Cytochrome P450s from ferns

D. Nelson Sept. 12, 2006

CYP51G1 Ceratopteris richardii (fern)

GenEMBL CV734775.1 CV734906.1 ESTs

HASDGVGLLILSTLLLAKVLSSLFFRSSRNKLPPKVWTFPLIGGVRKFMKGPIKMIREEY

PRLGSVFTMKVLNRNITFLLGPEESVHFFKAPESEMSQQEVYQYNVPTFGPGVVFDVDYS

VRMEQFRFFSESLRVSSLKGYVEHMVLEAKSFFSKWGDEGVVDLKSELEHLIILTASRCL

LGREVRDQLFKNVSDLFHDLDNGMQPISVLFPYLPIPAHKRRDEARKQLADIFAKIIKNR

RDSGKRETDMLQAFIDSKYRASGRYLEDHEITGLLIAALFAGQHTSSITSVWTGAYLMKN

KQFLEPVLNEQREVMSRHGDKLDYDILGEMDVLYRAIKEALRLHPPLILLLR

YSHKDFMVKTREGNEYRVPKGHVVATSPAFANRLPHIYRDPDTYDPDRFTPGREED

KAAGAFSYVSFGGGRHGCLGEPFAYMQIKTIWSHLFQNYELELLSPFPEIDWDAMVVGVK

GKVMVRYKKRSLKTEQ*

CYP51G1 Adiantum capillus-veneris (maidenhair fern)

BP920200

69% to 51G2

LAPVLDEQREVMKRHGDKLDYDVLGEMDVLYRSIKEALRLHPPLILLLRYNHKDFTVKTR

EGVEYVVPKGNVVATSPAFANRLPYIYRNP

CYP72 Ceratopteris richardii (fern)

GenEMBL CV735871 EST

45% to 72A18

ILQVYQSVYIPGVRYLPTKQNRHAWKLKRTIEEIVSEIIEKRGNGYDGTDLLEIL

MDANRQEFSQSREKLLSFREIVDECKLFFYAGHETTSTLIAWTFLLLAQNPHWQEEARKE

VLHVCGKGLPTHENLHGLKVVGMILNEALRMYPPAPYLIRETVKETKLGGLSIPRDTMFL

LPIIDFQFDRALWGDDVHDFNPQRFADGIGNACKASAAYAPFSMGPRNCLGQSFAMLESR

AIVAMTLQRFTFTVSPGYIHCPIATFTLRPQYGIQIIFKKLE*

CYP72clan Adiantum capillus-veneris (maidenhair fern)

GenEMBL BP918676 EST

52% to 72A7, 55% to 709C9

NESLRLYPPASLILRKAENDMKLGETLIPAGTTILVPIIAWHHDERYWGADADEFRPERI

EEGIAKACKVPGAFLPFSFGPRNCIGQVFATIEAKMVLSTILQQYRFRLSPDYVHAPTMV

LTTRPQFGMPIIFEDLEQLA*

CYP73A59 Ceratopteris richardii (fern)

CV735819.1

71% TO 73A5

5 ASAGYVVRRRLQTLMYNNMFRMMFNRRFDKEDDPLFLELKRLNGERSRLAQSFEYNYGD 181

182 FIPLLRPFLRNYLKKCQKVKDDRLNLLKSFVEERQRITDVKPPSNDEKCAIDHILDAHK 358

359 NGEITYDNVLYIVENINVAAIETTLWTIEWGLAEIVNNPDIQEKMRDELNSVLGPGVPVT 538

539 EPDLPNLPYLHCVVKEAMRLHMAIPLLVPHMNLKQAKLAGYDIPAESKILVNAWWLAN 712

713 NPEWWDKPEVFNPDRFAGAEKIEANGNDFRFLPFGVGR 826

832 SCPGIILAIPIVSLVLGRLVQAFELSPPPGQKK 930

930 VDTTRHGGQFSLRIANHSVVVCKP 1001

CYP73 Ceratopteris richardii (fern)

BE643259

49% to 73A59, 54% TO 73A5, 56% TO 73A62

VFTGYGDCWRRMRRIITLPFCTNKVVQESHAVWEEEVNMAVEDLSSTRESFTSGIFIRSR

LQLMIYNIYYRMMFNRRFIDEGDPLYVELEAYNAERSRLAQSFEYNYADFLPFLRPFLKD

YLKRCEELQKKCISLFRDIFLAERRRLLSMAKVDDGDKCAADRILRDEMNHEISEDNVL

YMIESLNVQAIETTMCSLEWVLAELL

PSQIQDKVRDEIDPVLGEVMLTKPDFHKLPYLIAGGKET

LLLSMPIPLVMPDMTLEGGK

CYP73 Adiantum capillus-veneris (maidenhair fern)

GenEMBL BP919958 EST

64% to CYP73A59

EISERSVLYLIQNMNVAAIETTLWSTEWGIAELINNQHIQEKLRNELDEVLGKGVPITEP 180

DVAKLPYLQAMVKEILRMHMVIPMLVPHMNLQAVKLGKYDIPAGCRILVNAWGIANNP

CYP73 Adiantum capillus-veneris (maidenhair fern)

GenEMBL BP919715 EST

57% to 73A35P

ESPDKFWPERFMEHNIEAIGSDFRFLPFGSGRRCCPGSILALPLLALVVGRLVQTYELLP

GPGLDGVDMAELGGQSRLHLLHHSLVRIRVRA

CYP75? Adiantum capillus-veneris (maidenhair fern)

GenEMBL BP921525

53% to 75A11, 54% to 92A11, 53% to 76C4

460 DNIKGVIANMFMGGTDTTAITIEWALAELLANPPTLKALQNELDMVVGTTRLVEEEDLTN 281

280 LPYLQAVVKETMRLHPVGPFLVPHQSSQACEVGEYKIPADTRVLVNVWAIGRDPNVWDNP 101

100 SRFDPNRFMEKGSYSHVNVYGRHFELIPFGSGK 2

CYP75? Ceratopteris richardii (fern)

GenEMBL BQ086989 EST

46% to 75B1, 46% to 75B6, 47% to 75B3, 48% to 75B4

21 AAIHTTALTTEWGIAELLKHPHCITRLRQEMEEVLGDKKGQLIVEADIAKLTYLQCVIKE 200

201 ILRLHPVVSLLLPRMSSQECEVGGYTIPAKTLTFVNVWAIGRDEDVWENALEFRPERF 374

375 ESNKDIDVKGHHYELLPFGSGRRICAGLPVALSMVSLTLANLVHCFDLELPHGQTPDSM 551

552 NMEERKGIAANKAVPTVLVPKPRFSMNFC 638

CYP78A Ceratopteris richardii (fern)

BE643208

53% to 78A7 N-term

MVMALWMVVELMAWGHPGGSAWGLYSFLYCSQNTKRTDAARSPPPIPGPRGLPFLGSLL

TFAGENGKCAHRRLAALASTFGARSLMAFSLGATRVVVSSDPRVARQILCNPSFNERPLT

TSARKLLFDRSIGFAPSGGHWCNLRRICAAHLFSPRRIAGHEAIRQLDCTAMLDAIAKNT

TRSGTVEVKLRPYLQHAALNNIMATVFGKRFDYSLVNGDVSGEAAELQSMVREGFELLGA

FNMXDHLPSAFXAIDS

CYP94? Adiantum capillus-veneris (maidenhair fern)

BP919714

57% to 86C1, 60% to 94D5, in CYP86 clan

EPFTYEELKEMRYLHAALQEAMRLYPPVPHDTKLLLEEDVLPDGTRLRTGAQLAYHAYAM

GRMESIWGEDW

CYP98 Adiantum capillus-veneris (maidenhair fern)

BP913791

55% to 98A3 mid region

LSITFTRIAFGKRFVDGDGNIDPQGVEFKDIIAQGMKLGACIKTPEFILWIRWMFPLQKE

EFGKHGMRRDNLTKLIMQEHTIARETSGETKQHFIDALLSLQKQHDLTDTHIIG

CYP704B9 Ceratopteris richardii (fern)

GenEMBL BE643294 EST

50% to 704B2

N-term to I-helix, % id will increase with C-term

HVEHILKANFRNYPKEIVYMS

FVDVLGDGIFNVDGEKWLHQRKVAILEFSSAKLRDFSTRAYRTHALRLLNV

LLDAWKSGLVVDMQDLFMRLTLDSICNIGFGVNLGSLSSELPDVPFMRSFDDSNKLIIRR

YVDYAWKIKRALNVGGEAKLKKCVEIVDGFIYNVINIRRMEMTASRARGEELQKEDLLSR

FMSLSNEDGSPYSDKQLRDIVVNFIIAGRDTTALTLSWLFFMLCKNPTGIKNFCREL

CYP704 Ceratopteris richardii (fern)

CV735325.1

48% to 704B1

MAGGLVLQGIILLAAAVTAWIFVHRLRQRNSKDPKTWPLLGSQLE

TALNFYRLHDWIFSFFRNDHRTVKLCIPSGNLFFTVDPVNVEHILKANFRNYPKGEKSNY

IARDFLGDGIFNSDGDVWKRHRKVASFEFSNRKLRDFSADAFREDALRLVRIVHSAALSD

KPIDLQDLFMRMTLDSICQVGFGVSLGSLSPELPDIPFAKAFDTVNEIITSRFFNAFWRI

EKALNIGKEKILKKEVGVLNSFTANIIDQRSLELQGNNRSKVDLLSRFMSYNDDEPDTFT

DKELRDAILNFVIAGRDTSAITLSWFIFCICAHPHVADMVFEE

CYP709? Adiantum capillus-veneris (maidenhair fern)

BP917897

39% to 709B1 N-term, 51% to pine EST CF672445.1

333 RLLPHYYHWKKTYGDLFVFWIGYRAVLPIRNIELVKEILSNTFAHFQKLSMRPEARTLVG 154

153 ESVASLEGEQWAQHRRIVGSAFVAEKPKVCFSMFVSCSLSI 31

CYP709 Adiantum capillus-veneris (maidenhair fern)

BP914549

50% to 709B2 I-helix to PERF

QDRLHGDGYGNDLLGLMLAANKGELVGNQKNLIMGLNEVIDECKTFFFAGHETTASLLTF

MFLLLATHPEWQERLREEVFELCGRTEPPTADSLNHLKLVGMVINESLRLYPTASAIFRE

AENDVKLGETLIPAGTTILVPIVAWHHDERYWGADANEFRPERFEEGIAKA

CYP Ceratopteris richardii (fern)

BQ087157

34% to 709B2, 38% to 734A8 tomato N-term

MRLVLPELLYTEVWVALLVVVLGGPLVYATQTLLIKWLRVRRARSMFEKQGFKCFQ

RCFFTGNMPELVAINRKVRSEPMPDIVHAIAPRLLPHLYKWSETYGEHFIYWVGFQPRYI

IRDPEQGKELLSTKSGHFKKVS