Puccinia graminis f. sp. tritici P450s

 

D. Nelson

 

August 9, 2009

 

17 sequences

 

>CYP51F1 Puccinia graminis f. sp. Tritici

55% to CYP51F1   Malassezia globosa with a short seq gap.

PGTG_07202 revised

MSSLIDPLIEFIGSFSTFNQILIYFLLSITSIISINIFNQLAIPKDPTTPPVVFHLFPFIGSAVSYGIDPYAFLESCRKK

YGNVFTFVLLNKKVTVALGLEGNALILNGKLSQVNAEEAYTAL (1)

TATPVFGTKMVYDVPNAILMXXXXXXXXXXXXX

NFRKYVTLIADETISYLEDHVFE (1)

KPKTQQAVKDCFKVASEITICTAS

ATLQGPEVREGLNKSFANLYHDLDGGFTPLHFAFPNLPLPSYRRRDRAQVAMRNFYMNIIQKRREDNREGQLGDMIDSLQ

GQTYKDGRPLTDKEIAHIMIALLMAGQHTSAATGSWLLLHLASRPDIVAELRQEQIEVFGKPGQTDDKELDPLDLERVQS

PLMLACIKEVLRLHPPIHSIMRKVKSPITVPRTLASHNEDTPYIIPSSNFVLAAPGASQIDPAIWSSPHEFEPSRWLKLT

SPFKAGGGETQEEMVDYGFGMISSGANSPFLPFGAGRHRCIGEQFAYLQLSTLGATVIRNCELELVSNQFPKPDYTTMLV

CPIKPRDVKFTRRNTHS

 

>CYP53B3 Puccinia graminis f. sp. Tritici

54% to CYP53B2 from LNRYP

PGTG_08085 +  PGTG_08086 revised

MLVIGLIAAFLEYSILLGLVGISCYYLTGYLRNKHQLNRYPGPFLAKFSRLWLGYATRFGNRYQIIHQLHQKHGRFVRIA

PNELSIADPDAVHIVLGHGTGTTKSKF (1)

YDAFVAIHRGLF (0)

NTRDRADHTRKRKIISSTFSQKSILEFEPYIADTLACFLRKIDQVASEP

NLVQLPSHSWDSKHLNERWRIIDILPWFNYLAFDIIGDLAFGERFGMIERGADIAAVEKEGKVIYLPAIQILNERGEFSA

TQGQFDHIKFSKKKKEKKK (0)

YIDPWFSRGAASVQNLTG (0)

IATNQV

NLRISQTGQSRRDLLARLQTGQDADGNPMGKDELIAEALTQLIAGSDTTSNSSCAILWWVVKHPEVHKRLMEELDE

HLGTEEGVISYADCKELKYLNACINETLRIHSTSSIGLPRILPQTVSFKGHILPKGLVCSVPTFEIHHDPDVWGDP (2)

FTFRPERWLEPNAKDREKAFMPFS (2)

CGPRSCIGRNLAMMELY (0)

MITSTIFKRYEFALVDPDLAELETREGFLRKVGFSLQQKT*

 

>CYP67A2 Puccinia graminis f. sp. tritici

76% to CYP67A1   Uromyces fabae

PGTG_03841 revised

MNYPASMNEFTSATFFSFLVIFPT (0

VAHLIFRNHEPTAIHYLCSQAVLFLLLEIGFQNSFFTGLLTLSAFNLSLAAQIAFYRLFWHDLR

SFPGPKLAALTQAWILREAYLGRTRFTMKELGEKYGEWVRI (1)

GPNELYTTSIEALYTIMGAKGWPKGPSYDSGITKGDLGGDSVLTIKS (1)

LPEHATRRRI ()

WNKAFTPKAIAEYLPSIENRLDEMIGVIDDHVKQKRNVDLCLQLGCFVYN (2)

TMCDMAFG (1)

APAGTGLLKNQGEKHRILTHMGRVVRQVGIVRNMPWLTP

LVKAWPSTQRKEQSEFRDFTKSMFLRRRNQGFGSQLDVFHYL (0)

LGEDTETGTRLTAAELAADSTLLVI (2)

TGSDTTRTVFLVFFLYMLKHPSYMEELQAELIAAPDLSPPTLARLPYLNACLQETMRM

QPPSPANLQRMCPPGGAVICGRHIPEGTKVRFSNYAIQRDERYFHDPDEFRPQRWL

RNAEEKLREGEDTERLNEKAFFSFLIGPGACVAKNLAWMEMRL (0)

VVATLLTSYDVAFAKGFDPVAFEASWTDAYLLLIEDPFEVTFTPKAGRLR

 

>CYP5152B1 Puccinia graminis f. sp. tritici

45% TO CYP5152A2

PGTG_14706

MMELIPATMRLIHNEIVQNFRIIFSLFICILSPVILSKITHRRRRLPLPPGPKSYPFIGYSFGPYHWRQMEKITKEYGPV

SSVRLGNNKLLVIVGRVEPALALLEGRSSIYCDRPQLEMAGNIMSGGLRTLCLPYGDRWKRFRR (2)

VLHSQLDTKAATSF (0)

YQPIQERASRQLILDI

LERPKDFTEALTRYAASVIIKITYGKLTPIHHSDDEVVQVIKTLTRFSKAARIGAHAVDRFPWLRYIPGWVAQGRKWHQE

ELNLFSSQVDGVRREMNIPGKRDSCFASYMLERQKEFSLSDNEAAYLAGSLFGAGSDTTAVMVLAAALAIVIFAAACHPE

EVKKVQEELGSVVGNGRMPTFDDYFELPLVTAFVCETFRWRPASAAGFMHATVKDDVYEGHFIPAGSWVVGNHWSIHRDE

SVFPDPDKFDLMRWLTIDDKTGKTVLRQDMRHFAYGFGRRRCAGITIADRTFEDATNSRPKPFEVDFVPRVPDLRRAIEE

MSAC

 

>CYP5221B1 Puccinia graminis f. sp. Tritici

42% to CYP5221A1, 40% to CYP5025A1   Ustilago maydis

PGTG_09055 model is short, THIS FAMILY HAS THREE MICRO EXONS

MITFLFANLLAYSLVYLLIKL (2)

LIIVFF (1)

SIGTKKRNDPSFSEIPGWPL (2)

IGA (0)

LPAVMKNRARVLEFLTNNGLK

YGLGFSFTLPGLRVVDISKPEWIEYIQK (1)

TNFENYVK (0)

SLLFQPMWDVFGQGIFMTDGTVWKRSRHAISTIFTNKTFKNIIEPHTEKSLD

GLTKELQAAAEENGSIDFCHLFHRFTLDSFVGMT (2)

FGKDLGIVGTEYSDQSGLGPAK

ATYSATSFVKAFDFAQDQMDFRMAVLSGWKLMEALNGSGEQMKRSCRVIDDFAYSLID

ERMSQSSHKVSSEDEESSSKDLLALFMNARDERGGGLGRIELRDTAMNLIFAGR

DTTAQTLSWAFFHLLMNKGLISKVREEGNDILGEGEGSRRGVTYANHKK

FLWSHAVVLETLRLHPSVPK (0)

NGKIALSDDKIPGGPLIQAGDMVRWS (2)

DWKMGRDAGIWGPDCAEFKPDRWIDEKGSMKQFGQFKFHAFN

GGPRICLGMNLAIFEAVKVIVEVFRSFELEFAAGWLENVPKS

EFIEGIAGSYRVPKYKASITLPMENPMMISVRNYGA

 

>CYP5221B2 Puccinia graminis f. sp. Tritici

63% to CYP5221B1

PGTG_09702

MITFIFANLLACSVVFLLIKL (2)

LINS (1)

SIGTTKRKDTGFYDVPGWPL (2)

LGQ (0)

LPSILKNRARNLEELTLRGLRYGPGHS

TTVPGIRIVDVSKPEWIEYIQK (1)

TNFSNYIK (0)

GPLSRALAYDVLGDSIFVSDGPVWKRARHATSTIFTMKTFKTTIVPCANQSI

DRLVKVLKSTAEVNQSIDFCNLFYCYTLESFVQMT (2)

FGTDLGLLGIEYNDEEKARSPSKLSQTTIP

FAKAFEFAQDQLDFR

IAMVMGWQMMERLNASMGNRMKASCCVLDEYVYSLIDERMAKMAQISEFEDKESSHSDLLSVFMNARDERGGGLGRTELR

DTTLSLIVAGRDTTAHTLSWAFFHVLMNKDLVSKIREEAIEILGDQDSDQDRVTYENYKQFIWSQAVVYEALRLHPSIPK

SGRYVASDDRIPGGPTVEAGNLVRWRFAWKMGRDASLWGPDCGEFKPDRWIDETGRIKKFGPFKFPAFGGGPRICIGQNL

AMLQAVKVIVEVFKQFDLEFAPGWLENVPKSEAIEGVASRYPTPMYRPSLTLPMAHPMMVSVHRRTSSAEAISP

 

>CYP5221B3 Puccinia graminis f. sp. Tritici

69% TO CYP5221B2

PGTG_04658

MITFLFANLLAYSLVYLLIKL (2)

LIIVFF (1)

SIGTTKRKDTAFYEVPGWPL (2)

IGQ (0)

LPMMFKNRTRNLEDNTVRALEHGPGFSITVPGIRIVDISKPEWIEHMQKTNFENYVKGPLLRGIMYDVFGDGIFVAD

GPAWKRARQATSTIFTIKTFKNIIVPAANKSLDGLVDLLKSTAQSNDQSIDFCDLFFRYTLDSFVQMTFGQDLDLFGTQY

DGKTKGSSPSKLSASSLPFSEAFDFAQDQVDFRFSVVIGWKLLEGLVGSIGKRMKASCRVLDDYAYSLIDERMASRTYQS

NLEKNEAVDGDLLGLFMNARDERGGGLGRTELRDTTLNLIIAGRDTTAEALSWAFFHLLMNED LISKIREEAIEILGDEN

DGQERVTHENYKRFVWTNAIVLETLRLHPSVPKNTKCIVSHDQIPGGPTVEAGDIIRWSGWQMGRDASLWGPDCGEFKPA

RWVDEKGSIKQFGPYKFNAFNGGPRICLGMNLAMLQAVKVIVELFRHFELEFAPGWLEKVPKSEAIEGITSRYPTPMYRS

SLTLPMDNPMMVSVKCKA

 

>CYP5221B4 Puccinia graminis f. sp. Tritici

51% to CYP5221B3, 52% to CYP5221B1, gc boundary at KKIK

PGTG_07113 revised

MGLHLYYRLFWGTNRCFFFSCLGTKKIK (2)

GELD (1)

SIGTSKRKDSKFADIPGWPM (2)

IGQ(0)

IPEVISNFSHPLELGMTRHLTFRPGWSVT

MPGLRLVDISKPEWLEYIQK

TNFHNYVK

GSRFHSVMTDVFGNGIFVTDGQLWKNSRHILAPLFTVKSFKACISPSLRVNL

DTLIEGLELASESRPTVDLCDVLFKFTLNFIVYTTLGKNMGQLDQLHLPPIRPLSPSPDSTFVDAFDFAQNQLDFRFSII

TLWKVFERVNIRLGNKIRSACKTLHRYASSLIDERLASFDQDAQRYHETPTDLLGFSLKIRKEMGGELSREELKDAFLNLIIAGR

DSTGEALTWAFYHLLMNED

LVAKIREEASTITGKDRAGQ

VTYENNKEFKWAHAVVLETLRLHPSIPKNIRFALKADKIPGGPVIEAGDAVRWSDWAIARDPEVWGEDCLEFRP

ARWIDENGNIKQFSQFKFHAFNGGPRVCLGMNFAIFQCVCLIVEVFHNFELEFAPGCR

 

>CYP5221B5 Puccinia graminis f. sp. Tritici

55% to CYP5221B3

PGTG_07103 revised

MLTSATLQSLLLGGFAYLAYLLFEF (2)

RDR (1)

SIGTTKRKDPLFQDVPGWPL

IGQLAQSLADLSRPLEASTLMALKLRPGFSIT

VPGVRIIDVSKPEWLEYIQKTNFDNYQKGPMFRGLMVDLFGDGILVTDGALWKRARTVTSRVFSANTFKNVIEPSADHSV

DGLLQVLQSSSDESRDLDFCNLFTRFTLDSFVKMTFGRALGLYGEESTTDTEPEACYKHLSSPEAFAEAFEFSQKQMDFR

FTVMTGWELYEKVNLSVGQQMKRSCGTIHEYAYTLIDERLSKISSDDDFTNGETFQNDFLGLMMAVHRQRGHTLNREELK

DAALSFLLAGRDATAQSLSWCFFHLLMNKEVISKIREEAAQLLGTYPSHQGRVTHDNYKQFTYTYAAVLETIRLHPPVPK

NLKFAKADDLIPGGPTVEAGDCVTWSDWQMARDPEIWGADCGQYKPDRWIDEAGKIQNFGNFKFHAFNAGPRLCLGMNMA

IFFNVKTIVEMLQNFDLEFSEGWLENVPKSEVLEGIKADYPTPQYQPSLTLPMKNAMMISVKPRLTQDLTILPIEHLYAC

TK

 

>CYP5221B6 Puccinia graminis f. sp. Tritici

60% to CYP5221B2

PGTG_09701 revised at heme signature

MSSYVSGKLVVCGLLYLIYLLIKYRDRAIGTSKRKDPSFTELPGWPLLGQLPNMLKSRSRILEASTMTSLKVGPGFSFTI

PGNRVIDISKPEWLEYVQKTNVKNYAKGPLRKDIMLDVLGRGIFVVDDALWKRARHATVSIFTPSTFKTIISPSVNQSID

GLTQVLCSAHKENRTVDICDLFFRFTLDSFIRMTFGKDLGILGAEYGSHSESSAPEFARAFDVAQDQLDFRLAMVVGWEL

IERLNVRSMGQRMKESCRILDEFVYSLIDERLANLSHKSDFQDQDPSHPDLLSLFITTRDERGGGLTRTELRDTAMNLII

AGRDTTAQALSWAFFHLLMNKELVSKIRKETIEVLGGDQESVTYDNYKRFVWPQAVLHETLRLHPSVPKNAIVALSDDKI

PGGPTIEAGNLIRWSDWQMGRDPSVWGADCGEFKPERWIDEAGSIRQFGQFKFHAFN (0)

GGPRVCLGINLAIFEAVKVMVEVLRDFELEFAEGWLENVPKSVTIDG

LTSQYQTPMYKSSITLPMENPMMIAVKRR

 

 

>CYP5221B7 Puccinia graminis f. sp. Tritici

64% to CYP5221B5

PGTG_07100 model is too long at N-term delete part of it.

Added I-helix region

MLTSPVLHSLIISGFAYLTYLIFEF (2)

RDR (1)

AFGTTKRKDPLFKEVAGWPL (2)

LGQLPGSILEIARPWEAGTIMTLKLRP

GFSITLPGARVIEISKPEWIEYVQKTNFENYVKGDMFQSLMADLFGKSILVTDGAAWKRSRLVTSRIFHINTFKIVVEPV

VDQSMHEVLEVLQVAGDEGRDIDFCNLFNRFTLDLFAEMTFGTKLGLLEGNSAVKDEGGEGYSYPEAFCDAFNFSQKHMD

SRFDVAVVWQWIQKMNFRATKKMESSCHTIHDHAYTAIDEKMSKLSTGDLTADDTLQKDFLGLIMLSHLQKGHTLTRDEL

RDDLLSLLFAGR

DATAQSLSWCFFHLLMNKDIITR

IREEAAEILGEDSENVGRVTPDNYRLFTCAYASLLEAFRLHPAVPKNVKFAKANDKIPEGPMIEAGDC

LTWSDWQLARDPEVWGPDCGQFIPDRWIDETGNITHFGNFKFHSFNGGPRLCVGMNMAIYIEVKTIVETLQKFDLEFSQG

WLENVPMSEEIPGVKTLYPTPQYQPSLTLPMKNPMMISATPRKL

 

>CYP5221C1 Puccinia graminis f. sp. Tritici

46% to CYP5221B1

PGTG_09163

MSELSSHLTVVLLVLVAAKLLSLLIK (2)

KSGA (1)

CFGTSKRQDVYDDLPGWPL (2)

    LGQ (0)

(0) LPEVILNSRNLLEWAAQKTLIHGVGYS (0)

VTMPGLRLIEITRPDWIEHVQK (1)

TNFQNYVK (0)

GSLFQEVMSDVFGQGIFVTDGAAWKTTRQTTARIFNTNNFN (0)

NIITPAVHKTLDSFMEVLNFHSETQTAVEMDSLLHQFTLESFVKMT (2)

QDMGSLKAGLSGQKTEPFAEAFDYVQKQLDLR

FIMTAIWVRLGRFVGNRPKMIAARRTLENYAYELID

SRAANPNKDTQVYQDLLGLFMSFTDEKGLSLSRSELKDSALNLIIAGR (2)

DTTAQALSWTFFHLIRNPDVVAKMRAEIDQLMASNDELVDYLNYKQFT

YNLAVFYEALRLHPSVPK (0)

NAKFAVDHDKIPNGPLVQPGDCLRWS (2)

DWQMARDPSIWGPDCTEFKPSRWIDDSGNLKQFGQWKFHAFN (0)

GGPRVCIGMHLGTLEAIACLVQVVRTFDLEYEPGW (2)

YEKVPMIRKISPDSTEQTPRYAASLTLPMLNHMRILVHKRKS*

 

>CYP5230A1 Puccinia graminis f. sp. tritici

26% to CYP526F1, N-term seems long

PGTG_03316

MSSVSAMSSAEFPFLSLHLLSHSFSGIFFTHRFDFGNLILGEHSTTNSWIQKNAVLVLRRWTEWFTGPHGFYIISFFILG

LLIYDALRPRPLNGIPTSGRYCWALGDSGALYRHMKLNKTRTTFFTDQANRVGPLSQVMLGPAGSWFGRLTGLGSHIVVL

ADGQQLVEVCAKRSREFPDPGLTLDI (2)

YKGIIPNGQLALPTGPTFKHHRRA (2)

IAHSMSSSHLSQVTPKITNSVIELIQL (1)

WKKRSQILDRSGEKYFKAAQDLRLSTM (0)

DTISDIIFGKPFGVMSARLAHLENSNGALSADARPQFPTLARALNVIVAQVARCYISPSKSLFWFMQRLF

NREWRQAKSTVFAYLKNEVEKERITLSEEQSFGGVDSKDPDNVNSVLSLLVKDEHLSKLRGEKPLST (0)

DEITQELLVYWVAGH (1)

GTMACTLAWAVKLLSNNPEVQYKLRDQLVNTLPSLNERSPTFSDLKVCSSDLGYLDATCFEILR

CGKVLGEVSRTSRVDTMVMGHVIPKDTVVMMPHSRLSGPQQSESEFRPERWI

DCDGKFDAQLPGPLHVFGHGQRGCFGKNLA (0)

VLELKLYIAMLCMELFFDRVPDDVNSMDAIELVANHPQTCVIRPIPWSKISNDPTKHL

 

>CYP5230A2 Puccinia graminis f. sp. Tritici

97% to CYP5230A1

PGTG_13050 model is short, revised

MSSVSAMSSAEFPFLSLHLLSHSFSGIFFTHRFDFENLILGEHSTTNSWIQKNAVLVLRR

WTEWFTGPHGFYIISFFILGLLIYDALRPRPLNGIPTSGRYCWALGDSGALYRHMKLNKT

RTTFFTDQANRVGPLSQVMLGPAGSWFGRLTGLGSHIVVLADGQQLVEVCAKRSREFPDPGLTLDI (2)

YKGIIPNGQLALPTGPAFKHHRRA (2)

IAHSMSSSHLSQVTPKITNSVIELIQL (1)

WKKRSQILDESGEKYFKAAQDLRLSTM (0)

MDTISDIIFGKPFGVMSARLVHLENSNGALSADARPQFPTLARALNVIVAEVARCYLSPSKSLFWFIQRLFNREWRQAKS

TVFGYLKNEVEKERITLSEEQSFGGADSKDPDNVDSVLSLLVKDEHLSKLRGEKPLSTDEITQELLVYWVAGHGTMACTL

AWAVKLLSNNPEVQYKLRDQLVNTLPSLNERSPTFSDLKVCSSDLGYLDATCFEILRCGKVLGEVSRTSRVDTMVMGHVI

PKDTVVMMPHSRLSSPQESESEFRPERWIDGDGKFDAQLPGPLHVFGHGQRGCFGKNLAVLELKLYIAMLCMELFFDRVP

DDVNSMDAIELVANHPQTCVIRPIPWSKISNDSTQHL

 

>CYP5231A1 Puccinia graminis f. sp. Tritici

38% to CYP5093A4 Phaeosphaeria nodorum, 37% to CYP5065A1 Nectria haematococca

PGTG_16636

MLSISENITILISIAILTLYLQACYSNRNKKIRNHHHHQTDKKTIKKEIPQPTGWPWIGNLYQIRRPNPWIQMARWTKQF

GPIYRLKMGSSDLIVLGSPKAAVDLLEQRSSKYSSRPRNIMTSEYVSKGLRLTFMPYNDLWRRQRKLLHLLTQPKAAANY

QPIQAQESAQLCLDILNHPNDHWKHSQRYAGSTVLQIAFNRRALSFQDPAITKMRECNEKMIETAVPGRYLIDSIPILSY

LPAILSPWKRYGNRLFNETLALFGQLYRDVLRSESPHPNLAPSDGACFVARIESLKESYRLTDDQAIFLAGAMFGAGSDT

TADAIETFIFACAANPEKVAKAQEELDRLVGRERLPEFSDEDGLVYCGAMVRELLRWRTVIPGGLAHMSTEDDEYEGYFI

PKGTTIVANHWAIHLDEETYKDPSKFIPERFIDPQTGSLIGTKSSVYGHHAFGFGRRICPALHIANKSLFITFTRLLWSF

EIKIKESSLLSPEEFVRAVEFSTGFSSHPIDLNDSLIEILPRDRSSLAVLTEAVENAGIDPIKL

 

>CYP5232A1 Puccinia graminis f. sp. Tritici

very poor match to other sequences

PGTG_08206, seems long in the middle region

MSSSSESYLESDPTVSSKTFSLTLRPQLFFAKAQHAVAKKSQQRALSCFTGSWDITSRFKTEHNSSTNFWKYSPRIWTWP

WIGSLCSYTRDPLGFIQTQHRSQGDVFTTTILGYNCTFIQSNEYIQSFATATRKVLDVTAAYKLIAAPLIGEEAFFGQTK

YMHQVVLSKSRTELLHQRLWELAEELLEIKWAQCFSSGDQTEVRNWQKIDLGQMLDYVIFGLDVFFLIGEEMVNVNLDRI

SHLFRVMDSDLSLLGILLPMIRGGRCPRKEAKNELLGFLKRDVQRRIERHVQHAVNSAFGSSQSDCAETWPDGPEEDSQE

LPDILLRSELKELDEAILFDYHRGESSAIKLINEKVEFIAIFIYGFVWAAQTNSAAATIGLVHDILLHDQSQALSDEHSP

CFSHAEQIRRECASAVVKDTPSPAPFLEDMPHLAHCLNETLRLRATGAWVRLADKSFHLSPQGNQTSGVICPPGFIVLSP

MPVSLNPSTYPNPSRWDPYRYERAPFISSPSGSNKDAARHIPIDKQYLVPPTTPSSPYFASWGLGQGHCPGKHLAYKMIS

MTVARFFDKFEVRPTKEYFENANFEDVAVAGVQRLKNEFTVLILSITRSKFHWVYQKEVHKTGMSGIARVYGKEAVVVLI

AATSLCLKHSRRLNINLVCEDENVGQWKLFEGQSKPQLLVRVKLVIRHASNPVIANWGAGSEDLFLQTNLQRGVR

 

>CYP5233A1 Puccinia graminis f. sp. Tritici

35% to CYP63A2   Phanerochaete chrysosporium

VSKKFSFYMLFAEFMVVLIPRVTMITQTK removes as a predicted intron

PGTG_08907

MSRVALLLSFVGPLLLSKFLLESFERNPSFLDRLVVFCLSWPIAREIRLRLEQRDLRARASSRGALLAPLVSSPLPFGLS

VLLTRLRMIHSGSPGDVIHLFNSRVPKPIHPDQPTKVFRSRVMGVETIWTIDHDDAKYFLSTGFPNFGKSPLFKAGFRRL

LGDGVFASDQRGLWAWHRSLTRPHFVRERIADVVAMEEHSHRVATWLSTQTDLGKSVDIQDIFARYTLTVGTQHLFGRCV

DSLNDLIHDRIQTGPNAADFAQNFVAAQHWAIINSLLLHPLLISLGFRIRDRATEEVRQVVDTLVQDASLSLASQIKKNN

ESDSSDQAEGGTASENLLDHLLTSGCSKELVRQECLNILLAARDTTASLLSSCIYELARDSPRKTAMWRKLKDEVERLGS

GIDVTLDQVRELKYLRAVLNESLRLHPPVWANTRHAFEDDVLPSGVFVPAGTDCRFFIREFQRNPEVWGK (0)

DAEEFDPDRWIDSRKALQVKDPFSFQPFSAGPRICLGQQFALAEASMMMIRVIEGFEGVDL

DLSDGPVGAEAPAVVLSFRGGLKVRFKK