P450s of Schizosaccharomyces pombe

              Schizosaccharomyces japonicus

              Schizosachharomyces octosporus

 

Schizosacchromyces is unusual in that it has only two CYP genes, a CYP51 and a CYP61.

 

D. Nelson

 

Aug. 10, 2009

 

>CYP51F1 S. pombe Z54096

MAFSLVSILLSIALAWYVGYIINQLTSRNSKRPPIVFHWIPFVG

SAVAYGMDPYVFFRECRAKYGDVFTFVCMGRKMTAFLGVQGNDFLFNGKLADLNAEEA

YSHLTTPVFGKDVVYDIPNHVFMEHKKFIKSGLGFSQFRSYVPLILNEMDAFLSTSPD

FGPGKEGVADLLKTMPVMTIYTASRTLQGAEVRKGFDAGFADLYHDLDQGFSPVNFVF

PWLPLPRNRRRDRAHKIMQKTYLKIIKDRRSSTENPGTDMIWTLMSCKYRDGRPLKEH

EIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKFDQYK

DMPLLNYVIQETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTATEEEY

FTHATDFDPKRWNDRVNEDENAEQIDYGYGLVTKGAASPYLPFGAGRHRCIGEQFAYM

HLSTIISKFVHDYTWTLIGKVPNVDYSSMVALPLGPVKIAWKRRN

 

>CYP51F1 Schizosachharomyces japonicus

78% to Schizosachharomyces pombe CYP51A1  SJAG_03750

MVVSMVSILLSVGLAWYVGYIANQIFNRDPKRPPVVFHWFPFIGSAVTYGIDPYVFFRQCRAKYGDVFTFVLMGRKITAY

LGVKGNDFLFNGKLADLSAEEAYSHLTTPVFGKDVVYDVPNHVLMEHKKFIKSGLGFDQFRAYVPLIVNEMHDFLAKAPT

WGPGKSGVADLLATMPVMTIYTASRTLQGEEVRAGFNAGFADLYHDLDQGFTPINFIFPWLPLPRNKRRDRAHNIMAETY

LNIIKKRRAAHENPGTDMIWTLMGSRYRDGRPLKDHEIANMMIALLMAGQHTSAATIMWILSLLGSKPEVIEMLYEEQKR

VFGKELRPLDFDSYKDLHLVNYVIREALRLHPPIHSIMRKVKRNLAVPGTKIVIPAGHHLLAAPGVTATDEEFFAHAKDF

DPERWNSRVNEDEGDVERVDYGYGLVTKGAASPFLPFGAGRHRCIGEQFATMHLCTLVSTFVREYKWTTVGGVPKVDYSS

MVALPLGPVKIAWERRE

 

>CYP51F1 Schizosachharomyces octosporus

SOCG_01956

MAISLLPLVLSGILAWYVGYILNQITNRNPKRPPIVFHWVPFFGSAIPYGIDPYAFFHECREKYGDVFTFVCMGRKMTAY

LGVKGNDFLFNGKLADLSAEEAYSHLTTPVFGKDVVYDVPNHVLMEHKKFIKSGLGFPQFRSYVPLILNEMDAFLNTSPE

FGPGKEGIADLLKTMPVMTIFTASRTLQGAEVREGFDAGFADLYHDLDQGFSPINFVFPWLPLPRNRRRDRAHNIMQQTY

LNIIKQRRSSPENPGSDMIWTLMNSKYRDGRNLKEHEIAGIMIALLMAGQHTSAATIMWALTLLGSKPEVIEMLYEEQKR

VLGEGENFHVDFDKYKDLPLLNYVIRETLRLHPPIHSIMRKVKRDLPVPDSKMVIPAGSHLLAAPGYSATHEDFFTHAKE

FCPERWGERPDDEENAEQIDYGYGLVSKGGSSPYLPFGAGRHRCIGEQFAYMHLTTIICKFVHDYTWTTVGKVPDVDYSS

MVALPLAPVKIAWKRRD

 

>CYP61A3 S. pombe Z98974 join(10345..10419,10514..12001) gene="SPAC19A8.04" 51% identical to CYP61A1. (note this name was assigned before recognition of CYP61 orthology.

This is orthologous to other fungal CYP61A1 sequences.)

MEPSDQIIRFNDKFTTISYLPWILIMQKGHIPGPRFKIPFMGSF

LDSMKPTFEKYNAKWQTGPLSCVSVFHKFVVIASERDLARKILNSPSYVQPCVVDAGK

KILKHTNWVFLDGRDHIEYRKGLNGLFTTRALASYLPAQEAVYNKYFKEFLAHSKDDY

AQYMIPFRDINVATSCRTFCGYYISDDAIKHIADEYWKITAAMELVNFPIVLPFTKVW

YGIQSRKVVMRYFMKAAAESRKNMEAGNAPACMMEEWIHEMIETRKYKSENKEGAEKP

SVLIREFSDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVREEQLRIRKG

DIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPDYTVPKDAMVIP

TLYGALHDSKVYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVCLGQRYAVNHLIA

CIGKASIMLDWKHKRTPDSDTQMIFATTFPQDMCYLKFSPFDASTVDWKNSKEAFSNE

AVSAATVETESA

 

>CYP61A1 Schizosaccharomyces japonicus

80% to S. pombe CYP61A3 (misnamed, should be CYP61A1) SJAG_03249

MTKVSFVVATFSFESLSYQIQKGTIPGPIVKIPFMGSFLDSMKPTFEKYHTKWMSGPLSCVSVFHKFVVIASEREMSRKI

LNSPNYVQPCVVDAGKKILKPNNWVFLDGKAHVEYRKGLNGLFTRRALANYLPAQEAVYNKYFKEFLDYSKDDYKQYMIP

FRDINVAVSCRTFCGSYISDDAITEIADHYWRITAAMELVNFPIVLPFTKIWYGIKARDVVMKFFMNAAAQSRKNMEAGK

PITCMMDEWIHEMIEAREYKEKGAAEGAEKPSILIRDFSDEEISLTFLSFLFASQDATSSAMTWLFQMLADNPEVLRKVR

EEQISIRNGDPKAPITIDLVDKMEYTRWVVKECLRLRPPVLMVPYRVKKGFPIRSDYTIPKDSMVVPTLWCALHDPEVFP

EPDNFIPERWGPEGTAEKSPKSWLVFGTGPHVCLGQRYAVMHLIACIGKASIELDWKHKRTEDSDTQMIFATTFPQDMCY

LKFSPFDSTKLWNELRHKKHEKPEKHEGHDLQREQSINTVASSTTESLAEGLTQTVTEA

 

>CYP61A1 Schizosachharomyces octosporus

SOCG_00808

MNQTESFLSGSQNVVRLMGYEVEYTRWTVCFALLLVCIAYDQIAYQLRKGNLPGPLIKIPFMGSFLDSMKPTFEKYKTKW

DSGPLSCVSVFHKFVVIASERDLARKILNSPSYVQPCVVDAGKKILKHTNWVFLDGRDHVEYRKGLNGLFTTRALGVYLP

AQEEVYNKYFKEFLDHSQGKYTEFMIPFRDINVATSCRTFCGHYISDDAIKHIAEEYWNITAAMELVNFPLVLPFTKVWY

GIRSRKIVMQYFMKAADASRKSMEAGNAPTCMMEEWIREMIETRKYNNSKGLDGSEKPSVLIRDFSDEEISLTFLSFLFA

SQDATSSAMTWLFQYLADYPEILQKVREEQFRIRDGDPNVPISLELLEKMTYTRMVVTECLRLRPPVLMVPYRVKKSFPI

SPEYTVPKDSMVIPTLYGALHDPKVYPDPESFKPERWEPNGLAQQNPKNWMVFGNGPHVCLGQRYAVNHLIACVGKASLM

LDWSHKRTVDSDTQMIFATTFPQDMCLLKFTPFNPATIKLHLKEDAELQTPTAAGIPSSA