Revised conifer P450s New Jan. 6, 2004
311 conifer P450 accession numbers New Jan. 6, 2004
These are the old Pinus sequences. Please see the links above for
New data, including the sequences below.
74 P450 ESTs from Pinus
last modified Oct. 11, 1999 more ESTs added 2/19/02 (not exhaustive searches)
4 full length sequences are now available from mRNAs or assembled from ESTs
CYP73A20 pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA
CYP73A23 sequence assembled from ESTs AI812813, AA556941, AI812771, AI812842,
AI919827, AW495794.1 BE762259 BF517288 BE762275
MGSEVLAQGSALLEKSLIALLVVVVGAVLVNKLRGRKLKLPPGPFALPIFGNWLQVGDDLNHRNLSDLAK
KYGKIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKM
RRIMTVPFFTNKVVQHYRFAWEDEISRV
VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA
QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK
CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT
TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK
NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL
VQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA
CYP78A4 AF049067 Pinus radiata (Monterey pine)
MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPL
YIALLIVAVCAVLVSWLSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLPHRK
LESLARLHGAKQLMSFSLGCTPAVITSDPEVARELLTSPHFANRPLKQSAQQLLFGRA
IGFAPNGGYWRLLRRIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVR
RHLQGAALNNIMGSVFGRRFDMSHENEEVKKLREMVDEGFQLLGAFNWADHLPWLRPL
DPLRIHARCARLVPRVTTFVSNIIEQHRREEQRRESGDQCDFVDVLLSLQGEDKLDEE
DMIAVLWEMIFRGTDTTALLTEWTMAELVLHPEAQKKAQAELDAVVGHDRSVKDSDIP
KLPYIQAVVKEALRMHPPGPLLSWARLSTEDVNMGDGMCVPAGTTAMVNMWSITHDPN
IWESPYEFRPERFVVFEGGEEVDVRGNDLRLAPFGAGRRVCPGKALGLATVNLWVAKL
LHHFEWLPHAEHPVDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD
CYP98A15 AY064170 Pinus taeda p-coumarate 3-hydroxylase
MSVPEMGPFGVVVTGIVAIAIVYKLVQRWRFKLPPGPRPWPVVG
NLLQIEPVRFRCFWDWSKKYGPIMSVWFGSTLNVVVSNTELAKEVLKEHDQQLADRPR
SRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVAAMVESI
FNDCGKQEGIGKPLVVKRYLSGVAFNNITRPAFGKRFVNEEGKMDPQGVEFKEIVATG
LKLGASLTMAEHIPYLRWMFPLEEGAFAKHGARRDNVTKAIMEEHTLARQTSGAKQHF
VDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEI
DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGS
NVHVNVWAIARDPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGI
NLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRLPDHLYKR
QPLN
BF610290.1 CYP51 N-term
BF517195.1 CYP51 C-term
AW984874.1 CYP51 C-term
AW626637.1 CYP51 C-term
AW290673.1 84% to 51A2 C-term
AW043075 71D like 55% to 71D16
AI919813 84 like 53% identical to CYP84
AW010433 84 like 51% to CYP84
AI812674 73A20
AI920132 73A20
AI812813 73A23
AA556941 73A23
AI812771 73A23
AI812842 73A23
AI919827 73A23
AW042602 73A23
BF610527.1 73A23
BF609655.1 73A23
BG275633.1 73A23
BE187484.1 73A23
BE643861.1 73A23
AW290024.1 73A23
AW495794.1 73A23
AA220912 extreme C-term like 73A20 might be accidental match
AW056850 75A like 65% to 75A3
AW043335 75A like 55% identical to CYP75A3 53% identical to CYP98A1
AW870094.1 60% to 76C2
AW043205 77A 61% to 77A1
AI812861 77A 53% to 77A1 N-term
AA220898 78A4
AA220888 78A4
AA220889 78A4
AA220911 78A4
AI813156 78A 55% to 78A6
BM158467.1 68% to 78A5
AA556173 98A 80% to 98A2
BG039994.1 74% to 98A3
AW888136.1 85% to 98A3
BG625848.1 71% to 98A3
BM158852.1 57% to 98A3
BE187393.1 98A C-term
BG040095.1 98A N-term
BG039083.1 98A mid
AI920165 704 like 58% to 704A2 86 clan
AI813004 704 like, same seq as AI920165 86 clan
AW010774 52% to 86C1 in 86 clan
AA740068 90 like C-term 50% to 90A1 85 clan clone 9312M
AI725255 90 like C-term clone 9159r
AA739879 90 like C-term clone 9047M
AI725351 90 like N-term 40% to 90A1 clone 9312r opposite end of AA740068
all four of these 90 like ESTs are from the same gene
have the first 164 amino acids and the C-terminal. Missing about 208 aa
in the middle.
BG318892.1 CYP90 like different from AA740068 *
BG318205.1 CYP90 like different from AA740068 *
BI202885.1 CYP90 like different from AA740068 *
BG318334.1 CYP90 like different from AA740068 *
BI644007.1 CYP90 like *
BI077317.1 CYP90 like *
AW011225.1 CYP90 like different from AA740068
BI398025.1 CYP90 like
BF609170.1 CYP90 like
BG318000.1 CYP85 clan
BE049731.1 CYP85 clan
BG040616.1 CYP85 clan
BE582456.1 CYP85 clan
BI416628.1 CYP85 clan
AW056514.1 CYP85 clan
BF610049.1 83% to 85A1
BM428379.1 63% to 85A2
AW011172.1 56% to 87A2
BF778196.1 87A2 like
BG625777.1 50% to 90B1
BG832634.1 50% to 90B1
AW225716.1 50% to 90B1
AI725152.1 CYP720 like
AW754540.1 81% to 703A2
many of these sequences are from The Pine Gene Discovery Project
Whetten,R.W., Kinlaw,C.S., Retzel,E., Sederoff,R.R.
Allona I, Quinn M, Shoop E, Swope K, Cyr SS, Carlis J, Riedl J, Retzel E, Campbell MM,
Sederoff R, Whetten RW (1998)
Analysis of xylem formation in pine by cDNA sequencing.
Proc Natl Acad Sci U S A 95, 9693-9698.
go to abstract
CYP98A AY064170 Pinus taeda p-coumarate 3-hydroxylase
MSVPEMGPFGVVVTGIVAIAIVYKLVQRWRFKLPPGPRPWPVVG
NLLQIEPVRFRCFWDWSKKYGPIMSVWFGSTLNVVVSNTELAKEVLKEHDQQLADRPR
SRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLELFSPKRLEALRPIREDEVAAMVESI
FNDCGKQEGIGKPLVVKRYLSGVAFNNITRPAFGKRFVNEEGKMDPQGVEFKEIVATG
LKLGASLTMAEHIPYLRWMFPLEEGAFAKHGARRDNVTKAIMEEHTLARQTSGAKQHF
VDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEI
DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGS
NVHVNVWAIARDPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGI
NLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRLPDHLYKR
QPLN
>gi|12482680|gb|BG040095.1|BG040095 NXSI_106_D11_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_106_D11 5'.
Length = 480
Score = 230 bits (587), Expect = 3e-61
Identities = 116/160 (72%), Positives = 130/160 (80%)
Frame = +1
98A N-term
Query: 70 LNVVVSSAELAKEVLKEHDQKLADRHRNRSTEAFSRNGQDLIWADYGPHYVKVRKVCTLE 129
LNVVVS+ ELAKEVLKEHDQ+LADR R+RS E FSRNGQDLIWADYGPHYVKVRKVCTLE
Sbjct: 1 LNVVVSNTELAKEVLKEHDQQLADRPRSRSAEKFSRNGQDLIWADYGPHYVKVRKVCTLE 180
Query: 130 LFTPKRLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGK 189
LF+PKRLE+LRPIREDEV AMVES+F DC E K L +++YL VAFNNITRLAFGK
Sbjct: 181 LFSPKRLEALRPIREDEVAAMVESIFNDCGKQEGIGKPLVVKRYLSGVAFNNITRLAFGK 360
Query: 190 RFMNAEGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLR 229
RF+N G +D +EF IV+ GLKLGAS AEH +LR
Sbjct: 361 RFVNEXGKMDPXXVEFXXIVATGLKLGASPHXAEHXXYLR 480
>gi|12481668|gb|BG039083.1|BG039083 NXSI_092_E06_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_092_E06 5'.
Length = 491
98A like mid region
Query: 135 RLESLRPIREDEVTAMVESVFRDCNLPENRAKGLQLRKYLGAVAFNNITRLAFGKRFMNA 194
RLE+LRPIREDEV AMVES+F DC E K L +++YL VAFNNITRLAFGKRF+N
Sbjct: 1 RLEALRPIREDEVAAMVESIFNDCGKQEGIGKPLVVKRYLSGVAFNNITRLAFGKRFVNE 180
Query: 195 EGVVDEQGLEFKAIVSNGLKLGASLSIAEHIPWLRWMFPADEKAFAEHGARRDRLTRAIM 254
EG + QG+EFK IV+ GLKLGASL++AEHIP+LRWMFP E AFA+HGARRD +T+AIM
Sbjct: 181 EGKMXPQGVEFKXIVATGLKLGASLTMAEHIPYLRWMFPLXEGAFAKHGARRDNVTKAIM 360
Query: 255 EEHTLARQKSSGAKQHFVDALLTLQEKYDLSEDTIIGLLWDM 296
EHTLARQ + GAKQHFVDALLTLQEKYD SE TI LWDM
Sbjct: 361 XEHTLARQ-TXGAKQHFVDALLTLQEKYDXSEXTIXXXLWDM 483
>gi|8666577|gb|BE187393.1|BE187393 NXNV_163_D07_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_163_D07 5'.
Length = 591
CYP98A
Query: 437 ICPGAQLGINLVQSMLGHLLHHFVWTPPQGTKPEEIDMSENPGLVTYMRTPVQAVATPRL 496
ICPGAQLGINLVQSMLGHLLHHFVW PP+G + E+ID++ENPGLVT+M PVQA+A PRL
Sbjct: 1 ICPGAQLGINLVQSMLGHLLHHFVWAPPEGMQAEDIDLTENPGLVTFMAKPVQAIAIPRL 180
Query: 497 PSDLYKRVP 505
P LYKR P
Sbjct: 181 PDHLYKRQP 207
73A20 search pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA
AI812674 17G2 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
2 diffs with 73A20
Query: 1 MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN 60
MEIMTVASLEKGLLAIFAVIVGAIFIS LKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN
Sbjct: 47 MEIMTVASLEKGLLAIFAVIVGAIFISNLKSKKLKLPPGPLAVPIFGNWLQVGDDLNHRN 226
Query: 61 LGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM 120
LGDLA KYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM
Sbjct: 227 LGDLANKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDM 406
Query: 121 VFTVYGEHWR 130
VFTVYGEHWR
Sbjct: 407 VFTVYGEHWR 436
gb|AI920132.1|AI920132 1662 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
f1_PL2CSUH, mRNA sequence
Length = 615
Score = 41.4 bits (95), Expect = 9e-05
Identities = 23/29 (79%), Positives = 24/29 (82%)
Frame = +2
73A20
Query: 478 QSKVDVTEKGGQFSLHILNHSVVVAKPIA 506
QSKV E+ QFSLHILNHSVVVAKPIA
Sbjct: 143 QSKVMCRERR-QFSLHILNHSVVVAKPIA 226
gb|AI812813.1|AI812813 19E5 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 489
Score = 277 bits (701), Expect = 8e-76
Identities = 133/162 (82%), Positives = 149/162 (91%), Gaps = 1/162 (0%)
Frame = +1
Query: 161 VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSRLA 220
V D+K R E+ST+GIVIR+RLQL+MYNIMYRMMFDRRFESEDDPLFL+LKALNGERSRLA
Sbjct: 1 VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA 180
Query: 221 QSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVTGD-K 279
QSFEYNYGDFIP+LRPFLRGYL+IC E+KE RL LFKDYF++ERKKL ST S TG+ K
Sbjct: 181 QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK 360
Query: 280 CAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGL 322
CA+DHI +A+ KGEINEDNVLYIVENINVAAIETTLWSMEWG+
Sbjct: 361 CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGI 489
73A23 sequence assembled from ESTs AI812813, AA556941, AI812771, AI812842,
AI919827, AW495794.1 BE762259 BF517288 BE762275
MGSEVLAQGSALLEKSLIALLVVVVGAVLVNKLRGRKLKLPPGPFALPIFGNWLQVGDDLNHRNLSDLAK
KYGKIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGDHWRKM
RRIMTVPFFTNKVVQHYRFAWEDEISRV
VADVKSRAESSTSGIVIRKRLQLMMYNIMYRMMFDRRFESEDDPLFLKLKALNGERSRLA
QSFEYNYGDFIPILRPFLRGYLRICNEIKEKRLSLFKDYFVEERKKLNSTKTSTNTGELK
CAMDHILDAQNKGEINEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT
TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK
NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL
VQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA
73A20 search pinus taeda from AF096998
MEIMTVASLEKGLLAIFAVIVGAIFISKLKSKKLKLPPGPLAVP
IFGNWLQVGDDLNHRNLGDLAKKYGEIFLLKMGQRNLVVVSSPDLAKEVLHTQGVEFG
SRTRNVVFDIFTGKGQDMVFTVYGEHWRKM
RRIMTVPFFTNKVVQQYRFAWEDELGRA
VDDIKKRPEASTTGIVIRRRLQLVMYNIMYRMMFDRRFESEDDPLFLRLKALNGERSR
LAQSFEYNYGDFIPVLRPFLRGYLKICKEVKESRLLLFKDYFIDERKKLASTTGSKVT
GDKCAIDHIFEAEEKGEINEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQK
IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYD
IPAESKILVNAWFLANNPEWWEKPEEFIPERFLGEEKIEASGNDFRFLPFGVGRRSCP
GIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA
gb|AA556941|AA556941 783 Loblolly pine C Pinus taeda cDNA clone 7C7B
Length = 678
Score = 263 bits (666), Expect(2) = 2e-75
Identities = 127/154 (82%), Positives = 139/154 (89%), Gaps = 1/154 (0%)
Frame = +2
Query: 295 NEDNVLYIVENINVAAIETTLWSMEWGLAEIVNHPEIQQKIRAELDAVIGRGVPLTEPDT 354
NEDNVLYIVENINVAAIETTLWSMEWG+AE+VNH +IQ K+RAELDAV+G GV TEPDT
Sbjct: 2 NEDNVLYIVENINVAAIETTLWSMEWGIAELVNHQDIQSKVRAELDAVLGPGVQXTEPDT 181
Query: 355 TKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKILVNAWFLANNPEWWE 414
T+LPYLQAVVKETLRL MAIPLLVPH NLH AKLGGYDIPAESKILVNAW+LANNP W+
Sbjct: 182 TRLPYLQAVVKETLRLRMAIPLLVPHXNLHDAKLGGYDIPAESKILVNAWWLANNPANWK 361
Query: 415 KPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPGIILALPILALALGRL 466
PEEF PERF EEK EA+GNDF+FLPFGVGR++CPGIILALP+LAL++GRL
Sbjct: 362 NPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRKTCPGIILALPLLALSIGRL 515
Query: 467 VQNFELLPPPGQSKVDVTEKGGQFSLHILN 496
V F LLPPPGQ+K + EKGGQ L ILN
Sbjct: 516 VXXFHLLPPPGQTKW-IPEKGGQSPLXILN 603
gb|AI812771.1|AI812771 18H4 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 518
Score = 295 bits (747), Expect = 3e-81
Identities = 143/167 (85%), Positives = 155/167 (92%), Gaps = 1/167 (0%)
Frame = +2
Query: 340 DAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIPAESKI 399
DAV+G GV +TEPDTT+LPYLQAVVKETLRL MAIPLLVPHMNLH AKLGGYDIPAESKI
Sbjct: 2 DAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKI 181
Query: 400 LVNAWFLANNPEWWEKPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPGIILALPI 458
LVNAW+LANNP W+ PEEF PERF EEK EA+GNDF+FLPFGVGRRSCPGIILALP+
Sbjct: 182 LVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPL 361
Query: 459 LALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVVVAKPIA 506
LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNHS++VAKPIA
Sbjct: 362 LALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLIVAKPIA 505
gb|AI812842.1|AI812842 20F6 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 539
Score = 192 bits (484), Expect = 2e-50
Identities = 93/117 (79%), Positives = 101/117 (85%), Gaps = 1/117 (0%)
Frame = +2
Query: 335 IRAELDAVIGRGVPLTEPDTTKLPYLQAVVKETLRLHMAIPLLVPHMNLHQAKLGGYDIP 394
+RAELDAV+G GV +TEPDTT+LPYLQAVVKETLRL MAIPLLVPHMNLH AKLGGYDIP
Sbjct: 2 VRAELDAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIP 181
Query: 395 AESKILVNAWFLANNPEWWEKPEEFIPERFLGEEK-IEASGNDFRFLPFGVGRRSCPG 451
AESKILVNAW+LANNP W+ PEEF PERF EEK EA+GNDF+FLPFG G PG
Sbjct: 182 AESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDFKFLPFGCGEEELPG 355
Score = 122 bits (303), Expect = 4e-29
Identities = 58/66 (87%), Positives = 63/66 (94%)
Frame = +3
Query: 441 PFGVGRRSCPGIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNHSVV 500
P VGRRSCPGIILALP+LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNHS++
Sbjct: 324 PSVVGRRSCPGIILALPLLALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNHSLI 503
Query: 501 VAKPIA 506
VAKPIA
Sbjct: 504 VAKPIA 521
gb|AI919827.1|AI919827 1371 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
C7_PL12CSUH, mRNA sequence
Length = 807
Score = 129 bits (321), Expect = 3e-31
Identities = 61/69 (88%), Positives = 67/69 (96%)
Frame = +1
Query: 438 RFLPFGVGRRSCPGIILALPILALALGRLVQNFELLPPPGQSKVDVTEKGGQFSLHILNH 497
+FLPFGVGRRS PGIILALP+LAL++GRLVQNF LLPPPGQSKVDVTEKGGQFSLHILNH
Sbjct: 370 KFLPFGVGRRSXPGIILALPLLALSIGRLVQNFHLLPPPGQSKVDVTEKGGQFSLHILNH 549
Query: 498 SVVVAKPIA 506
S++VAKPIA
Sbjct: 550 SLIVAKPIA 576
CYP78A4 search pinus radiata
AA220898 13 Pinus radiata somatic embryogenic library Pinus radiata cDNA
4 differences to 78A4
Query: 175 RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF 234
RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF
Sbjct: 3 RIASAHLFAPRRIAAHEAGRQADVVAMLDDIQKEYHSKGVVRVRRHLQGAALNNIMGSVF 182
Query: 235 GRRFDMSHENEEVKKLREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVPRVT 293
GRRFDMSHENEEVK+LREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVP VT
Sbjct: 183 GRRFDMSHENEEVKRLREMVDEGFQLLGAFNWADHLPWLRPLDPLRIHARCARLVPSVT 359
FVSNIIEQHRREEQRR 310
FV NIIE+HRREEQRR
FVRNIIEEHRREEQRR 409
AA220888 3 Pinus radiata somatic embryogenic library Pinus radiata cDNA
3 diffs to 78A4
Query: 1 MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW 60
MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW
Sbjct: 73 MENRRSSGGSGWWVCVLPLFTKDGPAYFLHSSSDDVSAWRQWPLYIALLIVAVCAVLVSW 252
Query: 61 LSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLP 99
LSPGGCAWAGRHK+ RVAIPGPKGWPIIG LMDMSVGLP
Sbjct: 253 LSPGGCAWAGRHKKSRVAIPGPKGWPIIGILMDMSVGLP 369
AA220911 26 Pinus radiata somatic embryogenic library Pinus radiata cDNA
1 diff to 78A4
Query: 522 VDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD 553
VDLSEVLKLSCEMARPLHCVP TRVPFAKFSD
Sbjct: 14 VDLSEVLKLSCEMARPLHCVPGTRVPFAKFSD 109
gb|AA220889|AA220889 4 Pinus radiata somatic embryogenic library Pinus radiata cDNA
clone pPRE74D 3'
Length = 394
Score = 33.6 bits (75), Expect = 0.022
Identities = 16/23 (69%), Positives = 17/23 (73%)
Frame = +3
Query: 514 WLPHAEHPVDLSEVLKLSCEMAR 536
W HAEHPV + LKLSCEMAR
Sbjct: 3 WFQHAEHPVIVGG-LKLSCEMAR 68
CYP98A2 search
gb|AA556173|AA556173 28 Loblolly pine N Pinus taeda cDNA clone 2N4B
Length = 710
Score = 260 bits (657), Expect(2) = 3e-77
Identities = 122/153 (79%), Positives = 138/153 (89%)
Frame = +3
CYP98A subfamily
Query: 247 RDRLTRAIMAEHTEARKKSGGAKQHFVDALLTLQDKYDLSEDTIIGLLWDMITAGMDTTA 306
RD +T+AIM EHT AR+ SG AKQHFVDALLTLQ+KYDLSEDTIIGLLWDMITAGMDTTA
Sbjct: 15 RDNVTKAIMEEHTLARQTSG-AKQHFVDALLTLQEKYDLSEDTIIGLLWDMITAGMDTTA 191
Query: 307 ISVEWAMAELIRNPRVQQKVQEELDRVIGLERVMTEADFSNLPYLQCVTKEAMRLHPPTP 366
I+VEWAMAEL+RNPR+ QK QEE+ RV+G +RVM E DF +LPYLQC+TKEA+RLHPPTP
Sbjct: 192 ITVEWAMAELVRNPRIXQKAQEEIXRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTP 371
Query: 367 LMLPHRANANVKVGGYDIPKGSNVHVNVWAVARDPAVWKDP
LMLPH+A NVK+GGYDIP GSNVHVNVWA+ARDPAVWK P
Sbjct: 372 LMLPHKATQNVKIGGYDIPXGSNVHVNVWAIARDPAVWKGP
gb|AI920165.1|AI920165 1695 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
h2_PL3CSUH, mRNA sequence
Length = 742
like CYP86A8
Score = 84.3 bits (205), Expect = 1e-17
Identities = 50/98 (51%), Positives = 61/98 (62%), Gaps = 3/98 (3%)
Frame = +2
58% to 704A2
Query: 413 GRMKSTWGEDCLEFKPERWISQSDGGRFINH--DPFKFVAFNAGPRICLGKDLAYLQMKS 470
GRM W +D EFKPERWI DG I+H PFKF+AF AGPR CLGKD AYLQMK
Sbjct: 245 GRMSYXWWDDAKEFKPERWIQ--DG---ISHPKSPFKFLAFQAGPRTCLGKDFAYLQMKI 409
Query: 471 IASAVLLRHRLTVVTGHKVEQKMSLTLFM-KYGLLVNVHER 510
+A+ ++ + V +V + LTL M + GL V V R
Sbjct: 410 VAAVLVRFFKFEAVKTKEVRYRTMLTLHMNEDGLNVQVTPR 532
gb|AI813004.1|AI813004 2F2 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 594
Score = 58.2 bits (138), Expect = 9e-10
Identities = 34/73 (46%), Positives = 42/73 (56%), Gaps = 1/73 (1%)
Frame = +1
Query: 438 GRFINHDPFKFVAFNAGPRICLGKDLAYLQMKSIASAVLLRHRLTVVTGHKVEQKMSLTL 497
G F PFK AF AGPR CLGKD AYLQMK +A+ ++ + V +V + LTL
Sbjct: 10 GIFHPKSPFKXPAFQAGPRTCLGKDFAYLQMKIVAAVLVRXFKFEAVKTKEVRYRTMLTL 189
Query: 498 FM-KYGLLVNVHER 510
M + GL V V R
Sbjct: 190 HMNEDGLNVQVTPR 231
gb|AA740068|AA740068 833 PtIFG2 Pinus taeda cDNA clone 9312M 3'
Length = 572
Score = 36.0 bits (81), Expect = 0.004
Identities = 23/79 (29%), Positives = 36/79 (45%), Gaps = 1/79 (1%)
Frame = +3
Query: 443 WSITHDPNIWESPYEFRPERFVVFEGGEEVDVRGNDLRLAPFGAGRRVCPGKALGLATVN 502
W +P+ W+S + + + + DV N L +PFG G R+CPG L +
Sbjct: 3 WXFDCNPSRWKSS-TMKXLHLIPWRWELDQDVSNNHL-FSPFGGGARLCPGSHLARLELA 176
Query: 503 LWVAKLLHHFEWLPHA-EHP 521
++ + F W A EHP
Sbjct: 177 XFLHIFITRFRWEALADEHP 236
DTKGXXIPKGWVVFAFLLATHLDEXXXXXXXTFNPWRWELDQDVSNNHLFSPFGGGARLCPGSHLARLELA 176
LFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMRLYNRE* 236
gb|AA739879|AA739879 644 PtIFG2 Pinus taeda cDNA clone 9047M 3'
Length = 604
Score = 30.1 bits (66), Expect = 0.22
Identities = 26/74 (35%), Positives = 34/74 (45%), Gaps = 4/74 (5%)
Frame = +2
Query: 397 SKILVNAWFLANNPEWWEKPE----EFIPERFLGEEKIEASGNDFRFLPFGVGRRSCPGI 452
SK + F A NP + P IP R+ ++ + N+ F PFG G R CPG
Sbjct: 38 SKRMGGVCFFACNPSR*KVPTMXFLHLIPWRWELDQDVS---NNHLFSPFGGGARLCPGS 208
Query: 453 ILALPILALALGRLVQNF 470
LA AL L + F
Sbjct: 209 HLARLEXALFLHIFITRFRWEALADEHP 262
AI919813 1357 Pine Lambda Zap Xylem library Pinus taeda cDNA clone
C1_PL11CSUH, mRNA sequence
Length = 653
53% identical to CYP84
Score = 67.9 bits (163), Expect = 9e-13
Identities = 41/104 (39%), Positives = 58/104 (55%), Gaps = 7/104 (6%)
Frame = -3
Query: 397 VARDPAVWKD-----PLEFRPERFLEEDVDMKGHDFRLLPFGSGRRVCPGAQLGINLAAS 451
+ R+P W+D P E ++ ++D+ +F +PFG+GRR CPG QLG+ +
Sbjct: 540 IGRNPKSWEDXESSKPREVSWKKXGXAELDVNVQNFEWIPFGAGRRGCPGQQLGMLVVEX 361
Query: 452 MLGHLLHHFCWTPPEGMK-PEEIDMGEN-PGLVTYMRTPIQAVVSPRLPSH 500
+ LLH F W PE M EE+DM E GL + AV +PRL H
Sbjct: 360 GMAQLLHCFNWRLPEKMNGQEELDMSERFNGLTLPRAHELVAVPTPRLSVH 208
gb|AI812861.1|AI812861 20H12 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 508
Score = 59.3 bits (141), Expect = 4e-10
Identities = 35/92 (38%), Positives = 57/92 (61%), Gaps = 4/92 (4%)
Frame = -2
53% to 77A1 N-term
Query: 4 LLIIPISLVTLWLGYTLYQRLRFK-LPPGPRPWPVVGNLYDI---KPVRFRCFAEWAQSY 59
+L++ ISLV L L L++ R++ LPPGP WP++GNL + R ++ + Y
Sbjct: 291 VLLLVISLVFLRL--VLWKIWRYRNLPPGPPAWPILGNLLQMGFASGAFERSVQKFHERY 118
Query: 60 GPIISVWFGSTLNVIVSNSELAKEVLKEHDQLLADR 95
GPI ++W GS +++++ ELA E L + L +DR
Sbjct: 117 GPIFTMWLGSRPLLMIASQELAHEALIQKGSLFSDRP 10
probable N-terminal of a CYP77 from pine
MAILSFLQAVLLLVISLVFLRLVLWKIWRYRNLPPGP
PAWPILGNLLQMGFASGAFERSVQKFHERYGPIFTMWLGSRPLLMIASQE
LAHEALIQKGSLFSDRPPA
gb|AA220912|AA220912 27 Pinus radiata somatic embryogenic library Pinus radiata cDNA
clone pPRE59A 3'
Length = 508
Score = 29.7 bits (65), Expect = 0.32
Identities = 16/32 (50%), Positives = 16/32 (50%)
Frame = +1
Query: 522 VDLSEVLKLSCEMARPLHCVPVTRVPFAKFSD 553
V VLKLS E H V TR PF KF D
Sbjct: 64 VXXXXVLKLSXEXVXLFHXVSGTRXPFGKFXD 159
gb|AI813156.1|AI813156 23F12 Pine Lambda Zap Xylem library Pinus taeda cDNA, mRNA
sequence
Length = 456
55% to 78A6
Score = 143 bits (356), Expect = 2e-35
Identities = 75/144 (52%), Positives = 102/144 (70%)
Frame = +1
Query: 45 YIALLIVAVCAVLVSWLSPGGCAWAGRHKRGRVAIPGPKGWPIIGSLMDMSVGLPHRKLE 104
++ +L++A+ VL+SWLSPGG AW G KR + IPGP G PI+G+L M L H++L
Sbjct: 19 FLLVLLIAMAYVLLSWLSPGGPAWGGSLKR-KPTIPGPMGLPILGNLTVMC-SLAHQRLA 192
Query: 105 SLARLHGAKQLMSFSLGCTPAVITSDPEVARELLTSPHFANRPLKQSAQQLLFGRAIGFA 164
LA+ A++LM+FS+G T AVI S PEVA+ +L S FA+RP+K+SA QL+F AIGFA
Sbjct: 193 YLAQACNAQKLMAFSMGFTRAVICSQPEVAKAILNSSAFADRPIKESAYQLMFDTAIGFA 372
Query: 165 PNGGYWRLLRRIASAHLFAPRRIA 188
P YW+ L RIA+ HLF+ RI+
Sbjct: 373 PYSEYWKNLMRIAALHLFSVPRIS 444
complete translation 55% to 78A6
AFCNTWFLLVLLIAMAYVLLSWLSPGGPAWGGSLKRKPTIPGPMGLPILG
NLTVMCSLAHQRLAYLAQACNAQKLMAFSMGFTRAVICSQPEVAKAILNS
SAFADRPIKESAYQLMFDTAIGFAPYSEYWKNLMRIAALHLFSVPRISGL
RI
gb|AI725351.1|AI725351 1217 PtIFG2 Pinus taeda cDNA clone 9312r, mRNA sequence
Length = 656
similar to 90A 40%
Query: 24 SKLRGKKLKLPPGPIPIPIFGN----WLQVGDDLNHRNLVDYAKKF-GDLFLLRMGQRNL 78
S ++L LPPG P+ G + + + + + D K++ D+F+ +
Sbjct: 132 SNKXNQELHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQA 311
Query: 79 VVVSSPDLTKEVLLTQGVEFGSRTRNVVFDIFTGKGQDMVFTVYGEHWRKMRRI 132
V+ S P K VLL +G F + + + G + +V+G+ RK+ I
Sbjct: 312 VISSDPQFNKYVLLNEGRFFQAHYPXALKALIRDYG---LLSVHGDLQRKLHGI 464
probable first 164 amino acids of a CYP90 like P450 from pinus
MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQE
LHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG
IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRK
LHGITVNLLKFEKLKFDFMEEI
>BG318892.1 NXPV_021_B08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_021_B08 5'.
Length = 570
CYP90 like sequence
Query: 1 MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
Query: 61 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374
Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEEI 164
ALKALI DYGLLSVHGDLQRKLHGI VNLL+FE+LKFDFMEEI
Sbjct: 375 KALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEI 506
15 MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374
375 KALKALIGDYGLLSVHGDLQRKLHGIAVNLLRFERLKFDFMEEIQNLVHSTID
>BG318205.1 NXPV_011_G08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_011_G08 5'.
Length = 556
CYP90 like sequence
Query: 1 MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
Query: 61 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374
Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEE 163
ALKALI DYGLLSVHGDLQRKLHGI NLL FE+LKFDFMEE
Sbjct: 375 KALKALIGDYGLLSVHGDLQRKLHGIAXNLLXFERLKFDFMEE 503
>BI202885.1 NXPV_091_G09_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_091_G09 5'.
Length = 496
Query: 1 MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
MADQISLLLVVFTAAVALLHLIYRWWNAQRG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAQRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
Query: 61 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYP 120
YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFG QAVISSDPQFNK+VL NEGRFFQAHYP
Sbjct: 195 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGSQAVISSDPQFNKHVLQNEGRFFQAHYP 374
Query: 121 XALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDF 160
ALKALI DYGLLSVHGDLQRKLHGI NLL K F
Sbjct: 375 KALKALIXDYGLLSVHGDLQRKLHGIXXNLLXXXXXKXXF 494
>BG318334.1 NXPV_012_D03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_012_D03 5'.
Length = 326
Query: 1 MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
MADQISLLLVVFTAAVALLHLIYRWWNA RG KRT+N+ N E HLPPGSTGWPLIGETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAXRGQKRTNNEKNPEGHLPPGSTGWPLIGETYS 194
Query: 61 YYRSMTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFN 104
YYRSMT+NRP+QFIDDREKRYDSDIFVSHLFG A ISS N
Sbjct: 195 YYRSMTNNRPKQFIDDREKRYDSDIFVSHLFGXXAXISSXXXXN 326
>BI644007.1 NXPV_138_E03_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_138_E03 5'.
Length = 354
Query: 25 WWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYDSD 84
WWNA G KRT+N+ N E PPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRY SD
Sbjct: 1 WWNAXXGQKRTNNEKNPEGXXPPGSTGWPLIGETYSYYRSMTSNRPRQFIDDREKRYXSD 180
Query: 85 IFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAH
IFVSHLFG QA ISSDPQFNK+VL NEGRFFQAH
Sbjct: 181 IFVSHLFGSQAXISSDPQFNKHVLQNEGRFFQAH
YPXALKALIRDYGLLSVHGDLQR 141
YP ALKALI DYGLLSVHGDLQR
YPKALKALIGDYGLLSVHGDLQR 352
New Pine ESTs 10 Sept. 99
AW043335 ST32B07 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST32B07, mRNA sequence
Length = 593 55% identical to CYP75A3
Score = 123 bits (307), Expect(2) = 1e-33
Identities = 52/126 (41%), Positives = 82/126 (64%)
Frame = +1
Query: 306 NAGTITMIWTMTELTRHPRVMKKLREEIRATLGPNKERITEEDLEKVEYLKLVIKETFRL 365
+ +IT+ W M ELTRHP+++++ +EE+ + +G + + E DL+ + YLK V+KET RL
Sbjct: 10 DTSSITVEWAMAELTRHPKLLRRCQEELDSAVGGEQRTLKESDLQNLPYLKSVVKETLRL 189
Query: 366 HPPGPFLLPRQVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERFANTSIN 425
HPP P L+PR I Y+IPKNA + ++ + + RDP W N EFNP+RF +S++
Sbjct: 190 HPPVPLLIPRXAPXXXXIXXYYIPKNARLXVNAWGMQRDPSVWGNSLEFNPDRFMVSSVD 369
Query: 426 YKGQHY 431
+G +
Sbjct: 370 VRGSDF 387
Score = 37.9 bits (86), Expect(2) = 1e-33
Identities = 14/29 (48%), Positives = 24/29 (82%)
Frame = +3
Query: 429 QHYELLPFGAGRRSCPGMTLGITILELGL 457
+ ++++PFGA RR+C GM++GI ++EL L
Sbjct: 378 ERFQVIPFGAXRRTCAGMSMGIRMVELML 464
55% identical to CYP75A3 53% identical to CYP98A1
DTSSITVEWAMAELTRHPKLLRRCQEELDSAVGGEQRTLKESDLQNLPYLKSVVKETLRL
HPPVPLLIPRXAPXXXXIXXYYIPKNARLXVNAWGMQRDPSVWGNSLEFNPDRFMVSSVD
VRGSDFQVIPFGAXRRTCAGMSMGIRMVELML
gb|AW056850.1|AW056850 ST57B04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST57B04, mRNA sequence
Length = 438
Score = 122 bits (304), Expect = 3e-28
Identities = 53/113 (46%), Positives = 80/113 (69%)
Frame = +2
Query: 385 GYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERFANTSINYKGQHYELLPFGAGRRSCP 444
GY+IPKNA + ++ + I RDP W P EF+PERFA ++++ +G +E++PFGAGRR C
Sbjct: 2 GYYIPKNARLLVNAWGIQRDPDVWERPLEFDPERFAGSAVDVRGTDFEVIPFGAGRRVCA 181
Query: 445 GMTLGITILELGLLNILYYFDWSLPNGMTIKDIDMEEDGALTIAKKVPLELIP 497
G+++GI +++L L ++L+ FDWSLP G +++DM E LT+ K VPL P
Sbjct: 182 GVSMGIRMVQLMLASLLHSFDWSLPEGQLPENLDMAEAYGLTLQKAVPLLAAP 340
65% to CYP75A3
GYYIPKNARLLVNAWGIQRDPDVWERPLEFDPERFAGSAVDVRGTDFEVIPFGAGRRVCA
GVSMGIRMVQLMLASLLHSFDWSLPEGQLPENLDMAEAYGLTLQKAVPLLAAP
gb|AW042602.1|AW042602 ST23G12 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST23G12, mRNA sequence
Length = 478
Score = 112 bits (278), Expect = 4e-25
Identities = 58/142 (40%), Positives = 84/142 (58%), Gaps = 4/142 (2%)
Frame = +1
Query: 316 MTELTRHPRVMKKLREEIRATLGPNKERITEEDLEKVEYLKLVIKETFRLHPPGPFLLPR 375
+ EL H + K+R E+ A LGP + ITE D ++ YL+ V+KET RL P L+P
Sbjct: 13 IAELVNHQDIQSKVRAELDAVLGPGVQ-ITEPDTTRLPYLQAVVKETLRLRMAIPLLVPH 189
Query: 376 QVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERF----ANTSINYKGQHY 431
+ D ++ GY IP + I ++ + + +P W NPEEF PERF +T N G +
Sbjct: 190 MNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEAN--GNDF 363
Query: 432 ELLPFGAGRRSCPGMTLGITILELGL 457
+ LPFG RRSCPG+ L + +L L +
Sbjct: 364 KFLPFGGWRRSCPGIILALPLLALSI 441
83% to 73A5, 79% to 73A20 (Pinus) exact to 73A23
IAELVNHQDIQSKVRAELDAVLGPGVQITEPDTTRLPYLQAVVKETLRLRMAIPLLVPH
MNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEEFRPERFFEEEKHTEANGNDF
KFLPFGGWRRSCPGIILALPLLALSI
gb|AW043075.1|AW043075 ST29A04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST29A04, mRNA sequence
Length = 601
Score = 93.6 bits (229), Expect = 2e-19
Identities = 58/142 (40%), Positives = 80/142 (55%), Gaps = 1/142 (0%)
Frame = +3
Query: 26 SKWKLPPGPTGLPIIGNLHQAWKLLHSSFHKLSLEHGPVMLLRFGVVP-MVVISSKEAAK 84
+K +LP GP LPIIGNLH KL H + LSL++GP M LR G M+VISS + A+
Sbjct: 129 NKRRLPSGPVQLPIIGNLHLLGKLPHQALSALSLKYGPFMSLRLGSSTLMLVISSGDMAE 308
Query: 85 QVLKSRDLETCSRPKLVANGLFTRNFKDIAFAQYGEDWREMKKLVGLELFNPKKHKFFRY 144
+ LK+ D SR A T N I FA YG WR+M+K+ L+L + K+ F+
Sbjct: 309 EFLKTHDQCFASRAPSTAAKCLTYNCSGIVFAPYGPYWRQMRKVCVLQLLSSKRIDSFKX 488
Query: 145 IREEEGDLLVKKLSKSSQTQTLV 167
RE ++ L +QTQ ++
Sbjct: 489 TREXGVSXMI--LXIINQTQXIL 551
55% to 71D16
NKRRLPSGPVQLPIIGNLHLLGKLPHQALSALSLKYGPFMSLRLGSSTLMLVISSGDMAE
EFLKTHDQCFASRAPSTAAKCLTYNCSGIVFAPYGPYWRQMRKVCVLQLLSSKRIDSFKX
TREXGVSXMILXIINQTQXIL
gb|AW010433.1|AW010433 ST06G04 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST06G04, mRNA sequence
Length = 637
Score = 88.2 bits (215), Expect = 9e-18
Identities = 53/123 (43%), Positives = 74/123 (60%), Gaps = 8/123 (6%)
Frame = +3
Query: 383 IQGYHIPKNAHIKISTYAIGRDPKCWTNPEEFNPERF---ANTSINYKG-QHYELLPFGA 438
I GY IP+N + ++ +AIGRDPK W + E F PERF + ++ G Q+ L FG
Sbjct: 6 ESCNILGYEIPQNTCVIVNLWAIGRDPKSWEDAETFKPERFMERVGSEVDANGDQNLGWLAFGT 197
Query: 439 GRRSCPGMTLGITILELGLLNILYYFDWSLP-NGMTIKDIDM---EEDGALTIAKKVPLE 494
GRR CPG LG+ L LG+ +L+ F+W LP + M K+ +M E+ T+ K L
Sbjct: 198 GRRRCPGEQLGMLALXLGVAQLLHCFNWRLPFDDMNGKNQEMEMTEKHNGFTLPKADELC 377
Query: 495 LIPTLPASLCI 505
IPT P C+
Sbjct: 378 AIPT-PRLECV 407
51% to CYP84
ILGYEIPQNTCVIVNLWAIGRDPKSWEDAETFKPERFMERVGSEVDANGDQNLGWLAFGT
GRRRCPGEQLGMLALXLGVAQLLHCFNWRLPFDDMNGKNQEMEMTEKHNGFTLPKADELC
AIPTPRLECV
gb|AW010774.1|AW010774 ST12C06 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST12C06, mRNA sequence
Length = 491
Score = 63.6 bits (152), Expect = 2e-10
Identities = 39/147 (26%), Positives = 66/147 (44%), Gaps = 5/147 (3%)
Frame = +2
Query: 310 ITMIWTMTELTRHPRVMKKLREEIRATL----GPNKERITEEDLEKVEYLKLVIKETFRL 365
I + W L +HP V + + E+R L G N T E++ +++YL + ET RL
Sbjct: 20 IALSWLFWLLIQHPAVEENILSELRQILVSRRGSNPGGFTVEEVRQMQYLHAALSETLRL 199
Query: 366 HPPGPFLLPRQVMSDIEIQGYHIPKNAHIKISTYAIGRDPKCW-TNPEEFNPERFANTSI 424
+P P + ++ G + + ++ Y++GR W + EF PER+ +
Sbjct: 200 YPSLPINYKEVIEDEVLPDGARLKRGTNLIFPIYSMGRTXXIWGEDCREFKPERWLKEGV 379
Query: 425 NYKGQHYELLPFGAGRRSCPGMTLGITILELG 456
Y+ F A R C G + E+G
Sbjct: 380 FVAEGGYKYPVFSAXPRVCLGKDFAFFVHEMG 475
52% to 86C1
IALSWLFWLLIQHPAVEENILSELRQILVSRRGSNPGGFTVEEVRQMQYLHAALSETLRL
YPSLPINYKEVIEDEVLPDGARLKRGTNLIFPIYSMGRTXXIWGEDCREFKPERWLKEGV
FVAEGGYKYPVFSAXPRVCLGKDFAFFVHEMG
gb|AW043205.1|AW043205 ST30E11 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST30E11, mRNA sequence
Length = 587
Score = 60.9 bits (145), Expect = 1e-09
Identities = 39/122 (31%), Positives = 59/122 (47%), Gaps = 4/122 (3%)
Frame = +2
Query: 19 LFKKLLPSKWKLPPGPTGLPIIGNLHQ---AWKLLHSSFHKLSLEHGPVMLLRFGVVPMV 75
LF K P K LPPGP G P++GNL Q + K L + P+ L+ G M+
Sbjct: 179 LFLKSRPPKKHLPPGPRGWPLVGNLFQVVFSGKPFIYVIRDLREKFSPIFTLKMGQKTMI 358
Query: 76 VISSKEAAKQVLKSRDLETCSRPKLVANGL-FTRNFKDIAFAQYGEDWREMKKLVGLELF 134
+I+ + A + L SRP + F+ N + + YG WR +++ + EL
Sbjct: 359 IITCPKVAHEALIENGALFASRPPDTPTKMFFSENKCSVNSSLYGSVWRXLRRNMVSELL 538
Query: 135 NPKKHK 140
NP + K
Sbjct: 539 NPSRMK 556
61% to CYP77A1
LFLKSRPPKKHLPPGPRGWPLVGNLFQVVFSGKPFIYVIRDLREKFSPIFTLKMGQKTMI
IITCPKVAHEALIENGALFASRPPDTPTKMFFSENKCSVNSSLYGSVWRXLRRNMVSELL
NPSRMK
New hits 2/19/02
>BI398025.1 NXPV_113_E11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_113_E11 5'.
Length = 524
CYP90 like
Query: 344 LTWQDYKTMDFTQCVIDETLRLGGIAIWLMREAKEDVSYQD-YVIPKGCFVVPFLSAVHL 402
L W DY+++ FTQCVI ETLR+G + RE KED+ + +VIP+G V FL+ HL
Sbjct: 14 LNWDDYQSLKFTQCVIHETLRVGNFGPGVFRETKEDIKTKGGFVIPRGWTVYVFLTGTHL 193
Query: 403 DESYYKESLSFNPWRWQK----RNWRTSPFYCPFGGGTRFCPGAELARLQIALFLHYLIA 458
DE Y+ +L F+PWRWQ + +P + PFGGG R CPG LA++++ALFLH +
Sbjct: 194 DEKYHSSALKFDPWRWQPHLQDQELLKNPSFMPFGGGARLCPGMHLAKMELALFLHNFVT 373
Query: 459 CFRWTQLKEDRISFFPSARLVNGFKIQLNRRD 490
FRW L++D+IS+FP RL+ G I+L R+
Sbjct: 374 KFRWEALQDDKISYFPFPRLIKGLXIRLRLRE 469
14 LNWDDYQSLKFTQCVIHETLRVGNFGPGVFRETKEDIKTKGGFVIPRGWTVYVFLTGTHL 193
194 DEKYHSSALKFDPWRWQPHLQDQELLKNPSFMPFGGGARLCPGMHLAKMELALFLHNFVT 373
374 KFRWEALQDDKISYFPFPRLIKGLXIRLRLRE 469
>BG318000.1 NXPV_008_D01_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_008_D01 5'.
Length = 534
CYP720 like CYP85 clan
Query: 45 LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
LPPGSTGWPLIGET S++R + S +PRQFI +RE+RY +IF +LFG + V+S D +
Sbjct: 137 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQEREQRY-GEIFRXNLFGRSRIVVSVDXE 313
Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFME 162
FNK+VL +EGR F +YP L+ LI YGLLSVHGDLQRKLHG VNLL DFME
Sbjct: 314 FNKHVLQHEGRQFXXNYPKPLRNLIGKYGLLSVHGDLQRKLHGAAVNLLXXXXXSVDFME 493
Query: 163 EI 164
+I
Sbjct: 494 DI 499
>BI077317.1 NXPV_095_E11_F NXPV (Nsf Xylem Planings wood Vertical) Pinus
taeda cDNA clone NXPV_095_E11 5'.
Length = 260
CYP90 like
Query: 1 MADQISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYS 60
MADQISLLLVVFTAAVALLHLIYRWWNAQR KRT+N+ N E HLPPGSTG L ETYS
Sbjct: 15 MADQISLLLVVFTAAVALLHLIYRWWNAQRXQKRTNNEKNPEGHLPPGSTGXXLXXETYS 194
Query: 61 YYRSMTSNRPRQFIDDREKRYD 82
YYRSMT N P+QFI DREKRYD
Sbjct: 195YYRSMTXNXPKQFIHDREKRYD 260
>BE049731.1 NXNV_143_A08_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_143_A08 5'.
Length = 456
CYP720 like cyp85 clan
Query: 45 LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
LPPGSTGWPLIGET S++R + S +PRQFI +RE+RY +IF S+LFG + V+S DP+
Sbjct: 126 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDPE 302
Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
FNK+VL + R FQ +YP L+ LI YGLLSVHGDL KLHG VNLL
Sbjct: 303 FNKHVLQHXXRQFQXNYPKPLRNLIGKYGLLSVHGDLXXKLHGAXVNLL 449
> BG040616.1 NXSI_113_B06_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_113_B06 5'.
Length = 443
CYP720 like cyp85 clan
Query: 45 LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
LPPGSTGWPLIGET S++R + S +PRQFI +RE+RY +IF S+LFG + V+S D +
Sbjct: 109 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDXE 285
Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
FNK+VL +E R FQ +YP L+ LI YGLLSVHGDL LHG VNLL
Sbjct: 286 FNKHVLQHEXRQFQXNYPKPLRNLIGKYGLLSVHGDLXXXLHGAAVNLL 432
> BE582456.1 NXCI_033_F03_F NXCI (Nsf Xylem Compression wood Inclined) Pinus
taeda cDNA clone NXCI_033_F03 5'.
Length = 446
Query: 45 LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQ 102
LPPGSTGWPLIGET S++R + S +PRQFI +RE+RY +IF S+LFG + V+S DP+
Sbjct: 123 LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRY-GEIFRSNLFGRSRIVVSVDPE 299
Query: 103 FNKYVLLNEGRFFQAHYPXALKALIRDYGLLSVHGDLQRKLHGITVNLL 151
FNK+VL + R FQ +YP L+ LI YG LSVHGD KLHG VNLL
Sbjct: 300 FNKHVLQHXXRQFQXNYPKPLRNLIGKYGXLSVHGDXXXKLHGAXVNLL 446
MTETLTDSWLVGLLCLVLGFLLLQLYKLVWGASSRAYK
LPPGSTGWPLIGETISFFRGINSTAQPRQFIQERERRYGEIFRSNLFGRSRIVVSVDPE
FNKHVLQHEGRQFQANYPKPLRNLIGKYGLLSVHGDLQKKLHGAAVNLL
Rferlsvdfmedi from BI416628.1 Scots pine
AW056514 Related but different seq
81 MAETSVTYSWLVGFVCFVLATILLQLYRIVDSRGYNLPPGSRGWPLIGESLGFMREI 251
252 NSGSQPRRFIXDRELRYGEIFXSNLFGRSRLIVSVXXEFNKHVLQXXXRLFXANXPRPFR 431
432 N 434
AW011172 Related but different seq
52 MGETLHSLLVGLVCFALGMLLLELYKLVWRVDSRGYKLPPGSTGLPLIGETISFFRGIN 228
229 STDQPRRYIQEREKRYGEIFKSNLFGKISDCCVRGFKVQQTCPAARRQEFQANYPKPPPN 408
409 PNRKFWLLACTG 444
> AW011225.1 ST18B12 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST18B12.
Length = 389
CYP90 like different from AA740068
Query: 218 GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMRLYNRE 275
GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPM LYNRE
Sbjct: 3 GGGARLCPGSHLARLELALFLHIFITRFRWEALADEHPSYFPLPYLAKGFPMCLYNRE 176
>BF609170.1 NXSI_041_B05_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_041_B05 5'.
Length = 504
CYP90 like
Query: 162 EEIDTKGXX-IPKGWVVFAFLLATHLDEXXXXXXXTFNPWRWEL---DQDVSNNHLFSPF 217
E++ TKG IPKGW V+ FL THLDE TFNPWRW+ DQ++S N F PF
Sbjct: 4 EDVKTKGGFVIPKGWTVYVFLTGTHLDEKYHSSALTFNPWRWQQLLQDQELSKNPSFMPF 183
Query: 218 GGGARLCPGSHLARLELALFLHIFITRFR 246
GGGARLCPG HLA+LELALFLH F+T+FR
Sbjct: 184 GGGARLCPGMHLAKLELALFLHNFVTKFR 270
>BI416628.1 hasp001xk19f Heterobasidion annosum - Scots pine infection stage
subtraction cDNA library (hasp) Pinus
sylvestris/Heterobasidion annosum cDNA clone
hasp001xk19f.
Length = 470
CYP85 clan
Query: 68 NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKAL 126
+RPR +I +REKRY +IF S+LFG + V+S DP+FNK+VL +EGR FQA+YP L+ L
Sbjct: 7 SRPR-YIQEREKRY-GEIFRSNLFGRSRIVVSVDPEFNKHVLQHEGRQFQANYPKPLRNL 180
Query: 127 IRDYGLLSVHGDLQRKLHGITVNLLKFEKLKFDFMEEI 164
I +GLL ++GDLQ+KLHG VNLL+FE+L DFME+I
Sbjct: 181 IGKFGLLFLYGDLQKKLHGAAVNLLRFERLSVDFMEDI 294
>AW056514.1 ST51B01 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST51B01.
Length = 435
CYP85 clan
Query: 44 HLPPGSTGWPLIGETYSYYRSMTS-NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDP 101
+LPPGS GWPLIGE+ + R + S ++PR+FI DRE RY +IF S+LFG + ++S
Sbjct: 186 NLPPGSRGWPLIGESLGFMREINSGSQPRRFIXDRELRY-GEIFXSNLFGRSRLIVSVXX 362
Query: 102 QFNKYVLLNEGRFFQAHYP 120
+FNK+VL R F A+ P
Sbjct: 363 EFNKHVLQXXXRLFXANXP 419
> BM428379.1 NXRV_015_D05_F NXRV (Nsf Xylem Root wood Vertical) Pinus taeda cDNA
clone NXRV_015_D05 5'.
Length = 413
63% to 85A2
Query: 5 ISLLLVVFTAAVALLHLIYRWWNAQRGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRS 64
I+L + + +ALL WN R K+ LPPG+ GWPL GET + R
Sbjct: 35 IALGFIFLCSIIALLR-----WNEVRYGKKG---------LPPGTMGWPLFGETPDFLRY 172
Query: 65 MTSNRPRQFIDDREKRYDSDIFVSHLFGIQAVISSDPQFNKYVLLNEGRFFQAHYPXALK 124
+QFI +R+ RY D+F +H+ G VIS+DP N+Y+LLN R ++
Sbjct: 173 -----GQQFIKNRKARY-GDLFKTHILGCPTVISTDPALNRYILLNXXRGLIPGXXQSML 334
Query: 125 ALIRDYGLLSVHGDLQRKL 143
++ + +V DL + +
Sbjct: 335 DILGKXXIAAVXXDLHKTM 391
> AW011172.1 ST17E09 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST17E09.
Length = 519
56% to 87A2
Query: 9 LVVFTAAVALLHLIYRWWNAQ-RGXKRTSNKXNQELHLPPGSTGWPLIGETYSYYRSMTS 67
LV F + LL L W RG K LPPGSTG PLIGET S++R + S
Sbjct: 85 LVCFALGMLLLELYKLVWRVDSRGYK-----------LPPGSTGLPLIGETISFFRGINS 231
Query: 68 -NRPRQFIDDREKRYDSDIFVSHLFG-IQAVISSDPQFNKYVLLNEGRFFQAHYPXALKA 125
++PR++I +REKRY +IF S+LFG I + + + FQA+YP
Sbjct: 232 TDQPRRYIQEREKRY-GEIFKSNLFGKISDCCVRGFKVQQTCPAARRQEFQANYPKPPPN 408
Query: 126 LIRDYGLLS
R + LL+
Sbjct: 409 PNRKFWLLA
Query: 135 VHGDLQRKLHGITVNLLKFEKL 156
VHGD Q+KLHG VN K +KL
Sbjct: 435 VHGDFQKKLHGTXVNXFKVQKL 500
> BF778196.1 NXSI_080_C07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_080_C07 5'.
Length = 250
87A2 like
Query: 45 LPPGSTGWPLIGETYSYYRSMTSN-RPRQFIDDREKRYDSDIFVSHLF 91
LPPGSTGWPLIGET S++R + +PRQFI +RE+ Y +I +LF
Sbjct: 109 LPPGSTGWPLIGETISFFRGINXTAQPRQFIQERERXY-GEIXXXNLF 249
> BF610049.1 NXSI_054_B07_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_054_B07 5'.
Length = 380
83% to 85A1
Query: 214 FSPFGGGARLCPGSHLARLELALFLHIFITRFR 246
F+ FGGG+RLCPG L +++++FLH F+TR+R
Sbjct: 7 FTAFGGGSRLCPGKELGLVQISMFLHHFVTRYR 105
> BG625777.1 NXPV_066_A04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_A04 5'.
Length = 453
50% to 90B1
Query: 259 KINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFA 318
KI ++IE R Q+ A N +L +LL+E+S E +ADFI+ LLFAG+ET+S++M FA
Sbjct: 3 KIYESIEDRRQHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHETSSRSMSFA 173
Query: 319 VYFLTHCPKAMTQLQEEHD-------RLAGGMLTWQDYKTMDFTQCVIDETLRLGGIAIW 371
+ FLT CP+A+ +L+ EHD L L W DY+++ FTQCVI ETLR+G
Sbjct: 174 IKFLTDCPRALEELKAEHDALLKRKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPG 353
Query: 372 LMREAKED
+ RE KED
Sbjct: 354 VFRETKED
> BG832634.1 NXPV_077_F04_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_077_F04 5'.
Length = 436
Query: 259 KINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFA 318
KI ++IE R Q+ A N +L +LL+E+S E +ADFI+ LLFAG+ET+S++M FA
Sbjct: 3 KIYESIEDRRQHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHETSSRSMSFA 173
Query: 319 VYFLTHCPKAMTQLQEEHD-------RLAGGMLTWQDYKTMDFTQCVIDETLRLGGIAIW 371
+ FLT CP+A+ +L+ EHD L L W DY+++ FTQCVI ETLR+G
Sbjct: 174 IKFLTDCPRALEELKAEHDALLKXKGNLKNQKLNWDDYQSLKFTQCVIHETLRVGNFGPX 353
Query: 372 LMREAK
+ RE K
Sbjct: 354 VFRETK
> AW225716.1 ST71A02 Pine TriplEx shoot tip library Pinus taeda cDNA clone
ST71A02.
Length = 234
Query: 251 QARKEIIRKINKTIEKRLQNKAASDTAGNGVLGRLLEEESLPNESMADFIINLLFAGNET 310
+AR+ +I+KI ++IE R + A N +L +LL+E+S E +ADFI+ LLFAG+ET
Sbjct: 7 KAREFLIKKIYESIEDRXXHPEA---VHNDLLTKLLKEDSFSEEIIADFILFLLFAGHET 177
Query: 311 TSKTMLFAVYFLTHCPKAM 329
+S+ M FA+ LT CP+A+
Sbjct: 178 SSRXMSFAIKXLTDCPRAL 234
> BG039994.1 NXSI_104_E01_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_104_E01 5'.
Length = 553
74% to 98A3
Query: 285 LLEEESLPNESMADFIINLLFAGNETTSKTMLFAVYFLTHCPKAMTQLQEEHDRLAGG-- 342
L E+ L +++ + +++ AG +TT+ T+ +A+ L P+ + QEE DR+ G
Sbjct: 111 LQEKYDLSEDTIIGLLWDMITAGMDTTAITVEWAMAELVRNPRIQQKAQEEIDRVVGRDR 290
Query: 343 MLTWQDYKTMDFTQCVIDETLRL-GGIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVH 401
++ D+ + + QC+ E LRL + L +A ++V Y IPKG V + A+
Sbjct: 291 VMNETDFPHLPYLQCITKEXLRLHPPTPLMLPHKATQNVKXXGYDIPKGSNVHXXVWAIA 470
Query: 402 LDESYYKE 409
D + +K+
Sbjct: 471 RDPAVWKD 494
> AI725152.1 1051 PtIFG2 Pinus taeda cDNA clone 9047r.
Length = 395
Like CYP720
Query: 281 VLGRLLEEESLPNESMADFIINLLFAGNETTSKTMLFAVYFLTHCPKAMTQLQEEHDRL 339
+L +LL ++S+ +E DFI+ LLFAG+ T+SK A FLT CP+ L + H+R+
Sbjct: 84 LLTKLLXQDSISDEITCDFILFLLFAGHXTSSKXXTXATKFLTTCPR----LIDSHERI 248
> BM158852.1 NXLV_040_D06_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
clone NXLV_040_D06 5'.
Length = 401
57% to 98A3
Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRWT 463
PFG GT+ CPG +LA L + L L+ CF WT
Sbjct: 23 PFGAGTKVCPGEQLAMLLVQFPLAXLLHCFYWT 121
> AW870094.1 NXNV_124_A07_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_124_A07 5'.
Length = 375
60% to 76C2
Query: 326 PKAMTQLQEEHDRLAGG--MLTWQDYKTMDFTQCVIDETLRLGGIA-IWLMREAKEDVSY 382
P + + Q E D + G + D + + Q V+ E LRL A + + R A
Sbjct: 8 PTLLKKAQAELDEVVGREKRMEESDIAKLPYLQAVVKEVLRLHPAAPLIIPRRADNSAEI 187
Query: 383 QDYVIPKGCFVVPFLSAVHLDESYYKESLSFNPWRWQ--KRNWRTSPF-YCPFGGGTRFC 439
YV+P+ V + + D + +KE L F P R+ K ++R F PFG C
Sbjct: 188 GGYVVPENTQVFVNIWGIVRDPNVWKEPLKFKPERFLXCKTDYRXXDFELIPFGAXXXIC 367
>AW290673.1 NXNV044H02F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV044H02 5'.
Length = 374
81% to 51A2
Query: 343 CIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHIVATSPAFANRLPHIFKDPDT 402
CIKEALRLHPPLI+L+R++H DF+VTA+DGK Y IPKGH+VATSPAFANRLPHIFK+PDT
Sbjct: 1 CIKEALRLHPPLIVLLRSNHRDFTVTAKDGKDYVIPKGHVVATSPAFANRLPHIFKNPDT 180
Query: 403 YDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPF 462
YDP+RF PGREEDK GAFSYI+FGGGRHGCLGEPFAYLQIKAIW+HLLRNFE L+SP
Sbjct: 181 YDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFEXXLISPX 360
Query: 463 PEID 466
E D
Sbjct: 361 XEXD 372
CYP51 N-term
MDFIQNIYVQAGLLILATLVLAKIVSSILGFGSSKNLPPMVPAWPIVGGLMKFLKGPIVMLR
EEYPKLGNVFTIKLLIKNVTFLIGPEVSQHFFKXPESDL
CYP51 C-term
LXHDVLAXXDVLYRCIKEALRLHPPLIVLLRSNHRDFTVTAKDGKDYVIPKGHVVATSPAFANRLPHIFKNPDT
YDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAIWTHLLRNFELELISP
FPEIDWNAMVVGVKDKVMVRYRRRPLSVD*
>CYP51A2 Arabidopsis missing part
SQQEVYQFNVPTFGPG
VVFDVDYSVRQEQFRFFTEALRVNKLKGYVDMMVTEAEDYFSKWGESGEVDIKVELERLI
ILTASRCLLGREVRDQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDRAREKLSEI
FAKIIGSRKRSGKTENDMLQCFIESKYKDGRQTTESEVTGLLIAALFAGQHTSSITSTWT
GAYLMRYKEYFSAALDEQKNLIAKHGDK
>gi|11604806|gb|BF517195.1|BF517195 NXSI_011_E08_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_011_E08 5'.
Length = 538
Score = 212 bits (540), Expect = 9e-56
Identities = 97/110 (88%), Positives = 104/110 (94%)
Frame = +3
Query: 379 KGHIVATSPAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPF 438
KGH+VATSPAFANRLPHIFK+PDTYDP+RF PGREEDK GAFSYI+FGGGRHGCLGEPF
Sbjct: 3 KGHVVATSPAFANRLPHIFKNPDTYDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPF 182
Query: 439 AYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRYKRRQLS 488
AYLQIKAIW+HLLRNFELEL+SPFPEIDWNAMVVGVK VMVRY+RR LS
Sbjct: 183 AYLQIKAIWTHLLRNFELELISPFPEIDWNAMVVGVKDKVMVRYRRRPLS 332
>gi|8179218|gb|AW984874.1|AW984874 NXNV_119_G10_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_119_G10 5'.
Length = 292
Score = 186 bits (471), Expect = 9e-48
Identities = 84/96 (87%), Positives = 89/96 (92%)
Frame = +3
Query: 387 PAFANRLPHIFKDPDTYDPERFSPGREEDKAAGAFSYIAFGGGRHGCLGEPFAYLQIKAI 446
PAFANRLPHIFK+PDTYDP+RF PGREEDK GAFSYI+FGGGRHGCLGEPFAYLQIKAI
Sbjct: 3 PAFANRLPHIFKNPDTYDPDRFVPGREEDKVGGAFSYISFGGGRHGCLGEPFAYLQIKAI 182
Query: 447 WSHLLRNFELELVSPFPEIDWNAMVVGVKGNVMVRY 482
W+HLLRNFELEL+SPFPEIDWNAMVVGVK M RY
Sbjct: 183 WTHLLRNFELELISPFPEIDWNAMVVGVKDXXMXRY 290
>gi|7363541|gb|AW626637.1|AW626637 NXNV067F11 Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV067F11 5'.
Length = 257
Score = 145 bits (366), Expect = 1e-35
Identities = 64/85 (75%), Positives = 76/85 (89%)
Frame = +2
Query: 329 IDHDILSEMDVLYRCIKEALRLHPPLIMLMRASHSDFSVTARDGKTYDIPKGHIVATSPA 388
+ HD+L+ DVLYRCIKEALRLHPPLI+L+R++H DF+VTA+DGK Y IPKGH+VATSP
Sbjct: 2 LXHDVLAXXDVLYRCIKEALRLHPPLIVLLRSNHKDFTVTAKDGKDYVIPKGHVVATSPX 181
Query: 389 FANRLPHIFKDPDTYDPERFSPGRE 413
FANRLPHIF +PDTYDP+RF PGRE
Sbjct: 182 FANRLPHIFXNPDTYDPDRFVPGRE 256
>gi|11778796|gb|BF610290.1|BF610290 NXSI_057_D10_F NXSI (Nsf Xylem Side wood Inclined) Pinus taeda cDNA
clone NXSI_057_D10 5'.
Length = 351
Score = 120 bits (302), Expect = 3e-28
Identities = 61/99 (61%), Positives = 77/99 (77%)
Frame = +2
Query: 6 ENKLLKTGLVIVATLVIAKLIFSFFTSDSKKKRLPPTLKAWPPLVGSLIKFLKGPIIMLR 65
+N ++ GL+I+ATLV+AK++ S S K LPP + AWP +VG L+KFLKGPI+MLR
Sbjct: 59 QNIYVQAGLLILATLVLAKIVSSILGFGSSKN-LPPMVPAWP-IVGGLMKFLKGPIVMLR 232
Query: 66 EEYPKLGSVFTVNLVHKKITFLIGPEVSAHFFKASESDL 104
EEYPKLG+VFT+ L+ K +TFLIGPEVS HFFK ESDL
Sbjct: 233 EEYPKLGNVFTIKLLIKNVTFLIGPEVSQHFFKXPESDL 349
> AW888136.1 NXNV_129_F04_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA
clone NXNV_129_F04 5'.
Length = 242
85% to 98A3
Query: 403 DESYYKESLSFNPWRWQKRNWRTSPF---YCPFGGGTRFCPGAELARLQIALFLHYLIAC 459
D + +K+ ++F P R+ + + PFG G R CPGA+L + L +L+
Sbjct: 4 DPAVWKDPVTFRPERFLEEDVDIKGHDYRLLPFGAGRRICPGAQLGINLVQSMLGHLLHH 183
Query: 460 FRW 462
F W
Sbjct: 184 FVW 192
> BE187484.1 NXNV_98_G10_F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV_98_G10 5'.
Length = 614
87% to 73A5
Query: 352 MDFTQCVIDETLRLG-GIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHLDESYYKES 410
+ + Q V+ ETLRL I + + D Y IP ++ + + + +K
Sbjct: 21 LPYLQAVVKETLRLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNP 200
Query: 411 LSFNPWRWQKRNWRTSPF-----YCPFGGGTRFCPGAELARLQIALFLHYLIACF 460
F P R+ + T + PFG G R CPG LA +AL + L+ F
Sbjct: 201 EEFRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPLLALSIGRLVQNF 365
> BM158467.1 NXLV_034_H02_F NXLV (Nsf Xylem Late wood Vertical) Pinus taeda cDNA
clone NXLV_034_H02 5'.
Length = 585
68% to 78A5
Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRW 462
PFG G R CPG L + L++ L+ F+W
Sbjct: 41 PFGAGRRVCPGKALGLATVNLWVAKLLHHFQW 136
> BG625848.1 NXPV_066_G08_F NXPV (Nsf Xylem Planings wood Vertical) Pinus taeda
cDNA clone NXPV_066_G08 5'.
Length = 251
71% to 98A3
Query: 337 DRLAGG--MLTWQDYKTMDFTQCVIDETLRLGG-IAIWLMREAKEDVSYQDYVIPKGCFV 393
DR+ G ++ D+ + + QC+ E LRL + L +A ++V Y IPKG V
Sbjct: 5 DRVVGRDRVMNETDFPHLPYLQCITKEALRLHPPTPLMLPHKATQNVKIGGYDIPKGXNV 184
Query: 394 VPFLSAVHLDESYYKESLSFNP 415
+ A+ + +K +F P
Sbjct: 185 HVNVWAIAR XXAVWKXPXTFKP 250
> AW754540.1 PC03D04 Pine TriplEx pollen cone library Pinus taeda cDNA clone
PC03D04.
Length = 464
Query: 431 PFGGGTRFCPGAELARLQIALFLHYLIACFRW 462
PF G R CPGA L + L L L F W
Sbjct: 59 PFSAGKRKCPGAPLGTCFVLLALARLFHSFHW 154
> AW290024.1 NXNV009G04F Nsf Xylem Normal wood Vertical Pinus taeda cDNA clone
NXNV009G04 5'.
Length = 438
84% to 73A5
Query: 354 FTQCVIDETLRLG-GIAIWLMREAKEDVSYQDYVIPKGCFVVPFLSAVHLDESYYKESLS 412
+ Q V+ ET L I + + D Y IP ++ + + + +K
Sbjct: 2 YLQAVVKETXXLRMAIPLLVPHMNLHDAKLGGYDIPAESKILVNAWWLANNPANWKNPEE 181
Query: 413 FNPWRWQKRNWRTSPF-----YCPFGGGTRFCPGAELARLQIALFLHYLIACF 460
F P R+ + T + PFG G R CPG LA +AL + L+ F
Sbjct: 182 FRPERFFEEEKHTEANGNDFKFLPFGVGRRSCPGIILALPLLALSIGRLVXNF 340