Cytochrome P450s from ferns
D. Nelson Sept. 12, 2006
CYP51G1 Ceratopteris richardii (fern)
GenEMBL CV734775.1 CV734906.1 ESTs
HASDGVGLLILSTLLLAKVLSSLFFRSSRNKLPPKVWTFPLIGGVRKFMKGPIKMIREEY
PRLGSVFTMKVLNRNITFLLGPEESVHFFKAPESEMSQQEVYQYNVPTFGPGVVFDVDYS
VRMEQFRFFSESLRVSSLKGYVEHMVLEAKSFFSKWGDEGVVDLKSELEHLIILTASRCL
LGREVRDQLFKNVSDLFHDLDNGMQPISVLFPYLPIPAHKRRDEARKQLADIFAKIIKNR
RDSGKRETDMLQAFIDSKYRASGRYLEDHEITGLLIAALFAGQHTSSITSVWTGAYLMKN
KQFLEPVLNEQREVMSRHGDKLDYDILGEMDVLYRAIKEALRLHPPLILLLR
YSHKDFMVKTREGNEYRVPKGHVVATSPAFANRLPHIYRDPDTYDPDRFTPGREED
KAAGAFSYVSFGGGRHGCLGEPFAYMQIKTIWSHLFQNYELELLSPFPEIDWDAMVVGVK
GKVMVRYKKRSLKTEQ*
CYP51G1 Adiantum capillus-veneris (maidenhair fern)
BP920200
69% to
51G2
LAPVLDEQREVMKRHGDKLDYDVLGEMDVLYRSIKEALRLHPPLILLLRYNHKDFTVKTR
EGVEYVVPKGNVVATSPAFANRLPYIYRNP
CYP72 Ceratopteris
richardii (fern)
GenEMBL CV735871 EST
45% to
72A18
ILQVYQSVYIPGVRYLPTKQNRHAWKLKRTIEEIVSEIIEKRGNGYDGTDLLEIL
MDANRQEFSQSREKLLSFREIVDECKLFFYAGHETTSTLIAWTFLLLAQNPHWQEEARKE
VLHVCGKGLPTHENLHGLKVVGMILNEALRMYPPAPYLIRETVKETKLGGLSIPRDTMFL
LPIIDFQFDRALWGDDVHDFNPQRFADGIGNACKASAAYAPFSMGPRNCLGQSFAMLESR
AIVAMTLQRFTFTVSPGYIHCPIATFTLRPQYGIQIIFKKLE*
CYP72clan Adiantum capillus-veneris (maidenhair fern)
GenEMBL
BP918676 EST
52% to 72A7, 55% to 709C9
NESLRLYPPASLILRKAENDMKLGETLIPAGTTILVPIIAWHHDERYWGADADEFRPERI
EEGIAKACKVPGAFLPFSFGPRNCIGQVFATIEAKMVLSTILQQYRFRLSPDYVHAPTMV
LTTRPQFGMPIIFEDLEQLA*
CYP73A59 Ceratopteris richardii (fern)
CV735819.1
71% TO
73A5
5
ASAGYVVRRRLQTLMYNNMFRMMFNRRFDKEDDPLFLELKRLNGERSRLAQSFEYNYGD 181
182 FIPLLRPFLRNYLKKCQKVKDDRLNLLKSFVEERQRITDVKPPSNDEKCAIDHILDAHK 358
359
NGEITYDNVLYIVENINVAAIETTLWTIEWGLAEIVNNPDIQEKMRDELNSVLGPGVPVT 538
539
EPDLPNLPYLHCVVKEAMRLHMAIPLLVPHMNLKQAKLAGYDIPAESKILVNAWWLAN 712
713
NPEWWDKPEVFNPDRFAGAEKIEANGNDFRFLPFGVGR 826
832 SCPGIILAIPIVSLVLGRLVQAFELSPPPGQKK 930
930 VDTTRHGGQFSLRIANHSVVVCKP 1001
CYP73 Ceratopteris
richardii (fern)
BE643259
49% to
73A59, 54% TO 73A5, 56% TO 73A62
VFTGYGDCWRRMRRIITLPFCTNKVVQESHAVWEEEVNMAVEDLSSTRESFTSGIFIRSR
LQLMIYNIYYRMMFNRRFIDEGDPLYVELEAYNAERSRLAQSFEYNYADFLPFLRPFLKD
YLKRCEELQKKCISLFRDIFLAERRRLLSMAKVDDGDKCAADRILRDEMNHEISEDNVL
YMIESLNVQAIETTMCSLEWVLAELL
PSQIQDKVRDEIDPVLGEVMLTKPDFHKLPYLIAGGKET
LLLSMPIPLVMPDMTLEGGK
CYP73 Adiantum
capillus-veneris (maidenhair fern)
GenEMBL BP919958 EST
64% to
CYP73A59
EISERSVLYLIQNMNVAAIETTLWSTEWGIAELINNQHIQEKLRNELDEVLGKGVPITEP
180
DVAKLPYLQAMVKEILRMHMVIPMLVPHMNLQAVKLGKYDIPAGCRILVNAWGIANNP
CYP73 Adiantum
capillus-veneris (maidenhair fern)
GenEMBL BP919715 EST
57% to
73A35P
ESPDKFWPERFMEHNIEAIGSDFRFLPFGSGRRCCPGSILALPLLALVVGRLVQTYELLP
GPGLDGVDMAELGGQSRLHLLHHSLVRIRVRA
CYP75? Adiantum
capillus-veneris (maidenhair fern)
GenEMBL BP921525
53% to 75A11, 54% to 92A11, 53% to 76C4
460
DNIKGVIANMFMGGTDTTAITIEWALAELLANPPTLKALQNELDMVVGTTRLVEEEDLTN 281
280
LPYLQAVVKETMRLHPVGPFLVPHQSSQACEVGEYKIPADTRVLVNVWAIGRDPNVWDNP 101
100 SRFDPNRFMEKGSYSHVNVYGRHFELIPFGSGK
2
CYP75?
Ceratopteris richardii (fern)
GenEMBL BQ086989 EST
46% to 75B1, 46% to 75B6, 47% to 75B3, 48% to 75B4
21
AAIHTTALTTEWGIAELLKHPHCITRLRQEMEEVLGDKKGQLIVEADIAKLTYLQCVIKE 200
201
ILRLHPVVSLLLPRMSSQECEVGGYTIPAKTLTFVNVWAIGRDEDVWENALEFRPERF 374
375
ESNKDIDVKGHHYELLPFGSGRRICAGLPVALSMVSLTLANLVHCFDLELPHGQTPDSM 551
552
NMEERKGIAANKAVPTVLVPKPRFSMNFC 638
CYP78A Ceratopteris richardii
(fern)
BE643208
53% to
78A7 N-term
MVMALWMVVELMAWGHPGGSAWGLYSFLYCSQNTKRTDAARSPPPIPGPRGLPFLGSLL
TFAGENGKCAHRRLAALASTFGARSLMAFSLGATRVVVSSDPRVARQILCNPSFNERPLT
TSARKLLFDRSIGFAPSGGHWCNLRRICAAHLFSPRRIAGHEAIRQLDCTAMLDAIAKNT
TRSGTVEVKLRPYLQHAALNNIMATVFGKRFDYSLVNGDVSGEAAELQSMVREGFELLGA
FNMXDHLPSAFXAIDS
CYP94? Adiantum
capillus-veneris (maidenhair fern)
BP919714
57% to
86C1, 60% to 94D5, in
CYP86 clan
EPFTYEELKEMRYLHAALQEAMRLYPPVPHDTKLLLEEDVLPDGTRLRTGAQLAYHAYAM
GRMESIWGEDW
CYP98
Adiantum
capillus-veneris (maidenhair fern)
BP913791
55% to
98A3 mid region
LSITFTRIAFGKRFVDGDGNIDPQGVEFKDIIAQGMKLGACIKTPEFILWIRWMFPLQKE
EFGKHGMRRDNLTKLIMQEHTIARETSGETKQHFIDALLSLQKQHDLTDTHIIG
CYP704B9 Ceratopteris richardii (fern)
GenEMBL BE643294 EST
50% to
704B2
N-term
to I-helix, % id will increase with C-term
HVEHILKANFRNYPKEIVYMS
FVDVLGDGIFNVDGEKWLHQRKVAILEFSSAKLRDFSTRAYRTHALRLLNV
LLDAWKSGLVVDMQDLFMRLTLDSICNIGFGVNLGSLSSELPDVPFMRSFDDSNKLIIRR
YVDYAWKIKRALNVGGEAKLKKCVEIVDGFIYNVINIRRMEMTASRARGEELQKEDLLSR
FMSLSNEDGSPYSDKQLRDIVVNFIIAGRDTTALTLSWLFFMLCKNPTGIKNFCREL
CYP704 Ceratopteris
richardii (fern)
CV735325.1
48% to 704B1
MAGGLVLQGIILLAAAVTAWIFVHRLRQRNSKDPKTWPLLGSQLE
TALNFYRLHDWIFSFFRNDHRTVKLCIPSGNLFFTVDPVNVEHILKANFRNYPKGEKSNY
IARDFLGDGIFNSDGDVWKRHRKVASFEFSNRKLRDFSADAFREDALRLVRIVHSAALSD
KPIDLQDLFMRMTLDSICQVGFGVSLGSLSPELPDIPFAKAFDTVNEIITSRFFNAFWRI
EKALNIGKEKILKKEVGVLNSFTANIIDQRSLELQGNNRSKVDLLSRFMSYNDDEPDTFT
DKELRDAILNFVIAGRDTSAITLSWFIFCICAHPHVADMVFEE
CYP709? Adiantum capillus-veneris (maidenhair fern)
BP917897
39% to
709B1 N-term, 51% to pine EST CF672445.1
333
RLLPHYYHWKKTYGDLFVFWIGYRAVLPIRNIELVKEILSNTFAHFQKLSMRPEARTLVG 154
153
ESVASLEGEQWAQHRRIVGSAFVAEKPKVCFSMFVSCSLSI 31
CYP709 Adiantum
capillus-veneris (maidenhair fern)
BP914549
50% to
709B2 I-helix to PERF
QDRLHGDGYGNDLLGLMLAANKGELVGNQKNLIMGLNEVIDECKTFFFAGHETTASLLTF
MFLLLATHPEWQERLREEVFELCGRTEPPTADSLNHLKLVGMVINESLRLYPTASAIFRE
AENDVKLGETLIPAGTTILVPIVAWHHDERYWGADANEFRPERFEEGIAKA
CYP Ceratopteris richardii (fern)
BQ087157
34% to
709B2, 38% to 734A8 tomato N-term
MRLVLPELLYTEVWVALLVVVLGGPLVYATQTLLIKWLRVRRARSMFEKQGFKCFQ
RCFFTGNMPELVAINRKVRSEPMPDIVHAIAPRLLPHLYKWSETYGEHFIYWVGFQPRYI
IRDPEQGKELLSTKSGHFKKVS