Sporobolomyces roseus P450s

 

D. Nelson

 

August 9, 2009

 

6 sequences

 

>CYP51F1 Sporobolomyces roseus

55% to CYP51F1   Ustilago maydis, e_gw1.1.480.1

MGLATLALTAVLYFAGASLSIVALNVLHQLLAPRDPTKPPLVFHYVPVIGCAVSYGMDPLGFLDDCKQKYGPVFTYPLLG

RRITATLGPLGSNFVLNGKLAHVNAEEAYTHLTTPVFGTEVVYDVPNHVLMEQKKFVKFGLTTDNFRRYVGLIRQEVLGY

VSTHVFDQDVLANGVDAFTTSSEITILTASATLQGREVRQAMDKSFAELYHDLDGGFTPLNFVFPNLPLPSYRRRDRAQL

KMREFYISILEKRRASTDEPDLDMLTALQNQSYRNGEPLTDKQIAHIMIALLMAGQHTSAATGAWAILRLGENQDLQRRL

WEEQVEYHMDKETNTLNELTYENFQTPLLVATIKELLRMHPPLHSLMRKVISDCPVPSSVGSPSAEPRASHAFKKSNESK

EYVIPKGEFVLAAPGYSQVDESIWGKDAKEFRVERWLESEGGNKVPGDEDEGEEDYGWGKISRGGKSAYLPFGAGRHRCI

GEQFAQVQLGTIIATLVRELTWTLDQPFPGNDYTVRPSLFLLEAHESACTDCAPFV

 

>CYP53B2 Sporobolomyces roseus

57% to CYP53B1   Rhodotorula minuta, estExt_fgenesh1_pg.C_30339

MTQSHVFTDFFEPTTLAVYFLAAPLGAVFLYLFVPHFTSLAPLRRFPGPFWAGYTRLWLARTARVGKRSELVHREHLKHG

KFVRIGPNEVSIADPAALPIVYAHGSGSIKADFYDAFVASPVRGLFNTRNRAEHTRKRKIVSHTFAPKSVREFEPYIAST

VNLLLKKWDQLAAKAQKSPPSGTGGERMKGYAVIDSLDWFNALAFDVIGELAFGTPFGMVERDAADIVTITKEDGTVIHA

GGVQILNMRGEYSATLGCLPPWSRKYMKYIDPWFARGLESVKNLTGIARTRVNDRLEKGALDRKDILSHLQAGRDENGQP

MSKDELTMEALTQLIAGSDTTSNSSCAILFQIVSTPHAHKKLQQELDEAFSGKGMSGVLEYEDVKALPYLGACINEALRR

HSTSGIGLPRIMMDDTEVLGEVFPKGTVLSVPSYTIHWSTEFWGPDAAEFKPERWLESEEKTRQLEKQLNVFSFGPRSCV

GRNVAMIELFCFMATLVYRYDFKLVDPNQKELEVVEGFLRKPTGCQIGFKLRDATQ

 

>CYP61A1 Sporobolomyces roseus

57% to CYP61A1   Ustilago maydis, estExt_fgenesh1_pm.C_40050

MASTPHLSAQVAAASESPLFGKLAPSFDKLNIEWSTQTIATSVLAVIVTLLIAEQTLWRYRKSTLPGHSWQIPVIGQFAE

SLNPTMEAYKRSWSTPLASVSFIVIASTTEYARKILNSSTLAEPCLVRSAKQILLPENWVFLNGKVHNEYRKGLNCLFTP

QALEIYLKKQDQIYRNHFRAWLSDPNPNAQPYQLKFRDLNMETSLKVFCGDYISDKNAKEVSEKYWQITQALELVNFPFA

FPGTKVWSACRAREVTVKYLAQAAAASKKAVAAGKEPDCLLDEWVEEIHAGKVRKYEDKEMALVLLSFIFASQDAMSSSI

TFAFQLLADHPEVLAKVREEQAAVRHGDYETPMTLALLEQMPYTNAVTKEVLRYRPPVIMVPYMTKKPFPINEEYTVPKG

TMLIPSFWNSLHDEKVYPDAEEFIPERFMPGGVNDNDKDAKNWLVFGAGAHRCIGQNYVYMHMTAVIGSAAMLMNWEHAK

TPESEEIQIIATLFPKDGCHLKFTPREEAATAL

 

>CYP5139C1 Sporobolomyces roseus

estExt_fgenesh1_pg.C_10483 gene model revised

39% to CYP5139B1   Cryptococcus neoformans

MERLVDLVVYPLNHPFYTLL (2)

FAIILVVPATALWLAYSIFISPLRSSL

RDVPGPARKSLLWGNMEQIFAEQPGRSHIEWNEEFGGAARYHGLLGNQRLVLFDHAALNHVLLSNCYE

FPKPEEVRGDLAMILGKGVLFAEELIQSSAGDDHRRQRRILQPAFSPAHIRALGPVFFEHAYKLRDIWTNLVKTGADDEK

AFADSESFETYRKNKAGREEVTLDIMPWLSRLTLDIIGVAGFGYHFDSLNRSTNKLANAFAGMFSPSAVAK

KPKPSAFLLQRI

IGFFIRALPILNIAKWIPNDRIQRVRQGFATLESESRNIIESKQGEVEKDGLESVRGSKDLIALLLKSASTDAKAKM

SAEELRGQLTTFLLAGHETTSTSLTWTLWTLSRYPDMQSKLRAEVRAARKKAKDNGNDEIESDELAALPYLDAVTREILR

LESPVSATIRHAAQDDLIPLSRPIRSRSDPNSTISHIPVKKGQILFIPITAVNHSKEIFGPDASEFRPERWIGTEGDKIQ

GKVGVWSSMLTFLAGPRSCIGYKFALLELKAILATLIDDFEFSLRDPPFEIERRSAIVMRPLVIGEEPLGNRMPLRITLA

KRDEQE

 

>CYP5221A1 Sporobolomyces roseus

43% to CYP5025A1   Ustilago maydis, e_gw1.5.259.1

43% to CYP557A1   AY316198 Rhodotorula sp

N-term added back from the genomic seq

MSPLSPSISSEIFELSAFNLVVCTLLLGFVYLAWDYRDGRAIGTKRRPDLYS

PRGQQHFLLGDVVGLVKNQDRAMERF

MEAKKAIPASQKGKALSFTVPYGRRMIEISSPHMLEYVQKTNFQNYVKGPLFHRNLSGLLGNGIFVVDGPEWQSQRKATS

KIFTANNFKGVISTSIENNMSKLMTIIGRHADSGAPFDLAELFFRFTLSSFSEMAFGSNFGALSTETDEPVPFAKAFDYG

QVVMNRRFTNPFWNVTELLDGTRAKMNAANKVMDDFAYRVISEREEMGRGNFTGNQKKEAADKDLLSLYMALRDENGQPM

NRRALRDALMNLIIAGRDTTAQAASWAFFRLILHPELMTPIRKEVDELGQIDYDTYKTLVQTTATFNEALRLHPSVPKNA

WQAVGPDQIPNGPRIEAGDSVFWSDWAMGRDKSVWGLDASEFKPSRWIDEEGSVKKESQFKAHFFNGGYRHCLGMNLAMY

EGVSVIASIIRDFDLTFAPGYLETTEMVDTEFTPRYMGALTLSFAAPFMVRATRRKHA

 

>CYP5222A1 Sporobolomyces roseus

24% to CYP634A1, JGI model estExt_fgenesh1_pg.C_20096

MIGALSILLISILAYHLVARRKLTPLAPPSFSVPSLPAFLSGLVAIARLGANEDEFLKALRAEYGAVVYLPWPLRQYLIL

EAEAIECVYKAPSRVLSFFPIRVEMQSSVFDAGFETEKSLMEEFYKTHSRGLQKIQLAETMDRFVKVVEAEIGKLATRLD

ASEDGQIECNLDEWVTDIFFEASLSGLFGPLVRQSSGISKTEFRTAFDAFDGAFPLLASGLVPKPFANIVFGKGKKAQKV

LADSLEAWIEEDFPGLPTGVVRDMAQVALDNGLGKREAGKLLVSDAWALQANAPYVASQLLLYLIQAPQSILDDVVAECD

RSISLARNSSSSPQSIDHLSQNFPLLQSCLTETLRIGTSVFSIRDVEQPLHLRSRGGTEVVVPPMSRLIYATRASHLDQT

TWGDDVCKWDGRRFFDREGGGEKDEGGETVEKSKKSRQVWGFGGGISRCEGNVLATLELKSFVSTILTKFEVSLVGPTTT

DSQDYEAVEFARGEAGYRPKRRTGRVGLGVLQFDKDVMVAIRKRRLAGTE