Lolium rigidum P450s

last modified Oct 12, 2001 D. Nelson The Lolium rigidum (annual ryegrass) P450s deposited in Genbank April 18, 2001 have been placed in this file. They are not named yet. There are 16 of these sequences. They are in the CYP71 clan (CYP71, CYP81 and CYP89 familes) and the CYP72 clan (CYP72, and CYP714 familes). Fischer,T.C., Klattig,J.T. and Gierl,A. A general cloning strategy of divergent plant cytochrome P450 genes and its application in Lolium rigidum and Ocimum basilicum Unpublished >Lolium rigidum clone Lol-83 AF321870 78% to 72A18 (rice) 54% to 72A15 (Arabidopsis) MATRALQMLGEASPWNLACAAAAMAVLWLAAWILEWAWWTPRRL GRALEAQGLKGTRYRLFTGDVPENARLNKEARSKPLPLGSHDIIPRVQPMISNAIKEN GKLSFTWFGPEPRVTILDPESVREILSNKFGHYGKPRSSRFGKLLANGLVNHQGEKWA KHRRILNPAFHHEKIKRMLPVFSACSEEMITRWENSMSSQGVSEVDVWPEFQNLTGDV ISRTAFGSSYQEGTKIFQLQGEQAERLMQAFQTLFIPGYWFLPTKNNRRMRAIDREIC TILRGIIEKKDRAIKSGEASSDDLLGLLLESNRRESNGKADLGMSTEDIIEECKLFYF AGMETTSVLLTWTLIVLSMHPEWQEQARKEVLHHFGRTKPDFENLSRLKIVTMVLYEV LRLYPPAIFVTRRTYKAMELGGITYPAGVNLMLPILFIHHDPNIWGKDASEFNPQRFA DGISNAVKHPAAFFPFGGGPRICIGQNFALLEAKMALSTILQRFSFELSPSYTHAPYT VLTLHPQHGAPIVLRKI* >Lolium rigidum clone Lol-79 AF321869 79% to 72A18 (rice) MATRALQMLGEASPWNLACAAAAMAVLWLAAWILEWAWWTPRRL GRALEAQGLKGTRYRLFTGDVPENARLNKEARSKPLPLGSHDIIPRVQPMISNAIKEN GKLSFTWFGPEPRVTILDPESVREILSNKFGHYGKPRSSRFGKLLANGLVNHQGEKWA KHRRILNPAFHHEKIKRMLPVFSACSEEMITRWENSMSSQGVSEVDVWPEFQNLTGDV ISRTAFGSSYQEGTKIFQLQGEQAERLMQAFQTLFIPGYWFLPTKNNRRMREIDREIC TILRGIIEKKDRAIKSGEASSDDLLGLLLESNRRESNGKADLGMSTEDIIEECKLFYF AGMETTSVLLTWTLIVLSMHPEWQEQARKEVLHHFGRTTPDFENLSRLKIVTMVLYEV LRLYPPAIFVTRRTYKAMELGGITYPAGVNLMLPILFIHHDPNIWGKDASEFNPQRFA DGISNAVKHPAAFFPFGGGPRICIGQNFALLEAKMALSTILQRFSFELSPSYTHAPYT VLTLHPQHGAPIVLRKI* >Lolium rigidum clone Lol-78 AF321868 78% to 72A18 (rice) MATRALQMLREASPWSLAGAAAAMALLWLAAWIVEWAWWTPRRL RRALQAQGLRGTQYRLFTGDVPENARLNREARSKPLPLGSHDIIQRVQPMFSNVIKEN GKFSFTWFGPTPRVMIPDPELVREVLSNKFGHYGKQKSSRLGKLLANGLANHQGEKWA KHRRILNPAFHNEKIKRMLPVFATCCEEMITRWDNSMSTEGSSEIDIWPEFQNLTGDV ISRTAFGSNYQEGMKIFQLQGELAERLIMAFQTIFIPGYWFLPTKNNKRMRAIDCEIR TILRVIIRKKDKAIKNGEAISDDLLGLLLESNMRESNGKADLGMSTEEIIQECKLFYF AGMETTSVLLTWTLILLSMHPEWQEKARDEVLYHFGRTTPDFEHLSRLKIVTMILYEV LRLYPPITILTRRTYKAMELGGIKYPAGVNLMLPILFIHHDPNLWGKDASEFNPERFA DGISNAAKHPGSFFPFGGGPRICIGQNFALLEAKMALSTILQHFSLELSPSYTHAPYT VITLHPQHGAQIRMKKI* >Lolium rigidum clone Lol-62 AF321867 77% to 72A18 (rice) MATRALQMLREASPWSLAGAAAAMALLWLAAWIVEWAWWTPRRL RRALQAQGLRGTQYRLFTGDVPENARLNREARSKPLPLGSHDIIQRVQPMFSNVIKEN GKFAFTWFGPTPRVMIPDPELVREVLSNKFGHYGKQKSSRLGKLLANGLANHQGEKWA KHRRILNPAFHNEKIKRMLPVFATCCEEMITRWDNSMSTQGSSEIDIWPEFQNLTGDV ISRTAFGSNYQEGMKIFQLQGELAERLIMAFQTIFIPGYWFLPTKNNKRMRAIDCEIR TILRGIIGKKDKAIKNGEAISDDLLGLLLESNMRESNGKADLEMSTEEIIQECKLFYF AGMETTSVLLTWTLILLSMHPEWQEKARDEVLYHFGRTTPDFEHLSRLKIVTMILYEV LRLYPPITILTRRTYKAMELGGIKYPAGVNLMLPILFIHHDPNLWGKDASEFNPERFA DGISNAAKHPGSFFPFGGGPRICIGQNFALLEAKMALSTILQHFSLELSPSYTHAPYT VITLHPQHGAQIRIKKI* >Lolium rigidum clone Lol-22 AF321866 78% to 72A18 (rice) MATRALQMLGEASPWNLACAAAAMALLWLAAWILEWAWWTPRRL GRALEAQGLKGTRYRLFTGDVPENARLNKEARSKPLPLGSHDIIPRVQPMISNAIKEN GKLSFTWFGPEPRVTILDPESVREILSNKFGHYGKPRSSRFGKLLANGLVNHQGEKWA KHRRILNPAFHHEKIKRMLPVFSACSEEMITRWENSMSSQGVSEVDVWPEFQNLTGDV ISRTAFGSSYQEGTKIFQLQGEQAERLMQAFQTLFIPGYWFLPTKNNRRMRAIDREIC TILRGIIEKKDRAIKSGEASSDDLLGLLLESNRRESNGKANLGMSTEDIIEECKLFYF AGMETTSVLLTWTLIVLSMHPEWQEQARKEVLHHFGRTTPDFENLSRLKIVTMVLYEV LRLYPPAIFVTRRTYKAMELGGITYPAGVNLMLPILFIHHDPNIWGKDASEFNPQRFA DGISNAVKNPAAFFPFGGGPRICIGQNFALLEAKMALSTILQRFSFELSPSYTHSPYT VLTLHPQHGAPIVLRKI* >Lolium rigidum clone Lol-2 AF321865 45% to 89A3 (Arabidopsis) PLFLLGSLALAVSLLVLLGHGRGKTRLPPGPPALLFLAKFVALR RSIFDLGPLIVDLHARHGPVISIHLFRTLIFVADRKMAHRALVQGGATFAERPPPVDP TRLFTSGGRDISSSPYGTYWRLLRRNLAAEALSPARVAHFAAARRSACDGLVSSLLRE QERQGQEEAVTLRPFLRRAMFELLVFMCFGARLEREAMDEVEELQHQVLVAFTSFPVF AFFPAVTKRLFRRRWAEYLAVRRRHEEVFVPLIHAERAGGDPPCYAESLLAVRMADDV RLTDAEMVSLCSEFLNGGTDTTVTLLEWIMAELVNHPDVQAKVYEEVRANPELNDLQG MGMPYLKAVVMEGLRLHPPGHFLLPHGVQHSGDGGVEIGGYMVPKGAEVNFLVAEIGR DETVWTAAREFRPERFLEGAEGHGVDITGSREIKMMPFGAGRRMCPGYSLGMHHAEYF VARMVMDVEWRPPVEGEEVDMAETLDFTTVIKHPLRARLFARTNSHNA* >Lolium rigidum clone Lol-5-v AF321864 40% to 71B23 (Arab.) MVMEMEPSSATLFFLSVVTLVILVSLVSRKPSSKLRRPPGPRDL PFVGSLHHLLTSQPHVALRNLAKTHGPVMRLRLGQVEAVVVSSSAAAQEVLRDNDLSF ASRPNLLATEIMCYGNLDVAFAPYGAYWRALKKLCVLELLSSRKVRQFKPVRDSETMS LIKEICAAGKGGKPVKLRSLLVKCTSSVTGKATFGERCDIDLREQFMAAMEVAQIHSS GFCVGDLFPSLGFLDVATGMRYQLWRARQQLDNVVDKIISDCEARREEKKSTPTGDED LLSLMLRIRDEGGSDFPIETINIKAIIVDLFTAGTETTSSTAEFVMSELMRNPKVMKK AQEEVWRMMENKSPQDHENHMDELPYLRMVIKETMRLHPVVPLLLPRLCRESCHVGGF EITKGTRVIINAWALATSPENWNEPEEFRPERFEDSVVVNDKGTQFKLMPFGGGRRMC PGDGFALATLELMVARLLYYFEWSLPDGMRPDELDMDVKVGTTSRRRNELRVVASPCR IPIEI* >Lolium rigidum clone Lol-3-k AF321863 42% to 71B37 (Arab.) MNDDLVYVCSALAVSILAIAVVQLLKARQRLPPGPLNLPVIGSA HRLVNALPHRAMRDLAGVHGPLMYLRVGQVPLVVVTSKEVAREVLKTHDAIFATRPKL MAGDIVAYGSTDLLFCSTPGDYFRKLRRLCVQEILSNDRIRSYQDIREDEVRSLVEDI RAAGPSAPVDLSRKIYKLTNGIVSRAAFGMKSSKAEDFVAAIKHSFVYSTGFSIADLF PGFTGILSFLTGQRRTLEGVRDTIDGILEEIINEREQILKSGRSTASEKNLVEVLLGL QGNEDFGFPITRSTVKAVILDIFAGGTETSGTSMEWAMSELMANPKVMGKLQGEIRAA FGDKEFISEADLRASGGVMKYLGLVIKETFRLHPPAPILVPRESTEACEINGYVIPAK TRVVINSWAIMRDPRYWEDAEEFRPERFEGGRMDFLGGNFEYTPFGSGRRMCPGYNYG MASMELTLVQLLHSFDWSLPDGVDQLDMTEIVSLSLTRKTHLMLRAAPHRAPLPSS* >Lolium rigidum clone Lol-31-j AF321862 44% to 714A1 (Arab.) MEKLHLAVLVLPVLLGLALLYICDIVWLRPERIRRRWRKQGVRG PRPTLLYGNTQEMKKIRQDLAPAQKQDTNNYVYTLFPHLLVWTKTYGSVFLYSIGAVD ILYISDPAMVKDMSHCTSSQLGKPIYLQKSRKPLFGEGILISNGDIWAFERKIIAPEL FMEKIKVMIGLIVEASVPLLEAWESMLDNSGGIREIDVDGYMRNFSADVIARACFGSD FATGEEIFSKLRQLQKAISQQDSLVGLSALWKYLPTKSNKEMRELEQEVRLLILDVAR REGSSSSNSNIYMHTTHNDLLRSIVDGAQQCPSYPGTAEDFIVDNCKNMYFGGHETTA ITATWCLMLLATHPEWQDRARAEALDVCCGDTAIDFDVLRRLKTITMVIQETVRLFPP ASLMVREALTDVKLGGLNVPQGTIIQAGIAVVHLDRDIWGQDAGEFRPDRFMNGAAAA CKPAHMYMPFGHGPRSCPGQHLAVVELKVLLVRLLSKYSFSPSPGYRHAPLFRLTIEP GFGMPLVVTRLP* >Lolium rigidum clone Lol-31-b AF321861 43% to 714A1 (Arab.) MEELHLLVLVLSFLLGLALLYICEILWLRPERARKRWRKQGVRG PRPTLLSGNTQEMKRIRQDLPPAQKQDINSYTHTIFPHLLLWRETYGSVFLYSTGAVD ILYVSDPGMVKDVIHCTSSELGRPIYLQKSRKPLFGDGILMSSGDIWAYERKVIAPEL FMEKIKVMVGLIVEASVPLLEAWESMLDNSGGVTEIDVDGYMRNFSADVIARACFGSD FATGEEIFSKLRQLQKAISQQDTLVGLSALWKYLPTKSNKEMRELQREVRLLILDIAS RGNSSNSNNSSGCTHTTHNDLMRSIVDGAHQCPSYSGTAEDFIVDNCKNIYFAGHETT AITATWCLMLLATHPDWQDRARAEALEACSGGTAIDFNTLRQLKTITMVIQETMRLYP PASSTMREALMDIKLGGLDVPQGTIIQTPIAVLHLDRDVWGQDAGEFRPDRFANGAAA ACKPGHMYLPFGHGPRICPGQHLAMVELKVVLVHLLSKFAFSPSPGYRHAPLFRLTIE PGFGMPLVVTKLS* >Lolium rigidum clone Lol-20-f AF321860 42% to 71B37 (Arab.) MDELFLLSVLLAAAAVALLQVLKVALNPVSDRAPPGPWKLPVIG SMHHLVNVLPHRALKDLADVHGPLMMLQMGQTPLVVASSKETARLVLKTHDTNFATRP KLLAGEIVGYEWSDIVFSPSGDYWRKLRQLCVAEILSPKRVLSFCHIREEEVGIRVEQ IRAAGLSTPVNLSVMFHSITNSIVARATFGKKRKNAAEFMAAIKSGVGLASGFNIPDL FPTLTTVLATITGMKRSLHGIHTTVDTILEEIINERNIARAEKIKAGATENVDENLTD VLIGLQGKGGFGFHLDNSKIKAIILDMFAGGTGTSASAMEWGMSELMRNPAVMEKLQG QIREAFKGKTVVTEANLQASELRYLKLVIKEALRLHPPAPLLVPRESIDMCELEGYTI PAKSRVVINAWAIGRDPRYWDDAEEFQPERFEDGTVDFTGSNYEFLPFGAGRRMCPGF NYGLASMELALVTLLYHFDWSLPEGVAEVDMEEAPGLGVRRRTPLMLLATPFVPVVA* >Lolium rigidum clone Lol-20-d AF321859 42% to 71B37 (Arab.) MDELFLLSVLLAAAAVALLQVLKVALNPVSDRAPPGPWKLPVIG SMHHLVNVLPHRALKDLADVHGPLMMLQMGQTPLVVASSKETARLVLKTHDTNFATRP KLLAGEIVGYEWSDIVFSPSGDYWRKLRQLCVAEILSPKRVLSFCHIREEEVGIRVEQ IRAAGPSTPVNLSVMFHSITNSIVARATFGKKRKNAAEFMAAIKSGVGLASGFNIPDL FPTLTTVLATITGMKRSLHGIHTSHTTVDTILEEIINERNIARAEKIKAGATENVDEN LTDVLIGLQGKGGFGFHLDNSKIKAIILDMFAGGTGTSASAMEWGMSELMRNPAVMEK LQGQIREAFKGKTVVTEANLQASGLRYLKLVIKEALRLHPPAPLLVPRESIDVCELEG YTIPAKSRVVINAWAIGRDPRYWDDAEEFQPERFEDGTVDFTGSNYEFLPFGAGRRMC PGFNYGLASMELALVTLLYHFDWSLPEGVAEVDMEEAPGLGVRRRTPLMLLATPFVPV VA* >Lolium rigidum clone Lol-BXH AF321858 55% to AP003909 58316-56692 (rice) 39% to 71B26 (Arab.) MNLEVAFLHEYASPRALVVAVVLVLVAVHLARSTSRAERLRRKL PCPRFTLPVIGHLHLIGSLPHVGLRDLARKHGPDVMLLRLGAVPTLIVSSPSAARAVL RTHDHVFASRPDTVVGDILFVGSTNVGHSPYGEYWRQVRKIITTHVLTAKKIRANLPY REQEARLALASVREAAAAGTAVDLTHLFSHFAHDMVSQAVAGRIHREDRWGKLFHDLF VGNGQLLGGFNLDDCFPSLARLGIGSANIAKQRKRWDDLLDEVIDRHTSTPMEKGDEP DFIDVMLSVQDEYKLTRNNIKSILMDMFQAGTDTTFIWLDYAMAELARAPQVMAKLQA EVRRCSTTNQLLTQEDLSSMSYLKAVMKETMRLHPPGPLLLPHASIADCEVEGYVVPA GTRVIINVWAIGRHASSWERAEEFVPERFLEGSVDANSDFYGNDFRLLPFGSGRRMCP GINFATLTFEIILANLIYHFDWELPEGSPGVDMTEAFGMDVHRKENLLLVPRVAKIV* >Lolium rigidum clone Lol-FHH-y AF321857 78% to AC084282 (rice) 44% to 81D5 (Arab.) MDKAYIAILSCAFLFLVHYVLGKVSDGRRGKKGAVQLPPSPPAV PFLGHLHLVDKPIHATMCRLAARLGPVFSLRLGSRRAVVVSSSECARECFTEHDVTFA NRPKFPSQLLVSFNGTALVTSSYGPHWRNLRRVATVQLLSAHRVACMSGVIAAEVRAM ARRLFHAAEASPDGAARVQLKRRLFELSLSVLMETIAQTKATRSEADADTDMSVEAQE FKEVVDKLIPHLGAANMWDYLPVMRWFDVFGVRNKILHAVSRRDAFLRRLIDAERRRL ADGGSDGDKKSMIAVLLTLQKTEPKVYTDTMITALCANLFGAGTETTSTTTEWAMSLL LNHPAALKKAQAEIDASVGTSRLVSVDDVPSLAYLQCIVNETLRLYPAAPLLLPHESS ADCKVGGYNVPADTMLIVNAYAIHRDPAAWEHPLVFRPERFEDGKAEGLFMIPFGMGR RRCPGETLALRTIGMVLATLVQCFDWEPVDGVNVDMTEGGGFTIPKAVPLEAVCRPRA VMRDVLQSI* >Lolium rigidum clone Lol-FHH-t AF321856 78% to AC084282 (rice) 44% to 81D5 (Arab.) MDKAYIAILSCAFLFLVHYVLGKVSDGRRGKKGAVQLPPSPPAV PFLGHLHLVDKPIHATMCRLAARLGPVFSLRLGSRRAVVVSSSECARECFTEHDVTFA NRPKFPSQLLVSFNGTALVTSSYGPHWRNLRRVATVQLLSAHRVACMSGVIAAEVRAM ARRLFHATEASPDGAARVQLKRRLFELSLSVLMETIAQTKATRSEADADTDMSVEAQE FKEVVDKLIPHLGAANMWDYLPVMRWFDVFGVRNKILHAVSRRDAFLRRLIDAERRRL ADGGSDGDKKSMIAVLLTLQKTEPKVYTDTMITALCANLFGAGTETTSTTTEWAMSLL LNHPAALKKAQAEIDASVGTSRLVSVDDVPSLAYLQCIVSETLRLYPAAPLLLPHESS ADCKVGGYNVPADTMLIVNAYAIHRDPAAWEDPLEFRPERFEDGKAEGLFMIPFGMGR RRCPGETLALRTIGMVLATLVQCFDWEPVDGVKVDMTEGGGFTIPKAVPLEAVCRPRA VMRDVLQNL* >Lolium rigidum clone Lol-FHH-v AF321855 78% to AC084282 (rice) 44% to 81D5 (Arab.) MDKAYIAILSSAFLFLVHYVLGKVSDGRRGKKGAVQLPPSPPAV PFLGHLHLVEKPIHATMCRLAARLGPVFSLRLGSRRAVVVSSSECARECFTEHDVTFA NRPKFPSQLLVSFNGTALVTSSYGPHWRNLRRVATVQLLSAHRVTCMSGVIAAEVRAM ARRLFHAAEASPDGAARVQLKRRLFELSLSVLMETIAQTKATRSEADADTDMSLEAQE FKEVVDKLIPHLGAANMWDYLPVMRWFDVFGVRSKILHAVSRRDAFLRRLINAERRRL ADGGSDGDKKSMIAVLLTLQKTEPKVYTDTMITALCANLFGAGTETTSTTTEWAMSLL LNHPAALKKAQAEIDASVGTSRLVSVDDVPSLAYLQCIVSETLRLYPAAPLLLPHESS ADCKVGGYNVPADTMLIVNAYAIHRDPAAWEDPLEFKPERFEDGKAEGLFMIPFGMGR RRCPGETLALRTIGMVLATLVQCFDWEPVDGVKVDMTEGGGFTIPKAVPLEAVCRPRV VMRDVLQNL*