Cytochrome P450s from ferns

 

D. Nelson Sept. 12, 2006

 

CYP51G1      Ceratopteris richardii (fern)

             GenEMBL CV734775.1 CV734906.1 ESTs

HASDGVGLLILSTLLLAKVLSSLFFRSSRNKLPPKVWTFPLIGGVRKFMKGPIKMIREEY

PRLGSVFTMKVLNRNITFLLGPEESVHFFKAPESEMSQQEVYQYNVPTFGPGVVFDVDYS

VRMEQFRFFSESLRVSSLKGYVEHMVLEAKSFFSKWGDEGVVDLKSELEHLIILTASRCL

LGREVRDQLFKNVSDLFHDLDNGMQPISVLFPYLPIPAHKRRDEARKQLADIFAKIIKNR

RDSGKRETDMLQAFIDSKYRASGRYLEDHEITGLLIAALFAGQHTSSITSVWTGAYLMKN

KQFLEPVLNEQREVMSRHGDKLDYDILGEMDVLYRAIKEALRLHPPLILLLR

YSHKDFMVKTREGNEYRVPKGHVVATSPAFANRLPHIYRDPDTYDPDRFTPGREED

KAAGAFSYVSFGGGRHGCLGEPFAYMQIKTIWSHLFQNYELELLSPFPEIDWDAMVVGVK

GKVMVRYKKRSLKTEQ*

 

CYP51G1    Adiantum capillus-veneris (maidenhair fern)

           BP920200

           69% to 51G2

LAPVLDEQREVMKRHGDKLDYDVLGEMDVLYRSIKEALRLHPPLILLLRYNHKDFTVKTR

EGVEYVVPKGNVVATSPAFANRLPYIYRNP

 

CYP72      Ceratopteris richardii (fern)

           GenEMBL CV735871 EST

           45% to 72A18

ILQVYQSVYIPGVRYLPTKQNRHAWKLKRTIEEIVSEIIEKRGNGYDGTDLLEIL

MDANRQEFSQSREKLLSFREIVDECKLFFYAGHETTSTLIAWTFLLLAQNPHWQEEARKE

VLHVCGKGLPTHENLHGLKVVGMILNEALRMYPPAPYLIRETVKETKLGGLSIPRDTMFL

LPIIDFQFDRALWGDDVHDFNPQRFADGIGNACKASAAYAPFSMGPRNCLGQSFAMLESR

AIVAMTLQRFTFTVSPGYIHCPIATFTLRPQYGIQIIFKKLE*

 

CYP72clan    Adiantum capillus-veneris (maidenhair fern)

             GenEMBL BP918676 EST

             52% to 72A7, 55% to 709C9

NESLRLYPPASLILRKAENDMKLGETLIPAGTTILVPIIAWHHDERYWGADADEFRPERI

EEGIAKACKVPGAFLPFSFGPRNCIGQVFATIEAKMVLSTILQQYRFRLSPDYVHAPTMV

LTTRPQFGMPIIFEDLEQLA*

 

CYP73A59   Ceratopteris richardii (fern)

           CV735819.1

           71% TO 73A5

5    ASAGYVVRRRLQTLMYNNMFRMMFNRRFDKEDDPLFLELKRLNGERSRLAQSFEYNYGD  181

182  FIPLLRPFLRNYLKKCQKVKDDRLNLLKSFVEERQRITDVKPPSNDEKCAIDHILDAHK  358

359  NGEITYDNVLYIVENINVAAIETTLWTIEWGLAEIVNNPDIQEKMRDELNSVLGPGVPVT  538

539  EPDLPNLPYLHCVVKEAMRLHMAIPLLVPHMNLKQAKLAGYDIPAESKILVNAWWLAN  712

713  NPEWWDKPEVFNPDRFAGAEKIEANGNDFRFLPFGVGR  826

832  SCPGIILAIPIVSLVLGRLVQAFELSPPPGQKK  930

930  VDTTRHGGQFSLRIANHSVVVCKP  1001

 

CYP73      Ceratopteris richardii (fern)

           BE643259

           49% to 73A59, 54% TO 73A5, 56% TO 73A62

VFTGYGDCWRRMRRIITLPFCTNKVVQESHAVWEEEVNMAVEDLSSTRESFTSGIFIRSR

LQLMIYNIYYRMMFNRRFIDEGDPLYVELEAYNAERSRLAQSFEYNYADFLPFLRPFLKD

YLKRCEELQKKCISLFRDIFLAERRRLLSMAKVDDGDKCAADRILRDEMNHEISEDNVL

YMIESLNVQAIETTMCSLEWVLAELL

PSQIQDKVRDEIDPVLGEVMLTKPDFHKLPYLIAGGKET

LLLSMPIPLVMPDMTLEGGK

 

CYP73      Adiantum capillus-veneris (maidenhair fern)

           GenEMBL BP919958 EST

           64% to CYP73A59

EISERSVLYLIQNMNVAAIETTLWSTEWGIAELINNQHIQEKLRNELDEVLGKGVPITEP 180

DVAKLPYLQAMVKEILRMHMVIPMLVPHMNLQAVKLGKYDIPAGCRILVNAWGIANNP

 

CYP73      Adiantum capillus-veneris (maidenhair fern)

           GenEMBL BP919715 EST

           57% to 73A35P

ESPDKFWPERFMEHNIEAIGSDFRFLPFGSGRRCCPGSILALPLLALVVGRLVQTYELLP

GPGLDGVDMAELGGQSRLHLLHHSLVRIRVRA

 

CYP75?       Adiantum capillus-veneris (maidenhair fern)

             GenEMBL BP921525

             53% to 75A11, 54% to 92A11, 53% to 76C4

460 DNIKGVIANMFMGGTDTTAITIEWALAELLANPPTLKALQNELDMVVGTTRLVEEEDLTN 281

280 LPYLQAVVKETMRLHPVGPFLVPHQSSQACEVGEYKIPADTRVLVNVWAIGRDPNVWDNP 101

100 SRFDPNRFMEKGSYSHVNVYGRHFELIPFGSGK 2

 

CYP75?       Ceratopteris richardii (fern)

             GenEMBL BQ086989 EST

             46% to 75B1, 46% to 75B6, 47% to 75B3, 48% to 75B4

21  AAIHTTALTTEWGIAELLKHPHCITRLRQEMEEVLGDKKGQLIVEADIAKLTYLQCVIKE 200

201 ILRLHPVVSLLLPRMSSQECEVGGYTIPAKTLTFVNVWAIGRDEDVWENALEFRPERF 374

375 ESNKDIDVKGHHYELLPFGSGRRICAGLPVALSMVSLTLANLVHCFDLELPHGQTPDSM 551

552 NMEERKGIAANKAVPTVLVPKPRFSMNFC 638

 

CYP78A     Ceratopteris richardii (fern)

           BE643208

           53% to 78A7 N-term

MVMALWMVVELMAWGHPGGSAWGLYSFLYCSQNTKRTDAARSPPPIPGPRGLPFLGSLL

TFAGENGKCAHRRLAALASTFGARSLMAFSLGATRVVVSSDPRVARQILCNPSFNERPLT

TSARKLLFDRSIGFAPSGGHWCNLRRICAAHLFSPRRIAGHEAIRQLDCTAMLDAIAKNT

TRSGTVEVKLRPYLQHAALNNIMATVFGKRFDYSLVNGDVSGEAAELQSMVREGFELLGA

FNMXDHLPSAFXAIDS

 

CYP94?     Adiantum capillus-veneris (maidenhair fern)

           BP919714

           57% to 86C1, 60% to 94D5, in CYP86 clan

EPFTYEELKEMRYLHAALQEAMRLYPPVPHDTKLLLEEDVLPDGTRLRTGAQLAYHAYAM

GRMESIWGEDW

 

CYP98      Adiantum capillus-veneris (maidenhair fern)

           BP913791

           55% to 98A3 mid region

LSITFTRIAFGKRFVDGDGNIDPQGVEFKDIIAQGMKLGACIKTPEFILWIRWMFPLQKE

EFGKHGMRRDNLTKLIMQEHTIARETSGETKQHFIDALLSLQKQHDLTDTHIIG

 

CYP704B9   Ceratopteris richardii (fern)

           GenEMBL BE643294 EST

           50% to 704B2

           N-term to I-helix, % id will increase with C-term

HVEHILKANFRNYPKEIVYMS

FVDVLGDGIFNVDGEKWLHQRKVAILEFSSAKLRDFSTRAYRTHALRLLNV

LLDAWKSGLVVDMQDLFMRLTLDSICNIGFGVNLGSLSSELPDVPFMRSFDDSNKLIIRR

YVDYAWKIKRALNVGGEAKLKKCVEIVDGFIYNVINIRRMEMTASRARGEELQKEDLLSR

FMSLSNEDGSPYSDKQLRDIVVNFIIAGRDTTALTLSWLFFMLCKNPTGIKNFCREL

 

CYP704      Ceratopteris richardii (fern)

            CV735325.1

            48% to 704B1

MAGGLVLQGIILLAAAVTAWIFVHRLRQRNSKDPKTWPLLGSQLE

TALNFYRLHDWIFSFFRNDHRTVKLCIPSGNLFFTVDPVNVEHILKANFRNYPKGEKSNY

IARDFLGDGIFNSDGDVWKRHRKVASFEFSNRKLRDFSADAFREDALRLVRIVHSAALSD

KPIDLQDLFMRMTLDSICQVGFGVSLGSLSPELPDIPFAKAFDTVNEIITSRFFNAFWRI

EKALNIGKEKILKKEVGVLNSFTANIIDQRSLELQGNNRSKVDLLSRFMSYNDDEPDTFT

DKELRDAILNFVIAGRDTSAITLSWFIFCICAHPHVADMVFEE

 

CYP709?    Adiantum capillus-veneris (maidenhair fern)

           BP917897

           39% to 709B1 N-term, 51% to pine EST CF672445.1

333 RLLPHYYHWKKTYGDLFVFWIGYRAVLPIRNIELVKEILSNTFAHFQKLSMRPEARTLVG 154

153 ESVASLEGEQWAQHRRIVGSAFVAEKPKVCFSMFVSCSLSI 31

 

CYP709     Adiantum capillus-veneris (maidenhair fern)

           BP914549

           50% to 709B2 I-helix to PERF

QDRLHGDGYGNDLLGLMLAANKGELVGNQKNLIMGLNEVIDECKTFFFAGHETTASLLTF

MFLLLATHPEWQERLREEVFELCGRTEPPTADSLNHLKLVGMVINESLRLYPTASAIFRE

AENDVKLGETLIPAGTTILVPIVAWHHDERYWGADANEFRPERFEEGIAKA

 

CYP        Ceratopteris richardii (fern)

           BQ087157

           34% to 709B2, 38% to 734A8 tomato N-term

MRLVLPELLYTEVWVALLVVVLGGPLVYATQTLLIKWLRVRRARSMFEKQGFKCFQ

RCFFTGNMPELVAINRKVRSEPMPDIVHAIAPRLLPHLYKWSETYGEHFIYWVGFQPRYI

IRDPEQGKELLSTKSGHFKKVS