Revised conifer P450s New Jan. 6, 2004

311 conifer P450 accession numbers New Jan. 6, 2004

These are the old Pinus sequences.  Please see the links above for 
New data, including the sequences below.

74 P450 ESTs from Pinus 

last modified Oct. 11, 1999 more ESTs added 2/19/02 (not exhaustive searches)

4 full length sequences are now available from mRNAs or assembled from ESTs

CYP73A20 pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA

CYP73A23 sequence assembled from ESTs AI812813, AA556941, AI812771, AI812842, 
AI919827, AW495794.1 BE762259 BF517288 BE762275
MGSEVLAQGSALLEKSLIALLVVVVGAVLVNKLRGRKLKLPPGPFALPIFGNWLQVGDDLNHRNLSDLAK 
KYGKIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKM
RRIMTVPFFTNKVVQHYRFAWEDEISRV
VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA
QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK
CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT
TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK
NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL
VQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA

CYP78A4 AF049067 Pinus radiata (Monterey pine)
MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPL
YIALLIVAVCAVLVSWLSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLPHRK
LESLARLHGAKQLMSFSLGCTPAVITSDPEVARELLTSPHFANRPLKQSAQQLLFGRA
IGFAPNGGYWRLLRRIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVR
RHLQGAALNNIMGSVFGRRFDMSHENEEVKKLREMVDEGFQLLGAFNWADHLPWLRPL
DPLRIHARCARLVPRVTTFVSNIIEQHRREEQRRESGDQCDFVDVLLSLQGEDKLDEE
DMIAVLWEMIFRGTDTTALLTEWTMAELVLHPEAQKKAQAELDAVVGHDRSVKDSDIP
KLPYIQAVVKEALRMHPPGPLLSWARLSTEDVNMGDGMCVPAGTTAMVNMWSITHDPN
IWESPYEFRPERFVVFEGGEEVDVRGNDLRLAPFGAGRRVCPGKALGLATVNLWVAKL
LHHFEWLPHAEHPVDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD

CYP98A15  AY064170 Pinus taeda p-coumarate 3-hydroxylase
MSVPEMGPFGVVVTGIVAIAIVYKLVQRWRFKLPPGPRPWPVVG
NLLQIEPVRFRCFWDWSKKYGPIMSVWFGSTLNVVVSNTELAKEVLKEHDQQLADRPR
SRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVAAMVESI
FNDCGKQEGIGKPLVVKRYLSGVAFNNITRPAFGKRFVNEEGKMDPQGVEFKEIVATG
LKLGASLTMAEHIPYLRWMFPLEEGAFAKHGARRDNVTKAIMEEHTLARQTSGAKQHF
VDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEI
DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGS
NVHVNVWAIARDPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGI
NLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRLPDHLYKR
QPLN


BF610290.1 CYP51 N-term
BF517195.1 CYP51 C-term
AW984874.1 CYP51 C-term
AW626637.1 CYP51 C-term
AW290673.1 84% to 51A2 C-term
AW043075 71D like 55% to 71D16
AI919813 84 like 53% identical to CYP84
AW010433 84 like 51% to CYP84
AI812674 73A20
AI920132 73A20
AI812813 73A23
AA556941 73A23
AI812771 73A23
AI812842 73A23
AI919827 73A23
AW042602 73A23
BF610527.1 73A23
BF609655.1 73A23
BG275633.1 73A23
BE187484.1 73A23
BE643861.1 73A23
AW290024.1 73A23
AW495794.1 73A23
AA220912 extreme C-term like 73A20 might be accidental match
AW056850 75A like 65% to 75A3
AW043335 75A like 55% identical to CYP75A3 53% identical to CYP98A1
AW870094.1 60% to 76C2
AW043205 77A  61% to 77A1
AI812861 77A  53% to 77A1 N-term
AA220898 78A4
AA220888 78A4
AA220889 78A4
AA220911 78A4
AI813156 78A 55% to 78A6
BM158467.1 68% to 78A5
AA556173 98A 80% to 98A2
BG039994.1 74% to 98A3
AW888136.1 85% to 98A3
BG625848.1 71% to 98A3
BM158852.1 57% to 98A3
BE187393.1 98A C-term
BG040095.1 98A N-term
BG039083.1 98A mid
AI920165 704 like 58% to 704A2 86 clan
AI813004 704 like, same seq as AI920165 86 clan
AW010774 52% to 86C1 in 86 clan
AA740068 90 like C-term 50% to 90A1 85 clan clone 9312M
AI725255 90 like C-term clone 9159r
AA739879 90 like C-term clone 9047M
AI725351 90 like N-term 40% to 90A1 clone 9312r opposite end of AA740068
     all four of these 90 like ESTs are from the same gene 
     have the first 164 amino acids and the C-terminal.  Missing about 208 aa
     in the middle.
BG318892.1 CYP90 like different from AA740068 *
BG318205.1 CYP90 like different from AA740068 *
BI202885.1 CYP90 like different from AA740068 *
BG318334.1 CYP90 like different from AA740068 *
BI644007.1 CYP90 like *
BI077317.1 CYP90 like *
AW011225.1 CYP90 like different from AA740068
BI398025.1 CYP90 like
BF609170.1 CYP90 like
BG318000.1 CYP85 clan
BE049731.1 CYP85 clan
BG040616.1 CYP85 clan
BE582456.1 CYP85 clan
BI416628.1 CYP85 clan
AW056514.1 CYP85 clan
BF610049.1 83% to 85A1
BM428379.1 63% to 85A2
AW011172.1 56% to 87A2
BF778196.1 87A2 like
BG625777.1 50% to 90B1
BG832634.1 50% to 90B1
AW225716.1 50% to 90B1
AI725152.1 CYP720 like
AW754540.1 81% to 703A2



many of these sequences are from The Pine Gene Discovery Project
Whetten,R.W., Kinlaw,C.S., Retzel,E., Sederoff,R.R.

Allona I, Quinn M, Shoop E, Swope K, Cyr SS, Carlis J, Riedl J, Retzel E, Campbell MM, 
Sederoff R, Whetten RW (1998)
Analysis of xylem formation in pine by cDNA sequencing.
Proc Natl Acad Sci U S A 95, 9693-9698.
go to abstract


CYP98A  AY064170 Pinus taeda p-coumarate 3-hydroxylase
MSVPEMGPFGVVVTGIVAIAIVYKLVQRWRFKLPPGPRPWPVVG
NLLQIEPVRFRCFWDWSKKYGPIMSVWFGSTLNVVVSNTELAKEVLKEHDQQLADRPR
SRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVAAMVESI
FNDCGKQEGIGKPLVVKRYLSGVAFNNITRPAFGKRFVNEEGKMDPQGVEFKEIVATG
LKLGASLTMAEHIPYLRWMFPLEEGAFAKHGARRDNVTKAIMEEHTLARQTSGAKQHF
VDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEI
DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGS
NVHVNVWAIARDPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGI
NLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRLPDHLYKR
QPLN

>gi|12482680|gb|BG040095.1|BG040095 NXSI_106_D11_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_106_D11 5'.
          Length = 480

 Score =  230 bits (587), Expect = 3e-61
 Identities = 116/160 (72%), Positives = 130/160 (80%)
 Frame = +1
98A N-term
Query: 70  LNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLE 129
           LNVVVS+ ELAKEVLKEHDQ+LADR R+RS E FSRNGQDLIWADYGPHYVKVRKVCTLE
Sbjct: 1   LNVVVSNTELAKEVLKEHDQQLADRPRSRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLE 180

Query: 130 LFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGK 189
           LF+PKRLE+LRPIREDEV AMVES+F DC   E   K L +++YL  VAFNNITRLAFGK
Sbjct: 181 LFSPKRLEALRPIREDEVAAMVESIFNDCGKQEGIGKPLVVKRYLSGVAFNNITRLAFGK 360

Query: 190 RFMNAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLR 229
           RF+N  G +D   +EF  IV+ GLKLGAS   AEH  +LR
Sbjct: 361 RFVNEXGKMDPXXVEFXXIVATGLKLGASPHXAEHXXYLR 480

>gi|12481668|gb|BG039083.1|BG039083 NXSI_092_E06_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_092_E06 5'.
          Length = 491
98A like mid region

Query: 135 RLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKRFMNA 194
           RLE+LRPIREDEV AMVES+F DC   E   K L +++YL  VAFNNITRLAFGKRF+N 
Sbjct: 1   RLEALRPIREDEVAAMVESIFNDCGKQEGIGKPLVVKRYLSGVAFNNITRLAFGKRFVNE 180

Query: 195 EGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWMFPADEKAFAEHGARRDRLTRAIM 254
           EG +  QG+EFK IV+ GLKLGASL++AEHIP+LRWMFP  E AFA+HGARRD +T+AIM
Sbjct: 181 EGKMXPQGVEFKXIVATGLKLGASLTMAEHIPYLRWMFPLXEGAFAKHGARRDNVTKAIM 360

Query: 255 EEHTLARQKSSGAKQHFVDALLTLQEKYDLSEDTIIGLLWDM 296
            EHTLARQ + GAKQHFVDALLTLQEKYD SE TI   LWDM
Sbjct: 361 XEHTLARQ-TXGAKQHFVDALLTLQEKYDXSEXTIXXXLWDM 483

>gi|8666577|gb|BE187393.1|BE187393 NXNV_163_D07_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
           clone NXNV_163_D07 5'.
          Length = 591

CYP98A
Query: 437 ICPGAQLGINLVQSMLGHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQAVATPRL 496
           ICPGAQLGINLVQSMLGHLLHHFVW PP+G + E+ID++ENPGLVT+M  PVQA+A PRL
Sbjct: 1   ICPGAQLGINLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRL 180

Query: 497 PSDLYKRVP 505
           P  LYKR P
Sbjct: 181 PDHLYKRQP 207



73A20 search pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA

AI812674 17G2 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
2 diffs with 73A20

Query: 1   MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN 60
           MEIMTVASLEKGLLAIFAVIVGAIFIS LKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN
Sbjct: 47  MEIMTVASLEKGLLAIFAVIVGAIFISNLKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN 226

Query: 61  LGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM 120
           LGDLA KYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM
Sbjct: 227 LGDLANKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM 406

Query: 121 VFTVYGEHWR 130
           VFTVYGEHWR
Sbjct: 407 VFTVYGEHWR 436

gb|AI920132.1|AI920132 1662 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
           f1_PL2CSUH, mRNA sequence
           Length = 615
           
 Score = 41.4 bits (95), Expect = 9e-05
 Identities = 23/29 (79%), Positives = 24/29 (82%)
 Frame = +2
73A20
Query: 478 QSKVDVTEKGGQFSLHILNHSVVVAKPIA 506
           QSKV   E+  QFSLHILNHSVVVAKPIA
Sbjct: 143 QSKVMCRERR-QFSLHILNHSVVVAKPIA 226


gb|AI812813.1|AI812813 19E5 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 489
           
 Score =  277 bits (701), Expect = 8e-76
 Identities = 133/162 (82%), Positives = 149/162 (91%), Gaps = 1/162 (0%)
 Frame = +1

Query: 161 VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSRLA 220
           V D+K R E+ST+GIVIR+RLQL+MYNIMYRMMFDRRFESEDDPLFL+LKALNGERSRLA
Sbjct: 1   VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA 180

Query: 221 QSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVTGD-K 279
           QSFEYNYGDFIP+LRPFLRGYL+IC E+KE RL LFKDYF++ERKKL ST  S  TG+ K
Sbjct: 181 QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK 360

Query: 280 CAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGL 322
           CA+DHI +A+ KGEINEDNVLYIVENINVAAIETTLWSMEWG+
Sbjct: 361 CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGI 489

73A23 sequence assembled from ESTs AI812813, AA556941, AI812771, AI812842, 
AI919827, AW495794.1 BE762259 BF517288 BE762275
MGSEVLAQGSALLEKSLIALLVVVVGAVLVNKLRGRKLKLPPGPFALPIFGNWLQVGDDLNHRNLSDLAK 
KYGKIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKM
RRIMTVPFFTNKVVQHYRFAWEDEISRV
VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA
QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK
CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT
TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK
NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL
VQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA

73A20 search pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKM
RRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA


 gb|AA556941|AA556941 783 Loblolly pine C Pinus taeda cDNA clone 7C7B
           Length = 678
           
 Score =  263 bits (666), Expect(2) = 2e-75
 Identities = 127/154 (82%), Positives = 139/154 (89%), Gaps = 1/154 (0%)
 Frame = +2

Query: 295 NEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQKIRAELDAVIGRGVPLTEPDT 354
           NEDNVLYIVENINVAAIETTLWSMEWG+AE+VNH +IQ K+RAELDAV+G GV  TEPDT
Sbjct: 2   NEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT 181

Query: 355 TKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKILVNAWFLANNPEWWE 414
           T+LPYLQAVVKETLRL MAIPLLVPH NLH AKLGGYDIPAESKILVNAW+LANNP  W+
Sbjct: 182 TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK 361

Query: 415 KPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPGIILALPILALALGRL 466
            PEEF PERF  EEK  EA+GNDF+FLPFGVGR++CPGIILALP+LAL++GRL
Sbjct: 362 NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL 515

Query: 467 VQNFELLPPPGQSKVDVTEKGGQFSLHILN 496
           V  F LLPPPGQ+K  + EKGGQ  L ILN
Sbjct: 516 VXXFHLLPPPGQTKW-IPEKGGQSPLXILN 603


gb|AI812771.1|AI812771 18H4 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 518
           
 Score =  295 bits (747), Expect = 3e-81
 Identities = 143/167 (85%), Positives = 155/167 (92%), Gaps = 1/167 (0%)
 Frame = +2

Query: 340 DAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKI 399
           DAV+G GV +TEPDTT+LPYLQAVVKETLRL MAIPLLVPHMNLH AKLGGYDIPAESKI
Sbjct: 2   DAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKI 181

Query: 400 LVNAWFLANNPEWWEKPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPGIILALPI 458
           LVNAW+LANNP  W+ PEEF PERF  EEK  EA+GNDF+FLPFGVGRRSCPGIILALP+
Sbjct: 182 LVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPL 361

Query: 459 LALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA 506
           LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNHS++VAKPIA
Sbjct: 362 LALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA 505

 gb|AI812842.1|AI812842 20F6 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 539
           
 Score =  192 bits (484), Expect = 2e-50
 Identities = 93/117 (79%), Positives = 101/117 (85%), Gaps = 1/117 (0%)
 Frame = +2

Query: 335 IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIP 394
           +RAELDAV+G GV +TEPDTT+LPYLQAVVKETLRL MAIPLLVPHMNLH AKLGGYDIP
Sbjct: 2   VRAELDAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIP 181

Query: 395 AESKILVNAWFLANNPEWWEKPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPG 451
           AESKILVNAW+LANNP  W+ PEEF PERF  EEK  EA+GNDF+FLPFG G    PG
Sbjct: 182 AESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDFKFLPFGCGEEELPG 355


 Score =  122 bits (303), Expect = 4e-29
 Identities = 58/66 (87%), Positives = 63/66 (94%)
 Frame = +3

Query: 441 PFGVGRRSCPGIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVV 500
           P  VGRRSCPGIILALP+LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNHS++
Sbjct: 324 PSVVGRRSCPGIILALPLLALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLI 503

Query: 501 VAKPIA 506
           VAKPIA
Sbjct: 504 VAKPIA 521


 gb|AI919827.1|AI919827 1371 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
           C7_PL12CSUH, mRNA sequence
           Length = 807
           
 Score =  129 bits (321), Expect = 3e-31
 Identities = 61/69 (88%), Positives = 67/69 (96%)
 Frame = +1

Query: 438 RFLPFGVGRRSCPGIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNH 497
           +FLPFGVGRRS PGIILALP+LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNH
Sbjct: 370 KFLPFGVGRRSXPGIILALPLLALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNH 549

Query: 498 SVVVAKPIA 506
           S++VAKPIA
Sbjct: 550 SLIVAKPIA 576




CYP78A4 search pinus radiata

AA220898 13 Pinus radiata somatic embryogenic library Pinus radiata cDNA
4 differences to 78A4
Query: 175 RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF 234
           RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF
Sbjct: 3   RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF 182

Query: 235 GRRFDMSHENEEVKKLREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVPRVT 293
           GRRFDMSHENEEVK+LREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVP VT
Sbjct: 183 GRRFDMSHENEEVKRLREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVPSVT 359

FVSNIIEQHRREEQRR 310
FV NIIE+HRREEQRR
FVRNIIEEHRREEQRR 409

AA220888 3 Pinus radiata somatic embryogenic library Pinus radiata cDNA
3 diffs to 78A4
Query: 1   MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW 60
           MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW
Sbjct: 73  MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW 252

Query: 61  LSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLP 99
           LSPGGCAWAGRHK+ RVAIPGPKGWPIIG LMDMSVGLP
Sbjct: 253 LSPGGCAWAGRHKKSRVAIPGPKGWPIIGILMDMSVGLP 369

AA220911 26 Pinus radiata somatic embryogenic library Pinus radiata cDNA
1 diff to 78A4
Query: 522 VDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD 553
           VDLSEVLKLSCEMARPLHCVP TRVPFAKFSD
Sbjct: 14  VDLSEVLKLSCEMARPLHCVPGTRVPFAKFSD 109

gb|AA220889|AA220889 4 Pinus radiata somatic embryogenic library Pinus radiata cDNA
           clone pPRE74D 3'
           Length = 394
           
 Score = 33.6 bits (75), Expect = 0.022
 Identities = 16/23 (69%), Positives = 17/23 (73%)
 Frame = +3

Query: 514 WLPHAEHPVDLSEVLKLSCEMAR 536
           W  HAEHPV +   LKLSCEMAR
Sbjct: 3   WFQHAEHPVIVGG-LKLSCEMAR 68

CYP98A2 search
gb|AA556173|AA556173 28 Loblolly pine N Pinus taeda cDNA clone 2N4B
           Length = 710
Score =  260 bits (657), Expect(2) = 3e-77
 Identities = 122/153 (79%), Positives = 138/153 (89%)
 Frame = +3
CYP98A subfamily
Query: 247 RDRLTRAIMAEHTEARKKSGGAKQHFVDALLTLQDKYDLSEDTIIGLLWDMITAGMDTTA 306
           RD +T+AIM EHT AR+ SG AKQHFVDALLTLQ+KYDLSEDTIIGLLWDMITAGMDTTA
Sbjct: 15  RDNVTKAIMEEHTLARQTSG-AKQHFVDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTA 191

Query: 307 ISVEWAMAELIRNPRVQQKVQEELDRVIGLERVMTEADFSNLPYLQCVTKEAMRLHPPTP 366
           I+VEWAMAEL+RNPR+ QK QEE+ RV+G +RVM E DF +LPYLQC+TKEA+RLHPPTP
Sbjct: 192 ITVEWAMAELVRNPRIXQKAQEEIXRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTP 371

Query: 367 LMLPHRANANVKVGGYDIPKGSNVHVNVWAVARDPAVWKDP
           LMLPH+A  NVK+GGYDIP GSNVHVNVWA+ARDPAVWK P
Sbjct: 372 LMLPHKATQNVKIGGYDIPXGSNVHVNVWAIARDPAVWKGP


gb|AI920165.1|AI920165 1695 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
           h2_PL3CSUH, mRNA sequence
           Length = 742
like CYP86A8
 Score = 84.3 bits (205), Expect = 1e-17
 Identities = 50/98 (51%), Positives = 61/98 (62%), Gaps = 3/98 (3%)
 Frame = +2
58% to 704A2
Query: 413 GRMKSTWGEDCLEFKPERWISQSDGGRFINH--DPFKFVAFNAGPRICLGKDLAYLQMKS 470
           GRM   W +D  EFKPERWI   DG   I+H   PFKF+AF AGPR CLGKD AYLQMK 
Sbjct: 245 GRMSYXWWDDAKEFKPERWIQ--DG---ISHPKSPFKFLAFQAGPRTCLGKDFAYLQMKI 409

Query: 471 IASAVLLRHRLTVVTGHKVEQKMSLTLFM-KYGLLVNVHER 510
           +A+ ++   +   V   +V  +  LTL M + GL V V  R
Sbjct: 410 VAAVLVRFFKFEAVKTKEVRYRTMLTLHMNEDGLNVQVTPR 532


 gb|AI813004.1|AI813004 2F2 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 594
           
 Score = 58.2 bits (138), Expect = 9e-10
 Identities = 34/73 (46%), Positives = 42/73 (56%), Gaps = 1/73 (1%)
 Frame = +1

Query: 438 GRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRHRLTVVTGHKVEQKMSLTL 497
           G F    PFK  AF AGPR CLGKD AYLQMK +A+ ++   +   V   +V  +  LTL
Sbjct: 10  GIFHPKSPFKXPAFQAGPRTCLGKDFAYLQMKIVAAVLVRXFKFEAVKTKEVRYRTMLTL 189

Query: 498 FM-KYGLLVNVHER 510
            M + GL V V  R
Sbjct: 190 HMNEDGLNVQVTPR 231


gb|AA740068|AA740068 833 PtIFG2 Pinus taeda cDNA clone 9312M 3'
           Length = 572
           
 Score = 36.0 bits (81), Expect = 0.004
 Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
 Frame = +3

Query: 443 WSITHDPNIWESPYEFRPERFVVFEGGEEVDVRGNDLRLAPFGAGRRVCPGKALGLATVN 502
           W    +P+ W+S    +    + +    + DV  N L  +PFG G R+CPG  L    + 
Sbjct: 3   WXFDCNPSRWKSS-TMKXLHLIPWRWELDQDVSNNHL-FSPFGGGARLCPGSHLARLELA 176

Query: 503 LWVAKLLHHFEWLPHA-EHP 521
            ++   +  F W   A EHP
Sbjct: 177 XFLHIFITRFRWEALADEHP 236

DTKGXXIPKGWVVFAFLLATHLDEXXXXXXXTFNPWRWELDQDVSNNHLFSPFGGGARLCPGSHLARLELA 176
LFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMRLYNRE* 236


gb|AA739879|AA739879 644 PtIFG2 Pinus taeda cDNA clone 9047M 3'
           Length = 604
           
 Score = 30.1 bits (66), Expect = 0.22
 Identities = 26/74 (35%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
 Frame = +2

Query: 397 SKILVNAWFLANNPEWWEKPE----EFIPERFLGEEKIEASGNDFRFLPFGVGRRSCPGI 452
           SK +    F A NP   + P       IP R+  ++ +    N+  F PFG G R CPG 
Sbjct: 38  SKRMGGVCFFACNPSR*KVPTMXFLHLIPWRWELDQDVS---NNHLFSPFGGGARLCPGS 208

Query: 453 ILALPILALALGRLVQNF 470
            LA    AL L   +  F
Sbjct: 209 HLARLEXALFLHIFITRFRWEALADEHP 262


AI919813 1357 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
           C1_PL11CSUH, mRNA sequence
           Length = 653
53% identical to CYP84
 Score = 67.9 bits (163), Expect = 9e-13
 Identities = 41/104 (39%), Positives = 58/104 (55%), Gaps = 7/104 (6%)
 Frame = -3

Query: 397 VARDPAVWKD-----PLEFRPERFLEEDVDMKGHDFRLLPFGSGRRVCPGAQLGINLAAS 451
           + R+P  W+D     P E   ++    ++D+   +F  +PFG+GRR CPG QLG+ +   
Sbjct: 540 IGRNPKSWEDXESSKPREVSWKKXGXAELDVNVQNFEWIPFGAGRRGCPGQQLGMLVVEX 361

Query: 452 MLGHLLHHFCWTPPEGMK-PEEIDMGEN-PGLVTYMRTPIQAVVSPRLPSH 500
            +  LLH F W  PE M   EE+DM E   GL       + AV +PRL  H
Sbjct: 360 GMAQLLHCFNWRLPEKMNGQEELDMSERFNGLTLPRAHELVAVPTPRLSVH 208


gb|AI812861.1|AI812861 20H12 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 508
           
 Score = 59.3 bits (141), Expect = 4e-10
 Identities = 35/92 (38%), Positives = 57/92 (61%), Gaps = 4/92 (4%)
 Frame = -2
53% to 77A1 N-term
Query: 4   LLIIPISLVTLWLGYTLYQRLRFK-LPPGPRPWPVVGNLYDI---KPVRFRCFAEWAQSY 59
           +L++ ISLV L L   L++  R++ LPPGP  WP++GNL  +        R   ++ + Y
Sbjct: 291 VLLLVISLVFLRL--VLWKIWRYRNLPPGPPAWPILGNLLQMGFASGAFERSVQKFHERY 118

Query: 60  GPIISVWFGSTLNVIVSNSELAKEVLKEHDQLLADR 95
           GPI ++W GS   +++++ ELA E L +   L +DR
Sbjct: 117 GPIFTMWLGSRPLLMIASQELAHEALIQKGSLFSDRP 10
probable N-terminal of a CYP77 from pine
MAILSFLQAVLLLVISLVFLRLVLWKIWRYRNLPPGP
PAWPILGNLLQMGFASGAFERSVQKFHERYGPIFTMWLGSRPLLMIASQE
LAHEALIQKGSLFSDRPPA

gb|AA220912|AA220912 27 Pinus radiata somatic embryogenic library Pinus radiata cDNA
           clone pPRE59A 3'
           Length = 508
           
 Score = 29.7 bits (65), Expect = 0.32
 Identities = 16/32 (50%), Positives = 16/32 (50%)
 Frame = +1

Query: 522 VDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD 553
           V    VLKLS E     H V  TR PF KF D
Sbjct: 64  VXXXXVLKLSXEXVXLFHXVSGTRXPFGKFXD 159

gb|AI813156.1|AI813156 23F12 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA 
sequence
           Length = 456
55% to 78A6
 Score =  143 bits (356), Expect = 2e-35
 Identities = 75/144 (52%), Positives = 102/144 (70%)
 Frame = +1

Query: 45  YIALLIVAVCAVLVSWLSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLPHRKLE 104
           ++ +L++A+  VL+SWLSPGG AW G  KR +  IPGP G PI+G+L  M   L H++L 
Sbjct: 19  FLLVLLIAMAYVLLSWLSPGGPAWGGSLKR-KPTIPGPMGLPILGNLTVMC-SLAHQRLA 192

Query: 105 SLARLHGAKQLMSFSLGCTPAVITSDPEVARELLTSPHFANRPLKQSAQQLLFGRAIGFA 164
            LA+   A++LM+FS+G T AVI S PEVA+ +L S  FA+RP+K+SA QL+F  AIGFA
Sbjct: 193 YLAQACNAQKLMAFSMGFTRAVICSQPEVAKAILNSSAFADRPIKESAYQLMFDTAIGFA 372

Query: 165 PNGGYWRLLRRIASAHLFAPRRIA 188
           P   YW+ L RIA+ HLF+  RI+
Sbjct: 373 PYSEYWKNLMRIAALHLFSVPRIS 444
complete translation 55% to 78A6
AFCNTWFLLVLLIAMAYVLLSWLSPGGPAWGGSLKRKPTIPGPMGLPILG
NLTVMCSLAHQRLAYLAQACNAQKLMAFSMGFTRAVICSQPEVAKAILNS
SAFADRPIKESAYQLMFDTAIGFAPYSEYWKNLMRIAALHLFSVPRISGL
RI

gb|AI725351.1|AI725351 1217 PtIFG2 Pinus taeda cDNA clone 9312r, mRNA sequence
           Length = 656
similar to 90A 40%

Query: 24  SKLRGKKLKLPPGPIPIPIFGN----WLQVGDDLNHRNLVDYAKKF-GDLFLLRMGQRNL 78
           S    ++L LPPG    P+ G     +  +  +   + + D  K++  D+F+  +     
Sbjct: 132 SNKXNQELHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQA 311

Query: 79  VVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRI 132
           V+ S P   K VLL +G  F +     +  +    G   + +V+G+  RK+  I
Sbjct: 312 VISSDPQFNKYVLLNEGRFFQAHYPXALKALIRDYG---LLSVHGDLQRKLHGI 464

probable first 164 amino acids of a CYP90 like P450 from pinus
MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQE
LHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG
IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRK
LHGITVNLLKFEKLKFDFMEEI

>BG318892.1 NXPV_021_B08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_021_B08 5'.
          Length = 570
CYP90 like sequence
Query: 1   MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
           MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15  MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194

Query: 61  YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
           YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374

Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEEI 164
            ALKALI DYGLLSVHGDLQRKLHGI VNLL+FE+LKFDFMEEI
Sbjct: 375 KALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEI 506

15  MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374
375 KALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEIQNLVHSTID 


>BG318205.1 NXPV_011_G08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_011_G08 5'.
          Length = 556
CYP90 like sequence
Query: 1   MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
           MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15  MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194

Query: 61  YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
           YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374

Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEE 163
            ALKALI DYGLLSVHGDLQRKLHGI  NLL FE+LKFDFMEE
Sbjct: 375 KALKALIGDYGLLSVHGDLQRKLHGIAXNLLXFERLKFDFMEE 503

>BI202885.1 NXPV_091_G09_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_091_G09 5'.
          Length = 496

Query: 1   MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
           MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15  MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194

Query: 61  YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
           YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374

Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDF 160
            ALKALI DYGLLSVHGDLQRKLHGI  NLL     K  F
Sbjct: 375 KALKALIXDYGLLSVHGDLQRKLHGIXXNLLXXXXXKXXF 494

>BG318334.1 NXPV_012_D03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_012_D03 5'.
          Length = 326

Query: 1   MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
           MADQISLLLVVFTAAVALLHLIYRWWNA RG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15  MADQISLLLVVFTAAVALLHLIYRWWNAXRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194

Query: 61  YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFN 104
           YYRSMT+NRP+QFIDDREKRYDSDIFVSHLFG  A ISS    N
Sbjct: 195 YYRSMTNNRPKQFIDDREKRYDSDIFVSHLFGXXAXISSXXXXN 326

>BI644007.1 NXPV_138_E03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_138_E03 5'.
          Length = 354

Query: 25  WWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSD 84
           WWNA  G KRT+N+ N E   PPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRY SD
Sbjct: 1   WWNAXXGQKRTNNEKNPEGXXPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYXSD 180

Query: 85  IFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAH
           IFVSHLFG QA ISSDPQFNK+VL NEGRFFQAH
Sbjct: 181 IFVSHLFGSQAXISSDPQFNKHVLQNEGRFFQAH

YPXALKALIRDYGLLSVHGDLQR 141
YP ALKALI DYGLLSVHGDLQR
YPKALKALIGDYGLLSVHGDLQR 352




New Pine ESTs 10 Sept. 99

AW043335 ST32B07 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST32B07, mRNA sequence
           Length = 593 55% identical to CYP75A3
           
 Score =  123 bits (307), Expect(2) = 1e-33
 Identities = 52/126 (41%), Positives = 82/126 (64%)
 Frame = +1

Query: 306 NAGTITMIWTMTELTRHPRVMKKLREEIRATLGPNKERITEEDLEKVEYLKLVIKETFRL 365
           +  +IT+ W M ELTRHP+++++ +EE+ + +G  +  + E DL+ + YLK V+KET RL
Sbjct: 10  DTSSITVEWAMAELTRHPKLLRRCQEELDSAVGGEQRTLKESDLQNLPYLKSVVKETLRL 189

Query: 366 HPPGPFLLPRQVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERFANTSIN 425
           HPP P L+PR       I  Y+IPKNA + ++ + + RDP  W N  EFNP+RF  +S++
Sbjct: 190 HPPVPLLIPRXAPXXXXIXXYYIPKNARLXVNAWGMQRDPSVWGNSLEFNPDRFMVSSVD 369

Query: 426 YKGQHY 431
            +G  +
Sbjct: 370 VRGSDF 387


 Score = 37.9 bits (86), Expect(2) = 1e-33
 Identities = 14/29 (48%), Positives = 24/29 (82%)
 Frame = +3

Query: 429 QHYELLPFGAGRRSCPGMTLGITILELGL 457
           + ++++PFGA RR+C GM++GI ++EL L
Sbjct: 378 ERFQVIPFGAXRRTCAGMSMGIRMVELML 464

55% identical to CYP75A3 53% identical to CYP98A1
DTSSITVEWAMAELTRHPKLLRRCQEELDSAVGGEQRTLKESDLQNLPYLKSVVKETLRL
HPPVPLLIPRXAPXXXXIXXYYIPKNARLXVNAWGMQRDPSVWGNSLEFNPDRFMVSSVD
VRGSDFQVIPFGAXRRTCAGMSMGIRMVELML


gb|AW056850.1|AW056850 ST57B04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST57B04, mRNA sequence
           Length = 438
           
 Score =  122 bits (304), Expect = 3e-28
 Identities = 53/113 (46%), Positives = 80/113 (69%)
 Frame = +2

Query: 385 GYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERFANTSINYKGQHYELLPFGAGRRSCP 444
           GY+IPKNA + ++ + I RDP  W  P EF+PERFA ++++ +G  +E++PFGAGRR C 
Sbjct: 2   GYYIPKNARLLVNAWGIQRDPDVWERPLEFDPERFAGSAVDVRGTDFEVIPFGAGRRVCA 181

Query: 445 GMTLGITILELGLLNILYYFDWSLPNGMTIKDIDMEEDGALTIAKKVPLELIP 497
           G+++GI +++L L ++L+ FDWSLP G   +++DM E   LT+ K VPL   P
Sbjct: 182 GVSMGIRMVQLMLASLLHSFDWSLPEGQLPENLDMAEAYGLTLQKAVPLLAAP 340

65% to CYP75A3
GYYIPKNARLLVNAWGIQRDPDVWERPLEFDPERFAGSAVDVRGTDFEVIPFGAGRRVCA 
GVSMGIRMVQLMLASLLHSFDWSLPEGQLPENLDMAEAYGLTLQKAVPLLAAP 

gb|AW042602.1|AW042602 ST23G12 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST23G12, mRNA sequence
           Length = 478
           
 Score =  112 bits (278), Expect = 4e-25
 Identities = 58/142 (40%), Positives = 84/142 (58%), Gaps = 4/142 (2%)
 Frame = +1

Query: 316 MTELTRHPRVMKKLREEIRATLGPNKERITEEDLEKVEYLKLVIKETFRLHPPGPFLLPR 375
           + EL  H  +  K+R E+ A LGP  + ITE D  ++ YL+ V+KET RL    P L+P 
Sbjct: 13  IAELVNHQDIQSKVRAELDAVLGPGVQ-ITEPDTTRLPYLQAVVKETLRLRMAIPLLVPH 189

Query: 376 QVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERF----ANTSINYKGQHY 431
             + D ++ GY IP  + I ++ + +  +P  W NPEEF PERF     +T  N  G  +
Sbjct: 190 MNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEAN--GNDF 363

Query: 432 ELLPFGAGRRSCPGMTLGITILELGL 457
           + LPFG  RRSCPG+ L + +L L +
Sbjct: 364 KFLPFGGWRRSCPGIILALPLLALSI 441

83% to 73A5, 79% to 73A20 (Pinus) exact to 73A23
IAELVNHQDIQSKVRAELDAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPH 
MNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDF 
KFLPFGGWRRSCPGIILALPLLALSI

gb|AW043075.1|AW043075 ST29A04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST29A04, mRNA sequence
           Length = 601
           
 Score = 93.6 bits (229), Expect = 2e-19
 Identities = 58/142 (40%), Positives = 80/142 (55%), Gaps = 1/142 (0%)
 Frame = +3

Query: 26  SKWKLPPGPTGLPIIGNLHQAWKLLHSSFHKLSLEHGPVMLLRFGVVP-MVVISSKEAAK 84
           +K +LP GP  LPIIGNLH   KL H +   LSL++GP M LR G    M+VISS + A+
Sbjct: 129 NKRRLPSGPVQLPIIGNLHLLGKLPHQALSALSLKYGPFMSLRLGSSTLMLVISSGDMAE 308

Query: 85  QVLKSRDLETCSRPKLVANGLFTRNFKDIAFAQYGEDWREMKKLVGLELFNPKKHKFFRY 144
           + LK+ D    SR    A    T N   I FA YG  WR+M+K+  L+L + K+   F+ 
Sbjct: 309 EFLKTHDQCFASRAPSTAAKCLTYNCSGIVFAPYGPYWRQMRKVCVLQLLSSKRIDSFKX 488

Query: 145 IREEEGDLLVKKLSKSSQTQTLV 167
            RE     ++  L   +QTQ ++
Sbjct: 489 TREXGVSXMI--LXIINQTQXIL 551

55% to 71D16

NKRRLPSGPVQLPIIGNLHLLGKLPHQALSALSLKYGPFMSLRLGSSTLMLVISSGDMAE
EFLKTHDQCFASRAPSTAAKCLTYNCSGIVFAPYGPYWRQMRKVCVLQLLSSKRIDSFKX
TREXGVSXMILXIINQTQXIL

gb|AW010433.1|AW010433 ST06G04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST06G04, mRNA sequence
           Length = 637
           
 Score = 88.2 bits (215), Expect = 9e-18
 Identities = 53/123 (43%), Positives = 74/123 (60%), Gaps = 8/123 (6%)
 Frame = +3

Query:     383 IQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERF---ANTSINYKG-QHYELLPFGA 438
               I GY IP+N  + ++ +AIGRDPK W + E F PERF     + ++  G Q+   L FG 
Sbjct: 6   ESCNILGYEIPQNTCVIVNLWAIGRDPKSWEDAETFKPERFMERVGSEVDANGDQNLGWLAFGT 197

Query: 439 GRRSCPGMTLGITILELGLLNILYYFDWSLP-NGMTIKDIDM---EEDGALTIAKKVPLE 494
           GRR CPG  LG+  L LG+  +L+ F+W LP + M  K+ +M   E+    T+ K   L 
Sbjct: 198 GRRRCPGEQLGMLALXLGVAQLLHCFNWRLPFDDMNGKNQEMEMTEKHNGFTLPKADELC 377

Query: 495 LIPTLPASLCI 505
            IPT P   C+
Sbjct: 378 AIPT-PRLECV 407

51% to CYP84
ILGYEIPQNTCVIVNLWAIGRDPKSWEDAETFKPERFMERVGSEVDANGDQNLGWLAFGT
GRRRCPGEQLGMLALXLGVAQLLHCFNWRLPFDDMNGKNQEMEMTEKHNGFTLPKADELC
AIPTPRLECV

gb|AW010774.1|AW010774 ST12C06 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST12C06, mRNA sequence
           Length = 491
           
 Score = 63.6 bits (152), Expect = 2e-10
 Identities = 39/147 (26%), Positives = 66/147 (44%), Gaps = 5/147 (3%)
 Frame = +2

Query: 310 ITMIWTMTELTRHPRVMKKLREEIRATL----GPNKERITEEDLEKVEYLKLVIKETFRL 365
           I + W    L +HP V + +  E+R  L    G N    T E++ +++YL   + ET RL
Sbjct: 20  IALSWLFWLLIQHPAVEENILSELRQILVSRRGSNPGGFTVEEVRQMQYLHAALSETLRL 199

Query: 366 HPPGPFLLPRQVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCW-TNPEEFNPERFANTSI 424
           +P  P      +  ++   G  + +  ++    Y++GR    W  +  EF PER+    +
Sbjct: 200 YPSLPINYKEVIEDEVLPDGARLKRGTNLIFPIYSMGRTXXIWGEDCREFKPERWLKEGV 379

Query: 425 NYKGQHYELLPFGAGRRSCPGMTLGITILELG 456
                 Y+   F A  R C G      + E+G
Sbjct: 380 FVAEGGYKYPVFSAXPRVCLGKDFAFFVHEMG 475

52% to 86C1
IALSWLFWLLIQHPAVEENILSELRQILVSRRGSNPGGFTVEEVRQMQYLHAALSETLRL 
YPSLPINYKEVIEDEVLPDGARLKRGTNLIFPIYSMGRTXXIWGEDCREFKPERWLKEGV 
FVAEGGYKYPVFSAXPRVCLGKDFAFFVHEMG 

gb|AW043205.1|AW043205 ST30E11 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST30E11, mRNA sequence
           Length = 587
           
 Score = 60.9 bits (145), Expect = 1e-09
 Identities = 39/122 (31%), Positives = 59/122 (47%), Gaps = 4/122 (3%)
 Frame = +2

Query: 19  LFKKLLPSKWKLPPGPTGLPIIGNLHQ---AWKLLHSSFHKLSLEHGPVMLLRFGVVPMV 75
           LF K  P K  LPPGP G P++GNL Q   + K        L  +  P+  L+ G   M+
Sbjct: 179 LFLKSRPPKKHLPPGPRGWPLVGNLFQVVFSGKPFIYVIRDLREKFSPIFTLKMGQKTMI 358

Query: 76  VISSKEAAKQVLKSRDLETCSRPKLVANGL-FTRNFKDIAFAQYGEDWREMKKLVGLELF 134
           +I+  + A + L        SRP      + F+ N   +  + YG  WR +++ +  EL 
Sbjct: 359 IITCPKVAHEALIENGALFASRPPDTPTKMFFSENKCSVNSSLYGSVWRXLRRNMVSELL 538

Query: 135 NPKKHK 140
           NP + K
Sbjct: 539 NPSRMK 556

61% to CYP77A1
LFLKSRPPKKHLPPGPRGWPLVGNLFQVVFSGKPFIYVIRDLREKFSPIFTLKMGQKTMI 
IITCPKVAHEALIENGALFASRPPDTPTKMFFSENKCSVNSSLYGSVWRXLRRNMVSELL 
NPSRMK 

New hits 2/19/02

>BI398025.1 NXPV_113_E11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_113_E11 5'.
          Length = 524
CYP90 like
Query: 344 LTWQDYKTMDFTQCVIDETLRLGGIAIWLMREAKEDVSYQD-YVIPKGCFVVPFLSAVHL 402
           L W DY+++ FTQCVI ETLR+G     + RE KED+  +  +VIP+G  V  FL+  HL
Sbjct: 14  LNWDDYQSLKFTQCVIHETLRVGNFGPGVFRETKEDIKTKGGFVIPRGWTVYVFLTGTHL 193

Query: 403 DESYYKESLSFNPWRWQK----RNWRTSPFYCPFGGGTRFCPGAELARLQIALFLHYLIA 458
           DE Y+  +L F+PWRWQ     +    +P + PFGGG R CPG  LA++++ALFLH  + 
Sbjct: 194 DEKYHSSALKFDPWRWQPHLQDQELLKNPSFMPFGGGARLCPGMHLAKMELALFLHNFVT 373

Query: 459 CFRWTQLKEDRISFFPSARLVNGFKIQLNRRD 490
            FRW  L++D+IS+FP  RL+ G  I+L  R+
Sbjct: 374 KFRWEALQDDKISYFPFPRLIKGLXIRLRLRE 469

14  LNWDDYQSLKFTQCVIHETLRVGNFGPGVFRETKEDIKTKGGFVIPRGWTVYVFLTGTHL 193
194 DEKYHSSALKFDPWRWQPHLQDQELLKNPSFMPFGGGARLCPGMHLAKMELALFLHNFVT 373
374 KFRWEALQDDKISYFPFPRLIKGLXIRLRLRE 469


>BG318000.1 NXPV_008_D01_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_008_D01 5'.
          Length = 534
CYP720 like CYP85 clan
Query: 45  LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
           LPPGSTGWPLIGET S++R + S  +PRQFI +RE+RY  +IF  +LFG  + V+S D +
Sbjct: 137 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQEREQRY-GEIFRXNLFGRSRIVVSVDXE 313

Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFME 162
           FNK+VL +EGR F  +YP  L+ LI  YGLLSVHGDLQRKLHG  VNLL       DFME
Sbjct: 314 FNKHVLQHEGRQFXXNYPKPLRNLIGKYGLLSVHGDLQRKLHGAAVNLLXXXXXSVDFME 493

Query: 163 EI 164
           +I
Sbjct: 494 DI 499

>BI077317.1 NXPV_095_E11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus
          taeda cDNA clone NXPV_095_E11 5'.
          Length = 260
CYP90 like
Query: 1  MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
          MADQISLLLVVFTAAVALLHLIYRWWNAQR  KRT+N+ N E HLPPGSTG  L  ETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAQRXQKRTNNEKNPEGHLPPGSTGXXLXXETYS 194

Query: 61 YYRSMTSNRPRQFIDDREKRYD 82
          YYRSMT N P+QFI DREKRYD
Sbjct: 195YYRSMTXNXPKQFIHDREKRYD 260

>BE049731.1 NXNV_143_A08_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
           clone NXNV_143_A08 5'.
          Length = 456
CYP720 like cyp85 clan
Query: 45  LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
           LPPGSTGWPLIGET S++R + S  +PRQFI +RE+RY  +IF S+LFG  + V+S DP+
Sbjct: 126 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDPE 302

Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
           FNK+VL +  R FQ +YP  L+ LI  YGLLSVHGDL  KLHG  VNLL
Sbjct: 303 FNKHVLQHXXRQFQXNYPKPLRNLIGKYGLLSVHGDLXXKLHGAXVNLL 449

> BG040616.1 NXSI_113_B06_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_113_B06 5'.
          Length = 443
CYP720 like cyp85 clan
Query: 45  LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
           LPPGSTGWPLIGET S++R + S  +PRQFI +RE+RY  +IF S+LFG  + V+S D +
Sbjct: 109 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDXE 285

Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
           FNK+VL +E R FQ +YP  L+ LI  YGLLSVHGDL   LHG  VNLL
Sbjct: 286 FNKHVLQHEXRQFQXNYPKPLRNLIGKYGLLSVHGDLXXXLHGAAVNLL 432

> BE582456.1 NXCI_033_F03_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
           taeda cDNA clone NXCI_033_F03 5'.
          Length = 446

Query: 45  LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
           LPPGSTGWPLIGET S++R + S  +PRQFI +RE+RY  +IF S+LFG  + V+S DP+
Sbjct: 123 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDPE 299

Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
           FNK+VL +  R FQ +YP  L+ LI  YG LSVHGD   KLHG  VNLL
Sbjct: 300 FNKHVLQHXXRQFQXNYPKPLRNLIGKYGXLSVHGDXXXKLHGAXVNLL 446

MTETLTDSWLVGLLCLVLGFLLLQLYKLVWGASSRAYK
LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRYGEIFRSNLFGRSRIVVSVDPE
FNKHVLQHEGRQFQANYPKPLRNLIGKYGLLSVHGDLQKKLHGAAVNLL
Rferlsvdfmedi from BI416628.1 Scots pine

AW056514  Related but different seq 
81  MAETSVTYSWLVGFVCFVLATILLQLYRIVDSRGYNLPPGSRGWPLIGESLGFMREI 251
252 NSGSQPRRFIXDRELRYGEIFXSNLFGRSRLIVSVXXEFNKHVLQXXXRLFXANXPRPFR 431
432 N 434

AW011172 Related but different seq
52  MGETLHSLLVGLVCFALGMLLLELYKLVWRVDSRGYKLPPGSTGLPLIGETISFFRGIN 228
229 STDQPRRYIQEREKRYGEIFKSNLFGKISDCCVRGFKVQQTCPAARRQEFQANYPKPPPN 408
409 PNRKFWLLACTG 444

> AW011225.1 ST18B12 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST18B12.
          Length = 389
CYP90 like different from AA740068
Query: 218 GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMRLYNRE 275
           GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPM LYNRE
Sbjct: 3   GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMCLYNRE 176

>BF609170.1 NXSI_041_B05_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_041_B05 5'.
          Length = 504
CYP90 like
Query: 162 EEIDTKGXX-IPKGWVVFAFLLATHLDEXXXXXXXTFNPWRWEL---DQDVSNNHLFSPF 217
           E++ TKG   IPKGW V+ FL  THLDE       TFNPWRW+    DQ++S N  F PF
Sbjct: 4   EDVKTKGGFVIPKGWTVYVFLTGTHLDEKYHSSALTFNPWRWQQLLQDQELSKNPSFMPF 183

Query: 218 GGGARLCPGSHLARLELALFLHIFITRFR 246
           GGGARLCPG HLA+LELALFLH F+T+FR
Sbjct: 184 GGGARLCPGMHLAKLELALFLHNFVTKFR 270

>BI416628.1 hasp001xk19f Heterobasidion annosum - Scots pine infection stage
           subtraction cDNA library (hasp) Pinus
           sylvestris/Heterobasidion annosum cDNA clone
           hasp001xk19f.
          Length = 470
CYP85 clan
Query: 68  NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKAL 126
           +RPR +I +REKRY  +IF S+LFG  + V+S DP+FNK+VL +EGR FQA+YP  L+ L
Sbjct: 7   SRPR-YIQEREKRY-GEIFRSNLFGRSRIVVSVDPEFNKHVLQHEGRQFQANYPKPLRNL 180

Query: 127 IRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEEI 164
           I  +GLL ++GDLQ+KLHG  VNLL+FE+L  DFME+I
Sbjct: 181 IGKFGLLFLYGDLQKKLHGAAVNLLRFERLSVDFMEDI 294

>AW056514.1 ST51B01 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST51B01.
          Length = 435
CYP85 clan
Query: 44  HLPPGSTGWPLIGETYSYYRSMTS-NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDP 101
           +LPPGS GWPLIGE+  + R + S ++PR+FI DRE RY  +IF S+LFG  + ++S   
Sbjct: 186 NLPPGSRGWPLIGESLGFMREINSGSQPRRFIXDRELRY-GEIFXSNLFGRSRLIVSVXX 362

Query: 102 QFNKYVLLNEGRFFQAHYP 120
           +FNK+VL    R F A+ P
Sbjct: 363 EFNKHVLQXXXRLFXANXP 419

> BM428379.1 NXRV_015_D05_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
           clone NXRV_015_D05 5'.
          Length = 413
63% to 85A2
Query: 5   ISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRS 64
           I+L  +   + +ALL      WN  R  K+          LPPG+ GWPL GET  + R 
Sbjct: 35  IALGFIFLCSIIALLR-----WNEVRYGKKG---------LPPGTMGWPLFGETPDFLRY 172

Query: 65  MTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYPXALK 124
                 +QFI +R+ RY  D+F +H+ G   VIS+DP  N+Y+LLN  R        ++ 
Sbjct: 173 -----GQQFIKNRKARY-GDLFKTHILGCPTVISTDPALNRYILLNXXRGLIPGXXQSML 334

Query: 125 ALIRDYGLLSVHGDLQRKL 143
            ++    + +V  DL + +
Sbjct: 335 DILGKXXIAAVXXDLHKTM 391

> AW011172.1 ST17E09 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST17E09.
          Length = 519
56% to 87A2
Query: 9   LVVFTAAVALLHLIYRWWNAQ-RGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRSMTS 67
           LV F   + LL L    W    RG K           LPPGSTG PLIGET S++R + S
Sbjct: 85  LVCFALGMLLLELYKLVWRVDSRGYK-----------LPPGSTGLPLIGETISFFRGINS 231

Query: 68  -NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKA 125
            ++PR++I +REKRY  +IF S+LFG I        +  +       + FQA+YP     
Sbjct: 232 TDQPRRYIQEREKRY-GEIFKSNLFGKISDCCVRGFKVQQTCPAARRQEFQANYPKPPPN 408

Query: 126 LIRDYGLLS
             R + LL+
Sbjct: 409 PNRKFWLLA

Query: 135 VHGDLQRKLHGITVNLLKFEKL 156
           VHGD Q+KLHG  VN  K +KL
Sbjct: 435 VHGDFQKKLHGTXVNXFKVQKL 500

> BF778196.1 NXSI_080_C07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_080_C07 5'.
          Length = 250
87A2 like
Query: 45  LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLF 91
           LPPGSTGWPLIGET S++R +    +PRQFI +RE+ Y  +I   +LF
Sbjct: 109 LPPGSTGWPLIGETISFFRGINXTAQPRQFIQERERXY-GEIXXXNLF 249

> BF610049.1 NXSI_054_B07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_054_B07 5'.
          Length = 380
83% to 85A1
Query: 214 FSPFGGGARLCPGSHLARLELALFLHIFITRFR 246
           F+ FGGG+RLCPG  L  +++++FLH F+TR+R
Sbjct: 7   FTAFGGGSRLCPGKELGLVQISMFLHHFVTRYR 105

> BG625777.1 NXPV_066_A04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_066_A04 5'.
          Length = 453
50% to 90B1
Query: 259 KINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFA 318
           KI ++IE R Q+  A     N +L +LL+E+S   E +ADFI+ LLFAG+ET+S++M FA
Sbjct: 3   KIYESIEDRRQHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHETSSRSMSFA 173

Query: 319 VYFLTHCPKAMTQLQEEHD-------RLAGGMLTWQDYKTMDFTQCVIDETLRLGGIAIW 371
           + FLT CP+A+ +L+ EHD        L    L W DY+++ FTQCVI ETLR+G     
Sbjct: 174 IKFLTDCPRALEELKAEHDALLKRKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPG 353

Query: 372 LMREAKED
           + RE KED
Sbjct: 354 VFRETKED



> BG832634.1 NXPV_077_F04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_077_F04 5'.
          Length = 436

Query: 259 KINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFA 318
           KI ++IE R Q+  A     N +L +LL+E+S   E +ADFI+ LLFAG+ET+S++M FA
Sbjct: 3   KIYESIEDRRQHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHETSSRSMSFA 173

Query: 319 VYFLTHCPKAMTQLQEEHD-------RLAGGMLTWQDYKTMDFTQCVIDETLRLGGIAIW 371
           + FLT CP+A+ +L+ EHD        L    L W DY+++ FTQCVI ETLR+G     
Sbjct: 174 IKFLTDCPRALEELKAEHDALLKXKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPX 353

Query: 372 LMREAK
           + RE K
Sbjct: 354 VFRETK

> AW225716.1 ST71A02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
           ST71A02.
          Length = 234

Query: 251 QARKEIIRKINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNET 310
           +AR+ +I+KI ++IE R  +  A     N +L +LL+E+S   E +ADFI+ LLFAG+ET
Sbjct: 7   KAREFLIKKIYESIEDRXXHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHET 177

Query: 311 TSKTMLFAVYFLTHCPKAM 329
           +S+ M FA+  LT CP+A+
Sbjct: 178 SSRXMSFAIKXLTDCPRAL 234

> BG039994.1 NXSI_104_E01_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_104_E01 5'.
          Length = 553
74% to 98A3
Query: 285 LLEEESLPNESMADFIINLLFAGNETTSKTMLFAVYFLTHCPKAMTQLQEEHDRLAGG-- 342
           L E+  L  +++   + +++ AG +TT+ T+ +A+  L   P+   + QEE DR+ G   
Sbjct: 111 LQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEIDRVVGRDR 290

Query: 343 MLTWQDYKTMDFTQCVIDETLRL-GGIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVH 401
           ++   D+  + + QC+  E LRL     + L  +A ++V    Y IPKG  V   + A+ 
Sbjct: 291 VMNETDFPHLPYLQCITKEXLRLHPPTPLMLPHKATQNVKXXGYDIPKGSNVHXXVWAIA 470

Query: 402 LDESYYKE 409
            D + +K+
Sbjct: 471 RDPAVWKD 494


> AI725152.1 1051 PtIFG2 Pinus taeda cDNA clone 9047r.
          Length = 395
Like CYP720
Query: 281 VLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFAVYFLTHCPKAMTQLQEEHDRL 339
           +L +LL ++S+ +E   DFI+ LLFAG+ T+SK    A  FLT CP+    L + H+R+
Sbjct: 84  LLTKLLXQDSISDEITCDFILFLLFAGHXTSSKXXTXATKFLTTCPR----LIDSHERI 248

> BM158852.1 NXLV_040_D06_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
           clone NXLV_040_D06 5'.
          Length = 401
57% to 98A3
Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRWT 463
           PFG GT+ CPG +LA L +   L  L+ CF WT
Sbjct: 23  PFGAGTKVCPGEQLAMLLVQFPLAXLLHCFYWT 121

> AW870094.1 NXNV_124_A07_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
           clone NXNV_124_A07 5'.
          Length = 375
60% to 76C2
Query: 326 PKAMTQLQEEHDRLAGG--MLTWQDYKTMDFTQCVIDETLRLGGIA-IWLMREAKEDVSY 382
           P  + + Q E D + G    +   D   + + Q V+ E LRL   A + + R A      
Sbjct: 8   PTLLKKAQAELDEVVGREKRMEESDIAKLPYLQAVVKEVLRLHPAAPLIIPRRADNSAEI 187

Query: 383 QDYVIPKGCFVVPFLSAVHLDESYYKESLSFNPWRWQ--KRNWRTSPF-YCPFGGGTRFC 439
             YV+P+   V   +  +  D + +KE L F P R+   K ++R   F   PFG     C
Sbjct: 188 GGYVVPENTQVFVNIWGIVRDPNVWKEPLKFKPERFLXCKTDYRXXDFELIPFGAXXXIC 367

>AW290673.1 NXNV044H02F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
           NXNV044H02 5'.
          Length = 374
81% to 51A2
Query: 343 CIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDT 402
           CIKEALRLHPPLI+L+R++H DF+VTA+DGK Y IPKGH+VATSPAFANRLPHIFK+PDT
Sbjct: 1   CIKEALRLHPPLIVLLRSNHRDFTVTAKDGKDYVIPKGHVVATSPAFANRLPHIFKNPDT 180

Query: 403 YDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPF 462
           YDP+RF PGREEDK  GAFSYI+FGGGRHGCLGEPFAYLQIKAIW+HLLRNFE  L+SP 
Sbjct: 181 YDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEXXLISPX 360

Query: 463 PEID 466
            E D
Sbjct: 361 XEXD 372
CYP51 N-term
MDFIQNIYVQAGLLILATLVLAKIVSSILGFGSSKNLPPMVPAWPIVGGLMKFLKGPIVMLR
EEYPKLGNVFTIKLLIKNVTFLIGPEVSQHFFKXPESDL
CYP51 C-term
LXHDVLAXXDVLYRCIKEALRLHPPLIVLLRSNHRDFTVTAKDGKDYVIPKGHVVATSPAFANRLPHIFKNPDT
YDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFELELISP
FPEIDWNAMVVGVKDKVMVRYRRRPLSVD*

>CYP51A2 Arabidopsis missing part
SQQEVYQFNVPTFGPG
VVFDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLI
ILTASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEI
FAKIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWT
GAYLMRYKEYFSAALDEQKNLIAKHGDK

>gi|11604806|gb|BF517195.1|BF517195 NXSI_011_E08_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_011_E08 5'.
          Length = 538

 Score =  212 bits (540), Expect = 9e-56
 Identities = 97/110 (88%), Positives = 104/110 (94%)
 Frame = +3

Query: 379 KGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPF 438
           KGH+VATSPAFANRLPHIFK+PDTYDP+RF PGREEDK  GAFSYI+FGGGRHGCLGEPF
Sbjct: 3   KGHVVATSPAFANRLPHIFKNPDTYDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPF 182

Query: 439 AYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRYKRRQLS 488
           AYLQIKAIW+HLLRNFELEL+SPFPEIDWNAMVVGVK  VMVRY+RR LS
Sbjct: 183 AYLQIKAIWTHLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYRRRPLS 332

>gi|8179218|gb|AW984874.1|AW984874 NXNV_119_G10_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
           clone NXNV_119_G10 5'.
          Length = 292

 Score =  186 bits (471), Expect = 9e-48
 Identities = 84/96 (87%), Positives = 89/96 (92%)
 Frame = +3

Query: 387 PAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPFAYLQIKAI 446
           PAFANRLPHIFK+PDTYDP+RF PGREEDK  GAFSYI+FGGGRHGCLGEPFAYLQIKAI
Sbjct: 3   PAFANRLPHIFKNPDTYDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAI 182

Query: 447 WSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482
           W+HLLRNFELEL+SPFPEIDWNAMVVGVK   M RY
Sbjct: 183 WTHLLRNFELELISPFPEIDWNAMVVGVKDXXMXRY 290

>gi|7363541|gb|AW626637.1|AW626637 NXNV067F11 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
           NXNV067F11 5'.
          Length = 257

 Score =  145 bits (366), Expect = 1e-35
 Identities = 64/85 (75%), Positives = 76/85 (89%)
 Frame = +2

Query: 329 IDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHIVATSPA 388
           + HD+L+  DVLYRCIKEALRLHPPLI+L+R++H DF+VTA+DGK Y IPKGH+VATSP 
Sbjct: 2   LXHDVLAXXDVLYRCIKEALRLHPPLIVLLRSNHKDFTVTAKDGKDYVIPKGHVVATSPX 181

Query: 389 FANRLPHIFKDPDTYDPERFSPGRE 413
           FANRLPHIF +PDTYDP+RF PGRE
Sbjct: 182 FANRLPHIFXNPDTYDPDRFVPGRE 256

>gi|11778796|gb|BF610290.1|BF610290 NXSI_057_D10_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
           clone NXSI_057_D10 5'.
          Length = 351

 Score =  120 bits (302), Expect = 3e-28
 Identities = 61/99 (61%), Positives = 77/99 (77%)
 Frame = +2

Query: 6   ENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPIIMLR 65
           +N  ++ GL+I+ATLV+AK++ S     S K  LPP + AWP +VG L+KFLKGPI+MLR
Sbjct: 59  QNIYVQAGLLILATLVLAKIVSSILGFGSSKN-LPPMVPAWP-IVGGLMKFLKGPIVMLR 232

Query: 66  EEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDL 104
           EEYPKLG+VFT+ L+ K +TFLIGPEVS HFFK  ESDL
Sbjct: 233 EEYPKLGNVFTIKLLIKNVTFLIGPEVSQHFFKXPESDL 349

> AW888136.1 NXNV_129_F04_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
           clone NXNV_129_F04 5'.
          Length = 242
85% to 98A3
Query: 403 DESYYKESLSFNPWRWQKRNWRTSPF---YCPFGGGTRFCPGAELARLQIALFLHYLIAC 459
           D + +K+ ++F P R+ + +           PFG G R CPGA+L    +   L +L+  
Sbjct: 4   DPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGINLVQSMLGHLLHH 183

Query: 460 FRW 462
           F W
Sbjct: 184 FVW 192


> BE187484.1 NXNV_98_G10_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
           NXNV_98_G10 5'.
          Length = 614
87% to 73A5
Query: 352 MDFTQCVIDETLRLG-GIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHLDESYYKES 410
           + + Q V+ ETLRL   I + +      D     Y IP    ++     +  + + +K  
Sbjct: 21  LPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNP 200

Query: 411 LSFNPWRWQKRNWRTSPF-----YCPFGGGTRFCPGAELARLQIALFLHYLIACF 460
             F P R+ +    T        + PFG G R CPG  LA   +AL +  L+  F
Sbjct: 201 EEFRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPLLALSIGRLVQNF 365

> BM158467.1 NXLV_034_H02_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
           clone NXLV_034_H02 5'.
          Length = 585
68% to 78A5
Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRW 462
           PFG G R CPG  L    + L++  L+  F+W
Sbjct: 41  PFGAGRRVCPGKALGLATVNLWVAKLLHHFQW 136

> BG625848.1 NXPV_066_G08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
           cDNA clone NXPV_066_G08 5'.
          Length = 251
71% to 98A3
Query: 337 DRLAGG--MLTWQDYKTMDFTQCVIDETLRLGG-IAIWLMREAKEDVSYQDYVIPKGCFV 393
           DR+ G   ++   D+  + + QC+  E LRL     + L  +A ++V    Y IPKG  V
Sbjct: 5   DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGXNV 184

Query: 394 VPFLSAVHLDESYYKESLSFNP 415
              + A+    + +K   +F P
Sbjct: 185 HVNVWAIAR XXAVWKXPXTFKP 250


> AW754540.1 PC03D04 Pine TriplEx pollen cone library Pinus taeda cDNA clone
           PC03D04.
          Length = 464

Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRW 462
           PF  G R CPGA L    + L L  L   F W
Sbjct: 59  PFSAGKRKCPGAPLGTCFVLLALARLFHSFHW 154

> AW290024.1 NXNV009G04F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
           NXNV009G04 5'.
          Length = 438
84% to 73A5
Query: 354 FTQCVIDETLRLG-GIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHLDESYYKESLS 412
           + Q V+ ET  L   I + +      D     Y IP    ++     +  + + +K    
Sbjct: 2   YLQAVVKETXXLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEE 181

Query: 413 FNPWRWQKRNWRTSPF-----YCPFGGGTRFCPGAELARLQIALFLHYLIACF 460
           F P R+ +    T        + PFG G R CPG  LA   +AL +  L+  F
Sbjct: 182 FRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPLLALSIGRLVXNF 340