Pichia stipitis P450s

 

D. Nelson

 

August 9, 2009

 

10 sequences + 1 related species Pichia anomala

 

>CYP51F1 Pichia stipitis

71% TO CYP51F1    Debaryomyces hansenii

JGI model e_gww1.7.1.351.1

MALLDSAIVAYNYFVSLSLTQQVLIIVFAPFIYNIVWQFLYSLRRDRAPLVFHWIPWFGSAIPFGMAPYKFFEDSRQKYG

DVFAFMLLGRTMTVYLGPKGHEFVFNSKLNETNAEDAYKHLTTPVFGKGVIYDCPNHRLMEQKKFAKYALTRDAFKTYVP

KIREEIISYFKNPEGFNMGEKNSGVANIMKTQPELTIFTASRSLLGDDMRKRFDASFAQLFSDLDKGFTPLNFVFTNLPL

PNYRKRDNAQQKISATYKSLIEKRRATGDVDPNRDLIDSLMTYSTYKDGVKMTDQEIANLLIGILMGGQHTSAATSAWFL

LHLGARPDLQDELFEEVSNILKEKGTSNLNDLSYEDLQNMTLTNNVIKETLRLHMPLHSIFRKVSKLLLVPNTNYTVPAG

HYVLVSPGYTQTSDRFFPNASTFDPHRWDSNNLADKNADEGTVDYGFGAVSKGVSSPYLPFGGGRHRCIGEQFAYVQLGT

ILSAFVYNVKWSLKGEKVPANDFTSMVVLPEEPAEIIWEKRKTCTF

 

>CYP52A53 Pichia stipitis

65% to CYP52A40   Candida guilliermondii

JGI model e_gww1.3.1.449.1

MSAELAFEYLTKWYSILIGAALIYGIARYIKIQLFIRKHGCEETPFLPDAKWFAIPIMSRVLKAKNEGRLVDLAQSFMTS

DRRTTHVYLGPARIIFTIDPENMKTMLATKFNDYALGFRHTHLAPLLGDGIFTLDGEGWKHSRSMLRPQFAREQVAHVRA

LEPHVQVLMKHIRLNKGKTFDLQELFFKLTLDTSTEFLFGESIYSLYDSSIGLTPPTDIQGRSEFADAFNTSQKYLGTRA

WLQFMYWVVQNREFYQCNAKVHKVAKYYVKRALNFTPDELEKASANGYTFLYELVKQTRDPVVLQDQLLNILVAGRDTTA

GLLSFTFFELARNPDVFEKLKNEIYEHFGKGDESRVEDITFESLKQCEYLKFVLNEALRMYPSVPLNFRVSTKDTVLPNG

GGKDGTKPVFVGKGTTVAYTVYCTHRDEKYYGKDANVFRPERWATLNKLGWAYLPFNGGPRICLGQQFALTEASYVIVRL

LQNFPNLVSKDDRPYPPAKSMHLTMCHQDGIFVELS

 

>CYP52A54 Pichia stipitis

63% to CYP52A44   Debaryomyces hansenii

JGI model e_gww1.3.1.459.1

MYEKTIIALLYLSKWYVLLGSAVAVLALYRYITVQLIIRKHGCKDPIKFTQGGFLCIPIILELLRRMKTGEMIDEGFRLF

NEYPDTTQYVNLFGVRVLLTGEPEVYKAVLATQFNNFALGFRHSHFAPLLGDGIFTLDGEGWKNSRAMLRPQFARQQVSH

VQMLEPHIQTLAKHIKARKGTTFDLQELFFRLTMDSATEFLFGESVRSLHDEVIGMQRPDIEGISNFSEAFNTSQRYLAV

RAYSQVLYWLTNPKEFRDCNAKVQKVAQYFVNKALSFSDSELEEKSKKGYIFLYELTKQTRDPKVLQDQLLNILIAGRDT

TAGLLSFTFYELARNPAIFEKLKAAIYADFGRGDVSEISFESLKKCEYLKFVLNEALRLYPSVPVNFRVATKDTVLPTGG

GPDGQSPMFVRKYTTVAYGVYFTHRMKQFYGEDAEVFKPERWAENRKLGWAYLPFNGGPRICLGQQFALTEASYVVVRLI

QMFPNIVSKDDGPYPPRKSVQLTMCLQDGVKIQMS

 

>CYP52A55 Pichia stipitis

63% to CYP52A14   Candida tropicalis

JGI model  fgenesh1_pm.C_chr_3.1000237

MANFIEFVTTNWYIIIPALLVLHKVFDLLYVQYLYRKLGAKPCTNQTDDHAFGIRAGFEMLKKKNEGTVVDFGAERFESR

IDPKIPTFSMRLFLIPIVLTRDPENIKALLATQFNEFVLGSRFEQLAPLLGKGIFTLDGEGWKHSRAMLRPQFAKEQVAH

VQSLEPHIQALAKHVRNAKGKLFDIQELFHRLTVDSATEFLFGQSVESLRDESVGMADEATDFAGKSTFAASFTIAQNWL

ANRAVAQKFYFLINPKEMRDSIKDVHRFVDYYVQVALDTPQDELDKKSKDGYIFLYELVKQTRDPYVLRSQLLNVLLAGR

DTTAGLLSFAFFELARRPDIWSKLKDEIYENFGLGENSKVDEITFESLKRCEYLKAFLNETLRLYPSVPVNFRVATKDTT

LPRGGGKDGSEPILVRKGQSVFYSVYATHRSEAYYGKDRHVFRPERWFEPSARKLGWAYLPFNGGPRICLGQQFALTEAS

YVVARLIQLFPNIENYEPEEVYPPFKNSQLTMNLLNGLHIGLY

 

>CYP52A56 Pichia stipitis

59% to CYP52A38   Candida parapsilosis

JGI model  fgenesh1_pm.C_chr_8.1000042

MFMTTDPENFKAMLATQFNDFSIGRRYQILSPVIGDSIFTLDGEGWKHSRAMLRPQFVREQVGHVQALEPHLQLLAKHIR

SYKGETVDLQQLFTKFTLDTATEFLFGQSVHTLYDERIGMKTPDDVPYAKDFTDGLFITQKYTSERGYAQQFYWLIDGKE

FRTAIANVHKFARFYVDRALNFSQAELEKKSQESYTFLYELVQQTRDPKVLQDQLLAIMLAGRDTTSSLLSFIFYELSRN

PGIWEKLKKEVYENFGSGTEKDIAKITFESLKKCNYVKWVINETLRMYPTVPVNLRVSNKDTLLPKGGGEDGKSPIFIPR

GTTVGFRVYSTQRNKEYYGEDPDVFRPERWADIGKLGWAYLPFLGGPRTCIGQQFALTEAGYILVRIAQLFPNLKSKNSV

HYPPKKTLNVIFNLFEGCLVEMGE

 

>CYP52A57 Pichia stipitis

62% to CYP52A39   Candida guilliermondii

JGI model  e_gww1.3.1.1392.1

MTVETIVQYLLCWQVYVLAVLVYHIFNWIHEQILYKKFGASPCINNERGGFLGIRLLRTLLKAKLDGTLSDVVKNRYYEM

EHPEIETFTLRVFSQTVIATRDPANLKAILATQFNDFSLGIRHAQLYPLLGDGIFTLDGEGWKHSRAMLRPQFAKEQIAH

VQSLEPHIQVLAKHIRKSEGKSFEMQELFFRLTLDCATEFLFGESVESLRDASIGMARADVEVEGKDRFAEAFNLAQTQI

ATRSMMNKMYFLYNTEELRNSCDVVHRFTDYYVNLALRTSTAELDKKSKSGYTFLYELVKQTRNPQILRDQLLNILLAGR

DTTAGLLSFAFFELARQPHIWAKLKDEIYSAFGSGENSRIDEITFESLKRCEYLKAVLNETLRMYPSVPNNGRIAVRNTT

LPRGGGPTGTEPMLVRKGQKVVYSVYTTHRSKTHYGEDAEVFRPERWFEPSSRKLGWAYLPFNGGPRICLGQQFALTEAS

YVVTRLIQMFPNISSDPTIEYPPRKASQLTMCLQDGLLISLY

 

>CYP56E6 Pichia stipitis

57% to CYP56E4   Candida parapsilosis

JGI model e_gwh1.2.1.1913.1

MISALIVGVLLIVKSIILPPSNFPKSIPTIPFYVSFLGAYTSMDQEEIYNYYMREKLEKYGAAKLYFASRWNIVIIEPEL

LLQLFKNENIYAKSGNQEKIPYSVLAQYTGENIISAHGEKWRLYRRVVASSVQFPNMEPIVENSKRFLDVLHQELINNSP

IEIIDLLQRYTLANIGDSVLGVKFNTMEEKDTEIHKRIKHVKRQIFQPLYMNFPFLDKLPIASRMEARKEVAQFRSYFSN

ILKTCQNFGRETGASRLRQALDNNTLTEKEFTDNGIILMVAGHENPLLLLLSLFYIVAKYPEVQHKIRQENGDNEAPYLN

SVICECLRMLPPLGQIINRRTTENVILGSNIRIPKGTYVGYNNFGTGRHRKIWGESADEFKPERWGKTPQEISRKYLEAK

IKANLPAFHGRKRACLGEKFALEEVRVLIQTLLQMYQLLLDPNWVEKITPAGPICPLGLKIKFKKIEF

 

>CYP61A1 Pichia stipitis

82% to CYP61A1   Debaryomyces hansenii

JGI model estExt_genewise1_human.C_chr_6.10410

MSFNTTSPLAVGYKNAQQYLDIETFITKAQETYSSLTWIQLIFSVIAIILSYDQIMYQIRKGSIAGPRFKIWPIIGPFLE

SLDPKFEEYKAKWDSGELSCVSIFHKFVVIASSRDLARKILASPKYVKPCVVDVAIKILRPTNWVFLDGKQHTDYRRSLN

GLFSQKALEIYIPVQEKYMDIYLDKFTKFDGPREFFPEFRELLCALSLRTFCGDYITEEQIALIADNYYRITAALELVNF

PIIIPYTKTWYGKKIADDTMKIFEKCAAMAKVHINEEAGKPACVMDEWIYLMKDAREKHSEDPEAKLLIREFSNKEISEA

IFTFLFASQDASSSLACWLFQIVADRPDVVEKIREEQLAVRNNDPSVPLSLDLINEMSYTNNVVKESLRYRPPVLMVPYV

VKQSFPVTETYTAPKGSMIIPTLYPALHDPEVYEDPDTFIPERWENATGDMYKRNWLVFGTGPHVCLGKNYVLMLFTGML

GKFVMNADLIHHKTALSEEIKVFATIFPKDDLIMEWKSRNPLEA

 

>CYP501D2 Pichia stipitis

56% to CYP501D1   Debaryomyces hansenii

JGI model e_gwh1.7.1.272.1

ILGVFKIPGYPIVGNLFQVINNPAQVFMKWSEVFNVSLFQIRFGSRSVVVVNSFEDVTRLWIKYSCSNNSRPVLYTFHQV

VSLTRGFTVGSTPAGESYKRKRKSISQHLNHRAVERSKSTLDKEVEYMINQMISRNHELSGPPSVNMFRNSKCELADIDM

KIYFQQFALRSSVFFAFGIHLDCFGKDSKLCNEIIEVESAIMKFRSPIANLEDFLPILRYFPGKFKKAAEFRERRDRYMN

KLYKTMIEGIVVGNADAVASIVGTIKIEQDQQMNKLTEEEIQSICLTLVSAGLDNTALNINHLMGHFSQPFYGAALQKKA

YDALLAGSNGDICQAWDDAVDVKCPYVIALVQESLRYFTVLPLGLPRITTKDIVYNGAFIPKETILIMNAFAANHDSCVF

QSPYEFIPERWLDAETGELIDKHDLIHLSFGAGSRMCAGIQLAINEMYTFTSRLVLYFKIKPPTCGAIMDLDPFRNNENP

SATSFEPKPFKVRLEPRYSDSLYNKLRRS

 

>CYP5217A7 Pichia stipitis

57% to CYP5217A2    Candida lusitaniae

JGI model e_gww1.7.1.292.1

ILGVFLAYHLILYPFFLSPLRDVPGPYLHRISSLSSLNKQRKGRWTMTVYELHKRYGDVVVLSPTEVSVNGNSKYVHDIY

IKNFPKAKFYENFRNHGFKDNMFASLENDRHIAYKKLIMNLYSKSAIFNKKNGTHSIIVQKVGQLVDDIRQSSSGKTQNL

GIEVYTLFASLAMDVVSAFELGMSNGTDLLLHPEKRHIIISHRQVASMVFWTTLMPRFWNWAASSTILKALYDITKWQLG

LYAQAEENVPDVKEDENKSTLETLKQKGLKGEYAYSFLTDNIFAGHETTAIQLAYMCYELSRPKNLHLQESLRKELIREF

GHPKSEKDIIDDMSKVDVLPYLNSVLDENSRVHASIPGSEPRLVDSPYKVEVGEGKVVELPKGTGISCVVYALHRQSDVF

AEPDQFIPERWLQYENEPDEVFRQRIKRMQRYMMPFGKGIRMCLGMNLALIEMKMAIANLYWHYNSKICSDWCEITEYTE

DSKKLNPILMGETRKGENRTDEEKMTMYDSYTTRPLNDECWLEWYDNQFEN

 

Related species

 

>CYP51F1 Pichia anomala AF019903

EFVKGALTRDAFKSYVPQIREEIIHYFQNSKNFKFGERKHGISN

VMATQPEITIFTASRSLMGKQMRERFDESFAGYYSDLDKGFTPINFVFPNLPLPSYRR

RDYAQQKISSTYMGLIKERRENNDIKDRDLIDTLMKTSTYKDGVKMTDQEIANLLIGV

LMGGQHTSAATSAWFLLHLAEKPELQEEIYQEIMKVTGGEDVTYDDLQEMPLVNNVIK

ETLRMHMPLHSIFRKVKNPLPVPGTSYVVPKGHHVLVSPGFAMTNENYFPQPEVYNPH

RWDEDSTSKAAQDSSTVDYGFGAVSKGVSSPYLP