ࡱ> ceb  tSbjbjҬҬ 4Ɯ_Ɯ_tK`````tttt4t,,,,,,,,$s) n*`,,,,,*``,,?,:`,`,,pFfBU0   `(,,,,,,,**,,,,,,, ,,,,,,,,,X &:  ESTIMATE 90 BACTERIAL P450 GENES This is a collection of the newest bacterial P450s many have not been named yet. AE000101 Rhizobium sp. NGR234 plasmid pNGR234a same seq as Z68203 CYP127 MSDLRRKRVKTNPIPDHVPPALVRHFSLFTSPGMAPTPNGDPHA AVACVHDDGPPIFYSPSNTRDGRGTWVITRARDQRRVLEDTETFSSHRSIFASALGEH WPVIPLELDPPAHGVFRALLNPLFSSRRVLALEPTIHARAGALIDCIAKEKTSCDVMK DFALPFTFSVFLSFLGLSQRRSEVLVGWVSDLLHGNAEKRRAAARSVVAFIDEMAAMR RKSPAVDFMTFVVQAKIEGRSLTEEEVRGIGVLFLVAGLDTVAAAIGFDMAYLARNPK HQELLRNEPARLGLAAEELLRAYSTVQIIRVATKDIEFEGVPIREGDYVSCPAMIANR DPSEFKCPNTIDLARQDNQHTAFGYGPHLCHGAHLARREIVIGLREWLARIPAFRIKE GTAPITHGGHVFGISNIILTWA AF071144 Streptomyces glaucescens cytochrome P450 LLIAGHETTTSMIALSTLLLLDRPELPAELRNDPDLMPAAVDEL LRVLSVADSIPLRVAAEDIELSGRTVPADDGVIALLAGANHDPEQFDDPERVDFHRTD NHHVAFGYGMHQCLGQNL AF071146 Micromonospora inyoensis cytochrome P450 LLIAGHETTSHMISLGVTALLEHPDQLAALQNDLTLLPEAVEEL LRYLSIADYVPSRVALEDVVIGGTVIRAGEGVVPLLAAADWDPKVFDNPGTLDIHRGN RRHVAFGYGVHQCLGQNL AF071147 Streptoalloteichus hindustanus cytochrome LLIAGHETTANMLALGAFALLEHPEQLAELRANPDLMPGAVEEL MRYLSIVHIGPVRTAVADVEIEGQLIRAGESVTVSVPAANWDPAKFPEPERLDLTRRT SGHLAFGHGVHQCLRQNL AF071148 Amycolata autotrophica cytochrome P450 LLIAGHETTSHMISLGVTALLERPDQLAALQNDLTLLPEAVEEL LRYLSIADYVPSRVALEDVVIGGTVIRAGEGVVPLLAAADWDPKVFDNPGTLDIHRGN RRHVAFGYGVHQCLGQNL U17130 Rhodococcus erythropolis ORF6' gene CYP116 /gene="thcB" /standard_name="cytochrome P-450" /note="first member of the new cytochrome P-450 family CYP116" /function="degradation of thiocarbamate herbicides" /product="ThcB" MTVDHAPEGVKSPTGCPVSGMAADFDPFRGAYQVDPSSSLRQAR KDEPVFFSPLLDYWVVTRYEDIKQIFKTPSVFSPSITVDQITPISDEALQILGSYQFA AGRMLVNEDEPIHTERRRLLMQPFEADNVATLEPKIREVVNTYLDRVIKDGRADLIGD LLYEVPCIVALIFLGVPDEDIETCRQYGMQQTLFTWGHPTGDEQTRVATGMGKFWEFA GGLVDKLKADPNAKGWIPHAIEMQRQHPDLFDDNYLQNIMFGGVFAAHETTTNATGNA FRTLLENRSSWDEICADPTLIPKAIEECLRYSGSVVAWRRKAVVDTTVGEVDIPAGGR LLIVMASANRDDSMFPEPDDFDIHRGNAQRHLTFGIGSHTCLGATLARLEMKVFLEEV SRRLPHMSLVAGQEFSYLPNTSFRGPEHVLVEWDPQQNPVPADRP" AB000735 Nocardioides sp. 32% with B.s. 109B1 MSSVLAPESDVDLFAGEVLADPYPIYAALRSLGPVVWLPRHGFY VVVRYAEAREVLNDHERFVSGRGVGFNQQFNDVRSGSIIASDPPRHDILRSVLNERLG PRALKDTEVMIRSRASDLVAEMAQRRSFDAVKDFAEVFPVQVVGELIGLPEGSRGRLL QWANGAFNAFGPSGERTAAGLEAIAEQFDYIRTVANREQLMPDSMGAAVYQAADEGII SEDDCLPLLSAYLTAGMDTTVNALSAMLLLLSTDPDQWQELRSSPSLAPSVVNEVLRI EAPAQLFSRVTATRVDLAGTRIEAGERVAVIYASANRDEKKYPDPDRFDIRRNPAGHL AFGSGLHVCAGQFLAKTELRAVLDALIEQIETMTVGEPVRKINNVLRGLSSLPATFVP VQTSR AF034769 Agrobacterium tumefaciens plasmid pTiC58 /gene="virH1" 77% identical to pinF1 MITSSISGTDQQFQNATQPKELDPDAVPVSRLDSEGHEIFAEWR PKRPFLRREDGVFLVLRADDIFLLGTDPRTRQIETELMLNRGVTRGAVFDLIRYSMLF SNGEVHVKRRSAFAKTFAFRMIDALRPEITKLTEHLWDDVPRVDDFDFAEMYASKLPA LTIASVLGLPFGDAPFFTRLVYNVSRCLSPSWGEDDFPEIEASAVELQDYVRAVVADR SRRISDDFLSCYLKAVREEGTLSPIEEIMQLVFLILAGSDATRNAMVMLPTLLLQNPV VWSSLCHDQSGVAAAVEEGLRFEPSVGSFPRLALEDIDLDGYVLPKGSFLALSIMSGL RDERHYEHPQLFDIKRKQMRRHLGFGAGVHRCLGEALARIELQEGLRTLLRRAPSLRV TGDWPRMIGHGGARRATGMTVNLGVDR" U84350 Amycolatopsis orientalis hypothetical hydrox /product="hypothetical hydroxylase d" 91% IDENTICAL TO AJ223998 GENE 1 ISTYDPPEHTRLRKMLTPEFTVRRIRRMEPAIQSLVDDRLDMLE AEGSPADLQGLFADPVGAHALCELLGIPRDDQREFVRRIRRNADLSRGLKARAADSAA FNRYLDNLIARQRADPDDGLLGTIVREHGGNVTDEELRGLCTALILGGVETVAGMIGF GVLALLQHPGQIPLLFEGPEKADRVVNELVRYLSPVQAPNPRLAIKDVVVDGQLIKAG DYVLCSVLMANRDEALTPDPNVFDANRTAVSDVGFGHGIHYCVGAALARSMLRTAYQT LWRRFPGLRLAVPVEEVKYRSAFVDCPDQVPVTW AJ223998 Amycolatopsis orientalis cosmid PCZA361 2 genes gene 1 N-TERM IS FROM END OF AJ223999 MAHGIDQHGIDQHGIDQHGIDQHGIDQHGIDQHGIDQHSIDQHG IDQHGMEQVAPLLQEPANFQMRTGCDPHEENFDLRAHGPLVRLVGDSSTQLGRDYVWQ AHGYDVVRKILGDHENFTTRPQFTHAKSDAHVEAQFVGQISTYDPPEHTRLRKMLT 91% IDENTICAL TO U84350 PEFTVRRIRRMEPAIQGLIDDRLDMVEAEGPPADLQGLFADPVGALALCELLGIPRDD QREFVRRIRRNTDLSRGLKARAADSAAFNRYLDNLISRQRKDPDEGFLGMIVREHGDN VTDEELKGLCTALILGGVETVAGMIGFGVLALLENPDQIQLLFAGPEKADRVVNELVR YLSPVQAPNPRLAIKDVVIDGQLIKAGDYVLCSVLMANRDEALTPNPNVLDANRAAVS DVGFGHGIHYCVGAALARSMLRMAYQTLWQRFPGLRLAVPIAEVKYRSAFVDCPDQVP VTW gene 2 34% WITH B.S. Z99119 (107H) MQTTTAVGDLGNPDLYTTLDRHARWRELAARDAMVWSEPGSSPT GFWSVFSHRACAAVLAPSAPFTSEYGMMIGFDRDHPDKSGGQMMVVSEQEQHRKLRRL VGPLLSRAAARKLSERVRTEVSGVLDQVLDGGVCDVATAIGPRIPAAVVCEILGVPAE DEDMLIELTNHAFGGEDELFDGMTPRQAHTEILVYFDELITARRERPADDLVSTLVTD DELTIDDVLLNCDNVLIGGNETTRHAITGAVHALATVPGLLTGLQDGSADVDTVVEEV LRWTSPAMHVLRVSTDDVTINGQDLPAGTPVVAWLPAANRDPAEFDDPDTFLPGRKPN RHITFGHGMHHCLGSALARIELSVVLRVLAERVSRVELVKEPAWLRAIVVQGYAELSA RFTGR AJ223999 Amycolatopsis orientalis cosmid PCZA363 31325..32500 GENE 1 42% WITH AJ223999 GENE 2 AND U84350 /note="similar to P450 related oxidase/hydroxylase" MFAENNAVRGTEIHRREQFDPGPELRSLMAEGPMSILEADDPAE GRTGWLATGYDEIRQVLGSDKFSAKLLYGGTVAGRIWPGFLNQYDPPEHTRLRRMLTS AFTVRRMQGFRPRIELIVEATLDDIEATGGPVDFVPRFAWPIATTVTCDFIGIPRDDQ ADLSRALLASRSERTGKRRVAAGNKFWTYMSQVAAQARRDPGDNMFGAVVREHGDAIT DAELLGVAAFIIGAAGDQVARFLAAGAWLIAEHPEQFAVLRDKPDTIPDWLNEVARYL TSDEKTTPRIALEDVYIGDQLVKAGDAVTCSLLAANRRNFPAPEDQFDITRERPAHVT FGHGIHHCLGRPLAEMVFRTAITALTRRFPTLRLAEPGREIKLGPPPFDVEVLLLDW 32520..33740 GENE 2 42% WITH AJ223999 GENE 1 /note="similar to P450 related oxidase/hydroxylase" 53% WITH U84350 MVAQELSQSGDDDPRPLHIRRQDLDPADELLAAGALTRVTVGSG ADAETSWMATTHGAVRQVMGDHKSFSTRRRWHQRDEIGGTGIFRPRELVGNLMDYDPP EHTRLRQKLTPGFTLRKMQRMQPYIEQIVADRLDAMEQAGSPADLIEFVADEVPGAVL CELIGVPRDDRAMFMQLCHGHLDASRSQKRRAAAGEAFSRYLLAMIARERKDPGEGLI GAVIAEYGDEATDEELRGFCVQVMLAGDDNISGMIGLGVLALLRHPEQIAAFQGDEQS AQRAVDELIRYLTVPYAPTPRIAMQDVIVASQMIKKGESVICSLPAANRDPALVPDPN RLDVTREPVPHVAFGHGVHHCLGAALSRLELRTVYTALWQRFPTLRLADPAKETNFRL TTPAYGVTSLLVAW 33791..>34244 GENE 3 /note="similar to P450 related oxidase/hydroxylase" THIS GENE IS IDENTICAL TO AJ233998 GENE 1 N-terminal MAHGIDQHGIDQHGIDQHGIDQHGIDQHGIDQHGIDQHSIDQHG IDQHGMEQVAPLLQEPANFQMRTGCDPHEENFDLRAHGPLVRLVGDSSTQLGRDYVWQ AHGYDVVRKILGDHENFTTRPQFTHAKSDAHVEAQFVGQISTYDPPEHT AF040571 Amycolatopsis mediterranei 2 genes 65% WITH AF040570 GENE 3 PVPLSIVSRDRVSIQRLACKVFGETRSGPPGRASFVLIAPRRPQ TVHKGHNSEHCQVGGCSTGRPLPTVRNRACRTRAGRPRRHQEGMAMTATAKPSAKPVD LFSPEVVADPFGWYARLREEPLPHTGTLNLGTMMGGPDMWLATRYEDVLTVLTDPRFL TNPPADSPLADIRDGVFKAMNFPEDLIPWMANKLNTADGEDHTRLRKLVSYALTARRV NALRPRVEKITEDLFDRMAEQGKDGSPVDIVEEFCFPLPVTVICELVGIDEPDRAAWH AWGNAMATMDGPKIPPALRECIALSRELMARRRVEPKDDLVTALVQAQADDPGRVSDD EIVGILFSLVTAGHQTTTYLIGNSVLFLLEQPEQLARLKAEPALWAQAVRELQRLGPL QFTQARFPSEDVELGGVTIPRGTPVAPLLLAANTDPRKFPEPDKLVVDRLSVANEMHL SFGKGIHRCLGQHLAYQEAEVALHGLFTRFPDLALAVPRDEIPWILRPAFTRTKTLPL KLA 48% WITH M31939 105C1 49% WITH 105B1 MTTKVTENAPSTESLRSPLPPEFVRREDPFHVPPALVAVSERGP VARATLAAGDPFWLVSGYEEARAVLSDPRFSSDRFQYHPRFKELSPEFAFPAPSLMIC ELLGVRYEDRAEFQQRASALLQMNAPVAEAVKNADALRAFMQALVTDKRANPAGDIIS GLIHHAGADPALTDDELINIANLLLIAGYDTTASMLGLGIFVLLQRPAQLATLRDDPS RIADAVEGLLRYLSVVNPGIFRFAKEDLEFAGEHIPAGSTVVVSVVATNRDARHWPDP DLDLTRPRGPHLAFGHGVHQCLGQQLARMEMQAGYAELLRRLPNVRLAVPPEEVPLRN DMLTYGVHSLPIAWDAP AJ223012 Amycolatopsis mediterranei genes encoding rifamycin polyketide synthases, ORFs 1 to 5 IDENTICAL TO AF040570 GENE 1 Sbjct: 156 DPPAVFDSLREERPLAKMVYPDGHVGWIVSSYELVREVLSDLR-FSHSCEVGHFPVTHQG 332 Sbjct: 333 QVIPTHPLIPGMFIHMDPPEHTRYRKLLTGEFTVRRASRLIPRAEAVAAEQIEVMRAKGA 512 Sbjct: 513 PADVVMDFAKPLVLRMLGELVGLPYEERDRYVPAVTLLHDAEADPAEAAAAYEVAGKFFD 692 Sbjct: 693 EVIERRRQRPQDDLISSLVT-----EDLTQEELRNIVTLLLFAGYETTEGALATGVFALL 857 Sbjct: 858 HHTDQLAALRAEPEKLDAAIEELLR-YLTVNQYHTYRTALEDVKLEGELIKKGDTVTVSL 1034 Sbjct: 1035PAANRDPAKFGCPAELDIERDTSGHVAFGFGIHQCLGQNLARIELRAGFTALLRAFPELR 1214 Sbjct: 1215LAVPADEVPLRLKGSVFSVKKLPVSW 1292 AF040570 Amycolatopsis mediterranei rifamycin biosy 3 genes /note="ORF0; similar to Streptomyces Sp. ChoP" GENE 1 IDENTICAL TO AJ223012 41% TO 105C1 MTDAISFEVPWDRTDKFDPPAVFDSLREERPLAKMVYPDGHVGW IVSSYELVREVLSDLRFSHSCEVGHFPVTHQGQVIPTHPLIPGMFIHMDPPEHTRYRK LLTGEFTVRRASRLIPRAEAVAAEQIEVMRAKGAPADVVMDFAKPLVLRMLGELVGLP YEERDRYVPAVTLLHDAEADPAEAAAAYEVAGKFFDEVIERRRQRPQDDLISSLVTED LTQEELRNIVTLLLFAGYETTEGALATGVFALLHHTDQLAALRAEPEKLDAAIEELLR YLTVNQYHTYRTALEDVKLEGELIKKGDTVTVSLPAANRDPAKFGCPAELDIERDTSG HVAFGFGIHQCLGQNLARIELRAGFTALLRAFPELRLAVPADEVPLRLKGSVFSVKKL PVSW complement(67462..68673) GENE 2 /note="ORF4" /product="cytochrome P450 monooxygenase" 50% WITH X63601 105D1 MTETVAFPQDRTCPYQPAPGYEPLADRRPIAEVTLYDGRKAWAV TGHELARKLLCDPRISSDRTNPAWPMVSAAAAVEFTDVQQKVIKLLTALVGVDGPAHR ARRKLVQAGFTVKRIDSLRPKIQALVDRQIDEMIAEGAPVDLLKKFASPVPLIALCDL LGIPHEDQDYFEKKAHQILFGPDAGGAYDELMAYLTKLIDKAEQNPGEEGFLQALLAE RDPSSDVDHEEILQMFLIVLVTGHDTTSSSIGLGAFTLLQHPERLAELRADPSLMPAA VDELTRFVVVPDGLQRVAADDIEVDADTTIRKGDGVFFLFSLINRDEEAYEDGDRLDW HRPTFRDHLTYGFGTHQCAGMNHARAIMEIAFRRLIDRLPGLRLAVPADEVRVKPGDA FQGLAELPVTW complement(68704..69969) GENE 3 65% WITH AF040570 GENE 1 /product="cytochrome P450 monooxygenase" MTTTAETSAEPVDLFSPEVVADPWGAHAAVREAPTLQIGGFMGG PPMYLAARYEDVRQVLMDQRFQCNPLADSPAEDIRNGVFRHLDMPEELIPWLKNLINA SDGEEHARLRKLVSYALTAHRVGKFRPRVEELTAELLDKLEAAGGDGTPIDLVESFCY PLPVTVICELVGVDHEDRPQWRAWSDAMATLDGKRLPEEVRKTLAAARGMIERRRAEP KDDMVTALVEAQAKNPGIATDDELVGVLFSLVTAGHQTTTYLIGNSVLALHEHPDQLA RLKADPSLWPQAVRELQRLGPIQFGQPRFATEDIEVGGTVIPRGMPVVPLIMAANSDP RKFPEPEKLQIDRLAVGSESHLGFGKGIHRCLGQHVAYLEAEVALRGLYTRFPDMTLA VPREEIPWILRPGFTRTKDLPVRLARPAS D87924 Actinomadura hibisca polyketide synthase gene 44% WITH 107G1 X86780 (check for two genes) and 107D1 MPSSKDAPTVDPRPDVTPAFPFRPDDPFQPPCEHARLRASDPVA KVVLPTGDHAWVVTRYADVRFVTSDRRFSKEAVTRPGAPRLIPMQRGSKSLVIMDPPE HTRMRKIVSRAFTARRVEGMRAHVRDLTSGFVDEMVEHGPPADLIAHLALPLPVTVIC EMLGVPPEDRPRFQDWTDRMLTIGAPALAQADEIKAAVGRLRGYLAELIDAKTAAPAD DLLSLLSRAHADDGLSEEELLTFGMTLLAAGYHTTTAAITHSVYHLLREPSRYARLRE DPSGIPAAVEELLRYGQIGGGAGAIRIAVEDVEVGGTLVRAGEAVIPLFNAANRDPEV FADPEELDLGRTDNPHIALGHGIHYCLGAPLARLELQVVLETLVERTPALRLAIDDAD ITWRPGLAFARPDALPIAW Z97193 Mycobacterium tuberculosis cosmid Y180 44% with 107A1 MKDKLHWLAMHGVIRGIAAIGIRRGDLQARLIADPAVATDPVPF YDEVRSHGALVRNRANYLTVDHRLAHDLLRSDDFRVVSFGENLPPPLRWLERRTRGDQ LHPLREPSLLAVEPPDHTRYRKTVSAVFTSRAVSALRDLVEQTAINLLDRFAEQPGIV DVVGRYCSQLPIVVISEILGVPEHDRPRVLEFGELAAPSLDIGIPWRQYLRVQQGIRG FDCWLEGHLQQLRHAPGDDLMSQLIQIAESGDNETQLDETELRAIAGLVLVAGFETTV NLLGNGIRMLLDTPEHLATLRQHPELWPNTVEEILRLDSPVQLTARVACRDVEVAGVR IKRGEVVVIYLAAANRDPAVFPDPHRFDIERPNAGRHLAFSTGRHFCLGAALARAEGE VGLRTFFDRFPDVRAAGAGSRRDTRVLRGWSTLPVTLGPARSMVSP Z97345 Mycobacterium tuberculosis cosmid Y25C11 34% with 107B1 MRRSPKGSPGAVLDLQRRVDQAVSADHAELMTIAKDANTFFGAE SVQDPYPLYERMRAAGSVHRIANSDFYAVCGWDAVNEAIGRPEDFSSNLTATMTYTAE GTAKPFEMDPLGGPTHVLATADDPAHAVHRKLVLRHLAAKRIRVMEQFTVQAADRLWV DGMQDGCIEWMGAMANRLPMMVVAELIGLPDPDIAQLVKWGYAATQLLEGLVENDQLV AAGVALMELSGYIFEQFDRAAADPRDNLLGELATACASGELDTLTAQVMMVTLFAAGG ESTAALLGSAVWILATRPDIQQQVRANPELLGAFIEETLRYEPPFRGHYRHVRNATTL DGTELPADSHLLLLWGAANRDPAQFEAPGEFRLDRAGGKGHISFGKGAHFCVGAALAR LEARIVLRLLLDRTSVIEAADVGGWLPSILVRRIERLELAVQ AL022021 Mycobacterium tuberculosis sequence v049 34% with 127 MTTPGEDHAGSFYLPRLEYSTLPMAVDRGVGWKTLRDAGPVVFM NGWYYLTRREDVLAALRNPKVFSSRKALQPPGNPLPVVPLAFDPPEHTRYRRILQPYF SPAALSKALPSLRRHTVAMIDAIAGRGECEAMADLANLFPFQLFLVLYGLPLEDRDRL IGWKDAVIAMSDRPHPTEADVAAARELLEYLTAMVAERRRNPGPDVLSQVQIGEDPLS EIEVLGLSHLLILAGLDTVTAAVGFSLLELARRPQLRAMLRDNPKQIRVFIEEIVRLE PSAPVAPRVTTEPVTVGGMTLPAGSPVRLCMAAVNRDGSDAMSTDELVMDGKVHRHWG FGGGPHRCLGSHLARLELTLLVGEWLNQIPDFELAPDYAPEIRFPSKSFALKNLPLRWS AL022022 Mycobacterium tuberculosis sequence v023 38% with 107B1 MTEAPDVDLADGNFYASREARAAYRWMRANQPVFRDRNGLAAAS TYQAVIDAERQPELFSNAGGIRPDQPALPMMIDMDDPAHLLRRKLVNAGFTRKRVKDK EASIAALCDTLIDAVCERGECDFVRDLAAPLPMAVIGDMLGVRPEQRDMFLRWSDDLV TFLSSHVSQEDFQITMDAFAAYNDFTRATIAARRADPTDDLVSVLVSSEVDGERLSDD ELVMETLLILIGGDETTRHTLSGGTEQLLRNRDQWDLLQRDPSLLPGAIEEMLRWTAP VKNMCRVLTADTEFHGTALCAGEKMMLLFESANFDEAVFCEPEKFDVQRNPNSHLAFG FGTHFCLGNQLARLELSLMTERVLRRLPDLRLVADDSVLPLRPANFVSGLESMPVVFT PSPPLG AL021942 Mycobacterium tuberculosis H37Rv complete 40% with Z96800 Mt Y63 MSGTSSMGLPPGPRLSGSVQAVLMLRHGLRFLTACQRRYGSVFT LHVAGFGHMVYLSDPAAIKTVFAGNPSVFHAGEANSMLAGLLGDSSLLLIDDDVHRDR RRLMSPPFHRDAVARQAGPIAEIAAANIAGWPMAKAFAVAPKMSEITLEVILRTVIGA SDPVRLAALRKVMPRLLNVGPWATLALANPSLLNNRLWSRLRRRIEEADALLYAEIAD RRADPDLAARTDTLAMLVRAADEDGRTMTERELRDQLITLLVAGHDTTATGLSWALER LTRHPVTLAKAVQAADASAAGDPAGDEYLDAVAKETLRIRPVVYDVGRVLTEAVEVAG YRLPAGVMVVPAIGLVHASAQLYPDPERFDPDRMVGATLSPTTWLPFGGGNRRCLGAT FAMVEMRVVLREILRRVELSTTTTSGERPKLKHVIMVPHRGARIRVRATRDVSATSQA TAQGAGCPAARGGGPSRAVGSQ AL008609 Mycobacterium leprae cosmid B1788 like 107B1 42% TRLRKLVSKAFAPKVVQALEGDIAALVDSLLDKGAAAGQFDVIA DLAFPLAVAVICRLLGVPYEDAPEFGRVSALLVQSVDPFITITGEPPEATEERLRAGV WLRDYLEQLVKCRRGTPGEDLISRLIELDESGDQLTEEEIIATCGLLLVAGHETTVNL IANAVLAMLRNPSQWKALSSNPQRAPLVVEETLRYDPAIHLIGRVAAKDMTIGQTTLT EGDTMVLLLAAANRDPAVYSRPDEFDPDRPSSRHLAFAVGSHFCLGAALARLEATVTL SAISARFPQVQLAGELVYKPNVAMRGMSALPVQV CYP? Mycobacterium tuberculosis GenEMBL Z83866 coding region 23158-24636 29% with CYP26 MATIHPPAYLLDQAKRRFTPSFNNFPGMSLVEHMLLNTKFPEKK LAEPPPGSGLKPVVGDAGLPILGHMIEMLRGGPDYLMFLYKTKGPVVFGDSAVLPGVA ALGPDAAQVIYSNRNKDYSQQGWVPVIGPFFHRGLMLLDFEEHMFHRRIMQEAFVRSR LAGYLEQMDRVVSRVVADDWVVNDARFLVYPAMKALTLDIASMVFMGHEPGTDHELVT KVNKAFTITTRAGNAVIRTSVPPFTWWRGLRARELLENYFTARVKERREASGNDLLTV LCQTEDDDGNRFSDADIVNHMIFLMMAAHDTSTSTATTMAYQLAAHPEWQQRCRDESD RHGDGPLDIESLEQLESLDLVMNESIRLVTPVQWAMRQTVRDTELLGYYLPKGTNVIA YPGMNHRLPEIWTDPLTFDPERFTEPRNEHKRHRYAFTPFGGGVHKCIGMVFDQLEIK TILHRLLRRYRLELSRPDYQPRWDYSAMPIPMDGMPIVLRPR CYP? Mycobacterium tuberculosis GenEMBL Z95150 cosmid cY164 from Sanger Centre coding region 29289-30488 35% with 107D1 MTSTSIPTFPFDRPVPTEPSPMLSELRNSCPVAPIELPSGHTAW LVTRFDDVKGVLSDKRFSCRAAAHPSSPPFVPFVQLCPSLLSIDGPQHTAARRLLAQG LNPGFIARMRPVVQQIVDNALDDLAAAEPPVDFQEIVSVPIGEQLMAKLLGVEPKTVH ELAAHVDAAMSVCEIGDEEVSRRWSALCTMVIDILHRKLAEPGDDLLSTIAQANRQQS TMTDEQVVGMLLTVVIGGVDTPIAVITNGLASLLHHRDQYERLVEDPGRVARAVEEIV RFNPATEIEHLRVVTEDVVIAGTALSAGSPAFTSITSANRDSDQFLDPDEFDVERNPN EHIAFGYGPHACPASAYSRMCLTTFFTSLTQRFPQLQLARPFEDLERRGKGLHSVGIK ELLVTWPT ADD 3 PSEUDOMONAS AERUGINOSA GENES CYP118 fragments RLGSGIVEQRISVLSIIALADPXXXXXXXDETVWENI XXXXXXRLLMGDELFTXXXXESNWGKAHN (THIS FRAGMENT MATCHES 12/19 RESIDUES IN CYP102A1 AND CYP102A2 SQHDDILDIMLYSADPSTGEQLDTDNV VNQILTLLVSGSQTLANAIAFALHYLLSIHHDIAAQTRREI RCLRRVVDATLRLWSXXXXXXRQARRDTTLGNGXXFPKGXXXXXXXXXXXX THE CYP118 FRAGMENT BELOW IS 50% IDENTICAL TO yetO (CYP102A2) of B. subtilis RADAWGPDANEFNPDRVLPEICRKLPYTYILFGTGLRTCIGRRFALHEMALELTMIVHQYD CYP102A2 P450 part (N-terminal half) MKETSPIPQPKTFGPLGNLPLIDKDKPTLSLIKLAEEQGPIFQI HTPAGTTIVVSGHELVKEVCDEERFDKSIEGALEKVRAFSGDGLFTSWTHEPNWRKAH NILMPTFSQRAMKDYHEKMVDIAVQLIQKWARLNPNEAVDVPGDMTRLTLDTIGLCGF NYRFNSYYRETPHPFINSMVRALDEAMHQMQRLDVQDKLMVRTKRQFRYDIQTMFSLV DSIIAERRANGDQDEKDLLARMLNVEDPETGEKLDDENIRFQIITFLIAGHETTSGLL SFATYFLLKHPDKLKKAYEEVDRVLTDAAPTYKQVLELTYIRMILNESLRLWPTAPAF SLYPKEDTVIGGKFPITTNDRISVLIPQLHRDRDAWGKDAEEFRPERFEHQDQVPHHA YKPFGNGQRACIGMQFALHEATLVLGMILKYFTLIDHENYELDIKQTLTLKPGDFHIS VQSRHQEAIHADVQAAEKAAPDEQKEKTE X82490 Fusarium oxysporum (a fungal P450) MAPMLRPLVYRFIPERARIKDQWTKGRKRVMASMRERQEKGGNL EDPPTMLDHLSNGRNEHIADDVELQLLHQMTLIAVGTVTTFSSTTQAIYDLVAHPEYI TILREEVESVPRDPNGNFTKDSTVAMDKLDSFLKESQRFNSPDLSMSNLKNYKLCESL TGHSNLPTRTIADMKLPDGTFVPKGTKLEINTCSIHKDHKLYENPEQFDGLRFHKWRK APGKEKRYMYSSSGTDDLSWGFGRHACPGRYLSAINIKLIMAELLMNYDIKLPDGLSR PKNIEFEVLASLNACANA C. crescentus at TIGR >gcc_1109 Length = 6908 Minus Strand HSPs: Score = 254 (89.4 bits), Expect = 3.1e-21, P = 3.1e-21 Identities = 93/298 (31%), Positives = 159/298 (53%), Frame = -2 Query: 24 VRRED-PFHVPPALVAVSER--GPVARATLAAGDPFWLVSGYEEARAVLSDPRFSSDRFQ 80 +RRE P+ P L ++ER G V R L +P L +G E + FSS Sbjct: 1060 LRREHMPYFTPSYLRGLTERVKGEVTRL-LDEMEPL-LANGAE----IDMVEHFSS---- 911 Query: 81 YHPRFKELSPEFAFPAPSLMICELLGVRYEDRAE--FQQRASALLQMNAPVAEAV----K 134 P F L P P L + Y +RA+ ++A+A +Q + + V Sbjct: 910 VLPLFT-LCEILGVP-PEDRPKFLTWMHYLERAQDLAVKQANAPMQPTLELMQFVMDFNN 737 Query: 135 NADALRAFMQALVTDKRANPAGDIISGLIHHAGADPALTDDELINIANLLLI-AGYDTTA 193 N + + + + ++ +R +P D+++ I A D A+ DE ++ + LL++ AG DTT Sbjct: 736 NVEEMFEYGRTMLHKRREDPKEDLMTA-IARAQLDGAVLPDEYLDGSWLLIVFAGNDTTR 560 Query: 194 SMLGLGIFVLLQRPAQLATLRDDPSRIADAVEGLLRYLSVVNPGIF--RFAKEDLEFAGE 251 + L + +L + P Q L DPS + AV+ +R +V+P ++ R A D+E G+ Sbjct: 559 NTLSGAMRLLTEFPDQKQKLIADPSLLGGAVDEFIR---MVSPVVYMRRTATRDVEVNGQ 389 Query: 252 HIPAGSTVVVSVVATNRDARHWPDPD-LDLTRPR-GPHLAFGHGVHQCLGQQLARMEMQA 309 I G ++ A NRD + +PD LD+TR G H+AFG+G H C+G+++A+++++ Sbjct: 388 LIREGEKAIMYYGAANRDPAMFENPDQLDVTRANAGKHIAFGYGPHTCIGKRVAQIQLEE 209 Query: 310 GYAELLRRLPNV 321 Y ++L R P++ Sbjct: 208 AYRQILARFPDL 173 >gcc_831 Length = 4246 Plus Strand HSPs: Score = 253 (89.1 bits), Expect = 4.7e-21, P = 4.7e-21 Identities = 71/238 (29%), Positives = 127/238 (53%), Frame = +3 Query: 100 MICELLGVRYEDRAEFQQRASALLQMNAPVAEAVKNADALRA-FMQAL-----VTDKRAN 153 M+ L +E+R + + + + +P + +++ +A RA ++ L + ++R N Sbjct: 2946 MLATLFDFPWEERRKLTRWSD--IATASPESGLIESEEARRAELLECLAYFTNLWNERVN 3119 Query: 154 ---PAGDIISGLIHHAGADPALTDDELINIANLLLIAGYDTTASMLGLGIFVLLQRPAQL 210 P D+IS ++ H A + E + LL++ G DTT + L G++ L + P + Sbjct: 3120 LTEPGNDLIS-MLAHGEATRDMPPMEYLGNVILLIVGGNDTTRNSLTGGLYALSKNPQEE 3296 Query: 211 ATLRDDPSRIADAVEGLLRYLSVVNPGIFRFAKEDLEFAGEHIPAGSTVVVSVVATNRDA 270 A LR DP I + V ++R+ + + + R A ED E AG+ I G VV+ V+ NRD Sbjct: 3297 AKLRADPGLIPNMVSEIIRWQTPL-AHMRRTALEDYELAGQTIKKGDKVVMWYVSGNRDD 3473 Query: 271 RHWPDPDLDLT-RPRGP-HLAFGHGVHQCLGQQLARMEMQAGYAELLRRLPNVRLAVPPE 328 + D + RP HL+FG G+H+C+G +LA M+++ + E+L+R P + + P+ Sbjct: 3474 TVIENADQFIVDRPNARRHLSFGFGIHRCVGNRLAEMQLKIVWEEILKRFPKIEVLEEPK 3653 Query: 329 EV 330 V Sbjct: 3654 RV 3659 >gcc_113 Length = 3962 Minus Strand HSPs: Score = 226 (79.6 bits), Expect = 1.7e-18, Sum P(2) = 1.7e-18 Identities = 70/243 (28%), Positives = 114/243 (46%), Frame = -1 Query: 77 DRFQYHPRFKELSPEFAFPAPSLMICELLGVRYEDRAEFQQRASALLQMNAP-------- 128 DR H + + + AF P +I E+LGV D + L P Sbjct: 731 DRMAEHGDRCDFARDVAFLYPLHVIMEVLGVPESDEXRMLKLTQELFGNADPDLNRTGKS 552 Query: 129 VAEAVKNADALRA----FMQ---ALVTDKRANPAGDIISGLIH-HAGADPALTDDELINI 180 V + + D++++ FM A+ D+RANP D+ + + + +P + E ++ Sbjct: 551 VTDVGEGVDSIQSVVMDFMMYFNAITEDRRANPRDDLATLIANGKINGEP-MGHLEAMSY 375 Query: 181 ANLLLIAGYDTTASMLGLGIFVLLQRPAQLATLRDDPSRIADAVEGLLRYLSVVNPGIFR 240 + AG+DTT+S ++ L + P Q A ++ DPS I +E +R+++ V R Sbjct: 374 YIIAATAGHDTTSSTTAGALWALAENPDQFAKVKADPSLIPGLIEESIRWVTPVKH-FMR 198 Query: 241 FAKEDLEFAGEHIPAGSTVVVSVVATNRDARHWPDP-DLDLTRPRGPHLAFGHGVHQCLG 299 A D E G+ I G +++S + NRD + DP + R H+AFG+G H C G Sbjct: 197 TATADAELGGQKIAKGDWIMLSYPSGNRDEAVFEDPFTFRVDRTPNKHVAFGYGAHICFG 18 Query: 300 QQLAR 304 Q LAR Sbjct: 17 QHLAR 3 >gcc_1435 Length = 834 Minus Strand HSPs: Score = 71 (25.0 bits), Expect = 2.5, P = 0.92 Identities = 28/102 (27%), Positives = 40/102 (39%), Frame = -3 Query: 250 GEHIPAGSTVVVSVVATNRDARHW-------PDPDLDLTRPRG--PHLAFGHGVHQCLGQ 300 G + G + +S +R + W PD +D P G L FG G C+G Sbjct: 697 GHAVVPGQIITISPWLIHRHRKLWDAPTAFVPDRFIDQPHPWGIEAFLPFGAGPRVCIGA 518 Query: 301 QLARMEMQAGYAELLRRLPNVRLAVPPEEVPLRNDMLTYGVHSLP 345 A E Q A LL R + L +P+ + +T G P Sbjct: 517 SFALAEAQIVLASLLERF-EIGLVSDRPVIPIAS--ITLGPDHAP 392 "#juv( c  O f g    J K x ( c v w  $ % W X e 7r#^%`LN:u<wh]Vth]VtOJQJ^J`"#juv( c  O f g    J K x gd]Vt ( c v w  $ % W X e 7r#^gd]Vt%`LN:u<wgd]Vtw(KLeQVBHIz{O;uvO;v1lO ; v !C!~!!!!/"j"|"}"""h]Vth]VtOJQJ^J`(KLeQVBHIz{gd]VtO;uvO;v1lgd]VtO ; v 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