Lodderomyces elongisporus P450s

 

D. Nelson

 

August 9, 2009

 

10 sequences

 

>CYP51F1 Lodderomyces elongisporus

77% to CYP51F1   Candida parapsilosis

LELG_03738

MALVDLAIQGYTFFMSLTTFQQVFILIVVPFVYNIAWQLLYSLRKDRVPLVFYWIPWFGSAVGYGQQPYEFFEQCRQKYG

DVFAFVLLGKVMTVYLGPKGHEFILNAKLSEVSAEEAYKHLTTPVFGKGVVYDCPNQRLMEQKKFAKFALTTDSFRKYVP

LIREEVLNYFTSNSNFNMKNQKSGVADVMKTQPEITIFTASRSLMGKEMRSRFDESFAELYSDLDKGFTPVNFVFPNLPL

PHYWKRDAAQQKISSTYMKEITKRRQAGAIDGDEDLISSLMVHSTYKDGVKMTDQEIANLLIGILMGGQHTSASTSAWFL

LHLGDKPELQEQLYEEIQQALQSKGGNLSDLSYEDLQKMTLVNNTIKETLRMHMPLHSIFRKVMSPMVVPGTTYVVPKGH

HVLVSPGYAHTNERYYKNANQFDPHRWDNANMSNKDSDEVDYGFGKVSKGVASSYLPFGGGRHRCIGEQFAYVQLGTLLT

TFIYNIKWKLKGGHVPHVDYASMVTLPELPAEIVWEKRDTCVV

 

>CYP52A48 Lodderomyces elongisporus

73% to CYP52A38   Candida parapsilosis

LELG_04957

MSSTFVQQVASLGQEIVPYFTKWYTVLILLVVTYIAAFNIKDAYMTWKLGCKNPLYYKPAGYTGVYSLYEIIQRKNKGEL

AEFAQEAFDEYTTNTVYVKIGGLMKIMFTVDPENIKAVLATQFNDFSLGIRHAHFAPLLGDGIFTLDGDGWKDSRAMLRP

QFAREQIAHVKALEPHVLMLAKQIANTKYETFDLQELFFRFTVDTSTEFLFGESVHTLYDDKIGVAPPNDIPGREAFADA

FNFSQHYLATRTYSQVFHWLFNSKEFRENNAKVHHLAQYFVKKALNFTPEELEEKSQDGYTFLYELVKQTRNPKTLQDQL

LNIMVAGRDTTAGLLSFCMFELARNPEIWQRLRTEVLDNFGDGSTKEQIDSITFENLKKCEFLKSVLNETLRLYPSVPIN

FRTATRDTTLPHGGGSDGSKPIFVPKGSTVAYAIYKTHRLEEYYGKDSQEFKPDRWTDMKRLGWAYLPFNGGPRICLGQQ

FALTEASYVLARLAQLFPTLQSRDETYPPKKCIHLTMNHAEGVFVGMK

 

>CYP52A49 Lodderomyces elongisporus

72% to CYP52A37   Candida parapsilosis

LELG_03506

MAISLETLPLAIVQHLFTWYGAITLLITTYFTGLYIRREIFRRQRGCMEPFVYPEGGPLGIKLGYDLQDKKKKGEIVDFM

CKLYHKFDRVKTFSAYLFGVRVVVGVEPENLKAVLATQFNDFGLGTRHAHFKPLLGNGVFTLDGEGWKESRQMLRPQFAR

DQIAHVQALEPHIQILAKHIRASKGKSFDIQELFFRFTVDTSTEFLFGESVHSLYDASIGLECDPEVAGFAEAFNETQKD

LAVRTYLQVLYFLYNPPSFWKNTKVVHKFAKRFVNKALNLSKDELEEHCKHHYTFLYELAKHTRDPKVLQDQLLNIMVAG

RDTTAGLLSFTFFELARNPEVWQKLKDEIYQHFGSGEDADVSKITFETLKQCTYLKWVLNEILRLYPSVPINFRTALKDT

TLPTGGGPDGTAPVFIGKGTTFAYSVYLLHRIEEYYGKDANEFRPERWADLKQLGWAYLPFNGGPRICLGQQFALTEASY

TICRLAQMFPNLASNYDQPDPCKKLIHLTMSLMDGAWIEMS

 

>CYP52A50 Lodderomyces elongisporus

71% to CYP52A37   Candida parapsilosis

LELG_03505

MMETIISNTGHALQPPYSSHTIWFGILFIIFGTYFICIFIKREIFRRAHHCKEPIIFPEGGPLGLRLGYLLQRLKTTGEL

VDFPMVLYEKFGRRTTFSAYLFGVRVVASVNPDNLKAVLATQFNDFGLGTRHAHFKPLLGNGVFTLDGEGWKESRQMLRP

QFAREQVAHVQTIEPHLQTLAKHIKTHNGGTFDIQELFYKLTVDTSTEFLFGESVHSLYDASIGLECDPEVAGFAEAFNE

TQKALAVRTYLQVLYFLYNPPSFKRNTKIVHKFAKRFVNRALCLTPKELEEHCKHNYTFLYQLAKHTRDPQVLQDQLLNI

MVAGRDTTAGLLSFTIFELARHQNVWEKLKKEIYQHFGSGEDALVNKITFETLKQCTYLKWVLNEILRLYPSVPINFRVA

TKDTTLPTGGGLDEKSPVFIGKGTTFAYSPYLLHRMPMHYGEDATVFRPERWADLKQLGWAYLPFNGGPRICLGQQFALT

EASYTICRLAQMFPDLKSMDDSPDPCKKLIHLTMSHMDGVWIQMSE

 

>CYP52A51 Lodderomyces elongisporus

75% to CYP52A35   Candida parapsilosis

LELG_03309

MLLDEFITYLNHWYVIIPLAFTTYHVFHYIYLNQLAKKFGAKPITNVLSDGWFGFKNGTQAIALKNKGQAVEWAQEKYYE

TKHPEIPTFKERIFGLYLVFTKDPENIKAMLATQFSDFSLGHRLAYFDPLLGKGIFTLDHEGWKDSRAMLRPQFAREQIA

HVKSLEPHIQYLFKHIDKNQNIQSKNKIENADYETSISASYFDIQELFFRFTVDSATEFLFGGSVSSLQDETIGCDTRDI

DFAGRRQFADCFNKAQNYLATRNLLQKLYWLVNPKEFRECNKVVHEFTNYYVNKVLSYSPEELEKISDGYVFLYELAKQT

RDPNVLRDQSLNILLAGRDTTAGLLSFVIFEMAKNPQMWTKLRNEILERFGTTNLEDITFENLKKCEYLKAVLNETLRLY

PSVPRNARVATRNTTLPRGGGPDGLSPIFIAKGSTVGYNISACQRDETHYGKDVDEFRPERWFEESTRKLGWAYLPFNGG

PRICLGQQFALTEASYVITRIAQTYSKLELKPGYGYPPKRMTHLTMCLFDGCWVKMERAQV

 

>CYP52A52 Lodderomyces elongisporus

70% to CYP52A5   Candida maltosa

LELG_00044

MSDLLPPIIQEYATKWYILLPAIVLVYLTFSFLFEIYLKRKLGAKGFTNTERDGYFGFYTPFLLLRLKKEGNLVDFGTER

YQFLQNPDVPTFKLRMFGIPIVTTKDPENIKAILATQFSDFLLGLRHAQFMPLLGDGIFTLDGQGWKDSRQMLRPQFARE

QISHVKLLEPHMQVFFKHVRKHKGMTFDIQELFFKLTVDSATEFLFGNSVECLKDSSIGMRPNSADFEGKEQFADAFNYS

QTYLSTRVVFQKFYWLLNGKKFKECNKIVHSFAQYYVNKALQLTPDDLQKQENYVFLYELAKNTRDPQVLQDQLLNIMVA

GRDTTAGLLSFVFFELARNQDVLAKLKEEIYGKFGFGADARVDEITFESLKGCEYLKAVLNECLRLYPSVPNNFRTALRD

TTLPRGGGKDGMSPILIRKGQNVIYSVYALHREEKFYGKDAAEFRPERWFEPETRKLGWAFVPFNGGPRICLGQQFALTE

ASYVTTRLIQEFEHLTMDPDTEYPFKKMSHLTMSVYGGVNVQMY

 

>CYP52C7 Lodderomyces elongisporus

54% to CYP52C6   Candida parapsilosis

48% to CYP52C3   Candida albicans

LELG_05768

MMIHLPLFIVLTLLLVLYNIIRFYRKQVIKRKYKCQKIPNAQTSTFLSYLGLEFIVNYNKIQEEGRIHQNMYKQFKDLKT

STLAQTMLFLKVVFTTDPENYRYMSSSANFNNWTIGDRPDAFDPLLGDGIFSSSGESWKHSRIMLRPFFSKDHIKQIEYM

EPYVKDVVRLINIYQRDGVVVDMQDIFQYFTIDYATKFLLGESCDTLKDALDEPTSVTIDPELKSRFAKAFDISLKYLML

RVSLGKPLMWLVNPKELREANKIQHDFVRYYINKALAMNEEELNKHSDNGNCFLYEIAKQTKDPKILQDEVMSIILAGRN

TTSSLLSFLFFELSRDANKEILKKLVDAVRSNFDSIESITYEKIHNCDYLRWCLYETLRFHPSVPLASKTAAVDTILPKG

GGLDNESPVFVPKGRRVVYPLYTANRNSKFFGSDTEVYRPERFADLPKSGGPAFMPFSTGPRLCLGQQLALTEASYITIR

LLQTFDIIEKVGDEPYPPRTSSSATMRLMDGAKVRMRTSSSTTP

 

>CYP56E5  Lodderomyces elongisporus

runs of EE probably in an intron seq

59% to CYP56E2   Candida albicans

LELG_03266

MIVLFITILLVLSALIVFPPLNFPRNIPTIPFYVSFLGGMDQLEKFEKYLRTKLEKHGAVKIYFASRWNILVTKPEYLVH

IFKNEDVFAKSGNHIKIPGSVLATYTGDNIISAHGQLWRLYRDVMAQSIQFPNLDPIAENTNNLMKDLENALKFDSSVAI

TDFLQQYSLANVGDCIVGSNLRPMHQKIKYIKLQIFKPFYMNFPSVEMLPIPSRIRAKQEVENFRQWFGDKLMDADHGAA

VKLATAYNEGKLTQKQYLDNCIILMVAGHENPLLLMLSLLYVLAKNPYMQDKIRCNKE

IEEHGKEEGGRVVEEEGEEGEEEEGEEEEEEEEEEEEEEEEK

SPELYLSAVIYETLRMYPPLNQIINRCTTKDTMLGNIRIPKGTYVGYYNMGTGRDKTVWE

SPDTFWPERWGNTIEEVHANYTRAKRLGHLPAFHGRKRACLGEKFALYQCKAMVQAILGKYKISLDEDWEERITPAGPVC

PMNMKLRFSKI

 

>CYP61A1 Lodderomyces elongisporus

84% to CYP61A2   C.albicans

LELG_05188

MSEFNVSTPLVDRLLNEQAKSFISSNQQINYLYNLYQTSSWLQIIATFIVSVLLYDQVRYQLNKGSIAGPKFKVWPIIGP

FLESLDPKFEEYKSKWDSGELSCVSIFHKFVVIASSRDLARKILSSPKYVKPCVVDVAIKILRPTNWVFLDGKAHTDYRR

SLNGLFSQKALEIYIGVQEKYMDIYLERFCKYEGPREFFPEFRELLCALSLRTFCGDYITEDQIALVADNYYKVTAALEL

VNFPIILPYTKTWYGKKIADETMKIFANCSAMAKVHIENGGSPTCIMDEWIFLMKEAREKHLDDPESRLLIREFSDKEIS

EVIFTFLFASQDASSSLACWLFQIVADRPDIVAKIREEQLRVRNNDPSVPLDLNLINEMTYTNNVVKESLRYRPPVLMVP

YVVKQPFPVTESYTAPKGSMIIPTLYPALHDPEVYDDPDTFIPERWETASGDMYKRNWLVFGTGPHVCLGKNYVLMLFTG

MLGKFVMNSDIVHHKTALSEEIKVFATIFPKDDLILEWHKRDPLAV

 

>CYP5217A6 Lodderomyces elongisporus

57% to CYP5217A3 Candida parapsilosis, yellow region is long (possible intron)

LELG_05700

MLLDILQATHSFVETHPYQTYVIVTGALLFYYLVFLPVLSPLWKVPGPYLHRISRIPSLHYQRKHSWGEKVYNLHKQYGN

IVILSPNEISVNGSDKFVNDIYLRNFPKSRFYANYTNHGDKDNIFATLTNDKHLKYKKMIVKLYSKSNVMAREGPLKRII

AETTQRLIDVVRKSSVTGETPDFLSIRPEFNPHAKGFTSPNWFNKLKRTQNLGIEVYTLFGCVAMDVVSRFELGEENGLD

LLLHPEERSIVAKHRQVAGMGFWTTLMPQFWEIAASKLVMNALKDITKFQLSLYADAE

ARLEKEDQPEMNENKRDNQQEKEFEELKGRRERKRS

NPTTLEALKKHGFFGNEAYSFLTDNLFAGHETTATMLCYFTYELSRPAHLHIQSRLKEELVNTFGK

PKGPTDIIEDLEKVDSLPFLDAVFTENTRVHASIPGAEPRVVDKKPYMVDDISIPPGTIISCLPFALHREASVFFDPNKF

LPERWLPHYGELEIEFKTRIRQQHKFMMPFGKGIRMCLGMNVAVIEMKMVIANLYWHFASRIDSDWCEEILQDDEPIKLG

KRHKGENKTDQEMMCMNDYYTITPVNEECWLRWFEQ