ࡱ> >@= %bjbjв 26к_к_ &&&8^r4&3jjj$!jjjjjjjjjjjjjjaaGjj03j j jj ~ jjjjjjjjjjjj3jjjj jjjjjjjjj X : Leishmania major and Leishmania infantum P450s The Leishmania major genome project is not finished. These four genes are a sample found at Genbank and Sanger. >CYP51E1 LM7_11c.1.Contig2 L. major Friedlin chromosome 7_11c GenEMBL AZ048391 76% to T. brucei CYP51E1 123423 MIGEFFLLLTAGLALYGWYFCKSFNTTRPTDPPVVHGAMPFVGHIIQFGKDPLDFMLNAK 123244 123243 KKYGGVFTMNICGNRVTVVGDVHQHNKFFTPRNEILSPREVYSFMVPVFGEGVAYAAPY 123067 123066 PRMREQLNFLAEELTVAKFQNFAPSIQHEVRKFMKANWNKDEGEINILDDCSAMIINTAC 122887 122886 QCLFGEDLRKRLDARQFAQLLAKMESCLIPAAVFLPWILKLPLPQSYRCRDARAELQDI 122710 122709 LSEIIIAREKEEAQKDSNTSDLLASLLGAVYRDGTRMSQHEVCGMIVAAMFAGQHTSTIT 122530 122529 TTWSLLHLMDPRNKRHLAKLHQEIDEFPAQLNYDNVMEEMPFAEQCARESIRRDPPLIML 122350 122349 MRKVLKPVQVGKCVVPEGDIIACSPLLSHQDEEAFPNPREWNPERNMKLVDGAFCGFGAG 122170 122169 VHKCIGEKFGLLQVKTVLATVLRDYDFELLGPLPEPNYHTMVVGPTASQCRVKYIRKKA 121993 121992 AA 121987 >CYP51E1 Contig3290 Leishmania infantum 8620 MIGELLLLLAAGLALYGWYFCKSFNTTRPTDPPVVHGTTPFVGHIIQFGKDPLGFMLKAK 8799 8800 KKYGGIFTMNICGNRITVVGDVHQHSKFFTPRNEILSPREVYSFMVPVFGEGVAYAAPYP 8979 8980 RMREQLNFLAEELTVAKFQNFAPSIQHEVRKFMKANWNKDEGEINILDDCSAMIINTACQ 9159 9160 CLFGEDLRKRLDARQFAQLLAKMESCLIPAAVFLPWILKLPLPQSYRCRDARAELQDILS 9339 9340 EIIIAREKEEAQKDTNTSDLLAGLLGAVYRDGTRMSQHEVCGMIVAAMFAGQHTSTITTT 9519 9520 WSLLHLMDPRNKRHLAKLHQEIDEFPAQLNYDNVMEEMPFAEQCARESIRRDPPLVMLMR 9699 9700 KVLKPVQVGKYVVPEGDIIACSPLLSHQDEEAFPNPREWNPERNMKLVDGAFCGFGAGVH 9879 9880 KCIGEKFGLLQVKTVLATVLRDYDFELLGPLPEPNYHTMVVGPTASQCRVKYIKKKKAA 10056 &&&&&&&&&&&&&&&&&&& >AC125412 AC125412.2 Leishmania major chromosome 27 clone LB01811 strain 59% to the Trypanosoma brucei seq Tb03.27F10.920 MAANVLQSYIVAALHSAATQLPSSVQPYAMMLTREDMVSTTLATAIATA 18369 VILYTVITVVLPVLRMDFYLSKLPTIKNSIPFLGHALLLAGPSPWSKMSNWSLYPEKNLP 18190 18189 QKKKSVDGPQTSRLVTYNVAGMRVIYINEPRLLRRVLLTHQRNYRKALAAAYKHFMCLLG 18010 18009 TGLVTSEDEQWKKGRLLLSHAMRIDILDSVPEMAMKAVDRILLKLDAVDAKNPSVDLNEE 17830 17829 YRHMTLQVISESALSLSAEESDRIFPALYLPIVHECNKRVWAPWRAYMPFLHGSRMRNRC 17650 17649 LSELNKVLRDIICRRWEQRNDSNYTAKPDILALCISQIDRIDEKMIVGLIDDVKTILLAG 17470 17469 HETSAALLTFATYEVLRHPEIRQKILEEATRLFDPARCTCTVQTRYGPRGVPALNDVRS 17293 17292 LVWTPAVLRETLRRHSVVPLVMRYAAKDDVWPAADTGLDADVRIPAGCTIAVGIEGVHNN 17113 17112 PDVWNKPEVFDPTRFIDAEIANDTNYLNQSTKDVKFAKKIDPYAFIPFINGPRNCLGQHL 16933 16932 SMIETQVALAYMVLSYDLTIYRDPSYKGDVAAYEDAVGRHHDFIIPQVPHDGLKVWGT 16759 16758 PNKLFM* 16738 >Contig3226 Leishmania infantum ortholog to AC125412 20 aa diffs 6459 MAANALHSHIVAALHNAATKLPSSVQPYAMLLTREDMVSTTLATAIATAVILYTVITVVL 6280 6279 PVLRMDFYLSKLPTIKNGIPFLGHALLLAGPSPWSKMSNWSLYPEKNLPQKKKSADGPQT 6100 6099 SRLVTYNVAGMRVIYINEPRLLRRVLLTHQRNYRKALAAAYKHFMCLLGTGLVTSEDEQW 5920 5919 KKGRLLLSHAMRIDILDSVPEMAMKAVDRILLKLDAVDAKNPSVDLNEEYRHMTLQVISE 5740 5739 SALSLSAEESDRIFPALYLPIVHECNKRVWAPWRAYMPFLQGSRVRNHCLSELNKVLRNI 5560 5559 ICRRWEQRNDSNCTGKPDILALCISQIDRMDEKMIVGLIDDVKTILLAGHETSAALLTFA 5380 5379 TYEVLRHPEIRQKILEEATRLFDPARCTRTVQTRYGPRGVPAVNDVRDLVWTPAVLRETL 5200 5199 RRHSVVPLVMRYAAKDDVWPAADTGLDADVRIPAGCTIAVGIEGVHNNPDVWNKPEVFDP 5020 5019 TRFIDAEIANDTNYLNQSTKDVKFAKKIDPYAFIPFINGPRNCLGQHLSMIETQVALAYM 4840 4839 VLNYDLTIYRDPSYKGDAAAYEDAVGRHHDFIIPQVPHDGLKVWGTPNKLFM 4684 &&&&&&&&&&&&&&&&&&&&& >LM34.1.Contig35 L. major Friedlin chromosome 34 unfinished whole chromosome 145491 MTPTVSPVQAAAAIATVGFLAYATTRMLQALYSAPPNIPEPAIP PNSEDGLVWSVIKRVLYRHFYVVRKGDPLKTLQRWCVEFDYKPFVMKIFFRPHVVLSSPV DIEHVLLRADTKFYKDTGYDIVRIVVGRVGLLAVGNKAQHAVHRRILMPIFRSQNIRGVA NEIIRMHALRMMGGLFHLIQCGGEQDAVVNLSDHVFRMALSAIGEAAFRASRSESLRVRG HFDVMMKMSRVNYFC 146208 PYLKSSAQRNARNTLKEMSVELLDKNMQINQIGTRRCVMDALIDELYVHFSMDDV 146372 146373 LDHVVTFLFAGHDTVSHTLEFLFALLGTNTEVQERLYEALEDLMPSICTCPTVQELME 146546 146547 CDYLVAIVKEVLRMYPAAPIIYRDAAEDVYLPGSAVVIPKGMTVVITLSALQRNTHVYGD 146726 146727 DVDVFRPERWLGEEGEALRKRCGRCGYIPFSCGKRSCIGQELGYLELLVVTALMGRHLK 146903 146904 MELVGKFPEARYNITIAVSHSVSMRITARDGIPVSEVYERIANVLDLNDEDAGSARNVTRGV* 147092 >Contig2424 Leishmania infantum ortholog to LM34.1.Contig35, 13 aa diffs 1504 MAPTVSPVQAAAAIATVGYLAYATTRMLQALYSAPPNMPEPAIPPNSEDGLVWSVVKRVL 1325 1324 YRHFYVVRKGDPLKTLQRWCAEFDYKPFVMKIFFRPHVVLSSPVDIEHVLLRADTKFYKD 1145 1144 TGYDIVRIVVGRVGLLAVGNKAQHAIHRRILMPIFRSQNIRGVANEIIRMHALRMMGGLF 965 964 DLIQCGGEQDAVVNLSDHVFRMALSAIGEAAFRASRGESLRVRGHFDVMMRMSRVNYFCP 785 784 YLKSSAQRNARNTLKEMCVELLDKNMQINQIGTRRCVMDALIDELYVHFSMDDVLDHVVT 605 604 FLFAGHDTVSHTLEFLFALLGTNTEVQERLYEALEDLMPSICTCPTVQELMECDYLVAIV 425 424 KEVLRMYPAAPIIYRDAAEDVYLPGPAVVIPKGMTVVITLSALQRNTHVYGDDVDVFRPE 245 244 RWLGEEGEALRKRCGRCGYIPFSCGKRSCIGQELGYLELLVVTALLGRHLKMELVGEFPE 65 64 ARYNITIAVSHSVSMRITARD 2 (missing 32 aa) &&&&&&&&&&&&&&&&&&&&&& >LM30.1.Contig3 L. major Friedlin chromosome 30 unfinished whole chromosome Note this sequence matches the third seq fragment of T. cruzi 84% this seq is 51% identical to Chlamydomonas scaffold 690 52% to Choanoflagellate P450 78627 MAAFSRLLGVQLPYVMEVVIFLALAYATAYVLTNIMFASIHKFKMAGPLTAIPVLGGVVGMIRDPYSFWERQR 78845 78846 QYEPHGYSWMAILTQFVVFVTRADLCHKIFATNGEDTLTLQLHPNGKLILGDNNIAFQSG 79025 79026 PGHKALRSSFMNLFTTKALSLYLPIQERLIHEHLSRWVRDYPWGGKPEEMRTHIRELN 79199 79200 CETSQTVFLGEHLHNHTEFTHNYNIITRGFLSAPLYLPGTPLYKAVQARKLTM 79358 79359 VELQAAVRRSKARMAKPDAEPHCLLDFWTATVLEKIKEAEEEGGDAPAYSSDHAMADTIL 79538 79539 DFLFASQDASTASLTMITATMADHPEILERVRKEQARLRPNNEPLTYDLVQEMTFT 79706 79707 RQCVMEQLRLFPPAPMVPMKVHGDFQLDEKTVVRKGSMIIPSLVACCREGFTNPDTYD 79880 79881 PDRMGPERQEDRKFAKQFIPFGVGPHRCVGYNYAINHLTVYLALIAHHVEWQRTRTPD 80054 80055 SDKILYLPTLYPHDCLQTWRYREGIEPTAEKV* 80153 >Contig2309 Leishmania infantum ortholog to LM30 above (10 aa diffs) 430 MAAFSRLLGVQLPYVVEVVIFLALAYATAYVLTSIMFARIHKFKMAGPLTAIPVLGGVVT 251 250 MIRDPYSFWERQRQYEPHGYSWMAILTQFVVFVTRADLCHKIFTTNGEDTLTLQLHPNGK 71 70 LILGDNNIAFQSGPGHKALRSSF 2 (amino acid 143) 141 aa gap >Contig3659 starting at amino acid 285 3 EEGGDAPAYSSDHAMADTILDFLFASQDASTASLTMITATMADHPEILERVRKEQARLRP 182 183 NNEPLTYELVQEMTFTRQCVMEQLRLFPPAPMVPMKVHSDFQLDEKTVVPKGSMIIPSLV 362 363 ACCREGFTNPDTYDPDRMGPERQEDRKFAKQFIPFGVGPHRCVGYNYAINHLTVYLALIA 542 543 HHVRWQRTRTPDSDKILYLPTLYPHDCLQTWRYREGMEPKAEKV 674 the best matches to this seq in Fungi are CYP61 sequences CYP61A3 S. pombe Z98974 join(10345..10419,10514..12001)... 598 7.3e-62 1 CYP61A2 C.albicans AL033396 comp(39932..41485) gene="Ca... 509 2.0e-52 1 Yeast CYP61A1 Z49211 Z71257 466 7.1e-48 1 CYP51 yeast M15663 U10555 169 3.1e-17 2 in Dictyostelim CYP524A1 Seq 91 complete seq 25% to 10, 42 468 aa 774 1.1e-80 1 CYP508A3 seq 21+67+72+82+87 57% to 508A1 458aa 194 4.9e-16 1 in Chlamydomonas 21. Scaffold 690 10 EXONS 43% to 710A1 exon 1 predicted b... 1178 1.2e-123 1 2. scaf 846 BI528139 33% to 707A2 possible 85 clan membe... 211 4.5e-18 1 in rice CYP710s AP002093d $F CYP710A8 CDS complement(143977..145503... 844 3.4e-87 1 AP002092c $F CYP710A7 CDS complement(80797..82311) ... 837 1.9e-86 1 AP002092b $F CYP710A6 CDS complement(60811..62349) ... 827 2.1e-85 1 AP002092a $F CYP710A5 CDS complement(54602..56137) ... 789 2.3e-81 1 AP004162.1 $F CYP707A6 chr 8 clone OJ1320_D12, = AY0... 293 2.4e-26 2 in Arabidopsis CYP710A3 847 1.0e-87 1 CYP710A4 847 1.0e-87 1 CYP710A1 830 6.4e-86 1 CYP710A2 801 7.6e-83 1 CYP88A4 280 5.2e-25 1     /012 X 7  b s t + r G 4ek/kx Rk+?@kWk,ks@AWXk%b%L%/0y%M%ehFhFB* ph`012 X 7  b s t + r G ed|ed| 4e/x R+?@W,s@AWXed|XbL/0yMe=ed|e%=%+tFGHEPwF{| V !W!!!! "Y"Z"j""" #Z##### $Z$$$$J%%%%%%%%%%%%%%%%%%%%%%%%%%%%%h!_jhFUhF^+tFGHEPwF{| V !W!ed|W!!!! 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