Bacterial phylogeny of Cavalier-Smith (2002) showing P450s

(to the level of Class)

    Note: Division rank is equivalent to phyla

 

Kingdom Bacteria (501)

 Subkingdom Negibacteria (157)

  Infrakingdom Eobacteria (7)

    Division Eobacteria (7)

       Class Chlorobacteria (1)

       Class Hadobacteria (6)

  Infrakingdom Glycobacteria (150)

    Division Cyanobacteria (28)

     Subdivision Gloeobacteria (3)

       Class Gloeobacteria (3)

     Subdivision Phycobacteria (25)

       Class Chroobacteria (9)

       Class Hormogoneae (16)

    Division Spirochaetae (0)

       Class Spirochaetes (0)

    Division Sphingobacteria (1)

       Class Flavobacteria (1)

       Class Chlorobea (green sulfur bacteria) (0)

   Superdivision Exoflagellata (121)

    Division Planctobacteria (1)

       Class Planctomycea (1)

       Class Verrucomicrobiae (0)

       Class Chlamydiae (0)

    Division Proteobacteria (120)

     Subdivision Rhodobacteria (114)

       Class Chromatibacteria (beta and gamma) (38)

       Class Alphabacteria (76)

     Subdivision Thiobacteria (6)

       Class Deltabacteria (5)

       Class Epsilobacteria (1)

     Subdivision Geobacteria (0)

       Class Ferrobacteria (0)

       Class Acidobacteria (0)

 Subkingdom Unibacteria (344)

    Division Posibacteria (338)

     Subdivision Endobacteria [low G+C] (24)

       Class Togobacteria (0)

       Class Teichobacteria [Firmicutes] (24)

       Class Mollicutes (0)

     Subdivision Actinobacteria [high G+C] (314)

       Class Arthrobacteria (1)

       Class Arabobacteria (139) (120 without M. bovis)

       Class Streptomycetes (193) 5 alleles subtracted

    Division Archaebacteria (6)

     Subdivision Euryarchaeota (4)

       Class Methanothermea (0)

       Class Archaeoglobea (0)

       Class Halomebacteria (2)

      Superclass Eurythermea

       Class Protoarchaea (0)

       Class Picrophilea (2)

     Subdivision Crenarchaeota (2)

       Class Crenarchaeota (2)

 

 

Bacterial phylogeny of Cavalier-Smith (2002) showing P450s

(to the level of Species)

    Note: Division rank is equivalent to phyla

 

Kingdom Bacteria (501)

 Subkingdom Negibacteria (157)

  Infrakingdom Eobacteria (7)

    Division Eobacteria (7)

       Class Chlorobacteria (1)

        Chloroflexus aurantiacus (1)

       Class Hadobacteria (6)

        Deinococcus radiodurans (5)

        Thermus thermophilus (1)

  Infrakingdom Glycobacteria (150)

    Division Cyanobacteria (28)

     Subdivision Gloeobacteria (3)

       Class Gloeobacteria (3)

        Gloeobacter violaceus (3)

     Subdivision Phycobacteria (25)

       Class Chroobacteria (9)

        Microcystis aeruginosa (1)

        Prochlorococcus marinus str. MIT 9313 (1)

        Synechococcus sp. WH 8102 (1)

        Synechocystis sp. (1)

        Trichodesmium erythraeum (5)

       Class Hormogoneae (16)

        Nostoc punctiforme (9)

        Nostoc sp. PCC 7120 (7) same as Anabaena

    Division Spirochaetae (0)

       Class Spirochaetes (0)

    Division Sphingobacteria (1)

       Class Flavobacteria (1)

        Microscilla sp. (1)

       Class Chlorobea (green sulfur bacteria) (0)

   Superdivision Exoflagellata (121)

    Division Planctobacteria (1)

       Class Planctomycea (1)

        Pirellula sp. (1)

       Class Verrucomicrobiae (0)

       Class Chlamydiae (0)

    Division Proteobacteria (120)

     Subdivision Rhodobacteria (114)

       Class Chromatibacteria (beta and gamma) (38)-2 alleles

        Acinetobacter calcoaceticus (1)

        Azotobacter vinelandii (1)

        Bordetella bronchiseptica (1)

        Bordetella pertussis (1)

        Bordetella parapertussis (1)

        Burkholderia fungorum (6)

        Chromobacterium violaceum ATCC 12472 (1)

        Citrobacter braakii (1)

        Erwinia herbicola (1)

        Methylococcus capsulatus (1)

        Photorhabdus luminescens (2)

        Pseudomonas aeruginosa (4)

        Pseudomonas fluorescens PfO-1 (3)

        Pseudomonas putida (2)

        Pseudomonas diterpeniphila (1)

        Pseudomonas incognita (1)

        Pseudomonas sp. (2)

        Ralstonia metallidurans (4)

        Ralstonia solanacearum (1)

        Xanthomonas axonopodis pv. citri str. 306 (1)

        Xanthomonas campestris pv. Campestris (2)

        Xylella fastidiosa (2 P450s plus alleles)

       Class Alphabacteria (76)

        Agrobacterium tumefaciens (7)

        Bradyrhizobium japonicum (16)

        Brucella melitensis (1)

        Caulobacter crescentus (4)

        Magnetospirillum magnetotacticum (4)

        Mesorhizobium loti (7)

        Novosphingobium aromaticivorans (15)

        Rhizobium etli (5)

        Rhizobium sp. (5)

        Rhodobacter sphaeroides (2)

        Rhodopseudomonas palustris (7)

        Sinorhizobium meliloti (2)

        Sphingomonas paucimobilis (1)

     Subdivision Thiobacteria (6)

       Class Deltabacteria (5)

        Myxococcus xanthus (2)

        Sorangium cellulosum = Polyangium cellulosum (2)

        Stigmatella aurantiaca (1)

       Class Epsilobacteria (1)

        Campylobacter jejuni (1)

     Subdivision Geobacteria (0)

       Class Ferrobacteria (0)

       Class Acidobacteria (0)

 Subkingdom Unibacteria (344)

    Division Posibacteria (338)

     Subdivision Endobacteria [low G+C] (24)

       Class Togobacteria (0)

       Class Teichobacteria [Firmicutes] (24)

        Bacillus anthracis str. Ames (3)

        Bacillus cereus ATCC 14579 (6)

        Bacillus subtilis (9 P450s)

        Bacillus halodurans (1)

        Bacillus megaterium (3)

        Clostridium acetobutylicum (1)

        Enterococcus faecium (1)

       Class Mollicutes (0)

     Subdivision Actinobacteria [high G+C] (314)

       Class Arthrobacteria (1)

        Arthrobacter aurescens (1)

       Class Arabobacteria (139) (120 without M. bovis)

        Actinosynnema pretiosum subsp. Auranticum (1)

        Amycolata autotrophica = Pseudonocardia autotrophica (3)

        Amycolatopsis mediterranei (8)

        Amycolatopsis orientalis (8)

        Corynebacterium efficiens YS-314 (1)

        Corynebacterium glutamicum ATCC 13032 (1)

        Lechevalieria aerocolonigenes

          = Saccharothrix aerocolonigenes (1)

        Micromonospora echinospora (2)

        Micromonospora griseorubida (2)

        Micromonospora inyoensis (1)

        Mycobacteruim abcessus (1)

        Mycobacteruim avium (4)

        Mycobacteruim bovis (19) same set as tuberculosis

        Mycobacteruim leprae (11)

        Mycobacteruim smegmatis (40)

        Mycobacteruim tuberculosis (20)

        Mycobacteruim ulcerans (1)

        Mycobacteruim species (2)

        Nocardioides sp. (1)

        Rhodococcus erythropolis (1)

        Rhodococcus fascians (1)

        Rhodococcus rhodochrous (1)

        Rhodococcus ruber (1)

        Rhodococcus sp. (2)

        Saccharopolyspora erythraea (4)

        Saccharopolyspora spinosa strain NRRL (1)

        Streptoalloteichus hindustanus (1)

       Class Streptomycetes (193) 5 alleles subtracted

        Actinomadura hibisca (1)

        Actinomadura madurae (1)

        Actinomadura verrucosospora (1)

        Actinomadura sp. ATCC 39727 (4)

        Kitasatospora griseola (2)

        Streptomyces acidiscabies (1)

        Streptomyces albofaciens strain C-0083 (1)

        Streptomyces albus (1)

        Streptomyces ansochromogenes (1)

        Streptomyces antibioticus (3)

        Streptomyces atroolivaceus (2)

        Streptomyces avermitilis (33)

        Streptomyces caelestis (1)

        Streptomyces carbophilus (1)

        Streptomyces carzinostaticus (2)

        Streptomyces chattanoogensis DSM-40241 (1)

        Streptomyces cinnamonensis (1)

        Streptomyces clavuligerus clavulanic (1)

        Streptomyces coelicolor (18)

        Streptomyces collinus (1)

        Streptomyces fradiae (3)

        Streptomyces glaucescens (1)

        Streptomyces globisporus (2)

        Streptomyces griseolus (2)

        Streptomyces griseus (5)

        Streptomyces hygroscopicus (7)

        Streptomyces kasugaensis strain A/96 (1)

        Streptomyces lavendulae (5)

        Streptomyces lividans (1)

        Streptomyces lydicus strain NRAB-0114 (3)

        Streptomyces maritimus enterocin (1)

        Streptomyces nanchangensis (2)

        Streptomyces narbonensis (1)

        Streptomyces natalensis (2)

        Streptomyces nodosus (2)

        Streptomyces noursei (2)

        Streptomyces peucetis (19)

        Streptomyces platensis (3)

        Streptomyces rimosus ssp. paromyceticus (2)

        Streptomyces rochei (3)

        Streptomyces roseochromogenes subsp. Oscitans (1)

        Streptomyces sclerotialus (1)

        Streptomyces sp. (16)

        Streptomyces spheroides (1)

        Streptomyces tendae (2)

        Streptomyces thermotolerans (1)

        Streptomyces toyocaensis (3)

        Streptomyces tubercidicus (12)

        Streptomyces venezuelae (1)

        Streptomyces virginiae (1)

        Thermobifida fusca (10)

    Division Archaebacteria (6)

     Subdivision Euryarchaeota (4)

       Class Methanothermea (0)

       Class Archaeoglobea (0)

       Class Halomebacteria (2)

        Halobacterium sp. NRC-1 (1)

        Methanosarcina barkeri (1)

Superclass Eurythermea

       Class Protoarchaea (0)

       Class Picrophilea (2)

        Ferroplasma acidarmanus (2)

     Subdivision Crenarchaeota (2)

       Class Crenarchaeota (2)

        Sulfolobus solfataricus (1)

        Sulfolobus tokodaii (1)

 

Bacterial Cytochrome P450s ranked by most abundant taxons

 

Actinobacteria (includes Mycobacterium and Streptomyces = 297 P450s)

 

Mycobacterium smegmatis (40 [mostly] unnamed P450s) Actinobacteria

CYP51    Mycobacterium smegmatis TIGR contig:3439

CYP151A1 Mycobacterium smegmatis AF102510

CYP150A2 Mycobacterium smegmatis AF107047

 

Mycobacterium bovis 19 P450s

same as tuberculosis except CYP130A1 is deleted in bovis

and CYP141A1 and CYP142A1 are pseudogenes

bovis and tuberculosis sequences are 100% matches or just 1 aa diff.

 

Mycobacterium tuberculosis (20 P450s) Actinobacteria

 

CYP138              163364..164689

CYP135A1 complement(392694..394043)

CYP135B1            659448..660866

 

operon followed by a ferredoxin order = hypo. CYP123 oxido CYP51 ferredoxin

complement(860067..860708) hypothet. protein

CYP123   complement(858862..860070)

stop of CYP123 overlaps start of this protein

complement(858035..858862) probable OXIDOREDUCTASE

CYP51    complement(856680..858035)

         complement(856471..856677) ferredoxin 208bp downstream

 

CYP126              871429..872673 adenylosuccinate lyase = 4b upstream

CYP130   complement(1403384..1404601) overlaps dowstream to regulatory protein

         complement(1402776..1403384) potential acrab operon repressor

CYP132   complement(1569582..1570967)

         complement(1568107..1569585) Probable monooxygenase,

                     similar to e.g. CYMO_ACISP P12015 cyclohexanone

                     monooxygenase (stop of CYP132 overlaps this protein start

CYP139  complement(1892268..1893560)

CYP144             2010654..2011958

CYP143A1 complement(2023445..2024626)

                    2024826..2025029 ferredoxin opposite strand 199bp

                    could share common divergent promoter

CYP140A1 complement(2130539..2131855)

CYP124A1            2540102..2541388

CYP128A1 complement(2542805..2544274) end of CYP128 overlaps Rv2267c

         complement(2541642..2542808) hypothetical protein Rv2267c

                    2546881..2547750  hypothetical protein Rv2275

                          end of Rv2275 overlaps CYP121 start

CYP121              2547747..2548937

CYP136A1            3419489..3420967

CYP141              3486506..3487708

CYP142   complement(3954321..3955517)

CYP125A1 complement(3984140..3985441)

         complement(3983121..3984140) Acyl-CoA Dehydrogenase

         complement(3981973..3983136) Acyl-CoA Dehydrogenase

         complement(3981041..3981976) hypothetical protein Rv3542c"

         complement(3980655..3981044) hypothetical protein Rv3541c

         complement(3979495..3980655) sterol carrier protein

all 6 proteins overlap

CYP137   complement(4127292..4128722)

 

Mycobacterium leprae (11 P450s; 1 gene, 10 pseudogenes, M. leprae genome is decaying)

 

NC_002677 Mycobacterium leprae strain TN complete genome

 

CYP118P 547312..547788 locus_tag="ML0447"

CYP126A2P complement(1384839..1385327) locus_tag="ML1185

CYP139A2P complement(1474970..1475188) locus_tag="ML1237

CYP139A2P complement(1474991..1475161) locus_tag="ML1238

CYP143A2P 1861010..1862160 locus_tag="ML1542

CYP125A3P 2415021..2416227 locus_tag="ML2024

CYP164A1 complement(2481544..2482848) locus_tag="ML2088

CYP164A2P cosmid B1450 GenEMBL AL035159.1

CYP118P AGAIN DUPLICATION complement(2562932..2563627) locus_tag="ML2159

CYP138A2P complement(3167438..3168451) locus_tag="ML2648

CYP130A2P GenPept AL583920.1

CYP140A3P L01095.1

 

Other Mycobacterium species

 

Mycobacterium avium

CYP51

CYP140A4 paratuberculosis

CYP243A1

L76374 paratuberculosis partial

 

Mycobacterium abscessus

CYP136B1

 

Mycobacterium ulcerans

CYP140A2

 

Mycobacterium species AF107046

CYP150A1

 

Mycobacterium sp. strain RP1 AJ310142

CYP151A2

 

Streptomyces have about one third of all known bacterial P450s, but Mycobacterium smegmatis has the most in a single bacterial species. 

 

Streptomyces species (150 P450s)

 

Streptomyces avermitilis (33 P450s) by name order Actinobacteria

CYP102B2 SAV7426

CYP102D1 SAV575

CYP105D6 SAV412_pteD

CYP105D7 SAV7469

CYP105P1 SAV413_pteC

CYP105Q1 SAV1611

CYP105R1 SAV7186

CYP107F2 SAV1171

CYP107L2 SAV1987

CYP107P2 SAV4539

CYP107U2 SAV3536

CYP107V1 SAV3519

CYP107W1 SAV2894_olmB

CYP107X1 SAV6249

CYP107Y1 SAV2377

CYP125A2 SAV5841

CYP147B1 SAV584

CYP154A2 SAV109

CYP154B2 SAV3704

CYP154C2 SAV3882

CYP154D1 SAV1308

CYP157A2 SAV3881

CYP157C2 SAV6706

CYP158A3 SAV7130 fix name in data set

CYP170A2 SAV3031 fix name in data set

CYP171A1 SAV941_aveE

CYP178A1 SAV838

CYP179A1 SAV2061

CYP180A1 SAV2165_geoC

CYP181A1 SAV2385

CYP182A1 SAV2806

CYP183A1 SAV2999

CYP184A1 SAV5111

 

Streptomyces avermitilis (33 P450s) by chromosome order

CYP154A2 SAV109

CYP105D6 SAV412_pteD

CYP105P1 SAV413_pteC

CYP102D1 SAV575

CYP147B1 SAV584

CYP178A1 SAV838

CYP171A1 SAV941_aveE

CYP107F2 SAV1171

CYP154D1 SAV1308

CYP105Q1 SAV1611

CYP107L2 SAV1987

CYP179A1 SAV2061

CYP180A1 SAV2165_geoC

CYP107Y1 SAV2377

CYP181A1 SAV2385

CYP182A1 SAV2806

CYP107W1 SAV2894_olmB

CYP183A1 SAV2999

CYP170A2 SAV3031 fix name in data set

CYP107V1 SAV3519

CYP107U2 SAV3536

CYP154B2 SAV3704

CYP157A2 SAV3881

CYP154C2 SAV3882

CYP107P2 SAV4539

CYP184A1 SAV5111

CYP125A2 SAV5841

CYP107X1 SAV6249

CYP157C2 SAV6706

CYP158A3 SAV7130 fix name in data set

CYP105R1 SAV7186

CYP102B2 SAV7426

CYP105D7 SAV7469

 

Streptomyces coelicolor (18 P450S) Actinobacteria

 

Yellow and green blocks are genes in operons or very close together

 

159A1             625127..626350

157B1  complement(627628..628164)

       complement(628247..628588) putative ATP/GTP binding protein

       complement(821848..822048) putative ferredoxin

105D5  complement(822061..823299) 12 BP APART

102B1             847649..849232 no fusion

158A2           1278746..1279960 note PKS overlaps start, hypoth. overlaps stop

157C1 complement(1740065..1741561)

      complement(1741637..1742167) putative ATP/GTP binding protein

                  3137116..3137664 putative ATP/GTP binding protein

156A1             3137661..3138893 stop and start overlap

154A1             3138905..3140131 11bp between P450s

107U1 complement(3394340..3395641) hypothet. prot overlaps start

107P1 complement(4013901..4015136)

107T1            4143070..4144254

170A1            5682098..5683483

156B1            6970033..6971376

158A1 complement(7768860..7770083)

154C1 complement(8232555..8233778) P450 ENDS OVERLAP

157A1 complement(8233775..8235016)

      complement(8235013..8235564) putative ATP/GTP binding protein

155A1 complement(8258333..8259604)

105N1            8521684..8522919

 

Streptomyces peucetis (19 P450s)

CYP105F2

CYP105P2

CYP107L9

CYP107N3

CYP107P3

CYP107U2

CYP107AH1

CYP107AJ1

CYP125A4

CYP129A2 U77891 daunorubicin "doxA"

CYP131A1 L47164 dnrQ C-term has no heme signature

CYP147F1

CYP154A3

CYP157C4

CYP164B1

CYP166B1

CYP251A1

CYP252A1

CYP253A1

 

Other Streptomyces P450s not from whole genome sequences (97 P450s plus alleles)

with S. avermitilis S. peucetis and S. coelicolor = 150 P450s

 

CYP105A1 suaC Y18556.1 Streptomyces griseolus

CYP105A3 D30815 sca-2 Streptomyces carbophilus

CYP105B1 subC SU-2 M32239 Streptomyces griseolus

CYP105B2 Cyp229, Streptomyces tubercidicus strain R-922 78% to 105B1

CYP105C1 choP M31939 Streptomyces sp.

CYP105D1 P450soy X63601 Streptomyces griseus

CYP105D2 AF071145 Streptomyces griseus

CYP105D3 AF071149 Streptomyces sclerotialus

CYP105D4 AF072709  Streptomyces lividans 69% to 105D1 67% to 105D2 82% to 105D3

CYP105D8 Cyp233, Streptomyces tubercidicus strain I-1529 68% to 105D7

CYP105D9 Streptomyces sp. JP95 GenEMBL AF509565 11774..13024

CYP105F1 AF127374  Streptomyces lavendulae LinA homolog.

CYP105H1 AF263912  Streptomyces noursei ATCC 11455 nyst gene="nysN"

CYP105H2 Streptomyces albus GenEMBL AF071143 77% to 105H1

CYP105H3 Streptomyces natalensis GenEMBL AJ278573 52789..53985

CYP105H4 Streptomyces nodosus AF357202

CYP105H5 Streptomyces griseus AJ300302 canC

CYP105K1 Y18574.1 Streptomyces tendae strain Tue901 gene="nikF"

CYP105K2 Streptomyces ansochromogenes GenEMBL AF469953  14..1246

CYP105L1 AF147703  Streptomyces fradiae demethylmacroci gene=tylHI

CYP105M1 AF200819  Streptomyces clavuligerus clavulanic

CYP105N2 Streptomyces glaucescens cytochrome P450 GenEMBL AF071144

CYP105Q2 Streptomyces sp. GenEMBL BD133549 78% to CYP105Q1

CYP105Q3 Streptomyces sp. GenEMBL BD133546 77% to 105Q1

CYP105S1 Cyp230, Streptomyces tubercidicus strain R-922 new CYP105 subfamily

CYP105S2 Streptomyces tubercidicus strain I-1529

CYP105U1 Streptomyces hygroscopicus strain NRRL 3602 AY179507

CYP105V1 Streptomyces sp. HK803

CYP107A2 Streptomyces rochei plasmid pSLA2-L NC_004808 complement(44847..46067)

CYP107B2 Streptomyces sp. GenEMBL BD133548 58% to 107B1

CYP107C1 D30759 Streptomyces thermotolerans

CYP107D1 L37200 Streptomyces antibioticus

CYP107F1 AB018074  Streptomyces griseus CDS 341..1561

CYP107G1 rapN X86780 Streptomyces hygroscopicus

CYP107L1 AF087022  Streptomyces venezuelae cytochrome P. = pikC gene AF079139

CYP107L3 CypLA, Streptomyces tubercidicus strain I-1529 60% to 107L1

CYP107L4 CypLC, Streptomyces tubercidicus strain R-922 61% to 107L1

CYP107L5 Streptomyces sp. GenEMBL BD133547 68% to 107L2

CYP107L6 Streptomyces sp. GenEMBL BD133544 72% to 107L2

CYP107L7P Streptomyces narbonensis GenEMBL AF521878  13901..14661

CYP107L8 Streptomyces sp. HK803 AY354515

CYP107N1 AF127374  Streptomyces lavendulae LinA homolog

CYP107R1 AF254925  Streptomyces maritimus enterocin

CYP107Z1 Ema11, S. rimosus ssp. paromyceticus strain R-2374 96% to CYP107Z2v1

CYP107Z2v1 Ema8, S. albofaciens strain C-0083 96% to 107Z2v2 and CYP107Z1

CYP107Z2v2 Ema3, S. rimosus ssp. paromyceticus strain BOEH-4355

CYP107Z3 Ema7, Streptomyces sp. strain IHS-0435 76% to 107Z12

CYP107Z4 Ema16, S. lydicus strain NRAB-0114 82% to 107Z12

CYP107Z5v1 Ema15, S. lydicus strain NRRL-2433 97% to 107Z5v3

CYP107Z5v2 Ema6, S. chattanoogensis DSM-40241 1 aa diff to CYP107Z5v3

CYP107Z5v3 Ema4, S. lydicus strain R-401

CYP107Z5v3 Ema10, S. kasugaensis strain A/96 1 aa diff to CYP107Z5v2

CYP107Z6 Ema5, Streptomyces sp. strain I-1525 85% to CYP107Z8

CYP107Z7 Ema17, S. tubercidicus strain DSM-40261 90% to CYP107Z8

CYP107Z8 Ema13, S. platensis strain Tu-3077 89% to CYP107Z9

CYP107Z9 Ema12, S. tubercidicus strain NRAA-7027 89% to CYP107Z8

CYP107Z10 Ema2, S. tubercidicus strain I-1529 90% to CYP107Z11

CYP107Z10 Ema14, S. platensis strain I-1548

CYP107Z11 Ema9, S. platensis strain NRAA-7479 92% to 107Z12

CYP107Z12 Ema1, Streptomyces tubercidicus strain R-922 92% to CYP107Z11

CYP107AB1 Streptomyces rochei plasmid pSLA2-L NC_004808 Links 87725..88939

CYP107AC1 Streptomyces atroolivaceus GenEMBL AF484556 60948..62147

CYP107AD1 Streptomyces hygroscopicus GenEMBL AF521896 4248..5489

CYP107AE1 Streptomyces sp. GenEMBL BD133545 50% to 107X1

CYP107AF1 Streptomyces collinus AF293355 DSM2012 rubrinomycin gene cluster

CYP107AG1 Streptomyces atroolivaceus AF484556 leinamycin biosynthetic gene cluster

CYP113B1 U08223 Streptomyces fradiae

CYP113B2 Streptomyces caelestis GenEMBL AF016585

CYP113C1 Streptomyces virginiae GenEMBL AB072568 4994..6202

CYP122A1 U65940 Streptomyces sp.

CYP122A2 rapJ X86780 Streptomyces hygroscopicus

CYP122A3 AF235504  Streptomyces hygroscopicus var. gene="fkbD"

CYP124B1 Streptomyces cinnamonensis GenEMBL AF440781 93981..95273

CYP124B2 Streptomyces nanchangensis NS3226 AF521085 complement(100196..101467)

CYP129A1 U50973 doxA Streptomyces sp.

CYP131A2 L35154 Streptomyces sp. dauQ N-terminal

CYP147C1 CypEA, Streptomyces tubercidicus strain I-1529 new subfamily CYP147C1

CYP154B1 AF145049  Streptomyces fradiae tylosin-biosynt.

CYP154J1 Streptomyces carzinostaticus subsp. Neocarzinostaticus GenEMBL AY117439

CYP154K1 Streptomyces rochei NC_004808 complement(144081..145325)

CYP157B2 Streptomyces hygroscopicus subsp. Yingchengensis AY260760

CYP157C3 Streptomyces griseus GenEMBL AB044803 3754..5328

CYP159A2 Streptomyces hygroscopicus Yingchengensis GenEMBL AY260760 983..2209

CYP160A1 AF127374 Streptomyces lavendulae LinA homolog

CYP161A1 AF263912 Streptomyces noursei ATCC 11455 nyst gene="nysL"

CYP161A2 Streptomyces natalensis GenEMBL AJ278573 81418..82611

CYP161A3 Streptomyces nodosus AF357202 amphotericin biosynthetic gene cluster CYP162A1 AJ250199 Streptomyces tendae nikkomycin gene="nikQ"

CYP163A1 AF170880 Streptomyces spheroides novobiocin gene="novI"

CYP163A2 Streptomyces roseochromogenes subsp. Oscitans AF329398 15196..16419

CYP163A3 Streptomyces antibioticus GenEMBL AF322256 22228..23478

CYP165A4 Streptomcyes toyocaensis AF039028 complement(11438..12613)

CYP165B5 Streptomyces lavendulae GenEMBL AF386507 42222..43493

CYP165C5 Streptomcyes toyocaensis strain NRRL 15009 GenEMBL U82965 AF039028

CYP165D1 Streptomcyes toyocaensis U82965 AF039028 complement(10263..11417)

CYP165E1 Streptomyces lavendulae GenEMBL AF386507 41015..42208

CYP171A2 Streptomyces nanchangensis GenEMBL AY129009

CYP185A1 CypLB, Streptomyces tubercidicus strain I-1529 new family

CYP208A1 Streptomyces globisporus GenPept AAL06697 

CYP208A2 Streptomyces carzinostaticus subsp. Neocarzinostaticus GenEMBL AY117439

CYP211A1 Streptomyces globisporus GenEMBL AY048670

CYP235A1 Streptomyces antibioticus GenEMBL AJ002638 184..1389

CYP244A1 Streptomyces sp. TP-A0274 GenEMBL AB088119 complement(5410..6594)

CYP245A1 Streptomyces sp. TP-A0274 AB088119 13260..14513

CYP246A1 Streptomyces acidiscabies GenEMBL AF393159 537..1724

 

Thermobifida fusca Streptomycetes? (10 P450s)

 

CYP154E1

CYP154F1

CYP154G1

CYP154H1

CYP157A3

CYP215A1

CYP216A1

CYP217A1

CYP218A1

CYP222A1

 

The genus Amycolatopsis Arabobacteria (16 P450s)

 

Amycolatopsis mediterranei

CYP105G1

CYP105J1

CYP107Q1

CYP107Q2

CYP165A1

CYP165B1

CYP165C1

CYP166A1

 

Amycolatopsis orientalis

CYP146

CYP165A2

CYP165A3

CYP165B2

CYP165B3

CYP165C2

CYP165C3

CYP165C4

 

The genus Actinomadura Streptomycetes? (7 P450s)

 

CYP107M1 Actinomadura hibisca

CYP165A5 Actinomadura sp. ATCC 39727 AJ561198

CYP165B2 Actinomadura sp. ATCC 39727 AJ561198

CYP165C6 Actinomadura sp. ATCC 39727 AJ561198

CYP165D2 Actinomadura sp. ATCC 39727 AJ561198

CYP208A3 Actinomadura madurae

CYP247A1 Actinomadura verrucosospora

 

The genus Micromonospora Arabobacteria (5 P450s)

CYP105L2 Micromonospora griseorubida

CYP105W1 Micromonospora echinospora AF497482

CYP105X3 Micromonospora inyoensis AF071146 1 AA DIFF TO 105X2 60% to 105A3

CYP107E1 Micromonospora griseorubida

CYP248A1 Micromonospora echinospora AF497482

 

The genus Saccharopolyspora Arabobacteria (4 P450s)

 

Saccharopolyspora erythraea

CYP107A1

CYP107B1

CYP113A1 U82823 eryK

CYP158B1 AY078067

 

Saccharopolyspora spinosa strain NRRL

CYP108C1

 

The genus Rhodococcus Arabobacteria (5 P450s)

 

Rhodococcus erythropolis

CYP116A1

 

Rhodococcus fascians

CYP105E1

 

Rhodococcus rhodochrous

CYP177A1

 

Rhodococcus ruber

CYP249A1 AF333761

 

Rhodococcus sp.

CYP102C1

CYP116B2

 

The genus Corynebacterium Arabobacteria (2 P450s)

 

CYP189A1   Corynebacterium efficiens YS-314

CYP189A2   Corynebacterium glutamicum ATCC 13032

 

Amycolata autotrophica = Pseudonocardia autotrophica Arabobacteria

CYP105A2

CYP105X1 AF525299

CYP105X2 AF071148 94% TO 105X1 60% to 105A3

 

Actinosynnema pretiosum subsp. Auranticum Arabobacteria

CYP102F1

 

Nocardioides sp. Arabobacteria

CYP145A1

 

Kitasatospora griseola Streptomycetes

CYP207A1

CYP242A1

 

Lechevalieria aerocolonigenes Arabobacteria

same as Saccharothrix aerocolonigenes

CYP245A2

 

Streptoalloteichus hindustanus Arabobacteria

CYP105xx AF071147 66% TO 105S2 (PARTIAL SEQ)

 

Arthrobacter aurescens Arthrobacteria

CYP250A1 AF146701

 

Alpha proteobacteria (48 P450s)

 

Mesorhizobium loti (7 P450s and alleles)

 

CYP112A3v1 GenPept NP_106888

CYP112A3v2 AL672112 complement(85404..86606)

CYP114A3v1 GenPept NP_106889

CYP114A3v2 AL672112 complement(84020..85309)

CYP115A2v1 NC_002678 complement(4957879..4959075)

CYP115A2v2 AL672113 41375..42607

CYP117A3v1 NC_002678 5191629..5192972

CYP117A3v2 AL672112 complement(81551..82888)

CYP127A3v1 NC_002678 4745586..4746803

CYP127A3v2 AL672114 complement(100678..101895)

CYP173A1   NC_002678 4126331..4127698

CYP202A2   GenPept NP_108561

 

Bradyrhizobium japonicum (16 P450s)

 

NC_004463 complete genome

 

CYP102A6 3173438..3176674

CYP107AA1 complement(7193424..7194725)

CYP112A1 2317922..2319127

CYP114A1 2319222..2320511

CYP115P  2317600..2317905

CYP117A1 2321653..2322996

CYP153B1 2007526..2008767

CYP153B2 7964879..7965991

CYP153B3 7966159..7967508

CYP193A1 complement(7721594..7723027)

CYP194A1 complement(3198120..3199340)

CYP195A1 complement(3142651..3143883)

CYP196A1 complement(972593..974053)

CYP199A1 1159976..1161184

CYP200A1 4251077..4252333

CYP201A1 complement(596460..597827)

 

Rhizobium etli

CYP112A4

CYP114A4

CYP115A3P

CYP117A4

CYP127A4

 

Sinorhizobium meliloti

CYP173A2

CYP202A1

 

Rhizobium sp.

CYP112A2

CYP114A2

CYP117A2

CYP127A1

CYP127A2

 

Agrobacterium tumefaciens

CYP103A1

CYP103A2

CYP103A3

CYP104A1

CYP104A2

CYP202A3

CYP206A1

 

Rhodopseudomonas palustris 7 P450s

 

CYP153B4

CYP194A2

CYP195A2

CYP196A2

CYP199A2

CYP201A2

CYP203A1

 

Rhodobacter sphaeroides (2 P450S)

 

CYP152C1 complement(38633..39955) gene = Rsph2136

CYP202B1   

 

Brucella melitensis

CYP190A1  

 

Novosphingobium aromaticivorans 15 P450s

 

CYP101B1 NZ_AAAV01000165

CYP101C1 NZ_AAAV01000133

CYP101D1 NZ_AAAV01000085

CYP101D2 NZ_AAAV01000042 complement(5601..6899) gene = Saro0208

CYP108D1 NZ_AAAV01000137

CYP111A2 NZ_AAAV01000134

CYP153C1 NZ_AAAV01000116

CYP153D1 NZ_AAAV01000178 4242..5579 gene = Saro3794

CYP196A3 NZ_AAAV01000177 gene = Saro3719

CYP203A2 NZ_AAAV01000114

CYP204A1 NZ_AAAV01000167 gene = Saro2888

CYP219A1 NZ_AAAV01000054

CYP223A1 NZ_AAAV01000162

CYP224A1 NZ_AAAV01000154 complement(32211..33443) gene = Saro2336

CYP225A1 NZ_AAAV01000151 17550..18872 gene = Saro2207

 

Caulobacter crescentus (4 P450s)

 

NC_002696 complete genome

 

CYP191A1 1051211..1052545

CYP192A1 3214816..3216192

CYP108B1 2703947..2705221

CYP153A2 61849..63153

 

Magnetospirillum magnetotacticum (4 P450s)

CYP147D1

CYP173B1

CYP201A3

CYP228A1 NZ_AAAP01002937 216..>1496 gene="Magn3794

 

Sphingomonas paucimobilis

CYP152B1

 

Beta proteobacteria (12 P450s)

 

Burkholderia fungorum 6 P450s

 

CYP105T1

CYP153B5 NZ_AAAJ02000161

CYP195A3

CYP220A1

CYP226A1 NZ_AAAJ02000018 25472..26761 gene = Bcep5906

CYP226A2 NZ_AAAJ02000018 complement(62819..64102) gene = Bcep5938

 

Ralstonia metallidurans (4 P450s)

CYP102E1

CYP108E1

CYP116B1

CYP212A2

 

Ralstonia solanacearum

CYP133B5

 

The genus Bordetella (1 P450 and allele)

CYP240A1v1 Bordetella bronchiseptica

CYP240A1v2 Bordetella pertussis

 

Chromobacterium violaceum ATCC 12472

CYP212A1

 

Gamma proteobacteria (25 P450s)

 

The genus Pseudomonas (13 P450s)

 

Pseudomonas aeruginosa (4 P450s in two strains)

 

CYP107S1 strain PAO1

CYP168A1 strain PAO1

CYP169A1 strain PAO1

CYP239A1 strain SG17M

 

Pseudomonas fluorescens PfO-1 (3 P450s)

 

CYP221A1   

CYP229A1 NZ_AAAT02000063 complement(5763..6863) gene="Pflu1316"

CYP230A1 AF318063 Pseudomonas fluorescens NCIMB 58574..59938 gene="mupO

 

Pseudomonas putida

 

CYP101

CYP238A1

 

Pseudomonas diterpeniphila

CYP226A3

 

Pseudomonas incognita

CYP111A1 P450 lin

 

Pseudomonas sp.

CYP108A1

CYP239A2

 

Xylella fastidiosa (2 P450s plus alleles)

 

CYP133B1v1 AE003889

CYP133B1v2

CYP133B1v3

CYP133B2v1

CYP133B2v2

CYP133B2v3

 

Photorhabdus luminescens subsp. laumondii TTO1 (2 P450S)

 

CYP134B1 NC_005126 complement(313663..314886) locus_tag="plu0296"

CYP234A1 complement(4894114..4895217) locus_tag="plu4183" very poor match

 

The genus Xanthomonas (3 P450s)

 

Xanthomonas axonopodis pv. citri str. 306

CYP133B3

 

Xanthomonas campestris pv. Campestris str. ATCC 33913

CYP133B4

CYP198A1

 

Citrobacter braakii (correct spelling error)

CYP176A1

 

Methylococcus capsulatus

CYP51   

 

Erwinia herbicola

CYP133A1

 

Azotobacter vinelandii

CYP152B2

 

Acinetobacter calcoaceticus

CYP153A1

 

Delta proteobacteria

 

The genus Sorangium = Polyangium (2 P450s)

 

CYP167A1 Sorangium cellulosum

CYP210A1 Polyangium cellulosum

 

Myxococcus xanthus

CYP147A1

CYP209A1

 

Stigmatella aurantiaca

CYP195A4

 

Epsilon proteobacteria

 

Campylobacter jejuni

CYP172A1

 

Firmicutes (Gram-positive bacteria) (24 P450s)

 

The genus Bacillus (22 P450s)

 

Bacillus subtilis (9 P450s)

 

CYP102A2

CYP102A3

CYP107H1 BioI

CYP107J1 cypA

CYP107K1

CYP109A1

CYP109B1 YjiB

CYP134A1 cypX

CYP152A1

 

Bacillus cereus ATCC 14579

 

CYP102A5

CYP106B2P

CYP106B3P

CYP107J3

CYP107J4P

CYP134A2P

 

Bacillus anthracis str. Ames

 

CYP102A4

CYP106B1

CYP107J2

 

Bacillus megaterium

 

CYP102A1

CYP106A1

CYP106A2

 

Bacillus halodurans

CYP197A1  

 

Clostridium acetobutylicum

CYP152A2

 

Enterococcus faecium

CYP241A1

 

Cyanobacteria (28 P450s)

 

The genus Nostoc/Anabaena

 

Nostoc punctiforme (9 P450s)

 

CYP110B2

CYP110C2 complement(34550..35941) gene = Npun2703

CYP110D2

CYP110E2

CYP110F1

CYP120B1

CYP120C1

CYP186A2

CYP197B1

CYP227A1 NZ_AAAY02000109 very poor similarity 21775..23139 gene = Npun0867

 

Nostoc sp. PCC 7120 (7 P450s) same as Anabaena  Cyanobacteria

NC_003272 complete genome

 

CYP110A1 1708114..1709493

CYP110B1 complement(4523158..4524546)

CYP110C1 5587079..5588485

CYP110D1 5678382..5679743

CYP110E1 5753083..5754450

CYP186A1 complement(1615917..1617470)

 

CYP110A2 Anabaena variabilis

 

Trichodesmium erythraeum Oscillatoriales (5 P450s)

 

CYP110E3 NZ_AABK02000017 complement(<3..1016)

CYP120A2 NZ_AABK02000021 complement(1844..2800)

CYP214A1 NZ_AABK02000024 74165..75511

CYP110D3 NZ_AABK02000068 complement(10019..11407)

CYP110G1 NZ_AABK02000081 complement(2404..3738)

 

Gloeobacter violaceus (3 P450s) Cyanobacteria

 

NC_005125, complete genome

 

CYP110E4 complement(3256348..3257724)

CYP110E5 complement(3257800..3259176)

CYP233A1 complement(2058447..2059673)

 

Synechocystis sp. Chroococcales

CYP120A1

 

Synechococcus sp. WH 8102 Chroococcales

CYP213A1

 

Microcystis aeruginosa Chroococcales

CYP149A1

 

Prochlorococcus marinus str. MIT 9313

CYP213A2

 

Deinococcus-Thermus (Genbank) Hadobacteria in the Eobacteria (Cavalier-Smith)

 

Deinococcus radiodurans (5 P450s)

 

CYP148A1

CYP155B1P

CYP155B2P NC_000959 73..384 C-term only runs off end

CYP187A1

CYP188A1

 

Thermus thermophilus

CYP175A1

 

Chloroflexi (Genbank) Chlorobacteria in the Eobacteria (Cavalier-Smith)

 

Chloroflexus aurantiacus

CYP205A1

 

Sphingobacteria

 

Microscilla sp. PRE1

CYP236A1

 

Planctomycetes

 

Pirellula sp.

CYP237A1

 

Archaea

 

The genus Sulfolobus Crenarchaeota

 

Sulfolobus solfataricus

CYP119A1

 

Sulfolobus tokodaii

CYP119A2

 

Methanosarcina barkeri Archaea; Euryarchaeota

CYP147E1

 

Halobacterium sp. NRC-1 Archaea; Euryarchaeota

CYP174A1

 

Ferroplasma acidarmanus Archaea; Euryarchaeota (2 P450S)

 

CYP231A1 NZ_AABC02000007 complement(32352..33461) gene="Faci1014

CYP232A1 NZ_AABC02000015 12150..13295