Yarrowia lipolytica P450s

 

D. Nelson

 

August 9, 2009

 

17 sequences

 

>CYP51F1 Yarrowia lipolytica

63% to CYP51F1   S. cerevisiae

CAG82748.1

MIILTTLNNMGIPVEGWHILAGLAILAVITEIYVIGSQLLARRNKTLAPMAFYWIPWVGSSIPYGIDPYEFFEDCRNRYG

DVFSFYMLGRVMTVSLGTKGHEFVFNSKLADVSAEEAYTHLTTPVFGTGVIYDCPNSRLMEQKKFCKGALTRDAFRSYVP

KIVEEVTNFFAVNFEGKTGKADVMTTQPQMTIFTASRCLLGDEIRSKLNGDFAKLYSDLDNGFTPINFVFPNLPLPSYRK

RDLAQQKIRDTYMSVIQRRRMEKDVQDRDLIDALLKNHTYKDGKRMTPQEIAHLLIGVLMGGQHTSASTSAWMLLRLGLD

PAIQDELYQEQVDILGEADGSFRQPTYEDILTMTKLQNTIKETLRLHMPIHSIFRQVMRDLPVPGTSFVVPKGHFVMASP

GYSQQAERYFPNAKKFDPRRWMTPTEKMAESETDAEAGETVDYGFGAISKGVSSPYLPFGGGRHRCIGEQFANCQLTTLM

SCYIQNFKWTVPEGSKLPAVDTTSMITLPVHPSYIVWSKRERN

 

>CYP52F1 Yarrowia lipolytica gene for ALK1 AB010388

CAG80007.1

MSNALNLSLALGVFLLAYYGFSVIQYRIKTRKLEKKWKCGKPKD

ISRFPFSASFFIPFLVESKKNRLLEFVQWMFESQVYPGYTCKTTVFGVDMYHTVDPEN

LKAVLATQFKDFCLGERHAQFLPVLGNGIFTLDGQGWQHSRAMLRPQFARDQVSDVEM

IEEHIQYMTSRIPKDGSAFDAQELFFNLTLDTATEFLFGQSVGSQTVETNPTAVPTDM

PVHLRKSFQEDFNTAQEHLGQRARLQMFYWAWRPRELYSSGERVHAFVDHYVKKALEE

SEKHVDDGKYVFLRELAKETKDPIVLRDQALNILLAGRDTTASLLSWCLYLMARRPEV

YAKLREEVIENLGDGEDLSTITFESLKRCDYLRYVLNEVLRLYPSVPANMRYATRDTT

LPRGGGPDGMQPIVVRKGNLVSYHVFTTHRLKEFWGEDAEEFRPERWYEDGASQAKGW

EYLPFNGGPRICLGQQYALTEAGYALARIAQLYDTIENADDKPEPPVKFHALTMCHHT

GVLVKLYNSKTTKAQ

 

>CYP52F2 Yarrowia lipolytica gene for ALK2 AB010389

CAG77659.1

MIILYVLAVAVSFLIFKRVTYTMRSRELAKKWHCEEPHNLNEFP

LNLPLFFLIINASRRHELLDTLLGLFRSFAPTKTVKQVLLGSFTIIPTNDPENIKAVL

ATQFKDFCLGQRHGQLAPVLGDGIFTLDGQGWQHSRAMLRPQFARDQVSDVEMIERHV

QMMLLRIPNNKKFDIQELFFNLTLDTATEFLFGQTVGSQTVEMPNEDKSTVSDMPKDM

RKSFQEDFNVAQHHGGIRTRFQMFYWLWRPTELFSSSKRVHAFVDHYVEKALANSDEE

KSDDKYIFLRELAREVKDPRVLRDQALNILLAGRDTTAGVLSWIVYELARHPEVWKKL

RAEIHQDFGDGSDLSQITFEGLKRCEYLRFVINETLRLYPSVPLNVRYASRDTTLPRG

GGPDESKPILVRKGDTIVYNVFSMHRTEEFWGKDCDEFRPERWAEKGSRGWEYLPFNG

GPRICLGQQYALTETSYVITRICQLFTNIENADTAVEPPQKLHALTLCHLNGVFVKMT

RDEAAFAETEKLINA

 

>CYP52F3 Yarrowia lipolytica gene for ALK3 AB010390

CAG79910.1

MIIIETLIGAVVFVAVYVAFVKLDYYRRKAKFETSDMPVAYNGL

LGWKGLRHMLTVFNNDIGPVGWREVFATYGKTLKYYAFPSNTILTYDPDNIKAMLATQ

FKDFSLGLRKEALAPSLGYGIFTLDGSSWSHSRALLRPQFSREQISRLESVETHVQEM

MSCIDRNQGAYFDIQRLFFSLAMDTATDFLLGEAVGNLQEILHPEMPRTGTTFQVAFD

RAQRLGSLRIICQEAFWVVGSLFWRRDFNNTNQHIHDYVDRYVDKALLARKEKSEIYT

NPDKYIFLYELARETTNKITLRDQVLNILIAGRDTTASTLSWIFMELAKKPDIFHKLR

EAILNDFGTSCESISFESLKKCDYLRQVLNEGLRLHPVVPVNLRVAVRDTTLPRGGGP

QGDKPIFVAKGQKINYAIFWTHRDKEYWGEDAEEFRPERWETTSGGALGKGWEFLPFN

GGPRICLGQQFALTEMGYVITRLLQEYSDISIQPSDAAVKVRHSLTMCSAQGINISLT

RAKEE

 

>CYP52F4 Yarrowia lipolytica gene for ALK4 AB010391

CAG83106.1

MLTNLTIVLITLLVTYTVLTRTALRIQRARKAKQMGATLPPRVN

NGILGWYGLWLVIQNARSMKLPHTLGKRFANGPTWLTPVAGNEPINTIDPENVKAILA

TQFKDFCLGIRHRALSPSIGDGIFTLDGEGWTHSRALLRPQFSRQQISRVHSLERLMQ

ILFKLIRKENGEYFDLQNLFFMFTLDSATEFLYGASVDTLADLLGEPVEGDHGGVGEE

VRKAYQQSINNAQDISAIRTRLQGLYWIAGNIYQRNLYQKSNKGVKDFSQFFVDKALN

TSKEKLKEMEDSDNYVFLYELVKSTRNPVVIRDQLINILVAGRDTTASLLSFTFYTLG

RRPDVLKKLRAAILEDFGTSPDEITFESLKRCDYLRYVLNEVLRLYPSVPINARSATR

DTTLPRGGGPDGKQPVFVYKGQMVAYCVYWMHRDKKYWGEDALEFNPDRWDPKVQPQN

KGWEYLPFNGGPRICLGQQFALTEAGYVVTRMLQEFDTVHCKNQKEEEHPPYALDLTM

RHGEGVWVSMK

 

>CYP52F5 Yarrowia lipolytica gene for ALK5 AB010392

CAG83107.1

MLQLFGVLVLALTTALLAQLAYNKYEYNRKVKQFGCGELTVAKN

GFLGWKGIRAVLHVLKTKKGPAALKERIDAYGRTYVFHIGPAPVISTMEPENIKAMLA

TQFKDFSLGTRYRSLAPTLGDGIFTLDGHGWTHSRALLRPQFAREQVSRLDSLEAHFQ

ILKMCVDKEMREKGNDPRGFDIQNLFFLFTLDSATEFLFGSSVDSLVDFLDDPSVRTG

DHGGVDEAARKGFNNSFNHAQELCALRSRLHTLYWIVGSVVKKEPFERYNKEIKTFVD

FFAAKALKARKEKDMSLMDNDQYIFMYELVKETTNPVTLRDQMLNILLAGRDTTASML

SWIYFRLARDPKLYAKLRSAILEDFGTTPEAITFESLKQCDYLRYVLNEALRLYPVVP

INGRTATRDTTLPRGGGPDQSQPIFIPKGQTVSYSVYWTHRDPRFWGEDAEEFIPERW

DPRNGNIGRGWEYLPFNGGPRICLGQQFALTEVGYVLSRLVQTYETLETCDHKPLPPL

YNHALTMCHEEGVWVKMYKGEKA

 

>CYP52F6 Yarrowia lipolytica gene for ALK6 AB010393

CAG82620.1

MIQSVFLALAILIAYLGFAEWFSRFQHRRISKKKGCGMPPMANG

GFLGWYGLYKTYQITSERTYPHSMRMGLEAFGHTFVYPVPGTDMLQTIHPDNIKAILA

TQFKDFSLGTRHKIMLPTLGDGIFTLDGEGWTHSRALLRPQFARDQVSHVASLERHIQ

VLFKTIKKENKECDPAKGFDIQELFFMLTLDTATEFLCGDSVDSLTDYLADPTAPQLD

HSGIDENVRRAFPEAFNTAQWFCSIRAKLMKLYFFAGTVFYRKKYADANKIVHDFTDF

YVSKALAARKEKFQELDQEGKYIFLYELAKETRNPKVLRDQMLNILLAGRDTTASLLS

WVMFRMARQPETWKKLRQAVINDFGDTPDELSFESLKRCEYLRYVLNEGLRLYPSVPM

NFRVATRDTTLPKGGGPDLDQPIFIPKGGIVVYSVYHTHRAEEYWGKDTEEFIPERWD

PAEGYQIARGWEYLPFNGGPRICLGQQFALTEAGYVLARLAQEFETVTSCDDKPLPPK

YNTHLTMSHDDGVWLKME

 

>CYP52F7 Yarrowia lipolytica gene for ALK7 AB010394

CAG84028.1

MFQLFSILVLAFTTALVAQLAYNQYDYQRKVKKFGCGQLRVAEN

GLFGWKGLREVLRINKYKLGPAALKDRFEKYGKTHVFHVGPSPLITTMDPENIKAMLA

TQFKDFCLIARYKALGPMLGDGIFTLDGHGWTHSRALLRPQFAREQVSRLDSIEHHFQ

ILKKCISKEMSDKRDTQRGFDIQNLFFLMTLDTATEFLFGSSVDSLVDFLDDPSIQTG

DHGGIDEAARKGFSNAFNRAQELSSLRTRLHKLYWVIGTLAVREPYHRYNREVKTFVD

HYAAKAIKARNEKNTDLLDNDKYIFMYELVKETSNPITLRDQMLNILLAGRDTTASML

SWIYFRLARDPKRYAKLRAAVLADFGPGPENITFESLKKCDYLRYVLNESLRVYPVVP

INARTASRDTTLPRGGGPDGSQPIFVPKGQTVSYSVWWTHRDPEFWGQDAEEFIPERW

DTKNGSIGRGWEYLPFNGGPRICLGQQFALTEVGYVLSRMVQTYETLESGDTKPLPPL

YNHALTLCHQEGVWIKTE

 

>CYP52F8 Yarrowia lipolytica gene for ALK8 AB010395

CAG82058.1

MIPFTKINLELLLLPLLIIPITLFLLVLNVQVNTVHRRYKEYRL

KLARCPQQSTGLFGWWLIYAVVRCNRDRFYSAKVVDYFTKLRTFIVSLGGELVIYTSE

SENIKALLATQFSDYDLGKTRHALLFTLMGDGIFTLDGQGWAHSRALLRPQFSKETVS

PLSSLETSLQQLMAIVKRRVALKGEVDIQELFFMLTMDTATNLLYGESVDSLGDCLKE

TKKETHVMTDGKRNLLQSRDHHSNVPNEWEPIYGTYEKKGLGIGSENSLQSGVSPRDS

PVFEVSVSEDIRKAYPAALTTALEFSAMRSKLQRFYWIWGDVLYRQKFHSAVNTVHAF

SNHFVNQALKLTPQELQMKSAEKYTFLYELAQRTRDPVAIRDQLINILIAGRDTTAAL

LSFVFLCLVANPEKLAKLREGIMTDFGTSTDSITFESLKRCVYLRYVINEALRLCPPV

PINMRQANKDTTLPTGGGKNHDEPIFVAKNQIVTYSVLFMHHNQNIWGPDASEFRPER

WGEPACPKGWEYLPFNGGPRICLGQQYALTEAAYVIVRLVQEFTEIEWRDRKGLPVLF

KTHITMSLGEGLRVHMK

 

>CYP52F9 Yarrowia lipolytica

64% to CYP52F3

CAG84211.1

MLEILIGVTLFFTLWRVLVLADLKRRQKKSGYGYPPIASNGLLGWRGLVHQLSGFVKDIGPAGWGAQFKEHGKTHLYPVF

PTQLLVTRDPDNVKAILATQFKDFSFGIRKEALCPSLGYGIFTVEGSSWSHSRALLRPQFSREQISRLDSVEHHFQEFAQ

CVDNFKGEYFDIQKLFFAQSMDTSTDFLLGESVGCLKELLETRDGDESSKKFGANFQHAFDRVQRLVALRVVFQENYWII

GDVFFRNEFRQVNELIQKFVQGYVDKALKARAHKAPIYTNPDKYIFLYELARDTTNPRVLRDQVLNILIAGRDTTAATLS

WLMFELAQKPHIFHKLRKAVIEDFGTTIENISFESLKQCEYLRYVLNEVLRLHPVVPINLRVALKDTTLPRGGGPNGDEP

IFVPKGQKINYAVYWTHRDEQYWGEDAEEFKPERWDTTNHPGPLGKGWEFLPFNGGPRICLGQQFALTEMGYILTRLVQE

YSDIGIDEEYHDRPLRVRHSITMSHGDGVHVRLSREGTVVS

 

>CYP52F10 Yarrowia lipolytica

71% to CYP52F1

CAG82791.1

MLGRTLLVSDHCVDITASIFPGSSHPFTTTNPAMNTFHLLIALATFVVVYILSSSFVYQRRTNALEKQWKCGKPLYYASF

PRSLWHFYVFLRESRKHKLLEGFQRSFEAKKPFLTNKVNIFGKDIFNTCDPENLKAMLATQFKDFCLGERHAQLFPVLGD

GIFTLDGNGWQHSRAMLRPQFARDQVSDVHMVETHLKFLTSRITGSKPVDMQELFFNLTLDTATEFLFGQSVGCQIAESD

PGAYSSDMPLDLRKSFQKDFNKAQEHLGERVRLQMLYWLWNPKELQTAGARVHAFVDHYVSKALVEAEEKVDDDKYVFLR

ELARETKDPKVLRDQALNILLAGRDTTASLLSWCFYLMARDDRVWQKLRSEVIEHFGDGENLENITFESLKRCDYLRYVL

NEVLRLYPSVPANMRFATKDTTLPRGGGPVGQDPIVIRKGNVISYHVFTTHRLTQYWGEDAEEFVPERWAEGKARGWEYL

PFNGGPRICLGQQYALTEAGYVLVRLAQMYDTLENADDKPEPPVKLHALTMSHLTGVHVKLYKKN

 

>CYP52F11 Yarrowia lipolytica

69% to CYP52F2

CAG83401.1

MILLYVLAFLVSYLIFTRLRYRYQSYKLAQKWKCSPVRDVSSFPMAFPEFLFFFKSGKNNVLLDEFVNFFTKRQPNLTVG

LVLAGNDVTVTLEPENLKALLATQFKDFCLGVRHNQLAPILGDGIFTLDGQGWQHSRAMLRPQFARDQVSDVDMIERHVQ

NLVRKIPQGDSFDIQGLFFNLTLDTATEFLFGQSVSCQTVDNPAEDDSTVTDMPIPMRKSFQSDFNLAQHHGGYRVRLQM

FYWLWRPQELFTASKSVRKFVDHYVKKALVESEKEKQADDKYVFLTELAREVKSPQVLSDQALNILLAGRDTTASLLSWC

IYLLARHPETWNKLRDEILSTFGSGSDLSLITFESLKRCEYLRFVINETLRLYPSVPVNVRYATRDTTLPRGGGPDESQP

ILIRKNSVLVYSVFATHRLKKFWGEDADEFRPERWGEGISRGWEYLPFNGGPRICLGQQYALTETSYVLTRIVQLFGTLE

NADASPEPPMKLHALTMCHLTGVHVKMSTNNLP

 

>CYP52S1 Yarrowia lipolytica

42% to 52H3, 44% to 52F1

CAG81974.1

MLLPLLFAGCVFFFLHQRLNYYRRKQRAYEKWLASGKFGAGQKVADIQVQNNFLRNPFGLVQFVEFLNRTKGAFFVDFVN

QNFQKYGYILPTRTLGKVNIITCDPELIKTVLATNFKDWSIGIRRKAMFPVLGNAIFSVEGDQWTHSRAMLRPQFARDSI

ANVADLERHVTRLINVFRGYGVQSFDCQKYFFQFTLDSASDFLFGESTNSLSVQDDKSESEDTGESFNVLTQQGENAESF

AQAFKVAFTFTAIRLRLQQFYWLLRPSNKKYRDSIDTVHRLVDGYVERALENKEDTDRYIISNQLVKVCDDKKYIRDQLL

GILLAGRNTTAAVLAWIMYESARRPEIWNKMRQEVTDTFSDGPVTSDELRKCVYVRSVINEALRVYPSVPMNQRVAVRDT

FLPTGGGPDGSLPLYCPRGTKVAYSVFALHMREDFYGSDSHIFRPERWNEGVGKGWQYLPFNGGPRICLGQQFALMEASY

TLVRLVQEFDTVELDMEVVDPPPKMSSFSMVHRDGVRIRVK

 

>CYP61A1 Yarrowia lipolytica

64% to CYP61A1   Saccharomyces paradoxus

CAG84120.1

MNATQPESHIVQWLTWTQDTIGFGTLAATVLLIALLYDQFSYVKSKGSIAGPPLKMWPIIGPFLDSINPDFEKYKAQWAS

GALSCVSVFHKFVVLASDRDLTRKIFNSSAYVKPCVVDVAIKILRPTNWVFLDGKAHVDYRRGLNGLFTNRALSMYLPIQ

EGIYDTYFDKFVEMSKDGHVPYMPIFREINCALSLRTFCGNYITEEQIKEIAHDYYLVTAALELVNFPIILPFTKAWYGK

KAADKVMDIFAKCAQMAKDHIKAGGDVTCTMDAWIAIMNGRDFDSDHGTVPNKKIRKFTNKEISETIFTFLFASQDASSS

ATTWLFQIVADRPDVYAKIREEQLAVRGGDPNVPLSLELTEKMTYTNMVVKECLRLRPPVIMVPYVAKKDFPISDTYTVP

KGSMIIPTVYPSLHDPEVYERPDEFVPERWLPDGDGTKNAKNWLVFGTGPHYCLGQKYALMNFTNMIGKACMNLDFTHKV

TPLSEKVRVFATIFPDDDCLLQFKKRE

 

>CYP504A17 Yarrowia lipolytica

49% to CYP504A6   Nectria haematococca

CAG77765.1

MLLQLLSSILLAVLTFIILDWLFEIDTETPHIAGLPAKRGYPIVGAVFQLGKSHPLTYRKWSEELGPVYQVRLGNKRVVV

ANSYASVRELWITNMRANISRPESYTFHKLLSSKDGPTIGTTPWGVSARRKRRAASTALNKPSVAAYTPIINLESYKCIR

RFLRDCGGIEGEVDVSVGLATYSLNTSLTLNYGIMTADFDSTYLAEVMDIEERILRLRSTMHNTQDYIRYLRVFSRGSTV

EAAECRERRAQYMTKFLKKLKQEIDDGTDMPCIAGNILKDKESKITDADLRSICLTMVSAGFDTVPANLISCFGHLSHSY

GQTIQKRALEAIDRAYPDGDAWEMCLMDDSKVHYIVALCEETVRYGSVVPMALPRRTINDIHYQGAVIPAGTILFMNAFA

ANFDATRFHDPFKFDPERYLDLDGRLKTELGPAHFGFGAGSRMCCGIHLARKELYVALVRFILAFRILPPVDPKDMMVND

PVEIFAQPENMTTKPPPFKVRLQVRDAEKLDRWLKETKEGLGETWSVGHD

 

>CYP548P1 Yarrowia lipolytica

45% to CYP548A1   N. crassa

CAG83449.1

MSLLETIVQFVTLVAAFVGGFLALAAFYRLTLHPLAQYPGPLLCKLSSIPDVYHAWKGDRHLHYYRLHRKYGSVVRYAPN

KVSFDTGAALKHIYSGNKKEFIKSDFYEGFPAVKGFPSVHSAIIKSEHAKKKRVMSHAFSEQALASAQPYIMDKIVQLCD

LLGDNTRDTVVTPSGKPAKMISRLTSYFSFDVMGELCFGKGFDMLTSADNRFALRMTSNAAHRHLVYGCFRLLDKLPFIG

SYFFGHIVEDRKKFVEYSRAVASKRMALGDEGHRKDLFHYLMHATDPETGKGFERNELLSESNLLIVAGSDTTATSLAGC

LHYLGLHPDVRKELTRQVRAQFYSVDEIHSGSTLTKGIPLLRACIDESMRLSPPVPGVLPRTALEDVVIDGCKIPKGCDV

GVSTWSMHRNPKHFVKPLEYDPYRWLRGGQVDDSSKYSDVAQDIDEKAALNETANARAGYAPFSIGTRSCIGRALAYSEL

DCAVARICFMYDFEVCLEGKLGSGVFGYGYDDSDEFVAGRGKGGKINEWDVKKRDGEEIGTFRLWDHFAGEKEGSMMVFA

HAKKE

 

>CYP5223A1 Yarrowia lipolytica

38% to CYP5217A1   Candida albicans

CAG79538.1

MTLTDLVRAEPSGALLVSLATAYLVYLVVVAQLNPLKKCPGPWYTLYSKMPILVIFWRDSRNRYVQALHEKYGPIVRLGP

DEVSICDPKDVSKVYLGNFDKSRFYAQFGNYGAPNLFCILTKEPHIKLRKLMTGFYRKETINGTGIIQEKVDEMETVVAA

NNKQDVHTLFRYLAFDVVTRLTISDSEMLAGRNQKLVFYHEMQTSMTFWTTLQPKWWDLAAALSERQTVLGEKTSISEAS

QRCNDWTFQQTGDPDTLLDMLKSKGVSHKIAMSEVQDHIAAGHETTAVTLAFLFHRLAHDKSIQDKLYAELEPINVTLES

VDSLPYLNGLINETLRLHAAIPGAEPRVCPAGVIFQNVALPKGTIISAQPYTLHRDKAWGDPEEFRIERWSEDPPLAYFM

PFGAGIRMCIGMNVAMGELKLATAQLVKKYEIKKSSPQQQFPTMKDSYTTLPEGDNTLLFEQRAT