ࡱ> >@=  bjbjв 28к_к_8,4Wj:::::$w::T::PQqR'0W!!! W!X T: 8 Monosiga ovata P450s D. Nelson Feb. 20, 2008 Assembled from ESTs >CYP51A1 DC515864 Full length cDNA Library, Monosiga ovata Dec 18 2007 DC505021 DC481719 DC499362 DC515864 DC455628 DC461341 DC454243 DC488261 DC503517 DC463918 DC518896 DC496720 DC481661 DC475708 DC470881 DC457948 DC508866 DC473892 DC461250, DC476196 DC482539 DC489615 DC514865 DC490097 DC454294 DC469866 DC502657 DC513272 DC505282 DC458836 DC477187 DC482903 DC502887 DC495059(some errors) DC451008.1 EC165400 02-JUN-2006 cDNA Library, Monosiga ovata Dec 18 2007 54% to CYP51A1 Monosiga brevicollis MOC-079L11 3 prime DC505020 = 5 prime MQHIGSLTLADIGTRITEYVSTANRTHLLAGGVAALVTLNWIKK TYFRSSKLPPHAGSNFPFFGSMVSFGQHPVKFLERCYKEAGPVFTFTMLGSEVTYLAGGE VTDDFWSSKNDDLAAEDLYANLTVPVFGKGVAYDVPHPVFSEQKGITKKGLTQQRFAKYT AIIEKETLAYIQRWGESGTCDLFKDLSELIIFTATHCLHGEELRSTFDESVAALYCDLDK GFTTAAWFLPNWLPLPSFRVRDRAHRELIRRFTHAIRDRKAKG DVPGHDDMLETFMTATYEKVNDGRAFTESE TAGMLLALLLAGQHTSSTVSSWLGFYMAQNPALQQDLFEEQQRVMGSQSGPLSLEAINSMPHLWAA IRETLRLRPPLLTLMRNCRRPMEVKVGEKTYVIPKGNQVCVSPALQGVLEDLWDEPEKFD MNRFLKKDASGTEVVTDGTQVAKGGKLKWVPFGAGRHRCIGFDFAQVQIRAIWSVILRNY EITMTDVPEIDFTTLLQLPVHTKVHYRRRPTAAKA* >DC486766 Full length cDNA Library, Monosiga ovata Dec 18 2007 MOC-053F16 3 prime, DC486765 = 5 prime like Helicosporidium sp. CX129156 CX128716.1 CYP711 like fragment ~50% to DC460778 MSLTGIAAPLLPWQRSLVLAIATPILSIAVLLVSILYPSWRSPLRRALPSP PAPSLLLGHLPAMGKTGHSTLFAWAKKYGGAFFVRLGCWPAVIITDVELIKSICITQFKD FHDRSQAFRPNPHVRHLLWAQGAYWKACRNAVSPAFSRSNIAGFGAQMNQSARGLVDRVG ALAGTGQSVDIMRVVGAMTMEVIGGTALGVDLSASNREVSEKIIAAADLLFGSNVAGSTI TSFIRFLVPALLPLWLRVPALTA (34 aa gap) GFLQLMLAAKDPESGLQLTDKEVIEQCRLFMLAGFETTANTLTMAIYLLARNPDAEARMV AEIDELFTGTEVDYDSVQRFKYVDCVLQETLRMYPPGSNLLREATSDTVLGDLEVPKGTT IIMPMYTVHRDPALFPEPESFRPDRFLEGSALAPTDKYANLPFGAGPHMCIGNRFALAEA AVTLIHLYKNFTLRLAAPLPDPLPLRQSITLSPAVPIPVRFDRR* >DC460778, DC458147, DC494197, DC496896, DC457970 cDNA Library, Monosiga ovata Dec 18 2007 MOC-015K20 3 prime DC460777 = 5 prime ~53% to DC486766 MAVFSKLGISLTSIPALPRPSWAALAAAAPVVLTASAVAYMVLP GLLSPLRGQMPSPPTVSMLLGHLPAFTKRSHLVLLQWTKLLGKVFFVRLGAWPVVVITDI ELIKAVNISQFKDFPDRAPCFVARQDIRHLLWARGAYWKACRNAISPAFSRNNLTGFAQQ MNESAHALAARLGRAADAGEVINMMDVVGNMTLQVIAGTAFGVRLGPEAAGQTAAFVAAA KELFGSSVQGANLHGILRFIFPFLADIYTYIPPT (small gap at I-helix) YETTANALTF STYLLARNPAAEARLLAELADVGVPDYISYDDSQKYKYVECVVQEALRLYPAGNAIVRQA VCSTTLGPYEIPKDTCIVTPLYTLHRDPDLFPMPEEFCPDRFLDGHPLAPSDKYAHLPFG LGPHMCIGYRFALAEAVIALVRIYKDFTLRLDAAVPDPLPLQQGITLTADVPELFHVERRAP* >DC501006 DC513559 DC511491 Monosiga ovata Dec 18 2007 MOC-073O13 5' DC513560 = 3 prime DC511492.1 DC513560.1 36% to estExt_fgenesh2_pg.C_280122 Monosiga ovata ~45% to DC499869 MDILLTIFLSILGMLLFGLLCILAFCLPKPYEYL LSRKIINTKTGKPLSNNNIKLPFGDLLSCLKDVVGAERSRLKYIPENAIGWWALNNYDVQ LLNADWLRELLTMPDVKFNRSLESFKVLHRLLGTSLLGNQGEAWARMHKILYKAFQPSAL ASYVPFFISQTKEIANNIDASIATNTSYDMNLAFNDLTLAVMVDAGFGKAVSPADRKIIL HAFRYLFMETQNPLHDIPILSKLPFPSNLECERQFRALHETADRI (very small gap) MHASGEYQEAHDGKYVVDMLLDAHEEEGKLSDIELRDNVVMLLVAGSETTGTTLTWVLHY LTVYPDIKARVLAEIDTLDISWEHFNVTNFDTGMPYLTMVVSETLRDTPSIYGIPERIFT EDGTVGDLRLPKGSRVGVSQYCMHHNQNYWSEPDKFDPERFAPEASAARHRFAYLPFGLG RRQCMGKFFALNEIRVVIALLLKHYEFTYDASVGPVEVTWRPPTLMPKRGLPMFAKRR* Note: a short EST matched this sequence in the N-terminal region. It may be from a related P450 (from M. ovata TbestDB) >MNL00001922_unclassified N-term Genbank EC163715.1 Query: 63 CLKDVVGAERSRLKYIPENAIGWWALNNYDVQLLNADWLRELLTMPDV 110 C++DV G + SRLK E+ + W L Y +LLN DWLRELL M DV Sbjct: 1 CVEDVGGGDWSRLKQSCESVLDWRGLERYYCELLNDDWLRELLRMDDV 144 52% to DC501006 short sequence like N-term region in gray above CVEDVGGGDWSRLKQSCESVLDWRGLERYYCELLNDDWLRELLRMDDV >DC454381 Full length cDNA Library, Monosiga ovata Dec 18 2007 60% to DC499869.1, 46% to DC501006, MAVHDGHGVGSILALLGLGVLGVAAVGAACLVSFLLPSPAELRRRKALHNRITGNAL PTSGFHLNPLSDLLDELSDPFGTSTVRRDMAKGAQSGAYGLWAFGQYYAMLSNADWLREM LSMPDKAFRRSFQPFRTFSRLLDSSLIAAEGETWARQHRIMYKAFQPSALAGYVPLYARR AHMLADRLAAVAAAGGREDMLLAFNDLALGIMVDAGFGAAVSADDFKILFDCFRYIMCEM QNVVHDLPIIGALPLPTNVRLDHQFKALHAAA >DC499869 Full length cDNA Library, Monosiga ovata Dec 18 2007 61% to DC454381 DC499870 = 3 prime DC499870.1 35% to estExt_fgenesh2_pg.C_280122 Monosiga ovata 48% to DC511492.1 DC513560.1 ~45% to DC501006 MVVWSSPSWPATPASALRLAGLAVLGSVALCTACAISFCLPSLRSYIISRRVRVR STGALLPENQPGIPMKTILNEINDPIGENKRRLAAASGIGALGLWLLDSYSVLLANTDWI RELLSHPDKIYGRTFMPFKTMDRIIGGSLIGSEGDDWARQHRIMYKAFQPSALAGYVPLY ARRAHMLADRLAAVAAAGGREDMLLAFNDLALGIMVDAGFGAAVSADDYAIIFDGFRTAM QETTNIIHDLPIIGSLPLPTNIRLENQFKRL (gap) GISAVAPDVLTRVRAEVDAVVGSVEGMDSSKLDGVLPYLTKVVNESLRLNPPVAGIVGRI IKQDMTLGDITLPAGAGVGASTIMTHYNPANWDRPDEFDPERFGEEAVSKPPRFGYIPFG QGRRQSLGRVFALNEIRVVLAALLKQFPFEYDDAGGPVPLQVPPPSLVPPAGMPMTVRARL >DC514820 DC464533 Full length cDNA Library, Monosiga ovata Dec 18 2007 DC464532 = 5 prime end Best full length matches are to aromatase CYP19 low 20% range MLALASLLPTGLRSLYRKMLAMTELDPVDVQEDVLAHTVPQ VAHQLLEKRDVAVVKLGPRRVVFTCNPAIADHVFKEKQDCYIQRLCEDEGVRELGMYQQG VIWNNSAQWSSISKDVFHRAINPHALKEASRLARARAQLVFQSALQGQAAGGVDVLKCCR LVTLHVTLQLFFGISTEELSAQVSAESVIADVCNYFKAWEYFLLRPASSTPPDEKTQAHR DAINRLRTTVRIIIECARAKLAAAHADHS (gap) DGPPAPVATAPSASASASASAPPRASTFTVTRLLADLRRRRFEHMQQAVDTMPDDPEDRE AWCAWLAAAHDAAVEASAKSDLLKALLHESLRFKPVGPVVIRQAVADDILPASASPHTGT PMRKGDGIVIALDLMHRRADLFERPNVFDPGRFLQSTEDTDMIKFSQPSRFAPFGAGRKS CVGKDLGMAEILQVCA AILTAMAFDLGDNEPLADLETRWDIANQPTRPIALRACRPSLIL CGPSSSGKTTLRKQLQAEGGWSSHASIE >CYP710D1 Monosiga ovata (choanoflagellate, single celled ancestor to animals) GenEMBL CO435081, EC169877.1 EC166517.1 ESTs partial sequence 53% to XM_813889.1 Trypanosoma cruzi 710C1, 43% to 710A1 46% to 710A7, 56% to 710B1, 49% to 710A14 note: seq ortholog not found in Monosiga brevicola at JGI DC485544 = 5 prime end DC485545 DC517956 Full length cDNA Library, Monosiga ovata Dec 18 2007 MAQELLDAIAAWRPVATWQSALYATAALVSGYALYEQIRFRQWKQGMEGPA LAVPLIGSIVEMVKNPYAFWENQRLRNPCGISWNSICGQFMLFSTQTDVTKKIFMNNGED SFRLFLHPSGWKI LGEHNIAFKHGPSHKALRKSFLNLFTRKALGVYLGIQERLVREHLATWKLVDEPTEFRLK VRDLNLLTSQTVFIGPYLRSDEERVTFCNNYLLMTEGFLSFPIAFPGSGLWKAINARHAI VDKLVAAARESKARMAAGADPECLLDFWSQRILEEIAEAKPGDEPAEHWSDWEMGNTMMD FLFASQDASTASLTWTAAFMSERPDVLAKVQAEQKALRPNDEPLTYDMVEQLVYTRAVIK EILRFRPPAVMVPAIAMVDFPLTD TCVAPKGSL VVPSIWAACMQGFPHPEVFDPDRMGPERQEDVQYRDNFLTFGVGPHMCVGREYAINH LVAFLSLLSTTCSWTRIYTPESHTIKYLPTIYPGDCLIHLKPLQASA*     /01DEFGN/ 0 f g   : ; a b  E F q r   M N r s t   . / b c дhR{UCJOJQJaJhR{UB*CJOJQJaJphhR{UB*CJOJQJaJphhR{UhR{UCJaJ%hR{U5B* CJOJQJ\aJphG01EF0 g  ; b F r  N s t ed]ed]t  / c 2>{#$f"_[ed] 12=>z{"#$UVef!"^_Z[;<LMop&'TUef￿" *hR{UB*CJOJQJaJph#hR{UB*CJOJQJ^JaJphhR{UCJOJQJaJhR{UB*CJOJQJaJphK<Mp'UfYZG567ved]fXYZFG4567uvNOLMijz{,-ij  GHIJhR{UCJOJQJaJhR{UB*CJOJQJaJphZvOMj{-j HIMed]LM'(de(MN(TUkl#$(12no% & ( H I h38AjhR{UUhR{UhR{UCJOJQJaJhR{UB*CJOJQJaJphS(eNUl$2o& I ed] ed]gd38A h38AhR{U 01h/R / =!"#$% x666666666vvvvvvvvv666666>6666666666666666666666666666666666666666666666666hH66666666666666666666666666666666666666666666666666666666666666666p62&6FVfv2(&6FVfv&6FVfv&6FVfv&6FVfv&6FVfv&6FVfv8XV~ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@ 0@66666_HmH nH sH tH H`H Normal CJOJQJ_HaJmH sH tH DA D Default Paragraph FontRiR 0 Table Normal4 l4a (k ( 0No List D/D msonormaldd[$\$OJQJ8Z`8 0 Plain TextOJQJN/N 0Plain Text CharCJOJPJQJ^JaJ4@"4 38A0Header  H$B/1B 38A0 Header CharCJOJPJQJaJ4 @B4 38A0Footer  H$B/QB 38A0 Footer CharCJOJPJQJaJPK![Content_Types].xmlN0EH-J@%ǎǢ|ș$زULTB l,3;rØJB+$G]7O٭Vj\{cp/IDg6wZ0s=Dĵw %;r,qlEآyDQ"Q,=c8B,!gxMD&铁M./SAe^QשF½|SˌDإbj|E7C<bʼNpr8fnߧFrI.{1fVԅ$21(t}kJV1/ ÚQL×07#]fVIhcMZ6/Hߏ bW`Gv Ts'BCt!LQ#JxݴyJ] C:= ċ(tRQ;^e1/-/A_Y)^6(p[_&N}njzb\->;nVb*.7p]M|MMM# ud9c47=iV7̪~㦓ødfÕ 5j z'^9J{rJЃ3Ax| FU9…i3Q/B)LʾRPx)04N O'> agYeHj*kblC=hPW!alfpX OAXl:XVZbr Zy4Sw3?WӊhPxzSq]y d8  f t v @ @H 0(  0(  b S  ?C"]38AR{U@@@Unknown G.[x Times New Roman5^Symbol3. *Cx Arial?Z PTimesTimes7CourierC.  PLucida Grande9=  @ ConsolasC.,*{$ Calibri Light7.*{$ CalibriA$BCambria Math"1hӕgӕg , ,%0B@P $P'38A2! xxwL Normal8 Monosiga ovata P450sDavid R. NelsonMicrosoft Office User Oh+'0 $0 P \ ht|'8 Monosiga ovata P450sDavid R. NelsonNormalMicrosoft Office User2Microsoft Office Word@F#@qR@qR ՜.+,0 hp  'University of Tennessee,  8 Monosiga ovata P450s Title  !"#$%&'()*+,./012346789:;<?Root Entry FpRqRA1Table!WordDocument28SummaryInformation(-DocumentSummaryInformation85CompObjr  F Microsoft Word 97-2003 Document MSWordDocWord.Document.89q