTs to extend seq.

KLFGDGLVTTKGEKWIKQRRLANHTFHGXKLK (0)

NMIPVMITRVEMMLGRWRQYENKEIDVWKEFKQLTSDVISRTSFGSSYSEAQDIFDMIMEL

LILASQNSFEVRIPLIKKFIKTKDDIESDKLEQRLRESILKMIRKREEEAKMAEPDSHGS

DFLGVLLKANQDPNKSNKISINDIVDEYKTFYIAGQETTSAALNWTIFLLAIHIDWQEKV

RKEVLELFGRRNPTLNEINKLKIMNMVVNETLRLYAPVTNLLREVPKGSKLGKLLAPARM

DILIPPLAVHHDPDIWGEDVHLFKPERFAEGVAKATKGNIGAFVPFSLGPRNCVGMNFAM

TETKIALSMILQRYSFTLSPNYVHSPVVLIGVIPKKGLQITLQALEDQSS 

 

>CYP749A JcCB0047971.20 +

BABX01085129.1 runs off the end at HLI
59% to CYP749A1v1   Populus trichocarpa
75% to JcCB0285341.10
70% to CYP749A25   gi|223549222|gb|EEF50711.1  Ricinus communis (ortholog)
next best 59% to CYP749A29   gi|223549217|gb|EEF50706.1  Ricinus communis
no ESTs

MIIFVSGCVFVCFVTIALIKFFYEVWWNPTRIQSAVRSQGIKGPSYRFLYGNAKDIIR

MRDESFNSSMELSHQVFPRLQPHYYLWSKLHGKNFINWLGPRPQLIVTEPQLVKEILNNK

DELYPKPEFFTYMTKLFGDGLVTTKGEKWVRQRKLANHAFHGEKLKDMIPAMITSVEMML

RRWRQNEHKEIDVWKEFKLLTSEVISRTAFGSSYLEGQHIFDMLMELVLLLSKNGYEVRI

PIIKKFIKTKDDIKSEKLEQGVRDSILKMIKKREEEASEMDSYGSDYLGVLIKANQDPDK

TKKISIDDLVDECKTFYIAGQETTLSLLTWTIFLLAIHTDWQEKARKEVLELFGPRSPTS

DEINKLKIVSTLYHLI

 

>CYP749A JcCB0153911.10 -  C-term, last exon for JcCB0047971.20

BABX01063123.1 runs off the end at NETLR
63% to CYP749A1v1   Populus trichocarpa
84% to CYP749A25   JcCA0020391.20
78% to CYP749A25 EEF50711.1  Ricinus communis (ortholog)
next best 64% to CYP749A29 EEF50706.1  Ricinus communis
no ESTs

XXXXXNETLRLYSPITTLIREVQKGSKLGKLLAPARMDLLIPTLGVHHDPDIWGEDVHLFKPERF

AEGVAKATKNNIAAFVPFGLGPRSCVGMNFAMAETKIALSMILRRYRFTLSPTYIHSPIV

VIGVSPKKGLQIILQALEDQNC*

 

 

>CYP749A JcCA0306671.10 +

BABX01023138.1

57% to CYP749A1v1   Populus trichocarpa 
65% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
64% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis

complete

MLGSAVMETVKLIYISSSTVFAILISFLIKFFNKVWWTPMRIQSMMMSQGIKGPSYKLFY

GNNKEIIHLTNKASSNPMELSHQVFPTVLPHFHSWMKLY

GKNFLRWQGPQAYLVFSEPNELVKEIFNNRDGAFPKPE

PDNYTKKLIGEGIGTTRIPEKWLKLRKLSNHAFHAECLKSMIPAMITSVEMMLEKWRHNE

GKEIEVFQEFKVLASEIISRTAFGSSYLEGQRIFDMINQLTLIIGRNRYRIRIPGIRNLV

KTWDDIESEKIDQEIRDSIMNMIKKREEAAMMSQSNGFGTDFLGLLLKAHHDNNDPDQTK

RLSVEEMIDECKTFYVVGHETTATSFTWTVFLLAIHTDWQDKARKEVLELFGQQSPSSDG

LSKMKLGSMIINEALRLYPPVLSIPKAVQRQVKLGKLVLPANIVIEIPVIALHHDPEIWG

ENVYLFKPKRFAKGLAKATNNNLNAYLPFSLGPRSCVGYNFAITETKIALSMILQHYRFT

LSPTYVHSPVNRLTVCPQHGMQIMLHPL

 

>JcCA0080541.20 +  pseudogene

BABX01030009.1
55% to CYP749A1v1 Populus trichocarpa
64% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
89% to CYP749 JcCA0306671.10
complete

MLGSAVMETVKLIYISSCTVFAILISFLIKFFNKV*WWTP

MRIQSMMMSQGIKGPSYKLFYGNNKEIIHLTNKALSNPMELSHQVYPTVLPHFHSWMKLY

GKNFLRWQGPQAYLVFSEPDELIKEIFNNRDGAFPKPETNDYTKKLIGEGIGTTRIPEK

WLKLRKLSNYAFHAECLKSMIPAMITSVKMMLEKWRHNEGKEIEVFQEFKVLASEIISRTA

FGSSYFEGQRIFDMINQLTLIIGRNRYRIRIPGIR (2)

NLVKTRDD  &

EAEKIDQEIRDSIMNMIKKREEAAMMSQSHGFGTDFLGLLLKAHHDNSDP

DQSKRLSVEEMIDECKTFYVVGHETTATSLTWTIFLLAIHTDWQDKARKEVLELFGQ*NP

SSDRLSKMKI (0)

VSMIINEALRLYPPVLSIPKAVQRQVRLGKLVLPANIIIEIPVIALHHDPEIWGEDVCLF

KPDRFAEGLAKATNNNVNAYLPFSLGPRSCVGYNFAIIETKIALSMILQHYRFTLSPTYV

HSPVNLLTVCPQHGMQIVLHPL*

 

>CYP749A28a JcCB0184751.10 -

BABX01065070.1

73% to CYP749A28 EEF50707.1  Ricinus communis (ortholog)
next best is 66% to CYP749A29 EEF50706.1  Ricinus communis

no ESTs

CYP749A28b JcCB0098201.20 -  join with JcCB0184751.10

BABX01117872.1 last exon

65% to CYP749A1v1   Populus trichocarpa
66% to CYP749A29   JcCB0158131.10
80% to CYP749A28   gi|223549218|gb|EEF50707.1  Ricinus communis ortholog
next best 66% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis
complete

MVMENSVIYLLGCFCLYFLLILGKFLHKQWLVPICIQSIMRSQGIKGPSYRFLHGNTKQI

AKMRKEAIYNPMELSHHILPRIQPHIYSWMKLYGMNFLLWYGARAQLTVTEPELVKEILN

NKEETYCKIGFQDYIKMVLGDGLVLSTGEKWLKMRKLANYAFHGENLKGMVPAMLASVEM

MVERWRHHDGKEIEVFQEFKVLTSEIISRTAFGSSYLEGEEVFDMLKKMIFIVARNHYKV

RIPCPGIKRFLKTGDELELDKIQKEIRDTIINMIKKREKATMMGELDGYGSDFLGLLIKA

YNDPDKNKRISIDDMIDECKTFYVAGQETTASSLTWTVLLLAIHTDWQDEARKEVLELFG

QQNPTADGVARLKT (0)

MNIIINESLRLYPPVFHVTREVQREVRLGQLNIPNKIETYIPTLALHTDYKIWGEDAYLF

KPERFAGGVAKATNNSVSSFLPFGLGPRTCVGLNFAITEKKIALSMILQRYKFTLSPSYV

HSPVQILTMCPQHGVQVILEAL*

 

>CYP749A29 JcCB0158131.10 + 

BABX01063409.1 runs off the end at LKM

76% to CYP749A29   gi|223549217|gb|EEF50706.1  Ricinus communis (ortholog)
next best 64% to CYP749A25 gi|223549222|gb|EEF50711.1  Ricinus communis

no ESTs

LKMRKLAHQAFHGESLKGMVPAMVASVETMLDRWRQNEVKEIEMFQEFKILTSEIISKTA

FGSSYLEGKNLFDMLTKMATIIARNNFQVRIPGIKKILKTPDDIESEELERGMRESILMM

MKTREEGVVKGKLENYGSDFLGLLLKVYHANDLSNKISVQDLIDECKTFYLAGHETTVSS

LTWTILLLAIHPIWQDKAREEVIKLFGKQNPGSDGIARLKIMSMIVNESLRLYPPVFNIT

REVQREVRLGNVIIPEKMAVCLPILAVQHNSKVWGEDVNLFKPERFADGVAKATKNNIAA

FLPFGSGARSCVGSNFATAEIKIALSMILQNYRFTLSPTYVHAPIHVLTICPQYGLQILL

EAL

 

>CYP749A JcCB0285341.10 +

BABX01071731.1

64% to CYP749A25 gi|223549222|gb|EEF50711.1  Ricinus communis
75% to JcCB0047971.20
no ESTs

MIICVSSWVFVCFFIFVLIKFLYEVWWIPIRIQAALRSQGIKGPSYSFLYGNVKDTIR

MRDESINSSMELSHQMFSRIQPHYYLWRKLHGKNYISWLGPRPRLVVTEPQLVKEILNNK

DEMYPKPEFESYMIKLFGDGLVTTKGEKWIKQRRLANHTFHGDKLKNMIPVMITSVEMML

GRWRKYENKEIDVWKEFKQLTSDVISRTAFGNSYSEAQDIFDMIMELLILASQNSFKEIY

KNKDDIESDKLEQRLRESILKMIRKREEEAKMAEPDSHGSDFLGVLLKANQDPNKSKKIS

INDIVDEYKTFYIAGQETTSAALNWTIFLLAIHIDWQEKVRKEVLELFGRRNPTLNEINK

LKIMNMVVNETLRLYAHSTDGT

 

>CYP749A JcCB0547401.10 -  pseudogene

JcCA0103271.20 +  from same pseudogene

BABX01000377.1

BABX01089312.1

64% to CYP749A26 gi|223549220|gb|EEF50709.1  Ricinus communis

NO FIRST EXON, 2 frameshifts

(1) GPQAYWVFSEPDELIKEIFNNRDGAFPKPEPDDYTKKLIGEGIGTTRIPEKWLKLRR

LSNHAFHAECLK (0)

SMIPAMITSVEMMLEKWRHNGGKEIEVFQEFKVLASEIISRTAFGSSYLEGQ

RIFDMINQLTLIIGRNRYR &

PGIR (2)

NLVKTRDDIESEKIDHVIQNSIMNMIKKREEAAMISNQ &

SNGFGTDFLGLLLKAHHDNNDSDKTNRISVEEMIDECKTFYVVGHETTATSLTWTTFLLG

IHTDWQDKARKEVLELFGQQNPSSDTLSKMKI (0)

VSMIINEALRLYPPVLNIPKAVQRQVRLGKLVLPANIIIEIPVIALHHDPEIWGEDVCLF

KPDRFAEGLAKATNNNVNAYLPFSLGSRSCVGYNFAIXETKIAXSMILQHYRFTLSPTYV

HSPVNLLTVCPQHGMQIVLHPL*

 

>CYP749A JcCB0587401.10 –

BABX01092091.1 runs off the end at PCS

59% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
59% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis
No ESTs

METLQAIYISSCIVCVILLSFLIKFFNKVWCTPMRIQSMMRSQGIKGPSYKLFYGNIKEI

IHMTKRASSNSMELSHQVFPTVLPHFQSWMNLYGKNFLWWEGPRAHLVFSEPELIKEIFN

NKDGAFYKPQPDNGYLQKLLGDGLAMTRVSEKWLKLRKLSNHAFHAESLKSMISAMVTSV

EMMLERWGHHEGNEIDVFKEFLVLGSEIICRSAFGSSYSEGQRTFDKIIQLILIIDRNKY

RIKIPGIRYLMKTRDDVESDNIQNGIRNSIMNMIKKREEAAMFSQSNDYGSDFLGLLLKA

HHDSDMTKRLAVEEIIDECKSFYHAGQGTTATLLSWIVFLLAVHTDWQAKARKEVIELFG

QKNPSPDSLSRMKIVSVRNLKPCS

 

>CYP749A JcCA0298201.10 –

BABX01022198.1 runs off the end at KIVS

54% to CYP749A1v1  Populus trichocarpa
73% to JcCB0587401.10
59% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
no ESTs 

METLQVTYISSCTACVILLSFLIKFFNKVWGTPMRIQSMMRSQGIKGPSYKLFYGNTKEI

LRMNKKALTDTKKELSSHQVFPTILPHLHSWMKLYGKNFLWWEGAQAFLEISEPNLIKDM

MENRYGVFHKPIPDDYNRKLLGDGIAITRVPEKWLKLRKISNHAFHAECLKNMFPAMITS

VEMMIERWKHHEGKEHEVFQEFILLSSEIISRTAFGSSYLEGQRIFDMLTQLIFIIDRNK

YRIRIPGIR (2)

YLVKNRDDIESDKIDIGIHNAILNMIKKREEEAMMSQSNGFGSDFLGI

LLKAHHDNNDSDETNRISVEEIIDDCKIFYAAGQGTVATSLTWTLYLLSIH

TDWQEKARKEVFELFGQKNPSPDSLSKLKI (0)

 

>CYP749A JcCB0781971.10 +  last exon

BABX01105310.1

62% to CYP749A1v1  Populus trichocarpa
83% to CYP749 JcCA0306671.10
86% to JcCA0080541.20
74% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
73% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis

MIINEALRLYSPVVTLPREVRRQVRLGNLFLPANIIIEIPVIALHHDPEIWGEDVYLFKP

ERFAEGLAKATNNNVNAYLPFSLGPRSCVGYNFANTETKIALSMILQRYRFTLSPTYVHS

PVQILAMCPQHGMQIVLHPV*

 

>CYP749A JcCB0217801.20 – C-term

BABX01067210

55% to CYP72D1   Populus trichocarpa
66% to CYP749A26   gi|223549220|gb|EEF50709.1  Ricinus communis
63% to CYP749A27   gi|223549219|gb|EEF50708.1  Ricinus communis
70% to CYP749   JcCA0306671.10

GPRHCVGDKFALTEAKITLSMILQHFRFNLSPNYVHSPIPLLTIYPEHGMKIMFHPLQV* 

 

CYP74 clan

 

CYP74 family (3 sequences)

 

>CYP74A27 GT979878, GT978442.1, GT977155, GW617132.1, GT229029.1

Contig1362Allene oxide synthase

= fifth most highly expressed transcript in germinating endosperm

90ESTs found for Jatropha[orgn] AND "allene oxide synthase"

JcCB0380331.10 - allene oxide synthase CYP74

65% to CYP74A1 Arab.

89% to DQ340980.1 Hevea brasiliensis latex allene oxide synthase (AOS)

85% to CYP74A27   gi|255538510|ref|XP_002510320.1  Ricinus communis (ortholog)

71% to X78166.2 Parthenium argentatum mRNA for rubber particle protein

3 aa diffs to JcCB0380331.10 in overlapping region

complete

MSSSSLPSFSLQSQLQSLKNSSQQLSTRRRSS

VAPISASVSEKPSVTNSPVTVSPTESTKLPLRKIPGDYGLPFIGPIKDRQDYFYNQGKEEFFKSRA

QKYQSTVFRVIMPPGPFIAFNPRVVVVLDGKSFPVLFDVTKVEKKDLFT

GTFMPSTDLTGGYRILSYLDPSETKHTQLKNLLFYLLKSRRDYVIPEFSSTYNKLFDGLE

KDLASKGKIAFNDPGEEAAFSFLGRCYFGVDPVDTKVG

TDGPSLIAKWVLFQLAPILTLGLPAFLEEPLIHTFRLPAFLIKKDYQKLYDYFYSSAGSV

LEEAEKMGISKDEACHNILFATCFNTFGGMKIFFPNILKWIGRAGVKLHTQLAEEIRSVL

KSNGGKITMAALEQMPLMKSAVYEAFRIEPPVPAQYGKAKRDLIIESHDAAFEVK

KGEMIFGFQPFATKDPKIFDRPEEYVPDRFIGEGEKLLQHVLWSNGPETEQPTVG

NKQCAGKDFVVFISRLFVVELFRRYDSFEIEVSSSALGSSITITSLKRASF*

 

>CYP74B18

JcCB0253921.10 - short

63% to CYP74B2 Arabidopsis, N-term

GT981413.1 = JGCCJG2055C12.b

GW878141.1, FM896436.1

CYP74B BABX01099317.1

JcCB0697851.10 +  

82% to CYP74B ricinus communis (ortholog)

probably C-term for JcCB0253921.10 gene

finished

JcCB0262151.10 - 

52% to CYP74B2   Arabidopsis thaliana
100% to CYP74B

MATVMTKMMNVPPGTSPSFPSSSSSFSPPQTISTQSSPSPSLPLRKIPGSYGWPLLGPIS

DRLDYSWFQGPETFFKKRIEKYKSTVFRTNVPPTFPFFTDVNPNVVAVLDCKSFSHLFDM

EVVEKKNVLVGDFMPSLKFTGNIRTSVYLDPSEPEHGK

VKRFAMDVLKRSSKVWLTELLTNLDTLWDTVDKEISEKGKASYLFPLQQC

IFKFLSKSLAGADPAKSPEIEKSG

SDTTGLQEKLRKEARENGGSSLTFDSVNDMPLIQSVVYETLRLNPPVPLQYARARKDFKL

SSHDSMFEVKKGELLCGYQKLAMQDSKIFDDPENFKPDRFMGSGKELLNYLYWSNGPQTG

SPSESNKQCAAKDYVTLSASLIVAHMFRRYDSVTGDSSSITAVQKART*

 

>CYP74C

JcCA0087981.10 + allene oxide synthase CYP74

75% to CYP74C8 Populus trichocarpa

44% to CYP74A1 Arabidopsis aa 92-377

BABX01030838.1 (WGS)

no ESTs

JcCB0164291.10 + allene oxide synthase CYP74

54% to CYP74A1 Arabidopsis aa 414-516, no ESTs

BABX01063767.1 (WGS)

Short finished

Note: current Ricinus genome assembly does not have a CYP74C

HNSTIIKTNMPPGPFIAKNPKVIALLDAISFPLLFDTSKVEKKNVLDGTFFPSVSFTGGY

RVCAYLDPSEPNHTKLKSFFLSILSSKHNIFIPLFTTCLRQLFIDIEEEMSSKNKAYFNS

LSDSMSFNFVFRLFCDKDPLNTKIGSKGPSVLDKWLALQLAPLATLNLPKFFKYFEDFLL

HTFPLPFFLVKSDYGKLYESFYEHSGSVLDKAESFGIKRDEACHNLVFLAGFNAYGGMKV

FFPTMIKWVAQAGEKLHTQLAHEIRTVRKDGSVITFQALDKMPLTKSVVC

EALRIEPPVPFQYGKAKEDIIVHSHDAAYQIKKGE

MIFGYQPLATKDPKVFHNPHEFVGHRFVGEEGEKLLKYLYWSNDREMVNPTAENKQCPGK

DLVVLLGMVMLVEFFLRYDTCTVETKKGLGLASSVTFTSLTKATST* 

 

CYP85 clan

 

CYP85 family (2 sequences plus one pseudogene)

 

>CYP85A1 JcCA0146101.10 –

BABX01005202.1 

85% to CYP85A1    Vitis vinifera
91% to CYP85A1   gi|223534738|gb|EEF36429.1  Ricinus communis
78% to CYP85A1    Solanum lycopersicum
complete

MAVFLVILGVILFLLCFSSALLKWNEVRYRKKGLPPGTMGWPVFGETTEFLKQGPNFMKN

QRARYGSLFKSHILGCPTIVSMDPELNRYILMNEAKGLVPGYPQSMLDILGKCNIAAVHG

STHKYMRGAVLGLISPSMIREHLLPKIDQFMRTHLSNWDNKIIDIQHKTKEMALLSSLKQ

IAGSDSSSINQAFMPEFFKLVLGTLSLPIDLPGTNYRRGFQARKNIVTLLRQLIEERRLS

KESHKDMLGCLMKSEENRYKLTDEEIIDQIITILYSGYETVSTTSMMAVKYLHDHPAVLQ

ELRNEHLAIRERKKPEDPIDLNDLKSMRFTRAVIFETSRLATIVNGVLRKTTEEMELNGF

VIPKGWRIYVYTREINYDPYLYSDPLSFNPWRWMDKSLESQNYLFIFGGGTRQCPGKELG

IAEISTFLHYFVTRYRWEEVGGDNLMKFPRVEAPNGLHIRVSSY

 

>BABX01104851.1 possible N-term for JcCB0061491.10

GW881810.1 Seed specific Normalized cDNA library
Finished
69% to CYP85A1 gi|223534738|gb|EEF36429.1  Ricinus communis
 

MAVLVMVFGVGLFMGLCVLFAMFLKWNEIMYARKGLPPGTMGWPVFGETIEFLKFGPDFM

KNQRARY

(106 aa gap)

CYP85A JcCB0061491.10 +

BABX01094265.1 frameshift at &

66% to JcCA0146101.10

73% to Vitis vinifera XM_002273577.1

no ESTs

MALFIGFKQILETESGSIYESLKSEFDKLLLGTLSLPINIPGTNYYSGFQGRKRTVKILK

QIIQERRASSITHNDILGHLLNSKDPKYQLNDQEILDQIITILYSGFETVSTTTMMAIKY

LYEHPGVLQELRAEHLMIRRRKKAGEMLDWDDYKSMNFTRAVIFETSRLATVVNGVLRKT

TKDIEFNGFYIPKGWRIYVYTREINYDPSLYPEPLTFNPWRWK (0)

DKSLESQNYLFIFGGGTRQC

PGKELGIVIISIFLHYFVTQYSWEEVGGNEILKFPRVEAP &

NGFHIRVSKY*

 

>BABX01064456.1 pseudogene N-term for CYP85A

65% to CYP85A1   gi|223534738|gb|EEF36429.1  Ricinus communis

finished

4564  MAVLVAVFGVGLFMGLGILFTMSLKWNEIIYARKGLPPGTMGWPLFGGTIEFL*FGPDFT  4385

4384  KNQSGMY

 

CYP87 family (7 sequences, 4 are mostly complete)

 

>CYP87A29 JcCB0025421.10 + 

BABX01069681.1

75% to CYP87A9 Medicago truncatula
82% to CYP87A7 Populus trichocarpa
cannot detect the N-term
84% to CYP87A29   gi|255537303|ref|XP_002509718.1  Ricinus communis (ortholog)
finished

IIFYKR

WVYRWRNPRCNGKLPPGSMGLPLLGETFQFFEPNFSADIPPFVKKRMKK (2)

YGPIFKTNLVGRPVVVSTDPDLNYFIFQQEGQLFQSWYPETFTQIFGRQNVGSLHGFMYKYLK

KMVLNLFGPEGLKKMLPEVQKTTYDRLWLWSHQDSIELKEATASMIFDLTAKKLISYDE

NNSSENLRENFVAFMQGLISFPLEVPGTAYHKCLQGRKRAMKMLKNLLHERRAKPRKHQS

DFFDFVLEELQKEGSILTEAIALDLMFVLLFASFETTSLAITMVIKFLSDSPLALKQLTE

EHEAILRNRENANSSITWKEYKSMTFTFQVINETVRLANIVPVIFRKVLTDIQFKGYAIP

AGWAIMVCPPAVHLDRAKYEDPLTFNPWRWEGREITGASKHFMAFGGGMRFCVGTEFTKV

QMAVFLHCLVTKYSWQVIGGGNTVRTPGLQFPNGYHVQFMEKETGFKKY

 

>CYP87B15 JcCB0082131.10 - 

49% to CYP87D1   Populus trichocarpa
84% to CYP87B15   gi|255570414|ref|XP_002526166.1  Ricinus communis (ortholog)

complete

MWPLGLFIVAFFVMYIGNWVYKWRNPPCNGTLPPGSMGFPLIGETIQFFIPSKSLDMPPF

VKNRIKKYGPMFKTSLAGRPVVVSSDADFNYFILQQEGKLVELWYLDSFAELLGQNGSLK

EGFIGYIHKHLKKVISEHFGPERLKGKLLPQLEEMVNHSLQAWTMQESVEVKHECSKMIL

DFTSKLLFSYDSEKQGKSLSETFASFIQGLFSFPLNIPGTAFHKCMQNQKKILKTIRKTI

EERRACPGTRREDFLGRLIEEMKAENLLNDDLVSFVIFALLLATSETVPSTLTLAIKLLT

EHPLVMQELVKENEEIICNRENKETGLTWKEYKSMTFTMHVINEALRMSGSVGILRRTIE

DIDINGYTIPKGWTIMIVPSSLHLNPDIYKDPLAFNPWRWKDLGPNVRAKNFIPFGGGMR

SCGGAEFSKVLVAVFLHAFVTKYRLTNVKEGEIVRTPMLGFGNGHYIKVSKKPEMETEN

 

>CYP87D JcCB0397291.10 –

64% to CYP87D1   Populus trichocarpa
57% to CYP87D14   gi|255573862|ref|XP_002527850.1  Ricinus communis
65% to CYP87D10 cottonwood

complete

JcCD0104599.10 - 

BABX01125511 SAME AS CYP87D JcCB0397291.10 C-TERM

BABX01079390 

MWIIGLCFASLLVIYCTHFFYKWRNPKCKGVLPPGSMGLPIIGETLQLIIPSYSLDHHPF

IQKRIQRYGPIFRTNLVGRPVIVSADPEVNQYIFQQEGNSVEMWYLDAYAKIFQLDGESR

LSAVGRVHKYIRSITLNNFGIENLKENLLPQIQDLVNQSLQKWSNKASVDVKQAASVMVF

NLTAKQMFSYGVEKNSSEEMTEKFTGIFNSLMSLPLNIPGTTYHKCLKDREAMLKMLRDT

LKQRLSSPDTHRGDFLDQAIDDMDTEKFLTGDCIPQLIFGILLAGFETTATTLTLAFKFL

AEHPLVLEELTAEHEKILSKRENLESPLTWDEYKSMTFTHHVINETLRLANFLPGLLRKA

LKDIQVKNYTIPAGWTIMVVKSAMQLNPEIYKDPLAFNPWRWKDLDSYTVSKNFMPFGGG

SRQCAGADYSKLFMTIFLHVLVTKYRSV

 

>CYP87D JcCA0251181.10 - 

BABX01016949.1

56% TO CYP87D   JcCB0397291.10
52% to CYP87D1 Populus trichocarpa
63% to CYP87D14   gi|255573862|ref|XP_002527850.1  Ricinus communis
ESTc GW613938.1, GW613366.1
complete

MLMTELLFGAFFIIFLTYWINRWRNPKCNGVLPPGSMGLPLLGETLQLLIPRYSLDLHPF

IRKRIQRYGPIFRSNVAGRPIVFTADPELNHYIFIQERRLVELWY

MDTFSNLFVLDGESRPTGATGYIHKYMRGLFLTHFGAERLKDKLLHQIQELIHTTLQSWC

KQPTIEVKHAASAVICDFSAKFLFGYEAEKSPFNMSERFAKFAESLVSFPLNIPGTAYHQ

SLEDREKVMKLLKNVLRERRNSTKKSEEDVLKQILDDMEKENFITDDFIIQILFGALFAI

SESIPMTIALLVKFLSAQPSVVEELTAEHEEILKNKKEKGLDSSITWEDYKSMTFTLQVI

NETLRIANVAPGLLRRTLRDIHYKGYTIPAGWTIMVLTSSRHMNPEIYKDPVEFNPWRWK

DLDSQTISKNFTPFGGGTRQCAGAEYSRAFISMFLHVLVTKYRYISPPCLCLIFH

 

>CYP87D JcCA0041331.10 + pseudogene

61% to CYP87D11 Populus trichocarpa
BABX01025504.1
85% to JcCA0251181.10
55% TO CYP87D   JcCB0397291.10
58% TO CYP87D14 XP_002527850.1  Ricinus communis
no downstream exons in 4000 bp

MLMTELLFVAFFTICLTYWINKWRNPKCNGILPLGSMGLPLLGETLQLLLPRYSLDLH

PFIRKRIQR (2)

YGPIFRSNVAGRPIVFTADPELNHYIFSQDR &

SIELWYMDTFSNLFVLDGESRTGGATGFIHKYLRGLLLTHFGAERLKEKLLHQMPDLVHI

SLQSWCKQPTIEVRQAASA (0)

VVCDFTAKFLFGYE

 

>CYP87D JcCB0748311.10 +

BABX01102741.1

49% to CYP87D1   Populus trichocarpa
58% to JcCA0251181.10
61% to CYP87D14 XP_002527850.1  Ricinus communis
NO ESTs  

SFPLNISGMTYYQSLK (0)

SRERAFEIFKKAVKDRINSVEKSRDDVLDQALTDMEREKFLTEE

FIQFIFFGVLFATYESISLTVALIFKFLESHPSVLAELM (0)

IEHENILKSRQDSESSITWDDYKSMTFTQQ (0)

VISETLRLGTTAPGLCRKAVKDIQFK (1)

GFTIPTGWNIMMVTSIRHINPEVYKDPLEFNPWRWK

 

>CYP87D JcCB0419061.10 -  pseudogene mid region

BABX01080635.1

42% to CYP87D1   Populus trichocarpa
46% to CYP87D14   gi|255573862|ref|XP_002527850.1  Ricinus communis
69% to JcCA0251181.10

QSWCNQPAMKLxxxxxx

VVCDFSAKFLFGYEAEKSP*KLSEKFAKFSE &

QSFFSFPLNIPGTVYHKSLK (0)

NRKKVMKILDDVLREKRSSTKKSEEDVMSQTLSDMEKEDFITEDFIIQIMFG

ALFAISESIPTSIKIIFKLLSDQPSVIEELT

 

CYP88 family (3 sequences)

 

>CYP88A41 JcCA0285021.10 - 

71% to CYP88A11 Glycine max

78% to CYP88A41 XP_002526524.1 Ent-kaurenoic acid oxidase Ricinus (ortholog)

GW617093.1 C-term

Complete

JcCD0180051.10 -

BABX01136174.1

100% to CYP88A JcCA0285021.10 C-term EXOT

MHLEVDSTWAILLAVVLGVLGSLKWILKKVNWWLYETKLGEIQYSLPPGDLGWPFIGNMW

SFLKAFKSTDPDSFMRNFVARYGGGGVYKALMFGNPSVLVTTPETCRKVLTDDEAFQPGW

PKSTADLIGKKSFVSISYEEHKRLRRLTSAPVNGHEALSQYILYIEENVISALDEWASMG

EIEFLTQLRKLTFKIIVHIFLGSESVSVMEALEKEYTSLNYGVRSMAINLPGFAYHKALK

ARKNLVAVFQSVVNERRNQRMDGRRLVSSKKKDMMDALLDVEDEKGQKLSDEEIIDILLM

YLNAGHESSGHITMWATIFLQAHPEYLKKAKKEQEEILKRRPPTQKGLTLKETREMIYLS

QVIDETLRLVTFSLTVFREAKADINLDGYIIPKGWKVLTWFRSVHLDPEIYPNPTEFYPSRWD (0)

NHTAKAGTFLPFGAGSRLCPGNDLAKLEISIFLHHFLLNYQ (2)

LERLTPESKVMYLPHSRPKDNCLARVKKITSVSM*

 

>CYP88A JcCB0742491.10 +

BABX01102253.1

70% to CYP88A11 Glycine max

91% to JcCA0285021.10

75% to CYP88A41 XP_002526524.1 Ent-kaurenoic acid oxidase Ricinus            communis 

finished

MDLEADSTWVVLLAVVLGALGSLKWILKKVNWWLYETKLGEIQYSLPPGDLGWPFIGNMW

SFLKAFKSTDPDSFMRNFVARYGGGGVYKALMFGNPSILVTTPETCRKVLTDDEAFQPGW

PKSTEELIGKKSFVSISHEEHKRLRRITSTPLNGHEALSQYISYIEENVIYALDEWASMT

EIEFLTQLRKLTFKIIMHIFLGSESISVMDDLEKEYTSLNYGVRSMVINFPGFAYHKALK

(61 aa gap)

JK317501.1 may be the end of JcCB0742491.10

BABX01014544.1

LNAGHESSGHIHNVGXILLQEHP &

PRIXQKAK ()

EEQEEIIKRRPPTQKGLTPNEVREMKYLSK (0)

VIDETLRLITFSLTVFREAKANINLN (1)

GYIIPKGWKVLVWFRSVHLDPEIYPNPKEFNPSRWD (0)

NRIVKAGTFLPFGAGSRMCPGNDLAKLEISIFLHHFLLNYQ (2)

LQRLNPECSLMYLPHSRPKDNCLARIKKIHSSSA*

 

>CYP88A JcCB0538921.10 -  pseudogene N-term

BABX01088678.1

72% to CYP88A41 XP_002526524.1 Ent-kaurenoic acid oxidase Ricinus communis
72% to CYP88A   JcCB0742491.10

MELAFTWVVLIAIILGAFGGLNWILKRANKWFYETQLREMQYSLPPGDLGWPFIGNMWSF

LRAFKSADPDSFMRTFAARF

 

>CYP88A JcCD0209899.10 - pseudogene

BABX01147144.1

77% to CYP88A JcCA0285021.10
82% to CYP88A41 XP_002526524.1 Ent-kaurenoic acid oxidase Ricinus communis

GVRAMAINLPGFAYHKAL &

K (0)

ARKNLVAVLQSTVKERKNQRKGAMSSKKRDMMDALIDVED

ENGRKLSEEEIVDVLLMYLNAGHESS

 

>JcCB0778131.10 +   

70% to CYP88A41 XP_002526524.1 Ent-kaurenoic acid oxidase Ricinus communis
86% to CYP88A   JcCA0285021.10

HESSTYTTMWATIFLQAHPEYLKKAKKEQEEILKRRPPTQKGLMLKETREMKFLSPVID

ETLRLVTFSFALFREAKVDINLDE

 

CYP90 family (4 sequences plus 2 pseudogenes)

 

>CYP90A35  (one sequence)

JcCB0094371.10 – exon 1

BABX01128559.1 exon 2

69% to CYP90A16 Vitis vinifera

GW614891.1

CYP90A JcCB0171311.10 - 

76% to CYP90A16 Vitis vinifera

85% to CYP90A35 XP_002540303.1  Ricinus communis (ortholog)

BABX01083659.1 heme signature exon to end = JcCB0461461.10 -

finished

MDFLFYFPMLVSLSAFIFVIHRTSRNRRLRLPPGNMGLPFLGETLQLISAYKTENPEPFI

DERVKKFGSLFSTHVFGEPTVFSVDPETNRFILQNEGKLFESSYPSSISNLLGKHSLLLM

RGALHKRMHSLTMSFGNSSIIRDHLLADIDRLVRLNLDSWTDRVFLMEEAKK (0)

ITFELAIKQLMSLDPGERTESLRKEYVLVIEGFFTVPLPIFSTTYRRAIQ

xARTKVAEALSLIVRERRRESEAGEKKNDMLEALLSAGDGGF

SDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEEHEGIRAKKSEGEEALE

WNDYKSMPFTQCVVNETLRIANIIGGVFRRVMTDVQIK 362

 

(0) SNSGATSPGNVFTPFGGGPRLCPGYELARVELSVFLHHLVTRFS (2)

WTPAEKDKLVFFPTTRTQKRYPINVQRRNHLKS*

 

>CYP90B21 (one sequence)

JcCB0297661.10 +

BABX01072671.1

BABX01050127.1

BABX01072671.1

86% to CYP90B12 Vitis

83% to CYP90B21 EEF40874.1  Ricinus communis (ortholog)

BABX01084131.1 frameshifted exon GYDI..

JcCA0274071.10 -  C-term

BABX01019497.1

Missing N-term

finished

RFNLPPGNMGWPFLGETFGYLKPYSATSIGEFMEQHISR (2)

YGNIYKSNLFGE (2)

PT &

IVSADAGLNRFILQNEGRLFECSYPK ()

SIGGILGKWSMLVLVGDMHRDMRIISLNFLSHARLRTHLLKEVEKQ

TLLVLNSWRENCAFSAQDEAKKFTFNLMAKHIMSMDPGKPETEQLKKEYVTFMKGVVSAP

INLPGTAYRKALQSRSTILKFIEQKMEERIGKMKGVENLEEDDLLGWVLKNSNLSTEQIL

DLILSLLFAGHETSSVAIALAIYFLQGCPSSLQQLREEHMKIARAKKQSGERLLTWDDYK

KMEFTQC

VISETLRLGNVVRFLHRKALKDVRYK

GYDIPRGWKVLPVIAAVH &

LDSSHFDQPQRFNPWRW

QNSSSRRGSSTCPSMSTTSSNYFMPFGGGPRLCAGSELAKLEMAVF

IHHLILNYHWELADNDQAFAFPFVDFPKGLPIRVKRATLCSYN*

 

>CYP90C (one sequence)

 

>CYP90C14 JcCD0065514.10 – N-term

50% to CYP90C5 Vitis, 39% to CYP90D8 Vitis

63% to CYP90C14 EEF40775.1  Ricinus communis (otholog)

GW880012.1 (includes stop codon)

GW881060.1 (includes same stop codon)

JcCD0120124.10 -  

60% to CYP90C5 Vitis

BABX01127592.1

Missing the C-term

finished

MKNMEYYYWVLIGFSIFVGYCWFLFKKENEKKKKIFKKSEVPKGNLGWPFLGESLDFISC

GYTSRPVSFMEKRKSLYGKVFKTHILGTPIIVSTDPEVNKVVLQNHGNVFVPAYPKSVRE 
LFGDFSILQMTGTQHKRLHALSAGFL*SPLLKSKITKDIEVSVKQALASWQNKHHVLLQE 
EIKKVTFQVLVKVVMGIGPGDELDFLKREFE
XXXXXXXXXXXXXXXXXXXX

(2) TKERLLKMVKKIIEERIIAMEKYEGKGIIVNDVIDLLLRDNGEENEKQ

SPLLDYICSNIIEMMIPGEETMPTAMTLAVKFLSDCPVALNQIT (0) 320

 

 

>CYP90D (one sequence plus one pseudogene)

 

>CYP90D21 JcCA0302541.20 + N-term

JcCB0457811.10 +

BABX01083366.1

BABX01011113.1 last exon

77% to CYP90D8  Vitis vinifera
BABX01022680.1 N-term
Lower case is from the pseudogene BABX01000683.1
89% to CYP90D21 Ricinus communis XM_002527368.1 (ortholog)
finished

MDNLLLIVLAILLSSIIILYRNKWFSIIIIRSSSSSSSKYRTPLPLGSLGWPFLGETLEF

VSSAYSDRPESFMDKRRRM (2)

YGKVFKSHIFGTPTIVSTDAEVSKFILQSDAKTFVPSYPKSLTELMGKSSILLINGSLQK

RIHGLIGAFFKSALLKAQITRDMQVYVQESMNKWKYDQPIYIQDETKN (0)

iafqvlvkalisldpgqemeslkkqfqefisglmslpinvpgsqlyrslqa (?)

kkkmvklvkk

iikskrehhngmslvpkdvvdvllndsneqltddliadnmidmmipgedsvpvlmtlavk

ylsdcpaalqqlt (0)

denm &

klrslkaqfgeplnwtdylslpftqk (0)

VITETLRIGNIIIGVMRKAMKDIEIKGYLIPKGWCAFAYFRSVHLDENHYDWPYQFNP

WRWQ (0)

DKDMSSCNFTPFGGGQRLCPGLDLARLEASIFLHHFVTQFS (2)

WVAEEDTVVNFPTVRMKRRMPIWVKRRE*

 

 

>CYP90D BABX01000683.1 nearly identical seq but appears to be a pseudogene

JcCA0105981.10 +  /pseudo

79% to CYP90D8 Vitis

91% to Ricinus communis XM_002527368.1

missing N-term 178 aa and last exon, no ESTs

finished

IAFQVLVKALISLDPGQEMESLKKQFQEFISGLMSLPINVPGSQLYRSLQA (?)

KKKMVKLVKK

IIKSKREHHNGMSLVPKDVVDVLLNDSNEQLTDDLIADNMIDMMIPGEDSVPVLMTLAVK

YLSDCPAALQQLT (0)

DENM &

KLRSLKAQFGEPLNWTDYLSLPFTQK (0)

VITETLRIGNIIIGVMRKAMKDIEIKEYLIPKGWCAFAYLG*IHLDENHYDWPYQFNPWRWQ (0)

DKDMSSCNFTPFGGGQRLCPGLD &

LAKLEASIFLHHFVTQFR

 

>CYP90D Ricinus communis XM_002527368.1, XP_002527414.1

MDSLLLIVLTILLSSVIILYRNCRLFSILFRSSKTKTPLPLGNL              

GWPFLGETLEFVSCAYSDRPESFMDKRRRMYGKVFKSHIFGTPTIVSTDAEVSKFILQ

GDAKMFVPSYPKSLTELMGKSSILLINGSLQKRIHGLIGSFFKSPHLKAQITRDMQVY

VQESMKNWRCDQPIYIQDETKNIAFQVLVKALISLDPGQEMESLKKQFQEFICGLMSL

PVNIPGTQLYRSLQAKKKMVKLVQRIIQSKRDGGISTVPKDVVEVLLNDANAQLTDDL

IADNMIDMMIPGEDSVPVLMTLAVKYLSDCPAALQQLTDENMKLKSLKAQLGEPLTWT

DYLSLPFTQRVITETLRMGNIIIGVMRKAMKDIEIKGYLIPKGWCAFAYFRSVHLDEN

HYDWPYQFNPWRWQDKDMSNSNFTPFGGGQRLCPGLDLARLEASIFLHNFVTQFSWVA

EEDTIVNFPTVRMKRRMPIWVKRREEDQSLSL

 

CYP707 family (5 sequences)

 

>CYP707A78 JcCA0143101.10 –

BABX01004868.1

65% to CYP707A25   Nicotiana tabacum
82% to CYP707A78 XP_002518764.1  Ricinus communis (ortholog)
next best 69% to CYP707A82 XP_002510214.1  Ricinus communis
complete

MEITSIIIYIFLSLSTLFSYLLIKKKRNESKPRAKLPPGSMGWPYVGETLRLYSQDPNVF

FATKQLRYGEIFKTHILGCPCVMLASPEAARFVLVTHSHLFKPTYPKSKERLIGQSALFF

HQGDYHNRLRKIVQGSLSPETIRKLIPDIEFFTNSTLESWANGQIVNTYHEMKKLSFQVG

ILSIFGLLSNNYREKLNENYQIMDKGYNSLLTKIPGTTYQKALLARKRLNQILGEIISER

KEKKLLEKDLLSRFLNFKDENGEILSLDHIADNTIGVLFAAQDTTASALTWILKFLHDDQ

KLLEAVKAEHMAIYEVNKRGKKSL &

TWAQTRNMPLTYR (0)

CYP707 JcCA0283241.10 +  possible end for JcCA0143101.10

BABX01020520.1 C-term exon

VILESLRMASIISFSFREAIMDVEYK (1)

GYLIPNGWKVMPLFRNIHHNPKFFPDPHIFDPSRFE (0)

VPPKPNTFIPFGNGVHACPGNELAKLEMLIMIHHLVTKFR (2)

CEVVGSGDGIQYDPFPVPQKGLPARFWPETNCLNQD*

 

>CYP707A79 JcCB0029391.10 + 

58% to CYP707A25   Nicotiana tabacum
87% to CYP707A79 XP_002518804.1  Ricinus communis (ortholog)
next best 58% to CYP707A82 XP_002510214.1  Ricinus communis
complete

MDFNSIFFLLFLSTFLIFLFHSLLKFFSPTRTQQKLPLPPGTLGWPYIGETFQLYSQNPN

VFFASKQKRYGSVFKTHILGCPCVMISSPEAAKFVLVTRAHLFKPTFPASKERMLGKQAI

FFHQGEYHTKLRKLVLRAFVPESIKYIVSDIETIAKDALKSWEGRIVNTFQEMKTYTFDV

ALLSIFGKDEFQYREDLKRCYYILEEGYNSMPINLPGTLFHKAMKARKELAQILAKTLST

RRQMKLDRTDLLGSFMGDKEGLTDEQIADNIIGVIFAARDTTASVLTWILKYLEENPSVL

EAVTKEQEEIVKSKEEGDENIFLTWADTKKMPITSRVIQETLRVASILSFTFREAVEDVE

YEGYLIPKGWKVLPLFRNIHHSPEIFPDPEKFDPSRFEVAPKPNTFVPFGNGTHSCPGNE

LAKLEILVLLHHLTTMYRWSMVGSDNGIQYGPFALPQNGLPIKLSQKLH

 

>CYP707A80 JcCA0152931.10 +

BABX01005976.1

BABX01072373.1

58% to CYP707A25   Nicotiana tabacum
62% to CYP707A79   gi|255555535|ref|XP_002518804.1  Ricinus communis
57% to CYP707A81   gi|255565166|ref|XP_002523575.1  Ricinus communis
91% to CYP707A80 Ricinus (partial seq)

CYP707 BABX01031285.1 C-term exon

possible end of JcCA0152931.10

78% to CYP707A2 Arab.

missing C-term exon
complete

MTILTLPLVFGSFLLPFLVLLLPLFLQWHHSKDKRLPPGS

MGWPYIGETLKLYTQNPNSFFSNRQKRYGDIFKTHILGCPCVMISSPEAARIVLVTKAHL

FKPTYPTSKEKMIGPEALFFHQGAYHSRLKKLVQASFLPSAIRGSVSEIEQIVLAFLPTW

KNATINTLQEMKRYAFDVAMISAFGEKQALEIQGIKHLYLCLEKGYNSMPLDLPGTLFNK

AMKARKQLNEILRRLIEKRRESGKEGGGLLGVLLGAQDEKLNNKLNDSQIADNIIGVIFA

AHDTTASVLTWVLKYLHDNQDLLQAVATEQERIRQEIIEANRRLTWDDTRRMPLTIRVIQ

ETLRRASILSFTFREAVEDVEFEGYLIPRGWKVLPLFRTIHHSADFFPQPDKFDPSRFEV

PPRPYTYMPFGNGVHSCPGSELAKLEMLILLHHLTITYR (2)

WQVMGDDDGIQYGPFPVPKRGLPIRVIPSPRSKLAEI*

 

>CYP707A81

JcCB0602461.10 + 

BABX01093346.1 possible N-term for JcCB0546421.10

85% to CYP707A81 XP_002523575.1  Ricinus communis (ortholog)
next best 68% to CYP707A82 XP_002510214.1  Ricinus communis

complete

MGMVLVCSFCCALFLLTILRLKRRQQDKIKRGNLPPGSMGLPYIGETLQLYSQNPDIFF

ANKQQR (2)

YGEIFKTRILGCPCVMVASPEAIRFVLVTRASLFKPTYPPSKEKLIGPSAIFFNQGIYH

SQLRKLVQVSLSLDVIRNLVPHIEAIAISTLESLSSTAKVVNTFHE

CYP707 JcCB0546421.10 +  

65% to CYP707A25 Nicotiana tabacum

VQFTFDAAVLSIFGNLDSYYRENLKINYYILDKGYNSFPTTLPGTSYSKSVM

ARKRLSRILGWIIRERKEKGLIQNDLLGYLLNFKDEKGQMLSDNQITDNIIGVLFAAQDT

TASVLTWILKYVHDDSKILEAIKMEQNAIYESNGRGKNPLTWSQTRNMPFTNKVIMESLR

MASVISFTFREAVEDVEYKGYLIPKGWKVLPLFRNIHHNPDFFTDPHVFNPSRFE

JcCA0152161.10 +   possible end for JcCB0546421.10

CYP707 BABX01005889.1 last exon

VGPKPNTFMPFGNGVHACPGNEVAKLEMLILIHHLVTKFR (2)

WEVVRYKSGVQYDPFPVPEEGLQAKFWKESINTAGHHFI*

 

>CYP707A82 JcCA0153431.20 - 

BABX01006033.1 C-term exon

72% to CYP707A25   Nicotiana tabacum
85% to CYP707A82 XP_002510214.1  Ricinus communis (ortholog)
next best 72% to CYP707A78 XP_002518764.1  Ricinus communis
complete

MVCGILVYFIIFLLSLFSYITKRRCKQERHKGIKLPPGSMGWPYIGETLQLYSQDPNVFF

ADKQKRYGEIFKTHILGCPCVMLASSEAARFVLVTEANLFKPTYPKSKEHLIGPSALFFH

QGDYHMRLRKLVQSSLSLDALRNLVADISAIANYTLNSWDAGHVIDTFQELKKFSFEVGI

LVIFGQLESHYREELKKNYRIVDKGYNSFPTVLPGTAYKKALTARKRLNKILSDIISERK

EKKLLEKDLLGCLLNSKDDKGQVLTDDQIADNIIGVLFAAQDTTASSLTWVVKYLHDNPK

LLEVVKAEQKAIYEMNGEGSQPLSWSQTRSMPFTQKVVLESLRMASIISFTFREAVADVE

YKGCLIPKGWKVMPLFRNIHHNPEYFSDPHKFDPSRFEVAPKPNTFMPFGSGVHACPGNE

LAKLEMLIITHHLVTKFRWELVGSENGIQYGPFPVPIHGLPARFWKESTTKL*

 
>CYP707A82 Ricinus communis XM_002510168, XP_002510214.1

MGGILVYILFFLFSLLWYITKRRNKKELSKGVKLPPGSMGWPYI              

GETLQLYSQDPNVFFADKQKRYGEIFKTHILGCPCVMLASPEAARFVLVTQAHLFRPT

YPKSKEHLIGPSALFFHQGDYHLRLRKLVQGSLSLFALRNLVADISSIAASTLDSWDG

GHVINTFQELKKFSFEVGILAIFGNLESHYREDLKKNYRIVDKGYNSFPTSLPGTPYR

KALMARKRLSKILSDIISQRKEKKLLDNDLLGCLLNSKDEQGNVLTDDQIADNIIGVL

FAAQDTTASAMTWIVKHLHDNXXXXXXXX

AEQKTIYELNDEGSQPLSWGQTRNMPLSHKVVLESLRIASIISF

TFREAVADVEYKGYLIPKGWKVMPLFRNIHHNPEYFSDSQKFDPSRFEVAPKPNTFMP

FGNGVHACPGNELAKLEMLIITHHLVTKFRWEVVGSQNGIQYGPFPVPVHGLPARFWR

ESTSC

 

CYP716 family (10 sequences, only 2 are complete)

 

>CYP716A54v1 JcCA0316961.10 - 

BABX01024285.1 8 a diffs to JcCA0225861.10

71% to CYP716A15 Vitis vinifera

98% to JcCA0225861.10

85% to CYP716A54 XP_002528002.1  Ricinus communis (ortholog)

complete

MEFIVIPFAILLTIALSIIFYKHKMGIKNLPPGSTGWPLIGESIEFLTTGRKGQPEKFIF

DRMVKFSNKVFKTSLFCEPAAVFCGAAGNKFLFSNENKLVTAWWPSSVNKIFPSSQQTSS

QEESMRMRKLLPQFLKPEALQRYIGIMDVVAQSHFAFDWDNKQQVTVFPLAKMYTFSLAC

RLFLSMEDREQVEKFAKPFDVLASAIIAIPIDFPGTSFNRGIKASNLVRKELLKIIKQRK

NDLGENKASQTQDILSHMLTTADDKGQFMSEIEIADKILGLLIGGHDTASAAITFVVKYL

AEMPQVYEKVFEEQMRIAKAKVAGELLNWEDIQKMKYSWNVACEVMRLAPPLQGAFREAI

TDFTYADYTIPKGWKLYWSANSTHRNPECFHDPEKFDPSRFDGNGPAPYTFVPFGGGPRM

CPGKEYARLEILIFMHNIVKKFKWEKLLPQENIIVDPLPIPAKGLPIVLHPHGH

 

>CYP716A54v2 JcCA0225861.10 –

BABX01014119.1 100%

70% to Ricinus communis XM_002522891.1

85% to CYP716A54 XP_002528002.1  Ricinus communis 
98% to JcCA0316961.10 allele?

N-term may be too long

missing C-term

MPLLNSFVLLFLLGKGLYKLPIYLIKHIREEK

MEFIVIAFAILLTIALSIIFYKHKMGIK

NLPPGSTGWPLIGESIEFLTTGRKGQPEKFIFDRMVKFSNKVFKTSLFCEPSAVFCGAAG

NKFLFSNENKLVTAWWPSSVNKIFPSSKQTSSQEESMRMRKLLPQFLKPEALQRYIGIMD

VVAQSHFAFNWDNKQQVTVFPLAKMYTFSLACRLFLSMEDREQVEKFAKPFDVLASAIIA

IPIDFPGTSFNRGIKASNLVRKELLKIIKQRKNDLGENKASQTQDILSHMLTTADDKGQF

MSEIEIADKILGLLIGGHDTASAAITFVVKYLAEMPQVYEKVFEEQMRIAKTKGAGELLN

WEDIQKMKYSWNVACEVMRLAPPLQGAFREAITDFTYADYTIPKGWKLYWSANSTHRNPE

CFPDPEKFDPSRFDGNGPAPYTFVPFGGGPRMC

 

>CYP716A JcPR01CTLCA.10 + pseudogene N-term

BABX01034559

68% to CYP716A54 XP_002528002.1  Ricinus communis
77% to CYP716A54v1 JcCA0316961.10

MEVIVIPFAILLIIALSLIVCKHQISNTFDIKNLPPGNTGWPLIGESIGFLTTGRKGQLE

KFIFDRMSKYSNKVFKTSLFCEPAAVFCGVAG &

NNFLFSNENKLVTVWWPSFVNKNLP &

TSSQEESIRNEKSFFCKFLK

 

>CYP716A JcCA0159901.10 – pseudogene mid to PKG motif

81% to CYP716A54 XP_002528002.1  Ricinus communis
90% to JcCA0316961.10

EESIRMRKLLL*FLKAEALQRYIGIMEVVAQRHFAFDWDNKRQVTVFPLAKMYTFSLACR

LFLSMEDREQVEKLAKSFDFLASAFIAIPIDLPGTSFNRGIKASNLVRKELLKIIKQRKT

DPAENKASQTPDILSHMLTTVDDKGQFMSEMEIADKILGLLIGGHDTASSAITFVVKYLA

EMPQVYEKALEEQMGIAKSKVAGELLNWEDIQKMKYSWKVACEVMRLAPPLQGAFREAIT

DFTYADCTIPKGW (0)

 

>CYP716A JcCB0704881.10 +  </