1073 sequences + 21 name change notices

note a few (~8) are alleles or different gene models

of the same gene. About 1065 different sequences.

 

Now 1423 CYP entries Jan. 22, 2009 (added A. niger)

29 of these are alleles (v2) or name changes and do not

contribute to the sequence count, which leaves 1394.

 

Added 78 from Mycosphaerella graminicola now 1472

On 1/28/09 10AM count is 1680

 

After much manual annoation of P450s from the Fungal genome database

I have 2003 named P450 sequences in this set. 2/27/2009 DRN

 

CURRENTLY (4/16/2009) THERE ARE 2582 NAMED FUNGAL P450 SEQUENCES

 

On Aug. 11 2009 after putting all these P450s into a speadsheet

There were 2527 named fungal P450s after excluding variants.

There are 164 pseudogenes.

 

 

>CYP51F1 Mgr032 Mycosphaerella graminicola

63% to CYP51F1     Aspergillus fumigatus

MGLLQEVLAQFDAQFGQTSLWKLVGLGFLAFSTLAILLNVLSQLLFRGKLSDPPLVFHWVPFIGSTITYGIDPYKFFFSCREKYGDVFTFILLGKKTTVCLGTKGNDFILNGKLKDVNAEEIYSPLTTPVFGKDVVYDCPNSKLMEQKKFVKYGLTTSALQSYVTLIAAETRQFFDRNNPHKKFASTSGTIDLPPALAELTIYTASRSLQGKEVREGFDSSFADLYHYLDMGFTPINFMLPWAPLPQNRRRDYAQKKMSETYMSIIQKRRESKTGEHEEDMIHNLMQCKYKDGNAIPDKEIAHMMIALLMAGQHSSSATESWITLRLASRPDIQDELLQEQKDMLGVNADGSIKELTYANLSKLTLLNQVVKETLRIHAPIHSILRKVKSPMPIEGTAYVIPTTHTLLAAPGTTSRMDEHFPDCLHWEPHRWDESPSEKYKHLSPTTALGSIAEEKEDYGYGLVSKGAASPYLPFGAGRHRCIGEQFAYVQLQTITATMVRDFKFYNVDGSDNVVGTDYSSLFSRPLSPAVVKWERREEKEEKN

 

>CYP51F1 Mycosphaerella fijiensis

e_gw1.3.1317.1

81% to CYP51F1 Mycosphaerella graminicola

MGLLQDAAALFDAQFGQTATWKLVPLGFSIFFAVSVLLNVLRQLLFRNPNEPPLVFHYVPFIGSTISYGIDPYKFFFACR

QKYGDCFTFILLGKKTTVVLGTKGNVFILNGKLKDVNAEEIYSPLTTPVFGTDVVYDCPNSKLMEQKKFVKYGLTSSALQ

SYVKLITKETKDFFSKDNPSKKFASTHGTVDLPPAMAELTIYTASRSLQGKEVREKFDSSFADLYHDLDMGFTPINFMLP

WAPLPQNRARDRAQKKMAEVYTAIIKERREKGEPTSGEKEQDMIWNLMQCQYKNGQAIPDKEIAHMMIALLMAGQHSSSS

TSCWILLRLASRPDIQDELLQEQKDVLGVNADGSIKELTYADISRLPLLNQVVKETLRLHAPIHSILRQVKSPMPLEGTP

YVVPTTHSLLAAPGATSRMDEHFPEAMLWEPHRWDENPSEKYAHLAPKHVKEGVAEETEDYGYGLVSKGAASPYLPFGAG

RHRCIGEQFAYVQLQTITSEVIRDFKLYNVDGSDKVVGTDYSSLFSRPLSPAVVRWERREKK*

 

>CYP51F1  Erysiphe graminis f. sp. hordei AF052515

MGISESFMFPYLQPLLQLGFGIALASGILSLLLLLTFLNVLKQL

LFKNPNEPPIVFHWIPIIGSTISYGMNPYKFFHESQAKYGNIFTFILLGKKTTVYLGR

QGNNFILNGKLRDVNAEEIYTVLTTPVFGTDVVYDCPNSKLMEQKKFMKAALTTEAFR

SYVPIIQNEVKSFIEKCDDFRKSKGIINIDAVMAEITIYTASHTLQGKEVRDRFDSSL

AVLYHDLDMGFTPINFMLHWAPLPHNRARDHAQRTVAKIYMEIINSRRTQKETDDSNL

DIMWQLMRSSYKDGTPLPDKEIAHMMIALLMAGQHSSSSSSTWIMLWLAARPDITEEL

YQEQLELLGSELPPLKYEDLSKLSLHQNVLKEVLRLHAPIHSILRKVKNPMPVPGTSY

VIPKTHSLLAAPGWTSRDASYFPNPLKWDPHRWDTGSGGVIGTDMEDEKFDYGYGLIS

TGAASPYLPFGAGRHRCIGEQFATVQLVTIMATMVRSFKHNLDGRNSVAETDYSSMF

SRPMQPATIAWEKR

 

>CYP51F1 Uncinocarpus reesii

78% to CYP51F1    Ajellomyces capsulatus

UREG_07804.1

MGVLADLFAGARQACSTQPLWTLALLGLGAFITLSVVVNVLNQILFKNPNEPPVVFHWFPVIGSTITYGIDPYNFFFSCR

EKYGDIFTFILLGKKTTVYLGTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSEALR

SYVTLITREVEQYLENSPAFKGDSGILSVAKVVAEITIYTASRSLQGKEVRDKFDSSFAELYGDLDMGFAAINFMFPWAP

LPHNRKRDRAQQKMAQIYTDIIRERRAAGGEKDSEDMVWNLMSSVYKDGTPVPDIEVAHMMIALLMAGQHSSSSTGSWIV

LRLASRPDILEELYEEQLRVLGPDLPPLTYESLQRLELHSNVIKETLRIHAPIHSILRAVKSPMPVDGTKYVIPTTHNLL

AAPGVASRLPDYFPDPMLWNPHRWEKGGAVNTTGVLDEGAEEKIDYGYGLVSKGANSPYLPFGSGRHRCIGEQFAYVQLG

TITAALVRALKLKKLDGDKEIPNTDYSVSRKGICH

 

>CYP51F1 Coccidioides immitis

89% to CYP51F1 Uncinocarpus reesii

CIMG_07469.2

MGLLANVLTGVSKACSGQSPWTLVLLGICAFLTLSVFVNVLKQVFFKNPNEPPVVFHWFPIIGSTISYGIDPYKFFFSCQ

KKYGDIFTFILLGKKTTVFLGTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSEALR

SYVTLITREVESYLENSPAFQGDSGVFNVAKSVAEITIYTASRSLQGKEVRDKFDSSFAELYADLDMGFAAINFMFPWAP

LPHNRKRDRAQQKMTQVYTDIIRERRKAGGKKDSEDMVWNLMSSVYKDGTPVPDVEVAHMMIALLMAGQHSSASTGSWIV

LRLASRPDILEELYQEQLRVLGPDLPPLTYESLQRLELHSNVIKETLRLHAPIHSILRAVKSPMPVDGTKYVVPTSHNLL

AAPGVPSRMADYFPEPMLWDPHRWEKGGGVNATGVQDEAAEEKVDYGYGLVSKGANSPYLPFGSGRHRCIGEQFAYVQLG

TITAALVRALKLRKLDGDKEVPDTDYSSLFSKPLGNPKVAWEKRKPSSKQ

 

>CYP51F1  Debaryomyces hansenii

75% to CYP51F1   Candida guilliermondii

CAG88416.1

MALADYSIALYNYFTSLAIVQQASIIILFPFIYNLLWQFFYSFRKDRVPLVFHWMPWVGSAVVYGMQPYEFFENCRTKYG

DVFAFVLLGKVMTVYLGPKGHEFVLNSKLQDVSAEEAYTHLTTPVFGKGVIYDCSNSRLMEQKKFAKYALTKDSFRKYVP

KIKEEVLSYFTDSENFNMKGKSSGVVNVMESQPEITIFTASRSLMGDEMRKKFDASFAQLYTDLDKGFTPINFVFPHLPL

PQYRKRDAAQQKISSTYMSLISKRRDTGDIDPSRDLIDSLMTHSTYKDGVRMTDQEIANLLIGVLMGGQHTSASTSAWFL

LHLAENPSLQDEVYEEILSVLKQKNGSFNDLDYDDLQNMPLLNNIIKETLRMHMPLHSIFRKVKNPLLVPNTQYVVPKGH

HVLVSPGYAMTNEKWFPEAAKFKPHRWDETAKATGNEDTVDYGFGAITKGVASPYLPFGGGRHRCIGEQFAYVQLGTILS

TYVYNMKWSLKDGKMPEIDYASMVTLPMEPADICWEKRENCVI

 

>CYP51F1 Pichia anomala AF019903

EFVKGALTRDAFKSYVPQIREEIIHYFQNSKNFKFGERKHGISN

VMATQPEITIFTASRSLMGKQMRERFDESFAGYYSDLDKGFTPINFVFPNLPLPSYRR

RDYAQQKISSTYMGLIKERRENNDIKDRDLIDTLMKTSTYKDGVKMTDQEIANLLIGV

LMGGQHTSAATSAWFLLHLAEKPELQEEIYQEIMKVTGGEDVTYDDLQEMPLVNNVIK

ETLRMHMPLHSIFRKVKNPLPVPGTSYVVPKGHHVLVSPGFAMTNENYFPQPEVYNPH

RWDEDSTSKAAQDSSTVDYGFGAVSKGVSSPYLP

 

>CYP51F1 Pichia stipitis

71% TO CYP51F1    Debaryomyces hansenii

JGI model e_gww1.7.1.351.1

MALLDSAIVAYNYFVSLSLTQQVLIIVFAPFIYNIVWQFLYSLRRDRAPLVFHWIPWFGSAIPFGMAPYKFFEDSRQKYG

DVFAFMLLGRTMTVYLGPKGHEFVFNSKLNETNAEDAYKHLTTPVFGKGVIYDCPNHRLMEQKKFAKYALTRDAFKTYVP

KIREEIISYFKNPEGFNMGEKNSGVANIMKTQPELTIFTASRSLLGDDMRKRFDASFAQLFSDLDKGFTPLNFVFTNLPL

PNYRKRDNAQQKISATYKSLIEKRRATGDVDPNRDLIDSLMTYSTYKDGVKMTDQEIANLLIGILMGGQHTSAATSAWFL

LHLGARPDLQDELFEEVSNILKEKGTSNLNDLSYEDLQNMTLTNNVIKETLRLHMPLHSIFRKVSKLLLVPNTNYTVPAG

HYVLVSPGYTQTSDRFFPNASTFDPHRWDSNNLADKNADEGTVDYGFGAVSKGVSSPYLPFGGGRHRCIGEQFAYVQLGT

ILSAFVYNVKWSLKGEKVPANDFTSMVVLPEEPAEIIWEKRKTCTF

 

>CYP51F1   Aspergillus fumigatus

          GenEMBL AAK73660.1 also XP_749134.1, EAL87096.1

          14-alpha sterol demethylase

          79% to 51F1 A. nidulans

MGLIAFILDGICKHCSTQSTWVLVGIGLLSILAVSVIINVLQQLLFKNPHEPPVVFHWFPFIGSTISYGI

DPYKFFFDCRAKYGDIFTFILLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPN

AKLMEQKKFVKYGLTSDALRSYVPLITDEVESFVKNSPAFQGHKGVFDVCKTIAEITIYTASRSLQGKEV

RSKFDSTFAELYHNLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQAGSKKDSEDMVWN

LMSCVYKNGTPVPDEEIAHMMIALLMAGQHSSSSTASWIVLRLATRPDIMEELYQEQIRVLGSDLPPLTY

DNLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMAVDGTSYVIPTSHNVLSSPGVTARSEEHFPNPLEWN

PHRWDENIAASAEDDEKVDYGYGLVSKGTNSPYLPFGAGRHRCIGEQFAYLQLGTITAVLVRLFRFRNLP

GVDGIPDTDYSSLFSKPLGRSFVEFEKRESATKA

 

>CYP51F1  Aspergillus oryzae

         GenEMBL BAE60239.1

         79% to CYP51F1 Aspergillus nidulans, 60% to CYP51F4

MGILAVILDSVCERCSGSSLWMLSTVALLSILVVSVVINVLRQLLFKNYKEPPLVFHWFPFIGSTISYGM

DPYRFFFNCREKYGDIFTFVLLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPN

AKLMEQKKFVKFGLTSDALRSYVRLITEEVEDFVQKSSALQGPNGVFDVCKTIAEITIYTASRSLQGKEV

RSRFDSTFAELYHDLDMGFAPINFMLPWAPLPHNRKRDAAQKRMTETYMEIIKERRKAGSKKDSEDMVWN

LMSCMYKDGTPVPDEEIAHMMIALLMAGQHSSSSTAAWIVLHLAASPEITEELYQEQLRILGHDMPPLTY

ENLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVEGTPYVIPTSHNVLSSPGVTARSEEHFPDPLEWK

PHRWDEAIAVSSEDEEKVDYGYGLVTKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAALVRLFKFSNLP

GVQTLPDTDYSSLFSKPLGNSKIQFEKREPVTKA

 

>CYP51F1  Aspergillus flavus

100% to CYP51F1  Aspergillus oryzae

AFL2G_06478

MGILAVILDSVCERCSGSSLWMLSTVALLSILVVSVVINVLRQLLFKNYKEPPLVFHWFPFIGSTISYGMDPYRFFFNCR

EKYGDIFTFVLLGKKTTVYLGTKGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSDALR

SYVRLITEEVEDFVQKSSALQGPNGVFDVCKTIAEITIYTASRSLQGKEVRSRFDSTFAELYHDLDMGFAPINFMLPWAP

LPHNRKRDAAQKRMTETYMEIIKERRKAGSKKDSEDMVWNLMSCMYKDGTPVPDEEIAHMMIALLMAGQHSSSSTAAWIV

LHLAASPEITEELYQEQLRILGHDMPPLTYENLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVEGTPYVIPTSHNVL

SSPGVTARSEEHFPDPLEWKPHRWDEAIAVSSEDEEKVDYGYGLVTKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAAL

VRLFKFSNLPGVQTLPDTDYSSLFSKPLGNSKIQFEKREPVTKA*

 

>CYP51F1  Aspergillus niger

         estExt_fgenesh1_pm.C_150008|Aspni1

         84% to CYP51F1

MGLLAVVLDSLCERCSNTSVLVVLGFGLLSLLAVSVIFNILRQLFFKNPNEPPLVFHWFPFIGSTISYGMDPYKFFFDCR

AKYGDIFTFILLGKKTTVYLGTRGNDFILNGKLRDVCAEEVYSPLTTPVFGRNVVYDCPNAKLMEQKKFVKFGLTSDALR

SYVRLITGEVENFVEHSAAFKGSSGVFDVCKTIAEITIYTASRSLQGKEVRSRFDSTFAELYHDLDMGFAPINFMLPWAP

LPHNRKRDAAQKKMTETYMEIIKERRNGGNKKDSEDMVWNLMSCIYKDGTPVPDEEIAHMMIALLMAGQHSSSSTASWIV

LHLARNPQIMEELYAEQIRVLGSDLPPLTYDNLQKLDLHAKVIKETLRIHAPIHSIIRAVKNPMPVDGTPYVIPTSHNVL

SSPGVTARSEEYFPNPLKWDPHRWDETIAASAEEEDQIDYGYGLVSKGTNSPYLPFGAGRHRCIGEQFAYVQLGAITAAL

VRLFKFRNLPNVKDIPETDYSSLFSKPAGKSIIQFEKRATTIKS*

 

>CYP51F1  Aspergillus clavatus NRRL 1

         XM_001273213.1, ACLA_005420

         89% to 51F1 S. fumigatus

         61% to 51F2 A. fumigatus

MGLITDILDGVCKYCSTQSIWMLGGVGLLSLLAVAVVVNVLRQL

LFKNPNEPPLVFHWFPFIGSTISYGIDPYKFFFDCRAKYGDIFTFILLGKKTTVYLGT

KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKFGLTSDALR

SYVHLITDELESFVKSSSAFQGPKGVFDVCKTIAEITIYTASRSLQGKEVRNKFDSTF

AELYHDLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQAGNEKDSED

MVWNLMSCVYKNGVPVPDEEIAHMMIALLMAGQHSSSSTAAWIVLRLATRPDIMEELY

QEQLRVLGSDLPPLTYDSLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMPVEGTPYV

IPTSHNVLSSPGVTARSEEHFADPLEWNPHRWDEHIVENDEDEEKIDYGYGLVNKGTN

SPYLPFGAGRHRCIGEQFAYVQLGTITAGLARLFKFRNLPDIEGIPDTDYSSLFSKPL

GKSVVQFEKREPALKA

 

>CYP51F1  Aspergillus terreus NIH2624

         XM_001212028.1

         ATEG_02850.1

MGVFADIIGTLCEHCSTLSIWALGGAVLLAVFVLSVVINVLRQL

LFKNPHEPPVVFHWVPWIGSTIRYGIDPYKFFFECR AKYGDIFTFVLLGKKTTVYLGT

KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQ KKFVKFGLTSDALR

SYARLITAEVDDFVKNSPTFQQPKGTFDVCKTIAEITIYTASRSLQGKEVRNRFDSTF

AEMYHDLDMGFAPINFVLPWAPLPHNRKRDAAQKKMAETYMEIIKER RQAGGKKDSED

MVWNLMSCVYKDGTP LPDEEIAHMMIALLMAGQHSSSSTASWIVLRLAENPEITEELY

QEQLRVLGSDLPPLTWDSLQKLDLHAKVIKETLRIHAPIHSIMRAVKNPMPVEGTPYV

IPTSHNVLSSPGVTARSEEYFSEPLKWDPHRWDETAAPSTEEEEETIDYGYGLVTKGT

NSPYLPFGAGRHRCIGEQFAYVQLGAITAALVRLFKFSNPPGANKLPDTDYSSLFSKP

LGKSYVQFEKRETNTKA

 

>CYP51F1P  Aspergillus terreus

71% to CYP51F1 Aspergillus terreus

ATEG_10302.1 revised

MGLFAAALDKICKQCSTQAIYMLSGVGVITTLVLSVVINVLIQLSFKNPNEPPVVFHWFPLIGSTISYGTDPYKFFFECRRK (0)

YGDIFTFILLGKKTTVFLGTKGNDFVLNAKLHDLCAE*VYSSLTTPVFGNHVVYDCPNFKPMKQKK (0)

FVKFGLASDALRSYVELITKEVDEYITNANAFKQPRGTIDVGKVIADITIYTASRLLQGKEVREKFDSTFAELYHDLD

MGFAPINFILPWAPLPHNCKRDAAQKKMAQTYMEIIQKRR

*SGGEKNSEDMVWNLMSCVY

KDGTPVPDIEVAHIMIALLMAGQHSSSSTISWIILRLASQPEITEQ

LYQEQIRVLGADLPPLTYENLQKLELQTNVIKETLRLHAPIHSMLRTVKYPLQVEGTSYVIPNSHNVLSSPGFTA*SPE

YFSKPLDWEPHRWNGVNATG

DDEQVDYGYGLVSKGAGSPYLPSGAGRHRCIGEQFAYVQLCAICVALVRELKFKKLPNVEDVPATNYSSLFSRP

WANSVVQYERRDAAAKD

 

>CYP51F1  Neosartorya fischeri NRRL 181

         XM_001261294.1, NFIA_024690

         98% to CYP51F1 A. fumigatus = ortholog

         63% to CYP51F2 A. fumigatus

MGLIAFILDGICKHCSTQSTWVLVGVGLLSILAVSVIANVLQQL

LFKNPHEPPVVFHWFPFIGSTISYGIDPYKFFFDCRAKYGDIFTFILLGKKTTVYLGT

KGNDFILNGKLRDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFVKYGLTSDALR

SYVPLITDEVESFVKNSPAFQGHKGVFDVCKTIAEITIYTASRSLQGKEVRSKFDSTF

AELYHNLDMGFAPINFMLPWAPLPHNRKRDAAQRKLTETYMEIIKARRQTGSKKDSED

MVWNLMSCVYKNGTPVPDEEIAHMMIALLMAGQHSSSSTASWIVLRLATRPDIMEELY

QEQIRVLGSDLPPLTFDNLQKLDLHAKVIKETLRLHAPIHSIIRAVKNPMAVDGTSYV

IPTSHNVLSSPGVTARSEEHFPNPLEWDPHRWDEDIAASAEDDEKVDYGYGLVNKGTN

SPYLPFGAGRHRCIGEQFAYLQLGTITAVLVRLFKFRNLPGVDGVPDTDYSSLFSKPL

GRSFVEFEKRESATKA

 

>CYP51F1  Ajellomyces capsulatus NAm1

         XM_001540158.1

         63% to CYP51F2 A. fumigatus

         74% to CYP51F1 A. fumigatus

MGLLADVVARVCAHCSTLSPWAFLLTGSATLIVLSVIINVLHQL

LWKNPNEPPVVFHWFPIIGSTISYGIDPYKFFLNCREKYGDIFTFVLLGKKTTVFLGT

KGNDFILNGKLKDVCAEEVYSPLTTPVFGSHVVYDCPNSKLMEQKKFVKYGLTSESLR

SYVTLITDEFNQYIKTSPAFQGDKGVLDVCKSIAEITIYTASRSLQGKEVRSKFDSSF

AQLYHDLDMGFTPINFMLPWAPLPHNRKRDAAQQKMTKIYTDIIRQRREAGGKKDSED

MVWNLMSCVYKDGTPLPDIEIAHMMIALLMAGQHSSSSTFSWIILRLASCPHIIEELY

EEQKQVLGEDLPPLTYETLQKLNLNSHVIRETLRIHAPIHSILRAVKSPMPVDGTRYT

IPTTHNLLAAPGVTSRLPEHFPNPMTWDPHRWENPAMAPEGDQSDEKLDYGYGLVSKG

ANSPYLPFGSGRHRCIGEQFAYVQLGTLLVAIVRQLKLKKLDGETGVPATDYSSLFSK

PLGKPLVAWERRNPTQK

 

>CYP51F1 Histoplasma capsulatum G217B, teleomorph: Ajellomyces capsulatus

96% to CYP51F1    Ajellomyces capsulatus NAm1

HCB02684.1 model is a fushion protein, top part deleted

MGLLADVVARVCAHCSTLSLWALLLAGSASFIVLSVIINVLHQLLWKNPNEPPVVFHWFPIIGSTISYGIDPYKFFLNCR

EKYGDIFTFVLLGKKTTVFLGTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNSKLMEQKKFVKYGLTSESLR

SYVTLITDEFNQYIKTSPAFQGDKGVLDVCKSISEITIYTASRSLQGKEVRSKFDSSFAQLYHDLDMGFSPINFMLPWAP

LPHNRKRDAAQQKMTKIYTDIIRQRREAGGKKDSEDMVWNLMSCVYKDGTPLPDVEIAHMMIALLMAGQHSSSSTLSWII

LRLASCPHIIEELYEEQKQVLGEDLPPLTYETLQKLNLNSHVIRETLRIHAPIHSILRAVKSPMPVDGTRYTIPTTHNLL

AAPGVTSRLPEHFPNPMTWDPHRWENPAMAQEEDQSGEKLDYGYGLVSKGANSPYLPFGSGRHRCIGEQFAYVQLGTLLA

AIVRQLKLKKLDGETGVPATDYSPLGKPLVAWERRNPTQK

 

>CYP51F1  Venturia inaequalis strain Ent27

         GenEMBL AF227920

         14 alpha-demethylase

MGLLSPLLAXLPGSDRSWLFYTLASFGFTVAIVAANLVKQLLFS

NPNEPPVVFHWFPFFGNTVVYGIDPIKFFAECKEKHGDIFTFILLGRKTTVYIGTKGN

EFILNGKQSHVNAEEIYSPLTTPVFGSDVVYDCPNSKLMEQKKFVKYGLTTE ALKSYV

TLIQQEVEDYTKRYPQFKGEKGSFDVCASMAEITIFTASRSLQGKEVRDKFDASFADL

FHDLDMGFSPINFMLPWAPLPHNRRRDAANKKMTETYLEIIQSRKAEGVKKDSEDMIW

NLMQCVYKNGTPIPDKEIAHMMIALLMAGQHSSSSTSSWILLRLATRPDIQEELYQEQ

IRVCGADLPPLQYEDLARMPLHNQIIKETLRMHSPIHSILRAVKQPMPVEGTPYTIPT

SHVLLAAPIASGGSPMYFPAPEKWEPHRWDEGSGGTNISGGENGGEEKEDYGYGLITK

GASSPYLPFGAGRHRCIGEQFAYMQLNTVLATQVREFKFSLREGESFPKTDFSSLFSG

PLRPAWLNWERREKSS

 

>CYP51F1 Issatchenkia orientalis S75391

MPWVGSAVVYGMQPYEFFENCRKQHGDVFSFLLLGKVMTVYLGP

KGHEFVLNAKLSDVSAEDAYTHLTTPVFGKGVIYDCPNWKLMEQKKFAKVALTKESFI

RYVPLIKDEMLKYFNANFRGDSGKTDVLKSQSEMTLFTASRSLFGDALRNRLDASYAE

MYSDLDKGFTPLNFVFSYLPLPNYWKRDAAHKNISNTYLDLINTKRAGGEIKNEDLVD

ALLKNSVYKDGTRMTDEELAHLMIGVLMGGQHTSSATSAWFLLHLGEKPQLQEEIYRE

IQSVLGENFERELTYDDLQKLDLVNATIKETLRLHMPLHSIFRKVTRDLPVPNTSYIV

PKGHYVLISPGYTMLSERYFPNASEFQPHRWDEIKSIDGGISFGAEGENAKETVDYGF

GKISKGVASPYLPFGGGRHRCT

 

>CYP51F1 Uncinula necator U72657

MYIADILSDLLTQQTTRYGWIFMVTSIAFSIILLAVGLNVLSQL

LFRRPYEPPVVFHWFPIIGSTISYGIDPYKFYFDCRAKYGDIFTFILLGKKVTVYLGL

QGNNFILNGKLKDVNAEEIYTNLTTPVFGRDVVYDCPNSKLMEQKKFMKTALTIEAFH

SYVTIIQNEVEAYINNCVSFQGESGTVNISKVMAEITIYTASHALQGEEVRENFDSSF

AALYHDLDMGFTPINFTFYWAPLPWNRARDHAQRTVARTYMNIIQARREEKRSGENKH

DIMWELMRSTYKDGTPVPDREIAHMMIALLMAGQHSSSSTSSWIMLWLAARPDIMEEL

YEEQLRIFGSEKPFPPLQYEDLSKLQLHQNVLKEVLRLHAPIHSIMRKVKNPMIVPGT

KYVIPTSHVLISSPGCTSQDATFFPDPLKWDPHRWDIGSGKVLGNDAVDEKYDYGYGL

TSTGASSPYLPFGAGRHRCIGEQFATLQLVTIMATMVRFFRFRNIDGKQGVVKTDYSS

LFSMPLGTPLIGWEKR

 

>CYP51F1 Lodderomyces elongisporus

77% to CYP51F1   Candida parapsilosis

LELG_03738

MALVDLAIQGYTFFMSLTTFQQVFILIVVPFVYNIAWQLLYSLRKDRVPLVFYWIPWFGSAVGYGQQPYEFFEQCRQKYG

DVFAFVLLGKVMTVYLGPKGHEFILNAKLSEVSAEEAYKHLTTPVFGKGVVYDCPNQRLMEQKKFAKFALTTDSFRKYVP

LIREEVLNYFTSNSNFNMKNQKSGVADVMKTQPEITIFTASRSLMGKEMRSRFDESFAELYSDLDKGFTPVNFVFPNLPL

PHYWKRDAAQQKISSTYMKEITKRRQAGAIDGDEDLISSLMVHSTYKDGVKMTDQEIANLLIGILMGGQHTSASTSAWFL

LHLGDKPELQEQLYEEIQQALQSKGGNLSDLSYEDLQKMTLVNNTIKETLRMHMPLHSIFRKVMSPMVVPGTTYVVPKGH

HVLVSPGYAHTNERYYKNANQFDPHRWDNANMSNKDSDEVDYGFGKVSKGVASSYLPFGGGRHRCIGEQFAYVQLGTLLT

TFIYNIKWKLKGGHVPHVDYASMVTLPELPAEIVWEKRDTCVV

 

>CYP51F1 Candida lusitaniae

72% to CYP51F1   Candida tropicalis

CLUG_04932.1

MALAEYVAWGYAQFCSLGLLSKIALLVFTPIVFNVLWQFVYSLRKDRVPMVFHWIPWFGSAIPYGMQPYEFFESCREKYG

DVFAFMLLGRVMTVYLGPKGHEFVLNSKLADVSAEEAYSHLTTPVFGKGVIYDCPNHRLMEQKKFAKFALTKDSFQKYVP

RIQEEILDYFKSCSDFSMTERNSGVANVMKTQPELTILTASKSLMGDDMRARFDTSFAQLYSDLDKGFTPINFVFPHLPL

PAYRRRDEAQRKISATYMSLINDRRKSGDIVPDRDLIDSLMLNSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWFL

LHLAEKPELQEELYNEVLSVLTKKGGKITDLSYEDLQEMHLVNQTIKETLRLHMPLHSIFRKVKHNLVVPNTSYVVPKGH

HVLVSPGYAQTSERWFPKADTFDPHRWDNDWASSVSEGDAVDYGFGKITKGVSSPYLPFGGGRHRCIGEQFAYVQLGTIL

ATYVYNIKWHYAKGHSLPDVDYQSMVTLPMAPAEIVWEKRETCVI

 

>CYP51F1 Candida dublinensis

93% to CYP51F1  Candida albicans, cdub_2-g61.1, GenEMBL AJ012573

MAIVETAIDGINYFLSLSVTQQITILLGVPFVYNLIWQYLYSLRKDRAPLVFYWIPWFGSAASFGQKPYEFFESCRQKHG

DIFSFMLLGKIMTVYLGPKGHEFIFNAKLSDVSAEDAYKHLTTPVFGKGVIYDCPNSRLMEQKKFAKFALTTESFKRYVP

KIREEILDYFVNDESFKLKEKNHGVANIMKTQPEITIFTASRSLFGDDVRKLFDRSFAQLYSDLDKGFTPINFVFPNLPL

PHYWRRDAAQKKISATYMKEIKLRRERGDIDPSRDLIDSLLTHSTYKDGVKMTDQEIANLLIGILMGGQHTSASTSAWFL

LHLGEKPHLQDAIYQEVVELLKEKGGDLNDLTYEDLQKLPSVTNTIKETLRMHMPLHSIFRKVKNPLRIPETNYVVPRGH

YVLVSPGYAHTSERYFDNPNNFDPTRWDTAAAKANSVSFNSSDEVDYGFGKISKGVSSPYLPFGGGRHRCIGEQFAYVQL

GTILTTFVYNLRWTVEGNRVPDPDYSSMVVLPTEPARIIWEKRETCMF

 

>CYP51F1 Candida glabrata S75389

CAG58795.1

MSTENTSLVVELLEYVKLGLSYFQALPLAQRVSIMVALPFVYTITWQLLYSLRKDRPPL

VFYWIPWVGSAIPYGTKPYEFFEDCQKKYGDIFSFMLLGRIMTV

YLGPKGHEFIFNAKLADVSAEAAYSHLTTPVFGKGVIYDCPNHRLMEQKKFVKGALTK

EAFVRYVPLIAEEIYKYFRNSKNFKINENNSGIVDVMVSQPEMTIFTASRSLLGKEMR

DKLDTDFAYLYSDLDKGFTPINFVFPNLPLEHYRKRDHAQQAISGTYMSLIKERREKN

DIQNRDLIDELMKNSTYKDGTKMTDQEIANLLIGVLMGGQHTSAATSAWCLLHLAERP

DVQEELYQEQMRVLNNDTKELTYDDLQNMPLLNQMIKETLRLHHPLHSLFRKVMRDVA

IPNTSYVVPRDYHVLVSPGYTHLQEEFFPKPNEFNIHRWDGDAASSSAAGGDEVDYGF

GAISKGVSSPYLPFGGGRHRCT

 

>CYP51F1 Candida parapsilosis

75% to CYP51F1   Candida tropicalis

AF019902, CPAG_03310

MALVDLALHGYNYFMTLSTLQQFGLLVFAPFIYNIIWQLLYSLRKDRVPLVFYWIPWVGSAVSYGQDPYGFFEQCREKYG

DLFSFVMLGRVMTVYLGPKGHEFVFNAKLSDVSAEDAYQHLTTPVFGKGVIFDCPNARLMEQKKFAKTALTTDSFRRYVP

LIRGEILDYFTKSKVFNMKKQKSGVVDVLQSQPEITIFTASRSLLGEAMRKRFDASFAQLYADLDKGFTPINFVFPHLPL

PHYWKRDAAQQKISETYMTEIARRRETGDIDENRDLIDSLLVNSTYKDGVKMTDQEIANLLIGVLMGGQHTSATTSAWFL

LHLAEKPQLQDELYQEVLNALSGKGGNLDDLSYEDLQQMPLVNNTIKETLRLHMPLHSIFRKVVSPLVVPNTKYIVPRGH

HVLVSPGYAHTNERFYKDASDFNPHRWDESASTNDAGEVDYGFGKVSKGVSSSYLPFGGGRHRCIGEQFAYVQLGTILTT

FVYNLKWKLANGKVPDVDYTSMVTLPQHPAEIVWEKRDTCVI

 

>CYP51F1 Candida albicans X13296, orf19_922

MAIVETVIDGINYFLSLSVTQQISILLGVPFVYNLVWQYLYSLR

KDRAPLVFYWIPWFGSAASYGQQPYEFFESCRQKYGDVFSFMLLGKIMTVYLGPKGHE

FVFNAKLSDVSAEDAYKHLTTPVFGKGVIYDCPNSRLMEQKKFAKFALTTDSFKRYVP

KIREEILNYFVTDESFKLKEKTHGVANVMKTQPEITIFTASRSLFGDEMRRIFDRSFA

QLYSDLDKGFTPINFVFPNLPLPHYWRRDAAQKKISATYMKEIKSRRERGDIDPNRDL

IDSLLIHSTYKDGVKMTDQEIANLLIGILMGGQHTSASTSAWFLLHLGEKPHLQDVIY

QEVVELLKEKGGDLNDLTYEDLQKLPSVNNTIKETLRMHMPLHSIFRKVTNPLRIPET

NYIVPKGHYVLVSPGYAHTSERYFDNPEDFDPTRWDTAAAKANSVSFNSSDEVDYGFG

KVSKGVSSPYLPFGGGRHRCIGEQFAYVQLGTILTTFVYNLRWTIDGYKVPDPDYSSM

VVLPTEPAEIIWEKRETCMF

 

>CYP51F frag. Candida guilliermondii X97680

this seq is 94% to Candida albicans but only 66% to

full length CYP51A1 for C. guilliermondii

This is a different gene

MTDQEIANLLIVILMGGQHTS--TSAWFLLHLGEKPHLQDVIYQEV

VELLKEKGGDLNDLTYEDLQKLPSVNNTIKETLRMHMPLHSTFRKVTNPLRSPNKYMS

SHTVYMFKFL

 

>CYP51F1 Candida guilliermondii

71% to CYP51F1   Candida lusitaniae

PGUG_03415.1

MAIADIATWVYSEFRGLPVSAQISIVVLFPFVYNLLCQFWYSLQKDRAPLVFHWVPWLGSSVVYSMQPYEFFESCRQKYG

DVFSFVMVGRVMTVYLGPKGHEFVFNSKATDVSAEDAYTHLTTPVFGKGVIYDCPNSRLMEQKKFAKFALTKDSFIKYVP

KIQDEVLSYFVEHDFKMKEKDSGVVNVMKTQPEITIFTASRSLMGDEMRKRFDTSFAQLYSDLDKGFHPIHFAFPHLPLP

MSRNRDRAQQKISATYKGLIDTRRSSGNIDPTRDLIDSLMATSTYKDGVKMTDQEIANLLIGILMGGQHTSAATSAWFLL

HLGERPDLQEEMYKEICGVFEKKNKSFKDLSYDDLQEMPLVNNVIKETLRMHMPLHSIFRKVKKPLLVPGTKYTVPKGHY

VMVSPGYAMVNDRWFPDAASFNPRRWDEQPLPTDGQDETVDYGFGNISKGVSSPYLPFGGGRHRCIGEQFAYVQLGTILA

SYVYNVTWELKDKLPGVDYASMVTLPLEPADIVWKKRRTCVF

 

>CYP51F1 S. pombe Z54096

MAFSLVSILLSIALAWYVGYIINQLTSRNSKRPPIVFHWIPFVG

SAVAYGMDPYVFFRECRAKYGDVFTFVCMGRKMTAFLGVQGNDFLFNGKLADLNAEEA

YSHLTTPVFGKDVVYDIPNHVFMEHKKFIKSGLGFSQFRSYVPLILNEMDAFLSTSPD

FGPGKEGVADLLKTMPVMTIYTASRTLQGAEVRKGFDAGFADLYHDLDQGFSPVNFVF

PWLPLPRNRRRDRAHKIMQKTYLKIIKDRRSSTENPGTDMIWTLMSCKYRDGRPLKEH

EIAGMMIALLMAGQHTSAATIVWVLALLGSKPEIIEMLWEEQKRVVGENLELKFDQYK

DMPLLNYVIQETLRLHPPIHSHMRKVKRDLPVPGSKIVIPANNYLLAAPGLTATEEEY

FTHATDFDPKRWNDRVNEDENAEQIDYGYGLVTKGAASPYLPFGAGRHRCIGEQFAYM

HLSTIISKFVHDYTWTLIGKVPNVDYSSMVALPLGPVKIAWKRRN

 

>CYP51F1 Schizosachharomyces japonicus

78% to Schizosachharomyces pombe CYP51A1  SJAG_03750

MVVSMVSILLSVGLAWYVGYIANQIFNRDPKRPPVVFHWFPFIGSAVTYGIDPYVFFRQCRAKYGDVFTFVLMGRKITAY

LGVKGNDFLFNGKLADLSAEEAYSHLTTPVFGKDVVYDVPNHVLMEHKKFIKSGLGFDQFRAYVPLIVNEMHDFLAKAPT

WGPGKSGVADLLATMPVMTIYTASRTLQGEEVRAGFNAGFADLYHDLDQGFTPINFIFPWLPLPRNKRRDRAHNIMAETY

LNIIKKRRAAHENPGTDMIWTLMGSRYRDGRPLKDHEIANMMIALLMAGQHTSAATIMWILSLLGSKPEVIEMLYEEQKR

VFGKELRPLDFDSYKDLHLVNYVIREALRLHPPIHSIMRKVKRNLAVPGTKIVIPAGHHLLAAPGVTATDEEFFAHAKDF

DPERWNSRVNEDEGDVERVDYGYGLVTKGAASPFLPFGAGRHRCIGEQFATMHLCTLVSTFVREYKWTTVGGVPKVDYSS

MVALPLGPVKIAWERRE

 

>CYP51F1 Schizosachharomyces octosporus

SOCG_01956

MAISLLPLVLSGILAWYVGYILNQITNRNPKRPPIVFHWVPFFGSAIPYGIDPYAFFHECREKYGDVFTFVCMGRKMTAY

LGVKGNDFLFNGKLADLSAEEAYSHLTTPVFGKDVVYDVPNHVLMEHKKFIKSGLGFPQFRSYVPLILNEMDAFLNTSPE

FGPGKEGIADLLKTMPVMTIFTASRTLQGAEVREGFDAGFADLYHDLDQGFSPINFVFPWLPLPRNRRRDRAHNIMQQTY

LNIIKQRRSSPENPGSDMIWTLMNSKYRDGRNLKEHEIAGIMIALLMAGQHTSAATIMWALTLLGSKPEVIEMLYEEQKR

VLGEGENFHVDFDKYKDLPLLNYVIRETLRLHPPIHSIMRKVKRDLPVPDSKMVIPAGSHLLAAPGYSATHEDFFTHAKE

FCPERWGERPDDEENAEQIDYGYGLVSKGGSSPYLPFGAGRHRCIGEQFAYMHLTTIICKFVHDYTWTTVGKVPDVDYSS

MVALPLAPVKIAWKRRD

 

>CYP51F1 Pneumocystis carinii

AY228706 58% to CYP51F1 S. pombe

contig_349-snap.1 from Fungal Genome DB

contig_349-snap.2 from Fungal Genome DB

MGFLKTILKVLGIENFTLSFVIIILTFFFLLHIFPQLYQKDSSK

PPVVFHWLPFIGSTIQYGMDPYKFFQKQKKKHGNIFTFILLGKKMTVALGPKGNDILF

NGKLSSLSAEEAYTHLTTPVFGTDVVYDVPNHVLMEQKKFVKTGFTIETFRAYVPLII

EEVKTYLETSPIFGKDKLSGVSSLMKALPEITIFTASRTLQGKEVRSNFDASFAKLYH

DLDGGFTPINFLAPWLPLPKNRLRDAAQKKMAQIYMNIIKQRRKTCQHEEKDMIWNLM

NQHYKDGRKLTDKEIAHLMIAILMAGQHTSAATGCWALLHLAEKPEYIKLLLEEQKRV

FGDNLDDLTYDNLKDMELLSYVIKETLRLHPPLHSIIRKVKSPILIENSPYIVPKNHY

LLAAPGVSSVDEEYFENALEFIPERWKCEKNTEDSDKIDYGYGLVTKGAFSPYLPFGA

GRHRCIGEQFAYMQLGTIITIFVHELEWTLPKNQITIPKPDYTSMVVLPERPSNIEWR

RRQKR

 

>CYP51F1 Yarrowia lipolytica

63% to CYP51F1   S. cerevisiae

CAG82748.1

MIILTTLNNMGIPVEGWHILAGLAILAVITEIYVIGSQLLARRNKTLAPMAFYWIPWVGSSIPYGIDPYEFFEDCRNRYG

DVFSFYMLGRVMTVSLGTKGHEFVFNSKLADVSAEEAYTHLTTPVFGTGVIYDCPNSRLMEQKKFCKGALTRDAFRSYVP

KIVEEVTNFFAVNFEGKTGKADVMTTQPQMTIFTASRCLLGDEIRSKLNGDFAKLYSDLDNGFTPINFVFPNLPLPSYRK

RDLAQQKIRDTYMSVIQRRRMEKDVQDRDLIDALLKNHTYKDGKRMTPQEIAHLLIGVLMGGQHTSASTSAWMLLRLGLD

PAIQDELYQEQVDILGEADGSFRQPTYEDILTMTKLQNTIKETLRLHMPIHSIFRQVMRDLPVPGTSFVVPKGHFVMASP

GYSQQAERYFPNAKKFDPRRWMTPTEKMAESETDAEAGETVDYGFGAISKGVSSPYLPFGGGRHRCIGEQFANCQLTTLM

SCYIQNFKWTVPEGSKLPAVDTTSMITLPVHPSYIVWSKRERN

 

>CYP51F1 S. cerevisiae yeast M15663 U10555

MSATKSIVGEALEYVNIGLSHFLALPLAQRISLIIIIPFIYNIV

WQLLYSLRKDRPPLVFYWIPWVGSAVVYGMKPYEFFEECQKKYGDIFSFVLLGRVMTV

YLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALTK

EAFKSYVPLIAEEVYKYFRDSKNFRLNERTTGTIDVMVTQPEMTIFTASRSLLGKEMR

AKLDTDFAYLYSDLDKGFTPINFVFPNLPLEHYRKRDHAQKAISGTYMSLIKERRKNN

DIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSAATSAWILLHLAERP

DVQQELYEEQMRVLDGGKKELTYDLLQEMPLLNQTIKETLRMHHPLHSLFRKVMKDMH

VPNTSYVIPAGYHVLVSPGYTHLRDEYFPNAHQFNIHRWNNDSASSYSVGEEVDYGFG

AISKGVSSPYLPFGGGRHRCIGEHFAYCQLGVLMSIFIRTLKWHYPEGKTVPPPDFTS

MVTLPTGPAKIIWEKRNPEQKI

 

>CYP51F1 Saccharomyces paradoxus

98% to 51F1 S. cerevisiae Spar_10379

MSATKSIVGEALEYVNIGLSHFLALPLAQRISLIIITPFIYNIVWQLLYSLRKDRPPLVFYWIPWVGSAVVYGMKPYEFF

EECQKKYGDIFSFVLLGRVMTVYLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALTK

EAFKSYVPLIAEEVYKYFRDSKNFRLNERSTGTIDVMVTQPEMTIFTASRSLLGKEMRQKLDTDFAYLYSDLDKGFTPIN

FVFPNLPLEHYRKRDHAQKAISGTYMSLIKERRKNNDIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSA

ATSAWILLHLAERPDVQQELYEEQMRVLDGGKKELTYDLLEEMPLLNQTIKETLRMHHPLHSLFRKVMKDMHVPNTSYVI

PAGYHVLVSPGYTHLRDEYFPHAHQFNIHRWNNDSASSYSVGEEVDYGFGAISKGVSSPYLPFGGGRHRCIGEHFAYCQL

GVLMSIFIRTLKWHYPEGKSVPPPDFTSMVTLPTGPAKIIWEKRNPEQKV

 

>CYP51F1 Saccharomyces mikatae

96% to CYP51F1   S. cerevisiae Smik_9639

MSATKSIVGEALEYVNIGLSYFLALPLAQRISLIIITPFIYNIVWQLLYSLRKDRPPLVFYWIPWVGSAIAYGMKPYEFF

EECQKKYGDIFSFVLLGRVMTVYLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALSK

EAFKSYVPLIAEEVYKYFRDSKNFRLNERSTGTIDVMVTQPEMTIFTASRSLLGKEMRQKLDTDFAYLYSDLDKGFTPIN

FVFPNLPLEHYRKRDHAQKAISGTYMSLIKERRKNNDIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSA

ATSAWILLHLAERPDVQQELYEEQMRVLDGGKKELTYDLLEEMPLLNQAIKETLRMHHPLHSLFRKVMKDMHVPNTSYVI

PEGYHVLVSPGYTHLRDEYFPHAHQFNIHRWNNDAASSYSVGEEIDYGFGAISKGVSSPYLPFGGGRHRCIGEHFAYCQL

GVLMSIFVRTLKWHFPEGKTVPSPDFTSMVTLPTGPAKIIWEKRDPEQKI

 

>CYP51F1 Saccharomyces kudriavzevii

96% to CYP51F1   S. cerevisiae Skud_Contig2067.14

MSASKSIVGEALEYVSIGLSYFLALPLAQRISLIIIAPFIYNIVWQLLYSLRKDRPPLVFYWIPWVGSAIPYGTKPYGFF

EQCQKKYGDIFSFVLLGRVMTVYLGPKGHEFVFNAKLADVSTEAAYSHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALSK

EAFKSYVPLIAEEVYKYFRDSKNFRLNERSTGTIDVMVTQPEMTIFTASRSLLGKEMREKLNTDFAYLYSDLDKGFTPIN

FVFPNLPLEHYRKRDHAQKTISGTYMSLIKERRKNNDIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSA

ATSAWILLHLAERPDVQQELYDEQMRVLDGGKKELTYDLLEEMPLLNQTIKETLRMHHPLHSLFRKVMKDMHVPNTSYVI

PAGYHVLVSPGYTHLRDEYFPQAHQFNIHRWNNDAASSYSVGEEIDYGFGAISKGVSSPYLPFGGGRHRCIGEHFAYCQL

GVLMSIFVRTLKWHFPEGKSVPPPDFASMVTLPTGPAKVIWEKRNPEQKI

 

>CYP51F1 Saccharomyces bayanus

93% to CYP51F1 S. cerevisiae Sbay_23211

MSATKSIVAEALEYVNIGLSHFMGLPLAQRISLVIIAPFIYNIVWQLLYSLRKDRPPLVFYWIPWVGSAVTYGMKPYEFF

EQCQKKYGDIFSFVLLGRVMTVYLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNSRLMEQKKFVKGALSK

EAFKSYVPLIAEEVYKYFRDSKNFRMNEKSSGTIDVMITQPEMTIFTASRSLLGKEMRQKLDTDFAYLFSDLDKGFTPIN

FVFPNLPLEHYRKRDHAQRAISGTYMSLIKERRKNNDIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTSA

ATSAWILLHLAERPDVQEELYEEQMRVLDGGKKELTYDLLEQMPFLNQTIKETLRLHHPLHSLFRKVMKDMHVPNSSYVI

PEGYHVLVSPGYTHLRDEYFPHAHEFNIHRWNNDASSSYSVGEEVDYGFGAISKGVTSPYLPFGGGRHRCIGEHFAYCQL

GVLMSIFVRTMKWHFPEGKSVPSPDFTSMVTLPIGPAKIIWEKRNPEQKI

 

>CYP51F1 Saccharomyces castellii

78% to CYP51F1 S. cerevisiae Scas_Contig699.20

MSDSITEAGNSSLIGQVFQYLQLAFQIFQSLPLAQRISLIVLAPFLYSITWQFIYSFRKDRPPLVFYWIPWVGSAVPYGH

KPYVFFENCQKKYGNIFSFMLLGRIMTVYLGPKGHEFIFNAKLADVSAEDAYSHLTTPVFGKGVIYDCSNSRLMDQKKFV

KGALSKEAFKRYVPLFKEEILKYFKDSNNFKFNQREKGEIDVMVTQPEMTIFTASRTLLGKEMRDKLDTDFAYLYSDLDK

GFTPINFVFPNLPFLEHYKKRDNAQRTIASTYLSLIKKRRANNDIQDRDLIDTLMKNSTYKDGVKMTDPEIANLLIGVLM

GGQHTSAATSAWCLLHLAERPDVQEELYQEQMRVLDNGKKEITYDLLEQMPLLNQMIKETLRLHHPLHSLFRKVIRDMQV

PNTSYVIPKDYFVLVSPGYTHLQEHYFPQAHSFNIHRWDNDAESSYVVGEEVDYGFGAITKGVTSPYLPFGGGRHRCIGE

HYAYCQLGVIMAEFIREMKWHFPSKDQTVPVSDFTSMVTLPTGPAKIVWEKRVPHETV

 

>CYP51F1  Saccharomyces kluyveri

79% to CYP51F1 S. cerevisiae  Sklu_Contig2436.11

MSSNKVSIVQTAFEYVQLGFSHFLNLSLAQQISLVVLFPFFYSIVWQLLYSLRKDRVPMVFYWIPWVGSAVGYGTRPYEF

FAECQKKYGDVFSFMLLGRVMTVCLGPKGHEFVLNAKLADVSAEAAYTHLTTPVFGKGVIYDCPNNRLMDQKKFAKGSLT

RDSFKVYVPKIIDETYKYFRNSKNFLLNQNKSGVANVMETQPEMTIFTASRSLLGEEMRAKLDTDFAYLYSDLDKGFTPI

NFVFSHLPLESYRKRDRAQQLISSTYMSLIRKRRENNDIQDRDLIDALMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTS

AATSAWLLLHLAEKPELQEELYAEQMRVLDNGKKELTYDLLQEMPLLNQTIKETLRMHHPLHSIFRKVMRDLPVPNTSYV

VPKGHHVLVSPGYAHLRDEYFPHAATFDPHRWDNDAASSYSSSEQVDYGFGSVSKGVSSPYLPFGGGRHRCVGEHFAYCQ

LGTIMSVYIRTLKWSFPPEMKGVPSPDFQSMVTLPCEPAKIKWEKREPAQTI

 

>CYP51F1  Kluyveromyces waltii

77% to CYP51F1 S. cerevisiae kwal_154-g13.1

MDSTNSSVVGKIFSKGIEIGSALLSRFLALSIAQQASLIIAAPFVYVIVWQFLYSMRKDRVPLAFYWIPWVGSAVTYGMR

PYEFFAECQEKYGDVFSFVLLGRVMTVCLGPKGHEFVLNAKLADVSAEAAYTHLTTPVFGKGVIYDCPNARLMEQKKFVK

GALSTESFRGYVPLIAEEVYQYFRNSKNFDMNHKKSGVANVMVTQPEMTIFTASRSLLGKEMRNLLNTDFAYLYSDLDKG

FQPINFVFSHLPLEHYRKRDHAQQTISSTYMKLIKQRRENNDIQDRDLIDTLMKNSTYKDGTKMTDQEIANLLIGVLMGG

QHTSAATSAWMLLHLAERPDLQEELYAEQMEVLDNGKKELTYELLQQMPLLNQTIKETLRMHHPLHSIFRRVERDLPVPK

TQYVVPRGHFVLVSPGYCHLQDEYFPKANTFDPHRWNNDYKSSYATGEEVDYGFGAISKGVSSPYLPFGGGRHRCIGEHF

AYCQLGVLMSVYIRTVKWKFTPKMHGVPKPDFASMVTLPLCPAEIEWEKRDPQQAL

 

>CYP51F1 Ashbya gossypii ATCC 10895

71% to CYP51F1 S. cerevisiae, NP_984259.1

MSESLLQTVVAYVELVLHHFMALSWTQQLSIVIVAPFIYSLVWQTLYSFRKDRVPLVPFMVPWVGSALAYGRAPYEFFGK

CQQKYGDVFAFMLLGRVMTVYLGTKGHEFILNAKLAEVSAEEAYTKLTTPVFGEGVVYDCPNHRLMEQKKFCKNALSTEA

FRRYVPMVMDEVRKYLRTSKHFMMNERSSGVVNVMETQPEMTIFTASRSLLGAEMHSMLDADFAYLYADLDKGFTPLNFV

FRDLPLDNYRRRDNAQRTISSTYMKVIERRRKNNDVQDRDLIDALMTSAQYKDGVKMTDQQIANLLIGVLMGGQHTSAAT

SAWVLLHLAERPDIQEELYEEQMRVLDGGAKELTYELLQEMPLLNQVIKETLRMHHPLHSLFRKVTRDMPVPNTSYVIPK

DHYVLASPGFCHLSEEYFPNAKEFNPHRWDNDAASSVSTGEKVDYGFGAISKGVSSPYLPFGGGRHRCIGEGFAYMQLGT

IFSVVVRSMKWHFPADMKGVPNPDFTSMVTLPSEPCRIAWERRVPDQII

 

>CYP51F1 Kluyveromyces polysporus

Kpol_1004p61

81% to CYP51F1 S. cerevisiae

MSDQSIVASLAEYVTIGLSYFMSLPLSQRISIVFIAPFIYSITWQLLYSLRKDRPPLVFYWIPWVGSAVTYGSKPYQFFG

DCQKKYGDCFSFMLLGRIMTVYLGPKGHEFVFNAKLADVSAEAAYAHLTTPVFGKGVIYDCPNHRLMDQKKFVKGALTKE

AFARYVPLMVEEMVKYLRSSPFFKINEKSEGTVNVLATQSEMTIFTASRSLLGKEMRDKLDTDFAYLYSDLDKGFTPINF

VFPNLPLEHYRKRDNAQRVISSTYLSLIKKRRDENDIQDRDLVDTLMKNSTYKDGVKMTDKEIANLLIGVLMGGQHTSAA

TSAWALLHLAERPDVQKELYEEQMRVLDNGKKELTYDLIQEMPLLNQCIKETLRLHHPLHSIFRKVMKDMKVPNTSYVVP

KDYYVMVSPGYTHLQDEYFPNAKQFNMHRWDNDATSSYSVGEEIDYGFGVVTKGVSSPYLPFGGGRHRCIGEHFAYCQLG

VLMSIFIRTMTWKFPEGRTVPNSDFQSMVTLPLNPADIIWTKRDAAQVI

 

>CYP51F1 Kluyveromyces lactis

76% to CYP51F1 Saccharomyces kluyveri

KLLA0E03553g

MSTKSESVVVKVFEAAQAVLGQFLAFSIAQQISIVILLPFVYTIVWQLLYSMRKDRVPLVFYWIPWVGSAVSYGMRPYEF

FEECREKYGDIFAFVLLGKVMTVYLGPKGHEFILNAKLADVSAEAAYTHLTTPVFGNGVIYDCPNHRLMDQKKFVKGALT

TDAFRKYVPLIAEEVQKYFLTSKNFSIGEKDHGKINVMVTQPEMTIFTASRSLLGSEMREKLNTDFAYLYSDLDKGFTPL

NFVFSHLPLDNYRKRDNAQRVISSTYLSLIKKRRDTNDIQDRDLIDSLMKNSTYKDGVKMTDQEIANLLIGVLMGGQHTS

AATSAWVILHLAERPDIQEKLYEEQMKVLDNGTKELTFDLLQEMPLLNQTIKETLRLHHPLHSLFRKVMNDMPVPNSSYV

VPKGHHVLVSPGYCHLQDKYFPNASEFNPNRWDNDAASSYASNEKVDYGFGSISKGVSSPYLPFGGGRHRCIGEHFAYMQ

LGTILSNYIRTLTWKFADPSATVPTPDFQSMVTLPLEPSEIEWTLRK

 

>CYP51F1  Kluyveromyces marxianus X97682

88% to CYP51F1   Kluyveromyces lactis

MTDQEIANLLSILMGGQHTSAATSAWAILHLAERPDVQQELYEE

QMRVLDNGKKELTYDLLQEMPFLNQTIKETLRLHHPLHSLFRKVMNDMPVPNTSYVVP

KGDHVLVSPG

 

>CYP51F1 Fusarium graminearum FG01000.1 AACM01000048 FGcontig1.48_scaffold1

MGLLQELAGHPLAQQFQELPLGQQVGIGFAVFLVLSVVLNVLNQLLFRNP

NEPPMVFHWFPFVGSTITYGMDPPTFFRENRAKHGDVFTFILLGKKTTVA

VGPAGNDFILNGKLKDVCAEEIYTVLTTPVFGKDVVYDCPNAKLMEQKKF

MKIALTTEAFRSYVPIISSEVRDYFKRSPDFKGKSGIADIPKKMAEITIF

TASHALQGSAIRSKFDESLAALYHDLDMGFTPINFMLHWAPLPWNRKRDH

AQRTVAKIYMDTIKERRAKGNNESEHDMMKHLMNSTYKNGIRVPDHEVAH

MMIALLMAGQHSSSSTSSWIMLRLAQYPHIMEELYQEQVKNLGADLPPLT

YEDLAKLPLNQAIVKETLRLHAPIHSIMRAVKSPMPVPGTKYVIPTSHTL

LAAPGVSATDSAFFPNPDEWDPHRWEADSPNFPRMASKGEDEEKIDYGYG

LVSKGSASPYLPFGAGRHRCIGEHFANAQLQTIVAEVVREFKFRNVDGGH

TLIDTDYASLFSRPLEPANIHWERRQ*

 

>CYP51F1 Aspergillus nidulans AN8283.1 51 clan

MGLVSLVLDNVCERCSALSVWALSGLGLLSVIIIAVVLNVLRQILFKNPN

EPPVVFHWFPFIGSTISYGIDPYKFFFNCRAQYGDIFTFVLLGKKTTVYL

GTKGNDFILNGKLKDVCAEEVYSPLTTPVFGRHVVYDCPNAKLMEQKKFV

KYGLTSDALRSYVQLITAEVEDFAQKSSVFQNAKGVFDVSRTIAEITIYT

ASRSLQGKEVRDKFDSTFAELYHDLDMGFAPINFMLPYAPLPHNRKRDAA

QRKMAETYMEIIKERRKSGEKKDSEDMVWNLMSCVYKNGTPLSDEEIAHM

MIALLMAGQHSSSSTLSWILLHLARHPEIVEELYQEQLKVLGSDMHMTYD

DLQKLELHSKIIKETLRIHAPIHSIIRAVKSPMPVPGTSYVIPTSHNVLS

SPGVTARSDEFFPNPLKWDPHRWDSNPIANSTEDEEKIDYGYGLVSKGTN

SPYLPFGAGRHRCIGEQFAYVQLITVTAALVRLFKFDTVSESDKSSVPET

DYSSLFSRPAGKCFVQYEKRNVTTKA*

 

>CYP51F1 Nectria haematococca fgenesh1_pg.scaffold_2000426

89% to CYP51F1 F. gram.

Necha1/scaffold_2:1348428-1350174

89% to Fusarium graminearum  CYP51F1

FG01000.1 AACM01000048 FGcontig1.48_scaffold1

This gene model seems correct DRN 2/4/06

MGLLHEIAGHPLAQQFQELPLGQQVGIGFGAIVVLSVILNILSQILFVNPNEPPMVFHWFPFIGSTVTYG

MDPPRFFKENRAK (0)

FGDVFTFVLLGKKTTVAVGPSGNDFILNGKLKDVCAEEIYTVLTTPVFGKDVVYDCPNAKLMEQKK (0)

FMKIALTTEAFRSYVPIISGEVRDYFKKSADFKGKTGIVDIPKKMAEITIFTASHALQGSV

IRSKFDESLAALYHDLDMGFTPINFMLHWAPLPWNRKRDHAQRTVAKIYMDTIRERRIKDNDDSEHDMMK

HLMNSTYKNGTPVPDHEIAHMMIALLMAGQHSSSSTSSWIMLRLAQYPQIMEELYQEQVKALGADLPPLK

FEDLAKLPLNQAIIKETLRLHAPIHSIMRAVKSPMPVPGTKYVIPTSHTLLAAPGVSASDPAYFPNPDEW

DPHRWEAGSPNAPTIARNNAEEEEKIDYGYGLVSKGSASPYLPFGAGRHRCIGEHFANVQLQTIVAEVVR

EFKFSNVDGGNTLIGTDYASLFSRPLEPANIRWERRQ*

 

>CYP51F1 Magnaporthe grisea MG04432.4  75% to CYP51 AACU01001110 cont2.837

MGLLQDTTGPLVDAFYQLGTGAQVGVAFVSFIFLSVFFHVAQQIFFKNPH

EPPVVFSWFPVVGSTVTYGKDPPQFFRDMAKKYGNIFTFILLGKKTTVYI

GTEGNEFILNGKLRDVNAEEIYGPMTTPVFGKDVVYDCPNAKLMEQKKFM

KIALTTEAFRSYVPIIADEVSSYLKRTPAFKGPSGVVNIPPKMAEITIFT

ASHALQGKEIRDQFDETLADLYHDLDMGFHPVNFKLHWLPLPRNIRRDKA

QKTIAKIYMDTIQRRRAKGKDSEAKDMMYHLMNSTYKNGTPVPDHEIAHM

MIALLMAGQHSSSSTSSWIMLRLASRPDIMEELYQEQVRALGADLPPLRY

EDLANLPLHLAVIKETLRLHAPINSILRAVKQDLPVPGTNYVIAKDTTVL

AAPGYSAGDPNHFPEPELWEPHRWEADSRLAPRISMSNDNDEEEKIDYGY

GLVSKGTTSPYLPFGAGRHRCIGEHFANVQLQTIVAMIVREFKFRNVDGS

GKVVGTNYASLFSRPEEPAKIYWERR*

 

>CYP51F1 Ustilago maydis Z48164

MVASSSSATASLLDQLFALTPLADSSAWIKTITVLVLLPLLAVV

LNVASQLLLATPKNHPPVVFHFVPVIGSAIYYGIDPYKFFFECREKYGDVFTFVLLGR

KITVALGPKGSNLVFNAKHQQVTAEDAYTHLTTPVFGKEVVYDVPNAVFMEQKKFVKV

GLSIENFRVYVPQIVDEVREYIKSDARFSALKTRKTITVDIFQAMSELIILTASRTLQ

GKEVRQGLDKSFAQLYHDLDSGFTPINFVIPNLPLPSNFKRDRAQKKMSQFYQDIVAK

RRAAGASTSADDASGENDMIAALIEQKYKNGRALSGVEIAHMMIALLMAGQHTSSATS

SWAFLRLASRPEIIEELYEEQLNVYSDGHGGLRELDYETQKTSVPLLDAVVKETLRLH

PPLHSIMRYVKSDLAVPPTLSSPTSTKSEPDAHYVIPKGHYIMAAPGVSQVDPQIWKS

SDQFDPHRWLDATTAAAMQDSGEDKQDFGFGMISTGANSPYLPFGAGRHRCIGEQFAY

LQIGVILATFVRIFKWHLDSKFPDPDYQSMVVLPSKNGCAIVLTPRAESLHLD

 

>CYP51F1 Puccinia graminis f. sp. tritici

55% to CYP51F1   Malassezia globosa with a short seq gap.

PGTG_07202 revised

MSSLIDPLIEFIGSFSTFNQILIYFLLSITSIISINIFNQLAIPKDPTTPPVVFHLFPFIGSAVSYGIDPYAFLESCRKK

YGNVFTFVLLNKKVTVALGLEGNALILNGKLSQVNAEEAYTAL (1)

TATPVFGTKMVYDVPNAILMXXXXXXXXXXXXX

NFRKYVTLIADETISYLEDHVFE (1)

KPKTQQAVKDCFKVASEITICTAS

ATLQGPEVREGLNKSFANLYHDLDGGFTPLHFAFPNLPLPSYRRRDRAQVAMRNFYMNIIQKRREDNREGQLGDMIDSLQ

GQTYKDGRPLTDKEIAHIMIALLMAGQHTSAATGSWLLLHLASRPDIVAELRQEQIEVFGKPGQTDDKELDPLDLERVQS

PLMLACIKEVLRLHPPIHSIMRKVKSPITVPRTLASHNEDTPYIIPSSNFVLAAPGASQIDPAIWSSPHEFEPSRWLKLT

SPFKAGGGETQEEMVDYGFGMISSGANSPFLPFGAGRHRCIGEQFAYLQLSTLGATVIRNCELELVSNQFPKPDYTTMLV

CPIKPRDVKFTRRNTHS

 

>CYP51F1 Sporobolomyces roseus

55% to CYP51F1   Ustilago maydis, e_gw1.1.480.1

MGLATLALTAVLYFAGASLSIVALNVLHQLLAPRDPTKPPLVFHYVPVIGCAVSYGMDPLGFLDDCKQKYGPVFTYPLLG

RRITATLGPLGSNFVLNGKLAHVNAEEAYTHLTTPVFGTEVVYDVPNHVLMEQKKFVKFGLTTDNFRRYVGLIRQEVLGY

VSTHVFDQDVLANGVDAFTTSSEITILTASATLQGREVRQAMDKSFAELYHDLDGGFTPLNFVFPNLPLPSYRRRDRAQL

KMREFYISILEKRRASTDEPDLDMLTALQNQSYRNGEPLTDKQIAHIMIALLMAGQHTSAATGAWAILRLGENQDLQRRL

WEEQVEYHMDKETNTLNELTYENFQTPLLVATIKELLRMHPPLHSLMRKVISDCPVPSSVGSPSAEPRASHAFKKSNESK

EYVIPKGEFVLAAPGYSQVDESIWGKDAKEFRVERWLESEGGNKVPGDEDEGEEDYGWGKISRGGKSAYLPFGAGRHRCI

GEQFAQVQLGTIIATLVRELTWTLDQPFPGNDYTVRPSLFLLEAHESACTDCAPFV

 

>CYP51F1 Malassezia globosa

67% to CYP51F1   Ustilago maydis, MGL_2415

MLQEIGAWPVWQQALTFLVGGLALIVGINVLVQVLVPRNKSLPPMVFHWVPVVGSAITYGMDPYRFFFNCREKYGDVFTF

KLFGRNVTVALGPKGSNLVFNGRLTQVSAEEAYTSLTTPVFGKGVVYDVPNAVLMEQKRFVKSGLSMENFRMYVTQIESE

VKDFINNDAAFLPLQKGATSVTVDIFNVFSEITILTASRTLQGKEVRESLDKTFAKLYHDLDSGFTPINFVIPNLPLPNN

FRRDRAQRLMSDFYLGIIKKRREGNTEGTEHDMISALMEQSYKNGRNINDREIAHMMIALLMAGQHTSSATGSWAMLRLA

SRPEIIEELYEEQKRVYSDGTGGFAPLDYDIQKSSVPVLDAVIRETLRLHPPIHSIMRKVKSDMVVPPTLAAPISSKGSR

DETYVIPKGHYVIAAPGVSQVDPKIWEDASRFDPHRWLGDKANVMNQTDDAQEDFGWGMVSTGANSPYLPFGAGRHRCIG

EQFAYLQLGTIISTFVRAFDWRLETKLPAPDYTSMVVLPTQPANLVFTPRKNKA

 

>CYP51F1 Penicillium italicum Z49750

MDLVPLVTGQILGIAYYTTGLFLVSIVLNVIKQLIFYNRKEPPV

VFHWIPFIGSTIAYGMDPYQFFFASRAKYGDIFTFILLGKKTTVYLGVEGNEFILNGK

LKDVNAEEVYGKLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLSQEALESYVPLIADET

NAYIKSSPNFKGQSGTIDLAAAMAEITIFTAARTLQGEEVRSKLTSEFADLFHDLDLG

FSPINFMLPWAPLPHNASAIKHTTYARDLSGNYPSATGSWRRRQRRRQDKSKGTDMIS

NLMRCVYRDGTPIPDKEIAHMMITLLMAGQHSSSAISCWILLRLASQPEMAEKLHAEQ

IKNLGADLPPLQYKDMDKLPLLRNVIKETLRLHSSIHTLMRKVKNPMPVPGTDFVVPP

SHTLLSSPGVTARDERHFRDPLRWDPHRWESRVEVEDSSDTVDYGYGAVSKGTRSPYL

PFGAGRHRCIGEKFAYLNLEVIVATLVREFRFFNPEGMEGVPDTDYSSLFSRPVQPAT

VRWEVRS

 

>CYP51F1 Candida tropicalis M23673

 

MAIVDTAIDGINYFLSLSLTQQITILVVFPFIYNIAWQLLYSLR

KDRVPMVFYWIPWFGSAASYGMQPYEFFEKCRLKYGDVFSFMLLGKVMTVYLGPKGHE

FIYNAKLSDVSAEEAYTHLTTPVFGKGVIYDCPNSRLMEQKKFAKFALTTDSFKTYVP

KIREEVLNYFVNDVSFKTKERDHGVASVMKTQPEITIFTASRCLFGDEMRKSFDRSFA

QLYADLDKGFTPINFVFPNLPLPHYWRRDAAQRKISAHYMKEIKRRRESGDIDPKRDL

IDSLLVNSTYKDGVKMTDQEIANLLIGVLMGGQHTSASTSAWFLLHLAEQPQLQDDLY

EELTNLLKEKGGDLNDLTYEDLQKLPLVNNTIKETLRMHMPLHSIFRKVMNPLRVPNT

KYVIPKGHYVLVSAGYAHTSDRWFEHPEHFNPRRWESDDTKASAVSFNSEDTVDYGFG

KISKGVSSPYLPFGGGRHRCIGEQFAYVQLGTILTTYIYNFKWRLNGDKVPDVDYQSM

VTLPLEPAEIVWEKRDTCMV

 

>CYP51F1 N. crassa AABX01000052.1 cont3.136 NCU02624.1 (version3)

2384 MGILQVVAGPLSQQFSQLGTVSQIGVAIASFLFVAVVLNVLQQFLFKKPN

EPPLVFHWFPLIGSTITYGMDPPRFFKENREK (0) 2139

2066 YGDCFTFILLGKKTTVYVGPKGNDFILNGKIRDVNAEEIYTVLT

TPVFGKDVVYDCPNSKLMEQKK (0) 1869

1806 FMKIALTTEAFRQYVPIISDEVTSYLKR

TADFKGKSGIVDIPPKMAQITIFTASHALQGKEIRDKFDETLADLYHDLD

MGFSPINFMLHWAPLPWNNRRDYAQRTVAKIYMDTIKERRARGETGAQDI

MWHLMNSTYKGDVPVPDHEIAHMMIALLMAGQHSSSSTSSWIMLRLASRP

DIMEDLYNEQVKNLGADLPPLTYEDLAKLPLHAAIVKETLRLHAPIHSIM

RAVKTPMPVPGTKYVIPTDHVLLAAPGVSATDESYFPQPDLWEPHRWEKD

SPLAPTIVRNVPSEEDDEKIDYGYGLVSKGANSPYLPFGAGRHRCIGEQF

ANVQLQTILAIIVRNFKFRNVDGSDKVIGTDYASLFSRPLEPAKIYWERR

EGCQL* 655

 

>CYP51F1 ug.2.6.1(CYP51)  Phanerochaete chrysosporium
MSLSQYGPIAGLVGQAYDALASMSTSRLVLFLLINIPILSVVCNVIYQLLPKDKSLPPVVWHWFPWFGSAAAYGEDPIKFFFDCKEKYGNVFTFILMGRKVTVALTPAGNNFIMGGKHTTFSAEEVYGGLTTPVFGKDVVYDCPNELLMEQKKFVKFGLSTENFRQYVGMIEEEVLQFMRNDASFKIYQMNDINEWGAFDVLKVMSEITILTASRTLQGKEVRANITKDYAQVYNDLDGGFTPLHFMFPNLPLESYRKRDAAHKKISDFYISIIRKRRENPGQEEHDMIAALMNQKYRVGRPLKDHEIAHIMIALLMAGQHTSSATGSWALLHIADRPDVAEALYEEQVKHFRQSDGSWRTPEYEELKELPVLDSVIRETLRIHPPIHSIMRAVREDVVVPPTLAAPSEDGRYVIPKGHVVLSSAAISQVDPMLWKNANDWDPSRWSDPEGVAAQAYKQYDDAEGAKVDFGFGLVSKGTDSPYQPFGAGRHRCIGEQFAYLQLGTIISTFVRHVEMRLPETGVPPPNYHAMITLPKAPRNILYRRRNFD

 

>CYP51F1 Cryptococcus neoformans var. neoformans B-3501A chromosome 1

56% to CYP51F1 Phanerochaete chrysosporium

AAEY01000001

CNBA0300"

ESTs gb|CF192489.1|CF192489,

gb|CF192488.1|CF192488, gb|CF191439.1|CF191439;

EAL23379.1"

MSAIIPQVQQLLGQVAQFFPPWFAALPTSLKVAIAVVGIPALII

GLNVFQQLCLPRRKDLPPVVFHYIPWFGSAAYYGEDPYKFLFECRDKYGDLFTFILMG

RRITVALGPKGNNLSLGGKISQVSAEEAYTHLTTPVFGKGVVYDCPNEMLMQQKKFIK

SGLTTESLQSYPPMITSECEDFFTKEVGISPQKPSATLDLLKAMSELIILTASRTLQG

KEVRESLNGQFAKYYEDLDGGFTPLNFMFPNLPLPSYKRRDEAQKAMSDFYLKIMENR

RKGESDHEHDMIENLQSCKYRNGVPLSDRDIAHIMIALLMAGQHTSSATSSWTLLHLA

DRPDVVEALYQEQKQKLGNPDGTFRDYRYEDLKELPIMDSIIRETLRMHAPIHSIYRK

VLSDIPVPPSLSAPSENGQYIIPKGHYIMAAPGVSQMDPRIWQDAKVWNPARWHDEKG

FAAAAMVQYTKAEQVDYGFGSVSKGTESPYQPFGAGRHRCVGEQFAYTQLSTIFTYVV

RNFTLKLAVPKFPETNYRTMIVQPNNPLVTFTLRNAEVKQEV

 

>CYP51F1 P450-40 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

46% to CYP51F1  Aspergillus fumigatus = CYP51F1

RO3G_11790.1

MAVISTLLPTLESIPLYAVLALGVFVIINILSQWFGPKNPKEPPVVFSWIPFMGNAIEFGINPIAFLQKCQKKYGDVFTFYMVGKRVTVFLNADGNQFVFNAKQNLTSAADAYNHMTKHVFGPEVVYDAPHSVFMEQKRFIKAGLNSESFRQHVPMIVEEVEGFFKNYKKPTGAFDAYHTLGELIICTASRCLMGKEIRASLDDSVAGLYYDLDQGFKPINFIFPNLPLPSYRKRDVARQKMTDLYSSIIARRKAENDFSNADLLQALMDANYKDGSNVPDHHIAGMMIAVLFGGQHTSATTSAWTLLELAARPDLIRDLREEQITKLGSLKADLTFDNLKELTLLDSCVRETLRLHPPIFQMMRRVTANKVVFEKTGHEIPKGNFLCAVPGVTQVDSQYFNEPLKYDPLRWINLTDPVHSMEAGDDSNIDYGFGAVGISSKNPFLPFGAGRHRCIGEQFGYLQIKTIIATIIRLFDIELEDGKGVPKSDYTSMVVVPERPSNIKYTWRE

 

>CYP51F1P Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

60% TO CYP51F1 Rhizopus oryzae

RO3G_08504.1 revised

NSIALYKNAKKKKSNVFTFHLVGKRVTLFLKAEDNQFVFNAKQNLISAANTHNYMTKHVFGPENVYSVPQCVFMEQK ()

XXXXXXXXXXNYSQHVL

MIVEEVESFFKDFKKPTSASDAYHILEKLIICTASRCLTDKETRVYLDDSAASLYYVLGQRFKSISFIFPNLPSHSYGKH

DVVRQKMADFYSNIIACRKVENDF (0)

SNADLLQSLMDAIYKNRIHISDHDITSMVIAILL &

E*YTFTTTSAWTLLELANRTSL &

IHGLRKEQITRLGSLKSSLTLDKFKKSTSLDDCIHKTLRLHPSIF*IRATADKIIYEK

TGHEISKDNLLCAVSSVTQINDQYYNSPLKYSRL**INLINPFHLREVGDDNDVDDNFDAVSVFSSNLFLPVGVVR &

HKCAGEQFGYQLLPL*PRLFDTKLEASRGVPKTNYTLMVVAPKRSSTIKYMERE

 

>CYP51F1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

73% to CYP51F1 Rhizopus oryzae

estExt_fgeneshPB_pg.C_10567

MAILSLITQVVPSITSVFLYGTLSLVVVFSIHILSQLVVPRDPNQPKLVFSWVPFLGNGVEFGMNPIGFIQKCQKLYGDV

FTFKLVGKSVTVCLGADGNQFVFNNKQNLSSAAEAYNDMTKHVFGFDVVYDAPHSVFMEQKRFIKAGLTLESFRAHVPLI

VKETTEFFKDFKKPTGYMDAYSTFSKLIICTASRCLMGKEIRSALHEGVADLYYDLDQGFQPINFMFPNLPLPSYRRRDV

AREKMAELYSKIIRRRKEENDTSNNDLLQALIESTYKDGTPIPDHHIAGMMIAVLFGGQHTSATTSAWTTLELANRPDLV

QALREEQIAKLGSLKADLTFENLKELTLMEACIKETLRLHPPIFQMMRKVIADKVVFEKTGHEIPKNDFLCAVPGATQVD

EQYFNEPLTYNPYRWIEKSDPVHQMEVGEDANADYGFGIVGISSRSPFLPFGAGRHRCIGEQFGYLQLKAVMATVIRLYD

LSLEEGKGVPESDYTSMVVVPKHPANVVYTCRE*

 

>CYP51F1 Batrachochytrium dendrobatidis JEL423 (chytrid)

43% to CYP51F1 Rhizopus oryzae

note this species has no obvious CYP61 sequence

BDEG_01891

MGLFFEAYESVRNVSGLPPDQFHTVVAITSLVFVLTVWLLSYALNQPLKSKDTGSEPPRVPVSIPFIGAAIAYGVDPIKF

LIECRQKYGDCFTFVMLGRKMTFCLGPDGNHYVFNVPVADATAEGAYKKLTVPVFGAGVVYDVHNAMFMEQKKFVKDAFN

SNSFNAYMLNIWNETNAFFNDWEDHYDANMIFDEMSELTIRTASHCLLGKEIRQQLHSTVAQLYHDLDSGLAPINVFFRW

LPLPVYFKRDRANKIMSKTFVDIIKKRRATNSTDNADLMQTLMFARYRDGTSMTDDEIAHLLIATLMGGQHTSSTTLCWM

LFELARRQDIVDKLLHEQSMVLTGKPDTPPEDLPEFTHEQIRQLTFLDCVMKETLRLHPPIHTVMRKVEKSMTYRGMTIP

AGHFICGSAAVSHLDPTRFPDPLKFEPSRFLNNDEGSGEWSINTIDIAQKSARSHFLPFGAGRHRCIGEAFAYIQNKTIL

SAFIRRYTHKLYVDPNTGKEHFPSSDYTSLITIPEKPARLSITRRSVKA

 

>CYP51F1 Fusarium oxysporum

92% to CYP51F1   Fusarium graminearum

FOXG_00394

MGLLQELASHPLAQQYQELPLGQQIGIGFGAFIILSVVLNVLNQLLFKNRNEPPLVFHWFPFVGSTITYGMDPPKFFKEN

RAKHGDVFTFVLLGKKTTVAVGPTGNDFILNGKLKDVSAEEIYTVLTTPVFGKDVVYDCPNAKLMEQKKFMKIALTTEAF

RSYVPIISAEVRDYFKKSPDFKGKSGIVDIPKKMAEITIFTASHALQGSVIRNKFDESLAALYHDLDMGFTPINFMLHWA

PLPWNRKRDHAQRTVAKIYMDTIKERRAKDNDDTEHDMMKHLMNSTYKNGTPVPDHEVAHMMIALLMAGQHSSSSTSSWI

MLRLAQYPHIMEELYQEQVRELGADLPPLTYDNLAKLPLNQAIIKETLRLHAPIHSIMRAVKSPMPVPGTKYTIPTSHTL

LAAPGVSATDSAYFPNPDEWDPHRWEVDSPNFPRMATRGDDEEKIDYGYGLVSKGSASPYLPFGAGRHRCIGEHFANAQL

QTIVAEVVREFKFRNVDGGNTLIDTDYASLFSRPLEPANIHWERRQQ*

 

>CYP51F1 Fusarium verticillioides

98% to CYP51F1 Fusarium oxysporum

FVEG_01123

MGLLQELASHPLAQQYQELPLAQQIGIGFGAFVVLSVVLNVLNQLLFKNRNEPPLVFH

WFPFVGSTITYGMDPPKFFRENRAKHGDVFTFVLLGKKTTVA

VGPTGNDFILNGKLKDVSAEEIYTVLTTPVFGKDVVYDCPNAKLME

QKKFMKIALTTEAFRSYVPIISSEVRDYFKKSPDFKGKSGIVDIPKKMAEITIF

TASHALQGSVIRNKFDESLAALYHDLDMGFTPINFMLHWAPLPWNRKR

DHAQRTVAKIYMDTIKERRAKDNDDTEHDMMKHLMNSTYKNGTPVPDHEVAH

MMIALLMAGQHSSSSTSSWIMLRLAQYPHIMEELYQEQVRELGADLPPL

TYDDLAKLPLNQAIIKETLRLHAPIHSIMRAVKSPMPVPGTKYTIPTSHTL

LAAPGVSATDSAYFPNPDEWDPHRWEADSPNFPRMANRGDDEEKIDYGYG

LVSKGSASPYLPFGAGRHRCIGEHFANAQLQTIVAEVVREFKFRNVDGGN

TLIDTDYASLFSRPLEPANIHWERRQQ*

 

>CYP51F2 Fusarium graminearum FG04092.1 AACM01000179 FGcontig1.179_scaffold2

MFHLLIYPLWVLVALFAVIIANLLYQQLPRRPDEPPLVFHWFPFFGNAVA

YGLDPCGFFEKCREKHGDVFTFILFGRKIVACLGVDGNDFVLNSRLQDAN

AEEVYGPLTIPVFGSDVVYDCPNSKLMEQKKFVKFGLTQKALESHVQLIE

REVLDYVETDPSFSGRTSTIDVPKAMAEITIFTASRSLQGEEVRRKLTAE

FAALYHDLDLGFRPVNFLFPWLPLPHNRKRDAAHIKMREVYMDIINDRRK

GGIRTEDGTDMIANLMGCTYKNGQPVPDKEIAHMMITLLMAGQHSSSSAS

SWIVLHLASSPDITEELYQEQLVNLSVNGALPPLQYSDLDKLPLLQNVVK

ETLRVHSSIHSILRKVKRPMQVPNSPYTITTDKVIMASPTVTAMSEEYFE

NAKTWNPHRWDNRAKEEVDTEDVIDYGYGAVSKGTKSPYLPFGAGRHRCI

GEKFAYVNLGVIVATLVRNFRLSTIDGRPGVPETDYTSLFSRPAQPAFIR

WERRKKI*

 

>CYP51F2 Aspergillus nidulans AN1901.1 64% to 51F2, 60% to 51F1

MLSPTLFSAYVLVGAIVAVLVNVIRQIFFRNKNEPPMVFHWVPFVGSTIS

YGMNPYKFFFSCREKYGDIYTFVMLGKKMTVYMGVKGNDFILNGKLKDLN

AEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLSQSALESHVPLIE

KEVLDYIKTSPRFKGDSGVLDAPAAMAELTIYTAGSALQGKEVRKKLTAE

FADLFHDLEMGFTPINFILPWAPLPQNRKRDIAHARMRETYMEIINQRRK

NPDAQDHDMIWNLMHSTYKNGNPVPDKEIAHIMITLLMAGQHSSSSISAW

ILLRLASEPQILEELYQEQLANLKRDPRTGAFEPLQYKDLDLLPLHQNVI

KETLRVHLSIHSILRKVKNPIPVPDTPYIIPTSHTLLASPGATALSDEYF

PNANMWDPHRWENQRPDKEDEEGELIDYGYGAVSKRMSSPYLPFGGGRHR

CIGEKFAYVNLGVIVATIVRNLKLYNVDGKTGVPATDYSSMFMGPMKPAV

VGWERRFPARS*

 

>CYP51F2  Aspergillus niger CBS 513.88

         XM_001394187.1, CAK40582.1

         80% to CYP51F2 Aspergillus fumigatus

MALLAVAGVYAFAALLVAIVLNVTRQLLFRNEKEPPVVFHWIPF

LGSTISYGMDPYTFFFSCRKKYGDIFTFVLLGQKTTVYLGVQGNDFILNGKLKDVSAE

EVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKFGLTQAALESHVQLIEKETLDYLRDS

PRFNGASGVIDIPAAMAEITIYTAARALQGEEVRKKLTAEFAELYHDLDKGFSPINFM

LPWAPLPHNQKRDAAHARMREIYTDIINERRKNPDEEKSDMIWNLMHCTYKSGQPVPD

KEIAHMMITLLMAGQHSSSSISSWIMLRLASEPQVLEELYQEQLASLSNRNGVFEPLQ

YQDLDKLPFLQSVIKETLRIHSSIHSIMRKVKNPLPVPGTSYIIPEDHVLLASPGVTA

LSDEYFPNATRWDPHRWENQPDKEEDGEMVDYGYGSVSKGTASPYLPFGAGRHRCIGE

KFAYVNLGVIIATIVRHLKLFNVDGRKGVPGTDYSTLFSGPMKPAIVGWERRFPDHSK

GSLN

 

>CYP51F2  Aspergillus terreus NIH2624

         XM_001215095.1

         78% to CYP51F2 Aspergillus fumigatus

         ATEG_05917.1

MSLLTITSYAAVGILALIAWNVIRQLLFQNKSEPPVVFHWIPFL

GSTISYGIDPYAFFASCRQKYGDIFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEE

VYSPLTTPVFGSDVVYDCPNAKLMEQKKFIKYGLTQAALESHVQLIEREVLDYLRTSP

NFHGASGVVDISAAMAELTIFTAGRALQGEEVRRKLTAEFADLYHDLDKGFTPINFML

PWAPLPHNRKRDAAHARMRAIYMDIIRERRAHKNPDQVEAGSDSDMIWNLMRCTYKDG

QPVPDKEIAHMMITLLMAGQHSSSSISAWIFLRLASEPRVLEELYQEQVASLGKPDAD

GVFPPLQFRDLDRLPLHQNVVKETLRLHSSIHSIMRKVKNPLPVPGTPYVVPTSHVLL

ASPGVTATSDEYFPNASRWDPHRWENQAEPDDDGEMVDYGYGRVSKGTASPYLPFGGG

RHRCIGEKFAYINLGVIVATVVRHLKLANVDGRKGVPQTDYSSLFSGPVKPAIIGWER

RFPTA

 

>CYP51F2  Aspergillus clavatus NRRL 1

         XM_001271578.1

         ACLA_046180

         81% to CYP51F2 Aspergillus fumigatus

MLSLTLFGLYLVSATAVVILVNVVYQHLFRLRNRTEPPMVFHWI

PFIGSTITYGIDPCKFFFACREKYGNIFTFILLGQKVTVYLGVEGNEFILNGKLKDVN

AEEVYSPLTTPVFGSDVVYDCPNSKFMEQKKFIKFGLTQSALEAHVPLIEKEVLDYLE

TSPRFQGTSGLVDIAAAMAEITIFTAARALQGEEVRSKLTAEFADLYHDLDRCFTPVN

FMFPWAPLPRNKKRDAAHVRMRAIYVDIINQRRRDGGEDTQKSDMIWNLMNCSYKNGQ

QVPDKEIAHMMITLLMAGQHSSSSIGSWIMLRLASQPEVLEKLYQEQLDKLAQGGPGS

NLRPLQYKDLELLPYHQHVIRETLRLHSSIHSILRKVKNTLSVPGTSYVIPPGRVLLA

SPGVTALSDEHFPNASRWDPQRWENQTTKEDSGEMVDYGYGAVSKGTASPYLPFGAGR

HRCIGEKFAYVNIGVILATLVRHLRLSNMDGKEGVPATDYSSLFSGPMKPSIIQWGKR

SNDLSK

 

>CYP51F2  Ajellomyces capsulatus NAm1

         XM_001540591.1

         70% to CYP51F2 Aspergillus fumigatus

MSWFTIGTFGVLAFVAAVTLNLVYQLLFRFLNKTRPPLVFHWIP

FLGSTIRYRTDPYKFFFSCRQKEASRLFQHGDIFTFVLLGRPTTVYLGIKGNEFILNG

KLKDVNAEEVYGPLTTPVFGPDVVYDCPNSKLIEQKKFIKYGLTQAALESHVPLIEKE

VMDYLDSSPNFLGASGEVDISAVMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLDK

GFSPINFMLPWAPLPHNKKRDAAHTLMHAIYLDIIEKRRRAARNANGSQTQDMIENLM

QCTYKNGQKLPDKEIANIMISLLMAGQHSSSTTSSWIMLHLASEPAVVEQLYQEQLDN

LPRTGPNGSLGPLQYGDLNRLPLHRNVIRETLRLHTSIHSLLRKVMNPMPVAGTPYVI

PPSHVVLSAPGVTALSDEYFPNPTMWDPNRWETQEPKVDEKEDMVDYGYGTISKGTSS

PYLPFGAGRHRCIGEKFAYVNLTVIVAIMVRHLQFSNIGGKTGVPRTDYSSMFSAPMK

PARINWKRRTAKSG

 

>CYP51F2 Histoplasma capsulatum G217B

94% to CYP51F2  Ajellomyces capsulatus NAm1

two identical sequences

ABBT01000205.1 Ajellomyces capsulatus G217B 481620-479996 (-)

ABBT01000207.1 Ajellomyces capsulatus G217B 78633-77009 (-)

HCB05685.1 identical to HCB06140.1

MSWSTIGTFGVLAFVAAVAFNLVYQLLFHFLNKTRPPLVFHWIPFLGSTIRYGTDPYKFFFSCRQKHGDIFTFVLLGRPT

TVYLGIKGNEFILNGKLKDVNAEEVYSTLTTPMFGPDVVYDCPNSKFIEQKKFIKYGLTQEALESHVPLIEKEVMDYLDS

SPNFLGASGEVDISAVMAEITIFTAGSALQGEEVRSKLTAEFAVLYHDLDKGFSPINFMLPWAPLPHNKKRDAARALMHA

IYLDIIEKRRRAARNANGSQTQDMIENLMQCTYKNGQKLPDKEIANIMITLLMAGQHSSSTTSSWIMLHLASEPAVAEQL

YQEQLDNLPRTGPNGSLAPLQYGDLDRLPLHRNVIKETLRLHTSIHSLLRKVMNPMPVAGTPYVIPPSHIVLSAPGVTAL

SDEYFPNPTMWDPNRWETQEPKVDEKEDMVDYGYGAISKGTSSPYLPFGAGRHRCIGEKFAYVNLTVIVAIMVRHLQFSS

IGGKTGVPRTDYSSMFSGPMKPARINWKRRTAKSG

 

>CYP51F2   Penicillium digitatum strain GL-01

          DQ355161.1, also AJ439081 (5 aa diffs)

          67% to CYP51F2 Aspergillus fumigatus

MDLVPLVTGQIKCIAYYTTGLVLASIVLNVIKQLVFYNRKEPPV

VFHWIPFIGSTVAYGMDPYQFFFASRAKYGNIFTFILLGKKTTVYLGVEGNEFILNGK

LKDVNAEEIYGKLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLSQEALESYVPLIADEI

SSYIKSSPSFKGQSGTIDLVPAMAEITTFTAARTLQGEEVRSKLTTEFAKLFHDLDLG

FTPINFMLPWAPLPQNRKRDRAHRRMREIYVDIIQARREAGEEANDNGRDKTKGTDMI

SNLMRCVYRDGTPIPDKEIAHLMITLLMAGQHSSASISCWILLRLASQPEMTEKLFAE

QVNNLGADLPLLQYKDLDKLPLHRNVIKETLRLHSSIHTLMRKVKNPMPVPGTDFVIP

PSHTLLSSPGVTARDERHFRDPLRWDPHRWGSRVEAEDSSDTVDYRYGAVSKGTRSPY

LPFGAGRHRCIGEKFAYLNLGVIIATLLREFRFFNPEGMEGVPDTDYSSLFSRPMQPA

TVRWEVRS

 

>CYP51F2 Nectria haematococca fgenesh1_pg.scaffold_7000156

57% to CYP51F1 like seq fgenesh1_pg.scaffold_2000426,

Necha1/scaffold_7:419968-421550

80% to Fusarium graminearum  CYP51F2

FG04092.1 AACM01000179 FGcontig1.179_scaffold2

Note only one intron, not two as seen in CYP51F1

This gene model seems correct DRN 2/4/06

MLLLVWYPVLAIGAFLGIILIHALRQKLFRKAGEPPLIFHWLPFIGNAVSYGLDPCNFFMKCREK (0)

HGDVFTFVLFGRKIVCCLGVEGNDFVLNSRLQDANAEEIYGPLTIPVFGSDVVYDCPNSKFMEQKKFVKFGLTQK

ALESHVQLIEREVLDYIHAVPAFSGSEGTVDISNAMAEITIFTAARSLQGEEVRRKLTAEFAALYHDLDL

GFKPVNFLFPWAPLPHNRKRDAAHAKMRDIYMDIINKRRAEGGDLGEYSDMIANLMGCTYKNGQPVPDKE

IAHMMITLLMAGQHSSSSASSWIMFRLASRPDIAEELYQEQLTHLSVDGYLPPLQHSDLDKLTLLQNVVK

ETLRVHSSIHSILRKVKTPMQAPGTQYTITTDKVILASPTVTALSEEYFSNARTWDPHRWDNKEKEEVAG

DDVVDYGYGKVSKGTKSPYLPFGAGRHRCIGEKFAYVNLGVIVATMVRNFKLSTMDGKSGVPATDYNSLF

SRPVQPAYIRWERRKA*

 

>CYP51F2v1 Aspergillus fumigatus

          GenEMBL AAF32372.1 also AAK73659.1

          cytochrome P450 sterol 14 alpha-demethylase variant

MVPMLWLTAYMAVAVLTAILLNVVYQLFFRLWNRTEPPMVFHWVPFLGSTISYGIDPYKFFFACREKYGD

IFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLT

QSALESHVPLIEKEVLDYLRDSPNFQGSSGRMDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDL

DKGFTPINFMLPWAPLPHNKKRDAAHARMRSIYVDIINQRRLDGDKDSQKSDMIWNLMNCTYKNGQQVPD

KEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPAGPDGSLPPLQYKDLDKLPFH

QHVIRETLRIHSSIHSIMRKVKSPLPVPGTPYMIPPGRVLLASPGVTALSDEHFPNAGCWDPHRWENQAT

KEQENDEVVDYGYGAVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVILATIVRHLRLFNVDGKKGVPETD

YSSLFSGPMKPSIIGWEKRSKNTSK

 

>CYP51F2v2 Aspergillus fumigatus Af293

          GenEMBL XP_752137.1 also EAL90099.1

          14-alpha sterol demethylase variant

          74% to 51F2 A. nidulans 62% to 51F1 A. nidulans

          only 5 aa diffs to CYP51F2v1, may be another gene or an allele

MVPMLWLTAYMAVAVLTAILLNVVYQLFFRLWNRTEPPMVFHWVPYLGSTISYGIDPYKFFFACREKYGD

IFTFILLGQKTTVYLGVQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLT

QSALESHVPLIEKEVLDYLRDSPNFQGSSGRVDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDL

DKGFTPINFMLPWAPLPHNKKRDAAHARMRSIYVDIITQRRLDGEKDSQKSDMIWNLMNCTYKNGQQVPD

KEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPAGPDGSLPPLQYKDLDKLPFH

QHVIRETLRIHSSIHSIMRKVKSPLPVPGTPYMIPPGRVLLASPGVTALSDEHFPNAGCWDPHRWENQAT

KEQENDKVVDYGYGAVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVILATIVRHLRLFNVDGKKGVPETD

YSSLFSGPMKPSIIGWEKRSKNTSK

 

>CYP51F2  Aspergillus oryzae

         GenEMBL BAE57417.1

         77% to CYP51F4, 75% to CYP51F2 Aspergillus nidulans

MASFTLVSAYAAAGLLAIIVLNLLRQLLFRNKTDPPLVFHWIPFLGSTVTYGMDPYAFFFSCRQKYGDIF

TFILLGRKITVYLGIQGNEFILNGKLKDVNAEEIYSPLTTPVFGSDIVYDCPNSKLMEQKKFIKFGLTQA

ALESHVPLIEKEVLDYLKTSPNFKGTSGRVEITDAMAEITIFTAGRALQGEEVRKKLTTEFADLYHDLDR

GFTPINFMLPWAPLPRNRKRDAAHARMREIYMDIINERRKNPDRETSDMIWNLMHCTYKNGQPLPDKEIA

HMMITLLMAGQHSSSSISSWIMLRLASEPAVMEELYQEQITKLSPDGRTLPPLQYRDLDLLPLHQNLIKE

TLRLHLSIHSLMRKVKNPMPVPGTPYVVPADHVLLASPGVTALSDEYFPNASRWDPHRWENRVEKEDEED

IVDYGYGTVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVIVATMARHMKLFNVDGKKGVPATDYSSMFSG

PSKPAIIGWERRFPEKS

 

>CYP51F2  Aspergillus flavus

99% to CYP51F2  Aspergillus oryzae, 1 aa diff

AFL2G_02771

MASFTLVSAYAAAGLLAIIVLNLLRQLLFRNKTDPPLVFHWIPFLGSTVTYGMDPYAFFFSCRQKYGDIFTFILLGRKIT

VYLGIQGNEFILNGKLKDVNAEEIYSPLTTPVFGSDIVYDCPNSKLMEQKKFIKFGLTQAALESHVPLIEKEVLDYLKTS

PNFKGTSGRVEITDAMAEITIFTAGRALQGEEVRKKLTAEFADLYHDLDRGFTPINFMLPWAPLPRNRKRDAAHARMREI

YMDIINERRKNPDRETSDMIWNLMHCTYKNGQPLPDKEIAHMMITLLMAGQHSSSSISSWIMLRLASEPAVMEELYQEQI

TKLSPDGRTLPPLQYRDLDLLPLHQNLIKETLRLHLSIHSLMRKVKNPMPVPGTPYVVPADHVLLASPGVTALSDEYFPN

ASRWDPHRWENRVEKEDEEDIVDYGYGTVSKGTSSPYLPFGAGRHRCIGEKFAYVNLGVIVATMARHMKLFNVDGKKGVP

ATDYSSMFSGPSKPAIIGWERRFPEKS*

 

>CYP51F2  Neosartorya fischeri NRRL 181

         XM_001267337.1, NFIA_109350

         97% to CYP51F2 Aspergillus fumigatus

MVPMLLLTAYMAVAMLTAILLNVVYQLFFRLWNRTEPPMVFHWV

PFLGSTISYGIDPYKFFFACREKYGDIFTFILLGQKTTVYLGVQGNEFILNGKLKDVN

AEEVYSPLTTPVFGSDVVYDCPNSKLMEQKKFIKYGLTQSALESHVPLIEKEVLDYLR

NSPNFQGSSGQVDISAAMAEITIFTAARALQGQEVRSKLTAEFADLYHDLDKGFTPIN

FMLPWAPLPHNKKRDAAHARMRSIYIDIINQRRLDGEKDSQKSDMIWNLMNSTYKNGQ

QVPDKEIAHMMITLLMAGQHSSSSISAWIMLRLASQPKVLEELYQEQLANLGPVGPDG

SLPPLQYKDLDKLPFHQHVVRETLRLHSSIHSLMRKVKSPLPVPGTPYMIPPGRVLLA

SPGVTALSDEHFPNAGCWDPHRWENQAAKEQENDEVVDYGYGAVSKGTSSPYLPFGAG

RHRCIGEKFAYVNIGVILATIVRHLRLFNVDGKKGVPETDYSSLFSGPMKPSIIGWEK

RSKDTSK

 

>CYP51F2 Magnaporthe grisea MG04628.4  62% to CYP51 Penicillium AACU01001139 cont2.870

MAFFFPSAPVWVYSAGAALLFIIGSIILNFIWQQLPRPKSEPPLVFHWLP

FIGNAVSYGMDPYRFYSQCREKHGDVFTFVLFGRRMTVFLGVQGNDFILN

GKLQDLNAEEIYSPLTTPVFGSDIIYDCPNSKLMEQKKFVKFGLTQKALD

SYVPLIEREVLDYIESSPVFQAGNHGIVDIPSMMAEITIFTASRTLQGPE

VRKKLTGEFARLYHDLDLGFRPINFLAPWAPLPQNRRRDVAHARMRDVYM

DLINKRRRQKDDQEEEEEAEPDMIRHLMGSCVYKNGQALPDKEIAHMMIT

LLMAGQHSSSSSSAWIMLRLASRPDIAEEVYQEVQRLGHASLQHSDLDKL

PLLANVVKETLRVHSSIHSIMRKVKRPMRIPGSDYVVTPGKVLVSAPIMT

HLDEEHFRDARAWEPHRWDDAVDAQDDEIVDYGYGATSKGTKSPYLPFGA

GRHRCIGEKFAYLNLAAIVSTLVRNFKFSTLDGKATVPPTDYTSMFSRPM

QPATVRWERRSPKTA*

 

>CYP51F2 Fusarium oxysporum

83% to CYP51F2   Fusarium graminearum

FOXG_11545

MFSLLYYPLWAFASCLVIITLNVLYQKLPRNANEPPLVFHWLPFVGNAVAYGLDPYGFFVKCREK HGDVFTFILFGRKIV

ACLGVDGNDFVLNSRIQDANAEEIYSPLTTPVFGSDVVYDCPNSKLMEQKKFVKFGLTQKALESHVQLIEREVLEYIQAV

PSFSGKSGTVDVSKAMAEITIFTAARSLQGEEVRRKLTAEFAALYHDLDLGFTPVNFLFPWLPLPHNRRRDAAHAKMREI

YMDIINGRRRGVGDLEKGTDMIANLMNCEYKNGQPIPDKEIAHMMITLLMAGQHSSSSASSWIILHLASSTDIAEELYQE

QLINLSADGVLPPLQYTDLDKLPLLQNVVKETLRVHSSIHSILRKVKRPMQAPGSPYTITTDKVLLASPTVTALSEEHFT

DAQRWNPHRWDNKPQEEAVTDDVIDYGYGAVSKGTKSPYLPFGAGRHRCIGEKFAYVNLGVIVATLVRNFRLSTLDGKPG

IPATDYTSLFSRPAQPAYINWERRRA*

 

>CYP51F2 Fusarium verticillioides

94% to CYP51F2 Fusarium oxysporum

FVEG_10277

MFSLLYYPLWAFASCLAIITLNVLYQKLPRNANEPPLVFHWLPFFGNAVAYGLDPYGFFVKCREK (0)

HGDVFTFVLFGRKIVACLGVDGNDFVLNSRIQDANAEEIYGPLTTP

VFGSDVVYDCPNSKLMEQKKFVKFGLTQKALESHVQLIEREVLEYIQAVP

SFSEKSGTVDVSKAMAEITIFTAARSLQGEEVRRKLTAEFAALYHDLDLGFT

PVNFLFPWLPLPHNRRRDAAHSKMREIYMGIINERRRGGGDLEKRTDM

IANLMSCAYKNRQPIPDKEIAHMMITLLMAGQHSSSSASSWIVLHLASSPDI

TEELYQEQVINLSASGALPPLQYSDLDKLPLLQNVVKETLRVHSSIHS

ILRKVKRPMQAPGPPYTITTDKVILASPTVTALSEEHFPDAQRWNPHRWDNKP

QEEAVTDEVIDYGYGAVSKGTKSPYLPFGAGRHRCIGEKYAYVNLGV

IVATLVRNFRLSTLDGKPGVPATDFTSLFSRPAQPAYIKWERRKA

 

>CYP51F3 Fusarium graminearum FG11024.1 AACM01000457 FGcontig1.457_scaffold8

MESLYETLRTLPLSVSIPLTTSIIIILSIVTNVVKQLWFPNPHRPPVVFH

IFPFIGSTVQYGIDPYAFFFDCRDKYGDCFTFILLGKSTTVFLGPKGNDF

ILNGKHADLNAEDVYGKLTTPVFGEEVVYDCSNARFMDQKRLLKLGLTTD

SLRCYIPKFVKEVEDYVKNSPYFKGDTGIVNITEVMAEITIYTASGSLLG

NEVRSMFDSTFATLYRHLDDGFQPINFVMPGLPLPQNFRRNHARKVMEKL

FSDIISKRRETGNQGDETDMIWMLMNAQYKDGEPLPDHHAARMLIAILMG

GQHNTAVSGAWLLLNLAHKPHLVQELYEEQTQVLGSPQEPLTWENLQKLT

LNGQVIKETLRLHSPIHSILRQVKSPMRVPGTEWVVPPSHTLLSSPGTMA

RSEEFFPRPSEWDPHRWDKIEPLVKTAEDGQTVDYGFGVMSKSVSSPYLP

FGAGRHRCVGENYAYAQLGAIVATFIRLVHIEQPDPKAPLPAPDYSSMFS

RPMNPAEIRWRRRETVE*

 

>CYP51F3 Nectria haematococca fgenesh1_pg.scaffold_6000765

52% to fgenesh1_pg.scaffold_2000426,

Necha1/scaffold_6:2267196-2268844

81% to Fusarium graminearum  CYP51F3

FG11024.1 AACM01000457 FGcontig1.457_scaffold8

Note two exons, but the second exon is not seen in CYP51F1 or CYP51F2

This gene model seems correct DRN 2/4/06

MESLYGSFRALPLSISIPFTVGLVVVIAIARNVIGQLYFPDRNRPPVVFHVFPFIGSSVQYGIDPYKFFF

DCKAKYGDCFTFILLGKPTTVFLGQKGNEFILNGKHADLNAEEVYGKLTTPVFGKEVVYDCSNARFMDQKR (0)

LLKLGLTTEALRAYIPKFVKEVEDYVNTSPYFKGETGQVNITEVMAEITIYTASGSLLGNEVRSMFDST

FALLYRHLDDGFQPINFVMPWLPLPQNRRRDHARNVMEGLFANIIRQRREQGNQGDETDMIWTLMDAQYK

DGTVLPDIHVARLLIAILMGGQHNTAASGAWLFLNLAHKPHLVEEMYQEQLEVLGSPPAPLTWENVQKLT

LNGQVIKETLRLHSPIHSIMREVKSPMPVPGTEWVVPPSHTLLASPGTLARSEDFFPRPLEWDPHRWDKA

DPVEGPVDDSQKVDYGFGMMSKAVSSPYLPFGAGRHRCVGEHYAYAQLGAIVATFVRLCHMEQVDPKAPI

PATDYS (1)

SMFSRPMNPAVIKWRRRKPE*

 

>CYP51F3 Fusarium oxysporum

87% to CYP51F3 Fusarium graminearum

FOXG_13138, note: gc boundary at DQKR

MEALYSNWRSFPLSLSVPITAFLIIAIATITNVIKQLWFPNPHRPPVVFHIFPLIGSTVQ

YGIDPYKFFFDCQAKYGDCFTFILLGKSTTVFLGPKGNDFILNGKHADLNAEDVYGKLTT

PVFGKEVVYDCSNARF MDQKR (0)

LLKLGLTTDSLRCYIPKFVKEVEEYIATSPYFKGSTGIVNITEVMAEITIYTAAGSLLGNEVRSMFDSTFATLYR

HLDDGFQPINFVMPGLPLPQNFRRDHARKVMEELFSDIIRKRREIGNQGGETDMVWTLMNAKYKDGEDLPDHHAARMLIA

ILMGGQHNTAASGAWLLLNLAHKPHLVQELYDEQLEVLGSPQEPLTWENLQKLTLNGQVIKETLRLHSPIHSILRQVKSP

MRVPGTDWVVPPSHTLLASPGTQARSEEFFPRPMEWDPHRWDKIESLEDSKGNGETVDYGFGVMNKSVSSPYLPFGAGRH

RCVGENYAYAQLGAIIATFVRLLHIEQPDPNAPLPAPDYSSMFSRPMNPAVIRWTRRNTEAN*

 

>CYP51F3 Fusarium verticillioides

95% to CYP51F3 Fusarium oxysporum

FVEG_12391

MDALYTNWRSLPLSLSIPITAFLIIVIATITNVIKQLWFPNPHRPPVVFHIFPLIGSTVQ

YGIDPYKFFFDCQAKYGDCFTFILLGKSTTVFLGPKGNDFILNGKHADLNAEDVYGKLTT

PVFGREVVYDCSNARF

MDQKRLLKLGLTTESLRCYIPKFVKEVEDYIATSPYFKGNTGIVNITEVMA

EITIYTAAGSLLGNEVRSMFDSTFATLYRHLDDGFQPINFVMPGLPLPQ

NFRRDHARKVMEELFSDIIRKRREMGNQGDETDMVWTLMNAKYKDGEDLPN

HHAARMLIAILMGGQHNTAASGAWLLLNLAHKPHLVKELYDEQVEVLGS

PQEPLTWENLQKLTLNGQVIKETLRLHSPIHSILRQVKSPMRVPGTDWVV

PPSHTLLASPGTQARSEEFFPRPMEWDPHRWDKIESLDDAKNGETVDYGF

GMMSKSVSSPYLPFGAGRHRCVGENYAYAQLGAIIASFVRLLHIEQPDPK

APLPAPDYSSMFSRPMNPAVIRWTRRNAETG

 

>CYP51F4  Aspergillus oryzae

         GenEMBL BAE63554.1

         72% to 51F2 Aspergillus nidulans, 77% to 51F2 Aspergillus oryzae

         60% to 51F1 Aspergillus oryzae

MSWPRIGAYALLAFVAIMALNVTYQFLFRMLNKTRPPLVFHWIPFIGSTIHYGTDPYGFFFSCREKYGDI

FTFILLGRPTTVYLGTQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLIEQKKFIKFGLSQ

AALEAHVPLIEKEVEDYLAMSPNFHGTSGEVDIPAAMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLD

KGFTPINFMLPWAPLPHNKKRDAAHARMRAIYIDIINKRRNAGDNVPEKLDMIGNLMQCTYKNGQPLPDK

EIAHIMITLLMAGQHSSSSISSWIMLRLASQPAVVEELYQEQLANLERTGPNGSLAPLQYKDFDNLPLHQ

NVIRETLRLHSSIHSLLRKVKNPLPVPGTPYVIPTSHVLLAAPGVTALSDEYFPNAMAWDPHRWETQAPQ

ENNKDDIVDYGYGAMSKGTSSPYLPFGAGRHRCIGEKFAYLNLAVIVATMVRHLRFSNLDGQTGVPDTDY

SSLFSGPMKPARIRWERRAAKSG

 

>CYP51F4 Aspergillus flavus

99% to CYP51F4  Aspergillus oryzae

AFL2G_10953

MSWPRIGAYALLAFVAIMALNVTYQFLFRMLNKTRPPLVFHWIPFIGSTIHYGMDPYGFFFSCREKYGDIFTFILLGRPT

TVYLGTQGNEFILNGKLKDVNAEEVYSPLTTPVFGSDVVYDCPNSKLIEQKKFIKFGLSQAALEAHVPLIEKEVEDYLAM

SPNFHGTSGEVDIPAAMAEITIFTAGSALQGEEVRSKLTTEFAVLYHDLDKGFTPINFMLPWAPLPHNKKRDAAHARMRS

IYIDIINKRRNAGDNVPEKLDMIGNLMQCTYKNGQPLPDKEIAHIMITLLMAGQHSSSSISSWIMLRLASQPAVVEELYQ

EQLANLERTGPNGSLAPLQYKDFDNLPLHQNVIRETLRLHSSIHSLLRKVKNPLPVPGTPYVIPTSHVLLAAPGVTALSD

EYFPNAMAWDPHRWETQAPQENNKDDIVDYGYGAMSKGTSSPYLPFGAGRHRCIGEKFAYLNLAVIVATMVRHLRFSNLD

GQTGVPDTDYSSLFSGPMKPARIRWERRAAKSG*

 

>CYP51F5 P450-46 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

62% to P450-40

42% to CYP51F1 Ustilago maydis in the CYP51 family

45% to CYP51F1   S. pombe

RO3G_16595.1

MAIISTLLSETSLSSLLYKAIVFLATYYVIHLLSQFQPKDPKAVPLVPSWIPFFGNAIEFGKNPIEFLQTCQKKYGDVFTFRLLNKRVTACLGPDGNQFVFNAKQEVASAAAAYNHMTKYVFGNDIVFDTAHSVFMEQKRMIKSGLNIEAFRKDVPLIIEECDAFFDGLEPQGEMDLYKMFGTLIIYTASRTLLGPEIRQALDSGVSELYYDLDQGFRPINFMFPNLPLPSYRRRDEAREKLAKIYAGIIQKRRQSEHGESDLLQTLIEARYKDGSAVPDAQICGILTAALFGGQHTSSTTAAWTILELAQRPDIIQALRQEMITQCGSLEVDFTYDHLERLTLLEHVVKETLRLHPPIFNMMRRVVAPKCVFSGREIPQGDYLLAAPGVTQLDPHYFHQPTVWDPYRWSQLKDPVHQLEQGEDANADYGFGVVGISSKSPFLPFGAGRHRCIGEKFGYLQLKTIIGTFVKRFDVEPLSHTVPKPDYTSMVVVPENSHIRYRARKQ

 

>CYP51F6 Kluyveromyces polysporus

Kpol_1023p61

65% to CYP51F1 S. cerevisiae

MELVKSLLLFIISFSFWQIVTIIIVTPLAYNIFWQVYESFRTDRPPLVMHWVPWVGNYASYNLKPYKFLQDCQKKHGDIF

SFVMLGQIITVYLGPNGNDFIFNANVDEVSAKDVYSRMTTPVFGKGVIYDCDHDKLIEQKRFVKKALTIESLKKYVPLIQ

DEIQKYFTNSKFFRLGKYNSGITNVMATQPEMTIFTSSRTLLGEQLRKKLDTDFAYLYSDLDKGFTPINFVFKNLPLEHF

RSRDNAHKLISTTYLSLIKERRATNDIKDRDLIDSLLKNSTYKDGTKMTDQEIANLLIGVLMGGQHTSAATSAWFLLHLA

EKPQIQEELYKEVMEVLDNGRLPLTYESLQKMKLVNQTIRETLRLHHPLHSLFRKVRKNIPIPRTNYIIPKHRLLLVSPG

YTHLQDKYFPNAKEFDIHRWSDNQINESVEHIDYGFGKISKGTSSPYLPFGGGRHRCIGEDFAYCQLGTLLVTFVTKIKW

SLPENKAIPETDYQSMVTLPLGSAHILWEKR

 

>CYP51F7 Uncinocarpus reesii

58% to CYP51F   Uncinocarpus reesii from PPVV on

62% to Aspergillus terreus CYP51F1

UREG_00593.1, model is short. Seq. runs into a gap, missing last exon

MESFLVFSPTTQLLPAVLIVGVLTVFIGNLLLQFSSRGGAK

PPVVFHWVPFVGSAISYGLDPYRFFASCREK ()

YGDIFTFVLLGRKITVYLGVKGNDFILNGKLQDVNAEEVYSPLTTPVFGSDVIYDCPNQKLMEQKK

FIKFGLSSEALASYVPLIQHEVEQYIATSPHFRGESGTVNIAQ

IMAEITILTAARTLQGEEVRSKLDSSFANYYHDLDAGFTPINFLLPWAPLPHNRKRDAAH

KKMRDVYMGIIKQRRASGQTRGHDMLWNLMDNCVYKDGSALPDKEIAHIMITLLMAGQHT

SSAVSSWAMLQLAAHPEIGEDLYQEQIRVLESSQALTLRDIDRLTLNQMVLKETLRLHSP

IHSIMRKAKNTMSIPGTDLVVPESHILLSAPGFTARSEDHFRDAVEWNPRRWLSAPEPSE

DDDEKIDYGYGVTMKGTKGPYLSFGGGRHRCIGEKFAYLNLG (1)

 

>CYP51F7 CIMG_00573.2 Coccidioides immitis

84% to CYP51F7  Uncinocarpus reesii (ortholog to second CYP51 in this species)

CIMG_00573.2

MESLPFPSPTVQLLPTVVIIGVLSIFIGNLLRQFAPHRGAKPPVVFHWVPFIGSAISYGLDPYRFFTSCREKYGDIFTFV

LLGRKVTVYLGVKGNEFILNGKLQDVNAEEVYSPLTTPVFGSDVIYDCPNQKLMEQKKFIKFGLSSEALASYVPLIQHEV

EQYIASSPHFKGESGTMNVLQVMAEITILTAARTLQGEEVRSKLDSSFANYYHDLDSGFTPINFLLPWAPLPHNRKRDAA

HKKMRDVYMDIIKKRRISGQERGHDMLWNLMDNCVYKDGTALPDKEIAHLMITLLMAGQHTSSAISAWAILQLAAHPEVM

EGLYQEQVQVFGPSKSITLGDIDRLSFSQMVIKETLRLHAPIHSIMRKAKNTMAVPGTNLVIPESHILLSAPGFTARSED

HFKDAAKWNPHRWLSVIERSEADEKIDYGYGVTIKGTKSPYLPFGAGRHRCIGEKFAYVNLGVILAVMVRHFKLRNPDNR

VGVPATDYRSLLSRPMEPSSVHWEKRNFVSE

 

>CYP52A1 Candida tropicalis M24894

 

MSSSPSIAQEFLATITPYVEYCQENYTKWYYFIPLVILSLNLIS

MLHTKYLERKFKAKPLAVYVQDYTFCLITPLVLIYYKSKGTVMQFACDLWDKNLIVSD

PKAKTIGLKILGIPLIETKDPENVKAILATQFNDFSLGTRHDFLYSLLGDGIFTLDGA

GWKHSRTMLRPQFAREQVSHVKLLEPHMQVLFKHIRKHHGQTFDIQELFFRLTVDSAT

EFLLGESAESLRDESVGLTPTTKDFDGRNEFADAFNYSQTNQAYRFLLQQMYWILNGS

EFRKSIAIVHKFADHYVQKALELTDEDLEKKEGYVFLFELAKQTRDPKVLRDQLLNIL

VAGRDTTAGLLSFLFFELSRNPEIFAKLREEIENKFGLGQDARVEEISFETLKSCEYL

KAVINETLRIYPSVPHNFRVATRNTTLPRGGGEGGLSPIAIKKGQVVMYTILATHRDK

DIYGEDAYVFRPERWFEPETRKLGWAYVPFNGGPRICLGQQFALTEASYVTVRLLQEF

GNLKQDPNTEYPPKLQNTLTLSLFEGAEVQMYLIL

 

>CYP52A2 C. tropicalis X17560

 

ISLLNILVAGRDTTAGLLSFVFFELARTPRVANKLREEIEDKFG

LGQDARVEEISFESLKSCEYLKAVLNECLRLYPSVPQNFRVATRNTTLPRGGGKDGLS

PVLVRKGQTVMYSVYAAHRNKQIYGEDALEFRPERWFEPETKKLGWAFLPFNGGPRIC

LGQQFALTEASYVTVRLLQEFSHLTMDPNTEYSPKKMSHLTMSLFDGANIQMY

 

>CYP52A3 Candida maltosa P450cm1. X51931

 

MAIEQIIEEVLPYLTKWYTIIFGAAVTYFLSIALRNKFYEYKLK

CENPVYFQDAGLFGIPALIDIIKVRKAGQLADYTDTTFDKYPNLSSYMTVAGVLKIVF

TVDPENIKAVLATQFNDFALGARHAHFDPLLGDGIFTLDGEGWKHSRAMLRPQFAREQ

IAHVKALEPHVQILAKQIKLNKGKTFDLQELFFRFTVDTATEFLFGESVHSLYDEKLG

IPAPNDIPGRENFAEAFNTSQHYLATRTYSQIFYWLTNPKEFRDCNAKVHKLAQYFVN

TALNATEKEVEEKSKGGYVFLYELVKQTRDPKVLQDQLLNIMVAGRDTTAGLLSFAMF

ELARNPKIWNKLREEVEVNFGLGDEARVDEISFETLKKCEYLKAVLNETLRMYPSVPI

NFRTATRDTTLPRGGGKDGNSPIFVPKGSSVVYSVYKTHRLKQFYGEDAYEFRPERWF

EPSTRKLGWAYLPFNGGPRICLGQQFALTEASYVIARLAQMFEHLESKDETYPPNKCI

HLTMNHNEGVFISAK

 

>CYP52A4 Candida maltosa ALK3-A X55281

 

MPVSFVHNVLEVVTPYVEYYQENLTKWYILIPTILLTLNFLSIL

HTKYLEYKFNAKPLTNFAQDYSFGVITPLMLMYFKWHGTVMEFACNVWNNKFLVLNGN

VRTVGLRIMGLNIIETTDPENVKAILATQFNDFSLGTRHDFLYSLLGDGIFTLDGAGW

KHSRAMLRPQFAREQVAHVKLLEPHVQVLFKHVRKSQGKTFDIQELFFRLTVDSSTEF

LFGGSVESLRDASIGMTPSTKNIAGREEFADAFNYSQTYNAYRFLLQQFYWILNGSKF

NKSIKTVHKFADFYVQKALSLTEADLEKQEGYVFLYELAKQTRDPKVLRDQLLNILVA

GRDTTAGLLSFLFFELSRNPTVFEKLKEEIHNRFGAKEDARVEEITFESLKLCEYLKA

CVNEALRVYPSVPHNFRVATRNTTLPRGGGKDGMSPIAIKKGQNVMYTILATHRDPNI

YGEDANVFRPERWFEPETRKLGWAYVPFNGGPRICLGQQFALTEASYVTVRLLQEFHT

LTQDADTRYPPRLQNSLTLSLCDGANIQMY

 

>CYP52A5 Candida maltosa ALK2-A X55281

 

MTSDSTIHELIQSYITKWYVIVPLAIIIYKVFDYFYVLSLRKRL

GAAVPTNEETDGYFGFHLPFVLMSKKKDGTIIDFSIERYPELKHPETPTFEFPIFTVK

LISTIDPENIKAILATQFSDFSLGTRHAHFAPLIGDGIFTLDGAGWKHSRAMLRPQFA

REQVGHVKLLEPHVQVLFKHIRKNKGREFDLQELFFRFTVDSATEFLFGESVESLRDA

SIGMTSKSKDVDGIEDFTGAFNYSQNYLASRSIMQQFYWILNGKKFRECNAIVHKFAD

HYVQKALNLTEADLEKQAGYVFLYELVKQTRDPQVLRDQLLNILVAGRDTTAGLLSFV

FFELARNPDVVAKLKDEIDTKFGLGEDARIEEITFESLKQCEYLKAVLNECLRLYPSV

PQNFRVATKNTTLPRGGGKDGLSPILVRKGQTVMYSVYATHRMESVYGKDATTFRPER

WFEPETRKLGWAFVPFNGGPRICLGQQFALTEASYVTVRLLQEFSTLTLDPNLEYPPK

KMSHLTMSLFDGTNVQMY

 

>CYP52A6 C.tropicalis alk3 Z13010

 

MATQEIIDSALPYLTKWYTVITLAALVFLISSNIKNYVKAKKLK

CRDPPYFKGAGWTGISPLIEIIKVKGNGRLARFWPIKTFDDYPNHTFYMSIIGALKIV

LTVIQENIKAVLATQFTDFSLGTRHAHFYPLLGDGIFTLDGEGWKHSRAMLRPQFARD

QIGHVKALEPHIQILAKQIKLNKGKTFDIQELFFRFTVDTATEFLFGESVHSLYDEKL

GIPTPNEIPGRDNFATAFNTSQHYLATRTYSQTFYFLTNPKEFRDCNAKVHYLAKYFV

NKALNFTPEEIEEKSKSGYVFLYELVKQTRDPKVLQDQLLNIMVAGRDTTAGLLSFAM

FELARHPEIWSKLREEIEVNFGVGEESRVEEITFESLKRCEYLKAILNETLRMYPSVP

VNSRTATRDTTLPRGGGPNGTDPIFIPKGSTVAYIVYKTHRLEEYYGKDADDFRPERW

FEPSTKKLGWAYVPFNGGPRICLGQQFALTEASYVITRLVQMFETVSSPPDVEYPPPK

CIHLTMSHDDGVFVKM

 

>CYP52A7 C.tropicalis alk4 Z13011

 

MIEQVLHYWYYVLPAFIIFHWIVSAIHTNSLRRKLGAKPFTHTQ

LDGFYGFKFGRDFLKAKRIGRQVDLINSRFPDDIDTFSSYTFGNHVIFTRDPENIKAL

LATQFNDFSLGGRIKFFKPLLGYGIFTLDGEGWKHSRAMLRPQFAREQLPMSPSLEPH

FNVKAYPQEQRWVFDIQELFFRFTVDSATEFLFGESVNSLKSASIGCDEETELEERKK

FAEAFNKAQEYISTRVALQQLYWFVNNSEFKECNEIVHKFTNYYVQKALDATPEELEK

QSGYVFLYELVKQTRDPNVLRDHHSISLLAGRDTTAGLLSFAVFELARNPHIWAKLRE

DVESQFGLGEESRIEEITFESLKRCEYLKAVMNETLRLHPSVPRNARFALKDTTLPRG

GGPDGKDPILVRKMSCSIFISGTQIDPKHYGKDAKLFRPERWFESSTRNLGWAYLPFN

GGPRICLGQQFALTEAGYILVRLAQSFDTLELKPDTEYLTKISHLTMCLFGAFVKMD

 

>CYP52A8 C.tropicalis alk5 Z13012

 

MYEQVVEYWYVVLPLVFILHKVFDMWHTRRLMKQLGAAPVTNQL

HDNFFGIINGWKHLSSRKKVELKNIMIINLPIRKFQVWVHMLVPSLEQSSSSQKIRRN

IKALLATQFSDFSLGKRHTLFKPLLGDGIFTLDGQGWKHSRAMLRPQFAREQVAHVTS

LEPHFQLLKKHMVKNKGGFFDIQELFFRFTVDSATEFLFGESVHSLKDETIGSYQDDI

DFVGRKDFAESFNKAQEYLAIRTLVQDFYYLVNNQEFRDCNKLVHKFTNYYVQRALDA

TPEELEKQSGYVFLYELVKQTRGPNVLRDQSLNILLAGRDTSAGLLSFAVFELARNPH

IWAKLREDVESQFGLGEQSRIEEITFESLKRCGYLKAFLNETLRVYPSVPRNFRIATK

NTTLPRGGGSDGNSPVLVKKGEAVSYGINSTHLDPVYYGDDAAEFRPERWNEPSTRKL

GWAYLPFNGGPRICLGQQFALTEAGYVLVRLAQSFDTLELKPPVVYPPKRLTNLTMSL

QDGTIVKID

 

>CYP52A9 Candida maltosa D12717

 

MIDEILPKLVQYWYIVLPTLLIIKHVVSYINTQRLMRKFRAKPV

TNVLNDGFFGIPNGIKAIKEKNKGRAQEYNDEKFAAGPKPKVGTYLFKLFTKDVLVTK

DPENIKAILATQFEDFSLGKRLDFFKPLLGYGIFTLDGEGWKHSRAMLRPQFAREQVG

HVKLIEPHFQSLKKHIIKNKGQFFDIQELFFRFTVDSATEFLFGESVESLKDESIGYD

QQDFDFDGRKNFAEAFNKAQEYLGTRAILQLFYWLVNGADFKKSVAEVHKFTDYYVQK

ALDATPEELEKHSGYIFLYELVQQTRDPKVLRDQSLNILLAGRDTTAGLLSFALFELA

RNPEVWSRLREEIGDKFGLDEDATIEGISFESLKQCEYLKAVVNECLRMYPSVPRNFR

IATKHTTLPRGGGPDGKDPIFIKKGAVVSYGINSTHLDPMYYGPDARLFNPDRWSKPE

TKKLGWAFLPFNGGPRICLGQQFALTEASYVLVRMIQNFKELELTPNTVYPPRRLTNL

TMSLYDGAYIKVN

 

>CYP52A10 Candida maltosa D12719

 

MIFTAFILDYWYFTLLFLIAAYFIGKHVYTNYLMRKHHAEPILD

VVDDGAFGFKFGFQSLKAKKIGNQIDLLFLKFNEAKHPSIGTFVTRSFGMQFIATKDP

ENIKAMLATQFNEYTLGQRLNFLAPLLGKGIFTLDGNGWKHSRAMLRPQFSRDQIGHV

KMLEPHFQLLKKHIIKNKGTFFDIQELFFRFTVDSATEFLFGESVSSLKDESIGYDQE

EIDFAGRKDFAEAFNKSQVYLSTRTLLQLLYWLVNSADFKRCNKIVHKFSDYYIKKAL

TATPEELEKHSSYIFLYELAKQTRDPIVLRDQSLNILLAGRDTTAGLLSFAVFELGRN

PEVWSKLRQEIGHKFGLDSYSRVEDISFELLKLCEYLKAVLNETLRLYPSVPRNARFA

AKNTTLPHGGGPDGMSPILVRKGQTVMYSVYALQRDEKYYGKDANEFRPERWFEPEVR

KLGWAFLPFNGGPRICLGQQFALTEASYVLARLIQSFETLELSPEAAYPPAKLSHLTM

CLFDGTPVRFE

 

>CYP52A11 Candida maltosa D12719

 

MVFTAFILEYWYFTLLSLAAGHFIGKHVYTNYLMRKHHAEPILD

VVDDGAFGFKFGFQALKAKKIGKQIDLLFKKFNEAKHPSIGTFVTRSFGMQFIATKDP

ENIKAMLATQFNDFTLGQRLSYFAPLLGKGIFTLDGEGWKHSRAMLRPQFSRDQVGHV

KMLEPHFQLLKKHIIKNKGSFFDIQELFFRFTVDSATEFLFGESVSSLKDESIGYDQE

EIDFAGRKDFAEAFNKSQVYLSTRSLLQLLYWLVNSSDFKRCNKIVHKFSDYYIKKAL

TATPEELEKHSSYIFLYELAKQTRDPIVLRDQSLNILLAGRDTTAGLLSFAVFELGRN

PEVWSKLREEIGDKFGLDPDSRIEDISFELLKLCEYLKAVINETLRLYPSVPRNGRFA

AANTTLPHGGGPDGMSPILVRKGQTVMYSVYALQRDEKYYGKDANEFRPERWFEPEVR

KLGWAFLPFNGGPRICLGQQFALTEASYVLVRLIQSFETLELSPDAPYPPAKLTHLTM

CLFDGAPVRIE

 

>CYP52A12 Candida tropicalis

AY230498

MATQEIIDSVLPYLTKWYTVITAAVLVFLISTNIKNYVKAKKLK

CVDPPYLKDAGLTGISSLIAAIKAKNDGRLANFADEVFDEYPNHTFYLSVAGALKIVM

TVDPENIKAVLATQFTDFSLGTRHAHFAPLLGDGIFTLDGEGWKHSRAMLRPQFARDQ

IGHVKALEPHIQIMAKQIKLNQGKTFDIQELFFRFTVDTATEFLFGESVHSLYDEKLG

IPTPNEIPGRENFAAAFNVSQHYLATRSYSQTFYFLTNPKEFRDCNAKVHHLAKYFVN

KALNFTPEELEEKSKSGYVFLYELVKQTRDPKVLQDQLLNIMVAGRDTTAGLLSFALF

ELARHPEMWSKLREEIEVNFGVGEDSRVEEITFEALKRCEYLKAILNETLRMYPSVPV

NFRTATRDTTLPRGGGANGTDPIYIPKGSTVAYVVYKTHRLEEYYGKDANDFRPERWF

EPSTKKLGWAYVPFNGGPRVCLGQQFALTEASYVITRLAQMFETVSSDPGLEYPPPKC

IHLTMSHNDGVFVKM

 

>CYP52A13 Candida tropicalis

AY230499

MTVHDIIATYFTKWYVIVPLALIAYRVLDYFYGRYLMYKLGAKP

FFQKQTDGCFGFKAPLELLKKKSDGTLIDFTLQRIHDLDRPDIPTFTFPVFSINLVNT

LEPENIKAILATQFNDFSLGTRHSHFAPLLGDGIFTLDGAGWKHSRSMLRPQFAREQI

SHVKLLEPHVQVFFKHVRKAQGKTFDIQELFFRLTVDSATEFLFGESVESLRDESIGM

SINALDFDGKAGFADAFNYSQNYLASRAVMQQLYWVLNGKKFKECNAKVHKFADYYVN

KALDLTPEQLEKQDGYVFLYELVKQTRDKQVLRDQLLNIMVAGRDTTAGLLSFVFFEL

ARNPEVTNKLREEIEDKFGLGENASVEDISFESLKSCEYLKAVLNETLRLYPSVPQNF

RVATKNTTLPRGGGKDGLSPVLVRKGQTVIYGVYAAHRNPAVYGKDALEFRPERWFEP

ETKKLGWAFLPFNGGPRICLGQQFALTEASYVTVRLLQEFAHLSMDPDTEYPPKKMSH

LTMSLFDGANIEMY

 

>CYP52A14 Candida tropicalis

AY230500

MTAQDIIATYITKWYVIVPLALIAYRVLDYFYGRYLMYKLGAKP

FFQKQTDGYFGFKAPLELLKKKSDGTLIDFTLERIQALNRPDIPTFTFPIFSINLIST

LEPENIKAILATQFNDFSLGTRHSHFAPLLGDGIFTLDGAGWKHSRSMLRPQFAREQI

SHVKLLEPHMQVFFKHVRKAQGKTFDIQELFFRLTVDSATEFLFGESVESLRDESIGM

SINALDFDGKAGFADAFNYSQNYLASRAVMQQLYWVLNGKKFKECNAKVHKFADYYVS

KALDLTPEQLEKQDGYVFLYELVKQTRDRQVLRDQLLNIMVAGRDTTAGLLSFVFFEL

ARNPEVTNKLREEIEDKFGLGENARVEDISFESLKSCEYLKAVLNETLRLYPSVPQNF

RVATKNTTLPRGGGKDGLSPVLVRKGQTVMYGVYAAHRNPAVYGKDALEFRPERWFEP

ETKKLGWAFLPFNGGPRICLGQQFALTEASYVTVRLLQEFGHLSMDPNTEYPPRKMSH

LTMSLFDGANIEMY

 

>CYP52A15 Candida tropicalis

AY230501

MSSSPSFAQEVLATTSPYIEYFLDNYTRWYYFIPLVLLSLNFIS

LLHTRYLERRFHAKPLGNFVRDPTFGIATPLLLIYLKSKGTVMKFAWGLWNNKYIVRD

PKYKTTGLRIVGLPLIETMDPENIKAVLATQFNDFSLGTRHDFLYSLLGDGIFTLDGA

GWKHSRTMLRPQFAREQVSHVKLLEPHVQVFFKHVRKHRGQTFDIQELFFRLTVDSAT

EFLFGESAESLRDESIGLTPTTKDFDGRRDFADAFNYSQTYQAYRFLLQQMYWILNGS

EFRKSIAVVHKFADHYVQKALELTDDDLQKQDGYVFLYELAKQTRDPKVLRDQLLNIL

VAGRDTTAGLLSFVFYELSRNPEVFAKLREEVENRFGLGEEARVEEISFESLKSCEYL

KAVINETLRLYPSVPHNFRVATRNTTLPRGGGEDGYSPIVVKKGQVVMYTVIATHRDP

SIYGADADVFRPERWFEPETRKLGWAYVPFNGGPRICLGQQFALTEASYVTVRLLQEF

AHLSMDPDTEYPPKLQNTLTLSLFDGADVRMY

 

>CYP52A16 Candida tropicalis

AY230502

MSSSPSFAQEVLATTSPYIEYFLDNYTRWYYFIPLVLLSLNFIS

LLHTKYLERRFHAKPLGNVVLDPTFGIATPLILIYLKSKGTVMKFAWSFWNNKYIVKD

PKYKTTGLRIVGLPLIETIDPENIKAVLATQFNDFSLGTRHDFLYSLLGDGIFTLDGA

GWKHSRTMLRPQFAREQVSHVKLLEPHVQVFFKHVRKHRGQTFDIQELFFRLTVDSAT

EFLFGESAESLRDDSVGLTPTTKDFEGRGDFADAFNYSQTYQAYRFLLQQMYWILNGA

EFRKSIAIVHKFADHYVQKALELTDDDLQKQDGYVFLYELAKQTRDPKVLRDQLLNIL

VAGRDTTAGLLSFVFYELSRNPEVFAKLREEVENRFGLGEEARVEEISFESLKSCEYL

KAVINEALRLYPSVPHNFRVATRNTTLPRGGGKDGCSPIVVKKGQVVMYTVIGTHRDP

SIYGADADVFRPERWFEPETRKLGWAYVPFNGGPRICLGQQFALTEASYVTVRLLQEF

GNLSSDPNAEYPPKLQNTLTLSLFDGADVRMF

 

>CYP52A17 Candida tropicalis

AY230504

MIEQLLEYWYVVVPVLYIIKQLLAYTKTRVLMKKLGAAPVTNKL

YDNAFGIVNGWKALQFKKEGRAQEYNDYKFDHSKNPSVGTYVSILFGTRIVVTKDPEN

IKAILATQFGDFSLGKRHTLFKPLLGDGIFTLDGEGWKHSRAMLRPQFAREQVAHVTS

LEPHFQLLKKHILKHKGEYFDIQELFFRFTVDSATEFLFGESVHSLKDESIGINQDDI

DFAGRKDFAESFNKAQEYLAIRTLVQTFYWLVNNKEFRDCTKSVHKFTNYYVQKALDA

SPEELEKQSGYVFLYELVKQTRDPNVLRDQSLNILLAGRDTTAGLLSFAVFELARHPE

IWAKLREEIEQQFGLGEDSRVEEITFESLKRCEYLKAFLNETLRIYPSVPRNFRIATK

NTTLPRGGGSDGTSPILIQKGEAVSYGINSTHLDPVYYGPDAAEFRPERWFEPSTKKL

GWAYLPFNGGPRICLGQQFALTEAGYVLVRLVQEFSHVRSDPDEVYPPKRLTNLTMCL

QDGAIVKFD

 

>CYP52A18 Candida tropicalis

AY230505

MIEQILEYWYIVVPVLYIIKQLIAYSKTRVLMKQLGAAPITNQL

YDNVFGIVNGWKALQFKKEGRAQEYNDHKFDSSKNPSVGTYVSILFGTKIVVTKDPEN

IKAILATQFGDFSLGKRHALFKPLLGDGIFTLDGEGWKHSRSMLRPQFAREQVAHVTS

LEPHFQLLKKHILKHKGEYFDIQELFFRFTVDSATEFLFGESVHSLKDETIGINQDDI

DFAGRKDFAESFNKAQEYLSIRILVQTFYWLINNKEFRDCTKSVHKFTNYYVQKALDA

TPEELEKQGGYVFLYELVKQTRDPKVLRDQSLNILLAGRDTTAGLLSFAVFELARNPH

IWAKLREEIEQQFGLGEDSRVEEITFESLKRCEYLKAFLNETLRVYPSVPRNFRIATK

NTTLPRGGGPDGTQPILIQKGEGVSYGINSTHLDPVYYGPDAAEFRPERWFEPSTRKL

GWAYLPFNGGPRICLGQQFALTEAGYVLVRLVQEFSHIRSDPDEVYPPKRLTNLTMCL

QDGAIVKFD

 

>CYP52A19 Candida tropicalis

AY230506

MLDQILHYWYIVLPLLAIINQIVAHVRTNYLMKKLGAKPFTHVQ

RDGWLGFKFGREFLKAKSAGRSVDLIISRFHDNEDTFSSYAFGNHVVFTRDPENIKAL

LATQFGDFSLGSRVKFFKPLLGYGIFTLDAEGWKHSRAMLRPQFAREQVAHVTSLEPH

FQLLKKHILKHKGEYFDIQELFFRFTVDSATEFLFGESVHSLKDEEIGYDTKDMSEER

RRFADAFNKSQVYVATRVALQNLYWLVNNKEFKECNDIVHKFTNYYVQKALDATPEEL

EKQGGYVFLYELVKQTRDPKVLRDQSLNILLAGRDTTAGLLSFAVFELARNPHIWAKL

REEIEQQFGLGEDSRVEEITFESLKRCEYLKAVLNETLRLHPSVPRNARFAIKDTTLP

RGGGPNGKDPILIRKDEVVQYSISATQTNPAYYGADAADFRPERWFEPSTRNLGWAFL

PFNGGPRICLGQQFALTEAGYVLVRLVQEFPNLSQDPETKYPPPRLAHLTMCLFDGAH

VKMS

 

>CYP52A20 Candida tropicalis

AY230507

MLDQIFHYWYIVLPLLVIIKQIVAHARTNYLMKKLGAKPFTHVQ

LDGWFGFKFGREFLKAKSAGRQVDLIISRFHDNEDTFSSYAFGNHVVFTRDPENIKAL

LATQFGDFSLGSRVKFFKPLLGYGIFTLDGEGWKHSRAMLRPQFAREQVAHVTSLEPH

FQLLKKHILKHKGEYFDIQELFFRFTVDSATEFLFGESVHSLRDEEIGYDTKDMAEER

RKFADAFNKSQVYLSTRVALQTLYWLVNNKEFKECNDIVHKFTNYYVQKALDATPEEL

EKQGGYVFLYELAKQTKDPNVLRDQSLNILLAGRDTTAGLLSFAVFELARNPHIWAKL

REEIESHFGSGEDSRVEEITFESLKRCEYLKAVLNETLRLHPSVPRNARFAIKDTTLP

RGGGPNGKDPILIRKNEVVQYSISATQTNPAYYGADAADFRPERWFEPSTRNLGWAYL

PFNGGPRICLGQQFALTEAGYVLVRLVQEFPSLSQDPETEYPPPRLAHLTMCLFDGAY

VKMQ

 

>CYP52A21 Candida albicans SC5314

putative P450 drug resistance protein

XM_705082, orf19_10

MLEEISFESWYLYLIICGVLYFCTNYLYIQYLNKKLGAKSPGSC

KSDGCFGFRSGFEMLKAKKIGRQVDLLHERFNDLSNPEVNDTFTSYNFGFPMIITKDP

ENIKALLATQFNDFSLGRRYDFFKPLLGKGIFTLDGEGWKHSRSMLRPQFAREQIAHV

KALEPHFQLLKKHITKNKGQFFDIQELFFRFTVDSATEFLFGESVSSLKDESIGYNQD

EFDFAGRKDFPEAFNKSQIYLSTRSLLQNLYWLVNPKDFQNCNAIVHKFSDYYVNKAL

NATPEELEKNSGYVFLYELVKQTRDPQVLRDQALNILLAGRDTTAGLLSFAVFELARN

PQVWKKLREEIIERYADGATEISFETLKQCEYLKAVINETLRLYPSVPRNGRFANKNT

TLPRGGGPDGMSPILIKQGQAVMYSIYSTQRDPKYYGKDADVFRPERWFEPETRKLGW

AFLPFNGGPRICLGQQFALTEASYVLVRLAQCCENLQLTEGIEYPPAKLTHLTMCMFD

GVPIKVD

 

>CYP52A22 Candida albicans SC5314

79% to CYP52A4 orf19_7512

MSPSLVHQILAITHPYVEYLEENITKWYILVPGILIVLNVLTTLHTKYLEYKFNAKPVTNFIQDYTFGVITPLILIYYKS

QGTVMEFANNFWNFKFLIKNPDVGTGELRIFGMHLIETKDPENIKAVLATQFNDFSLGTRHGFLYSLLGDGIFTLDGAGW

KHSRSMLRPQFAREQIAHVKILEPHMQVFFKHIRKNKGKTFDIQELFFRLTVDSSTEFLFGSSVESLRDETIGMSPSVKN

LAGRDEFADAFNYSQTINAYRFLLQQFYWLCNGTKFRKSIAAVHKFSDFYVQKALSLSQDELDEQKGYVFLYELAKQTRD

PKVLRDQLLNILVAGRDTTAGLLSFVFFELARNPEVYAKLKEEIYNKFGSGEDARIDEITFESLKQCEYLKAVINESLRL

YPSVPHNFRTATRNTTLPRGGGPDGMSPIVVKKGQSVMYTVLVTHRDTKTYGADANEFRPERWFEPETRKLGWAYVPFNG

GPRICLGQQFALTEASYVTVRLLQEFEHLTMDAETRYPPKLMNSLTLSLLDGANVEMY

 

>CYP52A23 Candida albicans SC5314

78% to CYP52A5 orf19_7513

MSVSDIVQEYITKWYVVIPTLFVVYQVVDFFYVRSLRSRLGAKAPTNNESDGYWGFHLPFLLVSKKAEGTIIDFAGERFK

SIAHPEVPTFQFPIFTVKVISTVDPENIKAVLATQFNDFSLGTRHNQFAPLLGDGIFTLDGAGWKHSRSMLRPQFAREQV

AHVKALEPHMQVLFKHIRKSGGRTFDLQELFFRFTVDSATEFLFGESVESLRDESIGMALEAVDFDGKKEFAEAFNFSQN

YLASRAVMQQMYWLLNSSEFKSCNSKVHKFAEYYVNKALELSHEELEKQQGYVFLYELAKQTRDTKVLRDQLLNILVAGR

DTTAGLLSFVFFELARNPRVFAKLREEVEEKFGVGEDARVEEISFESLKSCEYLKAVLNECLRLYPSVPQNFRVATKNTT

LPRGGGSDGMSPILVRKGQTVMYSVYVTHRDTKVYGKDADEFRPERWFEPETRKLGWSFVPFNGGPRICLGQQFALTEAS

YVTVRLLQEFGHLTMDPNTDYPPKKMSHLTMSLYDGTNVEMY

 

>CYP52A24 Candida albicans SC5314

78% to CYP52A3 orf19_5728 partial sequence

MSSTTFNDIIDSVLPYLTKWYTILIGAGLIFVLSTNLKNAYYSYKLGCKNPVHLKRAGWTGIPALIDAIKAKNSGKLAEF

GGDTFDELNSKNFYLNVAGVLKIIFTIEPENIKAVLATQFNDFSLGTRHAHFYPLLGDGIFTLDGEGWKHSRAMLRPQFA

REQIAHVKSLEPHIQILAKQIKLNKGETFDLQELFFRFTVDTATEFLFGESVHSLYDDKLGIPPPNDIPGRDNFATAFNT

SQHYLATRSYSQTFYWLTNNKEFRDCNAKVHHLAKYFVDKALNSTEKEIEEKSQGGYVFLYELVKQTKNPKVLQDQLLNI

MVAGRDTTAGLLSFAMFELARNPKVWNKLREELEANYGVGEEARVEDITFENLKKM

 

>CYP52A25 Candida dublinensis

79% to CYP52A3   Candida maltosa

cdub_593-g99.1

MSSATTSSNIIDSVLPYLTKWYTILIGAGLIFILSTNLKNAYYSYKLGCKNPVYLKRAGWTGIPALIDAIKAKNSGKLAE

FGDDTFEELNTKTFYLNVAGVLKIIFTIDPENIKAVLATQFNDFSLGTRHAHFYPLLGDGIFTLDGEGWKHSRAMLRPQF

AREQIAHVKSLEPHIQILAKQIKLNKGETFDLQELFFRFTVDTATEFLFGESVHSLYDDKLGIPPPNDIPGRDNFATAFN

ISQHYLATRSYSQTFYWLTNNKEFRDCNAKVHYLAKYFVDKALNSTEEEIEEKSQGGYVFLYELVKQTKNPKVLQDQLLN

IMVAGRDTTAGLLSFAMFELARNPKVWNKLREEIETNFGVGEESRVEDITFENLKKCEYLKAILNETLRLYPSVPINFRT

STKDTTLPTGGGRDGTSPIFVPKGSTVAYTVYKTHRLEEYYGKDSYEFRPERWFEPLTKKLGWAYLPFNGGPRICLGQQF

ALTEASYVITRLAQLFPVLETREKQYPPNKCIHLTMNHDEGVFVSMN

 

>CYP52A26 Candida dublinensis

89% to CYP52A21   Candida albicans

cdub_14-g136.1

MLEEISFESWYIYLFICGVLYFGSKYFYTQYLNNKFGAKLPGSFRSDGLFGFKNGFEMLKAKKVGRQVDLVHSRFNDLSK

THVNDTFKEYNFGIPVIVTKDPENIKALLATQFNDFSLGRRSDFFYPLLGKGIFTLDGEGWKHSRAMLRPQFAREQIAHV

KALEPHFQLLKKHITKNKGKGFDIQELFFRFTVDSATEFLFGESVSSLKDESIGYNQDEFDFDGRKDFPEAFNKAQIYLA

TRAILKDLYWLVNPKDFQNCNAIVHKFSDYYVNKALNATPEELEKNSGYVFLYELVKQTRDPQVLRDQALNILLAGRDTT

AGLLSFAMFELARNPQIWQKLREEVIEKYADEVTEIAFETLKQCEYLKAVINETLRLYPSVPRNGRFANKNTTLPRGGGP

DGMSPILIKQGQGVLYSIYSTQRDTKFYGKDADVFRPERWFEPETRKLGWAFLPFNGGPRICLGQQFALTEASYVLVRLA

QSYENLQLTENIEYPPPKLTHLTMCMFDGVPIKIA

 

>CYP52A27 Candida dublinensis

94% to CYP52A23   Candida albicans

cdub_14-g720.1

MTVSTLEIQDIIQQYITKWYVIIPTLFVVYQVVDFLYVRSLRSRFGAKAPTNNESDGYWGFHLPFLLVSKKAEGTIIDFA

GERFKNITHPEVPTFQFPIFTVKVISTVDPENIKAVLATQFNDFSLGTRHNQFAPLLGDGIFTLDGAGWKHSRSMLRPQF

AREQVAHVKALEPHMQVFFKHVRKSGGRTFDIQELFFRLTVDSATEFLFGESVESLRDESIGMALEAVDFDGKKEFAQAF

NFSQNYLASRAVMQQMYWLLNSSEFQSCNTKVHKFAEYYVNKALELSPESLEKQQGYVFLYELVKQTRDTKVLRDQLLNI

LVAGRDTTAGLLSFVFFELARNPRVFAKLREEIEQKFGVGEDSRVEEISFESLKLCEYLKAVLNECLRLYPSVPQNFRVA

TKNTTLPRGGGPDGLSPILVRQGQTVMYSVYVTHRDTKVYGKDANEFRPERWFEPETRKLGWSFVPFNGGPRICLGQQFA

LTEASYVTVRLLQEFGHLTMDPNTQYPPKKMSHLTMSLFDGTNVEMY

 

>CYP52A28 Candida dublinensis

93% to CYP52A22   Candida albicans

cdub_14-g719.1

MSPSLVHQILAIAHPYVEYLQANFTKWYILVPGILIALNVLTFLYTKYLEYKFNAKPVTNFIQDYTFGVLTPLILIYYKS

QGTVMEFANNLWGNRFFIKNADVGTGELRIFGMHLIETKDPENIKAILATQFNDFSLGTRHGFLYSLLGDGIFTLDGAGW

KHSRSMLRPQFAREQVSHVKILEPHMQVFFKHVRKNKGKAFDIQELFFRLTVDSSTEFLFGSSVESLRDETIGMSPSVKN

LAGRDEFAEAFNYSQTINAYRFLLQQFYWLCNGSKFRKSIATVHKFSDFYVQRALSLSKAELDDQKGYVFLYELAKQTRD

PKVLRDQLLNILVAGRDTTAGLLSFVFFELARNPTVYAKLKEEIYNKFGSGEDARIDEITFESLKQCEYLKAVINESLRL

YPSVPHNFRTATRNTTLPRGGGPDGMSPIVVKKGQSVMYTVLATHRDTKTYGADANDFRPERWFEPETRKLGWAYVPFNG

GPRICLGQQFALTEASYVTVRLLQEFGHLTMDPETCYPPKLMNSLTLSLLEGANVEMY

 

>CYP52A29 Candida lusitaniae

55% to CYP52A13   Candida tropicalis

CLUG_03984.1

MSLHGVFGGMSIWSSVLLFIGIYLAGSYIRRYIAYKRSGAMWPAYGTDKCLGFKVVREMLKMRQIGRAPDFFQSKFEITG

VYTGGLLVGGRYAITTRDPENVKAILATQFNDFNLGNRNQHIKVTLGDGIFTLDGAGWKSSRAMLRPQFAREQIAHVQSL

ERHVQRLAQKVRGTKGERFDIQPLFAKLTIDSGTEFLFGESCESLVDGESESETGLDPALKAVFAEAFNYTQTILFQRLS

LQKLYFLVDGFKFRRQNRIVHQVTDFFVERALHASDKEAHSSSRGGYVFLYELMKQTRDPKVLRDQCLNVLLAARDTTSG

LLSFCFFELARNPAVFARLREEISFHFGLGESADLSAISFESLKKCEYLRAVLHETLRMYPSVPANFRVATRDTTLPHGG

GPDESKPIFVPKGTMCIYQVYSVHRSEVHYGKDYNDFRPERWFEERTKKLGWAFLPFNGGPRICLGQQFALTEASYVIVR

LLQMFEHIESFDSVYPPAKATHLTMSLFDGANIALW

 

>CYP52A30 Candida lusitaniae

55% to CYP52A23   Candida albicans

CLUG_04851.1

MLLSELKTAWDSFSSHLSDNVFLYYAELLILTLVVYHTYRTSKKWYLRKKLGAESAKWLQTSSAGWINSLPMREILDAKR

EGYLLRELWKYWHDDDENFMVKVAGTEMFVTTDPENYKALLATQFNDFKLGIRHSHFKPLLGDGIFTLDYGGWKHSRALL

RPNFSREKVGHLHSLETHLQVLFKHIRKHNGKPFDLQELFFRLTVDTATEFLFGHSANTLEDESIGIHKEETFEGEKEFY

DCFNISQAICATRAWTQKGYFLTIPFLYKEFTRCNRVVHNFADYYVNMALNMSQDELEKESNDGYIFLYELAKETRDPKV

LRDQLLNIMIAGRDTTAGLMSFIFFELAKRPDVWAKLKAEIYERFGYGDNVRFDEITFETLKKCTYLKNVVNETLRLYPS

VPVNYRQANINTTLPRGGGPDGDKPVLIEKGNAVGYIVSATHRNEKYYGKDAAEFKPERWDDKNLKPGWAYLPFNGGPRI

CLGQQFAITEASYVVTRMIQEFPNIADHDVEPDTYPPRMISQLTNSLAAGCYVSLTK

 

>CYP52A31 Candida lusitaniae

52% to CYP52A18   Candida tropicalis

CLUG_04098.1

MSLFRELDPVQLVYVVISLFFASFVYNELKGIYLSRKWHTKSPVKTYRGLFSLPQLVRSRKAVDSGHFVDYFRDQYIKYG

ADTLDFTGFGSKIFFTKNPHNVKSLFATNFDDWSIGARKRAFKPFLGVGVFVTDGEVWKHSRTMLKPQFSREQIAHVHSV

ESHYKLFAQHIRSYRGQEFDIQPLFMKLTMDASSEFLFGESIHLLHDDSIEHEKDTSDIKGKKELAEDLSFLQTYLMFRV

MLLDWFWLENSKKFSEAIKRVHAFTSQFVDRVLSLTPEEREANSKDGYTFLYELAKVTNDPIVMRDQILNVMLAGRSTTA

SLLCSVLLELSRNPEVYEKLKNEVYEQFGNGKTKGEEITFETLKKCTYLRWVLNEGLRMYPPVPSNLRQAVRDTTLPKGG

GPDGESPVLVRKGQYVALHIYSMHRSEEFYGKDALSFRPERWADLSKIGWAFMPFGSGPRICLGQQFALTEASYILVRMV

QDFPHIASHNKTYPPRTYSRSTMHYIDGIRISLY

 

>CYP52A32 Candida parapsilosis

71% to CYP52A5   Candida maltosa

CPAG_03577

MSDLTLFDYATKWYVLAPALIVLYAVYIQVYNAYLIKKLGAAKEANCEGDGLFGFRLPFWLIERKENGTVVDYVAERFDE

VSHPSIPTFSIRIFMVKLWMTKDPENIKALLATQFNDFCLGTRHAQFKPLLGDGIFTLDGQGWKESRQMLRPQFARDQIS

HVKMLEPHIQVLFKQIHKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASIGMQAVSNEVEGKEQFAKSFNFSQNY

LASRTTMQGLYWLLNSKKFRDNNAVVHKFAQYYVKRALSLTPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRD

TTAGLLSFVFFELARSPAVLTKLREEIGAKFGLDKEARVDEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATKNTTL

PRGGGPDGMSPIFVTKGQIVQYCVYATHRMEEFYGKNANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASY

VTTRLVQEFSSLWMDPNTQYPPHKMSHLTMSLYDGCNVKME

 

>CYP52A33 Candida parapsilosis

73% to CYP52A5   Candida maltosa

CPAG_04394

MSDFDIMEYSTKWYIIAPALIVFYVVYKKINEAYLMKKLGAVRETNPEGDGYLGFKLPFWLIERKKNGTLVDFSEQRFHE

ISHPEVPTLSMRIFTVKLWATKDPENIKALLATQFNDFSLGTRHPQFKPLLGDGIFTLDNQGWKDSRQMLRPQFAREQIS

HVKMLEPHIQVLFKHVRKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASVGMQADSNEIDGKEQFAESFNFSQNY

LASRAVMQGLYWLLNSKKFRDSNAVVHRFAQHYVKRALSLSPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRD

TTAGLLSFVFFELARNPAVLAKLREEIGTKFGLGKEARIEEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATRNTTL

PRGGGPDGMSPILITKGQTVNYSVYATHRSEEYYGKDANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASY

VTTRLVQEFSSLWMDPNTQYPPHKMSHLTMSLYDGCNVKME


>CYP52A34 Candida parapsilosis

71% to CYP52A5   Candida maltosa

CPAG_04395

MRGQCGLPFFFRKNTCNSTITLESCNGYLTRATLATDIKRWAILFSCSTSIQTCLFFNYYNEVSMLDFNIMGYTISLFVL

APALIVLYVVYKIITEVYLMKKLGASRETNVVSDGAFSLKLAFTLIASKNNGTLIDYVAEMYRGAPHPEVPTFSSRIFTV

KFYLTKDPENIKALLATQFNDFCLGTRHAQFQALLGDGIFTLDGKGWKDSRQMLRPQFAREQISHVKMLEPHIQVLFKHV

RKNKGQVFDLQELFFRFTVDSATEFLFGESVDSLKDASVGMQADSNEFDGKEQFPQSFNFSQSYLASRVVISFMYWVLNS

KQFRDSNAVVHRFAQHYVKRALSLSPEELEKQNGYVFLYELAKHTRDPKVLQDQLLNILVAGRDTTAGLLSFVFFELARN

PAVLAKLREEIGTKFGLGKEARIEEITFESLKNCEYLKAVLNECLRLYPSVPQNFRVATRNTTLPRGGGPDGMSPILVTK

NQEVHYSVYSTHRMEEYYGKDANEFRPERWFEPQTRKLGWAFVPFNGGPRICLGQQFALTEASYVTTRLVQEFSSLWMDP

NTQYPPHKMSHLTMSLYDGCNVKME

 

>CYP52A35 Candida parapsilosis

65% to CYP52A21   Candida albicans

CPAG_05481

MIDSAIATYWYITIPSVFLLYIIISYINEQYLMRKFKAKPFTNYISGGFFGFQEGIAALKHKKAGTAIERYRDLYEELPN

PDVPTYKSFVFGTPLVFTKDPENIKAILATQFNDFSLGIRHAHFDPLLGDGIFTLDHQGWKDSRAMLRPQFARDQIAHVK

ALEPHFQFLLRHIEKNNGQFFDIQELFFRFTVDSATEFLFGSSVSSLQDESIGCDTTELDFVGRKEFPEAFNKSQLILST

RALLQKMYWIYNPKEFKKCNAIVHQFSDYYINKVLSCTPEEIEKQSGYVFLYELVKQTRNPKVLRDQALNILVAGRDTTA

GLLSFVLFELARNPDIYKKLRDEILDKFGTSNLEEITFESLKKCEYLKAVLNEALRMYPSVPRNYRVSTRNTTLPRGGGS

DGTSPIFIPKGRSVIYNIAATHMDPRYYGKDVEEFRPERWFEESTKKLGWAYLPFNGGPRICLGQQFALTEASYVVARLA

QTISKLELKDGYTYPPKKMTHLTMCMFDGVYVKMEKDATAAAAKSG

 

>CYP52A36 Candida parapsilosis

65% to CYP52A21   Candida albicans

CPAG_05482

MIETVSIANIVAYWYITIPAVLLGHFVIGYIHEQVLIRKLGAKPFANNVNGGFFGSALGVKSIVAKGKGRAVEFYQNLYH

VSPHTEVPTIKTYIFGTPIVFTKDPENIKAILATQFQDFCLGNRYHFLKPLLGKGIFTLDHEGWKDSRAILRPQFARDQI

AHVKALEPHFQFLLRHIEKNNGQFFDIQELFFRFTVDSATEFLFGSSVSSLQDESIGCDTSALDFSGRLEFAEAFNVSQV

YLSTRALLQRIYWLCNNQEFKKCNKIVHQFSDYYINKVLNCTPEEIEKQSGYVFLYELVKQTRDPIVLRDQALNILLTGR

DTTAGLLSFVLFELARNPEMYAKLRHEILDKFGTSNLEDITFENLKKCEYLKAILNETLRMYPSVPRNARVATRNTTLPR

GGGSDGTSPVFIPKGKAVIYNIAATHMDPRYYGKDVEEFRPERWFEESTKKLGWAYLPFNGGPRICLGQQFALTEASYVV

ARLAQTISKLELKEGYEYPPKKMTHLTMSMFDGVYVKMEL

 

>CYP52A37 Candida parapsilosis

58% to CYP52A12   Candida tropicalis

CPAG_05523

MALDLASISSFITIHLFTWYGAIISFALIYATVGKIKREIFIRKHGCKEAYVFPDGGPLGLVLAFILQKYKSDGTILDFA

LSLYEDYGKRTTFTAYLFGVRVVVSGEPENMKAVLATQFNDYSLGSRHAHFAPLLGDGIFTLDNEGWKESRQLLRPQFAR

EQISHVKMLEPHVQVLAEHIRKHKGQTFDIQELFFRLTVDASTEFLFGESVHSLYDESIGRKCDPEVAGFADAFNAAQVA

LGVRTYLQVMYFLYNPPSFWKNTRTVHNFARKFVDKALSLTPEQVEERTQKHGYTFLYELVKHTRDPKVLQDQLLNIMVA

GRDTTAGLLSFTIFELARHPKVWNRLKDEIYHHFGSGEDSDVENISFETLKQCTYLKWVLNEILRLYPSVPVNFRVANKD

TTLPVGGGPDGTSPVFIGKGTTFAYSPYMTHRLEKFYGKDSFEFVPERWENSPKLGWAYLPFNGGPRICLGQQFALTEAS

YVIVRLAQMFPNLSSSYNGPEPCRKLVHLTMSLMDGAEIQMS

 

>CYP52A38 Candida parapsilosis

74% to CYP52A25   Candida dublinensis

CPAG_02285

MSSILDQASDFGEKLLPYLTKWYSIVFIILLTYIPLLNIRNAYVGWKLGCQDPPKFRKAGFTGVFALIEAVKKKNQGRLV

DWGDEQFDEYPNHSMYLNVTGLLKIVLTVDPENIKAVLATQFNDFSLGTRHAHFAPLLGDGIFTLDGNGWKDSRAMLRPQ

FAREQIAHVKSLEPHLQILAKHIKSTNFQTFDLQELFFKFTVDTATEFLFGESVHSLYDDKLGIAPPNDIPGREHFADAF

NKSQKYLATRTYLQMFYFLINPKEFRDCNKKVQHLAQYFVNKALNFTPEELEEKSKDGYIFLYELVKQTKNPQVLQDQLL

NIMVAGRDTTAGLLSFTMYELARNPQVWQKLRQEIVENFGDGEDARVESITFETLKKCEYLKAVLNETLRLYPSVPVNFR

TATRDTTLPRGGGADGTKPIFVPKGSTVAYTVYKTHRLEEYYGKDSKEFKPERWENIKRLGWAYLPFNGGPRICLGQQFA

LTEASYVVARLVQMFPTLESQDDTYPPKKCVHLTMNLDEGVFVKMK

 

>CYP52A39 Candida guilliermondii

66% to CYP52A14   Candida tropicalis

PGUG_01238.1

MFDVDTVHNFFTSWYGILLAVLIGYHVFDYIRIQIVMKKLGCVSPPVESDGFFGFKLLYTSLKHKKEGTLVNFIKERFET

VGKDTFSFRIAGTPVISTKNPENIKALLATQFSDFALGTRHAQFKPLLGDGIFTLDGSGWKHSRAMLRPQFAREQVAHVK

SLEPHIQMLAKHVRRAKGGAFDVQSLFFRLTVDSATEFLFGESVESLQDESIGMAKDAVDFDGKAGFAEAFNTAQVYLSI

RSLAQKAYFLVNNKEFRSSNEKVHKFADYYVQKALNSSPEELEKHSQDGYIFLYELVKQTRDPHVLRDQLLNILLAGRDT

TAGLLSFTFYELARNPQVWSKLKEEIYEKFGKGDDARLEDITFESLKKCEYLKALLNEVLRLYPSVPQNFRVAQKDTSLP

RGGGPNRDQPIFIAKGQTVTYTVYAMHRDEQFYGKDSEVFRPERWFEPETRKLGWAFLPFNGGPRICLGQQFALTEASYV

IARLAQLFPNLASHDDEYPPRKASHLTMCHQSEVKISLA

 

>CYP52A40 Candida guilliermondii

60% to CYP52A25   Candida dublinensis

PGUG_05855.1

MSELLDSIQPYLTKWYVVISALLVSFFIAHKISVARFKATHNCAASPEYYKVNWFSLPLLYRLIQVKREGRLLDFAQKIY

DDVKALTFVIKIVGVPVIITRDPENMKAVLATQFNDFALGTRHAHFKPLLGDGIFTLDGNGWKQSRSMLRPQFSREQVAH

VQALEPHLQRLAKHIRLADGETINIQDLFFKLTVDTATEFLFGQSVYSLKDAAINDPPTEDFDGRSSFANSFNTAQTYLG

TRAYLQMFYFIVNNSDFRKCCNQVHDFTRFYVQKGLDMTPEELEKKSENGYVFLYELVKQTRDPKVLQDQLLNILLAGRD

TTAGLLSFTFFELARHPRVFNKLKEEIYEAFGKGDDARVSEITFESLKKCEYLKWVMNEMLRLYPSVPVNFRVATKRTTL

PRGGGPDGNSPIYVGKGTTVAYSVYSTHRMEEYYGKDADEFKPERWAESRKLGWAYVPFNGGPRICLGQQFALTEASYIV

TRLLQMFDKLELHDDRPYPPAKSVHLTMCHQDGVYVSLS

 

>CYP52A41 Candida guilliermondii

52% to CYP52A12   Candida tropicalis

PGUG_04005.1

MLSATYVILTLVAFFTVRQVIITIKRRKFAREHGCQNFTKTVGMVSVIDNIKKVYKARREGRLTEQLQESYDLYGVDTFQ

ARAIGTSAVFTRHPENIKAMIATQFDDFDIGKRQQVFRPLLGFGIFAAEGQRWKHSRAMLRPQFAREQVAHVQSLEVHLQ

NFVKLIKEQNGSFFDIQHLFHRLTMDASTEFLFGESVGSLKSPNNEFEAAFNTSQHYLTMRATLRGAYFLVNPKEFRDAN

EVIFKTVRYYVNKALNTSPQELAKQTEEGYTFLYQLVNQTRDPQVLQDEMLSIMLAGRNTTASLLSFLFLELSRNKHIWK

KLREEVHEHFGNGENARLEDITFESMKRCNYLKWVINETLRMYPSVPTNFRHAAKDTTLPRGGGPTGDSPVFIKKNTDVY

YTIYSTHRMKEYYGKDAEEFRPERWADLKNIGWAYMPFSSGPRICLGQQFALTEASYITIRLAQIFTELDTSDHAYPPLK

IANATMRPMNGVNVRFQ

 

>CYP52A42 Candida guilliermondii

62% to CYP52A23   Candida albicans

PGUG_05670.1

MNGFEEVHNFLTSWYGIVLAAIVAYNVFDYVRVKVVMKKLNCVGPTVESDGFFGFRVIFTLLKHKREGTLVDFRKDMELD

TLRFRVAGTPMVITRNPENIKAILATQFNDFVLGGRHDHLGPLLGDGIFTLDGSGWKHSRAMLRPQFAREQVAHVKSLEP

HIQLLAKHIRKFQGEPFDIQKLFFRLTMDSATEFLFGESVDSLHDETIGFSKSAIDFDGKNEFAEAFNTSQIYLSIRALA

QQVYFLINNKEFRDANAKVHKFADYYVQKALSFSTEELDKYSQDGYIFLYELAKQTRDPKVLRDQLLNILLAGRDTTAGL

LSFTFFELARNPDIWSKLKEEIYNRFGSGDDSNLEDITFESLKKCEYLKALLNEALRMYPSVPQNFRVAQKDTTLPTGGG

PNRDLPVFIRKGQTVAYMVYALQRDQRYYGKDSEVFRPERWFEPETRKLGWAFLPFNGGPRICLGQQFALTEASYVIARL

AQLFPNLASFDDEYPPRKASHLTMCHQNGVVISMT

 

>CYP52A43 Debaryomyces hansenii

54% to CYP52A3   Candida maltosa

CAG85755.1

MNWYIIIFIAYFVRRLIILCKNYWLARQWGCEPMQEFGGRFGIISLINVIKARREGFLVDYQKQTYIETDTDTIKSRLFH

KQLIHTKNAENVKAVVATQYDDFSIGKRHKFFKPLLGNGIFASEGERWQHSRTMLRPQFARERVAHVQALEPHLQVFAKH

VRKSEGGVFNIQILFHKLTIDAATEFLFGESVDSLRDASIGYESLPTDIDGKAGFDDAFNISQNYMMLRALAQDLYFLFD

SKEFREANRKIHKFTGHYVHKALETTQEELDAKSRDGYIFLYELVKQTRDPIILQDELLSIMLAGRNTTASLLSFLFFEL

ARNPEIWSKLKREVYEHFGNGDDVKLEKITFESMKKCTYLKWVINETLRMYPSVPQNFRVAKKDTTLPRGGGKDQQSPIF

VPKGQFVLYTTYSMHRLEAYYGKDADVYRPERWENLRNIGWAYMPFSSGPRVCLGQQFALTEASYITIRLAQMFPNLESH

DSKYPPPKLTNSTMRHLDGVFISLH

 

>CYP52A44 Debaryomyces hansenii

63% to CYP52A25   Candida dublinensis

CAG85832.1

MSTDKLKSYVEEISPYLTQWYVIALGGILVWYIGTSIKAYQFKQKYGCKEPPLVPGGFLSIKLLLDLLKTKSEGTMLEYA

EHFFSLVKSQTFKLKLATVPIIFTQDPENIKAVLATQFNDFALGTRHAHFKPLLGDGIFTLDGDGWKNSRLLLRPQFARE

QVAHVQTLEPHLQTLAEHISNKKGETFDIQELFFRLTVDAATEFLFGESVYSLRDGTVKDVAPLDFDGKSSFADSFNLSQ

SYLASRAYSQMFYFLINNKEFRNSNTKVHKFCKHYVEQALATSQDELDKKSKDGYIFLYELVKQTRNPKILQDQLLNILL

AGRDTTAGLLSFTFFELARNPDVFEKLKNEIYTHFGDGANPRIETITFESLKRCEYLKFVLNEVLRLYPSVPINFRVSTK

DTTIPRGGGPDKQDPVYVAKGTTVAYSVYTTHRLEQYYGKDSREFRPERWAENKKVGWAYVPFNGGPRICLGQQFALTEA

SYVVVRLIQMFPNLVSRDDRPYPPRKNVQLTMCHQDGVFISLNK

 

>CYP52A45 Debaryomyces hansenii

62% to CYP52A14   Candida tropicalis

CAG88380.1

MSSDTFHEFGVLFDRIQPYLTSLWAICIGAYILFSISTRINDRILMKKLGAKPMQMNAPNSFWGIPLMFRLLKAKREGTM

VDVTKERYKELGVDTFNVNIAGTHAVVTRDPENIKAILATQFNDFALGKRHTHFKPLLGDGIFTLDGAGWKHSRTMLRPQ

FAREKVAHVKALEPHLQTLAKHIINSKGERFDIQPLFFRLTIDSATEFLFGESVENLHDESVGLTRDPVDFDGKSGFADA

FNTSQTWLASRAVTQNLYFLVDGRSFRSSNAKVHKFADYYVRKALEASSEDLEKASRDGYIFLYELVKQTRDPVILRDQL

LNILLAGRDTTAGLLSFTFFELARNQDVYDKLKEEIYEKFGEGEDSRVDEITFESLKKCEFLKCVLNEILRMYPSVPQNF

RFAIRNTTLPKGGGPDGKSPIFVSKGKNLFYSMYSAHRNPKYYGKDAEEFRPSRWLEPEIRKVGWAFLPFNGGPRICLGQ

QFALTGASYIVVRLIQMFPNLSSFCEEYPPRKNTQLTMSHQNGVYIGMF

 

>CYP52A46 Debaryomyces hansenii

63% to CYP52A14   Candida tropicalis

CAG88381.1

MSSSAHELFRSIEPYLTNWWIICIGTYVLFSILTWINERILMKKLGAKPIQFIAPDPFWGIPLMFRLLRKKKDGTMVDMT

KERFEEFGIETFSFRVGGTPVITTKDPENIKAMLATQFNDFALGKRHTYFKPLLGDGIFTLDGAGWKHSRAMLRPQFSRE

QVAHVRALEPHLQTLAKHIINSKGERFDIQPLFFRLTIDSATEFLFGESVENLHDESVGLTRDPVDFDGKSGFADAFNTS

QAWLASRAVSKNLSLFVDGKEFRNSNAKVHKFADYYVRKALEASPEDLEKASRDGYIFLYELVKQTRDPVILRDQLLNIL

LAGRDTTAGLLSFTFFELARNQDVYDKLKEEIYEKFGEGEDSRVNEITFESLKKCEFLKCVLNEILRLYPSVPLNTRCAT

KNTTLPRGGGPDGQSSIFIPKGKSVLYSVYATHRNPKYYGKGAEEFRPSRWLEPEIRKVGWAFLPFNGGPRICLGQQFAL

TEASYIIVRLIQMFPNLNSFCEKYPPRKSSHLTMCHQDGVSIGMF

 

>CYP52A47 Debaryomyces hansenii

65% to CYP52A14   Candida tropicalis

CAG88382.1

MSSNFHEVVESIEPYVTNWWFICIGAYVLFSILTWINKRILMKRLSAKPMQVTARDAFVGIPLVFNLLKAKREGTMVDMT

KERYEEFGVDTFDFRIAGTHAIATRDPENIKAVLATQFNDFVLGKRHAHFKPLLGDGIFTLDGAGWKHSRAMLRPQFARE

QVAHVKALEPHLQTLAKHIINSKGERFDIQPLFFRLTIDSATEFLFGESVESLHDESVGLTRDPVDFDGKSGFADAFNNS

QAWLASRAVSQNFYFLLNGKDFKDSNANVHKFADYYVNKALETSPEDLEKASRDGYIFLYELVKQTRDPVILRDQLLNIL

LAGRDTTAGLLSFTFFELARNQDVYDKLKEEIYKKFGEGEDSRVDEITFESLKKCEFLKCVLNEILRMYPSVPQNFRFSI

KNTTLPRGGGPDGKSPILIPKGQNLLYSVYATHRNPKFYGKDADEFRPSRWLEPEIRKVGWAFLPFNGGPRICLGQQFAL

TEASYIIVRLIQMFPNLGSFCEEYPPRKSSHLTMCHQDGVFIGMS

 

>CYP52A48 Lodderomyces elongisporus

73% to CYP52A38   Candida parapsilosis

LELG_04957

MSSTFVQQVASLGQEIVPYFTKWYTVLILLVVTYIAAFNIKDAYMTWKLGCKNPLYYKPAGYTGVYSLYEIIQRKNKGEL

AEFAQEAFDEYTTNTVYVKIGGLMKIMFTVDPENIKAVLATQFNDFSLGIRHAHFAPLLGDGIFTLDGDGWKDSRAMLRP

QFAREQIAHVKALEPHVLMLAKQIANTKYETFDLQELFFRFTVDTSTEFLFGESVHTLYDDKIGVAPPNDIPGREAFADA

FNFSQHYLATRTYSQVFHWLFNSKEFRENNAKVHHLAQYFVKKALNFTPEELEEKSQDGYTFLYELVKQTRNPKTLQDQL

LNIMVAGRDTTAGLLSFCMFELARNPEIWQRLRTEVLDNFGDGSTKEQIDSITFENLKKCEFLKSVLNETLRLYPSVPIN

FRTATRDTTLPHGGGSDGSKPIFVPKGSTVAYAIYKTHRLEEYYGKDSQEFKPDRWTDMKRLGWAYLPFNGGPRICLGQQ

FALTEASYVLARLAQLFPTLQSRDETYPPKKCIHLTMNHAEGVFVGMK

 

>CYP52A49 Lodderomyces elongisporus

72% to CYP52A37   Candida parapsilosis

LELG_03506

MAISLETLPLAIVQHLFTWYGAITLLITTYFTGLYIRREIFRRQRGCMEPFVYPEGGPLGIKLGYDLQDKKKKGEIVDFM

CKLYHKFDRVKTFSAYLFGVRVVVGVEPENLKAVLATQFNDFGLGTRHAHFKPLLGNGVFTLDGEGWKESRQMLRPQFAR

DQIAHVQALEPHIQILAKHIRASKGKSFDIQELFFRFTVDTSTEFLFGESVHSLYDASIGLECDPEVAGFAEAFNETQKD

LAVRTYLQVLYFLYNPPSFWKNTKVVHKFAKRFVNKALNLSKDELEEHCKHHYTFLYELAKHTRDPKVLQDQLLNIMVAG

RDTTAGLLSFTFFELARNPEVWQKLKDEIYQHFGSGEDADVSKITFETLKQCTYLKWVLNEILRLYPSVPINFRTALKDT

TLPTGGGPDGTAPVFIGKGTTFAYSVYLLHRIEEYYGKDANEFRPERWADLKQLGWAYLPFNGGPRICLGQQFALTEASY

TICRLAQMFPNLASNYDQPDPCKKLIHLTMSLMDGAWIEMS

 

>CYP52A50 Lodderomyces elongisporus

71% to CYP52A37   Candida parapsilosis

LELG_03505

MMETIISNTGHALQPPYSSHTIWFGILFIIFGTYFICIFIKREIFRRAHHCKEPIIFPEGGPLGLRLGYLLQRLKTTGEL

VDFPMVLYEKFGRRTTFSAYLFGVRVVASVNPDNLKAVLATQFNDFGLGTRHAHFKPLLGNGVFTLDGEGWKESRQMLRP

QFAREQVAHVQTIEPHLQTLAKHIKTHNGGTFDIQELFYKLTVDTSTEFLFGESVHSLYDASIGLECDPEVAGFAEAFNE

TQKALAVRTYLQVLYFLYNPPSFKRNTKIVHKFAKRFVNRALCLTPKELEEHCKHNYTFLYQLAKHTRDPQVLQDQLLNI

MVAGRDTTAGLLSFTIFELARHQNVWEKLKKEIYQHFGSGEDALVNKITFETLKQCTYLKWVLNEILRLYPSVPINFRVA

TKDTTLPTGGGLDEKSPVFIGKGTTFAYSPYLLHRMPMHYGEDATVFRPERWADLKQLGWAYLPFNGGPRICLGQQFALT

EASYTICRLAQMFPDLKSMDDSPDPCKKLIHLTMSHMDGVWIQMSE

 

>CYP52A51 Lodderomyces elongisporus

75% to CYP52A35   Candida parapsilosis

LELG_03309

MLLDEFITYLNHWYVIIPLAFTTYHVFHYIYLNQLAKKFGAKPITNVLSDGWFGFKNGTQAIALKNKGQAVEWAQEKYYE

TKHPEIPTFKERIFGLYLVFTKDPENIKAMLATQFSDFSLGHRLAYFDPLLGKGIFTLDHEGWKDSRAMLRPQFAREQIA

HVKSLEPHIQYLFKHIDKNQNIQSKNKIENADYETSISASYFDIQELFFRFTVDSATEFLFGGSVSSLQDETIGCDTRDI

DFAGRRQFADCFNKAQNYLATRNLLQKLYWLVNPKEFRECNKVVHEFTNYYVNKVLSYSPEELEKISDGYVFLYELAKQT

RDPNVLRDQSLNILLAGRDTTAGLLSFVIFEMAKNPQMWTKLRNEILERFGTTNLEDITFENLKKCEYLKAVLNETLRLY

PSVPRNARVATRNTTLPRGGGPDGLSPIFIAKGSTVGYNISACQRDETHYGKDVDEFRPERWFEESTRKLGWAYLPFNGG

PRICLGQQFALTEASYVITRIAQTYSKLELKPGYGYPPKRMTHLTMCLFDGCWVKMERAQV

 

>CYP52A52 Lodderomyces elongisporus

70% to CYP52A5   Candida maltosa

LELG_00044

MSDLLPPIIQEYATKWYILLPAIVLVYLTFSFLFEIYLKRKLGAKGFTNTERDGYFGFYTPFLLLRLKKEGNLVDFGTER

YQFLQNPDVPTFKLRMFGIPIVTTKDPENIKAILATQFSDFLLGLRHAQFMPLLGDGIFTLDGQGWKDSRQMLRPQFARE

QISHVKLLEPHMQVFFKHVRKHKGMTFDIQELFFKLTVDSATEFLFGNSVECLKDSSIGMRPNSADFEGKEQFADAFNYS

QTYLSTRVVFQKFYWLLNGKKFKECNKIVHSFAQYYVNKALQLTPDDLQKQENYVFLYELAKNTRDPQVLQDQLLNIMVA

GRDTTAGLLSFVFFELARNQDVLAKLKEEIYGKFGFGADARVDEITFESLKGCEYLKAVLNECLRLYPSVPNNFRTALRD

TTLPRGGGKDGMSPILIRKGQNVIYSVYALHREEKFYGKDAAEFRPERWFEPETRKLGWAFVPFNGGPRICLGQQFALTE

ASYVTTRLIQEFEHLTMDPDTEYPFKKMSHLTMSVYGGVNVQMY

 

>CYP52A53 Pichia stipitis

65% to CYP52A40   Candida guilliermondii

JGI model e_gww1.3.1.449.1

MSAELAFEYLTKWYSILIGAALIYGIARYIKIQLFIRKHGCEETPFLPDAKWFAIPIMSRVLKAKNEGRLVDLAQSFMTS

DRRTTHVYLGPARIIFTIDPENMKTMLATKFNDYALGFRHTHLAPLLGDGIFTLDGEGWKHSRSMLRPQFAREQVAHVRA

LEPHVQVLMKHIRLNKGKTFDLQELFFKLTLDTSTEFLFGESIYSLYDSSIGLTPPTDIQGRSEFADAFNTSQKYLGTRA

WLQFMYWVVQNREFYQCNAKVHKVAKYYVKRALNFTPDELEKASANGYTFLYELVKQTRDPVVLQDQLLNILVAGRDTTA

GLLSFTFFELARNPDVFEKLKNEIYEHFGKGDESRVEDITFESLKQCEYLKFVLNEALRMYPSVPLNFRVSTKDTVLPNG

GGKDGTKPVFVGKGTTVAYTVYCTHRDEKYYGKDANVFRPERWATLNKLGWAYLPFNGGPRICLGQQFALTEASYVIVRL

LQNFPNLVSKDDRPYPPAKSMHLTMCHQDGIFVELS

 

>CYP52A54 Pichia stipitis

63% to CYP52A44   Debaryomyces hansenii

JGI model e_gww1.3.1.459.1

MYEKTIIALLYLSKWYVLLGSAVAVLALYRYITVQLIIRKHGCKDPIKFTQGGFLCIPIILELLRRMKTGEMIDEGFRLF

NEYPDTTQYVNLFGVRVLLTGEPEVYKAVLATQFNNFALGFRHSHFAPLLGDGIFTLDGEGWKNSRAMLRPQFARQQVSH

VQMLEPHIQTLAKHIKARKGTTFDLQELFFRLTMDSATEFLFGESVRSLHDEVIGMQRPDIEGISNFSEAFNTSQRYLAV

RAYSQVLYWLTNPKEFRDCNAKVQKVAQYFVNKALSFSDSELEEKSKKGYIFLYELTKQTRDPKVLQDQLLNILIAGRDT

TAGLLSFTFYELARNPAIFEKLKAAIYADFGRGDVSEISFESLKKCEYLKFVLNEALRLYPSVPVNFRVATKDTVLPTGG

GPDGQSPMFVRKYTTVAYGVYFTHRMKQFYGEDAEVFKPERWAENRKLGWAYLPFNGGPRICLGQQFALTEASYVVVRLI

QMFPNIVSKDDGPYPPRKSVQLTMCLQDGVKIQMS

 

>CYP52A55 Pichia stipitis

63% to CYP52A14   Candida tropicalis

JGI model  fgenesh1_pm.C_chr_3.1000237

MANFIEFVTTNWYIIIPALLVLHKVFDLLYVQYLYRKLGAKPCTNQTDDHAFGIRAGFEMLKKKNEGTVVDFGAERFESR

IDPKIPTFSMRLFLIPIVLTRDPENIKALLATQFNEFVLGSRFEQLAPLLGKGIFTLDGEGWKHSRAMLRPQFAKEQVAH

VQSLEPHIQALAKHVRNAKGKLFDIQELFHRLTVDSATEFLFGQSVESLRDESVGMADEATDFAGKSTFAASFTIAQNWL

ANRAVAQKFYFLINPKEMRDSIKDVHRFVDYYVQVALDTPQDELDKKSKDGYIFLYELVKQTRDPYVLRSQLLNVLLAGR

DTTAGLLSFAFFELARRPDIWSKLKDEIYENFGLGENSKVDEITFESLKRCEYLKAFLNETLRLYPSVPVNFRVATKDTT

LPRGGGKDGSEPILVRKGQSVFYSVYATHRSEAYYGKDRHVFRPERWFEPSARKLGWAYLPFNGGPRICLGQQFALTEAS

YVVARLIQLFPNIENYEPEEVYPPFKNSQLTMNLLNGLHIGLY

 

>CYP52A56 Pichia stipitis

59% to CYP52A38   Candida parapsilosis

JGI model  fgenesh1_pm.C_chr_8.1000042

MFMTTDPENFKAMLATQFNDFSIGRRYQILSPVIGDSIFTLDGEGWKHSRAMLRPQFVREQVGHVQALEPHLQLLAKHIR

SYKGETVDLQQLFTKFTLDTATEFLFGQSVHTLYDERIGMKTPDDVPYAKDFTDGLFITQKYTSERGYAQQFYWLIDGKE

FRTAIANVHKFARFYVDRALNFSQAELEKKSQESYTFLYELVQQTRDPKVLQDQLLAIMLAGRDTTSSLLSFIFYELSRN

PGIWEKLKKEVYENFGSGTEKDIAKITFESLKKCNYVKWVINETLRMYPTVPVNLRVSNKDTLLPKGGGEDGKSPIFIPR

GTTVGFRVYSTQRNKEYYGEDPDVFRPERWADIGKLGWAYLPFLGGPRTCIGQQFALTEAGYILVRIAQLFPNLKSKNSV

HYPPKKTLNVIFNLFEGCLVEMGE

 

>CYP52A57 Pichia stipitis

62% to CYP52A39   Candida guilliermondii

JGI model  e_gww1.3.1.1392.1

MTVETIVQYLLCWQVYVLAVLVYHIFNWIHEQILYKKFGASPCINNERGGFLGIRLLRTLLKAKLDGTLSDVVKNRYYEM

EHPEIETFTLRVFSQTVIATRDPANLKAILATQFNDFSLGIRHAQLYPLLGDGIFTLDGEGWKHSRAMLRPQFAKEQIAH

VQSLEPHIQVLAKHIRKSEGKSFEMQELFFRLTLDCATEFLFGESVESLRDASIGMARADVEVEGKDRFAEAFNLAQTQI

ATRSMMNKMYFLYNTEELRNSCDVVHRFTDYYVNLALRTSTAELDKKSKSGYTFLYELVKQTRNPQILRDQLLNILLAGR

DTTAGLLSFAFFELARQPHIWAKLKDEIYSAFGSGENSRIDEITFESLKRCEYLKAVLNETLRMYPSVPNNGRIAVRNTT

LPRGGGPTGTEPMLVRKGQKVVYSVYTTHRSKTHYGEDAEVFRPERWFEPSSRKLGWAYLPFNGGPRICLGQQFALTEAS

YVVTRLIQMFPNISSDPTIEYPPRKASQLTMCLQDGLLISLY

 

>CYP52B1 C.tropicalis alk6 Z13013

MSLTETTATFIYNYWYIIFHLYFYTTSKIIKYHHTTYLMIKFKA

SPPLNYINKGFFGIQATFTELKHLICHTSIDYAIDQFNNVPFPHVHTFVTKVLGNELI

MTKDPENIKVLLRFPVFDKFDYGTRSSAVQPSLGMGIFTLEGENWKATRSVLRNMFDR

KSIDKVHDFEPHFKTLQKRIDGKVGYFDIQQEFLKLGLELSIEFIFGQVVSEDVPHYD

DFTQAWDRCQDYMMLRLLLGDFYWMANDWRYKQSNQIVQAFCDYLVQKSLENTCNDKF

VFVHQLAKHTTNKTFIRDQALSLIMASRDTTAELMAFTILELSRKSHHLGKLREEIDA

NFGLESPDLLTFDSLRKFKYVQAILNETLRMYPGVPRNMKTAKCTTTLPKGGGPDGQD

PILVKKGQSVGFISIATHLDPVLNFGSDAHVFRPDRWFDSSMKNLGCKYLPFNAGPRT

CLGQQYTLIEASYLLVRLAQTYETVESHPDSVYPPRKKALINMCAADGVDVKFHRL

 

>CYP52C1 C.tropicalis alk7 Z13014

 

MYQLFCFLAGIIVVYKAAQYYKRRTLVTKFHCKPARISPNKSWL

EYLGIASVVHADEMIRKGGLYSEIDGRFKSLDVSTFKSITLGKTTYVTKDIENIRHIL

SATEMNSWNLGARPIALRPFIGDGIFASEGQSWKHSRIMLRPVFAKEHVKQITSMEPY

VQLLIKIIKNHEGEPLEFQTLAHLFTIDYSTDFLLGESCDSLKDFLGEESNSTLDTSL

RLAFASQFNKTQQQMTIRFMLGKLAFLMYPKSFQYSIQMQKDFVDVYIDRVVGMSEEE

LNNHPKSYVLLYQLARQTKNRDILQDELMSILLAGRDTTASLLTFLFFELSHHPEVFN

KLKEEIERHFPDVESVTFGTIQRCDYLQWCINETMRLHPSVPFNFRTAANDTVIPRGG

GKSCTDPILVHKGEQVLFSFYSVNREEKYFGTNTDKFAPERWSESLRRTEFIPFSAGP

RACLGQQLPRVEASYVTIRLLQTFHGLHNASKQYPPNRVVAATMRLTDGCNVCFI

 

>CYP52C2 Candida maltosa D12718

MIDALYILIVALVIYKTAQFVHRKSLEKKHHCQPVKQIPLVSIL

SGLGFDMFFKDTAEMTKNGGLHKKLQQMLESLQTTTFRSRMLTGSQIVTMEPENERTM

CSSAHMKDWTIGYRPFALKPLLGDGIFSSEGESWKHSRIMLRPIFAKEHIKQITAMEP

YMLLLIEIIKSSSANEGPVDLQPLFHAFTIDYASDFLFGESCDVLKENLGGKSTSGMD

AQVKRDFASVFNDVQNYLTKRMMLGPLAFLVSSKDFHDGIKKQHEFVSYFVQKAISMS

DEELNDESKNYVFLYQLAKQTKDAKVLQDELLSILLAGRNTTASLLSFLFFELSHHEN

VWTTLKEVVDQSFPDVESITFETIQNCDYLRWCLFESLRVNPSVPFNSRTANKDTILP

RGGGEDCSHPILVKKGDQVLFPLYASNRQEKYFGRKPEEFIPERWRDLPKTGGPAFMP

FSTGPRMCLGQQFALIEASYVTIRLVQTFSKLKSHSLEYAPKRLVAATIRLIDGCFVS

FE

 

>CYP52C3 Candida albicans SC5314

60% to CYP52C2 orf19_6574

MLILLPIAVIAASIYYIAEYWKRESIKRKYDCQPVKQAPLVTWYDIFGIKALVDNSNLTKSGYFYQDIQRKLDNLDTTTM

KTTALGKNQIITIEPENFRTMCSSADLNNWTIGTRPIALKPLLGNGIFSSEGESWKHSRIMLRPIFAKEHIKQITAMESY

IQSLIKVIKLQQQGEGEFDLQHLFHCFTIDYATDFLFGESCDCLKSILGETSISTMSEEEKSKFAPNFDVMQYHMTKRLV

FGYFSFLVNPPEFKNAVKHLHEFVFHFVDRAINFTDEELNDPSKNYVFLYQLAKQTKDRKVILDELLSVLLAGRNTTASL

LSFMFFELAQNKPVWNKLKKEITEHFPDVESITFETIHNCEYLRWCIHESLRINPPVPINTRTANRDTVLPKGGGKGNNY

PIFVRKGEQVIFPLFSSNRQERFFGPEPEKYIPERWANLPRNGGVAFGPFGTGPRLCLGQQLALVEASYVTIRLLQTFSQ

LDSNHTECRRSVGATMRLMDGCQVTLK

 

>CYP52C4 Candida dublinensis

94% to CYP52C3   Candida albicans (ortholog)

cdub_588-g100.1

MLILLPVAVLAAIIYYIAEYWRKESIKRKYDCQPVKQAPLVTWYDIFGIKALIDNSNLTKSGYFYQDIQRKLDSLDTTTL

KTTALGKNQIITIEPENFRTMCSSADLNNWTIGTRPIALKPLLGNGIFSSEGESWKHSRIMLRPIFAKEHIKQITAMESY

IQLLIKVIKIQQQQQEEFDLQHLFHCFTIDYATDFLFGESCDCLKLILGETSISTMSEKEKSKFAPNFDIMQYHMTKRLV

FGYFSFLINPPEFQNAVKHLHEFVFHFVDRAINFTDEELNDPSKNYVFLYQLAKQTKDRKVILDELLSVLLAGRNTTASL

LSFMFFELAQNKPVWNKLKQEINQHFPDVESITFDTINNCEYLRWCIHESLRINPPVPINTRTANKDTVLPKGGGKGNNY

PIFVRKGEQVIFPLFSSNRQERFFGPEPEKYIPERWANLPRNGGIAFGPFGTGPRLCLGQQLALVEASYVTIRLLQTFSE

LDSNHTECRRLVGATMRLMDGCQVTLK

 

>CYP52C5 Candida parapsilosis

48% to CYP52C3  Candida albican

There is one 52C seq per Candida species except this species has two.  They all seem to be orthologs even though the percent identity is not high

CPAG_01250

MNYILYSALAAIYIIYRVIDHVQVTRKAQKYGCKPVAQVPKRTLLSWLGLQLILNLKRNHERGRAHEALDEAFDRLKTTT

FATYVGIEPNVFTVEPENFRHMVSSSHTSEFNLGVRLDVFSILLGDGIFSSEGTSWKHSRIMLRPFFARENIKQIQSMEP

FTQSIIKAIKQAPGMTIDMQEIFQDFTMDYTTHFLLGESCGCLKTYLGEPTSDSISTEVKSSFAETFDKGAEYMLQKLVM

GRYHFLYRPKQLYVDVKNQHAFVDYYVQKALRMNQQDLKDSSNDGTLFLYDLAQQTKDPKVLRDEILSIIVAGRSTTSAL

MTFLFLELGRHQDVFERLKGIVRAHFPDIESITFESIQQCEYLRWCLNETLRHDPPVPFNSRTVTQDTILPKGGGQDNES

PIFVRKGTKIVYPLWTAHRMEKYFGKDPYTFNPDRWSSLPANGGFAFMPFGAGPRNCLGQQLALLETSYVTIRLLQTFNS

LKADSGEVRKKSTATMVLMDGCKLTFG

 

>CYP52C6 Candida parapsilosis

50% to CYP52C2   Candida maltosa

CPAG_01665, 53% to to CYP52C5 this species

MTYIPYLIVIASYIIYKLLHRVQLHQRAIKLGCKPVKHIPPRNLLDRLGIYEIVHRKKLMHSGTTFEMLEEAFDRAQVKT

FEGTALFSSFIMTIEPENLRHMMSSAHFNEWNIGYRPQAFGILLGDGIFSSEGTQWKHSRVMLRPSFTKENIKQITKMEP

FAARVIDLIKQKSGQAIDMQDVFQFFTMDFTTDLLLGESSDSLKDALGQHSSTGIDPELKRQFSIAFDESSNYLMTRMIL

GPLMFLVNPKTLQDAIKIQHKFVDYYIEKALAMTSHELNEKSDDGTIFLYQLAHETKDPINLRNQILSIILAGRNTTSGL

MTFLFAQLSKNPEIYCKLRDIIRQDFPNVESITFDSIQNCDYLRWCINETLRINPSVPFESKTASVDTTLPRGGGVDEKA

PIFIAKGTRIVYPLWVANRNEEHFGENTGEFIPERWEQLPRSGGIAFMPFSVGPRACLGQSLALTEAAYITIRLLQTFDS

CESHLGPEPLRKTSSATMRLMDGCELSFK

 

>CYP52C7 Lodderomyces elongisporus

54% to CYP52C6   Candida parapsilosis

48% to CYP52C3   Candida albicans

LELG_05768

MMIHLPLFIVLTLLLVLYNIIRFYRKQVIKRKYKCQKIPNAQTSTFLSYLGLEFIVNYNKIQEEGRIHQNMYKQFKDLKT

STLAQTMLFLKVVFTTDPENYRYMSSSANFNNWTIGDRPDAFDPLLGDGIFSSSGESWKHSRIMLRPFFSKDHIKQIEYM

EPYVKDVVRLINIYQRDGVVVDMQDIFQYFTIDYATKFLLGESCDTLKDALDEPTSVTIDPELKSRFAKAFDISLKYLML

RVSLGKPLMWLVNPKELREANKIQHDFVRYYINKALAMNEEELNKHSDNGNCFLYEIAKQTKDPKILQDEVMSIILAGRN

TTSSLLSFLFFELSRDANKEILKKLVDAVRSNFDSIESITYEKIHNCDYLRWCLYETLRFHPSVPLASKTAAVDTILPKG

GGLDNESPVFVPKGRRVVYPLYTANRNSKFFGSDTEVYRPERFADLPKSGGPAFMPFSTGPRLCLGQQLALTEASYITIR

LLQTFDIIEKVGDEPYPPRTSSSATMRLMDGAKVRMRTSSSTTP

 

>CYP52D1 Candida maltosa D12716

MAIFTPELWLICFAVTVYIFDYIYTKYLMYKLGAKPITHVIDDG

FFGFRLPFLITLANNQGRLIEFSVKRFLSSPHQTFMNRAFGIPIILTRDPVNIKAMLA

VQFDEFSLGLRYNQFEPLLGNGIFTSDGEPWKHSRIMLRPQFIKSQVSHVNRLEPHFN

LLQKNITAQTDNYFDIQTLFFRFTLDTATEFLFGQSVHSLNDGENSLQFLEAFTKSQA

ILATRANLHELYFLADGIKFRQYNKMVQDFSQRCVDKVLNMSNSEIDKLDRYFFLYEM

VKITRNPQVLRDQCLNILLAGRDTTASLLSFAFFELALNEPIWIKLRTEVLHVFQTSL

ELITFDLLKTKCPYLQAILHETLRLYPSVPRNARFSKKNTTLPHGGGVDGMSPILIKK

GQPVAYFICATHVDEKFYTKDALIFRPERWCEEPLIKKNLAWSYLPFNGGPRICLGQQ

FALTEASYVLTRLAQCYTKISLQPNSFEYPPKKQVHLTMSLLDGVHVKISNLSIS

 

>CYP52D2 Candida tropicalis

AY230503

MAISSLLSWDVICVVFICVCVYFGYEYCYTKYLMHKHGAREIEN

VINDGFFGFRLPLLLMRASNEGRLIEFSVKRFESAPHPQNKTLVNRALSVPVILTKDP

VNIKAMLSTQFDDFSLGLRLHQFAPLLGKGIFTLDGPEWKQSRSMLRPQFAKDRVSHI

SDLEPHFVLLRKHIDGHNGDYFDIQELYFRFSMDVATGFLFGESVGSLKDEDARFSEA

FNESQKYLATRATLHELYFLCDGFRFRQYNKVVRKFCSQCVHKALDVAPEDTSEYVFL

RELVKHTRDPVVLQDQALNVLLAGRDTTASLLSFATFELARNDHMWRKLREEVISTMG

PSSDEITVAGLKSCRYLKAILNETLRLYPSVPRNARFATRNTTLPRGGGPDGSFPILI

RKGQPVGYFICATHLNEKVYGNDSHVFRPERWAALEGKSLGWSYLPFNGGPRSCLGQQ

FAILEASYVLARLTQCYTTIQLRTTEYPPKKLVHLTMSLLNGVYIRTRT

 

>CYP52E1 Candida apicola X76225

MIIGLSDAFALGGIALSFLVAYQFIYFYFIYSPRAKKLGCAPPV

IVFSFPLGLPALYKFATAMLHDNLLEYISIRIADMKVRTGFQTLAGQRWLVTLEPENI

KTVLATSFKDYSLGFRYDIMYGLLGNGIFTLSGDGWKHSRALLRPQFSREQVSHLESM

RTHINLMINNHFKGGQVVDAQALYHNLTIDTATEFLFGESTNTLDPDLAQQGLPGPKG

LVTGEQFAEAFTSALEILSVRVIVGAAWFLIWTPKFWRSCKVCHNFIDYFVYKALATP

MEKDQEADRYVFIRELTKETSDPRVIRDQALNILLAGRDTTAGLLSFITYYLGAYPEV

YAELREAVLSEFGSTDVETPTFEQLKQCKVLQNVIREVLRLHPNVPLNFRQAIVDTKL

PTGGGPNGDQPVFVPKGQNVFYSTYSMQRRTDIWGPDATTFRPDRWNEPREALASGWD

YIPFNGGPRICLGQQFALTEASYTIVRICQEFSRIEVLHPDVITSKNSMKQRMRLTQT

ASGGVITRFIR

 

>CYP52E2 Candida apicola X87640

MNINFSDALMLGGISLSFLLASQAIYFYFIYSPRAKKLGCAPPP

IFFSFPLGIPDLIRLVNAWFHDDLLEWFTHRFEEFGRRTAFQSVAGQLWIGTIEPENI

KTMLATSFKDYSLGFRYNAMYGLLGNGIFTLSGDGWKNSRALLRPQFSREQVSHLESM

RTHINMMINNHFKGGQVVDAQVLYHNLTIDTATEFLFGESTNTLDPALAQQGLPGPKG

LVTGEQFAEAFTSALELLSVRVMAGAAWFLVWTPKFWRSCKVCHNFIDYFVYKALATP

MEKGQDADRYVFIRELTKETSDPRVIRDQALNILLAGRDTTAALLSFTTYYLGAYPEV

YDELREAVIADFGSADAEPPTFEQLKQCKVLQNVIREVLRLHPNVPLNFREAIADTTF

PTGGGPNGDQPIFVPKGQKVFYATYVMQRNAGIWGPDSTSFRPDRWNEPREALASGWD

YIPFNGGPRICLGQQFALTEASYTLVRICQEFSRIEVLHPDVITARNVMKQRMRLPNS

SSGGVITRFIR

 

>CYP52E3 gene2 Candida bombicola 92% to 52E2, 82% to 52E1

EU552420 alternative name Starmerella bombicola

MNINFSDVLVLGGISVSFLLAYQAIYFYFIYSPRAKKLGCALPPVFFSFPLGIPEVXRLVNAWFNDDLLEYFTFKFEEFQRKTGFQSVAGQLWIGTIEPENIKTMLATSFKDYSLGFRYEAMYGLLGNGIFTLSGEGWKHSRALLRPQFSREQVSHLESMRTHINMLINNHFKGGKVVDAQVLFHNLTIDTATEFLFGESTNTLDPALAQHGFPGPKGLVTGEQFAEAFTSALELLSVRVMAGAAWFLVWTPKFWRSCKVCHNFIDYFVFKALATPMEKDQEADRYVFIRELTKETSDPRVIRDQALNILLAGRDTTAALLSFTTYYLGAYPEVYDELREAVIADFGKEDAEPPTFEQLKQCKVLQNVIREVLRLHPNVPLNFREAITDTKFPTGGGPNGDQPVFVPKGQKVFYATYVMQRNEGLWGPDSTTFRPDRWNESREAIASGWDYIPFNGGPRICLGQQFALTEASYTLVRICQEFSRIEVLHPDVITSRNVMKQRMRLTNSSSGGVIAKFIR

 

>CYP52F1 Yarrowia lipolytica gene for ALK1 AB010388

CAG80007.1

MSNALNLSLALGVFLLAYYGFSVIQYRIKTRKLEKKWKCGKPKD

ISRFPFSASFFIPFLVESKKNRLLEFVQWMFESQVYPGYTCKTTVFGVDMYHTVDPEN

LKAVLATQFKDFCLGERHAQFLPVLGNGIFTLDGQGWQHSRAMLRPQFARDQVSDVEM

IEEHIQYMTSRIPKDGSAFDAQELFFNLTLDTATEFLFGQSVGSQTVETNPTAVPTDM

PVHLRKSFQEDFNTAQEHLGQRARLQMFYWAWRPRELYSSGERVHAFVDHYVKKALEE

SEKHVDDGKYVFLRELAKETKDPIVLRDQALNILLAGRDTTASLLSWCLYLMARRPEV

YAKLREEVIENLGDGEDLSTITFESLKRCDYLRYVLNEVLRLYPSVPANMRYATRDTT

LPRGGGPDGMQPIVVRKGNLVSYHVFTTHRLKEFWGEDAEEFRPERWYEDGASQAKGW

EYLPFNGGPRICLGQQYALTEAGYALARIAQLYDTIENADDKPEPPVKFHALTMCHHT

GVLVKLYNSKTTKAQ

 

>CYP52F2 Yarrowia lipolytica gene for ALK2 AB010389

CAG77659.1

MIILYVLAVAVSFLIFKRVTYTMRSRELAKKWHCEEPHNLNEFP

LNLPLFFLIINASRRHELLDTLLGLFRSFAPTKTVKQVLLGSFTIIPTNDPENIKAVL

ATQFKDFCLGQRHGQLAPVLGDGIFTLDGQGWQHSRAMLRPQFARDQVSDVEMIERHV

QMMLLRIPNNKKFDIQELFFNLTLDTATEFLFGQTVGSQTVEMPNEDKSTVSDMPKDM

RKSFQEDFNVAQHHGGIRTRFQMFYWLWRPTELFSSSKRVHAFVDHYVEKALANSDEE

KSDDKYIFLRELAREVKDPRVLRDQALNILLAGRDTTAGVLSWIVYELARHPEVWKKL

RAEIHQDFGDGSDLSQITFEGLKRCEYLRFVINETLRLYPSVPLNVRYASRDTTLPRG

GGPDESKPILVRKGDTIVYNVFSMHRTEEFWGKDCDEFRPERWAEKGSRGWEYLPFNG

GPRICLGQQYALTETSYVITRICQLFTNIENADTAVEPPQKLHALTLCHLNGVFVKMT

RDEAAFAETEKLINA

 

>CYP52F3 Yarrowia lipolytica gene for ALK3 AB010390

CAG79910.1

MIIIETLIGAVVFVAVYVAFVKLDYYRRKAKFETSDMPVAYNGL

LGWKGLRHMLTVFNNDIGPVGWREVFATYGKTLKYYAFPSNTILTYDPDNIKAMLATQ

FKDFSLGLRKEALAPSLGYGIFTLDGSSWSHSRALLRPQFSREQISRLESVETHVQEM

MSCIDRNQGAYFDIQRLFFSLAMDTATDFLLGEAVGNLQEILHPEMPRTGTTFQVAFD

RAQRLGSLRIICQEAFWVVGSLFWRRDFNNTNQHIHDYVDRYVDKALLARKEKSEIYT

NPDKYIFLYELARETTNKITLRDQVLNILIAGRDTTASTLSWIFMELAKKPDIFHKLR

EAILNDFGTSCESISFESLKKCDYLRQVLNEGLRLHPVVPVNLRVAVRDTTLPRGGGP

QGDKPIFVAKGQKINYAIFWTHRDKEYWGEDAEEFRPERWETTSGGALGKGWEFLPFN

GGPRICLGQQFALTEMGYVITRLLQEYSDISIQPSDAAVKVRHSLTMCSAQGINISLT

RAKEE

 

>CYP52F4 Yarrowia lipolytica gene for ALK4 AB010391

CAG83106.1

MLTNLTIVLITLLVTYTVLTRTALRIQRARKAKQMGATLPPRVN

NGILGWYGLWLVIQNARSMKLPHTLGKRFANGPTWLTPVAGNEPINTIDPENVKAILA

TQFKDFCLGIRHRALSPSIGDGIFTLDGEGWTHSRALLRPQFSRQQISRVHSLERLMQ

ILFKLIRKENGEYFDLQNLFFMFTLDSATEFLYGASVDTLADLLGEPVEGDHGGVGEE

VRKAYQQSINNAQDISAIRTRLQGLYWIAGNIYQRNLYQKSNKGVKDFSQFFVDKALN

TSKEKLKEMEDSDNYVFLYELVKSTRNPVVIRDQLINILVAGRDTTASLLSFTFYTLG

RRPDVLKKLRAAILEDFGTSPDEITFESLKRCDYLRYVLNEVLRLYPSVPINARSATR

DTTLPRGGGPDGKQPVFVYKGQMVAYCVYWMHRDKKYWGEDALEFNPDRWDPKVQPQN

KGWEYLPFNGGPRICLGQQFALTEAGYVVTRMLQEFDTVHCKNQKEEEHPPYALDLTM

RHGEGVWVSMK

 

>CYP52F5 Yarrowia lipolytica gene for ALK5 AB010392

CAG83107.1

MLQLFGVLVLALTTALLAQLAYNKYEYNRKVKQFGCGELTVAKN

GFLGWKGIRAVLHVLKTKKGPAALKERIDAYGRTYVFHIGPAPVISTMEPENIKAMLA

TQFKDFSLGTRYRSLAPTLGDGIFTLDGHGWTHSRALLRPQFAREQVSRLDSLEAHFQ

ILKMCVDKEMREKGNDPRGFDIQNLFFLFTLDSATEFLFGSSVDSLVDFLDDPSVRTG

DHGGVDEAARKGFNNSFNHAQELCALRSRLHTLYWIVGSVVKKEPFERYNKEIKTFVD

FFAAKALKARKEKDMSLMDNDQYIFMYELVKETTNPVTLRDQMLNILLAGRDTTASML

SWIYFRLARDPKLYAKLRSAILEDFGTTPEAITFESLKQCDYLRYVLNEALRLYPVVP

INGRTATRDTTLPRGGGPDQSQPIFIPKGQTVSYSVYWTHRDPRFWGEDAEEFIPERW

DPRNGNIGRGWEYLPFNGGPRICLGQQFALTEVGYVLSRLVQTYETLETCDHKPLPPL

YNHALTMCHEEGVWVKMYKGEKA

 

>CYP52F6 Yarrowia lipolytica gene for ALK6 AB010393

CAG82620.1

MIQSVFLALAILIAYLGFAEWFSRFQHRRISKKKGCGMPPMANG

GFLGWYGLYKTYQITSERTYPHSMRMGLEAFGHTFVYPVPGTDMLQTIHPDNIKAILA

TQFKDFSLGTRHKIMLPTLGDGIFTLDGEGWTHSRALLRPQFARDQVSHVASLERHIQ

VLFKTIKKENKECDPAKGFDIQELFFMLTLDTATEFLCGDSVDSLTDYLADPTAPQLD

HSGIDENVRRAFPEAFNTAQWFCSIRAKLMKLYFFAGTVFYRKKYADANKIVHDFTDF

YVSKALAARKEKFQELDQEGKYIFLYELAKETRNPKVLRDQMLNILLAGRDTTASLLS

WVMFRMARQPETWKKLRQAVINDFGDTPDELSFESLKRCEYLRYVLNEGLRLYPSVPM

NFRVATRDTTLPKGGGPDLDQPIFIPKGGIVVYSVYHTHRAEEYWGKDTEEFIPERWD

PAEGYQIARGWEYLPFNGGPRICLGQQFALTEAGYVLARLAQEFETVTSCDDKPLPPK

YNTHLTMSHDDGVWLKME

 

>CYP52F7 Yarrowia lipolytica gene for ALK7 AB010394

CAG84028.1

MFQLFSILVLAFTTALVAQLAYNQYDYQRKVKKFGCGQLRVAEN

GLFGWKGLREVLRINKYKLGPAALKDRFEKYGKTHVFHVGPSPLITTMDPENIKAMLA

TQFKDFCLIARYKALGPMLGDGIFTLDGHGWTHSRALLRPQFAREQVSRLDSIEHHFQ

ILKKCISKEMSDKRDTQRGFDIQNLFFLMTLDTATEFLFGSSVDSLVDFLDDPSIQTG

DHGGIDEAARKGFSNAFNRAQELSSLRTRLHKLYWVIGTLAVREPYHRYNREVKTFVD

HYAAKAIKARNEKNTDLLDNDKYIFMYELVKETSNPITLRDQMLNILLAGRDTTASML

SWIYFRLARDPKRYAKLRAAVLADFGPGPENITFESLKKCDYLRYVLNESLRVYPVVP

INARTASRDTTLPRGGGPDGSQPIFVPKGQTVSYSVWWTHRDPEFWGQDAEEFIPERW

DTKNGSIGRGWEYLPFNGGPRICLGQQFALTEVGYVLSRMVQTYETLESGDTKPLPPL

YNHALTLCHQEGVWIKTE

 

>CYP52F8 Yarrowia lipolytica gene for ALK8 AB010395

CAG82058.1

MIPFTKINLELLLLPLLIIPITLFLLVLNVQVNTVHRRYKEYRL

KLARCPQQSTGLFGWWLIYAVVRCNRDRFYSAKVVDYFTKLRTFIVSLGGELVIYTSE

SENIKALLATQFSDYDLGKTRHALLFTLMGDGIFTLDGQGWAHSRALLRPQFSKETVS

PLSSLETSLQQLMAIVKRRVALKGEVDIQELFFMLTMDTATNLLYGESVDSLGDCLKE

TKKETHVMTDGKRNLLQSRDHHSNVPNEWEPIYGTYEKKGLGIGSENSLQSGVSPRDS

PVFEVSVSEDIRKAYPAALTTALEFSAMRSKLQRFYWIWGDVLYRQKFHSAVNTVHAF

SNHFVNQALKLTPQELQMKSAEKYTFLYELAQRTRDPVAIRDQLINILIAGRDTTAAL

LSFVFLCLVANPEKLAKLREGIMTDFGTSTDSITFESLKRCVYLRYVINEALRLCPPV

PINMRQANKDTTLPTGGGKNHDEPIFVAKNQIVTYSVLFMHHNQNIWGPDASEFRPER

WGEPACPKGWEYLPFNGGPRICLGQQYALTEAAYVIVRLVQEFTEIEWRDRKGLPVLF

KTHITMSLGEGLRVHMK

 

>CYP52F9 Yarrowia lipolytica

64% to CYP52F3

CAG84211.1

MLEILIGVTLFFTLWRVLVLADLKRRQKKSGYGYPPIASNGLLGWRGLVHQLSGFVKDIGPAGWGAQFKEHGKTHLYPVF

PTQLLVTRDPDNVKAILATQFKDFSFGIRKEALCPSLGYGIFTVEGSSWSHSRALLRPQFSREQISRLDSVEHHFQEFAQ

CVDNFKGEYFDIQKLFFAQSMDTSTDFLLGESVGCLKELLETRDGDESSKKFGANFQHAFDRVQRLVALRVVFQENYWII

GDVFFRNEFRQVNELIQKFVQGYVDKALKARAHKAPIYTNPDKYIFLYELARDTTNPRVLRDQVLNILIAGRDTTAATLS

WLMFELAQKPHIFHKLRKAVIEDFGTTIENISFESLKQCEYLRYVLNEVLRLHPVVPINLRVALKDTTLPRGGGPNGDEP

IFVPKGQKINYAVYWTHRDEQYWGEDAEEFKPERWDTTNHPGPLGKGWEFLPFNGGPRICLGQQFALTEMGYILTRLVQE

YSDIGIDEEYHDRPLRVRHSITMSHGDGVHVRLSREGTVVS

 

>CYP52F10 Yarrowia lipolytica

71% to CYP52F1

CAG82791.1

MLGRTLLVSDHCVDITASIFPGSSHPFTTTNPAMNTFHLLIALATFVVVYILSSSFVYQRRTNALEKQWKCGKPLYYASF

PRSLWHFYVFLRESRKHKLLEGFQRSFEAKKPFLTNKVNIFGKDIFNTCDPENLKAMLATQFKDFCLGERHAQLFPVLGD

GIFTLDGNGWQHSRAMLRPQFARDQVSDVHMVETHLKFLTSRITGSKPVDMQELFFNLTLDTATEFLFGQSVGCQIAESD

PGAYSSDMPLDLRKSFQKDFNKAQEHLGERVRLQMLYWLWNPKELQTAGARVHAFVDHYVSKALVEAEEKVDDDKYVFLR

ELARETKDPKVLRDQALNILLAGRDTTASLLSWCFYLMARDDRVWQKLRSEVIEHFGDGENLENITFESLKRCDYLRYVL

NEVLRLYPSVPANMRFATKDTTLPRGGGPVGQDPIVIRKGNVISYHVFTTHRLTQYWGEDAEEFVPERWAEGKARGWEYL

PFNGGPRICLGQQYALTEAGYVLVRLAQMYDTLENADDKPEPPVKLHALTMSHLTGVHVKLYKKN

 

>CYP52F11 Yarrowia lipolytica

69% to CYP52F2

CAG83401.1

MILLYVLAFLVSYLIFTRLRYRYQSYKLAQKWKCSPVRDVSSFPMAFPEFLFFFKSGKNNVLLDEFVNFFTKRQPNLTVG

LVLAGNDVTVTLEPENLKALLATQFKDFCLGVRHNQLAPILGDGIFTLDGQGWQHSRAMLRPQFARDQVSDVDMIERHVQ

NLVRKIPQGDSFDIQGLFFNLTLDTATEFLFGQSVSCQTVDNPAEDDSTVTDMPIPMRKSFQSDFNLAQHHGGYRVRLQM

FYWLWRPQELFTASKSVRKFVDHYVKKALVESEKEKQADDKYVFLTELAREVKSPQVLSDQALNILLAGRDTTASLLSWC

IYLLARHPETWNKLRDEILSTFGSGSDLSLITFESLKRCEYLRFVINETLRLYPSVPVNVRYATRDTTLPRGGGPDESQP

ILIRKNSVLVYSVFATHRLKKFWGEDADEFRPERWGEGISRGWEYLPFNGGPRICLGQQYALTETSYVLTRIVQLFGTLE

NADASPEPPMKLHALTMCHLTGVHVKMSTNNLP

 

>CYP52G1 Aspergillus nidulans AN6057.1 38% to 539A1 46% to 52F1 52 clan

MLFLSVLLAFAAYLLIYQYAMTNWNHARRARLWGCSPLPRYPTDILGLAT

LRESLKADKEKKIPLLLQNRLKRMSAREKRPVTTFVIRQMGLDNIFTCDH

GNVQAILATKFKNFELGVGRRHTLYPMFGVGIFTSDGETWSRSRALLRPQ

FTRDQISDLDLEESHVQQAMRAMNVDPATGWTSSIDIQAIMFRLTIDSAT

EFLFGESAGSQAEALRNGGTLPLNHFSGDFDLGQWYVAQRSRFEKFYWLV

DNRESRAVVKRVHEYVDRFVHAVLTTAEDRIEKSQSSSYVFLEALAASTK

DPIELRSQLLNILLAGRDTTASLLSWSILMLARYPEVFTKLRSVILADFG

SYTSSRDKITFASLKSCRYLQYFLNEVLRLYPAVPINRRVATTATTLPKG

GGPAGDKPIYLRAGQVVTYSPFVTHRRTDLWGEDAEVFNPERWVNKKVGW

EYLPFNGGPRVCIGQQFALTEAGYVIVRLLQRFDAIMDCFPEREIRYGLT

LTLAPADGVFVRLHAAE*

 

>CYP52G2  Aspergillus fumigatus Af293

         GenEMBL XP_755288.1 also EAL93250.1

         60% to 52G1

MGLPLLTIAAVAVALRLLWTLAARWQHAQNARRLGCGSIPLYPSDPLGISVLKETLAADKAKKLLPLVER

RVALMSDREGRYVTTFRFRQMGRENYFTTDPKNIQAILATQFKDFELGAPRRQALHPLLGAGIFSSDGEE

WFHARGLLRPQFTRDQISDLDLEERHVQKAMQAMPVVNGKWTDAVDIQSIFFRLTIDSATEFLFGESVES

QLSALNGGQTPVDTFPYYFDKSQWYAAQRARFEKLYWIVNNKECRHAQNEVHAYVDRVVHKALKAAREGK

LANPNKPSQYVFLHALVSVTQDPIELRSQLLNILLAGRDTTASLLSWTVLMLARHPAEFHKLRQTIVDEF

GTYDQPRNITFAALKSCQYLQYCLNETLRLFPVVPGNRRSATRDTTLPRGGGSDGTQPIYIRKGQTVVYN

VHILHRRKDIWGPDAEEFKPSRWVDRKVGWDYVPFNGGPRICIGQQFALTEAGYVLVRLLQRFDAIEDMQ

PHLEIRHSLNLTSAPADNVTVRLREAA

 

>CYP52G2 Neosartorya fischeri

97% to CYP52G2 Aspergillus fumigatus = ortholog

NFIA_085030

MGLPLLTIAAVAVALRLLWTLVTRWQHAQNARRLGCGSIPLYPSDPLGISVLKEALAADKAKKLLPMVERRVALMSDREG

RYVTTFRFRQMGRENYFTTDPKNIQAILATQFKDFELGAPRRQALHPLLGAGIFSSDGEEWSHARGLLRPQFTRDQISDL

DLEERHVQKAMQAMPVVNGKWTDAVDIQSIFFRLTIDSATEFLFGESVESQLSALNGGQTPVDKFPYYFDKSQWYAAQRA

RFEKLYWIVNNKECRHAENEVHAYVDRVVHKALKAAREGKLANPDKSSQYVFLHALVSVTQDPIELRSQLLNILLAGRDT

TASLLSWTVLMLARHPAEFHKLRQTIVDEFGTYDQPRNITFAALKSCQYLQYCLNETLRLFPVVPGNRRSATRDTTLPRG

GGSDGTQPIYIRKGQPVVYNVHILHRRRDVWGPDAEEFKPSRWVDRKVGWDYVPFNGGPRICIGQQFALTEAGYVLVRLL

QRFDAIEDVQPHLEIRHSLNLTSAPADNVTVRLREAA*

 

>CYP52G3  Aspergillus oryzae

         GenEMBL BAE65166.1, AP007171.1

         58% to CYP52G4, 63% to 52G1

         14 P450 genes and 2 pseudogenes on this contig

MIPLLILAALAVVFRLVWSIFTSLRHAQNARKWHCGAIPTYPGDILGINTLKEVLRADKEKLIPVLSAQR

VETMTAREGRYVSTFRLRQMGRESIFTSDPKNMQAILATQFKEFELGSLRRNSLHPLLGSGIFSTDGEAW

SRSRSLLRPQFTRDQVSDLDLEERHVQKAMAGMLADPATKWTPEIDIQSIFFRLTIDSATEFLFGDSVES

QTAALSGSRIIEDKFPSYFDRGQWYAAQRARFEKLYWIVNNKESRETDRFVHAYVDRFIDAALAAVKEGK

IDPEKRNSDHYVFLHGLTTATQDPVELRSQLLNILLAGRDTTASLLSWCVLLLARHPDIFQKLRNTILAD

FGDYRNPRNITFSSLKSCRYLHYFMNEVLRLYPIVPGNRRVALKDTTLPRGGGPDGSEPVYVRKGQPVVY

SVFVTHRRKDIWGADAEVFNPDRWEDLKVGWEYLPFNGGPRICIGQQFALTEAGYVLVRLLQRFDQIVDA

RPEREIRFNATLTSAPWENVIVRLREGA

 

>CYP52G3  Aspergillus flavus

99% to CYP52G3  Aspergillus oryzae

AFL2G_05445

MIPLLILAALAVVFRLVWSIFTSLRHAQNARKWHCGAIPTYPGDILGINTLKEVLRADKEKLIPVLSAQRVETMTAREGR

YVSTFRLRQMGRESIFTSDPKNMQAILATQFKEFELGSLRRNSLHPLLGSGIFSTDGEAWSRSRSLLRPQFTRDQVSDLD

LEERHVQKAMAGMLADPATKWTPEIDIQSIFFRLTIDSATEFLFGDSVESQTAALSGSRIIEDKFPSYFDRGQWYAAQRA

RFEKLYWIVNNKESRETDRFVHAYVDRFVDAALAAVKEGKIDPEKRNSDHYVFLHGLTTATQDPVELRSQLLNILLAGRD

TTASLLSWCVLLLARHPDIFQKLRNTILADFGDYRNPRNITFSSLKSCRYLHYFMNEVLRLYPIVPGNRRVALKDTTLPR

GGGPDGSEPVYVRKGQPVVYSVFVTHRRKDIWGADAEVFNPDRWEDLKVGWEYLPFNGGPRICIGQQFALTEAGYVLVRL

LQRFDQIVDARPEREIRFNATLTSAPWENVIVRLREGA*

 

>CYP52G4  Aspergillus oryzae

         GenEMBL BAE55312.1

         54% to 52G1

MSDARTTLMETWFTPALVAAIVAARLLFSLYSAWRHAQRARSLNCQEAPLYPSRDPFGVATLLETLRADR

DKFLPKLSQKRVDLISSQQNRYVSTFRVRQAGRENFFTVDPKNIQAMLATQFNDFFLGDMRRNAGAPVIR

SGIFVSDGADWSHSRSLIRPQFTRTQINNLELEERHVQNALRAMPTQSNGWTSEVDIQTILFRLTLDSAT

EFLFGKSCNSQLTALEKDAGEMSDSFLNSFDRCAWYLAARLRFERLYWIVNNKEFRECTRVVHELVDGYV

HAALQRAQQAEKPADPERGSHYVFVDALTATTQDPNQLRDECLNVLIAGCDTTASLLSWNILLLARHPDI

FQRLRKEIIERFGTYSEPRDINFSSLKSCQYLQHFINETLRLHPVVPFNRRCANKDTTLPRGGGKDGNSP

VYLQKGQPVLYSSYVLQRRKDIWGEDAEEFNPDRWYGRKAIWEHIPFSGGPRTCIGQQFAITNTSFVLVR

LLQRFDSIEDVYPEREIRYGVTLTNCPADRVTVRMHQAEL

 

>CYP52G4  Aspergillus flavus

99% to CYP52G4  Aspergillus oryzae

AFL2G_00228

METWFTPALVAAIVAARLLFSLYSAWRHAQRARSLNCQEAPLYPSRDPFGVATLLETLRADRDKFLPKLSQKRVDLISSQ

QNRYVSTFRVRQAGRENFFTVDPKNIQAMLATQFNDFFLGDMRRNAGAPVIRSGIFVSDGADWSHSRSLIRPQFTRTQIN

NLELEERHVQNALRAMPTQSNGWTSEVDIQTILFRLTLDSATEFLFGKSCNSQLTALEKDAGEMSDSFLNSFDRCAWYLA

ARLRFERLYWIVNNKEFRECTRVVHELVDGYVHAALQRAQQAEKPADPERGSHYVFVDALTATTQDPNQLRDECLNVLIA

GRDTTASLLSWNILLLARHPDIFQRLRKEIIERFGTYSEPRDINFSSLKSCQYLQHFINETLRLHPVVPFNRRCANKDTT

LPRGGGKDGNSPVYLQKGQPVLYSSYVLQRRKDIWGEDAEEFNPDRWYGRKAIWEHIPFSGGPRTCIGQQFAITNTSFVL

VRLLQRFDSIEDVYPEREIRYGVTLTNCPADRVTVRMHQAEL*

 

>CYP52G5P old name = CYP-un1 pseudogene Nectria haematococca e_gw1.8.757.1

Necha1/scaffold_8:999970-1000262

PSEUDOGENE very short piece

57% to 538A3, 54% to 584D1, 61% to 52A3, 68% to 52G2

DESLRLYPVEPINARYANKDTTLPRGGGKDGNSPILIPKGSSTAFSVHIIHRRKDIRGPD

ANEFKPERWEGRRVGWEYVP ()

FNGGPRICIG**FALIGASYVTVRLLQRFDKMESLEKDAVVRHNLKLINYVANGVKVRLHAAS

 

>CYP52G6 Aspergillus niger

fgenesh1_pg.C_scaffold_15000225|Aspni1

57% to CYP52G2

MGLATLLLVLVGLTFILRIVSEKWRHRQNAKRLGCQPAPMAPSKDPLAIGDILEIIQADKDKVVPELLESRTNIMRDNNA

GRYVSTFRLKRGLGENLLTFDPENLQAMLAKQFKDFSVGGERLGCMGPLLGKGIFINDGAEWSHSRTMLRPQFTREQISD

LGLEERHVQNAMRAIPAAGSNGWTEVDIQSIYFRLTLDSATELLFGESCYSQLAAMGDAEDRMASGGKVTDFGKNFDRAQ

WYMAQRLRLPRMKFLYDNKEFRTCCQEVHRFVDECVAKALHDHKKKKQQQQLLEEGASEHYIFVHAMAETTKDPIELRSQ

LLNVLLAGRDTTAALLSWTTLLLSRHPEVFHKLREAIIADFGTYENPQNITFATLKACTYLQQVMNETLRLFPPLPTNAR

YATKDTSLPRGGGPDGQSPVYIKKGQAVLYNVHMLHRREDVWGKDAKEFKPERWEARRSGWEYLPFNGGPRICIGQQFAL

TEAGYVLVRMLQRFDGIEDVHADQRIRWGLTLVSAPGDTVTVRLHEASR*

 

>CYP52G7 Aspergillus niger

e_gw1.7.657.1|Aspni1

67% to CYP52G3

MSTLLFVATASPILYLLYVLLSRWQHAQNARKWNCGSVPSYPGDLLGINTLKEALAMDKARQVPTVTRRRVETMSARENR

YTTTFQFRQLGTDVISTCDPKNVQALLATQFKDFELGQSRRNALHWLLGRGIFTADGDHWSRSRALLRPQFTRDQISDLD

LEERHVQVAMKAMPVDATGWTPATDIQTIFFRLTMDTATEFLFGESVQSQAAALSNGAIPQDDFPAQFDRGQWYSAQRAR

FEKLYWIINNKESRAINKAVHAYVDRFVTAALNSDPEKKPTGHYVFLHGLAEATRDPVELRSQLLNILLAGRDTTASLLS

WCVLFLARHPAYFSSLRATILAEFGSYSSPRDITFANLKSCRPLQNFLNEVLRLYPIVPGNRRTAVRNTTLPTGGGPAGT

DPVYVKKGQPVFYSTYVMHRRPDLWGADADEFRPDRWNERKAGWEYLPFNGGPRICIGQQFALTETGYVLVRLLQRFDVI

EGVGKTKEGEINMQMSLTNAPGDNVTVRLHEDASN*

 

>CYP52G8 Aspergillus clavatus

83% to CYP52G2

ACLA_081330

MAFPLVTIAAVAVALRLLWVLVSKWQNAQNARRLGCGSTPLYPSDFIGISVLKEALKADKAKQLLQLIERRVALMSDREG

RYVSTFRLRQIGREMYFTTDPKNIQAVLATQFKDFELGAPRRNAIHSLLGTGIFTSDGEEWSRSRALLRPQFTRGQISDL

ELEERHVQNAMRAMRVVNDKWTDAVDIQSIFFRLTIDSATEFLFGESVESQLSALNGGGTPADQFPYYFDRSQWYCAQRG

RFEKLWWVVNNRESRHAEKEVHAYVDRFAQNAIKTAQEGKLATDDSSRYVFLHALASVTQDPVELRSQLLNILLAGRDTT

ASLLSWTMLLLARHPAEFQKLRQAVVDEFGTYDQPRNLTFGALKSCQPLQHCMNEVLRLFPVVPGNRRIATKDTTLPRGG

GSDGSQPIYVRKGQPVAYSMHILHRRKDIWGPDAEEFKPSRWVDRKVGWDYVPFNGGPRICLGQQFALTEAGYVLVRLLQ

RFDAIEDVQPHLEIRYALNLTSAPADNVTVRLREASK*

 

>CYP52G9 Aspergillus terreus NIH2624

CH476602

62% to CYP52G2

complement(join(1144551..1145711,1145779..1146177))

ATEG_06678

GenPept EAU33222.1

MATLLSISVLAVVVLRLFWTLAAKFNHARKARRWGCAPYPTYPS

DLLGIGLLKETLAADKADVLCPMFERRIAHISAREGRHVATLSWMSLGRETCFTIDPE

NVRAVWATQFKDFVAGDLRWNVSHQLTGKNIASTDGAEWARYRALLRPQFSRSQISDL

DLEERHVQKVMLAIPVINGKWTEPVDIQEIFHRFTIDSSTEFLFGKSVESQISAITGQ

KTAEADFAHHLDKSMEYIGKRARLDKLYWLANNQESRFSESEVHKYVDRYVQDAIKAG

QEGKLQADPERSPQYILLHALTTATQDPIELRNQVLTLLLAGRDTTASLLSWTVLLLA

RHADEFQKLRQAVLEDFGSYDNPHNLTFGALKSCSHLRHCLNESLRLYPVAPFNRRIA

VRDTTLPRGGGSDGRQPIFVRKGQAIMFSAYSMHRRRDIWGPDADDFRPGRWEARKTL

WEYLPFSGGPRICLGQQFALTEAGYVLVRLVQRFDAMEDVNAETEIKQKVTLTSAPAQ

SVTVRLHAPRA*

 

>CYP52G10 Aspergillus terreus

68% to CYP52G4

ATEG_07540.1

MHTLLFVPALVLAGIAVRLVISFALSWRKARNARRLGCEEAPLYPSKDPFGFSNLLETLRADRAKVLPELAERRIDLLSQ

RHGRYISTFRVRQPGKESLFTADPKNIQAMLATQFKDFGLGDGRRNVGGPLVGRGIFTTDGEAWSHSRSLLRPQFTRAQV

SNLDMEERHVQNAMRALPVLSNGWTRAVDIQTIFFRLTLDSATEFLFGKSCDSQLAAVRTDVGRGSDSFLYGFDRSVWYL

SERLRFDRFYWLVDNREFRESIRMVHAFVDKYVNAVLERQAGDTKLPDSSQSTSQYVFLEALAASSKDPLQLRYESLNVL

LAGRDTTASLLSWAVLILARRPDIFSRLRKDIVEQFGTYKTPRGITFASLKSCQYLQHFINETLRLYPVVPFNRRCALRD

TTLPSGGGKDGSSPIYIPKGQQVLYGSYVLQRRKDIWGDDAEAFNPDRWIGRKVTWEYIPFNGGPRTCIGQQFALIKTSY

VLVRLLQRFDSIVDVHADQEIRYGVALTCAPADLVTVKLHEADD

 

>CYP52H1 Aspergillus nidulans AN7131.1 45% to 584B1 52 clan

MWSPFLEVAKALREGHVVEFITQKYNEYGWTFEQNVLGRSGISTIEPENL

KALLATQFNDFCLGTREREFGPLLGQGIFTLDGAGWSHSRALLRPQFTRD

QVADLDLMESHISRLIELVPKDGSAFDIQRLFFLMTLDSSTHFLFGESVH

CMDEGNVLARSTVNNAQGFANAFNTALDYLNWRSVAGNFYWMITSKEFRD

ANKRVHEVVDYYVHQAIEAKRHPEKKEPGRYIFAEALAADNDNPKVLRDN

MLNILLAGRDTTASLLSSAFFYLSRHPAVWEKLRRVIIEEFGDVQNPKGE

ITHAKLKDLPYLRYVLNEVLRLQPPVPLNLRVAVKDTSLPVGGGPDGKSP

VFVEKDQPVLYSVYAMHRRKDLWGPDADSFRPERWEENAKHGWEYLPFNG

GPRICLGQQYALTEASYTMVRLMQRFSKVENGEPGLDEPLIRATLTMSHE

NGVKVRFKLQHVRRKPSPLQTCTR*

 

>CYP52H2  Aspergillus fumigatus Af293

         GenEMBL XP_746567.1 also EAL84529.1

         cytochrome P450 alkane hydroxylase

         72% to 52H1

MLAPAVALFAAVYTTFLVLRYFQQLYKHRKQARSLRCQPPADGEAGFLGIASFLRLRKAAKEKRWIELIA

EQYGKYGNTFTQTIFGQPLVSTIEPENLKALLATQFNDFELGTRHREFYPLLGDGIFTLDGAGWSHARAL

LRPQFTRDQVADLDLMDGHVSRLIDLVPKDGSAFDIQRLFFLLTIDSATHFLFGESVGSLHAGTDTGLLG

RSAVGNAEGFAEAFNKAQEYLAARSRAVVFYWMINPKEFRDANKRVHEVVDHYVQLALESRRNPEKKQPG

GRYIFAEALAAENDNPKVLRDNMLNILLAGRDTTASLLSSTFFYLARHPNVWIKLRQVIVDAFGDAEHPK

EEITQTKLKDIPYLRYVLNEVLRLLPPVPANFRVANKDTTLPVGGGPDQKSPVYIRKGTIVTYSVYAMHR

RTDFYGPDANEFRPERWEENGKRGWEYLPFNGGPRICLGQQYALTEASFTIVKLLQRFDRIENGDPDLIE

PIQQTNLTLAHDRGVYIRLYSSKAL

 

>CYP52H2 Neosartorya fischeri

95% to CYP52H2 Aspergillus fumigatus = ortholog

NFIA_029600

MLAPAVALFGAVYATFLVLRYFQQLYKHRKQARSLRCQPPAEGEAGIFGISSFLRLRNAVKEKRWIELIAEQYGKYGNTF

TQTIFGQPLVSTIEPENLKALLATQFNDFELGTRHQEFYPLLGDGIFTLDGAGWSHARALLRPQFTRDQVADLDLMDGHV

SRLIDLVPKDGSTFDIQRLFFLLTIDSATHFLFGESVGSLHAGTDTGLLGRSAVGNAEGFAEAFNKAQEYLAARSRAVIF

YWMINPKEFRDANKRVHEVVDHYVELALESRRNSENKQPGGRYIFAEALAAENDNPKVLRDNMLNILLAGRDTTASLLSS

TFFYLARHPNVWTKLRQAIVDAFGGAEHPKEEITQTKLKDIPYLRYVLNEVLRLLPPVPANFRVANKDTTLPVGGGPDQK

SPVYIRKDTVVTYSVYAMHRRTDFYGPDANEFRPERWEENGKRGWEYLPFNGGPRICLGQQYALTEASFTIVKLLQRFDR

IENADPDLIEPVQQTNLTLAHDRGVYIRLYSSKAL*

 

>CYP52H3  Aspergillus oryzae

         GenEMBL BAE64862.1, AP007171.1

         71% to 52H1, 48% to 584G1

         14 P450 genes and 2 pseudogenes on this contig

MLAQIAPLFGALCITFLVLQYLQRLYQQRKRAQSLGCQPAAQGPSGIFGIRSFVRLLNEVRHKRWVEYIA

GQYGRYGNTYTQKALGKWMVS TIEPENIKALLATQFNDFGLGTRHREFYPLLGDGIFTLDGPGWSHARGL

LRPQFTRDQVADLELMDGHISRMIDLIPQDGSSFDIQRLFFLLTIDSATHFLFGESVGALESSNSASLLG

RSSVGSAQGFAEAFGTAQDYLTTRSRAMHFYWMVNPKEFREANQRVHEVVDHYVQLAIQSKNNPDKKSDR

YIFAEALAADNDDPKVLRDNMLNILVAGRDTTASLLSSAFFYLSRNQDVWKKLRQTIIDEFGDSQNPKGE

ITQAKLKDIPYLRYVLNEVLRLLPPVPLNFRVAAKDTSLPVGGGPDGRSPVFIPKGQVVAYSVYAMHRRT

DLYGPDSHSFRPERWEENGRRGWDYLPFNGGPRICLGQQYALTEASYTLVKLVQRFDTLECADPELKQPA

ILSTLTMSHDRGVKVRLSSSVPKCWFNQ

 

>CYP52H3  Aspergillus flavus

100% to CYP52H3  Aspergillus oryzae

AFL2G_05134

MLAQIAPLFGALCITFLVLQYLQRLYQQRKRAQSLGCQPAAQGPSGIFGIRSFVRLLNEVRHKRWVEYIAGQYGRYGNTY

TQKALGKWMVSTIEPENIKALLATQFNDFGLGTRHREFYPLLGDGIFTLDGPGWSHARGLLRPQFTRDQVADLELMDGHI

SRMIDLIPQDGSSFDIQRLFFLLTIDSATHFLFGESVGALESSNSASLLGRSSVGSAQGFAEAFGTAQDYLTTRSRAMHF

YWMVNPKEFREANQRVHEVVDHYVQLAIQSKNNPDKKSDRYIFAEALAADNDDPKVLRDNMLNILVAGRDTTASLLSSAF

FYLSRNQDVWKKLRQTIIDEFGDSQNPKGEITQAKLKDIPYLRYVLNEVLRLLPPVPLNFRVAAKDTSLPVGGGPDGRSP

VFIPKGQVVAYSVYAMHRRTDLYGPDSHSFRPERWEENGRRGWDYLPFNGGPRICLGQQYALTEASYTLVKLVQRFDTLE

CADPELKQPAILSTLTMSHDRGVKVRLSSSVPKC*

 

>CYP52H4 Aspergillus niger

estExt_GeneWisePlus.C_80246|Aspni1

73% to CYP52H2

MLAPAIVFLGAIYLTFRILSSLHQAYHHRKKAKALGCQPPNSVDTGILGITGFYRIAKAAREKRWVEYIAEHYQTSGPTF

RQRALGGLMVTNTVEPENIKALLATQFQDFGLGTRHREFYPLLGNGIFTLDGAGWSHARGMLRPQFTRDQVADLDLMDGH

ITKMMDLIPKDGSTFDIQRLFFLLTIDSATHFLFGESVGSMRTSAESSLLEKSTVGNAQGFAEAFNRAQEYLAARSRAMA

FYWLVNPKEFREANQLVHEVVDHYVRLALEAKRHPEKKEPGRYIFAEALAGDTDDPRVIRDNMLNILLAGRDTTASLLSS

AFFYLARHPNVWTRLRQTIVDEFGDAQHPKGTITHARLKDTPYLRYFLNEVLRLLPPVPLNFRVAAKDTSLPLGGGPDGK

APIYVRKGELVSYSVYAMHRRTDLYGPDAHAFRPERWEENSKRGWEYLPFNGGPRICLGQQYALTEASYTMVKLLQRYNR

IENADPDMVEPIINSSLTLSHDRGVHIRLFSS*

 

>CYP52H5 Aspergillus clavatus

84% to CYP52H2 Aspergillus fumigatus

ACLA_054640

MLAPAVTLLVAVYAAFLILRYFQQLYHHRKQARALRCQPVASGNPGFFGIPAFLRLGQAVKEKRWIEHITEQYGTYGNTF

TQVLLGRRLLSTIEPENLKAMLATQFHDFGLGTRHREFYPLLGDGIFTLDGPGWSHARGLLRPQFTRDQVADLDLMDGHI

SRLIELVPKDGSTFDIQRLFFLLTIDSATHFLFGESVGSLHGGTDTGLLGKSAVGNAQGFAEAFNTAQEYLLTRSRALDF

YWMVNPKEFRDANKRVHEVVDHYVQLALESRRNPEKKKQSGGRYIFAEALAAENDNPKVLRDNMLNILLAGRDTTASLLS

STFFYLARHPNVWTKLRHAIVEEFGDSENPRNEITQTKLKDIPYLRYVLNEVLRLNPPVPANFRVANKDTSLPVGGGPDK

KSPVYVPKGTVVTYSVYAMHRRTDLYGQDADQFRPERWEENGRRGWEYLPFNGGPRICLGQQYALTEASYTVVKLLQRFD

TIENADPGLLVPIQLSNLTMSHDTGVNIRLYSSKGYN*

 

>CYP52H6 Aspergillus terreus

75% to CYP52H5

ATEG_02198.1

MLAPIGLVFGALYLTYFVLQYIQRLYQQRKFAKARQCLPPREGAAGLFGITPFIRLAKAVREKRWVEYIADQYSINGTTF

SQAMLGQRMISTIDPENLKAILATQFNDFSLGTRHREFYPLLGDGIFTLDGAGWSHARGLLRPQFTRDQVADLDLMNGHV

SRLIELVPKDRSTFDIQHLFFLLTIDSATHFLFGESVNSMGSGVLEKSAVGGAQGFAEAFNTAQDYLASRSRAMKFYWMV

NPKEFKDATARVHEVVDHYVRLAIESKNHPERKEAGRYIFAEALAADNDDPKVLRDNMLNILLAGRDTTASLLSSTFFFL

ARSPKVWNRLRETIINEFGDSQNPKSEITHTKLKDIPYLRYVLNEVLRLMPPVPLNFRTATKDTSLPVGGGPDGRSPVFV

PKGTTVTYSVYAMHRRPDFYGPDPNEFRPERWEENGRRGWDYLPFNGGPRICLGQQYALTEASYTLVKLLQHFDTLENAD

PTLLRPVIQSNLTMSHETGVNVRLYRAGTA

 

>CYP52J1P  Aspergillus fumigatus Af293

         GenEMBL XP_746816.1 also EAL84778.1

         alkane hydroxylase predicted

         42% to CYP52F1, in 52 clan

         insertion of 11 C bases causes frameshift

MTNTDIAFGHQHGCELPPELTKNWPLGLDRIKELWTANTEGHLLAFLCSVAEKYEPGNSITQYFLFGPRA

FHILQPESVEAILSTNFKDYGFGARAAIFAPRLGNGIFTQEGPAWRHSRDLLRKQFSRVQNRILEHFHEH

VDNMVARLPLDGVVDLQPLFFNLTLDIATALLFGRSVYSLLAGIDQDADNGLFAEGFNIAQDGLAKRFRI

APWHFLYNPPGFRKACGDVHRFVEQYIDQLDLENSEDLDDKTYGFIKRVAHESASRQHLRDQLLNVLLAG

RDTTACCLSWTFRLLVHHEQAMIRLREEIASVMGDSAHPTKEQIRKMPYLSCVIKE (1)

SLRLYPPP &

PPVPLNNREAIRTTILPTGGGPDADRPILVRKGELVVFFQYVNSRKKNIYGPDADCFRP

ERWETGELDHIGWAYFPFNGGPRQCLGENFALMEVSYTVVRLLQTFSSIILPKGQPIEPVGSERQRLTLV

LSSADGCKVQIHS

 

>CYP52J1 Neosartorya fischeri

95% to CYP52J1 Aspergillus fumigatus = ortholog

NFIA_113870

MQSSQQDNALGHQHGCELPPELTKKWPLGLDRIKELWTSNAEGHLLAFLCSVAEKYEPGNSITQYFLFGPRAFHILQPEN

VEAILSTNFKDYGFGARAAIFAPLLGNGIFTQEGPAWRHSRDLLRKQFSRVQNRNLEHFHEHVDNMVARLPLDGVVDLQP

LFFNLTLDIATALLFGRSVYSLKAGIDQDADNRLFAESFNIAQEGLAKRFRIAPWHFLYNPPGFRKACGDVHRFVEQYID

QLDLENSEDLDDKTYGFIKRVAQESASQQHLRDQLLNVLLAGRDTTACCLSWTFRLLVRHEQVMIRLREEIASVMGDSAH

PTKEQIRKMPYLSCVIKESLRLYPPVPLNNREAIRTTILPTGGGPDADRPILVRKGELVVFSQYVNSRKKNIYGPDADCF

RPERWETGELDHIGWAYFPFNGGPRQCLGEDFALMEVSYTVVRLLQTFSSIILPKGEPIEPVGSERQRLTLVLSSADGCR

VQIHSY*

 

>CYP52J2 Uncinocarpus reesii

71% to 52J1 in overlapping parts

UREG_00942.1

MATFLLIQYVACIVFAYVGYCAIVRFREYCSDTAFGRRLGCQLPPELTKRLPFGIDRIKDLWETDSKGKLLAYLCDVAEQ

YEPGNNLTQYLLVGPRAFHVLHPANLEAVLSTNFSDYGFGARRAIFAPLLGNGISTQEGHAWRHSRELLRKQFIRVQYQN

LDHFREHVDNLIACLPKDGVIDLQPLFFNLTLDVATHLLFGRSVYSLRAGIDQDSKNKVFAESFNVALKGLARRFRSAPF

HFLYNPSKFRKACANVHHFVEQYIDELDIEETEAKDDSSYMFFRQVARESATKEDLRDQLLNVLFAGRDTAACCLSWTLR

LLIRHPYEMERLRAEVASVMRESSHPTRQQIRKMPFLACVIKESLRLYPPVPLNNREAVRTTVLPTGGGSDGQSPILVRK

GELVVFSQYVNSRKKNIWGPDAYEFHPGRWEENKLSDIGWAYFPFSGGPRRCLGEDFALMEVSYTLVRLLQTFPSIVLPD

DEPVEPVGSERQRLTLVLSSANGCRAHVQSA

 

>CYP52K1  Aspergillus oryzae

         GenEMBL BAE66393.1

         44% to 52A5, 44% TO CYP52J1

MLTLSFSALLGIALAWAIHRLAWKYSRLKAICVKRDNKLATQYGCEQPPRLRNWWPLGIDRLIQIWTADS

EQRLMDLFTFPFKDVGYTLEQKFLGTIAFGTIDPENLEAIMTNIN (0)

VFSFGLRRHILFPLLGDGIFTQEGK

SWRHSRELLRPQFTRQHYRDLNIFRPHVDR

LLHYLTGNGAATDLQPLFFRLTLDTTTEYLFGKSVNSLMP

GEATKGQSFANHFDMAQNYVVQRFRLLDLYWLIGGPKFWRSCSAVHRFIDEIIDTRMDSKEKDKDCNSKS

IFFDAVARDSRTRRDLRDQLTNVLLAGRDTTACLLSWTFHCLARHPDVLGRLNVEIASVVGTNTDLTRDD

FKRMPYLDQVLREVLRLYPPVPVNTRTAHKTTILPT

GGGKDGTKPFMVREGENVAFCVYAMHRREDLYGP

DAGQFRPERWDEDLPLFQNERTATWGYLPFNGGPRACLG

QDFGFVEAAYTVVRILQKYPIVKPDMYKGDI

QGRKWLGWSSHQPEGIEMVTEERQKMTIVLSLGDGCRVTLGR

 

>CYP52K1P  Aspergillus flavus

97% to CYP52K1  Aspergillus oryzae

small 6 aa deletion and frameshift = &

AFL2G_11704

MLTLSFSALLGIALAWAIHRLAWKYSRLK (0)

AICVKRDNKLATQYGCEQPPRLRNWWPLGIDRLIQIWTADSEQRLMDLFTFPFKDV

GYTLEQKFLGTIAFGTIDPENLEAIMTNIN (0)

VFSFGLRRHILFPLLGDGIFTQEGK

SWRHSRELLRPQFTRQHYRDLNIFRPHVDR

LLHYLTGNGAATDLQPLFFRLTL

DTTTEYLFGKSVNSLMPGEATKGQSFANHFDMAQNYVVQRFRLLDLYWLIGGPKFWRSCSAVHRFIDEIIDARMDSTEKD

KDCNSKYIFFDAVARDSRTRRDLRDQLTNVLLAGRDTTACLLSWTFHCLARHPDVLGRLNVEIASVVGTNTDLTRDDFKR

MPYLDQVLRE

VLRLYPPVPVNTRTAHKTTILPT

GGAKDGTKPFMVREGENVAFCVYAMHRREDLYGPDAGQFRPERWDEDLPLFQNERTATWGYLPFNGGPRACLG (1)

QDFGFVEAAYT &

KYPNVKPDMYKGDIQGRKWLGWSSHQPEGIEMVTEEGQKMTIVLSLGDGCRVTLGR

 

>CYP52L1   Graphium sp. ATCC 58400 AY438638.1

MMDPFYLLGGCCVFGIVYKSVATLYVNYVHGKTARELGCKPLPK

ERTRFFGLDILRREMKADREMYLPSDIMTRFEEVGATTYEYQMLNEKHIATCDPLVIQ

TVLATQFKEFTFGNRPWGTVLGNGIFSIDNIGDNKEWLHTRAMLKPQFLRNQVSDLEL

EEGHVRNFMSVLKVGDDGWTPKVNLQHMFFNFTLDSATEFLFGKSADCQLLFAPGGDP

KGVSKPLRAFLKALDVAKETVNTKWFLGNNGWIADSPTFRKNCAYVNDFMDALIAKTK

AELEAKPKGSKPSGGREKYHFLHAMLDETDDKVELRGQALNILLAGRETTASLLGWLW

YYLARHPIEFQKLRAAVLKDFGTAENPKPMSFESLKACDQLQYCNNEILRLFPLVSYN

SRMALTDTTLPRGGGPDGNSPIFVKKGQQIVYHPHAMHRRKDIWGQDADEFRPDRWKT

LRPGWEYIPFNGGGRICMGQQFALTEASYLTVRLLQRFDRIENLDPNPVLKQKFRIVN

TPGEGVLVRLHEAAAGA

 

>CYP52M1 Candida bombicola EU552419

alternative name Starmerella bombicola

MLIKDIILTPMSLSAVAGLLPLLFVAFLVLHEPIWLLWYRYAARRHKCSMPRFIEKSFPLGIQRTMDMIKTAKSYTLLEVQYDRVFNKFKARTYLXQAPLQYQIFTIEPENIKTILATKFNDFGLGARFHTVGKVFGQGIFTLSGNGWKQSRSMLRPQFTKDQVCRIDQISSHAAELIKEMNRAMKVDQFIDAQHYFHKLTLDTATEFLFGESCESLNPENQSCIVARDGSEITAEQFVESYNFLLNYAFKRTLSSKVYWLFNSKEFRDHKKRAQSYIDYYVDKALYATSFAAENSIAEKDAAAESSGIYVFSLEMAKVTRDPVTIRDQIFNILIAGRDTTAATLSFAIHFLARNPDVFNKLREEVLDHFGTKEEQRPLSFELLKQAPYLKQVINEVLRLAPVLPLNFRTAVRDTTLPIGGGPEQKDPIFVPKGTAVYYSIYMVHRDIKYWGPDAHEFNPNRWENLKLDNVWAFLPFNGGPRICLGQQFALTELSLTLVRLLQEYSKIEMGPDFPESPRFSTTLTAQHAPPGVVVRFS

 

>CYP52N1 Candida bombicola EU552421

alternative name Starmerella bombicola

MILYAVLGAFAAFLLYMDVLYPFVIYPLRARWHKCGYIPRDLSWPLGIPLTLVVLSKLRKDMLLQFMAAQDLSRPYKTSLRQFLGKWVIATRDPENIKAVLSTKFNDFSLKERGNRMRHVIGDGIFTQDGAPWKHSRDMLRPQFTKDQISRVELLSHHIDVLIREIRKSGGNVELQRLFHLMTMDTATHFLFGESVGSLEVSGESKGIEITDPKTGEIVNTVDFVESYTFANKFALKKIILNDLEFVADLTEPSYKWHLRRVHTVMDHYVQLALKATEKYDPDDDSEKGEYYFSHELAKLTRDPLSLRDQLFNILIAGRDTTAATLSYAFHYLTKNPAIYAKVREDVLTVFPNGDASLATYEDLRKAKYLQMVIKEVLRLAPAVPLNTRAAVRDTYLPRGGGPAGNLPVFVPKGTAVNYPTYILHRDPDIYGADAYEFNPERWRPENKLPNSPXYSWGYIPFNGGPRICIGQQFALTEIALTMIKLVLEFERLEPADDFEPNLQDKSSLTVMVGGSGVRVKLS

 

>CYP52P1 Aspergillus niger

gw1.4.1682.1|Aspni1

46% to CYP52K1

WPLGLDLLVQAFHYDRRQQILKFFLGVVAESGTTFEQNLLFSRGVDTIDPQNIQALLSTQYADFGLGLRPPTFCPLLGSG

IFTQDGAQWRHSRERLRPQFMTNRFTNFEQIRSAVDNLISSISDDNAVNLQPLFFRLTFETTLFLLFGQHLPSLQLEGIK

NQESDFANAFNLGQEYLAKRGRLGGFYWLLDGRDFREACKTCHEFVDRAVQKALDLTTKKTPGTKTEPYIFIDAFIEETR

NPEVLRDQCLNILLAGRDTTACCLTWTLRLLVQHPPVLSKLRAEISDTVGIGPRSPAPTIIQVKSLPYLSLVIKEVLRLY

PSVPVNSRAATKTTTLPTGGGPTGTAPILVRKGEAVGYCVYAMHRRRDIYGADADKFRPERWENDALKDVGWGYLPFNGG

PRVCLGREFALLEVGYTIMRLLQTFEVIEQAE

 

>CYP52P2 Mgr076 Mycosphaerella graminicola

53% to CYP52P1 A. niger, 47% to 52K1 (missing N-term)

yellow from JGI model e_gw1.8.895.1|Mycgr3

LGLGLLLRTFRVAKEQRLLHFFNDLVRSTGNW

TFEQRLLGISGIDTFEPENVEAVLSTQFEADFDLGERRKVFFALLGDGIFTQDGAAWARTRALLRPAFYQQNTDRMLDEIDGLVGKMLRDVPEGEEVDFQPLFFRLTLETTMSLLFGKRMEGEDEQRKKVEMDDFAAAFDEAQDWLARRGRLGGLYWLIDGPGFRKSCRIVHRFIDAAIEEALRVKDVNEGETEGYSVLGALLPETQDRKVLREQCLNVLLAGRDTTACLLSWTCRLLASHPRTLTTLRQEITQICGTDPSPPSRAQLKRMRYLDAILKEVLRLYSSVPINSRTASRTTTLPTGGGPDRTSPILIRKGQAVAYSPYIMHRREDIFGPDAADFRPERWLENDGRLFAEAGWAYLPFNGGRRVCLGQEFALLEAGSVIVGMVRRWREWKVAGDGEAFPEVGTERQKVTLVVSCAEGCRLEVKS*

 

>CYP52Q1 Aspergillus niger

e_gw1.11.186.1|Aspni1

45% to CYP52H3, 41% to CYP52P1

MPWSAASLSLATFFSALVALQLYWIVSRSYRSRKLSKQWNCEPVCRYPSGILGYKVVLRAKTAAQEGQVGSTIQGVHLQY

GNTLKVNLVGHDVVATCEPENIQAVLASQFSSFGLSEWRYPQYRPLLGRGIFTSDGTAWEHSRKLLRPQFTKNQIPSIEW

FDSHSENLMRSLPPDGHAFDIQPLAFRLALDSATGYLFGESINSLVKPDSNQTGVAETSGSGNEGFAAAFDYAQDILFRR

TLALSYYWLINPSDFRKSTRVVHKFVDYYVDKAIEYRSNIGNYKTGHEQKGEEYCFLHALAAETQDREFLRDQLVNVLLA

SRDDVASVLASTFYLLARDTRAWWKLKQEITDAVDRHASNITLKEIQSLPYLRGVLYEVLRLFPPVPVNARVAHHDTTIP

VGGGPDGRSPVFIPKGQMVAYSVWCLHRRRDLWGADAESFRPERWEGYNPPAWEFVPFNGGPRACLGQQFAMILISQQVF

RLVQHLDSIESAQPGPDMGLNPPLRQTLTMCHEHGVHLRVKRMRI*

 

>CYP52R1 Mgr006 Mycosphaerella graminicola

51% to CYP52G2    Aspergillus fumigatus

MHNVALLALLGLAVLGFYKIVASIALKWQHASKARQLGCQDPVVVPGGGVLGLKHLKNMMAADKAQRFPDFMLDRHEMMSKQTGRVCETFRTDLMGQSVFFTSDPENIKAILATQFQDFDLGPVRRAIMGKVLGDGIFIQDGKKWEHSRAMLRPNFVRDQVSDLDMEEIHVKNLLQVMPAKEAGWTDLVNIQTLFFRLTIDASTEFLFGESVLSQVAASKTDGKPNARDETIFSQNFDRSQYHMARQFRFADNYWLYRSSELDQNAKVVNEFVKTYVEAALAAPEKEKSDKYIFSEALAQQTRDPDEIRAQLLNILLAGRDTTASLLSWFFYEMLRNPAVFEKLRGVITETFGTFDEPTDITFATLKSCQYLQYCINETLRVWPVVPGNGRRSNKDTTLPRGGGPDGKSPIFIPAETGIDYSVHVLHRRKDIWGEDAGVFRPERFQGLRPNWTFIPFNAGPRICIGQQFALTEASYVIVRLLQKYDKIEAAPGELDAPALSNLSLTNCPAIPVTLRLHEAE

 

>CYP52R2 Mycosphaerella fijiensis

60% to CYP52R1 Mycosphaerella graminicola

fgenesh1_pm.C_scaffold_45000005

MQNYILIPLLGVALLTLYKILNIIVTRVQNASRSRQLGCQDPPMFPNCGFLGLKHVKLLQAADEKNMFPDFLEERQDIMN

KETGRVCSTFTSYQLGQTIYFTSDPENIKAILATQFADFDLGPIRHAIMGEVLGEGIFVQDGKPWEHSRAMLRPNFAREQ

ISDLDMEERHVQNLFAALPAQEDGWTAFTNIQQLFFRLTMDASTELLFGESVDSQISENPHITKNKSAAKRHDEAAFAIN

FDQAQLHMAKKFRLVNFHWLHNPKEYQVNNKVVNDFVKYYVDLALQQPSNEKQAEEGAHHGKEKFIFLEALAKETRDPDE

LRGQLLNILLAGRDTTASLLSWLFHLLLRNPHVFSTLRSTIITTFGPYHSPTAPITFSTLKSCQYLQSTLSEVLRLRTIV

PGNSRTANKPTTLPRGGGPDGTSPIFIPAGSPIDYSVHVMHRRKDLWGEDALEFKPERFSGRKSGWEFLPFNGGPRICLG

QQFALTEAGYVVVRVLQRFEGIEGIEGEEEEGAVQTDLGLTSCPKREVRLRLRVAREYV*

 

>CYP52S1 Yarrowia lipolytica

42% to 52H3, 44% to 52F1

CAG81974.1

MLLPLLFAGCVFFFLHQRLNYYRRKQRAYEKWLASGKFGAGQKVADIQVQNNFLRNPFGLVQFVEFLNRTKGAFFVDFVN

QNFQKYGYILPTRTLGKVNIITCDPELIKTVLATNFKDWSIGIRRKAMFPVLGNAIFSVEGDQWTHSRAMLRPQFARDSI

ANVADLERHVTRLINVFRGYGVQSFDCQKYFFQFTLDSASDFLFGESTNSLSVQDDKSESEDTGESFNVLTQQGENAESF

AQAFKVAFTFTAIRLRLQQFYWLLRPSNKKYRDSIDTVHRLVDGYVERALENKEDTDRYIISNQLVKVCDDKKYIRDQLL

GILLAGRNTTAAVLAWIMYESARRPEIWNKMRQEVTDTFSDGPVTSDELRKCVYVRSVINEALRVYPSVPMNQRVAVRDT

FLPTGGGPDGSLPLYCPRGTKVAYSVFALHMREDFYGSDSHIFRPERWNEGVGKGWQYLPFNGGPRICLGQQFALMEASY

TLVRLVQEFDTVELDMEVVDPPPKMSSFSMVHRDGVRIRVK

 

>CYP52T1 Aspergillus terreus

48% to CYP52G2

ATEG_03903.1

MNHPTVLAAILALIYVVYKCVDSISDRRKAKALGCQPVPVLKNRLPLGLDHAYRAFQAIKAGQMPDHQVQVYKEVGRRTF

AGSSLGASYIHTVEPKNIQAILATQFHDFELGDLRRKVFFPLLGNGIFTSDGKAWEHSRAMLRPQFTRGQVADLELEERH

VQELFRLMPTDQSGWTQQINLTPMFFRLTIDSATEFLFGVTVHSQRHTSSQQPATDTDAFPWKDLARHFDESTKHLGVRS

LFQELYFLYNPSSFRNNIKEIHRFADFCIQQGLQRLDQQKSSAAQRYIFLDELLKVTRDPVEVKSQLLNILIAGRDTTAG

LLSWTFWLLAQHPAVYSKLRARVLEDFGPDAGRPDRITFAALKACTYLQHVMSEVLRLFPAVPLNARRAARDTTLPLGGG

PDGKAPVFVKKGHEVGYSVYVTHRLEEYWGPDAARFNPDRWVDRKHGWDYLPFNGGPRICLGQQFALTEAGYVIVRLLQR

FDQVELAEPATAPSHNYGITDAPHDYMIYLHEAGSKGG*

 

>CYP53A1 Aspergillus niger X52521

MLALLLSPYGAYLGLALLVLYYLLPYLKRAHLRDIPAPGLAAFT

NFWLLLQTRRGHRFVVVDNAHKKYGKLVRIAPRHTSIADDGAIQAVYGHGNGFLKSDF

YDAFVSIHRGLFNTRDRAEHTRKRKTVSHTFSMKSIGQFEQYIHGNIELFVKQWNRMA

DTQRNPKTGFASLDALNWFNYLAFDIIGDLAFGAPFGMLDKGKDFAEMRKTPDSPPSY

VQAVEVLNRRGEVSATLGCYPALKPFAKYLPDSFFRDGIQAVEDLAGIAVARVNERLR

PEVMANNTRVDLLARLMEGKDSNGEKLGRAELTAEALTQLIAGSDTTSNTSCAILYWC

MRTPGVIEKLHKALDEAIPQDVDVPTHAMVKDIPYLQWVIWETMRIHSTSAMGLPREI

PAGNPPVTISGHTFYPGDVVSVPSYTIHRSKEIWGPDAEQFVPERWDPARLTPRQKAA

FIPFSTGPRACVGRNVAEMELLVICGTVFRLFEFEMQQEGPMETREGFLRKPLGLQVG

MKRRQPGSA

 

>CYP53A2     Aspergillus parasiticus

            GenEMBL AC005991

            Lewis,J., Kupfer,D., Keller,N. and Roe,B.A.

            Aspergillus parasiticus Cosmid Clone ap0

            Unpublished

            57% to CYP53A3

27896 MLLDLYLVKCLFCPL

27941 VPIVALGLLVAYYVAGYLKQWHLHDLPGPFIAGFSRIWLILQVRQGYRSLVVNDLHRRCG 28120

28121 KIVRLAPNHISVADESAIQAIYGHGNGFLK 28210 intron

28267 SNFYNAFLNVDWSIFTTRSRAEHTRKRKIVSHAFSARSLAQVEQYAHNTMELLVRQWQ 28440

28441 KMVDSQEGLDDPYAVIDARVWCNYLTFDIIGDLAFGAPFGMLERGNAIVSMRKALEDAAV 28620

28621 TLDAVEVLNHRGDVSAALGICPDLIPYAKWLPDLFFRQGAEAIANVADVAGAAVDRRLKM 28800

28801 NTSMTEKRGDLLAHLIDAEDQAGAKLGYRELTGEAVTLIAAGSDTSSSTLCALLYWVSTT 28980

28981 PRVLWKLQNVLDEVIPVDVEVPYLAMVKKI 29070 intron

29117 PSLQWVIWEALRIHSTFGQGLPREVPPERGPVEICSHTFYPGDVLSVPGYTMHHSVDIWG 29296

29297 IDVEDFVPERWDPCRLTQRQKDSFIPFSEGPRACIGRNLAEMEFFVGCATLFRLFEFRVE 29476

29477 GQGPLEVREGWLRKPVSLQVGIRRRYLDVRSS* 29575

 

>CYP53A3 Aspergillus nidulans AN7589.1 64% to 53A4 53 clan

MITDFLTPE

NITPERIALALLGLLAAYYVVPYLQTWRLSDIPAPGLAAWTNFWLLLQTR

LGHRFISVDNAHKKYGKLVRIAPRHISIADDAAIQAVYGHGNGFLKSDFY

DAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSAKSIGQFEQYIHHNIENLV

KQLTRISNLQRNPKNGYATVDALNWFNFVAFDIIGDLAFGAPFGMLDKGQ

DIAEMRKSPDSPPQYVQAVEVLNRRGEVSATLGCYPALKPFAKYLPDRFF

RDGLEAVENLAGIAIACVNERLKPEVMANNTRVDLLARLMEGKDANGNKL

GRAELTAEALTQLIAGSDTTSNTSCAILYYCLRTPGVIDKLHKVLDEAIP

QDVEVPTHAMVKEIPYLQWVIWETMRIHSTSAMGLPREIPEGNPPVEISG

HIFKPGDILSVPTYTIHHSKEIWGADADEFIPERWAPERLTARQKAAFIP

FSTGPRACVGRNVAEMELLVICSTVFRMFDWELQQKGPMETREGFLRKPL

GLTVGVKRRTIV*

 

>CYP53A4 N. crassa AABX01000266.1 cont3.41 NCU01086.1 (version3)

243627 MAIISLLMSPWAPVVLLAGVAFYYLVPYFVTYSALRK

IPSPFPAQFTDLWLLSVCRRGNRYQRVDELHKKLGPVVRIQPNH

VSICDDAAIPTIYGHGNGFLN (2) 243932

243994 DFYDAFVSIRRGLFNTRDRAEHT

RKRKIVSHTFSAKSVQQFEPYMHSNLELFVKQWDSMIKNSKNPDKAAHLD

CLEWFNYLAFDVIGDLSFGQPFGMLSSGADMAEIRSSPDAAPIYAPAIEI

LNRRGEVSATLGIHPALKPFAKYLPDPFFTKGLAAVENLAGIAIACVKSR

LDNPPPVTRKDLLQRLMEGRDEKGEPLGREELTAEALTQLIAGSDTTSNS

SCALLFHAVRTPGVMQKLQAELDANIPPEVDVPTYDMVKELPYLEAVINE

VLRFHSTSGIGLPRQIPHDASQGVHIQGYYLPPGTVLSVPTYSIHHSKEI

WGPDADEFKPERWERLTARQKNAFIPFSHGPRSCVGRNVAEMEMKLIVAT

WARRYEVKLLQDYMDTREGFLRKPLGLKVGLKLRK* 245220

 

>CYP53A5 Magnaporthe grisea MG07977.4  69% to CYP53A4 (split on two contigs)

AACU01000984.1 cont2.1481 runs off end, changed C-term

AACU01000983 cont2.1480 = N-term

 89 MAIVNLVFTPLGLASLGAFMLVAYYVVPYFTTFGHLRSIQPASPLAGFSNLWLLYTSRVG 268

269 KRSLLVDEAHARLGPVLRVQPNHVSIADDEAINIIYGHGNGFLKSSFYDAFVSIRRGLFN 448

449 TRDRAEHTRKRKLISHTFAPKSVGQFEPYIHGNLELFAKKWDELIERTKKSDGWAPVECL 628

629 QWF 634

XXXXXXXXXXXX

2 FGAPFGMLNAGADIAEVRMSVDSEPIYAPAVEILNRRGEVSATLGTLPELKPYAGY

LPDSFFSKGLAAVQNLAGIAIARVKSRLENPPDVNRKDLLARLQEGRDAK

GEPLGFEELTAEALTQLIAGSDTTSNSSCALLYWTARTPGVLAKLQAELD

AAIPDGVFAPAFDMIRNLPYLEAVINETLRIHSTSGIGLPRQIPADSPGV

TIRGQYYPPGTVLSVPTYTIHHSKEIWGPDADEFRPERWIENGGLTDRQK

NAFIPFSYGPRACVGRNVAEMEMKMIAATWARRYDVEVRQDVME

VREGFLRKPLALEIGLKRRS*

 

>CYP53A6 Fusarium graminearum FG08079.1 AACM01000324 FGcontig1.324_scaffold5

MAMFTVLPLIWLAPLGLISLFFYYIIPYFWNYRHLRSIPGPLFARLSNWW

LVYACREKSRWKYVNDAHTRYGPVVRIQPNHVSIANEEVINAIYGHGNGM

LKSSFYDASVITTYSIFTSRDRAEHSRKRKVVSHSFAPQSMRNFEPFIQQ

HLNVFLQKWDAMAANEAKFDGYADVESRVWLNYLVLDIIGDLAFGAPFGV

LAKGSEVVDFETEKGPSSLPVITSLSTRSEIAATVGALPELKPYLKWSPD

PFFRTGFNGMINLRTLGTSRITDRLNNPPGDEREKDLLERVREGRDHKGQ

PFGKGELIAEALTVLIAGTDTTSSTMAALLYHVVRTPGVLKKLQAELDEA

IPADVSIPSFEMVKNLKYLGFVVNEALRHHSTISLGLPRLVPENGNGVTI

AGYHFAPGTVLSIPIYTVHHLKEVWGPDADEFKPERWEDVTQRQKQAFIP

FSHGPRACLGRNLAEMELKVITATWARRYDLIMRDDTMEILEGLARKPEA

VNVGIRRRM*

 

>CYP53A7 Fusarium graminearum FG08086.1 AACM01000324 FGcontig1.324_scaffold5

MAITELLVSPW

APVALVVAFVAWYILPWVSNKDLRGIPAPFPAQFSNLWLLSTCRRGKRYE

IVDQVHKKLGPLVRIAPNHVSVADADAINTIYGHGNGFLKADFYDTFVSI

RRGLFNTRDRAEHSRKRKIVSHTFAPKSVLEFEPYIRQNLEIFVKQWDRI

SSNKERDGYGRVDCLNWFNFLAFDIIADLAFGKPFGMLASGADIAEVKAS

PTSPTIYAPAVEIMNRRGEVSATLGCLPQLKPYAKYLPDPFFSQGLQAVE

NLAGIAIARVSERLERGGDSTRKDLLARLMQGRDEKGEPLGRDELTAEAL

TQLIAGSDTTSNSSCALLYHIVRTPGVMKKVYEEISAVMPDGVDIPDFES

VKHLPYLGYCINETLRIHSPSGIGLPREVPPNHKGVTIHGRYFGPGTVLS

VPTYTIHHSTEIWGPDADDFKPERWETLTDKQKSAFIPFSYGPRSCVGRN

LAEMQMRMIAATWIKRYDVVLRQDVMETREGFLRKPMGLDVGLARR*

 

>CYP53A8 Fusarium graminearum FG10451.1 AACM01000435 FGcontig1.435_scaffold7

MAIVDLLFTWWSLPIAAGLVAASYLYSYFITYGHLRDIPAPFPAQFSNLW

LLYVCRRGERYRVVDQIHKELGPVVRIQPNHTSIADAEAIATIYGHGNGF

LKSEFYDAFVSIRRGLFNTRDRAEHTRKRKLISHVFSAKSISQFEPYIHA

NLELFVKQLDKLVASGQMAKNGKREALMDCLPWFNYLAFDVIGDLAFGVP

FGMLASGADVAEVRDTPDSPPIYASAIEILNRRGEVSATLGCFPQLKPYA

QYLPDPFFSNGLNAVKNLAGIAIARVKNRLDNPPSIERMDLLARLMEGRD

EKGEPLGREELTAEALTQLIAGSDTTSNSSCALLYHVTRTPGVLEKLQSE

LDNAIPSEVSVPTYDMVRDLPYLANVINETLRYHSTSGIGLPRQIPPNSP

GVTIKDHFFPPGSILSVPTYTLHHSKEIWGADADDFRPERWENPTELQKT

AFNPFSHGPRACVGRNVAEMEMKLIAATWARRYVPELRQGVMETREGFLR

KPLGLDIALMMRE*

 

>CYP53A10 Nectria haematococca fgenesh1_pg.scaffold_37000003

Necha1/scaffold_37:6771-8451

59% to fgenesh1_pg.scaffold_12000228,

83% to Fusarium graminearum  CYP53A7

FG08086.1 AACM01000324 FGcontig1.324_scaffold5

Note: this seq has two more introns than the CYP53A8 ortholog

This gene model seems correct DRN 2/4/06

MALINLLISPWAPVALVVLFVGWYLVPYFGANRGLRGIPAPFPAQFSNLWLMSTCRRGKRFEVVDQVHKR

LGTVVRIAPNHVSIADADAINVIYGHGNGFLKS (2)

DFYDPFVSIRRGLFNTRSRAEHSRKRKIVSHTFAPKSVLEFEPYIHQNLDLFVKQWDRASSNPEADGAGRLDCLSW (1)

FNYLAFDVIADLAFGKPF (1)

GMLATGADIAEVK

ASPTSPAIYAPAVEIMNRRGEVSATLGCMPWLKPYAKWLPDPFFSQGLQAVENLAGIAIARVSERLERGA

DTTRKDLLARLMQGRDEKGEPLGRDELTAEALTQLIAGSDTTSNSSCALLYHVVKTPGVLQKLQQEIDEA

TADEGVIPSYESVKHLPYLGMCINETLRHHSPSGIGLPREIPAKSKGVTLHGRYFGPGTVLSVPTYTVHH

STEIWGPDAEEFKPERWENITDKQKIAFIPFSHGPRSCVGRNLAEMQMRLIAATWIKRYNIFLRQEKMET

REGFLRKPLGVEIGVSRR*

 

>CYP53A11 Nectria haematococca fgenesh1_pg.scaffold_12000228

Necha1/scaffold_12:587439-589032

84% to Fusarium graminearum  CYP53A8

FG10451.1 AACM01000435 FGcontig1.435_scaffold7

64% TO 53A1 Aspergillus niger

This gene model seems correct DRN 2/4/06

MAIVDLLLSWWTLPIGVAVLVGTYLYAYFVTYGYLRGIPAPFPAQFSNLWLLYVCRRGERYDVMDKIHKK

MGPVVRIQPNHVSIADDEAIPIIYGHGNGFLKS (2)

EFYDAFVSIRRGLFNTRDRAEHTRKRKLISHTFSTKS

ISQFEPYIHSNLELFVKQLDKLITSGTTKDNQGHQQALIDCLPWFNYLAFDVIGDLAFGAPFGMLANGAD

VAEVRATPESPPIYASAIEILNRRGEVSATLGCFPQLKPYAKWLPDPFFSNGLNAVQNLAGIAIARVKAR

LDNPPPEERMDLLARLMEGRDEKGEPLGREELTAEALTQLIAGSDTTSNSSCALLYHVTRTPGVLEKLQA

ELDASIPSHVSVPTFDMVRDLPYLNCVINETLRYHSTSGIGLPRQVPEGSPGVTIRGHFFPAGSVLSVPT

YSIHHSKEIWGPDADDFKPERWEDVTPRQKNAFIPFSHGPRACVGRNVAEMEMKLIAATWARRYNVELKQ

EIMETREGFLRKPLGLDIALKIR*

 

>CYP53A12 Aspergillus fumigatus Af293

EAL93820 XP_755858 cytochrome P450 benzoate 4-monooxygenase

join(<2165575..2165876,2165928..2166734,

                     2166796..>2167246)

85% to CYP53A1

MITDLLSLQNAGLILLGLIAVYYVIPYLQKWHLHDIPSPRFAAFSNLWLLLQARRGRRFLKVDEAHKKYG

KLVRIAPKHVSIADDAAIQAIYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIG

QFEQYIHQNVELFVQQWTKLAKLNGNPRSGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKGKDIAEMR

KTPDSEPTYVQAVEVLNRRGEVSATLGCLPRLIPYAKYLPDRFFKDGVQAVENLAGIAVARVNERLKPEV

MEKNTRVDLLSRLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTTCAILYWCMSTPGVIPKLQKVLD

EAIPDDVDVPTHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPPGNPPVTISGHTFYPGDVVSVPSYTI

HRSKEIWGPDAEKFVPERWDPARLTARQKAAFIPFSTGPRACVGRNVAEMELLVMTGTIFRLFEFEMQQD

GPMETREGFLRKPLGLIVGMKRRAVHASV

 

>CYP53A12 Neosartorya fischeri

96% to CYP53A12 Aspergillus fumigatus = ortholog

NFIA_090480

MITDLLTPQNTGFILLGLIAAYYIVPYLQKWHLHDIPSPSFAAFSNLWLLLQARRGRRFLKVDEAHKKYGKLVRIAPRQV

SIADDAAIQAIYGHGNGFLKSDFYDAFVSIRRGLFNTRDREEHTRKRKTVSHTFSMKSIGQFEQYIHQNVELFVQQWTKL

AKLNGNPRSGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKSKDIAEMRKAPDSDPTYVQAVEVLNRRGEVSATLGCLP

RLIPYAKYLPDRFFKDGIQAVENLAGIAVARVNERLKPEVMEKNTRVDLLSRLMEGKDSNGNKLGREELTAEALTQLIAG

SDTTSNTTCAILYWCMSTPGVIPKLQKVLDEAIPDDVDVPTHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPPGNPPV

TISGHTFYPGDVVSVPSYTIHRSKEIWGPDAEEFVPERWDPARLTARQKAAFIPFSTGPRACVGRNVAEMELLVMTGTIF

RLFEFEMQQDGPMETREGFLRKPLGLIVGMKRRAVHASV*

 

>CYP53A13  Aspergillus oryzae

          GenEMBL BAE60506.1

          58% TO CYP53A14, 81% to 53A3

MIAELLTPTGAAYVLTAAVIVYYILPYLQLWRLRDIPSPGFAAFSNLWLMLQYRKGNRFVTVDNAHKKYG

KLVRIAPRHVSIADDEAIQAIYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIG

QFEQYIHGNAELFVKQWNRIADTQSNPKTGYATIDALNWFNYLAFDIIGDLAFGAPFGMLEKGQDIAEMR

KSPNDKPSYVQAVEVLNRRGEVSATLGACPSLIPWAKYIPDRFFRDGLEAVENLAGIAVARVNERLRPEV

MANNTRVDLLARLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTSCAILYWCLRTPGVIEKLHKVLD

ESIPKDVDVPVHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPAGNPPVTISGHTFYPGDVVSVPTYTI

HRSKEIWGPDAEQFVPERWDPKRLTARQKAAFIPFSTGPRACVGRNVAEMELLVIVGTVFRLFDFEIQQD

GPMETREGFLRKPLGLMVGMKRRSVAV

 

>CYP53A13 Aspergillus flavus

99% to CYP53A13  Aspergillus oryzae, 1 aa diff, N-term extension deleted

AFL2G_03223

MIAELLTPTGAAYVLTAAVFVYY

ILPYLQLWRLRDIPSPGFAAFSNLWLMLQYRKGNRFVTVDNAHKKYGKLVRIAPRHVSIADDEAIQAIYGHGNGFLKADF

YDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIGQFEQYIHGNAELFVKQWNRIADTQSNPKTGYATIDALNWFNYL

AFDIIGDLAFGAPFGMLEKGQDIAEMRKSPNDKPSYVQAVEVLNRRGEVSATLGACPSLIPWAKYIPDRFFRDGLEAVEN

LAGIAVARVNERLRPEVMANNTRVDLLARLMEGKDSNGNKLGREELTAEALTQLIAGSDTTSNTSCAILYWCLRTPGVIE

KLHKVLDESIPKDVDVPVHAMVKDIPYLQWVIWETMRIHSTSAMGLPREIPAGNPPVTISGHTFYPGDVVSVPTYTIHRS

KEIWGPDAEQFVPERWDPKRLTARQKAAFIPFSTGPRACVGRNVAEMELLVIVGTVFRLFDFEIQQDGPMETREGFLRKP

LGLMVGMKRRSVAV*

 

>CYP53A14  Aspergillus oryzae

          BAE56637.1

          56% to 53A3

MDGDSCPSCCSTCTSLNVFLCPHIPILTLGLLLVYYVTGYLKRWHLRDIPGPFIAGFSRIWLIVQVRQGY

RSLVVHDLHRRYGKIVRLAPNHISIADESAIQAIYGHGNGFLETDFYNAFLNVDWSIFTTRSRAEHTRKR

KIVSHAFSARSLAQVEQHAHNNMEHLVRQWRKMIDSEEGLDDPYAVIDARVWCNYLTFDIIGDLAFGAPF

GMLERENATVSMRKAPENPEVTLDAVEVLNHRGDVSAAFGICPDLIPYAKWLPDPFFRQGAEAIANLAGV

AGAAVDRRLKMDTSMTEKRGDLLALLIDAEDQAGAKLGHRELTGEAVTLIAAGSDTSSSTLCALLYWVSS

TPRVLWKLQNVLDEVIPVDIEVPYLAMVKKITYLQWVIWEALRIHSTFGQGLPREVPPERGPVEICGHTF

YPGDVLSVPGYTMHHSADIWGIDVEDFVPERWDPRRLTQRQKDSFIPFSEGPRACIGRNLAEMELFVGCA

TLFRLFEFRVEGQGPLKVRERWLRKPVSLQVGIRRRYLDARSS

 

>CYP53A15  Cochliobolus lunatus Nada Krasevec

           EU597483

           65% to CYP53A1

MFLTSLLLTPYTILLLPVLFYLLPYLRNWRIRDIPAPFPAAWTNLWLLYQCRRGRRFLAVHEAHQKLGKLVRIQPNHVSIADADAITQVYGHGNGFLKSEYYDAFVSIRRGLFNTRDRAEHTRKRKTVAHTFSAKSVLQFEQYIHHNLQELQNQWDRRAESVKGGWYEMDALNWFNYLAFDVIGDLAFGEPFGMLKKGRDEAEVARGGKITYAPAIEVLNRRGEVSGTVGIFPAIKPYAKYFPDPFFSQGMKAVENLAGIAIARVNARLEKPSDRVDLLARLMEGRDENGNKLGREELTAEALTQLIAGSDTTSNTSCALLYHCLQHPEVVQKLQNELDAALPNPDAVPSYAQVKDLPYVDAVIKETMRIHSTSSLGLPRVIPPGPGVTILGRHFPQGTVLSVPAYTIHHSTEIWGPDADTFRPERWEKVTEQQKAAFIPFSYGPRACVGRNVAEMELALIVATVFRRYEFELRQGEMETREGFLRKPLALQVGMRKRSFA

 

>CYP53A16 Mgr055 Mycosphaerella graminicola

64% to CYP53A11   Nectria haematococca

MFLSVLFTPWALVVLPVLYFVLPFLRNWSIMDVPGPFLAKFTTLWYMYECRRCRRYLTVHKLHEKYGKFIRVQPNHVSIADPEAIPIVYGHGTGTLKSEYYDAFVSIQRGLFNTRNRAEHTRKRKTVSHTFSAKSVGQFEQYIHHNLELLAKRWDELSEKSSGGYARFDALHWFNYVAFDIIGDLAFGAPFGMLERGADMAEVQLTPDGPPTYAPAIEVLNRRGEVSNAVGCWPQIKPYAKYLPDPFFSKGMAAIANLAGIATARVNARLAAAEKGEINRVDLLARLMEGKDENGEKLGRAELTAEALTQLIAGSDTTSNTSCALLYHCLTKPNVVKKLQAELDEALPSNDVPTFDQVKDLKYLDMVIQETLRIHSTSSQGLPRQIPAGDGLDVAGRHFPQGTVLSVPAYVMHHSKEIWGPDADEFRPERWEKVTERQKSAFIPFSYGPRACVGRNVAEMELALIVSTVFRRYEFELHQDELETREGFLRKPLGLEVGMRKRDH

 

>CYP53A17 Uncinocarpus reesii

74% to CYP53A1   Aspergillus niger

model has some missing seq (added back)

UREG_05168.1

MLLAFLFNPYIIAGFTICYFYIVPYLQRWDLRDIPAPFPASLSHLWLLYQSRKGKRYQAVHNAHGKYGKLVRIQPNHVSV

ADADAIQTIYGHGNGFLKSEYYDAFVSIRRGLFNTRNRAEHTRKRKTVSHTFSAKSIGQFEQYIHANLQLFLQQWTQICD

LQRNPRSGYASIDALNWFNYLAFDIIGDLAFGAPFGMLSKGRDVAEMKKSPNSPASYVPAIQVLNRRGEVSATLGCFPAL

KPFAKYLPDRFFRDGLEAVENLAGIAVARVAERLRPEVMAKNTRVDLLSRLMEGRDETGAKLGREELTAEALTQLIAGSD

TTSNTSCAMLYWVLRTPGVIEKLQEVLDEAIPAHVEVPTFSMVKDIPY (2)

LQWVILETMRIHS

TSSLGLPREIPQGSPPVTIQGHVFHPGTILSV

PAYTIHHSSEIWGPDVEEFVPTRWDPARLTAQQKAAFIPFSHGPRACVGRNVAEMELHCIAATVFKNFEFQLEQNGPMET

SEGFLRKPLGLLVGIKRRQLDPVVN

 

>CYP53A18 Coccidioides immitis

90% to CYP53A17

CIMG_09108.2

MLLSFLFNPYVLAGIFIFVFYIVPYLRLSYLRDIPSPFAAGFSNLWLLYQCRRGKRYQAVHDAHKKYGKLVRIQPDHVSV

ADADAIQTIYGHGNGFLKSEYYDAFVSIRRGLFNTRSRAEHTRKRKTVSHTFSAKSVGQFEQYIHANLQLFFQQWTNISE

VQRNPKSGYASIDALNWFNYLAFDIIGDLAFGAPFGMLSKGRDVAEMRKSPDSPASYVPAIQVLNRRGEVSATLGCFPAL

KPFAKYLPDKFFRDGLEAVEHLAGIAVARVSERLRPEVMAKNTRVDLLSRLMEGRDETGAKLGREELTAEALTQLIAGSD

TTSNTSCAMLYWVLRTPGVIEKLQEALDEAVPAHVNVPSFSMVRDIPYLQWVIWETMRIHSTSSLGLPREIPPNSPPVTI

EGHVFHPGTILSVPAYTIHHSPEIWGPDVEEFVPTRWDPARLTPRQKAAFIPFSYGPRACVGRNVAEMELHCIAATVFKN

FEFRLEQDGPMETSEGFLRKPLGLMVGIRRRQPNLN

 

>CYP53A19 Fusarium oxysporum

90% to CYP53A8

FOXG_04952

MAVVDILFTWWSIPIAACVLIATYLYSYFVTYGHLRDIPAPFPAQFTNLWLLYVCRRGGRYRVVDEIHKRLGPVVRIQPN

HTSIADPDAIATIYGHGNGFLKSDFYDAFVSIRRGLFNTRDRAEHTRKRKLISHVFSAKSISQFEPYIHANLELFVKQLD

KLVASGQTTDKNGKRQALIDCLPWFNYLAFDVIGDLAFGVPFGMLANGADVAEVRETPDSAPIYASAIEILNRRGEVSAT

LGCWPQLKPYAQWLPDPFFSNGLNAVKNLAGIAIARVKARLDNPPSVERKDLLARLMEGRDEKGEPLGREELTAEALTQL

IAGSDTTSNSSCALLYHVTRTPGVLEKLQAELDEAIPADVSVPTYDMVRDLTYLNNVISETLRYHSTSGIGLPRQIPDNS

PGVTIKGHYFPPGSVLSVPTYTLHHSKEIWGSDADDFKPERWDSVNNLQKTAFNPFSHGPRACVGRNVAEMEMKLIAATW

ARRYTPELKQEVMETREGFLRKPLGLDIALKMR*

 

>CYP53A19 Fusarium verticillioides

98% to CYP53A19 Fusarium oxysporum = ortholog

FVEG_03183

MAVVDILFTWWSIPIATGVLIATYLYSYFVTYGHLRDIPAPFPAQFTNLW

LLYVCRRGERYRVVDGIHKRLGPVVRIQPNHTSIADPDAIATIYGHGNGF

LKSDFYDAFVSIRRGLFNTRDRAEHTRKRKLISHVFSAKSISQFEPYIHA

NLELFVKQLDKLVASGQTTDKNGKRQALIDCLPWFNYLAFDVIGDLAFGV

PFGMLANGADVAEVRATPDSAPIYASAIEILNRRGEVSATLGCWPQLKPY

AQWLPDPFFSNGLNAVKNLAGIAIARVKARLDNPPSVERKDLLARLMEGR

DEKGEPLGREELTAEALTQLIAGSDTTSNSSCALLYHVTRTPGVLEKLQA

ELDEAIPADVSVPTYDMVRDLTYLNNVISETLRYHSTSGIGLPRQIPDNS

PGVTIKGHYFPPGSVLSVPTYTLHHSKEIWGPDADDFKPERWDSLNELQK

TAFNPFSHGPRACVGRNVAEMEMKLIAATWARRYTPELKQEVMETREGFL

RKPLGLDIALKMR*

 

>CYP53A20 Fusarium oxysporum

92% to CYP53A7

FOXG_02776

MAITELLISPWAPLALAVALVAWYILPWISNSNLRGIPAPFPAQFTNLWLLSTCRRGKRYEIVDQVHKKLGVLVRIAPNH

VSVADADAINTIYGHGNGFLKA

DFYDTFVSIRRGLFNTRNR

AEHSRKRKLVSHTFAPKSVLEFEPYIRQNLDIFINQWDR

IASNKDADGYGSVDCLNW

FNFLAFDIIADLAFGKPFG

MLSTGADIAEVKASPTSPTIYAPAVEIMNRRGEVSATLGCLPQ

LKPYAKYFPDPFFSQGLQAVENLAGIAIARVSERLERGGDSTRKDLLARLMQGRDEKGEPLGRDELTAEALTQLIAGSDT

TSNSSCALLYHVVRTPGVMQKLYEEISAVVPEDVAIPDYESVKHLPYLGYCINETLRIHSPSGIGLPREIPPNHKGVTLH

GRYFGPGTILSVPTYTIHHSTEIWGPDADEFKPERWENLTDKQKTAFIPFSYGPRSCVGRNLAEMQMRLIATTWIKRYDV

RLRQDIMETREGFLRKPMGLDVGLARR*

 

>CYP53A20 Fusarium verticillioides

97% to CYP53A20 Fusarium oxysporum = ortholog

FVEG_01611

MALTELLISPWAPLALAVALVAWYILPWISNSNLRGIPAPFLAQFSNLWLLSTCR

RGKRYEIVDQVHKKLGVLVRIAPNHVSVADADAINTIYGHGNGFL

KADFYDTFVSIRRGLFNTRDR

AEHSRKRKIVSHTFAPKSVLEFEPYIRQNLDIFINQWDRIASNKEADGYGSVDCLNW(1)

FNFLAFDIIADLAFGKPF (1)

GMLSTGADIAEVKVSPTSPTIYAPAVEIMNRRGEVSATLGC

LPQLKPYAKYLPDPFFSQGLQAVENLAGIAIARVSERLERGGDSTRKDLLARLMQ

GRDEKGEPLGRDELTAEALTQLIAGSDTTSNSSCALLYHVVRTPG

VMQKLYEEISAVVPEDVAIPDYESVKHLPYLGHCINETLRIHSPSGIGLPREIPPNH

KGVTLHGRYFGPGTVLSVPTYTIHHSTEIWGPDADEFKPERWE

SLTDKQKNAFIPFSYGPRSCVGRNLAEMQMRLIAATWIKRYDVRLRQDIMETREGFLRKPMGLDVGLARR*

 

>CYP53A21 Aspergillus clavatus

90% to CYP53A12

ACLA_074210

MITEILTPQNTGYVLLGLLTAYYIIPYLQTWHLHDIPSPGFAAFSNLWLLLQARQGHRFLKVDEAHKKHGKLVRIAPGHI

SIADDGAIQAVYGHGNGFLKADFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSTKSIGQFEQYIHHNIELFVKQWTKL

SKLNGNPRSGYATIDALNWFNFLAFDIIGDLAFGAPFGMLEKGKDFAEMRKTPDSPPTYVEAIEVLNRRGEVSAALGCFP

RLIPYAKWIPDRFFKDGLQAVENLAGIAVARVNERLKPEVMANNTRVDLLSRLMEGKDSNGNKLGREELTAEALTQLIAG

SDTTSNTACAILYWCMQTPGVITKLQKVLDEAIPADVDVPTHSMVKEIPYLQWVIWETMRIHSTSSMGLPREIPPGNPPV

TISGHVFYPGDVVSVPSYTIHRSREIWGPDAEQFVPERWDPARLTPRQKAAFIPFSTGPRACVGRNVAEMELLVMTATVF

RLFEFEMQQDGPMETREGFLRKPLGLIVGMKRRAAHASV*

 

>CYP53A22 Aspergillus terreus

85% to CYP53A12

ATEG_07877.1

MIADLAAINPAYLLLAAVAAYYIVPYLKRWHLRSIPTPSVAGFTNLWLLIQARRGNRFEVVDNLHKKHGKLVRLAPRHVS

IADDAAINAIYGHGNGFLKALSDFYDAFVSIRRGLFNTRDRAEHTRKRKTVSHTFSMKSIGQFEQYIHHNIELFVKQWTR

LSETQGNPRSGYATIDALNWFNFLAFDIIGDLAFGAPFGMLEKGQDIAEMRKSADAAPTYVQAVEVLNRRGEVSATLGTL

PALIPYAKYIPDRFFKDGIQAVENLAGIAIARVNERLRPEVMANNTRVDLLARLMEGKDANGNKLGREELTAEALTQLIA

GSDTTSNTACAILYWCMSTPGVIDKLHKVLDEAIPADVDVPTHSMVKDIPYLQWVIWETMRIHSTSAMGLPREIPAGSPP

INISGHVFYPGDVVSVPSYTIHRSREIWGPDAEKFVPERWDPARLTPRQKAAFIPFSTGPRACVGRNVAEMELLVMAGTV

FRLFDFEMQQKGPMETREGFLRKPLGLIVGMKRRTPA

 

>CYP53A23 Mycosphaerella fijiensis

88% to CYP53A16   Mgr055 Mycosphaerella graminicola

estExt_fgenesh1_pm.C_10348

MFLSFLFTPWALLASPFLFYLLPFLRNWSIRDVPGPFLAKFTTLWYMYECRRCRRYYTVYKLHEKYGKFVRVQPNHVSIA

EPEAIPIIYGHGTGFLKSEYYDAFVSIQRGLFNTRDRAEHTRKRKTVSHTFSAKSVGQFEQYIHHNLELLAKRWDEIAKN

TGAGKYTRFDALHWFNYVAFDIIGDLAFGAPFGMLEKGADIAEVQLNPDGPVTYAPAIEVLNRRGEVSNAVGCWPAIKPY

AKYLPDPFFSKGMEAIANLAGIATARVNQRLAAAERGEIDRVDLLARLMEGKDENGNKLAKAELTAEALTQLIAGSDTTS

NTSCALLFHCLKNPHVVKKLQAELDEALPSDDVPTYEQVKNLQYLDQVISETLRIHSTSSQGLPRVVPPGDGVEVAGRHF

PPGVVLSVPAYVMHHSKEIWGPDADEFRPERWEKVTERQKLAFIPFSYGPRACVGRNVAEMELALIVATVFRRYEFELYQ

DELETREGFLRKPLGLQVGMRKRS*

 

>CYP53B1 Rhodotorula minuta D63703

MGIVQEAAAFVGSLTAVQLAGAFAALVLAFHVVPYILDPHGLRK

YPAAGPFGIAAFTDAWVVWQARRGKRFEALDKAHRKYGKYVRISPRQVSIADPKALDT

IYGHGTGTMKPAFYDAFVGLKNVRGLFNTRDRAEHTRKRKIVSATFAQRNVLEFEPYI

ASVMRQLLNKWDAMCDKAVKDGKGWVTLDTLTWLNFLAFDIIGDLAFGAPFGMVEREA

DICEIEQEDGSIKHLPAVTILNERGEYSNCLGVMPPHWRPFSKYVDPWFSRGHASVIN

LAGMARARVNARLKSGAGDRKDLLARLQEARDESGNPMDIDELTAEALTQLIAGSDTT

SNSSCAIIFYLARNQDAQKKLQAELDKTFKDRGISGVMEYEDIKTLPYLEACINEALR

MHSTSSMGLPRVMPAQGADFQGEHFKEGTEVSVPAYTIHHLESIWGDPFNYRPERWLG

SDAKQLEKSFIPFSIGPRSCVGRNLATMELLVFMSTLFYRYDFKLADENQTKLETSEG

FLRKPLESWIKMRRRSIDA

 

>CYP53B2 Sporobolomyces roseus

57% to CYP53B1   Rhodotorula minuta, estExt_fgenesh1_pg.C_30339

MTQSHVFTDFFEPTTLAVYFLAAPLGAVFLYLFVPHFTSLAPLRRFPGPFWAGYTRLWLARTARVGKRSELVHREHLKHG

KFVRIGPNEVSIADPAALPIVYAHGSGSIKADFYDAFVASPVRGLFNTRNRAEHTRKRKIVSHTFAPKSVREFEPYIAST

VNLLLKKWDQLAAKAQKSPPSGTGGERMKGYAVIDSLDWFNALAFDVIGELAFGTPFGMVERDAADIVTITKEDGTVIHA

GGVQILNMRGEYSATLGCLPPWSRKYMKYIDPWFARGLESVKNLTGIARTRVNDRLEKGALDRKDILSHLQAGRDENGQP

MSKDELTMEALTQLIAGSDTTSNSSCAILFQIVSTPHAHKKLQQELDEAFSGKGMSGVLEYEDVKALPYLGACINEALRR

HSTSGIGLPRIMMDDTEVLGEVFPKGTVLSVPSYTIHWSTEFWGPDAAEFKPERWLESEEKTRQLEKQLNVFSFGPRSCV

GRNVAMIELFCFMATLVYRYDFKLVDPNQKELEVVEGFLRKPTGCQIGFKLRDATQ

 

>CYP53B3 Puccinia graminis f. sp. Tritici

54% to CYP53B2 from LNRYP

PGTG_08085 +  PGTG_08086 revised

MLVIGLIAAFLEYSILLGLVGISCYYLTGYLRNKHQLNRYPGPFLAKFSRLWLGYATRFGNRYQIIHQLHQKHGRFVRIA

PNELSIADPDAVHIVLGHGTGTTKSKF (1)

YDAFVAIHRGLF (0)

NTRDRADHTRKRKIISSTFSQKSILEFEPYIADTLACFLRKIDQVASEP

NLVQLPSHSWDSKHLNERWRIIDILPWFNYLAFDIIGDLAFGERFGMIERGADIAAVEKEGKVIYLPAIQILNERGEFSA

TQGQFDHIKFSKKKKEKKK (0)

YIDPWFSRGAASVQNLTG (0)

IATNQV

NLRISQTGQSRRDLLARLQTGQDADGNPMGKDELIAEALTQLIAGSDTTSNSSCAILWWVVKHPEVHKRLMEELDE

HLGTEEGVISYADCKELKYLNACINETLRIHSTSSIGLPRILPQTVSFKGHILPKGLVCSVPTFEIHHDPDVWGDP (2)

FTFRPERWLEPNAKDREKAFMPFS (2)

CGPRSCIGRNLAMMELY (0)

MITSTIFKRYEFALVDPDLAELETREGFLRKVGFSLQQKT*

 

>CYP53C1     Ustilago maydis

            GenEMBL XM_397620.1

MVETDLVPRIGAAIQWSVESPAHVVITLLGAVVLFHVVPYITNT

ACIKYPGPFFAKFTDFWLLRTALIGHRFEEVHKQHQKYGKFVRIAPNHVSIADHEALQ

PIYGHGTGTLKPAYYDAFVPPRPFPRGLFNTRDRAEHTRKRKIVSHTFAPKTIVAFEP

FIRREVQLLLERWDEFCDKATKDNTEGPRGIKGRAWLDSLMWLNYFAFDTIGALAFGK

TFGMLENGVDQAKVEYEDANGNKQVDYCSAVQIINERGEFSGTMGLAPVWMRPYLIKL

PWFSSRLKSVKKLTGIALARVNDRLQNGSEREDLLAKLQAAKDDRGEPMGKMELTAEA

LTQLIAGSDTTSNTSCAIVYHLATHPDKMRKLQAELDRELEHAEEVPLHADVQELPYL

QAVLSESLRYHSTSAIGLPRVIPAGGATVCGQQFPSGTILSVPAYTLHRDKSVFGADA

EEYNPDRWLAPNAKRDFEKAFIPFSVGPRACVGRNVAMMELSILIAAIFRRYDIVLAE

PDKPLDTFEGFLRKPVKLEVGLKRRN

 

>CYP53C2 Phanerochaete chrysosporium

         AY321584

         55% to CYP53C1

         my Scaffold_164a

MAVIEALTQLDLKSWLLLIPALAIVAHILVWLLDPHGIRSYPGP

LLAKFSDAWLGYVAAQGHRSEVVHDLHKQYGTFVRIAPNHLSIADPDALQVVYGHGTG

TLKSNFYDAFVSIQRGLFNTRSRSEHARKRKIVSHIFSQKSVLEFEPHVRLYVKQLIQ

QWDRLYEAGAKGLSGDDGEGGWRGRNGRVWLDCLPWYNYLAFDIIGDLAFGAPFGMLL

AARDAAPVAVDHEQAMASYGKEKSEVQYIPAVQVINDRGTYSASLGVLPPWMRPIVKL

FPWFRRGQKAVKQLAGIAVAAVAQRLTTPTDRVDLLGKLQEGRDDDGNLMGKEELTAE

ALTQLIAGSDTTSNSSCAITYYLAKYPDAQRKLQQELDEALGSDDEPVSTFDQVKRLP

YLQAVIDEALRIHSTSGIGLPRLVPKGGMTVCGRFFPEGTVLSVPTYTIHRDEEVWGK

DPEVFRPERWFEQDKNAVQKTYNPFSFGPRSCIGRNLANMELLIIVSSILRRYDFVLE

DPDKPFDTMEGFLRKPVECVVGIRRRTL

 

>CYP53D1 Fusarium oxysporum

48% to CYP53A10

FOXG_14732

MSLDSLLLSPWAPLAVLLCVLLFYILPYFYTYRHLRGIPGPLLARFSDLWLLYICRQSKRSYT

VYDLHERLGPVVRIQPNHVSIVDERAINLVYGHGNGLEKS (2)

SWYDSSISLTRSIFTARKRAEHARKRRYIAHSFAPKSSRAAEGPIADKVELLVRKWDEIIDKGPQFD

GFTQLECRRWFTYLSFDITGDLLFSEPFGMLENGSDLVKIDNKPRSYVSMMNSLAQRSAAVATLGVLPWLKPHAHHLPDP

FFHRGMDGLQNLLGVTSAHVKERMAAGQDNHDDWLSLLLRARDDQGELLKFEEIASESLTLFMAAIETVSNTLSAMMYYL

ATSPSSLQKLQAEVDSIDIPGTVPPFTNVRALPYLDAVLNETMCLHSVLGIGLPREVLPGSKGVHLDSFYFPPGTVLSVP

IYAIHRSRDIWGQDANNFRPERWEKLSDRQKTSFIPFGHGPAACVGRNLAEVEMKIIAATLIKRYNFCMMDSEIESTEGT

TRKLIKVNIGIKRRQ*

 

>CYP54A1 N. crassa AABX01000809.1 cont3.282

4170 MALTQLAHSASALLPLVLPSIAAILAFAIFQRYFAPNPLSNLPIV

     GEEYHGYEKKRQAYLTKAKDLYLEGYTK (0)3952

3824 FKHGLFRIVTPNRNSVIVVSPRFLDELKKLPDDVVSFDAANDD (0) 3696

3565 SMHTKYTLIPTHEPILPHTIKTSLTPSLPRLNPQLSEEVQIAFSQ

EIAPLMSSSPSDWAPININSKLLRIVAKVSGRVFIGPELCHDERYLEAAV

GYTVSVMEAQRAVERMNPWVRPIAAWRLKEVRKLAQMERDATAFLRPVVE

ARREKQRKGEEKDNDMLQWLMDSADQGQGKQHGKWGEDTTTTRKLARLQL

AISFAAIHTTTLVTTHAVYSLAADPKLQATLREEIQSVLQEHKGVFNTSA

LQAMKKTDSFLKETMRFHPLGQTSFNRKVLRTFALSNGQVIPKGSTIEVP

NYAVSRDPEAYPNPDVFDPLRFYNLRNEAREKGEAEQAASGQFVSVNKEF

LTFGYGRHACPGRFFAANEIKMILANLVMTYEMGLVEGETERYRDWDIAA

GTIPDPTKDVMFRKL* 2333

 

>CYP54B1 Magnaporthe grisea MG01391.4  45% to CYP54A1 AACU01000043 cont2.256

AI068604 revised to fill in gap

MSTTQLADVVADRLPLIASAAVILGVTFVVQYFFFKDPLANVPFVGTEFG

GEEARREQFLKDAKPFYLEGFRMNKVHKVTSPRTDTTVSIPAEFLEELKR

LPDDTISFGGAVNEVMAGKYTGIDGIEPTAPHLVKSHLTPALAKINPVLTEE

VARTVREELGSLEDW

TEVNIHSKLLRIVAIVSGRIFLGPELCHNEKYIESAIYYTMELNQGRQAI

FRLPAWQRPFRAWFLPEIKNLHKREREFYALIEPIVKARREARRSDPNYQ

APDDMLTWLEESQDKTTRKSIREIAKLQLGLSFAAIHTTTMTTTNIIYDL

AAHPEMIAELRQEASAALAAHDGAFTAPALHTMKKMDSVMKESMRMNPAG

YSAFMRKVLKPFTLSNGQEIPAGVTIEVPGYGVSRDPETFPNADSWDGLR

FFRLRESGETVGSSKQGGGRASAVEVSAQNQFVSVSKSSLGFGYGRHACP

GRFFASNEVKMILARLVLNYDVKLAGGATERYKNIEAAGSCVPDPTKMLL

FKERA*

 

>CYP54C1 Fusarium graminearum FG11536.1 AACM01000473 FGcontig1.473_scaffold10

MGDTQSAFDIEANRWPVLLTALLVCLVAAIAPKVGSVLRFWTIPVVGEEL

GSTEKRRKAYLGGARKLYSDGYQKLTIEQFKNGIFMITTSKSSPTIVISP

DFLPELKKLPDSILSMEAAVDESMETTYTKIETSVPIIPHTIKGHLTPAL

SRLSAIIADEVRQSLELSIPSCDDWTEVNIHHSLLRIVGMVSGRVFIGPE

LCRSEQYLDAAINYTMEVMGAQRAVQNMRPWLRPFLAQSQPEVKRLYQRI

AEAEAFLQPVVESRIEAMADLSYEKPDDFLQWLIDGKDKFPDKNSQNLAK

VQLGLTFAAVHTTTLTATNAFYDLAAMPDLQVELRDEVQEALLQSEGVFT

SNALQGMKKMDSFLKETLRVHPATMASFQRKVLKPFTLSNGQVIPAGLMI

EIPAVAVNSDPYIFPHADEFDPLRFYRIRTQAKDESSVENAAKEQFVSVN

QSSLTFGYGRHACPGRFFAANEIKMILAHALLRYDVKLVGSETRRYPNIE

FAHMSIPDPSRKLLFKAIGF*

 

>CYP54C2 Nectria haematococca fgenesh1_pg.scaffold_20000135

Necha1/scaffold_20:362069-363960

75% to 54C1 small gap after GYQK? (5 aa), this seq looks right,

maybe the 54C seq is too long here. Note LTIEQ can be removed from 54C and

the intron boundary is preserved.

This gene model seems correct

MGNIQSFVDLAVNRWPTLLAIVVVCLTAAFGPTINRTILLRSLPVVGDGNAEKRRMAYLAGARKLYSDGYQK (0)

FKDGVFTITTSR (1)

TSPIVVISPKFLPELHKLPDSVVSMDAAVDE (0)

TMETKYTKIETSVPIIPHTIKGDLTPSL (1)

NRLNPTIAQEVREALQLSIPSCDDWTEVNIHHSLLRIVGMVSGRVFIGPELCKSEKYLDAAINYTME

VMGAQRAVQQMRPWLRPFLASRLPAVKRLDQRIAEAEEFLNPIVKQRAEAIADPSAEKPDDMLEWLMNRK

DKFPDKNSQNLAKVQLGLTFAAIHTTTLTATNA (2)

FYDLAAMPDVQKELRVEIREALSQTSGVFTSQALQNMKKMDSFLKEVLRLHPATM (1)

ASFQRKVLKPFTLSNGQKIPEGVTIEIPAVAISADSSVFPKADKFDPWRFYRLRQQAKDDGSAEKAALHQ

FVSVNQSSLTFGFGRHACPGRFFAANEIKMILANTILQYDIKLVDATERYPNIEFAHM (0)

SIPDPSRKLLFKAIDV*

 

>CYP54C3 Cryphonectria parasitica

CB690595.1 60% to CYP54C2 N-term only, 55% TO 54C1

MATSTTSSLSETLSERFLVIVAAVLACALAYLGPKIISIIRLAALPVIGTE

LGNEEKRRQAYLQGARKLYYAAYDNFKNGLFKGAARLTTSRNANVIVVSPKFLPELNKLP

DSVVSMEAAVDDAMETKYTKIESHVPIIPHTVTGKLTPSLTRLNPTIARETAEALEL

EMPLREFTNWQEVNIHEKLLRIVGMXSGR

 

>CYP54C4 Cryphonectria parasitica

CB686707.1 CB688482.1

55% TO CYP54C1

TRPMPTCEDWTAVKINSVLLRIVAKASGRIFVGPELCHSEEYLEAAIRYTVEVIGAANAV

SNVPPWLRSFKASKLPEVQRLHERRKHAIKFMQPVVESRKDLDQKPDDLLQWLIDNEGNL

GDMSTWKLARTQLALSFAAIHTSTVVSTNVFYTLAVMPNEVILELRDEIRSVLGENNGNFT

SSALQSMKKVDSFIKETMRYYPFANHSFERKVMRTFTLSNGQVIPAGVILECSTAHNQDD

EVFPDASRFDPWRFSKLQEQEKEDGMDGAARHQMVSVTPNHLTFGYGRHACPGRFFAINX

IKMIIGTFLLNYDIKNIDGVK*

 

>CYP54C5 Fusarium oxysporum

82% to CYP54C1

FOXG_01238

MGNAQSPLDFVSNRWPIIVTALFACLVAAIGPRVVNALRLWTIPTIGEELGSTEKRRQAYLAGARKLYSAGYQKLTTFQF

KDGVFLITTSRTSPTIVISPDFLPELKKLPDATLSMEAAVDESMETKYTKIETSVPIIPHTIKGELTPSLSRLSLTIASE

VRDSLNLTMPPCDDWTEVNIHHALLRIVGMVSGRMFIGPELCRSEQYLDAAINYTMEVMGAQRAVQNMRPWLRPFRAQSL

PEVKKLYQRIAEAEAFLEPVVKTRTDAMNDPTYEKPDDFLQWLIDGKDKFPDKNSQNLAKVQLGLTFAAVHTTTLTATNA

FYDLAALPDLQIELRDEVREALAQSGGKFTSNVLQSMKKMDSFLKETLRVHPATMASFQRKVLKPFTLSSGQVIPAGVTI

EIPAVAVSSDSNVFPQADKFDPLRFYKLRTEAKDGGSVEKAANNQFVSVNQSSLTFGYGRHACPGRFFAANEIKMILAHA

LLQYDVKNAGDATERYPNMEFAHMSIPDPSKKLRFKSVEV*

 

>CYP54C5 Fusarium verticillioides

90% to CYP54C5 Fusarium oxysporum = ortholog

FVEG_00258 revised

MGNTQSLLDLVANRWPLPVTALLACVLAAIGPRVANVLRLWSIPTIGEEL

GNTEKRRQAYLGGARKLYSAGYQKLTDDQFKDGVFLITTSRTSLTIVISPDFLPE

LKKLPDATLSMEAAVDESMETKYTKIETSVPIIPHTIKGKLTPSLSRLSL

TIATEVRDSMNLTIPPCDDWTEINIHRALLRIVGMVSGRLFIGPELCRSE

QYLDAAINYTMEVMGAQRAVQNMRPWLRTFRAQSLPEVKKLYQRISEAEA

FLDPVVRRRTDAMNDPSYEKPDDFMQWLIDGKDKFPDKNSQILAKVQLGL

TFAAVHTTTLTATNAFYDLAALPDLQIELREEVREALSQSGGQFTSNALQ

SMKKMDSFLKETLRVHPATMASFQRKVLKPFTLSNGQVIPAGVTIEIPAV

AVSSDSNVFPHAEKFDPLRFYRLRTEAKDGASVEKAANNQFVSVNHSSLT

FGYGRHACPGRFFAANEIKMILAHALLQYDVKNLGDATERYPNMEFAHMVS*

 

>CYP55A1v1 F.oxysporum M63340

MASGAPSFPFSRASGPEPPAEFAKLRATNPVSQVKLFDGSLAWL

VTKHKDVCFVATSEKLSKVRTRQGFPELSASGKQAAKAKPTFVDMDPPEHMHQRSMVE

PTFTPEAVKNLQPYIQRTVDDLLEQMKQKGCANGPVDLVKEFALPVPSYIIYTLLGVP

FNDLEYLTQQNAIRTNGSSTAREASAANQELLDYLAILVEQRLVEPKDDIISKLCTEQ

VKPGNIDKSDAVQIAFLLLVAGNATMVNMIALGVATLAQHPDQLAQLKANPSLAPQ

FVEELCRYHTASALAIKRTAKEDVMIGDK

LVRANEGIIASNQSANRDEEVFENPDEFNMN

RKWPPQDPLGFGFGDHRCIAEHLAKAELTTVFSTLYQKFPDLKVAVPLGKINYTPLNR

DVGIVDLPVIF

 

>CYP55A1v2 Fusarium oxysporum

96% to CYP55A1v1

FOXG_12350

MASDAPSFPFSRASGPEPPAEFAKLRATNPISQVKLFDGSLAWLVTKHKDVCF

VATSDKLSKVRTRQGFPELNAGGKQAAKAKPTFVDMDPPEHMQQRSMVEPTFTPEAVKNLQPYIQKTVDDLLEQMKQKGC

ANGPVDLVKEFALPVPSYIIYTLLGVPFKDLEYLTQQNAIRTNGSSTAREASAANQELLDYLATLVEQRLVEPKDDIISK

LCTEQVKPGNIDKSDAVQIAFLLLVAGNATMVNMIALGVATLAQHPDQLAQLKANPSLAPQVEELCRYHTASALAIKRTAKEDVMIGDK

LVRANEGIIASNQSANRDEEVFENPDEFDMNRKWPTQDPLGFGFGDHRCIAEH

LAKAELTTVFSTLYQKFPDLKVAVPLEKINYTPLDRDVGIVDLPVTF*

 

>CYP55A1 Fusarium verticillioides

96% to CYP55A1v2 Fusarium oxysporum

FVEG_10773

MASDAPSFPFSRAFGPEPPAEFAKLRATNPVSQVKLFDGSLAWLVTKHK

DVCFVATSDKLSKVRTRQGFPELSAGGKQAAKAKPTFVDMDPPEHMHQRSM

VEPTFTSEAVKNLQPYIQKTVDDLLEQMKQKGCANGPVDLVKEFALPVPSY

IIYTLLGVPFKDLEYLTQQNAIRTNGSSTAREASAANQELLDYLATLVE

ERLVEPKDDIISKLCTEQVKPGTIDKSDAVQIAFLLLVAGNATMVNMIAL

GVATLAQHPDQLAQLKADPSLSPQFVEELCRYHTASALAIKRTAKEDVMI

GDKLVRANEGIIASNQSANRDEEVFENPDGFNMNRKWPAQDPLGFGFGDH

RCIAEHLAKAELTTVFSTLYQKFPDLKVAVPLEKINYTPLNRDVGIVDLPVTF*

 

>CYP55A2 Cylindrocarpon tonkinense D78511

 

MASEPPSFPFQRASGMEPPAEFARLRATDPVSKVKLFDGSLAWL

VTKYKDVTFVATDERLSKVRTRPGFPELNAGGKQAAKAKPTFVDMDAPDHMNQRGMVE

SLFTLEHVKKLQPYIQKTVDDLLAAMKKKGCANGPVDLVKEFALPVPSYIIYTILGVP

FNDLDHLTNQNAIRTNGSSTAREASAANQELLDYLASLVEKRLEEPKDDLISKLCTEQ

VKPGNIEKADAVQIAFLLLVAGNATMVNMIALGVVTLFQHPEQLAQLKANPSLAPQFV

EELCRYHTASALAIKRTAKVDLEIGGKHIKANEGIIASNQSANRDADIFENPDEFNMN

RKWPAEDPLGYGFGPHRCIAEHLAKAELTTVFATLFKEFPDLNIAVPFEKINFTPLGG

DVGVVDLPVTF

 

>CYP55A3 Cylindrocarpon tonkinense D78512

 

MHATEDETTTIPRFPFQRASAFEPPAEFARLRANEPISQVELFD

GSLAWLVVKHEDVCRVATDERLSKERTRLGFPELSAGGKAAAKNKPTFVDMDAPAHMN

QRSMVEPFFTEDHVENLRPYIKETVQGLLNDMVANGCEEPVDLIEKFALPVPSYIIYT

ILGVPFEDLEYLTEQNAIRSNGSGTAQEAAAANQQLLKYLAKLVDQRLQEPKDDLIGR

LVDQQLVPGHIEKSDAVQIAFLLLVAGNATMVNMIALGVVTLMQNPSQLEELKADPTL

VPGFVEELCRYHTGSSMAMKRVAKEDMELGGKLIRAGEGIIASNQSANRDEDVFPNPD

VFDMHRDFDSRDGLGFGFGPHRCIAELLAKAELEIVFETLFATLPDLRVSIPLDEIEC

TPRHKDVGIVRLPVKW

 

>CYP55A4      Trichosporon cutaneum (a basidiomycete fungus)

             GenEMBL AB052733

MMLTRLGLRPLNSFTRALSTRAAPKFPFARASGMEPPAEFATLR

                     ATNPVSQVQLYDGSLAWLVTKYEDVIKVATDERLSKVRTRPGFPELSAGGKAAAQAQP

                     TFVDMDAPDHMKQRGLVEAFFTPEYVEGLKPYIQSVIDEALDKMAAKTQPADLVSEFA

                     LIVPSYVIYTILGVPLKDLEFLTLQNAIRTNGSSTAREASAASAGLLEYLGKLVDARM

                     DEPKDDLVSTLCKAVAAGKLDRTSAVQVAFLLLVAGNATMVNMIALGVATLAKYPSQL

                     ELLKADPSLAANMVQELCRYHTASAMALKRVALEDIVLGGQTIKAGEGIIASNYSGNR

                     DADAFKDPDVFDIRRTFDKDPLAFGWGPHRCIAETLAKVELTAVFETLYKRLPNLKPA

                     VPLEDIAYSPLDKDVGIVELPVTW

 

>CYP55A5v1    Aspergillus oryzae

             GenEMBL AB055659

MNSEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHP

WLVVKHKDVCEVLTDPRLSKVRQRDGFPEMSPGGKAAARNRPTFVDMDAPDHMHQRSM

VSAFFNDEYVESRLPFIRDTVQHYLDRLIRAGKDGKEVDLVKHFALPIPSHIIYDILG

IPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSHDVISTLVI

QQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNL

FVEELCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFD

MFRKRGPEEALGFGYGDHRCIAEMLARAELETVFSTLFQTLPSLKLAIPKSEIQWTPP

TRDVGIVGLPVTWDRD

 

>CYP55A5v1  Aspergillus oryzae

           GenEMBL BAC01275.1

           cytochrome P450nor

MNSEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG

FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDEYVESRLPFIRDTVQHYLDRLIRAGKDGKEV

DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH

DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE

LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG

DHRCIAEMLARAELETVFSTLFQTLPSLKLAIPKSEIQWTPPTRDVGIVGLPVTWDRD

 

>CYP55A5v2  Aspergillus oryzae

           GenEMBL BAE61721.1

           6 aa diffs to CYP55A5v1

MQLEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSKVRQRDG

FPEMSPGGKAAARNRPTFVDMDAPDHMHQRSMVSAFFNDAYVESRLPFIRDTVQYYLDRLIRAGEDGKEV

DLVKHFALPIPSHIIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANKEILEYLERLVDKKTTNPSH

DVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIALGVVTLLEHPDQLSRLLEDPSLSNLFVEE

LCRFHTASALATRRVATVDIELRGQKIRAGEGIIASNQAANRDPEVFPDPDTFDMFRKRGPEEALGFGYG

DHRCIAEMLARAELETVFSTLFQTLPSLKLAVPKSEIQWTPPTRDVGIVGLPVTWDRD

 

>CYP55A5 Aspergillus flavus

99% to CYP55A5v2 Aspergillus oryzae, 2 aa diffs

Supercontig 11: 146268-147914 (-) strand

AFL2G_09432

MQLEPVYPRFPFARPSGDEPPAEFHRLLRECPVSRVELWDGSHPWLVVKHKDVCEVLTDPRLSK (0)

VRQRDGFPEMSPGGKAAARNRPTFVDMDAPDHMHQR (2)

SMVSAFFNDAYVESRLPFIRDTVQYYLGRLIRAGEDGKEVDLVKHFALPIPSH (0)

IIYDILGIPIEDFEYLSGCDATRTNGSSTAAAAQAANK (2)

EILEYLERLVDKKTTNPSHDVISTLVIQQLKPGHIEKLDVVQIAFLLLVAGNATVVSMIAL (0)

GVVTLLEHPDQLSRLLEDPSLSNLFVEELCRFHTASALATRRVATVDIELRGQ (0)

KIRAGEGIIASNQAANRDPGVFPDPDTFDMFRK

RGPEEALGFGYGDHRCIAEMLARAELETVF (1)

STLFQTLPSLKLAVPKSEIQWTPPTRDVGIVGLPVTWDRD*

 

>CYP55A6 N. crassa AABX01000354.1 cont3.356 NCU06137.1 (version3)

153215 MGALTENLEFPFRRASGLDPPPEYAWLRKTDPVTQVRLFDGSL

       AWLVTKYHDVCQVATDERLSKERTRPGFPELAAGGKEAAKNRA

       TFVDMDPPKHMQYRGMVQPIFEMEHVKELEPYIQKTVDDLLER

       MKNMGCEGGPVDLVQNFALPVPSY (0) 152757

152692 IIYTILGVPVEDLDFLTQQNSIRTNGSSTAREASAANQ (2) 152579

152519 ELLNYLRRLAEQRLQEPKDDLISKLMTEQVKAGKLDTADAVQM

       AFLLLVAGNATMVNMIAL (0) 152337

152273 GIVTLFLNPSQLEELKRDPSVVPAFVEELSRFHTASAMAIRRV

       AKEDLEIRGKTIKKGEGVIASNLSANRDEEVFKDADKFDMHRE

       WPVKELGFGWGEHRCIAEFLAKAELKAVF (1) 151893

151771 ETIFKKLPDLKIAVPFDEIEYTPLDRDVGIVKLPVTW* 151658

 

>CYP55A7      Trichosporon domesticus SBUG 752 (a basidiomycete fungus)

             GenEMBL AY044231 and AY044232

AEFAKLRATDPVSKIKLFDGSEAWLVTKYRDVCKVATDDRLSKV

RTRPGFPELNAGGKEAAKAKPTFVDMDKPDHMKQRG

 

>CYP55A8 Fusarium graminearum FG11585.1 AACM01000475.1 FGcontig1.475_scaffold11

MSSDPPSFPFQRASGSEPPAEFARLRATEPVSQVELFDGSLAWLMTKYKD

VTFVATSDKLSKVRTRTGFPELTARGKQAAEAKSTFVDMDPPDHMRQRGM

VESLFTPEAVKKLQPYIKKTVDDLLENLKQKGSANKQVDLVKEFALPVPS

IIYTILGVPFDDLQYLTQ

QNAIRTNGSSTAREASAANKELLLYLASLVEKRLEEPKDDLISKLCTEQ

VKPGNIEKPDAVQIAFLLLVAGNATMVNMIALGVVTLAQHPEQLAQLKSN

PSLAPQFIEELCRFHTASALAMKRTAKEDVEIGGKLIKVNEGIIASNQSA

NRDEDIFENANQFNINRKWPAEDPLGFGFGDHRCIAEHLAKAELITVFAS

LYQKFPDLNVVIPLEEVNYTPLSGDVGIVDLPVTL*

 

>CYP55A9 Nectria haematococca

fgenesh1_pg.scaffold_30000098

Necha1/scaffold_30:272059-273639

92% to CYP55A2 Cylindrocarpon  tonkinense D78511

79% to CYP55A8 Fusarium graminearum 

This gene model seems correct DRN 2/5/06

MASEPPSFPFKRASGMEPPAEFARLRASDPVSKVKLFDGSLAWLVTKYKDVTFVATDERLSK (0)

VRTRKGFPELSAGGKQAAKAKPTFVDMDAPDHMKQR (2)

GMVEPLFTRKHVEKLQPYIQKTVDDLLAAMKKKGCATGPVDLVKEFALPVPSY (0)

IIYTILGVPFNDLEYLTNQNAIRTNGSSTAREASAANK (2)

ELLDYLASLVDKRLEAPEDDIISKLCREQVKPGNIEKADAVQIAFLLLVAGNATMVNMIAL (0)

GVVTLFQHPEQLAKLKENPSLAPQFVEELCRYHTASALAIKRTAKVDLEIGGK (0)

HIKANEGIIASNQSANRDADIFENPDEFNMNRRWPAEDALGYGFGAHRCIAEHLAKTELTTVF (1)

ATLFKELPDLKIAVPFEKINFTPLDRDVGVIDLPVTF*

 

>CYP55A10 Nectria haematococca

fgenesh1_pg.scaffold_28000090 [Necha1:94366]

Necha1/scaffold_28:245547-247185

96% to CYP55A3 Cylindrocarpon  tonkinense D78512

Note: the very high similarity suggests a very close relationship  between the

Two species.  This is higher than the ortholog pair matches between

F. graminearum and N. haematococca

This seq is originally of bacterial origin (P450 B type not E type) similar to CYP105s

This gene model seems correct DRN 2/4/06

MHATEDETIIIPRFPFQRASAFEPPAEFARLRANEPISQVELFDGSLAWLVVKHEDVCRVATDERLSK (0)

ERTRLGFPELSAGGKAAAKNKPTFVDMDAPAHMNQR (2)

SMVEPFFTEDHVESLRPYIKETVQGLLNDMVAKGCEDPVDLVEKFALPVPSY (0)

IIYTILGVPFEDLEYLTEQNAIRSNGSGTAQEAAAANQ (2)

QLLKYLAKLVDQRLQEPKDDLIGRLVAQQLVPGHIEKSDAVQIAFLLLVAGNATMVNMIAL (0)

GVVTLMQNPSQLDELKADSTLVPGFVEELCRYHTGSSMAMKRVAKEDMELGGQ (0)

LIRAGEGIIASNQSANRDEDVFPNPDVFDMHRDFDSRDGLGFGFGPHRCIAELLAKAELETVF (1)

ETLFTTLPDLRVSIPLDEIECTPRHKDVGIVRLPVVW*

 

>CYP55A11 Nectria haematococca

estExt_fgenesh1_pg.C_880004 (better model)

Necha1/scaffold_88:11555-13495

69% to AB226066.1  Aspergillus oryzae cDNA

58% to fgenesh1_pg.scaffold_28000090

54% to CYP55A8 Fusarium graminearum

58% to CYP55A3 Cylindrocarpon  tonkinense

same as fgenesh1_pg.scaffold_88000004 which is too long at

SNAQAHPAP between MHQ and RQM

Note: This gene has one extra intron at the N-terminal

that only codes for 3 amino acids

This gene model seems correct DRN 2/5/06

MAS (1)

DPHFPFARQNGAEPAPEFQQRLTSCPVARVELWDKSHPWLVVKHKDVREVLTDPRLSK (0)

NRQKEGFPEMSAGGKAAAKNCPTFVDMDPPDHMHQ R (2)

QMVAAFFTPEYVEQRKPWIKGVVQHYLNEFINARKGQNSIDLVEHFALRVPSH (0)

IIYDILGVPLRDVDYLTKCNATRTNGSATASAAQDANT (2)

ELLDYFAKLIDKKIESPEPSNDVISSLVANQLANGQLDKKDVVQISFLLLVAGNLTMINMIAL (0)

GVITLLDHPDQLDALIRDPSLSKPFVEELCRFHTASSFATRRVAKVDVKIRGQ (0)

EVKAGEGIIASNQAANRDPDVFSDPNVFDMFRKRGSEEALGFGWGDHRCIAESLARAELEAVF (1)

STLFQTLPTLKLAIPNSEIKWTPPIKDIGVSELPVTWSSSHVGGRRSQL*

 

>CYP55A12P Aspergillus niger

gw1.11.1089.1|Aspni1

48% to CYP55A5v1 aa 53-257, this is a pseudogene

LKSIPVSRKNLFDGSCP*MVIKLKETYNILVDPRLLK (0)

VRQRDRYPEISAGAKKRAENSPTFINMDPSVHGHQRFIARLNS* (2)

SFKPEYIKSCEGFNKHTVRRYLERLIKTKADQQSVDRVENFTLSIPSH ()

IIYNLLGIPDKDFIYLTNCNAT*TNSGNTAGAAQSANE (2)

KYLKYFDHLLDERIANPGDNIISSLAKEQLQQGNLER*DII

 

>CYP55A13 Uncinocarpus reesii

71% to CYP55A5v1  Aspergillus oryzae

UREG_06342.1

MATEATPPKFPFARPRGAEPPAEFAKLRATNPVSRVELWDGSHPWLVVKYNDVCSVLTDERLSKQRQRDGFPEMSAGGKA

AAKNRPTFVDMDPPDHMRQRGMVTSFFTPEYTESIKPQIQKTVDRYLEEMIKGGCEKPVDLVEKFALPIPSCIIYDILGI

PFEDSDSLTQWNAIRTNGSSTAAAAATANTELLGYLDKLVDKKIANPQNDLISTLVTEQLQPGNIDKLDVVQISFLLLVA

GNATMVNMINLGVVTLLEHPDQLEDLKRDPSLAKPFVEELCRYHTASALATRRVAKVDITLNGQLIKAGEGIIASNQAAN

RDEDIFPDPDKFDIHRKRGSESALGYGYGDHRCIAEGLSRAELEAVFSTLFQRLPNLRLGIPHSEIKYSEPTMDVGIAEL

PVVW

 

>CYP55A14 Histoplasma capsulatum

73% to CYP55A13

HCB04560.1

MCVCLSARSKSAIPARSAIVARNQRCNMSTEAAPPKFPFSRPSGAVPPLEYAKLRATNPVSRVTLWDNSQPWLVVKHKDI

CSVLTDSRLSKERTRAGFPEMNPGGKEAAKNRPTFVDMDPPDHGRQRNMVASLFTRESSETMRPHIQATADALLDEMIKQ

GCEKPVDLVEKFALPLPSYIIYGILGVPKEDLAYLTECNAVRSNGSSTAAAASNANKELLDYIGNLADKRLEKPGNDMIS

KLVVEQLKPGHIDRLDVIQIAFLMLVAGNATLVNMISLGIVTLLQHPKQLADLKANPSLAPKFVEELCRYHTASAMATRR

VAKEDIMLGGHLIKAGEGIIAATQSGNRDEDIFPNPDVFDMHRTRGSEVALGYGYGEHVCVAEGLARTELEVVFSTLFRR

LPNLKLGIPLSEVKYSPPDADVGITELPVVW

 

>CYP55A15P  Fusarium oxysporum

74% to CYP55A2 Cylindrocarpon tonkinense

FOXG_14888

SNPRSFPFERASVLEPPAEFAKLRATDPVSQVKLFHGSLPWLVTKHKDVVFVATPDKLSK (0)

VCTRPGFPELDAGGKEAAKAKPT (?)

LDMDSPDHMHQR (?)

RRMVEQIFTQEAVEKLQPYIQKTVDDLLEDLKQKGCADGPVHLVKIFALPAPSY (0)

VIYTILGAPFHDLEYLT*QNAIRSNGSSTAREPSAANN (2)

REFLDYVANLADK*LEEPQDDLISKLCTEQVKPGNIEKADAVQISFLLLVAGNATMVNMIAL (0)

GVITLPQHPDQLSELKP*SIPGPQFVEELCRYHTASALAIK

RTAKEDLEISGK (0)

SKGIIASNQSANRHEDAFKNPD*FDMDCKWLVEDALGFGFDGHRCIDEHLAKTEPTTVFGT (?)

LYRKFPDLKNAVPLEEVHYTPLCQDVGVVDLPVTF*

 

>CYP55A16P Fusarium oxysporum

83% to CYP55A11

FOXG_17502

INYDILGVALKDVDYLTQCNATRTNGSATASAAQDANM (2)

ELLNYFAKRVDKKIESPEPSNDVIGSLVSNELAN &

VDKKHVVELSFLLLVAGNLTMINMIALV (1)

CVLTLLEQPDQLDALIQDPSLLKPFVEELCRYHTASSFATRRVAKVDIKVRGH ()

EVKAGEGIIA*NQAANRDPDVVPDPNVFDLF*KRGSEEALGFGWGDHRCIAASLARAEPEAVF (1)

STLFQTLPTLKLAIPSSEINGSAPNKDIGVSELPVTWSSPQVS*

 

>CYP55A17 Aspergillus terreus

73% to CYP55A13 Uncinocarpus reesii

ATEG_06140.1

MTTTRAVSRPSLWAKYRPVPRPRFSYVSFNSLAGFTSRSGPLREAAAGTQ

TDAPEFPFARPRAAEPPAEFAKLRSSCPVS

RVKLWDGSLPWLVVKHEDVCNVLTDTRLSKERSRAGFPEMNPGGKAAAKNRPTFVDMDPPNHMRQR (2)

RSMVSAFFTPEYIDSMKPFIQSTVDNVLNDMIAKGCDEPVDLVERFSLPIPSI (0)

IIYHIIGVPTEDMDYLTQKNAVRSSGSSTAAAAQNANEELLAYLGSLVDKRIADPKKDLISTLITEQ

LNPGHLDRLDVVQLAFLLLVAGNATMVNMINLGVVTLLEHPSQLEELRRDPLLARKFVEELCRFHVASSFATRRVAKVDI

TLRDKHIKAGEGIIASNQSANRDEDVFPDPDTFNMHRETDSEQNLAYGYGDHRCIAEGLARAELEAVF (1)

SCLFRLPNLKLGVPHDQVRYSEAHKDVGIDELPVTW*

 

>CYP55A18 Trichophyton rubrum

ESTs DW700172.1, DW689200.1, DW681558.1

72% to CYP55A14 Histoplasma capsulatum

MFISRPVITSIGSRVSVQLTTTGLRAMATAV

PKFPFSRPRGAEPPAEYARLRATDPVSKVELWDGSQAWLVVKHKDICSVLTDQRLSKQRN

RPGFPELDAGGKEAAKRKPTFVDMDPPEHMQQRSMVEPLFTREHIDGMRPHIQQTVNTLI

DEMIIGGGKPAVDIVEKLALPTASYIIYGILGVPFKDLEYLTQQAAIRSNGSATAAAASA

ANQQLLEYIGGLVDQRIAEPRNDLISKLVVEQLKPGHLQRDDVIQMAFLMLVAGNATMVN

MINLGIVTLFENPSQLADLKKDLSLVPQFVEELCHFHTASAMATRRVAKVDI

ELGGKTIKAGEGIIAATQSGNRDADVFPDPDTFNMHRKRGAESAFGFGYGEHRCVAEWLA

RAELEIVFTTLFRRLPDLRLAVPLDEVKYSDPSKDVGITELPITW*

 

>CYP55C1 Aspergillus niger

fgenesh1_pm.C_scaffold_5000072|Aspni1

40% to CYP55A1, 40% to CYP55B1

MYPFARPPKSYDPPAELAELRHQGPTERVQLFDGRPAWIVTRDKEVREMLSSDKFSNDRYNRDGYPEIHSGTKKDGVRPT

FVHMDDPKHARHRAMTESFFTPEATEAVKPQVQEVVDSLIDSIKERGCNNGPIDLVKELATVVNPKVILLSILKVSEKEA

NELIQSSSALGGTSGSASESGHTDLHEYISQLIDARIEKPRRPQDDLLSKLVVDEYRQANLDRDDLINLVYMIFVAGNSA

IQSSIVLGVITLLQHQDQLEELKENPGLAGRVVEEILRYHTPSALNSRRVTTTDITLGGKKIKSGSGVIGSVRSANRDER

VYPDADKFSIHRQVDPHQNLAFGHGPHNCQGQWLSRMELQAIFSSLFRKLPNLRLAIEPSELEYTSPTQNVGVLRLPVVF*

 

>CYP56A1 S. cerevisiae DIT2 X55713

MELLKLLCLILFLTLSYVAFAIIVPPLNFPKNIPTIPFYVVFLP

VIFPIDQTELYDLYIRESMEKYGAVKFFFGSRWNILVSRSEYLAQIFKDEDTFAKSGN

QKKIPYSALAAYTGDNVISAYGAVWRNYRNAVTNGLQHFDDAPIFKNAKILCTLIKNR

LLEGQTSIPMGPLSQRMALDNISQVALGFDFGALTHEKNAFHEHLIRIKKQIFHPFFL

TFPFLDVLPIPSRKKAFKDVVSFRELLVKRVQDELVNNYKFEQTTFAASDLIRAHNNE

IIDYKQLTDNIVIILVAGHENPQLLFNSSLYLLAKYSNEWQEKLRKEVNGITDPKGLA

DLPLLNAFLFEVVRMYPPLSTIINRCTTKTCKLGAEIVIPKGVYVGYNNFGTSHDPKT

WGTTADDFKPERWGSDIETIRKNWRMAKNRCAVTGFHGGRRACLGEKLALTEIRISLA

EMLKQFRWSLDPEWEEKLTPAGPLCPLNLKLKFNENIME

 

>CYP56A2 Saccharomyces paradoxus NRRL Y-17217

AABY01000059.1

91% to CYP56A1

46799  MELFKLLGLTLFLTISYIAFAIIVPPLNFPKNIPTIPFYVVFLPVfVFPIDQTELYDLYIR  46620

46619  ESMEKYGAVKFFFGSRWNILVSRSEYLAQIFKDEDTFAKSGNQKKIPYSALAAYTGDNVI  46440

46439  SAHGAVWRDYRNAVTNGLQHFDDTPIFKNAKLLCTLIKRRLQEGQLSIQMGPLSQRLALD  46260

46259  NISQVALGFDFGTLKHEKNAFHEHLIRIKKQIFHPFFLTFPFLDLLPIPSRKKAFKDVAS  46080

46079  FRELLVKRVQDELVNTYKFEQTTFAASDLIRAHNNEIIDYRQLTDNIVIILVAGHENPQL  45900

45899  LFSTSLYLLAKYSNVWQERLRQEVNGVTDPKILADLPLLNAFLLEAVRLYPPLSTIINRC  45720

45719  TTRTCKLGPEIVIPKGVYVGYNNFGTSHDPRTWGATADDFKPERWGSDIETIRKNWRMAK  45540

45539  NRCSVTGFHGGRRACLGEKLALTEMRITLAEMLKQFRWGLDPEWEEKLTPAGPLCPLNLK  45360

45359  LKFTENITE*  45330

 

>CYP56A3 Saccharomyces bayanus MCYC 623

AACA01000139.1  Sbay_5318

83% to CYP56A1

23165  MSILQLIGFILLIIISYIAFVVVVPPLNFPRNIPTIPFYVVFLPVIFPIDQTELYDLYIR  22986

22985  EPMEKYGAVKIFFGSRWNILVSRSEYLAQIFKDEDTFAKSGNQKKIPYSALAAYTGDNVI  22806

22805  SAHGKVWRDYRNAVTNGLQHFDDAPISKNAKTFCNLIKKRAQNGESSIQMGPLSQRLALD  22626

22625  NISQVALGFDFATLTQDKNALHELLVHIKKQIFHPFFLAFPFLDLLPIPSRRKAFRDVAS  22446

22445  FRELLVKRVQDQLINNYKFEQTTFAASDLIRAHNNEVIDYKQLSDNIVIILVAGHENPQL  22266

22265  LFNTSLYLLAKYSTEWQNSLRQEVESVTNSKALADLPLLNAFLFEAVRLYPPLSTIINRC  22086

22085  TTKLCKLGADIVIPKDVYVGYNNFGTSHDPKNWGPTANDFRPERWGSDIETIRKNWRIAK  21906

21905  NRCTVTGFHGGRRACLGEKLALTEMRITLAEMLRQLEWTLDPEWEEKLTPAGPLCPFNLK  21726

21725  LNFKERLPS*  21696

 

>CYP56A4 Saccharomyces castellii NRRL Y-12630

AACF01000075.1 Scas_Contig632.25

63% to CYP56A1

43945  MPALRVILSLLIVLVGYVIFKVVWPPLNFPRNIPTIPCYVVFLPIIFDIDQVDIYNTYIK  43766

43765  VQMEKYGAVKIFFGSRWNILVSKPEYLAQIFKEEDVFAKSGNQKKIPYSAIAAYTGDNII  43586

43585  SAHGDVWRKYRSIMTNGLQHFKEEPFICNAKKFCGLLRKNRGPTGNVLMGPLIQRLTL  43412

43411  DNISQVVLGFNFGTLSEETNELHQHLIKIKKQIFHPFFLTFPFFDMLPIPSRKKAFQDIS  43232

43231  KFREVLVSRVKNDLINNYKFEQTSYVSSDLIKAYNNNIIDYKQLTDNIVILLVAGHENPQ  43052

43051  LLLSNCLYLLAKYPSWQKLIWKETQNISNYKDLNELPLFNSFLFEVVRLYPPLNIIINR  42875

42874  KTNKKCRMGQDIIVPKNTYVGYHNFGVTHNRNVWGNTAEEFDPRRWGTNIADIMIAWRSS  42695

42694  KNSCKLTSFHGGRRACLGEKLGFVEMRISIFEIIQQFELTLPKEWKEKMTPAGPLCPYNL  42515

42514  QLNIKSREKDVGVCE*42467

 

>CYP56A5 Candida glabrata strain CBS138

CR380952.1, CAG59007.1

57% to CYP56A1

MLNILVFGFICLVLCWILTICFPPISFPNNIPTIPFYSVFIPQY

YNIDQVQFYNLYIREKLEKYGAVKFYFGSRWNILVSRPEFLNNIFKNEDTFAKSGNQQ

KIPYSVLAAYTGDNVISAHGVNWQKYRKPIKDGLQHFDIGILLKNAMKFCELIKEDMR

PFKYHCNFKIIPYIQRLTLDNICRVGLGFEFGAIDEDNNSLHRQLIQIKKQIFDPFYL

NFPMFDRLPIPSRLLAFQNVENFRSSLVDKVQKQLIKTYKFEQANTSGSALVRAYNNG

ELTYKQLTDNIVILLVAGHENPQLFISNLIYLLGKYHDTWQVDIRNEILNNEENNLSE

LPLLNSFLYECLRYYPPLSVIINRKTTKRCMLGPGIVVPKDTYVGYHNYSTCHDSNFW

GHTSDIFDPTRWGKDIETINKAWKTTKNNCILNTFHGGKRACLGEKLVFVSTRIIIAE

FLSSFEWKLSPLWVEQMTHAGPLCPKDLALDIRVRDTSMQIQKLNKYL

 

>CYP56A6 Ashbya gossypii (= Eremothecium gossypii)

AE016819, NP_985947.1, 54% to CYP56A1

MTANIVLVLLGGLVLNYIRSIVLPPWGFPRNIPTIPFYVTFLTT

LFDIDQKELFELYIRKPMEKYGAVKIYFGNRWNILVSKPEFLAQMFKDEDTFAKSGNH

IKIPYSILALYTGDNVISSHGLAWKKFRGALTQGLQFFDPSPLSANAEKFIGFIERDM

KKNNGEVLMPALIQRLALANIAQIALGFDIGTLDPERPSKLQQQLEEVKKHIFHPLYM

NFSFLDRLPIPSRIRARQQVERFRSDLLQEVRRNLIINYKYEQTSYAASDLIRVYEHG

EITEKQLTDNIVILMVAGHENPQLLLTTCLYMLAKYPEWQARLRQQAVTLQDDLLNDS

VEFNQFLFETVRVLPPLGQIINRKTSHKCKLGPEIVLPKDTYVGYQVYGTGTSTQVWG

PDAAEFKPERWGSTNATVHETWRRSKNTGAMGAFHGGRRACLGEKMGLMETRVALAHM

LRKLEWTLSPNWKDRVTPAGPLCPFMLKLQFTRIQEPLAAELTA

 

>CYP56A7 Kluyveromyces lactis NRRL Y-1140

XM_452498, KLLA0C06743g

49% to CYP56A1, 53% to 56A6

MLSLVLGIISSGILLSFLCLVCFPIGLPWSLPTIPIYVSFLPIL

TDMDQLEIFERYIRHPMEKYGAVKIFFANRWNILTSKPEFLNIMFRDEDTFAKSGNQK

KIPYSLLAKYTGDNVISAHGKVWMKYRSCIQNQLNVFDSKPLIDNAQLFVKLLKKSIE

DNTEGTVLLPPLIQRLTLANIAKIALGFDIGTLKFDEHDNICRSRLHEKLNQVKQQIF

KPVFLSFPFLDLLPIKSRQRAKIDIIQFRDMLLDTVYKNLIHNYKFEQTNNAAAGLIR

EWKLQGITDEQLKDNLVIILVAGHENPQLLLSTLFYLLAKHQNWQYAIREELLSVDRN

EDILNSFKLTAFIYEALRMFPPLGQIINRRTTRKCQLGPGIIIDKDVYCGYNVYGTGT

ATSVWGETAKQFIPERWGENSNELANNWKKHKYDASMSAFHGGRRSCLGEKLALMEMK

YVLYYVLMNFHIELHPQWKEKMTPAGPICPKMLKVKLTILKDPQDQRSDSLYSSAGET

LSENI

 

>CYP56A8 Saccharomyces mikatae

90% to CYP56A2 Saccharomyces paradoxus Smik_4197 partial seq

MGPLSQRLALDNISQVALGFDFGALKQEKNAFHEHLICIKRQIFHPFFLTFPFLDLLPIPSRKKAFKDVASFRELLVKRV

QDELINNYKFEQTTFAASDLIRAHNNEIIDYNQLTDNIVIILVAGHENPQLLFSTSLYLLAKYSNKWQERLRREISGITD

PKVLADLPLLNAFLFEAVRLYPPLSTIINRCTTRTCKLGAEIIIPKNVYVGYNNFGTSHDPKNWGSTAYDFEPERWGSDI

ETIRKNWRTAKNRCTVTGFHGGRRACLGEKLALTEMRITLAEMLRQFRWELDPEWKEKLTPAGPLCPFNLKLKFKKNVAE

 

>CYP56A9 Saccharomyces kudriavzevii

AACI02001147.1, AACI02000561.1

85% to CYP56A3

MGIFKILGLILFLIISYIAFVVVVPPLNLPKNIPTIPFYVIFLPVIFPID

QTELYDLYIRESMEKYGAVKIFFGSRWNILVSRSEYLSQIFKDEDTFAKSGNQKKIPYSA

IAAYTGDNVISAHGKVWRDYRNAITKGLQHFDDAPISKNAKLFCNKIKERVQNGQVSVHM

GPLSQRLALDNISQVALGFDFATLKHEKNALHELLIHIKKQIFH

PFFLTFPFLDLLPIPS

(67 aa gap)

PQLLFYTSLYLLAKYSSKWQERLRQEVKNVTDPKALADLPLLNAFLFETVR

MYPPLNTIINRCTTKVCKLGMDIVIPKNVYVGYNNFGTSHDAKNWGPTANDFEPDRWGSD

IETIRKNWRLAKNRCVVTAFHGGRRACLGEKLALTEMRITLAEMLNQLKWSLDPEWEEKF

TPAGPLCPLNLMLRFKENVAK*

 

>CYP56A10 Saccharomyces kluyveri

AACE03000001.1 Sklu_Contig1634.3 Cyan region is an insertion

60% to CYP56A3   Saccharomyces bayanus w/o the insert

MITTIALSFLTLIVIFSLKIIVPPWNFPNNIPTIPFYVTFLTTFFDLDQEDIFRLYLAEPLEKYGAVKIYFGSRWNILVS

RPTYLSQMFKEEDTFAKSGNQKKIPYSVMAEYTGDNVISAHGKIWKLYRNSVTNGLQFFDHKPMVENARLFCSLLKEQLNKE

CGQNLNYNKLSDKSKKEPCSNKAISLPECDDTNISILSQ

SASLEMADYIQRLTLANISQVALGFDFGTLTEDKAPLHQ

QLKYVKKQVFKPLFLNFPFLDKLPLPSRQSAREEVSKFRKNLVDQVRENLMDNYLYEQTSFASSELIRNYNREKLNEKQL

TDNIVIIMVAGHENPQLLLTTLLYMLAKHHSTWQVRLREELRSCSMTEIHELPLLNAFFFECIRMYPPLGQIINRCTSKT

CVLGRDIVIPKGSYVGYNVYATGRSRTVWGNDSNEFRPERWGLSYNEVMDTWRHSKNSCAMSAFHGGRRACLGEKLALTE

LRITVAEMLTQFQWRLAPDWKDKMTPAGPLCPLNLKLKIEPLNQ

 

>CYP56A11 Kluyveromyces waltii

59% to CYP56A10 Saccharomyces kluyveri kwal_162-g20.1

similar insertion as in 56A10

MNSILWGLIGVIFFLILRIVVPPFNFPRNIPTIPFYVTFLATIWDLDQEDIFNLYLREPLEKYGAVKLYFGSRWNIVVSR

PEFLAQMFKDEDTFAKSGNHKKIPYGVIAAYTGENVISAHGAIWKTFRRAITPGLQLFDHTPLVKNADLFCSLISKRLTK

SGSSSIGGSTSSLDEKESWQFPTA

ASGTLLMSELIQRLSLANISEVALGLDIGTLTEESPELHLKLKNVKSHIFKSLYLN

FPLLDKLPIPSRQRARGYVEDFRRCLMKAVEMNLLNNYTFEQTSFSSSDLIRSYNREEISKKQLIDNIVIIMVAGHENPQ

LLLTTCFYMFGKNRKWQDLVREEVQGCGIENLNDLPLLNSFIFECIRMYPPLSQIINRCTTKKCVLGTDIVLPENVYLGY

NVYSTGRYKSAWGDNADCFIPERWGNNIEEITKEWRHRKNTCAMAAFHGGRRACLGEKLALMEVRIAMAAVLKNFEWTLA

ASWVDKVTPAGPLCPAGLEIEISELSKH

 

>CYP56A12 Kluyveromyces polysporus

Kpol_364p9

62% to CYP56A3   Saccharomyces bayanus

MEFFIKFIIFCFVIFGVFIFKVVSPPLNFPRNIPTIPFYVSFLPIFYSIDQTELFELYLRKPLERYGAVKYFFGSRWNIL

VSKRLFVKQMFKNEDVFAKSGNQKKIPYSLLALYTGDNVISAHGEIWKTYRSVVTNGLQHFDYEPLYKNSKLFCDLIEKS

LETEENSESTVTVGPLIQRLALANISQVVLGFDFGTLTEEKCALQDHLIRIKKQIFHPFFLTFPFFDMLPLPQRRKGFKD

VSAFRNELVSKVRNDLINNYKFEQTTFTSSDLIRAYNNKLINYQQLTDNIVVLLVAGHENPQLAITTILYLLGKYHDSWQ

IAIRNEIGKLSKHDDLLQLPILNSFIFEALRVYPPLSIIINRCTTKTCRLGNDIVIPKGAYVGYHNYSTTHNFESWGDDS

DEFNPNRWGKTIEAIFHNWKVAKMECTLPTFHGGKRACLGEKLALAELRLCIAEILKRFEWKLSKSWNNKMTPAGPLCPL

NLKLDFKKIESE

 

>CYP56B1 Aspergillus nidulans AN2706.1 43% to CYP56A1 547 clan

revised 7/18/07

MLVTLTVLFLGLVGILASFLTYLFTPPQFFPKGLPTIPFYYTLIPLLNQT

LKRAPTDQVTLYHRYLSTPLRTHGAVKLFFGGRWNILITKPSYIAEVLKN

EDLYAKSGNQKKIPHSVLAQYTGDNIISSHGENWKLYSSIFKPGLQRDYD

PSGIWRNASLLVQMISQDIKRKSPVDINPLMQRYALANLSEVLLGTTFDT

LQKPAASLHAFQLLIKPKIFDPIFLNFPVLDYLPLQTREEARKLVTRFTD

ELIETVRKGHTTCDHEKEHTRNLGCRLLYACESGLFTEMQLRHNMISAFL

AGHENPQLLLVSSLFLLAEHPEMQESLRAEISALNDLEPAYNALSALPLL

TSTIYEVLRLYPPISQLINRRTTAPTLLGGEIPIPAGTYVGYNAYATNRD

IGFWGPDANEFKPSRWGNTMEEINALFRRANAKGAFISFHGGRRTCLGQR

FALLEGRVTLAKLLMCVRWEIDPSWQRGMTPAGPLYARNLQLRFSNISGAGKAGAS

 

>CYP56B2 Uncinocarpus reesii

59% to CYP56B1   Aspergillus nidulans

UREG_00550.1

MLTAYSLFSTGLLAVAIITILLLFVAAYLFSPPGSFPRNIPTIPFYYALLPLVKDNDQEVLYRRYLEPLFEKHGAAKIFF

GGRWNILVQRPSYIAEVFKFEDIYAKSGNHVKIPHSVLAEYTGDNIISAHGENWKLYRSVLQPPLQQDQDPEPIWRNARL

LIDLLFEEQKSSAGGSVLLPQLLQRYTLANLAEGLLGTSFETLQRKDAALHEFQMQIKPLIFSPVFMNFPFLDHLNLPSR

KKARQLARQFASKLCAVVQQGHQHAHNDKSTSNVGCSVIGAFEEGILTEKQFRNNMVSVFLAGHENPQLLLVSMIFLLGE

HQAVQEKVRRELFSLGDDELAYPVLQSAPYLTSVIYETLRMYPPISQLINRCTTRPVLLGGKIPLPAGTYVGYNAYATNR

DQEFWGADADEFKPERWGQTAEDINTMFRKANAKGAFISFHGGRRVCLGQKFAMFEARITMAAVLRSVEWKIDPAWPRMM

TPVSNKIQSHDATDINSVGIDRLGHCIREICESDSSGSARNIPNGTMLIYPNSTGTDRQYHARSERAIPKSNRPAPAGRE

EALNLENAPCLDSSAMFIDHSSALATCASKCCVPVSLNTAAQPLP

 

>CYP56B3 Coccidioides immitis

80% to CYP56B2   Uncinocarpus reesii

CIMG_00523.2

MAGLLSSLSSVTLVAVAAITVILFLALVYFFIPPASFPRNIPAIPFYYALLPLVKDGDQEALYHTYLEPLFEQHGAAKIF

FGGRWNILVQRPSYIAEVFKYEDTYAKSGNQKKIPYSVLAEYTGDNIISSHGENWKLYRSILQPPLQQDQDPGPIWRNSR

LLISLLLEEQKTSPHGSVLLPQLLQRFTLANLSEGLLGTSFQTLQQKDAALHKFQMMLKPLIFDPVFLNFPFLDHFNFPS

RQRARRLARQFANELCQTVQRGHQHAHTDKSTSNVGCSVIGAFEQGILSEKQFRNNMVSVFLAGHENPQLLLVSMMFLLG

EHQEIQERVREEVLSLGEDELPYSVLQSAPFLTSVIYETLRMYPPISQLINRRTTKPVLLGGKIPLPAGTYIGYNAYATN

RDKQSWGADADEFKPERWGRTVDEINVTFRKANAKGAFISFHGGRRACLGQKFAMFEARIAMAEILRSLRWKIDPTWPRR

MTPVSGLNQNHDAMDEN

 

>CYP56C1 Aspergillus oryzae

AP007164.1c

third P450 of six on this accession

CDS join(1160825..1161472,1161531..1162415,1162505..1162558)

47% to CYP56B1 AN2706.1 Aspergillus nidulans

41% to CYP56A1

MVMHGLCIIIGKALAVGILFLSICIFSRIVPPRYPRNIPAVPFW

VTLLSLVRDIDQEDIYRRHIQKPLQTHGAIKIFFAGQWNLLIQRSSYLNEIFRNEDVY

QKSGNQKKIPHSVLAEFL (1?)

GDNVISSRGTTWRLYRDIITPG

LQGHFDAGLIAANAEELCSSLLAFQNTVGNCGVPVQDLLQQFTIANVSQVLLQANHKP

TKGDESSLHQLQLAVKREIFKPIFMNFPVLDRLGKLIPCRVRARNVVEQFSAALQYGV

RHGQGTPNASNLGARLMAARDGGVLTGKQFRDNLNVLFVAGQENPQLLLISMLYLLAKHP (0)

DVQSRLRREIDACSTLDPSNVAFSELPYVTCVIYESLRLLP

PISQLINRRTSQDVILGNQIYIPKGTYVGYNCYSTNRDPAVWGPTADEFRPERWGQSN

TEISQCYRQRRARAEFVSFHGGSRACLGEKFALLEARVALFVLVSRLSWSLDPEWPDR

KTPAGPLYPRALRLIFTERK

 

>CYP56C1 Aspergillus flavus

98% to CYP56C1 Aspergillus oryzae

AFL2G_06061

MTLLRITPAKSKRLRVLVGPMRSSVNPASILWHPRGHKRSVFFPKSEQPTSRKMVMHGLCIIIGKALAVGILFLSICIFS

RIVPPRFPRNIPAVPFWVTLLSLVRDIDQEDIYRRHIQKPLQTHGAIKIFFAGQWNLLIQRSSYLNEIFRNEDVYQKSGN

QKKIPHSVLAEFL (1)

GDNVISSRGTTWRLYRDIITPGLQGHFDAGLIAANAEELCSSLLAFQNT

VGNCGVPVQNLLQQFTIANVSQVLLQANHKPTKGDESSLHQLQLAVKREIFKPIFMNFPVLDRLGKLIPCRVRARNVVEQ

FSAALQYGVRHGQGTPNASNLGARLMAARDGGVLTGKQFRDNLNVLFVAGQENPQLLLISMLYLLAKHPDVQSRLRREID

ACSTLDPSNVAFSELPYLTCVIYESLRLLSPISQLINRRTSQDVILGNQIYIPKGTYVGYNCYSTNRDPAVWGPTADEFR

PERWGQSNTEISQCYRQRRARAEFVSFHGGSRACLGEKFALLEARVALFVLVSRLSWSLDPEWPDRKTPVGPLYPRALRL

IFTERK*

 

>CYP56C2 Uncinocarpus reesii

60% to CYP56C1   Aspergillus oryzae

UREG_04911.1

MFSGALIAMLSKVLLAGVVVLTLSLYWVLVPPRHPRNIPAVPFWVTLLPLFFDVDQENTYRRYIRKPLQQYGVIKIFFGG

QWNLVIQRPTYVAEMFKHEEVYQKSGNQKKIPHSVLAEFLGDNIISSRGDDWKLYRNIIKPGLQKTFETQPIVANARKLC

SLLKKSQQALGSQGILIQNHIQCYTTANISKCVLQAEIESLSTENDTLMKLQLAVKREIFKPIFMNFPLLDRFGSFIPSR

AHARELVRRFSSELESRLVRDHTEMPDSEASDNVGSRLIAARDRGELTQQQFRDNLNVTFVAGQENPQLLLISTMYLLGK

YPEVQCRLRSEIEGCGADEPSHLILRDMPYLTSVTYESLRLFPPISQLINRRVAKPVVLGGEIYIPQNTYVGYNCYATNR

DPTVWGKTADEFRPERWGSTTKEISMRYRVARARAEFTSFHGGSRACLGETFALLEMKLSLFILLKNLSWRLDPKWEDRK

TPVRNLPRPLF

 

>CYP56C3 Aspergillus clavatus

53% to CYP56C2

ACLA_064250

MDVFFSEWSKTATGWSLLLFLGALPCLFGLYLCFVAPPRHPQQIPSVPFWVTLLPFFFDVDQQETFQQYIAKPLQTHGAI

KMFFAGRWNVIVQRPAMLAEIFRNEHIYHKSGNQEKIPHSVLAEYL (1)

GSNIISARGDEW

RRYRAVIQPGLQTSFDSSAVFRHAQRLSAVLLGLQERNRRQGVPVQGVLQRYSSANLLRCVLGLSDLSMIKMDQDAPLHS

IQTSLKRYLFQPIFLNFPVLDWLGWAIPSRRQARNLIQDFSTELQMQIVQAHPPGSSTTSVGGRLATAWRDGTLTTQQFR

DNLNVLYVAGHENPQLLLTSALYLLGKFPKVQERLRAEIEQYGTIDEGDTPTVSWERLPYLSATILECARLFPPISQLIN

RRVATPTWLAGNLHLTPGMYVGYHSYATNRDPTTWGPDADEFRPERWGHTHEQIFAAYRRAKARAEFITFHGGPRACLGE

KFALLQMRVSLLVLVRSLSWTLDPEWTDQMMPAGPLHPRGLRLMFITHDKDAAT*

 

>CYP56D1 Clavispora lusitaniae ATCC 42720 = Candida lusitaniae

AAFT01000066.1, CLUG_02306.1

46% to CYP56A1

MLIDIIISNISVSFWCLCFFFLSWKIFRIIYPPFDFPKNIPTIPFYAAFVSSFLGWDQNK

VYAHYYRENLEKYGAAKIYFASRWNILVTKPEYLAQIFKQNEIFEKSGNQHKIPYSLLAE

YTGDNIISTGNKYWKLYRKAVTNSIQFPNLEPLQTNATVLIENLSAASDNGYFPIADILQ

AYTLNNVGDCVIGTNFHKSDNFDISTSLKKVKQEIFRPLFLNFPLLDLLPFPSRIRARKL

VRSFKRNYCNKILNELNEENKGRLGHNLATFWKQGDITEKQFQDNAMIAMIAGHENPQLL

LTSLIYVIAKYPEIQRQLRSEMNSSNYDVKEQIPLLKAVVYETIRLFPPLGLIVNRKTRK

NVILGNGIFIPKGTYVGYNNFGTQRDRNYWGDDADAFRPERWGNDGKKIEEKYAEAKSKC

TLPAFHGRSRACLGEKFALAEVRKAVIEILQNFEISLDPSWKEQLTPAGPICPLKLSVML

KKVCSEHEHNI*

 

>CYP56E1 Candida tropicalis MYA-3404

AAFN01000070.1

45% to CYP56A1

MIMFDIVKYTLIGLFSYLLYVILDIVLPPFNFPKNIPTIPFYVSFLGAYTNLDQRDI

YNLYLREKLEKYGAVKIYFASRWNILITRPEYLLEMFRNEDVYSKRGNHLKIPGSVMATY

TGDNIISAHGELWKLYREVIAKSIQFPDFEPITKNTKSLLEIIDGMIDSDKNHAIIPITD

LFQKYSLANVTESILGVNFKVLEGDQSIMHQKIKYVKLQIFKPFFLNFPYFDSFPIPSRL

QARKEVINFRNWYGQSIIDKHDPQLPNSAATKLVDGLMQEKLTEKQFLDNAIIVMIAGHE

NPLLLMLSLMFVAAKYPKVQEAIRSEIDPTKPYLHSVIYETLRMYPPLGLIINRYTTRPT

KLGNIVIPKGVYCGYNNFGTGRDRNVWGPDSDEFKPERWGRDNIEEINRNYANAKRSAEL

PAFHGRKRACLGEKYALYEVKELLTSILGHYKVTLDASWKEKITPAGPISPFGLKVKFEK

LIVA*

 

>CYP56E2 Candida albicans SC5314

AACQ01000054.1 chromosome R Ctg19-20053, whole genome

45% to CYP56A1 missing N-term runs off the end of the contig

whole seq on orf19_554

MFQLLKYIFIGGFTYLLYLILEIVFPPFNFPRNIPTIPFYVSFLGACTNLDQE

DIYKLYLREKLEKYGAVKMYFASRWNILITKP

151305  DFLLEMFKNEDVFAKSGNHVKIPNSVLATYTGDNIISAHGELWKLYRDVVAQSIQFPDLK  151126

151125  PISKNTQKLLRFLDNEMGSANQATISVTDILQKYSLANVCESVLGVNFNVLDDKQSLMHE  150946

150945  KIKYVKSQIFNPIFLNFPYFDNFPIPSRLKARREVIGFRKWYGQSLIEKYNLQLPN  150778

150777  SAATKLVDSLIKEKLTEKQFLDNAIILMIAGHENPLLLMLSLLYVVSKYPQVQELIRNE  150601

150600  TETTKPYLHSVIYETLRMYPPLGLIINRCTTRITKLGNIVIPKNVYCGYNN  150448

150447  FGTGRDRNVWGSDADIFKPERWGLEIDEINKKFTLAKRSAELPAFHGRKRACLGEKYALF  150268

150267  EVKQFLLAILGEYKVSLDPNWKEQLTPAGPISPLRLKLNFEKLTVS*  150127

 

>CYP56E3 Candida dublinensis

96% CYP56E2   Candida albicans (ortholog)

cdub_14-g334.1

MLQLLKYIFIGGFTYLLYLILEIVFPPFNFPRNIPTIPFYVSFLGACTSLDQEDIYKLYLREKLEKYGAVKMYFASRWNI

LITKPDFLLEMFKNEDVFAKSGNHVKIPNSVLATYTGDNIISAHGELWKLYRDVVAQSIQFPDLKPIFKNTHKLLRFINN

EMGSANQTTISVTDILQKYSLANVCESVLGVNFNVLDDKQSLMHEKIKYVKLQIFNPIFLNFPYFDNFPIPSRLKARREV

IGFRKWYGQSLVEKYNLQLPNSAATKLVDSLIKEKLTEKQFLDNAIILMIAGHENPLLLMLSLLYVVSKYPQVQELIRNE

TEPTKPYFHSVIYETLRMYPPLGLIINRCTTRITKLGNIVIPKNVYCGYNNFGTGRDRNVWGSDADTFKPERWGVEIDEI

NKKFTLAKRSAELPAFHGRKRACLGEKYALFEVKQFLLAILGDYKVSLDPTWKEQLTAAGPISPLQLKIKFEKLTVS

 

>CYP56E4 Candida parapsilosis

65% to CYP56E1   Candida tropicalis

CPAG_05515

MINSLKGILWFRFVHNGTMVMFEFIKVVAASLLGFGLYLLLEIVFPPLCFPRSIPTIPFYVSFLSSWTNLDQRDIYNTYL

REKLEKYGAVKIYFASRWNILVTRPEYLIQVFKHEDIYAKSGNHLKIPGSVLASYTGDNVISAHGPLWKLYREVVSSSIQ

FPDLSTIHQNTNKFLDLLEQDLSTKSSILVNDLLQRYTLANVGDSILGVDFKALDGKESIMHQKIKYIKQQIFKPFFMNF

PYIENFPIPSRIKAKREVEGFRNWFGQNLSSNYNPERGNSAATKLAQALCNEKLTQKQFLDNAIIIMVAGHENPLLLMLS

LLFVVAKYQDVQELVRSETNPEQPFLCSVIYETLRLFPPLGQIINRCTTSSVCLGDIRIPRGVYVGYNNMATGRDRSVWG

ADADQFKPSRWGTTISEINTNYSSSKRLAKLPAFHGRKRACLGEKFALHESKLLLTSVLEKYKVSLDENWQEKITSAGPV

GPLGMKLKFEKV

 

>CYP56E5  Lodderomyces elongisporus

runs of EE probably in an intron seq

59% to CYP56E2   Candida albicans

LELG_03266

MIVLFITILLVLSALIVFPPLNFPRNIPTIPFYVSFLGGMDQLEKFEKYLRTKLEKHGAVKIYFASRWNILVTKPEYLVH

IFKNEDVFAKSGNHIKIPGSVLATYTGDNIISAHGQLWRLYRDVMAQSIQFPNLDPIAENTNNLMKDLENALKFDSSVAI

TDFLQQYSLANVGDCIVGSNLRPMHQKIKYIKLQIFKPFYMNFPSVEMLPIPSRIRAKQEVENFRQWFGDKLMDADHGAA

VKLATAYNEGKLTQKQYLDNCIILMVAGHENPLLLMLSLLYVLAKNPYMQDKIRCNKE

IEEHGKEEGGRVVEEEGEEGEEEEGEEEEEEEEEEEEEEEEK

SPELYLSAVIYETLRMYPPLNQIINRCTTKDTMLGNIRIPKGTYVGYYNMGTGRDKTVWE

SPDTFWPERWGNTIEEVHANYTRAKRLGHLPAFHGRKRACLGEKFALYQCKAMVQAILGKYKISLDEDWEERITPAGPVC

PMNMKLRFSKI

 

>CYP56E6 Pichia stipitis

57% to CYP56E4   Candida parapsilosis

JGI model e_gwh1.2.1.1913.1

MISALIVGVLLIVKSIILPPSNFPKSIPTIPFYVSFLGAYTSMDQEEIYNYYMREKLEKYGAAKLYFASRWNIVIIEPEL

LLQLFKNENIYAKSGNQEKIPYSVLAQYTGENIISAHGEKWRLYRRVVASSVQFPNMEPIVENSKRFLDVLHQELINNSP

IEIIDLLQRYTLANIGDSVLGVKFNTMEEKDTEIHKRIKHVKRQIFQPLYMNFPFLDKLPIASRMEARKEVAQFRSYFSN

ILKTCQNFGRETGASRLRQALDNNTLTEKEFTDNGIILMVAGHENPLLLLLSLFYIVAKYPEVQHKIRQENGDNEAPYLN

SVICECLRMLPPLGQIINRRTTENVILGSNIRIPKGTYVGYNNFGTGRHRKIWGESADEFKPERWGKTPQEISRKYLEAK

IKANLPAFHGRKRACLGEKFALEEVRVLIQTLLQMYQLLLDPNWVEKITPAGPICPLGLKIKFKKIEF

 

>CYP57A1v1 Nectria haematococca L20976

MLVDTGLGLISELQAKLGWAVLLQIVPITIVAYNLLWFIYASFF

SSLRKIPGPFLARISRVWEMKKTATGNIHEIMMDLHRRHGAIVRIGPRRYDFDTMEAL

KIIYRIGNALPKADYYKPFGLPSFPNLFDEQNPARHSAIKKQVASLYTMTALLSYEEG

VDGQTAILKEQLQRFCDQKQVIDLPRFLQYYAFDVIGVITVGKSMGMMESNSDTNGAC

SALDGMWHYASMMAYIPNMHAWWLRLSSLLPIEVPIKGLTEYVERRIIQYRLKAAEFG

DDAALKGENNFLAKLLLMEKKGTVTPVETQQAVGLNIGAGSDTTANALSTILYYLYTN

PRTLHTLREELERYVKDGPISFQQSQSMPYLQAVIKEALRLHPGVGTQLTRVVPKGGL

VIEGQFFPEGTEVGVNGWALYHNKAIFGNDASIFRPERWLEANENINIGGSFAFGAGS

RSCIGKNISILEMSKAIPQIVRNFDIEINHGDMTWKNECWWFVKPEYKAMIKPRRCCL

SRDESLV

 

>CYP57A2 Nectria haematococca X73145

MLVDTGLGLISELRARLGWAALLQIVPVTVVAYNLLWFIYTSFF

SSLRKIPGPFLARISRVWEIKKAATGNIHEIVMDLHRCHGPIVRIGPNRYDFDTMEAL

KIIYRIGNALPKADYYIPFGLPSSPNLFDVQNPARHSAMKKQVASLYTMTALLSYEAG

VDGQTIILKEQLQRFCDQKQVIDLPQFLQYYAFDVIGVITVGKSMGMMETNSDTNGAC

GALDAMWHYSSMMAFIPHMHAWWLRLSSLLPIDVPIKGLTEYVEQRIIQYRLKAAEFG

DDDALKGENNFLAKLILMERQGTVTSTETQQAVGLNIGAGSDTTANALSSILYFLYTN

PRTLRRLREELDTHVKEDPIRFQQSQSMPYLQAVIKEALRLHPGVGTQLTRVVPKGGL

VIEGQFFPEGAEVGVNGWALYHNKAIFGNDASVFRPERWLETKGNLNIGGSFAFGAGS

RSCIGKNISILEMSKAIPQIVRNFDIEINHGDMTWKNECWWFVKPEYKAMIKPRAA

 

>CYP57A3 Nectria haematococca

fgenesh1_pg.scaffold_15000311

Necha1/scaffold_15:835216-837380

61% to fgenesh1_pg.scaffold_36000065  CYP57A1,

MLIDAIRLKMWELYSSRSLSDLNNLLLLGQ

ICYTIVWLFHAFFLSSLRKLPGPFLARLTRLWEVRKVTTGDIHDIMIDLHETH (1)

GPIVRIAPNRYDFNTPEAVKIIYRIGNAFHKSHFYDPFGSPQFKNLL

NELDNSRHAAMRRQLASLYTMSTLMSYESAVDGQTLILREKLLKFCDQGEVIDVPQFLQFYAFDVIGVIT (0)

VGESMGMMESNTDMHGACRDLDAIWRYAAVVGLLPSLHPWVVRISSFLRLRGVSDGLDSFVDAQIRQYKE

KQGSPDANTNEDTTFLSAMLKLERQGKATEAETRLCLSMNIGAGSDTTAISLSSIIYYVYTNPRTLRHLR

EELERATREGRLSDPIKFQEAQELPYLQAVIKEALRLHPGVGTQLTRIVPKGGVVIEGQYFPEG (0)

VEVGVNAYALYHNQDVFGKNASSFQPERWLESESEGIRIAGSFA (0)

FGAGPRSCIGKNISILEMSKAIPQIVQHFDIELEPRDKPWDNECWWFVKPQYKARIRRRTS*

 

>CYP57A4 Nectria haematococca

fgenesh1_pg.scaffold_30000136

Necha1/scaffold_30:357529-359333

63% to fgenesh1_pg.scaffold_15000311, 57% to 57A1 

This gene model seems correct DRN 2/5/06

MLVNSGFGITLAELHGSQALAILVKIFVAGYAFYIILWLVYILFLSNLRKVPGPFLAKLTRLWEVKKVIT

GNIHGIMIDLHKIH (1)

GPIVQIAPNRYDFNTPEAVKTIYRIGNAFTKSRYYEPFGDPSFHNLMNALDNKDHAALR

KQIASLYTMSALLSYEPLVDAQTTILKEKMEGFANRGDVVDLPLFLQFYAFDVVATIT (0)

IGKSMGMMESNTDMYDTCQVLDGMWHYIAVSGLIPGIHLRYIQLAKLLGLTPPTKALDFFIDTQIQHYTE

AMERKGGVGDDEDTFLARMLKLQEQGKATKKDTRHCVTINIGAGSGTTAIGLSSVIYHLYSNPHALNRLR

EELDEFVKAGELSDPVGFQQTQKMPYLQAVTKEALRLHPGVGTQLTRVVPKGGVVIEGQFFPEG (0)

AEVGVNGWALYYNQDVFGDDAAKFRPERWLQPGEDVRIAASFA (0)

FGAGPRSCLGKNVSILEMSKAIPQIVKNFDIEIQYESWKHESWFFVKPEFKAQIKLRVR*

 

>CYP57B1 Nectria haematococca

gw1.57.27.1

43% to fgenesh1_pg.scaffold_15000311, in  CYP57 family, model short at N-TERM

Necha1/scaffold_57:81298-82977

MALLRNLESSPIAQYVLSRLQHAGLKDLAAAFLLTSATLITVLYLVPLVYSLFFSPLRKIPGPLLTRVTH*

WEYRVVIKGDSNREYIRLHKKY (1)

GPVVRVGPNRYSVSQPKDIKTIYELGGKFTKSDYYKPLLNPNPEEQNIFPIQDNERHKERRRRISPLYTISSMVSY

EPAVDDITAVCMRKLYQFAEEGRLLDIPHWMQYYAFNIIGEIT (0)

FNKSFNMIENEGDTTGMIPGIREANDF

LAYLGVIPSLVPWVIGLSAVLGRKSNTSVLVSYTFAQINKNQEANAHSTTKDSKKYNTFLKKVLDMKSQG

RLKLPNVLDACGSNIGAGSDTTAVTLSSTLFFLYSNPNKLAKLRREIDTKAADGSISDPVTFQEAQNMTY

LQAVIKETLRLHPAVGTILPRVVPRGGIELSGHYFPEG (0)

TEVGVNAWVLHYSKDIYGPDPEVFRPERWIGDEKTSIMDSMMFA (0)

FGAGSRTYIGRNISLLELTKVVPQIVRKFDLEFELPGKPMDTTCAWFVYPHYNGRFKIRETKA*

 

>CYP57B2 Nectria haematococca

complete joined model 45% to 57A2, 83% to gw1.57.27.1

MALLQDLQSSLIAQRILSSLQHVSPKDVAIGFLLTAAVLYIFFYLIPLIYSLFFSPLRKIPGPLLARVTR

WWEYRLVSKGDSNLEYIRLHQKY (1)

GPVVRVGPNRYSISQPKDVKTIYELGGKFTKADYYKPLLNPDPEKQN

IFVVLDNDRHKEKRRKISPLYTMSSMVSYEPAVDEMTTVCIRKLYQFAKEGRLIDIPHWM QYYAFDVIGEIT  (0)

FNKSFGMMENEGDTTGMIPGIRAANDFLAYLGIIPNLVPWVLGLSAVLGRKSNTSVLVSYAFAQIDRNREANVHST

TKDTKKYDTFLKKLLDMEAQGRLKLPNVLDACGSNIGAGSDTTAVTLSSTLYFLFRNPDKLDKLRQEIDT

KTAEGSISDPVTFREAQDMSYLQAVIKETLRLHPAVGTMLPRVVPRGGMELSGYYFPEG (0)

TEVGANAWVLHYSKDIYGPDPEVFRPERWIGKEKTSIMDSMMFA (0)

FGAGSRTCIGRNISLLELTKVVPQIVRKFDLELEHPDKPMDTTCAWFVYPHYNGRFKVRGAKASD*

 

>CYP57B3   Aspergillus oryzae

          GenEMBL BAE55311.1

          56% to 57B2, 55% to 57B1

MIGTVLDTVLGNPQGAVGGLFVGSFVIFWVVPFLYNLFFSPLRNVPGPFWARFTILWEFSQLMKGRSHEE

YIKLHKKYGPVVRVSPKRYSVIDPQDVKKIYGFGADFPKSEFYDSLGDPKNIFTVRDNEDHKDRRRKVAS

LYTMSSMVAYEDAVDRMTTLCIKKMTDLAASRKLISIPKFMQFYAFDVIGEITFDQNFGMMENMGDTQGI

IKEIHAMNNTIGIMGLLPELQSTWRAAQKVLFSGTAMGKLTEYIWSQYWKHRNTNSGAKQKSQYDTFLRK

VLELEAAHKVGRMNVLDSCGSNIGAGSDTTGITLSAALWYIYRNPDKLAKLRHEIDTMTAEGRISDPVTY

KQAQEMPYLNAVIKETLRVHPGVGTILARVVPKGGITLTGGYYVPEGTHIGTNAWPLHYSETVYGPDADQ

YRPERWLEDKPQPDYRDSMMFAFGSGSRTCIGRNISLLEITKVLPQIVRKFDLKFEEKKDPWDAW

CAWFVYPKYKCWIEPRKPVENVA

 

>CYP57B3   Aspergillus flavus

100% to CYP57B3   Aspergillus oryzae

AFL2G_00227 revised

MIGTVLDTVLGNPQGAVGGLFVGSFVIFWVVP FLYNLFFSPLRNVPGPFWARFTILWEFSQLMKGRSHEEYIKLHKKYGP

VVRVSPKRYSVIDPQDVKKIYGFGADFPKSEFYDSLGDPKNIFTVRDNEDHKDRRRKVASLYTMSSMVAYEDAVDRMTTL

CIKKMTDLAASRKLISIPKFMQFYAFDVIGEITFDQNFGMMENMGDTQGIIKEIHAMNNTIGIMGLLPELQSTWRAAQKV

LFSGTAMGKLTEYIWSQYWKHRNTNSGAKQKSQYDTFLRKVLELEAAHKVGRMNVLDSCGSNIGAGSDTTGITLSAALWY

IYRNPDKLAKLRHEIDTMTAEGRISDPVTYKQAQEMPYLNAVIKETLRVHPGVGTILARVVPKGGITLTGGYYVPEGTHI

GTNAWPLHYSETVYGPDADQYRPERWLEDKPQPDYRDSMMFAFGSGSRTCIGRNISLLEITKVLPQIVRKFDLKFEEKKD

PWDAWCAWFVYPKYKCWIEPRKPVENVA

 

>CYP57B4 Fusarium oxysporum

81% to CYP57B2

FOXG_01386

MAFLRDAESNPTVQAIFSRLKHVDATAVAAGLPLNTFRSCLFILLHRITL

(sequence gap)

KLNQFAKEGRLVDIPHW MQYYAFDVIGAITFNKSFNMMENEGDTTGMIKGIREANDFLAFWGIVPNLVPWLIGIATALGMTSNTSTLVSYALKQIDN

TRKENAKSTVKGTTKYDTFLKKLLEGESEGRLKTPNLLDACGSNIGAGSDTTAVTLSSALYYLFQNPDKLKKLREEIDQK

AAHGGLSDPVTFQEAQDMPYLQAVIKETLRIHPAVGTILPRVVPRDGMELSGIYFPEGTEVGVNAWVLHYDKGIYGPDPE

VFRPERWLGDEKTSIMDSMMFAFGAGARTCIGRNISLLELTKMVPQIVRKFDLVIEDADKPLDTFCAWFVYPHYNGRFKV

RE*

 

>CYP57C1 Mycosphaerella fijiensis

51% to CYP57B3 Aspergillus oryzae

fgenesh1_pg.C_scaffold_2000556 revised N-term

MPSFEIQHSVLANLSAKQILALTSILLGITIAYQFVYALLLSPVKDV

PGPFLARFTRLWELRQMLRGEFHETIVKLHKQHGPIVRISPNLYSLNSPADQKKIYNLRADLSKTPYY

DAGGHPQRPNLFSTQDASLHADRKKPVANLYSMSSMVHYEDAVDRMIKICLRKFAGFAEKRKKVFVPKFMQAYAFDVIGE

ITINQNFGMMETEADVDGMMELVHASIRYLGTVGIFPEFHSWIFRIVSFLRLRHAGGLIEKIASDTIRKFRHPDRMIEKE

DPNSDPFISKLLRLEAAGKADSSHLMDAIASNIAAGSDTTAITLSAALYYLYRTPRALAALRKEIDEMQKAGKISDPVSF

KEAQDMPYLQAVILETLRLHPAVGYVLPRKVPAGGMELAGRHLPGGTCVGVSAWALHRNPEAYGDDASEFRPERFLVSED

SSSKNRTASLASSFAFGGGTRSCIGKNISLLEMTKAVPQIVRKFDLVFESDEPWELFSTWFVWQKYHCYIEPRKG

 

>CYP58A1 Fusarium sporotrichioides U22462, ALSO U10456 mini chromosome

ESTs BI191705 BI191704 BI191231 BI200306 BI201084

Formerly CYP579A1

MVDQDWIKALVNIPISHAVGVVAASTVIYFLSSCFYNLYLHPLR

KIPGPKLAAIGPYLEFYHEVIRDGQYLWEIAKMHDKYGPIVRVNDKEVHIRDPSYYST

IYTAGARKTNKDPATVGAFDVPTATAATVDHDHHRARRGYLNPYFSKRSITNLEPFIH

ERVTKLLSRFQEHLDNDQVLSLDGAFCALTADVITSRFYGKHYNYLDLPDFHFVVRDG

FLGLTKVYHLARFIPVLVTVLKRLPYSCLRLIAPSVSDLLQMRNEIHERGGDEFLSSK

TSEAKSSILFGALADTHIPPVERTVERMLDEGTVILFAGTETTSRTLAITFFYLLTHP

ECLRKLREELNSLPKVEGDRFPLATLENLPYLNGVVHEGFRLACGPISRSGRVATQEN

LKYKEHVIPAGTPVSQSTYFMHTDPKIFPEPEKFKPERWIEAAEKKIPLKKYITNFSQ

GSRQCIGYTMAFAEMYLAMSRIARAYDVELYDTTKADIDMTHARIVAYPKAIPGKTEH

VGEIRVKVLKAL

 

>CYP58A2 Myrothecium roridum AF009417 65% identical to 58A1 Fusarium sporotrichioides

MAVLNFETVSNAIPLGAVAGGIAGLYFLYLAWNCFYNIYLHPLS

HIPGDKLAVMGPYLEFYHEVIRGGQYLWEVEKMHERYGPIVRVNAREVHVKDSSFYHT

IYTAGSRKTNKDPSAVGAFDVPSSTAATIDHDTHRARRGYLNPYFSKRSLASLEPTIN

ERIGKLAERMNGHMNGKEILTLDGIFSALTADIICSRFYGKNFDYLSVPDYHFVVRDG

FQGLTKLYHLARFLPTMVNGLKVLPEWFVRMFLPPLADLMVMRQEIHTNGAEMFTNSH

TADSKASALVGALADTNIPVHERTISRLLDEGTVFLFAGTETTSRTMAITMFYLLSNP

DCLRKMREELDTLPASEGFAHSLQTLEALPYLSGVVNEGLRLAFGPITRSARVPMNVD

LQYQDYTIPAGTPLSMSTYFVHTDEELYPDPMAFKPERWIKAAEDGVPLKKFLTNFSQ

GSRQCIGINMSFAEMYLTLSRVAREFDFELYDTTMADLDLTYARIVGYPKAIPGKTEG

TGEIRVKVLNRHSPNFEKQELISTA

 

>CYP58A3 Fusarium graminearum old name = CYP579A2 FG03535.1 AACM01000159 FGcontig1.159_scaffold2

MIDQDWIKSLLNIPVSHVAGIFAASTVIYFLSSCFYNLYLHPLRKIPGPK

LAAIGPYLEFYHEVIRDGQYLWEISKMHDKYGPIVRVNAREVHIRDSSYY

TTIYTAGSRKTNKDPATVGAFDVPSATAATVDHDHHRSRRGYLNPYFSKR

TITNLEPFIHERVTKLLTRFQQHLDDDQVLSLDGAFCALTADVITNRFYG

KHNDYLSLPDFHFVVRDGFLGLTKIYHLARFLPGLVTILKRLPYSCIRMI

APSVCDLLQMRDEIQDRGGEEFLSNKSHEAKSSILFGALADSHIPSHERT

VERMLDEGTVILFAGTETTSRTLAITVLYLLTHPECLKKLREELNSLPPV

KDGQYSLATLENLPYLNGVIHEGFRLAFGPISRSGRVATQENLKYKEHVI

PKGTPISQSTYFMHTDPKNFPEPEKFKPERWIEAQQKGIPLKKYITNFSQ

GSRQCIGYTMAFAEMYLALSRIARAYDIELYDTTKADIDMTHARIVGYPK

AIPGKKEHLGEVRVKVLKAL*

 

>CYP58B1 Aspergillus parasiticus

AY371490.1 aflatoxin pathway gene

93% to CYP58B2P Aspergillus flavus NRRL3357

MASNTVYTSLIGLLVALTVRSIYRVYFHPLRKIPG

PKIAAITHLYQHYYDAVKGGKYIWKLDELHRKY (1)

GPIVRFNPNEVHIQDSHYYHHIYAGGAKKQDKDPGFPAVPLFPGVTVTTIKH

NHHRLRRGIIKSFFSKQYVTGLEHVIQSKVNLLASRFTEAYRHGTVLDLKYVFAALTSDL

TTHYVYGTNLNHLAEPDFKNDFLAGMDSVGPWIPVLLVFGRLLKLARYLPACLVPAGEFL

HLWTLSERRVGEILDSQDNGTMGDQKTLLQAMATADVSEEEKTATRLQMETLNIIAGGTE

TTARALAVGVFHLAHKPSLLLQLRDELRTVMPFPDSLASCTQLEQLPYL (0)

AGVVNESLRLSFGFIIRSARVYPNDPLVYEDLVIPPG (0)

TPISQSAYFVCMDPSIFPQPEDFNPDRWVQAAREGNNLHRYLIVFSKGSRHCLGIN (2)

FALAEIYLAIATIARRFDLVPYQTTVEQLQMKRDLGFAAPEKGPFTVRAKVTGLAD*

 

>CYP58B2P Aspergillus oryzae

Supercontig 10: 19609-20001 +

95% to CYP58B2P Aspergillus flavus

even pseudogenes are well conserved

TVTTIKHNHHRLRRGIIKSFFLKKYVTGLEHVVQNEVNLLASRFTEAYHHGTVLDLKY

VFAALTSDLTTHYVYGTNLNHLAEPDF &

KNDFLAGMDSVDPWIPVLLVFGRLLKLAGYFPACLVSAGEFLHLWT &

LSERRVGEILDSQDNGTMGDQKTLLQAMATADIPEEEKTATRLQVETLNIIAGGTET

TARALAVGVFHLAHKPSLLLQLRDELRTVMPFPDSLASCTQLEQLPYSYL (0)

AGVVNESLRLAFGFIIRSARVYPNDPLVYEDLVIQFP (?)

TPISQSAYFVCMDPSIFPQPDDFNPDRWVQAARDGNKLHRYLIVFNKGSR

HCLGIN (2)

FALAEIYLAIATVARRFDLVPYQTTVEQLQM

KRDLGFTAPEKGPFTVRAKVTGLAD*

 

>CYP58B2P Aspergillus flavus NRRL3357 pseudogene

AAIH01000612.1

missing first 29 aa, two frameshifts and one stop codon, bad boundary at 1273

AFL2G_07229

  76  LRKIAGPKIAPITHLY*HYYDAVKGGKYIWKLDELHRKY  (1) 192

 255  GPIVRFNPNEVHIQDSHYYHHIYAGGAKKQDKDPEFPAVPLFPGV TVTTIKHNHHRLRRG  434

 435  IIKSFFSKKYVTGLEYVIQSEVNLLASRFTEAYHHGTVLDLKYVFAALTSDLTTHYVYGTNLKX  623

 626  LAEPDFKNDFLAGMDSVDPWIPVLLVFGRLLKLAGYLPACLVSAGEFLHLWT  781

 783  LSERRVGEILDPQDNGTMGDQKTLLQAMATADVPEEEKTATRLQMETLNIIAGGTETTARALAV  974

 975  GVFHLAHKPSLLLQLRDELRTVMPFPDSLASCTQLEQLPYSYL (0)  1103

1163  AGVVNESLRLAFGFIIRPARVYPNDPLVNEDLVIPFP (?)  1273

1362  TPISQSAYFVCMDPSIFSQPDDFNPDRWVQAARDGNKLHRYLIVLSKGSRHCLGIN (2)  1529

1599  FALAEIYLAIATVARRFDLVPYQTTVEQLQMKRDLGFTAPEKGPFTVRAKVTGLAD*  1769

 

>CYP58B3 Aspergillus nomius isolate AN13137

AY510454.1

8295-10054

92% to CYP58B1 Aspergillus parasiticus

gene CypA of aflatoxin biosynthesis

gene model revised

MVSKTVSTALIGLLVALILRSVYRVFFHPLRKIPGPKIAAITHL

YQHYYDAVQGGKYIWKLDELHRKYGPVIRFNPNEVHIQDSHYYHQIYAGGAKKQDKDP

GFPAVPLFPEVTVTTIKHHHHRLRRGIIKSFFSKQYVMGLEHVIQSKVNLLASRLTEA

YRHDTVLDLKYVFAALTSDLTTHYVYGTNLSHLTEPDFKNDFLAGMDSVGRWIPVLLV

FGRLLKLARYLPACLVPAGEFLHLWTLSERRVGEILGSKNNGTMGDRKTLLQAMATAD

VPEQEKTATRLQMETLNIIAGGTETTARALAVGVFHLANKPSLLLQLRDELRTVMPFP

DSTASWTQLEQLPFL (0)

AGVVNESLRLSFGFIIRSARVYPNDPLVYEDLVIPPG (0)

TPISQSAYFVCMDPSIFPHPEDFNPDRWVQA

ACEGHNLHRYMIVFSKGSRHCLGINFALAEIYLAIATVARRFDLVPYQTTVEQLQMKR

DLGFAAPEKGPFTVQAKVTGLAD*

 

>CYP58C1 Aspergillus nidulans revised

MTVAQWMRWEVPLLLIISFYLTRTVYRLFFHPLTRFPGPKITAATGLYEF

YHDVVRGGKFLWEIEQMHQVY

GPIVRITPWEIH

VKDPHFYDEIYTSARKCNKDPNFVGMFGSPTSMIATVDHGHHRFRRGILS

SFFSKRSIRDISPLIRRKVQRLMERLAEFYQTDTEVDLSAAFAALTADII

STYSYGESFDFLEDGSFHSEVRDAIMETEKLDHISRLFPVVLTVIRHVPV

WAFAIVKPATAVIAGIQKRVAEKSAKALKMTGSSKEEWTMFDALTDPKLP

ACERTMSRIHDEGMILLSGGTEPTANALTVAAFHMINQKDILTTIREEVR

ASGRDVKKASLAELEQLPYLTAVVNEALRLSHGLSIRSPRISPTEALTYK

SYVIPAGTPVGMSNYFVHMDPSIFPHPASFNPNRWIEASKRGEKLDRFIT

AFGKGSRQCIGINLGYAELYFALASFATFFDYQLHDTTEENVRVARDRGV

PFPEAGHLTCLWRMWPSEGMILKAQISPEFRQCLLIVATRKAYARNGSML*

 

>CYP58D1 Aspergillus nidulans AACD01000172.1 Aspergillus nidulans FGSC A4 revised

MNRILAVTVSALASASERLTVSIPVLLILGLVVRTIYRLYFDPLHHIPGPKLAAI

THLYEFYHDVIRGGLFIWEIEKMHREY (1)

GPIVRINPREVHIKDSQFYEEIYAPASGGRRDKDRQAVEIFSSPTA

MVATVDHDTHRIRRKLLTFFFSRRSIERLEPVVHESLSKFLDSLVAAYKE

DTVVDLIDRLQGLTGDVITQYAYGNSYGLQEPQNIGRGITKVVQEGTTQI

HLHRFFPFFERLLRVVPKWFMAQMLPARAAIYDLLNGVREQSIEALKQRD

MSAPPPKKTTMFHALTAPEVPPEERTLQRLQDEGLVLFAAGTETTATILG

VALFHILNNKEVHRKLRNELDQELPTPQAGTTWRQLEKLPYLNATIHEAL

RCSGLTMRQQRIAPTEVVKYKNYVIPPGTPVSMISHFVNMDPDIFDDPQT

FKPERWILAAEKKQNLSRFLVTFGKGNRNCIGMNLAYAELYNTLAAVVRR

FDLELYQTTEENVRFVRDMLLPRSTNGPWKVRVKVIGIREE*

 

>CYP58D2 Aspergillus oryzae RIB40

AP007157.1b genomic DNA, SC023

73% to 58D1

complement(join(191351..191525,191581..191747,

191803..191910,191973..192829,192878..193118))

MGVISVVTFLASAKEHFIISILLLFPIALILRTIYRLYIDPLHH

IPGPKLAAISHLYEFYHDVVRGGLFIWEIEK MHQEY (1)

GPIVRINPREVHIKDPYFYDELYAPAHGWRDKDAKSVEIFSSPNA

LVSTVDHHTHRMRRKLLTSFFSRRSIERIEPVIHE

SLSKFLDSLITAYEEDSVVELIDRLQALTGHVITQYAYGEDYGLHEPQNIGKGIVKVV

QEGTEQIHLHRFFPLIQRFLRLIPSFFMTQLFPARAAMYDLLHGVRKKSIEVLQQKDV

CTPTERTTMFHALTAPEVPPEERTLQRLEDEGLVLFAAGTETTATTLGVAIFHILSDP

MVLTKLRKELEQVMPTPEGLATWRELEKLPYL NGVIHEALRFSGLAMRQQ

RVSPTEVI

KYKDYAIPPGTPVSMLQYFLHTDPALFSDPEKFYPERWMLAAERKESLSRFLVTFGKG

TRSCIGMNLAYAELHTALAAIVRRFDLELYETTAEDIRFVRDKLLPRAKNGPWRVRVK

VVGIRKD

 

>CYP58D2 Aspergillus flavus

99% to CYP58D2 Aspergillus oryzae

AFL2G_03972

MGVISVVTFLASAKEHFIISILLLFPIALILRTIYRLYIDPLHH

IPGPKLAAISHLYEFYHDVVRGGLFIWEIEKMHQEY (1)

GPIVRINPREVHIKDPYFYDELYAPAHGWRDKDAKSVEIFSSPTALVSTVDHHTHRMRRKLLTSFFSRRSIERIE

PVIHESISRFLDSLITAYEEDSVVELIDRLQALTGHVITQYAYGEDYGLHEPQNIGKGIVKVVQEGTEQIHLHRFFPLIQ

RFLRLIPSFFMTQLFPARAAMYDLLHGVRKKSIEVLKQKDVCTPTERTTMFHALTAPEVPPEERTLQRLEDEGLVLFAAG

TETTATTLGVAIFHILSDPMVLTKLRKELEQVMPTPEGLATWRELEKLPYL

NGVIHEALRFSGLAMRQQ

RVSPTEVIKYKDYAIPPGTPVSMLQYFLH

TDPALFSDPEKFYPERWMLAAERKESLSRFLVTFGKGTRSCIGMNLAYAELHTALAAIVRRFDLELYETTAEDIRFVRDK

LLPRAKNGPWRVRVKVVGIRKD

 

>CYP58D3  Aspergillus fumigatus Af293

         GenEMBL XP_753152.1 also AAHF01000003.1

         trichodiene oxygenase predicted

         71% to 58D1

         N-term and C-term parts of broken gene

MRRKLLNNFFSRRSIENIEPVIHNSLSRFLDGLTTAYKEGSVVELIDRLQGLTGDVITEYAYGENYGLQD

PENYGKGIVKVVQEGTDQIHFFRFFPLVSQLLRIIPSWFMGQVFPARAAIYQLLDGVRKQSILALQQKNV

STAPQRMTIFQALTAPEVPPEERNLQRLQDEGLVLFAAGTETTATTLAVALFYN

YILNDKRVLSKLRRELEQI

MPTPQHPTTWRELEKLPYLTAVIREALRFSGIVMRQQRISPTEALKYKDIVVPPGTPVSMIARFVHMDPT

IFPDPTRFHPERWMLAAGREEGLSKFLVTFSRGNRGCIGMNLAYAELYNTLAAVVRRFDLELNQTTPENI

RFVRDKLLPRSKDGSWTVRVKVVGIREQ

 

>CYP58E1 Nectria haematococca

fgenesh1_pg.scaffold_14000019

Necha1/scaffold_14:58063-59829

46% to 58A2 also 48% to 579A2, check 579A2 vs 58A2 = 65%

Note 58A2 and 579A2 are same family and same subfamily,

already revised names 579A2 = 58A3, not yet changed on blast server

This gene model seems correct DRN 2/5/06

Note this gene is near scaffold_14000017

MTENVFWIDKPAIAAAGAAGLFWISYLVCEVIYNLFFHPLSKFPGPKIAAIGTYYEFYHDVIRDGTYLWR

IEEMHRKY (1)

GPVVRINADELHIHDPDFYSKVYAGSKRVNKYGPAVAAFTVPRATLATIDHDVHRLRRSILN

PYFSKRAVTMLEPLINDKVDRLCGRLEEWIGQGTPVDLDAAMAALTADIVSIYFYGKDFDYLGGKDFKFV

VRDAILGLIRFYHFSRFVPAVANFINSLPIPIVRMIQPGAAALLESQQEILVEIQQSLNDKDNVKSKSVI

VGALGDPDIPAEEKTLDRLVDEGVTVIFAGTETTARSISVAIFHLLRDKSLLQKLRDELSTVEKGPDGQW

AYNQLEALPYL (0)

TGCVQEGLRLAHGPVIRLPRVSPNEALQYGDWLIPAG (0)

TPVSESTLLVHLDPSIFPNPNTFDPDRWVRAAAEGVHLSKYICSFTKGTRQCLGIN (2)

LAYAELYIAIAKIATSFEMELYNTSLEDVQVHHIRLTGYPKRGTGEIKATITSKA*

 

>CYP58E2  Aspergillus oryzae

         GenEMBL BAE59845.1

         61% TO 58E1, 47% TO 58A2, 41% to 58C1, 46% TO 58A3

MLDTISESRPNGLATLGVVVATFATYLALKALYNLYLHPLRKFPGPKLAAIGPYYEFYYDVMKDGMFLWE

MERMHQVYGPIVRVNANEIHIRDPHYYSTVYAGNHRSTDKYHDAVAAFSVPQASLATIHHKVHRLRRSIL

NPYFSKAAVTRLESAINERIERMCSRLEETMHYGQVVDLDAGFAALTADIVTTYFYGQNFDYLGNEGFKF

QVRDAILGLIQFYHFTRFFPWIANTIKKLPIPIMRLIHPGAAYLVSSQEEIKDSIRASLDKGNKADAKSV

IVQALEDPTIPPQERTLDRLGDEGTTIIFAGTETTARALSVGMFHILNNKTILKKLREELDTLPGVSSGV

YSHVQLECLPYLTGAVQESLRLSHGPAIRLPRVANDKALKYGDYIIPPGTPVSLCTVLVHQDPCIFPDPH

RFDPERWVKASKEGVNLDKFIAAFTKGTRQCLGINLAYAEIYLTFAKLIRTFNMEIYDTTVDDLTVHHIR

LTGAPKQGTGEVKVKVTEKIL

 

>CYP58E2  Aspergillus flavus

100% to CYP58E2  Aspergillus oryzae

AFL2G_06877 revised

MLDTISESRPNGLATLGVVVATFATYLALKALYNLYLHPLRKFPGPKLAAIGPYYEFYYDVMKDGMFLWEMERMHQVYGP

IVRVNANEIHIRDPHYYSTVYAGNHRSTDKYHDAVAAFSVPQASLATIHHKVHRLRRSILNPYFSKAAVTRLESAINERI

ERMCSRLEETMHYGQVVDLDAGFAALTADIVTTYFYGQNFDYLGNEGFKFQVRDAILGLIQFYHFTRFFPWIANTIKKLP

IPIMRLIHPGAAYLVSSQEEIKDSIRASLDKGNKADAKSVIVQALEDPTIPPQERTLDRLGDEGTTIIFAGTETTAR

ALSVGMFHILNNKTILK

KLREELDTLPGVSSGVYSHVQLECLPYL (0)

TGAVQESLRLSHGPAIRLPRVANDKALKYGDYIIPPG (0)

TPVSLCTVLVHQDPCIFPDPHRFDPERWVKASKEGVNLDKFIAAF

TKGTRQCLGINLAYAEIYLTFAKLIRTFNMEIYDTTVDDLTVHHIRLTGAPKQGTGEVKVKVTEKIL*

 

>CYP58E3 Neosartorya fischeri

53% to CYP58E1

NFIA_042080

Note: this seq does not have an ortholog in A. fumigatus

MLFQDLSFPAVIGAAFGVFAISVVAGCIYDLFFHPLRNFPGPKRAAIWSFYEFYYDVIRDGTYLWEIEKMHHKYGPIVRI

NSRSLHIHDPEYFSTIYAGSGRKVNKELSAVSGYTFPHSTISTLDHDLHRKRRAIVNPYFSKRAIADIEPVIHERLDALI

SRLAEAKGSTVDLTCAFSAFTADVVTYHFYGSHANYIGSKDFKYGLKDALTVLLNLYNLTRFLPVPATTLKNLPLPILGL

INPNFPE VVSAREANKKMVLNYLNKPDEEKNAMKDARSKSVIVSALTDPNVPDAEKTLDRLLDEGETIIFAGIDTTARTL

AVALFHLLNNKDVLMKLRKELQAIAKSDGQQWTTTELEAVPYMRGVVQEAIRLAYGLVVRIPRISPHEALQYNGFVIPPG

TPVSQSTYLVNNDASIFPNPQAFDPERWVKAAQDGVSLDKYMVSFSKGSRGCLGINLAYAKLYLGIARVATSLDMELFET

TEKDIAVYHTRGFAFPKEGDGAVKAQVTGLCK*

 

>CYP58E3 Aspergillus flavus NRRL3357

AAIH02002155.1

Identical to CYP58E3 Neosartorya fischeri (partial seq, upstream part in a seq gap)

VVSAREANKKMVLNYLNKPDEEKNAMKDARSKSVIVSALTDPNVPDAEKTLDRLLDEGETIIFAGIDTTARTL

AVALFHLLNNKDVLMKLRKELQAIAKSDGQQWTTTELEAVPYM ()

RGVVQEAIRLAYGLVVRIPRISPHEALQYNGFVIPPG

TPVSQSTYLVNNDASIFPNPQAFDPERWVKAAQDGVSLDKYMVSFSKGSRGCLGI (2?)

NLAYAKLYLGIARVATSLDMELFETTEKDIAVYHTRGFAFPKEGDGAVKAQVTGLCK*

 

>CYP58E3 Aspergillus terreus NIH2624

AAJN01000124.1

93% to CYP58E3 = ortholog

ATEG_04417.1

36715  MLFQDLSFPAAIGAVFGAVAISVAARCIYDLFFHPLRNFPGPKRAAIWSFYEFYYDVIRD  36894

36895  GTYLWEIEKMHQKY (1)

36993  GPIVRINSRSLHIHDPEYFNTIYAGSGRKVNKELSAVSGYTFPHSTISTLDHDLHRKRRA  37172

37173  IVSPYFSKRAIAEIEPVIHERLNVLISRLAEAKGSIVDLTSAFSAYTADVVTYHFYGTHA  37352

37353  NYIGSKDFKYGLKDALTVLLNLYNLTRFLPVPANTLKNLPLPILGLINPNFPLVVSAREA  37532

37533  NKKMVLGYLNKPDEDKKAMKDARSKSVIVSALTDPNVPDAEKTLDRLLDEGETIIFAGID  37712

37713  TTARTLGVALFHLLNNKDVLMKLRKELQAVAKPDGQQWTTTELEAVPYM  37859 ()

37910  RGVVQEAIRLAYGLVVRIPRISPHEALRYNGFVIPPG (0) 38020

38074  TPVSQSTYLVNNDPSVFPNPQVFDPERWVKAAQDGVSLDKYMVSFSKGSRGCLGI (2?) 38238

38301  NLAYAKLYLGIARVATSLDMELFETTAKAISVYHTRGFAFPKEGDGAVKARVMGLCK*  38474

 

>CYP58F1  Aspergillus oryzae

         GenEMBL BAE64028.1

         46% TO 58E2, 40% to 58C1, 45% to 58D1, 44% to 58B1

         46% to 58A3

MALLVILGIGFVTYLVFLSIYRLYFHPLSHIPGPKLAAITHGYEFYHNIIRGGLFIWELERLHEVYGPII

RINPREVHIKDPDYYDDIYASSARKREKDPVLVAQFGLEGSGFSAIDQETHHQRRAPVAKFFSKRAIENQ

EHLIRDSLNKLVGHFQEACRAHTVVSLDAAFAALTSDVIHQYAYGFNPNNLDKEGFNAKVRDGINGLFQL

AHLLYFFPILQTLMNVMPLPLLQVLNPPAFALASQKKELYEQGAAALEKANSKAGSGTLIDTLAAPSMPE

HMRSAHRLMNEGFALVIGGTETTARSLALGAWHLFTREDIRNKLREELKQVMPTPDSQPTWNDLEKLPYM

SGVISESLRLSTGIANRSSRVAPTEALVYKDYTIPPGTPVSETNYFTLMDPEIFSDPHAFDPERWIRAAA

KGQRLDRYLVNFSKGSRMCVGLNLAYAELFLVIATIVRRFDIELYETPKSNIDFARDFGTPYPDKGNFSV

RALVTGVISE

 

>CYP58F1  Aspergillus flavus

99% to CYP58F1  Aspergillus oryzae

AFL2G_09007

MALLVILGIGLVTYLVFLSIYRLYFHPLSHIPGPKLAAITHGYEFYHNIIRGGLFIWELERLHEVYGPIIRINPREVHIK

DPDYYDDIYASSARKREKDPVLVAQFGLEGSGFSAIDQETHHQRRAPVAKFFSKRAIENQEHLIRDSLNKLVGHFQEACR

AHTVVSLDAAFAALTSDVIHQYAYGFNPNNLDKEGFNAKVRDGINGLFQLAHLLYFFPILQTLMNVMPLPLLQVLNPPAF

ALASQKKELYEQGAAALEKANSKAGSGTLIDTLAAPSMPEHMRSAHRLMNEGFALVIGGTETTARSLALGAWHLFTREDI

RNKLREELKQVMPTPDSQPTWNDLEKLPYMSGVISESLRLSTGIANRSSRVAPTEALVYKDYTIPPGTPVSETNYFTLMD

PEIFPDPHAFDPERWIRAAAKGQRLDRYLVNFSKGSRMCVGLNLAYAELFLVIATIVRRFDIELYETPKSNIDFARDFGT

PYPDKGNFSVRALVTGVISE*

 

>CYP58F2 Aspergillus niger

fgenesh1_pm.C_scaffold_7000005|Aspni1

60% to CYP58F1

MFVYAWLGIGLLAYYVFRSVYLLYFHPLHKIPGPKLAAITYGYEFYYNVIKGGKFVWEIERLHKIYGTVIRINPDEVHIN

DPDYYEEIYTSRARRREKDPVQIAQFDLDGAVFSSITPETHRQRRSHLDKFFSKQAISNIEYLIHDSLDKIIKTYKEAYQ

SNNVVNLDAGFAGLTADVIYQYAFGFNPGNLDAERFNESVRDASNAFFRGIHILNFFPFIKVIMGLMPLGLLRLKNQTSV

QQDEXXXXXXXXX

KVSNSRGAILDAIAGPHMPAHMRTPVRLMNEGLALAIAGTETTARSLSVGTYHLLTRDDIRLKLREELKTVMPTPD

TRVGRTELEKLPYLSGVINESMRLSTGVSSRMPRVAPTEALVYKEHIIPPGNDPSSHVFILMDPNIFPDPHDFDPERWIR

AAEKGERLDRYLVNFSKGSRICLGMQLAYAELFIVIATLMRMFDMELADTPRETIEFARDFGTPYPEKGNLCVKARITIL

VQQ*

 

>CYP58F3 Aspergillus terreus

68% to CYP58F1

ATEG_04068.1

MASLILWGLASIIVWFALRSIYRLYFHPLSKFPGPKLAAITSGYEFYYNVVRRGMFIWEIERMHQQYGPIIRVSPREIHI

KDPDYYDEIYASSARKREKDPVVVAQFGLDGSAFSSVSPEDHRQRRAPLDKFFSKQAVTNMEHIIHECLDKLSRHLQDAC

ESHRAVSLDAGFAGLASDVIHQYAWGFNSGNLDAEDFNEHVRDGISGLFRMAHVMFFFPILQTITNAMPLWLLEKVNPFA

FAMASQKHDLYQRAVAALHNSGTKSGRGSIIDAIAGPSMPANMRTPERLMNEGFSLIIGGTETTARSLSLCFYNLITNEP

VRTKLREELRQVMPAPDSQPTWNQLEKLPYLSGVVLETLRLSTGIASRSPRIAPTEALEYKGYVIPPGTLVSETNYFILM

DPNIFPEPHVFDPERWMRAAAKGERLDRYLVNFSKGSRICLGMNLAYAELFLVIATMIRRFDFELYQTSKKDIDFVRDFG

TPYPDEGNFGIKAIVTRVLND*

 

>CYP58G1  Aspergillus oryzae

         GenEMBL BAE64022.1

         40% to 58C1, 41% to 58D1, 39% to 58B1, 35% to 58A3

         39% to 52E2, 41% to 58F1

         revised 3/18/2009

MNLPLIWIGLFTAVSYLVIRSIYRLYFHPLSNFPGPKLAAVTHLYEFYYDVVKGGKFIWEMQRMHDQY

GPIVRINPREIHIKDPYYFDPIYTSKGQAKDPYIVRTFATPLSTAATVEHDRHRYRRDLVNPFFSKRSVMGVDYIV

QDKVDKVCKRLTQVHERGTVVSLDDLFAALTADVISHYAYGESLGFLDTENLKNEFRDAVASAGLLCHFA

RFFFVVSMVAETMPALVEWMQPSSKGLWEAKRMIEQMARSSLEKDHEKNANSRKTIFDALCAESVRPEER

TVARVRDEAMVVFGAGTETTARVLATGSYYLYRDKPRLEKLRAEIETVMPDSTDHVSLTQLESLPYLTAV

INESLRMAHSVTMRLPRISPTPLAYKDYIIPPG

TPVSQSVYFMHMDPTLFPNPDSFNPERWLEASSKGERL

TKFLVPFSKGSRICLGMNLAYSELYQMFATLVRCFDLEIQTPPESVRITRDFIIGLPDDADYLKVHSLVT

NAL

 

>CYP58G1  Aspergillus flavus

99% to CYP58G1  Aspergillus oryzae

AFL2G_09004

MNLPLIWIGLFTAVSYLVIRSIYRLYFHPLSNFPGPKLAAVTHLYEFYYDVVKGGKFIWEMQRMHDQY

GPIVRINPRE

IHIKDPYYFDPIYTSKGQAKDPYIVRTFATPLSTAATVEHDRHRY

RRDLVNPFFSKRSVMGVDYIVQDKVDK

VCKRLTQVHEKGTVVSLDDLFAALTADVISHYAYGESLGFLDTE

NLKNEFRDAV

ASAGLLCHFARFFFVVSMVAETMPALVEWMQPSSKGLWEAKRMIEQMARSSLEKDREKNANSRKTIFDALCAESVRPEER

TVARVRDEAMVVFGAGTETTARVLATGSYYLYRDKPRLEKLRAEIETVMPDSTDHVSLTQLESLPYLTAVINESLRMAHS

VTMRLPRISPTPLAYKDYIIPPG

TPVSQSVYFMHMDPTLFPNPDY

FNPERWLEASSKGERLTKFLVPFSKGSRICLGMNL

AYSELYQMFATLVRRFDLEIQTPPESVRITRDFIIGLPDDADYLKVHSLVTNAL

 

>CYP58H1  Aspergillus oryzae

         GenEMBL BAE56719.1

         43% to 58B1, 44% to 58C1, 42% to 58D1, 38% to 58A3

         42% to 58G1, 40% to 58E2, 42% to 58F1

MDEFTISAGSLCLLSLVVGFIIQSVYRLFFHPLRKFPGPKLGAISHLYEFYYDVIYNGSYLFKIEQLHQKY (1)

GPVVRINPRELH

INDPYYYEQIYAGSSRIREKDPRFIGVFTTPLPMVATVGHEHHRIRRGLLSSYFSRRALKKAELIIDQKV

DRLLVRFHSAFKCHAVLPLQRVFAALAADIVSEYCYGASQGYLEQKVFQNQMIDAVNYVMSMCHINKSIP

IIPKLLRCVPVGLMEKLGLQMADVIGVRNLIRRQAAKSLDKEWLSHDTNMLSKNVFDAIAAADVAPQEKT

LRRLEEEGAALFGAGIETTARALTVAMFHLISDETMIRKLRDELKQVMLSPASRPTWAELEQLPYLTGVV

NESLRLSFGLVARSPRVSPIESLAYGEYVIPPG (0)

TPVSQSAYFVHMNPQV

FPEPESFNPERWIKAAEKGQYLSRFLVAFSKGSRQCLGMNLAYAELYLTLARIVRLVDMKLVGTTIDNIR

VGRDLGHPAPKAGNFKVKVEVMGIASKS

 

>CYP58H1  Aspergillus flavus

98% to CYP58H1  Aspergillus oryzae

AFL2G_07403

MDEFTISAGSLCLLLLVVGFSIQSIYRLLFHPLRKFPGPKLAAISHL

YEFYYDVICNGSYLFKIEQLHQKY (1)

GPVVRINPREL

HINDPYYYEQIYAGSSRIREKDPRFIGVFTTPLPMVATVGHEHHRVRRGLLSSYFSRRALKKAELIIDQKVDRLLVRFHS

AFKCHAVLPLQRVFAALAADIVSEYCYGASQGYLEQKVFQNQMIDAVNYVMSMCHINKSIPIIPKLLRCVPVGLMEKLGL

QMADVIGVRNLIRREAAKSLDKEWLSHDTNVLSKNVFDAIAAADVAPQEKTLRRLEEEGAALFGAGIETTARALTVAMFH

LISDETMIRKLRDELKQVMLSPASRPTWAELEQLPYLTGVVNESLRLSFGLVARSPRVSPIESLAYGEYVIPPGTPVSQS

AYFVHMNPQVFPEPESFNPERWIKAAEKGQYLSRFLVAFSKGSRQCLGMNLAYAELYLTLARIVRLVDMKLVGTTIDNIR

VGRDLGHPAPKAGNFEVKVEVMGIASKS*

 

>CYP58J1  Aspergillus oryzae

         GenEMBL BAE62179.1

         41% to 58C1 46% to 58C1, 44% to 58D1, 41% to 58B1,

         40% to 58A3, 43% to 58E2, 42% to 58F1, 41% to 58G1,

         41% to 58H1

MDSPRLWTGLAGLITYLIVISVYRLFSHPLRNIPGPKLAAVTHLYEWYYDLFLGGKYLFEIERMHERYGP

IV RINPPEIHINDPKYYDEIYASGTHRRNKDAEFVSFTGLLLSSASTTDHDLHRYRRGLMNNFFSKKSVR

GISYFVEEKVHNLMQRFEAFYRCNKVVRLDDAFAAMTSDVITHYCYGKSWDYLDYANLRTDVRKAVRDLT

CSVHFNRIFPIFLAVLKKLPLRWLYAIHPGRSVVLDIQKTIYEQSAEAIHGDKYKIGHNDAVDKHKTIYD

QLTDPSIPAEERSLQRLQDEGLLLISVGTETTARALTTACFHIASDDQLRTRLREELRTVLPTPTSSVTW

SELEKLPYL (0)

TGTVNESLRLGGFLTTRSPRIAPDEPLTYKEYTIPPS (0)

TPVSSSSYFGHKNPNIFPEPEKFSPERWISAGRNNDHLFKYITSFSRGSRICAGMNLAF

LELYMTLAYFVRRFDVELVDTTVEDM KIVRDMRVGFTHRGEPTVYGRIVRVYED

 

>CYP58J1 Aspergillus flavus

98% to CYP58J1 Aspergillus oryzae

AFL2G_06095

MDSPRLWTGLAGLITYLIVISVYRLFSHPLRNIPGPKLAAVTHLYEWYYDLFLGGKYLFEIERMHERY (1)

GPIMRINPPEIHINDPKYYDEIYASGTHRRNKDAEFVSFTGLLLSSASTTDHDLHRYRRGLMNNFFSKKSVRGISYFVEEKVH

NLMQRFEAFYRCNKVVRLDDAFAAMTSDVITHYCYGKSWDYLDYANLRTDVRKAVHDLTCSVHFNRIFPIFLAVLKKLPL

RWLYAIHPGRSVVLDIQKTIYEQSAEAIHGDKYKIGHNDAVDKHKTIYDQLTDPSIPAEERSLQRLQDEGLLLISVGTET

TARALTTACFHIASDDQLRTRLREELRTVLPTPTSSVTWSELEKLPYLTGTVNESLRLGGFLTTRSPRIAPDEPLTYKEY

TIPPGTPVSSSSYFGHKNPNIFPEPERFSPERWISAGRSNDHLFKYITSFSRGSRICAGMNLAFLELYMTLAYFVRRFDV

ELVDTTVEDM RIVRDMGVGFTHRGEPTVYGRIVRVCED*

 

>CYP58K1  Aspergillus oryzae

         GenEMBL BAE63021.1

         41% to 58J1, 38% to 58A1, 40% to 58D2, 39% to 58C1,

         38% to 58B1, 42% to 58J1, 37% to 58H1, 37% to 58G1,

         39% to 58F1, 39% to 58E2

MVMDQAWTWMALVAIAGLFWITRIFYRLYFHPLAKIPGPKLAAASHLLEFYYDVILGGKFLFQVEKMHQK

YGPIVRINPKEVHIIDPTFYNEIYASGMRKRDKYEGFVRSLAADESTVSTVGSEKHRFRRSILQNFFSKR

SVMEFSSAIGERVEKLMRRLEVFEKTQTPVALDVVFSALTSDMITYICYGKDWKFLDHKDFNCDIHQAGV

DFANFFHFNRFFPWVFMTLRALSPRMLALLIPGRAATFKFQESLLKHAIEMAANEQSDAPSKETEKSRPN

VISNLINPSIPYMERSRRRLEDEVITILVAGTEAPVKVLSMAMYYLGSEPAIGEKLRAELKTILPARTST

ATYAELEKLPYLHGVVYESLRISDSVIARFPRIAPTETLRYKDHILPPGTPMSCSSYFISRNHDLFPNPE

KFDPERWIHAAEKGENLKQHLTAFTKGSRICLGINLTIAELFLTIAHMCRRYHILLHNTEPEDVCTTSDL

LAGYTRRGVLKVHAKLKAVRE

 

>CYP58L1 Aspergillus niger

e_gw1.12.119.1|Aspni1

49% to CYP58D1, 49% to e_gw1.8.187.1

MYTILLPLLVVICVSVRAIYRIYFHPLSKIPGSKLAAATHLHEFYHDVIRGGKFIWEVEKMHRIYGPIVRINPREVHIAD

PSMYDEIYAGCGRKREKDPCMVSAYASSYAALATVDHDLHRARRAPLNRFFSKKAVIELSGVVQQKVSFLAWHLERVNAE

QAVLVLSTAFTALTGDIITHYLYGKDNGYLRERDLVKRNIVWDILAEGTSACHLFRFFPFIPTLTKALPIQLMRLVRPEL

SNVYAMQEQIARQSDKALASERGAKRRTIYLALSDPSVPARERSVARLRDESFIVLLGGTESTAATLTFATYHLLRNKDM

YFKLRQELQQVMPEPTSEADWAQLERLPYLTAVINEALRLGAAALRPARVAPTETLYYKGYEIPPGTPVSTISYFVHRNP

AIFPDPESFNPERWIQAANRGDNLTRYLVPFTRGSRICIGMNLAYAELYMTLAAIVRRFDLELCETHPKDMEFTREMVVQ

RPEKGVWTLKVRVVGINET*

 

>CYP58M1 Aspergillus niger

e_gw1.8.187.1|Aspni1

51% to CYP58K1

MALDTSHIWLALVGIASFYVLRSIYRLYFHPLSGIPGPKLAAVTHLYEFYYDVVIHGRFLFQIEKMHQQYGPIVRINPRE

VHISDPSFYDEIYASSTRKRERDPLAYGAFALPYS MIGTMGHEHHRFRRNILNDFFSKRSVLAISPFIEERVEKLRGRFE

GFYRDQSVVNLSDAFAALTSDVITYYCYGKRWGFIEDKDFRSDVHGGNEENTGLAHFNRFFPIVPETMRKIPLHLMALCL

PGKAGMLQFQGSILSTISENTHDKASEKEDRTIFKRLTDPNLPVAERSQRRIEDEVFTLLGAGTETTASTLMIMTYYVSR

DRSIRDKLRSELKQVLPTPTSTATWPELEKLPYLTAVVNESLRLSYGLIMRLPRVAPTETLKYKDYVLPPGTPMSTSTWF

VHRDPSLFPEPDRFDPERWIKAAEEGVNLTRYIVSFMRGSRACIGMNLAYIELYLTIAHVVRRFNFDVEITSPESMRITR

DRIVPGTEKGLMRVYAKVTDMMED*

 

>CYP58M2 Neosartorya fischeri

55% to CYP58M1

NFIA_057570

Note: this seq has no ortholog in A. fumigatus

MMLSSSWLLPALAYIFLCFLVQSIYRLFFHPLSRYPGPKLTAATFLYEFYYDVIKGGQFLFQIERMHERYGPVVRINPRE

LHIKDPSFYDTVYAGGTHQRDKDPGWVHISGAPLSTFSTVSHDHHRFRRSILSDFFSKKSVTALSPIIEERVLKLMQRFQ

EAYDSRSAIRVDAAFAGLTADVITHYAYGKSWGFLEDPQFRSNIRAAVNETTNAVHWFRFFPFLVTIFRTIPAQIMCFVQ

PSKTALFEFQKSIFDHAAQPKSKRNDSARTIFHRLTDPSIPAEERSLARIQDEAFIILAAGTETTARTLTIATFYMANEP

RIWKKLRDELQSSVLPTLSSTATWAELEKLPYLNAVINEALRLSYGAVIRLPRITPTETLKYKDYVIPPGTPVSMSTYFV

HQDPSIFPDPQTFRPERWIKASDQPEHLTKYIVSFTRGSRICLGMNLAYIELFMTIAYMVRRFDMVLHETTFEDIRIVRD

YVLGLSKHGDLRVFTKVTNMLQE*

 

>CYP58M3 Aspergillus terreus

55% to CYP58M1

ATEG_02828.1

MSLHSSMASFSLLGLAYLVLRSIYRLYFHPLRHIPGPRLAAISSLYEFYYDVVCQGRYLWEIERLHAIY (1)

GPIVRINPREVHIIDPSFYDEIYAPSSRRRDKDAKWVPTFGLSQS

MLSTVGHQQHRFRRSLLSSFFSKKSVLQIGPLIDQQREKLFQHLVNFHRTQEVVRLDSAFVALTSDIISAYCYGESGNFL

DDPSFRSDIRASVVDAASICHLARFLPFLQPLIQSVPEGILRALMPGKTALLDFEHSLASKASKALHEGKPSAAAEETIY

DRLTDKSVPPEERSLCRIQDESSLVLAAGTETTARILTIAIYYLSRDQSMLDKLRAELKQALPTPTSLTTWVQLEKLPYL

TAVVRESLRLGFGITCRLPRVAPEEVLRYKGYEIPPGTAMSTCTMFVHRNPEIFPDPDRFDPERWIEGGQLNNKLTKYIV

SFTRGSRICLGINLAYSELYLLIAHLARRFTFEIYDTSEDDVRVTRDLMVGYTRRGDLQVYAKVAEVVQE*

 

>CYP58M4 Aspergillus terreus

57% to CYP58M1

ATEG_03531.1

METQTLWGILLAFSVYQFFKTIYRLYFHPLSKIPGPRLTAATHLYEFYYDVILGGKFLFQIEKMHQTYGPIVRINPREVH

ISDPTFYDEIYASSTVKRDKDPHLVRIFGAPFSMVGTVGHEHHRFRRSLLNNFFSKRSVLELSPLVTERVQKLMERFEGL

HRSNAVVSLDDAFSALTADVITAYCYGKCWMFLEDESFRNDIRKTVSDMTSYMHINRFFPLVLSAMRAIPLWLLAVLQPG

KSSLLKFQEDIFKETVDSVRDRGKSLSGEKSNPARSTIYDKLSDPSVPPEERTLQRIQDEGVILLSAGTETTGRALCMAA

FHLAYDKSITNRLRDELRQVMPRPTDTVSWTELEKLPYLNGVVYEALRLSYGLIIRLPRISPTDALQYKDHIIPPGTPMS

SSSYIVHRDESIFPNADKFDPERWIRAKNEGKNLSRYLVSFTRGSRTCIGMNLAYMELYLTLASFVRRFDIELHDTTPED

LRFTHDLGIGFTRRGDMQVYAKVTGVVEN*

 

>CYP58N1 Aspergillus niger

e_gw1.9.613.1|Aspni1

42% to CYP58C1, 39% to e_gw1.8.187.1

MDYYKALIVLIVYLCLRTIYRLALHPLRGIPGPKLAASTSLYEFYYDAIKGGKYIWEVERMHRQYGKSFTEAILSSLNLT

RGKGPIVRISPREVHIKDPYFYDEIYTSSSIRKRDKDSKLVASFCAPTASFSTVDHNLHRSRRAVISHLFTRKAVVSHEF

LIQDNVGKLASRLESIHKKGSVFSLKIAAAALISDVLAQYMYGTSTHYLDDPTFRSESVASATELASKIHILRHIPVIAT

IFQLLPLALLRPFRLLSDFCDFRLWLRKQSQDALQGKGGSTVRRGPVFDALLRANVPAEEKTLQRLEDESFTLFSAGSET

VSQALSVIFFHLLKNAEFIKTLRKELKQVMPMPTDLATWSELEKLPFLTAVITEALRLSGTSGRLPRVSPTEPLIYGKYI

IPAGTPVGQSNYFAHRDPAMYPNPNAFDPYRWIHAEDENTNPKPFYAPFSKGSRQCIGINLAYATLYLTVATLIRRFDME

ISDSTEDELRTARDFLIPFPEKG

 

>CYP58P1 Aspergillus clavatus

49% to CYP58H1

ACLA_013370

MASFAMDKDQILVGVLLYISGVIVYRLFLHPLSRFPGPKLAAISSLPEFYHDVLRGGRYIWEIERMHEKYGPIVRINPRQ

LHIKDPCFYDEIYAGGSRRRDKYAEYVNALAADNSSAT TVDHTHHRIRRGFISPYFSKRSVLQFEATISEKLAMLTARLE

EASHAGSVLNLQHVFAALTADIIMQYAYGETHGYLAHESFRNDLVDAVATQLDLLHVNRFFPGLVRLFKDAPPWLLRAVG

LKIADVVEVKLWIRELAAKALARKGRAGGRARETIFDALTAETVPPEEKTLDRLEVESTVIFAAGTDTTARALAVASFHL

MQNQDVLRTLREELKRVMPTPTTSVSWLQLEQLPYLSGV VNESLRLSYALVTRLPRVAPDETLKYGDYMIPPGTPISQSA

YFVHQDPTIFPEPQRFHPERWIRAAEKGENLKRFLVSFNKGSRQCLGINLAYAELYRTIAAVARRFDMELYETTLEDILV

NRDLGFGQPETGRFQVQARVTGIVKE*

 

>CYP58Q1 Aspergillus terreus

49% to CYP58M1, 48% to CYP58P1

ATEG_02820.1

MAVTLPLIVALAFLWFGSKAIYRLYWHPLSHIPGPKRAAISHLYEFYYDVIRGGTYLF

EVEKMHQKY (1)

GPIVRINPREVHISDPDFYDEIYAPAARVRWKDPKQVPMIGAPFSMGA

MGATWHHGHHRFRRSLIKNHFSRQSIEKITTTIRKKAEILCGRLEEASAIARPLNVSDAFAALTSDIIAGYAFDIDLGFL

EDPLFRNDVYRSALELDMLCHVLKFFPFLLKPVRWLPSSVTEVLGLQNKSLGSLQEVIDQRIISSLRTTGQSGHAQEKER

DSIFTSLCHQEVPPEERTLERLRDESLVLLIAGAEATARALAVFVYYMATGSINVQKLRQELRQVMPTCTSQASWAELEHLPYL (0)

SGVVNESLRLSFGAIGRLSRIAPTEDLRYKAHPIPRMTPMSSSAYLIHRNADVFPE

PDCFRPERWIGAAASGVNLHRYLVAFSRGTRQCIGINL

AQCQLYFTIAAVARRFDFELFETTEEDIRVARDYITAFPKDGLFDVKVTVRSRLGD*

 

>CYP58-un1 Aspergillus niger

gw1.19.191.1|Aspni1

43% to CYP58J1 pseudogene fragment C-term, 52% to CYP58M1

TPMGSSSDFIRRDPDNFPSLNNFTSTRWLMENGITIHSTRYLVSFSKGSRGRIEM ()

NLPYFELYLTVAYLVR

 

>CYP59A1 Aspergillus nidulans AACD01000132.1 Aspergillus nidulans revised

stcS Aspergillus nidulans L27825

MYTTIITAVCVLFALHLLDSFYQARQEP

MPSFSLLTGHFGALKQTIDGMPPNATLHSIMLKLSQKFRSGMFY

INMWPFSGTWLVVATPSGAAQIQSLNLSKPNILRRPLETITGGPSLMSMHGETWKRWR

ALFNPGFNPNYLIGLAPLIADEVVVFCEQLRQKARTGTVFQLEPLTLRLTVDTICSVT

LYVVTPVGRWPFLTPDLEIHSSTTKLRTTPLPQRCNGRSNGPRLELPSTPLRRYLTVR

PLVMWYNNRLMNRFIDQEVDRAYREQSGRQSKSVISLALRDYMKEKDGSLEDFKRRVA

PQLRVFLFAGRDTTSSTLLYAFYLLSRHPEALAKVRLEHDQVFGPYHQQVHEKIHQDA

KLLNQLPYTTAVLKETLRLFPPSASMREGGPGVEITDDNGQVYPTAGCNVWTLTVALH

HNSAHWAEAESFIPERWLVGSDHPLYPAKGAWRAFEFGPRSCIGQTLAMLELRVALAM

TLREFDIAPAYDKWDHIYPNDAVKEFNGHRAYQAEKGGGGAHPADGMPCLVTFRV

 

>CYP59A3     Aspergillus parasiticus aflatoxin pathway gene cluster

            GenEMBL AY371490

MPEFSLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFSSGIFY

INMWPFSGTWMVVSTPSAASQIQKLNLTKPVILRRPLETVTGGPSMMSMHGETWKKWR

ALFNPGFNPGYIIGLAPNITDEVATFWLSCAKGQQGEVFPLESLTTRLTVDSICSVVL

DTQLHHQIKDHPLATALQRQIDWTTFGTTFNPLKRYLTIRPLVLWYNNKVMDRIIGGE

VDRACRTPPDHPSKSVISLALREYLQEQASTNSTRSLAEFKRLVAPQLRVFLFAGRNT

TSSTLIYSYYLLAQHPEVLAKIRAEHEDVLGADPAEAQGRIKEDVQLLNKLPYTTAVI

KETLRLFPPSASMREGRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDYWDQVENFI

PERWLVGPEDPLYPVKGAWRAFEFGPRSCIGQTLAMLELRIALAMTIRQFDITPAYDE

WDSIHPATTAKEVNGHRAYQAERGAGGAHTADGFPCRVKERC

 

>CYP59A4  Aspergillus oryzae

         GenEMBL BAE59520.1 also BAE71324.1

         72% to 59A1

MPEFQLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFYINMWPFSGTWMIVSTPSAASQIQKLN

LSKPAILRRPLEMVTGGPSMMSMHGETWKKWRALFNPGFNPAYIIGLAPNISDEVAIFCAQLRKIAQQGE

VFPLESLTTRLTVDSICSVVLDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPFKRYLTIRPLVLWYNNK

FMDRIIDGEVDRAYCTPPGHPSKSVISLALREYLQEQASNNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS

TLIYTYYLLAQHPEALAKIRAEHGDVLGADPAEAQGRIKEDVQLLNKLPYTTAVIKETLRLFPPSASMRE

GRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDYWDQVENFIPERWLVGPEDPLYPVKGAWRAFEFGPR

SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVK

ERC

 

>CYP59A5  Aspergillus oryzae

         GenEMBL BAE48804.1

         92% to 59A4, 71% to 59A1

MPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISKQFQSGIFYINMWPFSGTWMIVSTPSAATQIQKLN

LTKPAILRQPPETVTGGPSMMTMHGETWKKWRALFNPGFNPAYIIGLAPNITDEVATFCAQLRKKAQQGE

VFPLESLTTRLTVNSICSVVLDTQLHHQIKDYPLATALQRQINWTSFGTTFNPLKRYFTIRPLVLWYNNK

VMDRIIGGEVDRAYRTPPDHPSKSVISLALREYLQEQASSNSTRSLAEFKRLVAPQLRVFLFAGRNTTSS

TLIYTYYLLAQHPDILAKIRAEHEDVLGVNPEEVQGRIKEDAQLLNKLPYTTAVIKETLRLFPPSASMRE

GRPDAEIIGENGQRYPTVGCNVWTLTVALHHNSDHWNQVESFTPERWLVGPEDPLYPVKGAWRAFEFGPR

SCIGQTLAMLELRIALAMTIRQFDITPAYDEWDSIHPATTSKEVNGHRAYQAERGAGGAHPADGFPCRVK

ERF

 

>CYP59A5  Aspergillus flavus

96% to CYP59A5  Aspergillus oryzae

AFL2G_07219

MYLSLLIALGVVSFIRFLTAFYQARQEPMPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISKQFQSGIFYINMWPFSG

TWMIVSTPSAATQIQKLNLTKPAILRQPLETVTGGPSMMTMHGETWKKWRALFNPGFNPAYIIGLAPNITDEVATFCAQL

RKKAQQGEVFPLESLTTRLTVDSICSVVLDTQLHHQIKDHPLATALQRQINWTSFGTTFNPLKRYFTIRPLVLWYNNKVM

DRIIGGEVDRAYRTPPDHPSKSVISLALREYLQEQASSNSTRSLAEFKRLVAPQLRVFLFAGRNTTSSTLIYTYYLLAQH

PEVLAKIRAEHEDVLGADPAEAQGRIKEDVQLLNKLPYTTAVIKETLRLFPPSASMREGRPDAEIIGADGQRYPTMGCNV

WTLTVALHHNSDHWNQVESFIPERWLVGPEDPLYPVKGAWRAFEFGPRSCIGQTLAMLELRIALAMTIRQFDITPAYDEW

DSIHPAPTAKEVNGHRAYQAERGAGGAHPADGFPCRVKERF

 

>CYP59A6 Aspergillus nomius isolate AN13137

AY510454.1

join(49221..49702,49768..50767)

94% to CYP59A5 Aspergillus flavus

gene VerA of aflatoxin biosynthesis

MPEFKLLAGHFGTLKKTIQGMPSDATLHSIMLKISQQFPSGIFY

INMWPFSGTWMIVSAPSAASQIQKLNLTKPAILRRPLETVTGGPSMMSMHGETWKKWR

ALFNPGFNPAYIIGLAPNIADEVATFCTQLRKKAQQAEVFPLESLTTRLTVDTICSVV

LDTQLHHQIKDHPLATALQRQIDWTSFGTTFNPVKRYLTIRPLVLWYNNKVMDRIIDS

EVDRAYRTPPDRPSKSVISLALREYLKEQSSSSSTRSLAEFKRLVAPQLRVFLFAGRN

TTSSTLIYTYYLLAQHPEVLARIRAEHEDVLGGDPADAQGRIKEDVQLLNKLTYTTAV

IKETLRLFPPSASMREGRPDAEIIGEDGQRYPTVGCNVWTLTVALHHNSDHWQQVESF

IPERWLVGPEDPLYPVKGAWRAFEFGPRSCIGQTLAMLELRIALAMTIRQFDITPAYD

EWDSIHPATTAREVNGHRAYQAERGAGGAHPADGFPCRVKERC*

 

>CYP59A7 Aspergillus ochraceoroseus strain SRRC 1432

AY092402.3

join(9134..9615,9669..10671)

gene StcS/AflN in aflatoxin/sterigmatocystin biosynthesis

76% to CYP59A5 Aspergillus flavus

MPEFKLLAGHFAALKQTIQGMPPNATLHSIMLKLSQQFPSGIFY

INLWPFSGTWMIVATMTAASQIQSLNLTKPAILRRPLETITGGPSLISMHGETWKRWR

ALFNPGFNPAYLIGLAPNIADEVAVFRNQLRKKAQQGDIFSLEPLTLRLTVDTICSVA

LDARLFHQQKDHPLAVALQRQIEWASFGTTFNPVKRYLTIRPLVLWYNSRLMNRLIGK

EVDRVHNARPGYSSKSVISLALKQYLKEQSNGDTRQSLEAFKRQVAPQLRVFLFAGRD

TTSSTLLYTFYLLAKHPEILERVRREHDEVFGTDIKKAQSCIAEDPQLLNKLPYTLAV

IKETLRLFPPSASMREGRPDVEIVGDDGRRYPTAGCNVWTLTVALHHNSNHWPEVESF

IPERWLVGHEDPLYPAKGAWRAFEFGPHSCIGQTLALLELRIALAMTLREFNITPAYD

EWDRLHPRDTIKEVNGHRAYQMEKGGGGAHPADGLPCRVTLRTT*

 

>CYP59B1X  # Fusarium graminearum (Gibberella zeae PH-1)

          GenEMBL AACM01000117.1

          52362-54017 (+) strand, one intron

          renamed CYP5071A1

 

>CYP59C1 Aspergillus nidulans AN5766.1 44% to 59A1 586clan?

Revised 7/18/07

MAVTNLVLTLSIPLLTVIGYGLYCGFQHRRKINELRKRGIPMPKDWSWLT

GHLLSLKKYTDRLPADAHVLLPTHELAVEFADTEMFLMDTWPVFPALVMV

YDPDAALQISTKYNLPKSAIFPSLMHPITGGPSMISMNDAEWKKWRSIFN

PGFSAGNMVDQVSTVVDSVQVFCDILREKAGTGLVHLDDLTTRLTMEVIL

KVTLDMDSNYQRSDNEMVHALNTITKWHSFWDPRVRANPLRPLVQKYYGR

VMDRCIRKELDKRFAEMQQGQRSASTSKRVKSVIALALEAYLADYHQKDG

KASKLDDNFAQYATHQIRLFLFAGNDTTSSSIVYVYHMLSKHPKALARVR

QEHDRIFGTLPSAAPQLLKSNPALLNQCPYTLAVIKETLRLFPPASTTRE

GRDGVTLTDRLGNSYPLGHTIGAEIIHPTIHKNPRLWPQAEEFIPERWLV

DPGHELYPSPAAWRPFEHGPRNCIGQTLVYNEMRIVIVMTARTFNIRPAY

DEWDAMHAAKEGLL (0)

TVHGERAYQTEKAGTHPADGYPCHVALCSGSPIS*

 

>CYP59C2 Aspergillus niger

e_gw1.2.1390.1|Aspni1

63% to CYP59C1

MAFINLLLTICLPVVVSVVAYGLHALYQHRSKINRLRKQGIAMPPGWNWLTGHMFVLSEYLNRLPPDANVVLATQELAWE

YTDTEIFLLDVWPMSPAQYIVFYPEAASQATTKFNLPKTVIHDRFMRPITGGPSMISVGNAEWKPWRSIFNPGFSAGSMQ

DLLPEVIKSVSVFCDKLREKADSGEMFALDDLTTRLTMDVILKVTLDADVDHQNSDNIIATALGQITKWHSFWDPRVRAN

PLRPLVQGYYGRVADKWIRKALADRFSEMKQVKRDASIRSKRAKSILTLALEAYLENKNEEISEATELDDDFAKHVTYQI

RLFLFAGNDTTSSSIAYVYHLLSKHPEALTQVREEHDRIFGPDPSAAVQLLCEQPSLLNKCPYTMAVIKETLRLYTPAST

MRQGIDGLSLTDRHGNRYPLADDLSVSVLHQAVHLNPRVWPRAREFLPERWLVDADHELYPDPAAYRPFEQGPRNCIGQT

LVYNEMRTVLVMTARTFEIKPAYDEWDATKAKQKGWFDQIAESVGFQNKKPKLLHGERAYQSEKAGTHPVDGYPCRVSLV

A*

 

>CYP59D1  Aspergillus oryzae

         GenEMBL BAE66084.1

         AP007175.1

         55% TO 59D2, 41% to 59A1, 41% to 59C1

470087 MVIAVSDLVGSYGARVALVLALVIVLRFLQEMLKVRLLFYRLRKQ

GLPMPKWNFAAGNLQMLPDLMKRHP

KGSQQSEAFTLLSYEFASSDNCFYIDVWPFTKPLLVVNSP DLAVQACQTYALPKPPVLAKFFNPFAGGPS

IFTTNGPEWKRNRG LFNPAFSTSNILQHTPHI 470671

VEEAEEYVEILREHARKGDTFTLDKMTCDYVLDIIGRVAM (2)

KARLHSQRGRNPVAAALRSQVEWHCQDEQMNPFIRWNPMRPIMQWCNGRTM

NQYIGAELDKRYEAWTQNKPSTRANSIIDIVLAEYMSTRPVRAALDPEFKSWAT

IQLRTFLFAGHDSTAATIVYSIYLLSKHPEILSKVRTEHDEVFGSDISAAAGILKQHPEL

INRLPYTLAVIKETLRLFPAASALREGQPGVYLQDKNGTKYPTEGLCIWIIHGGIQRNPN

YWPDPHAFKPERWLVGPDHPLYPPKGAWRPFEQGLRDCIGQALALLDVKITLVLTLREFD

FQDQYAHWDRLHPRSGPKTVFGERAYQIPQ GGSHPVDGLPCRVSLRNQITK*

 

>CYP59D1  Aspergillus flavus

99% to CYP59D1  Aspergillus oryzae

AFL2G_11439

MVIAVSDLVGSYGARVALVLALVIVLRFLQEMLKVRLLFYRLRKQGLPMPKWNFAAGNLQMLPDLMKRHPKGSQQSEAFT

LLSYEFASSDNCFYIDVWPFTKPLLVVNSP

(seq gap)

LFNPAFSASNILQ

HTPHIVEEAEEYVEILREHARKGDTFTLDKMTCDYVLDIIGRVAMKARLHSQRG

RNPVAAALRSQVEWHCQDEQMNPFIRWNPMRPIMQWCNGRTMNQYIGAELDKRYEAWTQNKPSTRANSIIDIVLAEYMST

RPVRAALDPEFKSWATIQLRTFLFAGHDSTAATIVYSIYLLSKHPEILSKVRTEHDEVFGSDISAAAGILKQHPELINRL

PYTLAVIKETLRLFPAASALREGQPGVYLQDKNGTKYPTEGLCIWIIHGGIQRNPNYWPDPHAFKPERWLVGPDHPLYPP

KGAWRPFEQGLRDCIGQALALLDVKITLVLTLREFDFQDQYAHWDRLHPRSGPKTVFGERAYQIPQGGSHPVDGLPCRVSLRNQITK*

 

>CYP59D2  Aspergillus oryzae

         GenEMBL BAE58153.1, AP007155.1

         37% to 59A1, 37% to 59C1

2825205 MGANTLGWDGLTSRIAVAVATVCLTSFVYKLIKMRLMFYRLRKKGL (0) 2825068

2825008 PTPPWNPILGNLAVMAQLQKKWPSDSREAESFALLSTEAPGCEAGFYVDVWPFSIP

MLVVTSPALAVQACQTYDLPKPDVLQPFINPMAGGSDNLFVSNGAHWKQARELFNHGFSMAAAMSHMTYI

LEEAQVFVQMLKDHARKGDTFSLDALTCRYVMDIIGNVA LNTRFRFQEQHNPIAAAMRDTIELECGIETS

NFLSRWNPRRLYRQWQNGRTMDYLIGVELDKRYKEWRETAKSSSHPRTQSIMDMVIAEYMKTRPQAQQQQ

ELDPEFKRWATIQIRLFLFVGHDSEATTIIYSLYLLSKNPGVLIKVRAEHDRVFGAGVSSAYDVLTDHPE

KINQLSYTHAVIKETLRLFPPANGLRGGLPGVSLRDEQGRIFPTEGCAIWIVHTAVHRNPSSWPQPHAFI

PDRWLVEPGHPLYPPAGGWRPFEQGPRNCIGQNISLLGIKASLAMLVRQFDFHDAYAEYDRLHPSTGLKT

MFGERAYMIQKGAGHPAQGFPCKVTLR

 

>CYP59D2  Aspergillus flavus

99% to CYP59D2  Aspergillus oryzae

MGANTLGWDGLTSRIAVAVATVCLTSFVYKLIKMRLMFYRLRKKGL (0)

PTPPWNPILGNLAVMAQLQKKGPS

DSREAESFALLSTEAPGCEAGFYVDVWPFSIPMLVVTSPALAVQACQTYDLPKPDVLQPFINPMAGGSDNLFVSNGAHWK

QARELFNHGFSMAAAMSHMTYILEEAQVFVQMLKDHARKGDTFSLDALTCRYVMDIIGNVALNTRFRFQEQHNPIAAAMR

DTIELECGIETSNFLSRWNPRRLYRQWQNGRTMDYLIGVELDKRYKEWRETAKSSSHPRTQSIMDMVIAEYMKTRPQAQQ

QQELDPEFKRWATIQIRLFLFVGHDSEATTIIYSLYLLSKNPEVLIKVRAEHDRVFGAGVSSAYDVLTDHPEKINQLSYT

HAVIKETLRLFPPANGLRGGLPGVSLRDEQGRIFPTEGCAIWIVHTAVHRNPSSWPQPHAFIPDRWLVEPGHPLYPPAGG

WRPFEQGPRNCIGQNISLLGIKASLAMLVRQFDFHDAYAEYDRLHPSTGLKTMFGERAYMIQKGAGHPAQGFPCKVTLR*

 

>CYP59D3 Aspergillus niger

e_gw1.12.714.1|Aspni1

63% to CYP59D2

MPPWDPIFGHLRIMPGLAKKCPSDALQSQTFAVLSMEYPGLQNGFYIDVWPFMCPMFVCTTPPLAVQACQTYSLAKPTEL

LAPFINPMAGGDNFFTTNGAAWKRDRDLFNHAFSMAAVLGHVDYILEEAEIYVEILREHAKKGDTFSMDDLACNYMMDVV

GNVALNTRFNYQTGHNPIAAAMRSTIDLECGREGENNPLRRWNIHRLYRQWRNSQIMDHHIGLELEKRYQEYLQQNQSTK

PRGKSIMDIVIAEYMKHRPASQTHTATLDPEFKAWAIIQIRLFLFVGHDSTAVTIIYCLYLLSKYPDALVKIRAEHEQIF

GPKTTSVATQIKEHPEKTNQMPYTTAVIKEALRLFPPANGLRFGRPGVSLTDTHSSDGRTFPVDDCAVWIVHTAIQRNPG

YWPHAHKFVPDRWLVEPGHELYPPTGGWRPFERGARDCVGQNMALLAIKISLAMVVREFDFDSQYEEWDRMNPASGAVRT

MFGERVYMVAKGAAHPAQGYPCKVRLAA*

 

>CYP59E1 Mgr034 Mycosphaerella graminicola

39% to 59C1, 9/10 top hits are CYP59, but only 34% to 59D3

N-term is short

MAISNLKLYQEFVIKHSIPKHPLVAEFMENFGGQSNLVSSEGATWKRWRSVFNPGFSNSHIMSQVSSIVDVAQTFCEIMN

DHAKNNTIFRMETATTKLTVDVIGKIVLDLDLNSQKGDNILADTFTSQVRWQSMGAQYQPSELWDIRRPIVQRYNNWVMS

RYLKEKVQERFATREGRGKSKHVVDLALEVYLKEVKGTNSDGGNIKSLDAEFMEAVIANMKTFVFAGHDTTSTVIGCAYY

YLSRNPEALTKLRNELDEVFGPDPSTVAQQLKDDPLLLNKMDYAVAVCKETLRLMPPASTVRAAAPGYRLIDPDTGEHIP

TEHMLVWPSATGIHRHPKYFPDPHTFKPSRFLPGDDTGSNTAAWIPFSKGPRNCIGQELAMIEAKIVLAMTVRTWDFVPA

YDELEKLEGDGTGYPNLKTGILEQYGERMYQIQLGTAKPAEGLPCRLRFTGQ

 

>CYP59E2 Mycosphaerella fijiensis

61% to CYP59E1 Mycosphaerella graminicola

e_gw1.7.674.1

MQLQPLTGTHLIWAVGLVLAAYILRFLQRLHFHRTLVNGLPGPPHSYAFGSLISMGKVAAKQPPNAAPQALMQCLKDAYD

LPDAFYFDSWPLGPPIMVITNPDMANQITIKYNPPKHPLVAEFMVNFGGKYDLVSEEGAVWKKWRSAFNPGFAPGHLITQ

VSTIVDECQVFCDIMEKRARNNELFRMEPAATKLTVNIIGKIVLDLDLNAQHGKNVLVDSFMSQIRWQKIGLQYQPSELW

DIRRPIIQRYNNWRMHRWLSARLDERFAGLEARGKSKHVIDLALEAYLKEIKGQKGSVDASKIKGLDPDFKEAAISNMKT

FTFAGHDTTSSAICYSFYYLSKYPETLAKIRKEHEEIFGPDPDVVAQKLKSDPHLLSKMEYTLAVTKEVLRMQPPASTIR

LGQANLTLHNPDTGENMPTNHFMLWPVNVGMHRNPRNWPNPDIFDPERFIPGSAVYAENNKDAWVPFSKGVRNCIGQELA

IMEAKVILAMIVRKFDFISAYNELEKLVGDGSSYPNYLSGVREQFGERAYQVQLGTAKPAEGMPCRMKLAKR*

 

>CYP60A1 Aspergillus parasiticus L40839

MHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDKGLRHERSLI

ERSIDLLITQLHENCGQGSGLALWFNWATFDIIGDLAFGDSFGCLENVQTHPWIASIQ

GNVKLIPILNAFRRYRLDGLLRLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDI

WDAVLAQKPDGEPPMTRDEMISNASAIVLAGSETSATLLSICTWLLLKNPSHLHQLTS

RIRSQFTHASEIDSQSVSRVEGLQAVLEESLRLYPPVPMQSNRIVPQAGAYIAGGWVP

GGTSVGLQQFVACRSSSNFHRPDEFLPERWQGQGEFAHDRREVSQPFSIGPRNCIGRQ

LAYVEMRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNRCGLRWNLAMSSSS

NYSL

 

>CYP60A2 stcF Aspergillus nidulans U34740 AN7818.1 53 clan

revised 7/19/07

MILPLILVLYLLSTAAYRLWLHPVRNYPGPCWWAVWRVPYLKGT

IRGTIVRDIQRLHNQYGPVVRIAPDELSYITPEAAKPIYTSSPEFPKDPMLLPPFHNG

APGILAADYAHHRRYRRLLASAFSEKGLRAQQGMIQSHIDRLMTRLQGNCSSGSLDMT

VWFNWATFDIIGDLAFGEPFGCLERMETNPWIASIQGNVKSIPILNALRRYRLDRLIE

FLAPPRLLEMRRRNAQFTAEKVDRRLKHATTTRGDLWDSVLADPPDGEPPMSRAEMVS

NASAIVLAGSETSATTLSGCLWLLLTNPEYLQQLTERIRARFSTATVIDAQTVTQIQG

LQAVLDESLRLYPAVPMQSNRIVPPPGARLAGSWVPGGTSVAVQQFAACRSPTNFHRP

DEFIPERWEKEGEFINDRREASQPFSIGPRNCIGRQLALAEMRLILVHLLWHFDIELD

RRRMENMDWMAVQGI

WILWDKKPLWVVLKNRST*

 

>CYP60A3 Aspergillus parasiticus avnA U62774

MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRW

WAIWRGPYILSNIRGNLVRDLQRLHQQFGPVVRIAPNELSFIVPEAASPIYTSNPEFP

KDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDKGLRHERSLIERSIDLLITQL

HENCGQGPLDLALWFNWATFDIIGDLAFGDSFGCLENVQTHPWIASIQGNVKLIPILN

AFRRYRLDGLLRLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPD

GEPPMTRDEMISNASAIVLAGSETSATLLSGCTWLLLKNPSHLHQLTSRIRSQFTHAS

EIDSQSVSRVEGLQAVLEESLRLYPPVPMQSNRIVPQAGAYIAGGWVPGGTSVGLQQF

VACRSSSNFHRPDEFLPERWQGQGEFAHDRREVSQPFSIGPRNCIGRQLAYVEMRLIL

VKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKKPLWVTLEPRNE

 

>CYP60A5  Aspergillus oryzae

         GenEMBL BAE71325.1, BAC45242.1, BAE59521.1

         95% to CYP60A3

MGGDGWPSDGHILLLIVLTVLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRGNLVRDLQRLHQ

QFGHVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDK

GLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGNSFGCLDNVQTHPWISSIQGN

VKLIPILNAFRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPP

MSREEMISNTSAIVLAGSETSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAI

LEESLRLYPPVPMQSNRIVPQSGAYIAGGWVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAH

DRREVSQPFSIGPRNCIGRQLAYVETRLILVKLLWHFDLRLDTTRMKDTDWLAEQGIWILWDKNPLWVNL

EPRNE

 

>CYP60A5  Aspergillus flavus

98% to CYP60A5  Aspergillus oryzae

AFL2G_07217 revised

Bottom HALF (two adjacent P450s in this gene model CYP60B3 and CYP60A5)

MGGDGWPSDGHILLLIVLT

VLTPPSLALYRLWIHPLRSYPGPRWWAIWRGPYILSNIRG

NLVRDLQRLHQQFGSVVRIAPNELSFIVPEAASPIYTSNPEFPKDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSD

KGLRQQRSLIERSVNLLITRLHENCGQGPLDLTLWFNWATFDIIGDLAFGDSFGCLDNVQTHPWISSIQGNVKLIPILNA

FRRYRLDGLLQLLGSRKLLEQRRRNAQFTTDQVDRRLKNSSTPRGDIWDAVLAQKPDGEPPMSREEMISNASAIVLAGSE

TSATLLSGCTWLLLKNPGHLHQLTSRIRSQFTHASEIDSQSVSRVEGLQAILEESLRLYPPVPMQSNRIVPQSGAYIAGG

WVPGGTSVGLQQFVACRSSSNFHRPEEFLPERWQGQGEFAHDRREVSQPFSIGPRNCIGRQLAYVEMRLILVKLLWHFNL

RLDTTQMKDTDWLAEQGIWILWDKKPLWVTLEPRNE

 

>CYP60A6 Aspergillus nomius isolate AN13137

AY510454.1

complement(join(51845..52180,52258..53352,53400..53456))

92% to CYP60A3

gene AvnA of aflatoxin biosynthesis

MGVDGWLSDGHFRLLILLIVLTPPSLAVYRLWIHPLRSYPGPRW

WAIWRGPYILSNTRGSLVRDLQRLHQQFGPVVRIAPNELSFIAPEAAAPIYTSNPEFS

KDPMHLPPFHNGTPGILAADHAHHRRYRRLLAFSFSDKGLRQQRGLIERSVNLLITQF

HENCGQGPLDLTLWFNWATFDIIGDLAFGDSFGCLDNVQTHPWIASIQGNVKLIPILN

GLRRYRLDGLLRLLGSRKLLEQRRRNAQFTTDQVDRRLQNSSTPRGDIWDAVLAQKLD

GEPPMSRAEMISNASAIVLAGSETSATLLSGCTWLLLKNPEHLHQLTSRIRSEFSHAS

EIDSQSVSRVEGLQAVLEESLRLYPPVPMQSNRIVPSTGAHIAGGWVPGGTSVGLQQF

VACRSPSNFHRPDEFLPERWQGQGEFAHDRREVSQPFSIGPRNCIGRQLAYVEMRLIL

VKLLWHFDLRLDTTRMKGTDWLAEQGIWILWDKKPLWVTLEPRDK

 

>CYP60A7 Aspergillus ochraceoroseus strain SRRC 1432

AY092402.3

complement(join(29656..30030,30172..31266,31338..31385))

gene AflG in aflatoxin/sterigmatocystin biosynthesis

79% to CYP60A3 Aspergillus parasiticus avnA

gene model revised at one intron boundary

MDVLRSFPLQLLGLRDFLILGSLGAYRLWLHPLRSYPGPRWWAL

WRVPYLQSTIRGTIVRDLQRLHEQYGPVVRIAPDELSFINPEAAKPIYTSNPEFSKDP

MHLPPFHNGVPGILAADHAHHRRYRRLLAFSFSDKGLKAQQGMIQQYIDQLIVRLHEN

CSTGPVDMTLWYNWATFDIIGDLAFGESFGCLEKVETHPWIASIQGNVKAIPILNALR

RYRLSGVLGLLAPRNLLEMRRRNAQFTADKVDRRLQHPGTARGDLWDSVLADNPNGES

PMSRDEMVSNASAIVLAGSETSATLLSGCTWLLLKNPEYLNTLTERVRSQFARAADID

AQTVTQVEGLQAVLEESLRLYPPVPMQSNRIVPPQGAQIAGMWVPGG (0)

TSVAVQQFVACRSADNFHRPNEFLPQRWQGQGEFANDRREASQPFSIGPRNCIGRQLAYAEMRL

ILVHVLWHFNLRLDQGRMKDADWLAEQGIWILWDKNHCGLCWNRGREKVEEDDAT*

 

>CYP60B1 stcL Aspergillus nidulans U34740, AN7813.1, revised 7/18/07

MAFLSLPILTALGA

VVYVLFQLVYNLYFHPLRDYPGPLLW

RASSLPWKLTLLRGTMHHDLMRHHQTYGDTVRIKPDEISYANGQAWRDIHAHVPGRPE

FLKDPVRLPLAPNGVMSILVSDTRNHARFRSLFGHAFSDKGLRAQEPTIARYADLLVE

VLREVADTGKSVEMVRYFNMAIFDSIGALSFGESFDSLRNRELHPWVDTIHKNLKSVA

ISHVLRSMGVEFLAPYLMPAELRGKRQENYTYAIEKLKKRMQKTGDQGDFWDRVIVKS

ADGNQSGDGMSYGEMINNAAVMVVAGSETTSSALCGCTYLLCKFDKMDKAVAEVRGAF

AAADQIDLVSVSRLPYLTAVIDETLRMYPSVPGQPPRVVPEGGAIVCGRFVPAETRVG

VSHLGAYYAPYNFSHADKFIPERHLAGAKLEEPFRHDNYAAYQPWSVGVRNCIGRNLA

YAEVRLTLAKLLWHFDISLDEERTGNFLDQKIWSIWAKRELYLEIRTREF

 

>CYP60B2     Aspergillus ochraceoroseus strain SRRC 1432

            GenEMBL AY092402.3

            complement(join(46781..47113,47181..48332))

            Cary,J.W., Beltz,S.B., Bennett,C.A. and Klich,M.A.

            Molecular Characterization of the Aflatoxin Biosynthetic Cluster

            from Aspergillus ochraceoroseus.

            Unpublished

            function="desaturation of aflatoxin precursor VER B to VER A

            84% to CYP60B1 Aspergillus nidulans StcL

            probable ortholog of CYP60B1

            gene AflL/StcL

MALPTLPVLAVLIGASYILVQLVYNLYFHPLRDYPGPLLWRASS

LPWKFTLLRGTMHHDLMRFHQKYGDTVRIKPDEISFANAQAWRDIHAHVPGRPEFLKD

PVRLPLAPIGVMSILVSVTRFLARFRSVFGLAFSVKGLRAQEPTIVQYADLLVEVLRE

VADTGKSVEMVHYFNMAIFDSIGALSFGESFDSLKNRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYTYAIEKLKRRMQMTGDQGDFWDRVIVKSADGN

QSGDGMSPGEMLNNAAVMVVAGSETTSSALCGTTYLLCQSGKMEKAVAEIRNAFPTPD

KIDLVTVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGKFLPAETRVGVSHL

GAYYADYNFTRPEKFIPERHLQKMEEPFKHDNYGAYQPWSVGVRNCIGRNLAYAEVRL

TLAKLLWHFDITLDEAKTGNFLDQKIWSIWANGELYMSFKARKV

 

>CYP60B3  Aspergillus oryzae

         GenEMBL BAE71326.1, BAC45243.1, BAE59522.1

         81% TO 60B1

         probable ortholog of CYP60B1

MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSLPWKIALLRGTMHHDLMRFHQKYGDT

VRVKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGL

RTQESTIVQYADLLVEVLREMANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNL

KSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDLGDFWDKVLVKSADDNQRGD

GMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPEDIDLISVSHLPYLAAV

IDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHLGAYFADYNFTHADKFIPERHLQKTEEPY

KYDNYGAYQPWSVGLRNCIGRNLAYAEVRLTLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIK

TRGTSSSSPQ

 

>CYP60B3v1  Aspergillus flavus

97% to CYP60B3  Aspergillus oryzae

AFL2G_07217 revised

TOP HALF (two P450s in this gene model CYP60B3 and CYP60A5

MYFLSLPALAIIVPVGYVLLHLGYNLFFHPLRGYPGPLLWRASSL

PWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKDPVRLPLAPNGVMSILVSDTKNHA

RFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLREVADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDA

IHKNLKSVAISHVLRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDNQRGDGMSAG

EMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKSTAEIRKNFASPEDIDLISVSHLPYLTAVIDETLRMYPAVPGQP

PRVVPAGGATVCGRFVPEETRVGVSHLGTYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGLRNCIGRNLAY

AEVRLTLAKLLWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSTPQ*

 

>CYP60B3v2  Aspergillus flavus strain SRRC 141

            GenEMBL AF106960

            Bhatnagar,D., Cary,J.W., Ehrlich,K.C., Cleveland,T.E. and

            Payne,G.A.

            Molecular characterization of an aflatoxin B2 producing mutant

            strain of Aspergillus flavus

            Unpublished

            verB gene 85% identical to 60B2

            97% to 60B3v1 ortholog

            99% to CYP60B3v3  Aspergillus flavus strain SRRC 1007

            formerly CYP60B4v1

MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS

LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD

PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE

VANTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN

QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIKKATAEIRKSFASPE

DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL

GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL

TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFMKTRGTSSSSPQ

 

>CYP60B3v3  Aspergillus flavus strain SRRC 1007

            GenEMBL AF106959

            formerly CYP60B4v2

MYFLSLPALAIVIPVGYVLFQLGYNLFFHPLRGYPGPLLWRASS

LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD

PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE

VANTGCSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN

QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKVEKATGEIRKSFASPE

DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGRFVPEETRVGVSHL

GAYFADYNFTHADKFIPERHLQKTEEPYKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL

TLAKLLWHFDFTLDVGKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ

 

>CYP60B3    Aspergillus parasiticus strain RH1

            GenEMBL AF106958

            formerly CYP60B4v3

MYFLSLPSLVIVIPVGYLLFHLGYNLFFHPLRGYPGPLLWRASS

LPWKIALLRGTMHHDLMRFHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD

PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE

VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN

QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPE

DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPASGATVCGRFVPEETRVGVSHL

ATYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL

TLAKLLWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ

 

>CYP60B3    Aspergillus parasiticus aflatoxin pathway gene cluster

            GenEMBL AY371490

            formerly CYP60B4v3

MYFLSLPSLVIVIPVGYLLFHLGYNLFFHPLRGYPGPLLWRASS

LPWKIALLRGTMHHDLMRFHQKYGDTVRIKPDEISYANAQAWRDIHAHVPGRPEFLKD

PVRLPLAPNGVMSILVSDTKNHARFRSLFGHAFSDKGLRTQESTIVQYADLLVEVLRE

VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMKMEGDQGDFWDKVLVKSADDN

QRGDGMSAGEMLNNAAVMVVAGSETTASALSGAMYLLCLSGKIEKATAEIRKSFASPE

DIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPASGATVCGRFVPEETRVGVSHL

ATYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGLRNCIGRNLAYAEVRL

TLAKLLWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMFIKTRGTSSSSPQ

 

>CYP60B3 Aspergillus nomius

AY510454

complement(join(53898..54266,54322..55473))

gene VerB of aflatoxin biosynthesis

94% to CYP60B3 Aspergillus flavus
MYFLSLSVLALVTLVSYVLFHLGYNLFLHPLRGYPGPLLWRASS

LPWKIALLRGTMHHDLMRYHQKYGDTVRIKPDEISYANAEAWRDIHAHVPGRPEFLKD

PVRLPLAPNGVMSILVSDTRNHARFRSLFGHAFSDKGLRTQESTILQYADLLVEVLRE

VADTGRSAEMVYYFNMAIFDSIGALSFGESFDSLKSRQLHPWVDAIHKNLKSVAISHV

LRSMGIEFLTPYVLPKELRGKRQENYSYAVEKLNKRMRMEGDQGDFWDKVLVKSADDN

QRGDGMSAGEMLNNAAVMVVAGSETTASALSGSMYLLCLSGKIEKATAEIRKSFASPE

EIDLISVSHLPYLTAVIDETLRMYPAVPGQPPRVVPAGGATVCGKFVPEETRVGVSHL

GAYFADYNFTHADKFIPERHLQKTEEPFKYDNYGAYQPWSVGIRNCIGRNLAYAEVRL

TLAKILWHFDFTLDVDKTGNFLDQKIWSIWAKRELYMTLKSRDTSCSWPSTSSSPQ*

 

>CYP61A1 Yarrowia lipolytica

64% to CYP61A1   Saccharomyces paradoxus

CAG84120.1

MNATQPESHIVQWLTWTQDTIGFGTLAATVLLIALLYDQFSYVKSKGSIAGPPLKMWPIIGPFLDSINPDFEKYKAQWAS

GALSCVSVFHKFVVLASDRDLTRKIFNSSAYVKPCVVDVAIKILRPTNWVFLDGKAHVDYRRGLNGLFTNRALSMYLPIQ

EGIYDTYFDKFVEMSKDGHVPYMPIFREINCALSLRTFCGNYITEEQIKEIAHDYYLVTAALELVNFPIILPFTKAWYGK

KAADKVMDIFAKCAQMAKDHIKAGGDVTCTMDAWIAIMNGRDFDSDHGTVPNKKIRKFTNKEISETIFTFLFASQDASSS

ATTWLFQIVADRPDVYAKIREEQLAVRGGDPNVPLSLELTEKMTYTNMVVKECLRLRPPVIMVPYVAKKDFPISDTYTVP

KGSMIIPTVYPSLHDPEVYERPDEFVPERWLPDGDGTKNAKNWLVFGTGPHYCLGQKYALMNFTNMIGKACMNLDFTHKV

TPLSEKVRVFATIFPDDDCLLQFKKRE

 

>CYP61A1 S. cerevisiae bakers yeast Z49211 Z71257

MSSVAENIIQHATHNSTLHQLAKDQPSVGVTTAFSILDTLKSMS

YLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWPIIGPFLESLDPKFEEYKAKWAS

GPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT

DYRKSLNGLFTKQALAQYLPSLEQIMDKYMDKFVRLSKENNYEPQVFFHEMREILCAL

SLNSFCGNYITEDQVRKIADDYYLVTAALELVNFPIIIPYTKTWYGKKTADMAMKIFE

NCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDDSRIYHREFTNKEISEAVFTFL

FASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMV

IKETLRYRPPVLMVPYVVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIP

ERWVEGSKASEAKKNWLVFGCGPHVCLGQTYVMITFAALLGKFALYTDFHHTVTPLSE

KIKVFATIFPKDDLLLTFKKRDPITGEVFE

 

>CYP61A1 Saccharomyces paradoxus

98% to CYP61A1 S. cerevisiae Spar_17277

MSSVAENIIQQATHNSTLHQLAKDHSPVGATTAFRILNTLKSMSYLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWP

IIGPFLESLDPKFEEYKAKWASGPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT

DYRKSLNGLFTKQALAQYLPSLEQIMDKYMDKFVRLSKENNYEPQVFFHEMREILCALSLNSFCGNYITEDQVRKIADDY

YLVTAALELVNFPIIIPYTKTWYGKKTADMAMKIFENCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDDSRIYHRE

FTNKEISEAVFTFLFASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMVIKETLRYR

PPVLMVPYVVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIPERWVEGSKASEAKKNWLVFGCGPHVCLGQT

YVMITFAALLGKFALYTDFHHKVTPLSEKIKVFATIFPKDDLLLTFKKRDPITGEVFE

 

>CYP61A1 Saccharomyces mikatae

97% to CYP61A1 S. cerevisiae Smik_16456

MSSVAENIIQQASHNSTLQQLAKDQSSVGITTAFSILDTLKSMSYLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWP

IIGPFLESLDPKFEEYKAKWASGPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT

DYRKSLNGLFTKQALAQYLPSLELIMDKYMEKFVRLSKENNYEPQVFFHEMREILCALSLNSFCGNYITEDQVRKIADDY

YLVTAALELVNFPIIIPYTKTWYGKKTADMAMKIFENCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDESRIYHRE

FTNKEISEAVFTFLFASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMVIKETLRYR

PPVLMVPYVVKKNFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDEFIPERWVEGSKASEAKKNWLVFGCGSHVCLGQT

YVMITFAALLGKFALNTDFHHKVTPLSEKIKVFATIFPKDDLLLTFKKRDPITGEVFE

 

>CYP61A1 Saccharomyces kudriavzevii

97% to CYP61A1 S. cerevisiae Skud_Contig2054.8

MSSIAENIIQQATHNSTLHQLAKDESSLGVTTAFSILETIKSMSYLKIFATLICILLVWDQVAYQIKKGSIAGPKFKFWP

IIGPFLESLDPKFEEYKAKWASGPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT

DYRKSLNGLFTKQALAQYLPSLEQIMDKYMDKFVRLSKENNYEPQVFFHEMREILCALSLNSFCGNYITEDQVRKIADDY

YLVTAALELVNFPIIIPYSKTWYGKKTADMAMKIFENCAQMAKDHIAAGGKPVCVMDAWCKLMHDAKNSNDDESRIYHRE

FTNKEISEAVFTFLFASQDASSSLACWLFQIVADRPDVLAKIREEQLAVRNNDMSTELNLDLIEKMKYTNMVIKETLRYR

PPVLMVPYVVKKNFPVSPSYTAPKGAMLIPTLYPALHDPEVYENPDDFIPERWVEGSKATEAKKNWLVFGCGPHVCLGQT

YVMITFAALLGKFALYTDFHHKVTPLSEKIKVFATIFPKDDLLLTFKKRDPITGEVFE

 

>CYP61A1 Saccharomyces castellii

83% to CYP61A1 S. cerevisiae Scas_Contig709.53

METIMDNSTMEFVQQQTPSTLISTIQQLSYWKIFVTFICIVLVWDQISYQIKKGSIAGPRFKIWPIIGPFLESLDPKFEE

YKAKWDSGALSCVSIFHKFVIISSTRDISRKIFQSSKFVKPCVVDVAIKILRPTNWVFLDGKAHIDYRKSLNGLFTKTAL

AQYLPSQEQVMDKYLQKFIEISKENNYEPQVFFHEMREILCALSLRAFCGEYITEDQIRKIADDYYLVTAALELVNFPII

IPFTKTWYGKRTADAAMKIFEQCAQMSKDYIAKGGKPICVMDAWCKLMHDAKTKNDEDSRILHREFSNKEISEAIFTFLF

ASQDASSSLACWLFQIVADRPDILEKIRKEQMEVRNNDMSTELNIELIDKMKYTNMVIKETLRYRPPVLMVPYVVKEKFP

VTPNYTAPKGSMLIPTLYPSLHDPEVYENPDEFIPERWEEGSPASEAKKNWLVFGCGPHVCLGQTYVMITFAALLGKFAL

YTDFEHKITPLSEKIKVFATIFPKDDLLMTFKKRDPITGKVFE

 

>CYP61A1 Saccharomyces bayanus

93% to CYP61A1 S. cerevisiae Sbay_17696

MSSVAENIIQQAANNSTLHQLAKGGSSLGFTKAFNVLDALKSMSYLKMFATLICILLVWDQLAYQIKKGSIAGPKFKFWP

IIGPFLESLDPKFEEYKAKWASGPLSCVSIFHKFVVIASTRDLARKILQSSKFVKPCVVDVAVKILRPCNWVFLDGKAHT

DYRKSLNGLFTKHALAQYLPSLELIMDKYMEKFVRLSKDNNYEPQVFFHEMREILCALSLNSFCGNYITEDQVRKIADDY

YLVTAALELVNFPIIIPYSKTWYGKKTADMAMKIFENCAQMAKDHIAAGGEPVCVMDAWCKLMHDAKNSNDDDARIFHRE

FTNKEMSEAVFTFLFASQDASSSLACWLFQIVADRPDVLEKIREEQLAVRNNDMSTELTLDVIEKMKYTNMVIKETLRYR

PPVLMVPYVVKKNFPVSSNYTAPKGAMLIPTLYPALHDPEVYENPDDFIPERWEEGSKASMAKKNWLVFGCGSHVCLGQT

YVMITFAALLGKFALSTDFHHKVTPLSEKIKVFATIFPKDDLLLTFKKRDPITGEVFE

 

>CYP61A1 Saccharomyces kluyveri

79% to CYP61A1 S. cerevisiae Sklu_Contig2168.4

MSTVVKYAQNAASNSILNSGNNATTTATNAVATTVQNVIDSVQSISYWKLFVTIICVGLVWDQVSYQIKKGSIAGPKWKV

WPVIGPFLESLDPKFEEYKAKWDSGPLSCVSIFHKFVVIASTRDLARKILQSPKFVKPCVVDVAIKILRPSNWVFLDGKQ

HVDYRKSLNGLFTKSALAQYLPSQENVMNKYLEKFVELSKENKYEPQVFFHEMREIMCALSLKAFCGDYITEDQIRKVAD

DYYLVTAALELVNFPIILPFTKTWYGKKTADMTMKIFEQCAQMAKDHIASGGPTTCVMDAWCKLMHDAKNQNDADSRLLH

REFSNKEISEAIFTFLFASQDASSSLACWLFQIVADRPDVLEKIREEQLKVRENDPTKALSLDLIEEMKYTNMVVKESLR

YRPPVLMVPYVVKQKFPIAPNYSAPKGSMLIPTLYPALHDPEVYENPDEFIPERWVEGSPANQAKRNWLVFGSGPHVCLG

QTYVMMTFTALIGKFAMYTDFEHKVTPLSEKIKVFATIFPKDDLLLSFKKRDPLTGENII

 

>CYP61A1 Kluyveromyces waltii

82% to CYP61A1 S. cerevisiae kwal_095-g1.1 C-term part only

MCALSLKAFCGDYITESQIRKVADDYYLVTAALELVNFPIILPFTKTWYGQRTADMTMKIFEKCAQMAKDHIAAGGKPKC

VMDAWCELMHNAKNKNDEDSRLFHREFTNREISEAVFTFLFASQDASSSLACWLFQIVADRPDVMQKIREEQLRVRNNDP

SKPLSLDLIDEMKYTYMVVKETLRYRPPVIMVPYVVKNKFPIAPNYTAPKGAMLIPTLYPALHDPEVYDDPEEFIPERWV

EGSPANEAKKNWLVFGSGSHVCLGQTYVMMTFTALIGKFAMYTNWEHKVTPL

 

>CYP61A1 Ashbya gossypii ATCC 10895

75% to CYP61A1 S. cerevisiae NP_982999.1

MDAIGFGEQAGWLWRALAALWAWRVLAALVLVLVADQAAYRRNKGRIAGPAWKMWPLIGPFLESLDPKFEVYQAKWASGP

LSCVSIFHKFVVIASTRDLTRKILQSPKYVKPCLVDVAKKILRPTNWVFLDGQAHLDYRRSLNGLFTRDALARYLPSQEA

VIDRYMAKFVAFSRETDYEHRVFFHEMREMLCALSLKAFCGSYITDDQVRKIADDYYLVTAALELVNFPLILPFTKTWYG

KRTADMAMKIFERCAQEAKQHIAAGGEPTCVVDAWCKLMLDAKAKDDADSRLLHRKFTNREMSEALFTFLFASQDASSSL

ACWLFQIVADRPDVLAKIREEQLAVRRNDPSVPLSLELIDQMKYTHMAVKECLRYRPPVLMVPYQVHEAFAVSADYTAPK

GSMLVATMYPALHDPAVYENPDDYIPERWDGEQSPANQAKKNWLVFGSGPHVCLGQKYVFMTFTALIGKFALFTEFDHKV

TSLSEKIKVFATIFPQDDLLMSFKKRDPLTGQTTE

 

>CYP61A1 Pichia stipitis

82% to CYP61A1   Debaryomyces hansenii

JGI model estExt_genewise1_human.C_chr_6.10410

MSFNTTSPLAVGYKNAQQYLDIETFITKAQETYSSLTWIQLIFSVIAIILSYDQIMYQIRKGSIAGPRFKIWPIIGPFLE

SLDPKFEEYKAKWDSGELSCVSIFHKFVVIASSRDLARKILASPKYVKPCVVDVAIKILRPTNWVFLDGKQHTDYRRSLN

GLFSQKALEIYIPVQEKYMDIYLDKFTKFDGPREFFPEFRELLCALSLRTFCGDYITEEQIALIADNYYRITAALELVNF

PIIIPYTKTWYGKKIADDTMKIFEKCAAMAKVHINEEAGKPACVMDEWIYLMKDAREKHSEDPEAKLLIREFSNKEISEA

IFTFLFASQDASSSLACWLFQIVADRPDVVEKIREEQLAVRNNDPSVPLSLDLINEMSYTNNVVKESLRYRPPVLMVPYV

VKQSFPVTETYTAPKGSMIIPTLYPALHDPEVYEDPDTFIPERWENATGDMYKRNWLVFGTGPHVCLGKNYVLMLFTGML

GKFVMNADLIHHKTALSEEIKVFATIFPKDDLIMEWKSRNPLEA

 

>CYP61A1 Debaryomyces hansenii

80% to CYP61A1   Candida lusitaniae

CAG86871.1

MSTNSTSEIVDNLSGSFGDTSFFIKTANDLYMNMSWLQLIITTIVLGLSYDQIMYQIRKGSIVGPRLKVWPIIGPFLESL

DPKFEEYKAKWDSGPLSCVSIFHKFVVIASSRDLARKILGSPKYVKPCVVDVAIKILRPTNWVFLDGKAHSDYRKSLNGL

FSQKALELYIPVQEKYMDLYLEKFVTLDGPTEFFPQFRELLCALSLRTFCGDYITEEQISLITDNYFRITAALELVNFPI

IIPYTKTWYGKKIADETMKIFESCAGMSKKHINEDNGKPGCVMDEWIYLMKDAREKHSDDPESKLLIREFSNREISEVIF

TFLFASQDASSSLACWLFQVVADRPDIVAKIREEQLRVRDQDPSKPLNLELINEMTYTNNVVKESLRYRPPVLMVPYVVK

QSFPVTDTYTAPKGSMIIPTLYPALHDPEVYEDPDSFIPERWENATGDMNKRNWLVFGTGPHICLGKTYVLMLFTSMLGK

FVMNSDIIHYPTPLSEEIKVFATIFPKDDLILEWKRRNPLQV

 

>CYP61A1 Lodderomyces elongisporus

84% to CYP61A2   C.albicans

LELG_05188

MSEFNVSTPLVDRLLNEQAKSFISSNQQINYLYNLYQTSSWLQIIATFIVSVLLYDQVRYQLNKGSIAGPKFKVWPIIGP

FLESLDPKFEEYKSKWDSGELSCVSIFHKFVVIASSRDLARKILSSPKYVKPCVVDVAIKILRPTNWVFLDGKAHTDYRR

SLNGLFSQKALEIYIGVQEKYMDIYLERFCKYEGPREFFPEFRELLCALSLRTFCGDYITEDQIALVADNYYKVTAALEL

VNFPIILPYTKTWYGKKIADETMKIFANCSAMAKVHIENGGSPTCIMDEWIFLMKEAREKHLDDPESRLLIREFSDKEIS

EVIFTFLFASQDASSSLACWLFQIVADRPDIVAKIREEQLRVRNNDPSVPLDLNLINEMTYTNNVVKESLRYRPPVLMVP

YVVKQPFPVTESYTAPKGSMIIPTLYPALHDPEVYDDPDTFIPERWETASGDMYKRNWLVFGTGPHVCLGKNYVLMLFTG

MLGKFVMNSDIVHHKTALSEEIKVFATIFPKDDLILEWHKRDPLAV

 

>CYP61A1 Candida guilliermondii

79% to CYP61A1   Candida dublinensis

PGUG_05085.1

MDVNSTNQVTDMYSTPIGGTVGSYVNDVFTNLTWVQIFCTTVVLIFTYDQVMYQLRKGSIAGPRVKMWPIIGPFLESLDP

KFEEYMAKWNSGPLSCVSIFHKFVVIASDRDLTRKILASPKYVKPCVVDVAVKILRPSNWVFLDGKAHSDYRRSLNGLFR

SKALEIYIPVQEKYMDKYLEKFTNFDGPRKFFPEFRELLCALSLRTFCGDYITEEQIKLIADNYYRITAALELVNFPIII

PYTKTWYGKMIADDTMKIFESCAAMAKKHINEENGTPTCIMDEWISLMKDAREKHSEDAASKLLIREFSNREISEAIFTF

LFASQDASSSLACWLFQIVADRPDVVKKIREEQLKVRDNDPSKPLSYDLINEMTYTNFVVRESLRYRPPVLMVPYVVKQK

FPVTETYSAPKGSMLIPTLYPALHDPEVYDDPDSFIPERWYDASKEMTNRNWLVFGTGPHVCLGQNYVLMLFTGMLGKYM

MNADLIHHKTPLSEEIKVFATIFPKDDLIMEWHSRDPSKL

 

>CYP61A1 Candida parapsilosis

79% to CYP61A2   C.albicans, N-term does not match

CPAG_03443

MDSFNQTVELAGSSHTNQIFNFLTSNKQINYFYQLYLSASWIQIVIASVV

LILSYDQVKYQLNKGSIAGPKFKIWPIIGP

FLESLDPKFEEYKAKWDSGALSCVSIFHKFVVIASTRDLARKILSSPKYVKPCVVDVAIKILRPTNWVFLDGKEHTDYRR

SLNGLFSQKALEVYIPVQEKYMDIYLERYLNYNGPRKFFPEFRELLCALSLRTFCGDYITEDQISLIADNYFRITAALEL

VNFPIIIPYTKTWYGKKIADDTMKIFENCAAKAKVHIGQGGKPTCVMDEWIYLMKEAKDKHLDDPESKLLIREFSNREIS

ETIFTFLFASQDASSSLACWLFQIVADRPDVVEKIREEQLRVRNGDMNVRLDLELIQKMKYTNNTVRETLRYRPPVLMVP

YVVKQNFPITESYTAPKGSMVIPTLYPALHDPEVFEDAESFIPERWENPTGDMDKRNWLPFGVGPHVCLGQKYVLMLFTG

MLGKFLVGADLVHHKTDLSEEIKVFATIFPKDDLILEFKRRV

 

>CYP61A1 Candida lusitaniae

78% to CYP61A2   C.albicans

CLUG_04246.1

MSLNSSSPIVDAVSSKALETSSIVARIAETWHSLSWLQIFITFVIGVLSYDQISYQIKKGSIAGPRFKVWPIIGPFLESL

DPKFEEYKAKWDSGPLSCVSIFHKFVVIASTRELARKILSSPKYVKPCVVDVAVKILRPTNWVFLDGKAHVDYRKSLNGL

FSLKALEIYIPVLETYMDKYLDKFVTYDAPRKFFPEFRELLCALSLRTFCGDYITEDQIKEIADNYFRITAALELVNFPI

IIPYTKTWYGKKIADETMKVFEDCAAEAKIHINEKNGKPTCVMDEWIFLMKKARESHAEDSESKLLIREFSNKEISEAIF

TFLFASQDASSSLACWLFQLVADRPDVAAKVREEQMRIRNNDPSVPLSLDLINEMTYTNHVVKEALRYRPPVLMVPYVVK

QPFPVTPEYTAPKGSMVIPTLYPALHDPEVYPDPDSFIPERWENATGDMNKRSWLVFGSGPHVCLGQQYVMMLFTGMLGK

YLMNSEIKHHVTPLSEEIKVFATIFPKDDLILEWEKRDPLSATS

 

>CYP61A1 Candida dublinensis

96% to CYP61A2   C.albicans

cdub_7-g12.1

MNSTEVDNLPLHEQLTSFVELAIAKATGSPITTLFTIIFLILLYDQLSYQINKGSIAGPRFKFYPIIGPFLESLDPKFEE

YKAKWDSGELSCVSIFHKFVVIASSRDLARKILSSPKYVKPCVVDVAVKILRPSNWVFLDGKQHTDYRRSLNGLFSSKAL

EIYIPVQEKYMDIYLEKFCNYDGPREFFPEFRELLCALSLRTFCGDYITDDQIAVVADNYYRVTAALELVNFPIIIPYTK

TWYGKKIADDSMKVFENCAAMAKKHINENNGTPTCVMDEWIHLMKEAREKHSEDPDSKLLVREFSNREISEVIFTFLFAS

QDASSSLACWLFQIVADRPDVVAKIREEQLRVRNNNPDLRLSLDLINQMTYTNNVVKETLRYRPPVLMVPYVVKKSFPVT

ESYTAPKGAMIIPTLYPALHDPEVYDEPDSFIPERWENASGDMYKRNWLVFGTGPHVCLGKNYVLMLFTGMLGKFVMNSD

MIHHKTDLSEEIKVFATIFPKDDLILEWKKRDPLKSL

 

>CYP61A1 Candida glabrata CBS138

81% to CYP61A1 S. cerevisiae CAG62657.1

MASVVDQVHMSMNATAHAAGAASAGAVMAAPSLLDKLQGMSYLQMFVTLICGVLVWDQVSYQMKKGNIAGPRFKVYPIIG

PFLESLDPKFEEYKAKWDSGPLSCVSIFHKFVIIASTRDLARKVLQAPKYVKPCVVDVAVKILRPSNWVFLDGKAHTDYR

KSLNGLFTKTALAQYLPPLEELMDKYIEKFVELSKENNYEPQIFFHEMREILCALSLRSFCGDYISEDQIRKIADDYYLV

TAALELVNFPIILPFTKTWYGKRTADMAMKIFESCAQRAKDHIAAGGKPICVMDNWCKLMHDAKNRTDDDSRLLHREFTN

REISEAVFTFLFASQDASSSLACWLFQIVADRPDVLAKIREEQLRVREGDINKRLDIDLVDKMEYTHMVVKETLRYRPPV

LMVPYVVKNKFPVVPDYQAPKGSMLIATLYPALHDPEVYENPDDFIPERWVEGSPANEAKKNWLVFGCGPHVCLGQTYVM

ITMTALIGKFALFTDFKHKVTPLSEKIKVFATIFPKDDLLLSFKKRDPVTGEIKE

 

>CYP61A1 Kluyveromyces polysporus

Kpol_1056p10

86% to CYP61A1 S. cerevisiae

MSHPDMAMAINNNLNNMTSIDSSSLLVSIQSLSYWKIFVTLISVLLVWDQISYQIKKGSIAGPAFKFYPIIGPFLESLDP

KFEEYKAKWDSGELSCVSIFHKFVVIASTRDLARKILQAPKFVKPCVVDVAVKILRPSNWVFLDGKAHVDYRKSLNGLFT

KTALAQYLPSQEEVIDKYMEKFVEYSKKNNYEPQVFFHEMREILCALSLQSFCGDYITEDQIRKIADDYYLVTAALELVN

FPIILPYTKTWYGKRTADAAMKIFESCAQRAKDHIAAGGKPICVMDAWCKLMHDAKDRNDSESRLFHREFSNKEISEAVF

TFLFASQDASSSLACWLFQIVADRPDILQKIREEQMAVRNNDLNTPLTIELIEKMTYTNMVIKETLRYRPPVLMVPYVVK

QKFPVSPNYTAPKGAMLIPTLYPALHDPEVYENPDDFIPERWVEGSKASEAKKNWLVFGCGPHVCLGQTYVLITFAALIG

KFALYTDFKHKVTPLSEKIKVFATIFPKDDLWMSFKRRDPITGEVTE

 

>CYP61A1 Kluyveromyces lactis

80% to CYP61A1   Saccharomyces kluyveri

KLLA0D11242g

MDNIVVSQTSNATQVASEASVKLFSLANAQQLVETVQQMSYFKLFCTLVAIVIVWDQVSYQIQKGNIAGPKWKVWPVIGP

FLESFDPKFEEYLAKWNSGPLSCVSIFHKFVVIASTRDLARKIFQSPQYVKPCVVDVAVKILRPSNWVFLDGKEHVDYRK

SLNGLFTKQALAKYMPSQELLMDKYIEKFIELSKENKYEARVFFHDMREIMCALSLKAFCGDYITEDQIRKVADDYYLVT

AALELVNFPIIIPYTKTWYGKKTADMTMKIFEQCAQMAKDHIAAGGESTCVLDAWCSLMHEAKNKDDADSKLYHREFTNR

EMSEAIFTFLFASQDASSSLACWIFQIIADRPDVMANIREEQLRVRNNDPNVKLSMDLIDEMKYTNMVVKETLRYRPPVI

MVPYYVKKSFPVVPTYSAPKGSMLIPTLYPALHDPEVYEDPDEFIPERWVEGSAANQAKKNWLVFGCGPHVCLGQTYVMQ

TFTGLIGKFAMFSDWEHKVTPLSEKIKVFATIFPKDDLLLSFKKRDPLTGEVEL

 

>CYP61A1 Uncinocarpus reesii

75% to CYP61A1    Aspergillus fumigatus

UREG_06546.1

MVNAHANMASPSADASLASMSSDPSSLLMSIYNSFTGWSALLALFMLLVAYDQFKYIWLKGSIVGPRFKIPFMGPFLESV

NPKFTEYKAKWASGDLSCVSVFHKFVVIASTRDMARKVFNSPAYVKPCVVDSAHKLLGKDNWVFLDGKAHVEYRKGLNGL

FTRSALTTYMPQLDEVYDKYFKQFLEESKQNDFKPLPWMPYLRELMCALSCRTFVGHYMTDEAVKKIADDYYLITAALEL

VNFPIILPFTKSWYGKKAADMVLEEFSKCAAKSKVRMAAGGEANCIMDGWVKQMLDSAEYREKIAKGIEVDDSEKPKHLL

RDFTDFEIAQTVFTFLFASQDATSSASTWLFQLMADRPDILDKVREENLAVRNGDRNGKISMDILDKMQYTRAVVRETLR

YRPPVIMVPYMTKKDFPITPNYTLPKGCMIVPSVWPAAHDPEAYPNPETFDPDRWITGDAEKAAKNFLIFGTGPHYCLGQ

TYAQLNLMAMIGKASLLMDWEHHATPISEDIKVFATIFPQDDCPLVFRPRA

 

>CYP61A1 Histoplasma capsulatum G217B

77% to CYP61A1   Uncinocarpus reesii

ABBT01000205.1 Ajellomyces capsulatus G217B 1837477-1839379 (+)

ABBT01000210.1 Ajellomyces capsulatus G217B 225529- 227431 (+)

Two identical sequences. Note 51F2 also has two identical sequences

One of them is also on ABBT01000205.1 and the other is on ABBT01000207.1

HCB06044.1 identical to  HCB06499.1

MDTLNGSFASPVADVGLASSVARDPAGLLMSIYNGMTLWTTIFTTLLLLVTYDQVKYIWLKGSIEGPRFKVPFMGPFLQS

VNPKFPEYQAKWASGELSCVSVFHKFVVIASTRDMSRKVFNSPTYVKPCVVDVAHKLLGKDNWVFLDGKEHVEFRKGLNG

LFTRAALTNYMPQLEDVYDRFYNMFVQRSEELNMKPEPWMPHFRELMTALSCRTFVGHYMSDEAVKHVADDYYLITAALE

LVNFPIIIPFTKTWYGKRSSDMVLREFTKCAAKSKVRMAKGEKVTCIMDSWVKNMLDSAAYREKIAKGLPVEDSEKPAHI

LRDFTDYEIAQTIFTFLFASQDATSSASTWLFQLLSDRPEILDKVREENLRIRGGDRNVPISMELLDQMDYTRAVVRETL

RYRPPVIMVPYMVKKDFPVTDTYTLPKGSMIIPSVWPSTHDPEAYPDPETFNPERWLNGEADKAAKNFLVFGTGPHYCLG

QTYAQLNLMAMIGKASMLLDWEHHTTPTSEDIRVFATIFPEDDCPLVFRRRPE

 

>CYP61A1 Coccidioides immitis

90% to CYP61A1   Uncinocarpus reesii

CIMG_05009.2

MASVAGNMASPSADASLASMASDPSSFVMGIYSRFTGWSALLALFMFLVAYDQIKYIWLKGSIVGPRFKIPFMGPFLESV

NPKFTEYKAKWASGELSCVSVFHKFVVIASTRDMARKVFNSPAYVKPCVVDSAHKLLGKTNWVFLDGKEHVEYRKGLNGL

FTRSALSVYMPQLDDVYEKYFKLFLEESKKNNFKPIPWMPYLRELMCALSCRTFVGHYMTEAAVKKIADDYYLITAALEL

VNFPIILPFTKSWYGKKAADMVLDEFSKCAAKSKVRMAAGGEVTCIMDAWIKQMLDSAKYREKIAKGIQVDDSEKPSHIL

RDFSDYEIAQTVFTFLFASQDATSSASTWLFQLMANRPDVLDKVREENLAVRNGDRNGKITMDLLDQLQYTRAVVRETLR

YRPPVIMVPYMTKKDFPITPNYTLPKGCMIVPSVWPATHDPDAYPNPETFDPDRWISGDADKAAKNFLVFGTGPHYCLGQ

TYAQLNLMAMIGKASMMMDWEHHATPISEDIKVFATIFPQREPIRDIPTFVALL

 

>CYP61A1 Mgr052 Mycosphaerella graminicola

67% to CYP61A1 A. oryzae

MASVMQALNASSTDAPNPLYVDIAVASPGLVNKIFDSATPVNVFGAFLALFVAAVIYDQCSYWTHKGTLAGDAWKIPFVG

PFLESVNPQFSKYHQKWLSGALSCVSVFHKFVVIAATRDMARKVLNSPAYVKPCVVDVAHKLLRPENWVFLDGKEHVEYR

KGLNGLFTRTALETYLPGQEEVYNEYFEEFLRESQAAGKPKAWVYYLRELMCAVSCRTFVGHYMSREGVKKIADDYYNIT

AALELVNFPIILPFTKTWYGKKASDKVLEAFANCAAKARIRMKQKGAQPECIMDRWLMQMIESDRYRERIANGEKVPQEE

KPAMLLRNFSDLEISMTVFTFLFASQDATSSAASWMLQLVADRPEVLQKVREEGHRLRPDPNHPVSLETLEKMEYTRAVV

KETLRYRPPVLMVPYLVKKDFPIPEANYVAKKGTMIIPSTWLSLHDPEAYENPDEFAPERWTVGNAEEQGKNWLVFGTGP

HYCLGQTYAVLNLMLMLHKLSKEYEWEHQITDKSEDIRVFATIFPMDDLLLKFKRRDPAMVAAGA

 

>CYP61A1 Mycosphaerella fijiensis

estExt_Genewise1.C_20563

80% to CYP61A1 Mycosphaerella graminicola

MASVMETFNASATHHPNTIHATIDSLQTGFVSKAFDNFTPVSWFGVFLTIFLAAIVYDQLSYWQHKGPLAGDAWKIPFVG

PFLESVNPKFSAYHTKWLSGPLSCVSVFHKFVVIAATRDMARKVLNSPMYVKPCVVDVAHKLLRPENWVFLDGKEHVEYR

KGLNGLFTRQALENYLPGQEEVYNEYFEDFLRESEAAGEPKPWVYQLREIMCAVSCRTFVGHYMSREQVKRIADDYYLIT

AALELVNFPIILPFTKTWYGKKCADKVLEAFSNCAAKSKVRMRQKGAQPECIMDRWVMQMIASERYRERIAKGEKVPDEE

KPPMLLRNFTDLEISMTVFTFLFASQDATSSACSWMLQVVADRPDVMQKVREEGDRLHPNPNEPISLEILESMQYTRAVV

KETLRYRPPVLMVPYLVKKDFPIPEANYVAKKGSMIVPSTWLSLHDPDVYEQPDEFVPERWTIGDAEEKGNKNWLVFGTG

PHYCLGQTYATLNLMLMLHKFSASYDWKHTKTPTSEEIKVFATIFPMDDLLLTIRRREGVATA*

 

>CYP61A1 Fusarium graminearum FG01959.1 AACM01000104 FGcontig1.104_scaffold1

MEAVNSTTSGFSSVLAGTKYANVNIPPQIDYVXXXXIEAVSNAGVWTWVFTLVA

LCIAYDQIAYIVRKGPIVGPAMKIPFIGPFLDSMDPRFDGYHAKWSSGPL

SCVSIFHKFVVIASTRDMARKVFNSPAYVKPTVVDVAPKLLGHDNWVFLD

GKAHVDFRKGLNGLFTRKALELYLPGQEEAYNTYFKHFLKMTKDAGGKPV

PFMHEFREVMCAVSCRTFVGHYISDEAVTKIAEDYYLITAALELVNLPVI

LPYTKSWYGKKAADMVLAEFSKCAAKSKVRMAAGGEVTCIMDAWILSMIQ

SERWRKAEEKGEPHNVEKPSPLLRMFNDYEISQTIFTFLFASQDATSSAA

TWLFQVTAQRPDVLDRVREENIKIRNGDPNAPLTMDQLESLTYTRAVVRE

LLRWRPPVIMVPYVTKKAFPLTDDYTVPKGSMLIPTTFMALHDPEVYDNP

SHFDPERYYSGDAEEKGSKNYLVFGTGPHYCLGQVYAQLNLALMIGKASV

MLDWKHHATPKSEEIKVFATIFPMDDCPLTFEERKW*

 

>CYP61A1 AAIM02000091.1 Gibberella moniliformis 7600

95% TO FUSARIUM GRAMINEARUM 61A1

99% to 61A1 F. oxysporum

also Fusarium verticillioides FVEG_07284

        MEAVNATSSGFSSVLAGTKYVNVALPPQVEYVIEAVSNAGVWTWVFTFIALCVAYDQ (1)

350695  IAYIIRKGPIEGPAMKLPFIGPFLDSMDPRFDGYHAKWSSGPLSCVSIFHK (2) 350847

350776  FVVIASTRDMARKVFNSPAYVKPTVVDVAPKLLGHDNWVFLDGKAHVDF  351054

351055  RKGLNGLFTRKALESYLPGQEEAYNTYFKHFLKMTKDAGGKPVPFMHEFREVMCAVSCRT  351234

351235  FVGHYISDEAVTKIAEDYYLITAALELVNLPVILPYTKSWYGKKAADMVLAEFSKCAA  351408

351409  KSKVRMAAGGEVTCIMDAWVLSMIQSERWREAEEKGEGHTVEKPAPLLRMFNDYEISQTI  351588

351589  FTFLFASQDATSSAATWLFQVTAQRPDVLDRVREENIKVRNGDPNAPITMDQLESLTYTR  351768

351769  AVVRELLRWRPPVIMVPYVTKKAFPLTENYTVPK (1)

        GSMLI  351948

351949  PTTFMALHDPEVYDNPSHFDPERYYSGDAEEKGSKNYLVFGTGPHYCLGQVYAQLNLALM  352128

352129  IGKASVMLDWKHHATPKSEEIKVFATIFPM  352218

        DDCPLTFEERKW*

 

>CYP61A1 Fusarium oxysporum

99% to 61A1 Gibberella moniliformis

FOXG_04166

MEAVNATSSGFSSVLAGTKYANVALPPQVEYVIEAVSNAGVWTWVFTFIALCVAYDQIAYIIRKGPIEGPAMKLPFIGPF

LDSMDPRFDGYHAKWSSGPLSCVSIFHKFVVIASTRDMARKVFNSPAYVKPTVVDVAPKLLGHDNWVFLDGKAHVDFRKG

LNGLFTRKALESYLPGQEEAYNTYFKHFLKMTKDAGGKPVPFMHEFREVMCAVSCRTFVGHYISDEAVTKIAEDYYLITA

ALELVNLPVILPYTKSWYGKKAADMVLAEFSKCAAKSKVRMAAGGEVTCIMDAWVLSMIQSERWREAEEKGEGHTVEKPT

PLLRMFNDYEISQTIFTFLFASQDATSSAATWLFQVTAQRPDVLDRVREENIKVRNGDPNAPITMDQLESLTYTRAVVRE

LLRWRPPVIMVPYVTKKAFPLTENYTVPKGSMLIPTTFMALHDPEVYDNPSHFDPERYYSGDAEEKGSKNYLVFGTGPHY

CLGQVYAQLNLALMIGKASVMLDWKHHATPKSEEIKVFATIFPMDDCPLTFEERKW*

 

>CYP61A1 Magnaporthe grisea MG06047.4  51% to 61A1 S.c. 72% to 61A N.c.

AACU01000857 cont2.1129

MASVAVGVANATAAFTSPLASYVPSNVALPPQLEYVLGNVIETVSNAGFW

TILGTVVLMLAIYDQFSYQRSKGNIVGPRFKEPFIGPFLQSMNPKWEEYY

GKWTSGPLSCVSVFHKYAYTFPHSKTVTELLNMPCVVDVAPKLLGHDNWV

FLDGKAHVDFRKGLNGLFTRKALECYLPGQEDVYNRYFKKMVQITKDAGG

KPVPFMVEFREIITAVSCRTFVGHYMSDETVRRIAVDYFYITEALELVNF

PIILPFTKTWYGKKAADMVLAEFSKCAAKSKARMNADGEVTCIMDAWIQQ

MVLSQRWRDAEAAGTITDDMEKPNPLLRDFTDYEIAQTLFTFLFASQDAT

SSAATYMFQIMAQHPEVLDRVREENYNVRNGDINARVSLEQLESMKYTRA

VVKELLRYRPPVIMVPYVTKKAFPITPEYTAPKGSMLIPTTYMALRDPEV

YDRPDEFDPERYYTGDAEEKGQKNFLVFGTGPHYCLGQHYAQLNLALFVG

MASLQLDWKHHATPLSEEIQVFATIFPKDHCPLTFDWRKWN*

 

>CYP61A1 Nectria haematococca

fgenesh1_pg.scaffold_12000391

Necha1/scaffold_12:1044679-1046547

88% to CYP61A1 Fusarium graminearum

53% to CYP61A1 S. saccharomyces

This gene model seems correct DRN 2/6/06

MASVNSTGGFTSVLANSKYANVGIPPQLDFVIDAVSNAGIWTWIATIVAVCVVYDQ (1)

ISYIMQKGSLVGPSWKMPFIGPFLDSMDPRFDGYHAKWESGPLSCVSIFHK (2)

FVVIASTRDMARKVFNSPGFVKPTVVDVAPKLLGHDNWVFLDGKAHVEFRKGLNGLFTRKALESYLPGQE

ESYNTYFKHFLQMTQEAGGKPVPFMHEFREVMCAVSCRTFVGHYISDEAVKKIAHDYYLITAALELVNLP

VILPWTKSWYGKKAADMVLHEFSKCAAKSKVRMAAGGEVTCIMDAWVLAMVQSQRWREADEKGEADGMEK

PTPLLRMFNDYEISQTIFTFLFASQDATSSAATWLFQVTAQRPDVLDRVREENIKVRNGDPNAAVTMDQL

ESLTYTRAVVRELLRYRPPVIMVPYVTKKAFPITDSYTVPK (1)

GSMLIPTTYMALHDPDVYENPDYFDPDRYYKGDAEEKGAKNYLVFGVGPHYCLGQVYAQLNL

ALMIGKASVMLDWKHHATPKSEEIKVFATIFPM  (0)

DDCPLTFEERKW*

 

>CYP61A1 Aspergillus nidulans AN4042.1 66% to 61A1 51 clan revised

MAEINGSFVSPAADATVFPQVFQPAGLIADFLNGLTLWKTLATLFALAVV

YDQFRYIYLKGAIVGPAWKLPFMGPFLQSVNPKFHEYKAKWDSGELSCVS

VFHKFVVIASTRDMSRKIFNSPTYVKPCVVDAAHKLLGKTNWVFLDGKEH

VDFRKGLNNLFTRQALSCYLPRMEEVYNDYYARFLKKSKNNNYKPTPWMP

EFRDLMCAVSCRTFVGHYISDEAIDKISVDYYNITAALELVNFPIILPFT

KTWYGKKAADMVLDEFAKCAAKSRARMAAGGEISCIMDAWIKAQLDSAKY

REKIAKGIEVDSSEKPPQVLRDFTDYEVSQTIFTFLFASQDATSSACTWL

FQLMADRPEILDKVREENLRLRNGDVNAPLTMDLLDSMTYTRAVVKETLR

YRPPVIMVPYIAKKDFPITDKITVAKGSMIIPSVYPATRDEEAYPNADSF

DPDRWITGTAEQHPKNFLIFGTGPHYCLGQTYAVLNLMAMIGKASMEMDW

VHTPTPQSEEIKVFATIFP (0)

DDCLLTFRPRA

 

>CYP61A1 Aspergillus oryzae

AP007155.1

MANVTGSFVSPSADATVVPQLFQPSGLLGSLLGDFNVWKGLLTLFIAAVIYDQ (0?)

FRYFYQKGSIVGPRWKLPFMGPFLQSVNPKFHEYKAKWDSGELSCVSVFHK (2?)

FVVIASTRDMSRKIFNSPAYVKPCVVDSAHKLLGEDNWVFLDGKDHVEFRKGLNGL

FTRSALSCYLPRQEETFNQYFKHFLEKSKANGYKPTPWMPEFRELMTAISCRTFVGHY

MTDEVIQKINDDYYLITAALELVNFPIILPFTKTWYGKKAADMVMEEFAKCAAKSKAR

MAAGGEVSCIMDAWVKAQQVSAKYREDVAKGIPAEKPPQLLRDFTDEEIAKTVFTFLF

ASQDATSAASTWLFQLMADRPEVLEKVREENVRLRNGDINAPITMELLDQMEYTRAVV

KETLRYRPPVIMVPYLVKKDFPITEKITVLKGSMIIPSVWPATHDEEAYPNPDTFDPD

RWITGTAEQNPKNWLVFGTGPHYCLGQTYAQLNLMAMIGKASMEMTWEHTTTPKSEDI

KVFATIFPQ (0)

DDCLLTFRPRA

 

>CYP61A1 Aspergillus flavus

100% to CYP61A1 Aspergillus oryzae

AFL2G_02074

MANVTGSFVSPSADATVVPQLFQPSGLLGSLLGDFNVWKGLLTLFIAAVIYDQFRYFYQKGSIVGPRWKLPFMGPFLQSV

NPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDSAHKLLGEDNWVFLDGKDHVEFRKGLNGL

FTRSALSCYLPRQEETFNQYFKHFLEKSKANGYKPTPWMPEFRELMTAISCRTFVGHYMTDEVIQKINDDYYLITAALEL

VNFPIILPFTKTWYGKKAADMVMEEFAKCAAKSKARMAAGGEVSCIMDAWVKAQQVSAKYREDVAKGIPAEKPPQLLRDF

TDEEIAKTVFTFLFASQDATSAASTWLFQLMADRPEVLEKVREENVRLRNGDINAPITMELLDQMEYTRAVVKETLRYRP

PVIMVPYLVKKDFPITEKITVLKGSMIIPSVWPATHDEEAYPNPDTFDPDRWITGTAEQNPKNWLVFGTGPHYCLGQTYA

QLNLMAMIGKASMEMTWEHTTTPKSEDIKVFATIFPQDDCLLTFRPRA

 

>CYP61A1  Aspergillus fumigatus Af293

         GenEMBL XP_750145.1, NZ_AAHF01000007 

         cytochrome P450 sterol C-22 desaturase

         81% to 61A1 Aspergillus oryzae

MANVNGSFVSPSADATISPQLFYNVDSLSAVLNGFTFWKALATLFFAAVIYDQLRYFYLKGSLVGPTFKL

PFMGPFLQSVNPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDIAHKLLGPD

NWVFLDGKEHVEFRKGLNGLFTRSALSSYLPVMEECYNKYYKYFLEKSKANDYKPEPWMPEFRELMCAVS

CRTFVGHYMTDAAIKKIADDYYMITAALELVNFPFILPFTKAWYGKKASDMVLEEFSNCAAKSKAHMAAG

GEITCIMDAWVKAQQDSAKYNEKIAKGLPVEDSEKPSHLLREFTDYEIAQTVFTLLFASQDATSAACTWL

FQLVADRPDVLEKIREENLRVRNGNINAPLTMDLLDEMKYTRAVVRETLRYRPPVIMVPYLVKKDFPITD

SITVSKGSMIIPSVWPATHDPEAYPNPDSFDPDRWITGDAEKQAKNFLVFGTGPHYCLGQTYAQLNLIAM

IGKASLEMDWEHAPTPKSEDIKVFATIFPE (0)

DDCHLTFRPRA*

 

>CYP61A1 Neosartorya fischeri

99% to CYP61A1 Aspergillus fumigatus = ortholog

NFIA_020740

MANVNGSFVSPSADATISPQLFYNVDSLSAVLNGFTFWKALATLFFAAVIYDQLRYFYLKGSLVGPTFKLPFMGPFLQSV

NPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDIAHKLLGPDNWVFLDGKEHVEFRKGLNGL

FTRSALSSYLPVMEECYNKYYKHFLEKSKANDYKPEPWMPEFRELMCAVSCRTFVGHYMTDAAIKKIADDYYMITAALEL

VNFPFILPFTKAWYGKKASDMVLEEFSNCAAKSKAHMAAGGEVTCIMDAWVKAQQDSAKYNEKIAKGLPVEDSEKPSHLL

REFTDYEIAQTVFTLLFASQDATSAACTWLFQLVADRPDVLEKIREENLRVRNGNINAPLTMDLVDEMKYTRAVVRETLR

YRPPVIMVPYLVKKDFPITDSITVSKGSMIIPSVWPATHDPEAYPNPDSFDPDRWITGDAEKQAKNFLVFGTGPHYCLGQ

TYAQLNLIAMIGKASLEMDWEHAPTPQSEDIKVFATIFPEDDCHLTFRPRA*

 

>CYP61A1 Aspergillus clavatus NRRL

NZ_AAKD03000006.1

87% TO ASPERGILLUS FUMIGATUS CYP61A1, 52% TO CYP61A6 A. clavatus

ACLA_030640

468671  MANINGSFVSPSADATLSPQFFQSAEFLPAILNGFTIWKALVTLFIAAVIYDQ (1)  468513

        LRYFYLKGSLVGPTFKLPFMGPFLQSVNPK 468315

468314  FHEYKAKWDSGELSCVSVFHK (2)

        FVVIASTR  468135

468134  DMSRKIFNSPAYVKPCVVDVAHKLLGADNWVFLDGKDHVEFRKGLNGLFTRSALSCYLPE  467955

467954  MEECYNQYYKRFLKKSKANQYKPEPWMPEFRELMCAVSCRTFVGHYITDEAVQKIADDYY  467775

467774  MITAALELVNFPFILPFTKAWYGKKASDMVLQEFSNCAAKSKARMAAGGEISCIMDAWVK  467595

467594  SQLDSAKYREKIANGVPPEEAGKPSHLLRDFTDYEIAQTIFTFLFASQDATSAACTWLFQ  467415

467414  LMADRPEILDKVREENVRVRNGDITAPLSMDLLDQMTYTRAVVKETLRYRPPVIMVPYLV  467235

467234  KKDFPITDSVTVAKGSMIIPSVWPATHDPEAYPNPDSFDPDRWITGDADKHPKNWLVFGT  467055

467054  GPHYCLGQTYAQLNLMAMIGKASMEMDWVHTPTPESEDIKVFATIFPQ  466911

        DDCHLTFRPRA*

 

>CYP61A1 Aspergillus niger

estExt_GeneWisePlus.C_12230|Aspni1

84% to CYP61A1

MASVNGSFVSPSADAVVSPRLFEPTGLVASVLEGFTVWKALLTLFVAAVIYDQFRYLWLKGSIVGPAWKLPFMGPFLQSV

NPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPTYVKPCVVDVAHKLLGADNWVFLDGKDHVEFRKGLNGL

FTRQALSFYLPRMEEVYNEYYARFLRISKENNFKPTPWMPEFRELMCAVSCRTFVGHYITEEAIQKIADDYYMITAALEL

VNFPFILPYTKAWYGKKAADMVLAEFSNCAAKSKARMAAGGEISCIMDAWVKAQQDSAKYREKIAQGIAVEASDKPAQVL

RDFTDYEIAQTVFTFLFASQDATSAASTWLFQLMADRPDVLDKVREENLSVRNGDIKAPLTMELLDQLPYTRAVVKETLR

YRPPVIMVPYLVKKDFPITENVTVSKGSMIIPSVWPACHDEEAYPNADSFDPDRWITGTAEQQTKNWLVFGTGPHYCLGQ

TYAQLNLMAMIGKASMEMDWEHTPTPESEDIKVFATIFPQDDCLLAFRPRP*

 

>CYP61A1 Aspergillus terreus

87% TO CYP61A1 Aspergillus niger

70% to CYP61A1 Aspergillus terreus

55% to CYP61A6 Aspergillus terreus

ATEG_03815.1

MATVNGSFVSPSADATIVPQLFAPSGLIGSVLEGFTVWKALLTLFLAAVIYDQLRYFWLKGSIVGPAMKLPFMGPFLQSV

NPKFHEYKAKWDSGELSCVSVFHKFVVIASTRDMSRKIFNSPAYVKPCVVDAAHKLLGADNWVFLDGRDHVDFRKGLNGL

FTRQALSCYLPRMEEVYNDYYARFLKKSKDVNYKPVPWMPEFRELMCAVSCRTFVGHYITDEAVQKIADDYYLITAALEL

VNFPIILPYTKTWYGKKAADMVLEEFSKCAAKSKARMAAGGDISCIMDAWVKQQLDSARYRENVAKGVPVDENEKPAQVL

RDFTDYEIAQTIFTFLFASQDATSAACTWLFQLMADRPEVLDKVREENLRVRNGDVNAPLTMDVLESLTYTRAVVKETLR

YRPPVIMVPYLVKKDFPVTDKITVSKGSMIIPSVWPATHDEEAYPNADSFDPDRWITGTAEQQSKNWLVFGTGPHYCLGQ

NYATLNLMAMIGKASMEMNWEHTPTPQSEDIKVFATIFPQDDCLLTFRPRA

 

>CYP61A1 Ustilago maydis

GenEMBL XM_397965.1 in the 61 clan 49% to CYP61 N.crassa

MASILGVGQQGLNFSRSSAPSSPLIPRDSALGKVFSIVDLTRLPSLQEADRTTVVFTILAVVATLLLAEQ

YTYQKKKAGLPGPKWTIPVIGKFADSLHPTLEKYQEGWNSGPLSVASVFNIFIVIASSNEYTRKILNSPT

YAEPCLVASAKKVLCPDNWVFLNGKTHVDYRRGLNTLFTRQALGIYLGIQETIYKRYFEEWLADTNPNPQ

PYMMKFRDLNMETSLRVFCGNYIPDEGAKQISDNYWLITVALELVNFPFAFPGTKVYNAIKARKTAMKWF

ELTARESKKRMAAGEEVTCLTDAWIKAMVDARLQRDNADLEAETRRVLLRDFSDREIGMVLLSFLFASQD

AMSSGLTYLLQHVADRPDVLRKVREEQYRVRGDDINAALTYNAIEQMEYTKAVVKESLRIKPPVIMVPYL

TQKDFPIDKNYTVPKGSMVIPSFWNSLHDEKVYPQPDEFKPERWLDEADPANKSPKNYLVFGSGPHYCIG

QQYANMHLTAVLGTASVLMNWEHEVTPLSEKVEVIATIFPKDGARIKFSRRAAPAPGTAPEVAARA

 

>CYP61A1 Sporobolomyces roseus

57% to CYP61A1   Ustilago maydis, estExt_fgenesh1_pm.C_40050

MASTPHLSAQVAAASESPLFGKLAPSFDKLNIEWSTQTIATSVLAVIVTLLIAEQTLWRYRKSTLPGHSWQIPVIGQFAE

SLNPTMEAYKRSWSTPLASVSFIVIASTTEYARKILNSSTLAEPCLVRSAKQILLPENWVFLNGKVHNEYRKGLNCLFTP

QALEIYLKKQDQIYRNHFRAWLSDPNPNAQPYQLKFRDLNMETSLKVFCGDYISDKNAKEVSEKYWQITQALELVNFPFA

FPGTKVWSACRAREVTVKYLAQAAAASKKAVAAGKEPDCLLDEWVEEIHAGKVRKYEDKEMALVLLSFIFASQDAMSSSI

TFAFQLLADHPEVLAKVREEQAAVRHGDYETPMTLALLEQMPYTNAVTKEVLRYRPPVIMVPYMTKKPFPINEEYTVPKG

TMLIPSFWNSLHDEKVYPDAEEFIPERFMPGGVNDNDKDAKNWLVFGAGAHRCIGQNYVYMHMTAVIGSAAMLMNWEHAK

TPESEEIQIIATLFPKDGCHLKFTPREEAATAL

 

>CYP61A1 Malassezia globosa

74% to CYP61A1   Ustilago maydis, MGL_0310

MSHGQSQQVWDTAMTGNETIPQHTLNTATAQEGFTAVQVAFAVLTVIVSLLALEQMVYRMKKGGLPGSKWTIPIIGKFKD

SLNPTLEKYQESWNSGALSVASVFNIFIVIASSTDYTRKILNSPTYAEPCLVASAKKVLCHDNWVFLNGKTHVDYRKGLN

TLFTSRALSIYLNIQENIYKKHFAKWVALGSKDAEAFMLPLRNLNLETSLRVFCGNYISDEGAQQISDEYWLITMALELV

NFPFAFPGTKVYRAIQARKNAMKWFEHTAAESKKRMALGEEVTCLTDAWVKAMLDAREDRQNDDLGAEQRRILVRDFSDR

EIGMVLLSFLFASQDAMSSGLTYLFQHLADHPDVLRKVREEQYAIRNNDIDAPLTIDMIEKMTYTRAVVKESLRLKPPVI

MVPYMARQPFPISETYTAPKGSMVIPSFWNSLHDPTAYPSPDEFRPERWLEGAESPAAKHPKNYLVFGSGPHNCIGKEYA

MQHLIAVMGDASVLLNWEHKRTELSEKVMVIATIYPKDGAYLKFTQRPAPPMDAPAAVAAAM

 

>CYP61A1 Chaetomium globosum CBS 148.51

NZ_AAFU01000895.1 AAFU01000895.1

84% TO N. CRASSA 61A1

      MAANATTSPLATIKYGAASVAPQLEYVIDYVSNASTLSILATILATLVVYDQ (1)

6856  FRYILNKGSIAGPAWKMPFIGPFLQSMNPKFEEYYAKWLSGPLSCVSVFHK (2)  7008

7135  FVVIASTRDMARKVFNSPAFVKPCVVDVAHKLLGADNWVFLDGKAHVEF  7215

7216  RKGLNGLFTRRALEIYLPGQEEVYNRYFKEFVATTKQAGGEPVPFMTHFREVITAVSCRT  7395

7396  FVGHYITDEAVKKIADDYYLITAALELVNFPIILPYTKTWYGKKAADMVLAEFAKCAA  7569

7570  KSKARMAAGGEPNCIMDAWIIQMIQSQRWRDAQAKGDTEGVEKPTHILRDFTDYEISQTV  7749

7750  FTFLFASQDATSSAATWLFQIMAQRPDVLDRVREENLNVRNGDINAAVNMDQLESMTYTR  7929

7930  AVVRELLRYRPPVLMVPYVVKKPFPITETYTAPK (1)

      GSMVIPT  8109

8110  TYMALRDPEVYDRPDEFDPERYYTGDAEVKGMKNYLVFGTGPHYCLGQQYAQLNLALMVG  8289

8290  KASLLLDWKHHATPKSEEIKVFATIFPM  8373

      DDCPLTFEDRKW*

 

>CYP61A1 ug.78.18.1(CYP61) Phanerochaete chrysosporium

MASSQAAFPSTLSDSSRHSTDSPAFIGLLPTGSWFYTTAAILLSLLVIEQSVYRYKKRHLPGDKWTIPLIGKFADSMKPTMEGYMKQWNSGALSAISVFNVRFIVMASTTEYARKILNSPTYAEPCLVHSAKQIILPDNWVFLTGKEHVEYRRGLNLLFTRKALGYVLQYCLVLYAMLTHRRSLYLGIQDVITRKHFAKWLADAAKDPSAKPIMMTARELNMETSLRVFCGNHIPEHGAKEISDKYWMITVALELVNFPLAIPGTKVYNAIQARKAAMKWLELAARKSKESVAAGNPPECMLEEWVTILNDPAYKGRREFSDHEMAMVVFSFLFASQDAMSSGLIYGFQHLADHPEVLAKVREEQERVRGGDYEKPLTLEMMDEMPYLRAMVKETLRVKPPVTMVPYKTTKAFPISQDYTVPSGSMVIPSFYNSLHDPAVFPDPDRFMPERWLDPNGSANTNPRNYLVFGSGPHKCIGLEYAMMNIALVLANAAVLMNWEHELTPQSDKVQIIATLFPQDGCKLKFSPRQHA

 

>CYP61A1

Cryptococcus neoformans var. neoformans B-3501A chromosome 6

61% to CYP61A1   Ustilago maydis

AAEY01000030

CNBF1100"

ESTs gb|CF191501.1|CF191501,gb|CF188802.1|CF188802, gb|CF194425.1|CF194425

EAL20298.1

MESHTVLRPTAIPDLAAIKTWGLEGLTKAKFSFDTKTTTATILT

LILSLLVLEQLVYRAKKAHLPGAKWTIPVIGKFADSLNPTLANYKAQWNSGPLSAVSV

FNIFIVIGSSNEMARKILNSPNHAEPCLVASAKKVLLPENWVFLHGKVHADYRKALNV

LFTKQALSIYLPIQEKIYRSYFNKWMSDPAPAQQYMMKMRDLNMDTSLSVFIGPYLTE

AQKQEINDKYWLITISLELVNFPLAIPGTKVYNAIQARKIVMKYLSAASAASKIRMED

DDAEPECLLDHWVRAMILARRAKDDGEQTRLLSREYSDHEIAMVLLSFLFASQDAMSS

ALVYTFQLTADHPEVLEKVREEQYRVRGNDLERPLTLDMLDDMVYTRATIKEVLRFRP

PVIMVPYMTTKPFPVSPEYTAPKNSMIIPAFWNSLHDETCYPEPDRFLPERWLPQADG

SAPIADSKPQNYLVWGSGPHKCIGGQYASMHLAATLGTASVLMDWKHERTELSDEVQV

IAAIFPKDHCLLKFTPRAPPS

 

>CYP61A1 P450-10_var1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

65% to P450-8 = CYP61 C-22 sterol desaturase

53% to CYP61A3   S. pombe

RO3G_04913.1

MSTSPSLFNPRSIESAKNAINSFVKSALNTQPSTTVLVCSALVGLFAYEQYVYLKKKKSLPGPFFKIPIIGAFMDSLYPTFEGYMSKWKSGELSCVSVFDRFVVIASTCELSRKILNAPAYAEPAVVDSMRHILCADNWVFLSGKAHVDYRKGLNVLFT RKALGIYIPIQERVYQKHFDEWLSLDGKSEQYQLRFREINMEASLRVFLGDYMSDQVAQQISEEYFNITAALELVNFPIPLPGTKVYKAIQSRKFIVKHFIECIKDCRIRMAQGGEVKSMMDAWISSMAEYEVECEKAGKPAPRKFDDREVALTILTFLFASQDATSSALTWAFQLLADHPDVLEKVYQEQLRLRKDNLQQELSLELMEESTYLRQVVKEILRLRPPVLMVPYQTVREWPITPSYTIPKKAMVIPTTYPALHDPVAYPNPDAFDPDRWGPEGIAEKHPKNFMVFGNGPHHCLGKEYAVMHLMAVIAQATLQMEWTHTRTEKSDDISIFATIYPQDGCIMSFKPRTITN

 

>cyp61A1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

81% to CYP61A8 Rhizopus oryzae, 80% to CYP61A1 Rhizopus oryzae

e_gw1.3.127.1

MKTAFSQPNISTTAVICSAVVGLFAYEQYVYLNKKKGLPGPQFKIPIIGAFTDSLYPTFEGYMSKWKSGELSCVSVFDRF

VVIASTCDLSRKILNAPVYAEPAVVDSMKHILCADNWVFLSGKSHVDYRKGLNVLFTRKALGIYIPVQDKVYKKHFAEWL

SLNGEKGQYQLRFRELNMESSLRVFLGDHMSDEVASKISQEYFNITAALELVNFPIPLPGTKVYKAVQSRKYIVNQFVEC

IKDCRTRMANGGETKSMMDAWVRSMLDTEAELAAAGKPAPRKFDNREIALTILTFLFASQDATSSAMTWAFQLTADHPDV

LHKIRSEQMKLREENPDQELTLELMEKSVYLRQVIKEVLRIRPPVLMVPYQTVREWPITPNYTVPKGSMVIPTTYPALHD

PVAYPNPDNFDPDRWGTEGTAEKYPKNFMVFGNGPHHCLGKEYAVMHLMATLAEACMVLDWTHYRTDKSDDISIFATIYP

QDGCIMSFEPRKSPASIST*

 

>CYP61A2 C.albicans AL033396 comp(39932..41485) gene="Ca35A5.10c" 68.9% identical to 61A1

orf19_5178

MNSTEVDNLPFQQQLTSFVELAVAKATGSPITTLFTIIFLILSY

DQLSYQINKGSIAGPRFKFYPIIGPFLESLDPKFEEYKAKWDSGELSCVSIFHKFVVI

ASSRDLARKILSSPKYVKPCVVDVAIKILRPTNWVFLDGKQHTDYRRSLNGLFSSKAL

EIYIPVQEKYMDIYLERFCKYDGPREFFPEFRELLCALSLRTFCGDYITEDQIALVAD

NYYRVTAALELVNFPIIIPYTKTWYGKKIADDTMKIFENCAAMAKKHINENNGTPKCV

MDEWIHLMKEAREKHSEDPDSKLLVREFSNREISEAIFTFLFASQDASSSLACWLFQI

VADRPDIVAKIREEQLRVRNNNPDVRLSLDLINEMTYTNNVVKESLRYRPPVLMVPYV

VKKSFPVTESYTAPKGAMIIPTLYPALHDPEVYDEPDSFIPERWENASGDMYKRNWLV

FGTGPHVCLGKNYVLMLFTGMLGKFVMNSDMIHHKTDLSEEIKVFATIFPKDDLILEW

KKRDPLKSL

 

>CYP61A3 S. pombe Z98974 join(10345..10419,10514..12001) gene="SPAC19A8.04" 51% identical to CYP61A1

MEPSDQIIRFNDKFTTISYLPWILIMQKGHIPGPRFKIPFMGSF

LDSMKPTFEKYNAKWQTGPLSCVSVFHKFVVIASERDLARKILNSPSYVQPCVVDAGK

KILKHTNWVFLDGRDHIEYRKGLNGLFTTRALASYLPAQEAVYNKYFKEFLAHSKDDY

AQYMIPFRDINVATSCRTFCGYYISDDAIKHIADEYWKITAAMELVNFPIVLPFTKVW

YGIQSRKVVMRYFMKAAAESRKNMEAGNAPACMMEEWIHEMIETRKYKSENKEGAEKP

SVLIREFSDEEISLTFLSFLFASQDATSSAMTWLFQLLADHPDVLQKVREEQLRIRKG

DIDVPLSLDLMEKMTYTRAVVKECLRLRPPVLMVPYRVKKAFPITPDYTVPKDAMVIP

TLYGALHDSKVYPEPETFNPDRWAPNGLAEQSPKNWMVFGNGPHVCLGQRYAVNHLIA

CIGKASIMLDWKHKRTPDSDTQMIFATTFPQDMCYLKFSPFDASTVDWKNSKEAFSNE

AVSAATVETESA

 

>CYP61A1 Schizosaccharomyces japonicus

80% to S. pombe CYP61A3 (misnamed, should be CYP61A1) SJAG_03249

MTKVSFVVATFSFESLSYQIQKGTIPGPIVKIPFMGSFLDSMKPTFEKYHTKWMSGPLSCVSVFHKFVVIASEREMSRKI

LNSPNYVQPCVVDAGKKILKPNNWVFLDGKAHVEYRKGLNGLFTRRALANYLPAQEAVYNKYFKEFLDYSKDDYKQYMIP

FRDINVAVSCRTFCGSYISDDAITEIADHYWRITAAMELVNFPIVLPFTKIWYGIKARDVVMKFFMNAAAQSRKNMEAGK

PITCMMDEWIHEMIEAREYKEKGAAEGAEKPSILIRDFSDEEISLTFLSFLFASQDATSSAMTWLFQMLADNPEVLRKVR

EEQISIRNGDPKAPITIDLVDKMEYTRWVVKECLRLRPPVLMVPYRVKKGFPIRSDYTIPKDSMVVPTLWCALHDPEVFP

EPDNFIPERWGPEGTAEKSPKSWLVFGTGPHVCLGQRYAVMHLIACIGKASIELDWKHKRTEDSDTQMIFATTFPQDMCY

LKFSPFDSTKLWNELRHKKHEKPEKHEGHDLQREQSINTVASSTTESLAEGLTQTVTEA

 

>CYP61A1 Schizosachharomyces octosporus

SOCG_00808

MNQTESFLSGSQNVVRLMGYEVEYTRWTVCFALLLVCIAYDQIAYQLRKGNLPGPLIKIPFMGSFLDSMKPTFEKYKTKW

DSGPLSCVSVFHKFVVIASERDLARKILNSPSYVQPCVVDAGKKILKHTNWVFLDGRDHVEYRKGLNGLFTTRALGVYLP

AQEEVYNKYFKEFLDHSQGKYTEFMIPFRDINVATSCRTFCGHYISDDAIKHIAEEYWNITAAMELVNFPLVLPFTKVWY

GIRSRKIVMQYFMKAADASRKSMEAGNAPTCMMEEWIREMIETRKYNNSKGLDGSEKPSVLIRDFSDEEISLTFLSFLFA

SQDATSSAMTWLFQYLADYPEILQKVREEQFRIRDGDPNVPISLELLEKMTYTRMVVTECLRLRPPVLMVPYRVKKSFPI

SPEYTVPKDSMVIPTLYGALHDPKVYPDPESFKPERWEPNGLAQQNPKNWMVFGNGPHVCLGQRYAVNHLIACVGKASLM

LDWSHKRTVDSDTQMIFATTFPQDMCLLKFTPFNPATIKLHLKEDAELQTPTAAGIPSSA

 

>CYP61A4    Botrytis cinerea (a plant-pathogenic fungus infecting over 200

            plant species)

            GenEMBL AL111744 AL111746

            Bitton,F., Levis,C., Fortini,D., Pradier,J.M. and Brygoo,Y.

            58% to S. pombe 61A3, 77% to 61A1 N. crassa

            note: most fungi have only one CYP61 gene

            so this is probably the ortholog of CYP61A1

            but for historical reasons it was named CYP61A4

EVLDKVREFTDFEIAQTVFTFLFASQDATSSAATWLFQVMAQRPEVLDKVREENLRVRGG

DKRVRPNMDMMESMKYTRAVVRELLRWRPPVLMVPYVAKKAFPITPDYTVPKGAMIVPTT

YPALRDPDVYDRPDEFDPERYFSGDAEVKGAKNYLVFGTGAHYCLGQEYAQMNLALMIGK

ASMELDWVHHPTPKSEEIKVFATIFPMDDCPLTFTKRS*

 

>CYP61A5     Neurospora crassa

            Neurospora crassa sequence contig 1.384  (supercontig 48)

            note: most fungi have onlt one CYP61 gene

            so this is probably the ortholog of CYP61A1

            but for historical reasons it was named CYP61A5

            AABX01000229.1 cont3.300 NCU05278.1 (version3)

      MASNATSFTSPLASAKLGTVAVPPQLEYVIDTISQASGWTIVFT

      LLAVLVAWDQ (1) 24057

24127 IKYVLNKGTIAGPSWKIPFIGPFLESLDPKFEEYYAKWLSGPLS

      CVSVFHK (2) 24279

24350 FVVIASTRDMARKVFNSPSYVKPCVVDVAHKLLGADNWVFLDGPA

HVEFRKGLNGLFTRRALEIYLPGQEEVYNRYFKSFLEITKNAGGKPVPWM

PHFREVITAVSCRSFVGHYISDEAVKKIADDYYLITAALELVNFPIIIPY

TKTWYGKKAADMVLAEFAKCAAKSKVRMAAGGEATCIMDAWIKQMIDSKK

WREAVASGNTEGLEKPTHLLRDFTDYEISQTIFTFLFASQDATSSAATWL

FQIMAQRPDVLDRVREENLKVRNGDIHAPINMEQLESMTYTRAVVRELLR

YRPPVLMVPYLVKKAFPITEDYTIPKGSMVVPTTYMALRDPEVYPNPDYF

DPERYYSGDAEAKGAKNYLVFGVGPHYCLGQHYAQLNLALFVGKASLLLD

WKHHPTPLSEEIKVFATIFPK (0) 25597

25660 DDCPLTFEERKW* 25698

 

>CYP61A6 Fusarium graminearum FG03686.1 AACM01000161 FGcontig1.161_scaffold2

MASLLDCGAGLASANASFSTATDLSQENGLFSSWFERCSKTQIAVTIFAV

LIAYDQFMYIWRKGSIAGPAFKIPFMGPFIQALYPKFDAYLAQWASGPLS

CVSVFHKFVVLASDRDIAHKVFKSPTFVKPCIVPMAETLLRPSAWVFLQG

RAHTEYRRGLNGLFTNKAISTYLPAQEKVYDDYFERFVAASEANKSKPMA

FMRLFREINCALSCRTFFGDYISQDAVEKIAEDFYQVTAALELVNVPLSV

YIPFTKCWRGKRTADAVLAEFASCAAACKANMAAGAEPKCIVDQWVLHMM

ESKRYNDRIAAGETGAEKPKNLIREFTDEEIGQTMFTFLFASQDASSSAT

TWLFQILAQRPDVLDRLREENLSVRGGNKETPFELSMLESLPYTNAVIKE

LLRYRPPVIFVPYEATKKFPVTPNYTISKGSMIVPSCYPALHDPQVYPDP

ETFDPERWITGDAESKTKNWLVFGAGAHDCLARKYVPLTMAAMIGKASLE

LDWVHHATSQSEEIRVFATLFPEDECQLVFTRQG*

 

>CYP61A6 Nectria haematococca fgenesh1_pg.scaffold_25000074

CYP61A6

Necha1/scaffold_25:197180-198781

79% to CYP61A6 Fusarium graminearum , only 56% to 61A1 F. graminearum

intron at PKG motif in fgenesh1_pg.scaffold_12000391 not seen here

MIMDMQGSDVLHNGTLPAAPVPSNGVLTGYLANCSNWQVFFTILLLLMTYDQ (1)

VAYLIRKGSIAGPRFKIPFMGPFVQALYPKFDAYVAQWASGPLSCVSVFHK (2)

FVVLASDRDIAHKVFKSPTYAKPCLVPMATEIMGSNAWVFLMGKAHTEYRRGLNGLFSNKALGSYLPVQE

KVYEDYFGKFVEESEAHRGKPTAFMRLFREINCALSCRTFFGDYISQDAVQRIADDFYLVTAALELVNVP

LSIYVPFTKCWKGKRTAQAVLAEFAKCAAACKANMAAGGKPTCIVDQWVLHMMESKRYNERIAAGETGIE

KPTNLSREFTNEEIGSTMFTFLFASQDASSSATTWLFQVLAQRPDVLERLREENLAIRGGDRTRPFELSM

FESMTYTNAVVKELLRWRPPVIFIPYLATRSFPVTPNYTVPKGSMIVPSCYPALHDAKAYPDPETFNPDR

WITGDAEQKTKNWLVFGAGPHDCLARKYVPLTMAAMIGKAALELEWEHHATDRSEEIRVFATLFPM (0)

DECQLVFKRRE*

 

>CYP61A6 Aspergillus oryzae

AP007155.1

73% to CYP61A6 Nectria haematococca

        MDAKMATEIFNTTSAQPESPQSWAYKTSQLQGALPDFLAEWSAWQYVVTFLVGLVLYDQ (1?)

        VLYLKRKGALPGPTFKIPLMGPFIQAIHPTFDGYLRQWASGPLSCVSIFHK (2?)

672882  FVVLASDRDLAHKVFKSPAYAEPCLVPVARDIIGHKAWVFLQGRDHAEYRRGLV  673043

673044  PLFTSRAIATYLPVQERVLDDYYNQFVAATKANQGKPMAFMTLFREINCALSCRTFFGDY  673223

673224  ISDDQVKKIADDFYLATDALDLVNIPLAMYVPFTKTWLGKRTADAVHRVFAECAARCKAN  673403

673404  MAKGATPTCIVDHWVLHMMESNRYRERVAAGETNLEKPKNMIREFTNEEISDTLFTFLFA  673583

673584  SQDASSSATTWLFQILAQRPDVLDKLREENLAARGGDRNKPFDLPMLESLTYTNAVIKEL  673763

673764  LRYKPPVILVPYLATKDFPVTPDYTVPKGSMIIPSCYPALHDPEAYPNPDVFDPERWISG  673943

673944  DAESKTKNWLVFGAGPHDCLARKYVPLSLAGMIGKASLELDWEHHPTPRSEEIRVFATLFPM (0) 674129

        DGCNLVFKRRP*

 

>CYP61A6 Aspergillus flavus NRRL3357

AAIH01000781.1

Only 1 aa diff to 61A6 of Asp. Oryzae, 73% to CYP61A6 Nectria haematococca

AFL2G_02724 100% to CYP61A6 Aspergillus oryzae

      MDAKMATEIFNTTSAQPESPQSWAYKTSQLQGALPDFLAEWSAWQYVVTFLVGLVLYDQ

      VLYLKRKGALPGPTFKIPLMGPFIQAIHPTFDGYLRQWASGPLSCVSIFHR (2)

6085  FVVLASDRDLAHKVFKSPAYAEPCLVPVARDIIGHKAWVFLQGRDHAEYRRGLV  5924

5923  PLFTSRAIATYLPVQERVLDDYYNQFVAATKANQGKPMAFMTLFREINCALSCRTFFGDY  5744

5743  ISDDQVKKIADDFYLATDALDLVNIPLAMYVPFTKTWLGKRTADAVHRVFAECAARCKAN  5564

5563  MAKGATPTCIVDHWVLHMMESNRYRERVAAGETNLEKPKNMIREFTNEEISDTLFTFLFA  5384

5383  SQDASSSATTWLFQILAQRPDVLDKLREENLAARGGDRNKPFDLPMLESLTYTNAVIKEL  5204

5203  LRYKPPVILVPYLATKDFPVTPDYTVPKGSMIIPSCYPALHDPEAYPNPDVFDPERWISG  5024

5023  DAESKTKNWLVFGAGPHDCLARKYVPLSLAGMIGKASLELDWEHHPTPRSEEIRVFATLF  4844

4843  PM (0) 4838

      DGCNLVFKRRP*

 

>CYP61A6 Gibberella moniliformis

AAIM01002518.1

81% to CYP61A6 Nectria haematococca

also Fusarium verticillioides FVEG_08786

29769  MTSIVSPRHEAAISNASSTLKEDISNG

29688  NGLLSSYLNGYSNAQILLAILVVLIAYDQCMYLWRKGPIAGPAFKIPFMGPFIRALYPKF  29509

29508  DHYLAQWASGPLSCVSVFHK  (2) 29456

29396  FVVLASDRDIAHKVFKSPTYAKPCIVPMAETLLRPNAWVFLQGKAHTEYRKGLNGLFVN  29220

29219  KALSTYLPVQEKVYDDYFGRFVAASEANKGKPMAFMRLFREINCALSCRTFFGDYISQDA  29040

29039  VEKIADDFYEVTAALELVNVPLSVYVPFTKCWKGKRTADAVLAEFAKCAAACKANMTSGA  28860

28859  EPRCIVDQWVLHMMESKKYNDRIAAGEEGVEKPRNLIREFTDDEIGQTMFTFLFASQDAS  28680

28679  SSATTWLFQVLAQRPDVLDRLREENLAVRNGNRHQPFELSMLESLPYTNAVIKELLRYRP  28500

28499  PVIFVPYEATKSFPVTPKYTVSKGTLIVPTCYPALHDPQAYPNPETFDPDRWITGDAESK  28320

28319  TKNWLVFGAGPHDCLARKYVPLTMAAMIGKASLELDWVHHATSRSEEIRVFATLFPE  (0) 28149

28084  DECQLVFTKRE*  28049

 

>CYP61A6 Trichoderma reesei

AAIL01000244.1

75% to CYP61A6 Nectria haematococca

      MATIIEGSMPISFESTINATQHGTSGLVESLWSSCSAASNWQIAITALLVLVAYDQ (1)

10081 YLYISRKGSIAGPTFKIPLIGPFLQAIYPKFDAYLAQWASGPLSCVSVFHK (2) 9929

9869  FVVLASDRDLAHKVFKSPTFVRPCLVPMAINIMRPTAWVFLNGKAHAEFRRGLTGLFTN  9693

9692  KALSTYLPVQEKVYADYFNRFVEHSKANGGKPTKFMGWFREINCALSCRTFFGDYISQEA  9513

9512  VKRIADDFYLITDALELVNIPLSIHVPFTKCWRGKRTADAVLEEFTKCAAACKVNMASGA  9333

9332  QPTCIVDQWVQHMFESKRYNEAVAAGVEGLEKPTNLIREFSDEEIAQTLFTFLFASQDAS  9153

9152  SSATTWLFQILAQRPDVLDRLRAENLAARGGDKNRPFELEMLESLTYTNAVVKELLRYRP  8973

8972  PVIFVPYLALKPFPVTPDYTVPKNAMIIPSCYPALHDPAAYPNPDVFDPERWITGDAETK  8793

8792  TKNWLVFGAGAHDCLARRYVPLTMAAMIGKASLELDWVHHATERSEEIRVFATLFPM  (0)8625

      DECPLVFTRRE*  8517

 

>CYP61A6 Aspergillus terreus NIH2624

AAJN01000026.1

76% to CYP61A6 Nectria haematococca

ATEG_01083.1

       MNVKMQADMANSTQPDRQIWHVTTHWQGKVSDSFSGWSACQYIVTILLGVIFYDQ  (1)

44968  VMYIKRKGSIAGPTFKMPLMGPFLQAINPKFEEYLAQWASGPLSCVSVFHK  (2)45120

45171  FVVLASDRDLAHKVFKSPSYAEPCLVPIAKDILGPKAWVFLQGKAHAEYRRGLTPLFT  45344

45345  NNALATYLPVQDKVFATYFDKFVAASAANNGRPMEFMTMFREINCALSCRTFFGDYISQD  45524

45525  AVKKIADDFYLVTAALELVNIPLSMYVPYTKPWLGKRTADAVHAEFARCAAACKANMASG  45704

45705  AAPTCIVDHWVLHMMESNRYRERVAAGEANVEKPTNLIREFTNEEIGETLFTFLFASQDA  45884

45885  SSSATTWLFQVLAQRPDVLDRVRDENLAARGGDKSKPFDLPMLEGLTYTNAVVKELLRHR  46064

46065  PPVIFVPYLATKNFPVTPSYTVPKGSMIIPSCYPALHDPAVYPDPDVFEPERWISGDAES  46244

46245  KGKNWLVFGAGAHECLAKRYVPLSMAAMIGKAALELDWTHHATERSEEIRVFATLFPM  (0) 46418

       DGCQLVFRKRS*  46499

 

>CYP61A6 Chaetomium globosum CBS

AAFU01000675.1 148.51

74% to CYP61A6 Nectria haematococca

 796  MAADHVNASSSTPELLMSSSGLASGSSSWQFRDGVVGITAEWSAWQYVITLLLGLVVYDQ (1) 975

1031  VMYLYRKGSITGPRFKVPLTGPFIQALHPKFESYLAQWASGPLSCVSVFHK  (2) 1183

1242  FVVLASDRELAHKVFKSPTYTEPCIVPLAKDILGHKAWVFLQGKAHAEYRRGLTPLFT  1415

1416  NKAMATYLPAQEKVFADYFDKFVAASQANVNQPLPFMTLFREINCALSCRTFFGDYISPA  1595

1596  AVKKIADDYYQATAALELVNVPFSIYIPFTKPWRGKRTADAVHAEFAKCAAACKRNMATG  1775

1776  AAPTCIVDHWVRHMFESNRYRERVAAGETDVEKPSNLIREFTDVEISETLFTFLFASQDA  1955

1956  SSSATTWLFQILAQRPDVLDRLREENLAARGGDRNKPFDLAMLESLTYTAAVVKELLRYR  2135

2136  PPVIFVPYLATKAFPITPDYTVPKGAMVIPSCYPALHDPKVYPNPDTFDPDRWITGDAES  2315

2316  KTKNWLVFGAGAHDCLARRYVPLTMAAMIGKAALELNWKHVATEKSEEIRVFATLFPM (0)  2489

      DGAQLVFEKRP*  2579

 

>CYP61A6 Aspergillus clavatus NRRL

AAKD02000011.1

77% to CYP61A6 Nectria haematococca

ACLA_008020

        MDTEIANVSRAAAQSGSPQAWT

669433  PQPSSGQNTLLDCLAGWSTWQYLVTFLLGIVVYDQ  (1)

669274  VMYLKRKGSIAGPAFKIPLMGPFLQAIHPKFDAYLAQWASGPLSCVSVFHK  (2) 669122

669053  FVVLASDRDLAHKVFKSPSYAEPCIVPIAKDIIGHKSWVFLQGKAHAEYR  668904

668903  RGLTPLFTNKAMATYLPVQEKVFTDYFDRFVTASQANRGRPMAFMSMFREINCALSCRTF  668724

668723  FGDYISQKAVKRIADDFYLATAALELVNIPLSMYIPFTKTWLGKRTADAVHVEFAKCAAA  668544

668543  CKANMATGAKPTCIVDHWVLHMMESKRYHERVAAGETDVEKPTNLIREFTNEEIGETLFT  668364

668363  FLFASQDASSSATTWLFQILAQRPDVLDRLREENLAARGGDRNRPFDLPMLESLSYTNAV  668184

668183  IKELLRHRPPVIFVPYLATKNFPVTPDYTVPKGSMVIPSCWPALHDPDVYPNPEVFEPER  668004

668003  WISGDAESKTKNWLVFGAGPHECLAKRYVPLSMAAMIGKAALELEWTHHATEKSEEIRVF  667824

667823  ATLFPM (0)

        DGCQLVFSKRV*  667698

 

>CYP61A6 Fusarium oxysporum

96% to CYP61A6 Gibberella moniliformis

FOXG_09685 revised

MASIVSPGDEAVVSNASLTFKDDISNSNGLLSSYLNGYSNTQILLTILVVLIAYDQCIYLWRKGPIAGPAFKIPFMGPFI

RALYPKFDHYLAQWASGPLSCVSVFHKFVVLASDRDIAHKVFKSPTYAKPCIVPMAETLLRPSAWVFLQGKAHTEYRKGL

NGLFVNKALSTYLPVQEKVYDDYFGRFVAASEANKGKPMAFMRLFREINCALSCRTFFGDYISQDAVEKIADDFYEVTAA

LELVNVPLSVYIPFTKCWKGKRTADAVLAEFAKCAAACKANMASGAEPRCIVDQWVLHMMESKKYYDRIAAGEEGVEKPR

NLIREFTDDEIGQTMFTFLFASQDASSSATTWLFQVLAQRPDVLDRLREENLAVRNGNRHQPFELPMLESLPYTNAVIKE

LLRYRPPVIFVPYEATKSFPVTPKYTVSKGTLIVPTCYPALHDPQAYPNPETFDPDRWITGDAESKTKNWLVFGAGPHDC

LARKYVPLTMAAMIGKASLELDWVHHATSRSEEIRVFATLFPE (0)

DECQLVFTKRE*

 

>CYP61A7 Aspergillus niger

estExt_fgenesh1_pm.C_40133|Aspni1

74% to CYP61A5

MEANSHSFASPLASVRASNLPIPSQFETVVDAVSNASPWTIAFTILAVLVAYDQIIYLINKGNIVGPAFKIPFIGPFLES

VNPKFEEYYAKWTSGPLSCVSVFHKFVVIASTRDLARKVFNSPTYVKPCVVDVAHKLLGKENFVFMDGKPHVDFRKGLNG

LFTKKALQSYLPGQEDVYNQYFERFLGITQKAGGKPVPFMPEFRELMCAVSLRTFCGYYISDEAVKKISDDYYLITEAME

LVNFPIILPFTKAWYGKKASDMVMAEFCQASAKSKVCMAAGKEPTCIMDAWVKNILASKQWVEAEAKGQLAENMEKPSPL

LRDFADWEIAQTVLAFLFASQDATSSAATWLFQTMAQRPDVLDRVREENYKVRNGNVHAEVDMDQLESMTYTRAVVRELL

RYRPPVIMVPYVAKKPFPITDSYTVPKGAMVIPTTYLALRDEEVYEKPDEFDPERYYTGDAEEKGKNNYLVFGTGPHVCI

GQHYAQLNLVLLIGKASLLLDWKHHATPLSEEIKVFATIFPKDDCPLTFNKRKW*

 

>CYP61A8 P450-49 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

93% to P450-10_var1

53% to CYP61A3 S. pombe in CYP61 family

RO3G_16941.1

MSASSTSLFNPRSIESAKNAINSFVRSALNTQPSTTVLVCSALVGLFAYEQYVYLKKKRSLPGPLLKIPIIGAFMDSLYPTFEGYMSKWRSGELSCVSVFDRFVVIASTCELSRKILNAPAYAEPAVVNSMRHILCADNWVFLSGKAHVDYRKGLNVLFTRKALGIYIPIQEKVYQKHFDEWLSLDGRSEQYQLRFREINMEASLRVFLGEYMSDAVATQISQEYFNITAALELVNFPIPLPGTKVYKAIQSRKFIVKHFIECIKDSRIRMAQGGEVKSMMDAWISSMNEYEVECEKAGKPAPRKFDDREVALTILTFLFASQDATSSALTWAFQLLADHPDVLEKVYQEQLRLRKDNLQQELSLELMDASTYLRQVIKEILRLRPPVLMVPYQTVREWPITPNYTIPKKSMVIPTTYPALHDPVAYPNPDAFDPERWGPEGIAEKYPKNFMVFGNGPHHCLGKEYAMMHLMAVISQATLQMKWTHTRTEKSDDISIFATIYPQDGCIMSFKPRN

 

>CYP61A9 P450-8 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

50% to CYP61A3 S. pombe = CYP61 C-22 sterol desaturase

RO3G_04483.1

MDSVFIEKARKLFEKTVEQIKEVIAEGLAADKAYFTPIGLASAAVAGFLVWEQVDYHRKKRHLPGPSFKMPLIGSLMDSMEPTFDKYNSKWKSGNLSCVSVFNRFIVIASNCELSRKILNSPGFVQPAVVDSMRYILTDDNWVFMDGKEHLNYRKGLHSLFQRSSLGGYLTIMDKCYQKFFDEWLSHEGKTRRYQWDFRELNMQSSLRVFLGDFMPESVAKKCSEEYFNITAALELVNFPYPLPGTKIYKAMKSRQYIYDAFVESIKDARIRMSQGSQTTCLLDNWLVTMKENERECLEAGKPAPHVFTNHEIALTILTFLFASQDATSSALTWTFQLLADHPETLKKVYDEQDKLRADDPNQPLTLELMEKSVYLRQVVKESLRLRPPVLMVPYQTHRDFPISDGYTVPANSMLIPTTYPALHDPVAYPNPDAFDPDRWGPEGVAERHPKNFMVFGNGPHHCIGKEYAILHVMSTVAQATRRLEFKHYPTSESDTIWLFATTYPKDECSMSFHPRTKFPYH*

 

>CYP61A10 Aspergillus terreus NIH2624

NW_001471411.1 AAJN01000002.1

72% TO 61A1 A. ORYZAE, 55% TO A. TERREUS 61A6

70% to CYP61A1 A. niger

formerly CYP61A1, but a better match was found in A. terreus to CYP61A1

ATEG_00175.1

247642  MDFISKNTSPIIQKFQSPGIVAIFGSDISIWMVLLTVLITAVVYDQ (1) 247780

247843  FKYIRLKGTIAGPSWKIPFMGPFLESMDPKLEGYMAKWRSGELSCVSIFHK (2) 247997

248055  FVVIASTREMSCKIFNSPMFVKPCVVDSAHKLLGKDNWVFLDGKEHVDFRKGLNLLFTR  248228

248229  SALACYLPRQEEVFDMYFERFVARSAANNYKPLPWMPEFRKLMCTISCRTFVGHYISESA  248408

248409  LDKIAHDYYLITAALELVNFPIVLPFTKSWYGKRAADMVLREFSQCAAKSKLHIASGGEI  248588

248589  TCIMDAWVKAQLDSAAYRKNVAAGIPCEKPSHLLRDFTDYEIAQTVFTLLFASQDAN  248759

248760  SSAITWLFQIMADRPDVLDKLREENLRARNGDCNARLSMELFDKLPYTRAVVRETLRYRP  248939

248940  PVILVPYIAKKDFPITEKYTIAKGSMVLPSIWPATHDAEAYPNPDSFDPDRWITGTAEQH  249119

249120  PKNFLVFGTGPHYCLGQTYVQMNLMAIIGKASLALDWKHHITPDSEQIKVFATIFPQ (0)  249290

        DDCLLTFRRQPGS*

 

>CYP62A1 Aspergillus nidulans AN7824.1 stcB Emericella nidulans U34740

not a complete match 53 clan

MISQICNEVIGLVPKKEEPGWLSVTGHPGAVYCEN

SISSGPEATGAD

VSQAIRIAYFTPLKHIPGPWYASLTGLRLSWSVFANNRIHYVHSLHQKYGPIV

RIGPQEIDVADPVAGREIHRMGSGFMKAPFYELLSPGPVDNIFNFRDPKL

HAARRKLYARGFTLQSLRNEWEPKVRDIIKLTVEKIKCDAVKGEAEIMGW

WTLMANEIVCQLTFGGGAGIVAKGVKEPFVLMLERRMGDLAHLLKHFAPP

GYYLGRALAWFIPPLQDIFYSQERMFAAGGDVVSRAREAKKAQAEPRNLF

NKALEAGNLTDTDIITDAGALLLAGSDPTAISLTFLLWCVLSRPEVQKQV

EAEVATLEGELTDEACERLPILNAVIDESLRLYGAAPGCMPRSPPSGGVT

IGGYFIPDDTIVATQNWSLQRNPSIWDDADTFDHTRWLSNSRITDQAKLA

FNPFGYGARQCLGIHLGRMEMRLAAAMFFRECVGARLGRSVTDESMHVVD

SFIAGVPRDRRCAITLT*

 

>CYP62A1     Aspergillus parasiticus aflatoxin pathway gene cluster

            GenEMBL AY371490

            complement(join(62552..62810,62866..64025,64093..64200))

            gene="aflV

MTNTAPRELIRAIEHVPLTWWFLAVGGAWIVSKIIKILQTAYFS

PLRKIPGPWYARLTSARLAWASFANNRIYYVQSLHDKYGSIVLIGPEEVDIADPVAAK

QIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTRRKLYAKGFTLNSLRQQWEPT

IRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNGTKDPFVLM

LERRMGDLAHLLQHFAPPLYYLGRLLGRAVPRLHDVFFSQETMFEAGKHVVAIARSAR

DAEGDRNLFVKALAAGDLESKIGGLNDTEIITDAGALLLAGSDPTALSLTYLIWCVLN

RPKLQAELESEVAGLQGDITDAACADLPILNAVIYESLRLYGPAPGAMPRSPPPDGAT

LCGYYIPPSAVVVTQNWSLHGSPKVWKDPHTFDHTRWLPGSSLSEEAKISFNPFGQGA

RQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPESMVMIDSFIAGMPKARRCAIQL

 

>CYP62A1  Aspergillus oryzae

         GenEMBL BAE71332.1, BAE59529.1, BAC20333.1

         66% to CYP62A1 Aspergillus nidulans, ortholog

MTNTASRELIRAIEHVPLTWWFLAVGGAWIVSKIVKILQTAYFSPLRKVPGPWYARLTSARLAWASFANN

RIYYVQSLHEKYGPIVLIGPEEVDIADPVAAKQIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTR

RKLYAKGFTLNSLRQQWEPTIRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNG

TKDPFVLMLERRMGDLAHLLQHFAPPLYYLGRLLGRAEPRLHDVFFSQETMFEAGKHVVAIARSARDAEG

DRNLFVKALAAGDLESKIGCLNDTEIITDAGALLLAGSDPTALSLTYLIWCVLNRPKLQADLESEVAGLQ

GDITDAACADLPTLNAVICESLRLYGPAPGSMPRSPPPDGATLCGYYIPPSAVVVTQNWSLHGNPKVWKD

PHTFDHTRWLPGSSLSEEAKMSFNPFGQGARQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPESMVM

IDSFIAGMPKARRCAIQL

 

>CYP62A1  Aspergillus flavus

99% to CYP62A1  Aspergillus oryzae

AFL2G_07212

MTNTASRELIRAIEHVPLTWWFLAVGGAWIVSKIVKILQTAYFSPLRKVPGPWYARLTSARLAWASFANNRIYYVQSLHE

KYGPIVLIGPEEVDIADPVAAKQIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSTRRKLYAKGFTLNSLRQQWEPT

IRNIVALTVERIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNGTKDPFVLMLERRMGDLAHLLQHFAPPLYYL

GRLLGRAEPRLHDVFFSQETMFEAGKHVVAIARSARDAEGDRNLFVKALAAGDLESKIGCLNDTEIITDAGALLLAGSDP

TALSLTYLIWCVLNRPKLQAELESEVAGLQGDITDAACADLPILNAVICESLRLYGPAPGSMPRSPPPDGATLCGYYIPP

SAVVVTQNWSLHGNPKVWKDPHTFDHTRWLPGSSLSEEAKMSFNPFGQGARQCLGIHLGWMQLRLATALFFRRCPGAKLA

PSTTPESMVMIDSFIAGMPKARRCAIQL*

 

>CYP62A1 Aspergillus nomius

AY510454

complement(join(68188..68446,68501..69660,69727..69834))

gene CypX of aflatoxin biosynthesis

92% to CYP62A1 Aspergillus flavus
MINTPPRDLIRAIEQVPLTWWFLTVGGAWILSTIVK ILQTAYFS

PLRKVPGPWYARLTSARLAWASFANNRIYYVQSLHDKYGPIVLIGPEEVDIADPGAAK

QIHRMGSGFVKAPFYKLLSPGPVDNIFNFRDAKLHSNRRKLYAKGFTLNSLRQEWEPT

IRNIVALTVEKIRHDAQQGEAEILGWWTLMANETVCKLTFNGGHDTVRNGTKDPFVLM

LERRMGDLAHLLQHFTPPLYYLGRLLARGVPRLHDVFFSQEMMFEAGKSVVAIARGAR

GGEGDRNLFVKALAAGDFESKIGGLNDTEIITDAGALLLAGSDPTALSLTFLIWCVLN

RPKLQAELESEVAGLPEDITDAACAELPIMNAVIHESLRLYGPAPGSMPRSPPPDGAT

LCGYYIPPSAVVVTQNWSLHGNPKVWKDPHSFDHTRWLPGTSLSEEAKMSFNPFGQGA

RQCLGIHLGWMQLRLATALFFRRCPGAKLAPSTTPETMVMIDSFIAGMPKARRCAIQL*

 

>CYP62A1 Aspergillus ochraceoroseus strain SRRC 1432

AY092402.3

join(18386..18418,18503..19650,19718..19979)

gene StcB/AflV in aflatoxin/sterigmatocystin biosynthesis

80% to CYP62A1 Aspergillus nidulans

N-terminal exon modified to be more like Podospora anserina EST CU876063

MDFFTSLGSTQWLSILVYVGGLWLLVKVFQVSL (0)

TLRIAYFSPLSRIPGPWYARLTGLRLSWSVFAN

NRIHYVHKLHQDYGPIVRIGPQEVDVADPALGREMHRMGSGFTKAPFYALLSPAPVDN

IFNFRDAKLHAARRKLYAREFTLQSLRKEWEPTIREITKMTVQRIKHDALNGEAEIMG

WWTLMANEVVCRLTFGGGAGTVAKGVKEPFVLMLERRMGDLAHLLKHFAPPGYYLGRM

LAHVIPQLHDVFYSQEKMFAAGGGVVSRARAVKRDGDDSGPSNLFNKALEAGSLTDTD

IITDAGALLLAGSDPTAISLTFLLWCVLSRPQIQHEVEAEVAQLEGEITDAACEGLPI

LNAVINESLRLYGAAPGCMPRSPPPGGATLGGYFLPADTVVVTQNWSLQRDPNIWENA

HSFDHTRLPREIRMTEQAKIAFNPFGHGARQCLGIHLGRVEMRLATAIFFRECAGARL

SESVTEESMQVVDSFIAGVPRDRRCTIRLG*

 

>CYP62B1 Fusarium graminearum FG05806.1 AACM01000233 FGcontig1.233_scaffold3

MTLITTPGAPLLAGVFLFVIISYTLVTSLSSPLRRVPGPWYTHFTHFVLK

FHIVTGNRIHYVHSLHQRYGPIVRLTPWEVSVSDAEAVSAVHKIGAGFLK

SAWYDGITPGREPGIFAMRDPHQHSARRRLFARAFSNGSLLTNWEPEIRQ

KTSLAVQKIKQDALGAGADVFKWWTLMATDVIAHLSFGESFHMLEQGKQT

RYIDSIQLAMINSGIRTELSWIYPFLKFIPSQGLKTLLNADNVVYDHGTV

AVRNMQSAGDGLSRANLFSQMVAEADGQEKTTLTASSVQAEAGNLIIAGS

DTTAVTLTYLVWAVLKHPQLQARLEEEVGSLSDELTFDELKNAPLLNSII

EETLRLYGAAPGALPRVVPGKGATLVGQYLPGGTVVSTQAYTFHRDENIW

PDALRFDGYRFMDKSKMTASQKAALSPFGAGSRICIGMHLAYMELRLGAA

LFFRECRGVKIGVEMTDEMMEMENHFLIAPKTHKCIVKL*

 

>CYP62B2 Nectria haematococca

fgenesh1_pg.scaffold_23000205

Necha1/scaffold_23:519115-520695

66% to CYP62B1 Fusarium graminearum

This gene model seems correct DRN 2/6/06

MGLVNIALVPFLAIGVIILTAAYRLFSAATSPLRNVPGPWYSHVTSIVLKWHILAGRRVHFVHSLHLRYG

PVVLVSPDEVAISDLDAFSQIHKIGSGFTKSDWYDKLRSGREPGIFFMRDPHQHAARRRLFARAFSNSSL

LTHWEPEIRQKTQLAVAKIKNDAQIGSADVLKWWTLMATDVIAHLSFGESFRMLELGK (0)

QTPYIDAIQAALLCGVVRNELSWIFPIIRRLPFKGIRDIMSADDVVFEHGAKAVKNMSSASDGLSTANLF

SQMLAQAQGQEKTEIKDSTVRTEAGNLIVAGSDTTAVTLTYLVWAVLSQPQLQAALEAEVCGLSPDLTFD

ELTSAPLLNSVVEETLRLYGAAPGALPRVVPGKGLSVRGYFIPPGTVVSTQAYTLHRDSTVFPDAHR (2)

FDGERFMDKSTLTVAQKTAMSPFGAGSRICIGLHLAWMELRLATALFFRECRGAK

LDPGMTDDMMEMDNRFLIAPKGHCCKIQL*

 

>CYP62B3 Fusarium oxysporum

76% to CYP62B1   Fusarium graminearum

FOXG_07404

MYIANYLSMPLLAGVFLIGIVLYALVNAFKSPLRGLPGPWYTHFTHLVLKWQILAGNRVHYIHSLHQHYGPVVRVSPGEVAVSDLEAF

SKIHKIGSGFLKSAWYDGITPNREPGIFVMRDPHQHAARRRLFARAFSVSSLLTNWESEIRQKTELAVNNIKRDAQGAGA

DVFKWWTLMATDVIAHLSFGESFRMLELGKQTPYIDAIQSALLMSGIRAELSWIYPLLQRLPFQGLKKLLNADNVVFDHG

AIAVQNMQSAGDGFSKANLFSQMLAESDSQDKTNLSLLSVQQEAGNLIVAGSDTTAVTLTYLIWAVLKQPQLQAELEREI

SELSDELSFDELKSAPLLNSVIEETLRLYGAAPGALPRVVPGKGLDVCGHYIPPGTVVSTQAFTLHRNENIFDDAQR (2)

FNGYRFMDKSTLTGAQKTALSPFGAGSRICIGLHLAWMELRLGAALFFRECRSATLSPEMADEMME

MENRFLIAPKGHRCIVKL*

 

>CYP62B3 Fusarium verticillioides

90% to CYP62B3 Fusarium oxysporum = ortholog

FVEG_04329 revised

MYIANYLNIPLLAGAFLAGIVLYTLVNAFKSPLGGLPGPWYTHFTHLILKYHILS

GNRVHYIHSLHQRYGPVVRVSPGEVAVSDLEAFSKVHKIGSGFLK

SGWYDGITPNREPGIFVMRDPHQHAARRRLFARAFSVSSLLSNWESEIRQKT

ELAVNNIKRDAQSTGADVFKWWTLMATDVIAHLSFGESFRMLELGKQT

PYIDAIQSSLLMSALRAELSWIYPLLKRLPFQGLKKLLNADSIVFEHGSI

AVRNMQSAGDGLSKANLFSQMLAESDSQEKTSLSTSSVQAEASNLIVAGS

DTTAVTLTYLIWAVLKQPQLQAELEREISELSDELTFDELKSAPLLNSVIE

ETLRLYGAAPGALPRVVPGKGLDVCGHYIPPGTIVSTQAFTLHRNENIFEDAQR (2)

FNGHRFMDKSTLTAAQKAAFSPFGAGSRICIGLHLAWMELRLGAALFFRECCGASLSPE

MTEAMMDMENRFLIAPKGHKCVVKL*

 

>CYP62B fragment BI188839 Fusarium sporotrichioides EST 88% to 62B1

PRVVPGKGATLAGHYLPAGTVVSTQAYTVHRDESIWPNALRFDGYRFMDKSRVTATQKSA

LSPFGAGSRV

CIGMHLAYMELRLGAALFFRECRGVKLGVEMTDEMMAMENHFLIAPKAHKCMVKL*

 

>CYP62C1 Aspergillus nidulans AN6414.1 49% to 62B1 53 clan

MMERSWKYIAIACALWVVQ (0)

IYSAITSPLRAIPG

PFYTSLTRLPLKLSIIAGQRIYFIHSLHQRYGPIVRVSPTEVSIASLPEF

REIHRVGSPFLKSNWYEKFVMGQHSPGVFAISDPKQHGARRRLFARAMSN

TELRRVWEDVVRSKVRLAVDRIKGELEADGARCDVLKWWTFLATDVVGHL

MFGEDFDMLNIGVKNEYIHVLESTMKGSGINAELPLVGCIGRHLPFSVVR

SMFRANDYLTNYGKRAVTNARAKSDSSRNIFSGMLYEAEKCTDSDSGLSE

LDIITEAGNMIVAGSDTTAVTLTYLVWVVLSRPQLQREIESEVLALEEGY

DDAKLETLPVLNAVIKETLRLYGAAPGSLPRTVPRGGATLGGYFIPEGMT

VSTQSWTVHRDENLFPDPDSFNISRWLEENDAGFPAAQLAFSPFGAGGRI

CLGIHLAYMELRLAAAEFFRRCRGVRLSPDTTWKIMK

PMNYFLIAPTGNRCDIMRG*

 

>CYP62C2  Aspergillus flavus NRRL3357

          GenEMBL AAIH01000225.1

          63% TO 62C1

          1 AA diff to Aspergillus oryzae CYP62C2

          AFL2G_00193

     MEWQIYLGIAFALWIVQ (0)

955  VLYTAFTSPLRRVPGPLYTVLTRLPLKLASLTGNRIYFVHELHRKYGPIVRIAPDDVDISS 1137

1138 LAEFREIHRAGSPFLKSKWYEKFVPSKRSGVFTMRDPKEHAARRKLFARPFSKSELRRT 1314

1315 WEPAVREKVQLAVSQIQRELKAVGKSDLLKWWTFLATDVSGQLMFGESFNMLQLGK (0)1482

1535 KNQYINVLESTMMGSGIGAELPLVAWLGRHIPLSSFQNMFRATDYLFQYGQRAVTNSRTT 1714

1715 SNASRNIFAGMVYESEKGDGIITDEEVVLEAGNLIVAGSDTTAITLTYLIWAVLSQP 1885

1886 KLQRELEEEVNSLSADFDDSALEELPLLNAVIMEALRLYGAAPGALPRETPEGGAKFCGY 2065

2066 FIPQGMTVTTQAYSIHRDGDIYPDPERFDVSRWLKTETSASELAKKAFSPFGAGGRICLG 2245

2246 IHLAWMELRLATAQFFRECAGVRLAPSATWENMRPVNYFLISPRGHQCEVECNY* 2410

 

>CYP62C2  Aspergillus oryzae RIB40

          GENEMBL AP007151.1

        MEWQIYLGIAFALWIVQ (0)

476019  VLYTAFTSPLRRVPGPLYTVLTRLPLKLASLTGNRIYFVHELHRKYGPIVRIAPDEVDISS  476201

476202  LAEFREIHRAGSPFLKSKWYEKFVPSKRSGVFTMRDPKEHAARRKLFARPFSKSELRRTW  476381

476382  EPAVREKVQLAVSQIQRELKAVGKSDLLKWWTFLATDVSGQLMFGESFNMLQLGK  (0) 476546

476599  KNQYINVLESTMMGSGIGAELPLVAWLGRHIPLSSFQNMFRATDYLFQYGQRAVTNSRTTS  476781

476782  NASRNIFAGMVYESEKGDGIITDEEVVLEAGNLIVAGSDTTAITLTYLIWAVLSQPKLQ  476958

476959  RELEEEVNSLSADFDDSALEELPLLNAVIMEALRLYGAAPGALPRETPEGGAKFCGYFIP  477138

477139  QGMTVTTQAYSIHRDGDIYPDPERFDVSRWLKTETSASELAKKAFSPFGAGGRIC  477303

477304  LGIHLAWMELRLATAQFFRECAGVRLAPSATWENMRPVNYFLISPRGHQCEVECNY*  477474

 

>CYP62C3 Aspergillus niger

estExt_fgenesh1_pg.C_150110|Aspni1

67% to CYP62C2

MERQVALCAATAVAIWVLQIAYKLLRSPLRHIPGPLYTRVTRLPLKFSIVTGRRIYFIHNLHQKYGPVVRIAPDEVSVSS

LPEFKEIHRVGSAFLKTPWYQKFAWDRNPGLFTMRDPKEHAARRKLFARVFSKSDLRRTWEPEVKDKVQLAVSQIRGELL

RDGISDILKWWTFLATDVSGHLMFGESFDMLQYGK

VSYQSDYPCMRGSANCQ

KNEYINVLESTMMGSGINVELPIVGLLG

RYVPLASCQAMFRPSDYLTEYGRRAVSNSRTNSNSTRNIFTGMLNESEKASSMLSEEDVVIEAGNLIVAGSDTTAVTLTY

LVWAVLSQPTLQHDLEEEVSALDPNYNEAALEELPLLNAVITETLRLYGAAPGSLPRSVPNGGATFCGYHVPQGATVSTQ

SYTIHRDEELYPDPETFDATRWLGGHGNGSDVAKQALSPFGYGTRVCLGVHLAWMELRLAATEFFRECRGVRLASDTTRE

SMKPENYFLIAPSGHR

YSSSSGRKRMSGEEGGWFYGDSGDPVIRFGMMDRTGDQCQMDLQNDWVDAEPKQGCPCFRAVGP

TAASAEATWNHEKKTITRSSPPTTRGLAGLLNSPPPDLNKPEQQVTYRGSGRTGGCCQACCKGAALVRHEANPTSTLSPN

VAPLRGVSLPVGI*

 

>CYP62D1 Nectria haematococca

fgenesh1_pg.scaffold_70000012

Necha1/scaffold_70:35727-37407

MMGLPGYQAILSIIGAALLAGWL (0)

VIGRAYLTPLRAIPGPWHAPLTCLWLKKQTIAGRRAHYVHELHLKFG

SVVRIAPEEISFNDTGAFKEIHRISGNYVKSPWYQCFRKGTTTDLFSMTNPREHAQRRKLFAPLLSNSAL

MRNWFDTIVDKVDVTIEKMKRQQLQEGQVDIFKWWTFMTADVISHLAFGEPLGMLEQER (0)

HMQKIEDSTKLGLVGAELPFVRSLLEWTPIRSVRDIVNADVEVQSLATDVMTKTLRDGIGTTNIFSKMVA

EKEREAQALSDYQLAYEAGGLIVAGSGTTAVSLTYLVWAVLSQPAVQARLESELEKLPAGFSDSDLEALP

YLAAVIEETLRLYGAAPGALPRVVPEGGAKLAGYFIPQGIIVSTQAYTYHRDPEAYPEPEN (2)

FVPERFLNASGQFKAPKTGVYAPFGAGSRTCIGIHLARIELRHGACFFFRECKG

ARLAPQTTPLSMEMVNYFLISPRSEQCWVTLSN*

 

>CYP62E1 Phaeosphaeria nodorum SN15

AAGI01000353.1

46% TO 62C1, 46% TI 62B1, 42% TO 62D1, 39% TO 62A1

MAFVDVVTANKPLLVALI (0)

FVYVGLTCPTRHLPGPWYTRFTHYRLKRAVVTGQRIFYIDALHKQYGPIVRLSPTEV 956

GVADLDAFKEIHKIGTKYMKSEWYLRLANFPKAGVFTMLDPREHGARRKLLSRSFSRSY 1133

LVENWEPAVREKALLVVTKIKVDAMQSTADVYNWWMLLASDVSAHLAFGESFRMLETGH (0) 1307

ANQFIRVLKKLTMGAGIMVEMPFLRLLRFVPINAVQEMFNANEFILTGAGRAVEMARSRT 1542

GESNIFAKVIEDCEKEGEGHIDDMDVRIEAMNIIIAGTDTTGVTLTYLTWAVLQQPQ 1713

LQSALEVETAGLRENFTESDLINLPLLNATIEETLRLYGAAPSSLPRVVPQGGTRFSGHY 1893

IPQGVTVDTQAYTFHRDPRIWSDPLTFDPQRWISSQEGYTAEALSPDAKTAFHPFGVGAR 2073

SCIGIHLARMELRYAVAFFFRECRGIRLAPSTTLESMEFENFFLIAPKAHRCDITFQQ*

 

>CYP62F1 Mycosphaerella fijiensis

40% to CYP62B2   Nectria haematococca

41% to CYP62B1   Fusarium graminearum

e_gw1.7.207.1 revised at N-term

MELFLLGAIAAFLGLLYKV (2)

RKIVIHARNALPHIPGPWYTRYTSLVFYINGFIPRVPVWVDNLHRQYGPVVRITPNMVAIADPDGVADIFRSSRGYLK

GPIYDSFLRSFFAERNPALHGQRRRLFARPLANSSLQASWAPEIRQRVALAVKRIKDDALATGEGDAYKWWNIMTNDVIT

RISFGESLNLLESDEHLPYIEAVKKTIRCVSLHRNFPRIMPLAKYLPIRRLQEILNARPKAEKYTVKAVQNFRARIDKDQ

DKTAGTARNIFTQIVVQADDQDAEAVLAITDEDVHEEALTLMLGGTDSTSSTLTYCTWAVLRHPEIREELEKEVAALSDE

LSSKELEQARLLNAVIHETMRLYGAVSIGLQRVVPASGAQIAGFDLAPGTLVWPQMYTTRYDPSIWPDPLKFDPSRFFDV

KLPSMAEGNLTATQKKVYVPFGGGTRMCLGINLAYLELRLGLALFFRECKGARLSKSMTAEEMVHDNFFVSSPKGRRCMI

TLG*

 

>CYP63A1 Phanerochaete chrysosporium (white rot fungus) AF005475

Scaffold_293a 98% to CYP63A1 gene model complete all boundaries checked

MGLTQAQRLVLGQLARLVAPALAVCVLLAAARRTQLVRAPVWADALIALIAIPLFHVGRA

HWRYARLARKAARLGAALPPRWEGKLPGSVDVLQLVDEAYRHGFLS

DYFYEKFGELGHTYNFYVLWDMDYCTEDAAVIK 

AVLATDFNNWVK 

GERFDSYMHSVLGTGVFNAD

GELWK 

FHRSMTRPFFARERITDFETFNRHAEEAILKMKERLREGFAVDFA

DLISRFTLDAATEFLFGACVHSLAGALPYPHGAPAHLHTTRARIPADDFAAAFRAAQDAV

SHRARLVWLWPWFELARSRTDAPMRTVDRYLTPIIERALAMSRAAKQAPQGEKEEVADGE

TLLDHLARYTT

DPTILHDEILNIMIAGRDT 

TAGTLTFVVYFLTQHPNVLQRLRQ

EILDVVGPSNLPTYDDIKQMKYLRAVLN 

ETLRLYPPV

PWNMR 

YAVKDGILPNSEPDGKPWFIPAGAS

VSYSVHCMHRRKDYWGPD 

AEEFDPDRFLDERLHKYLTPNPFIFLPFNAGPRICLGQQFAYNEMSFFLVK

LLQTFEDISFERDAFEPNALPPAEWAKFPGRKGKEKFWPRAHLTLYSE 

GGMWVKMREAQAMGPQVA*

 

>CYP63A2 Phanerochaete chrysosporium

AY835607

cytochrome P450 monooxygenase pc-2

Scaffold_301b

MLVSVDALALRTLVYELTYLLYPAVPTAAALLLLQRLGSVWLPT

WTIVLLSLCSVPVAHRILLWLKDWRAARKAASMGAILPPRLKGRWPGSIDLLRQLTKT

FETGFLSEMLWGYMHVLGQTFEVYILWDSNYVTSDANVIKTILATDFDNFVKGEKLDV

CVRPVLGTGVFNSDGEMWKFHRSMTRPFFTRERISHFDLFDRHADATMAKMKARLAEG

FAVDFQDLISRFTLDSATEFLFGQCVHSLASVLPYPHDAPAHLQTTGASRTEDFARAF

AEAQDAVSFRLRMGWLWPWFELFGSRTKAPMAVVDAFLDPILRDAVARADKIKRENGG

RVPEVKGEIEEDETLLDHLVNVVQTKILHDEVLNIMIAGRDTTGGTLTSAVYFLSQYP

EVLRRLREEILEKVGPTRRPTYDDIREMKYLRAFINETLRLYPAVPWNVRYPVKDTTI

PGPHPDKPYFIPANTPVSYSVHCMHRRTDYWGPDAEAFDPDRFLDARVQRYLTPNPFI

FLPFNAGPRICLGQQFAYNEMSFFVIRLLQHFDEVQLCEDALAPDCRVPDAWRGAPGR

KGVERFWPKAHLTLYAKGGLWVKMREASTSEAVV

 

>CYP63A3 Phanerochaete chrysosporium cytochrome P450 monooxygenase pc-3

AY255625

69% to CYP63A1

MPSSIDFPDRLVLRVIAYELVFLFYPAVPAAAGLVLLRRLTDIW

LPTWAIVLLSVCSLPVVHGLSIWRNHWRAARKAARMGAVLPPRLKGRWPGSIDLLMRL

TDAFETGFMSDLLWEYMHTIGQTFEVYVLWDSNYVTSDANVVKAILATDFTSFVKGKK

FDVCMRSVLGTGVFNSDGDMWKFHRTMTRPFFTRERISHFDLFDRHADDAMAKMKARF

AEGYAVDFQDLISRFTLDSATEFLFGQCVHSLASVLPYPHNAPAHLQTTSASAAEDFA

RAFAEAQTVLNFRIRMGWLWPWFELFGSRTKAPMAVVDAFLDPILKAAVERADKIKHA

NGGKVPEAKEEIDEDETLLDHLVKYTNDPKILHDEVLNIMIAGRDTTAGTLTSAVYFL

SQYPEVLRRLREEILEKVGPTRRPTYDDIREMKYLRAFINETLRLYPAVPWNVRYPVK

DTTIPGPEPDKPYFIPANTPVSYSVHCMHRRTDYWGPDAEAFDPDRFLDARVQRYLTP

NPFIFLPFNAGPRICLGQQFAYNEMSFFVIRLLQHFDEVQLCEDALAPDCRVPDAWRG

APGRKGVERFWAKAHLTLYAKGGLWVKMREAPTSEAV

 

>CYP63A4 PC-4(pc.151.16.1) Phanerochaete chrysosporium

MALPPGLQYLLPQLPLLLAPPAAVLLAAHAARAFAGTAAPAWALALACVLSWPVALTALVQLRAHRVAREAAARGARLPPAVEARYPGGVDLMRRNNSEVEEHIPGYRLSEFGRQYGWTYNFRMLFQDRVRGRPRPRPPGRILATDFTSYEKGAVFSAQMKSLLGTGVFNADGDLWKFHRAMTRPFFSRDRISHFDVFDRHAEDALKLAKARLSEGVPIDWQDLVSRFTLDSATEFLFGQDVRSLSAPLPHPPTAPQAQHDTHDAEHPANRFAHAFLQAQLASARRSRYTAAWPLWEFWENKVEKHTRVMDEFIQPLLRDALARKAKGADAQAEEAVADGETLLEHLVKLTDDPQIIHDETLNILLAGRDTTAITLTMAGYMLAEHPDILQRLRKEILDTVGTRRPTYDDIRDMKYLRAFINEVLRMYPPVPFNVRFSTAPTVWPSPEGDFYVPAGTRCMYSVFVMHRRKDLWGPDADKFDPDRFLDERLGKYLTPNPFIFLPFNAGPRICLGQQFAYNETSFMLIRLLQRVSKIELHPEVSPQSVAPPGWAASSISDGKDKVVFKSHLTMYVQGGLWVTMQFENPEEH

 

>CYP63B1 PC-7(genscan.57.18.1) (genewise.57.16.1) Phanerochaete chrysosporium

MPHPFSRYRLRVFGDFVRIVLAPSFVFWSAVQILKLRLGLLSPAAWLTFLFAASYARVQYRGFLQRQEARRRGGVLPPEVVGRWPGNIDILIKLGKASLTAYPGSFYLDLFEEYQSTTLNLKLLWSDLVRCLSFCRLSAVLKTLSQIITMDEEHIKHILTTGFNHFWRGRRQKERMYAPSGASRRHDTDSQGDVSQEWKKHRALARPFFARDRISDFDLFEKYAGATLGILGGLAGRGAAVDVQDLYARFTLDAAAEFLFGERLDTLHGALPVAGQAKLGSKGAATDDAFGAFVRAFEASQDIITTRQVRGYFWPVRELFQDKVAPHAAVIGAFLEPIVQRTLDRKAKMRAAGVSPTTEHDTFLDYLADHTEDPKVIRDQLLNILMAGRDTTACLLTYVTYVMAMYPDIMQKMRQEVLHVCGHDAPNFEKLKALRYVHAVLNETLRVFPPVPMNVREVRARGVVLPHADPTYAAAPAPLYVPGGTVVMYLPVLTQRNTALWGDDADVFDPDRWLDARLRRFTENPMMYTPFSGGPRICIGQNYARNEATYLLVRLLQQFDAVALAPEAQPAGSLPPPEWRHARGRAAEERIWPAYAITLYVKVRLSLQWLYC

 
>CYP63C1 PC-5(pc.101.32.1) Phanerochaete chrysosporium

MELHPRQYRLRFLLDVLRAIVWPQLVFNAALYLAGFHPGAFLRVVASVLAVPLLGTVRTAISQRRNKIQAGAALGAKEVPCVRGKWPGNLDIVLGFVRSLKEAYLMQFLDDLFREYDCKTLNMRLLWEDQIWTIDEAHVRYMLAGPGFEWFHKGYYWQERMESFLGNGIFNRWAQRAIARPWFVKDRISDLNIFDRHTTTTLALISEFVDRREAFDAQDLFARFTLDSASEFLFGRCLDTLHGTLPVAGRAKMGPKGTAIEDAFGSFARAFEDVQVQIARRTRIGKPWPLFELFTDKTAPSVAVIHDWLRPIVHEALAKKSAASAEKESGEDSTFLSHLANSTDDPQDIAYSVLNMLLAGRDTTASVLSFVVYFLALHPHVTEKLRAEILQAYGPDGRPSVEDMKDLKYVRAVLNETMRLFPPVPMNLRLSDAHPRIFPASGSAPKYYVAPRTVILYSIFLVQRRTDLWGADALEFRPERWLEPATARLLADHPFAFTPFHAGPRLCLGQNFAYNEMTFFIVRLLQRVSGFELAPDAQPEGSLPPARWKYGEGRQAVEKIWPASSVTTFIKVSLASMPCCGERWLKRRRQGGLWVRAVPA

 

>CYP63C2 PC-6(pc.101.28.1) Phanerochaete chrysosporium

QRAIARPWFAKDRISDLNIFDRHTSTTLALIADFADRREAFDAQDLFARFTLDSASEFLFGKCAETLHGTLPVAGRAKLGPKGSSVEDEFGSFAWAFEELFHDKTAKHRKVIQDWLQPIVREALHSKAAAARGEDTGEGTFLSHLTKTTDDPQDIAYSILNMLLAGRDTTAAALSFTVYLLALHPEVVEKLRAEVVQAYGSDGRPSVEDMKSLKYLRAVLNETMRLFPPVPLNIRTSDDTPRVFPASAGAPKYYVPPRTPVVYSSVIIQRRKDLWGADALDFRPERWLEPETARRLAENPFMFMPFHAGPRLCLGQNFAYNEMSFFVVRLLQRVAALELAPDAQPEGSLPPARWKNGEGRQAVEKIWPGSSVTTYIKVSSTRSRPCG

 

>CYP64A1 Aspergillus flavus U81806

99% to CYP64A1 Aspergillus oryzae

AFL2G_07214 revised

MIYSIIICAGALLGLWILEKLLAPKDTRPPLPPGPWRKPIIGNL

TDFPPKGTPEWLFWAKHQERYGPMSSLEVMGQTIIMINDAQLGIEIMHKKSALSQMIP

DAPFAHMAGWGMSLATERNRQAWKTIRANMKQEIGTRRAISTFHPKMEIGIRRFLLRT

LDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHIFSPGEWSV

NFFPILRYVPSWFPGASFQIKAAEYKRTIERMTMVPYLWIKDQVARGCSRPSILLRLL

QKGHYESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSFFVAMALYPEVQRKAREELDRV

VGPTTLATFEHRSQLPFIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLL

PNIWTFTHDPSVYHDPMVFKPERFLEGKDSPPETDPMKFVFGFGRRICPGRFVTDEKL

FLIACHAVSCFFISPKDPGAPEPDWLPGVISQPGAFDLNVVPRSPAHEELIRSIETDH

PWKNADATDISRFMARNQMI

 

>CYP64A1 Aspergillus parasiticus AF017151

 

MIYSIIICAGALLGFLILQKLLAPKDTRPPLPPGPWRKPIIGNL

TDFPPKGTPEWLFWAKHHERYGPMSSLEVMGQTIIMINDAHLGIEIMHKKSALSQMIP

DAPFAHMAGWGMSLATERNKQAWKTIRANMKQEIGTRRAIATFHPKMEIGIRRFLLRT

LDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHIFSPGEWSV

NFFPILRYVPSWFPGASFQIKAAEYKRTIERMTMVPYLWIKDQVARGCTRPSILLRLL

QKGHYESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSFFVAMALYPEVQHQAREELDRV

VGPTTLATFEHRSQLPFIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLL

PNIWTFTHDPSVYHDPMVFKPERFLERQSSPPETDPMKFVFGFGRRICPGRFVTDEKL

FLIACHAISCFLISPKDPGAPEPDWLPGVISQPGPFDLNVVPRSPAHEELIRSIETDH

PWKNADATDISQFMARNQMI

 

>CYP64A1  Aspergillus oryzae

         GenEMBL BAE71330.1, BAE59527.1

         99% to CYP64A1 Aspergillus flavus

MIYSIIICAGALLGLWILEKLLAPKDTRPPLPPGPWRKPIIGNLTDFPPKGTPEWLFWAKHQERYGPMSS

LEVMGQTIIMINDAQLGIEIMHKKSALSQMIPDAPFAHMAGWGMSLATERNRQAWKTIRANMKQEIGTRR

AISTFHSKMEIGIRRFLLRTLDNPDDLRFHIRKEANAFMMDVAYGYTIAPHGKDELYDLTQQSVRQFSHI

FSPGEWSVNFFPILRYVPSWFPGASFQIKAAEYKWTIERMTMVPYLWIKDQVARGCSRPSILLRLLQKGH

YESGSHQEQVLVWTNAEFVMGGSDTTVSAVSSLFVAMALYPEVQRKAREELDRVVGPTTLATFEHRSQLP

FIDALVKEVFRWHPASPLGAPHITQEDQIWDGYLLPKGALLLPNIWTFTHDPSVYHDPMVFKPERFLEGK

DSPPETDPMKFVFGFGRRICPGRFVTDEKLFLIACHAVSCFFISPKDPGAPEPDWLPGVISQPGTFDLNV

VPRSPAHEELIRSIETDHPWKNADATDISRFMARNQMI

 

>CYP64A1 Aspergillus nomius

AY510454

join(63157..63352,63429..63558,63614..63805,63870..64021,

64071..64315,64373..64644,64705..65104)

gene OrdA of aflatoxin biosynthesis

93% to CYP64A1 Aspergillus flavus
MIIGLIICAGALLGLWLLEKLLAPKDTHPPLPPGPWRKPIIGNL

TDFPPKGTPEWLFWAKHQGRYGPISSLEVMGQTIIMINDAQLGIEIMHKKSALSQMIP

DAPFAHMAGWGMSLATERNKQAWKTIRANMKQEIGTRRAISTFHLKMEIGIRRFLLRT

LDSPDDLRFNIRKEANAFMMDVAYGYTIAPHGKDELYDLTQRSVRQFSHIFSPGEWSV

NFFPILRYVPSWFPGASFQVKAAEYKRTIEKMTMVPYLWIKDQVARDCCRPSVLLRLL

QKGHYESGSQEEQVLVWTNAEFVMGGSDTTVSAVSSFFVAMALYPEVQRKAREELDRV

VGPTTLATFEHRSQLPFIDALVKEAFRWHPASPLGAPHITQEDQIWDGYLLPKGALLL

PNIWAFTHDPSVYQDPMVFKPERFLEGNGSPPETDPMKFVFGFGRRICPGRFLTDEKL

FLLACHTLSCFLVSPKDPAAPEPDWLPGVISQPSPFGLNVVPRSPAHEALIRSIETDH

PWKNADATDISRFMARNEMI*

 

>CYP65A1 Fusarium sporotrichioides AF011355

MFQYSLWPLLALSGGTGLAYLVVVVVYNLFFHPLRNFPGPWLNS

ITQVPHTLLMLCGLPHKKHLALHMKYGPVVRIGPNMLSFNHPDAMKDVRGHRKSGEPE

HGKDPISVQSNGDNIVGSDRENHTRFRRALAYGFSAQAMLEQEPTFKAYVNQLFQRLH

EQSSGGTKPVDISKWYTFTTFDMIGDLAFGESFSCLDNSTYHPWVSLAFESLKSLAFL

AEIGRYPRIAPYLGLLVPRGLLTKFAENKELASMKVRKRLDTETDRPDFVGKITQGLK

SKGTSMEFNELASNASVLIVAGSETTATLLSAAVYFLCAHPRTLDLLTKEVRSTYTQA

HDIDLVSTQGLRYMQAVLDEALRMYPPVAGGGSPRKIAKGGSFVAGHFVPENTLVEND

MWAMHYDPKYFTQPHDFIPERWLGDVRFANDRLDAVKPFSIGPRNCIGMNLAYAEMRM

MLARTVWEFDIRLSEGSRNWYEESRVYLAWNKPPLNVYLIPR

 

>CYP65A2 Fusarium graminearum FG03540.1 AACM01000159 FGcontig1.159_scaffold2

MFQYSLWPLLALSGGTAVAYFLVMLVYNLFFHPLRNYPGPWLNTMTQIPH

TLLMLCGLPHKRHLALHMKYGPVVRIGPNMLSFNHPDAMKDVRGHRKSGE

AEHGKDPIIVLSNGDNIVGSDRENHTRFRRALAYGFSAQAMLEQEPTFKA

YVNQLFQRLHEQSSSGIKPVDISKWYTFTTFDMIGDLAFGESFGCLDNST

YHPWVALAFESLKSLAFMAEMGRYPRIAPYIGFLLPRGLLTKFAENKELA

SMKVRKRLDTETDRPDFVGKITQGLKAKGSRMEFNELASNASVLIVAGSE

TTATLLSAAVYFLCSNPRTLELLTQEVRSTYTQADAIDLVSTQGLRYMQA

VLDEALRMYPPVAGGGSPRKIAKGGSFVAGYFVPEDTLVENDMWAMHYDP

KYFTRPNDFIPERWLGDARFSSDRLDAVKPFSIGPRNCIGMNLAYAEMRM

MLARTVWEFDIRLAESSRDWYQDSRVYLAWNKPPLNVYLDPR*

 

>CYP65B1 N. crassa AABX01000364.1 cont3.489

23181 MMMMMMNLSHLLPLGIPLPSPTSLIGIFLIG (0) 23273

23368 TSILVSSILLSAIYNLTLH

      PLARHPGPFFHRASILPYLYRQITGTLPCSILDFHARYGPVVRI

      SPNALSFADPQAWKDIYGHRPHGEEEFAKLNLFYRIKGSPPSLL

      SETKEAHGTLRKLMAHGFSDRSMRAQEGIIGGYVSALIRGLRGNC (1) 23823

23968 QEQDERVPLDMVSWYNWTT

      FDIIGDLAFGEPFGCLEKAEYDPWVDAVGKSVRFGCVMFALRLL

      GLEDWVCPLVRKLSGNARRFHRKRTMDKLQRRVKLTKERPDFLE

      GLLQKREEW (0) 24315

24423 GIDMDALAANASLLIVAGSETTATLLAGATYMLLRDPEAMKKLT

      EEVRSTFKSEEEITLSSVGNLEYMLACLNEAMRLYPPVPIGMPR

      VVPKGGAKVAGTFVPEG (0) 24737

24819 TVVAVWHWATSHNEQHFVEPFEFHPERWMQDPRFANDRLDAVQP

      FSVGPRNCIGRS (2) 24983

25076 LAIAEMRLILTKVVYNFDMKLANPEKDWLDQHIYTLWDKHPLP

      VYLTPIERNGV* 25240

 

>CYP65B2 Magnaporthe grisea MG06544.4  55% to 65B1 new N-term AACU01000188 cont2.1218

MIPPYIEPNVLGLVKLTAVL

 (0) LPIYLLGHAVYNLFFHPLRRFPGPCLQHISYHAYAYR

LSRGTLAHDVAKWHRQYGPVVRISPDQLSFIEPDAWKDICGH

RTGSKANVDEMPKWPGFYTVNGRPRTIINETKENHAVLRRSLAHGFSDRS

MRLQEPIIGSYVDLLIRRLKERCHSEPAADRPGGLTTRQDIKTWYNWTTF

DVIGDLAFGEPFDCLEKAEYHPWVDNMNKTTKSFGAFMALKLVGFHWLVT

AILKYGLSKTREDHMRRTMAKIERRASLTVERPDLIEGLLQLKGLGKEQI

RITAGTLIIAGSETTATLLSGVTYLLLKNPEKLARLNEEVRSAFNDDNEI

TLMSVANLPYMLACLDEAARLYPPVAGGTPRVVPAGGASIAGTYVPENTV

VSVWQMAAYQNEQNFHEPFSFKPERFIERNSKVNTRDRFEVLQPFSVGPR

NCIGRNLAYAEMRLILAKILFNFDIELADKEKDWIDHKSYVLWDKPALDI

YLTPVRP*

 

>CYP65C1 N. crassa AABX01000034.1 cont3.25

20288 MPVIDKLAGVTGLSGLHLLLGAVGVT (0) 20365

20487 ILLYIIGSAIYNVFFHPLASYPGPLLQRASPIPFSIRHALGVQP

      FHTQALHDKYGPVVRIAPNHLSFTDVRAWRDIFGHIAGSRHGQE

      ELAKFKSFVRPIDAQPIHIISAGREKHGQLRRALANSFSDASMR

      GQGPLIGKYIDLLLHKLHEQGQEGKVPLNATSWYNSVTFDVAGD

      LIFGMSFGALDQNGNHSWLQYILGSLSSLAPMAALSYAGLHWLV

      QLLWRAKGAEVFRKSMESVDLMLKERLKMPVDRNDLFEGLVQRQ

      EKL (0) 21287

21563 GLSFEELASNAWLLVL (2) 21610

21675 AGADTTATTLSGTTYLLTQHPEVLKRVTQEVRSSFKSVEEIDIS

      SVNRLTYMLAVLNEAMRLYPPVTSLTARIVPRGGAHVLGEYLPEG (0) 21941

22014 TIVDIQQWSMNHSKENWVDPWAFNPARFLDDKETAREKGNVLDAL

      QTFSVGPRNCIGRN (2) 22190

22266 LAYAEMRWILARILFDFDLRGAPTNQNWIERQKAYGLWDRIPLD

      VYFEPARERK* 22430

 

>CYP65D1 Magnaporthe grisea MG03832.4  47% to 65B1 partial seq. length 53916

runs off end, changed C-term AACU01001030.1 cont2.747

N-term on AACU01001031.1 cont2.748

seems to be missing some N-terminal seq.

    MFSVLLQLLNDPLAGIPGPIYCRFSRLPYVYWVCTGRLVFRIKELHDQYGEVFRIAPDEV 289

288 QFINPEAWKDIFGHKTQGAKEFPKDLRFYPMPPGGVRDLLTADQANHPRMRKMLSPAFAE 109

108 KTLREQEAIITSYVDFMVGKLRGMVTKQED 19

sequence gap here

53789 RPDFMSYIMKGAETGQPLS

KEELDANADLLVFAGSETTSTFLSGCTYFVLSNPRVYRRLVAEVRGSFNS

EEEMTFASIQRLPYLSAVMQEAFRMYPPIPVGPPRKVPEGGAVVRGISLPEN () 53427

53301 TAVAITQYAAGHSETNFTDPYTYTPERWLPDADERFASDRRRAVQPFA

VGPRNCIGQN () 53128

53026 LATAEIRLTMIKMLWHFEAELAPECENWHKQSIFGLWEKPPLMIKLRPRER* 52871

 

>CYP65E1 Magnaporthe grisea MG03830.4  37% to 60A3 AACU01001030 cont2.747

MAAPMSAFTIGNLFMLLVVALGI (0)

ISIYYGLLCIYNLYFHPLAKYPGPRLA

AATHLWLMRAYLSGKAHRHIHEAHKRYGPVVRTTPDGLSYVDARQWKEIY

GHQPSGRPEFSKQAFASAVFEEPTLHNADREYHGYVRRLFAHGFSEKALK

GQESVLKEYVDWMFAGIERESRGGSRPVNVVNWYNYLTFDFIGFLTYGES

FDCLRTSSLHDWIKLFISFGKLLIYGQVLLPIPRLLKIPAALLLIPSKLR

EDRKRASALQSEKIKHRLSIQPTITDFTTKMVEAYNNGKMSMNQLEGNSI

LLIGAGSETTATALSGLTWLLAQNPAVLAKLAAEVRSAFASTEQITSTGV

NSCKYLLACVEEALRVYPPLAQPHYRIVPRGGASVGGDHLPAGVVVSVPK

YAAARSPLNFHRPDDFCPERWLGGHDGEFAGDQRDASQPFSVGPRACIAR

NLAYLEVRLVIARLVWQYDIENATEGDWLGGQNLWHLWEKGPLWLRLKAV

R*

 

>CYP65F1 Magnaporthe grisea MG08498.4  42% to 65A1 AACU01001266 cont2.1600

MGLMQQTPAASFGLGTSTLLFSLVGFGIVSYVVYLAVVRPIYNVFFHPLS

KYPGPKSWAASRIPYVLSSTGGHLQYKLLELHCKYGDVVRIAPNELSYVR

PDAWKQVMGHRKHGESENGKERVNYTSFADSIIGADRHEHAQGRRVLSHA

FSAKRMLEQEPLIKKYIDLFFERLPVISEGCSKPVDIVRWYNYTTFDIIG

DLTFGESFHCLESKTMHPWVRLIFASVRDGNLLSNMRAYYSNIDSFMYRF

FPSAVKHKMQHEEMLRQKLKQREAMGSSRPDFYDSMTRRDKNGFGFTPIQ

IERNSDLLVGAGSETTATVLSFSTYLLCKHPEIQAKAAKEVRELFKSEDE

ITMTQVQQSHYLLAVLNEAMRLWPAAPAELPRQTPPEGSMVCGEFIPGDT

ILLVAQYPSNHYPGNWVKPEEFIPERWLKDADPIYDADKKEAFNPFNHGG

RDCIGKNLAYSEMRMILARMLYNYDMELDEQSKNWAEGMLAYTLWDKPPM

FVHLKPRKTEA*

 

>CYP65G1 Magnaporthe grisea MG00023.4 39% to CYP65A1 41% to 65B1 AACU01001352 cont2.7

MSRLDLIHDLRSFAFGQPFWHYVWFALFLTTCYYLATTVYCVYFHPLAKY

PGPRLYAASQIPYAVVFAFGDGHNTIARLHREYGKVVRIAPDRLSFSSPD

SWNEIMGHQKKDRNREHGKTPNFYDPLSIPGTDREAHARQRRILAVGFSA

SYIVEQESIIHYYVGLLMNRLKENGQDGTKPLNMVNWFNWTTFDIVGDLA

FGESFGCLETGVYHPWVSLMFTHVKLGAWNYAIAQFPTFSKIIRSFVPKS

MTKKVEEHHAYTRDLVDRRLETDSSRLDLFRAMAHPRDGLSMSKEEIYSN

TGTLVEAGSETTSTALSGVLFHLLKSPEAMKKVKDEIRGTFKSEDEITYV

SVQNLEYLSACVREGLRIYPPIPSTVPRITPKDGSIIFGEWVPPDTKLDI

WQFPLSRNEKFFKDADKYVPERWLGDPRYQSDCREASQPFSVGPRDCLGK

NIANGEMRIILSRLLWNFDVRLADDSVDWDQGQKVYIIWLKSPLNVYLTP

VKRS*

 

>CYP65H1 Magnaporthe grisea MG10954.4  39% to 65B1 40% to 65A1 41% to MG05390.4

AACU01001824.1 cont2.2124

MLEKYALRAIRPSHLFTYS (0)

AVAFLLGKCIYNLFFHPLRHYPGPFLCRASRVFYLFWD

VRGLSHWKVKQWHDQYGEVVRISPDELSMTNPEVWSTAYGFGAGGFDKDP

KWWNKNVGGVDNILTADDQGHRRMRRLQNPAFSDKALRSQEPRMTRYIDM

LIQQLRRQSSQGPTVDINLWYNFATFDLIGDLAFGEPFHCLRDGRWHWWL

TAVFEIFKAGTYIRAARRFPTALYYAILLCIVPKKLLQMRERQYMFGVER

INRRLEQTDTDKTDFMSYILQADDEKGMTVEEMYTSAQVLIVAGSETTAT

ALTSGTYFVLSNPDVYARLRAELDAAFASEADITLQSTQRSHLPYLHAVI

DETLRMFPPGPGAFPRVVPDGGRTILGRFIPGGMTVGMHHLSVGRSRENY

VLPNEFHPERWLAEDKRFDLDRKAASQAFSFGPRNCIGKNLALAELRLIL

ARMFWNFDMELCPESKGWTDRQQMYTTWKKTEMPIKISPRVR*

 

>CYP65J1 Magnaporthe grisea MG10070.4  39% to 65B1 , 40% to 60A2 AACU01001328 cont2.1928

MSATFKLDPASLDSLLINLPPLNWENGLRGISIALLVYFFILKPFYNVYL

HPLSSYPGPKLWAISRIPWHYTNLKGRISWRIREMHDTYGPVVRIAPDEL

SYTSSTAWRKIYAQRTPEFMKALDGRGIAPTSIGGRRSLMTETQDNHLRL

RKAINPAFSERALREQEHYLQSHSDNFVKVLTERSARGPVDMTTWYNLLA

FDIVSDLAFGEPAGFLDNADQPWIQVILHRAKAISWYQLAVWYGFFPLLE

YITPRYVTEARKNHIRLTEAKLERRMAATNPGRDFMSFILDNDNEAVTKL

SKVELVMLSSNFIVAGSGTSAGGMSGLTYLLLRNPDKLERLRREIRSTFA

RNQDITMVATAKCRYLNACLDEGMRLYPPTPGSLPRIVPGDGQEIDGRWV

KGGMGVAVNQLSAGHSELNFKRAREFHPERWLHLEPGSEFANDDRAASQP

FSAGQRVCIGRSMAYAEMRLTMAKFIFHFDMELANPEMDWWNQQGTYLVW

EKIPLMIQLHRRT*

 

>CYP65J2 Alternaria solani

Alt3 BAD83682 69% to 65J1 Mg

PKS for alternapyrone is next to three P450s called alt1, 2, 3

Chem Biol. 2005 Dec;12(12):1301-9.

        1 malssaldsl whqldqllsl inrniitgii vlpvlyvllk viynlylspl agypgpklwa

       61 vsrlpwnran mkgriswkir elhdkygpvv riapdelsyt tsgawkkiyg qrnpefvkal

      121 dgrgiapasi ggqrslmteh qdkhlrlrra idpafsqral reqesyfqdh sdnlvqklkq

      181 rckdgpldmt twynlvafdi vsdlafgeps gcvnnpdqpw iqailaraka ivwfqlavqy

      241 gfmgllnwmt pkyvtesrkk hiamteaklk arveaknpgk dfmsyilend eklnhlelvm

      301 lssnfivags gtsaggmsgl tylllrnpdk leklkqeirg lfksradmtl qavtsckylr

      361 aclnegmrly pptpgslprf vpgkgemieg kwvpggyavg vnqlaaghse rnfkkarefh

      421 perwldepds efkdddrsav qpfsygqrgc igrsmayaem sltmaklvwy fdweldepdn

      481 dwwnqqgtyl vweklplqvk ltpvsdvve

 

>CYP65K1 Magnaporthe grisea MG09945.4  poor match 41% to MG02982.4

AACU01001747.1 cont2.1897

MDYISNA (0)

AVYDLTIHPLARFPGPRLSALSRLPFCIARMRGDQVQWMHSMH

LKYGPVVRFGPDELSYVDQGGSAWKAIYGQEKGAKEFPRLKAWFRAAFSP

AFSERALRSQEPLFRRQIDLLISILASDAAETGGPANLTELFEFTTFDIM

GDLALGEPLGMLQTQQYSKWMKALASGVRIMPVFQFIQHYPLVSKIFSLL

EPEWVTQLKYEHHQYNVDRVNARLARESSSQADIWSYVLSTKEKQRLTVE

EMHSNAESFMIAGSETTATAMTALTYFLTTSPSRFSRLKNEIRSRFSSEE

DITMETTSPLKYLNACIQEALRMYPPLPVGVPREVTSPGATILGRWVPGG

TQVSVHHYASSHSPGNFTDPDTFAPERWLAREPADGDNGTNATDSARYGS

DRLEATQPFTVGPRNCLGKSMAMHEMRLMLACLYFRFDMELVSDAGAVPW

NQQKAFAFWDKKPLMCKIKLANGGI*

 

>CYP65L1 Magnaporthe grisea MG02982.4  42% to CYP65B1 38% to 65A1 AACU01001589 cont2.600

MASNGAGVFYSSAFFQIRDLGAAEIVRRIALFSAYSFSLFLVFFTIRAIY

NIWFHPLSKYPGPWYAATT NLVYYIADITGYRERWILRQHQKYGEVVRTG

PIRLSYTSASAWKDIVGHRSAGKLEMLKDPWLYDAGQAFGSDGVSMISNV

DSETHGKMRKIFTSAFSDRALREQEPLLLKYVDKLIRRIHTDLSGDPNRP

LDLVLLLNCTTF DIMADLTFGESLGMLDNGKYTPWVARIFDTIRYAAIFG

IAGRWVWLRNLVRATLPASMRKSLEEHRGYVNER VERRMDKDTDQPDIWN

LVLRNPENVKLLGRGSMNEHANLFMVAGTETTATLLSGLTYFLLKKPSTY

EKIVAEVRSLPEDGLHIDSLRQLPYLQACFEEALRMYPPVPMGMPRVVPP

GGAAISGGFVPEGTRVAVHQLSCYRNEKNFKNAFEFIPERWLPEYADEYG

SDRKEALQPFSYGPRNCMGKNLAYHEMRLIFAKLLWNFDIELCPESDNWT

DQRIFILWEKHPLMVKFKPRKV*

 

>CYP65M1 Magnaporthe grisea MG00651.4  37% to 65B1 AACU01001445 cont2.120

MVALVRGSVVNLGFW

GWILITGVMLLAIPTYIFIYNIWFHPLAAFPGPLMYRAFDSFKVYQQLTG

NINHRLHAAHEKYGDVVRIAPWQLSYTTATAWTDITAGHKGQIPTNPAYG

QAEKDLFGASGFLWVGNDEHARHRRVLSPAFSDRSLREQEPVVSKYLDLL

VARMKERSGDVVDFWLWMNFTTFDIIGDLTFGESFDCLLESRLHPWVDYL

MSRLHMMMYGQIMSTMGNLGVLVEMMVPKRIMDEAKRHVSLAKDKVNRRI

ARTTDRPDFMTQILAHTTIDTEAAGESKHKNRLSLPELYADTQIMVIAGS

ETSAGLITAAAYFLLRNPKAMGRLVREIRGTFAQETDMGNAAVSKLPYLN

AVVNESLRMRPPLPSGIDRKVGAGGAVVDGKFLAPGTSLQVTHWAAYHSA

RNFAEPWSFRPERWLSEREAEEDVSVFAGDNKAVFQPFGVGSRGCLGRGL

AYMETRLTLARLVWNFDMELMADSADWADQRVWMLYEKKPLNLRLVPRGM

AESYELSAQTRDRLWHTAGESKVDVRG*

 

>CYP65N1X # name changed to 563B1

no sequence

 

>CYP65P1 Magnaporthe grisea MG07983.4  48% to 65B1 partial RUNS OFF END

AACU01000985 cont2.1482 C-TERM HALF

MSEGEIISNSALLIIAGSETSASYLAGVTYHLCTYPYVMKKLTSLIRSSF

ATEAEITSSSLAQLEYLTAVLKEAGRTYPALPSGQPRLVTETGAFISGEM

VPPGTLVFSHPMGASMAPFNFTNPEQFVPERWLKDPPFPYNDDNLDASMP

FGLGPRSCIGQSLAWIEMRLILGRLLWNFDLSLSPECSRWIKEQKVFQVW

EKTPLKVKITMRHDLERK*

 

>CYP65Q1 Fusarium graminearum FG07765.1 AACM01000318 FGcontig1.318_scaffold4

MSVLSILLDGEVIHPMVLLPLAAVTMLVGYVLFWVIYNLSFHPLSKFPGP

RLWAISTIPYIRNFTAGECHFTILEMHKKYGPIVRVGPNDLSLNHPDGMK

ALRGHRKSGTGENSKEPVAASFNADNVIGANRENHQRYRRTLAHGFSQHS

MMAQQPIIRVYVDKLMKGLHAASDNGTKPVDIATWYNFTTFDVIGDLAFG

EPFGCLDTGKLHPWITLIFAGMKDLAFITTMARTPWLNNLWNLVTPKKST

SEWASHVEMAREKVRKRLASGMDRPDFIDAMLKRTGAAGNEMTFEELASN

AQILMIAGSETTATVLTATTYFLASNPETLKKVVNEVRSGFSSENDINMD

SVKKLTYMLACLNEGMRVFPPVINGSVRTIRPEGDDIIGHYIPGGATVDV

WQWAVHNNPDHFAHADQYIPERWLDNNDPRFANDAKQALNPFSIGPRDCV

GKNLAYAEMRMILARVLWNFDVSLHPDTPGWEKRTKSYFLWERGAMNVML

TPRKTE*

 

>CYP65R1 Fusarium graminearum FG03191.1 AACM01000148 FGcontig1.148_scaffold2

MFSALTALAGSLVLYIICTSIYYITFHPLASIPGPKICGITRIPYWLVAF

RGEDVRWMKTLHDKYGPVVRFGPTDVSYAATQAWNDIHGPKDSEKAQEFS

VQPVNGVPSMLTTDVENHTRMRRLFSPAFSERALKKQEPLFKKYSELLMY

KISEVGDNGAKPVEMCQLLNFTTFDVMAELCFGDHLDLLAKNEYSPWVRS

IFESLKMLPIASMINYYPILNALFTRFEPK SVTQQRVTHCKHSEERVNRR

LENGSDQPDVWNLVLEAKEGKGITVK EMHSNAELFMLAGSETTATLLSGC

LYYLLSCPDKMDILLKEIRSKFDKVDDITFERLAELKYMNVCIKEALRIY

PPVPIGSPRVVSPGGQQILGKYIPAETRVSVHHWSTYRSESNFKDADKFV

PERWLKTEARYAGDALEAHQPFGFGPRNCLGQNMAMHEMRLILTTLVFSF

DFELCEESKNWADQKSFALWIKNPLMIRATPVTTHARLNI*

 

>CYP65R2 Fusarium oxysporum

82% to CYP65R1

frameshift at TLQ/KMH = &

FOXG_10066

MGPILASIAILALYLLGNAIYHATLHPLANIPGPKICGITRIPYWLASLKGEDVKWMKGLHDRYGPVVRFGPTDVSYTAS

EAWNDIHGPKVTEKAQEFSVQPVNGVPSMLTTNTENHTRVRRLFSPAFSERALKQQAPLFKKYSELLMYKISEVGNDGAK

PVEMTQLLNFTTFDVMAELCFGHPLNLLVHNEYSPWVRSIFESLKMLPIASMINYYPILNAIFTRFEPKSVTQQRVTHCKHSEDRVNHRLENGSDQPDVWNLVMSAKEGKGLTLQ &

KMHSNAELFML

AGSETTATLLSGCLYNLLTYPEKMKTLLEEIRGKFTNTDDLTFERLAELKYMNACLKEALRVYPPVPIGSPRVVQPGGQQ

ILGKYIPPETRVSVHHYSTYHSESNFRDADTFVPERWLKTDSKYAGDMWEAHQPFGFGPRNCLGQNMAMHEMRLILATLL

FKYDLELCEESRNWVDQKSFALWIKTPLMMRAKPVATHTRLNI*

 

>CYP65R2 Fusarium verticillioides

95% to CYP65R2 Fusarium oxysporum = ortholog

FVEG_08438

MGPILVFIAILGLYLVGNAIYYVTLHPLANIPGPKICGITRIPYWL

ASLKGEDVKWMKGLHDRYGPVVRFGPTDVSYTAGEAWNDIHGPKVTEKAQEFSV

QPVNGVPSMLTTDTESHTRVRRLFSPAFSERALKQQEPLFKKYSDLLM

YKISEVGNDGAKPVEMTQLLNFTTFDVMAELCFGHPLNLLAQNEYSPWVRSI

FESLKMLPIASMINYYPILNAIFTRFEPKSVTQQRVTHCKHSEDRVNHRL

ENGSDQPDVWNLVMSAKEGKGLTLEEMHSNAELFMLAGSETTATLLSGCL

YNLLTYPEKMKTLLQEIRGKFTSTDDLTFERLAELKYMNACLKEALRVYPPV

PIGSPRVVQPGGQQILGKYIPPETRVSVHHYSTYHSESNFKDAESFVP

ERWLKTDSKYAGDMWEAHQPFGFGPRNCLGQNMAMHEMRLILATLLFKFDLE

LCEESRNWADQKSFALWIKTPLMMRAKPVATHTRLNI*

 

>CYP65S1 Fusarium graminearum FG08023.1 AACM01000323 FGcontig1.323_scaffold5

possible deletion at the I-helix

MAIISTDLASGTSLGGGLVLLAAAVVLFVTGRAIYYVFFHPLAKIPGPKL

YAATQLPYLYHLTRGQWVYRLKELHEQYGPVVRYTYNDVSFITADAYKTI

YGHKTGGAREFGKDFRLYRQGRPAPSLIVTKHEDHKRQRKLLSHAFSHKA

LSEQNDLLMHYVNLFIEKLTQKAREGESVDMVAWYNFATFDLIGHLALGQ

PFGCLESGKYHPWVTKIFSSIKGISFSQAAIRLGLHNWISLITPAHVKKA

TKEHFEFTEHVAGARLDVKDNNSKDFMSYILRYNDER

XXXXXXXXXXXXXXXXAGSETTATLLSGV

TYQLLTNPEAYKKLVTEIRLAFATEEDITAAAVDKQTYLLAVLSEGFRMY

PPVPGALGRTIPKGGDFVEGHWFPENVGVSVPQLPAYHSARNFKDPEKFV

PERWMDDPRYSNDCRAVVQPFSMGPRDCIGKNLAYLEMRLLLTRLLWKFD

AELMPESREWKEQKIFTLWEKGSLHVKLTEVVREKN*

 

>CYP65S2 Nectria haematococca

e_gw1.7.949.1 short on N-term

Necha1/scaffold_7:62397-63948

54% to 65S1 F. gram. yellow indicates 65S1 is short there, needs correction

MAVLSVFSSQLASFTAVAVALSLT(0)

VVLYLSLRGAYYAFFHPLAKVPGPKLYAASSLPYYLHLLRGDWHQSLKEMHDRYGPVVRFAPADISFITATALKQIYGHK

SSLDATFVKDPPFYHRGMKDPTIINANHEDHKRFRRLLSHAFSEKALRSQEDILKLYIDKFINRLAQKAH

DGVAVDLVSWYNFATFDLIGDLSFGEPFGCLDSGGYHPWVRLVFQHVKAIPYRQIAQYLNMGFLVPYLTP

KRLVKASKQHLQLSRETAMKRVATTTDRADFISYILRHNDEKG MSDGEIAANSHILILAGSETTATLLSG

VTFQLLKNKAKLDKLISEIRSSFDAEDEITLERVNQLSYLLAVLNEGLRI (1)

YPPVPTGLPRLVPSGGEIMERFWIPEKA (1)

ISVPHWAAFHSE SNFRDADNFVPERWLGDISYTNDSKDVLQPFSTGPRNCIGKN (2)

LAYAEMRLILARLLWKFDLEILEDSKDWDSQKSFLLWEKGALLVRLTEVIRD*

 

>CYP65S3 Nectria haematococca

fgenesh1_pg.scaffold_4000060

Necha1/scaffold_4:143263-145075

60% to e_gw1.7.949.1, 56% to 65S1 Fusarium graminearum

MPALSHYFERMDNVPPLGIAILAASLA (0)

VLYFFASGIYYAFFHPLAKVPGPRLYSFTQLPFFYYWIQGNWERRLKELHDKYGPVVRFTHKDVSFITAD

AMKKIYGHKTSMAQTFEKDALFYKPVRDQTDIINANTEDHRRFRRLLAHAFSDRALRGQEEIMQHYVDKF

ILRLTELAHQDTGIDVVRWFNFTTFDIIGDLAFGQSFGCLDSGGYHPWVAMIFSSVKTGPLRAAMQRLGL

GKFVSLLMPAHLKRSLEEHFQLSKSTAMKRIESRDTEREDFMSYILRHNDEKG MTRSEIAENAKILIIAG

SETTATLLSGVTFQLLTNRDKYEKLVKEIRTSFTNEQEITIARVNQLHYLIAAFSEGSRM (1)

YPPVPIGLPRETPKGGEEIEGYQIPED (0)

VTVSVSHWSAYQSEINFRDPKMFIPERWLGDPRYDGDAKEVLQPFSIGPRNCLGKN (2)

LAYAEMRLILAHLLWKFDLELVSESKNWNDQKVFNLWEKGDLKVKLTPVGEIGRD*

 

>CYP65S4 Fusarium oxysporum

58% to CYP65S1 Fusarium graminearum

FOXG_02668

MGVLHTEALAELSLGSAMALSGIALLLYLFISSVYYAFFHPLAKVPGPKLYAITQIPYLYYLSKGDWVFHLAKLHEQYGP

AVRFTPKDVSFITAEASKKIYGHKAAGNKSFEKDFRMYRQNRPCSSIVRSDQEDHRRMRRLLAHAFSSKALRGQGDVVDH

YVELFMKGLTERAQRGEEIDIVAWYNFASFDLIGHLALGQPFGCLENGKYHPWVSRVFKGLKVLSYTQIYVRLGLQNWIS

LLSPPSLRKSYEEHMQFAEHAALARIESKDTDSQDFMSYVLRHNDEKGMSQLEIIENSGVMIIAGSETTATLLSGTTYFL

LKNPDKLEKLINEIRSAFSSEEEITVTQVDQLKYMLAVFNEGFRMYPPVAVGLSRLSPAGGEFIDGYWIPENTAVSMPHW

PAYYSELNFRDPKKFVPERWLDDPRYASDNRAILSPFSLGPRDCIGKNLAYTEMRLLLARMLWKFDLEAVPADKDWYDQK

IFFLWEKGGLPIKLKEVVLKAARFSDLQRATKSNCNASEYHEWAILGSVAKSSSPACIPRTQQWDAHKATAHKPDASSSI

EKSICKTGVNSLVMNFCFTFEFLSNASPRCTITTSEESKDDSEYESKSRASVYR*

 

>CYP65S4 Fusarium verticillioides

94% to CYP65S4 Fusarium oxysporum

FVEG_14141

MGVLYTEALAELSFGSVFALLGISLFLYLVVSSTYHAFFHPLAKVPGPK

LHAITQVPYLYYLSKGDWVFHLAKLHERYGPAVRFTPKDVSFITAEASKKI

YGHKAAGQKSFEKDFRMYRQNRPCSSIVRSDQEDHRRMRRLLAHAFSSKAL

RGQGDVVDHYVELFMKGLTERAQRGEEIDIVAWYNFASFDLIAHLALGQ

PFGCLENGKYHPWVSRVFKGLKVLSYTQIYVRLGLQNWISWLSPPSLRKS

YEEHMQFAEHAALARIESNDTESQDFMSYVLRHNDEKGMSQLEIIENSGV

MIIAGSETTATLLSGTTYFLLKNPDKLEKLTNEIRSAFSSEEEITVTQVDQ

LKYMLAVFNEGFRMYPPVAIGLSRLSPTGGEFIDGYWIPENTAVSMPHW

PAYYSELNFRDPKKFVPERWLDDPRYASDNRAILSPFSLGPRDCIGKNLAY

TEMRLLLARMLWKFDLEAVPTEKNWYDQKIFFLWEKGALPIKLKEVVREKV*

 

>CYP65T1 Aspergillus nidulans AN7522.1 38% to 65A1 53 clan

MPLTLSLILWPLVGFCLYVAIITTWYTLFSPLRVIPGPKSWTFFPILRHI

SAILGRFDTDMRRFHAQYGPVVRFGPREVSFITADAWKTIYGHGHTQLPK

YMHSAADPQSIISTNDTDHSRYRRALSHAFSARGLQEQEPLIQSYVDKLI

VRLKGVAESGRPEDMVKWFNLTTFDIIGDLAFGEPFGGLDSAEYHHWVST

IFQSIKVNPFILLKDNYPMLGWLLLRAFVPETLRQAKKRQIEHSRVTVQK

RLSSDIHTRPDFMDSMLRSRHYTYNQNDWKKAPDALTDSEIVSNSNILII

AGSETTATLLSGVTYWILRNPRVLSRVVCEVRSVMKSEADITVQKVSTEL

PYLLACFDEAFRLYPPVPTGLQRRTLAPTFISGYEMPPGTIVSVHQSGAY

RSESNFHLPNNFIPERWLPDAKTNPSSQFYHDNRAVVQPFSVGPRNCIGR

NLAFAEMRVILARVLWNFDLELCKESENWSDQKSKLWAQPDLATEEAIRL

H*

 

>CYP65T2  Neosartorya fischeri NRRL 181

         AAKE02000011.1 NFIA_002780

         DS027697.1  complement(627606..628748)

         57% to 65T1

MMFVILAAASLLYISYILLLAIKTIYFHPLSHIPGPKWRIGIPLLHHLS

628603  AIRGRLDLDISSWHEKYGDVVRSGPDEVTFITAQAWKDIYGHGHRQLPKVQISAINGKDI  628424

628423  FSANDVDHARFRKALSHAFSAKGLQAQERIVTQYIDKLIQRLKWFAESGTAADMGKW  628253

YNLATFDLIGDLAFGEPFGGLDSA

EYHHWVATMFGFVKSIPFLRALHRYPVVFKVILAFHPGSLMEMRSKQVEHAKATVQKRL

SNSAAGRGDFMDSMLRHQGDKGGLSLDELEENANVLILAGSETTAALLSG

VTYWLLRTPDALDKVMREVRAAFALERDITFNQVTAKLPYMLACLNEAFRLYPPVPGGLQ

RWTEVPTWISGYRVPAN (0)

TKVSVHQLSAYSSLKNFHQADRFIPERWLPEAIEDPASPFFSDNRAVFQPFSIGPRNCLG

RNLAYTEMRVILARVLWTFDLTLCEESRDWKDQKIFVIWERGPLMCKLAMRDDRGDCQGGR*

 

>CYP65T2P  Aspergillus fumigatus Af293

          XP_748490.1 also AAHF01000010.1

          58% to 65T1 Nterm aa 52-168 plus C-term part, 84% to 65T2 = ortholog

          name revised from 65T3P

SLRGRLDLDLRSWHEKYDGVVRPGPDEVTFITAQAWKDIYGHGHLQLPKVQISTINGK

NIFATNDVDHARFRKALSHAFSAKGLQAQECLVTRYIDKRIERLKGFTESGTAADMGKW 425909

*NLATFDLIGDVAFGEPFGGLD

 

AYSSPRNFHQADRSIPERWLPQVIENPASPFFLDNRAVFQPFSIRPRNCLGRNLAYTEMR

MILARGLWTFXX

LCEESRDWTDR

 

>CYP65T4  Aspergillus oryzae

         GenEMBL BAE59716.1

         68% to 65T1, 45% TO CYP65AF1

MPFNILLWPVIAFFLYSLLTAIWYIYFHPLRRIPGPKSWIIFPIMRHASAIRGRFDIDMRQHHAVYGPVV

RFGRDEVSFITAEAWKDIYGHGHQQLPKVLSSASNMLDIISSNDTDHSRYRKALSHAFSAKGLQAQEPLL

NSYVDKLIERLKGIAESNLPADMVKWYNLTTFDIIGDLAFGEPFGGLDNSEYHHWVSTIFESIKAIPFLK

LKDAYPLAFKAILGLIPKGIMEARKRQLEHSRITVQKRLQTSSSYNRGDFMDSMLRNRGEKDSLNDSELE

ANSNILIIAGSETTATLLSGATYWILRNPEALAKLTDEVRSVMKSEPEITAQKASAELPYMLACFDEAFR

LYPPVPTGLQRRTLVPTRISGCDIPAGTKVSVHQSAAYWSSTNFHAPDRFIPERWLPEAKSDPSSPFYSD

NRGVVQPFSTGPRNCIGRNLAYAEMRVILARVLWNFDLELCEESTQWSDQKAYTLWEKPPLMCRLKLRES

FRG

 

>CYP65T4  Aspergillus flavus

99% to CYP65T4  Aspergillus oryzae

AFL2G_07016 revised

MPFNILLWLVIAFFLYSLLAAIWYIYFHPLRRIPGPKSWIIFPI

MRHASAIRGRFDIDMRQHHAVYGPVVRFGRDEVSFITAEAWKDIYGHGHQQLPKVLSSASNTLDIISSNDTDHSRYRKAL

SHAFSAKGLQAQEPLLNSYVDKLIERLKGIAESNLPADMVKWYNLTTFDIIGDLAFGEPFGGLDNSEYHHWVSTIFASIK

AIPFLKLKDAYPLAFKAILGLIPKGIMEARKRQLEHSRITVQKRLQTSSSYNRGDFMDSMLRNRGEKDSLNDSELEANSN

ILIIAGSETTATLLSGATYWILRNPEALAKLTDEVRSVMKSEPEITAQKASAELPYMLACFDEAFRLYPPVPTGLQRRTL

VPTRISGCDIPAGTKVSVHQSAAYWSSTNFHAPDRFIPERWLPEAKSDPSSPFYSDNRGVVQPFSTGPRNCIGRNLAYAE

MRVILARVLWNFDLELCEESTQWSDQKAYTLWEKPPLMCRLKLRESFRG*

 

>CYP65T? fragment   Aspergillus ochraceus

         DQ054597 C-term piece after heme signature

         Involved in ochratoxin synthesis

         See DQ054596 for a second P450 in this pathway

         This fragment is 60% identical to CYP65T5 C-term

FLRAQGTASERNLAYAEMRLIAVKLLWNFDLAFEEECEGWDNQK

SYNIWEKDPLKVKLTP

 

>CYP65T5   Aspergillus terreus NIH2624

          NW_001471196

          68% to 65T4, 62% to CYP65T1

          ATEG_08832.1

MALQEFLPVVPAISVLYIFLLAIWNLYFH

PLRHIPGPRSWIAFPIMRHISASRGRLDSDMRRFHAQYGGAVRLAPAEVSFITPDAWKTI

YGHGHTQLPKVQTSESKGLDIISSEGPNHTRHRKALAHAFSARGLQAQEPLVRGYVDKLI

ERLKEFAESQLQVDMVKWYNLTTFDLIGDLAFGESFGGLDNSRVHSWVSTIFRSVKVLPF

VRITDTYPILIPLLMALLPKSLRTARRDQTNYSKETVHKRLANTAAHGRGDFMDSMLRHR

GEKDGLSDRELEENASILIIAGSETTATLLSGVTYWLLRSPEALAKVTDEVRSTFQTEGE

ITLQDVGARLPYMLACLDEAFRMYPPVPCALERRVLTPIVIAGYNIPPG (0)

TVVSVHQSAAYCSPANFHRPQDYIPERWLPDAKSNPSSPYFSDQRDVLQPFSVGPRNCIG

KNLAYAEMRLILARVLWNFDLELCEESLHWKDQKSYLLWDKPPLMCKLKQRI*

 

>CYP65T6 Aspergillus niger

e_gw1.4.330.1|Aspni1

65% to CYP65T4

MPTILAIVVISYIVYTVCAAIWNIYLHPLHRIPGPKLWLAFPILRYISFMRGVLDRDMRTYHNRYGGAVRFSPNEVSFIT

TQAWKDIYGHGHRQLPKVPGSMNNPMDIISANDSDHTRFRKAISHAFSAKGLQAQEPFIMEYVDKLINKLHSMAKSQVAV

DMAKWYNLTTFDLIGDLAFGESFGGLDSTEYHYWVSTIFEFIKAIAFARFKDNYPMMFQVLERFLPKHLLEAKRRQDEYS

WNTVQKRLHHQRDRGQADFMQSMLRHRGEKDGLTDEELAANASILVIAGSETTATLLSGLTYWLLQHPKEMEKVKFEVRS

VMKTEEDIDVNNATAKLPYMLACIDEAFRMYPPVSGGLQRYVPDTPTEISGYLLPPKTNVSVHQSAAYRSPLNFYRPEEF

IPERWLPQSKNDPSSPFYHDNRDVLQPFSVGPRNCIGRNLAYAEMRVILARVLWNFDIELCEESRDWHDQKSYNLWAKPP

LMCRLKARVH*

 

>CYP65T7 Aspergillus niger

fgenesh1_pg.C_scaffold_3000290|Aspni1

68% to CYP65T4

MLDPLILAIAPGIYFLYSILSIIHALYLHPLRHIPGPKSWIILPLFRHISAVRGRLDADIHRFHEKYGPVVRVTHNEVSF

NTAEAWNRIHGYGHSELPKVSSSGANPRDIVSANAADHSRFRKALSPAFSAKGLQAQEPLINSYVDKLISRLKDIAESQL

PADMVKWYNLTTFDIIGDLAFGEPFGGLESSQYHYWVATIFQFLKLLPFLKLRDAYPTIFKLIALAMPKTLLNARERQRA

HTRITVQKRLDNASKYDRGDFMDSMLRNSGTKDGLTHEELVANANILIIAGSETTATILCGVTYWLLKSPDALAKVTTEV

RSIMKSETDISVSSVAAKLPYMQACIDEAFRMYPPVPTGLTRMTIEPTLISGYEIPPGTKVFVHPIAAYHSPRNFHAPER

NLPERWLPEAKTDPASPFFSDNREVLQPFSIGPRNCIGRNLAFAEMRVIIARLLWNFDIEICEESKDWSDQKTFTLWEKP

PLMCKLSLRN*

 

>CYP65T8 Aspergillus clavatus

69% to CYP65T2 Neosartorya fischeri

ACLA_062600

MLHLRERAAIANVTGVVSVLYSARSVIKRAGSFPDVGHDPKYRAVLLDLIVKMFLALGTALALYLGYILASAIRNIYFHP

LRDIPGPKSWIAFPLLQHLSAIRGRLDLDMHHWHIKYGAAVRFDPTSVSFITADAWRDIYGHGHKQLPKVLNSGSNTQDI

ISANDADHTRHRKALAHAFSAKGLQAQEPIVTGYVDQLIARLRDVAASGLPADMVKWYTLTTFDLIGDLAFGEPFGGLES

SEYHRWVAAVFGFIRVIPFLKGMDEYSVLFRVILSFLPRSFLQMRTDQVEHTKRTVQKRLRTRRHDRSDFMDSMLRHRGD

KDGLSEEELVANANILIIAGSETTATLLSGVTYWLLQTPAAMERVTREVRTTFASEAEITFNNVTAQLPYMLACISEAFR

LYPPVPGGLQRWTETPTRISGYEIPGRTKVSVHQAGAYWSSRNFHQPDSFIPDRWLPEAKDDPASSFFSDQRDVLQPFSV

GPRNCIGKNLAYTEMRVILARVLYNFDLQLCEESRDWKDQKTFVLWEKKPLMCRLTARRE*

 

>CYP65U1 Aspergillus nidulans AN6466.1 37% to 65B1 53 clan

revised 7/18/07

MGVYLFSSSLWATLLLAILIYRLVRITYNLYFHPLSGIPGPSAWAASRLP

FVYALIRGTIIQDFQKLHEQYGPIIRIAPNEVTFAHPEAYTDIFQPRPEK

SQGQFLKDPLWWARQPGHPDSLLSVINPERHAQMRRVLSPGFTARALRKQ

EPFVQKYVNLLVAQLQDLVSKSPSAHINMTPWFNYTTFDIFGDLGFGESF

DCLQHSRYHPWIALLFDSVKAAGFVIATRYYPLIEAVLLKCVPPSMRKIQ

RDHYQQIVDKVQRRLGWELQRPDFMSYVIDERGGLRLDAGELYATFMILT

TAGSETTATALTGTLNYLVNHSPESLQRLENEIRGAFSGLDAITLDAVRN

LPFLNAVIQEGLRLCPPIPWVLPRLVPQGGSMICGTWLPGGTPVSIQAYT

LNRDPTLFNRATSFLPERWLASSTSDSSSCFFNDQRQVVQPFSTGPRACL

GQHLAWAEMRLILAKLVWVFDFGAIDGQGVNWEDLRTYLLVERKPIN

VRISSRTV*

 

>CYP65U2 Aspergillus niger

e_gw1.3.403.1|Aspni1

61% to CYP65U1

MAINALSIAISLLVIVCIAFPTWQLFLLQNQLLKLFAQYPISRIVYNVFFSPLSHLPGPISWSATRLPFTRALLRGTIVH

DFERLHRKYGPVVRTAPDEVSFASGDAWTDIYASRPDDRQFLKDPLWWRRQPGQPDTLLSAIHPAKHSRMRKLLAPAFTP

RALRVQEAVLQRYASLLVDRIKDQVSVAGTDGAVIDMGPWFNFTTFDIFGDLGFGESFNCLQHSQYHPWIVLLFGSVKAA

SFIAAARYYPPLEALLMKCIPRSLHEKSQRHYRQIINKIDRRLSWELQRPDIMSHLMDENGQVALPRGELNSTFMILTTT

GSETTATVLTGILTYLVNQPEVLGRLIGEIRGRFGSSQDISLSEAADLPYLTAVIQEGLRLCPPVPWMLPRQVPPGGSTV

CGTWLPGGTAVSLQAYTLNRDPSRFHAASSFLPERWLPDASSNPNSNFYQDDRHAVQPFSMGPRSCLGQHLAWAEMRLIL

TKLLVNFDFEAVEGKQLRWEELRTFLLVEKRPLEVRVRLA

 

>CYP65V1 Nectria haematococca

gw1.24.106.1 model short on N-term

Necha1/scaffold_24:57028-58643

MLFALAL (0)

AILYLLGSIFYNLFLHPLRKIPGPFWMRATRATYCYKLVFGTLPFD

MLDLHNHYGDVVRIAPDELAFSGREAW

KEIMGHRIKDNKRPTFEKYDQFYRPNGGPTDIANSSGTEHQVLRRLLSHGFSDRSLRDQEPIVKKYVDLL

IQRLHENSDRPLDIMSWYNYTTFDVIGDLAFGEPFGCLDNSDYHPWVRIIFEVARFGTVNQTAGYFPLLR

SLLTRILSSKAVRSRGESHRALTREKMLRRVELGKNGGRPDLIDNLLKKSEEL (0)

GITEAQLLKNCGALIIGGSETTATLLSGVTYLLLKTPEALHKLTGEVRSAFETEDEINFTSVN

KLTYMLACLDEAFRVYPPIPGGLPR QIPKGGSEVSGRFIPGD (0)

TVVAIHQWAMYHSEKHFRDPFNYHPERFLGDSNFESDHRDVLQPFHVGPRNCLGRN (2)

LAYVEMRVILARIIWNFDLSLAEESQDWLQRQK VYIFWDKGPLIVNLTP (0)

RV*

 

>CYP65V2  Aspergillus oryzae

         GenEMBL BAE61720.1, AP007163.1

         53% to 65V1, 43% to 65B1

         revised 3/20/2009 with a frameshift

MMLNSINIPQNAISWAVACAAA (0)

198235 ATFCLASSVVYNIFFHPLARYPGPLSHAASRVPYFIRVFKGTLPFD

MLDFHTRYGDVVRIAPDELAFSHPDAWKDIMGHSKGGLYLEKASWFYRP

LEYDPPHIFNEGRQAHSLLRRQLAHSFSDKGMRDQEPMIRGYVDLLLQRLREMGGREDTVDISAWYNYTT

FDIIGDLSFGESFGCLKGSNYDEWIESIFRAFPFITALQALSFTPLVKEVLLALVPPSMRDRRERLRDFT

RQKMLRRMAIAEERPDLIEGLLKKKEEL (0)

GLTIDKLTA &

AETLVVAGSETTATLLSGVTYLLLKNPEAYQ 199191

RLREEVRSTFSSEDDINLSSAAKLPYMLACVEEALRMYPPGAIGPPRVTPKGGAEILGARIPGR (0)

TYVSIHHWALYRREEYFTDPHTFHPERFLGDPRFSNDRHNVLQPFHIG

PRSCLGRNLAYSEMRLILALLFFNFDMELSDDSQDWIQQKNVIMWQRGPLKVHLTYIHRNSA

 

>CYP65V2  Aspergillus flavus

99% to CYP65V2  Aspergillus oryzae

AFL2G_09433

MLNSINIPQNAISWAVACAAAATFCLASSVVYNIFFHPLARYPGPLSHAASRVPYFIRVFKGTLPFDMLDFHTRYGDVVR

IAPDELAFSHPDAWKDIMGHSKGGLYLEKASWFYRPLEYDPPHIFNEGRQAHSLLRRQLAHSFSDKGMRDQEPMIRG

YVDLLLQRLREMGGR

EDTVDISAWYNYTTFDIIGDLSFGESFGCLKGSNYDEWIESIFRAFPFITALQALSFTPLVKEVLLALVPPSMRDRRERLRDF

TRQKMLRRMAIAEERPDLIEGLLKKKEEL (0)

GLTIDKLTANAETLVVAGSETTATLLSGVTYLLLKNPEAYQRLREEVRSTF

SSEDDINLSSAAKLPYMLACVEEALRMYPPGAIGPPRVTPKGGAEILGARIPGR (0)

TYVSIHHWALYRREEYFTDPHTFHPERFLGDPRFSNDRHNVLQ

PFHIGPRSCLGRNLAYSEMRLILALLFFNFDMELSDDSQDWIQQKIVIMWQRGPLKVHLTYIHRNSA

 

>CYP65V3P  Aspergillus oryzae

          GenEMBL BAE55600.1, AP007151.1

          Gene model has wrong C-term, revised

          about 60% to CYP65V2, 48% to 65B2, 54% to 65V1

          one stop codon and one frameshift in last exon

MSTAALVNAKSVLIATISA (0)

PIIYMLGMAIYNVYFHPLNRFPGPVSHAISRIPYFYRAVRGTLPFD

MLKLHERYGDIVRIAPDELAFSHPDAWKDIFGHKNGEPEMAKAAWFYRPLDDPLHIVNEDTDEHRRLRRQMAHGF

SEKSMRAQEPIIRKYVDLSLEKLHQSCKNGSLVILDWYNFTTFDIIGDLAFGEPFGCLEGCNYDQWIKGI

FKGAYLGSFMQALSFVPRLKSTLLLLVPKSMQEAHQGHKALTKAKMLRRAAITEKRPDLIDGLLRNKDEL

1455183 KLGLDKLIANAEILIIGGSETTASLLSGVTYLLLQNPNAYETLKNEVRSKFNHQEEIN 1455356

LISVNKLSYILA

CLDEALRMYPPIANGLPRVCPKEGSWVLGEYIPGKTVLSIHQWALYRREKHFKDPNTYHPERFLSSPEFL

DDRRDAFQPFHTGPRNCLGRN (2)

LAYS*MRLILALVIFNFDMKIAAESK &

DWIRQRNYLMWDKGPLKVYLRPRTSI*1455965

 

>CYP65V3P Aspergillus flavus

98% to CYP65V3P

AFL2G_00556 gene model revised, one frameshift in last exon

MSTAALVNAKSVLIATISA (0)

PIIYMLGMAIYNVYFHPLNRFPGPVSHAISRIPYFYRAVRGTLPFDMLKLHERYGDIVRIAPDELAFSHPDAWKD

IFGHKNGEPEMAKAAWFYRPLNEPLHIVNEDTDEHRRLRRQMAHGFSEKSMRAQEPIIRKYVDLSLEKLYQSCKNGSLVI

LDWYKFTTFDIIGDLAFGEPFGCLEGCNYDQWIKGIFKGAYLGSFMQALSFVPRLKSTLLLLVPKSMQEAHQRHKELTKA

KMLRRAAITEKRPDLIDGLLRNKDEL (0)

KLGLDKLIANAEILIIGGSETTASLLSGVTYLLLQNPNAYETLKNEVRSKFNHQ

EEINLISVNKLSYILACLDEALRMYPPIANGLPRVCPKEGSWVLGEYIPGK (0)

TVLSIHQWALYRREKHFKDPNTYHPERFLSSPEFLDDRRDAFQPFHTGPRNCLGRN (2)

LAYSEMHLILALVIFNFDMKIAAESK &

DWIRQRNYLMWDKGPLKVYLRPRTSI*

 

>CYP65V4 Aspergillus niger

e_gw1.11.216.1|Aspni1

70% to CYP65V3P

MTLPDIFYHPPADDHDSTHHAHNNRSNATERERGVGGTCFRGNGPSLVYNICDAQSNLAAQVTIYYATQIIYNVFFHPLS

RFPGPISHAISRLPYCYYALIGTLPFHMLDLHNQYGDIVRIAPDELAFSHPNAWKDIMGHKNGEEELGKADWFYRPLPEA

RHIVNEERDQHKGLRRAMAHGFSEKAMREQEPLIRKYTDLLLERLREQSKSGQSVAISDWYNFTTFDIIGDLAFGEAFGC

LENAKYDYWIKTIFESGRLGIILQSISFYPILKSLLLLLVPKSLQEAQQKHKALTTTKMLRRLAILEDRPDLINSLLKKK

EDLALTMDHLIANAEILIIGGSETTASLLSGVTYLLLQNPDAYQNLRDEVRSTFRKQDEINLISVNKLTYMLACLDEGLR

MYPPIANGLPRMCPRGGCTVLGHYIPENTYVSMHQWALYHREKHFTDPESYHPERFMGSPQFANDRRDVLQPFHVGPRNC

LGRNLAYSEMRLILALIIFNFDMVISEDCDDWIQQKNFLMWQKRPLKVYLKPVPRDNP*

 

>CYP65V5 Histoplasma capsulatum

ABBT01000064.1 Ajellomyces capsulatus G217B

55% to CYP65V4

HCB02008.1

254348  MAVIVKIQALTQSPVILAATSLSIVVLYLISRFIYSVFFHPLRSFPGPTSHAMSQIPYIYKSLRGTLEFDILDLHKTYGD

IVRIAPNELAISGPGAWKDILGNRRSGEEQLEKFQQYYRPTGGKKATSIINADRDDHSRLRRQLAHGFSDKSMREQEPLI

TQHIDLLVKRFHERGRGGSKPFDLTQWYNYATFDVIGDLTFGESFGCLENAYFHPFVKLMLASGKIAVFLQCLSFFPLAKETFLNLIPR

SALKSQANVAKE

KILRRMQMGIERPDLIEGLLKKKDEWNMSLDALSGNARLILVGGSETTATLLCGVTYLLLSNPQTLERLTAEVRSAFKSE

DEINMSSVNDLNYMLACLDEALRVYPPVPLGLPRVVPKGGCEITGQFIPEDTVVAVHHWATYHNEKYFTDPFAYHPERFL

GDPKYASDKRDLLQPFHIGPRSCLGRNLAYAEMRLILARLIYNFDMRLAKESADWLAKQKVFLFYTKPPLNVYMTPVNSRE 256403

 

>CYP65V6 Aspergillus terreus

57% to CYP65V5

ATEG_02650.1

MAVLTGDPSSLLAVAVGL (0)

VIVHITAKALYNIFFHPLRSFPGPPSHAAFRFPYCYK

LLRGTFPFDMLSLHRQYGDVVRVAPNELAFSHPDAWRDIMGHRIDGSDEMGKWDQFYRAVPQTPTSIVSADRTEHGALRR

QLSHGFSERAMRAQDPLITGYVDLLIQRLHENCRGGAQTVDMTAWYNFTTFDIIGDLAFGQPFGCLEKSEYHPFVPLLFK

SSRMGSVVFSLSFYPMLKKLVFAMIPKSLIQLFEDHRQVSIEKLRERMKLEVERHDLIEGLLRKKDDLVCLG &

MDHLEANANVLLIAGSETTATLLSGVTYLLLMNPEALSRLTEEVRDAFNSEAEIDMQS

VSRLPYMLACLDEALRMYPPVPNGLPRVVPKNGAQIAGKFV ()

QTPVAIHHWASYHNEKYFADPFNFHPERFL

GDERFANDRFDILQPFHVGPRNCLGRN (2)

LAYTEMRLILARVIYNFDMKLADDSTDWLKRQKVWFFWNRPALNVYLTPVKR*

 

>CYP65W1 Nectria haematococca

e_gw1.5.258.1

Necha1/scaffold_5:405215-406714

MLAIGLWLLAALPLL (0)

ALLKIIYNVLLHPLRHHPGPKLWASTRIPWCWNQYQGKLNHRLVELHHQYGPAVRVAPDEVSYTSDTAWKTIY

GHRSVEMTKDPIFSLVTPTGVP (1)

NIVTADRVNHSRHRRLLSHAFSEKALREQEDILVQYCTKLLDQLQARCSDGPLDLAKWFPLT (1)

TFDLIGSLAFGQDFSCIDTGEPHPFVACIKGVSRELVMSQMAQYYGFGSLRYFLFL

RGSANSRFANAMRAKEMVEARIARGPTDDRKDFWHHVLAAEDQGKGLTPDEMVVDAFSIAIAGSDGTATA

LAGTTYLLLTHRDVYQQLQGFIRQEFEAEEDITTLALSNERVPLLGAVIQESIRLFPPVAATLPRVVPPS

GETIDGLFVPEGTIVGVNQLAAYHSEKNFNQASEFLPERWLSEQGDCRAVFQPFSFGPRNCLGKNLAKAE

LRLILARMLWRFDMELLPESQGWMDGQKIYGFWIKPPLMVKLSPRAN*

 

>CYP65W2 Aspergillus niger

fgenesh1_pg.C_scaffold_14000165|Aspni1

53% to CYP65W1

MALIQVVGSDSMFWLGAVALTSLLAWVILHVCYNAFLHPLRQYPGPRLWAATRLPWCWYQAHGRLNQKLLELHLRYGRIV

RVAPNELSYNSEEAWRAIYGLRSDEMGKDPVFSLRTPTGVQNILTADRQTHTRQRRLLSHAFSEKALREQEGIIQGYVDQ

LMKQLAARANDGPQNMVDWFTFTAYDLIRDLSFGERFHCLDTAEYDPFVRSIQAISKELTFIQMLAYYKLLSLRQLFMSK

AFSGARAQNMQRVIETVNRRVDRNTDRKDFLHYILAAMETEKGMSRAEMNVNAFSFSIAGSESSATALSAFTYYTLSRVS

VYDRLIAEIRGTFDAYEQIDISSTAQLSYLNAVLQETLRIYPPVAVTLPRVVPVNGTVIDGDFVPAGVTVGVNHFACYHD

PRNFHRPQEFLPERWLPECQAQGPFSKDCQKSCQPFSFGPRNCLGKNLAWAEMRLIAAKLLFLFDMEIDETSKGWTDGQK

VLGFWVKPPLFVRLMQRGC*

 

>CYP65X1 Aspergillus oryzae RIB40

AP007157.1c genomic DNA, SC023

join(331674..331760,331832..332607,332687..333038,

333095..333264,333318..333474)

third P450 gene in a set of 8 on this accession

42% to CYP65S2, 40% to CYP65S3, 42% to 65A1

MALAAEYFSHALSQPYGSSFWVAVAASLVFICYITCDTVYNLCL

SPLSSFPGPRLWAVSNIPRQLSILGGRSHLKMLALHHRYGPVVRVGPSELSFNSPQGF

RDIYGFRRGQPQFQKDPKMYGSPLTGISNSIGGHVDSDTHSRHRRLLSHSFSERSLRE

QEGIVVYYVDLFIQRLRERTSVNKIHRAEEDLKSWFNFTTFDIIGDLMFAETFDCLKD

SQLHPWIALMFNNVKGIAFLGVLNEYSLFRKMQGALLPKALKQKMLENHKLCAQKADR

RLQKGASRPDFVSAILKHGLSDDKEEFIENQPLMSRAEIHANSIF (2)

ITIAGSETTASLLSGCLFYLC

KHKYIMDQLNKEIRTTFSKDEEITSSKCFNLSYLNAVLKESLRLYPPVAASLLRLVPK

GGCTIDGHFVPED

VTVSTHHYASYRDAANFTFPEQFIPERWLGTDNRFDSDRKDVVQP

FSLGPRDCLGKNLAHMEMRLILSKLLFNFDIHLTPESENWGQQKMFIVWDKPALMVRL

TDRFA

 

>CYP65X1 Aspergillus flavus

99% to CYP65X1 Aspergillus oryzae, 48% to CYP65AU1

AFL2G_04023

MALAAEYFSHALSQPYGSSFWVAVAASLVFICYITCDTVYNLCLSPLSSFPGPRLWAVSNIPRQLSILGGRSHLKMLALH

HRYGPVVRVGPSELSFNSPQGFRDIYGFRRGQPQFQKDPKMYGSPLTGISNSIGGHVDSDTHSRHRRLLSHSFSERSLRE

QEGIVVYYVDLFIQRLRERTSVNKIHRAEEDLKSWFNFTTFDIIGDLMFAETFDCLKDSQLHPWIALMFNNVKGIAFLGV

LNEYSLFRKMQGALLPKALKQKMLENHKLCAQKADRRLQKGASRPDFVS

AILKHGLSDDKEEFIENQPLMSRAEIHANSIF

ITIAGSETTASLLSGCLFYLCKHKYIMDQLNKEIRTTFSKDEEITSSKCFNLSYLNAVLKESLRLYPPVAASLLRLVPK

GGCTIDGHFVPED

VTVSTHHYASYRDAANFTFPEQFIPERWLGTDNRFDSDRKDVVQPFSLGPRDCLGKN

LAHMEMRLILSKLLFNFDIHLTPESENWVQQKMFIVWDKPALMVRLTDRFA*

 

>CYP65Y1  Aspergillus fumigatus Af293

         GenEMBL XP_746900.1

         49% to CYP65V1, 88% to CYP65Y1 Neosartorya fischeri

revised 3/12/2009 added N-term, one stop codon

MGVGSDKG*VVCYVITQAVYNVFLHPLSRFPGPLLHRASRLAYVYRLIGGTLTLDALE

MHQRYGDIVRVAPDELFFAHPDAWSDIHKKTGREMDKAPWFYRPLEQSPRCIVDESHEQHSRLRRQMAPG

FSEKSMRDQEPIIRGYVDLLLQRLLEKSNDGQPVVISDWFNYTTFDIIGDLVFGEPFECLGSSKYDGWIK

SIFESLRPLTIVQALSFFPWLKRIALDTVPKNLQGDIKQQQQQTEAKMRRRMAVTASRGDLMEGLLKKKD

ELHLSTEELIGNAQVLILAGSETTASLLSGVIYLLLQNAVAYRQLVDEVRSTFNSEKDINFVSVSQLSYM

LACLNEALRIYPPVANGLPRVVPKGGAQILGQYIPEQTYVAIPQWALYHREQYFAEPNDFHPERFLGDPK

FANDRRDALQPFSVGPRNCLGRNLAYAEMRLILALVIFHFDMKIDPDCRDWIQQRNFTLWQKPPLKVYLK

PVARKSEPNQV

 

>CYP65Y1 Neosartorya fischeri

88% to CYP65Y1 Aspergillus fumigatus = ortholog

NFIA_112820 revised

MGVSSDKGQVVCYVITHAVYNVFFHPLSRFPGPLLHRASRLPYFYGLIKGTLTIDALE

MHQRYGDIVRVAPDELFFAHPDAWSDIHKKTGKAMDKARWFYRPLEVTPRSIVDESHERHSRLRRQMAAGFSEKSMRDQE

PIIRGYVDLLLQRLREKSNDGQPVVISDWFNYTTFDIIGDLAFGEPFECLKSSKYHGWIRSVFESSRPLAVVQALSFFPW

LKRIVLDMVPKDLQETFKRQQRHTEAKMRRRMAVTGNRVDLMEGLLKKKDEFNLSIDELITNAQVLILAGSETTASLLSG

VIYLLLQNSVAYQQLVDEVRSTFSSEKEINFVSASQLSYMLACLNEALRMYPPVANGLPRVVPKGGAQILGQYIPEQTYV

AIPQWALYHRKEYFAEPMDFHPERFLGDSRFANDRRDALQPFSVGPRNCLGRNLAYAEMRLILALIIFNFDMKIDPDCRD

WIQQKNFTLWQKPPLKVYLKPVARKSEP*

 

>CYP65Y2P fragment Histoplasma capsulatum G217B

C-term pseudogene of CYP65Y sequence 67% to 65Y1

ABBT01000195.1 Ajellomyces capsulatus G217B

HCB05094.1

ERFLGDPKFANDKRDMIQPFHVGPRNCLGRN (2)

LAYAEMRLILARLIYNFDMKLAPESANWMRKQRVFLFYTKPALNVYMTPVKR* 131686

 

>CYP65Z1  Aspergillus fumigatus Af293

         GenEMBL XP_751828.1

         41% to 65R1 Fg, 44% to 65S3

MAPWNHDIFDRLMLSAILSWSILLQSQIANYRRRTLTRRQGCVYLIGHSIYAIYFHPLSRYPGPKIAAIS

PIALLVWEIRGKVHSKVKHLHDRYGDVVRIGPNALVYRAPRAWKEIYGYRTKKGQRTFQKDPSLYVPTPN

GVHAIITAAESDHIRMRRLLAHAFSDRALREQESLLHFYADLLVQKLHENLNHSHSEVVDIARWYNFTTF

DLIGDLAFGEPFQCLKDSKYHWWVSIMLDAVKLSVYLKVLWFFPILSPLTKLLVPRYLHQRREASFQLTV

EKVSRRLHRQTNRPDFISYILRHKDDENRMSRQEIDANAATFVLAGSETTAALLSGCTFYLLKNPHIYRR

LVTEIRSRFQHPSEIRLSSIATLSYLNAVLEESLRIYPPIPAMLPRLVPEEGAMINGEYVPAGTSVSMSL

WSTFHSSGNFHNPDSFVPERWLASPEEEASRCFTMDKKEAFQPFSYGPRNCLGQHLANAEMRLILAKVFW

HSDMELCPESENWADQESYNLWSRPPLMVMISRANGRNYEDNMV

 

>CYP65Z1 Neosartorya fischeri NRRL 181 XM_001267015

94% to CYP65Z1

name revised from CYP65Z3

NFIA_106070

MLTRQQGSIYLIGHSIYAIYFHPLSRYPGPKIAAISPIALLVWE              IRGRVHSKVKDLHDRYGDVVRIGPNALVYRTPQAWKEIYGHRTKKGQRTFRKDPSLYV

PTPNGVNAIITAAESDHIRMRRLLAHAFSDRALREQEPLLHFYADLLVQKLRENLDHS

HSEVVDIARWYNYTTFDLIGDLAFGEPFHCLKDSKYHWWVSIMLDAVKLSVYLKVLWF

FPILSPLTKLLVPSYLHQRREASFQLTVEKVSRRLHRQTNRPDFISYILRHKDDENRM

SRQEIDANAATLVLAGSETTAALLSGCTFHLLKNPHIYRRLVTEIRSRFQHPSEIRLS

SIATLSYLNAVLEESLRIYPPIPAMLPRLVPEEGAMINGEYVPAGTSVSMSLWSTFHS

SANFHNPDSFVPERWLASPEEETNRCFTMDKKEAFQPFSYGPRNCLGQHLANAEMRLI

LAKVFWHLDMELCPESENWTDQESYNLWSRPPLKVMISRADGRYEEDSMV

 

>CYP65Z2P Aspergillus fumigatus

87% to CYP65Z2 Neosartorya fischeri

supercontig 2 3641455-3643345 region

MSPVSIVLSL (0)

LVAVLLGQVIYNI &

VIYLQPLRKSPGPKLVACSNICYIWWTFSGEIHAKLKELHDQYGDVVRISP &

STLVYRSPGAWKDIYGHRKHGVNSFIKDPEFYNPSPNGGHILTAGDADHARQR

RRLSHAISE &

KTLREQESLVLHYVDLLVEKLRAECAAARSTVDMMKWYNYTTFDIFGDMAFGELLNCLRD

NRYQPWVVMVYQSVKMSIDLRLIHLYPLLESLATRLL &

PKKAAEMRNEHWRMSKDKVGHRLDLQATRPDFLRHNDERGMTRQEIEENAGVL

ILAGSETTATLLSGCPFYVLKHPEKYNKLVQEIRGAFQKQEDITFLSVARLPYSHAVLEE

SLRLYPPVPGYLPRKVPMGG &

ASIDGQYVPEG (0)

ISVSVVPFLA

SRAKSNFVEPESFIPERWLENRYPRFETDHREASQPFSFGLRNLLGK (2)

NLAYAEMRLIVAKLLWNFDMTLYEGCRDWGDQTSYIIW*KPSLMVELKAVR*

 

>CYP65Z2 Neosartorya fischeri NRRL 181

XP_001260852.1, NFIA_089120

56% to CYP65Z1 Aspergillus fumigatus Af293

76% to CYP65Z5 Aspergillus clavatus

MSPISIVLSILVAVLLGQAIYNIYLHPLRKFPGPKLAACSNICY

IWWTISGEIHAKLKELHDQYGDVVRISPSTLVYRSASAWKDIYGHRKHGASSFIKDPE

YYIPSPNGGHILTAGDADHARQRRLLSHAFSEKAMREQESLVQHYVDLLVEKLRAECA

ASRSTVDMMKWYNYTTFDIIGDLAFGEPFDCLRDNRYHPWVKMVFQSVKMSVYLRLIH

LYPLLESLVKRLLPKKAAKMRNEHWRMSKDKVSRRLDLQTSRPDFMSCILRHNDERGM

TRQEIEANAGVLILAGSETTATLLSGCTFYLLKHPEKYNKLVQEIRGAFQKQDDITFL

SVARLPYLHAVLEESLRLYPPVPGYLPRKVPMGGASIDGQYVPGGVSVSVVPFSASRA

KSNFAEPESFIPERWLENRDRRFETDNREASQPFSFGPRNCLGKNLAYAEMRLIVAKL

LWNFDMTLHEDYRDWDDQTSYIIWQKPSLMVELKAVR

 

>CYP65Z4 Aspergillus clavatus

81% to CYP65Z3

ACLA_049200

MAFWLHYILESISLWAIFKLGLLVALSYLAPHSIYAIYFHPLSQYPGPKLAAISPIPLLVWEIRGIVHSKVKDLHDQYGD

VVRIGPNALVYRTPQAWKEIYGHRTRKGQRTFAKDPALYVPTPNGVNAIITAPESDHIRMRRLVSHAFSDKALRDQEPLL

HTYADLLVEKLQACIESSRSGVIDITRWYNYTTFDLIGDLAFGEPFDCLKDSKYHWWVSIMLDAVKLSVYLKILWFFPFL

APLTKLLVPRSLHQRREASFQLVVDKVDRRLQRQTDRPDFISYILRNKDDESRMSREEIDANAATFVLAGSETTAALLSG

CTFNLLKNRRVYERLVSEVRDRFQDPAEISLSSIATLPYLNAVLEESLRIYPPIPAMLPRLVPEEGAMINGAYVPAGTSV

SISLWSIFHSSTNFAEPDAFVPERWLKNQADENEADNPFAGDKKEALQPFSYGPRNCLGQHLANAEMRLILTKVLWHFDM

DLCPESEDWAEQESYNLWCRPALNVKLSAADGGNRETMM*

 

>CYP65Z5 Aspergillus clavatus

76% to CYP65Z2

ACLA_073680

MTSMSMSHLLEPLSMVCGIILLVSPLSLAFAVKTLAVPVAQSLYNIYFHPLRRFPGPKRASCSNVCYLWWTVSGTIHWKL

KELHDQYGEVVRISPSILVYRSPNAWRDIYGHRKHDTTSFVKDPDYYIPSPNGEHILTANDADHARERRLLSHAFSERAL

REQESIVQQYVNSLILKLQEYAASGSAVDMAKWYNFTTFDIIGDLAFGEPFDCLRDNRYHPWVWMIFQSAKASVFMRLLH

LYPRLESLVKKMLPKKFVRMRNEHWQMSKEKVGRRLSLQANRPDFMSYILKYYDERGMSRQEIEANAGVLILAGSETTAT

ALSGCTFYVLKNPEVYNRLVREIRTSFQRQEDITFLSVARLPYLHAALEESLRLYPPVPGILPRKVPAGGASVDGQYVPG

GVTVSTASFSAARAKSNFADPDSFIPERWLKNRDPKFESDNREASQAFSLGPRNCLGKNLAYAEMRVIIAKLLWNFDLSL

HSDCYDWDNQASFIIWQKPALMVDLKPIRG*

 

>CYP65AA1  Aspergillus fumigatus Af293

          GenEMBL XP_749877.1

          43% to 65S2, 40% to 65Z1

MSFLMESSAVGQPSPLSFSNTLNALPGAL ()

VLYAALHSLYNIYLHSLSRFPGPKLWQTSYIFRHIASIRGT

LDVSIKAFHTEYGPVVRYSPDELSFISAEAWRDIYGFRENALPKDPSFYGLIQLSRDKSPSIFTVDQDHH

PRVRKALSYAFSEKALHDQEPFVKHYVDLLIQRLRGIADAEDNRVDLVKWYNFTTFDIIGDLAIGRSFDC

LQDSAYHSWVDAFWKSIKMISPYARAMATYTDVPRLLRLFAPRALKEARLRRLQYVGVHMEERLARGILR

DKPDFISYILRSKGTADELTDGEVEANVSFLLLAGTETTATALSGTTYYLLKNPEGLRKATAEVRSAYNS

EDEITFATTAERLPYMQACLTEGLRIYPSGPIAAPRRTPRGTVTWIAGHPVPPVGGASNSPLNFHRQADF

IPERWLSPSTMDPASPFRRDNRAASQPFSVGPRNCLGKAFALNEMQVILARMLWNFDMKLLPQSDGWERQ

RIFTLWDKGPLMVELNEVRSSLHNQLFLQVCMVVCMVVHQSRSLQRAVHSSGPARNLTG

 

>CYP65AA1P Neosartorya fischeri

NFIA_074000

C-term is missing

92% to CYP65AA1 Aspergillus fumigatus

downstream gene is a polyketide synthase

both genes are broken and fused at YNSE/PVEP

PVEP is about aa 410 in the A. terreus PKS gene XP_001215858

The high conservation (92%) suggests an assembly error rather than a deletion creating a pseudogene. CYP65AA1 in A fumigatus is upstream of a short PKS gene on AAHF01000007

MSFLMESSAVGQLSTLNFSNILQALLGS (0)

LVLYAALNSLSNIYLHPLSRFPGPKLWQTSY

IFRHIASIRGTLDVSIKAFHAKYGPVVRYSPDELSFISAEAWKDIYGFRENALPKDPSFYGLIQLSRDKSPSIFTADQDH

HPRIRKALSYAFSEKALRDQEPFVKHYVDLLIQRLRGIADAEDNRIDLVEWYNFTTFDIIGDLAIGRSFNCLQDSAYHSW

VDAFWKSIKISPYARAMASYTDVQRLLRLFAPRALKEARLRHLQYVGVHTEERLARGILRDKPDFISYILRSKGTADELT

DGEVEANVNFLLLAGTETTATALSGTTYYLLKNPEVLRKATAEVRSAYNSE/ begin PKS gene

 

>CYP65AB1  Aspergillus oryzae

          GenEMBL BAE56591.1

          41% to 60A2, 39% to 60A5, 40% to 60B3

          43% to 65S1, 40% to 65A2, 41% to 65B1, 44% to 65H1

          47% to 65S3

          Supercontig 5: 63753-65569 (+) strand

          Revised 3/18/2009

MLFSLGPLTIVYGLVIFVVAKTIYNLYLHPLRSYPGPLLARATRWYYSYYVKIGLLPQKTKELHDQYGPC

VRIAPDELSYNTAEAWEDICGHRTGQRTESFEKDLTFFPPAPNGVDSI

IVAKDDVHRRFRRLLSHPMSDKA

LGSQQEIITGYVDQLIHELRQRSERSEVVDMVRWFNFTSFDILGDLAFGESFGCLGSGLMHPWIELIFTS

IKSVMDMQIIRRIPGLFSLILTIAGLQQKQDLQEQFMFCQKKARERYTKETTRPDFMTYILRATEEKGMT

PEEIEANAQILIMAGSETTASALSGTLFYLLKNSMAMQKLRQEIHATFQAEAEITMRSTQSMEYLHAVLQ

EAMRVYPPVPCTFPRTTPPGGAMVCGRFVPGGYIVGVNQLAAMTSEKNFKDPLKFIPERWCGDERYQEDS

RKAYQPFSYGPRNCLGKNLAYAEMRLVLTRLLWNFEFDLLEESKDWHAKQKVWMMWDKGDLKVRLKPLRH

 

>CYP65AB1 A. flavus

Supercontig 8: 73235-75051 (+) strand

AFL2G_07276 revised

MLFSLGPLTIVYG (0)

LVIFVVAKTIYNLYLHPLRSYPGPLLAR ATRWYYSYYVKIGLLPQKTKELHDQYGPCVRIAPDELSYNTAEAWEDIC (1?)

GHRTGQRTESFEKDLTFFPPAPNGVDSI (0)

IVAKDDVHRRFRRLLSHPMSDKALGSQQEIITGYVDQLIHELRQRSERSEVVDMVRWFNF TSFDILGDLAFGESFGCLGSGLMHPWIELIFTSIKSVMDMQIIRRVPGLFSLILAIAGLQ QKQDLQEQFMFCQKKARERYTKETTRPDF ()

MTYILRATEEKGMTPEEIEANAQILIMAGSETTASALS ()

GTLFYLLKTPTAMQKLRQEIHATFQTEAEITMRSTQSMEYLHAVLQEAMRVYPPVPCTFP RTTPPDGAMVCGRFVPGGYIVGVNQLAAMTSKKNFKDPLKFIPERWCGDERYQEDSRKAY

QPFSYGPRNCLGK ()

NLAYAEMRLVLTRLLWNFEFDLLEESKDWH AKQKVWMMWDKGDLKVRLKPLRH

 

>CYP65AB2 Aspergillus clavatus

82% to CYP65AB1  Aspergillus oryzae

ACLA_066730

MLFPVETLTIVYMLGVFALLKTFYNLYLHPLRSYPGPWLARASRWYYSYYLKRGILPQKTKEWHDRYGPCVRIAPDELSY

NTAEAWEDICGHRTGERTESFEKDLTFFPPAPNGVDSIVTVHRRFRRLLSHPMSDKALNSQQDIIGGYVDQLIHELRRRC

DEPGVVDMVRWFNFTSFDILGDLAFGESFGCLGSGVMHPWIELIFTSIKSVMDMQIIRRVPGLFTLILTVAGLRQNQDLQ

DQFMFCQKKARERCSKETSRPDFMTYILRATEEKGMTQEEIEANAQILIMAGSETTASALSGTLYCLLKNPSALQKLRAE

IHAAFQHESDITMRALQGLEYLNAVLQESMRVYPPVPCTFPRTTPRGGAMVCNRFVPAGYIVGVNQLAALTSAQNFARPL

QFAPERWLGDEQYQHDIRKAYQPFSYGPRNCLGKNLAYAEMRLVLTRLLWNFDLELMPESEDWHSQQKIWMMWDKGPLNV

RIRRLRP*

 

>CYP65AC1  Aspergillus oryzae

          GenEMBL BAE59505.1 AY510452.1

          58% TO CYP65AC2, 45% to 65U1

MSQFAREIVRNAIYNTSSPDADSVSLRKATTTVLLIGVT (0)

YCILVGIYRVTLHPLAKYPGPKLAAVTRLWHSYHLCTGDIVSVLSRAHEAYGPVLRIAPD

EVLFISSRAWDDIYGARPGKPE

MDKDTPLYKGPTAPHSIVTVDGELHRFYRRLLAKGFSDAALREQEPVIQRNINLLVEKLHKEVAAGKTPE

MTAWFNYATFDLIGELAFGETYGCLENSHYHPWVEMILEVMKLRAMTHAVGYYPWIFHILMWFVPKSLRE

KFVTHRRYTHDKVQRRMDQKIHYKDLTTNLVDPQNGLERYEIDGNCSTLIIAGSETTATALSATLYFLTQ

NENAKRKVIGEIRTTFNNAGDINSISVNQLKYLSACMNEALRIFPPGPAVFPRRVPQGGDFIDGHWIPGG

TQVGIAHYCINRSRRNFVDPDKFIPERWLGDPTYQTDDRHAVQPFSYGPRNCIAHNLARLEMRLVLARLI

WEFDWELAPGSERWEEEALVFNVWSTKPLMIKFTPVAR

 

>CYP65AC2  Aspergillus oryzae

          GenEMBL BAE54634.1, AP007150.1

          40% to 60A2 (CYP60 is in the 65 family)

          revised 3/20/2009

MTLSAESSQLPWALGNIVQQVHGIPIWGTVLTILVLVVL (0) HYAIYAVYNITFHPLAKYPGPKVAAMTRIVYTSHLLSGRLVEFLHQTHLKYGAVVGVAPN

EVIFTSSQAWNDIYNVRQGAPE

MSKDTPLYQSLGTPPTIAEAGHDLHRRYRRLLAKGFSEAGLREQEPVIQQKISVLIKQLHAATAKGTTPE

MTSWFNFFTFDLISELTFGESFKCQENGRFHPWILMVTQSIKFRAIIQALGYYPLLFKLFMGLIPKSYQE

AFRDHQKLTSENVQRRIDKKVDYTDLASNLIDPKHNLERYEIDGNCAVLIVAGSETTTTALSATMYYLTQ

NPEAKAKVIKEVRSSFSSAEEITAISVNQLKYLPACFNEAMRKLPPAPAVFTRRVPREGAYIAGNCIPGG

THVGMCHFATNNSSLNFKDPEKYIPERWLGDPEYEDDARAAMQVFSVGPRNCIGQNLARLELRLLLSRVI

WEFDWELDSASMDWDKDMPVYLSWGMKPLKFHFAPVVR

 

>CYP65AC2  Aspergillus flavus

98% to CYP65AC2  Aspergillus oryzae

AFL2G_10525

MTLSAESSQLPWALGNIVQQVHGIPIWGTVLTILVLVVLHYAIYAVYNITFHPLAKYPGPKVAAMTRIVYTSHLLSGRLV

EFLHQTHLKYGAVVRVAPNEVIFTSSQAWNDIYNVRQGAPEMGKDTPLYQSLGTPPTIAEAGHDLHRRYRRLLAKGFSEA

GLREQEPVIQQKISVLIKQLHAATAKGTTPEMTSWFNFFTFDLISELTFGESFKCLENGRFHPWILMVTQSIKFRAIIQA

LGYYPLLFKLFMGLIPKSYQEAFRDHQKLTSENVQRRIDKKVDYTDLASNLIDPKHNLERYEIDGNCAVLIVAGSETTTT

ALSATMYYLTQNPEAKAKVIKEVRSSFSSAEEITAISVNQLKYLPACFNEAMRKLPPAPAVFTRRVPREGAYIAGNWIPG

GTHVGMCHFATNNSSLNFKDPEKYTPERWLGDPEYEDDARAAMQVFSVGPRNCIGQNLARLELRLLLSRVIWEFDWELDP

VSVDWDKDMPVYLSWGMKPLKFHFTPVVR*

 

>CYP65AC3P Aspergillus nidulans 67% to CYP65AC1 AN10101.3 (partial)

ETYGPILRIAPGEVLFTSSSAWDDIYGARPGKPEMDKXXXXX

KGPIAPHSIVAVDGELHRFLRRLLAREFSDVKLREQEPVNQRSINLLIEKLHDEVAAGKTPEMTAMFN

YATFDLIGELAFGETYGX

MENWRYHPWVKMIFYVMKLRALTHAVGYCSWVFPILXXXX

PQVLRD*FVVHRKYTTYDKVQRRKDRKIHYWELTTNLFDPQTGLER

XXXXXXXXXXXXXXXXXX

STALSATLYFLTQNEDAKRKVIGEXXXXXXXX

GDIKSISVTQX

KYLHTRVNETLQIFPPRSAVFPRRVPLGGDWILGGTKVGSVHCCISCCRWNFV

DPDKFIPERWLSAPAY*NDDRRAMQAFKSSSLHFS*SLRLKMRLVLARLICEFDLELA

PGSE*WEGALVFNVWGTKPFKI

 

>CYP65AD1  Aspergillus oryzae

          GenEMBL BAE55230.1

          45% to 65V1, 43% to CYP65AF1

          revised 3/17/2009

MLPANNLAFILSPVVTFELIGGVIAL (0)

VSIAAIASWSYNVFFHPLSNYPGPRLAAATRLWYAWHCAKGSLPFAIHELHLRYGDVVRVAPDELSYIHP

DGWNEIYGHRPGQSEIPKDPSFYSSALSSPEGIFRAPRDRHGYIRRQMSHGFS

EKSMREQEDTIRHYADLMISYLSTQANGPKENVVDFTRWYNYFTFDVMGQLVFGESFNCLQSSGFHPWVS

IIFDSIRYNVFVRCTQFWPWLSPVIRRFIPKSFQRRKIEQQALSREKANYRKTIHDGRNDLVANLLKPDS

GVTDLEYQSTVQTLIVAGSETTASLLCGVTFHLLNNPEKLEKAVKEVRSEFDSADKISFVSVNKLHYLLA

CLNEALRVYPPVADGFPRRTGSNVEVINGQPVPPNTAIRMTHWATYRSPRNFVRPNEYLPER

WLGNAPGFEKDHKNALQPFHVGPRNCIGR

NLAYMEMRLLLALVLWNFDLELYPA SKLWDKQRVYNLWEKPELKVKVLPRKI

 

>CYP65AD1  Aspergillus flavus

AFL2G_00138

MLPANNLAFILSPVVTFELIGEVIAL  (0)

VSIAAIASWSYNVFFHPLSNYPGPRLAAATRLWYAWHCAKGSLPFAIHKLHLRYGDVVRVAPDELSYIHP

DGWNEIYGHRPGQSEIPKDPSFYSS ALSSPE

GIFRAPRDRHGYIRRQMSHGFSEKSMREQEDTIRHYAD LMISYLSTQANGPKENVVDFT RWYNYFTFDVMGQLVFGESFN

CLQSSGFHPWVSIIFDSIRYNVFVRCTQFWPWLSPVIRRFIPKSFQRRKIEQQALSREKANYRKTIHDGRNDLVANLLKP

DSGVTDLEYQSTVQTLIVAGSETTASLLCGVTFHLLNNPEKLEKAVKEVRSEFDSADKISFVSVNKLHYLLACLNEALRV

YPPVADGFPRRTGSNVEVINGQPVPPN TAIRMTHWATYRSPRNFVRPNEYLPER

WLGNAPGFEKDHKNALQPFHVGPRNCIGRN (2)

LAYMEMRLLLALVLWNFDLELYPASKLWDKQRVYNLWEKPELKVKVLPRKI

 

>CYP65AD2 Aspergillus terreus

AAJN01000140.1

60% to CYP65AD1 Aspergillus flavus

ATEG_04967.1

MAISHLLTPDMTDFSLVTALGTVIAL (0)

29486 (0) GVLWTIGSAVYNVYLHPLSRYPGPRLAAASRLWFCWHLIRGELPFVIHELHLKYGDIVRVAPDE  29295

29294  LSYTHPDAWNQIYGLRPGKREIMKDPTFYSS  29202

LPSGKGSIVNAVRERHGHLRKLMSYGFSERALREQESVVRFYADFFHQRDHGDIG &

GKGVRWIWVRWYN (0)

FFTFDVMGHLVFGESFDCLQSTG

YNPWVALIFDSVRIGGLLRSIKFWPWLTPIVQHLVPAKLQERRKEQKRIARQKAAYRKSI

EDGRRDLISSLLQPDSGVTDAEYQSTVESLIIAGSETTATLLAGVTFHLLKNPDKSKRAT

MEVRNAFSSPEEITFVNVNKLPYLIACLTEALRIYPPVGDGFPRNTGENTEVILDERVPPN ()

TLVRVTHWATFHSPSNFARPDEFIPERWLEGEHGFENDRKSAL

QPFHVGPRNCIGRN (2)

LAYMEMRLLMALVLFRFDMELCDVSEDWDK

QKSFLLWAKPELMVRLSPRTV*

 

>CYP65AE1  Aspergillus oryzae

          GenEMBL BAE60010.1

          40% to CYP65AF1, 39% to 65S2

MEALPTYLESFWHSGGGTMGISILVMLSTFLALGTIFVYRIWLHPLSGFPGPKCCSVSSIPVAWAQLRGR

NHEFVSSLHRKYGSVVRISPSELSFISGAAWNDIYSRSKGRPALERDRTFFNDMLVDPETITMANEATHS

RIRRAMAPAFSPRALLEQEPIIQANIKLLMDKLEARAGSGGAPTDLRAWFNYTTFDLIGDLAFGESFGCL

ATSTCHEWVQFVLDHFYTSTLLHVVHRFHPFNRVLAALLPKSLIEKRKAHDSMTLTKVHRRLEVQGRRND

FTQHLLDAAEAGTLSSREVEKQASVLILAGSETTSVALTFAIYLVLTNKPVLDQLNDELHSTFKEEQEIN

LLSVNQLKYLHAVIQEALRFCPPISNGFPRQTPPQGATVDGMFIPGKTVVNINHWAAYRSPRNFTLPEQF

LPERWLGDPRFDEDAKDVFQPFSVGPRNCIGKKFAYDSMKLILAKFLWRFKPTLLDKSRSWLAHQPTFVS

FHQPPLLVDLEIKGSDAFPVRE

 

>CYP65AF1 Aspergillus oryzae RIB40

BAE65144.1, AP007171

CDS             join(1760697..1760719,1761316..1762453,1762525..1762691,

                     1762742..1762901)

MLSFLPLPGPFSSPVGTVGFYLGLGVLL (0)

SILYLFAVIIYNVYFHPLAKFPGPKSYAATRIPYFQALLGGQIGQAIKDLHQK

YGEVVRIAPNELSFIDGEAWKAIYGTRPGHKQKPKDVRYYPPTAGGVPSIVISNDEDHSRFRRTLSHAFS

ETSLRVQEPLVNSYIDLLIQRLHEHCEAGNKPLDMVAWYNFTTFDIIGDLAFGEPFNCLQNSAYHKWVSM

IFSNIRYGTYGNLARRFPGSKFLLRLITPTRIANGRNWHIELTKEKVKDRLAKSNDRMDFYGHILKQKDT

ERAMTFDEMVTNGSTLIVAGSETTATLLSAVTFYLLKNERVLSKLQQEIRASFESEKDITVTGCNQLEYL

NAVLTEGLRIFPPTPTGLPRIVDADGDMIAGKWVPGGTIVSIPHLAAFHSASNFTEPESFIPERFLGDPR

FANDSKTVLQPFSFGPRNCIGRNLANAEMRLILARVLYNFDLELDERSENWSRQETYILWNKPGLY

VRLRPRVGI

 

>CYP65AF1 Aspergillus flavus

98% to CYP65AF1 Aspergillus oryzae

AFL2G_05426

 

MLSFLPLSGPFSSPVGTVGFYLGLGVLL (0)

SILYLFAVIIYNIYFHPLAKFPSPKSYAATRIPYFQALLGGQIGQA

IKDLHQKYGEVVRIAPNELSFIDG

EAWKAIYGTRPGHKQKPKDVRYYPPTAGGVPSIVISNDEDHSRFRRTLSHAFSETSLRVQEPLVNSYIDLLIQRLHEHCE

AGNKPLDMVAWYNFTTFDIIGDLAFGEPFNCLHNSAYHKWVSMIFSNIRYGTYGNLARRFPGSKFLLRLITPTRIANGRN

WHIELTKEKAKGRLAKSNDRMDFYGHILKQKDTERAMTFDEMVTNGSTLIVAGSETTATLLSAVTFYLLKNERVLSKLQQ

EIRASFESEKDITVTGCNQLEYLNAVLTEGLRIFPPTPTGLPRIVDADGDMIAGKWVPGGTIVSIPHLAAFHSASNFTEP

ESFIPERFLGDPRFANDSKTVLQPFSFGPRNCIGRNLANAEMRLILARVLYNFDLELDERSENWSRQETYILWNKPGLY

VRLRPRVGI*

 

>CYP65AG1  Aspergillus oryzae

          GenEMBL BAE60004.1

          45% to CYP65AF1, 39% to 65U1, 47% to 655Z1

MSRRFPIQVAQSEIPPIAWGLWRMVYRLWLHPLSGYPGPRLAAVSNLPYFAWTCTGNLHLRLQELHKVYG

DVIRIRPNALTYRTPEAWTDIYGHRKPGTLPFSKDPEFFMPAQAGSSHMINANEKDHTRQKRLLNHAFSE

RSLRQQEHLIMGYIDLFIQRLRGQARMGAETVNMEEWLNFLTFDIIGDLAFGEPFGCLQNSEYHPWVATI

FKSIKTGAILRALNIYPILLGFIRRFLPKSLVQKRIAHYQMSKDRVTRRLQTETSRPDFISYILKYNDDR

GMSTPEIEMNAALLIQAGSETTATVLAACLYFLQKNAACHRRLVQDIRSAFTQETDINFLSAAQLPYMNG

VIEESLRLFPPAPGIGPRVVPKGGARICGRYVPGGVSVSVGHYSTFRSARNFTRPNEFLPQRWLDRDAES

EFASDQTMALQPFSYGPRACIGRNLAYAEMRTILAKILWHFDVQLDERSADWANSKSYIVWEKGPLWLKL

HPRNVPQETD

 

>CYP65AG2 Aspergillus niger

e_gw1.3.1457.1|Aspni1

57% to CYP65AG1

MDGPIPKLLVSVSRTLIFKYARPLHHPQSSSQLPIASPGPNRILEKVIIVTIFRYFYRLFLHPLRSYPGPRLAAISHLPY

ISWCVRGILHSKIRTLHEQYGEVVRIRPNSLTYCAPQAWEEIYGHRKKGQRTFTKDPQFYIASSGQFTSMINANEEDHIR

QKRLLTHAFSEKALRDQESLIMAYIDVFITRLAEYAASESPRATNIVHWLNYLTFDIVGDLSFGEPFGCLDRSTYHPWVE

TIFKSIKTGAFLRALAIYPLANIAFNKLKPKRLIRKRLEHYQLSRDRVHKRLHVSNTISRPDFLKYILKNNSFQEGAGMA

VAEIELNAALLIQAGSETVATALSACCFYLGRNPPKLEATVREVRTTFADSGHISFSATSALLYLNAVIKEALRLYPPAP

GIGPRLVPEGGAVINDQFVPAGVSVSVAQFSTFRSPASFIEPDSFLPERWLPNSDSRYLTDNKNALQPFSYGPRACIGKN

LAYAEMRTILSKILWHFDISLQPGSDSWDQSLSYIVWENQPLNVVLRPAMNS

 

>CYP65AG3 Aspergillus terreus

64% to CYP65AG1

ATEG_08791.1

MAGWNILLTTAIAGLVCRIVYRVLLHPLSKYPGPKLAAISNLPYTKWSVTGQLHSRIRELHERHGDVVRIRPNSLTFSDP

QAWTDIYGHRKAGAPPFRKDPEFYIPSSNGSTNLINSSEADHARQKRLLTHAFSERSLRDQESLVMAYIDLFITRLGELA

NNSQDVDLLHWLNYLTFDIVGDLAFGEPFGCLKDSAYHPWVAILFQSIKTGALLRALTIYPTLAKIIRYFMPKSLLRKRA

DHYRLSKEMVTRRLDMDTTRPDFISYILKYNDERGMSRSEIEVNAALLIAAGSETTATTLSACVYYLQKNPECYKALTEE

VRQAFSKDEEITFLAAGKLPYLNAIIEESLRMYPPAPAIGPRLVPEGGAVVNGEHIPGG (0)

VSVSVAHYPTFRSSSNFAEPDSFLPERWLRDNEDSRFRNDKREALQPFSYGPRACIGRN (2)

LAYAEMRTILSKLIWHFDITLDPRTTDWDNARSYIVWENKPLWVRLTPTSAHLSSK*

 

>CYP65AH1  Gibberella moniliformis (also Fusarium verticillioides)

          AF155773.4

          fumonisin biosynthetic gene cluster,

          FUM2

          47% to 65L1, 45% to CYP65R1

also Fusarium verticillioides FVEG_00323

MYQITAICDARWWLCLAFTCMAMLALFSFGVVMYNIFFHRASKF

PGPTIAGATSFRYHWAMSTGNVAPWLREQHARYGEVVRIAPDMISYVSPDAWKDIYAY

KPGEKEQNGIDWTIPSRDDDVPSMFSEPNDAEHNRVRRLFLPAFSDRALKQQEPLLSK

YSDQLVHLIRRGIDDNRDQEFDAVKLYNFTTFDIMGDLTFGEPLGLLKNSSYSEWVQN

LFRDIKTAGIFLFIFDFPPLPWLVKKFSPPSIQRAHEIHKQHTVDRVNRRLEKGLDRP

DIWNLVLSQPEGRGLTHPQMHANADIFMIAGTETTATLLSGLTYLLLKNPEKLQRLVE

EIRGSFGSIEELTVENLARLPYLSACLSEGLRCYPPVPIGPSRVTPKTGGQVLGERVP

GRVRLTIPQCAAYYSDLNFKDPYSFIPERWLPGTGYESDRKGILQPFLIGPRNCIGQN

LAYHEMRLILCKLLWHYDIELCPDKGNWLLDQKMYIFWEKRPLMVKFTKARK

 

>CYP65AJ1 Alternaria solani

Alt1 BAD83680 48% to 65J1 Mg

PKS for alternapyrone is next to three P450s called alt1, 2, 3

Chem Biol. 2005 Dec;12(12):1301-9.

        1 mpaaselerr tkrsksrnki skpianmlsv iafsicispi vyflirsiyy lvfhplsdyp

       61 gpklwaisri pwnyvnlqgd lawrirdmhl hynssviria pdelsytsst alkkidgtpp

      121 preflkcldg rgiapavvnr rrsivtetpe rhtilrralq pafseralrd qedffrdhtd

      181 rliaqlrkpq ygvteqnilr wfallsfdim sdlafgqpag cldlvdepwl gvigsrvksi

      241 vwyqfavyyr iewilkwimp kaamearkrh qaltlqkvqr rieeersgkr egkkrdfmsy

      301 ilgndkenls nmdlfgmasa fivagsnttt ytmtaftffv crdsevyakv iaevrdkfas

      361 dtditmvaag dlpylkacie etmrlspptp salprwvleg geeidgkwvp ggvtvgvhnl

      421 aachvpwnwh rplefiperw lqtkegefth ddralharfl mvdimsltcf lssmamnemr

      481 lalaklfwnf dislsrnsgn wwitqksylv wekkplmvti kprh

 

>CYP65AK1 Aspergillus niger

fgenesh1_pg.C_scaffold_9000016|Aspni1

42% to CYP65R1, 34% to fgenesh1_pg.C_scaffold_5000711

MIHENLHEALQGRYLILTAGLLFFSLVGKCFYELYINPLCKIAGPKLAAVTRLVYMYHNLMGDEHTWMRNLHLVYGDTVR

ISPDQVSYSSAEAWRDIYGHASATKKSTEKDKRFFGTTLNGTPDVIRAPAADHSRFRRNFSHAFSDKALREQQPLIAHYA

DMLIDKLREIVQADPKARIEMVMMYNLTTFDIMGDLTFGDPLNLLKGTGNVAWVTSIFSSVKINSLRRIARYYPWCSYFL

NSLLPRSLKQRQASHYQSCKDRVDWRVDQSLDRPDIWGLVMAQKESLRLSREEMYANSQLFMVAGTETTATALSGLTYQL

LLNPDKLEKITNEVRDAFEQSSDIDMLRLAQLKYLNMCIDEGLRMYPPVPTGMPRLTPKEGMEICGEYIPGDTAVSVHHW

ATYRNPRNFTRPDEFLPERWGNDPQFASDNKAACQPFSFGPRNCLGKNLAYHEMRLILAKVLYHYDLRLAPESVGWEKQN

TWTLWQKNRLMVQLTPRA*

 

>CYP65AK2 Aspergillus terreus

68% to CYP65AK1 Aspergillus niger

ATEG_04213.1

MFVEALSQVSFEHLPHIVLGTVCVPIPPVHGLQLTSVHSLQLVFAAIAKITYELLINPLRKYPGPKLASVTRAPYIYHTI

KGDLVQWIHNLHTVYGDVVRTAPDELSYATGDAWKGIYGHASAGKKATEKDMRFYGPSFNDTPDIIRASGPDHARFRRNF

SNAFSDRALREQQSLICRYVDMLIEKIKVEIEIDPLAKVEMVRLYNLTTFDIMGDLTFGDPLDLLKGTGNTGWVSSVFSS

VKRGAFRRASRYYPWAALLVKLSVPRSVIEKAANHFRACVERVDWRVNQKLDKPDIWGLVLDQKENLRLTRAEMHANSQV

FMLAGTETTATALSGLTYQLLLNPDKLERITKEVRDTFKQDSDIDITTLAQMKYLNACIEEGLRMYPPVPAGLPRLTPPE

GMVICGEYVPGKTAVSVAQWATYRNPKNFKRADEFLPERWTDPSFSTDDKSAFQPFSFGPRNCLGKNLAYHEMRLILAKV

LYNFDLVVAPELVGWDKQKSFTIWEKNDLMVQLKPRD

 

>CYP65AL1 Aspergillus niger

fgenesh1_pg.C_scaffold_5000711|Aspni1

42% to CYP65Z2, 43% to CYP65Z3

MEVGLFQTMVANANGLILAVLVSLGCWVIYTLYFHPLARIPGPKWWIISRIPYFRSITTGQFIHSVRALHERYGPVVRLA

ANEVSFTDPQAWQDIYGHHGGERAFPRNPVWYQPALNGVHHILSASNSDHARIRHLLSHAFSERALKSQEPMIQVFFNLL

IERLRPLADGRQAININDWLQYTTFDITGELEFGESFGCLEKGEYHPWIKVLLSNFRKIILFGASQLIPGLRFLVGLTIP

KAVMQQRQQHFSYTVEKVSRRLARGNDPMRPDFLTYINRYNEDEKKGMSRAEIDSTFNVLVIAGSETTSTCMTATLHCLL

RNPATLQRLQTEIRSTFAHDSDITFDSTIPLPYLNAVIEESLRLCPPAPTMLPRVTPAAGAYVCGYWLPGGTAVEISQYA

MSRSHANYGDPDRFIPERWLVDEKPNLHPHDARAHRPFSFGPRNCLGRSLAWMEIRCVLCKLLWNFNLRMESPDYEWNEQ

GTYVMWDKKQMLISLTPVKR*

 

>CYP65AM1 Aspergillus niger

estExt_Genewise1.C_31719|Aspni1

45% to CYP65AF1, top 28 hits are CYP65 or CYP60

MAINTLVMDVAMRVEWLATPRVILGIGAGWLFIYIISKVVYALCLDPLRHIPGPRLNAVSMIPYARHLLAGTTVQNSVSL

HQQYGEVVRIAPNEVSLICGETAFPDIYGFRTGKMKGHLNMSKDPVWYVTPSNGAPSILQANDEDHSRGRRVLAHAFSDK

ALMGQEPLVQRYVDQLIDRLKEVTSANDEPVDMVKWYNWTTFDVIADLLFGEPFGCLQDLSTHQYVALLFQSLKSLRMIY

ILAYYPWLRFLGNLIVDKGLIQKRKEYATWVSTQVTKRINTATPRPDFMSHILANNNNRGVKLTRAEIDSNATLMTTAGS

ETTATLLSGATYLLLTHPRVLRTLQDEIHSEFPTYESITLEAVAKRTPYLNAVISEALRFFPPIPVGFIRRVPKGGETVS

GYWIPEGTILSVSHYAAYHSERNFRDPDSWVPERWLGDERYVNDKRAAFQPFSFGPRSCLGRNLAYAEMRLILAKMVWSF

EMELDEGLSRDWLQRCRVMRLWQKPELEVRVRPR

 

>CYP65AM2 Mgr059 Mycosphaerella graminicola

65% to CYP65AM1 A. niger

MDSLRDRLAGKPWTIVGLLVALAAAYFVVTAIQHVLFSPLKHIPGPRINALSRIPYIRHMLAGTTAKMTVDLHKQYGEVVRISPDEVSFISGETAWGDIYGFRTGKLKGHLNTQKDPAWFPPPVNGAPSILLAKDEQHSKGRRLLSNAFSEKALTEQEPLVNRYVDQLVDGLRDTIRTEKKPVDMAQWYNWTTFDIIADLLFGEPFGCLQNKETHKYIAMLLEGLKAIHFTYIFRKFPLAKHFMGLLVDKKTIPSPNVRVNYQKWISSQVQKRIDRETQRPDFMSHILANNGEKGYEMSRAELDSNAQLFINAGSETTATLLSGATYALCRNPRVLEKLKKEVRGRWNNYHDINVAEVSKAPYLAAVISEALRYFPPVPAGFNRKIPEGGEVVSGYYLPGGTSTTISQYAAYHSEMNFKDPDAFIPERWLDDPKYANDKRSACQPFSFGPRNCLGKNLAYAELRLIVAKMIWTFDMELDAASNDWLERCQVFTLWWKPALLVKLTEAVRD

 

>CYP65AM3 Aspergillus clavatus

92% t0 65AM1

ACLA_077670 MAIGTLVMDIAMRAERLATPRAILGIAAGLTFIYLLSKVIYALCLDPLRHIPGPRFNAVSMIPYARHLLAGTTVQNSVSL

HRRYGDVVRISPNEVSLICGETAFPDIYGFRTGKMKGHLNMAKDPVWYVTPSNGAPSILQANDEDHSRGRRVLAHAFSDK

ALMGQEPLVQRYVNQLIDRLKEVTSANDEPVDMVKWYNWTTFDVIADLLFGEPFGCLQDLSTHRYVALLFESLKSLRMIY

ILAYYPWLRFLGNLIVDKGLIQKRKEYATWVSTQVTKRINTATPRPDFMSHILANNDTRGVKLTRAEIDSNATLMTTAGS

ETTATLLSGATYLLLTHPRVLRTLQHEIRSEFPTYESITLEAVAKRTPYLNAVIAEALRFFPPVPVGFIRRVPAGGEMVS

GYWMPEGTVVSVSHYAAYHSERNFGDAESWVPERWLGDERYVHDKRAAFQPFSFGPRSCLGRNLAYAEMRLILAKMVWSF

EMELAEGSRQDWLQRCRVMRLWQKPELEVRVRARVVA*

 

>CYP65AM4 Mycosphaerella fijiensis

62% to CYP65AM2 Mycosphaerella graminicola

estExt_fgenesh1_pm.C_10205

MDTSSAERPISFTAGFATLALLYVVGNLIYKLYFGPLSHIPGPKLNALSRLPYIRHLLKGTTVQNVNELHEKYGEVVRIS

PNEISFTSIDTAWPDIYGFRTGKMKGHLNTLKDPAWYPPPMNGAPSIIIADDDLHSKGRRVLSHAFSEKALTDQEGLLQG

YVDQLVDGLRETIKTKPVADLTKWYNWTTFDIIADLMFGEPFGCLQTGETHWVITLLFDSVKAFRMFYISRYWPWVKSLG

SMIIDKRLIAKRTVYFAWVSKQVKNRMERDTQRPDFMTLILQHNGEKGYKMEKGELDSNANLMLTAGSETTASLLSGATY

CLLTNPAVLQKLTEEVRGKWASYDEITLHEVNSSPYLVAVMNEALRYFPPVPTGFERRIGPGGETVSGYYIPEGTSVCVS

QYPAYHSEKNFKNAEQFVPERWMGDEEYKDDKRSGMQPFSFGPRNCLGKNLAYAEMRLIMAKMVWSFDMELELQSRDWIQ

RCRVHTLWDKPALMVRVTEAVR*

 

>CYP65AN1 Aspergillus niger

gw1.16.109.1|Aspni1

36% to CYP65Z1 top 26 hits are to CYP65 or CYP60

YSILCVFYNIFFHPLKHYPGPRIMASSRLPIFFLVLIGRSVNILEKLHAKYGPVVRVAPDELSYTAASAWKDIYSSSAAW

PLAIPRNMVFFRAMAGEYGFHSLVTANNQDHARLRRLYSRAFSKQALAAQEPLIIQHIDNLIKKLYAEIDHTAKPVDINL

YLNLALFDMINDLQFGEPLNLLDDTTHRAFARASLIIVPSAAVIQALADFPLARIILKPILRELFRMRRLYFSTTDERLE

KRLASNSYRADIVQFLTDAKSDNISLQEIQANAPLMNIAGSGTSAVALSGLIAHLLLAPHILKDLQSEIRSKFHTTSNIT

MKNMVDMPLLDACIKEVLRVFPAVPVTPGRLVPSPGVTIAGKWVAGGTRVYVTPLAAFHSTDNFHDPETFAPQRWYNTKE

ESSFTCKDVKGAYKPFSVGPYNCIGQYMAHYVVSLFICNLLLHFDLEIATDPKTWLSNQRAWIIWHNPPLNV

 

>CYP65AP1 Aspergillus niger

gw1.10.1058.1|Aspni1

40% to CYP65AF1, 60% to fgenesh1_pg.C_scaffold_1001144

Top 27 hits are CYP65 or CYP60 (CYP5118 also in this set, may need name change = 41% to CYP65Z2)

FPILLALKRLYFHPLSRYNGPLLWALTRVPYMLAFRNGQLAHKIKAFHEVYGETVRVAPNEVSFINPDTVKDIYQRRPSG

GGFKSLPKDPIRQAPPRPGQPISILDAGDADHTRLRKAYAAAFSSQALSAQEPLVASYVDKMITQMQCQSKLIAQSVDLQ

EWISFCTFDIVCKLSFGEDFGCLDNQRYHEWVGQLVHSLKAKVQLASCRFYPWLFNYLVKRLPKSAGILMAQHQATTKEK

VKRRLQQLESDAKSSGAAGVIRPDFLAHLVQQSRQEISEGEMVVNAATLIVAGSHTLQTAITGILFHLLQNPTVLNRVTS

EVRGEFPSTSQINVSTLLRLPMLEAAIKEGIRLTSPVPLGLTRLVPIGGHTINGEYFPEGTVVSYMQWAANVSPQNYTDP

QAFHLERWLKSRQPSSSSRDDFFEKDQRHATQPFLQGPRDCIGQNLARMEIVLILGKLLYHFDLQPEGGLGHWEDQETYA

VWVKTPLPVKLRVRQTD

 

>CYP65AP2 Aspergillus niger

fgenesh1_pg.C_scaffold_1001144|Aspni1

40% to CYP65Z2, 60% to gw1.10.1058.1

MLAFRSGRLAHDIKRFHDIYGDTVRVGPDEVSFLDPDCIKDIYSRRPNNPHYKALPKDPIRQKPPQPGQPGDILDVEDDD

HSRIRKAYAPAFSTQALNAQKPLVTSYVCKMVAQLKSRANSTDPSRRIVDLQRWVTYCTFDIICSLSFGEDFGCLDNDSY

HEWVGMVVNSVKGKVRIAAFRFYPWLFNYLVKSLPKSAQLMVKRHQAMTNEKVQKRLHTAVDRPDFFSYRLKSKNELTEP

EMEINAATFIFAGSHTLQSSLTGILFHLLKNPEALARVAEEVRSSFPDEGDLDANKLSKLPLLDAAIKEGIRLTAPVPLG

IPRLVPRGGHTVCGQYFPEGSKVAYNSWAAGVSPRNFADPTKFHLERWLNPDEGRFANDRRSAVQPFLQGPRDCIGQNLA

RLEMSLVIGYLIYHFDFVLPNGREALGEWEDQETYAVWMKSPLMVELVAR*

 

>CYP65AP3 Histoplasma capsulatum G217B

ABBT01000035.1 Ajellomyces capsulatus G217B

68% TO CYP65AP1

HCB00649.1

MTVNALFTAVTTALLLPILIALKTL

32798 YFHPLSRYNGPTLWAITRLPYMLAFRSGKLAHKVKHFHEVYGDTVRVAPNEVSFI

NPSCVKDIYNRRPSSEFKSLPKDPIRQPPPQPGQPISILEAGDEDHARIRKSYASLFSSKALAAQEPLVSSYVHKMISQL

KLQTGESNTTVVDLQTWVSYCTFDIICSLS FGEDFGCLENNRYHEWVGMLVHSLKGKVQLAACRFYPWLFRLLLKRIPKS

AMSLMAKHQATTREKVDRRL

SSTVDRPDFLSHLQNSKHQLSHAEIVTNSTTFVFAGSHTLQTAITGILFHLLHNPESLAK

VTEEVRASFATTEDIEFRNLSKLPLLDAAITEGIRLSSPVPLGLTRLVPEGGHTINGEFFPAGTIVSYMQWAANLSASNF

ADPTSFLLDRWL HTDRDLFAN

DRRDAVQPFLQGPRDCIGQNLARMEISLILAHLLYQFNLSPPTGAGHAGVQRWEDQETY

AVWVKSPLPVRLSVR 34272

 

>CYP65AP4P Aspergillus terreus

some parts are 76% to CYP65AP1 Aspergillus niger

ATEG_04921.1

LYFHPPSRYNGPLLWALTRVFYMLAFRSGQLAHKIKQFYEQYGD

TVRVPNEVSFINSDCVSDIYS

LVTGHVGKTIVQLKTRAAAATPVDLQRWISYCTFDVICSLS

FGEDFGCLEHNRYHEWVGVQVYSLNAKVQLAACRFYRWLFRLLVRSMLASAQVAMAKHQA &

PVFNAAI

KEGIRLTSLVLLGLTRRAPSGGAIFCGAYFPEGTVVSYMQWAANVSAKNFTDPTEFTLERWLPPVGPRYQHDRTDATQPF

LQGPRDCLGQNLAKMEMALTVGRLLYEFDLSVPAG &

WEEQETYTVWVKSPLGVQMKGVVM*

 

>CYP65AQ1 Aspergillus niger

gw1.10.1062.1|Aspni1

37% to CYP65Z2 top 36 hits are CYP65 or CYP5118A1 (needs name change)

PTTLVIYNLFLHPLARYPGPTLWRAFRFPFLLANIGGQLPHRIHDFHAQYGDIVRVAPDELSFVDPRAWRDIYTSDREFV

RPHQYKDQPPGKTAANLIACSEAEHARLRKNLAPGFSEKFAALQEPILQKYIDTLFAKLDAKIASSEGDSASKGADVDLV

EWINYLAFDVIGDLTWGSSFGCLEGLRYHPWVQTVSQFKAAIVVGMIKFYPPLYRLLMAITPADALKPVMEMWKVTDERV

AERVATTAPSTTTRPDFVGIMMASGAMTQEEMEVNSMLIIAAGSESITTVITGVINYLLREQDQRSLRELVNLLHNTFPT

EEKITATRLKSVSYLDAVLMEGMRLCPTIPDAMRRQVPRGGAKVAGQFVPEGMIVSIPPLASYRAPGNFSSPTEFVPRRW

LGEDKQFDLSKQKKAFNPFSLGSHNCPGQNLAWIELRLILARLLWRYDLAAVDVPAWEKQGIWWFWDKKPLVVRIK

 

>CYP65AR1 Aspergillus niger

gw1.11.201.1|Aspni1

36% to CYP65AH1, top 21 hits are CYP65

MVTYNLFIHPLKEVPGPRMLAASRIPVTYASLKGIQTQWLHNLHLKYGPVLRVAPNELSFIDPQAWKDIYSSSPTEPQSM

RRGSDFFRYLEDDDNRPSILAANEIDHPRIRRAYVPAFSRRALARQESILAKYGDALVERLSGIEGQITNIRNMFHYTLF

DIVGHLHFGEELGVLRTGAHKSWVHSQHRLVRAATLLSALAEFSWINLLFRSTIPWMSKWLRKAYFKPSNDLIDRRLQLQ

SDEPDLIGLAFRNSRSLSLTEKDIRANAHIMMTGGAETTTALMTALTAYLLQHPECLARLKNDIRTAFHDKSQVSLESVS

NLAYLDACIKEALRLYPSVPGALHRATPAGGARIAGRWVSGNTRVYIPIFAATHSPTHFWSPDKFIPDRWLDGADDPYQK

DNKTAFHPFSTGPRGCIGQDMAYAMTKIVFCKFVTEFDCELAVNESSPWLAGHRSWTTWEVPPLMVRVSRCK

 

>CYP65AS1 Mgr044 Mycosphaerella graminicola

39% to CYP65E1, all top hits except CYP5117A1 are CYP65 sequences.

This may mean that CYP5117 should be renamed.

N-term from JGI model estExt_Genewise1Plus.C_chr_11525|Mycgr3

MLDWMSVAPSGLSAFAGTVLLAL

IYSATTVGYNLYLHPLAKYPGPKVRAASGWPYFLDLIRGNSPQYMLELHDQYGPVVRVAPNELIFNRPQAFKDIYGRKAG

QKELRKDEMYASGMGEPTLLYCDETYHSYIRKLMAPGFSESALRKQETVVQSRLALLICKLHEEGQDGHASIDLMKWVNF

FIFDVVGHLTYGDSFECITSSALHPWITKFTGLGRPMAYAQASQRLPRFLRLPFLALMLDRSLLSDRKFLYKTSEAKVQA

RIAKESPIPDFMGTLIESHKDGKMTALQLNSNAVFLMAAGTETLVTSVVHTIFRLLTNKHALLKLTQEIRSTFTTSEDIT

MTGVNKCRYLLACLDESLRIQAPSPVTHPRFTPTEGITIDGHFVPGNMAVGVPIHAACRSSVNFHDPDTYAPERWTGEDR

RYINDSKDAAMIFSVGPRDCLGRSIAMIGLKLVIARLIWHFDIEQCFPDDWAEQKVYLVWEKSPLMVKLHPVK

 

>CYP65AT1 New seq e_gw1.3.1373.1|Mycgr3 Mycosphaerella graminicola

40% to 65L1, 41% TO 65Z2

MSPYLILAVITATYLGFKIVRSLHDAYFGPLSKFPGPKLRALSKWPSNITVIRGSEGVELPALHEQYGPVVRIGPKELSY

AGGAASWRGIYGWANNPRSCPMYADFRRTKPLNRVDGIFTADDANHGRQRKILSHSFADKTLKDFEPMLKLWVGKLTDRI

ADKSGEEVDLLQYYNCTTFDIMGDLTFGEDLHMLETGEYNDWVKAIFQGIKSGTIIRGVKHLSAISEWVVNRFVVYNPKV

RTATAAPSFANVCSLYSERRDLWTKILAKSNTPEALTYDEHHSNAAIFMVAGTETTAAALAGTTYWLLRNPDCLNKLTSE

IRDAFANAAEITLDSVQRLQYLQAVLQEGLRMYPPVPSKLPREVPAGGTMIDGELIPEHTSVGVHQLSTYRLESNFKDAY

EFHPERWLGDERYANDHLDALEAFSVGPRSCIGKNLAWHEMRLILTNILFHFDLQLSKESIDWHDQKIYTIWEKRPLLVR

AVPRSS*

 

>CYP65AU1 Coccidioides immitis

47% to CYP65X1

CIMG_09823.2 revised

MELLSSILLFLGLAAVLGVLKIGYNVIYNLWLSPLAQFPGPKLWAISAIPSQLSIVRGTIHFDITALHERYGPVVRIRPN

ELAFNTAQGFKDIYGFRPGGCFPKDRSRYMTPINGADHLVSAIDDGVHARHRKLLSYAFSNRALREQENVIRGFTDTMITKVRGEVHAGRPTVDMKNWLNYTTFDITG

DLMFGESFNCLRDTK

LHPWIAMIFDSMKALAIMGAAGQFPLVQPWLDKLIPAYLKKQEFDHFNLSAGKADRRLESGVSPRGDFMSAFIQNGFVEK

QEQFYEGKKILRRDEIHSNSFILMLAGSETSATLLAGCIYFLCKSPETMQKLSDEVRSAFTSDSDITFDKAATLPYLGAV

LEEAMRCYPPVAAFLARVVPPGGASVDGYFIPENTIVACHHYASYHSSSNFSRPNDFIPERWLGDPRFESDKRDAMQAFS

LGPRNCIGKNMAYAEMRMILCKLLYHFDMELTPETTDWTNQKVYFLWAKKPLMVTMKDRLSSDY

 

>CYP65AV1  Histoplasma capsulatum

39% to CYP65AZ2, 40% TO CYP65AF1

HCB02537.1

MGFVDSYITLTIFGVGLLLYVVGNAIYNIYFHPLSKFPGPKYLAVGRIPITLATLRGQKAQFRFNAHRKYGEIVRIASNE

LSFAHAQGWRDIYGTQAKLQMAKPASGIEEQEGAQSVVTAEGDTHVRQKRMLATMFTERMMKEKESLFISHADLLVKRLA

MLEGQPVALGDWYNFTTFDMMSDLLFGESLGMLSNSEYVPWVRSIHGFLKAFAFITVLNEYLWFRVLWAFFPSKILNTLR

ETHFLFTSQKVEHYLQLDEKTRAGMMGHLLDGGSRKGLNLGELHTNAPILIFGGSETSATQLRSLSFLLFKNPEKLGKLI

SEIRSAYTSSDRITYDSLLEHTYLNACIEEGLRYYPPCSNGLPRVVPKGGTTICGEFVPGGTNVSVATYALFRSPKYFTQ

PDSFIPERWLRESEGGSPVFATDQKGIALPFSYGPHNCPGKKMGYFELRLILAKVLWHFNLELLPEADGAEDSWDNMQNY

QTWSRRPLKVSAKPVRR

 

>CYP65AW1 revised Fusarium oxysporum

44% to CYP65A1 Fusarium sporotrichioides

FOXG_17343

MSRLVGFLINARSPSTCIFLLFAS (0)

LVLYLIGKITYNVFFHPLRKFPGPILWSMTQIPFSLA

WTSGAGHKKIYDLHQIYGDIVRVAPNELSFGYPEAWEDIMGHRKRGQAENGKD

PDFWRGNDIYTLVGSDRDRHSRLRKILSHGFSAQAMMEQQPIFQRYVNLMMKKLREASSSGQSVEMTQWLNWATFDMAGD

LIFGESFGCLEKVEYHSWVKLLYKHIEGFAVSTALIRYPFADTIIKLMTPKHVARDIKAHSDFTKAQVGKRMAYENPRPD

FMESMIRAYEKGHVNHAELLANAHNLIIGGSETTATTLAGTIYLLATHRPILMKLYAEIKERFRSEEEIDLLSVQKLEYM

FAVLHEGLRVYPAVPAAIPRKTSEAGMIGKHYVPANTIVSIWPWPMFHNPKFFKDAEVFVPERWLGDPKYADDKRVAVQP

FSVGPRNCIGKNLAYAEMRLILAKLIWNFDIDLDSRSEGWLGKNTAFLLWEKPELWVRLTPRSDI*

 

>CYP65AW1 Fusarium verticillioides

90% to CYP65AW1 Fusarium oxysporum = ortholog

FVEG_14125

MSRLLAPLVEFRSASFFISILFAS (0)

LLLYLVGKIIYNVFFHPLRKFPGPILWSMTQIPFSLA

WTSGAGHKKIYDLHQIYGDIVRVAPNELSFGYPEAWEDVMGHRKRGQAENG

KDPDFWRGNDIYTLVGSDRERHSRLRKILSHGFSAQAMMEQQPVFQRYVNTMMKKM

REASSGGQPVEMTQWLNWATFDMAGDLIFGESFGCLEKAQYHPW

VKLLYKHIDGFAISTALIRYPFADTIIKLMTPKHVAKDIKAHSDFTKAQV

GKRMAYENPRPDFMESMIRAYEKGQVNHAELLANAHNLIIGGSETTATTL

AGTIYLLATHRPILTKLYAELKERFQTEEEIEFLSVQRLEYMFAVLHEGLR

IYPAVPAAIPRKTSEAAMIGNHYVPAN (0)

TIVSIWPWPMFHNPKFFKDAESFVPERWLGDPKYEDDKRVAVQPFSVGPRNCIG

KNLAYAEMRLILAKLIWKFDIDIDPRSEGWLEKNTAFLLWEKPDLWVRLTPRSDL*

 

>CYP65AX1 Fusarium oxysporum

42% to CYP65B1

FOXG_17420

MLSVSILAGAVAATTVLCIGYVVYNIFLHPLRNFPGPLLCRASPLYRHYKFLRGDLLFETQRLHEQYGPVVRIRPNELSF

IDPEAWRDIYVAHGGSARLGDMARYDRFYQWAGPSAPETFVSLNRPYHDSMKRQLAPAFSERSLQFQEPIIQGYTDTLIR

KLTEVSKDGNPVNLREWFNYYTFDIIGNLGFGSDFGGLESEQYHPWVKAVSQNVREFAFMQVLMYLGLQRIVHVLSGSSL

LRGKILHEHLTREKVEARIKVEKPRLDIFQPLLDRKPPLTFAQLMENSTILITAGSESSVTLLVAAVSLLTDNPAKLQKL

AEEVRSTFNNDDEITIVSVNQLTYLAACLNEALRCFPAVPPALPRVVPHGGAVIAGHAVPEKTVVAVASWATNHSERHFK

KALEYHPERFLKDPEFSKDRFEAFQPFGLGHGNCPGRNLAWAETRLALARLVYNFDIESVPESRGWTTKQKAYMLWDKKP

FWAVLKPVR*

 

>CYP65AX1 Fusarium verticillioides

94% to CYP65AX1 Fusarium oxysporum

FVEG_12501

MLSVSILAGGVAATAVLCIGYVVYNIFLHPLRNFPGPLLCRASPLYRHYKF

LRGELLFETERLHKQYGPVVRIRPNELSFIDPEAWRDIYVAHGGSARLG

DFARYDRFYQWAGPSAPETFVSLDRPYHDSMKRQLSPAFSERSLQFQEPI

IQGYTDTLIRKLTEASKDGKPVNLREWFNHYTFDIIGNLGFGSDFGGLES

EQYHPWVKAVSQNVREFAIMQVLMYLGLQRIVHVLSGSSLLRGKILHEHLT

REKVEARIKVEKPRLDIFQPLLDRKPPLTFAQLMENATILITAGSESSV

TLLVAAVSLLTDNPAKLQKLSEEVRSTFKNEEDITIVSVNQLPYLAACLNE

ALRCFPAVPPALPRVVPHGGAVIAGHAVPENTVVAVASWATNHSERHFN

KALEYHPERFMKDPEFSEDRFDTFQPFGLGHGNCPGRNLAWAETRLALARL

VYNFDIESVPEGRGWTTKQKAYMLWDKQPFWAVLKAIR*

 

>CYP65AY1 Aspergillus flavus

40% to CYP65S4 Fusarium verticillioides

AFL2G_11804

Note: there is no ortholog of this gene in A. oryzae

MAARLLSSVSLTDVVLLLSSVWIAVHLVLAAYNVYLHPLRRYPGPKLAAASQLLNVYHVLKGDNCKWTAQLHEKYGTVVR

IGPNELSYISPSANQTIFGGRPKEDKVFEKNPVAYLQGNGDISNIFFARFHDHNRLRKLMAPAFSETAVREQEATIQGYT

NQLIAALRNRSGQAAYPDAKGVVNIIPWLHFILFDVLTRLSFGDPIGCLDRADYHPWVSVIFKAIIHSTYTQAAHRLAPY

QWILKHFIPNDMTANYEAHLEFTRKQLDQRQQVKEEPVARADFSSFMLKGMSPDELFDNVNIVITAGGETTASTISSSLY

YLVHNPSSYERLTKEIRDTFSAEGEITLAAVAALPYLKAVIQEAMRIHPPVPIGLFRVAPAAGAFIDGQWVPGNTWVSVA

NLAASRSPTYWRDPERFTPERWLGDAKYESDVREASAPFSIGTRNCIGLNLANANMRIILARLLWNFDFEAQPDNIDPHE

LDEYGIWETKPLNLKIKERVQTT*

 

>CYP65AY2 Penicillium chrysogenum Wisconsin 54-1255

AM920421.1

61% to CYP65AY1

        MQLLEWVVALTGLVWHTQGSFTPSLEQSTNLVQ

364847  WIISHLCLAIYNVFFHPLRNYPGPKLDAATGLVYVYHMVRGNSCKYLAGLHEKYGEVVRA  365026

365027  GPNEISYMTVSANKTIFGNKTTEKMSFEKNPAVYIQGEGTAQNILFASTGEHPRFKKLMS  365206

365207  PAFSEQAIKEQEPTIQQYTSIMIDALRNNRSGEACYPDANGIANIGAWCNFLIFDILSCL  365386

365387  SFGKPIGCLQMADYHEWANVIFGAMKHSHFLQCAHRLKPYHRLLEKLIPGDISGPYETHM  365566

365567  QNVRKNLRERETMENLARADFASFIPKGMSEDELVDNVNILATAGSETTATTLSSLFY  365740

365741  YLTHNPDSYQKLVDEVRSTFSSEEEITFNAVASLKYLKAVIQETFRIHPSVPVGLHRITP  365920

365921  KAGSYIDGKWVPG  365959

365982  QTWVSVALLAAYRSPRYWKQPEDFLPERWLEDPEFASDNRQIWAPFSIGPRKCIGIN (2)  366182

        LAYLNMRLIVARLLWNFDFDPQPDNVDPHELKEYGVWQG  366356

        QVPLNLKIRDARA*  366398

 

>CYP65AZ1  Aspergillus oryzae

           GenEMBL BAE63025.1

           39% to 65S2, 41% to 65Z2

           formerly CYP5118A1

MFVLPTTFMSVSHGIYQLLFLALLATAWRIYSNRFRHPLSHYPGPFFWTISRLPYAVTYAQGTLHRRVRQ

LHDVYGDVVRVAPDELSYRTEQAWKDIHGYSRNFPKDMRFYQTSKNKAPSVVIAPDGVHGRQKRSILRAF

SERAMKSHEHLLRPFVDSLIQKLQHASTSTEGGAVDLTEWYNYIMFDFMAHELFGQSLGCLENGVNHPWV

DMLFGSIKVWAFLSQSKYFPNLSWIIKTAVRLFCRDLLNHRSKKLGCIASKIPEGEGSDSSLPTFNSFLR

ANKGPHSTLSTEEVLSNHSFMMMAGSETTATLLSGCTFFLLKHPQVYKKLTSEIRNRFSSSTEMTFSSLA

NMAYLRAVLQEALRMYPPLPLGMPRVVPPGGAIISGQFVPEKTSVAVASWATYQSSSNFNTPQMFLPERW

LDTGPGDNDVKGAMQPFSVGPRACPGKNLAFGEASLILARLIWEFDLELSPQCSSWAYQRAYIIWDKGPL

LIKLTPRI

 

>CYP65BA1 Aspergillus terreus

43% to CYP65AF1

ATEG_07093.1 fusion gene revised

MTILADF (0)

VYLLASVGYDILFHPLANYPGPKLRAASLTPYLWALISGRHHTSVSDLHRQYGPVVRIAPNKLSYITPQAWKDIY

GHKKANEPEMAKDAKWQVADPNSPSTREQHGHYRRLFSNGFSNRSLREQEPLLQGYIDMLMDGLERAARDKHPVDIVQWF

NWTIFDIIGDLTFGESFECLEKATLHPWVGNMFQAIKGNMLIRVMQETPGLGPYTAGILRYVLPGKALKKREAHFQYTRE

KVNRRLGNLSPRSDFMDHVLHQPEGRGLTSPELLSNSSLLIMAGSETTATLLSGAVYLLLTNPGKMQKLVDELSTVFPGG

SMVNIKSTSRLTYLPAVIEETPALPAACCWDSSRCTGWRLGYRWTTSVAVHHLAAYHSPLNFEQPEDFIPERFIDTSAFQ

DQRDVLQPFSVGPRNCIGKNLAYAETRLILARLFSQFELELMSESDGWIDQRTYVIWEKPPMVGLTRRQGA*

 

>CYP65BB1 Mycosphaerella fijiensis

46% to CYP65AF1 Aspergillus oryzae

45% to CYP563A2 Botrytis cinerea

44% to CYP65S2  Nectria haematococca

43% to CYP65Z2  Neosartorya fischeri

e_gw1.18.265.1 revised at N-term

MYSISTAAPLLALLV (0)

AVSSIVWKIVYNLYFHPLAKFPGPRLNAASSLPGIFALLKGRLPLENKKLHEKYGSVVRVSPNELCFNSVQAWEDIYGHRP

GHTNMHKDPIHVGSVEAVQGVSTLTMSDDENHARQRRALAHSFSQKALLEQEYIVKRYVDQLIVNMKRMADDDEAFNLVN

WLNFTTFDIIGDLAFGEPFGCLDLGRFHEWVALIYETVKAGAIEQATRRFAAAGSLTQRILFNMIPARVRGYRSEHLRRS

RAKCLQRLENGNSQHKDFIWYILKQREKHDLKQDEIIVNSALFIVAGSETTANLLSGLFARLIWNPDKYQKLVDEIRSSF

KSEDEIVYEKLSKLAYLNACIEEGLRIHPPVPTGLLRTVPKEGDTIDGQWIPGGTSVAVSSWAASHNPVNFRDCDSFIPE

RWLDKAYDTDYKKAAQPFSLGPRGCIGRHLSYMEMRLILGRLLWNFDVVSTDGAWQWNPEGEMKNMRAFMTWEKVSIIQY

YMQNSVLTRRSLT*

 

>CYP65-un1 Magnaporthe grisea MG09636.4  poor match 39% to MG02982.4

AACU01001669 cont2.1850 PSEUDOGENE

MANVSELQLSSNKSQFKSGSLKARQFCVDV

6111 IYNLCFHPLRKIPGSKLHGAT 6052

DELETION OF 143 AA

6020 DLMSQLVFGNSLGLLDGNPQYVGWVRAMLSGLKWVVL

TAVLMQVPLLGSLLQALATGPMKGIVGAHYRLASELVDRRLDPKSAHTGK

PDIWSFVIGRQPHETRKQTKDLTRDEAHVAASMLMIVGSETTATTLCGLM

YYLGRQANRRVHDRLAREIRSTIRNSDEIQNDPGMLAQMPYLNAVIQEGV

RLYMPGGITPRVVPGAGATICGEFIPGGTRVAYHSPAAFTNPMNFTRPHE

FLPERWLNPDDSEFAGDRRSVLQPFSVGPRSCTGKDLAWAELRMFLAKVI

WHFDVALCAESENWIVGQKTYISYQKDPMRCKFTPATAGSSGISSASS*

 

>CYP65-un2 Aspergillus flavus

45% to CYP65L1

AFL2G_11416

SSITTSSGLFRAIRSNCENIGSAGRIMVTELRNRVSFNSVTVNQCKYLVAVLQET

MRLWPAIAISLPRVTPPEGCEIDGSWVPGGTKVGVSQWSAYRSERNFARADQFLPERWLPEGEEESFINDTRAAFQPFST

GPRNCLGMNFARAETRIIFARLLLDFDLELLTGRDEWEAQKVYIIWDRCPLYVRVRQAKRR

 

>CYP65-un2 Aspergillus oryzae

45% to CYP65L1

100% to CYP65-un2 Aspergillus flavus

Supercontig 26: 405493-406148 (-) strand

SSITTSSGLFRAIRSNCENIGSAGRIMVTELRNRVSFNSVTVNQCKYLVAVLQET

MRLWPAIAISLPRVTPPEGCEIDGSWVPGGTKVGVSQWSAYRSERNFARADQFLPERWLPEGEEESFINDTRAAFQPFST

GPRNCLGMNFARAETRIIFARLLLDFDLELLTGRDEWEAQKVYIIWDRCPLYVRVRQAKRR

 

>CYP65-un3 Aspergillus terreus

36% to CYP65AQ1

one frameshift, possible CYP65 pseudogene

30/31 top hits are CYP65

ATEG_04028.1

MATAIISSLAFCLIILSISHKVVYNLYFHPLARFPGPSLWCASRIPYLWKLCRGKLPGKIRAYHDKYGPVVRIAPDELSF

DDPAAWREIYLDQTLEQPSRRRTKPTGLFMENLI

VAHPETQPRMRDALDLAFSEAS &

VDKYEPIVTRAIDRLIAKLKSSITKKNDGASALNIARCLELA

SFDIAAEVIWGSSFECLEGGQGNVVVDYALS

RKERLIAMALTFYPFLAFLSPLLSLSSTRTTTLQLFRRKTTERISKHQATSSGQQSQPDVLGSILQSRASETLPVTALEQ

TPVELVMDGGGPIAAVLIGTLNNLLRNPSKYDYLVQEVRGSFKRETDINGSATRPLPYLHAVVTEGLRIAPPFPDGVRRV

VPAGGITVAGCPVPPKTVVSTGCWVQFMNPRNFACPEEFVPERWLHAKGAGAIGGRTIVDTANKDAFYPFGLGARQCLGE

PLAWLELKLTLARLLWNFDISIPPEKTLPVWGEQDVFWTWNVQPMEVSLRLCRRF*

 

>CYP65-un4 Mycosphaerella fijiensis

46% to CYP65-un2 Aspergillus flavus

e_gw1.3.1109.1

LRLYLPVPVVHPRRTPRGCCVIDGWWVAGDTQVSINQYAAHHDEEAFHRVHEYIPERRLPDRPDEFRNDRLGSVQPFHIGPRNCLG &

MQNLAWHETRRLLAAVVLDFDVELAMRRKQW

 

>CYP65-un5 Mycosphaerella fijiensis

45% to CYP65AS1 Mycosphaerella graminicola

e_gw1.8.672.1

VVYLVTVAIYNIFFYPLARFPRPRLRAVSELLYFLCLVRGDTPKKTLDLYNRYSPIVRVSPNELSFISPVAIKEIYGSILG

RPELRKDNKYYSGIGEATLLFSDKEYYS *LRKRLVYSFL

ESALRL*EPII*QLVDVFIIYLKKES*A*RQIDLVK*YNFFAFDIISYLTYSETLDCLN

TSTLYI*V*KFRNLSRPIAFS*ALEKLLKLLRYPFIATFLTKKFISDYKTVYAVS

L*EKVSYSLNRISDTTGFLDKLIATYKEG

KITIF*LNSNATFLIATGTETLVALLVYTTY*LVIYPNKL*KATEEVRSKFADS*EMNIA

AIN*Y*YLLVCLDKTLRI*ALSLVTYPRFTPKDRLLIDS*FIPGNVAVRVLIFAAYRSKL

NFRNPNKFIPKR*T*EDPKYGNDRREAT*VFLVGLRDCLSRNLAYIEIRLILARLI*HFD

IVRLFPED*AE*KVYLV*EKPILIVKLKLVRNSIT*

 

>CYP66A1 Z82021 Agaricus bisporus (a mushroom)

WSYINSIVANSLWNSVKERMEAGTAKPCIATAMLEDLLDDDSAE

SKEEETVRRGACANGFLGGADTTVSLVTSFFMAMALYPDVQKKAQAELDQVLGGRLPE

FSDRPSLPYVNALLKESERWQPVFPLAIAHMSSNADEYDGYYIPKGTYVIGNAWSILH

DPEFYKDPLVFNPDRFLKDGEIDPSVRDPNVASFGFGRRICPGRFFPDASLYSTVTHV

LTVFDIKPNLDENGKEIGIKPDMTDGLLSIPM

 

>CYP66B1 Agaricus bisporus partial mRNA for cytochrome P450 (cyp gene).

AJ271707

50% to CYP66A1

QLKSVRTFIRNVMESPDEFSEWIHFYTSSSIMEIIYGMKAKPED

PYVDNAKKAIEGFNEAAVPGKFLVETFPVMKHIPSWFPGAGWKRQALFWRDVNREVRV

RPFNLVKDQVNEGTATRSVCRTLIGNLPDSTAPDRIVKENIAIDTCAVSFIGAAETSH

SAARVFFMAMLMNPEVQKKGQAELDKVLNGRLPEPNDGPNLPYISAMVKETLRWQLVL

PLAVPHVAIEADEYNGYYIPKGTIVFGNSWTFMHDPEVYKDPESYMPERFLKDGKLDS

SIRDPSTAVFGYGRRICPGRYFALNALYLMIAHTLAVFDIKPALDENDNEKEFKADVT

GGMISQPVPFQCMIVPRSKAAADLIQNSDLME

 

>CYP67A1 Uromyces fabae U81739

FIVLISATAHFIFRRREPTAFQYACFQAGLALLLSVLLREPFGA

VVLILLALNFLLGVQIALYRLFWHDLRVFPGPRLAAITQGWILREAYLGRSRFSMKEV

GETYGDWVRIGPNELCTTSIEALSTIMGPKGWPKGPSYDSGITKGDSGGDSVLTIKNL

PEHATRRRIWNKAFTPNAINGYLPSIEIRLEEMLSVIDTEIKKGESVDLCLQLGCFVY

DTMCDMAFG ALAGSAFSKTQEDKYRILTHMGRVVRQVGIVRNMPWLTPIVKAWPSSHR

REQNEFKEFTKSMFLRRKNQGLGKQLDVFHYL

LGEDTETGTRLTEAELAADSTLLVI

TGADTTRTVLLAFFLYLLKHPNYMEQLQAELLAAPDLSPPSLSRLEYLNACLQETMRL

QPPSPANLQRICPPGGAVICGRQIPEGTKVRFSNYAIHRDERYFSRAEEFRPQRWLQKA

KDDLGNQGEEKERLDQRAFFGFLIGPGACVAKNLAWMEMRLVVATILTNFDLSFAPGF

DPVAFESSWTDAYLLLIEEPFEVMFTPKSQRMR

 

>CYP67A2 Puccinia graminis f. sp. tritici

76% to CYP67A1   Uromyces fabae

PGTG_03841 revised

MNYPASMNEFTSATFFSFLVIFPT (0

VAHLIFRNHEPTAIHYLCSQAVLFLLLEIGFQNSFFTGLLTLSAFNLSLAAQIAFYRLFWHDLR

SFPGPKLAALTQAWILREAYLGRTRFTMKELGEKYGEWVRI (1)

GPNELYTTSIEALYTIMGAKGWPKGPSYDSGITKGDLGGDSVLTIKS (1)

LPEHATRRRI ()

WNKAFTPKAIAEYLPSIENRLDEMIGVIDDHVKQKRNVDLCLQLGCFVYN (2)

TMCDMAFG (1)

APAGTGLLKNQGEKHRILTHMGRVVRQVGIVRNMPWLTP

LVKAWPSTQRKEQSEFRDFTKSMFLRRRNQGFGSQLDVFHYL (0)

LGEDTETGTRLTAAELAADSTLLVI (2)

TGSDTTRTVFLVFFLYMLKHPSYMEELQAELIAAPDLSPPTLARLPYLNACLQETMRM

QPPSPANLQRMCPPGGAVICGRHIPEGTKVRFSNYAIQRDERYFHDPDEFRPQRWL

RNAEEKLREGEDTERLNEKAFFSFLIGPGACVAKNLAWMEMRL (0)

VVATLLTSYDVAFAKGFDPVAFEASWTDAYLLLIEDPFEVTFTPKAGRLR

 

>CYP68A1 Gibberella fujikuroi P450I gene Y15277

MANHSSSYYHEFYKDHSHTVLTLMSEKPVILPSLILGTCAVLLC

IQWLKPQPLIMVNGRKFGELSNVRAKRDFTFGARQLLEKGLKMSPDKPFRIMGDVGEL

HILPPKYAYEVRNNEKLSFTMAAFKWFYAHLPGFEGFREGTNESHIMKLVARHQLTHQ

LTLVTGAVSEECALVLKDVYTDSPEWHDITAKDANMKLMARITSRVFLGKEMCRNPQW

LRITSTYAVIAFRAVEELRLWPSWLRPVVQWFMPHCTQSRALVQEARDLINPLLERRR

EEKAEAERTGEKVTYNDAVEWLDDLAREKGVGYDPACAQLSLSVAALHSTTDFFTQVM

FDIAQNPELIEPLREEIIAVLGKQGWSKNSLYNLKLMDSVLKESQRLKPIAIASMRRF

TTHNVKLSDGVILPKNKLTLVSAHQHWDPEYYKDPLKFDGYRFFNMRREPGKESKAQL

VSATPDHMGFGYGLHACPGRFFASEEIKIALSHILLKYDFKPVEGSSMEPRKYGLNMN

ANPTAKLSVRRRKEEIAI

 

>CYP68A1    Gibberella proliferatum (teleomorph sexual form)

MTNHSSSYYYEFYKDHSHTFRRSMSENTLISSCLALATCAILLS

IQWLKPQPLIMVNGRKFGELSNVRAKRDFTFGARQLLEKGFKMSPDKPFRIMGDVGEL

HILPPKYAYEVRNNEKLSFTMAAFKWFYAHLPGFEGFREGTNESHIMKLVARHQLTHQ

LTLVTGAVSEECALVLKDVYTDSPEWHDITAKDANMKFMARITFRVFLGKEMCRNPQW

LRITSTYAVIAFRAVEELRLWPSWLRPVVQWFMPHCTQSRALVQEARDLINPLLERRR

EEKAEAERTGEKVTYNDAVEWLDDLAREKGVGYDPACAQLSLSVAALHSTTDFFTQVM

FDIAQNPELIEPLREEIISVLGKQGWSKNSLYNLKLMDSVLKESQRLKPIAIASMRRF

TTHNVELSDGVILPKNKLTLVSAHQHWDPEYYKDPLKFDGYRFFNMRREPGKESKAQL

VSATPDHMGFGYGLHACPGRFFASEEIKIALSHILLKYDFKPVEGSSMEPRKYGLNMN

ANPTAKLSVRRRKEEIAI

 

>CYP68B1 Gibberella fujikuroi P450II gene Y15278

MSIFNMITSYAGSQLLPFYIAIFVFTLVPWAIRFSWLELRKGSV

VPLANPPDSLFGTGKTRRSFVKLSREILAKARSLFPNEPFRLITDWGEVLILPPDFAD

EIRNDPRLSFSKAAMQDNHAGIPGFETVALVGREDQLIQKVARKQLTKHLSAVIEPLS

RESTLAVSLNFGETTEWRAIRLKPAILDIIARISSRIYLGDQLCRNEAWLKITKTYTT

NFYTASTNLRMFPRSIRPLAHWFLPECRKLRQERKDAIGIITPLIERRRELRRAAIAA

GQPLPVFHDAIDWSEQEAEAAGTGASFDPVIFQLTLSLLAIHTTYDLLQQTMIDLGRH

PEYIEPLRQEVVQLLREEGWKKTTLFKMKLLDSAIKESQRMKPGSIVTMRRYVTEDIT

LSSGLTLKKGTRLNVDNRRLDDPKIYDNPEVYNPYRFYDMRSEAGKDHGAQLVSTGSN

HMGFGHGQHSCPGRFFAANEIKVALCHILVKYDWKLCPDTETKPDTRGMIAKSSPVTD

ILIKRRESVELDLEAI

 

>CYP68C1 AY040587 Fusarium sporotrichioides TRI15 gene

MSKVDKTGYAQWVYLLPERSSMMSFIDSMEDFRFDMLIYFLCFV

VLGRAVQWFLRPKPNAPLLNPRRFFEFSDSRAVSEILYSTRQVLEDWFSKYPTKPMRI

IADLGQITILPPSMADEIKNDPRLSFIKASTESAFHITIPGFEPFREGAKNEAGLIKN

VLHKHLNKTLNHITTPLAEETCLAVQEYFGSDQGWHKVPLRDTLVPLVTRISTRIFLG

QDLCRNQEWLRIAASYSSTSAEVANHLRRWPKPLRYLVSLLSPECQNLAKQVRNARAL

INPILERRRVEEGQEKGTSYNDSLEWFERYAREAYDPAATQLFLSVVSIHTTTDLLCQ

ALEDISSHPEIIKPLQHEIREVLKQEGWNTKALYKMKLLDSVLKESQRLKPVQHATML

RLALEDITLEDGTFIPKGHQISVSCHAMRDNEIYENASSWDGYRYYRQREQSANEHKA

QLSSTSPEHMGFGYGIHVCPGRFFAANEVKVIMIYLLLQYEWRTPPGSQPKPLSWCTT

WATDPTFELEVRRKGSDDIPVELSHNTFSRESES

 

>CYP68C2 Fusarium graminearum FG00071.1 AACM01000004 FGcontig1.4_scaffold1

MALITSLQDVRLDMLAYFVAFLVVVSVVRKKLAPQPSAYLLNPRRWYEFT

DARAVSEVLHTTRQTLEEWFHKHPTTPVRLTTDFGEMTFLPPTLADEIKS

DKRLSFIKAANDSAFHTEIPGFEPFREGGRNEAALIKEVIHGQLKKTLNK

MTFPLAQETQLAVEHYLGANKEWHKIRLRDALLPLVTRISTRIFLGEDLC

QNDKWISITSEYAANSLEVANRLRVWPKYMRYVVSYFSPGCGILRNQVKN

ARELITPIVERRRSEEKGKEYNDSLGWFEKTAKQAYNPAATQLFLSAVSV

HTTTDLICQCLEDIAAHPEIIKPLQEEIRRVIAEEGWNTKAMYKMFLLDS

VFKETQRLKPIQVASMVREAQSDITLSDGTFIPKGHQIAVSCHNMRDGRI

YENPEKWDGYRFFRERQQSAREDKVQLSSTSVEHMGFGYGEHACPGRFFA

AKQVKIVMMYLLLNYEWKIPEGPEPQLMAWCTTWVTDPDYEVLMRRKDKD

DPCLRLELVQDD*

 

>CYP68D1 N. crassa AABX01000146.1 cont3.306 NCU05376.1 (version3)

10526 MANPSATPSSIPSWMERLDIKSITDPSATPFSYLVTAFLLAVVV

      YSLQGPRFPKNIKHLNPKGPLEFSDTRPKKEFVYGSRQMLANWF

      KANPNKPCRVISDFGEAIVLPPRMANEIKNDDRLSFTRWTYK (0) 10915

10981 AFHGHLPGFEGFGEASRESHIVQEVIMRDLTKYL (1) 11082

11140 NKVTEPLAQETSMAMEANLPKAANGEWSTINLRSKI

LPIVARISSRVFLGEELCRNEEWLKVTQQYTIDGFGAAEDLRLWPAALRP

IVHWFLPSCQRARADVRVARSILDPVLKKRRQEKAANGGKAEHDDAIEWF

ERTAKGKYYDPAVAQLVLSLVAIHTTSDLTCQVMTNLMQNPEFIAPLREE

MIQVLSEGGWKKTSLYNMKLLDSVIKESQRVKPTGVASMRRYAEKDVTLS

DGTFIPKGGFVAVSAHDMWNSEVYEQAEKWDGRRFLRMRETPGAGKENVA

QLVSTAPEHLGFGHGQHACPGRFFAANEIKIALVHLLLNYEWRLPEGSDP

KIRTFGFSMGVDPSLKVEYKGRQPEIEL* 12234

 

>CYP68D2 Nectria haematococca

e_gw1.16.35.1

Necha1/scaffold_16:246007-247770

55% to 68D1 48% to 68C2

This gene model seems correct

MLVKELLEAPWAFLNSTASASNIRLSYFVAPLIAITVACFFFTSKPNAPCLNPQGFFDIASGRAKKQFLF

GLRSMLKTWFDANPHKPASVFSDIGPMTVLPPSMANEIRSDPRLSFVEFSAK (0)

FFHTSIPGFEAFNEGTRDSITLTVINKDLTKRL (1)

AQVTQPLAEETTLAMQEIFTDNK (1)

EWHLINVREKILHLVARISSRVFLGEELCRDE

AWLKITREHAMNGFVAADLLRAWPEALRPVVSWFMPHCRTARSQIREAEKIIGPVISKRREAKDAALRAG

REAPIHNDAIEWFEQASKGKPYNPALSQLFLSTVAIHTTTDLLCQTMIDIARHPEYFEPLREEVTRVLAQ

DGWKKTSLHSMQLLDSVVKESQRLKPLQL (1)

ASMQRLAVKDVQLSDGTFIPKGTASCVSSHALWDPDVYEAP

DTWDGHRFLRQRGIPGKENFSQLVSTSENHLGFGHGKHACPGRFFAANEIKIALAHLLLEYEWRLPEGEA

LDVEDFGITPIMNQTLKMEFRKRD*

 

>CYP68D3 Aspergillus niger

e_gw1.6.1239.1|Aspni1

60% to CYP68D1

MPVPVLVSLALGLAFLLLQSFRYKDPALNPKKPFELTSTRVKQEYFSNARRLLWDWFTTHSNTPVLLHTDTGKVTILPPS

MANEIRNDDRLSFSRWTFEAFHGNLPGFDGFREGSQDSGIVQAVIGNDLTKYLNKVTEPLANETSVAVRELLTDNEEWHT

IQLRTVMVALISRISSRVFLGEKLCRNEEWLRVTQDYTTDGFLAAEELRMWPAAIRPIVHWFLPRCRKLRAQVKVARKVM

GPILEERRQFKKHLHAKGKQSQGFDDAIEWFEKAAKGRPYDPVSAQLILSVAAIHTTSDLTSQTMTHLVQNPEILEPLRK

EIVDTLQQHGWKKTALYNMKLLDSVIRESQRLKPINNASMRRVALKEVKLSDGTVIPKNGMLAVSAHKLWDDDTYENAAA

WDGYRFYKMREDPERQTKAQLVTTAPENLAFGHGKHACPGRFFAANEVKIALIYLLLRYDWKLLEGTVPKIRYGGFSMAV

DPSLKMEVKRRREEMGI*

 

>CYP68E1 Magnaporthe grisea MG10527.4  with C-term extension = accidental fusion

AACU01001764 cont2.2024

MSTLELFSNITSPVSDDLRDGTAFKYAPYLVTAIFVLAIVYSKRESYPDL

PRLNPKGPLEFGWAKRLNHFMVHSPELLAEASRRFGDNPFKLFTDLGDIV

VVPAKYANELKNHRGMNFMEVGVDSVHTYLPGFEPFAATPALVKVVNNNL

TKTLAKLTAPMVQEAALGSSDIFGESKEWHEVSIEQIMGFILRISSRIFI

GQELCRSKQWLEASRAWTHVAFQAVFTLRAYPRFWRPWIHWSIPCVNDSR

RKLKLCREVLQPYIDKRNIVKAEAIARGEPSPFDDSIEWFEQENKTDFDP

AQKQLQLIVAAVHTTTDLLVVALVNIAKNPEIIGPLREEIISSLSEEGLK

ISAFEKLKLMDAVFKESQRLKPIATIAFRRKAMEDVVLSDGFKIKKGTII

GIDGIKALRDERVYPDPLRWDPYRYIKLREAGEAAKARLVSVTQEHFGFG

HGFHACPGRFFAANELKIALARIILNYDITVPEDVKNLPEAFSGSNYKIP

KGTRFLFKRRKEELDLSTLV*

 

>CYP68F1 Magnaporthe grisea MG00300.4  36% to 68A1 35% to 68D1 AACU01001402 cont2.70

MANETFSAISQPGAFPGLGAQTSAWASYVVAALLLASLAYSRRDKYRGVP

DVTPSRPFELTDSRKRKEFLAKSREILAQGSKRYGDQPYKVTAEMGQILV

LPPQYVAELKNNPALDLTAFVQKILVHAYLPGFEPFGAAEATVKIIPKYV

NKKLGNLALSDIFTDSKDWTEIDAFSILRLASRMSSRIFMGKDICKDPVW

FKALLDYISDIGAQRNIISGWPLSLRPIIYRLVPASFRIKRKLQKCRDVL

KPHLERRAAIIKDALARGEPNPFDDSIQWYENEVGDGEKDHAVFQIGIFL

VAIPNTADTLMQTLHHLALNPEYISVMREEVIRVIGTHGLNKAALQKLDF

MDSCLKESVRLRPVLTLYFQRMALQDVTMSNGFTIKKGTVLGMDGTRMMM

DEDIYPEPERYNPRRFMEMRKLPSGASKAPMVTVTPDHHAFGHGIHSCPG

RFFVTTALKIALAHMVLKYDWKVAEGYEDAKPRVMGMRYLASNVKLQVRR

RKEELDLDSLAAQTK*

 

>CYP68G1 Magnaporthe grisea MG03834.4  40% to 68B1 AACU01001031 cont2.748

MNSPNQSVQPSSAAYAGPEQPLVSSMFERTPLLGLGLSVAVATLFLGKFL

FGGGSSKKLAFPLVKKDGDGYNFLSDALKMVEKGRSQHPGMPFFIEGENG

PLAIIPPNMIDDIRSHEDLSFHLTVREDFHDGMPGFEAFTITSRGGGVLQ

TVISKQLTKYVGVILNNLSDEAAYALHKNFGDDTEWREHTIKPIILDTIS

RVASRIFLGEDLCRNEEWLELSKGYTIAAFMAARDLRQRPFHLRRLMQWF

EPSCKTLRRYRDSSRALILPIVDKRRALRRECIAQGRPLPVFNDCIDWFE

EEAAGRDYDAGMIQQAIAATGNHTSSDFTTKFVLILAEHPEVQQEIREEI

VRVLGAEGLTKAALANLKLLDSAMKETQRLQPLTTSPMARMATKDIHLAN

GVCIPKGTPLVIDGSWRLSPDIYENPLEFDAHRFLKWRGTDRENMAHFVS

TAASHTGFGHGIYACPGRFFATNELKVILCQLILKYDWKLAEGSTAEPLV

FGFNLDASWDAKIMVRRRKVVELDIDALSA*

 

>CYP68H1 Magnaporthe grisea MG02294.4  41% to 68B1 39% to 68D1 AACU01000444 cont2.449

MASSINASPTWPQGQLPGLSALASLDLDRSTIAITLAAIVILPLLVSYIA

SSLRGEKVPVVNPPGRFQTALQKSIEFGTAGFKIFQQAQEQFPNQPFKLL

SNAGYTTVLPPDRALEVKQHPNLSFRKAFSTILPANSEGAAALGVVDHPS

EIIQKIVTKHLTKRLNSVTAPLSLETSVATEKNFGKSSEWTEIKVYEPIT

DVIARVSSRVFLGDKLCRDAAWLDITKSFAQTAMAVIMTMRAFPTWIRPL

VFQFASYGKEYRALYRRGHNAIMPIMKERQEERAECAAKGVPAPVYNDLI

EWAETEAGNNSFDMTAVQLSISMAAIHTTSDLVSQNILLLSTRPDFVDAL

RKEMIEVLPAKGWKKTSLTSLRLLDSAIKEAQRLKPTSVIGMQRLVMKDT

VLEGGITLRKGEYSAVDATRVWDPKLHKNPDEFDIYRFYEARKQPGGEHV

HQLVSTSPDHIAFGLGKYACPGRFFASNETKVVLCHLLLKYDWKLAEGSS

TDFTYFGTELTINPETKVLYRRRKEEFDLDALATDEEAS*

 

>CYP68H2 Magnaporthe grisea MG11075.4  38% to 68B1 AACU01001898 cont2.2223

MASSSKLDLAWLQKQPVGQGFLEMSQSTIAVTLAALVLVPVIASYIFAGR

KGAAEIPLMNPPGPFELALSKAVQFPQQGLDLYNKARKLFPDQPVKFITN

AGTLTILPSDRAQEVKGIRSLDFRKSFSYSAPLTAPGGAALSTFDHPNEI

VQTVVSKYLTKRLRPLASEATFTINKNFGNSPEWTEVKMYDVGLDVVARL

SSRVFLGSEICRNEEWLETTKRITMVFTSVLQTARVYPTWLRPLVYRFGK

HGRDMMAVTKQVNDIVNPIVEKRKLEMAECEARGEPAPVYNDAIEWILSE

HDPNDRIFKVADFQIALSVAAVHTTSDLLSHTMLTLAANPQHIEPLRKEM

IEVLGTGGWKKTSLTSLRLLDSAVKEAQRIKPVQRVSMQRLVTEDTTIEG

GYRLRKGELVGVDTYPLRDPAKWPEPETFDPYRFYQARKQPGGEHKAQLV

SVSPDHLTFGYGKYACPGRFFAANEVKLALCHLLLKYDWKLPEGGSDQPI

MFGLDPIVNPGATIMFRRRKEELDLDSLLVDSEE*

 

>CYP68H3 Magnaporthe grisea MG00024.4 41% to CYP68B1 41% to 68D1 AACU01001352 cont2.7

MASSVHADSGWLRKQVMGSSGLELLDSSRFTIGITLAALVLLPVVASYLT

GARKGKEAPLVNPPGLFQLAVQKNIEFVHKGNYYLKEARRRFPNQPFKLL

TNGGPMTVLPPSRSNEIKNMESLDFRKVLSYAAPVNLPGGSAIAAVDHPQ

AIIQTVITKNLTKRLNTVTGPLADEASFAINKNFGSNPDWVEAKICEPIT

DVVARLSSRVFLGEEICRNEEWVDLTKKIAIVVLTCIYTLRAFPQWMMPI

VYTFGKAGKDMRAMRTRVIEIIHPIIDKREQEKAECKAQGIPAPVYNDAI

EWVAAEADPNDPVFKLGDFQIGLSVAAIHTSSDLLGYTLLWLAASDPAYI

DALRKEIVEVLGKKGWKKTSLVSMKLLDSTIKEAQRLKPVQLAIMQRRAM

KDIESEGGVTVRKGELVAIDSNRLRDPAYYPNPDTFDPYRFYNARKQPGG

EHKAQFVSVTPDHLTFGYGKYACPGRFFAANEIKLALCHLLLKYDWKLPE

KAPPKPLIIGIDPLVDPEARLLFRRRKEEIDLDSLDVENEE*

 

>CYP68J1 Fusarium graminearum FG02672.1 AACM01000132 FGcontig1.132_scaffold1

MICRALDASTVCSYKTQHSSSMATDLDLVLGKSQYALFCGITLFSFFILK

YSLLGNGGKQYPYINPKKPFELSNQRVVQDFIENARDILTKGRSLYKDTP

YKAHTDLGDVLVIPPEFADALKSERQLDFTEVARDDTHGYIPGFEPIGSP

FDLVPLVNKYLTRALAKLTKPLWAEASLGVNHVLGTSTEWHPINPGEDIM

RIVSRMSSRIFMGEELCKDDDWLKVSIEYTVQLFQTADELRNYPRWTRPY

IHWFLPSCQGVRRKLQEARDLLQPHIDRRNAVKKEAIAEGRPSPFDDSIE

WFENEYEGKSDPATEQIKLSLVAIHTTTDLLSETMFNIALQPELLGPLRE

EIVTVLSTEGLKKTSFYNLKLMDSVIKESQRLRPVLLGAFRRMALADVTL

PNGDVIKKGTKIICDTTHQWNPEYYPDASKFNAYRFLQMRQTPGQDKRAH

LVSTSHDQMGFGHGLHACPGRFFAANEIKIALCHMLLKYDWKLPEGVVPK

SKALGMSLLGDREAKLMVKRRAAEIDIDAIGSDE*

 

>CYP68J2 Fusarium graminearum FG04717.1 AACM01000196 FGcontig1.196_scaffold3

MSTYIDIVLENPQYAVLSTISLIAILIANYSFFAKDDKYPVLNPKKPFEW

SNTRVVKEFTENSKSLLAHARSVYGEQPYRAYTDMGKVLVIPPSWVHALR

SKKELDFRIPAQEDSHEYIPGFDGFGFHANMPTVITKYVTKSLAKMTGPI

SEETSLSIRDRLTDSKEWHSINPPKEMIRIVSRVSSRIFMGKELCRDEAW

TKASSDYTVVAFASIALLRVYPRWLRPYIHWFLPYCKEARRLLKEARECL

QPHLDRREVIKQQALAQGQPCPFDDAIEWFNNEYDKHDPATQQISISIVA

YHTTSDLLCETLLNLCQHPELFKPVRDEIITVLRQEGGITKAALYNMKLL

DSVIKESQRLRPILLGAFRRKAMADVTLPNGDVLRKGDRVIGETTHMWGP

ESYDNALEFDPYRYVRMRESGEENKAHLVSTSPEHLGFGHGVHACPGRFL

AANEIKILLCHLLLKYDWKLPEGAEPKPSFASFRIDGDKTTNLLIRRRTE

ELDIDSLSA*

 

>CYP68J3 Fusarium oxysporum

68% to CYP68J1

FOXG_10386

MSSYLDLLLGNPQYAIFCGVTLFTLFVIRYSLLGHVTKFPVLNPKKSLELTSNRATQDFIADSKNILTNGRALYKDQPYR

AYTDWGKVVVIPPKFLDALKSHKQLDFTIPAEDDSHAYIPGFDPFVSDPNISKVVTKYLTKSLTKLTAPLSEEASLAFRQ

VLTDSTEWHEMHPQHDIVRIVSRMSSRVFMGESLCRDDEWVRASGDYTVQSFKTGDILRTDPRCSRPFVHWLLPSCWTLR

KKLAEAIQCLKPHLERRSCISAEAEAQGKPSPFDDSIEWYKKEGSTRNPALLQISLSLVAIHTTSDLLMETLFNIAQHPD

LFQPLREEITDVLTTEGLKKTALYNLKLMDSVIKESQRLRPVLLGSFRRQAMADITLPNGDVIKKGEKIVCDTTHMWNGD

YYEEAAKFDGYRFLRMREASEQDKHPHLVSTSFDHLGFGHGNHACPGRFFAANEIKIALCHMLLKYDWKLADGVVPKPSG

FGMAYLPDLQAKLLIRRRTEELDIDSIETC*

 

>CYP68J3 Fusarium verticillioides

95% to CYP68J3 Fusarium oxysporum = ortholog, 60% to CYP68J4

FVEG_09038

MASYLDLLLGNPQYAVFCGVTLFTLFVVRYSLLGHLTKFPVLNPKRSLELTSNRVTQ

DFIIDSKNILTNGRALYKDQPYRAYTDWGKVVVIPPQFLDALK

SHKQLDFTIPAEDDSHAYVPGFEPFVSDPNISKVVTKYLTKSLTKLTAPLSNEASLA

FRQVLTDSTEWHEMHPQHDIVRIVSRMSSRVFMGESLCRDDEW

VRASGDYTVQSFKTGDILRTYPRWSRPFVHWFLPSCWTLRKKLDEAIRCLKPHLERR

SVISAEAEAQGKPSPFDDSIEWYKKEGSTRNPALLQISLSLVA

IHTTSDLLMETLFNIAQHPELFQPLREEITNVLSAEGLKKTALYNLKLMDSVIKESQR

LRPVLLGSFRRQAMADITLPNGDLIKKGEKIVCDTTHMWNGD

YYEEAAKFDGYRFLRMREASEQDKHPHLVSTSFDHLGFGHGNHACPGRFFAANEIK

IALCHMLLKYDWKLADGVVPKPSGFGMMYLPDLQAKLLIRRRNE

ELDIDSIETQ*

 

>CYP68J4 Fusarium oxysporum

70% to CYP68J2 Fusarium graminearum

FOXG_16939

MTAYINTILQNPQYAVLSAISVITLLVVNFSFFTQQEKYPILIPKKPFELTNTRVVKEFIANSRSLLANARTVYKDQPYRAYTELGKVLVIPPSWVDALKSNRHLDFQTPARD (0)

DSHEYIPGFQPFGGSHKLTTVINKYITKAL (1)

MVKLTGPISEEASLSIRDTLTNSR (1)

EWHPIRPQADLIRVVSRVSS

RIFMGEELCRDEEWNRTSSEYTLLAFGYGGLLRSYPRWLRSYIHWFLPQCWEVRAKLNEARQCLKPHIERRKVIKQKALAEGK

PSPFDDSIEWFEKEYEKHDPATEQIAVSIVAYHTTSDLLAETLLNLCQHPELFQELREEIIAVLTAEGGLTKGALYNLKL

MDSVVKESQRLRPILLGAFRRIATADVTLPNGDILKKGDKIIGGMSHMWDSDTYDNALEFDPYRFVKMRQTGDDKKAHLV

STSADHLGFGHGFHACPGRFFAANEIKILLCHMLLKYDWKLPEGCKPQPSHSGFKLLGDYSSNLLVRRRTEELDIDSLSS

SKGHDSLPY*

 

>CYP68J4 Fusarium verticillioides

83% to CYP68J4 Fusarium oxysporum probable ortholog

FVEG_13755

MSAYIDTILQNPQYAVLSAISLITILVVNFSFLTKDEKYPFLIPKKPLELSDRR

VVKEFLANSKSLLANSRKVYKDQPYRAYTEIGKLLVIPPSWVEALK

SNRQLDFLMPTKDDAHQYIPGFDPFGFDPKMPTVINKYITKALTRVTGPVS

EESALAIRDCLTDSKEWHGVKPQEDLIRIVSRVSSRIFMGEELCRDEEW

TRVSSEYTLMSFGYGHILRTYPRWLRPYIHWFLPQCWAIRAKVNEARQC

LKPHIDRRNAIKQKALAEGKPCPFDDSLEWFEREYEKHDPAKEQIAVSIVA

YHTTSDLLAETLLNLCQHPELFQELREEIITVLTAEGGLTKGAMYNLKLMD

SVVKESQRLRPILLGAFRRTATADVTLPNGDMLKKGEKIIGNMSHMWDS

DTYDNALTFDPYRFVKMRQTGDDRKAHLVSTSTEHLGFGHGIHACPGRFF

AANEIKILLCHLLLKYDWKLPEGCKPQPYFSGFKLLGDFSSDILVRRRTEELDIDSLSQS*

 

>CYP68K1 Fusarium graminearum FG11002.1 AACM01000457 FGcontig1.457_scaffold8

MIDEIKNDRRFDFGAAASDDNHAYIPGFGALMHDPVMPKVIGRHLTKALV

LPAALGASANQLLQEWHILNVNEDISTIVSRMSSRVFMGEDLCRDQGWNN

ASAYYTRKTFKAMMILCVIPRWLRPYIHWVLPQCREVRRALAAARKELNP

HIERRERTKAEALARGEEPPFDDAIEWFAQSGSELPPADCQIALTLAAIH

TTTDLLSQTMVNIATYPEIFTALREEITRVLSEQGLKKTALADLKLMDSF

LKETQRMKPILIGWRRRALADVTLSNGMTVKKGNKVVVTAIHMWSDGNYN

NAKTFDPCRFVPKDKEQMSYLVNTSANHLGFGHGTHACPGRWFASNEVKI

ALCHLLLKYDWKLVDGKVPEPVAFGMAYVANPEAKLLIRRRKEELDLSML

EY*

 

>CYP68L1 Aspergillus nidulans AN8530.1 45% to 68A1 54 clan

MDSLSLSNSQSIAGVLALLLLSGIYVYLNSPSYPVVNGKRPWELRIIHAQ

KRFLSNARDLIDSGLARWPAFHLVTEAGYRLVLDPKYANEIRSHEALSFG

KATAQDFHAGIHGFEPFEQGTRSDQIVSDVVRMKLTQSLGNVTKPLSDET

AIALQKNWTDEADWHTIPVKKTVLDIVAQLSSKVFLGDQICRNPEWLRIT

VAYTVDSFLAAQALRMWPTFMRRLVAPFIPGVQKIRAELEEARRIILPVL

EKRKAEKQTAIAAGKTPARYNDAMEWMEQCAKGRPYDAAVSQLSLSLGAI

HTTSDMLTQVLYDICGHSDLVDELRQEVLTVIAAEGWQKTTLYKLKLMDS

VLKESQRVKPIGIVKLSDGTVIPKNANLIVSSQRMWDESIYPSPDKFDPY

RFLRLRETPGHETSAQFVSPSPDHMGFGFGKHSCPGRFFAANEIKIALCH

ILLKYDFRLTEEWRNPRPIASGAGLTAEPRATMEIRRRKEEIQL*

 

>CYP68L2 BAE66422.1 [Aspergillus oryzae]

MLGDLFESVLTNNSVTLLITVAVAAIALHLSSPKSNLPLVNDKKPWEFRFTKARKRFLANAHNLIKAGLA

KAPAFRIVTGNGKRVVLDAKYANELRSHDDLSFGLHIADNFHAHIRGFQPFKQGSNDDEIYQNAVRMKLT

QSLGNLTQPLVDETLVALQTYWTNDTNWHAIPLKSNIQKVVAQLSSRIFLGDQICRNPNWLRIAVDYTVD

AFKAAEELRLWPKAFRAIVALFLPSCRKIRAELQEAQDIIRPVLDARRKDKQAALSAGKEPERYNDAMQW

LEECAKGRSYEPAFGQLTFSVAAIHTTSDMLTQVLYDLCGRDALIQALREEVITVVQEEGWTKPTLYKLK

LMDSVLKESQRLKPISVVSMQRVATADLRLSDGTFIPKGTSLAVSSDRMWDSEIYPNPLEFDGYRFLKLR

ELPGHETSAQVVSPSPEHMGFGFGRHACSGRFFAINEVKIALCHILLKYEFKLADGSVPRATKFGFSFTS

DPTTKLMIRRRQEETVL

 

>CYP68L2 Aspergillus flavus

97% to CYP68L2 Aspergillus oryzae

AFL2G_11735

MLGDLFESVLTNNSVTLLITVAVAAIALHLSSPKSNLPLVNDKKPWEFRYTKARKRFLANAHNLIKAGLAKAPAFRIVTG

NGKRVVLDAKYANELRSHDDLSFGLHTANYFHAHISGFQPFKQGSNDDEIYQNAVRMKLTQSLGNLTQPLVDETLVALQT

YWTDDTNWHAIPLKSNIQKVVAQLSSRIFLGDQICRNPNWLRITVDYTVDAFKAAEELRLWPKAFRAIVALFLPSCRKIR

AELQEAQDIIRPVLDARRKDKQAALSAGKEPERYNDAMQWLEECAKGRSYEPAFGQLTFSVAAIHTTSDMLTQVLYDLCG

RDALIQALREEVITVVQEEGWTKPTLYKLKLMDSVLKEIQRLKPISVVSMQRVATADLRLSDGTFIPKGTFLAVSSDRMW

DSEIYPNPLEFDGYRFLKLRELPGHETSAQVVSPSPEHMGFGFGRHACSGRFFAINEVKIALCHSLLKYEFKLADGSVPR

ATKFGFSFKSDPTTKLMIRRRQEETVL*

 

>CYP68L3 Histoplasma capsulatum G217B

ABBT01000111.1 Ajellomyces capsulatus G217B 382612-384420 (-) strand

57% to CYP68L2

HCB03354.1 revised

384420 MLLETMLNSSMPSTLGLAAIFILLPALFFQVTLPKKSRLPLINGKKLSEFSYTHSGKRFLK (0)

VNARILVDEGLGK

384118 ARAFRLMTDNGPKTVLSAGYADEIRSHPLLNFGAAIAKEFHADIRGFDTFKQGTRADEIFQDAVRMQLTH

HLGNVIEPISTEASIILERDWTNNHGWHDIVLKKSVLKIVAQLSSRVFLGEKLCRNPDWLRITISYTVNSYIAAQALRLW

PEIIRPLVANFLPSCRKLRREIEEARAIINPVLEERRQAKEGAIRVGKTPERYLDAMQWLEESARGRYYDPVVAQLSFSH

AAIHTTSDLLTQVLFNLCGRDELTQKLRDEIVTVTQAEGLKKTTLFKLKLMDSVLKETQRLKPVAVATMRRLAEEDIELS

DKTFLPKGELIVVACDKMWDPDTYPNPSTFDPYRFVKLRERPGHEMSAQLVSTSPEHLGFGFGKHACPGRFFAANEIKIV

LCHILLKYDIKLADGCEPRIRSNGLSLNADPIARISIRRRAD* 382612

 

>CYP68L4P Aspergillus clavatus

ACLA_047580

AAKD03000015.1 117585-116726 (-) strand mid region is 63% to 68L2

MLLDIKTQFSRLVPFSIAGVLALSALFIFLTLPRKSLLPMVNRKGPWEIRSYRVQNKCVQDARKSIKTGLSK

 

EAMQWMEECAKGRPYDAAVAQISFSLAAIHTTSDMLTQVLLDLSEHEQLIQELREEIVTVIRSEEWKKTTLYRLKLMDSVLK

KSQRLKSIGVATMRRLAEEDIKLSDGKLVPKGTSLLVFNETMWDPSVYPNPETF

 

ILLKYDIKLAKGCKPTMRMNGLSMNADYFAKVSVKRRQEETLL*

 

>CYP68L5P  Aspergillus fumigatus Af293

           XP_752004.1 also EAL89966.1

           Internal fragment, needs revision

           Pseudogene fragment mid region aa 183-260

           62% to CYP68L3 Histoplasma capsulatum

           74% to CYP65L5P Neosartorya fischeri

           formerly CYP5088A1

KICRNPDWLRITVGYTVDTF &

LPAENLQLWPRLIRPVVAKF &

LPPCRKICWELKEATEIITPVLEERQ

 

>CYP65L5P Neosartorya fischeri

62% to CYP68L3

Supercontig 569: 1883929-1885384 (+) strand

MAASRIATCMKDKMLTDISTQLFRLDP

ISLAGVLAISGLFFLLTLPSKA &

SIPMVNVKKLFQIRSSHIQKRYLDACKLSKTGSRK ()

APSFRVHSDNGIKTMLAPEYANEFHAHPALRFGRAITLEFHSD &

FLGD KICRNPDWLLITVDYTVDTFFAAEDLRLWPRLIRPIVANFLPSCRKIRRELKEA

PVLEERR DLEAARKANQPQKRYHDAIQLMEECAKGQHYPPAEAQMAFSIVAIHTTSDMLT

QVLLDLCANPEVIQPLREEIVAVNQEE*WKKTALYKLKLLDS

VLKESQRMKPVNI

ASMRRLALEDIKLSDGTLIPKGSSILVSSRKMWDPDAYPNPELFNPYRFLRLRETPGHET

SAQLVWPSPDLGFGYGKHACPGRFFTANEVKIALCHIILKYNFNLPDGYTPTVRKRGIS

LSADPSAKLVIRRRQEEIAL*

 

>CYP68L6 Aspergillus terreus

63% to CYP68L3

ATEG_03869.1

MLDTVTELPRWDLISLIGLSVISTLFFLLSPFQRSPFPLINGKGTLELSSSNAKKRFLADAGNLIKIGLSKASVIRLVSD

NDVKTVLHPKYVNDIRSHPALSFGTAIQKEFHADAGIHGFEPFKQGSTAGEIFQYAVRTKLTQSL (1)

GNVTEPLSSETALTLDTSWTSNT (1)

EWHEVDLRASILGIV

AQLSSRVFLGDQICRNPDWLRITVNYTVDSFIAAQDLRLWPKPLRPLVANFLPSCRKIRSELREATEIITPVLEERRKSK

ETQVREGRTPERYVDAMQWMEESAEGRPYDPAVAQLSFSLAAIHTTSDMLTQALLDLCGKDDLIKELREEIVTVIQGEGW

KKTTLYKLKLMDSVLKESQRLKPVSIASMRRLASEDVKLSDGTIIPKGSSLFVSSDNMWDPSVYPDPEKFDPYRFLRLRE

IPGHETSAQLVSPSPEHMGFGFGKHACPGRFFAVNEIKIALCHILLKYDIRLPEGYTPAKRRMGISLDVDPLARLLVKRR

QEEILL

 

>CYP68L7 Aspergillus terreus

54% to CYP68L6

ATEG_10083.1

MLEPVSTAQSLWSFGLWILVILSPVLFFASRELGILNAKKRFAKNGFEEVLAGLRKSDVFGLMTINGPKIVLAPKFAQEI

RSNPALSVSAFSSSELHAHIRGFDVFRQGEADDILQDTVRSKITQSIGDLIQPLSEECSLTLKPKWTDSPEWHEVCPHTT

ILDIIARLSSRAFIGDELCRNPKWLRLTVDFTVDSFRAAEALNWWPYALRPLVARFLPSCLKLHKYIQDADNMMKPVLES

RRQAQAKDPQKSYPDTIQWFEETAQGRPYDPVRLQLTLAFASIHTTADLVIQTILDLCSAKNWDELCRSLREEIISSFRE

EGWRKPLLAKLKIMDSALKESQRLKPVSIVGMGRIAKEAVKLSNNTIIPKGTRLLVSNTAMWDPEIYPDPRTYDPYRFLR

LREASENESAGQLVSLSPTHLSFGLGKHACPGRFFAAAEVKIILCHILLKYDIKLADGCKPKPLRAGTNLVADPTAKLLV

RRRQEEVAL*

 

>CYP68M1X  Aspergillus nidulans

          Name revised to CYP5073A1

 

>CYP68N1 Nectria haematococca

e_gw1.41.77.1

Necha1/scaffold_41:90805-92550

52% to 68D1 N. crassa, 48% to e_gw1.16.35.1, 48% to 68A1

This gene model seems correct

MLWAELLPRLDAIGKPLPLIVTIAVILFVYHAFQPFRSNVPILNGRKGLELTNQRPKKEYVSQATKLVGD

WFNSNPNKPVRLISDVEEITVLPPSLAQEIRSDKRLSFSQWTFK (0)

SFHGNLPGFDGFAAGTGGTNLVQTVVTKDLTKFL (1)

SKVTEPLAEETSLSLEELFTNETGGSSP (1)

EWHDIPMRDKVLRLVARISSRVFLGPELCRNENW

LRITRDYTVTGFFAGEDLRMWPEFVRPLVHWFLPGCRKLRADVKEARSMINSVVEERRKRNQELTAAGQD

IPDYNDAIAWFDKAAKGATYDRAAMQLSLSLAAIHTTTDLLTQVLTRISQNLEILKPLREEMISVLREEG

WNKTSLYKMKLLDSVIKESQRMKPTEI (1)

VSMMRLALDDVKLSDGTVIPKNTGVAVSSHRMWDPSLHPNPHQ

WDGTRFYKMRDDPAKQNSSQLVSTSPDYLAFGHGQNACPGRFFASNEVKIALIQIILKYDFELKEGASPQ

IYKHGFTLTGDPFLELRIRRRKEEVPL*

 

>CYP68N2P pseudogene Nectria haematococca

fgenesh1_pg.scaffold_36000012

Necha1/scaffold_36:51153-51671

48% to 68J2 C-term only, 64% to e_gw1.41.77.1

remove last exon from model, add cyan

(1) VSMNRLALADVELSDGIFVPKDSSLTVLSHKMWDPKVYANPDQWDGYRFLKMREDPEKQNSALLVSTSPDY

LGLGHGQHACPGRFFASNEAKIAVLSFIIKYDFKLVGEAPPDVIKHGFNLSGDPSTQMRVRRREEEATV*

 

>CYP68 FRAGMENT Magnaporthe grisea MG10029.4 AACU01001319 cont2.1919

62% to CYP68A1 C-TERM fragment RUNS OFF END

VACTPTHFGFGHGVHACPGRFFAANEVKIALAHILLKYDWKLA

EGSEKLEPVERGLNYVVNPNIKFLIRRRKEELDLESLEF*

 

>CYP68P1   Aspergillus fumigatus Af293

          GenEMBL XP_756138.1 also EAL94100.1

          41% to 68N1, 39% to 68A1

MDFLQRILGILSSEDVAPALFASSISVLFLILSYQVLYPPSKYSAFPTINGTRWLALSNRRILAEFVTDA

QGLMRNGLEKYDIFRIISSIGPMTILHPKYTDEIHNDRQLNFMAVLAKEMFPNYPGFDLFREGTDGSTVL

QDAVKFGSSRCLGKSTQLLSDETSTLLQKLWGDEPEWHEVTAKSSVHDIIAHLSALLFYGPELCSHKEWL

EVTDEYASVGFLAARQLRLWPPILRPIAQWFLPACRRLRYLASRTRGLIEPVIAARQKEKAICYSHGRQP

PVYDDAIEWTERAAKGRPYDAAMSPLLFSINALHTTTDLLTQVILDLSTQPDLIVALRQEILSVKPQQNG

WKNASLNQLLLMDSAIKESQRLKPTESILMRRYAMDDLTLADGNKIPKGTVLGIPIFGMRDPKIYVDPDM

YDGYRFQKMRDKPGFENKCQLVSTSPWHLGFGHGIHACPGRFLAAVQVKIILCYIVAKYDFKLAGGAPPK

VQSVGIELISDTEARLAVRRRQEMVIGLE

 

>CYP68Q1  Aspergillus oryzae

         GenEMBL BAE64916.1, AP007171.1

         47% TO 68L2, 45% to 68L1, 48% to 68D1

         14 P450 genes and 2 pseudogenes on this contig

MDHFNLAGPESNTSITSLEWLGIKNSFTGSHWAHITGLSELHPTGFLCLIATLIIGIVHLTRGPKPTVLP

VVNPPGTFELTANRVKKEWLVDARQIIRRGFEKFPGKPFNMIAADVGLTTVLPPEYASEIRNNPSLSFVA

FMAHLFFSELPGFEPTREGMFDNDIGITVVHKYLTVNLARITEPLSREATAALKDIFTDNSEWHDANLKA

INLALVARLSSRIFLGEELCRNEEWLKITVNYTVDVMKAAERLRRVPGPLRRIVHWFLPEAQKCRDEVKR

AGKVIRPVLEKRRREKATMESEGKEALQYNDAIEWFEQMAKSQGTSYDPEVVQLFLSTVAIHTTSDLLTV

VMADLARNPEIIEPLREEISSVLRDGGWKKTSLTDMKLLDSVLKESLRLKPIAVVSMRRVAMDHLKLSDG

TFLPKGTKMAVSSHRMWDPDVYENPEQWDGFRYVNLRETPGQDKHAQFVSTSERHLGFGHGKHACPGRFF

ASSELKVALCHILMKYDFELAPGTVVQHRYSGASYYADPAIRVMLRRRNVALPSWFER

 

>CYP68Q2P Aspergillus clavatus

~73% to 68Q1 Aspergillus oryzae

pseudogene adjacent to CYP5117A2P on opposite strand

ACLA_064150

ISIARITEPLSQESTSALRDIFTNDPD ()

LAAQGKEAVPFDDAIEWFEQMAWSKNITYDPEIVQLFLSTVAIYTTSDLLTVTLLDFARN

PEIIEPLREEITAALEHGGWRKTSLTEMKLLDSVLKESLRLKPIAV ()

VSMRRVVMDHVKLSDGTVLTKGTKLAVSSHHMWDPSIYKDPEKWNGQRFLHRRDVPGQDK

DAVFVATSERHLGFGHGKNACPGRFFASSELKVVLCHILMKYDIELPPRAVVRHHYSGAS &

ADPEARLLIRRRSVPLPAGFE

 

>CYP68R1  Aspergillus terreus

         AF141924.1, XM_001209275.1

         lovastatin biosynthesis gene cluster

         gene="lovA"

         function="involved in lovastatin production"

         39% to 68D1 N. crassa

         73% to CYP68R2 gene mlcC Penicillium citrinum ML-236B

         (compactin) biosynthetic gene cluster

         ATEG_09960.1 4 aa diffs

MTVDALTQPHHLLSLAWNDTQQHGSWFAPLVTTSAGLLCLLLYL

CSSGRRSDLPVFNPKTWWELTTMRAKRDFDANAPSWIESWFSQNDKPIRFIVDSGYCT

ILPSSMADEFRKMKELCMYKFLGTDFHSHLPGFDGFKEVTRDAHLITKVVMNQFQTQA

PKYVKPLANEASGIITDIFGDSNEWHTVPVYNQCLDLVTRTVTFIMVGSKLAHNEEWL

DIAKHHAVTMAIQARQLRLWPVILRPLVHWLEPQGAKLRAQVRRARQLLDPIIQERRA

ERDACRAKGIEPPRYVDSIQWFEDTAKGKWYDAAGAQLAMDFAGIYGTSDLLIGGLVD

IVRHPHLLEPLRDEIRTVIGQGGWTPASLYKLKLLDSCLKESQRVKPVECATMRSYAL

QDVTFSNGTFIPKGELVAVAADRMSNPEVWPEPAKYDPYRYMRLREDPAKAFSAQLEN

TNGDHIGFGWHPRACPGRFFASKEIKMMLAYLLIRYDWKVVPDEPLQYYRHSFSVRIH

PTTKLMMRRRDEDIRLPGSL

 

>CYP68R2  Penicillium citrinum

         BAC20565.1, AB072893

         gene mlcC compactin biosynthetic gene cluster

         join(AB072893.1 :15823..16167,

         AB072893.1:16234..16405,AB072893.1:16469..17508)"

         note="putative ML-236B biosynthetic gene"

         73% to lovA CYP68R1

         note: compactin is 6 desmethyl lovastatin

  1 MLGQVLLTVE SYQWVSTPQA LVAVAVLLSL IAYRLRGRQS ELQVYNPKKW WELTTMRARQ

 61 DFDTYGPSWI EAWFSKNDKP LRFIVDSGYC TILPSSMADE FRKIKDMCMY KFLADDFHSH

121 LPGFDGFKEI CQDAHLVNKV VLNQLQTQAP KYTKPLATLA DATIAKLFGK SEEWQTAPVY

181 SNGLDLVTRT VTLIMVGDKI CHNEEWLDIA KNHAVSVAVQ ARQLRVWPML LRPLAHWFQP

241 QGRKLRDQVR RARKIIDPEI QRRRAEKAAC VAKGVQPPQY VDTMQWFEDT ADGRWYDVAG

301 AQLAMDFAGI YASTDLFVGA LVDIARHPDL IQPLRQEIRT VIGEGGWTPA SLFKLKLLDS

361 CMKETQRIKP VECATMRSTA LRDITLSNGL FIPKGELAAV AADRMNNPDV WENPENYDPY

421 RFMRMREDPD KAFTAQLENT NGDHIGFGWN PRACPGRFFA SKEIKILLAH ILIQYDVKPV

481 PGDDDKYYRH AFSVRMHPTT KLMVRRRNED IPLPHDRC

 

>CYP68R3  Monascus pilosus

         DQ176595.1, ABA02241.1

         monacolin K (lovastatin) biosynthetic gene cluster

         possible GC boundary at exon 2/3 DNKggc

         86% to lovA CYP68R1

41447  MTVPTDTVSRRLQSLAWSDIKQHAPWLPSSRTLVSGFLCLILLQILYSRGRKSDLRVYNP  41626

41627  KKWWELTTMRAKREFDANAPAWIEAWFSKNDQPLRFIVDSGYCTILPSSMADEFRKMKEL  41806

41807  CMYKFLGT  (0) 41830

41908  DFHSHLPGFDGFKEVTRDAHLITKVVMNQFQTQAAKYTKPLADEASATIADIFGDNK  (1) 42078

42144  EWHTAPVYNECLDLVTRTVTFIMVGDKLAHNEEWLDIAKHHAVTMAIQARQLRLWPVILR  42323

42324  PIVHWLEPQGAKLRAQVRRARQLLEPIIQERRAEKAKCLAQGIEPPRYVDSIQWFEDTAK  42503

42504  GQWYDAAGAQLAMDFAGIYGTSDLMIGGLVDIVRHPHLIEPLRNEIRTVIGEEGWTPASL  42683

42684  YKLKLLDSCLKESQRVKPVECATMRSYALQNVTFSNGTFVPKGELVAVAADRMSNPEVWP  42863

42864  EPKKYDPYRYMRLREDPDKAFSAQLENTNGNHIGFGWHPRACPGRFFASKEIKIMLAFLL  43043

43044  IRYDWKLVPNEPLQYYRHSFSVRIHPATKLMMRRRDEDL*  43163

 

>CYP68S1 Aspergillus niger

e_gw1.4.292.1|Aspni1

51% to CYP68L2

MELKVDVKEHGNPLSLGTSIFLLSLVLLFVFRWGDTPASQNGLPLINAKRRFEIVDLLAKKRFITNAQELLKAGLEKASV

FNIVTENGVMMVLDPKYADEIRNSKTLSLRKTLIEDMHVNVPGFEQQMQALEDDEIFQTTLKTKLTRNLEDLNEILSQEA

TVALQEHWTNNPEWHEIPLSRSMLKIIAQLSSRVFLGDKICHNPKWLRISVDYSIDLFQATFELRIYPRFLRPLASRFQP

SCRRLRADIREARSIIEPVIEERQLAKAIAKQEGRDPERHEDTIEWLEEVANGRQYNPVSAQLLLSSAAVHTSSDMLTQV

LYDLDGRQELIEALREEIRSVFENGWERSSLAKLRLMDSVLKESQRLKPISSVSMRRMAEAPVTLSDGTLIPKDCKVIVP

CLRQWDEKYYPDPETFIPDRFLKLRNEPGYEHTAAFANSSPNHLGFGLGRHACPGRFFAANEIKLALCHILMKYEFRVVG

KKPEKVVFGTMLAADPNGVIAVRRRK

 

>CYP68T1 Aspergillus niger

e_gw1.3.1460.1|Aspni1

51% to CYP68L2, 42% to e_gw1.4.292.1

MFAANIISYLAKLVPPRWIWAPILAAVFFWLLSAPSLKTSLPVINQRRRFEIGSLGSLKRFFKDAHGLVRAGLSQGSAFY

LYSEFGPKIVLASKYADDFRSHHALSLLDAVAEEYHAHIRGFDPFNRDPAIIIDMVRMKLTPHLGDLARPLSEETGRALG

ELFTDDPDWHDLELKPNMLDLISRLTSRVFLGEKLYRNPDWHRVTAGYVVHTFMSALFLRMFPRCTRPFVANLLPFCRKM

RKELQEAKDIITPVVEERRTAKQDAIRQGKEPERYVDAMQWMEECAKGRPYDPTLAQISLTLATIHTTSDLLTQTLFYLA

ERDEIIVALRNEVITVLQGDGWSKSTFNNLKLMDSILKESQRLKPHNMDVILSDGTVLPKGSISVLTTEAMRDPTIYPDP

EIFDAYRFLKLRETPGYETMVQAVSTTPQHLAFGLGKHACPGRFFAIALLKIILCHILLEYDFKIADGWSPTVRKYGFIW

QADPVAKVSVRRRKEEFTIL*

 

>CYP68U1 Mgr025 Mycosphaerella graminicola

47% to CYP68N1   Nectria haematococca, 48% to CYP68D3 A. Niger N-term from JGI model

fgenesh1_pm.C_chr_10000062|Mycgr3

MIILALAAVAVHWSDRSSN

PLLNAKGFFELSDRRIKIAFLGNARPMLSDWFGKHQNSPVSIYSDTGVLMVLPGSMANEIRNDKRFHLRQQVERMMHGRLPGFEQLGKRTVHIVSRNISLTQREAKVTTPLVDEISVAVSELFGSNSDWHELTIQTTILELVARTSSRVFLGAELCHNPEWLKVSKEYAVLAFLAAQDLRLWSPWLRPIASLFLSKCRLSRAAIHKARATIEPVLAERSKLVAKNTGGKATVFDDAIAWIECAAGTASYDPQIVQLSLSAVAVHTTTDLLSQALADIAAHPEIIAPLREEMISIMNQDGVLQTASLGRMELLDSCIKESQRMKPLSIASMGRIATERVTLSNGTVIPKGQSILVDSSRMWDAKVHPSSPKTWDGYRFLRMREDPMKRDMAPLVSTSPDHIAFGYGVHACPGRFFAANEVKIALIYILLKYELRFEEGQMPRAFDHGFTCVSDPSLKLSVRRRTEATVL

 

>CYP68V1 Histoplasma capsulatum G217B

50% to CYP68N1

HCB01530.1

MATLEPTTSYIAELISTSNLPSITFVVTALTLAVFYILQRRKSTVPLINPKRWFEFSDYRIKQEFVHNAIPLVKKGFAAT

GNKPFRILADSGEVTVLPPDVANEIKSNDRMSFELNTAKHFFGHLPGFEVFNATGQNMKVSKSIVQRQLTTHLNKVTKPL

SDEASLSLQEILTDNKEWHEITLKNELLQIIARISSKVFTGDELCHDKTWLDITVNYTIMAFAAADSLRMWPPYLRSIVH

WFLPKCRASRAEVVRARTVIEPILKRRAEQKAALAAQGKKAPEINDAIEWFTSATTIEGFTLDPVIAQLGLSLAAIHTTG

DLSTQVILDIASHPEIIEPLRKEMIESLSEGGWKKNSLYKLKLLDSVIKESQRMKPIASISMNRLTTTNVTLSDGTFIPR

NTATAVSSHRMWDPSVHTNPDQWDGYRFYNKRQEPGQENLSQLVSTSPDHLAFGHGQHACPGRFFAANEIKVLLCHLLLK

YDLKIVEGSMIKPFPYSFSMNANPFAPLMIRRREDVIDLDALDA

 

>CYP68W1 Aspergillus clavatus

42% to 68D1

ACLA_055740 removed small intron

MFRENLGPIFLTALPAILLSYFLSVALPSLRLQWKTSHIPIINKKKGEWSDSNAVKRCAHNAQAILQEGHEK (0)

YTGPFQLIGSFGRRIVLPPELGEHVKTDPRFTASEVVRQTMAGSLPGFEGLLPVMEAGAPNVFIQMIRS

KLTSNLGKFTQHLAEETQAAFDEKWGTSTEWHEVDLLASLRDIVTQVSTRIFLGPELCKNKEWLDNTQAYTDNVFKAVRS

IRRFPAWSRPYVQWFLKDCSRIRQQVKRAEEIIEPIIAKRLEEIALAQKGLAEMPNDAITWMHQVAQGKGKDYHGAHIQL

SLSMASVHTTSDLFSNILLQLCRHPEWIEPLLQEAQSVRSEGGWEKTSLYRMKLADSVMKETQRIKPLGVLLSHRHVSDD

VILPDGTLIPKGAIVATNMSRMWDPEFYPNPEEWQPDRYLKRREEPGKEHSSQFVTTTMESLGFGIGSHACPGRFFASSK

IKLLLNHVLQNYEFERVDTGKDATPFFVEYVTNPKAKLRVRRKEISS*

 

>CYP68X1 Neosartorya fischeri

47% to CYP68V1 Histoplasma capsulatum

NFIA_098910 revised

Note: this seq does not have an ortholog in A. fumigatus

MAVQEIITILLKHIHSAGL

MAWLLLVLFVGSVMLTIRKSLSTNGA

SHIPLIDPRKSYDIGGIYAKIDFILHARRLLALGVSSGRPFRLLTDLGEMIVLPACFAAEIRSDHRLSFAE (0)

NFHSRFPGFEGFRMGTTDALLSRDIANNQLTHTL (1)

AKVTQALSDECAAALNDHFPAKE (1)

ENRMPEWSDICLREHVLQLVARLSSRVFLGDEGANNRAWLR

ITTEYTKTAYIAAYMLRLWPPFLRPFAHWFLPHCQKLRGQVAEARRIITGVIERRRESIAAAELAGQQIPQYNDAIEWFE

QDVAKQDGRYDPVVAQLILAQAAIETTTDLLTQVILDLAQHPELADPLREEVTKIIREGGWRKSSLYEMKLLDSVLKESQ

RLKPLAMTSMHRLVLEDITLSDGTRLPKGSVIGVSVDRMWDPSVHENPTQFDGFRFQRMRDHPGGTANQAHLVSTSVNHL

AFGHGKHACAGRFFVAHETKIALTHLLLKYDWRIASSSIDARPMEFGLVLQANPKAKISIRARQPDSEGPIRA*

 

>CYP69A1 Gibberella fujikuroi P450III gene Y15279

MKYTTCQMNIFPSLWSMKTSFRWPRTSKWSSVSLYDMMLRTVAL

LSGRAFVGLPLCRDEGWLQASIGYTVQCVSIRDQLFTWSPVLRPIIGPFLPSVRSVRR

HLRFAAEIMAPLISQALQDEKQHRADTLLADQTEGRGTFISWLLRHLPEELRTPEQVG

LDQMLVSFAAIHTTTMALTKVVWELVKRPEYIEPLRTEMQDVFGPDAVSPDICINKEA

LSRLHKLDSFIREVQRWCPSTFVTPSRRVMKSMTLSNGIKLQRGTSIAFPAHAIHMSE

ETPTFSPDFSSDFENPSPRIFDGFRYLNLRSIKGQGSQHQAATTGPDYLIFNHGKHAC

PGRFFAISEIKMILIELLAKYDFRLEDGKPGPELMRVGTETRLDTKAGLEMRRR

 

>CYP501A1v1   Candida albicans

             GenEMBL XM_706447, orf19_1411

             allele of CaO19.8987 XM_706426.1 (5 aa diffs)

             best hits are to CYP504, but only low 30% range

             in CYP64 clan

             putative phenylacetate hydroxylase

MLSSIRWDEALLILLNYLNDYPVTITCFLVLLIIALGIAIDYII

TPKEIANIFPIPGELPFIGHLHLILENPALIYLTWSKLYNQSIFQIRIGNKRVVVVNS

FDDVVGLWVNHSCQNNSRPLSYTFHDLVSAIQGFTIGSTPASLTFQKKKKTISQYLNK

RQIELKSDTINRQINIMIGELWKRDIEFEDINLLPYFQKFVLQTAIFIGYGIELDCYK

EHSQFCQEIIDIENKIIRLRSPISNLQDSIPLLRIMPRWFNNSEIARMCGERRNSYMN

KLYQQLQQGLAEDLPQYKNSILGDLITKTTNTQNNNDDSSDTNCTSHNNCLNEQEIQS

ICLTLISAGLDNTPLSLNYLFGILSHPKIGEHLQNRAIQDILEDSGGNIVLAWEKVSS

LQGQMTTNQQNPMNCQYIEALVLETLRHFTVLPLSLPRLTTKAIRYKDFVIPKETHLF

MNAYSANHDCQIFVNPYKFDPDRWLDPVSNSIKSQFLNFIINNNSKSSSGHGHQFKSL

HSHQHFAFGAGSRMCSGYNLVIKQMYVMIIKMLLIFEIHPPTNIKKLMELNPFKNNSN

PKGTSFEPKTNHINLKFRKLPNYKSLHELVLHQS

 

>CYP501A1v2   Candida albicans

             GenEMBL XM_706426.1

             allele of CaO19.1411 XM_706447 (5 aa diffs)

             Gene sequence has no introns AACQ01000193.1, AACQ01000194.1

             best hits are to CYP504, but only low 30% range

             in CYP64 clan

             putative phenylacetate hydroxylase

MLSSIRWDEALLILLNYLNDYPVTITCFLVLLIIALGIAIDYII

TPKEIANIFPIPGELPFIGHLHLILENPALIYLTWSKLYNQSIFQIRIGNKRVVVVNS

FDDVVGLWVNHSCQNNSRPLSYTFHDLVSAIQGFTIGSTPASLTFQKKKKTISQYLNK

RQIELKSDTINRQINIMIGELWKRDIEFEDINLLPYFQKFVLQTAIFIGYGIELDCYK

EHSQFCQEIIDIENKIIRLRSPISNLQDSIPLLRIMPRWFNNSEIARMCGERRNSYMN

KLYQQLQQGLAEDLPQYKNSILGDLITKTTNTQNNNDDSSDTNCTSHNNCLNEQEIQS

ICLTLISAGLDNTPLSLNYLFGILSHPKIGEHLQNRAIQDILEDSGGNIVLAWEKVSS

LQGQMTTNQQNPMNCQYIEALVLETLRHFTVLPLSLPRLTTKAIRYKDFVIPKETHLF

MNAYSANHDCQIFVNPYKFDPDRWLDPVSNSIKSQFLNFIINNNSKSSSGHGHQFKSL

HSHQHFAFGAGSRMCSGYNLVIKQMYVMIIKMLLIFEIHPPTNIKKLMELNPFKNNSN

PKGTSFEPKTNHINLKFRKLPNYKSLHELVLHQS

 

>CYP501A2   Candida tropicalis MYA-3404 cont1.80

           GenEMBL AAFN01000033.1

           62% to 501A1 in CYP64 clan

           putative phenylacetate hydroxylase

128977  MLLSIPWDQSLLTLLTYLDTHPIATIFTIILTILTIGILFDYCLSPKEIANIFSIPGDLP  128798

128797  FIGHLHLILDNPALIYLTWYKLYNKLVFQIRIGNKRVVVVNSFDDVVGLWINHSCQNNSR  128618

128617  PLSYTFHGLVSALQGFTVGSTPASLTFLRKKKVISLCLRKKEIDEKVCLIDNEICVMIKE  128438

128437  IIKKKDISTDVNMLPYLQKFILKTAILMSYGIELDCYNKDVKLCQEIITVENNIIRLRSP  128258

128257  ISNLQDSVPFLRLIPWFNNREFALRCGNRRNKYMDQLYNRLQNGLAENDPNIANSILGQ  128081

128080  LILNNDNNNSNSLTSQEIQSICLTLVSAGLDNTPLNLNYLIGILSQ  127943

127942  PRIGKIFQDKAIKDILNHANGDIIQAWNQLNEENRDCKYIQALILETLRH  127793

127792  FTVLPLSLPRLTTKPIYYKNFMIPKNTHMFMNAYSANHDELIFKNPFKFDPERWLDSETN  127613

127612  EIKSKILATTSSSSSSTHHGGGNGINVQNFHFAFGAGSRMCSGYNLVMKEMYMMIIKL  127439

127438  LLLFEINPPDNNNNNGKYLMEMNPFVNNLNPRGTSFEPRIHNIKLQYRKLPNYETLHEIVLK*  127250

 

>CYP501A3   Candida dublinensis

89% to CYP501A1v1     Candida albicans

cdub_11-g248.1

VSNNIKSQFLNYIINNNSNRSNHGRGQ this seq may bw an intron (too long) remove it

MLLSIRWDEALLILLNYLNDYPVTITCFLILLTIVFGIVIDYTITPKEIANIFSIPGELPFIGHLHLILENPALIYLTWS

KLYNQSVFQIRIGNKRVVVVNSFDDVVGLWINHSCQNNSRPLSYTFHDLVSAIQGFTIGSTPASSTFQKKKKTISQYLNK

RQIELKLDIINRQINIMIGELWKRDIEFEDINLLPYFQKFVLQTAIFIGYGIELDCYKEHNQFCQEIIDIENKIIRLRSP

ISNLQDSIPLLRIMPRWFNNSEIARICGEKRNIYMNKLYQQLQQGLADDLPQYKNSILGDLITKTKNRNDNGESGGMNCI

SRNNCLNEQEIQSICLTLISAGLDNTPLSLNYLFGILSHPKLGERLQTRAIKDILENSGGNAILAWEKVLLLQALVTTNQ

KNLMNCQYIEALIMETLRHFTVLPLSLPRLTSKEIRYKNFVIPKETHLFMNAYSANHDCQIFVNPYKFDPDRWLDP

FKSHHNHQHFAFGAGSRMCSGYNLVIKQMYVMVIKMLLIFEIHPPTNTKKLMELNPF

INNLNPKGTSFEPKTNQINLKFRKLPNYKSLHELILQQD

 

>CYP501A4 Candida parapsilosis

50% to CYP501A2     Candida tropicalis

CPAG_01739

MPWTTVDGILLYIHSIARSPLTRWTCFVSVALLMIAIVYDYIYKPSKIKGIYPIPGELPIIGHLRLLRNNPSQVFLVWAT

RYDKSVFQIRLGNKRVIVVNDYDDVVELWMNHSCQNNSRPLSYTFHGLISSYQSFTVGSTPQSTTYKRKKRAIAQFLNSK

SISSYSLSICREIELTLFRLENVAINVDVDVCHYLKYFVLRCSVFLMYGLHLDCFGVDNALCNEIISNESEIIRMRSPIA

NVQDSVPLLKYLPNLTNASLAVQCKNKRERYMSELFARFEDKMNLGDCDSIGSFIGQLIAETGNKQLTDAEVYSICLTFI

SAGLDNTPLNLNYLLGILSQPSIGKAYQSKAINEILANCGGDVQKAWEQSNESTLKNVYVHALILETLRQFTVLPLSLPR

TTTKTIIYNGVEIPAGSQLFMNAYAANHDPKHFKCPFEFNPERWIDANGQISFTAKAYQHFSFGAGSRMCSGYSFAVKEM

YIFMVKFLLMFEVHPPDSKLMIADPFLSNENAHATSFEPRSHNIRLTLRRSRNQEWLHDTELVR

 

>CYP501B1 Candida lusitaniae

38% to CYP501A3 with some indels removed

CLUG_05046.1

MWLALLLLVALLFFLPPPRPIVGAFYVPGYPLVGNACQVLHNPSRVFLSWAQTCGHALFVIHLGRTPVLVVNSHADVHDL

WLLHSSALGSRPTLHTFHNVVSAVHGLTVGSTPAGASYRRKKKTLSSELSASRVQSKV

LSGNAQRALLSPNLSSVPKSSSIDSGHESGFPPILSHIDVCSSECIAQLLSHPA

ETSLLLETQYFVLSAAIRLTYGIVLDCHGRDASLANTIIATENKIIRM

RSLVANYQDYIPGLQSFPLRCMFDARAAKWRQQRDSYMHSLYQSFLVQLHGGSFLANHSMLAHILRAKASPMTLSESELQ

SICLTMVSAGLDNSALTFDHLMGHFSHDYGQDMQERLWKELMLQSNGSLATAWDAATYGTCSYAMALLHEALRFFSVLPL

GLPRQTTKPVRFRGLYIPANTIVVMNNYAANHDPAAFESPFEFSPDRWLDP

NGNLITPNHFSFGAGSRKCAGSHLALKEL

HTLLCRMVLLFRIKPPQDARFKMCLDPFEGNSCPTGTSFEPKEFRVRLEPREGKECDELRDRLRDYRIPQERAK

 

>CYP501C1 Candida guilliermondii

43% to CYP501A2, yellow is long

PGUG_01534.1

MSSLWYLVTDCILLFIFAWIIEDCILALGVEPPIPGIKQIPSYPIVGNLLQVKNNPAKVYLKWSKFYKVPVFQVRLGFRN

IVVANSYQSVEFLWGKSASANNSRPTSHIFHNIVSQSQGFTIGSTPASSSLFKTKQALSSELNRKSVNMIAEQLDQQVKY

TLRMIIRNNVELK

GSPSTFLSHVRSRLS

DIDLLPYFQLFALRSAIQLTYGITLDCYGKHGRISQEIIEVENQIIKLRGQG

SWLPDFLPFLKYFGNWELSASKYRLRRDNYMNELSRDFKKRYSNNDAAARTSLLGRLYKNSSHLAHHELNSICLTMVSAG

LDNTPLTINHLMGHLSRPDYGYALQQVAFSQLLNCYGDINDAWNNCGTNMECNFVVALVQEALRFFTVLPLSLPRKTTKV

IKFNGVVIPEGAVLYMNAYAANHDSEKFSNPMKFDPFRWLVPRTMKLKDRREMDHFAFGAGSRKCSGNILATQEMYSMIC

RLILIFRIRRPIEEAMELDPFESNACPSATSFEPKRFKVHLEPRCHEGSEVLYRSLQ

 

>CYP501D1 Debaryomyces hansenii

45% to CYP501A2     Candida tropicalis

CAG86740.1

MVFATMGSAIFDFIRLLITFWNYKILNLLNIIFAIFLLSIFMDLVLGIGVPLKIKGIFAIPGYPIVGNLFQVLNNPALVY

IQWANKYNASIFQIRLGFKRIIVVNSFEDVNILWTKHSCSNNSRPVQYIFHGIVSATQGFTVGSTPSGPSYKRKKKVISQ

SLNQKSVNNLSSVIDNETKFTIKNIIQNNRELFGPPSCNNYRYTRTIISDIDLLCYAQLYSLRSAIFLTYGLHLDVYDSE

ADLAKEIIEVESNIMKFRAPVSNLQDYIPILRYLPFGKSMQAQHYRNRRDAYMNKLFQQLQEKILEGDVDSSNSLVGVAI

KNMELTLKLTSAELQSICLTMVSAGLDNTSLNFNHLMGQLSQPTTGYIYQRKAFEEMMSRNKNNICTAWDNAAIVMDCSY

VIALIKETLRYFTVLPLGLPRITTKDITYKDAIIPKNSILFMNSYAANHDPNIYKDPMRFIPERWLDLITGLVLSKYEIS

HFSFGAGSRMCSGNHLAFKELYTLTCRMILTFQIRRPTSNKFLMELDPFKNNSNPNAISFEPKLFKVRLEPRIHNRSDDL

YNKILRS

 

>CYP501D2 Pichia stipitis

56% to CYP501D1   Debaryomyces hansenii

JGI model e_gwh1.7.1.272.1

ILGVFKIPGYPIVGNLFQVINNPAQVFMKWSEVFNVSLFQIRFGSRSVVVVNSFEDVTRLWIKYSCSNNSRPVLYTFHQV

VSLTRGFTVGSTPAGESYKRKRKSISQHLNHRAVERSKSTLDKEVEYMINQMISRNHELSGPPSVNMFRNSKCELADIDM

KIYFQQFALRSSVFFAFGIHLDCFGKDSKLCNEIIEVESAIMKFRSPIANLEDFLPILRYFPGKFKKAAEFRERRDRYMN

KLYKTMIEGIVVGNADAVASIVGTIKIEQDQQMNKLTEEEIQSICLTLVSAGLDNTALNINHLMGHFSQPFYGAALQKKA

YDALLAGSNGDICQAWDDAVDVKCPYVIALVQESLRYFTVLPLGLPRITTKDIVYNGAFIPKETILIMNAFAANHDSCVF

QSPYEFIPERWLDAETGELIDKHDLIHLSFGAGSRMCAGIQLAINEMYTFTSRLVLYFKIKPPTCGAIMDLDPFRNNENP

SATSFEPKPFKVRLEPRYSDSLYNKLRRS

 

>CYP502A1 Coprinus cinereus AB013443

MTTTSSSIFAGLGCLLVASIIYFRKFKANPARAHLPPGPKPIPV

LGNVKDLRAKELWLPAMDWAKQYGDITYLHVFGQGLTFINSLESASDLLEKRGGMYAD

KPQFTMVCELCNCKNMVAFTPYGEQSKRQRRLMHKAFAPARIPDYHPLMESSTNLFLR

NVIASPADYIGHVRRYSGSLTLNIVYGYEVTSNEDEYLMMAEECVGILANEIASAGGV

WAVDVMPFLAKIPKWAEGLPGMSFKRKARKWKKMMEDWVDGPFEYVKNTMKSGTYKQS

FCSSLLDDESISQTQEHFEFDLKWTANSMYAASIDTTITSVAHFLLAMMKHPEVLKKA

QHEIDTVVGQDRLPTFSDRKSLPYVEAVLSETWRWASPVPLSLPHKLTEDDVYRGMYI

PKGSLIFANIWAMTRDERIFPDPETFNPERYLNMDPETKKKQDPRNFIFGFGRRLCPG

NHIVDASLWLLVVRMMATLDISTPVDEKGNAIDIVPVFDNPIFRTPNPFPCDMRPRSE

KAVNLIRQYADPRASA

 

>CYP502B1 pc.5.187.1 Phanerochaete chrysosporium

MDTVLVGLFVALALYAWSRSSKRSALPVPPGPKPVPLLGNIFDLTAKELWLRVTGWSKQYDIVYIHLLGQGLVFCNTYEVAQDLLEKKGSIYSDKCGCQNMVAFTRYGDFARRQRKLMNTAFGISAVKRYRPLLANESVLLLKRILADPQDYMGYIRRYAGGLTLQSVYGYRVETNDDPLLELGTECVDILSNKIASGGGIWPVDIFPFLQHLPTWFPGAGFKRKAAVWRAKMEEFVDKPYEMVLERMRSGATVPCFVTTLLEEARDEKGGAVDAQRDFDIRWTANSMYSASMDTTITVVQLFLLAMILHPEVLRKAQAELDAVVGPARLPTFADRPALPYLDAVMSEVLRWGVPVPLGLPHRLMEDDVYRGTHLRAGTLVFANIWNMLRNEAIWAQPDVFRPERFLEPVDEATAKRRDPRPYVFGFGRRRCPGLHLIEESLWIVMATLLATTDILAEKDESGKPVMPHVDFTNSLVVPFSTPAPFKCDIRPRSEQALQLVRLAE

 

>CYP503A1 Gibberella fujikuroi Y17243

MSKSNSMNSTSHETLFQQLVLGLDRMPLMDVHWLIYVAFGAWLC

SYVIHVLSSSSTVKVPVVGYRSVFEPTWLLRLRFVWEGGSIIGQGYNKFKDSIFQVRK

LGTDIVIIPPNYIDEVRKLSQDKTRSVEPFINDFAGQYTRGMVFLQSDLQNRVIQQRL

TPKLVSLTKVMKEELDYALTKEMPDMKNDEWVEVDISSIMVRLISRISARVFLGPEHC

RNQEWLTTTAEYSESLFITGFILRVVPHILRPFIAPLLPSYRTLLRNVSSGRRVIGDI

IRSQQGDGNEDILSWMRDAATGEEKQIDNIAQRMLILSLASIHTTAMTMTHAMYDLCA

CPEYIEPLRDEVKSVVGASGWDKTALNRFHKLDSFLKESQRFNPVFLLTFNRIYHQSM

TLSDGTNIPSGTRIAVPSHAMLQDSAHVPGPTPPTEFDGFRYSKIRSDSNYAQKYLFS

MTDSSNMAFGYGKYACPGRFYASNEMKLTLAILLLQFEFKLPDGKGRPRNITIDSDMI

PDPRARLCVRKRSLRDE

 

>CYP503A1   Gibberella proliferatum (teleomorph sexual form)

MNKSNSMNNTSLERLFQQLVLGLDGIPLMDVHWLIYVAFGAWLC

SYVIHVLSSSSTVKVPVVGYRSVFEPTWLLRLRFVWEGGSIIGQGYNKFKDSIFQVRK

LGTDIVIIPPNFIDEVRKLSQDKTRSVEPFINDFAGQYTRGMVFLQSDLQNRVIQQRL

TPKLVSLTKVMKEELDYALTKEIPDMKDDEWVEVDISSIMVRLISRISARVFLGPEHC

RNQEWLTNTAEYSESLFITGFILRVVPHILRPFIAPLLPSYRTLLRNVSSGRRVIGDI

IRSQQGDGNEDILSWMRDAATGEEKQIDNIAQRMLILSLASIHTTAMTMTHAMYDLCA

RPEYIEPLRDEVKGVVDASGWDKTALNRLHRLDSFLKESQRFNPVFLLTFNRIYHQSM

TLSDGTNLPSGTRIAVPSHAMLQDSAHVPGPTPPTEFDGFRYSKIRSDSNYAQKYLFS

MTDSSNMAFGYGKYACPGRFYASNEMKLTLAILLLQFEFKLPDGKGRPRNITIDSDMI

PDPRARLCVRKRSLRDE

 

>CYP503A2 Aspergillus niger

e_gw1.4.275.1|Aspni1

54% to CYP503A1

MNKAETPSDSFSELHLIAAIGLSPALVGLLLGIYSLWYLVGPALKQHNTSNQPPIVGCRSRWEPTWLVRLRFIRGSRKII

GEGYERFKDTMFQVRRVGTDVLLVSSKYMDEIRTLTRDATGSVPPLVQDYPGEYTHGMPFLRSDLQNRVLQQKLTPNLPS

LVPVMVEELEFAMRMEMPECAADDWTTVDIMPILSRIITRITSRIFLGPKEGRNEEWLVATVEYTRNFFLTGMALRFFPR

FLRPLVAPLLPSYHHLWQNVATSRRIVGEIVRERREIEAAKPADYTKPADILQWMMDAATGQEAEPGDLAQRMLILSVTS

IHTTTLTMTQAIYDLCAHPQYLEPLRAEVEDVLCTDGGWGKLTVNHLRKMDSLLKESQRFNPVFLLTFNRILPKSITLSN

GVSIPVGSRIAVPSNAMLNDPSRVPGDAPTSFDAFRYSRLREEPENAQRHLFVMTDHNNLAFGYGKFACPGRFYAANEMK

MMLAHMLICYDMRFPDGRGRPRNFTIDSDMYPDPATRLLIRKR

 

>CYP503B1 Aspergillus nidulans AN1598.1 45% to 503A1 in 54 clan

revised 7/18/07

MDNYTWHSGTLIPSDSPSSIDRSQLYLEILGVLSVVYLLQT

LVAYSKSFKAPFVGFRFWYEPKWLVGLRFSQGALAQVNEGYAKYKNAMFK

VARNDSDILVIPNKYVEELRSLPDEKISAIRAHIKNLLGKYSTTLILLES

DLHTRMLQTKLTPNLGSFIEVIESELLFAMDQEIPANLDDWQSVNVFHIV

LRIVARISARVFLGVPACRNEEWLQTSIHYTENVFATVMLLRRFPKWMHP

IVGHLLPSYWAIHRNLRTAKRIISPMVRQRRAEEAKRNPDYVKPNDLLQW

MMDGANENDGQPDKLAHRQLLLSLASIHTTTMAAAHCFYDLCQHPEYFEP

LREEINDVIAQDGGWKKTTLNKMRKLDSFLKESQRINPPSLLAFNRIVSE

DLTLSDGTLLPKGTHFSMPSAAILQDNGVEPGADQFDGFRYYKKRLNPEE

ANKHQFAMTDNNNLHFGHGKYSCPGRFFASNEIKIIMAHLLTDYEFKYPR

GATRPRNLTADENLYPDPSARLLMRRRVVAPPQASITPQLVSA*

 

>CYP503B2 Uncinocarpus reesii

54% to CYP503B1

UREG_02869.1

MPLFENTLLRNMQSTLNDTWLKLSCQKPAVFVFGVTVFLSWQLVNRIFFSVKAPYAGYESSWEPIWLISRRCSHEAPRLI

QEGYRRYRDAMFKIAKRNSDLLVIPKKFVEELRSLPEEQICAMEAHIRTLLGPITGTDILLNGHLHNKALQTRLTPNLGS

LVQPLKDELDYAISVELPNCKDEWVEVQMHGIVRRLVARLSARAFTGPVACRDEDWVMTNTMYPANVFTTIGPLRLFPSF

MRSTVALFLPSYWQLRSNFATANRVLVPIILQRRRDEASGDLNYEKPKDLLQWLMDLAEPNEAAPHLLAHRQLALSLGSL

NTTTTAAVQTLYDICDHPEYLESLRTEVLGALTADGGWDRTTITKLVGLDSFMKESQRVNPPSYVSFNRVVRKSLTLSDG

TRLPKGTHFGMASYSILQDPAIIENPHEFDGFRYKRIREDPKEANKHQFASTDSNNIHFGHGKYACPGRFFASQIVKMII

GHLILKFDFKFPEGQGRPRNLSIDENVYPDPSARLMMRRRQMVY

 

>CYP503B3 Coccidioides immitis

74% to CYP503B2   Uncinocarpus reesii

CIMG_03089.2

MFSLDPVFLNNAHILFHNAL

FTLSSNKPAIFVISAAVLLLWRLLKDILFSVKAPYVGYESSWEPTWLISRRFSREAPGLIEYGYQR (0)

YRDSIYKLSRRDSDILVIPHKFVDELRSLPEEHICA

MEAHIQTLLGPITGTDILLDGDLHTKVLQTKLTPNLGHLIGPLKEELEFAMSMELPDCKDEWVEIQMHGIIRRFVARLSA

RAFTGPIACRNEEWVAANTAYPTNIFTTIGPLRMFPSFMRSSVAPFLSSYWRIRSNIATAKRVIVPIINQRRRDEASGDP

NYEKPKDLLQWMLDTAKPKDAAPASLAHRLLALSLGSLNTTTTAAVQTLYDICNHPEYFEPLREELLDSLTANGGWDKAT

LSKLHGFDSFMKESQRVNPPSYLSFNRVVRKPLTLSDGTRFPKGTHFGMPAYSILQDPSIVENPTEFDGFRYKKIRQQPK

EANKHQYASTDSNNIHFGHGKYACPGRFYASHVVKLIVGHLILEYDFKFPEGKCRPQN

LNIDENVYPDPSATLLIRRRQMA

 

>CYP503B4 Neosartorya fischeri

92% to CYP503B1 A. nidulans

NFIA_009830

Note: this seq does not have an ortholog in A. fumigatus

MDNYTLLSLHTGNLKPPDSSSSIDRSQLYLGVIGVLSVIYLLQTLVACTKSFKAPFVGFRSSFEPKWLVGLRFSQGALAQ

VNEGYEKYKNAMFKIARNDSDILVIPNKYVEELRSLPDEKISAIRAHIKNLLGKYSTTLILLESDLHTRMLQTKLTPNLG

SFIEVIESELHFAMEKEIPADLHDWQNVNVFHIVLRIVARISARVFLGVPACRNEEWLQTSIHYTENVFATVMLLRRFPK

WMHPIVGHLLPSYWAIHSNLRTAKRIISPMVRQRRAEEAKGDPTYEKPNDLLQWMMDGANENDGQPDKLAHRQLLLSLAS

IHTTTMAAAHCFYDLCQHPEYFEPLREEINDVIAQDGGWKKTTLNKMRKLDSFLKESQRINPPSLLAFNRIVSEDLTLSD

GTLLPKGTHFSMPSAAILQDNAVEPDADQFDAFRYYKKRLNPEEANKHQFAMTDNNNLHFGHGKYSCPGRFFASNEIKII

MAHLLTDYEFKYPPGASRPRNLTADENLYPDPSARLLMRRRMVAEGQAQITPEIVLV*

 

>CYP503C1 Aspergillus niger

e_gw1.10.655.1|Aspni1

42% to CYP503B1

MVNDTELWTLVTTAHPLPVESIHWTYTLLFLAVGAYCCSIFWSQIFGYKLPIFGTKSPWEPVIISNFRFFHHAEQVLQQG

YNECKNRIFQFRRADTDMLVLPPKYVDEIRRLPNHIASPTLAHVHNLMGSRINMHIILRNNLHFRTLQVKLTPNLNSITL

PMQEEVNYAIEKDFTKSEDEWVTIKPYHTILDLVSRVSARIFLGQPLCRNPQWLELSTQFTENVFVSLVFLRLFPMWTHG

LLTWFMPSSYRGSAYIRQAKKLLVPEINRRRKLASEGIEEPAENKHNLLSWMMEIATPEESDPASLAHLEVVLSLAAIHT

SQMNAVHVLYDLLVHPEYLEPIRDEIRTTILENGPWMTWEKSAFSKLRKLDSFMRESQRVNPPTLLSMHRVLLQEVTLSD

STRLPKGAHISMAVNAIQNDPEVTTNPETFDGFRYYKLRQREGEAHLHQFSTTQDRILNFGHGANACPGRFFAALEIKII

LVRLLMDFEWKLKEPGKRPENLHAHEFIFPNPDAEILMRRRPASECLQL*

 

>CYP503D1 Mgr045 Mycosphaerella graminicola

51% to CYP503A1, 45% to CYP503B1 yellow is alt N-term from JGI model e_gw1.1.487.1|Mycgr3

MLHSTNLSTSAVTRYNDSIESALLASVVFILGVACIAAVVRPKSTIGKIPIAGQRSIFEPDWFARLRFIRSSRDIIHDGYSKAGQ

YRDIFIVRKSGTDIVVLCDPKLVDEVRA

QTKEKARSVEPFLHDFVGAYTGGKVFAESDLQNRILMQKVTPNLGAFIPIIEDELQYALDMELPKTDNTGWLEVDMVSAF

PRIVARIIARVLLGPKGCRNEAWLQTTARYTRNVFVTGFVLRFVPRFFRPLIAALLPTYWELLRDLAKARSIIGGLASDR

SSNPQEDVLQWIMDAANVDERKLDDLSQRMLVLSLSGIHTTAMTMVHAIYDLCARADDCSIIRDELSNVLNSGNGINKGT

LLEMHKLDSLIKESQRLNPVFLLTFNRILPCAVFLSNGITLPAGTRVAVPQHAILNDPAKVPGENPHCYDPWRYAKLRED

PDKAHQYQFAMVDSKNMAFGYGKYSCPGRFFVATEIKIILAHLLLKYDWKLPDGRRRPQNFTIDSDMYPDLSARVLIRRR

TDEGPLS

 

>CYP504A1 Aspergillus nidulans AN8078.1 64 clan

Emericella nidulans phacA gene, AJ132442

MSLQTIGIAAVAVVYFLIRYFNRTDIPKIKGLPEVPGVPIFGNLIQLGDQ

HATVAQKWAKKFGPVFQVRMGNKRVVFANTFDSVRQLWIKDQSALISRPT

FHTFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPATQSYMPIIDL

ESMSSIRELLRDSANGTMDINPTAYFQRFALNTSLTLNYGIRIEGNVNDE

LLREIVDVERGVSNFRSTSNQWQDYIPLLRIFPKMNREAEEFRVRRDKYL

TYLLDVLKDRIAKGTDKPCITGNILKDPEAKLNDAEIKSICLTMVSAGLD

TVPGNLIMGIAYLASEDGQRIQKRAHDEIMKVYPDGDAWEKCLLEEKVPY

VTALVKETLRFWTVIPICLPRENTKDIVWNGAVIPKGTTFFMNAYAADYD

ETHFTNPHAFEPERYLTASSDGSGTPHYGYGAGSRMCAGSHLANRELFTA

YVRLITAFTMHPAKRAEDRPILDAIECNAIPTALTTEPKPFKVGFKPRDP

VLVRKWIAESEERTKHLN*

 

>CYP504A2v1 Penicillium chrysogenum strain Wisconsin 54-1255

           GenEMBL AF056978.1

           phenylacetate hydroxylase (pahA) gene

MAIQTLAVAVITVVYFVIRYFNRTDIPKIKGLPEIPGIPIFGNL

LQLGDQHATVTGKWAKKFGPVFQVRMGNKRIVFANGFDSVRQLWIKDSSALISRPTFH

TFHSVVSSSQGFTIGTSPWDDSCKKRRKAAATALNRPAVQSYMPIIDLESNSSIKELY

RDSQNGKRDVNPTAYFQRYAFNTSLTLNYGFRIEGNVDDTLLHEIVDVERGVSNFRST

SNNWQDYIPLLRIFPKMNNEAADFRGRRDKYLTYLLDMLKDRIAKGTDKPCITGNILK

DPEAKLNDAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQRIQKKAYDAIMEVYP

DGDAWEKCLVEEKVPYVTALVKEVLRFWTVIPICLPRESTKDIQWNGATIPAGTTFFM

NVWAADYDEDHFKDADKFIPERYLEASEGAGTPHYAYGAGSRMCAGSHLANRELFTAF

IRLVTAFNMHTAKETADRPILNAIECNLIPTALTTEPKPFKVGFSARDPKKLEQWIAE

SDERTKDL

 

>CYP504A3v2 Aspergillus terreus

           AAD34565.1

           6 aa diffs to 504A3v1

           ATEG_09973.1 6 aa diffs

  1 MTLQIIVIAA TAVIYFLTRY FNRTDIPKIK GIPEIPGVPI FGNLIQLGVK HATVARKWSK

 61 EFGPVFQARL GNRRVIFANT FESTRQLWIK EQSSMISRPT FHTFHGVVSS SQGFTIGTSP

121 WDESCKRRRK AAATALNRVA VQSYMPIIDL ESMASIKELL KDSQGGKIDI NPTPYFQRFA

181 LNTSLTLNYG YRIEGNVNDQ LLREICEVQR GVANLRSTSN NWQDYVPLLR LFSNRSNQAK

241 HLRARRDKYM AFLFDILKDR MAKGTDKPCI TGNILKNPET KLTDAEIKSI CLTMVSAGLD

301 TVPGNLIMGI AYLSSEDGQR IQQKAYEEIM SVYPNGDAWE RCLVEEKVPY ITALVKETLR

361 FWTVMPICIP RVNIKEVIYN GARIPAGTTF FMNAWAANYD EDHFDMPNRF LPERYLEPSE

421 GFGTPHYSFG AGTRMCAASH LASRELYTVF LRFIVAFTIE PAQNPADMPV LDAIECNATP

481 TSMTTEPKPF KVGFKPRDET SLRRWIAESE ERTKEL

 

>CYP504A4 Magnaporthe grisea MG04684.4  71% to CYP504A1 AACU01001145 cont2.876

MQSFVVIAGLLAAAYFVHRLLYGTDTPHIKGLPEVPGLPLFGSLIELGDN

HAKVAQRWAEKYGPVFQVRMGNRRIVFANTFASVRHLWITNQSALISRPK

LHTFHTVVSSSQGFTIGTSPWDDSCRRRRKAAATALNRPAVQSYMPLIDL

ESTVSIRELLQDSRGGAVDLDPRAYWQRYALNTSLTLNYGYRIDGDKDDE

LLREITDVERGVSNFRSTSNNWQDYVPLLRLFPKMSREAVEFRERRDVYM

DRLLRGLKERIERGMDKPCISGNILKDPEAKLNEAEIKSIGLTMVSAGLD

TVPGNLIMGIAYLASPAGQEIQQRAYAEILKTYPANDAWTRCLDEEKIPY

ITALYKEILRFFTVIPICLPRVSVKDVEWEGVTMPAGTTFYMNAYAADYD

AAHFSDPYVFNPDRYLEDVEGTPHYGYGAGSRMCAGSHLANRELYTAFVR

LISAFEFVPPADPADEAVLDCLEANAIPTSLTMEPKYFKVGFRPRDRDVL

DGWIRESEERTRHLM*

 

>CYP504A5 Fusarium graminearum AACM01000162.1 revised GC boundary at KLNE old name = 504A3

MSDQILYVTVLVGLLLLYRLANSTDKPKIKGIPEIPGVPLFGNLFQLGTD

HARVARKWVAKYGPVFQTRLGNKRVVYVNSYDAVKHFWITHQSALISRPM

FHTFHSVVSTSQGFTIGTSPWDESCKARRKAAATALNRPATQSYMPILDL

ESFVSIKELFDDCEKGTKDLDPSPYFARFALNTSLTLNYGYRINGDVNSE

LLHEITHVEREISNFRSTSNNWQDYVPLLRLWGAQNS

SAGEFRARRDKYLTDMLNHLKSRIAEGTDKPCITGNILKDPEAKLNE (1)

AEIKSICLTMVSAGLDTVPGNLIM

GIAYLATPHGQEIQAKALEEIYKVYPNGEAWERCLVEEKVPYITALVKEI

LRFWTVIPICLPRVNIKDIPYKGATIPAGTTFFMNAYAADYDESRFAKPF

EFNPERYMDDKEIGTPHYAYGAGSRMCAGSHLANRELYTAFIRLITAFEM

VPPKDKSDEAIMDSIECNAVPTSLTTDPKPFKVGFKTRSESRLREWIAAA

DERTKELR*

 

>CYP504A6 Nectria haematococca

e_gw1.24.52.1 same gene as fgenesh1_pg.scaffold_24000130

Necha1/scaffold_24:331011-332725

72% to CYP504A1 Emericella nidulans phacA gene

85% to 504A5 Fusarium graminearum  FG03741.1 AACM01000162

MSSLVLYLAAVVVLYALIRFLNSTDIPKIKGIPEIPGVPIFGNLIQLGTDHARVAQKWAAKYGPVFQTRLGTK (0)

RVVFVNSYDSVKHFWITHQAALISRPTFHTFHSVVSSSQGFTIGTSPWDDSCKARRKAAATALNRPAVQS

YMPILDLESTTSIRELLEDCDNGNSDLDPSPYFARFALNTSLTLNYGFRIDGDVNSELLHEITHVEREIS

NFRSTSNNWQDYVPLLRLWGAQNSAAGEFRARRDKYLTDMLNDLKQRIANGTDKPCITGNILKDPEAKLNE (1)

AELKSICLTMVSAGLDTVPGNLIMGVAYLATPEGQKIQAKALQAIQEVYPNGEAWEKCLVEEKVPF

ITALVKETLRYWTVIPICLPRVNIRDIPYKDTVIPAGTTFFM (0)

NAYAADYDEARFRSPYEFIPERFLDDKEVGTPHYGYGAGSRMCAGSHLANRELYTAYIRIITAFEILPPKD

KTDFPVMDAIECNANPTSLTTDPKPFKVGFKIRSESKLREWIAEAEERTRGL*

 

>CYP504A7  Aspergillus fumigatus Af293

          GenEMBL XP_748171.1 also

          phenylacetate 2-hydroxylase (predicted)

          85% to 504A1

MAQTIVLAVLAAVYLLIRYLNRTDIPKIKGLPEIPGVPIFGNLLQLGDRHATVAARWAKKYGPVFQVRMG

NKRIVFANSFESVKQLWIKDQSALISRPTFHTFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAV

QSYMPIIDLESTASIKELLKDCRNGAVDINPTAYFQRFALNTSLTLNYGFRIEGNVDDALLREIVDVERG

VSNFRSTSNNWQDYIPLLRIFPKTNREAEEFRVRRDKYLTFLLDMLKERIAKGTDKPCITGNILKDPEAK

LNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEAGQRIQQKAYEEILKVYPNGDAWEKCLVEEKVPYI

TALVKETLRFWTVIPICLPRESTKDIVYNGATIPAGTTFFMNAYAADYDDEHFKMPDKFIPERYLDVSEG

SGTPHYGYGAGSRMCAGSHLANRELYTAYIRLITAFTMHPARDPADQPILDAIECNAIPTALTTEPKPFK

VGFKPRDASKLQQWIAESDERTKEL

 

>CYP504A7  Neosartorya fischeri

           XM_001266358.1, XP_001266359.1

           77% to 504A3, 97% to CYP504A7 Aspergillus fumigatus

           NFIA_040380

           Name revised from CYP504A10

MAQTIVIAVLAAVYFLIRYLNRTDIPKIKGLPEIPGVPVFGNLL

QLGDRHATVAAKWAKKYGPVFQVRMGNKRIVFANSFESVKQLWIKDQSALISRPTFHT

FHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAVQSYMPIIDLEATASIKELLK

DCRNGAVDINPTAYFQRFALNTSLTLNYGFRIEGNVDDALLREIVDVERGVSNFRSTS

NNWQDYIPLLRIFPKTNREAEEFRVRRDKYLTFLLDMLKERIAKGTDKPCITGNILKD

PEAKLNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQRIQQKAYEEIMKVYPN

GDAWEKCLVEEKVPYVTALVKETLRYWTVIPICLPRESTKDIVYNGATIPAGTTFFMN

AYAADYDDDHFKMPDKFIPERYLDVSEGSGTPHYGYGAGSRMCAGSHLANRELYTAYI

RLITAFTMHPARDPADQPILDAIECNAIPTALTTEPKPFKVGFKPRDTSKLQQWIAES

DERTKEL

 

>CYP504A8  Aspergillus oryzae

GenEMBL BAE58419.1

          86% to 504A1, 45% TO 504B6

MSLQTIAIAAIAVAYFIIRYLNRTDVPKIKGIPEIPGVPLFGNLLQLGDQHATVAGKWAKKFGPVFQVRM

GNKRVVFANSFDSVRQLWIKDQAALISRPTFHSFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPA

VQSYMPIIDLEATASIKELLKDSQNGTVDVNPTAYFQRFALNTSLTLNYGFRIEGNVDDELLKEIVDVER

GVSNFRSTSNNWQDYIPLLRIIPKMNREAEEFRVRRDKYLTYLLDILKDRIAKGTDTPCITGNILKDPEA

KLNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQRIQQKAYDEIMKVYPDGDAWEKCLVEEKVPY

VSALVKETLRFWTVIPICLPRVNIKEIEFNGAKIPAGTTFFMNAWAADYDEDHFKMPEKFLPERYLEVSE

GSGTPHYGYGAGSRMCAGSHLANRELYTAYIRLITAFTMHPAQNPADRPILDAIECNEIPTALTTEPKPF

KVGFKPRDAPKLQQWIMESDERTKDL

 

>CYP504A8  Aspergillus flavus

99% to CYP504A8  Aspergillus oryzae

AFL2G_01710

MSLQTIAIAAIAVAYFIIRYLNRTDVPKIKGIPEIPGVPLFGNLLQLGDQHATVAGKWAKKFGPVFQVRMGNKRVVFANS

FDSVRQLWIKDQSALISRPTFHTFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAVQSYMPIIDLEATASIKELL

KDSQNGTVDVNPTAYFQRFALNTSLTLNYGFRIEGNVDDELLKEIVDVERGVSNFRSTSNNWQDYIPLLRIIPKMNREAE

EFRVRRDKYLTYLLDILKDRIAKGTDTPCITGNILKDPEAKLNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQR

IQQKAYDEIMKVYPDGDAWEKCLVEEKVPYVSALVKETLRFWTVIPICLPRVNIKEIEFNGAKIPAGTTFFMNAWAADYD

EDHFKMPEKFLPERYLEVSEGSGTPHYGYGAGSRMCAGSHLANRELYTAYIRLITAFTMHPAQNPADRPILDAIECNAIP

TALTTEPKPFKVGFKPRDAPKLQQWIMESDERTKDL

 

>CYP504A9  Aspergillus terreus 

          XM_001218406.1 XP_001218407.1

          79% to 504A3

          ATEG_09785.1

MALQTLAIAAAAVVYFIIRYFNRTDVPKIKGLPEIPGVPIFGNL

LQLGDKHATVAGAWAKKYGPVFQVRMGNKRVVFANSFDSVRQLWIKDQSALISRPTFH

TFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAVQSYMPIIDLECTASIRELL

KNSQGGTVDLNPAPYFQRFALNTSLTLNYGFRIEGNVDDQLLREIVDVERGVSNFRST

SNNWQDYIPLLRIFPKMNTEAEEYRVRRDKYLTYLLDILKDRIAKGTDKPCITGNILK

DPEAKLNEAEVKSICLTMVSAGLDTVPGNLIMGIAYLASEDGQRIQEKAYDEIMKVYP

NGDAWEKCLVEEKVPYITALVKETLRFWTVIPICLPRESTKEIVYNGAKIPAGTTFFM

NAWAADYDEDHFKMADRFIPERYLEQSEGAGTPHYGYGAGSRMCAGSHLANRELYTAF

IRLITAFTMHPAQNKEDLPVLDAIECNAIPTALTTEPKPFKVGFKPRDAALLEKWIAE

SDERTKEL

 

>CYP504A11  Aspergillus clavatus NRRL 1

           XP_001276373.1, ACLA_003600

           74% to 504A3

  1 MTLQTLAFAV VAAVYLVIRY LNRTDVPKIK GIPEIPGVPL FGNLLQLGDQ HATVAAKWAK

 61 QYGPVFQVRM GNRRIVFANS FDSVRQLWIK DQSALISRPT FHTFHSVVSS SQGFTIGTSP

121 WDESCKRRRK AAATALNRPA VQSYMPIVDL EATASIKELL KNGQNGAVEL NPTAYFQRFA

181 LNTSLTLNYG FRIEGNVDDQ LLREIVDVER GVSNFRSTSN NWQDYIPLLR ILPKTNREAE

241 EFRERRDKYL TYLLDILKDR IAKGIDKPCI TGNILKDPEA KLNEAEVKSI CLTMVSAGLD

301 TVPGNLIMGI AYLASEDGQR IQQKAYDEIM KVYPNGEAWE KCLVEEKVPY VTALVKETLR

361 FWTVIPICLP RESTKDIVYN GATIPAGTTF FMNAYAADYD ADHFKFPEKF IPERYLDVGE

421 GSGTPHYGYG AGSRMCAGSH LANRELFTAY IRLITAFTMH PARDPTDRPI LDAIECNDIP

481 TALTTEPKPF KVSFKPRDAK TLQRWIEESD ERTREL

 

>CYP504A12  Coccidioides immitis RS

           XP_001243121.1 also CIMG_07017.2

           71% to 504A3

  1 MGYQTIAIAV VAVVYFVIKQ LNKTDIPKIK NLPEIPGVPM FGNLIQLGDS HAKKAQDWVK

 61 KYGEVFQVRL GNKRIIFANT FDSVKHFWIT HQSSLISRPT LHTFHTVVSS SQGFTIGTSP

121 WDESCKRRRK AAATALNRPA VQSYMPIIDL ESTVSIKELL NDSKDGTVDV DPNPYFQRFA

181 LNTSLTLNYG IRIDGSIEDE LLKEICHVER VVSNFRSTSN NWQDYIPLLR LWPSRNNEAK

241 EFRERRDRYL SLLLDMLRDR IAKGTDKPCI TGNILKDPEA KLNEAELKSI CLTMVSAGLD

301 TVPGNLIMGI AYLASAHGQE IQERAYQEIM KVYPDGDAWE RCLVEEKVPY ITAFVKEVLR

361 FWTVIPICLP RVSIKDIEYK GATIPAGTTF FMNAYAADYD ENHFKNPYEF RPERYLNVSD

421 GAGTPHYAYG AGSRMCAGSH LANRELYTAF LRLISAFRIV PAKNRADDPI LDCLEAGINK

481 CALTLDPKQF KVGFKVRDRA KLDAWIQGSD ERTKDL

 

>CYP504A13  Phaeosphaeria nodorum SN15

           EAT85524.1

           64% to 504A3

  1 MTIPLYVAGV AVLGFYLYRL LYGTDKPHIK GLPEVPGLPL FGSLVELGTN HAKVAQGWAK

 61 KYGPVFQVRM GNRRIVFANS FDSVRHLWIT HQSALISRPT LHTFHTVVSS SQGFTIGTSP

121 WDESLKNRRK AAGTALNRPA VQSYMPLIDL ESTVSIRELL NDSKGGELDI DPRAYWQRYA

181 LNTSLTLNYG FRIDGDKDAP LLKEICDVER GVGNFRSTSN NWQDYVPLLR LFPSQSNEAK

241 KFRDRRDVYM NKLLDTLLEK IEKGTDKPCI TGNIIKDPDA KLNRAELKSI CLTMVSAGLD

301 TVPGNLIMGI AQLATPSGQS IQRRAHAEIL KVYPNNDAWH KCLEEEKIPY ITALYKEILR

361 YWTVIPISLP RQSIKDIEYN GVTFPAGTTF YMNAYAADYD DTHFSDPYTF NPERYLDVPD

421 GTGTPHYGYG AGSRMCIGSH LANRELYTAF VRLISAFEFT EPVDERDKPI MDCLEANAIP

481 TSLTLEPKYF KVGFKPRDRA LLEKWLGESE ERTRELKGMG K

 

>CYP504A14 partial Gibberella moniliformis cDNA DR661198 87% to 504A3

MSDQVIYLAIIIGLYMLYK  LANSTDQPKIKGIPEIPGVPLFGNLIQLGTDHARVA

QKWAKKYGPVFQTRLGTKRVIYVNSYEAVKHFWITHQSALISRPMFHTFHSVVSSSQGFT

IGTSPWDESCKNRRKAAATALNRPAVQSYMPILDLESLVSIEELLNDCKDGSKDLDPSPY

FARFALNTSLTLNYGFRIDGDVNSELLHEITYVEREISNFRSTSNNWQDYVPLLRLWGAQ

NSSAEEFRVRRDKYLSDLLANLKSRIDNGTDKPCITGNILKDPTAKLNDAEIQSICLTMV

SAGLDTV

 

>CYP504A15 Aspergillus niger

fgenesh1_pm.C_scaffold_7000252|Aspni1

88% to CYP504A8

MAVQTILIAAAAVIYFLVRYMNRTDVPKIKGIPEIPGVPLFGNLLQLGTQHAVVARKWAKSFGPVFQVRMGNKRVVFANS

FDSVRQLWIKDQSALISRPTFHTFHSVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAVQSYMPIIDLECTASIKELF

KDSQNGARDINPKAYFQRFALNTSLTLNYGFRIEGNVNDELLHEIVNVERGVANFRSTSNNWQDYIPLLRIFPKQNREAQ

EFRERRDKYLSYLLEILKDRIAKGTDKPCITGNILKDPEAKLNEAEIKSICVTMVSAGLDTVPGNLVMGIAYLASEDGQR

IQQKAYDEIMKVYPNGDAWEKCLVEEKVPYVSALVRETLRFWTVIPICLPRESTKDIEYNGATIPAGTTFFMNAWAADYD

EDHFKMPEKFIPERYLDVGEGSGTPHYGYGAGSRMCAGSHLANRELFTAYVRLITAFTMHPSRDLADRPILDALECNEIP

TGLTTEPKPFKVGFTPRDPTKLQQWIAESDERTKDL*

 

>CYP504A16 Mgr075 Mycosphaerella graminicola

67% to CYP504A12   Coccidioides immitis

MAQSPEPQSLTGLLFTPQVFPFFILAVIYAAIKIIDRTDIPKIKGLPEVPGVPIFGNLRQLGDNHAVNAMKLAKKYGPVFQVRLGNRRIVFANTFDSVRNLWITNQSALISRPKLHTFHTVVSSSEGFTIGTSPWDESCKKRRKAAATALNRPAVQSYMPIIDYESIKSIKEMLQDSQQGQIDIDPNPYFQRYALSTSLTLNYGIKIEGNIDDGMLQEIVHVEREVSNFRSTATNWADYIPMLRLLPGGSDKPLEYKKRRAAYMHKLLGMLKSRIADGTDAPCISGNVLKDPEAKLTDAEVMSIGLTMVSAGLDTVPGNLIMCIAYLSSPHGQEIQSRALAEIQKVYPNNDSWKRCLHEEKCSYITALVKETLRFWSVIPVCLPRTSTKPIQWEGVTIPAGTSFYMNAYAAHYDDSHFSSPFDFNPDRYLADDSIDDAAASRGTPHYGYGAGSRMCIGSHLANRELYTAFLRMITAFEILPPKRESDRPILDAMGCNKLPNGLTMDPKGFKIGLRVRDKGALERWMREGEERTRSL

 

>CYP504A17 Yarrowia lipolytica

49% to CYP504A6   Nectria haematococca

CAG77765.1

MLLQLLSSILLAVLTFIILDWLFEIDTETPHIAGLPAKRGYPIVGAVFQLGKSHPLTYRKWSEELGPVYQVRLGNKRVVV

ANSYASVRELWITNMRANISRPESYTFHKLLSSKDGPTIGTTPWGVSARRKRRAASTALNKPSVAAYTPIINLESYKCIR

RFLRDCGGIEGEVDVSVGLATYSLNTSLTLNYGIMTADFDSTYLAEVMDIEERILRLRSTMHNTQDYIRYLRVFSRGSTV

EAAECRERRAQYMTKFLKKLKQEIDDGTDMPCIAGNILKDKESKITDADLRSICLTMVSAGFDTVPANLISCFGHLSHSY

GQTIQKRALEAIDRAYPDGDAWEMCLMDDSKVHYIVALCEETVRYGSVVPMALPRRTINDIHYQGAVIPAGTILFMNAFA

ANFDATRFHDPFKFDPERYLDLDGRLKTELGPAHFGFGAGSRMCCGIHLARKELYVALVRFILAFRILPPVDPKDMMVND

PVEIFAQPENMTTKPPPFKVRLQVRDAEKLDRWLKETKEGLGETWSVGHD

 

>CYP504A18  Uncinocarpus reesii

94% to CYP504A12    Coccidioides immitis

UREG_05848.1

MGYQTIAIAAVAFVYFVIKQLNKTDIPKIKNLPEIPGVPIFGNLIQLGDSHAKRAQEWVKQYGEVFQVRLGNKRIIFANT

FDSVKHFWITHQSALISRPTLHTFHTVVSSSQGFTIGTSPWDESCKRRRKAAATALNRPAVQSYMPIIDLESTASIKELL

HDSKDGTVDIDPNPYFQRFALNTSLTLNYGIRIDGSIEDELLKEICHVERVVSNFRSTSNNWQDYIPLLRLWPSRNNEAK

EFRERRDKYLSLLLDMLRDRMAKGTDKPCITGNILKDPEAKLNEAELKSICLTMVSAGLDTVPGNLIMGIAYLSTAHGQE

IQDRAYKEIMQVYPNGDAWERCLVEEKVPYITALVKEVLRFWTVIPICLPRVSVKDIEWKGATIPAGSTFFMNAYAADYD

ENHFKNPYEFRPERYLNVSDGAGTPHYAYGAGSRMCAGSHLANRELYTAFLRLISAFRIVPAKNRADDPIMDCLEAGINK

CALTLDPKQFKVGFRVRDRAKLDSCIQGSDERTKDL

 

>CYP504A19 Histoplasma capsulatum G217B

80% to CYP504A12 Coccidioides immitis

ABBT01000061.1 Ajellomyces capsulatus G217B

HCB01876.1

154004  MGLQTAVVAVIAVGFLLIRYLNRTDIPKISKLTEIPGIPIFGSLIQLGEEHARVAAGWVK  154183

154184  KYGPVFQVRLGNK (0)  154222

154380  RIVFANTFDSVKYFWITHQSALISRPTLHTFHTVVSSSQGFTIGTSPWDESCKRRRKAAA  154559

154560  TALNRPAVQSYMPIIDLESNVSIRELFHDSKGGTVDIDPNAYFQRFALNTSLTLNYGIRI  154739

154740  DGNIDNELLKEICHVERIVSNFRSTSNNWQDYVPLLRLWPSRNSEAKEFRIRRDKYLTLL  154919

154920  LDMLRERIKNGTDKPCITGNILKDPDAKLNE (1)

AEIKSICLT

MVSAGLDTVPGNIIMGIAYLASDHGQQIQARAYDEIMKVYPNGDAWEKCLVEEKVPYITALVKEVLRFFTVIPICLPRVS

IKDIPYEGAVIPAGTTFFMNAYAADYDATHFKNPHLFEPERYLSATDGSGTPHYAYGAGSRMCAGSHLANRELYTAFIRL

ITAFRMMPARDPRDHPILDALDCNATKTSLTLDPMPFKVWFRVRDAESLERWMRESEERTREL* 155999

 

>CYP504A20 Fusarium oxysporum

85% to CYP504A5 Fusarium graminearum

FOXG_09779

MSDQVIYLAIIIGLYVLYKLANSTDQPKIKGIPEIPGVPLFGNLIQLGTDHARVAQKWAKKYGPVFQTRLGTKRVIYVNS

YEAVKHFWITHQSALISRPMFHTFHSVVSSSQGFTIGTSPWDESCKNRRKAAATALNRPAVQSYMPILDLESL VSIKELL

DDCKDGS KDLDPSPYFARFALNTSLTL NYGFRIDGDVNSE LLHEITYVEREISNFRSTSNNWQDYVPLLRLWGAQNSSAE

EFRVRRDKYLSDLLANLKSRIDNGTDKPCITGNILKDPTAKLNDAEIKSICLTMVSAGLDTVPGNVIMGIAYLATEHGQK

IQAKALKEIYKVYPNGEAWERCLVEEKVPYVTALVKEILRFWTVIPICLPRESIKDIPYQGAVIPAGTKFFMNAYAADYD

ETRFANPYEFDPERYIDDKEVGTPHYAYGAGSRMCAGSHLANRELYTAFIRLITAFEILPPKDNKDFPVMDCIECNANPT

SLTTDPEPFKVGFKPRDEAKAREWIAAGEERTQDIR*

 

>CYP504A20P Fusarium verticillioides

96% to CYP504A20 Fusarium oxysporum = ortholog with 3 frameshifts

FVEG_08703

MSDQVIYLAIIIGLYMLYKLANSTDQPKIKGIPEIPGVPLFGNLIQLGTD

HARVAQKWAKKYGPVFQTRLGTKRVIYVNSYEAVKHFWITHQSARISRST

FHTFQSVVSSSQVFTIGTSPLDESCKNRRKAAATALNRPAVQSYMPILDLESL

VSIEELLNDCKDGS &

KDLDSS &

PYFVRIALNTSLTL &

NYGFRIDGDVNSE

LLHEITYVEREISNFRSTSNNWQDYVPLLRLWGAQNSSAEEFRVRRD

KYLSDLLANLKSRIDNGTDKPCITGNILKDPTAKLNDAEIKSICLTMVS

AGLDTVPGNVIMGIAYLATEHGQKIQAKALKEIYKVYPNGEAWERCLVEEK

VPYVTALVKEILRFWTVIPICLPRESIKDIPYQGTVIPAGTKFFMNAYAA

DYDESRFANPYEFNPERYIDDKEVGTPHYAYGAGSRMCAGSHLANRELYT

AFIRLITAFEILPPKDKKDFPVMDCIECNANPTSLTTDPKPFKVGFKPRD

EAKAREWIAAGEERTQDIR*

 

>504B1 Aspergillus nidulans AACD01000019.1 ANcontig1.19    68186 to 69958

MAIAEALLFV NNRAVEHPLQ FLTAVAFAVP LLYVVINEFI RASARIPGFK GPRGLPLIGN

LAQIRKDAAE QYRIWSKIYG PVYQIQLGNI PVLVVNSAAA AKVLFGQNAQ ALSPRPEFYT

FHKIVSNTAG TTIGTSPYSE SLKRRRKGAA SALNRPSVES YVHHLDVESK AFVAELFKYG

NGGKTPVDPM AMIQRLSLSL ALTLNWGVRV ASQEEELFDE ITEVEDKISK FRSTTGNLQD

YIPILRLNPF SSNSHKAKEM RDRRDKYLSS LNRDLDDRME KGTHKPCIQA NVILDKEAKL

NSEELTSISL TMLSGGLDTV TTLVAWSISL LAQRPDIQDK AAKAIQEMYS EGQPLCDPAD

DQKCAYVAAL VRECLRYYTV LRLALPRTSI RDITYDGKVI PKGTVFFLNA WACNMDPEVW

SDPDEFRPER WFEQPDAPMF TYGMGYRMCA GSLLANRELY LVFIRTLNSF RIEPTEEKIE

WHPLKGNSDP TSLVAIPKKY KVRF

VPKNEV SLVEALS

 

>CYP504B1 Aspergillus nidulans AN1397.1 63% to 504B2 100% to 504B1

MAIAEALLFVNNRAVEHPLQFLTAVAFAVPLLYVVINEFIRASARIPGFK

GPRGLPLIGNLAQIRKDAAEQYRIWSKIYGPVYQIQLGNIPVLVVNSAAA

AKVLFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSESLKRRRKGAA

SALNRPSVESYVHHLDVESKAFVAELFKYGNGGKTPVDPMAMIQRLSLSL

ALTLNWGVRVASQEEELFDEITEVEDKISKFRSTTGNLQDYIPILRLNPF

SSNSHKAKEMRDRRDKYLSSLNRDLDDRMEKGTHKPCIQANVILDKEAKL

NSEELTSISLTMLSGGLDTVTTLVAWSISLLAQRPDIQDKAAKAIQEMYS

EGQPLCDPADDQKCAYVAALVRECLRYYTVLRLALPRTSIRDITYDGKVI

PKGTVFFLNAWACNMDPEVWSDPDEFRPERWFEQPDAPMFTYGMGYRMCA

GSLLANRELYLVFIRTLNSFRIEPTEEKIEWHPLKGNSDPTSLVAIPKKY

KVRF

DLISELIREYDTR*

 

>CYP504B2 Magnaporthe grisea MG03352.4  63% to 504B1 Aspergillus nidulans

AACU01000699 cont2.663

MAITSLVAWAAGPATSFSCVRICTLAVTSIVLYFVINEIIRAKSRIKGLA

GPRGLPLIGVLWDIRTNAAEQYRRWAKRYGDVYQVQLGNVTVVVVNSAES

ARTLFGHNSQALSSRPEFYTFHKVLSDTAGTTIGTSPYSESLKRRRKAAA

SALNRPSVASYVGILDLETRDFVLEMVAYGKHGTKAIDPMPMIQRLSLSL

ALTLNWGSRVASQHDGLFSEITHVEEEISKFRSTTGNLQDYVPLLRINPF

NGHSARAREMRNRRDVYLKHLDDDLRRRMAAGTQRPCIQANIILDDNDIK

ADSAQRLSKAEMTSISLTMLSGGLDTITTNVAWFIALLVQRPDIQEKAHL

EIRKHLIDQVQPDRSDPLGLCDAEDDQGCRYVAALVRESLRYYTVLRLAL

PRVSVREVVHEGVVLPRGTVFFLNAWACNMDAKVWTDPEVFRPERWLEQP

DAPLFTYGVGYRMCAGSMLANRELYLLYMRLIAHFKIDPVPAKDGCDGID

VVDCHPVSGNCDPTSLVAIPRPYKVLFVPRDLDFIGMSVA*

 

>CYP504B3 Fusarium graminearum FG06447.1 AACM01000259 FGcontig1.259_scaffold4

MTIANILLSLNDKVQKNKGQSILITLFIAPLVYILVNELVRHNARISNLD

GPWGLPLIGNIRDIKVNASEKYREWAKKYGPVYQIQLGNIPIVVVNSAAA

ARHIFGANAQALSSRPEFYTFHKVNHTTPNTCQGXXXXXXXXXXXXXXAASALNRPSVATYVDH

LDVETRDFIKELIEYGNNGKSPVDPMPMIQRLSLSLALTLNWGVRLKSQK

DNLFAEITHVEEEISRFRSTTGNLQDYIPLLRLNPFNFHSSKAREMRNRR

DKYLTALNDGLDERIKNGTSKPCIQANVIMDKEXXXXXXXAKLNKDELTSISLTMLS

GGLDTVTTLVAWFVAYLSQHPEIQDKAVSEIGKFFSQKEPXXXXXXXMCDANDDQKC

EYVVALVKESLRYYTVLRLALPRASIRDIIYEGVKIPKGTVVFLNAWACN

MDEQVWSDPQEFRPERWLEQPEAPLFTYGVGYRMCAGSLLANRELYLLYM

RLLNSVKIEQXXXXXXXXXFDNVDCHPVRGNLDPTSLVAMPRRYKASFTPRSPDDLKKA

LVVSV*

 

>CYP504B4 Nectria haematococca

e_gw1.24.45.1

Necha1/scaffold_24:269356-271018

79% to 504B3 F. gram.

43% to e_gw1.24.52.1,  66% to 504B1 Aspergillus nidulans

This gene model seems correct

MTIANLLVSLNDKVQENKGQSVLLTLVIAPLIYLFINELVRHNARISNLQGPSGLPLIGNIWDIHINAAE

KYREWSRKYGKVYQIQLGNIPIVVVNSASAARTIFGANAQALSSRPEFYTFHK (0)

VLSDTAGTTIGTSPYSD

SLKRRRKGAASALNRPSVATYVDHLDIETRDFIKELCNYGEAGKIPVDPMPMIQRLSLSLALTLNWGIRL

KSQEDDLFNEITHVEEEISRFRSTTGNLQDYIPLLRLNPLNLSSSKARSMRDRRDKYLGDLNRGLDERIA

NGTYKPCIQANVIMDKEAKLNKDELTSISLTMLSGGLDTITTQVAWSIAYFAQHPEIQEKAKAEIEKFYA

TNQPMCDEEDDQKCQYIVALVRESLRYFTVLRLALPRASIRDVVYNGVTIPKGTVVFLNAWACNM (1)

DEQVWTDPEVFRPERWLEQPDAPLFTYGVGYRMCAGSLLANRELYLLYMRLLNSFKIEKHDDIDCDPITGNLDPT

SLVAMPRRYKAVFTPRDPKALKAALAQQVE*

 

>CYP504B5   Aspergillus fumigatus Af293

           GenEMBL XP_752247.1 also EAL90209.1

           phenylacetate hydroxylase predicted

           68% to 504B4

MAIAAAVSSLQQNVLRHPLQSLAITALVVPIIYIIINEFIRASARVPGFKGPRGLPLIGNLAQIRKNAAE

QYRIWSKTYGPVYQIQLGNIPIIVVNSAAAAKILFGQNAQALSSRPEFYTFHRIVSNTAGTTIGTSPYSE

SLKRRRKGAASALNRPSVESYVSHLDVETKAFVAELLKYGNGGKTPVDPMAMIQRLSLSLSLTLNWGVRV

ASQEEDLFNEITHVEEEISKFRSTTGNLQDYIPLLRLNPFSTNSRKAKEMRDRRDRYLNALNRDLDDRME

KGIHKPCIQANVILDKEAKLNKEELISISLTMLSGGLDTITTLVAWSFCLLSRRPDIQEKAAQAIQDMFS

QDEPLCDAQDDQKCAYVAALVRECLRYYTVLRLALPRTSIKDITYDGKVIPKGTVFFLNSWACNMDPEVW

NDPEEFRPERWFEQPDAPMLTYGIGYRMCAGSLLANRELYLVFMRTLNSFHIEPHDDVDWHPINGNSDPT

SLVAIPKKYKVRFVPKNEQALTRALA

 

>CYP504B5 Neosartorya fischeri

98% to CYP504B5 Aspergillus fumigatus = ortholog

NFIA_016920

MAIAAAVSSLQQNVLRHPLQSLAVTALVVPIIYIVINEFIRASARVPGFKGPRGLPLIGNLAQIRKNAAEQYRIWSKTYG

PVYQIQLGNIPVIVVNSAAAAKILFGQNAQAVSSRPEFYTFHRIVSNTAGTTIGTSPYSESLKRRRKGAASALNRPSVES

YVSHLDVETKAFVAELLKYGNGGKTPVDPMAMIQRLSLSLSLTLNWGVRVASQEEDLFDEITHVEEEISKFRSTTGNLQD

YIPLLRLNPFSTNSRKAKEMRDRRDRYLNALNRDLDDRMEKGIHKPCIQANVILDKEAKLNKEELISISLTMLSGGLDTI

TTLVAWSFCLLSRRPDIQKKAAQAIQDMFSQDEPLCDAQDDQKCAYVAALVRECLRYYTVLRLALPRTSIKDITYDGKVI

PKGTVFFLNSWACNMDPEVWSDPEEFRPERWFEQPDAPMLTYGIGYRMCAGSLLANRELYLVFMRTLNSFYIEPHDDVDW

HPINGNSDPTSLVAIPKKYKVRFVPKNEQALTQALA*

 

>CYP504B6  Aspergillus oryzae

          GenEMBL BAE56546.1

          82% to 504B1

MAITATLFSIQKSLIHTPLQSIAIAALFTSCLYFIVNEFIRAFSRVPGFKGPRGLPLIGNIAQIRKNAAE

QYRIWSKTYGPVYQIQLGNIPVIVVNSAVSAKAIFGQNAQALSSRPEFYTFHK

 

IVSNTAGTTIGTSPYSD

SLKRRRKGAASALNRPSVESYVSHLDAESKAFVAELLKYGEGGNVPVDPMAMIQRLSLSLALTLNWGVRV

VSQEEDLFNEITHVEEEISKFRSTTGNLQDYIPLLRLNPFNTNSKKAKEMRDRRDKYLNGLNRDLDDRME

KGIHKPCIQANVILDQEAKLNSEELTSISLTMLSGGLDTVTTLVAWSIGLLAKRPDIQDKAAEAILNMYG

PNQPMCDAADDQRCAYVAALVRECLRYFTVLRLALPRTSIRDITYGGNIIPKGTVFFLNSWACNMDPDTW

SDPEEFRPERWLEQPDAPLFTYGLGYRMCAGSLLANRELYLIFMRTINSFRIEPYDEIDWHPVHGNADPT

SLVAIPKKYKV

RFIPRDNKVLEDVLGSAFSLKRVI

 

>CYP504B6  Aspergillus flavus

100% to CYP504B6  Aspergillus oryzae

AFL2G_01532

MAITATLFSIQKSLIHTPLQSIAIAALFTSCLYFIVNEFIRAFSRVPGFKGPRGLPLIGNIAQIRKNAAEQYRIWSKTYG

PVYQIQLGNIPVIVVNSAVSAKAIFGQNAQALSSRPEFYTFHK (0)

IVSNTAGTTIGTSPYSDS

LKRRRKGAASALNRPSVESYVSHLDAESKAFVAELLKYGEGGNVPVDPMAMIQRLSLSLALTLNWGVRVVSQEEDLFNEI

THVEEEISKFRSTTGNLQDYIPLLRLNPFNTNSKKAKEMRDRRDKYLNGLNRDLDDRMEKGIHKPCIQANVILDQEAKLN

SEELTSISLTMLSGGLDTVTTLVAWSIGLLAKRPDIQDKAAEAILNMYGPNQPMCDAADDQRCAYVAALVRECLRYFTVL

RLALPRTSIRDITYGGNIIPKGTVFFLNSWACNMDPDTWSDPEEFRPERWLEQPDAPLFTYGLGYRMCAGSLLANRELYL

IFMRTINSFRIEPYDEIDWHPVHGNADPTSLVAIPKKYKV

RFIPRDNKVLEDVLGSAFSLKRVI*

 

>CYP504B7 Aspergillus niger

estExt_fgenesh1_pm.C_30542|Aspni1

85% to CYP504B5

MAIAAVLSFLQQSILESPLQSLAATAFIVPVLYIIINEFIRASARVSGLKGPRGLPLIGNLAQIRKNAAEQYRIWSRTHG

PVYQIQLGNIPVVVVNSAAAAKVLFGHNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSESLKRRRKGAASALNRPSVES

YISHLDVESRSFVDELYRYGNGGETPVDPMPMIQRLSLSLSLTLNWGVRVASQEEDLFAEITHVEEEISKFRSTTGNLQD

YIPLLRLNPFNSNSKVAKEMRDRRDRYLTALNRDLDDRMKKGIHKPCIQANVILDKEAKLNSEELISISLTMLSGGLDTV

TTLVAWSIGLLSQRPDIQEKAAKAIRDMYGHDQPLCDPMDDQRCAYVAALVRECLRYFTVLRLALPRTSIRDVVYNGTVI

PKGTVFFLNSWACNMDPEVWSDPEEFRPERWLEQPDAPLFTYGMGYRMCAGSLLANRELYLVFMRTLNGFRIEPLGETDW

HPVRGNSDPTSLVAIPKKYKVRFVPRDEKALAKALGR*

 

>CYP504B8 Uncinocarpus reesii

77% to CYP504B5

UREG_05702.1

MALVSIVSELNSSAITHPFRALAVLALLIPGVYVIFNEFVRAAARVPGLNGPKGFPLIGNLSQIRVNAAEQYRKWSKQYG

PVYQIQLGNIPVIVVNSAAAAKVLFGQNAQALSSRPEFYTFHKVVSNTAGTTIGTSPFSESLKRRRKGAASALNRPSVQT

YVPHLDLESKDFVAELMKYGKAGRVAVDPMPMIQRLSLSLALTLNWGIRMASQEEDLFAEITHVEDEISRFRSTTGNLQD

YIPLLRLNPFSVGSKKAAEMRDRRDKYLKYLNSGLEDRMAKNEHKPCIQANVMLDKEARLNNIELTSISLTMLSGGLDTI

TTLVAWSIALLAQRPDIQDKAAAAIKEYHSVDQPLCDAMDDQRCEYIVALVRECLRYYTVLRLALPRTSIKDITYQGKVI

PKGSVFFLNAWACNMDPDVWSDPEVFRPERWLEQPDAPMFTYGVGYRMCAGSLLANRELYLVFLRTLNCFRIEPDGKVEW

DPISGNSDPTSLVAIPKKYKVKFVPKKEKLLTEALENFVPVSA

 

>CYP504B9 Coccidioides immitis

91% to CYP504B8

CIMG_07204.2

MALSSFISALNSQAIAHPFHALGVLALLIPGIYVIANEFVRAAARVPGFKGPKGFPLIGNLAQIRVNAAEQYRRWSKKYG

PVYQIQLGNIPVIVVNSAAAAKVLFGQNAQALSSRPEFYTFHKVVSNTAGTTIGTSPFSESLKRRRKGAASALNRPSVQT

YVSHLDLESKDFVAELMKYGKAGRAAVDPMPMIQRLSLSLALTLNWGVRMASQEEELFAEITHVEDEISRFRSTTGNLQD

YIPLLRLNPFSVGSKKAAEMRDRRDKYLNYLNRGLEERMAKNEHKPCIQANVMLDKEARLNNVELTSISLTMLSGGLDTI

TTLVAWSIGMLAERPDIQDKAAAAIKQYHSIDKPLCDPTDDQSCEYIVALVRECLRYYTVLRLALPRTSIKDITYQGKVI

PKGSVFFLNAWACNMDPEVWSDPEIFRPERWLEQPDAPMFTYGVGYRMCAGSLLANRELYLVFLRMLNCFRIEPEGRIEW

DPIKGNSDPTSLVAIPKKYSVRFVPKKEKLLTEALENFIPLAA

 

>CYP504B10 Fusarium oxysporum

87% to CYP504B4 Nectria haematococca

FOXG_07489

MTIANLLVSLNNQVHENKGQSLLVTLLIGPLIYIFINELIRHNARISNLKGPSGLPLIGNIWDIHINAAEKYRAWAKKYG

PVYQIQLGNIPIVVVNSAAAARSIFGANAQALSSRPEFYTFHK VLSDTAGTTIGTSPYSDSLKRRRKGAASALNRPSVAT

YVDHLDVETRDFVKELFEYGKGGSIPVDPMPMIQRLSLSLALTLNWGIRLKSQKDDLFSEITHVEEEISRFRSTTGNLQD

YIPLLRLNPFNLHSTKAREMRNRRDKYLTALNNGLDERIANGTYKPCIQANVIMDKEAKLNKDELTSISLTMLSGGLDTV

TTQVAWFVAYLSQHPEIQEKAVSEIRKFYSEKQPMCDEQDDQKCEYIVALVKESLRYYTVLRLALPRASIRDIVYEGVTI

PKGTVVFLNAWACNMDDQVWSDPEVFRPERWFEQPDAPLFTYGVGYRMCAGSLLANRELYLVYMRLLNSLKIEKSDEVDC

HPITGSLDPTSLVALPRRYKAIFKPRNPEALQKALAVEN

 

>CYP504B10 Fusarium verticillioides

98% to CYP504B10 Fusarium oxysporum = ortholog

FVEG_04417

MTIANLLVSLNNQVHENKGQSLLVTLLIGPLIYILINELIRHNARISNLKGPSGLPLIGNIWDIHI

NAAEKYRAWAKKYGPVYQIQLGNIPIVVVNSAAA

ARSIFGANAQALSSRPEFYTFHKVLSDTAGTTIGTSPYSDSLK

RRRKGAASALNRPSVATYVDHLDIETRDFVRELYEYGKGGNIPVDPMPMIQRLSLSL

ALTLNWGIRLKSQKDDLFSE

ITHVEEEISRFRSTTGNLQDYIPLLRLNPFNMHSAKAKEMRNRRDKYLTDLNNGL

DERIANGTYKPCIQANVIMDKEAKLNKDELTSISLTMLSGGLDTV

TTQVAWFVAYLSQHPEIQEKAVSEIRKFYSEKQPMCDEQDDQKCEYIVALVKESLR

YYTVLRLALPRASIRDIVYEGVTIPKGTVVFLNAWACNMDDQVW

SDPEVFRPERWFEQPDAPLFTYGVGYRMCAGSLLANRELYLVYMRLLNSLKIEKSDE

VDCHPITGSLDPTSLVALPRRYKAIFKPRNPEALQKALAVEN*

 

>CYP504B11 Fusarium oxysporum

63% to 504B9 Coccidioides immitis

FOXG_10783 revised with gc boundary at EXXR motif

No ortholog found in Fusarium verticillioides

MLIAELFKALSNEAVTRPYQTAVTSVLVSFLAYIIVNEYVARSARLAAFDGPKRLPIVGNLHQLRSNAAEKYRKWAKTYG

PVYQIQLGNVPILLVNSASAARTVFGTNSSATASRPEFYTFHKLVSNTAGTTIGTAPYSESLKRRRKGAASALNRPAVET

YIPHLDLETKDFIAEAYKFGKAGKTEVDPLPLVQRLSLSLALTLNWGTRMPSSDSDLLREITHVEVEISRFRSTTDNLQD

YIPLLRLNPFSFGHKKAAEYRNRRDAYLGMLNRDLEERVSKGTHKPCIQANIMLDREAKLNDVEMMSISLTMLSGGFETF

STVTTWAIAYLATHPEIQDKAYKAITDLHGSKSPLCDPNDDQKCDYVRALVRESLR (2)

YFTVLRLSLPRATNKDFYYEGKFIPKGTVV

FLNSWACNMDDELWEDPEIFRPERWLEHPDHPMFTFGVGYRMCAGSLLANRELYLTFMRMIASFELSTRDEIDTNPLTGV

EDLTSLVSTPRKYRVTFKPRNDAVLKAAIAQGFDE*

 

>CYP504B12 Aspergillus clavatus

89% to CYP504B5

ACLA_026760

MAITAALSFVQYSILHYPLQSFAAAVLIVPFIYVIANEFIRASVRVPGFEGPRGLPLIGNLAQIRKNAAEQYRIWSKTYG

PVYQIQLGNIPVIVVNSAAAAKVLFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSESLKRRRKGAASALNRPSVDS

YVSHLDVESKAFVAELYKYGHSGKTPVDPMAMIQRLSLSLSLTLNWGVRVASQEEDLFNEITHVEEEISKFRSTTGNLQD

YIPLLRLNPFSSNSRKAKEMRQRRDKYLGALNRDLDDRMEKGIHKPCIQANVILDKEAKLNSEELISISLTMLSGGLDTV

TTLVAWSICLLSRRPDIQEKAARAIHIMFTEDKPLCDSGDDQKCAYVAALVRECLRYYTVLRLALPRTSIRDITYDGKVI

PKGTVFFLNAWACNMDPEIWSDPEEFRPERWFEQPDAPMFTYGMGYRMCAGSLLANRELYLVFMRTLNSFRIEPHDDVDW

HPISGNSDPTSLVAIPKKYKVRFIPKNAKALARDLF*

 

>CYP504B13 Aspergillus terreus

85% to CYP504B5

ATEG_08499.1

MAIAAALASLQRSAVDHPLQSLAVAALLVPIIYVIINEFIRASARVPGFKGPRGLPLIGNLAQIRTNAAEQYRLWSKTYG

PVYQIQLGNIPVIVVNSAAAAKVLFGQNAQALSSRPEFYTFHKIVSNTAGTTIGTSPYSDSLKRRRKGAASALNRPSVES

YVSHLDVESKAFVAELLKYGDAGKTPVDPMAMIQRLSLSLALTLNWGVRVASQEEELFDEITHVEEEISKFRSTTGNLQD

YVPLLRLNPFSSNSQKAKEMRNRRDRYLNGLNRDLDDRMEKGIHKPCIQANVILDKEAKLNAEELTSISLTMLSGGLDTV

TTLVAWSIGMLAKRPDIQEKAFKAIGEFYGEDEPMCDAHDDQKCAYVAALVRECLRFFTVLRLALPRTSIRDITYNGAVI

PKGTVFFLNAWACNMDPEVWHDPEEFRPERWLEQPDAPMFTYGMGYRMCAGSLLANRELYLVFMRTLNSFRIESDEDINW

HPVKGNSDPTSLVAIPQKYKVRFVPRSEKVLAQVVA*

 

>CYP504B14 Mycosphaerella fijiensis

68% to CYP504B8   Uncinocarpus reesii

fgenesh1_pg.C_scaffold_22000145

MIGHTFDLLQRQALEHTLRTAIVLLICLPITYFLINEVIRYRARLPAFPGPRGLPVIGNIADIRYNAAEKYRQWSRRYGP

VYQIQLGNIPVIVVNNAQAAKTIFGNNSQALSSRPVFYTFHKVLSNTAGTTIGTSPFSDSLKRRRKGAASALNKPSVQTY

IPHLDIETLSFVREAFKYGDAGKTALDPMPLIQRLSLSLSLTLNWGTRMGDSENALFHEITEVEEAISRFRSTTGNLQDY

IPLLRLNPFSFGTRKAAEMRARRDVYLKKLNADLDSRMEKGTHKPCIQANVILDREASLTAEELTSISLTMLSGGLDTIT

TLLQWSIALLSQRPDIQTKAITEIRNLHPTLLCDENDDMKCEYIVALVKECLRYYTVLRLALPRSTVRDVTFENKLIPSG

TTMFLNAWACNMDEEIWGSDAEVFRPERWLEKPDAPLFTYGMGYRMCAGSLLANRELYLVLMRMLSCFEILPYGEVDVHP

VRGSCDPKSLVAMPERYQVRFVPRDGEGLRRVLEEKEGMGVFE*

 

>CYP504C1 Ustilago maydis 521, UM01424.1 predicted mRNA.

XM_399039

MRFELSSESLATVPLFVWLSATLVVGHILYTAVKRPKINLPGPR

GWPYVGNLFQLGEDAAQTYYKWSKRYGPVYKVQLGEREVIIINSAQASKELLTDLGSI

YISRPLFYTFHTVVSSTAGFTIGTSPWDESCKRKRRAAATALNRVAVQSYVPIIDRET

FSLVEDLYSAKGKAIAPFAYFQRLALNVSLSVNYGSRLDKISDELLQEIVEVETHVAN

FRSVSNSAADYLPIVRYVQMLVGGNNSFAAQIRARRDKYMRRLLEDLKARVKAGTDIP

CITGNIIRDPTAKLTDLELSSICLSMVSAGLDTLANTFIWSMGFLAKHPEIQEKAYSE

LSKVYQGQVPNSDSEQVEYITALHKECSRYFSVLKLALPKATISDSSYKGVNIPAGTT

VFLNAFAIHQDAERYGDVENFRPERFLEPGEENKQSHYSFGAGRRMCAGVHLANRELY

IAFCKVIHFFHIQLSDVEEEKHFDINPKTGCANPKGLSSTPKPFKVRFVPRHPAALEQ

WIESEREKTDLMASAA

 

>CYP504D1 Ustilago maydis 521, UM05074.1 predicted mRNA.

XM_402689

MNTTKLLGTGALSPSFVFDHDSGNAIFGLSSSTLVVLVAMIAVS

TLTLKSVLPGDRSINLPGPRGWPIVGSWFDLGNNWAEYFRQAAKEYGDVFKVHIGNRT

VVVVNSPKAAHILFNEHGSSLISRPWFYTFHGVLSKSSAFTIGTSAWSDSTKNKRKAA

ATALNRPAVQSYMPIIVEESLDAVRRILNDGNAGKNGIVPYSYFQRLALNTSFQVNYG

FRMGERDDGLFDEISEVIAKVASVRAVTGSLQDYVPLMRYLPANAKSKAAASYGLRRK

KFMSKLYEELEQRVNQGKDESCITGNILKDTESRKKLSRLEIDSICLSMVSAGLDTFA

NTMIWTIGFLAKHPEIQRKAQAELLAHYPNRELPHVDSEDLVYIHAMAKEASRLFNVF

RICLPRTNVSDVTYNNAVIPAGTTFFLNSWACNVDAEKFADPFEFKPERFMDKSASNA

HVENKMGGVETYAFGMGRRMCPGVFLALREIYTTLVFLTHFFDIAPDGEYDIDPLTAV

EDGRAFSVRPKPFKVRCTPRPGVDLSPVLDKQ

 

>CYP504E1 Nectria haematococca

e_gw1.40.25.1

Necha1/scaffold_40:1293-2938

86% to AAJN01000191.1 Aspergillus terreus

72% to XM_654859.1 Aspergillus nidulans (pseudogene)

45% to e_gw1.24.52.1, 45% to 504A1 Aspergillus nidulans

46% to XM_743078.1 Aspergillus fumigatus

Change the gene model at the N-terminal

MLTAVAVALVTYVFYH (0)

FLYATDIPHIKGLPEIPGAVPVLGHLLQLGEDHASTCEKWWRQYNHSVFQI

KLGNTRAVVVNSFEDCKKMLLGNQNAVIDRPKLYTFHGVISSTQGFTIGSSPWDDSCRKKRKAAGTALGR

PALRNYYPMFDLESYCILRDMEKDSRNGEIEIDVRPYIQRYALNTTLTLCYGIRMDAVYDDLLREILHVG

SAISLLRSASENLQDYVPILRYLPNNEKNSRSKELRERRDAYLNLLLDKVRDMIKKGTDKPCISAAILKD

QETKLTGVEVSSICLSLVSGGFETIPGTLTSCLSSLSTKEGQLWQGRAYEDIKRHYPDVRDAWTSCYVEE

KIPYVNAIVKEAGRYYTVSSMSLPRKTVTEVNWNGASIPPKTMILINAQAGNH (1)

DVDHFGPDGGKFNPERW

LKNLDPPTEAESVGLAHLSFGTGSRACSGQYIASRLLYAALVRLLSSYKIVASEEMPPNTDYVEYNQFKT

ALVAIPREFKVKLIPRDTAMTAECLSAAEERTKEHYKE*

 

>CYP504E2 Aspergillus terreus NIH2624

AAJN01000191.1

N-term not clear, does not match other related genes

ATEG_07913.1

        MTTTHDTAFIPSLAYRIQDGQGSTILGIAGVIALLSYL (0)

189214  TTIATDIPFIKGLPEIPGAVPIFGHLLKLGDDHATVCEKWWRQYGHSVFQIRLGNTRAV  189390

189391  VVNSFEDCRKMLLGHQNAIIDRPTLYTFHGVISSTQGFTIGSSPWDASCRKKRKAAGTAL  189570

189571  GRPALRSYYPMFDLESYSIVRDLHRDSCDGEVELNVRPYIQRYALNTTLTLCYGIRMD  189744

189745  AVYDELLREILYVGSAISLLRSASENLQDYIPLLRYLPNNEKNARSKSLRERRDKYLDLL  189924

189925  LDKVRDMIKKGTDKPCISSAILKDEETKLNGVEVSSICLSLVSGGFETIPGTLTSAIGSL  190104

190105  STKEGQVWQDRAYEDIKRHYPDIRDAWTSCFVEEKVPYVNAIIKEAGRYYTVSAMSLPRK  190284

190285  TVTEVNWNGAIIPQKTMVLINAQAGNH (1)  190365

190412  DVDHFGPDGGKFDPERW

190463  LKTLDPPTERETSGLNHLSFGAGSRACSGQYIASRLLYAALVRILSSYKIVASETEPPNT  190642

190643  DYVDYNQFKTALVAIPRDFKVKLVPRDPGMTAECLETAELRTREHYKE*  190789

 

>CYP504E3 Phaeosphaeria nodorum

AAGI01000160.1

MEVLHLLQSLYCTPLGYVSLLALFGFCVASIYK (0)

15789  LLTRTEVPYIRGIPEIPGALPVTGHLTQLGDDHATICEKWWRQYGHSVFQVRLGNTRAV  15613

15612  IVSSFQDCKKILVSNPQGLIDRPKLYTFHGVISSTQGFTIGSSPWDESCRNKRKAASTTL  15433

15432  SKGMLKNYYEMFDLESYCIVRDLQRDSKGSALEVSIRPYIQRYALNTTLTLCYGIRMDA  15256

15255  VYDDLLREILHVGSAISLLRSASENYQDYIPILRYLPNNEKNRRSKELRERRDKYLNELL  15076

15075  DKVRNMIDKGTEKPCISAAILKDEETRLTGVEVSSICLSLVSGGFETIPGTLVSCIGSLS  14896

14895  TPEGQKYQETAYQEISKVYPDLEDAWSREFEKENVPYLNAIVKEAGRYYTVSNMSLPRKT  14716

14715  MSDVRWGEAIIPKGTMVLINAQAGNH (1)

       DVDYWGPDAGTFNPERWLEPPHPSSIPSIF

14497  TEKPTSSTSHLSFGAGSRSCPGAAIASKLISAALFRILSLYKIEASSTEPPNTDYVEYNA  14318

14317  IKSALVAIPRDFKIKLTPRDETGDLAREVLRMGEERTKNFYKE  14189

 

>CYP504E4P  Aspergillus nidulans add to nomen.

AN2347.3 76% to CYP504E2

MKTDYDITA*TVSLSQGYSAVLLFILVTLYLLYKYTLATDIPHIKN

LPVIPGALPIFGHLLKLGGDHATV*

EKWWRQYGHSVFQIRLANTRVVVVKSFDDCRKMLLSHQNALIDRPKLYTSYCVISSTQGYTIDSSPWDEPCRKKRKAAGTALGRPALRNY

HPMFDLESCCILR

TDLYRDSKDGEAEVSVRPYLQRYALNTTLTLCYGIRMDEV

YDEVLSEILYAGSAISLLHSAAENLQDYISLLRYVPNNEKKARSK

TRDRRDKYLDLLTRVRKMIERGTDKPCISAAILKVKE

(gap 40 aa)

QAWQDHAYEDIKRWYPDLQDTWTAAVTEERV

TYINAIIKEAARYYTVSSMSLPRK

VTEINWNGAIIPPKTMILINAQAGNR

DIDHLGPTGNRFNPER*LSS

 

>CYP504E5 Aspergillus niger

gw1.9.669.1|Aspni1

84% to CYP504E1 aa 219-331

ERWWRQYGHSVFQIKLGNTRAVVFNSFEDCRTVLLGHQNAIIDRPKLYTFHGVISSTQGFTIGSSPWDDSCKRKRKAAGT

ALGRPALRNYHPMFDLESYCIVRDLFRDSKNGQPGSVTGASADPTRSYIKRGAPPPDIALPHLHQLSXQSTLFTPKMGSL

LTQDMMSVKGRVALVTGGSSGIGRMIAQPSYQGLVTNGAKAYIVALPGDPIDQTVQDLNACGIESGGSAEGYACDLSSKD

SIYQLAKSISQKETKLDMLISNAGIRRDPPIACNVLTATLAELQESMWSSRESDWADTFRINTTAHYFLSVAFLPLLAAA

AD

 

>CYP504E6 Aspergillus niger

e_gw1.112.2.1|Aspni1

84% to CYP504E2 aa 279-590

ISLLRSASENLQDYIPILRYAPNNEKTARSKELRERRDAYLNLLLNKVRDMIKKGTDKPCISAAILKDEETKLSGVEVSS

ICLSLVSGGFETIPGTLTSVIGSLSTPEGQIWQDRAYEDIKRYYADPGDAWLSSIHEEKVPYVNAIVKEAGRYYTVSSMS

LPRKTATEVNWNGAIIPPKTMILINAQAGNHDVDHFGPDAGKFDPERWLKSVDPPSEKEIQGLPHLSFGAGSRACSGQVI

AIRLLYTALVRILSSYKIVASEDQPPNTDYVEYNQFKTALVAIPRDFKVRLIPRDDVLTRQCLDAAELRTKE

 

>CYP504E7 Mgr054 Mycosphaerella graminicola

71% to CYP504E1   Nectria haematococca

gw.1.2625.1 revised N-term

MELPKTPESNTSTQITFIAGLGFLFWWIYK (0)

WSTRTDIPKIAGIFEVPNAWPIVGHLLALGDDHATVCEASSNPHSIFQIRLGNTRAVVVNSFDDAKRMLLGFQSAVIDRPKLY

TFHGVISSTQGFTIGSSPWDESCKVRRKAAGQTLGRPAMKTYRPMF

DLETYCIIRDVVRDNKGGDEYLEIRPYLQRYALN

TTLTLCYGIRMDSVWDDMLREVLEVGSAISLLRSASENYQDYIPILRYLPNNEKNRRSKSLRDRRDRYLNALLEKVKEMI

EMGADKPCVSAAILKDEDTKLSGVEVSSICLSLVSGGFETIPGTLTSCIGSLSTPEGQLFQDRAYEDIKRRYPSIEDAWT

TALDAEEVPYVNAIVKEAGRYYTVSSMSLPRKAAADIDWDGAIIPKGTMILINAQAANHSEEHFGPDAKTFNPERWLSSL

EPPQEAPSTGLQHHSFGAGSRACSGQYIASRMLYTALIRLISSFKIVASEEEPPNVDYIEYNQFKSALVAVPRDFKVQMV

PRDPGALEKCLALAEQRTED

 

>CYP504E8P gw1.9.803.1|Mycgr3 N-term piece Mycosphaerella graminicola

68% to 504E7 new seq. not in Steve Kelly�s set

TDIKRINGIPEIPGAVPICVHLLKLGEDHASTCERWWRQYNHPVFQIRFGNTRAVVFNSFDACRDILVKHHSAVIDRPKL

YTFHGVINSTQGFTIGSSPWVASSKNKRKAAGTALARPALRDYHPMFDLESFCIVRDMHADSDCSNPQKEVSIRPYIQRY

ALNTTLTLCYGIRMNDIYDELLREILHVGSAIFLLRSASENLQDYIPALCYLPNNE*TARSKELRDRRDAYLDLLLNEVCEMIK

QGVDKPCISAAILKDEETKLAGVEVSSICLSLVSGGFETIPGTLTSCSDSLSTP

 

>CYP504E9P Mycosphaerella fijiensis

76% to CYP504E7 Mycosphaerella graminicola

synt_with_mycgr.1__200

MLGSDLETNLDFIT &

LTTSTDIPKISSIFEIPGAFPITGHLLELGDDHATVCE (0)

WQKYKKSVF*IRLGNTRAVVINSFEDCKRMLIGNQSAVIDRPKLYTFHR

LISSTQGFTIGSSPWDESCKNKRKAAGQTLGRPAMKGYQAMF (0)

DLETYTLIRDLVKDSSGDCGETYLNPR

PYLQRYALNTTLTLCYGIRMDSVWDDMLREILEVGSAISLLRSASENMQD*VP (1)

ILRYLPNNEKNQRSKSLRDRRDKYFNELLDRVREMIQH

GTDKPCVSAAILKDEETKLTGVEVSSICLSLVSEGFETIPGTLTSCLGSLATPEGQVFQERAYEDLKRHYPNVEDAWKQC

LESEDIPYINAIVKEAGRFYTVSSTSLPRKAAVDIPWDGALIPKGTMILINAQAGNHEVGHWGADAATFNPERWLEELDP

PKEKASSGLQHLSFGAGSRACSGQHIASRLLYSALVRLLSSFRIVASPDEPPNTDYVDYNAIKSALVAIPRDFKVRLISR

DEEALESCLRSGENRTRDAYAERSHGSGGHGRKLYQ*

 

>CYP504F1 Nectria haematococca

fgenesh1_pg.scaffold_33000056

Necha1/scaffold_33:187197-188955

44% to 504B1

MNLIEYFLRKELSLPALLGYAIAISVIALLVYLAQNEFTRHSSRIKHLPGPRGWPVVGNLFQ (0)

VPAETYRRWAQKHGPVFQIQLGNTTGVVVNSVDAAKKLFIGQRNAMNSRPTFHVFHGKVSKAVTSIGTSPWDESCKRR

RKLAAAGLNRPRVESYAPILNLESREFLRDLHNESKDGSIEIDFRQAVTRFSLNLSLTLNYGTRVSNVKS

LHDDPLLAEILYVESEISKFRDTSKNYANYIPLLRYLEPIASFLRLTSAPKSHAADIGRRRLEYNDVLLN

KLKDEVERGEDKPCIQGAVLREPESATLTRGELISVSLSMMA (0)

GADSNQPTIAWAILLLAHRPDIQEKAYH

AIKEAGVLDIPSNAYASSKVEYIDALTKEIARYFVVLKLALPKATYTDATWGPATIPPNTLVFLNSWACNR (1)

DPELFTEPEVFAPERWLPGSPDYAAHQFSFGIGGRMCVASLLAHRALYTVFLHLVAQFQIFPA

SGTTDEEIDPLKG

LKGRSFVATPRGFRARFVPREGQNLETWLRSPDGDI*

 

>CYP504G1 Candida guilliermondii

PGUG_05169.1

38-39% to many CYP504 sequences, also 39% to CYP5054A1 and 41% to CYP5054A2

MLLDLLFDFLNSYGYLLLVVSILIAAKSVFGAAYCTGIRKIKGIVEPPECEAFYGHLNVLGEDHATNLQTIGDRYNLKVF

QVRFGVERAVILHTFAEGFSWFVKNHQSALIDRPLFYTFHKLVSNTQGLTVGTSPWDENCKRRRLAITKYMTRTALQKIQ

DLIDLESCSFVKDIWRLTCEGSIGVDPHIAAQRMSFNITTMLCYAIRFDTIDSSLLLDILKIVSQVSSFRSTNNNLQDYV

PIYRYLPDKRREMAKEVSKKRDVWLNDFLNRAFERSEVRESFAGSMGTKKLSLEDLRSICVSLVSGGFETLASITSLLLA

QLSTENGKEWQETAYGELTDAYGNDGDKLWNSVTFEEKCPFVVALIRETLRHYPVIPLCPPRKTTREFEYEGAKIPKGVT

VILNCQAANHDKEHFGADADEFNPARWFDESLDINKPPFHFTFGAGSRACPGILLSHRALYATIGRMIFLFKIGPHGEKP

DLNYKTYAEDKTAQTWSPLPFKISLEPRGPVDKFLNRDHLQHLVELSYEHI

 

>CYP505A1 AB030037 Fusarium oxysporum CYP505

MAESVPIPEPPGYPLIGNLGEFTSNPLSDLNRLADTYGPIFRLR

LGAKAPIFVSSNSLINEVCDEKRFKKTLKSVLSQVREGVHDGLFTAFEDEPNWGKAHR

ILVPAFGPLSIRGMFPEMHDIATQLCMKFARHGPRTPIDTSDNFTRLALDTLALCAMD

FRFYSYYKEELHPFIEAMGDFLTESGNRNRRPPFAPNFLYRAANEKFYGDIALMKSVA

DEVVAARKASPSDRKDLLAAMLNGVDPQTGEKLSDENITNQLITFLIAGHETTSGTLS

FAMYQLLKNPEAYSKVQKEVDEVVGRGPVLVEHLTKLPYISAVLRETLRLNSPITAFG

LEAIDDTFLGGKYLVKKGEIVTALLSRGHVDPVVYGNDADKFIPERMLDDEFARLNKE

YPNCWKPFGNGKRACIGRPFAWQESLLAMVVLFQNFNFTMTDPNYALEIKQTLTIKPD

HFYINATLRHGMTPTELEHVLAGNGATSSSTHNIKAAANLDAKAGSGKPMAIFYGSNS

GTCEALANRLASDAPSHGFSATTVGPLDQAKQNLPEDRPVVIVTASYEGQPPSNAAHF

IKWMEDLDGNDMEKVSYAVFACGHHDWVETFHRIPKLVDSTLEKRGGTRLVPMGSADA

ATSDMFSDFEAWEDIVLWPGLKEKYKISDEESGGQKGLLVEVSTPRKTSLRQDVEEAL

VVAEKTLTKSGPAKKHIEIQLPSAMTYKAGDYLAILPLNPKSTVARVFRRFSLAWDSF

LKIQSEGPTTLPTNVAISAFDVFSAYVELSQPATKRNILALAEATEDKDTIQELERLA

GDAYQAEISPKRVSVLDLLEKFPAVALPISSYLAMLPPMRVRQYSISSSPFADPSKLT

LTYSLLDAPSLSGQGRHVGVATNFLSHLTAGDKLHVSVRASSEAFHLPSDAEKTPIIC

VAAGTGLAPLRGFIQERAAMLAAGRTLAPALLFFGCRNPEIDDLYAEEFERWEKMGAV

DVRRAYSRATDKSEGCKYVQDRVYHDRADVFKVWDQGAKVFICGSREIGKAVEDVCVR

LAIEKAQQNGRDVTEEMARAWFERSRNERFATDVFD

 

>CYP505A1 Fusarium oxysporum CYP505

98% to CYP505A1 Fusarium oxysporum AB030037, only 13 aa diffs

FOXG_04152

MAESVPIPEPPGYPLIGNLGEFTSNPLSDLNRLADTYGPIFRLRLGAKAPIFVSSNSLINEVCDEKRFKKTLKSVLSQVR

EGVHDGLFTAFEDEPNWGKAHRILVPAFGPLSIRGMFPEMHDIATQLCMKFARHGPRTPIDASDNFTRLALDTLALCAMD

FRFNSYYKEELHPFIEAMGDFLTESGNRNRRPPFAPNFLYRAANEKFYGDIALMKSVADEVVAARKASPSDRKDLLAAML

NGVDPQTGEKLSDENITNQLITFLIAGHETTSGTLSFAMYQLLKNPDAYSKVQKEVDEVVGRGPVLVEHLTKLPYISAVL

RETLRLNSPITAFGLEAIDDTFLGGKYLVKKGEIVTALLSRGHVDPAVYGNDADKFIPERMLDDEFARLNKEYPNCWKPF

GNGKRACIGRPFAWQESLLAMVVLFQNFNFTMTDPNYALEIKQTLTIKPDHFYINATLRHGMTPTELEHVLAGNGATSSS

THNIKAAANSDTKTGGSGKPMAIFYGSNSGTCEALANRLASDAPSHGFSATTVGPLDQAKQNLPEDRPVVIVTASYEGQP

PSNAAHFIKWMEDLNGNEMEKVSYAVFACGHHDWVETFHRIPKLVDSTLEKRGGTRLVPMGSADAATSDMFSDFEAWEDT

VLWPGLKEKYKISDEESGGQKGLLVEVSTPRKTSLRQDVEEALVVAEKTLTKSGPAKKHIEIQLPSAMTYKAGDYLAILP

LNPKSTVARVFRRFSLAWDSFLKIQSEGPTTLPTNVAISAFDVFSAYVELSQPATKRNILALAEATEDKATIQELERLAG

DAYQAEISPKRVSVLDLLEKFPAVALPISSYLAMLPPMRVRQYSISSSPFADPSKLTLTYSLLDAPSLSGQGRHVGVATN

FLSHLTAGDKLHVSVRASSEAFHLPSDAEKTPIICVAAGTGLAPFRGFIQERAAMLAAGRTLAPALLFFGCRNPEIDDLY

AEEFERWEKMGAVDVRRAYSRATDKSEGCKYVQDRVYHDRADVFKVWDQGAKVFICGSREIGKAVEDVCVRLAIEKAQQN

GRDVTEEMARAWFERSRNERFATDVFD

 

>CYP505A1 Fusarium verticillioides

98% to CYP505A1 Fusarium oxysporum CYP505

FVEG_07269

MAESVPIPEPPGYPLIGNLGEFTSNPLSDLNRLADTYGPIFRLRLGAKAPI

FVSSNSLINEVCDEKRFKKTLKSVLSQVREGVHDGLFTAFEDEPNWGKA

HRILVPAFGPLSIRGMFPEMHDIATQLCMKFARHGPRTPIDASDNFTRLAL

DTLALCAMDFRFNSYYKEELHPFIEAMGDFLTESGNRNRRPPFAPNFLY

RAANEKFYNDIALMKSVADEVVAARKASPSDRKDLLAAMLNGVDPQTGEKL

SDENITNQLITFLIAGHETTSGTLSFAMYQLLKNPEAYSKVQKEVDEVV

GRGPVLVEHLTKLPYISAVLRETLRINSPISAFGLEAIDDTFLGGKYLVKK

GEIVTALLSRGHVDPAVYGSDADKFIPERMLDDEFARLNKEYPNCWKPF

GNGKRACIGRPFAWQESLLAMVVLFQNFNFTMTDPNYALEIQQTLTIKPVH

FYINATLRHGMTPTELEHVLAGSGGTSSSNHNIKSAANSDVKAGSGKPM

AIFYGSNSGTCEALANRLASDAPSHGFSATTVGPLDQAKQSLPEDRPVVIV

TASYEGQPPSNAAHFIKWMEDLDGNEMEKVSYAVFACGHHDWVETFHRI

PKLVDSTLEKRGGTRLVPMGSADAATSDMFSDFEAWEDTVLWPGLKEKYKI

SDEESGGQKGLLVEVSTPRKTSLRQDVEEALVVAEKTLTKSGPAKKHIE

IQLPSAMTYKAGDYLAILPLNPKSTVARVFRRFSLAWDSFLKIQSEGPTTL

PTNVAISAFDVFSAYVELSQPATKRNILALAEATEDKATIQELERLAGD

AYQAEISPKRVSVLDLLEKFPAVALPISSYLAMLPPMRVRQYSISSSPFAD

PSKLTLTYSLLDAPSLSGQGRHVGVATNFLSQLTAGDKLHVSVRASSEA

FHLPSDAEKTPIICVAAGTGLAPFRGFIQERAAMLAAGRTLAPALLFFGCR

NPEVDDLYAEEFERWEKMGAVDVRRAYSRAADKSEGCKYVQDRVYHDRA

DVFKVWDQGAKVFICGSREIGKAVEDVCVRLAIEKAQQNGKDVTEEMARAW

FERSRNERFATDVFD*

 

>CYP505A2 N. crassa AABX01000234.1 cont3.292 NCU05185.1 (version3)

28427 MSSDETPQTIPIPGPPGLPLVGNSFDIDTEFPLGSMLNFADQY (1) 28555

28609 GEIFRLNFPGRNTVFV (0) 28656

28709 TSQALVHELCDEKRFQKTVNSALHEIRHGIHDGLFTARNDE

PNWGIAHRILMPAFGPMAIQNMFPEMHEIASQLALKWARHGPNQSIKVTD

DFTRLTLDTIALCSMDYRFNSYYHDDMHPFIDAMASFLVESGNRSRRPAL

PAFMYSKVDRKFYDDIRVLRETAEGVLKSRKEHPSERKDLLTAMLDGVDP

KTGGKLSDDSIIDNLITFLIAGHETTSGLLSFAFVQLLKNPETYRKAQKE

VDDVCGKGPIKLEHMNKLHYIAAVLRETLRLCPTIPVIGVESKEDTVIGG

KYEVSKGQPFALLFAKSHVDPAVYGDTANDFDPERMLDENFERLNKEFPD

CWKPFGNGMRACIGRPFAWQEALLVMAVCLQNFNFMPEDPNYTLQYKQTL

TTKPKGFYMRAMLRDGMSALDLERRLKGELVAPKPTAQGPVSGQPKKSGE

GKPISIYYGSNTGTCETFAQRLASDAEAHGFTATIIDSLDAANQNLPKDR

PVVFITASYEGQPPDNAALFVGWLESLTGNELEGVQYAVFGCGHHDWAQT

FHRIPKLVDNTVSERGGDRICSLGLADAGKGEMFTEFEQWEDEVFWPAME

EKYEVSRKEDDNEALLQSGLTVNFSKPRSSTLRQDVQEAVVVDAKTITAP

GAPPKRHIEVQLSSDSGAYRSGDYLAVLPINPKETVNRVMRRFQLAWDTN

ITIEASRQTTILPTGVPMPVHDVLGAYVELSQPATKKNILALAEAADNAE

TKATLRQLAGPEYTEKITSRRVSILDLLEQFPSIPLPFSSFLSLLPPMRV

RQYSISSSPLWNPSHVTLTYSLLESPSLSNPDKKHVGVATSYLASLEAGD

KLNVSIRPSHKAFHLPVDADKTPLIMIAAGSGLAPFRGFVQERAAQIAAG

RSLAPAMLFYGCRHPEQDDLYRDEFDKWESIGAVSVRRAFSRCPESQETK

GCKYVGDRLWEDREEVTGLWDRGAKVYVCGSREVGESVKKVVVRIALERQ

KMIVEAREKGELDSLPEGIVEGLKLKGLTVEDVEVSEERALKWFEGIRNE

RYATDVFD* 31858

 

>CYP505A3   Aspergillus oryzae

           GenEMBL AB078738

MSTPKAEPVPIPGPRGVPLMGNILDIESEIPLRSLEMMADTYGP

IYRLTTFGFSRCMISSHELAAEVFDEERFTKKIMAGLSELRHGIHDGLFTAHMGEENW

EIAHRVLMPAFGPLNIQNMFDEMHDIATQLVMKWARQGPKQKIMVTDDFTRLTLDTIA

LCAMGTRFNSFYSEEMHPFVDAMVGMLKTAGDRSRRPGLVNNLPTTENNKYWEDIDYL

RNLCKELVDTRKKNPTDKKDLLNALINGRDPKTGKGMSYDSIIDNMITFLIAGHETTS

GLLSFAFYNMLKNPQAYQKAQEEVDRVIGRRRITVEDLQKLPYITAVMRETLRLTPTA

PAIAVGPHPTKNHEDPVTLGNGKYVLGKDEPCALLLGKIQRDPKVYGPDAEEFKPERM

LDEHFNKLPKHAWKPFGNGMRACIGRPFAWQEALLVIAMLLQNFNFQMDDPSYNIQLK

QTLTIKPNHFYMRAALREGLDAVHLGSALSASSSEHADHAAGHGKAGAAKKGADLKPM

HVYYGSNTGTCEAFARRLADDATSYGYSAEVESLDSAKDSIPKNGPVVFITASYEGQP

PDNAAHFFEWLSALKGDKPLDGVNYAVFGCGHHDWQTTFYRIPKEVNRLVGENGANRL

CEIGLADTANADIVTDFDTWGETSFWPAVAAKFGSNTQGSQKSSTFRVEVSSGHRATT

LGLQLQEGLVVENTLLTQAGVPAKRTIRFKLPTDTQYKCGDYLAILPVNPSTVVRKVM

SRFDLPWDAVLRIEKASPSSSKHISIPMDTQVSAYDLFATYVELSQPASKRDLAVLAD

AAAVDPETQAELQAIASDPARFAEISQKRISVLDLLLQYPSINLAIGDFVAMLPPMRV

RQYSISSSPLVDPTECSITFSVLKAPSLAALTKEDEYLGVASTYLSELRSGERVQLSV

RLSHTGFKPPTELSTPMIMACAGSGLAPFRGFVMDRAEKIRGRRSSGSMPEQPAKAIL

YAGCRTQGKDDIHADELAEWEKIGAVEVRRAYSRPSDGSKGTHVQDLMMEDKKELIDL

FESGARIYVCGTPGVGNAVRDSIKSMFLERREEIRRIAKEKGEPVSDDDEETAFEKFL

DDMKTKERYTTDVFA

 

>CYP505A3 Aspergillus flavus

99% to CYP505A3   Aspergillus oryzae 4 aa diffs

AFL2G_09091 revised

MSTPKAEPVPIPGPRGVPLMGNILDIESEIPLRSLEMMADTYGPIYRLTTFGFSRCMISSHELAAEVFDEERFTKKIMAG

LSELRHGIHDGLFTAHMGEENWEIAHRVLMPAFGPLNIQNMFD (1)

EMHDIATQLVMKWARQGPKQKIMVTDDF

TRLTLDTIALCAMGTRFNSFYSEEMHPFVDAMVGMLKTAGDRSRRPGLVNNLPTTENNKYWEDIDYLRNLCKELVDTRKE

NPTDKKDLLNALINGRDPKTGKGMSYDSIIDNMITFLIAGHETTSGLLSFAFYNMLKNPQAYQKAQEEVDRVIGRRRITV

EDLQKLPYITAVMRETLRLTPTAPAIAVGPHPTKNHEDPVTLGNGKYVLGKDEPCALLLGKIQRDPKVYGPDAEEFKPER

MLDEHFNKLPKHAWKPFGNGMRACIGRPFAWQEALLVIAMLLQNFNFQMDDPSYNIQLKQTLTIKPNHFYMRAALREGLD

AVHLGSALSASSSEHADHAAGHGKAGAAKKGADLKPMHVYYGSNTGTCEAFARRLADDATSYGYSAEVDSLDSAKDSIPK

NGPVVFITASYEGQPPDNAAHFFEWLSALKGDKPLDGVNYAVFGCGHHDWQSTFYRIPKEVNRLVGENGANRLCEIGLAD

TANADIVTDFDTWGETSFWPAVAAKFGSNTQGSQKSSTFRVEVSSGHRATTLGLQLQEGLVVENTLLTQAGVPAKRTIRF

KLPTDTQYKCGDYLAILPVNPSTVVRKVMSRFDLPWDAVLRIEKASPSSSKHISIPMDTQVSAYDLFATYVELSQPASKR

DLAVLADAAAVDPETQAELQAIASDPARFAEISQKRISVLDLLLQYPSINLAIGDFVAMLPPMRVRQYSISSSPLVDPTE

CSITFSVLKAPSLAALTKEDEYLGVASTYLSELRSGERVQLSVRPSHTGFKPPTELSTPMIMACAGSGLAPFRGFVMDRA

EKIRGRRSSGSMPEQPAKAILYAGCRTQGKDDIHADELAEWEKIGAVEVRRAYSRPSDGSKGTHVQDLMMEDKKELIDLF

ESGARIYVCGTPGVGNAVRDSIKSMFLERREEIRRIAKEKGEPVSDDDEETAFEKFLDDMKTKERYTTDVFA*

 

>CYP505A4 Magnaporthe grisea MG05401.4  53% to CYP505A2 AACU01000504 cont2.993

MSHNHNPRANNRRGSIREKLKMGTAKNPPKAVPEQDDDEDVKLVPIPQPS

RAPFVGNLPDLDVKFPLDSMVNLANKYGSIFKLDLPGLEFVTVSDWDLVH

EVCDDSRFKKSIKGDLEELRNAVHDGLFTSSGEDEENWGIAHRVLMTAFG

PLSIRNMFDEMHEVASQLALKWARQGPHEPLDVSGDTTRLALDTVALCSM

GFRFNSYYRQDLHPFIKAMNEVLDEAGRRANRFMPSVFYHSHNKKFRENI

KLLRTTAREVLDARKTQKGPEKRRDLLTAMLDGVDPKTGKKMTDESIIDN

LITFLVAGHETTAATLSFALYNLAKFPEVSRKAQKEVDDVVGKGAVKLEH

VPKLKYISALIRETLRLNAPITAFGREAIGDQVLGGKYLLKDKTQIVCFL

TKSQSDPVIWGPDADEFKPERMLDDSFDRMQKKHPHCWAPFGTGVRGCIG

RPFAWQEMVLCLAVLLQNYNFVMYNPAYALRLAQSLTIRPKDLYLRAISR

 

>CYP505A5 Magnaporthe grisea MG01925.4  55% to 505A2 AACU01000373 cont2.362

MAATQSRDWASFGVSLPSTALGRHLQAAMPFLSEENHKSQGTVLIPDAQG

PIPFLGSVPLVDPELPSQSLQRLARQYGEIYRFVIPGRQSPILVSTHALV

NELCDEKRFKKKVAAALLGLREAIHDGLFTAHNDEPNWGIAHRILMPAFG

PMAIKGMFDEMHDVASQMILKWARHGSTTPIMVSDDFTRLTLDTIALCSM

GYRFNSFYHDSMHEFIEAMTCWMKESGNKTRRLLPDVFYRTTDKKWHDDA

EILRRTADEVLKARKENPSGRKDLLTAMIEGVDPKTGGKLSDSSIIDNLI

TFLIAGHETTSGMLSFAFYLLLKNPTAYRKAQQEIDDLCGREPITVEHLS

KMPYITAVLRETLRLYSTIPAFVVEAIEDTVVGGKYAIPKNHPIFLMIAE

SHRDPKVYGDDAQEFEPERMLDGQFERRNREFPNSWKPFGNGMRGCIGRA

FAWQEALLITAMLLQNFNFVMHDPAYQLSIKENLTLKPDNFYMRAILRHG

 

>CYP505A6 Magnaporthe grisea MG10879.4  59% to 505A2 AACU01001802 cont2.2099

MATSDDQTVPIPEPQGLPLLGNIGAIDNEFPLGSLLSMASKYGEIWRMRF

PGRSLVFVSTQALVNEVCDEKRFYKSINPTLKEVRGGTHDGLFTAYPDEE

NWGIAHRILMPAFGPLSIRSMYDEMHDIASQLALKWARYGSNSPIMVTDD

FTRLTLDTLALCSMGFRFNSYYSTEMHPFIDAMADFLVECGTRGRRPPLP

SIFYRTQDQKFDSDIEILRKTAREVLEARKAGEGADRRDLLHAMLSGVDS

KTGKKMTDESIMDNLITFLIAGHETTSGLLSFVFYELMKHPEFYEKAQRE

VDEVCGKGPITVEHMSKLPYLNAVLRETLRVHATIPNITVAAREDDVLGG

KYKVSKDEVIVLLLAKSQVDPRVWGKDADEFVPERMLDENFERITKEFPN

SWKPFGNGMRACIGRPFAWQEALLVMAMLLQNFNFVLAPNYQYALKQTLT

LKPKDLYMRAILR

 

>CYP505A7 Fusarium graminearum FG01972.1 AACM01000107 FGcontig1.107_scaffold1

MAESVPIPEPPGYPLIGNLGEFKTNPLNDLNRLADTYGPIFRLHLGSKTP

TFVSSNAFINEVCDEKRFKKTLKSVLSVVREGVHDGLFTAFEDEPNWGKA

HRILIPAFGPLSIRNMFPEMHEIANQLCMKLARHGPHTPVDASDNFTRLA

LDTLALCAMDFRFNSYYKEELHPFIEAMGDFLLESGNRNRRPAFAPNFLY

RAANDKFYADIALMKSVADEVVATRKQNPTDRKDLLAAMLEGVDPQTGEK

LSDDNITNQLITFLIAGHETTSGTLSFAMYHLLKNPEAYNKLQKEIDEVI

GRDPVTVEHLTKLPYLSAVLRETLRISSPITGFGVEAIEDTFLGGKYLIK

KGETVLSVLSRGHVDPVVYGPDAEKFVPERMLDDEFARLNKEFPNCWKPF

GNGKRACIGRPFAWQESLLAMALLFQNFNFTQTDPNYELQIKQNLTIKPD

NFFFNCTLRHGMT

 

>CYP505A8 Aspergillus nidulans AN6835.1 55% to 505A1 505 clan

MAEIPEPKGLPLIGNIGTIDQEFPLGSMVALAEEHGEIYRLRFPGRTVVV

VSTHALVNETCDEKRFRKSVNSALAHVREGVHDGLFTAKMGEVNWEIAHR

VLMPAFGPLSIRGMFDEMHDIASQLALKWARYGPDCPIMVTDDFTRLTLD

TLALCSMGYRFNSYYSPVLHPFIEAMGDFLTEAGEKPRRPPLPAVFFRNR

DQKFQDDIAVLRDTAQGVLQARKEGKSDRNDLLSAMLRGVDSQTGQKMTD

ESIMDNLITFLIAGHETTSGLLSFVFYQLLKHPETYRTAQQEVDNVVGQG

VIEVSHLSKLPYINSVLRETLRLNATIPLFTVEAFEDTLLAGKYPVKAGE

TIVNLLAKSHLDPEVYGEDALEFKPERMSDELFNARLKQFPSAWKPFGNG

MRACIGRPFAWQEALLVMAMLLQNFDFSLADPNYDLKFKQTLTIKPKDMF

MKARLRHG

 

>CYP505A9 Nectria haematococca

fgenesh1_pg.scaffold_12000374 [Necha1:90392]

Necha1/scaffold_12:985898-989367

77% to 505A7, 79% to 505A1

MAESVPIPEPPGYPLIGNLGEFTTSPLADLKRLADTY (1)

GPIFRLRLPGKSPIFAANNAFVNELCDEKRFQKTLKSVLG (0)

TVREGVHDGLFTAYNDEPNWGKAHRILLPAFGPLSIRSMFDEMHDIATQMCMKFARHGPQTPI

NASDDFTRLALDTLALCSMGFRFNSYYREELHPFIQAMGDFLTESGVRNRRPTFAPNFLYRAANEKFLAD

IKVMKDLAAEVVANRKERPNDRKDLLTAMLEGVDPQTGEKLSDDNIGNQLVTFLIAGHETTSGTLAFSFY

NLLKHPEAYEKAQQEVDQVIGRGPITVEHLTKLPYLSA (0)

ILRETLRLNSPIPGFGVEAIEDTFLGGKYLVKKGEIVSCMLSKAHLDPVVYGEDAEAFRPERMLDENFER

LQKEHSNCWKPFGNGKRACIGRPFAWQEALLALAMLLQNFNFTMDDSNYQLQIQETLTIKPKHFNMRATL

RYGMTPTELERVLAGRGADSHATSGTKSSAAAGTKGAGGKPISIYYGSNSGTCEALAQRLASDAPGHGFA

AKNIGPLDEAKQNLPEDHPVVIVTASYEGQPPSNAAHFINWMERLNGQEMEKVSYAVFACGHHDWVETFH

RIPKLVDATLEKRGGTRLVPMGSADAAVSDMFSDFEAWEDETLWPALKEKYGGDGGDSQAGSQRGLLVEL

STPRKTRLRQDVEEALVVSEKTLTASGPPKKNIEIQLPTGMTYKAGDYLAILPLNPKTTVGRVFRRFKLA

WDVFLKIHSEGPTTLPTDTEISAYDVFSAYVELSQPATKR (0)

NILALVEATEDKAVIEELEKLTGDAYAEEISAKKVSVLDLLEKYPAVALPISSYLGMLPPMRVRQY (2)

SISSSPLANSSKLTLTYSLLDEPSLSGQGRHVGVATHFLSSLSAGEKLHVSVRPSVAAFHLPSDAE

NTPLICIAAGTGLAPFRGFVQERAAMLAAGRNLAPAMLFFGCRNPDIDDIYADEFDRWEKMGAVSVRRAY

SRATDKSEGCKYVQDRLYHDRADVFKLWDQGAKVYICGSREVGKAVEAVCVRLVVEWSEEHMECTSTEEK

AREWFEKHRNERFATDVFD*

 

>CYP505A10P Nectria haematococca

fgenesh1_pg.scaffold_160000001

Necha1/scaffold_160:4628-11288

MAETAPIPEPPSYPLIGNWGEFTTTPATDFNRLAVTY (1)

 

>CYP505A11 Nectria haematococca

fgenesh1_pg.scaffold_53000030 [Necha1:96758]

Necha1/scaffold_53:84132-87603

59% to 505A7, 60% to 505A1

58% to fgenesh1_pg.scaffold_12000374

MAETVEIPTPAGLPLLGNIKSIDPEFPLGSMVSLAEQH (1)

GEIYRLKFPGRTVVIVSTQALVNETCNEKRFKKCVNSALT (0)

EIREGVHDGLFT (0)

AKLGEENWGIAHRVLMPAFGPLSIQRMFDEMHDIASQLALKWARYGPDSP

IMVTDDFTRLTLDTLALCSMGYRFNSYYSPVLHPFIEAMGDFLTEAGEKPRRLPLPGIVYRERDRKYQQD

IETMRSTAKEVLDARKAGGSTRKDLLTAMLEGVDTKTGKKMTDESIMDNLITFLIAGHETTSGLLSFAFY

QLLTHPEAYQLAQKEVDQVVGKGPIQVGHLSKLPYLNGVLRETLRINATIPLFTVEAFEDTLLGGKYPVK

AGETILNLLAKSQLDPTVFGDDANEFKPERMFDDNFARLNKEFPNSWKPFGNGMRACIGRPFAWQEALLV

MVMLLQNFNFVLDPSYELGFKQTLTIKPKDMHMRAILRDNLTPTSLERRLAGLPVSEREEANGVNGKSSD

AKDGVPMTVLYGSNSGTCESLAQRVATDAAEHGFHVTKIDCLDTANGNLPTDQPVVIVTASYEGQPPDNA

GHFVAWVEKADQETKPLEGVSYAVFGCGHKDWVQTFHRIPKLVDSNLEQLGATRLVNIGLTDASENMVFS

DFEAWEDNILWPALEARYNPESKKSSADKGLSVKSSNFRTLTLRQDVKEATVVETKVLTSSTDTKSKKRH

IEVELPEGTQYTAGDYLAVLPINPQEVVRRALRRFKLPMDAHLEISSSTPTVLPTDTSVSALDVLSSYVE

LSQPATKR (0)

NLLAIAEAASNEDTKAALTSLSEERYVEEISNKRVSVLELLERYPSVDLPIGDFLQMLPSIRIRQY (2)

SISSSPLWNQSRATITFSVLKGPASSGQGIYNGVATSYLDSLADGDTLQVAIRSSPAAFSLPSNPE

QTPIICIAAGSGLAPFRGFIQQRAIMIESGRKLAPGLLFFGCRAPDQDDLYHDEFDVWQKLGAVDVRRAY

SRHGDGCKHVQDRIWQDKEDFIQLWEKGATVYVCGSRAVADSVREVMIKLKTEMEKRSGGEMGLEEAKKW

FDEQRNVRYVMDVFD*

 

>CYP505A12 Nectria haematococca

fgenesh1_pg.scaffold_23000101 [Necha1:93347]

Necha1/scaffold_23:253431-256834

57% to 505A7, 56% to 505A1

57% to fgenesh1_pg.scaffold_12000374

Note: unusual exon 1 codes for 2 amino acids, this matches with

other CYP505 sequences.

MP (2)

NITPIPEPPALPWLGHIAEFKSEDSLQDLTRLHEIY (1)

GDIYRLRFPEGKSYILLGSNKLVNEVCDETRF

QKSINNELWEVRNLANDGLFTAKPGEENWGIAHRVLMPAFGPVNIRGMFDEMYDIATQLALKWARHGPSQ

AILAADDFTRLAFDTIALCSMDFRFNSFYRDKAHPFISSMGDVLTESGKRFQRPSVAKVFYRAITKKYFQ

DIKMMREVADEVVAARRANPDSKKKDLLAAMMNGVDPKTGKKMNDVSITDNLITFLVAGHETTSGTLSFL

FYSLLKNPDCYQKAQKEVDDIMGKEPITAEKIFKLRYIPA (0)

ALRETLRVCSPIPGVGMEALKDTLLDYKYP

IAKGDVLSPFLTVAHLDPRVFGEDAHLFRPERMLDENFERLQKEHPNCWKPFGNGVRACIGRPFAWQEML

LAVALLLQNFNISMDDPGYNLRIAETLTVKPKDFYIRASLRHGMSPLDLEHKLRGTTAEPVAATNGAAKD

EKHQVLESDGKRISIFYGSNSGTCESLAQRLAADAFSHGFTASCIESLDVAKENLPTNQPVVIITSSYEG

QPPDNAAHFVSWLKNLEGKPLDKVSYAVFGVGHREWSATFHKVPKLVNTELEDHGAKRVAEIGLTDTSDR

DPFTDFETWEDNILWPALQKEGPDSEPKFAIPQSDLSVCVSMPRSSTLRQDVNEAIVTATRDLSGPGVPL

KKHFEIALPSEVSYRAGDYLCILPINPRETVSRVFRHFKLSWDAVLTIEGEKRVALPMSQPTSAWNVLSA

YVELAQPATKRNILSLAEYTADGETKRSLQDLAGENLQEEVTKKRVSVMDLLERFPKIELPLAPFLTMLP

PMRIRQY (2)

SIASSPLKSPHHVSVTYSILDEPSLSGQGRHIGVATSYLSNLSTNDLVHVAIRPSHVAFHLPL

TPETTPIICVAAGAGVGPFRAFIEERAQQMAAGRKLAPALLFFGCRGKTDDLYREEFDAWEKQGAVIVKR

AYSRETSEETKNCKYVQHRMVEEKHEVAELWDRGAKLYVCGSRDMGKAVEEACVGLLQELNDMSEKEARM

FVEEVRNQRFATDVFT*

 

>CYP505A13  Aspergillus fumigatus Af293

           GenEMBL XP_754698.1 also EAL92660.1

           fatty acid hydroxylase predicted

           60% to 505A8

MSESKTVPIPGPRGVPLLGNIYDIEQEVPLRSINLMADQYGPIYRLTTFGWSRVFVSTHELVDEVCDEER

FTKVVTAGLNQIRNGVHDGLFTANFPGEENWAIAHRVLVPAFGPLSIRGMFDEMYDIATQLVMKWARHGP

TVPIMVTDDFTRLTLDTIALCAMGTRFNSFYHEEMHPFVEAMVGLLQGSGDRARRPALLNNLPTSENSKY

WDDIAFLRNLAQELVEARRKNPEDKKDLLNALILGRDPKTGKGLTDESIIDNMITFLIAGHETTSGLLSF

LFYYLLKTPNAYKKAQEEVDSVVGRRKITVEDMSRLPYLNAVMRETLRLRSTAPLIAVHAHPEKNKEDPV

TLGGGKYVLNKDEPIVIILDKLHRDPQVYGPDAEEFKPERMLDENFEKLPKNAWKPFGNGMRACIGRPFA

WQEALLVVAILLQNFNFQMDDPSYNLHIKQTLTIKPKDFHMRATLRHGLDATKLGIALSGSADRAPPESS

GAASRVRKQATPPAGQLKPMHIFFGSNTGTCETFARRLADDAVGYGFAADVQSLDSAMQNVPKDEPVVFI

TASYEGQPPDNAAHFFEWLSALKENELEGVNYAVFGCGHHDWQATFHRIPKAVNQLVAEHGGNRLCDLGL

ADAANSDMFTDFDSWGESTFWPAITSKFGGGKSDEPKPSSSLQVEVSTGMRASTLGLQLQEGLVIDNQLL

SAPDVPAKRMIRFKLPSDMSYRCGDYLAVLPVNPTSVVRRAIRRFDLPWDAMLTIRKPSQAPKGSTSIPL

DTPISAFELLSTYVELSQPASKRDLTALADAAITDADAQAELRYLASSPTRFTEEIVKKRMSPLDLLIRY

PSIKLPVGDFLAMLPPMRVRQYSISSSPLADPSECSITFSVLNAPALAAASLPPAERAEAEQYMGVASTY

LSELKPGERAHIAVRPSHSGFKPPMDLKAPMIMACAGSGLAPFRGFIMDRAEKIRGRRSSVGADGQLPEV

EQPAKAILYVGCRTKGKDDIHATELAEWAQLGAVDVRWAYSRPEDGSKGRHVQDLMLEDREELVSLFDQG

ARIYVCGSTGVGNGVRQACKDIYLERRRQLRQAARERGEEVPAEEDEDAAAEQFLDNLRTKERYATDVFT

 

>CYP505A13 Neosartorya fischeri

97% TO CYP505A13  Aspergillus fumigatus = ortholog

NFIA_067930

MSESKTVPIPGPRGVPLLGNIYDIEQEVPLRSINLMADQYGPIYRLTTFGWSRVFVSTHELVDEVCNEERFTKVVTAGLN

QIRNGVHDGLFTANFPGEENWAIAHRVLVPAFGPLSIRGMFDEMYDIATQLVMKWARQGPTVPIMVTDDFTRLTLDTIAL

CAMGTRFNSFYHEEMHPFVEAMVGLLQGSGDRARRPALLNNLPTSENAKYWNDITFLRNLAQELVEARRKNPEDKKDLLN

ALILGRDPKTGKGLTDESIIDNMITFLIAGHETTSGLLSFLFYYLLKTPHAYKKAQEEVDSVIGRRKITVEDMSKLPYLN

AVMRETLRLRSTAPLIAVHAHPEKNKEDPVTLGGGKYILNKDEPIVIILDKLHRDPQVYGPDAEEFKPERMLDENFEKLP

KNAWKPFGNGMRACIGRPFAWQEALLVVAILLQNFNFQMDDPSYDLHIKQTLTVKPKDFHMRATLRHGLDATKLGIALSG

TADSAPPESSGAASRVRKQAAPPPGQLKPMHIFFGSNTGTCETFARRLADDAVGYGFAADVQSLDSAMQNVPKDEPVVFI

TASYEGQPPDNAAHFFEWLSALKENELEGVNYAVFGCGHHDWQATFHRIPKAVNQLVAEHGGNRLCDIGLADAANSDMFT

DFDSWGESAFWPAITSKFGGGKSDEPKSSSSLQVEVSTGMRASTLGLQLQEGFVVDNQLLSAPDVPAKRMIRFKLPSDMS

YRCGDYLAVLPVNPSSVVRRAIRRFDLPWDAMLTIRKPSHAPKGSTSIPLDTPISAFELLSTYVELSQPASKRDLTALAD

AAVTDADVQAELRYLASSPTRFTEEIVKKRMSPLDLLIRYPSIKLPVGDFLAMLPPMRVRQYSISSSPLADPSECSITFS

VLNAPSLAAASLPPAERAEAEQYMGVASTYLSELKPGERAHIAVRPSHSGFKPPMDLKAPMIMACAGSGLAPFRGFIMDR

AEKIRGRRSSVGADGQLPEVEQPAKAILYVGCRTKGKDDIHAAELAEWAQLGAVDVRWAYSRPEDGSKGRHVQDLMLEDR

EELVSLFDQGARIYVCGSTGVGNGVRQACKDIYLERRRQLRQEARERGEEVPAEDDEDAAAEQFLDNLKTKERYATDVFT*

 

>CYP505A14  Aspergillus oryzae

           GenEMBL BAE56245.1

           61% TO 505A3

           revised 3/19/2009

MEETKLIPIPEPRGLPLLGNILDVDSEAPEKSFQRLAETYGPIFRLNLAGASRVFISTYELVDEICDEER

FTKVVTAGLREIRNGVQDGLFTADYPGEDNWAIAHRVLVPAFGPLMIRAMYEDMYDIASQLALKWARQGS

SATIMANDDFTRLTLDTIALCSMGTRFNSFYSEDLHPFIKAVATLLQGSSDRTFRSTLLNNLPTRENKKY

WSDISLLRTLSQELVDARRNNPIDKKDLLNALILGQDLQTGQHLSDDSIINNMITFLVA (1)

GHETTSATLTFLFYYLLKNPHAYQRAQEEVDTVVGQRKIIVEDLSKLPYIAASLRETLRLQAPVPLIAFHPHPTK

NHEDPVTLGKGKYALNNDEPVVLIMGKVHRDPKVFGDDAEEFKPERMLDKNFEDLPKNAWKPFGNGMRGC

IGRPFAWQEMLLVVAMLLQNLNFEMENPSYDLRIKQSLSIKPDGFQMKATLRRGLDAAKLASVLNSGGDL

LSHAPQILNGEYKPNTDLRFHLRPMHIFFGSNTGTCEALARRLAKDSMGYGFATRVESLNSAMENIPRDN

PVIFITATYEGQPPDNAAHFFEWLNGLKKAELDGVNYCVFGCGHHDWSATFLRIPKATNDLIEKHGGTRL

CDMGMADAANSDMFSDFDTWSELILWPAINLKFGRASSEGDVQSKSALHVDVSSSMRAFTLGLQLQEGYV

LENKLLTTPDVPAKRMLRFKLPPDTTYQCGDYLIVLPVNPAHVVCRAIRRFNISWDSMLTVRKPSHASDG

ITNMPLETPISAFELFSTYVELSQPASKRDLITLADAATTDTDAQAELQSLASSPNRFTEEVINNRLSPL

DILIRHPSINLPLSTFLEMLPPLRARQYSISSSPLASRSDCTITFSVLNSPHLSTENKRFVGVASTYLSE

LQAGDRVQISIRASNNKGFKPPLKEETPMIMACAGSGLAPFRGFIMDRAERIRGRGTELLSDDDHPEIGK

PARAILYIGCRTKGKDDIHASELDEWTRQGAVDVRWAYSRPTDRSQGRHVQDLLFEDRNELLELIDQGAR

IYVCGGMSVGQGIRQVFKDMFIERCREVLENGSDGDEDVAAEEYLDSLKTEERYATDVFT

 

>CYP505A14  Aspergillus flavus

99% to CYP505A14  Aspergillus oryzae

AFL2G_01234

MEETNLIPIPEPRGLPLLGNILDVDSEAPEKSFQRLAETYGPIFRLNLAGASRVFISTYELVDEICDEERFTKVVTAGLR

EIRNGVQDGLFTADYPGEDNWAIAHRVLVPAFGPLMIRAMYEDMYDIASQLALKWARQGSSATIMANDDFTRLTLDTIAL

CSMGTRFNSFYSEDLHPFIKAVATLLQGSSDRTFRSTLLNNLPTRENKKYWSDISLLRTLSQELVDARRNNPIDKKDLLN

ALILGQDLQTGQHLSDDSIINNMITFLVAGHETTSATLTFLFYYLLKNPHAYQRAQEEVDTVVGQRKIIVEDLSKLPYIA

ASLRETLRLQAPVPLIAFHPHPTKNHEDPVTLGKGKYALNNDEPVVLIMGKVHRDPKVFGDDAEEFKPERMLDKNFEDLP

KNAWKPFGNGMRGCIGRPFAWQEMLLVVAMLLQNLNFEMENPSYDLRIKQSLSIKPDGFQMKATLRRGLDAAKLASVLNS

GGDLLSRAPQILNGEYKPNTDLRFHLRPMHIFFGSNTGTCEALARRLAKDSMGYGFATRVESLNSAMENIPRDNPVIFIT

ATYEGQPPDNAAHFFEWLNGLKKAELDGVNYSVFGCGHHDWSATFLRIPKATNDLIEKHGGTRLCDMGMADAANSDMFSD

FDTWSELILWPAINLKFGRASSEGDVQSKSALHVDVSSSMRASTLGLQLQEGYVLENKLLTTPDVPAKRMLRFKLPPDTT

YQCGDYLIVLPVNPAHVVCRAIRRFNISWDSMLTVRKPSHASDGITNMPLETPISAFELFSTYVELSQPASKRDLITIAD

AATTDTDAQAELQSLASSPNRFTEEVINNRLSPLDILIRHPSINLPLSTFLEMLPPLRVRQYSISSSPLASQSDCTITFS

VLNSPHLSTENKRFVGVASTYLSELQAGDRVQISIRASNNKGFKPPLKEETPMIMACAGSGLAPFRGFIMDRAERNRGRR

TELLSDDDHPEIGKPARAILYIGCRTKGKDDIHASELDEWTRQGAVDVRWAYSRPTDRSQRRHVQDLLFEDRNELLELID

QGARIYVCGGMSVGQGIRQVFKDMFIERCREVLENGSDGDEDVAAEEYLDSLKTEERYATDVFT*

 

>CYP505A15 Aspergillus niger

fgenesh1_pm.C_scaffold_7000162|Aspni1

72% to CYP505A13

MGSVKPVPIPGPKGVPFLGNVYDIDQEMPVRSFELMADQYGPIYRLTTFGLSRVVVSTQELVDEVCNEERFAKSVDGGLG

QLRNLLHDGLFTARYPGEENWALAHRVLIPAFGPLSIRGMFDEMQDIANQLVMKWARQGPETPIVVTEDFTRLTLDSIAL

CAMGTRFNSFYHDSMHPFVEAMVGGMQVASYRAQRPSFLNQLPTSENNAFWNDISYMRNLAKELIEDRRKHPSDKKDLLN

ALILGRDPQSGKGLSDESIIDNMITFLIAGHETTSGMLSFVFYYLLKNAHAYKKAQEEVDRVIGRRKITVDDLSKLPYIN

AVMREALRLNPPASVMRVHAHPTKNKEDPVTLGNGRYVLNKDEPITVLLPKLHRDPKVYGADAEEFKPERMLDENFNSLP

RNSWKPFGNGMRGCIGRPFAWQEGLLAIALLLQNFNFQLDDPSYDLRIKQTLTIKPKDFKMRATLRHGLDPITLEPALNS

GSVPRESSTVASRERKKDAALSEGDMKPMHIFFGSNTGTCEALARRLADDAVAWGFSAKVNSLDSATENVPKGDPVVFIA

ASYEGQPADNAAHFFEWLSAANDNELEGINYAVFGCGHHDWTATFYRIPKAINELVKEHGGNRLCDIGLVDTAIADMFTD

FDNWSESALWPAISSKFGSKSQGQAGKKASSGLQVELSSEKRVTTLGLQLQEGYVIENKLLTAPDVSAKRLVRIKLPSDM

TYQCGDYLAVLPVNPTSIVRRAMRRFKLPWDAFLTVRKSSQTQNGPSAIPTDTPISAFALLSSYVELSQPASKRDLTALA

DAAVADEESQAELRYMANSPSRFNEEVVTKRLSLLDILDRYPSIGLPLGDFLAMLPPMRVRQYSISSSPLTDPTECSITV

SVLNAPSLSGTDEPYMGVASTYLSILQADERIHIAVRPSHSGFKPPADLNTPMIMACAGSGLGPFRGFVMDRAEKILSRS

DSITEKPAPAILYVGCRTKGKDDIHAEELAEWSRQGIVDVRWAYSRPSDGSVAQHIQDRITSDRDEVVSLFEQGARIYVC

GSAEVGKAVRQAFKNIYVDVKQERRRVALETKQEEVPSEVNDEDVEKFFDDLKASMRYATDVFT*

 

>CYP505A16 Fusarium oxysporum

57% to CYP505A12   Nectria haematococca

FOXG_17221, no ortholog in F. verticillioides

MALSCDESTPIPEPRGLPFLGNVTE

FRSEDSLQDLDRLHDTYGEIYRLRFPKVGSCVIVGSQKLVNELCDEKRFRKAIQAELAEIRLAAGDGLFTAREGEV

SWDIAHRVLMPVFGPTAIRSMFDEMYDVVSQMTLKWARYGPTSPISASEDFTRLALDTIALCSMGYRFNSFYKDTTHPFV

QSMSDSLVELGNRSLRPSWARIFYRSSERKLAKDINFMRTTSEDLIKARKADPNADQRKDLLAAMMNGVDPRTGSRMDDE

SIINNLLTFLIAGHETTSGTLSFAFYSMLKNPDAYLKAQQEVDSIMGRDPITVDKLFQLKYVPAVLRETLRQCSPIPGIT

LEAREDTLLDGKYHIRKGEAVAAIFSKSHLDPLVYGADAKEFKPERMMDENFDRLQQEFPNCWKPFGNGVRACIGRAFAW

QEMLLATSMLLQNFDFYLDDPLYKLKIAETLTIKPKDFLMGASLRHNMTPLDLESHLRGTNNKQGIPAAHSQSPPSTIKA

SIAATPISIYYGSNSGTCEILARRLASNASSHGFSVMVIDTLDTARNRLPQDHPVVIFVASYEGQPPDNAAHFVNWIQSM

TGNEVQKVHYVVFGVGNSEWRQTFHRIPKLIDDELGKRGGERLVTLGLTNVAEKDPFLDFEIWESKELWPALEKKYPDVT

GGTPEALVGSMGLSVDISVSRASALRKSVKEAIVTGVRDLAAPGLPRKRHIEMKLPSGCSYEVGDYLEVLPMNPRDAVTR

ALRRFKLPWDAVLTIAGSEITSLPIGIPVSAWDVLSSYVELAFLSTRKDTLVLASYAQSEKVRTEIELLASDVKFKSEIE

DKRVSILDLLERFPSVELPIATYLGMLQPIRTRQYSISSSPLKDPSHATLTYSVLETPSLSGQGLYMGVASNFLASLKPD

DRLQVSIRRSNIAFHLPLAPDTTPIICVAAGAGLAPFRGFIQQRSIQMKADRILAPALLIFGCRRRDDDLYREEFDAWEG

EGAVVVKRAYSRDAKLTAGCKYVQDRVIEQQEYIRKLWNQGGNVYVCGSRQVGDAVEGALGSILSGVQGGDREDGKRFLD

NFRNKRFVMDIFS*

 

>CYP505A17 Fusarium oxysporum

86% to CYP505A11

FOXG_17581 revised

MAGTVEIPSPSGLPLLGNITSIDPEFPLGSMVSLAEQH (1)

GEIYRLKFPGRSVVIVSTQALVNETCNEKRFKKCVNSALT (0)

EIRDGVHDGLFTARLGEENWGIAHRVLMPAFGPLSIQRMFDEMHDIASQLALKWARYGPNSPIMVTDDFTRLTLDTLAL

CSMGYRFNSYYSPVLHPFIEAMGEFLTEAGEKPRRLPLPGIVYRDRDRKYQQDIEIMRDTAREVLEARKADGSTRKDLLA

AMLEGVDTKTGKKMTDESIMDNLITFLIAGHETTSGLLSFAFYQLLTHPDAYQLAQNEVDRVVGKGPIQVEHLSKLPYLN

GVLRETLRLNATIPLFTVESFEDTLLGNKYPVKAGETIINLLAKSQLDPTVFGDNADKFRPERMFDENFARMNKEFPNSW

KPFGNGMRACIGRPFAWQESLLVMVMLLQNFNFVLDPNYHLGFKQTLTIKPKDMYMRAVLRDNLTPTTLERRLAGLPVLA

TGEANGTNGESADAEDGIPITILYGSNSGTCESLAQRVATDAAQHGFRVTKIDCLDMANGNLPADQPVVIITASYEGQPP

DNAGHFVAWVENAAQEEKPFKDVSYAVFGCGHKDWVLTFHRIPKLVDRSLAKHGATRLVDMGLADVSQNMVFSDFETWED

NILWPALEKRYKPGCKTMLTDKGLSVKSCTFRTLTLRQDVKEATVVCTKTLTSNIDVKSKKKHIEIQLPEGTGYTAGDYL

AVLPINPHEIVRRALRRFRLPMDAHLKISSSTPAALPTDTSVSAMDVLSSYVELSQPATKR (0)

NLLIIAEAASDVETKAALTSLSQESYVEEISNKRVSVLEILERYPSVELSIGDFLQMLPSIRIRQY (2)

SVSSSPLWNQAHATITFSVLKGPAFSGHGTYNGVATSYLDSLAEGDTLQVAIRSSPAAFSLPSNPECTPIICVAAGS

GLAPFRGFIQQRAIIYDSGRTLAPALLFFGCRAPDQDDLYRDEFDSWQRLGAVQVRRAYSRHREGCRYVQDRIWQDKEDF

IELWNKGAMVYVCGSRAMADSVKEVMLKVKTEIEKRSGGDIGLDEARKWFDEQRNVRYVMDVFD*

 

>CYP505A18 Aspergillus clavatus

87% to CYP505A13 Aspergillus fumigatus

ACLA_037140

MTESKTIPIPGPKGVPLLGNIYDIEQEVPLRSINMMADHYGEIYRLTTFGRSRVFLNTHELVNEACDEERFSKVVTAGLN

EIRNGIHDGLFTAHFPGEENWAIAHRVLVPAFGPLSIRGMYDDMYDIATQLVMKWARQGPHVPIMVTDDFTRLTLDTIAL

CAMGTRFNSFYHEEMHPFVEAMVGLLQGSGDRARRPALLNNLPTSENAKYWSDIEFLRKLAQELVDNRRQNPEDKKDLLN

SLILGRDPKSGQGLSDSSIIDNMITFLIAGHETTSGLLSFLFYYLLKNPRAYQKAQEEVDAVIGRRKITVEDLSKLPYIN

AVMRETLRLRSTAPLIALHAHPEKNKEDPVTLGNGKYVLNKDEAIVIVLDKLHRDPKVYGPDAEEFRPERMLDEEFDKLP

KNAWKPFGNGMRACIGRPFAWQEALLVVAILLQNFNFQMDDPSYDLHIKQTLTIKPKDFHMRAALRHGLDATQLGIMLSG

SVDSVPPESAGAASRSRKPQAAAPPSGQLKPMHIFFGSNTGTCETFARRLADDAVGYGFAAEVQSLDSAKENIPKEDPVV

FITASYEGQPPDNAAHFFEWLSGLKGKELEGINYAVFGCGHHDWHATFHRIPKAVNELVGERGGNRLCDLGLADAAHSDM

FTDFDSWGESAFWPAITSKFGGGKSETGTKATSNLQVEISSGMRASTLGLQLQEGFVIENQLLSQPGVPAKRMVKFKLPS

DMTYQCGDYLAVLPVNPSSVVRRAIRRFDLPWDAMLTIRKSSQASKGSTSIPLDTPVSAFDLLSTYVELSQPASKRDLNV

LADAAAADADVQAELRYLASSPSRFTEEIVKKRLSPLDILIRYPSIKLPVGDFLAMLPPMRVRQYSISSSPLADPSECCI

TFSVLNAPSLAAASLPPQEQEEAEQYLGVASTYLSELKPGDRAHISVRPSHSGFKPPMDLKTPMIMACAGSGLAPFRGFV

MDRAEKIRGRHSSIGSDKEIPETAKPAKAILYVGCRTKGKDDIHAAEFAEWAQLGAVEVRWAYSRPEDGSKGRHVQDLML

EDRAELVDLFDQGARIYVCGSTGVGNGVRQACKEMYLERRRQVRQEKRERGEDVAEDDEDAAAEQFLDNLKTKERYATDV

FT*

 

>CYP505A19 Aspergillus terreus

65% to CYP505A18

ATEG_01555.1

MSARIPIPTPKGIPILGNITELAQDVPEYSLYRLADQHGPIFRLTFLNQSIVFVNSYELANEEIRNCVHDGLFTANYPGE

ENREIAHRVLVPAFGPLMIRGMFDDMYDIASQLALKWARQGPNTAITVNEDFTRVSMDTIALCALGTRFNSFYRDEMPHF

IATMRNLLQASSARALRPADLVDARLQDPVDKKDLLNALILGRDPQTGQKLTTDSIVNNMITFLIAGHETTSGMLSFLFY

DLLKHPHAYRKAQDEVDRVIGRRKITVEDLTKLPYITAVMRETLRLHCPVTMLVVHAHPIRNNEDPVTLGNGRYELYKDE

PVAIHIGKIHRDPAVYGDDADEFKPERMLDGKFEKLPKNAWKPFGNGMRACIGRPFAWQEVLVVVAMLLQKINFQMVNPS

YDLRLKQAMTLKPDGFTFRAAIRHGLDATRLSAFLNRGSDLTEYAGALGRECNMEMVLGNHLKPMRIFFGSNMGTCEAFA

WRLAHDALQYGFRAEVKALDAARENLSRGDPVAFMTASYEGQPPDNATHFFEWLKGLEGDKLEGVNYAVSGCGHHDWQAT

FHSIPKAIDRLVHQRAGTRLCEIGTADAANSDMFTDFDSWGESVFWPAVMSKFGVQVNGTAKEASNLEIQIRPGRRVSAL

GLQLQEGQVLENRLLTAPSVPEKRMLRFKLPPDMTYQCGDYLAILPVNPPNVVRWAIRRFNLSWDSMLTVWKPTDASGLI

AVPLETPISALELFSTYVELSQPASKRDLKYLADAANGDTAVQNELRSLASDATRFVDEIVQKRVSPLDILLRHPVIDLP

LNTFLTMLLLMRPGEKAHMTVRRSTFGFKPPLDLQTPMIMACAGSGLAPFRGFIMDRAEKIRGRQLTGAPHPDIRGLAKA

ILYVGCRTKGVDDIHAEELAEWERLGAVDVRWVYSRPAGDSLSGSSPHEGSDGADRSRWRRHVQDQIVHDREELVALLER

GARIFVCGGTGVGQGVRQALKRIYLRQRWDGCRLRIDTDEGDPSRTADDEDAAAERFLEMLKTKERYATDVFT

 

>CYP505A20 Mycosphaerella fijiensis

55% to CYP505A9 Nectria haematococca

e_gw1.27.10.1

MAAIDERDTVPIPQPPGYPIIGNLLDVSGDVPIMNLADLGKKYGDIFSLSILGKRQIFVASAELMRELCDEKRFEKAINA

ALCQLRNGIHDGLFTAHGGEENWSIAHRTLVPAFGPLNIKDMFDSMKDIASQLVLKWARHGTTYKIPVAVDFTRLTLDTL

ALCAMDYRFNSFYTEHMHPFIDSMVNFLKYADVRSKRPGWMTSFYKADEAKWWSDIEYMRNLSAELIQQRRDQPNNSKDL

LNAMIKGKDPRTQKGLPESSIIDNMITFLIAGHETTSGMLSYAFYCLLKNPDTYRKAQEEVDRVIGKENIEAHHLDKLPY

ITAVLRETARLHSTAPSISLSPKSEQGEVIGGKYHISHGEVARVLLHNVHRDPAIYGEDAEEFRPERMLDDNFSRFTPYW

KPFGNGARGCIGRPFAWQEMMLVLPMLLQYFNFTLDDPSYVLHTAFTLTIKPKDLFIRATLREGWTPLSIERHLAGSTAA

DIKHGPNVPAATSESDDKGIPMTILYGSNSGTCEAFAQTLAAGARAHGFKASKVDTLDTAKQNLPAKEPVVIVTASYEGE

PTDNAAHFYNWLQNLSSDEKLKTSYTVFGCGHSDWKQTFHRVPKAIDSMLETAGGERMCEMGSADAARGDMMSAFQAWED

ETLWPALRQKFGGQEGDTSAASSLAQAVSIEVFSKRASNLRADVSEAKVLSTKTLTAAGARVKKHIELRLPSSLTYRAGD

YLAVLPLNPAETVHRVAKRFSLPWDAMLKITSRTGTTLPTDHPISAHNLFSAYLELSQPATKRNLAMLIEAAGDDQTKKE

LSDLATGDFQANITNKRISLLDLLERYPEIQLPLSAFVASLISMRVRQYSISSSPLSDPHVITLTYAVLDEPSLSGVGRH

VGVASHHLSQLQPGDMVHVAVKPSPAAFHLPSSADTTPVIMIAAGAGLAPFRGFIQERASQIGSGRKKLAPANLYFGCRH

PAQDDLYREEFDFWEKIGAVSVVRRSFSRAADLSEGHRHVDQVILADRRRLVELWDQGARVYVCGSRGLGESVKQAFLKI

AKDEAEAEGKEVSGEDLEKWFGLLRNERYSTDVFA*

 

>CYP505B1 AF155773 Gibberella moniliformis fumonisin biosynthetic gene cluster

also Fusarium verticillioides FVEG_00317

MSATALFTRRSVSTSNPELRPIPGPKPLPLLGNLFDFDFDNLTK

SLGELGKIHGPIYSITFGASTEIMVTSREIAQELCDETRFCKLPGGALDVMKAVVGDG

LFTAETSNPKWAIAHRIITPLFGAMRIRGMFDDMKDICEQMCLRWARFGPDEPLNVCD

NMTKLTLDTIALCTIDYRFNSFYRENGAAHPFAEAVVDVMTESFDQSNLPDFVNNYVR

FRAMAKFKRQAAELRRQTEELIAARRQNPVDRDDLLNAMLSAKDPKTGEGLSPESIVD

NLLTFLIAGHETTSSLLSFCFYYLLENPHVLRRVQQEVDTVVGSDTITVDHLSSMPYL

EAVLRETLRLRDPGPGFYVKPLKDEVVAGKYAVNKDQPLFIVFDSVHRDQSTYGADAD

EFRPERMLKDGFDKLPPCAWKPFGNGVRACVGRPFAMQQAILAVAMVLHKFDLVKDES

YTLKYHVTMTVRPVGFTMKVRLRQGQRATDLAMGLHRGHSQEASAAASPSRASLKRLS

SDVNGDDTDHKSQIAVLYASNSGSCEALAYRLAAEATERGFGIRAVDVVNNAIDRIPV

GSPVILITASYNGEPADDAQEFVPWLKSLESGRLNGVKFAVFGNGHRDWANTLFAVPR

LIDSELARCGAERVSLMGVSDTCDSSDPFSDFERWIDEKLFPELETPHGPGGVKNGDR

AVPRQELQVSLGQPPRITMRKGYVRAIVTEARSLSSPGVPEKRHLELLLPKDFNYKAG

DHVYILPRNSPRDVVRALSYFGLGEDTLITIRNTARKLSLGLPLDTPITATDLLGAYV

ELGRTASLKNLWTLVDAAGHGSRAALLSLTEPERFRAEVQDRHVSILDLLERFPDIDL

SLSCFLPMLAQIRPRAYSFSSAPDWKPGHATLTYTVVDFATPATQGINGSSKSKAVGD

GTAVVQRQGLASSYLSSLGPGTSLYVSLHRASPYFCLQKSTSLPVIMVGAGTGLAPFR

AFLQERRMAAEGAKQRFGPALLFFGCRGPRLDSLYSVELEAYETIGLVQVRRAYSRDP

SAQDAQGCKYVTDRLGKCRDEVARLWMDGAQVLVCGGKKMANDVLEVLGPMLLEIDQK

RGETTAKTVVEWRARLDKSRYVEEVYV

 

>CYP505B2 Aspergillus niger

fgenesh1_pg.C_scaffold_1000650|Aspni1

62% to CYP505B1

MSDAKIIPIPGPRPLPLLGNILDFDLDNLTQSLDRLSKAHGPIYALTFGSSTEIMVTSRRIAEELCDETRFCKLVAGGLE

KMKPVVGDGLFTAQSDDPKWAIAHRILMPLFGTMKIREMFNDMKDICEQMCLKWARLGESFPLDVCKNFTTLTLDTISLC

TIDYRFNSFYRDGKEDPFVEAVIAVMTDAFTQSNLPDFVNNWFRHKAMADFHDHAQNLRRRTEEIIQERRRSPVERNDLL

NAMLTAVDPKTGERLSDQSVVDNLLTFLIAGHETTSSLLSFCFYYLLENPDVLQKARDEVDQVIGDGPMMVEHLQKLPYI

ESILRETLRLRDPGPGFYLKPLKDDILDGKYYVKKDQPIFIVFDSVHRDPETYGSDANNFRPSRMSQENFEKLPPCAWKP

FGNGVRSCIGRPFAWQQSLLAVAMILQNFDLIKDETYKLKYHVTMTVRPVGFHMKVRLRRQRRATDFAQKLHAPVASQVP

LINHHQSIRPNTVEGSRITILHGSNAGSCEALALRLAADAGEYGFTRSKIEPLGSAVGKLPTDNPVIIITASYNGEPADE

ASDFVAWLKTAGRDELSGVSFSVFGCGHRDWASTYFAVPKLLDAEMERAGGRRIAPLGTADSATSDLFTDLEKWSVTHLF

PGLGNTEHIRRDAQGVKVSSSCQVMLSDPPRVTLRKGFVPTSITESRCLSQPGVPEKRHLELRLPDGFTYQPGDHLYRVL

SRFHLEGDMLLTLTRSQSLGLPVNTPISASDLFGAYVELRQIASPKGILALADSTPDSCTQTILRDLAGDLFKVEIQDKY

VSVLDLLERFPTINPSIGLFLSLLTPMRPRAYSFSSSPNEKPGYGTLTYTVVGSARSTGNRIELLSLFTPSCDPLHSTTP

LYSGLSPLVMVAAGTGLAPFRGFIQERRFRQLAGKKVGPALLFYGCRGRDLDDMYRDEMDGYEKEGVVSIHRAYSRVKGA

DFRYIDSAIARFLDHVVSLWASGGVVRVCGGKKMSNSVFEVLGPALLERDRLDGKTDVTDVAEWKRDLPRGRYVEEIFL*

 

>CYP505C1 Magnaporthe grisea MG07953.4  42% to 505A1

AACU01000977 cont2.1474

MSSMTATETTAKASQTEQQSKCPVLGPSGPGCPYSKGEKIPGPTAYPLIG

SIPHLDLKAPFESMLAIQKQYGPIFQMVFAGKREISVCTRELAHEVCDET

RWHKLVTSGVSTLRQVVQDALFTAHHGSRQWGISHRILKPIFGPLSVRSM

FDEMSDVAEQLCLKWARHGPDHAIDVAGDYTRLTLDTIALCMMDYRFNSF

YRDGQHHPFVKHMVAILSEADIQAMLPDWAGVFRPRAMRKFKKDIQLMTD

LCRGMVEARRANPVKRRDFLNAMLQNSDPETGEKLDDEEIVRNLITFLVA

GHETTSGMLCFATYYLLKHPETLAKAQKEVDDVVGTGPVTATHLGQLPYL

DGVFREALRLMPTAVAFYVTPYKPEMLGGKYLVEPGEAVCLLLDPIHRDR

AVYGPDADEWKPERMLQEKFEALPADAWKPFGNGQRICLGMPFTWQESKL

AMAMLLQNFDLSMDDPDYKLKIKHLLTIKPEGFNIRAKLR

 

>CYP505C2 Fusarium graminearum FG07596.1 AACM01000315 FGcontig1.315_scaffold4

MAIKDGGKKSGQIPGPKGLPVLGNLFDLDLSDSLTSLINIGQKYAPIFSL

ELGGHREVMICSRDLLDELCDETRFHKIVTGGVDKLRPLAGDGLFTAQHG

NHDWGIAHRILMPLFGPLKIREMFDDMQDVSEQLCLKWARLGPSATIDVA

NDFTRLTLDTIALCTMGYRFNSFYSNDKMHPFVDSMVAALIDADKQSMFP

DFIGACRVKALSAFRKHAAIMKGTCNELIQERRKNPIEGTDLLTAMMEGK

DPKTGEGMSDDLIVQNLITFLIAGHETTSGLLSFAFYYLLENPHTLEKAR

AEVDEVVGDQALNVDHLTKMPYVNMILRETLRLMPTAPGFFVTPHKDEII

GGKYAVPANESLFCFLHLIHRDPKVWGADAEEFRPERMADEFFEALPKNA

WKPFGNGMRGCIGREFAWQEAKLITVMILQNFELSKADPSYKLKIKQSLT

IKPDGFNMHAKLRNDRKVSGL

 

>CYP505C3  Aspergillus oryzae

          GenEMBL BAE63564.1

          54% to 505C2, 50% to 505A8, 45% to 505A3, 47% to 505B1

          revised 3/18/2009

MRQNDNEKQICPIPGPQ

GLPFLGNILDIDLDNGTMSTLKIAKTY

YPIFKFTFAGETSIVINSVALLSELC

DETRFHKHVSFGLELLRSGTHDGLFTAYDHEKNWELAHRLLVPAFGPLRIREMFPQMHDIAQQLCLKW

QRYGPRRPLNLVDDFTRTTLDTIALCAMGYRFNSFYSEGDFHPFIKSMVRFLKEAETQATLPSFISNLRVRA

KRRTQLDIDLMRTVCREIVTERRQTNLDHKNDLLDTMLTSRDSLSGDALSDESIIDNILTFLVAGHETTS

GLLSFAVYYLLTTPDAMAKAAHEVDDVVGDQELTIEHLSMLKYLNAILRETLRLMPTAPGFSVTPYKPEI

IGGKYEVKPGDSLDVFLAAVHRDPAVYGSDADEFRPERMSDEHFQKLPANSWKPFGNGKRSCIGRAFAWQ

EALMILALILQSFSLNLVDRGYTLKLKESLTIKPDNLWAYATPRPGRNVLHTRLALQTNSTHPEGLMSLK

HETVESQPATILYGSNSGTCEALAHRLAIEMSSKGRFVCKVQPMDAIEHRRLPRGQPVIIITGSYDGRPP

ENARHFVKWLQSLKGNDLEGIQYAVFGCG (1)

HHDWSTTFYKIPTLIDTIMAEHGGARLAPRGSADTAED

DPFAELESWSERSVWPGLEAAFDLVRHNSSDGTGKSTRITIRSPYTLRAAHETAVVHQVRVLTSAETTKK

VHVELALPDTINYRPGDHLAILPLNSRQSVQRVLSLFQIGSDTILYMTSSSATSLPTDTPISAHDLLSGY

VELNQVATPTSLRSLAAKATDEKTAEYLEALATDRYTTEVRGNHLSLLDILESYSVPSIEIQHYIQMLPL

LRPRQYTISSSPRLNRGQASLTVSVMERADVGGPRNCAGVASNYLASCTPGSILRVSLRQANPDFRLPDE

SCSHPIIMVAAGSGIAPFRAFVQERSVRQKEGIILPPAFLFFGCRRADLDDLYREELDAFEEQGVVTLFR

AFSRAQSESHGCKYVQDLLWMERVRVKTLWGQDAKVFVCGS

VRMNEGVKAIISKIVSPTPTEELARRYIAETFI

 

>CYP505C3 Aspergillus flavus

99% to CYP505C3 Aspergillus oryzae

AFL2G_10943

MRQNDNEKQICPIPGPQ (1)

GLPFLGNILDIDLDNGTMSTLKIAKTY ()

YPIFKFTFAGETSIVINSVALLSELCDETRFHKHVSFGLELLRSGTHDGLFTAYDHEKNWELAHRLLVPAFGPLRIREMFPQMHDIAQQLCLKW (2)

QRYGPRRPLNLVDDFTRTTLDTIALCAMGYRFNSFYSEGDFHPFIKSMVRFLKEAE

TQATLPSFISNLRVRAKRRTQLDIDLMRTVCREIVTERRQTNLDHKNDLLDTMLTSRDSLSGDALSDESIIDNILTFLVA

GHETTSGLLSFAVYYLLTTPDAMAKAAHEVDDVVGDQELTIEHLSMLKYLNAILRETLRLMPTAPGFSVTPYKPEIIGGK

YEVKPGDSLDVFLAAVHRDPAVYGSDADEFRPERMFDEHFQKLPANSWKPFGNGKRSCIGRAFAWQEALMILALILQSFS

LDLVDRGYTLKLKESLTIKPDNLWAYATPRPGRNVLHARLALQTNSTHPEGLMSLKHETVESQPATILYGSNSGTCEALA

HRLAIEMSSKGRFVCKVQPMDAIEHRRLPRGQPVIIVTGSYDGRPPENARHFVKWLQSLEGNDLEGIQYAVFGCG

HHDWSTTFYKIPTLIDTTMAEHGGARLAPRGSADTAEDDPFAELESWSERSVWPGLEAAFNLARHNSSDGTGKSTRITIRSPYTL

RAAHETAVVHQVRVLTSAETTKKVHVELALPDTMNYRPGDHLAILPLNSRQSVQRVLSLFQIGSDTILYITSSSATSLPT

DTPISAHDLLSGYVELNQVATPTSLRSLAAKATDEKTAEYLEALATDRYTTEVRGNHLSLLDILESYSVPSIEIQHYIQM

LPLLRPRQYTISSSPRLNRGQASLTVSVMERADVGGPRNCAGVASNYLASCTPGSILRVSLRQANPDFRLPDESCSHPII

MVAAGSGIAPFRAFVQERSVRQKEGIILPPAFLFFGCRRADLDDLYREELDAFEEQGVVTLFRAFSRAQSESHGCKYVQD

LLWMERVRVKTLWGQDAKVFVCGSVRMNEGVKAIISKIVSPTPTEELARRYIAETFI*

 

>CYP505C4 Aspergillus flavus

46% to CYP505C3

AFL2G_00477

Note: this seq has no ortholog in A. oryzae

MSGREDHEIPGPKPWPILGNGLDFPSEGYTDTLIELGLKYYPIYKLTFAGSVEVMVNSVALTNELCDESRFSKVVGPTLE

ELRSAAGSGLFTAYNGEPEWETGHRLLAPVFGPTKIRKMFDHMYEMVEQLSLKWERYGSTYPIEVADDFSRVTLDTIALC

GMSFRFNSFYRDGTFHPFVDSMNRWLKNSDTMGSTPRILKSFLFRAQRQYKYDIHLMRQTCLDLIERRKKDQSEHEDLLD

SLLKGVDPTTGARLSEETCVDNLITFLIAGHETTAGLLGFVFYYLAKYPHVQRKAQEEVDRVYGDGSITVNDLQKLRYIT

AILREALRLNPTAPSWGVSPHRDEIIGGKWHVKKGQPLNIMLHSVHRDREVYGPTADEFDPERMLDEAFERLPPNAWKPF

GNGKRGCIGRAFAWQEALLITSYLLHKFYFTLHDPNYTLVLQEALTVKPKGCRILAQPRGKKPTASSATTNDSLRGASGR

VSSPSVPRSSGPPLLVLYGSNAGTCEALGRQLARDIESRSSYTCTLAELDSYVGKLPRDQPVLILTGSYDGAPPSNAVKF

VKWLERPIVESLKGVSYAVFGCGHKDWRATLYKVPAAIDELLAQKGAHSLAPLFKVDTGSDDAFVQLDMWIERDLTGALD

MANTASGGTDSLRVFLQDPPNHRAGYVETVVCDVKLLTTPTTAKKIQVDLMVPTGLAYSVGDSISILPLNPKHTIQRALS

RFHLAWDTYLKLENDNLTHLPTEYPISVADLLGSFVELGQTATLGNMKTLIDATDDWTTKQALLSLRDAHEDHIADKHVS

VLDLLEQFPTIPISIEGYLSMLPLLRPRIYSIASDPQWQPGLLSIIASVIDEPHWRSDEDDLYRSELDGFEREGIVEVKR

AYSREVHSSDTRGGRYVQDRLELEKEDVIELWKLGARVYVCGGDGMARGVRAVLTRILEETGEGGDELLVAPRYVTEIFS*

 

>CYP505D1 ug.73.17.1 Phanerochaete chrysosporium
MTHEIPCPPAWPFLGHMTSIDPEYPTLSLHLFTKQYGEIYRLRLPGRDLVVVNSQELVHEVSDDKRFKKSPKGGLQELRPLIGDSLLTADYPREENWGIAHRVISPSFNPIGLRGFFDDMVDVISQLVLKWERFGPHYKIDIAEDFTAATFEVIALCCASYRMNTFYTGGTHPVATAVVDYGVEGFARGKRGRLLSWLMRSATAKFEQDKETLLQYADELLEERKAHPTDRKDVLWAMMNRADPVTGKKMTDLSVKQNLLTLLTAGHETTSAFMSIIIYYLIKYPEAMRKLREEIDTVLGDRQMTADDLARLPYLLAVMRETLRLTPVAPGRVIEAIEATTLKGGQYAIDKGQDILVAVHSSHRDPKVWGDDVDDFRPERMLDGKFEALPPDSWQPFSAGLRACIGRAIAWQEAQIMITFLVQHFTFTLADPQYELRIKQAFTLRVHDLYVHARRRTDRRGCVTLLPPAPAPGVGLAEAKGAPHDGGEGALPMHVFYGSNMGTCEAFAQRIVADAGRHGFKASLAALDAAVANLPTDGPVVIVTASYEGQPPDNAAHFVEWATNMRGSGAPALAGVVYALFGCGNRDWVQTYQRVPTLVDGALAAAGAERLLPRAEGDAGSGGFFEAFARWEGALWAALETRYATMKSGSAEGAVDVEVLDAGVSRADVLRQPDTMMGTVLENRVLTAQGAPVKRHIEFKLPEQVTYKAGDYLTVLPMNPPRDVRRAMARFGLLPDQEVTIRTKTPSSLPTGRPISVYTLLSAYVELSQPATTRDLRFLSEAAKSEAEKLVFKELAENYTECVLTGRLSVLDILEAHPNVDVPFGAFLQLLPSMRARQYSISSSPLCDPTRASLTIRVFEAPTSPGRKDPLLGVASTYLGGLHPGDRVQLAVRPCKTAFRLPADPAVPLVLVCAGAGLAPMRGFLQERALQKEGGRDVGKSLLFFGCRHPEEDYLYRDEDLKKWVELGIVDVRVAFSRAQDQSLGCKHVQDRLWHDRTDVMDACDKGAKLYLCGSAKMAAGVKDKLVLVVQDAMQLEHAAAVEQFNTMMAGRFATDVFE
 
>CYP505D2 pc.73.4.1 Phanerochaete chrysosporium
MTEPIPTPPSVPFLGHIPLLDREVPMLSLALLAEQYGDIYRLIFPGRSSIAIASQELVHEVSDDKRFRKTVQGPLGEVRAVAGDGLFTADVPGEENWDIAHRILMPAFSFMKIRDMFDDMVDVVAQMVVKWERFGPRFRIDPAVDFTALTLEAISLTTMSYRMNAFYTFVQNGIHPFAKAMNEFLQESGGRSRRGRVLSAFMRGATAKWEQNRDLMMKYVDDNARSSARKDVLDLMMNEKDPVTGRKMTELSIKQNLLTFLIAGHETTSGMLTFTIYYLLKYPAVMRKLREEIDTMIGDRPMTVDDVNKMPYLTAVMRESLRLGPSVPGRMIESLKDQTLKNGKYAVAKGEILVVCNFIAQRDSKVFGDDADEFKPERMMDGKFEALPPDAWQPFGAGVRGCIGRAFAWQEVQIVLVYLLQHFNLAFADPNYDLRLKQTLTLKPNEFYIHAIPRAERRRAIPLLGPRAGPTSAPVNGTNGIADEGGHPMYVYYGSNMGTCEAFAQRIAGDAGRYGFSAAVASLDSATENLPTDGPAVVITASYEGQPPDNAAHFVEWLGALGDADSPLAGVAYAVFGCGNHDWVQTYQRVPTRVDEGLAAAGAERLLPRGEGDAGAGDFFEAFTRWEAALWEALGKKYETAKGSGKEAGVQIKVTNATVSRADALRQADTMMGTVIENRVLTAPGAPEKRHLDIRLPEGTTYNAGDYLAILPTNPSRDVRRALARFGLLPDQEITIESASPTSLPTGRPISAHTLLSGYVELAQPATTRDLRLLSEAATSDAEKLVFQKLADNYAEEVLAARLSVLDILEAHPDVNIPLGAFLQLLPTMRVRQYSISSSPLADPTQASLTIRVFEAPCTAGRKAPLLGVASTYLGGLHAGDRVALAVRPCKTAFRLPADPALPLVMVCAGAGLAPMRGFLQERAAQKRAGRDVAKSLLFFGCRDPAEDYLYRDGDLAEWTALGIVDVRAAFSRARDQSLGCKYVQDRLWHDRADVMAAWDKGAKLYLCGSAKMAAGVKDKLVLVVQDAMQLEHAAAVEKFNMMMAGRFATDVFE

 

>CYP505D3 ug.73.15.1 Phanerochaete chrysosporium
MSQPIPMPPSVPFLGHVTTIDAELPVMSFRLLAKQYGEIYELNMLGRCILWMLVINTQELLHEVSDEKRFRKIVSGGLNEVRNAAGDGLFTAHADKEQNWAIAHRILMPSFSAMNMRNMFDDMVDVVSQLVLKWERFGPYHKINPADDFTALTLEAISFCAMSYRWVIFYSIYVRNDVHPFARAMSDFLLESGARARRPGIIAPFMRSANAKYQQDIDVLMNFVDEIIADRRAHPTDKKDILNVMLHAKDKETGLGMTEDNIRRNLLTFLIAGHETTSGMLTFIMYYLLKHPEAMRKLREEVDTVIGERPMTVDDVNKLPYLIAVMREALRLGPPASARGASPYEDTTIGGGRFAVPKDTFIMCSLYNIHRDTKVWGEDAEEFRPERMLDGKFEAMPPDSWQPFGYGMRGCIGRPFAWQEAQIALVYLMQRFTFAMADPGYDLRLKQTLTIKPHEFFIHAIPRADRAHGAPLFSTPSPLRPRAASSAQPPADTAGRTPVYVLYGSNTGTSEGFAQRIASAAAGKGMYSRSTIGTLDSAAAHLPTDGPVVIVTASYEGQPADNAAHFVEWLSSLQGTELEGVRHAVFGCGNRDWQATYQRVPTLVDDALTARGSIPLVLRGAGDAAASDFFEAFEKWETGLWGALREAYGVATGANAESGISIETLDTGKGRASILRQPDAALGTVVENRVLTAPGAPEKRHIEFKLPEGMTYQTGDYLAILPVNPQRDVHRALARFGLLPDQEITIRSAGPTTLPTDRPVNVSTLLSGYVELGQPATTRDLRLLSEHAKSDSTKAALQALLDNYASDVLGARLSVLDILEAHADIALPFAAFLDTLPSMRVRQYSISSSPLADAAHASLTISVLAAPARSGRPERFLGVASTFLGGLRAGDRVPLAVRPSAAAFHPPADPSVPLLLVGAGAGLAPLRGFLQERALQKKAGRDVAKSILFFGCRRPDEDLLYGDAELKEWQELGVVDVRPAFSRAPEHSFGCKYVQDRVWHDRAEAVATFKAGAKLYICGSSRMAAGVKEQIVLIVQEDSKLEYPEAVEKFEKIMVGRFATDVFE
 
>CYP505D4 pc.73.11.1 (ug.73.16.1) Phanerochaete chrysosporium
MTHPIPTPPTVPLLGHATLIDHDFPMGTNALWAREYGEIFRMCFPGRTVYVVSSYELVHEASNDKLFRKSVGGPLAELRSSVGDGLVTANVPGEENWGIAHRVLMPCFSTISLRNMFDDMVDVVSQLVLKWERFGPHYRIDPAEDFTALTFEAISLCSMSYRMNPFYNSAMHPFAAAVVDFQVECMARSRRGKLLNALIRSAKTKFEQDRDLLMQYADETVLEDRKAHPIEKKDVLWTMINRADPVTGKKMTDLSVKQNLLTLLMAGHETTSGMLTFAMYHLLKNPEAMRKLREEVDTIIGDRAMTADDLSRLPYLVAVMRETLRLSPSAPARIVQAMEATTLGGGKYAIAKDDTLLIATYVSQRDPAIWGPDAEEFRPERMLDGKFEALPPDAWQPFGAGIRSCIGRPFAWQEVQIVLVSLMQRFTFAFADGHYDLRMKQTLTMKPHDFYIHAIPRTDRARVPPLLGVRAAPAQSTDGEKGKVEAGEGAPPMYVYFGSNMGTAESFAQRIAGDAGRHGFKATVAPLDAAVEKLASDGPVVVITASYEGKPPDNAGHFVEWLSNLGDESALAGVSFAVFGCGNRDWARTFQRIPTLVDDALGAHGGARIIPRGVGDASTGSFFESFANWEEGLWAALAEKYETAKPTSVGGLELVVTDAGPGRADALRQPDTTMGTVVENRVLTAPGAPVKRHIEIQLPEGTSYTAGDYLAVLPTNPPRDVRRVLKRFALLADQEITIQSADPTSLPTGRPVNVYALLSGYVELAQPATTRDLRLLIEASSTDAEKQVFKELADNHAERVLKPRLSVLDIVEAHPSVHVPFAAFLQLLPAMRVRQYSISSSPLVDPARATLTIRVFELPGAPARRPHLGVGSTFLARLAPGDRVQLAVRPCKPAFRLPADPTVPLVLCCAGAGLAPMRGFLQERAMQKQAGRDVGKSLLFFGCRDPQEDYLYKDDDLKAWVDLGIVDVRVAFSRAPDQSLGCKYVQDRIWHDRADVLAAWNQGAKLYLCGSAKMATGVKDKLVHVVRDATGVDEASASDKFNEMMDRFRYRYFRVRYEHVVSAAFTYCIWNYLRDHSTRFNCPIAMACTFRLGAIARGSICATWLRWREE
 
>CYP505D5 pc.73.14.1 Phanerochaete chrysosporium
MTTPIPSPPSIPFLGHVTIIDREVAIYSYNLLAKQYGEIYQLNMMGALRIIVICSQELLHEVSDEKRFRKIPRSALEQVRNAVGDGLFTANGDDPNWHLAHRILMPAFSTMNTRNMFDDMVDVVNQLVQKWERFGPRHKIDPAQDFTALTFEAITFCAMSYRELTLPQEGVHPFARAMADFLVESGNRALRPGIVQPFMRSTNSKYEEDIKIMEHYVNDIYEQRKANPTDKKDILNLMMYGKDTQTGEGLSEKTIKDNLLTFLIAGHETTSGMLTFIIYYLLKNPEAMRKLREEVDTIIGSRPMTVDDVHKLPYLIAVMREALRLGPPAPMRGAASFEDTLLKGKYPVAKDVPIYCGVYMVHRDPKVWGEDAEEFRPERMLDGRFEALPPEAWQPFGFGVRACIGRPFAWQEAQITVVYLMQRFTFVMHDPSYDLQLKQTLTIKPHEFFIHAIPRTDRPSIVPIPTPSSTLLRDQTAPAAQPPVTTPGEGGGHRMYVLYGSNTGTCEAFAQRVASDATVHGEVSVFIGTLDSAAGHLPSDGPVVVVTASFEGQPADNAAHFVSWLTALNGSALADVSFAVFGCGNRDWASTYQRIPTLCDDTMAARGGKRLVPRGEGDAGSSDLFESFEHWEAGLWEALQKTYGTTKVEGRQEAIKVSTVDAGTARATALRQPDTMLGTVVENRLLTSPGVAEKHHIEFQLPDGLTYRTGDYLAILPMNPSRDVQRVLAHFSLLPDQEVTISAAGPSPLPTGRPVNVSSLLSGYVELSQAATTRDLRILMSAAKSEDTKAALSELLDGYAEKMQAARLSVLDILEAHPGLDISFALFLQLLPSMRVRQYSISSSPLADPTRASLTVSVLSAAPTAGRREPFLGVASTYLASLRAGDCVQLAVRPSAAAFHPPADPAVPLVLFCAGAGLAPMRGFLQERALQKQAGRDVAKSILFFGCRSPQHDFLYADSDLRTWTELGVVDVRPAFSRDTEHSAGCKYVQDRVWADREDVVKVWKAGAKMYVCGSGRMATAVKQKLVEIIAAQLNVDSEKATETFNNIIKGRFATDVFE
 
>CYP505D6  pc.17.40.1 Phanerochaete chrysosporium
MTSTIPTPPSIPFLGHVASIEREVPLRSFRLLSEQYGEIYELNILGRKLLVVSSAKLMSDVSDDKKFYKNMSGPLMQVRNAVGDGLFTAYGEEPNWGIAHRLLMPAFGTASIRDMFPDMLDLASQLVLKWERFGPKHRIDPAEDFTRLTLDTIALCAMSYRLNSFYRDSSHPFVQSMVDFLVECNLRANRPGLLTSVMVQTNAKYEEDIKTMTELADEIIAERRRNPTDKKDLLNIMLYSKDPKTGQSLSDVNIRNNLLTFLIAGHETTSGLLTFALYYLIKNPEAMRKAHEEVDEVLGDQQIQLTDIGKLKYIDAVLRETMRLSPTAPMRTVRPFEDITIGDGKYFVPKDYTVVINTIVAQRDPTVWGEDSNEFHPERMLDGKFEALPPNAWQPFGFGMRACIGRPFAWQEAIIALAVLLQKFDFVLDDPSYELELKQSLTIKPAHFYVHALPREGKPQLLATPSAAPFSSHARETTNASLPASPGTEAKQPMYVLYGSNTGTSESFAQRIANGAAAHGFRATLGTLDSVADHLPTDGPIVIVCASFEGEPADNAAHFVERLTSLQDKPLQNLRFAVFGCGHHDWFRTYQRIPKLIDQTLEDRGAQRLVPRGEGDAGSSEFFEAFEAWETKLWEVLPEEYNTVVKQDITSGLKVETVGEGATRAVDLRQHDAALGTVIENRVLTAPGAPQKRHIEFELPEGVTSRAGDYLAILPSNPPQDVHRVLARFGMLPEQQIVISSSGPSSLPTGRQISAFDLLSGYVELSQPATARDVRTLLNIDSSDATKESLKALLESYSDAVLGRRLSVLDLLEQYPDIKLPFAAYLALLPSMRIRQYSISSSPLWNAQRVTLTVSVLEAPALSGRKEPFLGVASTYLANLRPGDKVQMAVRASNAAFHLPQDPRTPLVLFAAGSGLAPMRGFLQERALQKKAGREVGRAVLFFGCRRPDEDYLYSDSDLKEWEELGVVELRPAFSRAPEKSEGCKYVQDRVWHDRRALDGLYEAGAKWFVCGSGKVARGVKEVLTAMIKESRGYSDEEAAAAFERATVGRFATDIFE

 

>CYP505D7 gx.187.5.1 Phanerochaete chrysosporium
ATPIPSPPSVPFLGHVTIIDREVAIYSYNLLAKQYGEIYQLNMMGAKVVVICSQELLHEVSDEKRFRKVPSSALDQVGNAAGEGLFTAHGDNPNWHLAHRILMPAFSTMNTRNMFDDMVDVVNQLVQKWERFGPRYKIDPSQDFTALTLEAITFCAMSYRYGRIXVHPFARAMADFLVESGNRALRPGIVQPLMRATNSKYEENIKIMQKYVDDVYNQRKENPTDKKDILNLMMYGKDPKTGERLSEKTIKENLLTFLIAGHETTSGMLTFILYYLLKNPEAMRKLREEVDTMIGSRTMTVDDVHKLPYLIAVMRETLRLGPPAPARGTAPFEDTLLKGKYPVAKDGRIYCGIYMVHRDPKVWGEDAEEFRPERMLDGRFEALPPEAWQPFGFGVRACIGRPFAWQEAQITVVYLMQRFTFVMHDPSYDLQLKQTLTIKPHEFFIHAIPRTDRPSIVPIPTPSSTLLRDQTAPTAQPGPVTTPGEGGGHRMYVLYGSNTGTCEAFAQRVASDATVHGFKAVIGTLDSAAGHLPSDGPVVVVTASFEGQPADNAAHFVSWLTALNGSALADVSFAVFGCGNRDWASTYQRIPTLCDDTMAARGGKRLVHRGEGDAGSSDLFESFE

 

>CYP505E1 Aspergillus niger

fgenesh1_pg.C_scaffold_3000014|Aspni1

77% to CYP505E2, 48% to CYP505C3

revised 3/23/2009

MRDAERIPGPT (1)

PLPVVGNLFDIDLEHVLQSVIGLANKY (1)

GPLFQITINGEKQIFATSQALVDELCDESRFHKAVASGLEN

LRMLAHDGLFTAYHGERGWGIAHRILVPAFGPLRIQSMFDDMGDLAQQLCLKW

ARQGASNSINITDDFTRLTLDTIALCTMDFRLNSFYNNDTMHPFVESMLYVLREADVQSALPGIANSVRIMAHRRMLKNIEA

MRTIARDIIHDRRKKENPADDLLNTLLNGRDPVTGEGMSDESIIDNVITFLVAGHETTSGLLSFTFYYLVQHPDILKKAQ

KEVDETVGQAQISVQHLAELPYIDAILKESLRMMPTAPGFTVTPKKAETLGGKWLLNAGQPINVLLPACLRDRSIFGPNA

DEFSPERMLAENFSKLPPNSWKPFGNGERSCIGRAFAWQEAQLVVAMILQNFDLVPDDPSYTLRIKETLTIKPDGFRVRA

TLRHRQTATGLFQHTLSARNDTSLASSSAHFIKKSEDQAPAGGRPICFFYGSNSGTCKALAHRLASDLMPYGFTDQKLAV

LDTAVDNLPRDQPVIILTTTYDGQPTDDAKKFVAWLESGKVPALQGISYAVFGCGHHDWTQTFYRIPTLIDELMHKAGAT

RLAPRGTANAAVSDLFSDLEAWEETSLLPALRETFLLSSSSDLEPRNLHQLQISLSKPRRIDLHKDLMEARVTTVRILTN

PDTPEKRHIEFRFQGDTTLRPGDHVNVLPVNPPSTVLRVLAQFNLAPDYSITINSFNTLGLPQATPVSASELFSAYVELS

QPATRN (0)

NLRILAATAQSDEDKQELIHLQ

DSYDSLVRDKRVSVLDLLEQFPSVSLPIAAFISMLPALRLRTYSLSLAPSFKPSHGSLTFSVVNEPARNGNRRY

LGVGSNYLASLTPGSILYLSPRPAKEAFHLPVDQSRIPIIMICAGSGLAPFLSFIQDRMIWQQQDKPLARALLFFGCGGR

FLDDLYHEELSEFEAAGVVDVRRAYSKVLDYDMARGCKYVQDRLVAEANAIRHLWAQDATIYVCGSADMAKGVEGVLEKL

LGMLPRERYVTEIYQMQTRDNVSEWLI*

 

>CYP505E2 Neosartorya fischeri

~77% to CYP505E1

NFIA_004540

Note: this seq does not have an ortholog in A. fumigatus

one stop codon

MKDTERIPGPK (1)

PLPVVGNLFDIDFEHGLESIIEMANNF (1)

GPLFQITINGEKQIFATSQALVDELCDESRFHKAVVAGVQNLRMLVNDGLFTAYHGERGWGIAHRILVPAFGPLR

IRNMFEDMSDIAQQLCFKW ARQGGSTSINITDDFTRLTLDTIALCSMGFRLNSFYNSETMHPFVQYMVDVLREADVQAML

PGIANSLRVTAQRRMSKNIEAMRSIAREIIQGRRDKGESVDDLLNTLLSGRDPVTGEGMTDDSIIDNVITFLIAGHETTS

GLLSFTFYYLLQNPHILEQARNEVDDVVGVD*ITVQHLAK

LPYIDAILKETLRLMPTAPAFAVFPKKPEVLGGKWTINARQAVIVLLPACLRDQAVF

GPDAGEFRPERMLEENFSQLPPNSWKPFGNGERSCIGRAFAWQEAQLVVAMILQTFDLVGNDPSYKLRIKETLTIKPDGF

RLRATLRRGQSATGLSRRSMSVSADGTSAASSTHLAGKCKGLEPAGGQPVSFFYGSNSGTCKALAHRLASNMMARGFTNQ

KLAPLDSAVDNLPRDQPVIIVTTTYDGQPTDDAKKFIAWLESGKVPALQGVSYAVFGCGHQDWTKTFYRVPILIDDMMHK

AGATRLAARGAANAAVSDLFSDLEKCEETSLLPALRETFLPSSSSDVELLNPQQLQISLSKPRRVGMHQGLVEVKVTAVR

ILTSPGTLEKQHVEFRIQGETTLRPGDHLNILPANPPSTVSRVLAHFDLAPDYSITINSFNTLGLPHATPVLAAELFSSY

VELSQPATRNNLKALAATALSNADKQELIRFHDSNDPLVRDKRVSVSDLLEQFPSISLPLAAFISMLPALRVRTYSLSMA

PSFKPSHGSLTFSVVNEPAWSGNGQYLGVGSNYLASLTLGSILYISPRPAKDAFHLPMDQSSEPIIMICAGTGLAPVLSF

IQERMVWLQQGKPLAKALLFFGCRGPHLDDLYHEELSEFESAGVVEVRRAYSKAPDHDLAKGSRYVQHRLVTEAEAIQDL

WAQNAKVYVCGSGNLAKGVQTALENMLGALPQE*

 

>CYP505E3 Aspergillus terreus NIH2624

AAJN01000001.1

79% to CYP505E2

ATEG_00065.1

        MIKETEQIPGPR (1)

        PLPVVGNLFDMDLEHGLECLIRLADDF (1)

204332  GPLFQITINGEKQIFATSQALVDELCDESRFHKAVMGGLEKLRMLASDGLFTAYHGER  204505

204506  GWGIAHRILVPAFGPLRIRNMFEEMNDVAQQLCLKW ()

        ARQGSSTSINITDDFTRLTLDTIALCTMNFRLNSFYNNETMHPFVKSMLYVLRESDI  204865

204866  QSMLPGIANCIRVKARSRMSKHIQLMRNMARGIIQERRDQAEPVDDLLNTLLNGRDPVTG  205045

205046  EGMSDDLIINNVITFLIAGHETTSGLLSFTFYYLLQNPHILERAQNEVDEVTGGERITVQHLGR

        LTYIDAILKESLRLMPTAPAFTVTPKKPEVLGGAWAIDAGQAVNVLLPVCLRDRSV  205405

205406  FGPDADEFRPERMLEENFSKLPPNSWKPFGNGERSCIGRAFAWQEAQLVVAMVLQTFDLV  205585

205586  PDDPSYKLRIKETLTIKPDGFRVRATLRRGQSATGLSQGSMSASGATSSVASPGPPAATG  205765

205766  AQSNPAGGQRISFFYGSNSGTCKALAHRLASSLMGRGFTEQKLAALDTVVGNLPTDQPVI  205945

205946  IVTTSYDGRPTDDAEEFVRWLESKRPVLQGVSYAVFGCGHHDWAKTFYRIPILIDDLMH  206122

206123  KAGATRLTALGTANAAVSDLFSDLELWEETNLLPALREAFPPSNSSDVESSEPHQLQICV  206302

206303  SKPRRVDMHRGLVEAKVTAVRTLTSPDSPEKRHVEFHVQGDTTWRPGDHVNILPVNPLST  206482

206483  VSRVLAYFQLAPDHSITVNSFNTQGLPSATPVSATELFSSFVELSQPATR () 206632

206695  QNLKALAMAAESKTDEQELIRLHDSYDALVRDKRVSVLDILERFPSISLPIGIFISMLPP  206874

206875  LRLRTYSLSMAPSFKPSHGSLTFSVINEPAWSGNGQYLGVGSNYLASLTPGSLLYLSPRP  207054

207055  AKDAFHLPADQFNTPIIMICAGSGLAPFMGFIQERMTWLKQGRPLAKGLLFFGCRGPHLD  207234

        DLYYEELSEFEDAGVVEVHRAYSRAPDDVRAKGCRHVQHRLVTEAEAVRDHWGRNAIVYV

207415  CGSSNMARGVQTVLEEILGTLPPERYVAEIF*  207510

 

>CYP505F1 estExt_fgenesh1_pm.C_chr_30459 [Mycgr3:99701] Mycosphaerella graminicola

JGI model Mycgr3/chr_3:2874198-2877434

47% to CYP505A1   AB030037 Fusarium oxysporum

49% to CYP505A2   N. crassa, 52% to 505A13 Aspergillus fumigatus

52% to CYP505G1 in this genome

MVSTKTVPRPWGLPLIGNVPSIDMGYPLGSLNSLADRYGEIYQLNLIGKEALFISTHKLFAEVCDETRFQ

KHLLAPLVQLRNVVGDGLFTAFPGETNWEIAHRTLMPAFGPLPIQSMFAEQHDIASQLVLKWARFGPDHD

IAVTDDFTRLTLDTIPLCSMDMRFNSFYHDDLHPFVDAMVGVLAAAGDRARRPDVADYVLRAQRAKFDKD

IAYLRKLSTELVARRREKGTQKKDLLNALLNNKDPKLGVGMTDDSIVDNMVTFLVAGHETTSGLLSFLFY

ELTSSPEAYKKAQQEVDEVVGSGAVNVGHLSKLPYLTACLRETLRLHSTAPAISVTALKDDIIGGQYAVK

KGQGINCLLAKIQSDPAVWGTDASEFKPERMLDEIFNKLPAHAWKPFGNGVRGCIGRAFAWQEALLTVAL

LLQIFNFRKTDPSYKLEVAMTLTIKPKNFTMRATMRDPSILERLGAVSVSGEVSSAKATTQHARTSSLAA

VESAGTTINILYGSNTGTCESLASSLGSTAKSRGYKAYVDTLDNGMSSISADKPTVIITASYEGQPPDNA

MKFSKWLNSDESSSLAKSKYAVFGVGNREWVTTYQRQPKLIDEVMGQKGAESLVDRGIADVCDGDIFNAF

DRWTDEKLWPAVDAAFSAGKSAASSDEGLSITVDTESRVKQLRQDVQAAVVQDVKLLTAPGKPAKRHIQL

KLPPGTDYKAGDYLTVLPVNPEPSVRRAMARFKLTWDATITIDSSSQTTIPTGRPISAFDVFASYVELGQ

PATDKQISQLASTIPDEKDRQSLTNTSLKPAAKTKTPSLLELLETYPTATYTLSQFLAAVPSMRTRQYSI

SSSPLVDPGSASLTYSVLDAPANPASPADGKRFLGVASNYMARAQPGDHIQVALRPSHAGFHLPSDDSKP

VLMACAGTGLAPFRAFVEERAVKIAAGKNLGPAILFFGCNAPDEDDMYRSEFDAWTKSGAVDVRRAYTFA

PEKSEGCKFVQHRVWHDRKEMVELIQKDACMYICGAGVVGAALVDVVQRIYVEYKGCTEEEAKKWFEGLR

GERYWSDIFS*

 

>CYP505G1 estExt_fgenesh1_pm.C_chr_70227 [Mycgr3:100796] Mycosphaerella graminicola

JGI gene model Mycgr3/chr_7:1478709-1482337

52% to CYP505F1 in this genome, 46% to 505A1 Fusarium oxysporum

MTHPIPSPRAYPFIGNLLDIDASNPTASLAHLADVYGPIFKIRVPKERLWATNYAVASELFDEKRFQKAV

TGPLEQVRNLTGDGLFTAYQGEPAWQLAHRLLMPAFGPLPIKSMFP EMQDIVNQMVLKWARFGLDREIDV

AD DFTRLTLDSIAICAMGDRFNSFYHEGQHPFVDAMVGTLSGSFARSRRLPLPSSFYAKEDQAYAKDIAT

CENLAKELLTNRRANPTDKKDLLNAMINAEDPKTGERLSDPVIIRNMVTFLIAGHETTSGLLSFLFYLLL

ETPPAYRKLQEEIDTVVGKRTVTVDDMGKLPYVEACLRETLRLYSTAPAFTLTAKGDQIIDEKYLIKDGQ

HISVLLSKFHRDPEVYGPDVEAFRPERMYKDAFTNLPPNAWKPFGNGSRACIGRPFAWQEAILAVAVLVQ

TFQFTKADPNYKLEIQTALTIKPKGFFMKAKPRNADFLDTAAQLGEAPKAKVQAPGVRGKSDVDKSKLQP

LEILYGSNTGTCEALAQALASASPDHGFNATVRSLDEGVSSAKKNHPVVIFTASYEGQPPDNAGHFVEWV

TSSSNAGIKDVPYAVFGVGNREWAATYQRIPKVVDEALAKIGAKRLVDREECDVAADKVFEKCDDWQEFK

LWPALRKQFN VDAPRDAGGLKGLDLSLDTQ MRSTILRQDTLIGEVTETKLLTSPGAPRKRHIGIRLPSGT

QYRAG DYLAVLPVNPPALVKRVLNRFQLPWDAMITIAESNTTSLPKGMPISAHDTLASLVEIESPVSSRV

AASVSKSIPDEKAASELEEKLQKGNLTMSLLDLLEQYPEAQISFAQFLASLPPMRIRQYSISSSPLADPS

VATLTYSVIDAPNRSGGKQNFVGICTTYMERLSVGDRMHISLKPSRTGFHLPADDHSAIIMACAGTGLAP

FRAFVAERAIKKAHGGEVGPALLFYGLNRPEEDDMYRDEFDAWEKEGVVSVRRAYTFKPEESHGTKFVQE

RLWHDREDVVDLFRQDAKLYLCGAGVVGQGVEGVMTRIRSEMTGENEETAKQWVQDLKGDRFWSDVFS*

 

>CYP505G2P Fusarium verticillioides

53% to CYP505G1, pseudogene, possible orthoog of CYP505G1 Fusarium oxysporum

FVEG_11770

MSKQIPNPKGYPFIGNVLDVNPDHPQESLAQISESYGPIFRLYLPAERIFVANY

ALAKDLFDEARFEKAVIGPLEQVRNATKDGLFTAYPGEHNWEVAHR

TLMPAFGPLSIQNMFG (small deletion here about 26 aa)

DFTKLTLDSIAL (2)

CAMGERFNSFYHDDNHPFVGAMNNMLVESFARSRRPAFASAVMKASNA

RYESDIAELENISRELLEARRQNP

VDKKDLLNAMINGRDPKTGEGLGDDAIIRNMITFLIAGHETTSGLLSFLF

YLLLETPSALRRAVEEVDRVLGSQLITVAHLAKLPYIDACLKETLRLYPT

APGFTLRAKGDQVLNNEYTIKDKECVTILLARLHRDPDVYGPDAEDFDP

SRMLPDKFNKLPANTWK (0)

PFGHGVRACIGRPFAIQEAILATATILQTFHITKDNPSYQLQIRTA

LTIKPQGFKIRAKLRNPSFLEGAELGHGSGSGNAASSKTPSKAHAAKQE

GNSEPISIFFGSNTGTCEALAQSVASSAEENGYAPKVDSLDNAISSLKPNV

PVVVITGSYEGQPPDNAAHFVEWLASLSSGELSKVPYAVFGVGN (1)

EEWHNTYQRIPSLIDEQLEKAGATRLVSRAAIDVAKTNCLETIEEWQESELW

PALQKNSGKSSRTASGGARELKLDY&VLG &

LRQSLLRQDVMTAQVSRDAAADQAWAA*ESVTWYRLPSETT*PGCDYLAVLPLN

HPDVVHRVMRRFHLPWDTTVCIDGSKSTSLPTGKPFSVQNLLAGMLELGQPVTE

RQALQLTETIPDKTKSDELKERVKQGNLSKLNTTLLDLLEDYPSAT

YSFGQYLAALPALHLRQYSISSSPVASASPSECSLTYSVIDAPAKGNSQGHRYLG

ASTYLERLQPGDHIQVGVRPSRSGFHLPAESKTPIVMACAGTGL

APFYGFVAERAVKKSQGLTVGTAQLFYGCNGPDEDDLYRGQFDEWERDGVVKVYRA

YTFAPEKSENCKFVQDRMWFDRQDIVTSFREGAQFYICGAGIVG

SSIQKVLAKIRAEAADCDQESASVWLDSMKGERYWADVFA

 

>CYP505H1 Fusarium oxysporum

46% to CYP505B2

FOXG_15285

MAVKQESKKHDEKHKGQIPGPKGLPIIGNLLDIDVTNSLQSIIDMAKDYPKLFALNVGGNTEIMICSRELMDELSDEARF

HKLVVGGVEKLRPLAGDGLFSAQHNNQEWAIAHRILMPLFGPLTIREMF PDMRDISEQLCLKWARAGPSATIDV GNDFTR

LTLDTIALCTMGFRFNSFYSNDKMHPFVESMVAALVDAEKQSMLPDVVQSFRLRAQSHFKKHAAVMKSTCHEILEQRRKN

PVEGKDLLNAMMNGKDPKTGMGMSDGNIVDNLITFLIAGHETTSGLLSFAFYYLLENPEKLQKAREEVDEVLGDENLTAD

HLPKMPYINMIFRETLRLMPTAPGFYVTPFKDEVIGGQYNVSAGDPLFLFLHMIHRDPEVWGPDAEEFRP

ERMADEQFNK

LPKNAWKPFGNGMRGCIG REFAWQEAQVVTIMLLQNFDMVKADPNYKLKIKQS

LTIKPDGFNMKVKLREGRDLTNLFKNP

SLASSKQPSLSSRMNLKISQKDLKPISIFYGSNTGTCEALAERLSADCATFGFMPSKPLPLDEATRNLSKDGPNIILAAS

YDGKPSDNATEFTKWAESLQPGELDGIQFAVFGCGH KDWVSTLYRIPKILDKCLAAAGADRLVDIGLTDASTGRLYPDFD

DWAHSKLFPELASRHGITLDHAPDSLELSVTINQSQRNDIGGNFKRAEVVENTLLTSPGVPRKHSLLLKLPKDMAYTPGD

HVL

VLPKNPPQLVDRVMTCFGVDDDTVLTVSSQRPTFLPTGTPILASNLFSTLVELSQTVSRTSLKRLVDFAGNDGTKGE

IQSLAGDRYDAEIDQQRMSILDILRKYPSINMPLSTFLSMLPQMRPRTYSFASTPEWKAGHGSLLFSVVEATEASGSSLA

RPGGLATNYMAQLRPGDSILVEPRPCRPELRTAMITELNVPIVMIAVGAGLAPFLGFMQKRYLQTQNSGHLPNSPCTLFF

GCRGAKMDDICRDTLDEYSRAGVVSIHRAFSRDWDSPYKYVQHLVAKHADTLARLWGQGAIVMICSGKKVSDDVFDVLGP

ILHSEDRRLNTTRASDWKTWREEVSKERLILEVFG*

 

>CYP505H1 Fusarium verticillioides

94% to CYP505H1 Fusarium oxysporum = ortholog

FVEG_12598

MAAKQKSKKHDEKHKGQIPGPKGLPVIGNLLDIDVTNSLQSIIDMAKDY

PKLFALNVGGNTEIMICSRDLMDELADESRFHKLVVGGVEKLRPLAGDGLF

SAQHNNQEWAIAHRILMPLFGPLTIREMLPDMRDISEQLCLKWARLGSSA

TIDVANDFTRLTLDTIALCTMGFRFNSFYSNEKMHPFVESMVAALVDADK

QSMLPDVVQSFRLRAQSHFKKNAAVMKTTCHDILDQRRKNPVEGKDLLNA

MMSGKDPKTGMGMSDGNIVDNLITFLVAGHETTSGLLSFAFYYLLENPEK

LQKAREEVDEVLGDDAITADHLPKMPYINMIFRETLRLMPTAPGFYVTPF

KDEVIGGQYNVSAGDPLFLFLHMIHRDPEVWGPDAEEFRPERMADEHFNK

LPKNAWKPFGNGMRGCIGREFAWQEAQIVTIMLLQNFDMVKADPNYQLK

IKQSLTIKPDGFNMKVKLREGRDLTNLFKNPSMASSKQPSLSSRKNLNISQ

KDLKPISIFYGSNTGTCEALAERLSADCATFGFMPSKPLPLDDATRNMS

KEGPNIILAASYDGKPSDNATEFMKWAEALQPGELEGTQFAVFGCGHKDWV

STLYRIPKVLDKCLADAGADRLVDIGLTDASTGRLYPDFDDWAHSKLFP

ELASRHGITLDHEPDSLELSVTVNQSQRNDIGGNFQRAEVVENTLLTSPGV

PRKYSLLLKLPKDMAYTPGDHVLILPKNPPQLVERVMTCFGVDDDTVLTV

SSQRPTFLPTGTPILASSLFSTLVELSQTVSRTSLKRLVDFAGNEETKAE

IQSLAGDRYDVEIDEQRMSILDILRKYPSINMPLSTFLSMLPQMRPRTYS

FASTPEWKPGHGSLLFSVVEATEASGSSLARPGGLATNYMAQLRPGDSVL

VEPRPCRVELRTAMTTELNVPIVMIAVGAGLAPFLGFMQKRYLQKNSGQL

SNSSSTLFFGCRGAKMDDICRDTLDEYSRAGVVTIHRAFSRDWDSPHKYV

QHLVAKHADTLARLWGQGAIVMVCSGKKVSDDVFDVLGPILHSEDRRLNT

TRASDWKTWREGVSKERLILEVFG*

 

>CYP505J1 Aspergillus clavatus

46% to CYP505A15

ACLA_032390

MSEIPQPPGLPYIGSLLEIDPKYTVKSLRRLGDIHGPIYRLSIVNITRIVVSNHELFDELCDESRFVKSVSGPLSQMRNL

VHDSLFTAHPNEQNWGIAHRIIVPAFGPVPIQDMFHGIYDIASQLILKWARQGPSKPINVQEDCTRFTLDAVTLCAMGVR

LNSLYSEEKPPFAQAMDGFMRESNYRAQRPAFATRLLWWYRQQYDRDIDFIRKFAKELINARRKNPTENQDILNVLIKGR

DPETGRQMSEESIIDNMVTFLIAGHETTSGGIAFLLYYLVKSPDVWQKVQNEVDAVVGSGRITSKHLSKLRYITACVRES

LRLWPTIPVIAFKPKDRQNPTVIGKDKYSLMPGQTIIALLPAIHRDPDVWGEDADEFRPERMVDEELSALPKNAWKAFGN

GARSCIGRPFAMTEMVLITALLAQNFDVEQHDPDYELDIDQIVTLRLRNFFLRVKPRPGIDAVQLANRLFNDQEAKSRPG

PEATMTPAVRDKLKPITVLYGSNTGTCESLARQFSRDASRYGFNPTMLPLDSALEKLPTDWPVIILAASYEGQPAANAAK

FVSWLQKIKKPILSGVSFAVFGCGNRDWTATFHRIPKLIDRLLSDAGAHRLLELGLADVSSMVVQETFETWYGNQLWPAL

RAKYTISELRPLPSKLTVEFTNHLRQATNGQEFIEASVIENKLLTASGVKAKRHLELALPPNAEYTVGDYLHILPMNPPV

TVQRAMGFFRLAEGTYMKVTGGLSTVLPIGKEISVEEALSRYVELSQPATQKILERISLSTPVLTEREKLSHQAASSTSL

QDKRISILDLLEHAPSSTISFADFISMLQPLRARPYSISSSPLNCARSCSLTVSIVDNPEPTARETRVLGISTHYITRLK

PDQRILCSIRPSNSGFTFPDNPETTPMVMICVGSGIAPFRAFVQERVCRAEKGQKLAPGLLFIGCKSPKHDRLYGSELDQ

LEEAGIVTVRYAYSGLPYRKHVQDKLWDERETLVDMYKGEARFYLCGPVELRDSVLAVARRIYMATVEANGEVRGEDEYQ

NWLAKMNKGRFAADTFA*

 

>CYP505K1 Mycosphaerella fijiensis

48% to CYP505A2   N. crassa

e_gw1.1.1150.1

MSKSPRDNIPSPPGLPIVGNLLDIQDEVPIRGLENIADTYGGMFKLNIMGRERLFAANVALLDELCDESRFWKAPGDGLN

PLNQGGGGGSKGLFTAESEETMDWQLAHRILMPAFGPLAIQDMFDEMHDIASQLVLKWARLGPSYSILVTSDFTRLTLDT

IALCAMDYRFNSFYQDELHPFVQAMNTSLAFSSDRTKLGSIVKRLLPWDKSIQKLAADRQYMTSVSKELVELRRNNPTNK

RDLLNAMVLGKDPNTSEKMSDGLIASNMTTFLIAGHETTSGLLSFAFLFLLQHPDAYFKAQQEVDRVLGRGKIEVRHLQE

LKYIDAVFRETLRLCPTAPAFTRSIRKDNPNDVEELLGGKYAINRDDKVLCLLSKCQRDPEVYGEDANDFKPERMLGDAF

NKLPKSAWKPFGTGLRSCIGRAFAWQEAQLAMAMILQNFNIRLDDPGYQMRIKQTLTIKPEGLYMRATLRDGISAATLQT

ALASSPDAVRSAIEDTISRADSGMEDAKPMTILYGSNTGESLPLGSHGTCTALAQKLSLEGRRHGFDARVMELNDAVDAI

PRDGQPVVLITASYEGQPTDNAAQFVKWLEVMPPGESRFEGVNFAVFGCGHRDWTGTFHRIPKLVDELFKKHGATQTVRA

GFADAAAGDIFSQFEDWTDRSFWPSVSPSSGAAQEAPELEMDVATRERSTYLRQDVRKGIVKSSWCLTADGEPEKRHLEI

KLPDNMTYEAGDYLAILPLNPQQTVTRVMKHFKISALATTTIKPGAATFLPTGVPLSIVELLKGFVELSLPATKRDIQAC

IACTSDTTEKEELRALQSESAFRELDESHVSLLDLLERYTSIGLGFNTFIAMLQPLKPRLYSISSSPLVDATSCTVTYGV

IDEDAKSGNGRYVGVTGSYLSGLMTGDEILVSVRATNRYFHLPAEISQTPILMFGAGTGIAPFRGFIQERAQQIAAGRSL

APAIMYMGCRSSSSDRLYSDEMDKWVKHGVVDIRYAFSQESHASEGCKYIQDRMWKEREDVIRLWRDGAKVFVCGGPAVC

EGLGDVSRKLLLESMKSRGQEMSEKEAELWFRERRNVRYVVDVFA*

 

>CYP505L1 Mycosphaerella fijiensis

46% to CYP505A2   N. crassa

46% to CYP505K1 Mycosphaerella fijiensis

estExt_fgenesh1_pg.C_180062

MASNVPQPKAWPIIGNVLDVDTVNMTQSFERLADKYGEIYKLDLLGKPTYVISSVALVAEVCDEKRFCKKIDTFLNQLRL

AVGDGLFTAHNHEHNWGVAHRALMPAFGPLPIQAMYDEMYDIASQLVSKWARDGADHEINVTDDMTRLTLDSIALCAMDY

RFNSFYHEEVHEFIRAMGDVLLESGKRAMRSKIEAWLNPSAEKKFFADIATLKKVAQQCIDRRRAGPGKKDLLDAMLNGK

DPKTGEKLSDESIINNMITFLVAGHETTSGMLSFTLFYLLKNPETLQRAQQEVDSVVGSGPIEFKHIKELKYIEAALREA

LRLCPTTVGFSVGPVPGTKGPVTLAGKYVLPPDAVIMILGTRSGRDPKVFGEDAAEFKPERMHDEHFNKLPPGAWKPFGN

GMRGCIGRPFAWQEAILALGIILQNFNLRFADPSYELKIAETLTIKPDNLLIKAQLRDGIDPIQLSKKLHGEIRTAAREE

PAKHLASSKSSGDSGQLTIFFGSSAGTCEGLAQSLAASARVRGFDTVVKSLDAAVDRFPKDHPVIIITCTYEGQPPDNAK

IFVEWLKTADRSKFEGAQVTIFGCGHRDWVSTYQKMPRTIEGELTAKGAALLAPRGETDTSQGTILDDFDAWTSRLWPSI

SADGKEYSSASSLELRLSTNSRASHLHHKVWDAKVVSNRRITSLEAPEKRHVTISLPRDMQYDAGDYLAVLPLNNQQLVS

RVLRRFSLPWDTAMTITSGSHATIPENRELPVALILSGYIELGARASRKSRDVLSEHSKLSLADERGSIIDILERHPDIS

IPFGTFLELHMPLRLRQYSISSSPLADPSLATITFSVIESDEHIGTATSYLKSLEPGSTLQVAVKKSSAAFHLPLDESIP

VIMVAAGTGLAPFRGFLQERAVKAEAGRQLGEALLFVGCRDPNQDKIFAEELAQWENLGVVKVRYAYSRASEQSDGCRYV

QDRVYKEAAEIARLFDLGARIYICGSGAMGRSVSDVAVRLLVEEAEDKGEKLSEEDARLRWEKWRGERYALDVFD*

 

>CYP506A1 Fusarium oxysporum

93% to CYP506A1P rev Gibberella moniliformis

FOXG_10952 revised

MSAKLPFQLSTGAMYKHPQFPSLIA

AGVMTFTSILLVALKFWSSSATPKVKILLQDEIKSARHRALEYCFNPRAVMAKGYAK (0)

FKNEVFGLDTQD (1)

GVKLVIPPNFLDELKSHPALSFKVSIDN (0)

DMQIEYTHFGGPAEYVIHAIKANLTGSL (1)

LHDRVLRLIATNNARVFQGTAASLDEEWLEASTGYVLACFDCIRALKQWH

PWLRPLVYILIPERAAIKDQWTKGRKRVMASMRERQEKGGNLEDPPTMLDHLSNGRNEHIADDVELQLLHQMTLIAVGTV

TTFSSTTQAIYDLVAHPEYIPILREEVESVPRDPNGNFTKDSTVAMNKLDSFLKESQRFNSPDL (1)

TTFQRAAIADMKLPDGTFVPKG

TKLEINTCSIHKDHELYGNPELFDGLRFHKWRKAPGKGKKYMYSSSGTDDLSWGFGRHACPGRYLSAINIKLIMAELLMN

YDIKLPDGVSRPKNIEFEVLASLNACANA*

 

>CYP506A1 Fusarium oxysporum fragment X82490

MAPMLRPLVYRFIPERARIKDQWTKGRKRVMASMRERQEKGGNL

EDPPTMLDHLSNGRNEHIADDVELQLLHQMTLIAVGTVTTFSSTTQAIYDLVAHPEYI

TILREEVESVPRDPNGNFTKDSTVAMDKLDSFLKESQRFNSPDLSM SNLKNYKLCESL

TGHSNLPTR TIADMKLPDGTFVPKGTKLEINTCSIHKDHKLYENPEQFDGLRFHKWRK

APGKEKRYMYSSSGTDDLSWGFGRHACPGRYLSAINIKLIMAELLMNYDIKLPDGLSR

PKNIEFEVLASLNACANA

 

>CYP506A1P Gibberella moniliformis 7600

AAIM01003201.1 cont2.3201, whole genome shotgun

Note this seq has only 1 aa diff to 506A2 at 10017, but then a large gap

Like a pseudogene

77% to 506A2

also = Fusarium verticillioides FVEG_09850

10017  DMQIEYTHFGGPAEYVIHAIKANLTGSL (1)

       FHDKVLR

10311  LIATNNARVFQGTTASLDEEWLAASTGYVLACFDCIRALKQWHPYLRPLVYRFIPERAAI  10490

10491  KDQWAKGRKRVMASMRERQEKGGNLEDPPTMLDHLSNGRNEHIRDDVELQLLHQMTLI  10664

10665  AVGTVTTFSSTTQAIYDLVAHPEYIPILREEVESVPRDENGNFTKESTVAMDKLDSFLKE  10844

10845  SQRFNSPDL (1)

       TTFQRAAIADMTLPDGTFVPKGTKLEINTCSIHK  11024

11025  DHELYENPEEFNGLRFHEWRKEPGKEKKYMYSSSGTDDLSWGFGRHACPGRYLSAINI  11198

11199  KLIMAELLINYDIKLPDGVSRPENIEFEVLV  11291

       SLNEMVLGSVADVGSAPLNRISRSFSRIGIIRWNGVCLVR*

 

>CYP506A2b Fusarium graminearum AACM01000093 Gibberella zeae

MAGTTFAELHLAFYQWIDETLDHPLGRYFM

AGVGSSLVVLLVAIAAFCSRSKTPNVPILLQDEIGNARKRALEYCFNPREVMEKGYKK (0)

FKNEVFGLDTQD (1)

GLKLVIPPSYLDALKSHPALSFKASIDN (0)

DMQIEYTHFGGPPEYVIHAIKANLTGSL

VHDRVLRLIATNNARAFQGTAASEDEEWLSASTGYVLACFDCIRALKQW

HPWLRPLVYRLIPERAAIKDQWAK

GRKRVMASMKERQQKGGNIEDPPTMLDHLSNGKHEGMANDIEKQLVHQMNLIAVGTVT

TYSSTTQAIYDLATHPEYVPILRQEVESVSRDANGNFTRDSTLAMEKLDSFIKESQRF

HSPDL

TTFQRAAIADMTLPDGTFVPKGTKLEINTCSIHKDKDLYENPGDFDALRFYKMRQAPGQ

EAKYQYFS

VGREDLSWGFGRHACPGRYLSAINIKLIMAELLMNYDIKLCDGASRPPNIEFEVLVSCTTEPGTQP*

 

>CYP506-un1 pseudogene Nectria haematococca

gw1.28.129.1

probably from a CYP506 sequence, subfamily not assigned

Necha1/scaffold_28:447825-448775

35% to 506A2 mid to heme, 38% to AAIM01003201.1  Gibberella moniliformis

the top part of this seq seems to be missing

probable pseudogene, check 506A2 for TFQRAAIADMTL, may extend last exon

410941 HAGGPGESGLHAIQGHIVAS 410882

LVSRISARIFHSAASADNDHWLDIASEHVHSAVVWTENLKKWPAMLRPLVYRFVKGRGYMMQRFEEGKAL

VAQTLENKKANGGKPLSDPQSLLDYLYESGLGPDDVEAHTIAQINLCVAAIXSMAATVTQCLMDLATHPE

YAPELIEEIKTVVEKNNGVVDKRVLTELWKLDSFIKETQRLNPPDL (1)

TSFQRKALSDMTLSNGLRIPKGAR

IVLPTGAINMDREFFEDPQTFDGFRYFYRIRTANEAARNTNQMVTVGKKDLTWGYGKHACPG

RYIAEVAMKLLVVEFLMRYDIRLPENVK ERPKNIEFEGLVCCFHP*

 

MNNFIFKRLNVKEFYEAETKKLVENGHEFHLRLERT

GYVIHTCN

 

>CYP507A1 N. crassa AABX01000414.1 cont3.575 NCU09185.1 (version3)

98714 MALTELLGALPLVSSGMSKLQVAAIYLAISFVSWYIISSFTDWY

      RLKAFPGPPTTGFSNLWAARAIWTGKAHKIFPATQEKYGPITRI

      GPNALMVCDAATVVHINGVRGGYARSQDFYDCIRMDPWDHTVLS

      ESDSAMHNERKTKVYAGYHGKGEMDMEKDVDMVIAEAVELVRKK

      YMNSAATSGSKPPLDYTRIARYIAVDSVTQTGFGKAWGDVREEK

      DHFGWLGFADMIVGYLHSLAYVPAISKIVISTPLMVLLGPKPTD

      KSGIGAFLG (2) 97896

97821 LIEREVARRYKDGSAKGTPLKADTGRSSML (0) 97732

97669 DEWVKNGISQRTAVLEVSAQLPAGSDTTASALQGTMLYLLSTPS

      AYIRLKSEISTAIRTGLISSSSIITDQEARKLPYLQAVLYEAIR

      MMPPLMNGFPRQVPAGGDTICGRFVPGGTDIFVNYVGMLRDKSV

      FGEDANVFRPERYLEGDEERRNRMWKTTDLAFSHGRWRCLGQRL

      AWVQLQKVVVE (0) 97109

97044 FMRNFEMQIVDPMHPCRLRCYCTPEMDDLMVKVTEAKWD* 96925

 

>CYP509A1 AF249299 Cunninghamella elegans

MNNFIFKRLNVKEFYEAETKKLVENGHEFHLRLERTGYVIHTCN

PQAIKEILLKGDKFPKYDYNTSNNEDGSFFHEFVGFENVLMTNGSTWKKHRMIVNPAF

KTALPSRLFGDCTRDLFKLWDNEYANEPFDSDIHNITERLTLQIIGKAAFDFDFNAIL

DEKSIWKQTYDGINKAASDPLFILFPILEEKFLWLFPKRQQSFKLLNEWKKMLLSIIE

KKREAIQNNVDHGVEEAEKDLLTLMIESEHRGEGALSNEELLSDLIIFFIAGHDTTAF

ALSAAIYYLAKHPDIQEKARQEAISVLCPNGEVDEDIFPTTEDTKHFVYINQIMKETL

RINGSVLFLLSPRLVTQDVNLSGVFIPKGSQINVNIHEVHHNPNVWHDPETFDPDRWA

SNGEAEQLAGKGMAWVPFSNGNRQCIGMNFSLLEQRVILGSLLRKYEWTLPEDSIHQD

YMVNKFNLIPKPIDLKIRFKRRY

 

>CYP509B1 Rhizopus oryzae RA 99-880 (Zygomycete fungus, Mucoromycotina)

AACW02000089.1 cont3.89

P450-15 in CYP56 clan

43% to CYP509A1

RO3G_05802.1 revised

        MIFEVIQEIIQPWIESNVRILIVFGIVLAFYALVQRYNRPPAALAHLPYVSFFSFLK

        YGFKDEVYDTYAKEKLLPLLKAKG (1)

291331  GWVVRLADPVAIKQFTLKQGK (2)

        GLFASPTIQGWVSIHLNSNHEIPSIEHT (instead of the third exon above)

     (?)FPKAMSNAGEEGTLIHRF  291158

291157  IGGPNIVFLSGPDWKRHRK (0)

        IANLAFQRSMPVQLFGDLAKRML  290978

290977  RSMEKTEEDAIDVNDLFRRFTLDAIGNGGF (1)

        GFDFHAIEDKE  290807

290806  SKWVEYYESVMQGMASPFYIVFPAFDTKYVHWFRKRKQLHDNLSKLMENMDEIIEKKRKL  290627

290626  VYENGQGESKEKDLLTLMIESEMKSEGESLSNEELR (0)  290522

290469  SNLCIFFLAGHDTTANTLAAIIYELAKNKEIQEKARKEAIRILGDGPEDTAPTAEQL  290299

290298  KELDYINMIIKE (0) 290263

290192  TLRRHPPAYVTTDRVVQNDLVLGDVHIPKGSDLCLDIYSLHHNADIWSNPFEFNPERFAPGGEADS    290019

290018  QRGIAWAPFSSGGRMCVGMNFSLTEQRVLIPML (1) 289923

289869  LKKYTWSLPNDSSHQHELQKRGIAWGLVTFKDKVKIEFKKRN*  289741

 

>CYP509C1 P450-32 in CYP56 clan Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

93% to P450-33, 90% to P450-30var1, 89% to P450-31, 87% to P450-34_var1

87% to P450-29, 84% to P450-7_var1, 65% to P450-39, 59% to P450-36

56% to P450-42, 53% to P450-20a, 53% to P450-12, 40% to 509B1 =P450-15, 38% to CYP509A1

RO3G_10680.1

MEDLVTKYNMALEGALPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHLPCVSFFAMAKSLLTCEPPYNRFKRITFPAIQEGNGFYVSKIPTGWTVYVANPVAAKQLLLKSNNFPKSHYGLDTIGEKSTAVQFVGRDNVVLSNGEIWKKQRKIMNPVFHRSMPIKTVASLVPLLFSAIEEANGRIMITPKMKDFTLDALGLTIFDFDFKALQGDPDNWTSIYRLITRSIFDPISYVFCALEPLLVYVYPKRRRSVDAVAKINAKFDQVISKKREELQNGIFSNKPDNEKDLVTLMLEAGMQEDVSITNEELRHNMAVLFLAGHDSTSNTLSFCLYHLAKNKRAQQKLREEIINILGDDDIDIVPSLEELKQMKYMNMVIKETLRLGMPLDLLTPRKTVEDTFVADTFIPKDTVIAVDAGALHRDPRSWKDPDEFVPERFEDDGEQNSHEGLTWVPFSNGTRQCIGMNFSLMEQRLTLTMLLRKYEVDLPKDSIHYDHIIYEQPSYVCPESLELIFTKRY

 

>CYP509C2 P450-33 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

93% to P450-32 in CYP56 clan

RO3G_10685.1 revised

MEDLVTKYNMTLEGVLPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHFPCVSFFAMAKSLLTCEPPYNRFKRITFPAIQEGNGFYVSKIPTGWTVYVANPVAAKQLLLKSDNFPKSHRRFDTIGEKSTGVQFVGRDNVVLSNGEIWKKQRKIMNPVFHRSMPIKTVASLVPLLFSAIEEANGKIPINPRMKDFTLDALGLTIFDFDFKALQGDPDHWTSTYRLVIQSISDPISNVFSALEPLLVYVYPKRRRSVDAVAKINAKFDQVISKKREELQNGIFSNKPDNEKDLVTLMLEAGMQEDVSITNEELRHNMAVLFLAGHDSTSNTLSFCLYHLAKNKRAQQKLREEIINILGDDDIDIVPSLEELKQMKYMNMVIKENLRINTPLDLLLPRKTAEDTFLADTFIPKDTIIVIDVGALQRDPRSWKDPDEFVPERFEDDGEQNSHEGLTWVPFSNGTRQCIGMNFSLMEQRLTLTMLLRKYEVDLPKDSIHYDHIIYEQPSYVCPESLELIFTKRY

 

>CYP509C3 P450-30var1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

87% to P450-29 in the CYP56 clan

RO3G_10670.1 revised

MDDLVTKYNMTLEGVLPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHLPIVSFFAMAKSFLTCEPPYSRFKRITFPAIQEGNGFYVSKIPTGWTVYVANPVAAKQLLLKSDNFPKSHRRFDTIGEKSTGVQFVGRDNVVLSNGEIWKKQRKIMNPVFHRSMPIKTVASLVPLLFSAIEEANGRIPINPRMKDFTLDALGLTIFGFDFKALQGDPDHWTSIYRLVIQSISDPISNVFSALEPLLAYVYPKRRRSVDAVAKINAKFDQVISKKREELQNGICSNKPDNEKDLVTLMLEAGMQEDVSITNEELRHNMAALFLAGHASTSSAVSFCLYHLAKNKRVQQKLREEIINILGNGDMDIVPSLEELKQMKYMNMVIKENLRLNMVLDLLLPRKTAEDIFLAGTFIPKDTNIAVDVGALQRDPRSWKDPDEFVPERFEDDGEQNSHEGLTWVPFSNGTRQCIGMNFSLMEQRLTLTMLLRKYEVDLPKDSIHYDHIIFEKPTYSCPESLELIFTKRY

 

>CYP509C4 P450-31 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

89% to P450-30var1 in the CYP56 clan

RO3G_10674.1 revised

MDDLVTKYNMTLEGVLPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHFPCVSFFAMAKSLLTCEPPYSRFKRITFPAIQEGNGFYVSKIPTGWTIYVANPVAAKQLLLKSDNFPKSHSRIDTLGEKYMGVQFMGRDNVAISNGEVWKKQRKIMNPVFHRSMPIKTVASLVPLLFSAIEEANGRIPITPKMKDFTLDALGLTIFDFDFKALQGDPDHWTSAYRLMMQSLFHPISYVFSALEPLLVYVYPKRRRSANAVAKLNAMFDRLIEKKREELQNGICSNKPDNEKDLVTLMLEAGMQEDVSITNEELRHNMAVLFLAGHDSTSNTLSFCLYHLAKNKRAQQKLREEIINILGDDDIDIVPSLEELKQMKYINMVIKETLRLNTPLDLLTPRKTVEDIFLADTFIPKDTNIAVDVGALHRDLRNWKDPDEFIPERFEDDGEQKDHEGLTWAPFSNGTRQCIGMNFSLMEQRLVLTMLLRKYEVDLPKDSIHYDHIIFEKPTYSCPESLELIFTKRY

 

>CYP509C5 P450-34_var1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

87% to P450-32 in CYP56 clan

RO3G_10705.1 revised

MEGLVTKYNMAFEGLLPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHLPSVPFFAMAKSFLTCEPPYSRFKRITYPAIQEGNGFYVSKIPTGWTIYVANPVAAKQLLMKSNNFPKSHYGLDSIGEKSTVVQFVGHDNVALSNGEVWKKQRKVMNPVFHRSMPIKTVASLVPLFFSIIEEANGRIPINPKMKDFTLDALGLTIFDFEFKALQGDPDNWTSVYRLVTRSIFDPLSFVCNTIESLLTYVYPKKRKGAYAVVKLNAKFDQLIDKKREKLRNGIFSNKPDNEKDLVTLMLE

EDISITNEELRHHMALLFLAGDDSVSNTISFCLYNLAKNKRAQQKLREEIINILGDDDMDIVPSLEELKQMKYMNMVIKETLRLDMPLDLLLPRKTVEDMFLADTFIPKDTDIAVDAGALHRDPRSWKDPDEFVPERFEDDGEQKDHEGLTWVPFSNGTRQCIGMNFSLMEQRLVLTMLLRKYEVDVPKDSIHYDHIMYEQPTYICPESLELIFTKRY

 

>CYP509C6 P450-29 in the CYP56 clan Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

83% to P450-7_var1

RO3G_10668.1

MESLVTRYNMALEGLLPLFQKRSKSSYIVAAILLITVKQIYSFFRVPTNLRHLPIVSFFAMVKSFLTSEPPYSRFKRITFPAIQKGNGFYVSKIPVGWTVYVANPVAAKQLLLKSDNFPKSHFGLDAIGEKSTAVQFVGRDNIFLSNGEVWKKQRKIMNPVFHRSMPIKTVASLVPLLFSAIEEANGRIMITPKMKDFTLDALGLTIFDFDFKALQGDPDHWTSIYRLVTRSIFDPISYVCSAMENFLTYTYPTKRKGFNAVVKLNAKFDRLIEKKREELQNGIFSNKPDNEKDLVTLMLEAGMQEDISITNEELRHNMAALFLAGDDSISNALSLCLYHLAKNKRVQQKLREEIINILGNGDMDIVPSLEELKQMKYMNMVIKENLRLNMPVDVLLPRKTAEDIFLAGTFIPKDTIIVIDVGALHRDPRSWKDPDEFVPERFEDDGEQNSHEGLTWIPFGNGTRQCIGMNFSLMEQRLILTMLLRKYEVDLPKNSIHYDHIVYEHPTYICPESLELIFTKRY

 

>CYP509C7P P450-7_var1 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

37% to CYP509B1 Rhizopus oryzae, in the CYP56 clan

P450-7_var1 has a stop codon in the I-helix

86% to P450-33, 85% to P450-32, 84% to P450-30var1, 84% to P450-31

84% to P450-29, 83% TO P450-34_var1, 62% TO P450-39,

58% TO P450-36, 53% TO P450-20a, 55% TO P450-42, 53% TO P450-12

RO3G_04442.1 revised

MEGLVTRYNIALEGLLPLFQKRSKSSYIVAAILLITVKQVYSFFRVPTNLRHLPNVSFFAMAKSFLTCELPYSRFKRITFPAIQEGNGFYVSKIPIDWTVYVANPVAAKQLLMKSNNFPKSHRGFDIVGEKSPAAQFVGRDSVVLSNGEVWKK*RK

IMNPVFHRSMPIKTVASLVPLLFSAIEEANGRIHITPRMKDFTLDALGLTIL

DFEFKALQGDPDHWISTYRMVMLTLFDPILNVFSALERLLVYVYPKRRRSANAVAKLNAKFDRLIDKKREELQNGVFSNKPDNEKDLVTLMLEAGMQEDISITNEELRHHMSFLSLAGYDSTSNTLSFCLYHLAKNKRVQQKLREEIINILGDDDMDIVPSLEELKQMKYMNMVIKENLRLNMPVDLSLPRKTAEDTFLADTFIPKDTTISVDVGALHRDPKSWKDPDEFVPERFEADGEQKDHEGLTWVPFSNGTRQCIGMNFSLMKQRLTLTMLCKSILSPFFFCIIRVAKPYLFIYNEKV*

 

>CYP509C8 P450-39 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

66% to P450-33 in CYP56 clan

RO3G_11470.1 revised

MDVLIKKYNATFHSIISLLQKRSKASYIAAIIFLIVLKKIYSFFRVPKYLRHLPCVSYFAMAKSFLKSEPPYSRLKRITRPAIEEGRGFYVSKVPFGWALYVADPVVAKQILLKSDIFPKSTRRIETLGKNSPLFKYFGFDNVVLSNGEQWKDQRRIMNPVFHRAMPIKTMARVIPSLFSVIEDADGRIPISLRMKDFTLDVLGLAIFDFDFKALKGDPENWTSTYTQLTEALFDPIINVFASMENLLIYVVPKRRKAAKAVVRLNEKFDQLVSTKRQELQNGRFSNKPDNEKDLLTLMLEAELQGEALETSEQLRHNIAVFFLAGHETTAHSLSFCLYQIAKDKHIQQKLREEIISVLGDNHMDVEPTLEQLKQMKYLNMVIKENLRLNGPLDLLPSRKAAKDTYLKDIFVSADTEVVIDACAIHRDPKNWKNPDDFMPERFGEGGGQEDHEGLTWVPFGNGSRQCLGMNFSLMEQRLVLTMLLRKYEIDVPKDSIHYDRIQYEKPLSIAPNTLELTFTKRY

 

>CYP509C9 P450-36 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

60% to P450-33 in CYP56 clan

RO3G_11017.1 revised

MENQIESYRLAIEKLLPAIQKRSKLSCITFAIALIAAQQLYSFFRVPKNLRQFPRVPYFSMVKSFLKAESPTDRYKRIIHPVASKGNGFYVSKVPFLWAVYVTNPVVAKQVLLKSDIFPKNHAVFHQIGKDSPFTQFLGLDNVALSNGDVWKKQRKVMNPAFHRSLPIKTMASVVNTLFSVIDNSDGPVLITSAMQNFTLDVLGLAIFGFEFKALQGDPDNWTKTYKTVIESLFDPVMNVFATFDTLLTWVYPKRRECSVAISKMNLKFDELAKQKRAEVKSGAYANVPDHDKDLLTLMLEAMEKGEALTSQDELRHNIAVFFLAGHGTTAHTLSFCFYHLAKNKHIQRKLREEIISVLGDEPVDIVPSLEQMKHMKYMNLVIKENLRMNTPADTLFTRDTVEDINLAGHIIPKDTAISIDINAIHHDPKYWHNPEHFIPERFAEGGEQESHEGLTWLPFSNGSRQCIGMNFSLAEQRLVLAMLVRKYEIDIPKDSIHYERILFDRPGNIAPLSLGLTFTKRY

 

>CYP509C10 P450-42 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

60% to P450-36 in the CYP56 clan

RO3G_12495.1 revised

MELVQFVDKSYREILEKLLPVLQKKSKWSYISAAALLITIQQIYSYLHVPKKFRHIPSVSSLAMAMSFLRHESQADRFKRIIQPVMNKGNGVYVSKIPISWTVYVANPVIAKHILLKSDMYPKSHNGFKIIGPNSPFLQFIGLNNLALSNGHTWKKQRKLMNPVFHRSMPVKMMSSVLLTLFSAIEKADGTIPIAEAMKHFTLDVLGYTIFDFDFKALKGDPDDWTKTYHLVNEALADPVLNVFPSMGALLLVLLPVKRRRMAAIDKLNRKLDEMAQKKRREIQKGSYSNKLDSEKDLLTLMLEAENNGEGLLSDTELRHNLATFFLAGHETTANSLSFAFYYLAQNKHVQQKLREEVISIFGDEPTDIAPTLEQLKQLSYLDLVIKETLRIAGPIDRIVPRVVSEDIVVGGAFIPKGTSANIDLYAIHHNTNIWKNPDQFIPERFAEGGEQESHEGLTWVPFGNGARQCIGMNFSLAEQRIVLALMVRKYIIDIPKDSIHYDHVVFDSTETKAPESLKLKFTKRH

 

>CYP509C11 P450-20a Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

62% to P450-12, in the CYP56 clan 35% to 509B1

RO3G_06942.1 revised

MEIVESVFKLYHIALENLLPILHKKHKKSYIGAAISLVFLQRVYSYFRAPKHLRHMPIIPYFSMVKSLLNHELAYSRFKRLILPAIGENNGIYVSKVPLDWTVYIANPMIAKYVLFHTDTYPKSHDMLTKLGSDSPLVQLIGYDNILISNGHLWKNQRKLVNPAFHRSMPIKTVSSVIFDLFSAIEKENGTISIAPKMKDFTLDALGLTLFGFDFKALKGDPDEWTKVFRQGIQVAMDPFLNVLSPLYSVLSFIFPEKRRQMKAVIKLNGKMDQMIKQKHTEVLSGAYSDIPENEKDLVVLMLEAERRGEGITNDLQLRNNMALLLLAGHDTTATALSFCFYNIAKNKHVQQKLREEIIGVLGDEPVDIVPTLEQLKEMPYLNLVIQENLRLNEPLNYLIPRVAKKDMIVDDILIPKGTTINFDIYGIHHNPKYWRNPNQFIPERFAKGGEHETHEGLTWLPFGDGSRRCIGMNFSMFQQRLVLAMIIRKYEIDISKDANDHRHIAGFKTKSRGSPKLTFTKRY

 

>CYP509C12 P450-12 Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

52% to P450-7_var1 in the CYP56 clan, 36% to 509B1

RO3G_05077.1

MMEIAEFALESYRSTLEKILPVLQKKSKKSYIGAAITLIVLHRIYSYFKVPKRFQHLPKLSYFPSAKSIFNNEPIYDRYKRLVFPVIKENNGIYVSKISFDWTVYIANPVAAKHVLLKADLYPKSHDFLKMLGSNSPVVQFLGYDSVGLTNGHVWKNQRKLMNPAFHRSMPINTMSTVIPDLFFVIEKEN

GTIAVPSVMRDFTLDVLGLTVF

GFDFKALKGDPDEWTKTFTLANEGLFDPILNIFGPFSFILTAIFPKRREQLKAVAKLNGKLEQLIHQKRMEIENGAYSNTPENEKDLVALMLEAEKRGEGLTNDLELRHNIAGFFLAGHDTTANALSFCFYNLAKNKHVQNKLRQEIISVLGDDPKDVVPTLDQLKEMPYLNLVLKENLRLNGPADNILPRVAAKDMVVDGTFIPKGATVNIDIYGIHHNPKFWNNPDDFIPERLDENGEQDSHDGLTWLPFGNGARQCLGMNFSLTEQRLLLVMMIRKYEIDVPKDSIHYERVIFGSETTPPNSLELTFKKRY

 

>CYP509C13P P450-22 pseudogene Rhizopus oryzae (Zygomycete fungus, Mucoromycotina)

53% to CYP509C10

45% to CYP509C9 in the CYP56 clan

AACW02000137.1 (-) strand

52294  SKIPLNWTIFITNPMIAKYILLK  52226

KDRYSKSHDVLNMLSPNS 52122

FVRFMDLQNVAFAIEQNWKKQQKILN ()

NTVFNRSMPAKTISNVLSTLYSDIEKEDGTDHIIEAMKNVALDVLG ()

HSYHNFEFKALQENPDE*TKTYEFVNNVLFNPVLNFFNTLSRLLSIVSPTRRKTMAAIDK

FNGMARQNRV*LQNG &

SMFN*PDTEKHLLTLISEAEESGEGLLSNTELRIRVPSPPSFFLRK*YKLVNN*QN

LATFFFA (1)

GHETT (0)

QTLIEEIISTLNDKPVEVLSTFEQLK*MEYLDLVIMEI (0)

TLCLAGPFDTIVPIFATEDIVLSSNLIPKGT &

50948 RNNIDVYAHHNPDYWKDHDQFILERFLKGEECGSYGGLTWLPFGNGVRQCIGMNFSLPEWRIALTLMSK 50742

 

>CYP509C14 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

40% to CYP509C4 Rhizopus oryzae

fgeneshPB_pg.49__3

first amino acid is I = ATA instead of M = ATG

conserved location for M in CYP509C15 and CYP509C16

IDTITNIFLSFETFVKNVVDYLPKGSYVVAA

MCLFFCKKVYDFTAVPYELKKFPNIPFFYFVKSILSVESVENRTKRLVLPLLHKNNGFYVSRFPFYWTVFTTDPDAVQYL

LMKGEIFPKDTRFTSAVSKDSLIIRLFGSSNVAFISGEPLKHQCRTMNPAFRRTAPVNIFGRLIPDMFRLIDKSDGDILI

VNLLQRMTFDALGKALFGFDFKTMKEDNSAWMTAYSDAMSGVTAPVLNIIPSLEYILRYFYPNYTKAKNGVDKLNRLILE

LVNKKKQELEESMPQSCSNNNNGDTDGDAYDQEKDLLTLILEAEMKDNKSSGSDDLRANMATFIMAGHETTASSVSFCIY

HMVINKDVRDKARREALDILGYDDFISPPTFDECKRVNYINMVVKEALRLCTPGGLLFERIATEDVFLSGVFIPKGTRIS

VDIEALHKNPAIWKNPSVFDPERFSKGGEHEQHRGITWAPFSDGNRKCLGINFSMTEQQVILLMLLKHYEWDLSENSIHN

NGMVYDNVFSFAPKTLSIKFHKRH*

 

>CYP509C15 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

67% to CYP509C14 Phycomyces blakesleeanus

e_gw1.3.167.1

MDIVNSISLSFGPSAKRLVDYLPKGSYVAAAVV

LFLCKKVYDFTTVPDKLSHFPSVSYFSFLRSTLAVESIEDRTRRFILPLLHKNNGFYV (0)

SKFPFYWTVFVTEPLAVQYLLMKG (1)

EIFPKDSRF

MDALSKDSLAVRLFGNSNVVFSTGDLWKHQRKAMNPVFHRTVPVDLFGRMIPDIFKLIEKTDGNVLVDSFLQKITFDALG

RALFGFDFKTMGDDDSEWIAAYNDAMSGITAPILQVATSYERILRHFYPHYDKASKGIDKLNKLILDMVDEKKKKIEESK

GQPDAGHEKDFLTLILEAEMKENAISDPDDLRVSKTKCLSGHLSTASSIAFCIYHMAINKASLKDIQNKARREALDILGD

DDSMVPPTVAECKRVNYINMLVKEVLRLCSPFGAILERMTAEDVTLSGAFIPKGTLVSVDIEALHKNPAIWKNPTVFDPE

RFRKGGEYDQHKGITWAPFSDGNRKCLGVNFSMAEQQVVLLMLL

KHYEWDLPENSIHKNGIVYDSIFSFTPKSLSIKFHKRH*

 

>CYP509C16 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

67% to CYP509C14   Phycomyces blakesleeanus

fgeneshPB_pg.3__395 revised

MDTINHISPSFESYITVFAKIVPKGFYVVAAVALFLCKKVYDFTAAPYKLRHFPKVSFFAFSKSIFSAESVEHRTKRLIS

PLLHKYKGFYIAKFPLYWTVFVTEPHAVQYVLMKGEIFPKKTRFMNTLNKDSLMIRLFGSSNIAFASGEIWKHQRKIMNP

AFHRTAPIELFGRMIPDMFRLIDKSNGNIMLADLLHRITFDAMGKALF (1)

GFDFKTAREENSEWTSAYNDAMSGI

SAPILNITPSLEHITRYLYPDYAKAKKGIDKLTELTLEMVNERKEKIQEAIGQPDDGREKDFLTLIIEAEMKEDKASGSG

GLRENLKAFLVAGHASTASSISFCIYHLAMNKDVQNKARKEALDILGDDANISTPTVNECKHITYINMIIKETLRLNAPF

GTLFERIATEDVTLSGVFIPKGTIISVDIETIHKNPAIWKSPTVFDPERFSKGGEHDQHEGITWAPFSDGNRKCLGINFS

MAEQQVILLMLLKHYEWDLSENSIHKNGIVYDGISLFTPRSLYIKFNKRH*

 

>CYP509C17 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

76% to CYP509C14   Phycomyces blakesleeanus

e_gw1.3.168.1

MDIINNISLSFKPFVKSLVDYLPKGSYVAVVVALFLCKKVYDYTTVPYELKHFPSI

PFFSFIKSVLSVESQEDRTKRLVFPLLHKKNGFYV (0)

SRFPLYWTVYTANPEAVQYILMKGEIFPKDTNFMNVLSRDSLTIRFFGSSNVAFISGEPWKHQRKTMNPAFRRTAPVDLFGRIVPD

MFRLIDKTDGNIFIFDLLQRVTFDALGKALFGFDFRATVEEDSAWRAAYIDTMAGVTAPILNIIPSLEYILRYFYPDYNK

ASKGVDKLNALILDMVNKKKKKLEESKDQPDGGQEKDLLTLILEAEMKENKSSESDDLRANMATFIMAGHETTASSVSHC

IYYMAMNKNVQNKARREALDILGDDSSTAPPTFDDCKRINYINMIVKEALRLSVTSGALMDRVATEDVTLAGEFIPKGTY

ISVDIEALHKNPTIWKNPTVFDPERFSKGGEHEQHRGITWAPFSDGNRKCLGVNFSMAEQQVLLLMLLKHYEWDLSENSI

HKNGIVYNSIFSFAPKTLSIKFHKRH*

 

>CYP509D1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

49% to CYP509B1 Rhizopus oryzae over the last 405 aa region

fgeneshPB_pg.25__98

MLAKKVNGYAGFTLDAIQTIYFKYIFPTTGHQNRSSIIAYLTALILLYMAYSRAIKLVRPPKALSHIPHVPYFSYMSSLI

KKENTISRNKRFANKLFEDPESNGLYLRPNVKGWEVVVTRPEDAKKLLFKSDIFPKADFTSGIDGTILSKFSRGPNLLFT

TGAHWKAQRMVANPAFHRSAPVKLFGELTQKLFRVMDNRANKTVDITRLMEAWALDAIGLAGFDFDFNAIENPNSSWVRI

YERVDKALVHPFYSFFPKADKYLLWVLPNRKQAHTDLDIFLKMIDNVIIAKKKALHENKSNKHLENSEKDLLTLMLESEA

KGSTLSAKELRSNMCVFFAAGHETTANSLAYAIYFMAVNPDVQCKAREEAFKVLGDAQEDIMPTIEQTKSMDYINAVIKE

TLRSHSPALGTFARKATKDTELGGVLIPKDTMISMDIFNLHHNPNVWNNPDEFDPSRFLPGGEAEKQINNGFSWIPFGTG

ARQCIGMNFSLIEQRVMLSMMLRKFAWSLPEDSIHKDYLHTTNLVISSAKDLNINFEKLY

 

>CYP509E1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

43% to CYP509D1 Phycomyces blakesleeanus

e_gw1.11.60.1

MANSSFENLQSLFVDFVQPQLVQRKKQAGVIFSAVVLVLCYNTINKIIYPPKSLRHIPHV

NYIAYTRSQLRKQPASEQARELLLPLLAENNGFYA (0)

IPFLGKWSVQVANPIAIKTILLKF (1)

DMFPKANSLSKSQGTLAHRFIGGPNVFLLEGKKWRKQRMISNPAFSRSMPVDMFGRITINLFKYLDNIDPTIDVLDTMKK

WTLDTLGSAVFDFDFESLTKPNNEWTSIYYEINASLFVPIFNILPVLEKSFLWMFPKRKRVHDKMTKLKDMMRQVIIQKQ

ARLKENKPNPNLKDTEKDLLTLLLESENEGHEPMSEDELMSNLCAFFFAGHDTTANALSSALYHLAVQQDVQKKAREEVI

NVLGDEPEDVIPSIEDTRQLDYLNLIIKENMRINPPVGGPLDRLVTEDIVLDGVLLPKGTSVKVAVYSLHRNPLLWDSPE

EFRPERFLPGGEADKIEGMGYIPFSDGGRQCIGMNFSLVEQRVLLAMMLRKYTWKLSENTINKDELQVYAFNIMAPFDLK

ITLEKRY*

 

>CYP509F1 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

51% to CYP509D1 Phycomyces blakesleeanus

e_gw1.20.54.1

MSSYQLSGYTGLTLNQLQVLYSKYLVPTLKDKAKSTTIASAVALLLGYLAFKKIQEP PHEIRHLPHISYFTMLRSILQGEPTSSLSKRLVIPIISCPENNGLYA (0)

RINGLGWTVHVASPEANKKMLMRTDIFPKSDPLPGTDGTLASRFLRGPNVLLLNGASWKTQRKLMNPAFHQSIPVNMFGG

LVQRLFSVMENETGPINVTNLMMRWTLDVIGQAGFDFDFHATDEPENEWVQVYNSISKGMGKPLWFFFPVLDRKFLWLFP

ERRKSHKSLDRFLEMLDGVIVKKRKALKENKRNPSMKDNEKDLLTLMLEGSQTGGAALTDRELQSNLCIFFFAGHDTTAN

ALSFIIYFFAKYPKIQQRAREEAHKILGDAPQDVIPTVEETKDMTYINMVIKETLRIKGPVTAIPARRAAEDTELEGVFI

PKGTLLTSDIYNMHHNPKIWNDPETFDPERFKAGGEADMLASSGMPWMPFGGGSRLCIGMNFSLAEQRVMISMLLRKYTW

RLPEDSIHKDELRTNNIGIMSAKILDIIFEKIY*

 

>CYP509F2 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

53% to CYP509F1, 53% to CYP509F3

e_gw1.20.55.1

MSISSLTAHTNYALDIIQNFCTNQDVQNLVKGNLPYISLAIVTTLAYITYEKVCRPPPS

LRHIPSIPYFKFMRSMLKGDTAAIHSKQVAIPLLSGKNHNGFFV (0)

RPNIRGWEVFVANPEAVKQIFNNIDIFPKSEPLSDSDGTLFGGFIAGPNLAFLSGAAWKTQRKLINPAFRQSMPVNFFALSTQRL

IQVVDKSNDQVNVTELMGRLTLDVIGKTGFGFDFHAIDNPGNEWVRIYNNITRGLYRPLWFLVPILERKFLWMFPERQKV

HQDLNKFLSMLDIIIDKKHKDMKEMKDLQLENNEKDILTLMIEGAAEGNGILSYKELQSNICVFFLAGHETTSNVLSFVL

YYLAVNPELQHKARDEALRILGDDPKEIFPTVEQTKHMEYITMVIKETLRLRPPAPSVLTRTCAADTVVAGVFIPKGAFI

APDIYDLHHNPDIWENPETFNPERFRSGGEADILTNNGMPWAPFGGGTRLCIGMNFSLTQQRVVLSMLLRKYTWTLPEDS

IYKDDLHTNAIGTLTAKKLEISFRKRY*

 

>CYP509F3 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

54% to CYP509F1 Phycomyces blakesleeanus

fgeneshPB_pg.20__100

MISNQVSECAKFTLGYIKDIYIKYFLPTFLTNKRGPAIASAAAIVLLYMAYDKLFSPPRAIRHIPYIPYFSFLKSLLQST

SIAEHNEAFVFPIINQKNTNGIFSHPGIFGWEVAVANPAAIKKIMLKIDAFPKADVIRTTEHTIISRFLRGPNLVFSNGS

SWKTQRKIANPAFHRSLPVKLFGDMAKKMFQVMDKQDSLVDITDLMERWTLDVIGEAGFGFRFNAVEDPTNNWVQLYKNI

SNGVNDPFWFFFTNLETNFLWLFPKRQRTHRELDTFLEMLDDVIVKKRADIASNNKNPHLQENEKDLLTLLIEASKEGSG

SLSDQELMSNLCAFFVAGHDTTANALSFTIYYLAKNPDIQQKAREEAISILGEDQDTIPTIEQTKEIDYINMVIKETLRM

NSPAVSAITRISTEDTELDGVFIPKGALVVPDIYALHHNPTIWHNPEEFNPERFVPGGEAERLSGAGMCWIPFGSGARQC

IGMNFSLAEQRVMLSLMLRKYTWSLPEDSIHKNMLQATKIGVMVAHDLNIVFKRRY*

 

>CYP509F4P Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

54% to CYP509F3 Phycomyces blakesleeanus

fgeneshPB_pg.20__103

SNV*IFFVATHDTTENAQSLAIYYLARNLEIQHRERKNVINISGEDKQDIIPSVDQTKGIIYIKMPIKATLGI

KEPILATLTQISLGVTVTVNLEIVL

 

>CYP510A1 AB045779 Lentinula edodes

MYTTISPMLVVSLATLAFISLFNIYRYRYRPSTVNSPYPPGPKI

ATMPTRDAWVEYREWGNNYGGLVYLPERNTLIANKSEIAIELLEKRARIYSDRAMTPI

IKLCGGEDILSLERYSEKWRIDRRVFQQTFRQAASSRFYPAQYNKIHEFLRQLLMTPD

KFMQHTMKLSQALVYKGLYGLDVDSEDPLAKKAVEVMDTLGQALLPGSFPAYERFPFL

RFMPSWFPGCRFKRIAIQCSQGVKEVDTIPFDIAMAHLESGTGTSPIAELAIRKPQDI

KAIKAMGTISYLASADTTMSSISSFLLTMCQNPDIQAKGRDEIERVIGTDRLPTFEDR

QSLPYVEAIYREVMRLHPALPLGLPHVSIEDDFYRGYHIPKGCVVIPNIWAMNRDPDV

YTEPDRFLPERYSESPTGPFESINNIYAFGFGRRVCAGRHMAENTIWLTIASVLATFT

LGKAKDGKGNEIDIPGEYTDQFFRHPKPYRSSIFPRSTLARELVLATNAE

 

>CYP510A2P    Lentinula edodes (a basidyomycete fungi)

             GenEMBL AB015310

 PLLRAMNRNPNVYQDPDRFLPECHLKSAAGPFESIDNIYAYGFGR

VCAGRYMADNTIWLTVASVLAAFTMGKAKDGNG

RYPKPFQSSIIPRSQAAEILVLAT

 

>CYP510A3     Lentinula edodes (a basidiomycete fungi)

             GenEMBL AB049963

MMYTAFFGLLAVATLALIGLVGIYRGRRQPYTAAGSCPPGPKVA

IMPTHDAWVEYKKWGNDYGDLIYLSERNTLITNKSEVAMDLLEKRARIYSDRAMTPIM

KLCGGEDILSLERYSEKWRKDRRVFQQTFRRAANSRFYSAQYNKIHTFLRQLLLTPDD

FMEHMLRLSQALVYKALYGLDVDPEDPLAKKAVEVIDTLGQALLPGAFPAYERFPFLR

FMPSWFPGCRFKRTAIQCGRSVKEVDTIPFDIAMAHLKSGTGVSPIAELAMRKPQDIK

AIKAMGTVSYLASADTTMSSISSFLLTMCQNPDIQAKGRDEIERVIGTDRLPTFEDRQ

SLPYVEAIYREVMRLHPALPLGLPHVSIEDDFYRGYHIPKGCVVIPNIWAMNRDPDVY

TEPDRFLPERYSESPTGPFESINNIYAFGFGRRVCAGRHMAENTIWLTIASVLATFTL

GKAKDGKGNEIDIPGEYTDQFFRHPKPYRSSIIPRSALARELVLATDAE

 

>CYP511A1 Botrytis cinerea AY277723

MGLLSDVINNFLPETPLAWAALILASFTLYSVQLVVRRLYFHPLSKIPGPFLARSTYWYEFYQDIILGGIYVKNYAALHE

KYGPVLRASPDRVHVSDPDFFHEVYSSGSKYMKDPAFFQAAGGIPEALPAIVDVEYHRRRRKLINDLFSAKSMEALSHLV

LKVVQNALSKAHEHHEANKVLDIQRLYTGITIDTIMQVLCDRTLNFIDAKEEEEPPFLATLRTFSENFFLLKHFPILIWM

ALNIPKSIAQKLIPGEFEFRASINQWIRDRASEHELGVEKAEDGRKTVIDLLLRPEDGGRPLTHQAVEDETYSFAFAGTH

TTSHTMSMGTYYLLSHPAKLQKLRDELKPIPKNDQGLYEYKTVRSLPYLNACIKESLRMSSPVPGILPRLVPAEGMTWRG

HYLPPGTSVSSSIYSVHTDPNIFPNPEQFIPERWLANENLDHYLVVFGKGSRACIGLNVAWMETYLTFSNFFTSLNMTLF

ETNEQSTDWTDCGNAMIKKHVRVKVDSLAS

 

>CYP511A2P Fusarium oxysporum

44% to CYP511A1 Botrytis cinerea, N-term, cannot find whole seq

FOXG_05899

78% to CYP511A2P Fusarium verticillioides, probable ortholog of the pseudogene

WATTSTS*

NILYYTAVAFGVFGFYWLCLVFYRLFLHPLRNVPGPKVAAATSWYEFYQDVILDGHYIKDYPRLHERY (1)

GPIVRMSPNRVHINDPNFYHK (2)

VYSTGNKFFKEPAMFRFARELDALPFIMDPAARKVRR

GIVNPMFSPRAIKDFSPLALQIIKGALKKIGDSYETGKPVSLKRLESNFA (0)

 

>CYP511A2P Fusarium verticillioides

78% to CYP511A2P Fusarium oxysporum

FVEG_03770, probable ortholog to CYP511A2P Fusarium oxysporum

WSPTSTSW

NIISCTTLAFGAFAFYWLCLVFYRLFLHPLRNVPGPKIAAATSWYEFYQDVILDGHYIKD

YPKLHEIY (1)

GPIVQMSPNRVHINDPKLLPQ (0)

GVFYGTKFLREPAMFRFAGELDALPFIMDPAAHRVRR

(large deletion here about 140aa)

GRQTVFDAILDAEPDHSTKGLVDVAFSLVIGGTETTVTTITYGVWCILKNPNVEKKMLEELRTVETNSDGLVEYPNLMNLPYLTAVIHKT

LRISSPAPGILTRLVSSGGTKYGSHFLPADTSVSTAQRLIHYDPVLFPEPETFMPERWLDNSDKASKKNLIPFGKGPRMCVGLNLSYMQAYVFLGNLIRR

FDLSLQDKDQGILRWKDCIALHIEDDVLIKVNGLR*

 

>CYP512A1 AB057426 Coriolus versicolor

MEDPTVLYACLAIAVATFVVRWYRDPLRSIPTVGGSDLPILSYI

GALRWTRRGREILQEGYDGYRGSTFKIAMLDRWIVIANGPKLADEVRRRPDEELNFMD

GLGAFVQTKYTLGEAIHNDPYHVDIIREKLTRGLPAVLPDVIEELTLAVRQYIPTEGD

EWVSVNCSKAARDIVARASNRVFVGLPACRNQGYLDLAIDFTLSVVKDRAIINMFPEL

LKPIVGRVVGNATRNVRRAVPFVAPLVEERRRLMEEYGEDWSEKPNDMLQWIMDEAAS

RDSSVKAIAERLLMVNFAAIHTSSNTITHALYHLAEMPETLQPLREEIEPLVKEEGWT

KAAMGKMWWLDSFLRESQRYNGINIVSLTRMADKDITLSDGTFLPKGTLVAVPAYSTH

RDDAVYADALVFDPFRFSRMRAREGEGTKHQFVNTSVEYVPFGHGKHACPGRFFAANE

LKAMLAYIVLNYDVKLPGDGKRPLNMYWGPTVLPAPAGQVLFRKRQVSL

 

>CYP512B1 pc.30.92.1(genewise2nd.30.46.1) Phanerochaete chrysosporium

MSLHQVYDAVVAHGDMGTLLVYMAYSVPLVMFLYSLFSPASLRHIPTEGGPSFPLLSYKAARAYLRDATGILQRGYDKHKGKPFKVAMPDRWVVVLTGKKLVDELQRLPDDAVSFIKGASDLSGTEHMFGRQVIDDPFHVPIIRTHLTKNLAPMFSDVFDEVSIAFQELIPACDGEWVPVHAIKVARSVVARTSNRIFAGLPICRHPEYLNLVINFTVDVAKGRYALLLFPPALKGIAAKILTNIDGRIKEGLKYLGPLIEQRMALAEKFGNDSSEKPDDMLQWIIDEVRARNQSVFEVVRTVLLVNFAAIHTSSNSFTHALYHLAANPEFIAPLREEIETIVSEEGWSKAAIGKMWKLDSFMRESQRYNGINSVSVKRKALKPLTLSDGTFIPKGTVLVTPTVATHFDDDNYKNPTVFDPFRYYREKEQDMSAVKHQFVTTSPDYVSFGHGKHACPGRFFAANELKAMMAYVVVNYDVKFEKEGVRPENIYAAMGISPDPNARVLFRKRESIVSV

 

>CYP512B2 pc.30.93.1 Phanerochaete chrysosporium

MTPSHSFPDLVASCISAWTLCFALGFSIAAASFYSLFGPFNLHHIPTVGGSSIPLISHRGARKYMRDAKGVLQDGYKHKGKAFKVALTDRWLVVITGKRLVDELQKMPEDVASFVGAVADFQGLRYIFGQKVLDDPFHVNIIRSHLTKHLSSVFGDICDEIYVAFSELIPQQDEEWVPVHAIQVVRTIVARASNRVFVGLPVCRNAGYLSLAVNFIVDVAKARDFIALFPPVLKPLAAKMTSDIGTRVQEGMQYLEPLINERLRLMEKFGKDWTDKPNDTLQWMMDGIMERDGTIEQLVRIVLLENFSSIHSSSNTFTHALYHLAANPEYITPLREEVETAISEEGWTKAAMSRLRKVDSFLRESLRLNGINPVSMQRKALISFTFSDGTYIPKGTILVTPALATHHDEDNYEDATTFKPFRFVGENPEDDVPLVTTSADFVPFGHGRQACPGRFFAAHQMKAMMAYLVLNYDVKFENEGVRPQNVHGVLSVQPDPKARVLCRRRKSSYT

 

>CYP512B3 pc.30.113.1 Phanerochaete chrysosporium

MASNHLFSGVLPLDRAASTLGYLVCGALLALLLQNILTTISLRHIPTVGTSTLPLLSYKGAYDFTRDIKGVFQQGYAKYKGRAFKIAFTDRWFVVLTGRKLLEELHRLPDSTTSFNHASGSITGSTYIYGRGWLSDPWHIPIIRDRLTKHLAASFGDMYDELETAFRELMPSCEEEWVPVHFITMARTVVARTSNRVFVGLPACRNLGYHTLLVNFALDVSKARNRLAWLPPALKRVAARALTRIDSRIEEGMQYLGPTIRARIVEMERYKGDWPDKPNDILQWIMEELIARKMPMEEAVRIILRINSSAVQTTANSLTHAIYHLAANPDLIAPLREEVDAVITDEGWTKLAMSKLSRLDSFMRESLRLNIVNPLSVRRMALKSFTFSDGTFIPKGTLMVTPAHATHLDEANYEHASVFDPWRFVHQKEEDLSPTKHQFITTSPEFVAWGHGKHACPGRFFASNELKAMMAYIILNYDVKFARAGVRPDNVYSGLTVAPNQEANVLFRRRQTQ

 

>CYP512B4 pc.30.114.1 Phanerochaete chrysosporium

MAFSDVVATVGAGPWVAYMMCAVLLALLLYSLFSPASLRHIPTEGGSSLPLVSYLGAYNVLRNLQSVLQRGYDKHKGKAFKIALPDRWVVVLTGKTLVDELQRMPEESASFIDATTELTGFGYIWGPRMRKDPCHVPIIRNQLTRQLSSAFGDIYEEIELSFQGLMPACEKDWTPVHVIEVARDVVARASNRVFVGLRVCRNPDYLDMLVDCAVSVASARNTLMLFPFVLKTFAAKNVVNMDRRIRRGMQHLGPIIEERMSLLRSLGNDWPDKPDDMLQWIIDEVAARQMPKEDVVRNIMFLNFAAIHTSSNSFTHAIYHLAANPDYLGPLREEVEAVTAKEGWSKTAMGRMWRIDSFLRESQRVNSINPLTVIRRTRTSLTLSDGTFIPEGTVVAAPAYPTHFDDENYVGGDTFDPWRYVREKEQDLSPSKHQYVTLSPEYVPFGLGKRACPGRFFAANELKAMLAYLVVNYDVKFEKEGVRPENMHVGLTISPDPAAKVLFRKRRS

 

>CYP512B5 pc.30.118.1 Phanerochaete chrysosporium

MARSDILDALSLGRTELSTTYLVFGLFLALFLYSLFSPASLRHIPTVGSSSLPLLSYKGAYDFLRDGRSLLQRGYNQYKGKVFKVAFTDRWLAVVTGRKLVEEVQRLSDDVISFPDASGEVTGFKYIFTKCALSRDPFHVNMIQRQLTKHMSVAFDDLHDEFETAFKELLPHNETAWVPVHAIEVARKVVARASNRIFVGLPVCRDKAFLDLMVNFTLDVARARDLLALFPPALKPFVAKLVVKLDSRIEEGMQVLRPIIQERMEIIEKFGKDSPEKPDDMLQWIIDALVERNEPMEQVVLITLFVNIAAINTSSNSFTHALYHLAARPEWIAPLREEAEAVIGNEGWTKNAMGKLVKIDSFMRESQRYNSIVPLTCMRKALQPFTLSDGTHIPRGTILVTPAIATHFDDEHYADAASFDPSRYVPVADAKQGGAPKQYVTTTAEYVPFGHGKYACPGRFFAGTELKAMMAYLVLNYDVKFAQEGVRPPNAATTLSTRPHQEARVLFRKRNSSVQ

 

>CYP512C1 pc.30.76.1 Phanerochaete chrysosporium

MSSMNAPALPATHIVAGAILVWLLVRTFGTQNLRHIPTEGGPSLPIISFLGLHAFLTRSREILEDGYQTHKGRAFKVALIDRWLVVLSGKKLVEELQKMPDDTVESATTEMFNMQHVFASNWHKDPVHSSLLRSLTRNLGVVFSDMFDELDTAFRECVPANAERWLPVQAHTTMASIVTRAANRIFVGLPVCRDAGYIHMMIHVAEDVSDAVRTLSMLPTFMKPFVARRATVIDQRIQQCLDYLRPAIADRMSMLERFGKDWEEKPNDVLQWIIDEVTARNQGEDEVARIVLFINFGAIETTSFAVTHALYDIVSRPGLADVLREEVEAAVATEGWTKAATNKMRKLDSVLRESQRLNGPTTASMFRRVLQPVTLSDGTYLPAGTTVVTPTLATHFDDTNYADAQTFDPLRFYKPDGVQAQLVTTSADFVTFGHGKHACPGRFFAANELKAMMAYILMHYDIRPEREGVRPENVYRGLNVLPDANARVFFRRRQTD

 

>CYP512C2 pc.30.77.1 Phanerochaete chrysosporium

MVLTTDFGGISTTHVVIGAFVTWLLLRYFSAKNLRHIPTVGGPSVPILSIVALYNFLANGKKVVLDGYQKYKGKAFKVALLDRWLVVLCNPKLVEELQKLPEALVGYSLLEAXGTIFETKHIFGADLLTDPVHLVLLRTLTRNLGQVFGDMYQEVETSFQELVPANEKEWLPVHASPIMRTIVTRAANRVFVGVPVCRDEGYLHLMVHFAEDVNKAFGLYTVVPSFAQGFVARKAKAVMDDCIERCLGYTRPTIKDRTTMMDSFGDNWADKPNDMIQWTIEETKARGQGEYDMARMLMFINSGAVETTSQAVIHALYDISVRPELADELREEVERAIAEDGWTKDATNKMRKLDSFLRESQRINGPMIVSMFRLVREPVTLSDGTFLPAGTTIASPTLGAHFDDSIYPNASTFDPLRFYKAEAAGQPQFVTTSPEYLTFGHGKHACPGRFFAVNELKAILAYMLMHYDIKPEQDGVRPENKSMGLGVLPNPDAKVMFRKRHAG

 

>CYP512C3P gx.30.36.1  60% TO 512C1 PSEUDOGENE Phanerochaete chrysosporium

NDLLQWIMDEAVARDKS (FRAMESHIFT)

QEEIARMVLFLN (FRAMESHIFT)

FGAIQTTSCV (1)

LSMFPNILTPVTLSDGTFLPAGTTVVTPVLATHYNEDNYTNAALFDPFSCKDNRSGGQQFVRTSADCVTLGHGRML (1)

CPGRFFAAAELKTLVAYVLVNYDLRPETEGVRPVNIYKGLT

V*PSETAKVLFKKRQTDE*

 

>CYP512D1 pc.27.9.1 Phanerochaete chrysosporium

MQSSVGEVFASAPTLAKLLAGAAFVLFLNSLWNIWKLRHIPTVGGPAIPILCYIGTFRYLQDPQKILQEGYEKYKAKPGMFKIAAPDRWLVVVGHPNLIDELQKHSDEQVSFMDAATEFVGTRYALPGTIADDPWHIPPLKQHLTHAIGSFFGDMLEELRVSIEERIPSNEKDDEWVAIPALDTFFWVFTRVIDRIIVGLPIRDTEFIKLMVEFTMSIGIARFFIGLVPPMLKPAMAKLAARGVHKATAEAEKMLAPVIADRVRHLDEFGEKWADKPNDLLQINIEEARAQGRPLDEIVIRTIVSIFVGVSTSAASFVHVLYHLAADPELQAALRTEVEGAIARDGWTKAALVGMHRVDSVLRESQRVNGINSVSVMRTALQDITLTSAGAPVCLPAGTLCVAPERALHADKEHYPDPDAFVPFRFAELRATADARGGAQHQFVSTSTRYVPFGHGKHACPGRFFAGNEMKAAVAHLVSNYDVRLPDGASTRPPNELFGLAIVPNRSAKVMSRRRQPVV

 

>CYP512E1 pc.154.15.1 Phanerochaete chrysosporium MSDYSSLLAYIFISLATLAYLKRLLWPDRQQLEHIPAIGPTAPILSYWGAFRWLSHGTEITQKGYAKYKGRPFKVANFNRWLVVVSGPKLIDDIRKAAEHELSFEEAAHENLEVRYTAGPCIAENSYHVPIVRGQLTRNLPFLFNDVRDEVAKAFGDHIPPTDDWTPVAAHPVIMQIVARATNRVFVGAPKCRDPDWLDLSIQFTADLILGAHIITQFPQFLKPLAARFFTRVPAAIRRGRRHLERTIEHRKACLEQYGADWPDKPNDLISWLLDEAKGEERTIHNLVTRVLTLEFAAIHTTSNSFVHALYQLAAHPEWAEPLRDEIEQVVKREGWSKSSLDKMHRLDSFLKESQRYYALGGVTMDRRAMKDFTFSDGTVIPEGTFVGVAVLATQHDPQYYDDPDTFNPWRFSDLREESDESGRHLLVSTGIEYFPFGHGRHACPGRFFAAIELKLMLAHIVMNYDVKAELDGVVPPILEFGQNLAPNMKAKVLFKNRQRS

 

>CYP512F1 pc.15.28.1 Phanerochaete chrysosporium

MISIDSISLISGLISFAFIAYYLRQDKLQHIPSPGPTGPISSWYAAYKYIRGDAPQIIEEGYRKYKGRIFRIADLNRWTVVVTSPSLVEELRKAPEDVLSFHEGIRYSLQLDYTFGREAVEHEYHIPVIRTQLTRHLTPLFADIHDEIVQSFTDLVPPSDTWTSVRVVPTVMQVVSRTSNRVFVGLPYCRDPAFCALAVRFATDVVKTGVALHLTPRALKPLGVRLVSPVSKRVEEGKGDHRQAGGRPAPRQPGGRPARAAQGACGNWPDKPEDMIQWLIEEANEDERTVEGLVLRILIVNFAAIHTSSMSFTHAVNMLAAHPECIAPLREEIEEVVREEGWTKAAVQRMRKLDSFMKECQRLHGLGAVTMSRVALQDYTFSDGTRIPRGTLVMAASRPIHHDAALYVPDADAFDPWRFARLRAADADASIKHQMVHTSAEYLAFGHGKHACPGRFFAVNELKLMMAHVLHTYDIRPQTSVPPGRWIRHSLLANPIATVDLKKRQT

 

>CYP512G1 pc.16.37.1 Phanerochaete chrysosporium
SVPTMGPTAPLLSYWGVLRYMTRPRDVLREGHVKFGGRPFRVASPLRWQYIVSSPELIDELRRAPDAELDPLAAADDILHFVKALGTKFASNTYHVPIVRTTLTKNIGTLLPSVLDEMRVAFARYIPADKEWHPVVAHDTNVRIVTQTSSRIFVGLPLCRDPELLKITMSYTPTVMKTGLLLKVLPRPLQSFVQRGSGSIDALIDRAHRLLLPTIEERRRMMDKYGAEWLDKPNDMLQWLMDSAEGEERTPRGLAARMLAVYFAATDTAALGFTVALYRLATHPEYVQPLREEVEAVIAQDGWTREAFRKMPKVDSFLKECMRLQGPSTLLLQRKAMQDFTFSDGTFVPKGSHVATSIVATHCDSAYYSDPLTFNPWRFVGAEDDAQDSKHRFATTSPEYLLFGYGRHACAGRFFAEIQLEMMMAYVVTTYDVRMEKPGVLPEPIEFGSMSLPSMTAKVCFRKRATE

 

>CYP512G2 pc.15.22.1 (genewise2nd.15.10.1) Phanerochaete chrysosporium
MFADSPTSLYALVLLGTVVYLLNWLKGSKYKSVPALGPTAPLLSYWGAIRFFLDAQGMLQEGQLKYGGSPFRIATRRYWQYIVSSPKLIDELRRAPDDELSFLDAVNEALELEYTMGAATANNLYHVPVIRNTLTRNLGNLSSEIYDEISNAFADCIPARDEWMAVPALQSIMQIVARTSSRIFVGLPLCRNREFLEISMTYTTDVVKTGLLLNMVPGPLKPIVNRLFSKVEQHIDRTHALLRPIIEERQRMMEQYGDDWPDKPNDMLQWLMDAAEGQEREPRALALRILIVGFAAIHTSSMSFTQALYYLAAHPEYMQPMRDEVEAVLAAEGGWSKGALQKMRKVDSFLKECQRYEGLGMLFLTRKAVKDFTFSDGTFIPKGSYVSTSRAATHGQSEYYRDPYVFDPWRFANLRDETGEGVKHQMVNTSIEYLPFGLGKHACPGRFFAANELKSMMAHLVVTYDVALDMPGEVPRSVHFGPINSPNRTAKVLFRKRRG

 

>CYP512H1 pc.21.108.1 Phanerochaete chrysosporium
LQDIPTVGGPNLPFFSYFGAIHFLVRANKIISDGYSKYKGGSFKIAQVNRWLVFVTDPALNEELRKAPEDQMSSPAALHQYVQGLYTMGFGLDGMLYYIEVLRDQLLRHVNPSPAMLYDEMQASFQDFIPQNTEWTPIPALSTSLKLFLRMSNRAFVGTPLCSNSEYLDLVTEFMNNVFKGAFFYNCLPAFVRPILARWLDLVNPCLDRAVRLLTPIYATRVAELEAAGKAEWAGASDDLLSCLVASHYSAARDVRELARILLVVNLAAVHTVSQSFTSVLFLLASRPAWQAELRAEAAAALAHGYTRDALARLRKLDSFVQESLRFNGLGALASTKLALTDFALSNGTVIPKGTLVSAPLRALHLDDEVYPDGASFQPWRFVRAGGEAAPRQSLASTSPTYLPFGHGKSACPGRFFAALELKMVTAYLVLNYDLKLEGDATEVPPVSWFITARVPNYKANVLVRRRQEKA

 

>CYP512J1 pc.37.85.1 Phanerochaete chrysosporium
NIPTLGTEMPVLSLWGALRYVMNSHNVIQEGYLKYKGRPFKIAQFDRWLVVLTTPHHIEELRKAPETGLSSRDAVDSLLKAKYTLGMDVDTVQRYLDLFREKLANKLGGLTSEVHEEMELTLNESLPKSEDWEEFCVLPSILKILFRTTNRAFVGAPLCRSEEYVALGEEYTTNVFKGAVIYNALPKALLPLLSKVLDFIGPTSRRCEQLFRPEMDKRAAYLEEHGADARGKYDDLLTWLIATHGAGDEINYSELTRIVLIANLAAVHSTAMVFTFAIFHAAADPGVADALRAEVASVVAEHGWTPTALTKMQRLDSFVREVQRMHTLGAALVMKIARTDYAFADGHVVPRGALVTAPATTVHRDDEHYPDAHTFRPWRFVGTPDESEADSARRKATSTSPTFLAWGHGKHSCPGRFFAVRELKMLLGSVLLRYDVRLKTPGVLPQDQWYLTFRVPDPTACVLLKRRTTA

 

>CYP513-525 # are Dicty sequences

no sequence

 

>CYP526A1 AF330109 Fusarium sporotrichioides TRI13 gene

MFLSLCLMVLALYLLYKWALPKPISSIPYNPPALQSLFGDIPAM

IQGTKANNQTHMDWIIQQMKNHESPIIQLFLSPLQRPTVILADFRETQDIMLRRKDFD

RSTNIRGLLEDVIPDHHIYEQTNSVFRTHRKLVQDVMLPSFIQKVAGPAFHSNIMRLV

RVWDLKAQIADGSPFLATQDIQGAVLDAVYSFAFGSYYKSSTTLPKIEKLEKWNGNAE

NSSRNAPQSDKPFDFPDVAFDDLINATIDLAKAPQGLQGSPIAKLQAKVTMNMPHFRR

VRKIRDDFLRGSLKSAVSKLPSEGGKSDSQAVTSAVEQMVLRETALAQTENRSPNYFS

TMMQGELFGLILGGFDTTSTTTLWGLKFLTDNASVQKRLRQALQSSFTKAKAENRSPT

FQELAVARIPYLEAVIEEILRCAGATPALQRLAKVDTQILGYHIPKGTDVLFLTHGPS

VWTPGFEIDESRRSQTCQAAGEKKDQCWESHDISKFKPERWLGQKLPSNNREKDTDAT

ETAEEFDGLAGPTLAFGLGTRGCFGRRLGYQQLKTSITILIWNFELLPCPQELSSYRT

IEGLTSMPEHSYISLAKVDLTTT

 

>CYP526A2     Gibberella zeae strain HKM136 (same species as Fusarium graminearum)

             GenEMBL AY057841.1

             cytochrome P450 (TRI13)

             82% to 526A1

             note: this sequence not found in Fusarium graminearum genome assembly

809   MFLSIGLVAVALYLLYRWALPKPISSIPYNPLALQSLFGDIPAMMKWTNASKQSHMEWII  988

989   QQMKNHESPIIQLFLKPLGNPVVILADFRETQDIMLRRKEFDRATNARALLEDVIPDHHL  1168

1169  YEQTNSIFRTHRKLVQDVMLPSFIQKVAGPCFHSNIMRLVRVWELKAEIANGSPFLATQD  1348

1349  IQGAVLDAVYTFAFGEYYKSSTTLPNIEILEKLNNSQNSKSDKPVDFPDVAFDD  1510

1511  LITATIDLAKAPQGVQSSPIAKVQSKVLMNMPYFRRVRKIRDDFLRGSLRSAVSKLQSEN  1690

1691  GLSDSPPVTSALEQMVLRETLLAKAENRSPNYLSTMMQGE  (0) 1810

1873  LFGLILGGYDTTSTTTLWGLKFLTGNAGAQKRLRQALQSAFPKAKSENRSPTFQELAAAR  2052

2053  IPYLEAVIEESLRCGGATSALQRLAMVDTQILGHHIPKGTEILFLTHGPSVFTPGLEIDE  2232

2233  RKRSQTCQVAGEKKDSAWDDHDIGAFKPERWLRQKESSTGTDATEAAEEFDGLAGP  2400

2401  TLAFGLGTRGCFGRRLGHQQLRTSLAILIWNFELLPCPKELSSYRAIEGLTSMPEHSYIS  2580

2581  LAKVDLT  2601

 

 >CYP526B1 N. crassa AABX01000480.1 cont3.625 NCU09636.1 (version3)

5049 MLSVVQQSIPFFDNIFNLAKPLLVLLTVTVTLYSLYRWLLPKPIPGIPYN

PQAAKSLLGDAPAMIKEVSATGEFRVWCAKQMRQMNSPICQIFIRPFSKP

WVLLADFREAKDILMRRKEFDKSSFLSDGMACMGSFHGIYMTGEKFKANR

QLIQDLMTTSFLNNHVGPAIFGKGLELMKLFETKMLLARGRPFSVKKDFE

YTSLDVMLEFAFGKNWVHTSLGPQMELVGKMQAEDIDAMMEGKDSDEPVV

FPLVPLVDFLNSVYEAPEIVEKTINALMPKLQTWWWSKQSWYKHIFDAKE

RAMKEQVEIAISNFQSGCIQTGIEHMLMREASRAEKECRAPDFRSKVLRDE (0) 6101

6163 MFGNIVGGHHTTSGAMMWLTKYLTDHPVVQSNLRSVLYTTLSAAKNENR

LFTFEEIRHAKLPYLDAIIEEMLRINAVPVTREALTDTTILGYPIKKGTQ

VFFMSNGPGFLSPSFPIDESRRSETSRASKINATWDETQDLAAFVPERWL

VRKREGNGLLADDVDFDGAAGPQLVFGLGPRTCWGRRLAYMEMRIVISML

VWNFELLKTPPALSSYAGLEGIARVPQQCHVRLRKL* 6870

 

>CYP526C1 Magnaporthe grisea MG01353.4  39% to 526B1, 39% to MG07551.4

AACU01000036 cont2.245

MALPIAVAAPVVDRLPILTILVGSGLLYLVYRWLMPKPIPGIPYSPEHTR

SIMGSLPSFLEFVKENQGRSKPWFPEQNVKLGSALVQIWETPFSRPSCVL

TDFQEAQDILMRRTKDFDRGMRDSIVFQGTGKYHHISQTSQSPI FKANKA

LVKDLMSPRFLADVSAPEIHSKVQLLLRMWSKKTQLAKGKPFNARPDIIE

AALDMINAAAFAYDEDSCSVSRQIRHLDSPDVRIDEKRDGSVDFSRAPDT

EQAVVVHELGVYLGTQFVSPVPRLAHAFNYLTRPRLRRNLAKKEEIVFTE

IRRALKRIEAGESTTA SALEHLLRREMESARKAGREPNFFTPSIRDEGWG

YIVAGHETSATAISWQVKLLADHPEVQTKLRAVLRAAYSDAAASNRLPTA

REITSTPIPYLDAFLEEALRVRGPVLMTGREAKRDAYVLGHLVPKGTFVF

MPSMGPTFHAPGIPIDDSVRSETSRTKSWGGSWDPEDVGSFRVERWLTRN

PETGEDEYDVQSGPILAFGLGPRSCFGKRLAYLEMRIVIALLVWSFEFQK

LPEHLSSYAAHDTITTGPTCCYVGLKELN*

 

>CYP526D1 Magnaporthe grisea MG07551.4  poor match 39% to MG01353.4

AACU01000913 cont2.1400

MPSIPSTHTLISGALPGYVGLAAAISVFILAVLYRLALPKPYPGIPHDKR

AARSIFGNLTEMLDYLAKNENRITLWFAEYQQRVGSPLTQYFPAPFSKPL

LLLTDFLETQDIQLRRTKEFGRGKSVANIFRYTTPEFHVALSEHDSRYKA

NKALVKDSMSPAYLNNLAAPCIYDCAMDLVNHWKFKIPLAGGRPFSAHDD

IFGTISDMILASLFAFNSGIRSTQQQLKYLESLGADVQTRDAEGQGIDFP

KLDHLPYFRAFEILAEHQSDQAKTPFKVLQHYYSMLTRPELRSCYGTIKQ

FIKTEIDKAIMRLEHDNTITCALDWMVTREQQAAAKEDRKPEYHKPAFYD

GISGYYAAGHETSATTSSWALKFLSSYAQVQDRLRTELRKSHAAACQEGR

NPTIEEITRTSVPYLNAVIDETLRHRSPLQPSAREALVDTVVLGHRVPKG

ATIMMPYLGQSLLTPAFPVPLHLRSETFPKEGGRKAWADEDIQEFIPERW

LRVDEEGREVYDAAAGPMSAFGFGPRQCFGKRLAYLQIQVVLTLLIWNLE

FQPVEGTLGSFETIELTAIQPKHTYVKLKLAQ*

 

>CYP526E1 Fusarium graminearum FG03860.1 AACM01000167 FGcontig1.167_scaffold2

MSSHLVDLYEQAPKSFILTLFLSIASTLYLVRRALLPKPIPGIPYNKNAI

NSIMGDMAEFRAAKNRREWWALQAVKHQSPLVQVFMKPFGLPWVFVADHF

EASDICMRRLKEFDRADITRQQFDGLTPGHQITLKTSDPQFKKNRELVRD

LMSSSFLQQATAPQIHHKFSTLMSLWDRKRDLSGGRPFDISHDIHNAAFD

IILGASFGVDSGQGQIGKELDELRSKTTTGGNDDAFEFERVPFYDEELGC

FDTLIESFNVSIRSPYPLFSHFLYRNLSPKMREANAMRAKLQDREIAKSI

ERREKGHPQRCALDNMMAREDAIAEKEGRKPDYRSQAILSELLGYLVAGH

ETTSGVLRWGMKYLTKDQRVQSILREAFRTGYADALKQSRVPTMDEILKA

HIPYLDAVIEEMLRHARVAPITLREAVEDTQILGHHIPKGTTIGFLGNGP

GVMMPSIPVDPNKRSEASQAHVNKMELFDESNISQFVPERWLTTQTNEKG

EEVTVFDHNNGPQQAFGLGPRACFGKKLAYMEIKAFFTFVFWEFKLEPVK

AELATDEEMVALTRAPKNVFVKLTKVECCG*

 

>CYP526E2 Nectria haematococca

fgenesh1_pg.scaffold_25000035

Necha1/scaffold_25:100712-102551

52% to e_gw1.8.241.1, 60% to 526E1 F. gram.

This gene model seems correct

MLQNTIDLFRQAPPGLGLAVCAVFIGLFLILLRLARPKPIPGIPHNVEAANSIFGDLPEFRAATDRREWW

VTQIIKHQSPLVQVFIRPFGLPWVFVADHWEAVDIATRRLKEFDRADETIYAFEGVAPSHHITMRSSNPQ

FKKNKELVRDLMSVNFLQD (0)

VSSPEIYDKFSLLVDLWNSKARFSNGRPFDVGKDIHNAALDIVMAASFGLG

LEHSQVAKQIRELESASSPGGQDDVFEFKHVPIDDEIGAFTILADSIAVAVRSPLPRLGHFLYRNLSPMM

RRAAAGRDRLRNREIAKSVERRESEHPQRCALDNMLAREDVIAEKEGRKPNYYSQVILSE (0)

LLGYLVGGHDTTSSALRWSCKYMTEDQRVQSLLRKALYEAYPEAAAENRVPTIAEITKNQHIAYLDAVIEECLRHARPGA

ITFRQAMVDTQILGVHIPKGTNVGLMACGPGILTPRIEVDYSKRSANSKASMEKIPAFDESNIREFLPER

WLRTQKTDSGEEETVFDPNSGPAQAFGLGPRSCFGKKLAYMELRIFFTMIFWEFKMNTVKPELRGHDESV

TLTRSPKYVYVSLEKTGPQQSV*

 

>CYP526E3 Fusarium oxysporum

68% to CYP526E1 Fusarium graminearum

FOXG_17165

Seq runs into a seq gap. One frameshift = &

MLSRLIELSERAPKDFTLALGFSILSALYLLRRWLLPKPIPGIPYNENAVKSFMGDIPEFRD

APNRREWWAQQPARHQSPIVQVF MRPFGAPWVFVADYFEASDICMRRLKEFDRSDVTWEQ

FNGVVPGHHITLKSSDPKFKKNKELIRDLMAPTFLQQ (0)

ASAPEIH

DKFGSLLKLWDRKLDLSGGRPFDIAQDIHNSALDIILGASFN &

DSGDGQIGRQLEELKAK

PASGGPDDLFEFEDVSLSDEQGCFTTLADSVGLSMRSPLSTIHHFLYRNLSPKMR

 

>CYP526E3 Fusarium verticillioides

92% to CYP526E3 Fusarium oxysporum = ortholog

70% to CYP526E1 Fusarium graminearum

FVEG_13054 revised

MLSRLIELSERAPKDFTLALGFSILGALYLLRRWLLPKPIPGIP

YNENAVKSFMGDIPEFRDAPNRREWWAQQPARHQSPIVQVF

MRPFGAPWVFVADYFESSDICMRRLKEFDRSDVTWEQFNGVVPGHHITLKSS

DPKFKKNKELIRDLMAPTFLQQASAPEIYDKFSVLLKLWDRKLDLSGG

RPFDIAQDIHNSALDIILGASFGIDSGDGQIGRQLEELKARNVSGGEDDL

FEFEDVSIDEEQSCFTTLADSVGLSMRSPLPTIHHFLYRNLSPKMR RARA

GRDRLRDREIAKSIERRESGQPQRCALDNMLAREDAIAEKEGRKPNYRSQ

TIMSELMGYLVAGHETTSAVLRWGMKYLTANQRVQSLLREAIGNAHSQAT

KDNRIPTAEEILKAHIPYLDAVIEEMLRHSRVAPVTLRQATTDTQILGRF

IPKGTTVGFLGNGPGLVMPSIPVSPEKRSEAALAHMERTQLFDDEDLAQF

VPERWLTSNVNDKGEEETVFDPQKRPAQAFGLGPRGCFGKKLAYIEIKIF

ITLFFWVFKLEPIKPELATEDEMLALTRSPKNVYVKLAKV*

 

>CYP526F1 Fusarium graminearum FG10629.1 AACM01000446 FGcontig1.446_scaffold7

MALLTFDSIKYPVLAGGLLLVGFIYTIYRSILPRPLEGIPYNVAATKRIF

GDLPEVRAYGSLTDWLATQAVKHKSPIFQAFIRPISKPWVVVTDYYEASE

ICTRRFKEFDRGSASTSIFKGVVPGSQITLQSHNPQFKKNKELVRNLMTP

TFLSEVSAPEIYDKFSRLVELWNLKLDHADGKFFAASRDIHHAALDIIVC

ASFGIDTQDTQLVKEKDQLSIEPRSENDGEISFKEVPLREELGALTVLSE

SVAKVVRTPSPPLFHFLYRNLSSQMRNAIALTRKLQRREIENGIKRRENG

EAMKCALDEISVREEATAKKEGREPYYHSQAIASELMSYLIGGHETTSST

IRWGISYLNRDQRAQSELRKGLQEAYQQAKAEKRPPTLTEIIQTRVPYLD

AVIDETLRVSYTFGMTLREAVVDTQLLGAHIPKGTAIAFLSNGPGVLSPP

IQYDESRSSEWVRARKPKQPVDQEYAFPDFVPERWLKSVVNEAGKEEVVF

DSQAVPIQAFGQGPRGCFGKRLAYLEMKIFFTLIIWTFEFLTLPAHVATP

KEVSSLTRHPDRVFIKPRRVVL*

 

>CYP526F2 Nectria haematococca

e_gw1.8.241.1

Necha1/scaffold_8:592224-594069

58% to 526F1, INATGG missing in CYP526F1 AACM01000446 FGcontig1.446_scaffold7

This gene model seems correct

MSWPEVTSSLPLQLLVGSSVLVAVIYTFRDAFYPRPLDFIPYNAEAKERILGDLPAIKAVGSVTNWLATQ

PTKHQSPLFQAFVAPFGKPWVVVADHHEAADICMHRLKEFDRARVTISVFGGIIPGGQLTLKSSDPQFRR

NKELVKDLMTPSFLAE (0)

ISAPEIYDKFTRLLKLWECKAILSKGRPFGASREIHHAALDIIVCVAFGLDPDQ

TQLVRALEALDVDHESEINATGGPDDEFVFKEEPLAEELEAFTRMSDSVSMSIRSPSPRLFHFLYGNFSS

VMRNARRLATRLQQREIANGLERRRTGQAQRCAVDQILAREEAMAEKEGRKPDYYSQTITSE (0)

LMTYLIGG

HETTSSALRWGISYLSSDQRVQARLREALDLAHPQARAEKRLPSLEEIIQARVPYLDAVVEEVLRHSRPL

AMCMREAQTDTQILGAHIPKGTTVVFLANGPSVFTPRIQFDESRQSEWATSRREKHPVNDEIDIGAFSPE

RWLRVEKHEDGTEETVFDANLFPIQAFGLGPRACFGRRLAYREMKIFFTLITWKFELLSLPDALAVPKEV

FSLTRNPKEVYVKLRSRIY*

 

>CYP526F3 Fusarium oxysporum

69% to CYP526F1

FOXG_04849

MSLLDALDSSSTRLLLGCLLLAGIAYTLYRSMLPRPLADIPYNVSATNRILGDLPDVKAFGSLTDWLATQAIKYQSPLFQ

AFIRPFGKPWVVVADHYEAAEICTHRVKEFDRGTSSTAVFNCVVPGAHITLTSSDPQFRKNKELVRNLMTPSFLSEVSAP

EIYDKFSRLVELWNMKTEHANSQFFSASKDVHNAALDIIVCAAFGLDTQHTQLVKEIQELTPDNNISSTKIDGEIAFEEL

PLNEELNALATVAESVAKIIKTPSPPLFHWMYRSFSATLKNAFALTKQLQEREIANGIKRRANGEAMKCALDEISVREEA

LAKKEGREPDYYSQVITSEMMSYLIGGHETTSSAVRWGLSYLSAEQRAQSELRNALQQAYPQAKSERRAPSLVEIIQTRV

PYLDAVVEEILRLSYPFGMTLREVQVDTQILGARVPKGTTVVFLSNGPSILSPPIDFDDSRSSEWVKSRKPKPLEKDYDY

SSFKPERWLKTAAGPDGKEDVSFDSQAFPIQAFGLGPRGCFGRRMSYLEMKIFFTLVVWTFELLPLPADVARPKPVSSLT

RNPDRVFIKPRKVVL*

 

>CYP526F3 Fusarium verticilloides

93% to CYP526F3 Fusarium oxysporum

no gene model

MSLLDALDSSSTRILLGCLLLAGIAYTLYRSMLPRPLADIPYNVSATNRILGDLPDVKAF

GSLTDWLATQAVQHQSPLFQAFIRPFGKPWVVVADHYEAAEICTHRVKEFDRGTSSTAIF

HCVVPRAHITLTSSDPQFRKNKELVRNLMTPSFLSE (0

VSAPEIYDKFSRLIELWGMKTEYANGQFFSASKDVHNAALDIIVCAAFGLDTQHTQLV

KEIQELTPDNDTTSSKTDGEVAFKELPLNEELNALATVAESVAKIIKTPSPPLFHWIYRT

FSAKLKNAFVLTKQLQDREIANGIKRRANGEAMKCALDEISVREEALAKKEGRAPDYYSQVITSE (0)

LMTYLIGGHETTSSAVRWGLSYLSVEQRAQSELRNALQQAYPQAKLERRPPSLSE

ILQTRVPYLDAAIEEILRLSYPFGMTLREVQVDTQILGARVPKGTTVVFLSNGPSILSPP

IDFDDSRSSEWVKSRKPRPLEKDYDYSSFKPERWLKTASGLDGKEEVSFDSQAFPIQAFG

LGPRGCFGRRMSYLEMKIFFTLVIWTFELLPLPGDVAKPKPVSSLTRNPDRVFIKPRKVVL

 

>CYP526G1P Fusarium oxysporum

40% to CYP526F1, first exon is about 6000 bp upstream and second intron boundary is defective. probable CYP526 pseudogene

FOXG_12987

MSWFELTAGALSIFLLLFCLYNLALPKPIPGIPYNKSATKRLLGDLPDLIEYQKHTEEQCRWFALQNQKFNSPVCQVFIRPI (1)

RKPRVVVSDFREAHDVLSKRLKDFDRSDRAREAFAGIVPHQMLSYQTVDPKFKKHRELMRDLMSPKFLN* (0)

VSAPEIYSKVYCLVKLWDEKCLYADGRPFDAKRDIQRATLDVVTTVSFGLDEENSATKRQLDD

MLSRSTAGLIFTEPEKKIVVFPVYPLTTELHATLTIVESATVGFSSPIPRWHYWSLSQFPYMKNAARVREEWTRREIDKA

VKAISRNVPENAWATGSALEFMVLREAAAAKKAQRPPRFHRRRMYDELFGFIIAGHDTTAATLSWGVRCIADDPQAHRKL

QSLLQNAFSTALAEHRQPTIDEITSASIPYLDAVIEETLRLSAVFPIVSREAMVDTMILGHAIPKGTSVFFTLNGPSLMK

PPIGIPENIWSESLQGTKSRYGEWRAEDVEQFIPERWIDVDDKGVETYNAMKGPFLMFGSGPRGCYGRRLAYLQLRIFWV

LLMWNFEFLPVDPELRTQDVVEHVGVEPRESYAE*

 

>CYP526G1 Fusarium verticilloides

78% to CYP526-un1 Fusarium oxysporum, 40% to 526E2

FVEG_11760

MSFILYLSSMSWIELSAGGLSIVLLLFCLYNLALPKPIPGIPYNKNATKRLLGDLPDLIEYQ

KKNGQQRKWFRLQNQKLNSAISQVFVRPFGKPRIVLTDFREMHDILGKRFKEFDRSDRAREA

FAGIIPHQMLSMQTVDPRFKKHRELMKDLMTPKFLHQVR (0)

NSAPEINRKVNTLVKLWHEKCVHANELPFDGKRDIQRATLDVITATSFGLDDENSATKRQLDDM

LARSNAGVKFTQPGKKVVDFPIHPLTKELDATLTIVESSAVGFSSPMPRWHYWILSQFPY

LKNAARIREEWTRREIDKAVAAIPKDGSENTLAMRSALEFMVLREAAAAQKAGRPPRFHR

RRIYDE (0)

LFGFIIAGHDTTAATLEWGVRYIADAPHAQAKLRSALQNAFSTALAEHRQPTIDEITSTS

IPYLDAVIEETLRHSVVLPIVSRVAMKDTTILGHAIPKGTFVFFILNGPSLMKPAIEVPE

TARSEASQGTKNRYGEWRADDIEKFLPERWIDVDDKGVETFNALKGPFMTFGSGPRGCYG

RRLAYLQLRIFWVLLIWNFEFLPIDPELRTQDVVEHVGVEPCQSYVRLRKVAL*

 

>CYP526H1 Aspergillus terreus

38% to CYP526A1, 13 of 17 top hits are CYP526 members. Three others are CYP5134 members.

ATEG_03457.1

MVITLPVRASQSPSQREIVWYILSCLFAWLLYKFSLLCLPTPFHDIPHNEGTTRTIFGDLASMLSEVSKSNDIFTWLLRQ

TQKANSPLYQVFIRPFGRPSILLSDYWEATDIVLHRSREFDRTPYTADVFSGVLPNHHIHMPTGPEWKAHRRLLQDLMTP

NFLNNTAAPAIYDRALELVDLWDAKARLAAGRPFFAAEDIHNTALDAVLSFSFGGAFPHSATRPQLELLSTLYMEAVHRN

DTPLDRPAEFPQASPPDCIKAILDITGAFEYTKSSIFPRLMWFLASKTPRVRHAVRTKDNFVLRELQGATHRLVWKDSDA

DHTWAQSAVDLIVDRERKYAEREGRSPNFFGSAIRDEVFGFVLAGHDTTSTTIAWGIKLLADNPDAQTNLRLALKNALLE

AAASNRLPLCSEVIRTSIPYLDATIEEILRCAPTVPAVDRVALNDTVLLGHSIPKGTSIFLMNKGASFFSPPHEIEDEKR

SPSARAAKNKGHHAWDPETLDIFLPERWLVASEEQASAVGTANVVFDPAAGPAMPFGGGPRGCWGRRLAYLEMRLIFALM

VWRFELLGCPKELSGYDAFDGLVHKPKDCFVRLKRIES*

 

>CYP527A1 N. crassa AABX01000356.1 cont3.332 NCU05848.1 (version3)

4191 MLQELLSLAQGPYALPILAASLVAYLLVSITITYRRLSHFPGPFLASFSP

LFMLKVLFSGHHADGYSRLNQQYQSPLVRIGTTDLITDDPAIIRHMNGAR

SAWGRSNWYRAMTLDPRGGSLFDEPNTKTHDLFKARLSFGYSGRENPGLE

GDIDEVIGMLIQYIKSRYISDDERGVLKRMDLAKVTQFFTLDVITRIAYG

KEFGWLKTDKDQFEWTSTVIKAVPANSLIAELPLLQKIFLSKWFLQWFGP

KHSDANGMGRVMGMAREMVAKRFGEKAEDRKDMLGSFVRHGIEQQACEVE

VLFQILAGSDTTAVAIRSTFFHLATSKKAYVRLLEEIDRAVKEGEVSSPI

RAEEAKHLEYLQACVYEGLRMQAPFSGLCMKSPPKGGDFINGMFIPEGTR

VGHNFGGLIRRRDVFGEDADVFRPERWLNAEPAKRLEMQQTTEMVFGYGR

WACLGKPVALMELSKVFFE (0) 2785

2725 FFRRFDLQLVEPKNPWHEFNVNMCFQSDLWVKITERFPEGEKV* 2594

 

>CYP527B1 N. crassa AABX01000074.1 cont3.486 NCU08112.1 (version3)

26652 MASLGGFFDPSRLTTASPKAILITTFSLLIVYYITSSIIAWYRLRHFDGP

      WLGKFSYLWIFKIIHSGQMGEAAHAAQDKYGGVEPGYPSTVRIGPSDLIT

      TELDLIRRMSGARSRYTRSDWYKLNRLDPYDDNMFSTTSTTYHDKLKSKM

      APGYAGRDNTGVEKDIAHVISKLVDKIRTKYAAPKGSTSPAGREMLEFGQ

      MAQYFTLDVICKTAFQVEFDHITKEEDVFGYIYMVHNLVYGNAMGATIPI

      IGKVLSNPLFLKMFGPTTKDPRGMGLIMK (2) 27488

27541 VAREIVAKRFAPDAKDQQDML (0) 27603

27668 GSFIRRGLTQRQCETESIFQILAGSDTTATGIRSGLLYLCSNPRAYTRLQ

      REIDERIASGLISSPCITNAESLAFPYLQAVIYESLRMRPPFDGLPFKIV

      PPEGDYTRDGKFIPGGTKITATFGAMQRNKEVFGVDSDIWRPERWLEEEG

      CDAERRREMKSVVEMVFGYGRWQCAGKMVAMLELNKIFCQ (0) 28237

28368 LMRNFDFQLANPTQPWKSRNYHIFFHDDFYLTVTDRHSKTEDEKSS* 28518

 

>CYP527C1 Magnaporthe grisea ESTs BM871448.2, BM863551.2, BU644850.2

MG07626.4 AACU01000923 cont2.1411

MMLQQVADALATHWLSGILAIATVYLATSYIIDYRRLR

AFPGPPLGSFSYLWLAYNALQGRQGSIFYEVMKRYRVPEHSFVRIGPNDLMTDSPEVVRHMS

SARSTYLRSSWYRTSKLDPSGDSLLSIMDTAHHDALKAKAGRGYAGRDNRNLESDIDDQL

RRLIGLLERKYLSGGGDASSFRPVDMATTMQYFTLDSITKLAYSSAFGFLDLDTDVYGYI

KAIRDAAPPIIVCSEWPLAGRIFFSPPFLKMFGPTPKDKSGVGKLMGTLRQVVASRFGPDA

KDQPDMLGSFVRNGLSQHQCEQEVILQIVAGSDTTATALRGTLLQLCSTPMVYLKLQKEI

DEAVRSGMVGEGVISQETARKLPYLQAVIYEGLRLNPPFTGALMKEVPPGGDEIDGVFIPAG

VRIGVSAKGIQMRQDVYGHDVDVFRPERWTECDEQRRMRMAANTELVFGYGRWMCAGKNVAFME

LNKVYFELLRRFDFQVVDTKTPVKEESFNVMVSKDMFMKVTKRVLYTGSILIRAR*

 

>CYP527D1    Botrytis cinerea = Botryotinia fuckeliana

             GenEMBL AJ609392.2

             join(<935..1716,1765..1828,1878..2111,2195..2515,

             2575..2703)

             aba1 gene

             43% to 527E1, 43% to 527A1, 41% to 527B1, 41% to 527C1

MSNSILNLGSFACLLSLGSIVLWYTISAVLAWYPLRKIPAPSFL

ATFSYLWLAKTTYSGKQYWIQRDLHKKYGPLVRIGPTDIITDDPEIIKKISSARSSHR

RGDWYLTGRFNPYYDNMFTMLEPGPHAKAKARTAAAYSGRDMPDLEVGVNAQLQTLIG

LMRSKYASNTVKPHQPLLDLGQVSCFFTMDVITRLAFGEEFGYLKEETDQYGFLGEVR

ELWPRMSTSADTPWIRKFLFSPPFLKVLGPKPTDKTGFGALMAVAEHHVGKRFAPDAK

KKEDMLGSFIRHGLNQQECEVEGLFMIVAGTESTASAIRSTLVHVMTCPRVYQKLKTE

INLAVEEGKVSSPIKLEEAKLLPFLQAVIYEGIRMRPPLLGLFPKIVPDGGEEFHGMF

IPAGTAICMNTSSLLRSTALFGDDAEVYRPERFMELEKSKRGEMERNVELAFGYGQYM

CVGKTVAFMELNKSIFEILRAFDLQLLSPAKPCDVLSYGIFLESNMLVKVTESEGTEYK

 

>CYP527D2 Aspergillus niger

e_gw1.21.29.1|Aspni1

59% to CYP527D1

MDFYKFATFIVCFFIGWYALTSVLAWYRLRKFPTPSWLAHFSYLWLGRLTYSGKQYWVHRELHRKYGPLVRIGPNEIMTD

DPDIIRSMSSTRSTFTRGEWYIAGRFNPHYDILFTVLGNDAHKKARSRVFEGYSGRETGIAVEHGMDRQVTRLIDLIRQK

YAVPGQDAPLLNIVDISSYFTMDVITELAFGQEFGYLQAEEDRYGFFHEVHSLWPQMSTVADVPWIRRIVFSKPFLKLLG

PRTTDSHGFGALMRVAHEHVQQKFSTLDNSKGDMMESFIRHGLTQEECEAEGLFTIIAGTESTAGTIRSILIHILSSPVV

YSKLKNEIHDAVQKGPISSPISNKEVRDLPYLQAVIQESIRMRPPLLGLFPKVVPAGGVEYHGQFIPGGTHICMNTSSLL

HSKSLFGNDPSVFRPERFMEASEDTCSERRKNLELAFGHGQNQCLGKTIAIMELSKVTFEMFRNFDFQTVSPWRVNHQVQ

SYGVFLESNMKFRVSQSE

 

>CYP527E1 Fusarium graminearum FG01767.1 AACM01000092 FGcontig1.92_scaffold1

MDLNSLSNYKTPGGAVVVLLAFVTFWWLTTTLIAWNRLKHIPGPWVAGFT

YAWAGWTEYSGKQHFSFLDLETKYGSLVRIGPELLATSDVEVVRRMSARK

STYRKSSWVDGVRLNPYHETMFGVRDPIEHDRIKTRLAPTYSGRDTPHLE

SVVDQQVNNLVSLIRRKYVSDPTSGDFRVMSLIKVASYLTLDVISKVGLG

TEFGCCASDSDPYGFTKAVAEHMPLMAMTSGVPWMRNVFCSPLFLKYFGP

KDTDTYGLGPLIKVTNDNVRARYSQEDGGKRDMLSSLKASGLPEGEAQAE

TLFLFIAGSDTTAAAIRVTMFYIISSPRVYQKLKKEIRKAIHEGRASSPI

TVAQARELPYLQAVIYEGLRIRPVTTGPQSKEVPPGGDTINGHFIPEGTS

IAINFPAIMGSKKLFGPDADVFRPERFTDLQDPDLAEMRRNVEMNFGNGR

WMCAGKPIAFMELHKVYFELLRAFDFQLVEPLKPMTSESYSLFRDHGLNV

RVTLAEDME*

 

>CYP527E2 Fusarium oxysporum

70% to CYP527E1   Fusarium graminearum

FOXG_13007 revised

MTLSYLSEFPTSSGIGIAVLCLLSSWWLGTTFIAWYRLRHVPGPWFAGLSYIWNGWIAYSGKQHHIFVALDEKYGSLVRI

GPEVLLTSDVELVKRMSATKSSYGKSSWVDGVRFNPYHETMFAVRDPRQHDRAKARVAPAYSGRDTPNLEGAVDQQINSL

ISLIRRKYLSEPASGAIRTLPLLNVLSYFTLDVISKVALGTEFGCCTSDSDPYRFYEGLDEHMPLMALTSDVPWIRAVMY

STTFLKYFGPRETDTHGIGPLMK (2)

VTNDNVRRRYSQQDQTEKTDML (0)

SSFKVHGLSEGNAQSETLFMFVAGSDTTAAAIRVTLFYLMSCPRVYR

KLKEEIRGAIREGRASSPITAAQARELPYLQAVIYEGLRMRPVTTGQQAKEVPAGGDTINGHFIPGGTSIAINFSAILGS

KALFGPDADVFRPERFIGLSASNLAEMRRNVEMNFGHGRWMC

AGKPLAFMELQKVYFELLRAFDFQLTEPLSPMRSESYA

LFRDFGLKVRATVAEDME*

 

>CYP527E2 Fusarium verticillioides

92% to CYP527E2 Fusarium oxysporum = ortholog

FVEG_11734 revised

MDFSYISDLSSSSGIGIAAFCLLSSWGFITTFLAWYRPSHVPGPWFAGL

SYIWIGRTAYSGKQHHVFVALDEKYGSLVRIGPEVLLTSDVELVKRMSATK

SSYGKSSWVDGVRFNPYHETMFAVRDPRQHDRAKARLAPAYSGRDTPNLE

GAVDQQVNNLISLIRRRYLSEPAAGVFRTLPLLNVLSYFTLDVISKVALG

TEFGCCTSDSDPYRFYEGLDEHMPLMALTSDVPWIRAIMYSTTFLKYFGP

RETDDHGIGPLMKVTNDNVRQRYSQDQTEKTDMLSSFKVHGLSEGNAQSE

TLFMFVAGSDTTASAIRMTLFYLMSSPRVYRKLKEEIRDAIRDGRASSPI

TAAQARELPYLQAVIYEGLRMRPVTTGQQAKEVPAGGDTINGYFIPGGTS

IAINFSAILGSKALFGPDADVFRPERFIGLSASDLAEMRRNVEMNFGHGRWMC

AGKPLAFMELQKVYFE (0)

LLRAFDFQLTEPLSPMRSESYALFRDFGLKVRATAAEDMEQLSE*

 

>CYP527F1 Fusarium oxysporum

51% to CYP527E1 Fusarium graminearum

FOXG_09111

MASPFFERGLTWLPYFIVALFTWLLLSSAISWYRLRHIPGPFFGKLSYLWVAYITITGTQHDHLVN

LNRSYGPIVRVGPNEVLVDDPDLIRKVSSTRNTYTKGNWYLGGKFNPYHDP

MFQITDPAQHDRVKAKLSPAYSGRDAPNLEAVVNRQIGSLIQLIKEKYTSAQGDYRPMEGTRVTRLFALDVISNLSLGQE

FGYLKADSDFHGIVKALNEHMLV MTLATDIPWLRNLIFSPMFLKLFGPTENDTKGIGPLMKVANTIVRERYAPEADEQHDMLA (0)

GSFKRHGLRLEECQAESLFMFVAGSETTATVIRVILLYIVASPHIYQ RLRQEITEAISEGR

ASSPITDVESRQLSYLQ (0)

AVIYEGIRIRPATLGMFFKDVPAGGETMHGKYIPAGTSIGINASSLLRSE

ALFGPDPQVFRPERYLEVDKETSAQMKRDVEIVFGYGRWMCAGKPIAFMELNKVIFE (0)

LLRVFDFQLVEPEVAMKSESYMVFHDQSLILRVTEADHK*

 

>CYP527F1 Fusarium verticillioides

89% to CYP527F1 Fusarium oxysporum = ortholog, one frameshift

FVEG_06713

MASPFLERGLVWLPYFIASLFTWLIVSSIVSWYRLRHIPGPFAGKFSYLWVAYITITGTQ

HDHLVNLNRRYGPIVRVGPNEVLVDDPDLVRRVSSTKNTYTKGNWYLGGKFNPYHDPMFQ

ITDPAQHDRLKSKLSPAYSGRDAPSLEAVVNRQVGNLIQLIKEKYISTQGDFRPM &

EGTRVSHLFTLDVISNLSLGREFGYLKADSDFHGIVKALNEHILIMTLATDIPWLRNLIF

SPMFLKLFGPTENDTKGIGPLMKVANTIVRDRYAPEADDQRDML (0)

GSFKRHGLRLEECQAESLFMFLAGSETTASVMRVILLYVIASPHIYQ

RLKQEMAEAISQGRASSPITDVDSRQLPYLQGVIYEGIRIR

PATTGMFFKDVPAGGETMHGKYIPPGTSIGINASALLRSEALFGPDPEVFRPERYLEVDKETSAQMKRDVEIVFGYGRWMCAGKPIAFMELNKVIFELLR

VFDFQLVEPGVAMKSESYMVFHDERLVLRVTETVHEQG

 

>CYP527F2 Fusarium oxysporum

52% to CYP527F1 Fusarium oxysporum

FOXG_15185

MLLEHFTANYWWAAALAIIAWYIVSSVISWYRLRHIPGPLLAKFSYLWLARLALSGKQYETHLELNKKYGPIVRVGPYEV

LTDDLELLRKVNGTKSLYAKGASYSGSRLNPWHESLFMMRDPIAHDKMKAKLIYGYSGKETMGPEAAIDEQIMNLVRLIQ

DKYISKPREFRPLLWSKTAGLFTLDVISRLALGQEFGCLNRDEDIYCFFDTLRDYLPIVSLTTDVPWLRNIAFSPLFLRL

FGPSIKDKKGIGKMMGLTNKVVEEFYHSDGEVRRDMLS

SFKKHGLTKDQCEIESIFMFVAGSDTTASVIRAGMLHILATPHVYCRLKREIANTIREG

RASKPISNADSLFQPYIQ (0)

AIVYECLRIRSVSTNMSFKEVPTGGDVYNGKFLPAGTNIGMNFSGLLRSETLFGKDAHIFRPERFTEVDDKTSAKLKRD

VELTFGLGRWSCLGKPIALMELNKIFFE (0)

LFRHFDFQLAEPHKAMQFDSYMLFRDKGLVLRVTESDLKDLKSIKEDIY*

 

>CYP527F2 Fusarium verticillioides

90% to CYP527F2 Fusarium oxysporum = ortholog

FVEG_12563

MLLEHFTSNHWYAAALAIFTWYILSSIIAWYRLRHIPGPFLAKFSYLW

LARLALSGKQYETHLELNKKYGPIVRVGPYEVFTDDLELLRKINSTKSSYAK

GASYSGSRLNPWHESLFMMRDPVAHDKLKAKLIYGYSGKETAGPEAAIDE

QIMNLIHLIQDKYISKPKEFRPLVWSKTAGLFTLDVISRLALGQEFGCLK

RDEDIHCFFDTLNDYLPVVALTTDVPWLRNIAFSPFFLQLFGPSIKDKKG

IGKMMGLTNKVVEEFYHGDGQVKRDMLSSFKKHGLTKDECEIEAIFMFVA

GSDTTASVIRAGMLYILATPHVYSRLKREIANTIREGRASRPISNAESLS

QPYIQAVVYECLRIRSVSTNMSFKEVPAGGEFYNGAFLPAGTNIGVNFSG

LLRSETLFGKDAHIFRPERFIEVDDKTSAKMKRDVELTFGLGRWSCLGKP

IALMELNKIFFELFRHFDFQLAEPHQAMLFDSYMLFRDKGLVLRVTESDLEDL*

 

>CYP528A1 N. crassa AABX01000356.1 cont3.332 NCU05849.1 revised

8219 MSLPTLQDVLATSISLLKTTTPLLKRITSLLLKTTTSLLFIIVVTILAIA

     VYRLFFHPLAGIPGPRLAALSNIWHAYHARNGRM YLLGKSLHKRYGPVVR

     VGPNELWFDSKEAFKTIY (1) 7866

7779 SAGSGFEKSDFY (1) 7744

7659 LSTLLTKPTLSLKFSPFKFP

     FPNLQLPLQIHHPDTLDLLSERHLPRYRLQRRLIGPLYQLSSLKKFEPQI

     DAVLDRAIAQLRTLQGQEVDLKEWMHIIAVECLGAVVLSWSPGYIRDKSD

     GGTSVQGYLGWKRKSVFGLFPGITILSFLEG (1) 7207

7173 GVARWWSNTWGVTFATPKGFKPFFTAVYQKVSRRVSMAALLWMSDE (0) 7066

6953 MEEDKGKKDLLEDLIKLYQSKPEQFTETYLRRMAMTNFGAGHET

     LCSALTACMAMIGTHAAVQARVAEEVGSHLRGGGTVPFDTAAKQLPYTLA

     AIKEAQRLHPVIAMSLSRTVPRDLDGGGVMLHGHWVPEGTT

     VGCNPVALHRNQEIFGEDADVYNPSRWLP (1) 6490

6154 LERKRLREMLHLNLTYGGGARTCPGRHLAELIVWKVIPRLVAE

     FRVEVTHMPRDEEKNMERYFMSMLTGVKVRFLERREDSGDMA* 5897

 

>CYP528A2 Fusarium graminearum FG02458.1 AACM01000122 FGcontig1.122_scaffold1

142355 MMTLGSLLVSVFTR

142397 IICTLLVLVASIAIYRLYFHPLAHVPGPRFAAISSVWHAYNARNGRMAYLGKTLHRRYGP 142576

142577 VVRVGPNEVWFNTKEAFHAIY (1) 142636

142689 SSGSGYEKSDFY (1) 142724

142772 LATALNKPYIDWHLNPEFPDTLDLLSERDVRRYRIQ

RRLIGPVYQTSSLIQYEAAVDEVLARTVAKLKSLKGAQVELNEWMHIIAVECLGAIVLSWSPSMLKNGTD

NGSGTHAYHGWRRKSVFGLFPLAAKLEFLSKSTGRLFSTIWGVNFQPPKDFRPFFP (0) 143257

143308 DVGKRASRRINAATKSKHHKDDRQDLLKDLISLHKSKPEFTVNYLRKMVITNFGAGHETM

ASTLTSIIAMLGSNNDIQEQVSREILETHNPADYSTATRLPKTQCLIKETKRLYPVIS

MSLPRTVPAGGLHLHNNYFPPNTTVGCNPVALHRNPEIFGSDCNEFKPDRWLTADSDA

ARNMERVSLSWGGGARTCPGRHLAELVVFKVIPALIKEFSIEAIVPPEDENRSYFLSM

LTSVKSDS* 144033

 

>CYP528A3 Magnaporthe grisea MG03500.4  49% to 528A1 AACU01000719.1 cont2.687

54% to AACM01000122.1 Gibberella zeae

MTFFWSLVVVATPALLVLAVAVYRLFFHSLAHIPGPRLAALSNVWYG

RQVCSGRMLQVGKILHRQYGPIVRVGPNEVWLDSEEGFRQVY

 (1) SAGSGFEKSDFYL () 58238

58157 STALQKPKVDWRLQPHFPDTLDLLSERDMR

RYRLQRRLIGQVYQPANLRKF

EPVIDDVLDQVVAQIRTLGDAEVDLKEWMHIITVECLGAIVLSWSPGYLR

AKSDFGSGSFSYLGWKRKTVLG () 57789

57700 LFPLATVIGVLSRSFERQFANIWGLTFKAPKTLKHFFP () 57587

57513 AVHRKVKPRLVNMS

REKPRKDARRDLMADLVQLHKERPEF

NGTYLHRMAITNFGAGHETTTSALTSVLAMLGTHLDIQARMADELVNSRG

TENEMASKDSSPRMPLTQAAIKEAQRLYPVIGMALPRRVPDGGVRIGDDF

LPAGTTVGCNPVSLHRNKDIFGPDADIYNPERWLTDDKRAKTMERNNLTW

GGGARTCPGKNLAEIILYKTIERLVHDFEVVCKLPPDEEICYYFMAMLTGVK 56788

HFDCLRAKEWTTAG*

 

>CYP528A4 Nectria haematococca

e_gw1.18.26.1

Necha1/scaffold_18:578149-579878

74% to 528A2 F. gram. ends don�t match, 528A2 may have errors

MTLLCSLIAHVLLKTVFTLVVLLASIVVYRSTFHPLARVPGPKLAAITSFWHAYHARNGRMAYLGKTLHS

RYGPVVRVGPNEVWFDTKEAFQAIY(1)

SSGSGYEKSDFY (1)

LATSLSKPRIDWKLNPHFPDTLDLLSERDVRRYRIQ RRIIGPVYQTSNLIQ

YEAAIDDVIERAIAKLKSFKGAPVELNEWMHIIAVECLGSVVLSWSPGMLKNGTDWGSGSHAYHGWRRKS

VFGLFPTIVKLEFLSKTVGRLFSSAWGVNFKTPKNFKPFFV (1)

DVGKRVSRRVNAAKRASPPKDTRQDLLTD

LIRLHKVRPDFTDNYLRKMAVTNFGAGHETMASTLTSIMAVLGSLQDVQTQVSQEVLSMTDPSRYSTATR

LPIMQALVKEVKRLYPVISMSLPRKVPNGGLHLHGFYFPPDTTAGCNPVALHRNQDVFGPDSHLFNIARW

LNADPDTARNMERVSLSWGGGSRSCPGRHLAELVVFKVVPALVKEFRIEAVLPPEDDGRSYFLSMLTGVK

TRFIERQAAESTTDTQKPVS*

 

>CYP528A5 Mgr017 Mycosphaerella graminicola

54% to 528A2 N-term from JGI model estExt_fgenesh1_pg.C_chr_70267|Mycgr3

cyan region does not match

MASLLNAIALVTVKAIVSVL

VLIIGIIIYRLYFHSLSNIPGPRLAGISNFWHAYHARNGRMLEIGTNLHRQYGPV

AGATQGYPKSSFY

VALALNRPSVTP

CLGLSFPDTLDLLGEFDLDRYRLQRRLIGPLYQTRSLLKHEKSINEVLLHAVARLKGLNGAPVDLKEWMHIITVECLTAV

VLSWSPGYLKAGTDHGVGPHAYLGWRRKSVFGLFPWAEVMDAYSRTLGRTFAKGWRLTYQPPAHYKALFPLLGTKVSKRI

NAALRPIPPKDGRNDLTAELIQLHKDKPEFSKDYLKRMVMTNFGAGHETTTSTLISVLTMLGLHAEAQGRVAAEISNTPR

ESQSVSYAEATDLTYTQAAIKESQRLHPVIGMSLSRRVPPAGLQVHGQYFPPGTTVGCNPISLHRNPEIFGPDAELFNPE

RWLHSSRVQDMERCNLLWGAGQRTCPGQALAKLIVFKVVATLVRHFKVEVEVPPEEEMKIYFMAMLTGVK

 

>CYP528A6 Mycosphaerella fijiensis

63% to CYP528A5 Mycosphaerella graminicola

estExt_Genewise1Plus.C_110131 revised

MCHTIGTIFTLFGVVLVTVIIHRLCFHPLHRVPGPKLAAVSNTWHAYHARNGRMMSLGRTLHATYGPV (0)

IYSATHGFEKSTFYIALALKRPELTRTLSLSFPDTLDLLGEFDMERYRLQRRLIGHIYSRRNLLKYEGTVDAVM

AGVVEHLRTLLGAEVDLKEWMHIITVECLSAIVLSWSPGYLRAGTDFGSSGHSYLGWRRKSVFGLFPTAEIMDNYSRSLG

RLFAKVWGLTFTPPANFKPFFPAVGNKCMRRINAALRSKPPNDKRRDLAYELIELHKSRPEFKSTYLKRMVMTNFGAGHE

TTTSTLIAVLSMLGSHAEAQDQVRDEVQDYARIDCYANACTLTYTQACIKEAQRLHPVIGMSLSRTVPANGTEIDGFYFP

PGTTVGCSPVALHQNPEIFGLDARDFRPDRWLKEDDAKIMDRYNLIWGGGARTCPGRALAEMIVQRVVSTLIKEFEIKVS

MPAEEDMPMFFMAMLTRVKACFRAREANPLSGG*

 

>CYP529A1 N. crassa AABX01000356.1 cont3.332 NCU05874.1 (version3)

88106 METSNLTLLLLYLAIPSLLISAVQQILSWRKRTALVGSSGSKAKFPGPRQ

      FPIVGRIHDLPRFSLWLKFKEWADEFGPIYTTSVPDATFVIISDEKIAEE

      LLVKRGHIYSGRPQIRSLIGHKEGVVYSALMDRH (1) 87705

87637 DTWKSQRKWAHAAMAA

      AYKHHFYGHVESEMRRFLVVLLFDPDRFLDHTREYCGRVMSRLAWDDATQ

      GRLNGESADKTLHCMSVSGPITNTMTPLWSLPASINPWYKYEVKREAELR

      QWWLGLFRKAKERMRKGELPSDTWAYRYFEQMVQDTAPSMPATARAPGPD

      VKNAERSPSTAAQEEAYEKKDALDQPEKDETFASCMIGFLNLVGVVTISG

      PLKFFQMAMALHPEWQHKAQAEIDEVCGDRMPTMKDFDKLPILRACLKET

      VRWRSGVPLGVPHQAERDDEFRGVKIKKGTIVLACEW (2) 86729

86660 TLNRNPQKYPDPE

      TYNPARYLDPSYPTFQAPLTRYPNFREGSSMHSFGWGRRTCLGQNIVDDE

      MFVFGAALLWAFEVTPKTCPVTGKEVPIDTHKTNSHVILEPVDFQLGYK

      VREGKKELILEGGKEFFGKV* 86262

 

>CYP529A2 Magnaporthe grisea MG00832.4  66% to 529A1 AACU01001468 cont2.149

MEQIVLLLCYLALPTIFLTVVRHALSLVQKKPNSVAKFPGPKQYPIVGRI

HDLPRFSLWLKFKEWADIHGPIYQTRAVDNTFIIISDEKIAEELLVKRGH

IYSGRPQIRALIGHKEGPVYVALMDRHDSWTQQRKWVHAAMAEAYRNRFY

GHVESETKRYLAMLLLDPSRFLCHTREHCGRVMSRLAWDQAQQGAENGRS

ADETLHCMSVSGPIVNTVSPLWQLPWALNPWWRNEVRRESVQRAWWAGNY

HVARRRFLEGDLPADTWAHRYFDQLARDGNDALEQSPEQVDFASCMLGFF

SLVGAVTISGPLKFFLMAMALHPEWQRRAREEVDRVCGDRMPTVDDFAAL

PTVRAVLKETVRWRSGVPLGVPHQAEQDDVFRGVPIKKGTIVLACEWSLN

RVPEKYPDGDSFRPERWLEPGWPTFQEPLSKYPNFRNGHGMHTFGWGRRT

CLGQNIVDDEMFVFAAAMLWGFDMAQKLDRRTGEPVAIDTQATNSHVILE

PLPFEMAFKPRSEARAAGILRGYEEVRAGLRVVD*

 

>CYP530A1 N. crassa AABX01000046.1 cont3.150 NCU02852.1 (version3)

43902 MAVITTYLPIVVGIALLIRLLMVGRRPKNYPPGPPTIPILGNLHL (0) 43768

43698 MPTKDVHLQFEKWAREYGPVYSLILGTKVMVVLSSDRAVKDLLD

KKSHMYSHRQEMYVGQTLCSGDMRILMLGYTA

KWRMSRKLVHALLNVSSAKSYVPYQMLEN

KQMLYEMITKPDEFLYNIRRYSNALTTTMVYGWRTPTYNDEKMRQLFDGF

SEFAEINQTGVAALIDFFPILRYLPDFLLPARKKAKELHKKELGLYKGHW

LKAKEVTLKGTITHCFCEDAVPVQKKEGFSDDEAAYIAGTLLEAGSDTTS

STLYGFVQAMLLYPEVQRKAQEEIDRVVGPDRLPTMDDEPHLQYCRAIVK

ESLRWMPTTIMGAVPHAVTQDDYYNGYLIPAHAAVVNNAWAINHDPVRAP

EPRKFKPERHLDDKLSLADSAANPDPTKRDQFTFGAGRRICPGIHVAERS

LFLGVARMLWGFSIAPKIGVDGKEIIPDQEKLTQGFVCMPEEYPATITPR

DERRKGIMEEEWFAAERECLDPETKQWKQGMNWVPKLSSTKV* 42205

 

>CYP530A2 Fusarium graminearum FG11389.1 AACM01000465 FGcontig1.465_scaffold9

revised, 77% to CYP530A5, 76% to 530A9

MFTLQVFFLALGAVLVRLLFTGRRPKDYPPGPPTIPILGNIHLMPKRDAH

LQFRKWADEYGPVYSLILGTKPFIVLSSAQAVKDLLDKKSGIYSDRQE

MYVGQVLGSGGLRLLMM (0)

GYGQTWRS (0)

FRKLVHSLLNVTAAK

SYVPYQDLENKQMLYELVTQPDQFLQSIRRYSNALTTTMVFGWRSPIYQD

PKLMQLFDGFADFAEINQTGIAALLDTFPILRRLPDFLLPVQKKAKELHK

KEKALYLSHWLKAKQDIANGSIKPCFCVGLAEAQEKNKFDDDQAAYISGT

LLEAGSDTTSSTLYAFVQAMLLYPDIQRRAQDEIDKVVGNAQMPNMDDES

SLQYIRACMKETLRWMPTTILGAVPHAVTQDDIYNDYLIPKGAGVLNNVW

GIHMDPERYPNPRQFNPERYLDDFQSLADAAANPDASKRDQFTFGAGRRI

CPGIHVAERSLFLGMSRILWAFNIEPALDEMGRPILPDPDKLTQGFVCMA

EEFPAKITPRSAEKRDLVIESWRNAEKELLDPVTKQWR*

 

>CYP530A3 Aspergillus nidulans AACD01000151.1 revised

revised 7/19/07

MLPLTPILAIAGLLALIFRLSLIGRRPKNYPPGPPTLPLLGNIHQMPSRD

AHLQFAKWAKEYGPIYSLMLGTQCLIILSSDEAVKELLDRRSGIYSHRQE

MYTGQQLCSGGLRMLMMGYGPTWRIMRKMVHGLLNVSTSRNYIPYQMLEN

KQMLYQFLTEPDDFLKHIRRYSNALTTTMVFGWRTPTYEDPKMKQLFDGF

SEFAEINQAGAAAFLDFFPLLQKLPDFLVPTKRKAKELHKHEMGLYKGHW

MKAKEEIEAKNIKPCFCQGMYEVQKKEGFSDDQAAYISGTLLEAGSDTTS

STLYAFVQAMLLYPEIQRKAQAEIDRVIGPDRLPVMDDLADLQYIRSCMK

ETLRWMPTTILGAVPHAVTQDDSYMGYFIPKGAGVMNNVWAIHHDEKRHP

NPRQFNPDRYKDDTQSLGDAAANPDASKRD

AFTFGAGRRICPGIHVAERSLFLGMSRILWAFNISPKKDASGKEIIPDQERLTQGFVCMP

EEFPATITPRDEKRAEMVRNEWRQAEKELDSETKQWVVAPVDIKI*

 

>CYP530A4 Nectria haematococca

e_gw1.14.490.1

Necha1/scaffold_14:351965-353894

49% to 530A2

MLSLLISVVLSFTALAVLLLAKLSKIGQRPPGLPPGPPTLPLLGNLHL (0)

MPTKRPHLQFEKWAKQYG (2)

PIYSLILGTKIMIVLSSDEAVKDLLDKRSAIYSDRPDMYIGQRIASGGLRLVLM (0)

RYGSNWRMIHRMIHKALNITVAATYLPYQDLENKVLLTNLLDTPDDLL

PHIRRASYSLSTQMIFGFRCISNQDPKLLQLFH (0)

GFDKWSALSGSASAQLADLYPALQNLPDFIAPNVSYARKLHQAEKKMYLGHWARTKQAIVDGRGL (0)

PCFCNDIYRAQLTEGFSDDAAGYISGSLLDAGSDSTASTLYGFILAMLIWPNVQKRGQEEVDRVVGPD

RLPTMEDYDQLPFIRCCIKETLRWMPTVVLGVPHNVTKDDSYLGYQIPKNSSIINNVW (2)

AIHMDETRCPEPRVFNPDRFKDIPSTLYQSVIGDVAKRDNFVFGAGRRLCQGIHIAERSLFLAISRFFWG

FNFLPTKDEYGHPVQYDAEDLVGGITVQPADFPAVIEARSKRRADIIRKEAQESEKSLDPETLQWIKVPEGMVFNNE*

 

>CYP530A5 Aspergillus fumigatus

AAHF01000014

complement(join(613367..614597,614640..614689,

614717..614932))

locus_tag="Afu8g00760" third P450 of 8 on this accession

54% to CYP530A4, 77% to 530A2, add missing first exon

79% to 530A9

revised small exon

MLPLALVGAVGALVFLVFRLLSIGRRPKNYPPGPPTLPIIGNIHQ (0)

MPTRDAHLQLQKWAQEYGPVYSLILGTKPLIVLSSDQAVKALLD

KRSALYSHRQEMYIGQTLCSGGLRMLMM (0)

GYTPVWRG (0)

FRKMVHGLLNV

TAAKSYVPYQMLENKQMMYEFLTQPERFLYSIRRYSNALTTTMVFGWRTPTYEDAKMK

QLFDGFSEFAEINQTGTAALIDFFPWLRMLPDFLLPTQKRAKELHEREKALYLGHYLR

AKQDIRDGQIKPCFCVGLAEAQKSDGFSDDQAAYISGTLLEAGSDTTSNTLYAFVQAM

LLFPDVQRKLQEEIDRVVGPRMPTMDDEQDLQYVRACMKETLRWMPTTILGAVPHAVT

QDDEYMGYLIPKGAGVVNNVWAIHMDEKRHPNPRVFNPDRYKDDRQSLAEAAANPDAT

KRDQFTFGAGRRICPGIHIAERSLFLGISRMMWAFNVEPAVDEKGQPILPDPDRLTQG

FVCMPEEFPARITPRSQAKADMVIKEWKEAEQQCLDPETKQWLLSPVQ*

 

>CYP530A5 Neosartorya fischeri

95% to CYP530A5 Aspergillus fumigatus = ortholog

NFIA_094190

MLPLAVLGAVGALIFLVFRLLSIGQRPKNYPPGPPTLPLIGNIHQMPTRDAHLQFQKWAQEYGPVYSLILGTKTMVVLSS

DQAVKDLLDKRSALYSHRQEMYIGQTLCSGGLRMLMMGYTPVWRGFRKMVHGLLNVTAAKSYVPYQMLENKQMMYEFLTQ

PERFLYSIRRYSNALTTTMVFGWRTPTYEDEKMKQLFDGFSEFAGINQTGTAALIDSFPWLRRLPDFLLPTQKRAKELHE

REKALYVGHYLRAKQDIRDGNIKPCFCVGLAEAQKSDGFSDAQAAYISGTLLEAGSDTTSSTLYAFVQAMLLFPDVQRKL

QEEIDRVVGPRMPTMDDEQDLQYVRACMKETLRWMPTTILGAVPHAVTQDDEYMGYLIPKGAGVVNNVWAINMDEKRHPN

PRVFNPDRYKDDRQTLAEAAANPDATKRDQFTFGAGRRICPGIHIAERSLFLGISRMMWAFNVEPALDEKGQPILPDADR

LTQGFVCMPEEFPARITPRSQAKADMVIKEWKEAEQQCLDPETKQWLLSPVQ*

 

>CYP530A6P pseudogene Nectria haematococca

e_gw1.10.737.1

Necha1/scaffold_10:90207-90395

37% to 530A2

PQRGSRLPLPPNPKGHQLLRNLLDLIKHAKDGNIHLLF*RWSKQDGKIFRLGSLE

SYYLNNDEAVNVLMDK*SANTSGRPRWISSSEHMTNSWNVLLLDSIKPRWKKHRKVLNSALT

 

ESFEEAAFTALALILGAADASAISTWSFLEAMLLFPNV*EKARSMVLEVTKDRTPVYEN

LHDIPYVRCLMKET*R*RPLIPLGHPHSTSEDINYEGYRIPKGAGLHLNAFAVGHQEKRN

HNPEMFNVMGRIQPHPSRA*

NLADVTKRDHFAFGPGRRACPRYHAAERSLAIAIMRILWAFEICPAED*

ELPLDPSKFEGRVMPRPPHERLPVCLRVLDEK

 

>CYP530A7P pseudogene Nectria haematococca

fgenesh1_pg.scaffold_44000004

Necha1/scaffold_44:12309-13331

43% to 530A2 EXXR motif mutated to KTFR probable pseudogene

AMVLFPEAQKRAQEEIGSIIGNGLLPEWNDRKDLPYIHGVVEKTFR (1)

WVPSTPSAAVPHSDKEDDVYNGMVISKRSM (0)

LKTPLSAKTTRRLHFNFGAGRGVCPGFHVSERNLFLAISRIL

*GFTINPTRDFGGAIVPINRDAVTPGLIVRPEDFESQASHHGSLVKGRKHLDSEGNFTPEF

IDSVFGKEK*

 

>CYP530A8 Mgr038 Mycosphaerella graminicola

66% to CYP530A3   Aspergillus nidulans

probable assembly error in the yellow region (or a pseudogene)

green from JGI model restores EXXR motif, cyan may be intron seq

MERPSIAVLVALVVFLYGFVRLLSVGRRPKDYPPGPPTIPVLGNIHQMPTRDAHLQFEKWAREYGDIYSLMLGTQTMIVL

SSDEAVKELLDRNSGNFSNRLEMYVGQVLMDIHPNGEVYASAFLLAVRGSATNAMQFRKMIHHLLNATVAPQYVPYQILE

NKQMLNDLLDTPNDFLKHIRRYSNALTTSMVFGWRTPTYEDDAVKQLFDGLSEFADINQTGVAALLDAFPLLRKLPETLL

PVQKKAKELHKVERELYLRHWLRAKKEIKEDTIKHCVGMAEAQKKEGFSDAQAAYISGTLLEAGSDTTSSTLYAFVQAML

XXXXXXXXXXXXXXXXXX

LSLKFSGMLSSSLIKSSTNDPDLG

AELPYIRMIMKETLR

WAPTTVLGAVSHVTTEAATYKGYYIPAGAGVMNNVWAIHNDPARSPNPHLFDPNRF

KDDKLSLYDSTSNPDPSKRDCFTFGAGRRICPGMHVAERSLFLGMARLLWAFSIKPAKDADGNEIVPQGQKFTQGFVCMP

EEFPADIVRTEWQVAGSQSLDHDTKQWIASPI

 

>CYP530A9 Uncinocarpus reesii

78% to CYP530A3   Aspergillus nidulans, 79% to 530A5

UREG_06613.1

MPKRDAHLQFAKWAKEYGPIYSLMLGTQCMIVLSSDEAVKALLDKKSGIYSDRQEMYIGQRVCSGGLRLLMM (0)

EYGPRWRG (0)

FRKMVHGLLNVTTSKSYVVYQVLENKQMLYEFLTEPDRFLYHIRRYS

NALTTTMVFGWRTPTYEDQKMKQLFDGFSEFAELNQAGAAA

LIDFFPWLRKLPDFLLPVHKLAKELHKHERALYLGHWLKAKEDIRKGTIKPCFCVGMAEAQKYDGFDDAQAAYISGTLLE

AGSDTTSSTLYAFVQAMLLYPDIQRRAQQEIDKAVGPDRLPDMNDQESLQYIRAIMKETLRWMPTTILGAVPHAVTQDDH

YMGYLIPKGAGVMNNVWAIHHDEKRHPNPRVFDPDRYINDRQSLGESAANPDGTKRDQFTFGAGRRICPGIHVAERSLFL

GMSRILWAFDIAPAPDAQGRPIMPDPDRLTQGFVCMPEEFPAKITPRSKERADIVTREWKAAEKTCLDPETKQWLLHPIDS

 

>CYP530A10 Coccidioides immitis

88% to CYP530A9 Uncinocarpus reesii

CIMG_04939.2

MLRLSVLLGLTAAAIAVARLLSIGRRPKNYPPGPPTLPLLGNIHQMPKRDAHLQFAKWAKEYGPVYSLILGTQCLIVLSS

DEAVKALLDKKSGIYSHRQEMYIGQEVCSGGLRLLMM

GYGPKWRG (0)

FRKMVHGLLNVTTSKSYVVYQTLENKQMLYEFLTEPDRFLYHI

RRYSNALTTTMVFGWRTPTYEDAKMKQLFDGFSEFAEINQTGAAALIDFFPWLRNLPDFLLPLHKKAKDLHKHEKALYLG

HWLKAKDDIRRGTIKPCFCVGMAEAQKSEGFDDDQAAYISGTLLEAGSDTTSSTLYAFVQAMLLYPEIQRRAQKEIDEVV

GADRMPNMDDQDSLQYIRAIMKETLRWMPTTILGAVPHAVTQDDHYMGYLIPKGAGVMNNVWGIHHDEKRHPSPRVFNPE

RYINDHQSLGESAANPDGTKRDQFTFGAGRRICPGIHVAERSLFLGMSRILWAFNIEPASDSQGRPIIPDPDRLTQGFVC

MPEEFPAKITPRSKARADFVTREWKEAEKACLDPQSKQWLLHPLE

 

>CYP530A11 Fusarium oxysporum

87% to CYP530A2

FOXG_13473 revised

MLTVQLFWVALAAIFVRLLFTGRRPKNYPPGPPTLPILGNIHL

MPTKDAHLQFRKWADEYGPVYSLILGTKPFIVLSS AQAVKDLLDKKSALYSDR

QEMYVGQILGSGGLRLLMM

GYGPTWRSFRKLVHSLLNVTTARSYVPYQDLENKQMLYEMIVQPDQFLQSIRRYSNALTTTMVFGWRSPIYRDPKLMQLFDG

FAEFAEINQTGIAALLDSFPVLRKLPDFLLPVQRKAKELHKKEKDLYMSHWLKAKQDIADGSIKPCFCVGLAEAQEKEKF

DDAQAAYISGTLLEAGSDTTSSTLYAFVQAMLLYPEIQRKAQDEIDKVVGKRMPTMDDEQSLQYVRACMKETLRWMPTTI

LGAVPHAVTQDDTYNGYLIPKGAGVLNNVWGIHMDPERHPNPRQFNPDRYRDDFQSLADAAANPDASKRDQFTFGAGRRI

CPGIHVAERSLFLGISRILWAFNIKPTVDKNGKPVLPDPDKLTQGFVCMPEEFPARIIPRSEEKKAQVIESWKKAEKDVL

DPETKQWR*

 

>CYP530A11 Fusarium verticillioides

96% to CYP530A11 Fusarium oxysporum = ortholog

FVEG_13927

MMLTVQLFWVAVAAIFVRLLFTGRRPKNYPPGPPTLPILGNIHL (0)

MPTKDAHLQFRKWADEYGPVYSLILGTKPFIVLSS AQAVKDLLDKKSALYSDRQEMYVGQILGS

GGLRLLMMGY

GPTWRSFRKLVHSLLNVTTAKSYVPYQDLENKQMLYEMIVQPDQFLHSIRRYSNAL

TTTMVFGWRSPIYRDPKLMQLFDGFAEFAEINQT

GI AALLDSFPVLRKLPDFLLPVQKKAKELHKKEKDLYLSHWLKAKQDIADGSIKPCFCVGLAE

AQEKEKFDDAQAAYISGTLLEAGSDTTSSTLYAFVQA

MLLYPEVQRKAQDEIDKVVGERMPNMEDEHSLQYVRACMKETLRWMPTTIL

GAVPHAVTQDDTYNGYLIPKGAGVLNNVWGIHMDPERHPNPRQFNPDRY

KDDFQSLADAAANPDAAKRDQFTFGAGRRICPGIHVAERSLFLGISRILW

AFNIKPTVNKNDKPVLPDPDKLTQGFVCMPEEFPARIIPRSEEKKALVIK

SWKAAEKDVLDPGTKQWR*

 

>CYP530A12 Fusarium verticillioides

53% to CYP530A3

FVEG_08430, not found in F. oxysporum genome

MSFSASVYLLIPVLILGLW

RLSTVGRRPAGYPPGPPTLPIIGNLHQIPNRKRHIQFQKWAEEYGPIYSLILGRKVMIVL

NSDQTVKDLVDKRGGIYSSRPESYIGQDVLSGGYRILFMVY (0)

VWKMARKLVHRILGVTAARSYIPYQDLESKAML (2)

NAVRFLKSSDDFVDHLRRYTASLTTQITFGSRTTSIQDERFKEAFD (0)

IFDRSSEMIGSR (0)

TAALLDLVPALRRIPDFLMPIKKEGRKIHYRELSLFRGFYLAAKKGLE

GGSAKPCVCVDLVKLQKEEGFSDTFASYLSGSLLQAGSETTASILIG

FVQAMVIFPDVAKTARGELDRVCGDRLPNLDDTPDLPYIHACMKESLR

WMPGFMLGIPHATTQHDSYLGYHIPKGATVIINVWAIHNDPKRHPSPRQFDP

MRYINDQQTSIEAANNPDPTKRDHYVFGAGRRRCQGMHIADRSLFLAI

SRLLWAFNFDRDVSAETGKAIIPDAEDVIEGIMSIPSPFPVRIVPRSTKRAE

AVAAAWSQAVDLLDEHGQWKVVPEDLLWTIPES*

 

>CYP530-un1 pseudogene Nectria haematococca

gw1.271.3.1

Necha1/scaffold_271:2439-4371

35% to 5047A1

39% to 530A3, 37% to 5056A1

MSISSASFLTLALPLIVAIALLEYGRKAIRSKSLPPGPSTSLFL (1)

GNLHQVPSSKAYLQ (2)

FQKWAKTYGPILGLKLGPQNLIILNSASLVRD (2)

LFDKRGAIYSSRTPNYIGSEIITRDNAHLLLMPYGPEWRNQRKAFQSILNITAVVSLQ

GLQEAEAVLTMSQLLQSPEQYYDHIRRYSTAVILSSVYGIRGPEFNHPNIQRLYHVQDQFTAILETGATP

PVDTFPFLKHLPTFLASWRRWALSIRREQRQLYFELLDNAKSRIQRGVHRGCFMDGLLSEDKRAKYSLDD

EHIAYVGGVLMEGGSDTTASTLLSFLMAMMKYPHVLRKAQKEVDKVCGLQRSLNFEDVGKLTYIR*CMTE (0)

TLRWRPVAAGGIPHV LIQ (1)

DDTY*GY*FPKGSIFIVNAW  (2)

AIHYDPDIYDRPEEFRPERYEGNEFGFKEHP*DASSLRKTYAFGAGRRVCPGQHLAENSL (0)

VINMAKLVWTFNMLPGEDPTTGKQLSVSEIDDSMATAWTNGFLTAPKKFPLLLA

VRSREHERVIKRECLEA*

 

>CYP530-un1 Aspergillus terreus

64% to CYP530-un1

ATEG_03951.1

MANVASTLILFSLFCCWVGWKIIYIARGGHRSPSLPPGPPTLPFI

GNLHQIPAQKAFLK (2)

FQEWSR*YGPIISLKTGPANLVILNKAKDVR (2)

LFDKRGAIYSSRLPNHIGNELITRDNVHLLL

MPYGQAWRNQRKIYQAILSITAVRSLTPLQEAEATLTLYQLSQTPELYYDHIRRYSTAVILSSVFGIRGPEFDHRNITRL

YHAQDQFTGILETGATPPVDIFPFLKRLPDFMSPWRRWARQIRAEQRQLYFELLQEVKSQRARGIYRNCFMSQLLDEKFA

EKYELDEEHIAYIGGVLMEGGSDTTASTLLSFLLAMTKYPKVFQKAQEEVDRVCGMSSSPTFDLIAKLPYVKHCVSE (0)

VLRWRPVAAGGLPHVLIQ (1)

NDEYKGFNFPTGTTFLANTWAIHHDPELYDRPDDFIPERYEHHPLGFKTDIKKTTDGIRKTYGFGAGRRICPGSHLAENSL

DINIAKIIWAFNIRAGTDPTTGRHLQDEEVNVDVATQWTDGFLIAPKPFPIVMELRSEKHREVF

RRELEDARKVFQSYED

 

>CYP531A1 N. crassa AABX01000205.1 cont3.366 NCU06327.1 (version3)

138867 MDSHAGLDGVLQPQVLKLTLRTLPSPFISFVVIPILSILGWYI

       ISWATNPLKKYPGPFLA (1) 138688

138607 GFTNWWRLYQTRTGRYHEIVYDLHKKYGPIVRIGPNTLDLDYP

       ELIKTIYSTDGKWLK (0) 138434

138374 TEFYHNNSAIVNGKITYHIFSTTSPTEHAKMKRPVAKYYSTSY

       VQALEPHVDVVLNDFCKYVEERFINVPGGPKELDFGEWLGY (1) 138123

138042 HAWDLISSVTFSRRFGYMEHGSDFDGTIEKAEAALRYFQTVGQ

       IPILDYFLDKNPIKRVGPPNLVHPARIAVQSFVARLQGKDENY

       DPKNPDYLQHFIDSKESHPDLVDDNQIISDVLVNLLAGADTTA

       IALRAVFYYMLKNRSVYNRVSEEIQAAGFDRSKPVPYSGARQL

       PYLEACVREALRIHPAAAMLLERYVPADGITLPDGSFVPAGTA

       VGLNPWVVSRNKSIFGEDSDTFRPERWLQQPGEDDEAFHVRMQ

       KWNAADLTFGNGSRICTGRNFAMFELYKVCATLLHRFEIELAD

       PTKEWKVWGSWFTIQKDVIAKMKVRG* 137062

 

>CYP531A2 Nectria haematococca

fgenesh1_pg.scaffold_15000295

Necha1/scaffold_15:796534-798330

60% to CYP531A1 N. crassa, 49% to 531C1

MSSIDTSYIEALVQGLDWKSLATAIVVVPILSITAWWIVSYFTSPLRKFPGPTLA (1)

GWTNLWRLSVVRKGSYHVKIKELHNKYGPVVRIGPNTLDFDFPELIKTLYGTDGKWKK (0)

TEFYHNNSAVVNGKITYHLFSTTDQAE

HARMKRPVVKYYSQSSVIGLESLFDSLIGDFCGHLEKRFMNGTEPTECDLGEWIGF (1)

YSWDANGAASFSQRFGYMDKGYDYDGTISIADKALDY (2)

FAAVGQMPFLDFLLDKNPVMRIGPPNLGNITRISLEHLISRLQGKDTNYNPKVPDFLQHFIESKETHPDL

VNDGIIMGYLLVNLLAGADTTAITIRAIFWYALHNPRAYKKLEEEIVAADLADVASFSSARALP (1)

YLEACAREAMRMHPGVCMLLERYVPETGLKLPDGSFVPPGTAVGINPYVAGRN

KTIWGEDADTYRPERWLQGEGETDAAYKERLRLYNAADLTFGGGSRVCIGRNLAQLELYKIVATLIKKYE

ISLVDPDQPWVVTGSWFPRQKGLKCYLKKRE*

 

>CYP531A3P Fusarium oxysporum

51% to CYP531A2   Nectria haematococca

FOXG_13597

MITQSSPTELSYVGTTVVLFAVLLWWIISTLTSPLRPYPGPLFA (1)

KWTNLWRLYQVRTQKYQWTIQDLHKKYGPIIRIGPN

LLDLDYPELIKIIYGTDHRWLK (0)

FYLNNSSMVDGKIKHNLFSTTSPAEHAQMKKPMAKYYSSSNVLTMEVKMNQVIKELCK

QIDNRFLEDNKSFDLAELLGL (1)

YTWDMIGSLTFSQPFGYMKKGHDFDGTIIASALSVDYFAQIGQMPFLDWLLNKNPMVRI

GPPDMSHVTHFSIAQLQRRLEQQEQRKSSEEPDFLDHFIDGMKSNPDSVDAKLVLNFLLANVLAGADTTVIALRAIFDFL

LQNQHAMTKLKSEILTLGFNDAEAVPYSRARSLPYLDAVIRESLRMHPSVAMPLERYVPNTGLTLPDGSFIPPGVAVGLN

PYIIGRNEVVWGEDTH*FRPDRWIKAEDESEGEYQRRLRKMNGADLT

FGGGSRICIGRHIALIQIYKAVATLVSRYDIQLADQ

NVRMKIISGWFPLQTGLFVRMRKRSEMGKL*

 

>CYP531A3 Fusarium verticillioides

80% to CYP531A3P Fusarium oxysporum, probable ortholog to the pseudogene

FVEG_11028

MDNLSIFTAIALCAALIWWVISTLMSPLRQYPGPFLAKWTNVWRLYQVRSQKYQWTIQELHKKYGPV

VRIGPNLLDLDYPELIKTIYGTDNRWLKFYLNNSTIVGGKISHNIFGTTSPAEHTQMKKPMAKYYSS

SNVLTLESKMNQVIEELCKQIDDRFLKGTESFDLAEWFSLYTWDIIGFLTFSQSFGYMIKGHDFDET

IMASALSVEYFAQIGQVPFMDRLLNKNPIVRIGPPDMSHITHFAITQLQRRLEQRDQDIHLEEPDF

LDHFIDGMNRNPDSVDASLVLNFLLGNILAGADTTSIALRAIFDFLLQNPHAMAKLKAEILAEGFDD

DAVVPYSTARSLPYLDAVVRESLRMHPSVAMPLERYVPDTGLTLPDGSFVPPGVSVGLSPYIIGRNE

RVWGEDANEFRPERWLKTEDEDDEDYQRRLRKMNSADLTFGGGSRICIGRHIALIQIYKVVATLVSR

YEIQLADLNVRMKIISGWFPRQTGLFVKMHKR*

 

>CYP531A4 Fusarium verticillioides

83% to CYP531A2, 49% to CYP531A3P

FVEG_14002 revised, no ortholog in F. oxysporum

MPSIHSTSYLHTLVQRSDWKRISAAFVFVPVLSIATWWIVTYFTSPLRKYPGPRLA (1)

GWTNLWRLWTVRKGSYHIRIKELHDQYGPVVRIGPNTLDLDIPELINTLYGTDGKWKK (0)

TEFYHNNSAVINGKITYHLFSTTDQAEHARMKRPIVKYYSQSRVIGLEPL

FDGLISEFCDHLEQRFMSGPEPTGFDFGEWIGFYSWDANGAASFSQRFG

YMDKGYDYDNTISIADKALDYFAAVGQMPFLDFLLDKNPIMRIGPPNLANI

TRISLEHLISRLQGKDENFDPKVPDFLNHFIESKKTNPDLVNDGIIMGY

LLVNLLAGADTTAITIRAFFWYTLHSPSVYSKLEKEILAAGIDNVASFSS

ARALPYLEACVREAMRMHPGVCMLLERYVPESGLKLPDGSFVPQGTAVGI

NPYVAGRNKGVYGDDADSYRPERWLQNEGETEEEYNKRIRLYNAADLT

FGSGSRVCIGRHLAQLELYKIIATLINKYEISLVDPAAPWVVTGSWFPRQRGLDCYLKKRA*

 

>CYP531A5 Fusarium verticillioides

62% to CYP531A2 Nectria haematococca

62% to CYP531A4 Fusarium verticillioides

57% to CYP531A3P Fusarium oxysporum

FVEG_06505, no ortholog in F. oxysporum

MTLNVILLNYSLTTLLFGGITALLAAAIIWLVFSELTSPLRSYPGPF

LARWTNLWRLYQVQTQKYQWTIKDLHEKYGPVVRIGPNLLDLDHPELIKTIYS

TDGKWCKTEFYHNSSAVINGKITYHLFSTTDQAAHARLKRPIVKYYSM

SNILNLESHVDTVISDFCDHLETRFMDKQSQEKQFDFGQWIAF (1)

YTWDMISSATFSQRFGYMEKAYDFDGTIH

TADQVTDYFAMVGQMPFLDYLLDKNPIKRIGPPNLGNVTRISVEKMMSRVDK

LDEIDRKPAEELDFLDHFLNAMKKSPDLVDTSVITGYLQVNMLAGADT

TAITLRAIFNFLLENPRTMTRLKEELLSLDYDGTKEKIVSYKSARAVPY

DAVIRESMRMHPGVGMLLERYVPDTGLQLPDGSFVPGGTAVGLNPYVIGR

NKGIWGDDADEFRPERWLPKTGEGDEAFQQRLRQMNGADLTFGGGSRICIG

RNLALLEIYKVVATLVSRYEILSVPGQKMKIISTWFPRQSGLICQLRKR

 

>CYP531B1 old name = CYP569A1 Magnaporthe grisea MG07856.4  44% to CYP531A1

AACU01000959.1 cont2.1455 PROBABLY SHOULD BE CYP531B1

MSAAAVATWAFVLLATCAAWNVLSCWRSPLRRYPGPFLARWTNLWRFYHT

LRGDIHLVNLRAHQRYGPVVRTGPNQLDLDLPALVKTIYSSDGRFRKTDF

YPPASTVVDGRVAYNLFSLRDPAEHARQKRPVAKHYSLAGTLALQPHVDD

TIRLLRRSLDERYVGADGFGEGFDLGEWVKMYAWDVIGQVTFSKHFGYLS

SGRDFDGHLWMSDRGADYLSSVSQIPWLDRIVDKNPIFPVGGATALLGPT

MKRLDDRYAGRDDRDPVQAAAQPDFLDRFLEVQRAQPDVVDRQRVLSYLS

INMLAGADTTAITIKAVLYYALRTDGVWGRLVTACEDAGLAAGEIASFAQ

ARAVPYLEAVVREAMRLHPGVAMVLPRCVPDGGLELPDGNVVPPGTSVGM

NPFVLGRNRTVWGPDADDFRPERWLRADGETEEAFAVRLAAMNAADLTFG

AGSRVCLGKHLGLLQAYKVVATLAVSYDLEMLEPEKEWWVRSAFFMRQGE

LKVSMRRRD*

 

>CYP531C1 Fusarium graminearum FG02982.1 AACM01000145 FGcontig1.145_scaffold2

MSKHTLYETSILGPARNIFSLVSPSVFLLLVVPASTLSLWTLASYFTSPL

KKYPGPFLAKFTRLWYMYQASTGDSHLVLERLHKRYGPIVRITPDIIDVD

IPEIINTIFSTKDDWLKTPFYHGSSALVNGHIVLNTFSQTDPVKHKKGRQ

PIAKLYSSAGVSTLEPHMNKVINQLCDELEKRFTGHNAGQVCSLGQWILF

YAWDVVGAITFSQPIGYLKKGCDFDGTLKNADKAMDYFTVVGTMPFLDRI

FDKNPVFHMGPPGFNTSTEISVKHLIDRYQGNDKENHDPAHPDFLDKFIE

IKNSKPDEADDAQIISWLMVNMIAGADTTAITIRSVLYFSLKHPRVWKRL

TEEILRAGFQQVPAYKDVKALPYTDAVCREALRMLPGVAMTMERFVPKEG

FVLPNGDFLPGGTIVGMNPYIVARNKSVYGDDADDFRPERWMRSEDETEE

QYQIRLLAMNQADLSFGGGSRICIGKYIGLFQTYKVIAILLTRFEIELAD

PNKEWKVTNSWFPRQEGLEARIRKRTGSRLPKSSY*

 

>CYP531C2 Nectria haematococca

e_gw1.27.133.1

Necha1/scaffold_27:386590-388379

76% to 531C1

MNHTSYLDTSILESAEGILRSNSSLFLYLIVPVSTISLWTIGSYFSSPLKKYPGPFLA (1)

KFTRLWYLYQAW

TGDSHLVLDKLHKQYGPIVRITPSIIDVDIPELIKTMFSIKGDWLK (0)

TPFYHGSSALVNGQIVYNLFSQTD

PVQHRKERQPIAKHYSSAGITTVEPHMDKVIDQFCNELEKRYIAGDNAGKVCDIGQWTLF (1)

YTWDVVGAVTFSQPIGYLEKGYDFDGTLSNADKAMDYFTVVGTMPFLDRVFDKNPVCHIGPPGFNTITGISIKHLVDRYQ

GNDKDYHDPEKPDFLDKFIEAKNADPENVNDPQIVSWLMVNMIAGADTTAITIRSSLYFSLKNPSVWNRL

TDEVLAAGFYGRTPPSYKEVKALPYVDAVIREALRMLPGVSMTMERYVPREGFVLPNGDYLPEGTIVGMN

PYILARNKSVYGEDADTFRPERWLRDEANGETEEQFQKRLLLMNQTDLSFGGGSRICIGKNMGLFQTYKV

LATLITLFEIELADPKKEWKVINSWFPRQEGLEVKLKKRAL*

 

>CYP531C3  Aspergillus oryzae

          GenEMBL BAE65935.1

          58% to 531C2

MDSFLFLALDYSLRGFAVSLFGTLWIALVFWYLATYLISPLRKIPGPFLAGWTNLWRMYHVTQGQSQVVL

HELHQKYGPVVRIAPNVVDLDLPEMIKTIYNTKGDYRKTEFYHGSSAKSNGRIIYNLFSECDPDIHAQQK

RPIAKYYSLTGVLPLEPHIDEVINYLCQRLEEEFINGLNAGITCKLDQWLLFYTWDVVGQATFSEPIGYL

KNGFDFDGTIAISDTAMDYFSLVGQLPVLDHLLDKNPIYRIGPPAFGNITNISITHLLDRLQGKDTSYHD

ANKPDFLDRFIDAKDKYPDIVDDSQIISYLMINMIAGADTTAITLNAAIYFALKDRRVWARLQKEIRACQ

SSLDAAPSAVPYNIASALPYLNAVVREAMRMHPGVAMTLERYVPPGGLTLPNGQYIPQGSIVGMNPYVIA

RNRSVWGEDSDVFRPERWLRDDSQESEEEFQARLRLMNNSDLAFGAGSRICIGRNLGLLEVYKVMATLIS

RYDIELAHPHRDWKTHNSFFVRQEGINVKLSRRS

 

>CYP531C3 Aspergillus flavus NRRL3357

AAIH02000016.1

98% to CYP531C3 = ortholog

AFL2G_11285

40898  MDSFLFLALDYSLRGFAVSLFGTLWIALVFWYLATYLISPLRKIPGPFLA ()

40659  GWTNLWRMYHVTQGQSQVVLHELHQKYGPVVRIAPNVVDLDLPEMIKTIYNTKGDYRK  40486

40485  TEFYHGSSAKSYGRIIYNLFSECDPDIHAQQKRPIAKYYSLTGVLPLEPHIDEVINYLCR  40306

40305  RLEEEFIDGSNAGITCKLDQWLLF (1)

       YTWDVVGQATFSEPIGYL  40126

40125  KNGFDFDGTIAISDTAMDYFSLVGQLPVLDHLLDKNPIYRIGPPAFGNITNISITHLLDR  39946

39945  LQGKDTSYHDANKPDFLDRFIDAKDKYPDIVDDSQIISYLMINMIAGADTTAITLNAAIY  39766

39765  FALKDPLVWARLQKEIRACQSSLDAAPSAVPYNIASALPYLNAVVREAMRMHPGVAMTLE  39586

39585  RYVPPGGLTLPNGQYIPQGSIVGMNPYVIARNRSVWGEDSDVFRPERWLRDDSQESEEEF  39406

39405  QARLRLMNNSDLAFGAGSRICIGRNLGLLEVYKVMATLISRYDIELAHPHRDWKTHNSFF  39226

39225  VRQEGINVKLSRRS*  39181

 

>CYP531C4 Aspergillus fumigatus A1163

ABDB01000058.1

94% to CYP531C4 Neosartorya fischeri

50937  MDAFQSVAFDFGRRGIVNPFVGALWAALIVWYLLTYLISPLRRFPGPILA (1)  51086

51149  GWTNLWRMYHVRKGKVHILTTELHKKYGPVVRIAPNVVDLDMPEMIRTIYSTRGDYRKTE  51328

51329  FYHGSSAKSNGKIIYNLFSECDPQVHALQKRPIAKYYSMSGVLPLEPHIDETIGFLCRRL  51508

51509  EEEFIDGPKAGRPCDLGQWLLY (1)

51633  YTWDVVGKVTFSEPIGYLEKGYDFDHTIAIADKAMDYFALVSQLPILDHLLDKNPIYRIGP  51815

51816  PSFGTVTNISIQHLVDRLQGRDTAYHDPSKPDFLDKFIEAKATFPHVVDDAQIISYLMIN  51995

51996  MIAGADTTAITLNAALYFALKNPAVWKRLQEETASLHCEGSPIVSFKTAQDLPYLNAVIR  52175

52176  EAMRMHPGVAMCLERYVPDEGLTLPGGQFIPGGCIVGMNPYVLARNQSVWGEDADVFRPE  52355

2356  RWLRDPTRETEEAYQERLKRMNAADLTFGAGSRVCIGRNLGMMEVYKVVATLVSRYEIEL  52535

52536  VDPKGEWKTHNSFFVRQEGIEVRLRRRST*  52625

 

>CYP531C4 Neosartorya fischeri

72% to CYP531C3

NFIA_055060

94% to CYP531C4 Aspergillus fumigatus

MDAFQSVAFDYGRRGLVNPFFGALWAALIVWYLGTYLISPLRRFPGPILAGWTNLWRIYHVRKGKVHIVTTELHKKYGPV

VRIAPNVVDLDMPELIRTIYSTRGDYRKTEFYHGSSAKNNGKIIYNLFSECDPQVHAQQKRPIAKHYSMSGVLPLEPHVD

ETIGFLCRRLEEEFIDGPKAGRLCDIGQWLLYYTWDVVGKVTFSEPIGYLEKGYDFDHTIAISDKAMDYFALVSQLPILD

HLLDKNPVYRIGPPSFGTITNISIQHLVDRLQGKDTAYHDPSKPDFLDKFIEAKATFPNVVDDAQIISYLMINMIAGADT

TAITLNAALYFALKNPAVWKRLQEETSSLHSEGSTIVPFKTAQDLPYLNAVIREAMRMHPGVAMCLERYVPDEGLTLPDG

QFIPGGCIVGMNPYVLARNQSVWGEDADFFRPERWLRDPTRETEEAYQERLRRMNAADLTFGAGSRVCIGRNLGMMEVYK

VVATLVSRYEVELADSKGEWKTHNSFFVRQEGIEVRLRRRSI*

 

>CYP531C5 Aspergillus terreus

65% to CYP531C4 Neosartorya fischeri

ATEG_06454.1

MTQQIYMDSIMNWCFHMGSLIAWACVFAVPIWYLTAYLISPLRQFPGPVLAGWTNLWRMYHVRKGNIHVVVHNLHKKYGP

VVRIAPGVLDLDLPDLVRTIYNAKGDFRKTEFYHVSSTKTNGKIIYNLFSERSPEQHLRQKRPIAKYYAMTEVLTLEPHV

NEMIQCLCQRLEEEFVAKPKSGSVCDLSEWMRYYTWDVVGKATFSNPFGYLQKGCDFDNTISIADQAMDYFALVAQLPVL

DYVLDKNPVYRIGPPSFGNITAISIQQLMSRLQRREAGTSQGASQDFLDRFIDAAAHDSDTIDHGQIISWLMINMIAGAD

TTASTLNAVLYYSLKHPAVWARLQQEIAMQEVEQDATACVLPFKSAHDVSYLEAVIREAMRMLPSVAMTLERYVPEGGLV

LPDGRRIPGGCAVGMNPYILARNSSVWGECPDEFRPERWLRDTAHESEAEYQERLQRMNNTDLVFGGGSRVCLGKNFALV

QIYKVLATLVSRYDIELASPDREWSTYNSFFIRQSGLYVRMSRRSK*

 

>CYP531D1 Fusarium graminearum FG09351.1 AACM01000383 FGcontig1.383_scaffold6

MALFTLSLGYLMYAGVAFVIARVLYEFFLSPLRHIPGPGPSKYTDFYRAY

LTTKGHVDSHTRAWHRKWGAAVRVGPNTVSISDPDLIKVIYTTRNPWRKT

NMYRPNDVLVNGQRIQNIFNTQDEDFHLKYTKPIRGFWTLPKMLEAEPLM

DETLCELITHLDNRFASTGDVCKMDDWVSYYAWDAAANISFGRTYGFMDK

GGDVEDIIAESTAGLKYFAPVSQIPWLDEWLDKNPIHRVGPRPLVNGVIY

TIKILTEYQQQLASGAAKRKPVDLFIDKYNSLKETVDYVDDQQVINWLML

NVLAGGDSTAGAMRAPAYHLLKNPSVCEKMVAELRSANLTLPVPQWKEIS

QLPYFDAVMRESMRISPAVGLILEREVPKEGLELPDGRFIPSGAKIGINP

CVVTRDVGVFGEDVDVFRPERWLRGKGETEEGHASRVRRMHECTELMFGH

GSRVCMGKYMSKLEMYKMFATLYVNFDVRITDPSHTWKYENNWFMYHRDI

PCKISRREKA*

 

>CYP531D2 Aspergillus nidulans AN5665.1 52% to 531D1 53 clan looks intact

MTGVLLAGANASLWMQIPVVFAVLLLSKLIWDAFFSSLRVFDGPLPAKFT

DIWRAWQAFDGHIDRTYTKLHRKYGSVMRIGPNALSISDPSMIRVIYSTR

NPWKKSEAYRPNDVVLNGQRVSNLFNTQDEKWHNQNILPIRHLWQMTKVL

EYEPLIDKTLNKFVDKLAARFGGGEVCPADEWIGYFAWDVTANFSFGRHY

GFIDQEKDVDNLITDSTKGLIYFAPVSQIPWIDHLLDKNPIVRIGPKPTL

TGVLYAFKVVAEYQAQLNSNKVKPGNVDHTLDKYVQLKKTHPDVVNDVQI

VNWLMLSILAGGDTSSATMRATVYYLAKNADAYKKLVAELTTANLTMPAQ

WKDIRELPYLDAVIRESMRINPGIAMNFERVAPEGGYTLPDGRYIPAGTK

VGINPAVTNRDYAIFGEDSDSFRPDRWLKRDGESDEEYQERHKRMHDTCD

FVFGAGARVCMGRYLAMLEIKKLIATLYSTFDLHLVDPKHEWTYRNAWFV

YQQNMPMIITRRKLSA*

 

>CYP531D3 Nectria haematococca

e_gw1.20.308.1

Necha1/scaffold_20:255173-256867

76% to 531D1, 50% to e_gw1.3.1764.1

MALLTISFTYVLYGGVALIVARVLYEFLLSPLKHIPGPILAKFTDFYRAGLTTQGHVDSTTRGWHKKWGS

AVRVGPNAISISDPDLIRVIYTTKNPWRK (0)

SNMYRPNDVVINGQRIQNIFNTADKNFHAKYTKPIGGFWTL

TKILDLEPVMDETLGELVGHLDRRFANTGDVCKMDDWVAYYAWDAAANISFGRHYSFMEEGGDVGGIIAE

STAGLKYFAPVSQIPWLDEWLDKNPVWRIGPRPLVNGFVYTVKILTEYQQQVASGAAKRKPVDLFIDKYN

GLKDTHDFVDDNQVINWLMLNVLAGGDSTAGAMRSVAYHVVKDPAVYKKLIAEIESANLSLPVPQWKEIS

QLTYFDAVMRESMRIAPAVGLMLEREVPEGGFELPDGRFIPANTKVGINPCVVTRDTGVFGDDVDTFRPE

RWLRKDDETEDEYAHRFRRMHECTDLMFGHGTRVCMGKHMAKIEMYKLFATLYANYD (0)

VRLTSPDHEWKYHNSWFMYHSDIPCILTPSQRT*

 

>CYP531D4 Nectria haematococca

e_gw1.3.1764.1

Necha1/scaffold_3:3276976-3278701

53% to 531D2, 38% to fgenesh1_pg.scaffold_15000295

MAFTQLEYAHLLGIGAVLLCLAVVWQLLLSPLRAFPGPFVARFTDLWRAAAVLPGQIDNVNRRWHKRYGS

AVRVGPNTIILNGPEMIKIVYSTKKAWVK (0)

SDMYMINDVLLNGKRLANIFNTRDLEWHAQQIKPISKFFSM

SRLMDIEPLLDVTINMLTSKLNEKFVDGPSKGKVCMMDDWLGW (1)

LAWDSMANVTFGRHYGFIEKEQDVDGLIKDSVNSMRYFAP (0)

VCQIPWLDYLLDKNPIKRIGPKPQLTGTMYAVRAISEYKQEIAEKGLKSQNVPHLLD

KYLGLEEQYPGMVNDAQLVNWLLPTVIAGGDTTASAMRAVVYFLAKNPETKQKLFLELDQANLETPAQWK

DLKELTYLDAVIREAMRVCPGIGLAPERVVPEGGHILPDGRFLPAGTKVGLNPAVTSRDTGVFGADSDSF

VPERWLRQDGEAEDAFNQRFRRMHEVLDFVFGGGTRVCLGKNLAKLEIYKAIATLYSLYD (0)

INLVDPRHEWKHYSCWFVYQLDIPTAISRRT*

 

>CYP531D5 Fusarium oxysporum

86% to CYP531D1 Fusarium graminearum

FOXG_05863

MALSSFDLSYVLYVGVAFVVARIFYDLLFSPLRRIPGPLAAKYTDFYRAFLATKGNVDGHMREWHQKWGSAVRVGPNTIS

ISDPDLIRVIYTTRNPWRKTNMYRPNDVVVNGQRMQNIFNTADEDFHTKYTKPIRGFWTLPKMLEAEPIMDETLCELITH

LDNRFASTGYVCKMDEWVAYYAWDAAANISFGRNYGFMDQAADVENIIAESTAGLKYFTPVSQIPWLDEWLDKNPIWRIG

PRPLVNGVLYTVKILTEYQQQVASGAAKRKPVDLFIDKYNSLKETVDYVDDQQILNWLMLNVLAGGDSTAGAMRAPAYHL

LKNPSVCEKMVAELRSANLTLPVPQWKEISQLPYFDAVMRESMRISPAVGLILEREVPKEGLELSDGRFIPAGTKVGINP

CVITRDVGVFGEDVDTFRPERWLRSKGESEQGHAHRVRRMHECTEMMFGHGSRVCMGKHMSKLEMYKMFATLYVNFDVRI

TDPNHRWKYDNSWFMYHKDIPCTISRRQKS*

 

>CYP531D5 Fusarium verticillioides

98% to CYP531D5 Fusarium oxysporum = ortholog

FVEG_03740

MDLSSFDLSYVLYVGVAFVVARIFYDLLFSPLRRIPGPLAAKYTDFYRAYLATKGNV

DGHMRAWHRRWGSAVRVGPNTISISDPDLIRVIYTTRNPWRKT

NMYRPNDVVVNGQRMQNIFNTADEDFHTKYTKPIRGFWTLPKMLEAEPI

MDETLCELITHLDNRFASTGYVCKMDEWVAYYAWDAAANISFGRNYGFMDQ

AADVENIIAESTAGLKYFTPVSQIPWLDEWLDKNPIWRIGPRPLVNGVLYT

IKILTEYQQQVASGAAKRKPVDLFIDKYNSLKETVDYVDDQQILNWLML

NVLAGGDSTAGAMRAPAYHLLKNPSVCEKMVAELRSANLTLPVPQWKEIS

QLPYFDAVMRESMRISPAVGLILEREVPKEGLELSDGRFIPAGTKVGINP

CVITRDVGVFGEDVDTFRPERWLRSKGESEQGHAQRVRRMHECTEMMFGHG

SRVCMGKHMSKLEMYKMFATLYVNFDVRITDPNHRWKYDNSWFMYHKDI

PCTISRRQMS*

 

>CYP531D6 Aspergillus clavatus

72% to 531D2

ACLA_059870

MTVLTYLLPTSWWGFIPVVVGVFVLTRAVWDALLSPLSAFPGPLAAKVTDFWRAAQAYGGYIDRKYNVLHRKHGSVVRIG

PNALSIGDPNLIRVIYSTRNPWKKSDMYKPNDVLINGHRMSNLFNTQDEHWHNQNILPIRPLWGMGKVLEYESLIDETLN

LFTGKLRARFTGSNGRTICPIDEWLGYFAWDVTANISFGRHYGFIEQEKDVDNLITDSTKGLIYFAPVSQIPWVDRLLDK

NPIVRIGPKPTLTGVLYTFGVVTEYQTQLKEKKSRLNAVAHTLDKYVQLKDSYPDIVDDNQIVNWLMLSILAGGDTTSAT

MRAVVYFLMKTPAANRKLAAELDAAKLDIPAQWRDIRELPYLDAVIRESMRLNPGIAMNFERIVPAEGFTLPDGRFIPGG

TTVGINPAVTNRDPGVFGGDVDVFRPERWLRGAGEDQAQFQARHRRMKDTCDFVFGGGGRICMGRYLAMLEIYKLIATLY

SQFEIQLVDPNHEWKYRNAWFVYQSDMPVIIRPRQRD*

 

>CYP531E1  Aspergillus oryzae

          BAE63827.1

          45% to 531D2, 40% to 531D1, 39% to 531C1, 40% to 531A1

          44% to 531D4, 42% to 531D3, 38% to 531C2, 37% to 531B1

MLSITELIMDLGLAIVILGVSHLLWNYLSSPLKAFPGPLWASFTNLWRLQDVFKGRCDITHNQLHRKYGT

AVRMGPKVLSLSDPSVIPHVFNSKNPWMKSDMYNVNDVIVSGVRLKNLFSHQDEKWHSTYIRPVKGLYSM

TKVQDMEPGVDVTINLFMDKLRERFVEKGQLCDMADYLNFFAWDVMSQITFSQNLGILEAGSDYQGFLGR

SNKSLDYFASISQMPILDLLLDKNPIVRLGPPTFVWANIFSLEQLQKRLSGGTPPSGHTDFLDKFLETKK

KYPDLVNDNTVVTYLLSNTLAGSDTTGSAMCSAVYHILKHPRVHRKLREELYAAKVPLPAKWKDLQGLTY

LDAVMRESMRVNPGVGLMIERIVPEGGFTLPDGRFIPAGTIVGMNPWVINKNEAIFGANTEEFIPERWLP

SPGESDQAYQARFTKMKSTDFTFGAGPRMCMGRYLSQLESYKLIATLFSTFEMELPSLDHTWHVTNSWFV

RHKSIPVKMRERTDLAVSV

 

>CYP531E1  Aspergillus flavus

99% to CYP531E1  Aspergillus oryzae

AFL2G_10670

MLSITELIMDLGLAIVILGVSHLLWNYLSSPLKAFPGPLWASFTNLWRLQDVFKGRCDITHNQLHRKYGT

AVRMGPKVLSLSDPSVIPHVFNSKNPWMK SDMYNVNDVIVSGVRLKNLFSHQDEKWHSTYIRPVKGLYSM

TKVQDMEPGVDVTIKLFMDKLRERFVEKGQLCDMADYLNFF AWDVMSQITFSQNLGILEAGSDYQGFLGR

SNKSLDYFASISQMPILDLLLDKNPIVRLGPPTFVWANIFSLEQLQKRLSGGTPPSGHTDFLDKFLETKK

KYPDLVNDNTVVTYLLSNTLAGSDTTGSAMCSAVYHILKHPRVHRKLREELYAAKVPLPAKWKDLQGLTY

LDAVMRESMRVNPGVGLMIERIVPEGGFTLPDGRFIPAGTIVGMNPWVINKNEAIFGANTEEFIPERWLP

SPGESDQAYQARFTKMKSTDFTFGAGPRMCMGRYLSQLESYKLIATLFSTFEMELPSLDHTWHVTNSWFV

RHESIPVKMRERTDLAVSV

 

>CYP531E2  Aspergillus oryzae

          BAE59053.1

          52% TO 531E1, 40% to 531D2

          60% to 531E1 after making adjustments for gaps

          revised 3/19/2009

MDIAFKLVLLGATLILSHLIWNYFRSPLKSFPGPFSANLSNLWRLQDVFKGLCDITHNELHRRYRSAVCI

GPNVLSLSDPALINQVYSTRDPWVKVSSERTQDPQHSDVYNVNDSVVSGVRIKNLFSHQDEQWHANQELE

PGVDITINLFLE

SEYINY (1)

FTWDTMSQLSYSQSIGMLEAKNDRFGILEVSNRSLDYFAS

VCQIPMLDLLLDKNPICRLGPPSFGWSVKFSAEQYQERFTEGKQSHNGIKDFLDRYIETKSKMPNIVDDN

VAQMYLVLNIIAGSDTTARAISAAVYYEELRGANLSTPPQWKEIRSLPYLDAVMRETMLVHPGVGLLLERIV

PKGGFTLPDGRFVPEGTIVGMNPWVINRNRTVFGPEPDSFKPERWLPAEGEHDEAYQTRFSKMKGTDLTF

GAGPRACLGRYISQLESYKFVATLFTMFD (0)

LEHPDHEWEVTNSWFVRQKNIPV (0)

PSSSSSSLVVSL*

 

>CYP531E2  Aspergillus flavus

99% to CYP531E2  Aspergillus oryzae

AFL2G_04353

MDIAFKLVLLGATLILSHLIWNYFRSPLKSFPGPFSANLSNLWRLQDVFKGLCDITHNELHRRYRSAVCIGPNVLSLSDP

ALINQVYSTRDPWVKVSSERTQDPQH (0)

SDVYNVNDSVVSGVRIKNLFSHQDEQWHAN (2)

QELEPGVDITINLFLE

SEYINY (1)

FTWDTMSQLSYSQSIGMLEAKNDRFGILEVSNRSLDYFAS ()

VCQIPMLDLLLDKNPICRLGPPSFGWSVKFSAEQYQERFTEGKQSHNGIKDFLDRYIETKSKMPNIVDDN VAQMYLVLNIIAGSDTTARAISAAVYY

EELRGANLSTPPQWKE

IRSLPYLDAVMRETMLVHPGVGLLLERIVPKGGFTLPDGRFVPEGTIVGMNPWVINRNRTVFGPEPDSFKPERWLPAEGE

HDEAYQTRFSKMKGTDLTFGAGPRACLGRYISQLESYKFVATLFTMFD (0)

LEHPDHEWEVTNSWFVRQKNIPV (0)

PSSSSSSLVVSL*

 

>CYP531F1 XM_001227270.1 Chaetomium globosum CBS 148.51 revised C-term

53% to CYP531A2 add to nomen

MASASPAANGGIPPAEGFTLDLTTLLAGVWGNKLSALVLGPSVA                    VFLWFFVAYQTSPLKKYPGPFLAGWTNLWRVWQVISGEYAPRMKKLHEKYGPIVRIGP                    NLLDLDIPELYRVIYGTDGKWIKSDFYKNSSSIIDGKITYHMFSEVDPTLHALIKRPV                    VRHYSVPAVLVMEPLMDTIIKEFIDTLQKRYVEPKKTCEFGEWLSYYAWDFMGMVTFS                    TKFGYMEKGTDFDGTLAIADKSIDYLALCGQMPWTDYILDKNPIYPIGPPNIGNVTRI                    AVQNLTARLKGEDKNFTEGNPDFLQYFIESKSTHPELVNDG

(seq gap)

SRLQRDVRANFAAFEPA                    AHSKARALPYLEAVVKETLRYHPAVSMIMERIVPEGGLTLPDGSVVPAGQMVGMNPYI                    VGRNKEVFGEDAEEFNPDRWLRREGEGEEEHKERMARWGQSLLAFGGGYRICLGRNLS                    MMEVYKVVPTLLASFDIELEDPNEVWWTCSRWFYRTKGVICKLKPRSS*

 

>CYP531F2 DV545173.1 Chaetomium cupreum EST

86% to XM_001227270.1   Chaetomium globosum

LSMMEVYKVVPTLLAAFDVELVDPEEKWWTCSRWFYRTKGVICRLKPRST*

 

>CYP532A1 N. crassa AABX01000767.1 cont3.215 NCU04089.1 (version3)

33962 MITDLIIKVLAQHWPAILCLVLVGWLVRNRYHNGLNKYPGPFLA

      SLTDWWRFIDVYKRRPEVTHIALHKKHGPIVRLGPNTLSFSDPE

      AIKTIYGLNKGFVK (0) 33657

33584 SDFYVVQQSTVKGHNLPSLFSTTDNEFHMQFRRCVNSAFAMSAL

      VQYEPFVDNTTKLFLEQTEKLYVEGGEKACDFTRWLQFYAFDVI

      GEITYSKRHGFIEHNKDIDGIVSYLTKLFNYVAP (0) 33219

33153 IGQIPLLDRLFLKNPLYLKLSQWGLIDGTMPVAAFARARMAERL

      PELQTEKTAILPTSEKQAQLKIQTPDLLSKFLAARSARPNFMTD

      TLVQTMAVSMAFAGSETTAISLSAVFYYLLRTPRALARLREEID

      EAARQGKFSDYETGLVTWAEAQTLPYLDACVKEAFRLHPAPGLP

      MERVVPPQGMEIVPGQFVKGGTIVGVSAWVLHRDESIFGERVEE

      FRPERWFVDETVAKEDMEGKKREEEEKRIKTMNGHMLQFGMGSR

      TCIGKNISLLEIYKLVPTLLRRFE (0) 32290

32200 IEFDDPSREWEIVNAWFVKQNNFITRFKVRDIVMPENKEY (2) 32081

32012 KFDGRGVN* 31986

 

>CYP532A2 Magnaporthe grisea MG07795.4  65% to CYP532A1

AACU01000949 cont2.1444

MQGLTAPILHFLAANWLPVLVAVTAAWLARNRFQKGLHKYPGPFLASLTD

FWRVWDVYKQRPEVTHRKLHERYGDVVRLGPNALSFSDPKALKAIYGLNK

NMVKSDFYIVQQSVVKGRRLPSLFSTVDESFHAKFRRCVNAAFAMSALVQ

YEPFVDNTTKLFLDQTQRLFADKPAGDDSGCDFGRWLQFYAFDVIGEITY

SKRHGFVEKNEDIDGIVSYLSKLFLYVAPVGQMPLLDLLFLKNPVYLKLS

QWGLFDSTFPVAKFARARMAERLPAGGSMNGGFGAGADEKPAAEPHPSTK

SPDLLSKFLAAQEARPDFMDDSLVQTMAVSMAFAGSETTAISLSAVFYYL

LRNPSAMARLRREIDDAARRGEFEDGETGLVTWHESQRLPYLDACVKEAF

RVHPAAGLPLERIVPEGGMEIAGHFVPGGTIVGVSAWVIHNNKDIFGEDV

DVYRPERWLVDEELVKAAGPAGHEVRAREEQRIKEMGGMMMQFGMGSRTC

IGKNISLLEIYKLVPSLLRRFDIDFKDPQQEWELVNAWFVKQNNFWAKFK

TRELVQPDEKSGAIAR*

 

>CYP532A3 Fusarium graminearum FG01745.1 AACM01000092 FGcontig1.92_scaffold1

MHGGLIETLLSVLAQHWVLALTTVTFAWLVRNRFHHGLNKYPGPFLASIT

DWWRFFDVYGQKSEATLRKLHAKHGDIVRLGPNNLSFSDPSVLKQIYGLS

KGYVKSDFYIVQQSVVQGHRLASLFSTTDNDFHSQFRRSVNAAFSMSALV

QYEPFVDNTTKLFLDQTEKLFAKKSEVCDFTQWLQFYAFDVIGEITYSKR

HGFIEKNEDVEGIVAYLSKLFLYVAPIGQIPFLDLLFLKNPIYLKLSQWG

LFDSTFPVAKFARARMAERLIPELHASDGLPTSNIKKQPLGQDLLSKFIA

AREARPDFMTDTLVQTMAVSMAFAGSETTAISLSSVFYYLLRNPACYEKL

KKELDDAAKAGVFSDYETGLVTFTEAQKFPYLHACVQEAFRVHPAPGLPL

ERIVPPQGAEINGEFIKGGTVVGVSAWLIHHRQEIFGPDTDAYRPERWLP

DPKLNPEDEDKRIKKMTGMMFQFGMGSRTCIGKNISLLEIYKVVPSLLRR

FEISFDDPTKEWKTINAWFIKQTDFNVRFTKRELVQPEFSEK*

 

>CYP532A4 Aspergillus nidulans AN2125.1 56% to 532A3 top part

revised 7/18/07

MALVTEILDHTEPLSNYGLYILPFFILLYLAQQYFHNGLHRYHGPILAKF

TNLWRFLDVRGRRPELTHIALHRKYGDVVRLGPNTLSFASPSAIKVIYGL

NKGFTKANKSQSEFYPVQMTVSKGEPLPSLFSTLDESFHANLRRSVNHAF

SMSSLVQYEPMVNQTVELFLDQTAALFANSGKTCDFARWLQFFAFDVIGS

ITYSKRHGFIEKNEDIDGILKSLARIFDYAGPVGQMPWLDKWLWKNPIYD

VLQRWGIADNSHPVAIFARQRMNERLMADNGTRTEAEQQQDLLTKFIQAG

KDRKDFMTEKRVLTMAVSMAFAGSETTAISLAAVFYYLLKNPEYMARLRG

ELDNAVAHGIVENRSSGLVTWSESQKLPFLDACIKEAFRIHPAAGLSLER

VVPASGIDIAGHFIPGGTIVGCSAWVLHRREEIFGPDVDSFIPDRWLNVS

AERLKTMNGTMLQFGAGARTCIGKNISLMEIYKLVPSFLRRFDIQLAHAD

QEWELWNAWFVRQYNFKTKFTARNFEV

 

>CYP532A5 Nectria haematococca

fgenesh1_pg.scaffold_1001305 NEW

Necha1/scaffold_1:4090277-4092053

83% to 532A3

This gene model seems correct

MNNGLIETLLSNLARHWVLVLVSLVLGWLVKNRYHHGLNKYPGPFLASLTDWWRFVDVYGQRPEVTLQAL

HAKHGDIVRIGPNTLSFADPAVLKSIYGLNKGYIK (0)

SDFYIVQQSVVKGHRLASLFSTTNNDFHSQFRRSVNSAFSMSALVQYEPFVDNTTKLFLNQTEKLFAGKP

EGCDFTQWLQFYAFDVIGEITYSKRHGFIEKNEDVEGIVAYLSKLFLYVAP (0)

IGQIPFLDLVFLKNPIYLKLSQWGLFDSTFPVAKFARARMAERLIPELNSDAVL

PTTDIKKQALGQDLLSKFIAAREARPEFMTDTLVQTMAVSMAFAGSETTAITLSAVFYYLLRNKSSYYKL

QKELDEASKAGAFSDYETGLVTFAEAQRFPYLHACIQEAFRMHPAPGLPLERIVPPQGAEIGGHHIKGGT

IVGASAWLIHNRPEIFGDDTSEYRPERWLPDPNANPEDEDKRIKKMSGMMFQFGMGSRTCIGKNISLLEIYKVVPSLLRRFE (0)

IDFQDPSKEWRIINAWFVKQADFNVTFTRRQLVQPEITEKAG*

 

>CYP532A6  Aspergillus oryzae

          GenEMBL BAE66552.1

          74% to 532A4

MTLLAEVFSRASPEYALYILPFISLIYIGHLYFHNGLNRYPGPFLAKFTDLWRFLDVWGRQPHETHIALH

RKHGDVVRIGPNTLSFSSPAATKVIYGLNKGFTKSEFYPVQMTVSKGEPLPSLFSTLDDKFHAELRRSVN

HAFSMSSLVQYEPMVDETTLLFLDQTDRLFATGGKVCDFARWLQFFAFDVIGSITYSKRHGFIEKNEDID

GIVKSLARIFNYAGPVGQMPWLDKVFWKNPIFDAMQKWGLLDNSHPVAIFARQRMMERMSSKAAIDPSSR

SDLLTKFMKAGELRPNFMTEKRVLTMAVSMAFAGSETTAISLAAVFYYLLKTPDYMRRVREELDEAIQNG

TIENRPSGLVSWTESQKLPFLDACIKEAFRIFPAAGLPLERVTPPSGADIAGQFIPGGTIVGCSPWVIHR

REDIFGSDVDTYNPDRWLNASEDKLKIMNGMMLQFGAGSRTCIGKNISLMEIYKLIPSFLRRFDVRLAYP

EQEWRLWNAWFVRQYNFNTVFTPRKIEVQ

 

>CYP532A6  Aspergillus flavus

100% to CYP532A6  Aspergillus oryzae

AFL2G_11872

MTLLAEVFSRASPEYALYILPFISLIYIGHLYFHNGLNRYPGPFLAKFTDLWRFLDVWGRQPHETHIALHRKHGDVVRIG

PNTLSFSSPAATKVIYGLNKGFTKSEFYPVQMTVSKGEPLPSLFSTLDDKFHAELRRSVNHAFSMSSLVQYEPMVDETTL

LFLDQTDRLFATGGKVCDFARWLQFFAFDVIGSITYSKRHGFIEKNEDIDGIVKSLARIFNYAGPVGQMPWLDKVFWKNP

IFDAMQKWGLLDNSHPVAIFARQRMMERMSSKAAIDPSSRSDLLTKFMKAGELRPNFMTEKRVLTMAVSMAFAGSETTAI

SLAAVFYYLLKTPDYMRRVREELDEAIQNGTIENRPSGLVSWTESQKLPFLDACIKEAFRIFPAAGLPLERVTPPSGADI

AGQFIPGGTIVGCSPWVIHRREDIFGSDVDTYNPDRWLNASEDKLKIMNGMMLQFGAGSRTCIGKNISLMEIYKLIPSFL

RRFDVRLAYPEQEWRLWNAWFVRQYNFNTVFTPRKIEVQ*

 

>CYP532A7 Mgr043 Mycosphaerella graminicola

55% to CYP532A2   Magnaporthe grisea

MLIDEVLLPILLDYWPLIVVATTAIHLLRNKYHNGLNQYPAAHPLASYTDWWRYFDVSKRRAQHTHLDAHSKHGDIVRLGPNVLSFSDPKAIKIIYGLNKGMTKSDFYPVQRAVAGGRALPSLFATKDEGYHARYRRCINSAFSMSSLIEYEPLVDSTMNVFIEQTKRRYCDTGLTCKFSQWLQFYAFDVIGELTWSKRLGFVERAEDVDNIVSFVSGFLDYCAPVGQMPWLDFVWEKNPLRLLLQRWGVSKTVFPVTKFALARSAERGSEMDKIRQHGAVEKSTKGIDLLTKFTLAQHDHPDFMTDKQVLASCTSMIFAGSETTAISLSSIFTHLLQNPRAYAALMAELDAATLAGTIEPRSNNRVSWSESQKLPYLDAVIQESFRLHPAPGLILERVVPASGMDILGTHVPGGTIVGCNAWVLHRRPEVFGADVNAFRPERWLEAKPDQLREMKASMFQFGAGARTCIGKNISLLEMYKLVPTFLRNFEVTLEDRVKTTNAWFVRMEFHARFKPRDQATGA

 

>CYP532A8 Fusarium oxysporum

85% to CYP532A3

FOXG_10880

MHSGLVETLLSALAQHWVLVLTSLTVAWLVKNRYHHGLNKYPGPFLASLTDWWRFWDVYGQRPEVTLQKLHAKHGDIVRL

GPNTLSFADPAVLKSIYGLSKGYVKSDFYIVQQSVVKGHRLASLFSTTDNDFHSQFRRCVNSAFSMSALVQYEPFVDNTT

KLFLEQTEKLFAKRADVCDFTQWLQFYAFDVIGEITYSKRHGFIEKNEDVEGIVAYLGKLFLYVAPVGQIPFLDLIFQKN

PIYLKLSQWGLFDSTIAVARFARARMAERLIPELKSVDSLPTSNLKKQPLGQDLLSKFIAAREARPEFMTDTLVQTMAVS

MAFAGSETTAITLSAVFYYLLRNPECYQKLKNELDEAAKAGVFSDYESGLVTFSEAQKFPYLHACVQEAFRMHPAPGLPL

ERIVPPQGAEIGGEFIKGGTIVGVSAWIIHNRPEIFGTDTDKYRPERWLPDPNLDAEEEDKRIKKMTGMMFQFGMGSRTC

IGKNISLLEIYKVVPSLLRRFEINFEDPSKEWRIINAWFVKQTDFNVKFTRRELVQPEFSEKQE*

 

>CYP532A8 Fusarium verticillioides

98% to CYP532A8 Fusarium oxysporum

FVEG_09894

MHSGLVETLLSALAQHWVLVLTSLTVAWLVKNRYHHGLNKYPGPFLASLTDWWRF

WDVYGQRPEVTLQKLHAKHGDIVRLGPNVLSFADPAVLKSIYGLS

KGYVKSDFYIVQQSVVKGHRLASLFSTTDNDFHSQFRRCVNSAFSMSALV

QYEPFVDNTTKLFLEQTEKLFAKRADVCDFTQWLQFYAFDVIGEITYSKR

HGFIEKNEDVEGIVAYLGKLFLYVAPVGQIPFLDLIFQKNPIYLKLSQWGL

FDSTIAVARFARARMAERLIPELKSVDSLPISNLKKQPLGQDLLSKFIA

ARESRPEFMTDTLVQTMAVSMAFAGSETTAITLSAVFYYLLRNPECYQKLK

NELDEAAKAGVFSDYETGLVTFAEAQKFPYLHACVQEAFRMHPAPGLPL

ERIVPPQGAEIGGEFIKGGTIVGVSAWIIHNRPEIFGNDTHVYRPERWLPDP

NLDAEEEDKRIKKMTGMMFQFGMGSRTCIGKNISLLEIYKVVPSLLRR

FEINFEDPSKEWRIINAWFVKQTDFNVKFTRRELVQPEFSEKQE*

 

>CYP532A9 Mycosphaerella fijiensis

70% to CYP532A7 Mycosphaerella graminicola

fgenesh1_pg.C_scaffold_1001379

MLAGELLKALINYWHIVLVALLAAHLASNKYYKGLNKYPGPRLAAFTNWWRYFDVLSRKAERTHLDLHRKHGDIVRLGPN

VLSFADPRAIKIIYGLNKGFTKSDFYIVQQAVAKGERLQSLFSTKDEDYHAKYRRCVSNAFSMSSLVGYEPLVDSALNAF

IEQTRRRYCDTGRSCKFSRWLQFFAFDVIGELTWSKQLGFVQRDEDVEGIVKFVADFLKYAAPVGQMPFLDLIWEKNPLR

LKLQQLGFIKTEFPVSRFALAQNATRAAEIEKIKQGAALDDRSGKGIDFLMRFTQAQHDHPEFMTDKQVLASCNSMIFAG

SETTSISLSSVFYHLIKHPRVYQKLMSELDDAAVNGTITEKNYGKVSWSEAQKLPYLDAVIQESFRMHPAPGLILERVVP

PQGMDILGERIPGGTIVGCNAWVLHRRPEIFGPDVDVFRPERWLEARPEQLREMKATMFQFGAGARTCIGKNISLLEIYK

LVPTFLRNFEVEMAGEEDAEWKTCNAWFITQDFDTKFRPRRIAQSA*

 

>CYP532B-532G # renamed CYP5080A, CYP5078A, CYP5080D, CYP5077A,

CYP5080B, CYP5080C

 

>CYP532H1 Nectria haematococca

fgenesh1_pg.scaffold_58000031

Necha1/scaffold_58:68174-69912

46% to 532A2 with two small deletions compared to 532A2

These deletions are not seen in AP007175.1 Aspergillus oryzae

This gene model seems correct

MILELLIPHSFGVAILWLLAGILLRLLVNKYGQGLNKIPGPWLAGFTDLWRLFIVRGRRAQEVHIELHKK

YGPMVRLGPRAVSVADPEAIKIIYSPSSGYSK (0)

SGFYPVQQALAKGNRLETMFNTANDSYHARLRRSVSNA

YAMSTLVAFEPFVDSTSTEFLKQLKLRFADRPGDEGICDFGAWLQYYAFDVIGELTFSKRLGFVETGRDV

DNIIRDLEGFLNYVSW (0)

IGQIPFLDRLFIKNPIKIWMAKNGLLNSSAPVAEFAKKHVVERQREEESGNSKT

PRRDFLNRFKEARAKDPEFITEQLVLALTVANMFAGSDTTGITMRAVFYYLLRDPSKMDKLLDELARESK

AGRFSREDGLAQWEEVRDLPYLSAVINEALRCHPAVGLTMERIVPPSGVTIAGHFLPGGTIAGCSAWVIH

QNAEVFGSDAADFRPERWIDATPEQRRRMNNCLFSFGAGARTCIGKNISLLELYKLVPTILRRFE (0)

LELVNPETPWKLHNAWFVRQAGFNVRLKERDQII*

 

>CYP532H2 Nectria haematococca

Necha1/scaffold_12:138036-139715

e_gw1.12.581.1

55% to fgenesh1_pg.scaffold_58000031

This gene model seems correct

MFISSFISTHLDILAGLLVLSLLAKLVYNRYGHGISHIPGPFFASLSDLWRLYVVWGRRPDIDHVKLHER

YGKLVRLGPKAISISDPGAIRQIYGLAAGFQKSDFYTVQQTLARGRPLFTLFTSTDTRFHAKLRRSVSSA

YAMSTLVQFEPYVDSTTVELIRQIDERFKDKQESCDLGKWLQFYAFDVIGELTYSKRLGFVDRGVDVEGI

ISSIEWMLDYASV (0)

VGQMPIIDSFLLKNPIRLFLSRLGLIGSTTPMVKFARQRFSERVDLATGQEKPSSRM

DSSSTRRDFLSRFLEAHKKDPDFVTSERVLALTVANIFAGSDTTAISLRSVFYFLLKSSKELERLMAELE

EQKRTGKFKRTDGLVDWEEARDLPVLGAVIKEALRIHPAAGLTLERITPPQGVRISDKFIPGGTIIGCNA

WTIHRDASIFGSDPDRFRPLRWLEASPDQRRLMDNCLFSFGAGSRTCIGKNISLLEMYKLIPALLMRYK (0)

LELPRENSDWKLHNAWFVKQSNFYVRFKTRGYSA*

 

>CYP532H3 Fusarium oxysporum

81% to CYP532H1

FOXG_10225

MILERIVPQSFSAAVLWLIGVILTRLIYNKYGHGLNHVPGPWLAGFTDLWRLLVVRGRR

AQEVHIELHKKYGPAVRLGPRAVSIADTDALKVIYSPSAGWSK (0)

FYPVQQALAKGKRLET

MFNTDNDRYHARLRRSVSNAYAMSTLVTFEPFVDSTSAEFIRQLKLRFSDQTGNAGICDFGAWLQFYAFDVIGELTF SKR

LGFVEKGIDIDNIIRDLESFLNYVSW IGQIPFLDKLFIKNPIKIWLVKYGFLNSSAPVAEFARKHLVERQHEEASDMPKA

PRRDFLNRFKEAHNKDPDFITEQLVLALTVANMFAGSDTT GITLRAVFYYLLKDPPKMESLLKELAAESKAGRFSRDDGL

VQWEEVRDLPYLSAVINEALRCHPAVGLTLERIVPSGGVTLGGHFIPGGSIAGCSPWVIHQDAEVYGADAAEFRPERWIE

ATPEQRKKMNSCLLSFGAGARTCVGKNISLLELYKLVPTILRIFELELVNPDTPWKLHNAWFVRQMGFNVRLKERKTIL*

 

>CYP532H3P Fusarium verticillioides

90% to CYP532H3 Fusarium oxysporum

no gene model, missing C-term

MILEHIVPQSFKAAVPWLTGIILTRLMYNKYGYGLSHVPGPWFAGFTDLWRLLIVRGRRA

QEVHIELHKKYGPAVRLGPQAVSIADTDALKVIYSPRAGWSK ()

FYLVQQALAKGKRLKAIFNTDNDSYHARL*RSVSNAYAMSTLVTFEPFVDSTSAEFIR

QLKLRFSGNTSNAGICDFGALLQFYAFDVIGELTF &

SKRLGFVEKGVDIDNIIRDLEGFLNYVSW (0)

IGQIPFLDKLLIKNPIKIWLVKYGFL ()

NSSAPVAEFARKHLVERQLEEASDVPKAPKDFLNRFKEAHNKDPDFITEQLVLELTVANMFAGSDTTG

 

>CYP532H4 Fusarium oxysporum

60% to CYP532H1 Nectria haematococca

FOXG_16603, AAXH01001105

MILDLFLQHPWAYLTAAVVGLLVTKLLVNKYGNGLNGIPGPALASFTDLWRFLDVYRRRPEVTQIALHEKYGTVVRLGPN

TVSIADPAAIQTIYAHNSGYTKSDFYPVQQTINKSGKRLITLFTSQDEKFHSQLRRSVSNAYAMSTLVQFEPFVDSTTTE

FFKQLDQRYANQNDILDFGTWLQYYAFDVIGELTYSKRLGFVDHGKDVDNIIGNLEWLLNYAAPVGQLPILDSLLLKNPL

RLQLTKWGFTNSSSPVAIFARNRMLARVDPEKLGDMKFDQDNGRRDFLSRFLEANQKDPEFMNNDRVLALTVANMFAGSD

TTAITFRAIFYYLMKNPADMKTLMAELAEEEKAGRFAREDGLVSWNEVRDLPFLNAVVKEALRCHPAAGLMLERIVPARG

LEVNGHHISGGTIVGVNAWVLHRNKDIFGHDADRWRPSRWIEASTEQKRRMENYMFAFGAGSRTCIGKNISLLEMYKMVP

ALLRRYE (0)

LEFPSADNTWHLNN (1)

AWFVKQSNFNVRLRRKHKLSFLPDERQD*

 

>CYP532H4 Fusarium verticillioides

97% to CYP532H4 Fusarium oxysporum

FVEG_13640, AAIM02000185.1

MPCVSPVQQTINKSGKRLITLFTSQDERFHSQLRRSVSNAYAMSTLVQF

EPFVDSTTTEFFKQLDQRYANQNDILDFGTWLQYYAFDVIGELTYSKRLGF

VDHGKDVDNIIGNLEWLLNYAAPVGQLPILDSLLLKNPLRLQLTKWGFTNS

SSPVAIFARNRMLARVDPEKLGDMKFNQDNGRRDFLSRFLEANQKDPEF

MNNDRVLALTVANMFAGSDTTAITFRAIFYYLMKNPDDMKTLMSELAEEEK

AGRFTREDGLVSWNEVRDLPFLNAVVKEALRCHPAAGLMLERIVPARGL

EVNGHHIPGGTIVGVNAWVLHRNKDIFGHDADQWRPSRWIEASTEQKRRM

ENYMFAFGAGSRTCIGKNISLLEMYKMVPALLRRYE (0)

LEFPSADNTWHLNN (1)

AWFVKQSNFNVRLRRKHKVSFLADERQY*

 

>CYP532J1 Nectria haematococca

fgenesh1_pg.scaffold_24000107

Necha1/scaffold_24:254874-256710

47% to fgenesh1_pg.scaffold_58000031

MAIITEITGLIREHLPVIAVVLVLVHLTRNYFTRGVSEVPGPFLARFSNLWRFIDVARGKAEETHLKLHQ

KYGQYVRLGPNLVSVQNLDALKVIYGVNKGYTK (0)

TKFYEPQQQLFNGNPTQTLFTTLDEDFHARIKRPISSAYSMSTLTEFEVFVDKTIHTMIGRLDEFARDGRVCDMAIWLQY (1)

YAFDVIGELTFGKPLGFLEQGKDISNIMLSLERNLDYAGM (0)

VGQMPWLDHLIVKNPIRRLMGGSATGAIAKFARECLDERLERGDTKSEKPLRRDFLTRFLDAKVTHPQ

VVTDRQVFSYTLSNVNAGSDTTAISLRAVLYYLLKNPVIMDKVCKEIEHAHSEGKFSIPVTWKESQELPY

LDAVIKEALRLHPAVGLLLERIVPEGGLQLPDGPFLPAGAVVGINPWVMHHSPIFGEDLDSFIPERWLQV

ANETKQQYDSRKAEMLGATFTFGAGSRTCIGKNISLLEIYKAMPTLLYLFK (0)

IELDEPEKEWETCNAWFVRQKHINVRLTRVR*

 

>CYP532J2 Aspergillus niger

e_gw1.2.1446.1|Aspni1

63% to CYP532J1

MALLTTTLTYLSQHIPSILFTILIIHLARNYFKPGLSRIPGPFFAKITNLWRFVDVARGRAEVTHYQLHQKHGEYVRLGP

NVVSVWNVEALKVIYGIKRGYKKTAFYRVQQQLVKGKPTRTLFTTLDEDLHAKIKRPVSAAYSMSTVTEFEPFVDSTTQT

LFARLDELSEKGCVFDISTWLQYYAFDVIGEMTFSKQLGFLREGRDVEGIMESLEKMFDYAGKIGQMPWLDYVLIKNPLR

QLIRGGSTGAVARFARDRLHERLSQGGSKRDDIHERQKDFLTRFLEAKSIHPDVVDDAQVFSYTTSNVNAGSDTTAISLR

AILYYTLKNPRVFSKLQDELNGALQTGKVTIPVSWKQSQELPYLDAVIKEALRLHPAVGLLLERVVPAEGLQLPNGPFLP

AGTVVGVNPWIIHRHAIFGKEIDRFVPERWLRGNDESEAEFKMRRQNMLGATLTFGAGPRTCIGKNISLLEIYKLIPSLL

LAYKIELSEPEAEWEVVNAWFVRQ

 

>CYP532J3 Aspergillus terreus

72% to CYP532J2 Aspergillus niger

ATEG_04107.1

MAVLSSIYGFVSQHLLALTVLSIVYYLTRNYLTPGVSSVPGPFLAKLSNLWRFVDVARGRPEITQYKLHQKYGDYVRLGP

NVVSVRNPDVVKTIYGINKGYRKTDFYRVQQQLAKGKPTPTLFTTLDEDFHAAIKRPVSSAYSMSTLTEFEPFVDSTIRS

LFVKLDEFVASRKVCDIAIWLQYYAFDVIGHLTFSKPLGFLEHGKDVDGIIGSLEKTLDYAGKIGQMPWLDYVFVKNPLK

QLIQGGSTTAVARFARDRMSERLSQPSEKASAASSQQKDFLTRFLEAKKTYPETVTDAQVFSYTVSNMNAGSDTTAISLR

AILYYTLKTPQVKAKLMAELLDALHTGKLTLPVSWKQSQELPYLDAVVKEALRLHPAVGLLLERIVPAGGLQLPNGPFLP

PGTIVGINPWIIHRHAVFGSDVDSFVPERWLRGEDETEAAFQARRQDMLRATLTFGAGPRTCIGKNISLLEIYKLIPSLL

LTYEIELDDPTTEWEIINAWFVRQKKIDIRLGRRYLGEGRVGAQTGRQCDV

 

>CYP532K1 Mgr001 Mycosphaerella graminicola

45% to CYP532J1   Nectria haematococca (yellow part omitted)

42% to 532A6, 45% TO 5077A1

Families CYP532 and CYP5077 overlap, cyan from JGI model e_gw1.12.214.1|Mycgr3

MILTALYGAFEALIYGALPTILIIVVGGSIVLGYTT

LSDIPGPFYAKFTDYWRFRVVGKRNSHETYLKLHEEYGDFVRIGPNCVSISRPDVIPLIYGINVKATKSDFYTVWQNVVNGKRTASLVFTTDEAHHATMKRPIANAYSLSTLVEFEPLIDSTTAVFLSRLDSQFASTGAVCDLGTWLQWYAFDVIGELTLSKRLGFLEKGEDVENIAANVARNFDRCSSLGQMPWLDLIYKHPYFQALFLKSPVNPIIAFGQRRLQERLDDNEDTPPP

TDLGITDPSLAAKVLHTTL

PSKPDFLSRFLTLHETQPEIVTENTLLAYLFMNINAGSDTIASTVRALFYHLLRHPSTLTTLMTELDAAHEAGNLTLPLPTWSEVQPLPYLSAVVKESLRLNPALALPLERIVPAAGITINGTFFPPNTVIGVNPWVLHRDTRIFGSDAKEWNPSRWIDGDAERTRYMESHLLSFGAGKRTCLGRNIAILELSKVVPAILCRYKMRLEDEGREWKVVNSWAVRQEGLEVLLERR

 

>CYP533A1 N. crassa AABX01000277.1 cont3.42 NCU01134.1 (version3)

41700 MLKFFMGDFDIGPLSNMVTSNSSATRASSAPANGFQFIQQSVDT

      VAGTAIGLFIAYSAYKNLSEAYRRGDPNRKAPLPPSPPGDFLLG

      HYRHVPENESFRKYLEWSKKY (1) 42026

42089 NSDVLYFETFGTKWVVLNSYEAAHELLEKRGRKYSDRPRFVMFEE (2) 42223

42284 MGWAPTLTWLRWGPQWGLHRKVLAPPFAKTGCVSFQPLQRKQAM

      IMCKNLIENPEEWMSAVTHFAVAIMFKIGYGIDVDTPTDEWVKL

      AAEASEAVGKAGAPASSIMDRIPWSKFLFLATI (0) 42646

42717 RLKYAHENKKVIQNITERPFNASMSDY (0) 42797

42850 HERDLDPTRKLKECFVHRMYHLRGEDARRNRQNVYYEEDIKGGA

      ATMLIAGNDTTASTLNLIILYLTKHPRVQRRAQAEVDYILLTTG

      VPTEPVEDYRPSTPAPWASSSSNRAGIIRLPTLEDIPKFKYVNL

      ILQEVYRINPLSPLGIPHASVKDSSDIDPDGDDTYNGMRIPCGT

      IVYPNVWAMNRDEKRYREPERFFPERYLPKEEGGWGEPLPVGNF

      GFGRRVCVGQYLAENSLLIAIAMMLATIEFDLPIGPDGNLKDFE

      PQFSHKGQS (2) 43668

43748 IVLPFKVSIKPRSPMVEELLDRHILVAKMAEKAAEDGKKGGPTPSA* 43888

 

>CYP533B1 Magnaporthe grisea AACU02000566.1 revised

MMTSTAGPYATTSLLNIGAVLVGLILVCKLASVVPSLRLGGWLSLVRR

RKLPLPPGPPRNFFLGHYRTVPFEAPFKKYAEWGKQY (1)

QSDVLFFSAFGRPWMVLNSLEAAVDLLEKRGHKYSDRPRFVLWEL (2)

MGWAPTLTWLRWSPK

MLQHRRMLQPPFSRSRVGQFKPVQKREAVRAALCMVRDPEHWAKAATRFA

VGVVLDISYGIELKWTGSCNNNNPYVALLDDASTAIGNAGPPASSIVDRW

PLARHLPAWLPFMER

LRYARKWRSAIVQVTNFPF

SAALREMLSTSIEKTTKPSFVRERLDVFRRNQQAGVDNDFGMDDIKGSAA

TILIAGSDT (0)

TATTVRLFILYMMQNPRVQKRARTEIDRAIGTTTPTEMRRLPTWGDMNKLPYLAL

VLQEVYRSNPLSPLGIPHATLEDDEYRGMLIPKGTVVYPNAWAMAHDPLV

YSAPDEFLPERYMPRGSITIGQTKDDAAPGRGESLPIGNFGFGRRICVGR

TLAENSLLIVFATILATLEIGPPGGEDGQEDQDVQWSFRGQAYPLPFRCS

IVPRSDMAIRLLEDSAGVPEAT*

 

>CYP534A1 N. crassa AABX01000272.1 cont3.221 NCU04234.1 (version3)

10018 MRLTPLVTLLGVFLAWATYKIYAGFRSNIAKARKTGYPVLIT (1) 9893

 9782 PIYPLSPLWLTTRHFLLPILTRLLPERLYASYVFVMTPDWEY

      HPSPRSHFTRLGSDTF

VVASWNGLACYTSDAAVISQVMSRREAFPKDTRQYGILEIFGSNVVTTEG

QVWRMHRKVTSASFNERNAGHTFREAVRQTRGMVGGWFSSSSSSSSSSSS

SGSQEEGTGRREGDVEDMTGIITTLEHDTMRWALNIIGYVGFGLKLLWPH

ESMPKDMDPKLAKYGSLQPPYGYSMTFADSLATVLERIVILLLIPSAVLK

RIPERWGSWGSKLHEAWEAKVNYSKYMNAFLAEKVQDIRAGVQEREGMNI

MGQLVRSKYDPSSSEKNDGATMKLEDSEIMGNAFIMTLAGHETTANVIHF

AIVELAMHPEVQRRVQRDIDAIFGRDSDPETWDYEKSINALLASYIGAVI

NETLRLIPPVVVTPKRVADTDQWVQDGGQRHPMPAMMPVMPVISAVQRSR

RYWPALHDKTTKKGDTDDLDEWHPDRWYLPSMSQGQPSSDDEKGEGKQQQ

EENYGGYQGTDTSPTLFRPVRGSFIPFSDGARSCLGRRIAMVEMVAAIAV

LFQKYSVELAVDASDEEVQMMVPEQRRHVYAKAQEKAKETVQQAQSVVTL

KLTGGRHVPVRFVRRGKERFMGNEASC* 7873

 

>CYP534B1 old name = CYP588A1 Magnaporthe grisea MG06872.4  44% to CYP534A1 AACU01000608 cont2.1276

MSGIRVISAVAVAWALAALFTGFWKLQRNIASAKRTGFNYVICLYAAMPH

LCNADELPASVLTKDWAFKIDHGEIWRKLGDTFMVITPWTLIVYTRCPEA

IQQITSRRDDFPKAVEQYGVLEMLGANIVTTEGAVWKMHRKVTSSLFNEV

SAANAFEETVRQTQSMLRIWRGHSDDDGQDGQVAADGRTISSLAHDTMTL

ALNIIGTIGFGLRFLWPGEKASDNQDPRMNKYTQSEKDSNYLMTFPNATA

GMLENIFAILLVPSILLRHLPFEWARSAHESNVTYVRYMRELVKDKTEDL

HKGVPKTDEHMDIVGALVRAKDDQKKQQDVGKTDIIPPGLQLTDDHIISN

AFIMMLAGHETSANMLHFAMTLLAAHPSSQRAMQADLDRLFPDGDEEATG

WRFENCINDLLTSMPGAVLNETLRFLPPVVVIPKQVSPSRDQVLRVDGDS

VEHVLPAGTIVSVTVVSAARNEKYWPPNEAGESDVHEWIPERWFRASSSS

SPPPVETAQRRTGDSRAADEDVTDDEGDGLETTSAGADKPMFRPVRGLFV

PFSDGARSCLGRRIARVETVTALAVVFRKYSMELAVDEWADDEAVKRMND

KERAELYHKAIARCMETLKTATSTLTLKLPKDQCVPVRVVRRGKESNQGR

IEYISAPPRRVKQSQPPHPKNPGALVMMPLGLKIDVENE*

 

>CYP534C1 old name = 588B1 Fusarium graminearum FG09916.1 AACM01000411 FGcontig1.411_scaffold7

MQTVLLLVGSAILYAVYKYVSGLRCNIEAAKKSGLPYIVAPWSPISVPWV

LGYKGLVPIIKLLPKPLWEEWLEVLTPGFSYNTRHDAFARHGDVFIIVSP

NMLYMMTCNAEAIRQITSRREHFPKYVESYEILRVFGNNVLTSEGAIWRN

HRKVTSASFNERNAALVFQEAIHQAQGLVNMWMGPGGEDSGTINTLDEDT

MRLALHIIAYVGFGLKLLWPGESLPAKANLKMAKYGSPEATAGHKMSFVA

TIATALECILLILLVPRWILAMMPFKKTRLAADSHDEFSKYMHELMDEKI

EEARKGEHVDGMDLMGQLVRSSYATGIGEGMQSKKGALSRDEIQGNAFIM

LVAGHETTAGALHFILLELANNPASQRRLQNDIDEIFGGKEPSSWDYDAL

IYPMMASMLGACMNETLRMTPAVVEIPKKVPPKQEQVITIDGNSHLIPGS

TIISLVAVSIHRNPRYWPGRPSHIYAGEDDINDWVPERWYRSYEAAAKPT

ESDIAAPENEDFGGYKGPDTSSQLFRPERGAYIPFSDGPRSCLGRRIAQV

EIIAALAVLFRDYSLELGVDEWANDEEIERMNKEGRRKVYAKAQSASRAK

ILGATSLLTLKMNGDYVPVRMVKRGQERVQ

CGINGDERVAKKCRVARVTR

LPAHDYFSNSKFAARKKSQVVKHADWCKVTVIPPK*

 

>CYP534C2 Nectria haematococca

e_gw1.6.655.1

Necha1/scaffold_6:586178-588240

71% to 534C1 = 0ld 588B1 F. gram. may be too long, add one extra phase 1 intron after PRWLL

Note: the region between PERW and heme motifs is about 40 aa longer than usual

And the C-terminal tail after the heme is about 40 aa longer than usual

MQSVLILVGAALAYAVYTYISSLRQNIAEAKRSGLPYIVA (1)

PCSPIFLPWQLTFKLWTPIIKLFPKPWWERWLE (2)

VLTPGFSYRTRHDGFAKYGDIFMVVSPKLIITMVCNAEAVHQITARREHFPKYIETYEILQQFGDNV

LTTEGAVWRMHRKVTSASFNEKNAALVFKESIHQAKGMVTMWMGSNEDDSGTIHTLDRDTMRLALNIIGY

VGFGFRSLWPGETLPADVNPRLAKYSSLEPSAGHKLSFVSTVAILLEYILMLLLIPRWLL (1)

RLMPFKKAHLAADAHEDWCKYMQEMMDEKIQEAQRGERVEGMDLMGQLVRSSYGTGETQGKQ

GPEPKKGSLTRDEIQGNAFIMLVAGHETTANAMHFILLELANNPASQRRLQKDIDDILGGRDPSSWEYEG

LVNSMMASMLGACMNETLRMIPAVVEIPKKVTPGQDQVITVDGKRHLLPRGTVVSLVAVSIQRNPRYWPA

RPSKLHPGEGDINDWVPERWYRKSEDPVAKETDTEEPEVEDFGGYGGSDISSQLFRPERGAYIPFSDGPR

SCLGRRIAQVEIIAALAVIFREHSIELAVDEWASDEQVESMDREQRREVYAKAQTTSRNKLLGATSVLTL

KMNDDHVPVRLVRRGQERFTSWI*

 

>CYP534C3P Fusarium oxysporum

60% to CYP534C1 Fusarium graminearum & = frameshift

FOXG_05507

ILLI*ITILYLIYTFTSNPRQTTVLSKESGVSYTIA ()

VGAGGNDSGTITTLNQNIMRPTPNFIACPGFGLY*IW

PGESLPSKADPRMTKYGSPKPIADHELSVATAIATAPENILMLPPYHAGFSV (1)

SLMPFKRTQIAAKSSE*FGEYMQELMDENIIEARRGERIDGMDLMG*LERSS

YRTDVGPHPQGKELNKGALSQDDIRGNTFIMLAAGQETSAGVLQFILLELANNP VSQRSL

HGAIDETCGDGDPSLWDYENLINPKMASMLGACINQTLRMTPAVVEIPEAVPLN*GQIVT

IGGKSH &

VPSKTFISSGAVSAHRNPRCWPGRPSLINDGKDDINDWVPERWYRNSQESTARS

TESGCQPRDEEFGSAATSTLLFRLEGDSFIPFSDGPRSCLGRRISQGEIIATLAVLFKDH

SLELAVDDWASDEQ MDREARREVYAKAQSSSRKKIRGATSVLTLKIRDGHVLVRLVK

 

>CYP534C3P Fusarium verticillioides

61% to CYP534C3P Fusarium oxysporum

no gene model

VFTLRVPYREGHEGFTSRRDVFLVESPGILFLVV*NADAIRKIFSRREHIPKCAPKHEKL

RQFSYDVFAAEGEIWRYKEMQYRVLSKIDTALLLRSLIQKAQVLVEM

GTGERDSGTITTLNQNRMRQTTNFIPFPGFGIY*ISPG*SLLSKADPKMTKYRYLRSI &

IATTIAAASTNILVLP & PEHTGL

TIVLLPFARTQIAGKSSREFG*YMEKLIDERIIEVRQSERTDDM &

LQFNC*SWQTTQVPQRSLRGAINEICGDGYPSLCDYENLINISMASMSGVCINDTP*MTY

ISAVFEIPVEVSPKQGQTITIDNKSHL

SKTFMPSCRNPRWWPGRLSLINGCKDDINEWVSELRHRKSRESIARSTGS

GRQPRNEEFGSPATSTHLFRPEVDSFI

PFSEGPRSRLGRRISEQGIIATLVVLIKEYFLELATDDWTSDEK

MNREARRK VYAKAQSSSNTK

 

>CYP534D1 Mgr039 Mycosphaerella graminicola

39% to CYP534C1, 41% to 534A1, 41% to 534C2

MFLNLVLATLGLTVLYIANCYRCF

RINLASAKQSGVPYICLPVYTFDRFWLVTHKLWLPFIGLLPKAWTESWIVYLSPEY

IWTKKYDLYKDLDTDVVILVAPGANTMIVADPEANTQITTRRNDFPKPIWLYTSLDLFGKNVVSTEGANWRHHRKITSPP

FSEKNNVLVWKESLTQAECMMTEWVGTGSDESKSVWSVASETMRLSLHIISRAGFGVRLHWPHENRDDSIPEGHTMTYKG

ALETLLHRILTVILTPRWMLANSPLEIHKLAYRGLTEWGQYMREMYTQKREEVEQGGGDREGMDLMGALVEGAGISSSSK

KSQQLLTDDEILGNAFVFILAGHETAANTLHFSILFLAMKMASQRHLQKDLDDLFGDRPASEWSYDEDLPKLFGNMCGAV

MNEQLRLIPPVTGIPKFVDTKGPQGLRVGDKHVTVPAGCYVTLNTIAVHRNPKYWPHTSEQDLLEFRPERWLLDPSKTAS

SNHADDEGYQVEEGMEFDQTDKRPDTAPTLFRPAKGAYIPFSDGYRSCLGRRFAQVEILAVLAVIFKTWTVELDVSAYMS

DAELKTASEEKKKAAWAKADKKARQLLNDHMGTIITIQIRGDKVPFKFVKRGKENFKW

 

>CYP534D2 Mycosphaerella fijiensis

67% to CYP534D1 Mycosphaerella graminicola

e_gw1.8.61.1

MIPYAVLVGCIAVVVTYIANTVRCFQKNLALAKQSGIPYIAIPIYTFNRFWLVTHKLWLPFIRKLPHSWTKDWIDFLVPE

FPWDKKYEGLFKAPGTDIILLVAPGANTLLVADADCTSQITARRNDFPKPTWLYTSVDLFGKNVVSTEGANWRHHRKITS

PPFTEKNNCLVWKESLEQAESMMAGWVGGNKTDSGTIWTVATEAMRLSLHVISRAGFGVKLFWPHEQSVTAIPPGHTMTY

KDALETLLHSLIPLMLLPRWLMKRLPSEGLKRAERSLTEWGQYMREMYEEKRKEVKRGESTEGMDLMGALVKGSGQTEDV

LNSDVEKAQAKQLLTDEEIMGNSFVFILAGHETAANTIHFSTLFLAMHMISQKHLQDDLDRILGDRPVSEWDYDKDMPKL

FGSMCGAVMNEELRLVAPVVGIPKCTAKDKPQGLNLNGRHVVVPPGCYVTLNTAAVHRNPQYWPHTSLEDVSEFRPERWL

LDPSKTNSNTEDDTYDKEEGLDLDGPDQRPDTAASLYRPPKGAYVPFSEGYRSCLGRRFAQVEILAVLAVMFKTWSVELD

VSDYLSDSEFEKASQAEKIAAWEKADARARELLRNGMGTVITIQMRAGKVPFRFVKRGNERFGEILEISGLRRAKGDW*

 

>CYP535A1 N. crassa AABX01000066.1 cont3.39 NCU00987.1 (version3)

1314 MGAATLIYEARWLLASVVLAIWAVLKTKQYYRLRHFKGPFGTGWFEIWHG

     LAYLSGHPHLKFKEATDKY (1) 1520

1580 GPIARIGPNELMTTSLELLAHINGARNRYTR

     TRWFYIAARFQPNSDNILSELDDNMHTKRRAQMAGG (0) 1780

1848 YSGKENRELESSVD

IHVAELVQLIRTKYRSTEIKTNPFDLAQKAQYLALDVISHIGFGEPFGML

LSDQDVNGYIKHADQGMNAITYLWGLGLTELVLDTPLMHLLGPSDTAKTG

FGAMTANTRKIISKRLEDDPGMTKGSDMLASWFRHGLDKEQLVTESILQL

LAGAETAATSMRCIMLYLVTNPRVYAKLQAEVDAAVKDGRAPPYPSVVSN

ATQRQLPYLWACCKEGMRMHPPVTNSSQKKVPPEGDAVTVEGKTVYLPGG

TNIGYCVWGLNRDQKIFGGDADCYRPERWLNEEDPKKLAQMNLVHEMLFS

     YGKFQCLGKPIALMEIGKAIFG (0) 2855

2934 LMRNFDWSVTNAAEPWKEMNAGALFLTHDFLVEATERSVS* 3056

 

>CYP535B1 old name = CYP571B1 Magnaporthe grisea MG04404.4 

41% to 527C1 45% to 535A1

AACU01001103 cont2.830

MRCRQPDNVRLIQKVSSDRSATADANLPPQLIKQSLLRMGVFAQLYDQRW

GLALLAVGFYVVSKIHAYTKLRAFKGPVGTGFFNLWNSWALFSQESHLRY

KEACEKYAPGSIVRIGPNDLLTSSPELLMYMSGVRSPYYRSSWFYKSSRP

RPGIDNVFSSSGPEGEATHTRKRQQLAPGYAGKESPLMESSVDQQVTNLV

ALLRERYRSSEARVKPVDMGDKLSFFTLDVISALSLGRAFGDLANDADMH

GYMEATEAAFGYLNALVASPFGMMMQIPLVHRLVGPQETDATGVGKIVCM

ARQNLKERLRHDTKQKSDMVASFVRHGLSFEELVTEGQFQIVAGSDTTAA

ALKGVLLFLLSDRRVYHKLQAEVDAAAQRLALGPEQVIVDGDARALPYLQ

AVVKEGMRCHPPVTLPIPKMVPPAGDTVVVDGREVFLPGGTHVSYAAWAL

HVREDVYGEDAACYRPERWLAEEDPDRLARMQRTHELNFGYGKYQCLGKQ

LALMEVNKVVFQLLRNFDLALTNPIHGWKKVNFLGIFSPGEMLVTVHERV

ADAARKAE*

 

>CYP535C1 old name = CYP571A1 Magnaporthe grisea MG08500.4 

42% to 535A1 cyan too long AACU01001266 cont2.1600

METLYQGLKSLGVQAIVGIAIASYAVWTIVSYRRLSHIPGPRWTGVSNIP

HSLAFLSGECHKWYHAASNKHGKHFWFLLNPKTSRILTRVGPNSLITSSP

ELWARLNAVRSPYTRTEWFYRGCRLEPGMDNVFTMTNDQEHERRRKQVAS

GYSGKENAKLEADIDANVSALLSLIRSYCTRPGEPSKPMDLAQKIPMFTL

DVISSVGFGTAFGLLAADSDLNGYMASASSGLRINNAIIALGWAVRRFVD

LPIVCKLGPSAKDSYGFGKMIGTVYEAVDRRVRAGDLDKRSDMLASFMRH

GLRGEALRAETLEQVIAGSDTTAAAIRGIMLLLLTSPRVFARLREEVDDA

VRRGGAPQSPEVVAKAQADALPYLQAVIREGMRVKLPVVCFFPRDIPAGG

ETWEVDGEQVFIPGGTDVGYSGNAMHHDKGIYGQDAASFRPERWLDKGAN

LPLMTKTNDLIFGHGKWQCLGKSVAMFELGKVIFELVRNFDWTVENPEKA

WDLHNYNGLMHIENLWVQVQERS*

 

>CYP535D1 Aspergillus nidulans AN9296.1 40% to 527C1 N-term does not match

MPLATLREPAIPLLCGLLALYVLRVIMTYAKLMRFRGPAWTGISNWPHSI

AMLRGSCHEFYAQANEKYGTRTWQPCLPILTVLNRARPNRPSRPSSAHHL

VSGGLDARQQQAWLQALRLTPADSVFGAKYSGRENTDLEFSVDKQLQNLL

DLIRAKYVSSCEQAVPMNLAKKVQYFTLDVISSVGLGKAFGMLEADRDVD

QYLQSSDEGLAIGNAALALGFSNINQAPFIGKFFAPSPKDNNGFGRLMTT

CFRFVDERAASATDKRSDMLASFIRHGLSGEELRTEALEQILAGSDTTAG

AIRGTLLHLMTNPRVYVKLQREIDDAVHRGLAPSAGQGLITAAQAKQLPY

LQAVIREALRVWPPVSNIFPRDVPKDGDTVVVNGQSIFLPGGVCIGYSAY

AMHRSEKIYGKDAKAFRPERWLLEPNPTKLALMVRTNDLIFGHGKFQCLG

KPVAQVEIGKMIFELLRNFDLALLNPTHPWDARNNLGLFTISNMWVQVTE

RTAGLL*

 

>CYP535D2 Nectria haematococca

e_gw1.4.832.1

Necha1/scaffold_4:327672-329378

57% to 535D1 Aspergillus nidulans, 47% to 535C1, 44% to 535A1, 41% to 570C2

This gene model seems correct

MAVVAVLLDNLVPLLLCCLGLFIVRKLVVYNKLKQFGGPRWTGFTDWPHSKAMLQNRCHEWYEEANHKY (1)

GPIARVAPTVLVTSSPEVWMHVNNKPGYKRSDWYYNACRIEYRKDNVFSQTDNDKHDQRRKQMAPG (0)

YSGRENLELEKSIDLRVQELIDLIRSKYLSTQDLIVPVDLAQKIQYLTLDAISSISFGKEFGMLRSDSDVDG

YLQSSEEGLAAGNFALALGISWMAHAPVIGAHIAPSPKDDTGFGKMMNACFRCVDERAANPTDKRSDMLA

SFIRHGLSGAELRSEALEQLIAGADTTSTGIRGALLYIITNPRVYSKLQKEVDNAVQSGQVSEGIIPLSE

SKQLPYLQAVIREALRMWPPAVNIFSRDTPPGGDTVTVNGESIFLPGGVSIGYSGHGIHHSKEVYGEDAK

VFRPERWLNADPEKLAAMVRTSELVFGHGKFQCLGKPVAQLELGKTIFE (0)

LMRHFDLALVNPTKPWDARNSLGLFTISDMWVQAMDRTNESKA*

 

>CYP535D3P Fusarium oxysporum

seq. runs off the end of the contig

75% to CYP535D2

FOXG_17443

MGLFASVSEHRELLVCALLGLFVIKKLVVYSKLRQFGGPRWTGFSDWPHSWAMLQDRCHELYEQANLKH (1)

GPIARVAPNILITSSPELWIHVNNKPGYKRSDWYYNACRIEYRRDNVFSQTDNQKHEQRRKQMAPG (0)

YSGRENPHLENSVDERVQEFLDLI &

RNKYLSSDTQAIPMDVAQKVQYLTLDIISGIGFGKPFGMVQKDSDVDEYLKSSEE

 

>CYP535E1  Aspergillus oryzae

          GenEMBL BAE61757.1

          46% to 535D1, 49% to 535C1 Mg, 47% to 535A1

          52% to 535D2, 44% to 535B1 Mg

          revised 3/18/2009

MGLVELVLETRWLIVGSLFILYFLKKFQAYYRLRKFKGPLSCGFSHFLHTKAVLSLRCERWYKEMTDQYG

SIVRIGPNALITSSPELWAHINAVRSPYKRSDWYYHAARFKPGEDHVFSETNNERHDRRRKQMLMGYSGK

ENLSLESDIDLRVLDFLDLIRNRYLSTEDCLKPMDLAKKVQYLTLDVISTVGLGNSFGMLKADKDVNDYI

KSGEEGLWVSNFLMGTGLHWIMQIEWVGRLLGPSTDDLKGFGKMMATTGQMVAKRLQSPTNARSDMLASF

IRHGLIGNELWMEAFEQVLAGSDTTASGIRGILLCLLSNPRVYKKLQAEIDGAVQDGRAPSTGIISDAQL

RRLEYLQAVIREGLRIFVPVVNIFARDVPPEGDEVIVDGESVQIPGGTWIGYSALGMHLNKATYGEDAEV

FRPERWLIDDKDHLANMTRVNDLIFGYGRWKCLGQTVALIEIGKTIFE

ALRNFDWALANPEQPWRRKNVNGLFAVSDMWVTVTAREQLGSCL*

 

>CYP535E1  Aspergillus flavus

99% to CYP535E1  Aspergillus oryzae

AFL2G_09395

MGLVELVLETRWLVVGSLFILYFLKKFQAYYRLRKFKGPLSCGFSHFLHTKAVLSLRCERWYKEMTDQYGSIVRIGPNAL

ITSSPELWAHINAVRSPYKRSDWYYHAARFKPGEDHVFSETNNERHDRRRKQMLMGYSGKENLSLESDIDLRVLDFLDLI

RNRYLSTEDCLKPMDLAKKVQYLTLDVISTVGLGNSFGMLKADKDVNDYIKSGEEGLWVSNFLMGTGLHWIMQIEWVGRL

LGPSTDDLKGFGKMMATTGQMVAKRLQSPTNARSDMLASFIRHGLIGNELWMEAFEQVLAGSDTTASGIRGILLCLLSNP

RVYKKLQAEIDGAVQDGRAPSTGIISDAQLRRLEYLQAVIREGLRIFVPVVNIFARDVPPEGDEVIVDGESVQIPGGTWI

GYSALGMHLNKATYGEDAEVFRPERWLNDDKDHLANMTRVNDLIFGYGRWKCLGQTVALIEIGKTIFE

ALRNFDWALANPEQPWRRKNVNGLFAVSDMWVTVTAREQLGSCL*

 

>CYP536A1 N. crassa AABX01000239.1 cont3.377 NCU06526.1 (version3)

11364 MGVVLKVKDGKVSPTRVLVGATATVLASYWLYILLFAIVIRAASKRYASP

      LRKYPGPFLASCSRLWKVLSTASGRTHLDHIELHRKYGPVVRIAPNEVSV

      ASPEAARTLLSAGKRFFKTPFYSVFPPAENPDIFTEIREDAHAQKKRVAN

      VPYSMAAMQQLSPFIDDTIELLVRKIDEHIAQHKGVFDLGDYLHYFAFDV

      LGEVAFSRSFGFLAQGRDVDNAIKTIDKSQTYNGIVGQVPELDHLLRRNP

      LWRSIPWLSTKNALITRMALEEMGRRQPFNKDTAVLQTGDTRQDLMASLI

      LGHLKSPEKFGVGDVFAVAHGAI (2) 10399

10314 FAGSDSTASTMQSFFYHVLSSPSTYQS

      ILYEIQSAVATGVIPATGNLTWNEAQTLPYLQACLKEAMRLRPA

      VGLNITRFVPPEGAELDGHFFPGGTSIAVNGWVLHRDKLTFGND

      ADEWRPERWLENEEEARRMERYMFQ (0) 9895

 9825 FGGGSHLCIGRNLALLEINKVVPRLLRDYRFELAHPGRELKATA

      SFFVVQSGLEVFVRRA* 9643

 

>CYP536A2 Magnaporthe grisea MG05235.4  70% to CYP536A1 AACU01000803 cont2.971

MDVNVETSAKAPLVSGLAGFILDNWAIIISAALVIRALYRRYVSPLRKYP

GPFLASISRLWKVISTASSQTQFDHIRLHEKYGPVVRIAPDEVSLASPEA

ARLLLSAGKRFTKTDFYSVFPPPENPDIFTEVREDVHARIKKVANVPYSM

AAMQQLSPFIDDSIEALAHKLDGIADEPTDDRDGPRVDLGLWLHWFAFDV

LGEVAFSRSFGFIAEGKDVDGAIETIDRSQRYNGIVGQVPFVDHLLRRNP

LWKFVPSFNTANALITKIAMSELARRKPFDKESEGKWRGGDGRQDLLASL

IKGHLKDPERFSEGDVFAVAHGAIFAGSDSTASTMQSFFWHCFSDPRVHE

TLLNEINSAVAGGHIPSSGNISWSDAQTLSYFQACLKEAMRMRPAVGLNI

TRYVPPEGAELGGHFFRGGTRVAVNGWVLHRDKDVFGDDAKDFRPERWLE

DAENAKRMDRYMFQFGGGAHVCIGRNLALLEMNKVMPRLLRDYNLHLVNP

GKPLKATSSFFVVQSGLDVYISRKKK*

 

>CYP536A3 Nectria haematococca

fgenesh1_pg.scaffold_32000050

Necha1/scaffold_32:150926-152616

52% to 536A2 Magnporthe grisea

Do not remove first small intron

MALLGNIPVTLSVVSTARTFATQHLFACLIAFILIRCLYKRYFLPLRHFPGPPLASVSRLWR (1)

VWSVWTGKNEEHYISAHRKY (1)

GPVVRTAPNELSFSSATTALDIFKTGKGLDKTDFYGVFPPLYERDIFTEARESVHRVKKRYASPGFSL

QAMQQSTPRIEEAERLLLCKLDEYAHGNKTCDLGDWLHFFAFDVLGEAAFSTRFGFLEAGFDVGGAMESI

NRSNRYSGIVGQIPGLDPFLRRNPLRGYISYFASKMPLITRIALDQLESRVKPGNQKFAKFTDLLNSTMQ

AQKQNPEALSHGDVFGIAHGAIFAGSDSTASTMQSFMWHVLSQPRVHNALAKEIMSASLSEMVQYDESQK

LHYFQACLKEAMRVSPAVGLNISRRVPPTGAEIGGLKLSGGTEVAVNGWVVHRDKQVFGQDADNFRPERW

LEADKDTLR (0)

FGGGSHVCLGRHLALLEMSKVLPELFRRYHLQLVNPDQPLQRSSTFFVVQSGLHVHLKLREMP*

 

>CYP536A4 Mycosphaerella fijiensis

58% to CYP536A2   Magnaporthe grisea

estExt_Genewise1Plus.C_11313

MLKTYWLLLIPTILLVRFLYYRYASSLRHYPGPILASGSRAWKAVWSVWSGHTEIDHIKVHEKYGPVVRIAPNELSFASP

LVAKEVLSAGKGFHKTDFYGVFPPPENPDIFTETREAVHAAKKRYAANPYSMATMHTMAHYIEDTERLFAKKLDAMCASK

DQYCNFGNWLHYFAFDVLGEIAFSRKFGFLESGYDLEKAIKTIDDMQWYDGIIGQIPEWDFAFRRNPLWKLIPSLNTANF

LITRMALQEMDQRKKLGSKELGRKDLLSQLFAAHEKAPDVFKEGDVFAVAHGAIFAGSDSTASTMQSFTHCVLRDPRIYA

KLQKELDEATASGRLSEMPQWNEAQALPYFQACLNEAMRLRPAVGLDITRLIPAEGAEIDGHKLPGGTRVALNAWVLHRD

EDIFGIDSKSFRPERWLDGDAKMMQRYMFQFGGGAHLCIGKNLALLEMNKTLPLLFRDYDFQLLRPQEELKYHSTFFVVH

EGLEVRISKRTTRP*

 

>CYP537A1 N. crassa AABX01000723.1 AABX01000717.1 revised

57% to CYP537A4

521086 MTLLLLLPIIIFLLHNLIYKPLTSPLSSLPGPLYTKFTSLVLK

       YHELRANRTRWIHALHVRYGPVVRIAPNEVSFASREGVKEIYC

       SGGSGFDKSGWYDLFKIYGRR (2) 521406

521667 TMFTFLKKDD (0) 521696

521762 HAKRKRILADRYANSNVMRQAPLSGIIERADR

       VVKRCAESLDGSLDLY (0)

       AVHSYAYDCSIRTTCFHPYGTDXLRN

       QQDEEIMREITFDDSLQ (1)

    97 NRQLSYYSPTLHKAISKILYICGIK

       PRETPLADKFILDTAAKTDAAPFTLLDRLH (1)

       NSKIAQ    

       IDQLDIAAELLDHMVAGIDTTGDALCFLMWELSQPRSRQIQTKL

       REELLSHPSSDINFDKLPYLDAVVMEGLRCFPAIPMSLPRIVPR

       GGKTIDGYFLPEGTTASCQAYSMHRIDMEAWGPDPDAFRPERWF

       EEKGDAARKKLFFAFANGARGCIGKH (2) 801

   907 LALAEMKILLREVYSKFTTVPDKTMTEE

       DMEMEDQLISTRPAGLKCLLKFEPIGGQ* 1077

 

>CYP537A2 Fusarium graminearum FG05426.1 AACM01000224 FGcontig1.224_scaffold3

MFILSP

SLISGAAALFLVAYIITRLSSATAKIPGPFVSNFTSLVLKWQELNANRTV

YIHELHKRYGPVVRVAPNEVSFTSIAAVKEIYGSGGSGYDKTEFYNLFQV

YGKRTMFSTLVKGDHAKRRRMIGDRYANSNVMKAAPLAGIKERSSKFLQH

CVESPERTADVF (0)

ALHAYACDCVTHQLFHPYGSNCLQDKQ

DEEMMHQVAADDSLQNRLVQYYSPVLHNLGSKVLASFA

KPRSIPLADNFVIENAQQNGAASFTVLNRLKEKDSVLDTMDMAAECLDHM

AAGIDTTGDVLCFLMWELSQPRSIKYQRLLREEFLRKTDMPFDELPMLDA

VLNEGLRCFPAIPMSLPRYVPQGGRVIDGFTLAEKTVVSCQAMSVHRIND

DVFPEPDKFHPERWLAAEGDADRRRHQWAFSSGGRGCVGKHLAMVEMKTL

LRDVYSRYETEPDESMKSESMVMDDQLISSRPLGKRCLLKFVPVKD*

 

>CYP537A3 Magnaporthe grisea AACU02000684.1 revised 2X

AACU01001722  58% TO 537A1

MLLFSPSITAIILGVCAAAYLIRCLRSPLRKVPGPWYSLFTGAGLKYNEL

QGQRTRYIHKLHLKYGPTVRLSPSEASFTGPDAVREIYGSGGSGYEKTEF

YDLFTVYGRRTMFSTLDRESHARRKRVLADRYANTNVMKQPALSGIAERA

RRFAELCQGSVGGSLDIY (0)

TTLHSYAFDCVTHHLFHPLCTNSLQDKQDGDIMREVTFDDSLK (1)

NRLVKYYSPSLYHIVEPLLSFITQPRATPLADKFVLD

TSAQTHASPFTLLDRLHQRKNGGDLEQIDIAAECLDHMVAGIDTTGDALC

FLMYQLSLPSSATFQRKLQAELAANPDHDETPFDKLPYLDAIVQEGLRCF

PAIPMSLPRYVPSGGRHVDGYFMPEGTIVSCQAYSVNRINGDIFPHPETF

NPDRWLEVEGDATRRRCMFAFSHGGRGCVGKHLALAEMKLLLRDTYSRYS

TIPDSKMTDDAMAMSDQIISSRPAGQKCLMRFIPLVDNAT*

 

>CYP537A4 Nectria haematococca

e_gw1.9.142.1

Necha1/scaffold_9:176128-177897

79% to AACM01000224 Gibberella zeae, 79% to 537A2

gap in seq after TADVF, add cyan seq no good boundary at intron 3, GA not AG

57% to e_gw1.10.477.1

MLLLSPDFIAGAAALLLVIHVVVRLTSAMAKVPGPVVSNFTSLVLKWNELGANRTMYLHDLHKKYGPVVR

VAPNEVSFTSAAALKEIYGSGGSGYDKTEFYDLFQVYGKR (2)

TMFSTLNKGD (0)

HAKRRRMIGDRYANSNVMKTAPLAGIQVRSKKFIERCVSSPDGTADVF (0)

(?) xxxIALHAYACDCVTHQLFHPYGSNCLQNQDDEEMMHQVAADDSLK (1)

NRLVQHYSPTLHRVVSNVLASFAKPRSIPLADNFVIENAKEDGAADF

TVLNRLKEKNSVLDTIDMAAE CLDHMAAGIDTTGDVLCFLMWELSQPRSIKYQRLLREEFRNKPDAPFDE

LTLLDAVLNEGLRCFPAIPMSLPRYVPQGGRVIDGFSLPEKTVVSCQSFSVHRINDDVFPDPDKFYPERW

LETDGDADRRRLQWAFSSGGRGCIGKH (2)

LAMAEMKTLLRDVYSQYETEPDESMSTESMAMDDQLISSRPLGKRCLLKFVPVTEEEKNQ*

 

>CYP537A5 Nectria haematococca

e_gw1.10.477.1

Necha1/scaffold_10:12960-14643

MFLLSIPALSIAIAVCALFYVWKCVSSPLWAAPGPFLSRFTPFMLRWNEFNANRTLYIHSLHLKYGPIVR

IAPNEMSFSSYEAVKEIYGSKGSGYDKSTFYDLFTVFGRR (2)

TLFSTLDKTA (0)

HAKRKRLLADRYANSNVLKPASLNGIENRAREFARQCGDSTGSSVDVY (0)

IKLHAFACDGVTHHLFQPYGTDCLGNPGDREMMDQVNADDSLR (1)

SRLFMLYTPTLYKYSS

KILDCFFEPRATPLADNFVTKRSKLDDPEEFTLLSRLRSKLGDELAEGDIASECLDHMVAGIDTTGDVLC

FLIWELSQPRSAQFQDELRRELKGNPDMAFDKLPVLDSIVQEGLRCFPAIPMSLPRVVPKDGRVIDDIFV

PEDTIVSSQAYSVQRHHAHVFPEPDRFNPHRWMSNENDAEMRRHIFAFSYGGRGCIGKH (2)

LAYAEMKLLLREVYSQYQTVPDPSMTEESMRAHDQIISARPFGQKCLLRFVPTAA*

 

>CYP537A6 Fusarium oxysporum

87% to CYP537A4

FOXG_09472

MLILSPSFMLGAAALLVITHIIIRLTSATAKVPGPFVSNFTSLVLKWHELNANRTMYIHELHKRYGPVVRVAPNEVSFTS

IDALKEIYGSGGSGYDKTEFYDLFQVYGKRTMFSTLVKGDHAKRRRMIGDRYANSNVMKQAPLAGIQERSKRFVERCVDS

PDKTADVF (0)

LTKTALHAYACDCVTHQLFHPYGSNCLQNKDDEEMMHQVAADDSLQ (1)

NRLVQYYSPILHRLGSSILTSFAKPRLIPLADNFVIENAK

ENGAADFTVLNRLKEKDSVLDTIDMAAECLDHMAAGIDTTGDVLCFLM

WELSQPRSTKYQRLLREEFRSKPDAHFDELPMLDAVLNEGLRCFPAIPMSLPRYVPHGGRVIDGFSLPEKSVVSCQALSV

HRINDHVFPEPDKFYPERWLEAEGDADRRRHQWAFSSGGRGCIGKHLAMAEMKTLLRDVYSRYETEPDESMKSESMAMDD

QLISSRPLGKRCLLKFVPVKG*

 

>CYP537A6 Fusarium verticillioides

96% to CYP537A6 Fusarium oxysporum ortholog

FVEG_07101

MLGAAALLVVTHLIIRLTSATAKVPGPLISNFTSLVLKWHELNANRTVYIHELHKKYGTVVRIAPNEVSFTSIDALKEIY

GSGGSGYDKTEFYDLFQVYGKR (2)

TMFSTLVKGD (0)

HAKRRRMIGDRYANSNVMKQAPLAGIQERSKRFVERCVDSPDKTADVF (0)

LTKPALHAYACDCVTHQLFHPYGSNCLQNKDDEEMMHQVAADDSLQ (1)

NRLVQYYSPILHRLGSSILTSFAKPRLIPLADNFVIENAKEDGAADFTVLNRL

KEKDSVLDTIDMAAECLDHMAAGIDTTGDVLCFLMWELSLPRSIKYQRLLRQEFRSKPDA

NFDELPMLDAVLNEGLRCFPAIPMSLPRYVPHGGRVIDGFSLPQKTVVSCQALSVHRIND

HVFPEPDRFYPERWLEADGDADRRRHQWAFSSGGRGCIGKH (2)

LAMAEMKTLLRDVYSRYETEPDESMKCESMAMDDQLISSRPLGKRCLLK

FVPVKG*

 

 

>CYP537B1 Aspergillus nidulans AN8905.1 45% to 537A2 53 clan

revised 7/19/07

MLTFYLAASIIVTVLFMRRLCSPIAKLPGPWYATFTSWVLKYHEFTSNRR

LFIHDLHKKYGSTVRIAPNEISFASLEAIKEIYGSGGSGYDKTELYDLFRQFGIK

TMFSTLDKHS (0)

HSQRKRELADRYAMSNILREEHVSAIVDRARAVVSRCAASAESVD

VYVWLHCYALDGVTNFMFSPGGLHSLDSEHDFKIMEELTYHQSLQKNLLH

YYLPTVAAYFPPCLTPRRSPKTNEYVLKMCAQQLPSQHSLVAKLARKDSP

LNHTQVAAECKDHMAAGIDTTGDGLCFLMWKLSQPHNIRFQERLWEELRT

ARPDIPLDKLPYLDAVVKEGLRCAPPIPMSFPRYVPTGGRSIDGHFVPEK

TIVSCQPYTVHRLDENVFPEPDSFNPDRWMVEKGAVERNRLFFAFSTGGR

GCTGRNLALVEMKILLREVYSRFRTTVAKDMHGCMDIDDQIISSRPLGQT

CKLRFSET*

 

>CYP537B2  Aspergillus oryzae

          GenEMBL BAE66494.1

          67% to 537B1

          note: retains small intron due to lost phase 0 boundary

MWSLWLSSILLVGLYLFKRLSSPLAKVPGPWYTNLTSFCLKYHEFTATRRLFVHRLHKKYGPVVRLAPNE

VSFASLDAIREIYASGGSGYDKTEYYDLFRQYGIN (2)

TMFSTLEKQE

ISAYITIEAIATNDVQ

HSHRKRELADRYAMSNIVRDKHVSAIKERAQAFVSRCAAIEGSVNVY

SLLHCYALDGVTNFMFSPGGLKSLDNTKDYEMMEELTYHQSLQKNLLYYYLPRLAPYFPSCLHPRPAPR

ANAYVLQMAGQQHPEAHSLVARLTRKGSPLSHMQVAAECKDHMAAGIDTTGDALCFLMWELSQPQNLQFQ

DRLHKELLSTSDDTPLDKMSYLDAVIKEALRCAPPIPMSIPRYVPSGGRTIDGYFIPENTIVSCQPYTVH

RFNEEVFPEPDRFNPERWLEEKGFNDRNRLFFAFGTGGRGCTGKNLAMVEMKILLRELYSRFRSSVAPDM

TASMDLDDQIISARPKDQICKLNFAVRGDDVDTA

 

>CYP537B2  Aspergillus flavus

98% to CYP537B2  Aspergillus oryzae

AFL2G_11813

MWSLWLSSILLVGLYLFKRLSSPLAKVPGPWYTNLTSFCLKYHEFTATRRLFVHRLHKKYGPVARLAPNEVSFASLDAIR

EIYASGGSGYDKTEYYDLFRQYGIK (2)

TMFSTLEKQE (0)

HSHRKRELADRYAMSNIVRDKHVSAIKERAQAFVSRCAAIEGSVNVY (0)

SLLHCYALDGVTNFMFSPGGLKSLDNTKDYQMMEELTYHQSL

QKNLLYYYLPRLAPYFPSCLHPRPAPRANAYVLQMAGQQHPEEHSLVARLTRKGSPLSHMQVAAECKDHMAAGIDTTGDA

LCFLMWELSQPQNLQFQDRLHKELLSTSDDTPLDKMPYLDAVIKEALRCAPPIPMSFPRYVPSGGRTIDGYFIPENTIVS

CQPYTVHRFNEEVFPEPDRFNPERWLEEKGFNDRNRLFFAFGTGGRGCTGKNLAMVEMKILLRELYSRFRSSVAPDMTAS

MDLDDQIISARPKDQICKLNFAVRGEDVDTA

 

>CYP537B2 Neosartorya fischeri

100% to CYP537B2  Aspergillus flavus

seq runs off the contig end

LADRYAMSNIVRDKHVSAIKERAQAFVSRCAAIEGSVNVY (0)

SLLHCYALDGVTNFMFSPGGLKSLDNTKDYQMMEELTYHQSLQ (1)

KNLLYYYLPRLAPYFPSCLHPRPAPRANAYVLQMAGQQHPEEHSLVARLTRKGSPLSHMQVAAECKDHMAAGIDTTGDA

LCFLMWELSQPQNLQFQDRLHKELLSTSDDTPLDKMPYLDAVIKEALRCAPPIPMSFPRYVPSGGRTIDGYFIPENTIVS

CQPYTVHRFNEEVFPEPDRFNPERWLEEKGFNDRNRLFFAFGTGGRGCTGK (2)

NLAMVEMKILLRELYSRFRSSVAPDMTASMDLDDQIISARPKDQICKLNFAVRGEDVDTA

 

>CYP537B3 Aspergillus niger

e_gw1.14.367.1|Aspni1

71% to CYP537B1

MLGLWCAAILILVFLYQRLSSPISKLPGPWYTKLTSLVIKYHEFSASRRVFIHQLHKRYGTIVRIAPNEVSFASLDAIRE

IYASGGSGYDKTEFYDLFRQ

TMFSTLDKQT (0)

HSERKRQLADRYAMSNILREHHISAIRHSAKAFVSKCAAVAKSVDVYVYL

HCYALDCVTHFMFSPSGLNSLTDDNDFELMEELTYHNSLQKNLLPYYLPRLAPYFPSWLLPRRAPKANMYVHRVTAETNP

DEHSLLARLLRKDASLSKSEAAAECKDHMAAGIDTTGDGLCFLMWELSQPHNFPFQDRLYEELRSAPEDTPIDRLPYLDS

VIKEALRCAPPIPMSFPRYVPRGGRTIDGVFIPEGNIVSCQPYTVHRLDTKVFPEPDMFNPDRWTKDEGFNERNRLFFAF

STGGRGCTGRNLATVEMKILLQEVYSRFRTTVAPDMTSSMEIDDQIIASRPKGQKCRLIFTPREI*

 

>CYP537B4 Aspergillus terreus

73% to CYP537B2 Aspergillus flavus

ATEG_07898.1

MLSLWCLGIVGLGVFLFRSLSSPVAKVPGPWYTLFTGLVIKYYEFTATRRVFIHDLHKRYGPVVRIAPNEVSFASLEAIR

EIYASGGSGYDKTEYYDLFRQFNIK (2)

TMFSTLDKHT (0)

HSRRKRELADRYAMTNILREIHVSAIRERAEAFVAKCAAIAKSIDVYVYLHCYALDG

VTNFMFSPGGLRSLDRAEDFAIMEELTYHQSLQKNLVHYYLPSIVPYLPSFLIPRRSPLTNDYVMTMTNQAQPEPHSLIS

RLARPDSPLSSLEAAAECKDHMAAGIDTTGDGLCFLIWELSQPQNMRIQDRLYEELQSVPSDTPLDKLPYLDAVIKEGLR

CFPPIPMSFPRYVPAGGRTIDGYFLPQDTIVSCQPYTVHRLHEDIFPDPDRFYPERWLEEKGAAERNRLFFAFGTGGRGC

TGRNLAMVEMRVLLQAVYSRFRTTVASDMTGCMDIDDQIISSRPKDQTCRLDFARRE

 

>CYP537C1 Nectria haematococca

e_gw1.38.28.1

Necha1/scaffold_38:43917-45683

43% to 537A3 Magnaporthe g. 42% to 537B1

add cyan exon

MIAELALQSKTLGLLLVAFGSVLVIAIVFYHVAFSPMRAIPGPWYLRLTSLFVKYHEFHGTRRLWIHDLH

LKYGPTVLLAPNEVSFASASALKQIYSSGGTGLPKDHMYSLFKAQGHT (2)

NLFTALDNKE (0)

HREKRKQLSERYSNTSILKSPILDVIKERAEAFATECRKTASADVY(0)

FLLHGYALDCVSAMLFHPHGTKSIEPGDQREMVHLLSYHDSRK (1)

TLVLERYWPLLQRIYKFLSHKPTKGGKPIDDYVWS

SMSKTDYSEFSLMARLLHDKKMPVDAIASECYDHITAGIDTTGDTLCFLLWELSRPSQQERVKRLREELV

VGKSSGQPLDTLPYLNAMLQEALRLWAPGMQSLPRNVPAGGREIDGHFVPAGTVVSCQSYTTHRYDDDVF

QDADAFVPERWLNPDGDSERGRLFFAFGSGARTCIGRH (2)

LAMAEMRALVHAIYSKMRTVPAQDMVASMEFNDQIMTTRPEGLCCKITFEPLTD*

 

>CYP537D1 Nectria haematococca

e_gw1.23.292.1

Necha1/scaffold_23:445038-446732

51% to e_gw1.9.142.1, 48% to 537A2 47% to e_gw1.10.477.1

43% to CYP537B1 AN8905.1 in CYP53 clan

add cyan region

MALLSLPLLAGATLLLFVIALVRRLFSPLSRFPGPKLGLLTPWQLRYHELQGKRTQYIHQMHQQYGNVVR

VAPNEVVFSSLEAMKEIYLSKGSGYDKPSFYDLFSQFGLR (2)

TMFSTREKGP (0)

HSKRRRIIADRYANSNVIRDSALHGIQERSQNFIKRCSEAPRQELDVY (0)

LFLHCYAFDCVTHHLFHPHGSDSILKQSD EETLVEAVFDNSLA (1)

RRLFIHYNPTIGSLMGKLGVFGKS

RDIPRTRQLILDGATGGPVADFTLVSRMREDKFGMGPMVIASECMDHTLAGIETTGDALCFLMWQISQPG

YEIIQERLRDEFLANEGVSFEQLPYLEAVVKEGLRVFPSIPMSLPRCVPPEGASVDGHWLPGGTLVSCQP

YSMHRMDEVAFPEPDSFKPERWLEEKGLAERNRLFFTFSNGGRACIGRH (2)

LAVAEMKTLMRDIYSTFKTTPAEGMAADMSMDDQIFTSRPKDQKCILKFTRWEEELV*

 

>CYP537D2 Fusarium oxysporum

78% to CYP537D1 Nectria haematococca

FOXG_09731

MATLSLAHLAGAAVLIFIGVLIVRLRSPLASLPGPRLGLLTPWQLRYHELRGKRTQYVHRMHQKYGNAVRVAPNEVVFSS

LDAMKEIYLSKGSGFDKTSFYNLFSQFGLR (2)

TMFSTLQKGP (0)

HSQRRRVIADRYANTNVNREASLHGIQERSQNFIARCKDAPKQELDVY (0)

MFLHCYAFDCVTHHLFHPHGSDSILQASDEETLHEAVFDNSLVSK (2)

LLNYYHPTLGSVAGKLGLFGKSRGIPRATELVLNGVKKGGVADFTLVSRMQEEKFGMGTFDIASECMDHMLAGIE

TTGDALCFLMWQISQPGYTFIQERLRDEFRANPDVPSDQLEYLEAVVKEGLRVFPSIPMSLPRCVPEGGATVDGHWLPGG

TIVSCQPYSMHRMDEGVFPRPDSFEPQRWLEEKGSAERNRLFFVFSNGGRACIGRH (2)

LAIVEMKTLLRDIYSRYRTAPADGMSFDMSMDDQIFTSRPRDQKCNLKFVEWVEVAGC*

 

>CYP537D2 Fusarium verticillioides

94% to CYP537D2 Fusarium oxysporum = ortholog

FVEG_08745

MAVLSISLFAGAAALIFIGVLIVRLRSPLASLPGPRLGLLTPWQLRYHELRGKRTQYVHQ

MHQKYGNAVRVAPNEVVFSSLDAMKEIYLSKGSGFDKTSFYNLFSQFGLRTMFSMLLKGP (0)

HSQRRRVIADRYANTNVNREDSLHGIQERSRNFITRCRDAPKQELDVY (0)

MFLHCYAFDCVTHHLFHPHGSDSILQASDEETLHEAVFDNSLVRK (2)

LLNYYHPTLGSVTGKLGLFGKSRGIPRATELVLNGVKKGGVA

DFTLVSRMQEEKFGMGIFDIASECMDHMLAGIETTGDALCFLMWQ

ISQPGYTFIQERLRDEFRANPDVPFDQLEYLEAVVKEGLRVFPSIPMSLPRCVPEGGATVDGHWIP

GGTIVSCQPYSMHRMDEEVFPRPDSFEPQRW

LEEKGSAERNRLFFAFSNGGRACIGRH (2)

LAIVEMKTLLRDIYSRYRTAPADGMTCDMSMDDQIFTSRPKDQKCIMKFIEWD*

 

>CYP538A1 N. crassa AABX01000024.1 cont3.89 NCU02031.1 (version3)

27319 MLTPTTLLLLSPLLYLLTLLFRTALYRLRARSLGCSPVAVYPHK

      DPILGLDLFRESLRAMNTHALLPLWDARFKRYGNTHYTLTLGKW

      VLMTNEPENVKVILGTKMAEWPIDGPRLHASVAVLGKKSIFTTN

      GAQW (2) 26912

26807 REARGMIRPSFVRDQVADLHCFAKHVGNFLNAIPKDGSTFDMQE

      LLLSMTMDSSTDFLLGCSTNSLLQPSPEAKQFLEDFEYTSREAA

      KKSRLGTLLNWLPNGEFQATVTRVREYVRAYIRKSQAEKTDKKE

      RDYVFLHEILKSGADEEHAIDQVLSVIIAGRDTTAAAMTACFYY

      LARNPEAVKKLRKEIFDMEDEMPTWEQLKNMKYLNMIIKEG (1) 26156

26088 LRLFPPASTNSRAPIKDTVLPRGGGPDGKQPILVPKGQVVRWSL

      YSLHRRKDIWGEDAHEFRPERWDENLRVG (2) 25870

25787 WEYIPFSGGPRICLGQQFALTQIEYALFKFFRAFKSIEPRDDNG

      PLLLRTNLTVTFAKGCLVSATPDSN* 25578

 

>CYP538A2 Magnaporthe grisea MG02792.4  54% to CYP538A1 AACU01001559 cont2.569

MNRIALWTVASFLLVPFTAHACLQTWHILAGTSSPTGKWRSRATYILSGL

LLKVQYDLFVKLTLELRARRLGCLPAKLYPHKDPVLGLDYFFKALRAAGA

GQFFPFARENLKTIGHTHYAMALGTWVLHTDEPENIKSILATDFDSWEIA

GPRLLPVLDVLGPKSIFTSNGEGWHQARSMLRPSFVRDQVADLACFDRHI

SHLLKKIRSSCQEGMVAFDLQKLFFLMTMDSSTDFLLGRSTDLLVKASPE

AEDFLRAFDYVLFQGAKMARLGPIMMKLPHRKFDAEVQTVRSFVRKYVSK

AMAEKGYQKERSYLFLHELVASGASVEYITDQVLSIILAGRDTTAAALAA

AFYFLSKSPDIVDKLRQEMLEMGEENPDWETLRGMKYLQNVIKEG

LRLAPPVATNSRTSCKETTLPRGGGPDGKSP

VLIPKGTSCRWSNYSAYRRADIYGPDADEFRPERWEDLRVAWEYTPFSGG

PRICIGQQFALTQMSLAIFRILQNFDKIKSKNEGPMGMRAGVTVSLADPG

CLVSMTPIA*

 

>CYP538A3 Nectria haematococca

fgenesh1_pg.scaffold_1000354

Necha1/scaffold_1:1132876-1134514

49% to N. crassa 538A1

MPHPLWVVTLLTPAILYLRSKYYWLHWMRVHDTQPAPVYPHRDRLLGIDWMLEMSKAIRSHSILQLWDGL

FSSLGNTFWTKNVGAWIIMTNEPENVKALLSGQFETWQIAGVRQAAMVLALGPNAIFSVNGHAWHDARTM

MRPSFVRNQIADLECTDRHVEAFLDRIPRDGQAVDLQELLYMFTMDISTDFM (2)

FGYSTNILSDPSSEALDF

TRSFDYALLSAAQRARLGWIVLLMPDRKLNEAAATCRRFIDRYVKEALASSREKERPYVFMNEMFASGAS

QEYVRDQLLAMILGGRDTSASTMSSLFWILARRPDVVEKLRAEIAGLDGRKPTWEELKELKYLNMVLKE (1)

ALRLWAPVSTNSREAVKDTVLPKGGGPDGQSPLFVPKGTACRWSSYSLHRRKDIYGDDAEEFKPERWESLRAK (2)

WEYIPFSGGPRICIGQQFALTQMSYLITRLFQTFESMEAADDKPMLQSVSTTIALVNGCWVRLTPVGAS*

 

>CYP539A1 N. crassa AABX01000502.1 cont3.571 NCU09115.1 (version3)

3508 MGLVEDVAGNLSLKNTPIIFGGVLLGLIVFRYLQVVWQNLRIARM

     GLRPPKIRDSGLF (1) 3681

3740 GIKFIKTNVKMAAEHKNLQFWQQMFRSIGGYTGEVRLVGHRIIFT

     SEPENMKAILATQFEDYGKGEGFHQEWKDFLGDSIFTTDGDLWHA

     SRQLIRPQFIKNRVSDLQCFENHMQMLFRTIANGGALNGEDQMVD

     MEAGNGKPVDISDLFFRYTLDAATDFLLGKDIKSLS (2) 4252

4308 TPVQPFADAFQEVQRVQIVIARAGPLNRFVPKKTFWEGLKVIDET

     INFYIDRALRLDEEELASKSKGDEGYTFLHALAGFTKNRQVLHDQ

     LMAVLLAGRDTTACTLS WAIYELARHPEAVAKLRAEILSVVGPDR

     APTYDDLKSMKYLQNVMNETLRLYPVVPFN (2) 4782

4867 VRMALKDTTLPRGGGPDGSQPIVILKDTPVGYSPLAMQRRPDLYP

     PVSEKFPDVEMFSPDRWFHWQPKPWQYIPFNGGPRICIGQQFALT

     EMGYVLTRLFQRYD RVVSYMDEIDGGKPRMKTDIVLMPGDGVKVA

     FFEAKRE* 5295

 

>CYP539A2 Magnaporthe grisea MG09920.4  64% to CYP539A1 AACU01001746 cont2.1896

MGLVEVVLEHVSVKAAVLWIVGSWVLYLVVKNVLEERKINKLGARAARMP

SRLPLSLDNIIRAVRANIQNRNLQMWRSHMSHSITYTVEGRLLFQRIILT

ADPENIKAILATQFTDYGKGEPFHQEWKPFLGDSIFATDGEKWHTSRQML

RPQFVKDRVSDLECFESHIKTLFKAMANGGALEGEHQHVELSAINGKRFE

ITDLFFRFSLDVATDFLLGYDVKSLSTPHAEFAEAFNEVQHVQSTITRSG

PAQALVPKGSYYRGLKVMDNFINPFIDRALRLSPDELASKTKSDHGYTFL

HELASFTRDRKVLRDQIVAVLLAGRDTTAATLSWTIYELGRHPECVKRLR

QEIAEVVGFGRTPTYADLKSMKYLQNVMHETLRLYPAVPFNVRLALHDTT

LPRGGGPDGTLPVPVLKDTPIGYSTLLMQRRRDLYPPVSENFADPDVFSP

ERWFHWQPRPWQYVPFNGGPRICIGQQFALTEMGYVLVRMFQQYERVVSY

MDEIDGGNPTIKADIVMQPGDGVIVAFWEGDNEKI*

 

>CYP539A3 Fusarium graminearum FG01284.1 AACM01000065 FGcontig1.65_scaffold1

MGVVEALLEQVTLKTTLVILFVSYCIYSIICRIDEHRRIRRLGHYGPHMK

TYAPWGLGIVARFVVSTVKHQNLATWRDDMFGAENSWTVETRLLGVRTIF

TADPANLKAILATQFVDYGKGKPFHAEWKDFLGDSIFTTDGAPWHASRQL

IRPQFTRDRVSDLHCFEAHMQTLFKAIANRGPLHGEDQPVPTEGLDGKVL

DISDLFFRYTLDVATEFLLGWDVKSLTTPRQEFAEAFNDVQRIQNIVART

GKLRHIIPRYKFWKGLKTVNHFINFYIERALRLSPEELNSKTKDDHSYTF

LHALAGFTRDRKVLRDQIIAVLLAGRDTTAATLSWTLYELGRYPNAVKRL

RSEILSTLGSERTPTYDDLKSMSYLKAVLNETLRLYPAVPFNVRLALKDT

TLPRGGGPDGSEPLPVLKDSPVGYSTLVMQRRSDLYPPISDTFADPQIFS

PERWAHWHPKPHDYIPFNAGPRICIGQQFALTEMSYVLCRLFQKYERVES

RMKDIDGGEPLLKADIVLSPGQGVNVAFWEAKK*

 

>CYP539A4P Aspergillus nidulans AN5478.1 PSEUDOGENE

LSAKGIDCAVRAVYQLLNYNFVEWTQNVLEENGRTVELHLAGARLVLTDDCENVKAIMFS

GKGKFTHEVFASVFGDSVFGS

LNRCGTRSPPSAKALKDLVFLKYVIRETLRLY

AMQDPVAITITSALFELVRHPEIAQRLREEVVRV

VVGFNIREAQRDTTIPIGGGPNGDLLITILKGQHVAYSVIGLQRRPDIVGPDADNWRPDGY

310339 MGTFHWAPQTWEFIPFNHGPRICLGRVFGYFQMEYTLCRIFQHFERVE

LHEPRVQRIKVELNTKMAYPVNCSFHRAVY*

 

>CYP539A5 Nectria haematococca

fgenesh1_pg.scaffold_2000110

Necha1/scaffold_2:391800-393499

78% to 539A3

This gene model seems correct

MGLVEAFLEQASFKAVFLIAAAFYTIYYIGCRIDEHIRIRRLGRYGPYLRTYAPW (1)

GLDRLVKFVYATAKH

RNMEVWRDTIFGAHNSWTVESRFLGVRTIFTADPANVKAILATQFGDYGKGEPFHREWSDFLGDSIFTTD

GAKWHASRHLIRPQFTRDRVSDLHCFESHVQTLFKAIANGGPLLGEAQVVDMDSVDGKVLNISDLFFRFT

LDVTTEFLLGSDIKSLT (2)

NPRQEFAEAFNEIQHIQNIVARTGKLRHFYPKPRFWHCLNVVDNFINKYIDRA

LRLSPEELASNSKDDHGYTFLHALAGFTRDRKVLRDQIIAVLLAGRDTTASTLSWALYELGRYPHAVAKL

RAEILSTVGPDETPTYEHLKNMPYLKSILNETLRLYPSVPFNVRIALKDTTLPRGGGPDGTEPLPVLKDS

PVAYSTLVMQRRSDLYPPVSEKFADPQIFSPERWAHWFPKPHDYIPFNAGPRICIGQQFALTEMSYTLCR

MFQRYERAVSYMKEIDGGKPLLKADIVLSPGQGVKVAFWEATRA*

 

>CYP539A6 Mgr018 Mycosphaerella graminicola

54% to CYP539A1  N. crassa

MLVALIDRVWANTVLYLIGVATAWSIRQYSSPLISSGLLVGATVVYLMNFIGHVEQERKIENLGGHTAQLRTFSPWNISVLFQGITYARKSMNHIFWGLIFTRAGAGNHSHTAEVQAAGQRIIFTRDEENIKAILATQFQDYGKGPQFRKDWGHFLGLSIFTTDGEIWHNSRTLLRPQFIKDRVSDLHTFEHHIQELLPMLAGDHDGATVRLDDLLFRYTLDAATGFLFGSGVGSLKNGETEFALAFAEAQRVQSIIARAGPMNGLVPRKSFNEAVKILNEFTGRFIDE (0?)

ALALPQHELEKKTKSDEGFTFLHALAGYTRDRTILRDQLVAVLLAGRDTTACTLSWLFYELSTHPEIVKKLRQEILSVVGPNEEPTYDHLKAMRYLTHTMNETLRLYPVVPYNVRVALKDTTLPHGGGPDGLQPIGITEGTPIGYSTFVMQRREDIYPSAKEGFPDPKQFVPERWEGWTPKAWTYIPFNGGPRICIGQQFALTEMAYTVVRILQTYDTIISKNEKFPGTKADIVLQPADGVFVAFKKASRS

 

>CYP539A7 Fusarium oxysporum

88% to CYP539A3

FOXG_00101

MGVVEALLEQISVKTTFIVLFVAYGVYSIICRIDEHRRIRRLGRYGPQLKTYAPWSLDLVARFVRSTVKHNNLASWRDGI

FGPLNSWTAEARLLGVRTIFTADPANLKAILATQFVDYGKGQPFHAEWKDFLGDSIFTTDGPSWHASRQLIRPQFTRDRV

SDLHCFEAHMQTLFKAIANRGPLRGEGQLVDMNNTDGKVLDISDLFFRYTLDVATEFLLGWDVKSLTTPKQEFAEAFNEV

QHIQNIIARVGKLRHIIPKYRFWRALNTVNHFINFYIERALRLSPEELASKTKDDHSYTFLHALAGFTRDRTVLRDQIIA

VLLAGRDTTAATLSWTLYELGRYPNAVKKLRAEIISTLGTERTPTYEDLKSMSYLKAVLNETLRLYPAVPFNVRLALKDT

TLPRGGGPDGSEPLPVLKDSPVAYSTLVMQRRSDLYPPTSDTFADPQIFSPDRWAHWHPKPHDYIPFNAGPRICIGQQFA

LTEMSYVLVRLFQKFDRVESQMKDIDGGEPLLKADIVLSPGQGVKVAFWEAQN*

 

>CYP539A7 Fusarium verticillioides

97% to CYP539A7 Fusarium oxysporum = ortholog

FVEG_01415

MGVVEALLEQISVKTAFIVLFIAYVVHSIICRIGEHRRIRRLGRYGPQLKTYAP

WSLDLVVRFVRSTVKHNNLASWRDGIFGPLNSWTAEARLLGVRTIF

TADPANLKAILATQFVDYGKGQPFHAEWKDFLGDSIFTTDGPSWHASRQLI

RPQFTRDRVSDLHCFEAHMQTLFKAIANRGPLQGEGQFVDMNNTDGKVL

DISDLFFRYTLDVATEFLLGWDVKSLTTPKQEFAEAFNEVQHIQNIIARVG

KLRHIIPKYRFWRGLNTVNHFINFYIERALRLSPEELASKTKDDHSYTF

LHALAGFTRDRTVLRDQIIAVLLAGRDTTAATLSWTLYELGRYPNAVKRL

RAEIISTLGTERTPTYEDLKSMSYLKAVLNETLRLYPAVPFNVRLALKDT

TLPRGGGPDGSEPLPVLKDSPVAYSTLVMQRRSDLYPPISDTFADPQIFS

PERWAHWHPKPHDYIPFNAGPRICIGQQFALTEMSYVLVRLFQKFDRVES

QMKDIDGGEPLLKADIVLSPGQGVKVAFWEAQD*

 

>CYP539A8P Aspergillus terreus

note: best matches are CYP539A seqs but

no I-helix motif LAGRDTT is found suggesting a probable pseudogene

C-term half is 48% to CYP539A2

CYP539J1 is 3 kb upstream

ATEG_08168.1

MRCVYELATHGFHEYVRDILATKPGRTIEFHILDKRMIVTDNPENIKEMMSVQFDTFGKGDLTHHIFRNCFRGSIFGSEG

AEWAAHRAQLKPHVGTMRPSDPEKLEKHFLQMLKKNIPDD

GSPVEVYDMLDM

MLLETVIDIFVDPSKDPEYKHIDPRPFV

NGVNALLKINTFRVLLGNVSRLVSDTLVARASTQALHEYLGSHVRWIRKLQSSNYGEKP

KKEWTLMETFASEPLPDSDPVGMI

WVIYELGRRPEIVDKLREEIIATIGDDINTLPTDAQLRSMKYLQNIIKEAMRMYHPF (0)

LTFAVGFNIRCPNKDTVLPTGGGPNGDQPLPILRETFI (1)

VVNIMGLHRRADIVGA

DTDVFRPERWDTFKPGPWEYMPFHRGPRNCLGMAFGQYAMAYLIVRLYQLYD

ILPADNIVQRIKVEMNTKVSHP VNMRFYPRQSVSSPRLQKDEVLL*

 

>CYP539A9 Mycosphaerella fijiensis

64% to CYP539A6 Mycosphaerella graminicola

estExt_Genewise1.C_13960

MILHILDRLGWLTLLYSSITYCTYQLRNLIPEKLCYALLLLLTIVYLKKVIHHLDQESRIRRLGKRAPSVRTPYPFNLGV

MFQALYYFSQHRNHEFWWNLFKKGNPNRPYTVEALTLGGRLVFTADEENIKAILATQFSDYGKGPQFRKEWKDFLGLSIF

TTDKELWHNSRQLLRPQFIKDRVSDLQKFEHHVQILLPLMAGSHNGATVRLDDFMYRFTLDAATDFLFGTSVGSLLNGEA

EFAQAFAEVQGVQAIIARSGPLASLYPKKKFHEALSVMNKFTDRYIDQALQLPKEELEKKTKSDEGYTFLHAIATYTRDR

QVLRDQLVAVLLAGRDTTAVTLSWLFYELSLKPEIVKKLRQEIVSHVGLHREPTYDDLKSMRYLQHTLNETLRLYPVVPY

NVRVALEDTTLPHGGGPDGMEPVGIAKDTPIGYSTFVMQRRADIYPPESDKFPHYLKFAPERWDHWTPKSWTHIPFNGGP

RICIGQQFALTELAYTVTRILQTFERVECRMDEYPGTKTDIVLQPAKGVYVAFVKAGSV*

 

 

>CYP539B1 Magnaporthe grisea MG06973.4  45% to CYP539A1 AACU01000620 cont2.1289

MFGGLVEVRPLPLAGFLLGVYVAAWFIQAALMDYRIAKRGGGGVKAPRVA

SNMITAGIWFFWAGWRQMRNDLLSLYNSVYDKHGTPESPNCVEITLTTPG

VRFILTREPEHVKTILTSKFSDFGKGYHFHKIWSPFLGDSIFTTDGQLWH

DSRSLIRPMFIKEKVRDLDIFDRWTNEMINLLPPPGQSVDIANLLYRMTL

DVTTDFLLGESVNALRDEHNEFAYYFNEIQRIQMMLTILVPAAPFLPRHR

YYAGIKYIEKFMAPFIEQALALPPDELEKLSKTDKDFTFLHNIARYSRDR

KVIRDQIMAVLLAGRDTTAATLSWTIYELCNKPEVVKRLRQEILRVVGPF

GNKPTYENLKELKYLTHIINETLRIYPAVPYNIRAALEDSSLPSPDGKPS

IAVLKGDIVVYSALSMQRRKDIYPEVSETFPDPAVYCPERWEHWTPKPWT

YVPFNGGPRICVGQNFAMTEMAYVLVLIFQKFDRIEYCGDWDAQYHKAEI

VGAPGHGVPVTLYPAVDDQVKE*

 

>CYP539B2 Aspergillus nidulans AN4858.1 53% to 539B1 49% to 539A1 52 clan

revised 7/18/07

MSQMDPKIYLLLFLSPVLAFSGLILIYILTRPIRLFLYDRRFYLKGGIHAPMIPRDIIS (1)

VTRFFFAVVKAQNEHRLYEFFKKSLEHGD

PASPNCVESNIFGSFRVIQTREPEHLKAVLTGKFADFGKGELFHKLWIPF

LGDSIFTTDGKEWQGSRNLIRPMFIKDRISDLDIFERKTQT

MLSLYSPCG

EPTDVVDLFYRMTLDAITEFLLGKGINSLENPQADFALAFADVQRIQTLL

TMLGPAQYLYPRGRYNQGLKVINDFVWPFVHDTLGFQADDLKSSDKSFTF

LHALANYTRSPKTIRDQVVSILLAGRDTTAATLSWAFYELSHYPEAYSKL

RAEILDKVGPTRAPTYDDLKNMPYLRHTINEVLRLYPAVPYNIRFALTDT

TLPIGGGVNGDLPITILKGDAVAYSTYAMQRRADLYPPVSEKFADPAIFS

PERWEVWSPKPWHYVPFNGGPRICIGQNFALAEMGYTIVRILQRYERIEY

VGDWERQFHKSEIVGTPGMGVKLRLYEARKP*

 

>CYP539C1 Magnaporthe grisea MG08234.a  42% to 539A1 top half AACU01000307 cont2.1540

2 P450s fused

MIQKYGGHAVKFSFSNSPLDNIFGLRFAWEFYRHGRRDEYLKFTHRSFAQ

SGAGRANPYTYEVNLGGVRTIWTADPENIKAMLGSQFEDYGRGKAFQSRW

HDLLGTGIFNADGREWHDSRHRLRPLFNRQRVSNLDSFERQIQIMINRHL

GGGRTVDLKDVISRFALDAIGDYAMGFQVNALADRKNDFLDALERIKTIQ

NIKECAGPLSWLVPTPGFKRDLKVLDDFMEPLIEHAASLPQSVLDEMEKT

DHGWTYVKACASVSRDRHFLKYELMSVILAGRDTVSGTLVWTFLELVKRP

DLMADLRREIENTVGIGRDAPRPTHDDLRSMRLVKNTLNETLRLYPTVSL

NLRTALRDTSLPRGNDHDGNKPVGLAEGTPVIFSSHILQLSPETYEQSPP

GTSPPHVFDPYRWEEWKPKPWTYIPFGGGPRICIGQEFALAEMAFLLVRL

LQNYSRLEMRCEMRGNSDEGWERPNGSASIVEQFM

 

>CYP539D1 Aspergillus nidulans AN3917.1  52 clan 53% to 539A1 MIEELVSKTTPGQAVVYLLGAFFLACLARKIQVQWQLSRLGSRAPKIPFR

LPYGLLIPSISRNSHADRRLSSAMDFIYKSMQANKSDQDLEYFTDVMRNA

KGAPLAEDKKTVELDAGISSRIIFTRDPENIKAILTGQFSDYGKGESFHR

DWREFLGDSIFVTDGEQWSASRHLIRPMFVRDRLVDTEIFEKHVQHLIPL

LAGNNESKIVD

VTSLFFRYTLDAATNYLLGQGTDSLQNPETEFAEAFRYV

QRRQAQYFRLGVFKFVLSRTEFRRQLKVMDDFIKPYIDRVLAMSPAELDQ

KLSKSDTFLHALARFTRDRRTLRDQLVAILLAGRDTTAATLSFCLFELAR

NPEVTAKLREEIASRLGLGASAQKPSYTDLKEMKYLNAVLNESMRLYPVV

PFNVRFSLKDTTLPRGGGPDGLAPVAVRAGTRVVYSTMIMQRSPENYDPP

SAPNYFDPAQWNPERWLSGWQPKPWQFIPFNGGPRICIGQQFAMIEMGYT

VVRILQAFERIQAVPEAGKSVVEDPRLRFEVTLTPASELNCVFVRDGEGR

EDEKISR*

 

>CYP539D2  Aspergillus fumigatus Af293

          GenEMBL XP_750735.1 also EAL88697.1

          71% to 539D1

MIEELLAQIHSGWAALYVLGALFFACLIRKAQVSMEISKLGYRAPRIRFYLPWAIDFLYEASKANEDGTD

HVFLHQAILNAKGATQLDCVKTAELNGGITRRVILTKDPENIKAILTGQFADYGKGEEFHEQWKDFLGDS

IFATDGELWSRSRHLIRPMFVRDRIVDTEIFEKHVQNLIPLLEGSNSPSGSKAVDVGSLFFRFTLDAATD

YLLGQGTNSLHNPETRFAEAFGYVKHRQSEIFRLGDGISMFSFIVAKRKFRRELKVMDDFFQPYIKRALS

LTPSELDQKISKRETFLDALARFTRDPRVLRDQIVAVLLAGRDTTAAVLTFCIFELARNPDVVAKLREEI

SARLGLGPSAQKSSYNDLKEMKYLNAVLNETMRLYPPVPFNVRYSLRDTTLPRGGGPDGLSPVGVRANTR

VIYSTMIMQRSAENYDPPGSPNYFDPEKWLPDRWLSGWQPKPWQYIPFNGGPRICLGQQFATIEMGYTVV

RILQAFERIHAMPANGKERVEDPVLKFEVTLSPGSELNCVFVREGEDTLPLKDG

 

>CYP539D3 Aspergillus niger

e_gw1.4.261.1|Aspni1

67% to CYP539D1

MLEYLVGQMSPMKASACLVGIFLIACLVRKFQVSLEVSRLGLRAPRIRFRLPYALDNVYGYVMANSRGEDLTHWQNLMET

AHGASAIDHVQTAELDFGISNRAIVTKDPENIRALLTGQFADYGKGEPFHHDWKEFLGDSIFVTDGELWSRSRQLIRPMF

TRDRIVDTEIFEHHVQHLIPLLAGTKSNKVVDVTPLFLRYTLDAATAYLFGDGTNSLQNPATSFAEAFSYVQHRQSEIFC

YGYTSTNNRSISPFNPILSRTKFRQELKKMNDFMAPFIQRALSLSPEELDTSLSKQETFIHALARFTRDPKVIRDQLTAI

LLAGRDTTATTLAFCFFELARNPSVVAVLRSEIASRLGLGPSGRKPTYTDLKEMKYLTAVLNETNRLYPVVPFNVRYALK

DTTLPRGGGADGNGPVGIRANTRMIYSTMMMQRDPAHYPPPGSKNYFDPGKWVPERWTGDWVPKPWHFIPFNGGPRICLG

QQFAMIEMGYTVVRILQVYESLRAMPPAGKDKVEDPMLRFKITLNPGSELNCVFE*

 

>CYP539D4 Uncinocarpus reesii

57% to CYP539D3

UREG_01046.1

MLEELASRFSPAQAALVAFGLFAVFLFILDCRIDWRIRKLGAQSPRIKSYLPLGFDFIYRVMKAGSQSKDLEFWTELAKE

LPSHGPGPVGTYEVKFRCYGRSVFTTDPDIIKTVLTGQFADYGKGKRFHEEWRDFLGDSIFATDGELWSKSRQLLRPMFA

RDRIVDTEIFEKHIHKLIPLLSGDLNQDGSKTVDVGPLFFRFTLDAATDYLLGHSVDSLDNPKTTFAEAFQYVLHRQSVL

FRAGPISPLMSRKLFHENLKKMDDFMQPFIEQVRALTPEELDHKLSKKDTFLHALARFTRDPTVLRDQLVAVLLAGRDTT

AATLSFCLFELSRHPAVVQKIRHEIASVCGTRKPTYSELKEMKYLNAVLNETMRLYPVVPFNVRHSLVDTTLPRGGGPDG

LSPIGVPADTRIIYSTMAMQRRRDLYPAPPLPGEKPSQPYFDPLLFHPERWTSGWQPKPWHFIPFNGGPRICLGQQFATI

EMAYTITRILQHFTGIVGVGTPPPGTDPKFKFDVTLSPGQRLDCVFVKEGN

 

>CYP539D5 Coccidioides immitis

85% to CYP539D4 Uncinocarpus reesii

CIMG_01103.2

MLEELVTAVSPGQAVLIAFGLFATFLFILDLKIDWRIQKLGPQAPRVRTYLPLGADFIYRAMKAAGKQKDLEFWTKMTEV

PKYTGPGRLSTFEVKFSCYSRSLFTIDPDNIKAVLTGQFADYGKGRRFHEEWRDFLGDSIFATDGELWSKSRQLIRPMFS

RDRIVDTEIFEKHIHKLIPLLGGGANQDGSKVVDVGPLFFRFTLDAATDYLLGQSVDSLDNPKTTFAESFQYVLHRQSVI

FRSGPLSPLLSRKLFHENLKKMDDFMQPFIEQVRALTPEELDHKLSKKDTFLHALARFTRDPTVLRDQLVAILLAGRDTT

AATLSFCIFELSRHPAVVQRLRNEIETVCGNRKPTYSELKEMKYLNAVLNETMRLYPVVPFNVRHALVDTTLPRGGGSDG

QSTIGVPADTRIVYSTMAMQRRRDLYPPPPVPGEKTLQPYFDPLLFHPERWLSGWQPKPWHFIPFNGGPRICLGQQFATI

EMGYTVTRILQHYPEIIGVGAPKPGTDPKFKFDVTLSPGQRLDCVFVKKEE

 

>CYP539D6P Histoplasma capsulatum G217B

pseudogene

68% to CYP539D4 Uncinocarpus reesii

HCB02885.1

Second half of this model is like annexin (deleted)

ABBT01000091.1 Ajellomyces capsulatus G217B 86855-88176

GQRFHEEWREFLGDSIFATDGELWSRSRRLIRP

MFARDRIVDKENFEKHIQTLIPHLKGNNENPGKGTIVDVATLFFCFTLVAATDYLLGQSVDGLDDPKTIFAESFQYVLHRQSNS

CLPLCRPIRPLLSGKGFRLNLKKMDDFMQPLIDEVLPLSSEEFDKKLSK

KHTFLHALAHFTKDHKVIHDQLVAILPAGRDTAATLCFCLFELSHNPTVVAPEVLDRLGTSRKPSY

SDLKEMKFLTAVLNETLRLCPVVPLNV &

LPRGGGPDGHSSIGIPNNTRIFYSTTNMQRRRDLYDTPPLAPGNAGSDEKWPIPYFEPLL WIPERWTDGWQPRPWHFIPFNGGPRICLGQPFAMLDMGYTVSRILQNFSEIRAGGAGPAGQDQPFK &

FDVTLSPGEELNMVFVEAGKETG*

 

>CYP539D6P H. capsulatum G186AR

Supercontig 8: 874979-876322 +

88% to CYP539D6P Histoplasma capsulatum G217B

GQRFHEEWKEFLGDSIFATNGELWSRSRHLIRPMFARDRIVDTENFEKHIQKLIPHLEKN

NEKPGEETIVDVAALFFCFTLSAATDYLLGQIVDSLDDPKTIVAESFQYVLHHQSNF

CFPLCRPISPLLSSKGFRLNLKKMDDFMQPL

IDEVLPLSSEELDKKLSK

KHTFLHALVHFTKDRKVIHDQLVAVLPAGRDTAATLCFCLFELSHNPTVVAPLHTEVLD

RLGTSRKTSCSDPKEMKYLTAVLNEALRLCPVVPLNVCHALTDTTLPRGGGPDGHSSIGI

PNNTRIFYSTTNMQRRRDLYDSPPPAPGNAGSDEKRPIPYFDPFPWIPERWTAGWQPRP*

HFIPFNGGPRICLGQPFAMLEMSRILQNFNEIIADG

AGPAGQDPPFKFDVTLSPGEELNMVFVEAGQETG

 

>CYP539D7 Aspergillus clavatus

68% to CYP539D3, one frameshift = &

ACLA_082210 revised

MIEDLIAQMPLGMATLYLLGAFFLICLLRKAQVSTQISMLGYRAPRVGFRLPYAIDFIYKALKAHNIHEDLEFWHQAIRT

AKGASKIDHVKTAEVDGGVSNRVILTKDPENIKALLTGQFADYGKGEPFHREWKEFLGDSIFATDGELWSRSRHLIRPMF

ARERIVDTEIFEKHVQHLIPLLAGSDAPGGSKKVDVGSLFFRFTLDAATDYLLGQGTDSLQNPETRFAEAFRYVQSRQSE

LFRLG (2)

VFSFILSKRKFRQELKVMDSFLQPYIDRVLSLTPSELDQKLTKQESFLDALARFTRD

PRVLRDQLVAVLLAGRDTTAATLSFCVFELARNPHVVAKLRQEIDARLGLGPKGRKPTYNDLKEMKYLSAVLNETMRLYP

VVPFNVRYSLRDTTLPRGGGPDGLSPIGVRANTRIIYSTMIMQRSAENYDPPGSPTYFDPEKW (&)

PERWLSAWQPRPWHFIPFNGGPRICIGQQFATIEMGYTIVRILQAF

ESVCALPANGKNRVEDPVLRFEVTLSPGSELNCVFVREGEGQRTGH*

 

>CYP539D8 Aspergillus fumigatus

supercontig 6 1986238-1987987 (+) strand

MIEELLAQIHSGWAALYVLGALFFACLIRKAQVSMEISKLGYRAPRIRFYLPW ()

AIDFLYEASKANEDGTDHVFLHQAILNAKGATQLDCVKTAELNGG

ITRRVILTKDPENIKAILTGQFADYGKGEEFHEQWKDFLGDSIFATDGELWSRSRHLIRP

MFVRDRIVDTEIFEKHVQNLIPLLEGSNSPSGSKAVDVGSLFFRFTLDAATDYLLGQGTN

SLHNPETRFAEAFGYVKHRQSEIFRLG (2)

MFSFIVAKRKFRRELKVMDDFFQPYIKRALSLTPSELDQKISKRETFLDALARFTRDPRV

LRDQIVAVLLAGRDTTAAVLTFCIFELARNPDVVAKLREEISARLGLGPSAQKSSYNDLK

EMKYLNAVLNETMRLYPPVPFNVRYSLRDTTLPRGGGPDGLSPVGVRANTRVIYSTMIMQ

RSAENYDPPGSPNYFDPEKWLPDRWLSGWQPKPWQYIPFNGGPRICLGQQFATIEMGYTV

VRILQAFERIHAMPANGKERVEDPVLKFEVTLSPGSELNCVFVREGEDTLPLKDG*

 

>CYP539D8 Neosartorya fischeri

95% to CYP539D8 Aspergillus fumigatus = ortholog, 79% to CYP539D7

NFIA_054110

MIEELLARIHPGRAALYVLGALFFACLIRTAQVSMEISKLGYRAPRIRFCLPWAIDFLYGASKANDDGTDHVFLHQAILN

AKGATQLDCVKTAELNGGIARRVILTKDPENIKAILTGQFADYGKGEEFHEQWKDFLGDSIFATDGELWSRSRHLIRPMF

VRDRIVDTEIFEKHVQNLIPLLEGSNLPSGSKVVDVGSLFFRFTLDAATDYLLGQGTNSLHNPETRFAEAFGYVKHRQSEIFRLG (2)

MFSFIVTKRKFRRELKVMDDFIQPYIKRALSLTPSELDQKISKRETFLDALARFTRDPRVLRDQIVAVLLA

GRDTTAAVLSFCIFELARNPDVVAKLRKEIGARLGLGPSAQKPSYNDLKEMKYLNAVLNETMRLYPPVPFNVRYSLRDTT

LPRGGGPDGLSPVGVRANTRVIYSTMIMQRSAENYESPGSPNYFDPEKWLPDRWLSGWQPKPWQYIPFNGGPRICLGQQF

AAIEMGYTVVRILQAFERIQAMPANGKERVDDPVLKFEVTLSPGSELNCVFVRKGEGTLPLRED*

 

>CYP539D9 Aspergillus flavus

75% to CYP539D7 Aspergillus clavatus

AFL2G_09153

MIEELLHQMTPGKASVLFLGVFIVLCLVRKVQVSMHIKRLGGRAPQIQFRFPY AMDFIYNSLNANRAHRDLEFFGNAIKT

AKGASQIDSPKTAEVSAGISSRFIFTRDPENIKAILAGQFADYGKGEPFHNDWKEFLGDSIFVTDGELWSRSRHLIRPMF

VRERIVDTEVFEKHVQHLIPFLSGGNSPAGSKIVDVGPLFFRYTLDAATDYLLGQGTDSLQNPTTSFSEAFGYVQHRQAD

LTRLG IFKFLLSLREFRRNLKIMDNFIQPYINRVLSLSTAELDQKLSKQDTFLDSLARFTRDPRVLRDQLVAILLAGRDT

TAGTLSFCLFELARNPQVVAKLRQEIESRLGVGAKGRKPSYIDLKEMKYLNAVLNETMRLYPVVPFNVRLALRDTTLPRG

GGPDGLSPIGVRADTRVLYSTMLMQRNPENYDAPGSPGYVDPEKFHPERWLSGWQPKPWRFIPFNGGPRICLGQQFATIE

MGYTVVRILQAFREIKAFPVSGKSVVEDPVLRFEVTLSPGSELNCLFVPEEASPTK

 

>CYP539D9 Aspergillus oryzae

Supercontig 5: 1308953-1309825 (+) strand

100% to CYP539D9 Aspergillus flavus

MIEELLHQMTPGKASVLFLGVFIVLCLVRKVQVSMHIKRLGGRAPQIQFRFPY ()

AMDFIYNSLNANRAHRDLEFFGNAIKT

AKGASQIDSPKTAEVSAGISSRFIFTRDPENIKAILAGQFADYGKGEPFHNDWKEFLGDSIFVTDGELWSRSRHLIRPMF

VRERIVDTEVFEKHVQHLIPFLSGGNSPAGSKIVDVGPLFFRYTLDAATDYLLGQGTDSLQNPTTSFSEAFGYVQHRQAD

LTRLG ()

IFKFLLSLREFRRNLKIMDNFIQPYINRVLSLSTAELDQKLSKQDTFLDSLARFTRDPRVLRDQLVAILLAGRDT

TAGTLSFCLFELARNPQVVAKLRQEIESRLGVGAKGRKPSYIDLKEMKYLNAVLNETMRLYPVVPFNVRLALRDTTLPRG

GGPDGLSPIGVRADTRVLYSTMLMQRNPENYDAPGSPGYVDPEKFHPERWLSGWQPKPWRFIPFNGGPRICLGQQFATIE

MGYTVVRILQAFREIKAFPVSGKSVVEDPVLRFEVTLSPGSELNCLFVPEEASPTK

 

>CYP539D10 Aspergillus terreus

76% to CYP539D9

ATEG_05807.1

MIEEFITQMSPGRAALCFLGALVVLFTLRKMQVSMKIARLGGRAPQVRFYLPYAMDFIYRSSKANREHRDLEFFTEAMRT

AKGAAALDNPKTAEVNAGMAARVIFTKDPENIKALLTGQFADYGKGESFHQDWREFLGDSIFATDGELWSRSRHLIRPMF

VRDRIVDTEIFEKHVQHLIPLLEGSSSPTGSKIVDVGELFFRFTLDASTDYLLGQGTDSLQNPETEFAEAFRYVQHRQAL

FFRMGVFGPLLSRKKFREQLKVMDDFIQPYIDRVLSLSTAELDQKLSKKETFLDALARFTRDPRVLRDQLVAVLLAGRDT

TAGTLSFCLFELSRNPEIVAKLREEIRSRVGLGANGQKPTYNDLKEMKYLNAVLNETMRLYPIVPFNVRFSLRDTTLPRG

GGPDGRAPIGVLEGTRVIYSTMIMQRSPEFYPPPGSPNYFDPQKWHPERWISGWQPKPWQFIPFNGGPRICIGQQFATIE

MGYTVVRILQAFEQVQGIPAAGKNRIEDPVLQFEVTLSPGSELNCIFVRDGEGIKESETG*

 

>CYP539E1 Fusarium graminearum FG08005.1 AACM01000323 FGcontig1.323_scaffold5

MDEIGPDFPFKATPKAVPATLKIDHDCGVKITTSPAINNPPLPADGPGNE

TFSNGLLISLLILVPTCTVWKLGGRFKTTVFFAVITTLPILITFCAITSA

TAPCTNERVKCPGLPVEHYLTFHKEEDQVKYRGRNKVPMETFMRKYFDGD

ADFNGDVLEVLEYRHDWASFRFTWELFRYIIVNFVTDVTAKYGLRWFKIW

KYFLASATTSSRQGSATCYQITLVKNINSVRRIDGVSTQFALSTALEASK

KAGRAVFPANNPSR

P450 STARTS HERE

LQPVFNRERISDLECVERHVQNLLPLLDTEGQTAEI

KDLFLRFTLDVSADFTLGVDLDTLKRPL

NRFAESFERLRHWKIQKERSNP

FKFLVLRSRYQADLDYIESFMDPIIMETVNEIKAEEAGETPHKKDSGFNL

LGASAEVSTNRRFLRDELLTALFAGRDNTAMAFTWILYELAQHPDVVRDM

RREIDAQIGLNSEPGYKTLKDMRILSNIINETLRLYPPVPLNTRAC

110308 LKDTSLPRGGGPLGNDPIGVLKGTGIINSNCL 110213

110192 EAYSTVPEGFPPADEWCPKRWNNWFPKPWAFVPFHGGPRFCLGQQLVLVEMSYTLVQLFQ 110013

110012 RFSRFELRIDE (0)

PRMVTEIALFPRREVRVAFVR*

 

>CYP539F1  Aspergillus fumigatus Af293

          GenEMBL XP_747469.1

          51% to 539B2, 50% to 539A3, 39% to 539C1,

          43% to 539D1, 39% to 539E1

          probably needs some revision

MQGNQMTTTTTTILDYLLLLSLSSYLLKTILGNLSSLKEGFTSLWALKIIGCLFLTIVARPIWNILFSSH

TPRDSKLPQIRTFLPLGIDYITRGIIYSAQNKSLVFWEELFRKYHPFQTLEVRLGPQTVIVSRDPQVVKA

LLTTQFGDFGKGERFHTEWREFLGDAIFTTDGDKWHASRALIRPMFTPDRVSNLSTFERHVQKLLRILES

PRHERQPVNVLDLCLRLTMDIATDFLLGQSVDSLSNPTHRFSAAFADVQRIQSWITMAGPLQVFLPKAKY

YEGLKTINSFVDPFITRTLALGRRSFEEMEGSEEEYNFLEGLATFTQDPKVIRDQLISVLLAARDTTAAT

LARTLYELSGHPDVVQRLREEILAQVGTASAPTYADLKNMRYLQAIHKETLRLYPAIPFNMRVALSDTTL

PSGGGPSGTQPVHVKKDTIVAYSPLYMHRSDLYPDAYADGTPFSDPREYHPHRWMYPGSKTADSEKINEG

PWIPKPWTYIPFNGGPRICLGQQFALAEMGYTLVRIFQKYKCLERRMRAEDGGLMRANIVLTPAQEVNVV

FIQSLSTSGAVMLILYRLRS

 

>CYP539G1 Chaetomium globosum CBS 148.51

NZ_AAFU01000716 partial seq 50% to 539B1 

MLQFLINLGLLHTSALVLG

CLSIFFLSRKLLVDYKIRKLGGVRAPVLATNPVT (1)

GLRFFLWAGYAQTKNRLDHFYRDIFNWATPECPNCVEISFL

GRTRFLMTREPEHIKTILTTKFREYGKGEQFHEAWSPFLGDSIFTT

DGQKWSESRALIRPMFIKDRVRDLEIFDKWA

TTLISKLPASGQTVDVMDLFYRMTLDVTTDFLLGGSVDSLNK (2)

PKHEFVSAFQDVQRIQMLLTIMAYATLIL

PTSPFRALIPTHRYTRGIRTLERFISPFIERTLSLPPSELDPTTNTTTTTTPNKTTTFLH

HLALRARSPQAIP*KIMGGLIAGQPTTAATLTW

 

>CYP539H1 Fusarium verticillioides

50% to CYP539F1 Aspergillus fumigatus, not found in the F. oxysporum genome

FVEG_05544

MSDTVISLVDSSIYMLHAAIHHPYRLALYIIVIYFTRRILNRLLVPPP

ISVAPRIPTYLPFGIDFIVWSSYYNSTNQDLKLWSMIFRRYGRGTAPYTAEV

VLGGDRVIFTADHENIKAVLATKFSDYGKGPKFYHDWKEFLGDSIFATDGR

LWQDARGLIKPLFLRQRIEDLAVFEKHTVKLTRMLQGPEGQPIGVDVKD

LFFRFTLDVATDFLFGQAINSLDNPREQFAVAFAEIQRVQRLITVSGPL

QSFVSLKSYHSSLNTINKFMEVFISETLSLERDELEKLTKSDRGYTFLSAL

ALYTRDAKQIRDQLVAVLLAGRDTTAATLSWVLHELSGHPDMVRRLRQEIA

QHVKPGQNPSFAELKAMKYLQAILNETLRMYPAIPFNMRIALDDTSLPR

GGGSDGNQFISVKKDTIIAYTPLVMMRRNDLYPATYNNGDKFPDPNTFD

PERWLHPSVLHTAEKGADASETKASGVNNKEWSPRPWTYIPFNGGPRICLG

QQFALTEMGYTLVRIFQQFARVERRMEPDEMGILSANIVLTPKNGVRVAFFKEEED*

 

>CYP539J1 Aspergillus terreus

48% to CYP539A6 Mycosphaerella graminicola

ATEG_08167.1

MMLGEAGQFPHMG

ALLLTALFIVYLCRQWTTRQKILRLGGYAPRIPSYVPL (1)

GLDILFTAIRNGRRNKSLETWRGWHSCAPNNGTVEAKVLFERYLLTGDPENIKAILATQF

DDFAKGQLFHDTWSQLLGDGIFSTDGAPWRGSRQLMRPLFAKDRVSDLDVLERHVQKLLR

NVAAKSGQRLDLCDLLFRYTLDAATDFLLGEPVGSLDNDTQEFATAFDHAQYIQMMIARSG (2)

RAHVLIPRASLNKSISTINAFIRP

YIDSALRLQDSTKLVERSEPDYTFLHALAHYTRDPKLIRDQLVNILIAGRDTAASSLS

WVFFELAKQPRVTTKLREEILAQVGFERPTYSQLKDMKYLQ (0)

NVIRETLRLYPIIAF

NVRVAQRDTTLPRGGGPDGLSPVGVLKGTRVFFSSLYMQRRASLYPGDPNPDEFHPERWE

KWQPASWQYIPFSGGPRICIGQQFALTEIAYTIVRLLQSFDRIEPCIEHMEPVLETNISM

KPGTGVPVKLWSAAPVSNEQRVEGSAC*

 

>CYP540A1 N. crassa AABX01000009.1 cont3.484 NCU08062.1 (version3)

46350 MSSQTFFLVGDGPSTARGIVVDPKWKLEELKRAVGLAHHIAVPTG (1) 46481

46537 GITFHFDADDEQELKTVEDVLRASGPVACRIDGNPVRDPQGPEGLPIVGS

YYEIFPDHLGNHYRLFRKYGHVIKTTNMGRTNYLTDDPAVALVALAESAYFTKKIT

EEHPLFGIKDNSAIFIGDTETENWRLAHKFLPPAMGPKAVRHYTPLMQEC

VRGSFKIFDELDSRGESWNVYQYMVKLASQTVGKFAMGIDYHHFDAVDTP

VHSLVTNIVKLLALNKKVTARGSWYQQLPFGDPARLREIRQITYGQLAET

IEAAPKSGIENLPLNEAATKASCVADYLLNAVDEKGEHFPKGLILSNMLV

VTGAGYTTTSALLSWCIYCIVTYPKMQDRLLQELIDHGINSETDWDPDLA

HSLPFLDAFLKETQRLHNASFQPGRTTKTDVVLPGGFRLPENVIMIPALY

AIHTNPEHWHDPFRFDPDRWDTEEVKSRHRGAYMPFATGPRSCIGFNFAL

LEIKVLLSELVYRYKFAREGFEAIEYDPEFQLIRPLNLYVTAKRRTEWPAKSSA* 48069

 

>CYP540A2 Aspergillus nidulans AN8919.1 68% to 540A1 check introns 505 clan

MRPPTVQCQFYLIGDEPSSAQSVTVDTRWKFEDLQRAVGLVFHVAQPLGT

LSTVDEVLTLANSSSAIGILVDGKPVQSPQGPPGLPLVGSFYEIFPDHLG

NHYRLFRKYGPVIKTTNMGKTIYLTDSPQVAASCFAESIYMTKKITEDHP

LWGVKDNTAIFIGDTETENWRLAHKYLPPAMGPKAVRHYTGLMQNCARKA

LPVFDELDARDESWNVYQYMVKLASQTIGSFSLGKDFGHFASIDSPLHPI

VTNIASLLSLNKKITARGEWYRLLPFGDPARLRSVQHTIYTLLQEAIDEV

AARSASKDAPMSDAALSASCVVDYLLHAVDEKGEHFPTGLILANMLIVTG

AGFTTTSALLSWLIYCLVTYEGTQDRLYSELVEYGIVGPSGEPNKTTWTP

DLAHSLPYLNNFVKETQRLHNASFQPGRTTKTDVVLPGGYRIPENSVIVP

ALYAIHTNPSIWHDPFRFNPDRWDSDEVKNLHRCAYVPFATGPRGCIGFN

FALLEVKIILAELVSRYEFVRDGVESIEYDPEFQLIRPLNFYVRAKRRS*

 

>CYP540A3  Aspergillus oryzae

          GenEMBL BAE57130.1

          84% to 540A2, 56% TO 540B9

MKCQFYLVGDDIATAQSILVDSRWKFEDLQRAVGGIFHVALPTGISFHTSENETLSSVADIISASSSPIG

LRIDGNAVQTPQGPKGLPLVGSFYEIFPDHLGNHYRLFRKYGPVIKTTNMGKTTYLTDDPQVASVCLAES

AYMTKKINENHPLWGVKDNTAIFIGDTETENWRLAHKYLPPAMGPKAVRHYTGLMQNCARKSLPVFDELD

GRDESWNVYQYMVKLASQTIGSFSLGKDFGHFDSVDSPLHPIVTNIANLLSLNKKITARGEWYRYLPFGD

PARLRHVQHTIYTLLQEAIDEVAGSGIADAPMNEAALSASCVVDYLLHAVDDKGEHFPQGLILANMLIVT

GAGFTTTSALLSWLLYCLVTYVGTQDRLYAELVEHGIVGPSGERNQTTWTPDLAHSLPYLDKFVKETQRL

HNASFQPGRTTKTDVVLPGGYRLPPDSVIVPALYAIHTNPKTWRDPFRFDPDRWDTEEVKGRHRCAYIPF

ATGPRGCIGFNFALLEVKILLAELVSRYEFVRDGLEAIDYDPEFQLIRPLNFYVRAKRRV

 

>CYP540A3  Aspergillus flavus

99% to CYP540A3  Aspergillus oryzae

AFL2G_09274

MKCQFYLVGDDIATAQSILVDSRWKFEDLQRAVGGIFHVALPTGISFHTSENETLSSVADIISASSSPIGLRIDGNAVQT

PQGPQGLPLVGSFYEIFPDHLGNHYRLFRKYGPVIKTTNMGKTTYLTDDPQVASVCLAESAYMTKKINENHPLWGVKDNT

AIFIGDTETENWRLAHKYLPPAMGPKAVRHYTGLMQNCARKSLPVFDELDGRDESWNVYQYMVKLASQTIGSFSLGKDFG

HFDSVDSPLHPIVTNIANLLSLNKKITARGEWYRYLPFGDPARLRHVQHTIYTLLQEAIDEVAGSGIADAPMNEAALSAS

CVVDYLLHAVDDKGEHFPQGLILANMLIVTGAGFTTTSALLSWLLYCMVTYVGTQDRLYAELVEHGIVGPSGERNQTTWT

PDLAHSLPYLDKFVKETQRLHNASFQPGRTTKTDVVLPGGYRLPPDSVIVPALYAIHTNPKTWRDPFRFDPDRWDTEEVK

GRHRCAYIPFATGPRGCIGFNFALLEVKILLAELVSRYEFVRDGLEAIDYDPEFQLIRPLNFYVRAKRRV

 

>CYP540A4 Aspergillus niger

fgenesh1_pg.C_scaffold_1000410|Aspni1

85% to CYP540A3

MQCHFYLIGEAVATAQCLEVDTLWKFEDLQRAVGGVFHVVQPTGISFHASSNDTLKTVSEVLAAATSTSPVGLRIDGNAV

QAPQGPPGLPLVGSFYEIYPDHLGNHYRLFRKYGPVIKTTN MGKTIYLTDDPQVAAACFAESAYMTKKINSDHPLWGVKD

NTAIFIGDTETENWRLAHKFLPPAMGPKAVRHYTGLMQECARKSLPVFDELDVRDESWNVYQYMVKLASQTIGRFSLGKD

FGHFDAVDSPLHPIVTNIASLLSLNKKITARGEWYRHMPFGDPARLRHVQHTIYSLLQEQVDTVTAESCSTDAPMADAAL

SATCVVDYLLHAVDETGAHFPPSLILANMLIVTGAGFTTTSALMSWLIYCLVTYEGTQDRLYAELVEYGIVGPTGERNET

AWTPDLAHSLPYMDRFVKETQRLHNASFQPGRTTKTDVILPGGYLLPPNSVIVPALYAIHTNPKVWRDPFRFDPDRWDTD

EVKSRHRCAYVPFATGPRGCIGFNFALLEVKVLLGELVSRYEFVRDGQEAIDYDPEFQLIRPLNFYVRAKRRA*

 

>CYP540A5 Aspergillus terreus

87% to CYP540A4 A. niger

ATEG_06722.1

MQCHFYLVGDDVSTAQSLDVDARWKFEDLQRAVGGVFHVAQPTGISFHSDSNEPLTTVAD

VLTAASAPTAPVGLRIDGNAVQSPQGPPGLPLVGSFYEIFPDHLGNHYRLFRKYGPVIKTTN

MGKTIYLTDDPAVAAVCLAESAYMTKKINSDHPLWGVKDNTAIFLGDTETENWRLAHKYLPPSMGPKAVRHYTGLMQACA

RKSLPVFDELDARDEAWNVYQYMLKLASQTVGSFSLSKDFGHFDSIDSPLHPIVTNIASLLSLNKKITARGEWYRHLPFG

DPARLRHVQHTIYSLLQEQIDAVAAESRSADAPMADAALSATCAVDYLLHAVDDKGERFPPGLILANVLVVTGAGFTTTS

ALLSWLIYCLVTYEGTQDRLYQELVEYGIVGPNGERNQTTWTPDLAHSLPYMDKFVKETQRLHNASFQPGRTSKTDVVLP

GGYRLPPDSVIVPALYAIHTNPKIWRDPFRFDPDRWDTDEVKNRHRCAYVPFATGPRGCIGFNFALLEVKILLGELLSRY

EFVREGQEAIDYDPEFQLIRPLNFYVRAKRRV*

 

>CYP540B1 Magnaporthe grisea MG10724.4  52% to CYP540A1 AACU01001777 cont2.2064

MSSKLFYLLGNEPLTAIEIEIPSDTNDEGLQHLLASHFAIVEPKGVGYVS

GDVVLTSVPEVLAAQGPISVTIDGKPIRDVPGPTGLPYIGNYFEVYPDHL

GNNQRLFDRYGPLFKTTNMGSVVYHTNDPQLAGIFFSESDFFTKKMIPGH

PLHPIKNKEAGVFLGDTDTEEWRVAHKFLPPALGPAAVRHYAPTMQSTVE

DAFKVFDQLDERGEAWNAYWAMLKLGSQAVGKLVLGMDFKHFEKVDSPPH

EMVIRIAQSLELNKKVTSMGSWYAKMPFGAPQQLRNARERIEQMMIESVE

NASKGDEDLELQQAALTANNMVDYVLRAKDSKGNKLPRDRILEPLVVATG

AGFTTTSSLLSWLLYGLVTYPGVQDRLLQELVDNGFDHETKMTADFTQKL

TFLDYYIKETQRMHNPSYQPARTAKADMILPGGYRLPKDAIVIPALHSLH

KNPLYWDNPQRFDPDRWGTEAVKNRPAGSYAPFGMGQRMCIGFNFALQEV

KVFLPKLLYRYEFESATEGNVEYDPMFQLIRPTNLYLRARRRVKWPPRSE

EATAAAC*

 

>CYP540B2 Fusarium graminearum FG02138.1 AACM01000111 FGcontig1.111_scaffold1

MSTKRFHLLGEDPSTAQQIELPPSLDEQGLQHLVASHFAIVDPSVTNFSG

IGFVTDSEALPTVADVLAADDISITIDGKAVREVPGPSGLPFVGNYFEVY

PDHLGNHQRLFEKYGPLFKTTNMGNTIYQTNDPKLATIVFGESDFFSKRI

IEGHPLFPIKNKEAGVFVGDTDTEEWKEVHKFLPPALGPKAVRHYAPTMQ

RTVEDAFKVFDELDERDEAWNVYPYMLKLGAQAVGKLVLGMDFQHFSSPD

ARPHAMVLRIAQSLELNKKLTSMPSWYKSLPFGDPQRMREARAHMEAMMT

ESVANAAKGEGDLELQDAAVEAENMVDYVLRASDSKGNKLPRERIMEPLV

VATGAGFTTTSSLLSWLLYGLVVYPGMQERLLQELVDNGFDENTQITADL

TNKLTFLDKYIKETQRRHNPSYQPARTSAVDMILPGGYKLPKDSIVIPAL

HHIHNNTKIWDNPALFDPDRWDTDRVKNRPKDSYIPFATGPRMCVGFNFA

LQEVKVFLPKLVYRYKFNLAQDGPVEYDPMFQLIRPNNL*

 

>CYP540B3 rev Fusarium graminearum FG03548.1 AACM01000159 FGcontig1.159_scaffold2

MSTKQFYLLGEVVSTARDIELPPAVDFEELQSIVASHFAIVKPNSK (2)

VGFIHDGRKLNAVSEILE

VNESIAISVDGNAVRDVPGPAGIPYFGNYLEIYPDHLGNHQRLFEKYGPL

FTTTSMGNRLYQTNSAELSNIFLSEDHYFTKDIVPGHPLHPIKNQEAGVF

LADTNTEQWRLAHKFLPPALGPKAVRHYAPTMQRTVESSFKVFDELDQKG

EAWNVYQYMLKLGSQAVGKLVLGMDFAHFEQVDSPLHEMVLKIAENLELN

KRVSSMGAWYAQMPFGDPKKVRQTMARIMEMMDESIARASKGQEDLELQD

AALKADNVVDYFLRATDNKGNKLPPSQFAPALLVATAAGFTTTSSLLSWL

IYSLVKYHGNQERLLQELIDNDWDENTQVTADTTSKLTFLDKFIKETQRL

HNPSFQPGRTAKVDMILPGGYRLPKGAVVISALHHMHNNKDVWENPGRFD

PDRWDTEQVKNRPPGSYIPFATGPRMCVGFNFALQEIKVFLPKLVYRYKF

SLAQDGPIEYDPYFQLIRPNNLYVQAEKRVKWPPKSE*

 

>CYP540B4 Aspergillus nidulans AN3861.1 67% to 540B2 505 clan

revised 7/18/07

MLNQQFYLHGETASSAKSITLDETANLDQVKHIVAAHFAIVEPNGIGFQT

ENDCLVDVSSILTAPGPIAITIDGRAVREPEGPKGLPFVGNYFEVFPDHL

GNHQRLFDTYGPIIKTNNLGRTTYQTNDPQLSAIVLAESDFFTKKINEAH

PLYPLKTPEAGVFLGDTDTKEWRDAHKFLPPALGPKAVRHYAPTMDSCVK

DAFKVFDALDETGEAWNVYQYMLKLGSQAVGKLTLGLDFEHFTSPDAPTH

EMVHAIAELLSLNKEVTSRGDWYAKLPFGAPKRLRNLKARIEEMVDESVQ

RAARGGVSDLPLQEAALQASNMVDYAIRATDNKGEKLPKSSLIWALVVAT

GAGFTTTSSLLSWLIYGLVTYPGMQDRLLQELVDHGITEDTELTADLTEN

LPFLDKYIKEMQRRHNPSYQPGRTAKVDLVLPGGYKIPKDSVVIAALHHI

HNNPSIWDNPSRFDPDRWDTDEVKQRHKASYIPFAIGPRMCIGFNFALSE

IKVFLPRLVYRYNFIREGDGPIEYDPMFQLIRPNNL

YVRAERRVKWPAKTKSSE

 

>CYP540B5 Nectria haematococca

fgenesh1_pg.scaffold_13000341

Necha1/scaffold_13:983726-985420

missing 5 aa between DPN and GIG compared to 540B2

540B2 may be too long here

87% to 540B2

This gene model seems correct

MATKRFYLCGDSSTAQDIELPSSLDEQSLQHLVASHFAIVDPN (1)

GIGFINQSEALTTVADVLAAEDVAITI

DGKAVREVPGPKGLPFVGNYFEVYPDHLGNHQRLFEKYGPLFKTTNMGSTIYHTNDPALANIVFGETDFF

SKRIIEGHPLFPIKNTEAGVFLGDTDTPEWKEVHKFLPPALGPKAVRHYAPTMQKTVEDAFKVFDELDER

DEAWNVYPYMLKLGSQAVGKLVLGMDFKHFTAVDARPHEMVLRIAQSLELNKKITSMGSWYRNMPFGDPQ

RLREARARMETMMEESVANASKGVGDLDLQDAALQAENMVDYVLRASDSKGNKLPRNRIMEPLVVATGAG

FTTTSSLLSWLLYGLVVYPGMQERLLQELVDNGFDDDTQITADVTNKLSFLDKYIKETQRRHNPSYQPAR

TAAVDLILPGGYRIPKDAIVIPALHHIHNNTKIWDNPALFDPDRWDTERVKNRPNASYIPFATGPRMCVG

FNFALQEVKVFLPKLVYRYKFSLAQDGPVEYDPMFQLIRPNNLYVRAERRVKWPAKSESL*

 

>CYP540B6 Nectria haematococca

fgenesh1_pg.scaffold_23000209

Necha1/scaffold_23:527402-529200

60% to 540B2 some revision at N-term, add cyan and delete some

MPLQSFYLLGEDPETAREIDVDQNASFNHLQQLIASHYAIVVPT (1)

GIGFQGTDNALSTASQVMSTKDKVAITIDGRAVRDIPGPPGLPYVGNF

FEVFPDHLGNHQRLFERYGPVIKTTNLGRTVYHSNDPEVAAVAFAESEFFTKRINDAHPLNGIKTPEAGI

FLGDTDTPEWRVTHKFLPPALGPKAVRHYAPTMQKTVEKAYEVFDLLDEREEAWNVYQYMLKLGAQAVAK

LTLGLDLEHFSSVDAPVHEIVHLIADMLSLNKKVTSRGNWYASLPFGDPKALRDKKATLERMVEERIQQA

KAGGIEDLPLQDAALRATDMI (1)

DYALRATDNKGERLPKSSLTWALVVATGAGFTTTSSLLSWLIYGLVTYP

DVQDKLLQELIDHGFTDDMEVTSDFTDRLVYLDYYIKETQRRHNPSFQPGRTSKVDLILPGGYKLPKDSV

MIPALHHIHNNPEFWDNPAKFNPDRWGTDEVKNRHKAAYLPFGTGSRMCIGFNFALQEIKIFLPKLIYRY

RFRKDGSDVTEYDPMFQLIRPNNLYVRAEKRTKLPPKSDTAVSST*

 

>CYP540B7  Aspergillus fumigatus Af293

          GenEMBL XP_753637.1 also EAL91599.1

          76% to 540B4

MASQQFYLLGESISSARTISLDGINDLEGLRNLIASHFAIVEPSGIEFSRQDVSLTDLHEITSATTPVAI

TIDGHAVRNAPSPKGLPFFGNFFQIFPDHLGNHQRLFEQYGPLIKTNTLGRTVYQTNDPILSAIVFNESD

FFTKKINEAHPLYSLKTPAAGVFLGDTDTPEWRVAHKFLPPALGPKAVRHYAPTMQKTVEDACTIFDQLD

AQGEAWNVYQYMLKLGSQAVGKLTLGLDFEHFSSADAPIHEMVHLIAEVLSLNKKVTSKGDWYASLPFGD

PKRLRDTKARIEQLVEESVQKAQRGGVEDLPLQDAALRAANMVDYALRATDNKGEKLPKSSLVWALVVAT

GAGFTTTSSLLSWLIYGLVTYPGMQDRLLQELVDHGFDDTTEITADFTDQLTFLDKYIKETQRRHNPSFQ

PGRTAKLDLILPGGYKIPQDAVVIPALHHIHNNPAIWDNPARFNPDRWDTDEVKQRHKAAYIPFGTGPRM

CIGFNFALQEIKVFLPKLIYRYKFTREGDGPIEYDPMFQLIRPNNLYVRAERRVKWPYKSDA

 

>CYP540B7 Neosartorya fischeri

96% to CYP540B7  Aspergillus fumigatus = ortholog

NFIA_077230

MASQQFYLLGESISSARTISLDGMNDLEGLRNLIASHFAIVEPSGIEFSGQDISLTDLNEITSSTTPVAITIDGHAIRDA

PSPNGLPFFGNFFQIFPDHLGNHQRLFEQYGPLIKTNTLGRTVYQTNDPVLSAIVLNESDFFTKKINEAHPLYSLKTPSA

GVFLGDTDTPEWRVAHKFLPPALGPKAVRHYAPTMQKTVEDACTIFDQLDAQGEAWNVYQYMLKLGSQAVGKLTLGLDFE

HFSSADAPIHEMVHLIAEVLSLNKKVTSKGDWYASLPFGDPKRLKDTKARIEQLVEESVQKAQRGGVEDLPLQDAALQAA

NMVDYAIRATDNKGEKLPKSSLVWALVVATGAGFTTTSSLLSWLIYGLVTYPGMQDRLLQELVDHGFDDNTEITADFTDK

LTFLDKYIKETQRRHNPSFQPGRTAKLDLILPGGYKIPQDAVVIPALHHIHNNPAIWDNPARFNPDRWDTDEVKQRHKAA

YIPFAMGPRMCIGFNFALQEIKVFLPKLIYRYKFTREGDGPIEYDPMFQLIRPNNLYVRAERRVKWPYKSEA*

 

>CYP540B8  Aspergillus fumigatus Af293

          GenEMBL XP_748718.1 also EAL86680.1

          75% to 540B7, 73% to 540B4

MSTTTTKLFHLLGEPLSTAKEIQFESTTTYGDLRHLVAAHFAIVEPSGIGFISQDSILHDVPEIAASDEI

ISITIDGKAVREIPGPKGLPVIGNFFEVYPDHLGNHQRLFEQYGPIFKTTNMGRTVYHTNDPQLSSIIFA

ETDFFTKKINAAHPLHPIKNQEAGVFLGDTDTPEWRTAHKFLPPALGPKAVRHYAPMMQETVEDAFTVFD

ALDERGEAWNVYQYMLKLGSQAVGKLVLGIDFKHFSSIDEPPHELVYRIAESLELNKKVTAWGDWYAKLP

FGDPQRLRNARGRIIEMVNESIQNAARGGIEDLPLQDAALKASNMVDYALRATDNKGEKLPKTSLMQALV

VATGAGFTTTSSLLSWLIYGLVTYPGVQERLLQELIDNGIDADTQLTADLTDRLTFLDKFIKETQRRHNP

SYQPGRTAKVDMILPGGYKLPQDSVVIGALHHLHNNPDVWSNPARFDPDRWDTEEVKNRHKTAYIPFATG

PRMCIGFNFALQEVKVFLPKLVYRYKFTRENDGHIEYDPMFQLIRPTNLYVRAERRVKWPPRSDNAVPSP

SL

 

>CYP540B8  Neosartorya fischeri NRRL 181

           DS027697 join(1677302..1677440,1677496..1678332,1678385..1679094)

           73% to CYP540B4, 96% to CYP540B8 Aspergillus fumigatus = ortholog

           name revised from CYP540B11

           NFIA_006270

MSTTTKLFHLLGEPISTAKEIQFESMTTYEDLRHLIAAHFAIVE

PNGIGFLSQDSILHDVPEIAASDDIISITIDGKAVREIPGPKGLPFIGNFFEVYPDHL

GNHQRLFEQYGPIFKTTNMGRTVYHTNDPQLSSIIFAETDFFTKKINAAHPLHPIKNQ

EAGVFLGDTDTPEWRTAHKFLPPALGPKAVRHYAPMMQETVEDAFNVFDALDEKEEAW

NVYQYMLKLGSQAVGKLVLGIDFKHFSSIDAPPHELVYRIAESLELNKKVTAWGDWYA

KLPFGDPQRLRNARGRIIDMVNESIQNAARGGIEDLPLQDAALKASNMVDYALRATDN

KGEKLPKTSLMQALVVATGAGFTTTSSLLSWLIYGLVTYPGVQERLLQELIDNGIDAD

TQLTADLTDRLTFMDKFIKETQRRHNPSYQPGRTAKVDMILPGGYKLPQDSVVIGALH

HLHNNPDVWSNPARFDPDRWDTEEVKNRHKTAYIPFATGPRMCIGFNFALQEVKVFLP

KLVYRYKFTRENDGHIEYDPMFQLIRPTNLYVRAERRVKWPPRSDKAAPSPSL

 

>CYP540B9  Aspergillus oryzae

          BAE62604.1

          80% TO 540B10, 69% to 540B1

          revised 3/20/2009

MPIRLFYLSGEPSSTAREIELESTLDYDGLRHLIAA

HFAIVEPN (1)

GIGFQSQDAILSDVAEVLANE

DSISITIDGKSVREISGPKGLPIVGNFFEIYPDHLGNHQRLFEQYGPIIKTTNMGRTVYQTNDPQL

SSIIFSETDFFTKKINEAHPLHAIKNQQAGVFLGDTDTPEWRAAHKFLPPALGPKAVRHYAPTMQQTVED

AFKVFDHFDENGEAWNVYQYMLKLGSQAVGKLVLGIDFNHFSSPDAPPHELIQIIAESLSLNKKVTARGD

WYAKLPFGDPQRLRNMRYRIFDIVDQSIQNASRNGVEDLPLQDAALKASNMIDYAVRATDNKGEKLPKTS

LIQSLVVATGAGFTTTSSLLSWLIYSLVTYPGVQDRLLQELIDNDITEDTQITADLTDRLTFMDKLIKET

QRRHNPSYQPARTAKVDMILPGGYKLPEESVVVGALHHLHNNPEVWSNPTRFDPDRWDTEEVKNRHKAAY

IPFATGPRMCIGFNFALQEVKVFLPKLVYRYKFTKENDGPIEYDPMFQLIRPNNLYVRAERRVKWPPKSE

SATTRASL

 

>CYP540B9  Aspergillus flavus

99% to CYP540B9  Aspergillus oryzae

AFL2G_07990

MPIRLFYLSGEPSSTAREIELESTLDYDGLRHLIAAHFAIVEPNGIGFQSQDAILSDVAEVLANE

DSISITIDGKSVREI

PGPKGLPIVGNFFEIYPDHLGNHQRLFEQYGPIIKTTNMGRTVYQTNDPQLSSIIFSETDFFTKKINEAHPLHAIKNQQA

GVFLGDTDTPEWRAAHKFLPPALGPKAVRHYAPTMQQTVEDAFKVFDHFDENGEAWNVYQYMLKLGSQAVGKLVLGIDFN

HFSSPDAPPHELIQIIAESLSLNKKVTARGDWYAKLPFGDPQRLRNMRYRIFDIVDQSIQNASRNGVEDLPLQDAALKAS

NMIDYAVRATDNKGEKLPKTSLIQSLVVATGAGFTTTSSLLSWLIYSLVTYPGVQDRLLQELIDNDITEDTQITADLTDR

LTFMDKLIKETQRRHNPSYQPARTAKVDMILPGGYKLPEESVVVGALHHLHNNPEVWSNPTRFDPDRWDTEEVKNRHKAA

YIPFATGPRMCIGFNFALQEVKVFLPKLVYRYKFTKENDGPIEYDPMFQLIRPNNLLSGSTGPWAYKA*

 

>CYP540B10  Aspergillus oryzae

           BAE59862.1

           80% TO 540B9

           revised 3/18/2009

MPIRQFYLLGEPVSSAREIEIESSLDIAGLQHLIAAHFAIVEPS (1)

GIGFQANDNTLNDIPEITSSNGPVAITIDGKE

VREPPGPKGLPIVGNFFEVYPDHLGNHQRLFEQYGPIIKTTSLGR

TTYQTNDPVLSSIVFAESDFFTKKINEAHPLYALKQPSAGVFLGDTETPEWKVAHKFLPPALGPKAVRHY

APTMQETVEDAFKVFDEFDKQGEAWNVYQYMLKLGSQAVGKLTLGLDFQHFTSPDANLHEMVNLIAELLS

LNKKVTSKGDWYAKLPFGDPQKLKQIKVRIIEMVEESIRSAERGGVVDLPLQDAALQASNMVDYAVRATD

NKGEKLPKTSLVWALTVATGAGFTTTSSLLSWLIYGLVTYPDMQERLLQELIDHDIDENTQLTADITDRL

TFLDKYIKETQRRHNPSFQPGRTAKVDLILPGGYKIPQDSVIIPALHHIHNNPELWDNPQKFNPDRWDTE

EVKNRHKAAYIPFAMGPRMCIGFNFALQEIKVFLPKLIYRYKFTREGDGHIEYDPMFQLIRPNNLYVRAE

RRVKWPPKTEN*

 

>CYP540B10  Aspergillus flavus

100% to CYP540B10  Aspergillus oryzae

80% to CYP540B7

AFL2G_06859

MPIRQFYLLGEPVSSAREIEIESSLDIAGLQHLIAAHFAIVEPSGIGFQANDNTLNDIPEITSSNGPVAITIDGKE

VREPPGPKGLPIVGNFFEVYPDHLGNHQRLFEQYGPIIKTTSLGRTTYQTNDPVLSSIVFAESDFFTKKINEAHPLYALKQPSA

GVFLGDTETPEWKVAHKFLPPALGPKAVRHYAPTMQETVEDAFKVFDEFDKQGEAWNVYQYMLKLGSQAVGKLTLGLDFQ

HFTSPDANLHEMVNLIAELLSLNKKVTSKGDWYAKLPFGDPQKLKQIKVRIIEMVEESIRSAERGGVVDLPLQDAALQAS

NMVDYAVRATDNKGEKLPKTSLVWALTVATGAGFTTTSSLLSWLIYGLVTYPDMQERLLQELIDHDIDENTQLTADITDR

LTFLDKYIKETQRRHNPSFQPGRTAKVDLILPGGYKIPQDSVIIPALHHIHNNPELWDNPQKFNPDRWDTEEVKNRHKAA

YIPFAMGPRMCIGFNFALQEIKVFLPKLIYRYKFTREGDGHIEYDPMFQLIRPNNLYVRAERR

VKWPPKTEN*

 

>CYP540B11  Neosartorya fischeri NRRL 181

           DS027697 join(1677302..1677440,1677496..1678332,1678385..1679094)

           73% to CYP540B4

MSTTTKLFHLLGEPISTAKEIQFESMTTYEDLRHLIAAHFAIVE

PNGIGFLSQDSILHDVPEIAASDDIISITIDGKAVREIPGPKGLPFIGNFFEVYPDHL

GNHQRLFEQYGPIFKTTNMGRTVYHTNDPQLSSIIFAETDFFTKKINAAHPLHPIKNQ

EAGVFLGDTDTPEWRTAHKFLPPALGPKAVRHYAPMMQETVEDAFNVFDALDEKEEAW

NVYQYMLKLGSQAVGKLVLGIDFKHFSSIDAPPHELVYRIAESLELNKKVTAWGDWYA

KLPFGDPQRLRNARGRIIDMVNESIQNAARGGIEDLPLQDAALKASNMVDYALRATDN

KGEKLPKTSLMQALVVATGAGFTTTSSLLSWLIYGLVTYPGVQERLLQELIDNGIDAD

TQLTADLTDRLTFMDKFIKETQRRHNPSYQPGRTAKVDMILPGGYKLPQDSVVIGALH

HLHNNPDVWSNPARFDPDRWDTEEVKNRHKTAYIPFATGPRMCIGFNFALQEVKVFLP

KLVYRYKFTRENDGHIEYDPMFQLIRPTNLYVRAERRVKWPPRSDKAAPSPSL

 

>CYP540B12 Aspergillus niger

fgenesh1_pg.C_scaffold_3000327|Aspni1

76% to CYP540B7

MPSQTFYLLGESPSSAKEIQIDPSATIDDLKHLIAAHFAIVEPSGIGFQGYDAALSELSEVLAAPGPLPLTIDGHAVREP

PCPRELPFVGTFFEVYPDHLGNHQRLFERYGPIVKTNNLGRVTYQTNDPKIAAIAFAESDFFTKKINEAHPLAGLKTPSA

GIFLGDTDTEEWRVAHKFLPPALGPKAVRHYAPTMQKTVEDSFKVFDALDEQGEAWNVYQYMLKLGSQAVGKLVLGLDFK

HFEAPDAPVHEMVHNIAEMLSLNKKVTSMGNWYAGLPFGDPKRLRSLRTSIENMVAESMEAASRGGHEDLPLQDAALKAD

NMVDYAIRATDNKGQKLPQSSLTWALVVATGAGFTTTSSLLSWLIYGLCVYPGMQERLLQELVDHGFDENTEITADFTDK

LEFLDKYIKETQRRHNPSFQPGRTAKVDLVLPGGYKLPKESVIVPALHHIHNHPDLWDNPARFNPDRWDTEEVKSRHKAA

YIPFAMGPRMCIGFNFALQEVKVFLPKLVYRYKFSMEDNGPIEYDPMFQLIRPNNLYVRAERRVRWPPKSRATAA*

 

>CYP540B13 Aspergillus niger

fgenesh1_pm.C_scaffold_1000635|Aspni1

81% to CYP540B11

MSAKLFHLLGEPVANAREIEVDSSLDYDGLRNLIAAHFAIVDPNGIGFQSEDAVFTSVAEIIASETPISIAIDGKPVREV

PGPKGLPIIGNFFEIYPDHLGNHQRLFEQYGPLFMTTNYGRTVYHTNDPELSAIVFSESDFFTKKINESHPLHAIKNKEA

GVFLGDTDTPEWRTAHKFLPPAFGPKAVRHYAPVMQQTVEDAFKVFDAFDEQGEAWNVYQYMLKLGSQAVGKLVLGIDFQ

HFANMNAPPHELILLIAESLELNKKITARGDWYAKLPFGDPKRLQQIRGRVWNIVDESIQNASRGGVEDLPLQDAALKAH

NMIDYAIRATDNKGEKLPKTSLIQALVVATGAGFTTTSSLLSWLIYSLVNYPGVQDRLLQELVDNGIDADTQITADLTDR

LTFMDKFIKETQRRHNPSYQPARTAKVDMILPGGYKLPEDSVVIGALHHLHNNPEVWDNPTRFNPDRWDTEEVKNRHKSS

YIPFAAGPRMCIGFNFALQEVKVFLPKLVYRYRFTKETDGPIEYDPMFQLIRPNNLYVRAERRVKWPPKTESPLPTASL*

 

>CYP540B14  Mgf Mycosphaerella graminicola

56% to 540B11, 57% to 540B4

MATTKSFYFQGSKDKNLSLDVDVENINDLAGLKEVIGTSCAVVKPEGLSFYLEDELLEEISDIKEAQGRISIKVDGHTVR

SVPGPPGLPLIGNYFEVFPDHLGNNQRLFDKYGPIFSTNNMGSVVCQTNDPELADVCFAETEFFSKEINSSHPLYPIKQQ

QAGVFLADTSAPAWKVVHKFLPPALGPKAVRHYAPEMNGCCNEAFPVFDKLESENEAWNVYQFMLKLSSGAIGKIMLGQD

FEHFSSVDAPLHKMVLAMAEVLSINKKIASRGEWYSHLPFGDPARLKNLQKYMAEFIETSIKNAKSNGTEDLPLQDAALK

ASNVIDYLVRATDSTGKQLPKENLMPAVTVACGAGFTTTSSLLSWCIYALVTYPGIQAQLLQELVDNDINDSDNITAEQI

DELPVLANFIKEAQRRHNPSYQPGRTAQKDLILPGGYQMKKGTVVICAIHHIHNNPKNWDNPDKFDPDRWNHDPLKGKPK

SAYAPFAQGGRMCVGFNFALLEVKIFMCKLVYRYHWDKEGDMETSYDPFFQLIRPVDLYVKTTKRQTYPTKSGEGQA

 

>CYP540B15 Fusarium oxysporum

90% to CYP540B5

FOXG_04494 revised

MATKRFHLMGEDPSTAQEIEIPTSLDEQGLQHLVASHFAIVDPS

GIGFVTESDALTTVADVLATDDISITIDGKAVREVPGPKGLPLVGNYFEVY

PDHLGNHQRLFEKYGPLFKTTNMGSTIYHTNDPKLANIVFGETDFFSKRIIEGHPLFPIKNKEAGVFLGDTDTEEWKEVH

KFLPPALGPKAVRHYAPTMQKTVEDAFKVFDELDERDEAWNVYPYMLKLGSQAVGKLVLGMDFQHFTSPDARPHAMVMRI

AQSLELNKKITSMGSWYKNLPFGDPQRLRDARAHMELMMKESVENASKGEGDLELQDAAVQAENMVDYVLRASDSKGNKL

PRERIMEPLVVATGAGFTTTSSLLSWLLYGLVAYPGMQERLLQEMVDNGFDENTQITADLTNKLTFLDKYIKETQRRHNP

SYQPARTASVDMVLPGGYKLPKDSIVIPALHHIHNNTKIWDNPALFDPDRWDTDRVKNRPNASYIPFATGPRMCVGFNFA

LQEVKVFLPKLVYRYKFSLAQDGPVEYDPMFQLIRPNNLYVRAERRIKWPSKSD*

 

>CYP540B15 Fusarium verticillioides

99% to CYP540B15 Fusarium oxysporum = ortholog, only 3 aa diffs

FVEG_07621 revised

MATKRFHLMGEDPSTAQEIEIPTSLDEQGLQHLVASHFAIVDPS

GIGFVTESDALTTVADVLAADDISITIDGKAVREVPGPKGLPLVGNYFEV

YPDHLGNHQRLFEKYGPLFKTTNMGSTIYHTNDPKLANIVF

GETDFFSKRIIEGHPLFPIKNKEAGVFLGDTDTEEWKEVHKFLPPAL

GPKAVRHYAPTMQKTVEDAFKVFDELDERDEAWNVYPYMLKLGSQAVGKLVLG

MDFQHFTSPDARPHAMVMRIAQSLELNKKITSMGSWYKNLPFGDPQRLRDAR

AHMELMMKESVENASKGEGDLELQDAAVQAENMVDYVLRASDSKGNKL

SRERIMEPLVVATGAGFTTTSSLLSWLLYGLVAYPGMQERLLQEMVDNGF

DENTQITADLTNKLTFLDKYIKETQRRHNPSYQPARTASVDMILPGGYKL

PKDSIVIPALHHIHNNTKIWDNPALFDPDRWDTDRVKNRPNASYIPFATG

PRMCVGFNFALQEVKVFLPKLVYRYKFSLAQDGPVEYDPMFQLIRPNNLY

VRAERRIKWPSKSD*

 

>CYP540B16 Fusarium oxysporum

92% to CYP540B3

FOXG_16987

MATKQFYLLGEAPSTTREVDLPPAVDFEELQNIVASHFAIVKPNGVGFVHDDRRLNVVSEVLDKDEPIAVSINGNAVRDV

PGPAGIPYFGNYLEIYPDHLGNHQRLFEKYGPLFVTNSMGNRLYQTNSAELSNIFLAEDHYFTKDIVPGHPLHPIKNQEA

GVFLADTDTEQWRLAHKFLPPALGPKAVRHYAPTMQRTVEQSFKVFDELDEKGEAWNVYQYMLKLGSQAVGKLVLGMDFA

HFEEVDSPLHEMVLKIAENLELNKRVSSMGAWYAKMPFGDPKKVRDTMGRIMEMMTESIARASKGQEDLELQDAALKAEN

VVDYFLRAKDNKGSKLPPSQFAPTLLVATAAGFTTTSSLLSWLIYSLVKYPGNQERLLQELIDNDWDEDTQVTAETTSKL

SFLDKFIKETQRLHNPSFQPGRTAKVDMILPGGYRLPKGAVVISALHHMHNNKDIWENPGRFDPDRWDTEQVKNRPPGSY

IPFATGPRMCVGFNFALQEIKVFLPKLVYRYKFSLAQDGPIEYDPYFQLIRPNNLYVQAEKRVKWPPKSE*

 

>CYP540B16 Fusarium verticillioides

96% to CYP540B16

FVEG_13789

MSTKHFYLLGEAPSTAREIELPPAVDFEELQNIVASHFAIVKPNGVGFVHD

DKRLNAVSEVLDKDELIAVSVNGNAVRDVPGPAGIPYFGNYLEIYPDHL

GNHQRLFEKYGPLFVTNSMGNRLYQTNSAELSNIFLAEDHYFTKDIVPGHP

LHPIKNQEAGVFLADTDTEQWRLAHKFLPPALGPKAVRHYAPTMQRTVE

QSYKVFDELDEKGEAWNVYQYMLKLGSQAVGKLVLGMDFAHFEEVDAPL

HEMVLKIAENLELNKKVSSMGAWYAKMPFGDPKKVRDTTARIMEMMMESIA

RASKGKEDLELQDAALKAENVVDYFLRAKDNKGSKLPPSQFAPTLLVATAAG

FTTTSSLLSWLIYSLVKYPGNQERLLQELIDNDWDDDTQVTAETTNKL

SFLDKFIKETQRLHNPSFQPARTAKVDMILPGGYRLPKGAVVISALHHMH

NNKDVWENPGRFDPDRWDTEQVKNRPPGSYIPFATGPRMCVGFNFALQEI

KVFLPKLVYRYKFSLAQDGPIEYDPYFQLIRPNNLYIQAEKRVKWPPKSE*

 

>CYP540B17 Aspergillus clavatus

85% to CYP540B7

ACLA_013210

MPTQQFYLSGEPISSARTIELDGMQSLDDLRNLIAAHFAIVEPSGIDFQGRDTCLTDIPEITSSASPVAITVDGHAVRDV

PSPAPLPLVGNFFQVFPDHLGNHQRLFEQFGPLIKTTTLGRTVYQTNDPAISAIVLNESDFFTKKINEAHPLYSLRTPVA

GIFLGDTDTPEWRVAHKFIPPALGPKAVRHYAPTMQKTVEDACQVFDELDERGEAWNVYQFMLKLGSQAVGKLTLGLDFQ

HFTDVDAPIHEMVHAIAEVLSLNKKVTSKGDWYANLPFGDPKRLKMLKARIEQMVEESIENAGRGGVEDLPLQDAALRAS

NMVDYAIRATDNKGEKLPKSSLIWALVVAAAAGFTTTSSLLSWLIYGLVTYPGMQDRLLQELIDHGFTDDTEITADFTDQ

LPFLDKYIKETQRRHNPSFQPGRTAKVDLVLPGGYRIPKDAVVIPALHHIHNNPAVWDNPARFDPDRWDTDEVKKRHKAA

YIPFPTGPRMCIGFNFALQEVKVFLPKLIYKYKFSREGDGPIEYDPMFQLIRPNNLYVRAEKRVKWPPKSE*

 

>CYP540B18 Trichoderma atroviride

GE290941.1

77% to CYP540B10

MAIQQFYLLGESETATTAIDIDSNLDFDGLRHLVASYFAIVEPNGVGFQFQENALQEVSDVSSAAGPVA

ISIDGHAVREVPGPKG

LPYLGNFLEIYPDHLGNHQRLFEHYGPIIKTVNMGRAVYQTNDPKIATIAFAESDFFTK &

KINEDHPLYALKTPAAGVFLG &

DTETEEWKIAHKFLPP

 

>CYP540B19 Aspergillus terreus

83% to CYP540B12

ATEG_10264.1

MPSQTFYLLGQSLSSAKEVGIETSQTFESLQFLIAAHFAIVDPSGITFQGPDAVLTEVAEVLAATAPVPITIDGHPVRDP

PCPKELPFVGTFFEVYPDHLGNHQRLFERYGPVIKTSNLGRVTYQTNDPKIAAIAFAESDFFTKKINESHPLHALKTPAA

GVFLGDTDTPEWRVAHKFLPPALGPKAVRHYAPTMQQTVEDAFKVFDKLDEQSEAWNVYQYMLKLGSQAVGKLTLGMDFQ

HFDAPDTPVHEMVHNIAEVLSLNKKVTSKGDWYSALPFGDPRRLKNLRVRIEEMVGESMKKATRAGIEDLPLQDAALTAS

NMVDYAVRATDNKGEKLPKSSVVWALIVATGAGFTTTSSLLSWLIYGLCEYKGMQERLLQELIDHGFDENTKMTADFTEK

LEFLDKYIKETQRRHNPSFQPGRTAKVDLILPGGYKLPKDAVIIPALHHIHNNPDLWDNPARFNPDRWDTEEVKSRHKAA

YIPFAMGPRMCIGFNFALQEIKVFLPKLIYRYKFSREGDGPIEYDPMFQLIRPNNLYVRAERRTKWPVKSRSASP*

 

>CYP540C1 Magnaporthe grisea MG11098.4  46% to CYP540A1 missing N-term

AACU01001916.1 cont2.2255 runs off end

2 IHRVPGLDAFPIVGNRLELYPDILGNVERLCARYGGIIKTIN

MGSVTYYTNDPSIARHVLRESEFFTKKTSDPAHPLHFMADQEALFTCDSD

SPAFGPSHRFIPPCMAPKAIRHYTPSVQQCVQKVLPVLDAISDSSLAFNV

YQYMFKLAGQVIWKIVFGADLGHFDSLDAKPFETIRDLGQYVALMKRVSL

RGQWYGYLPFGDPRRMREARDRVWKGIGEAIEQAARTGTDNRDLPLQEAA

LHAACLADYLQRAVDNEGNKLPREYLVSNCVILFGAG

FLTSSSLLAWCIFALCRYEG

VQDRLLQELVDHGARPDVVWTMDQLNAMPYLDQFVKETQRMHN

PSFQTARNARRDVVLPGGKAIPAGSVVIPMFPALHKNPAHWDNPARFDPE

RWGTPAVKKLPRAAYTPFAAGSRGCIGFSLALLEVKMAMAILVYRYKFSD

ASLEPVVYDPEFLVVRPLNFYAKAAIRTVLPVARGEGAGAY*

 

>CYP540C2 Magnaporthe grisea MG09598.4  poor match 62% to part of MG11098.4

AACU01001656.1 cont2.1833

14398 MFISNYIVDETVNRSLLKHGSRVITVGTGSVTSERSLNTCLLSSQRPRYI

      SLENITDLEALKSRVGEEFGVVQPS

14093 EITFHAGDGTQLHCLGDVSRCRSTI

DIRIAKIPIHSPLACSRDELYPNLFGNVDRLCARYGAKVIKTDN

MGMVAY

YSNDPVIARHVLQEGGFFSKRTPDPGDPLHFVSDSEALLGDGDSPAFGPS

HHFIPPCVAPKAVRYYTPSVQRSVEKAFYILDAMSDGSLAFNVYQYMFKL

AGQIVWKTVFGADLGHFNSIEAKSYKTIHHLSEYLALKQRLSLRPPWYAN

LPFGDPRRMREVRHLLWAGVDEAIEQCCTDACTDEKESQLYKGALHTNCL

AADLQKAVDGKGNRLPREHLVNSCLVLFGAGFVTLSTLLSWCIYALCRYNS

VQDRLLKELLDHGVAEDKA

WTMDELNDMPYLDWFVKETQRIYSPSLQTARVAQRDVVLPGGVMISEGSV

VIPTLSHQDSVSRDSPVRFDPESWETKAARTLPQXXXXXXXXXSRGCAGFSRTLLEVKM

AMALLVYRYEFSHATVEPVVYDAELLAVRPVNLYARTKRRTCWPGPSPKK

EDSF*

 

>CYP540D1 Aspergillus nidulans AN9218.1 46% to 540B3 505 clan

MTVQTFYLIGEKERSTRELDVGDPKTVNALRQGLAEVFNILSAEGIDFHD

CHGPISTIESILRSESVGITVNGHPVRYPQQPQGIPIFGNHFEIYPDHLG

NHERLFNKYGSVIRTNNMGRVTYLTNDPDIAALAFRDNDYFTKAPSSASH

PLYGIRDQTALFLCDTESPAWKEAHKFIPPSMTPRAVRHYTPLLQQSVDT

VFNVLDKFDNNGQAFNVYHLTAKLASQVICQLVLGVDLHHFDAVDSPVHP

IIVLLQRYLTLNRRVQTKGAWYSYLPF GDPVALKNTRRELYGLIEEAVIT

CQKKNGGTTGDLPIQT AALHATCLVDYLARATDEHGNKLRHEYILSNTLA

LVGAGFVTSSAFLSWLIYSLVEYPGQQDRLLQELVDHGAVSDKRWTYDEI

QALPFLDAFVKEAQRMHSPSFQPARNVKKDIILPGGWALPQGSILIPSIP

HLHHHTAYWENPDRFDPDRWRTEKVKNRHRSVYVPFAAGPRSCIGFNVAL

QEVKISLAELVYRYEFVNATNEGIEYDPDFIVIRPVNFYVRAIRRTEWPA

RSP*

 

>CYP540E1 Aspergillus clavatus

41% to CYP540D1, 42% to CYP540A3, one frameshift

potential pseudogene

AAKD03000007.1

ACLA_003960

MATKSLYLEGRPPEETLCSVDISGLKTLDDLKYRTASIFGVALPKSISFHNESGLLSSMDDISTTVTKIGVHVDDSVIRV

PHGPRELPFVGQYFEIHPDHLGNHERLFQKYGNVIKTVVMGRTVYLTNDPRVTEVVFGENEFFTKKTSDFNHPFFWVNDN

MALLVGDTGAPAVKLAHKFIPPSMSTAAAQHYMPILQHAIQDSFAVFDQLDDRQAAWNTFQFMFKLSGQMIGKVALGMDL

DHFQTVDTQPHQIMHLLGESIRLTRQASLSHPYLVYLPFSA

VRCLRNTKKT &

LVSALYQTVSPSLISEAPDLPMAQ

AAIQSTCIADYLKRAVDEDNQKMPKEYILPNLVSILAAG

LSTTSSVGSYLLYLLAHHQDMQSRILQELINNNVTPTASWDYKTLMSLPFLDSFVKETLRLHNPAFQPARYAKKDVIVPG

AYHLPAGAVVNPVFFSLHRNKDYWENAHRFDPDRWLAADVQKHRTAYTPFAVGPRRCVGFNLANLQLRLMLAMLIFRYKF

VDVSPEPVVNDHEYLLDRLVNCYVRAVRRTSWPEKEL*

 

>CYP541A1 N. crassa AABX01000779.1 cont3.283 NCU05006.1 (version3)

7113 MSTPIPRPPGIPVLGNVFDITPSNTWWSLKALAEKY (1) 7220

7273 GEIFQVKILGKTIV

FVASAALAEEICDEQRFQKYVGGPIVEIRATVHDSLFTAFHHEQSWGIHH

RIIAPLLTPQAVSGYTDDINLCATEVIQKWASLGDSNVLEPLVDLNLLNL

EATSLTLFSQKLNCIQAGGHPVIQAMEDAVSEAIMRPTRPGLVNWLLYSS

KFKKATKTLRTWAADTVKYRQENPTDKHDMLWAFMNAKDPETGKGLSESE

ILDEIVTMPIGSATAPCAVTATIYYLLQNPEVVTKAREELDRVIGTGPLK

DEHLSQLHYIEGITRETLRLSCAAPGFNIEPIPRQNKADKSPLLLQGGKY

QVAHDQKLIIVLAGVNRDPAVFEDSLAFKPERMMGEEFDRLPKGVKKWYG

NGKRECIGKEWANNFLKIVTARLIHEIDFEVADEGYEFRQDGWFQIRPVA

FKVRVKPRVRA* 8550

 

>CYP541A2 AAIL01000415.1 Trichoderma reesei QM9414

62% to CYP541A1, no introns

66598  MTTPIPKPPGVPLLGNIFDVKPGNTWDSLRKLAEKYGEIFQIKVLGQTIVFVASAALAEE  66777

66778  LCDEKRFRKYVGGPVVEIRAAVHDSLFTAYDHEPSWGVAHRIIYPKLSPEAMKLHFGEMR  66957

66958  DLATELFNTWRGLGDKNTISPLEQLNRLNLEATTYVLYGKRLNTLIGPPHPELQAMEDST  67137

67138  SEAMKRPTRPGVLNSLLYGRKFKSSIKVMRDYAADLVKYRQENPTDRQDVLAAIMNTPDP  67317

67318  ETGKKLTDSQVIDEIVTMPIGSSTAPCLLVWAIYFLLKNPETIVKAREELDRVLGDREFE  67497

67498  LEHLEQLEYVQGIVRESLRLSSAAPGFNIEPLPKEGDKSPVLLGGGKYQVAHNQAMII  67671

67672  VLHGVNRDPAVFDDPLAFKPERMVGEAFENLPAAAKKWFGNGKRVCIGRYYALQWSLVVL  67851

67852  AKMIREFDFEMADPEYELALDGWFNIRPIDFYVKVKPRAT*  67974

 

>CYP541A3 AAFU01000004.1 Chaetomium globosum CBS 148.51

66% to 541A1

89914  MSTPIPQPPGVPLLGNIFDVDPSNTWWSLKTLAEKY (1)

89700  GEIFQIKVLGHRIVFVASAALAEEICDEKRFRKYVGGPIVEIRYTVHDALFTAYDHEESW  89521

89520  GIAHRIMVPQLTPDAVASHFDEVIQCTDGLIAKWSGLESGGRTRPMDDLNRLNLEANTLT  89341

89340  LFGKKLNCFEGPEHPMIKAMEDATSEAMKRPNRPKLLNWLIHDGKLKKATKVMRGFAADL  89161

89160  VKERQENPSERRDMLWTMLNVKDPETGKALTESQVIDEIVSMPIGSSTAPCAIAAAIYFL  88981

88980  LQNPDALVKARDEIDAVVGEGPFKQEHVSQLQYVAGLVRETLRLSCAAPGFNIEPIPK--  88807

88806  KGDESPVLLAGGKYQVPHNQAMIIVLAGVNRDPAVFEEPLAFRPERMMGEAYEQLPSAVK  88627

88626  KWFGNGKRECIGKHWAWEFLVVVITKLISELDFEAADPNYQFKQDGWFNIRPIDFSVKVK  88447

88446  SRTR  88435

 

>CYP541B1 Aspergillus nidulans AN8004.1 49% to 541A1 505 clan

MPTPIPQPKGLPVLGNLFSLDSKNPWGSFNKLAAASPSNRPIFKISILGH

DIVFVTSAALLEEICDETRFRKCVTGPIVQIRDAVHDSLFTAYHSESEIW

GVAHRIMKPLVSPEAIEGVFNEMRETTDDLIKKWTASSKQRVNVCNDLDR

SNHEANMLAFFHQKVSIMNGTEPAVIASCQNATMEAVKRSSRPKFLTWLM

GHQKRYDSDIKIMRNYGAEIVRKRREEYPDGGPKDMLHALLHGVDSETGK

KLSESQVLDEIINIFIGSATAPNLLSFGLYYLATNPDVVTKAREELDAVV

GSGPFEHAHLSQIPYTEGILRESLRLSATAPGFNIEPIPSPGNEPVLLAG

GEYQVPRTQPLIALLTAVNRDPEVFEDPESFKPERMVGEKYDALPSGVKK

GFGNGKRQCFGTRYAWEWSLLVLARLIKEVDFELADKKYKVTNEGVNYNG

AFSTRPVGFFLLASPRASV*

 

>CYP541B2  XM_742988.1 Aspergillus fumigatus

67% to 541B1 49% to 541A1, 98% to CYP541B4Nf allele

MPTPIPLPSGLPILGNLLSISPGNTWGSFNKLAVNNKNFYPIFK

ITILGHDIVFITSAKLLEEICDEKRFRKCVTGPIVEIREAVHDSLFTAYHDEESWGIA

HRIMAPLMTPEAVIDAFAGMRETATDLVKKWTAGPRQRINVSNDMDRLNHAANMLCFF

DQRIHCLEGPEPSVLQAMEAATFEALRRPTRPKVLKWLIYQRRFDRYIKTMRDYAAEI

VAKRRAAPTEKKDMLHAIMHGTDPQTGKALTESQYLDEIINLFIGSATAANLVSFALY

YLMKNPHEIARAREEIDAVVGGPAAQLEHEHLARLPYCEAILRESLRLSATAPGFNIE

PIPDLEQPVLLAGGEYQVPNKQPLIALLAAVNRDPEVFEDPDAFKPERMVGEKYDRLP

SGVKKGFGNGKRECFGKRYAWEWSFMILVTIMKDVDFVLADKNYKTEVGGVNYNGAFS

TKPLGLFALTGPRQSV*

 

>CYP541B2 AAKE02000015.1 Neosartorya fischeri NRRL 181

67% to 541B1, 97% to CYP541B2 Aspergillus fumigatus = ortholog

NFIA_039470

347217  MPTPIPHPSGLPILGNLFSISPGNTWGSFNKLAVNNKNF (1)  347101

347032  YPIFKITILGHDIVFITSAKLLEEICDEKRFRKCVTGPIVEIREAVHDSLFTAYHDEESWGI  346847

346846  AHRIMAPLVTPEAVIDAFAGMRETATDLVKKWTAGPRQRINVSNDMDRLNHAANMLCFF  346670

346669  DQRIHCLEGPEPPVLQAMEAATFEALRRPTRPKVLKWLIYQRRFDSYIKTMRDYAAEIVA  346490

346489  KRRAAPTEKKDMLHAIMHGTDPQTGKALTESQHLDEIINIFIGSATSANLVSFALYYLMK  346310

346309  NPHEIARAREEIDAVVGGPTAQLEHEHLARLPYCEAILRESLRLSATAPGFNIEPIPDL  346133

346132  EQPVLLAGGEYQVPNKQPLIALLAAVNRDPEVFEDPDAFKPERMVGEKYDRLPAGVK  345962

345961  KGFGNGKRECFGKRYAWEWSFMILVTIMKDVDFVLADKNYKTEVGGVNYNGAFSTKPLGL  345782

345781  FALTGPRQSV*  345749

 

>CYP541B3  AP007162 Aspergillus oryzae

50% to 541A1, 66% to 541B1

MPSPIPKPKGVPILGNVFDLDGNNTWGSFNKLAKDYRPIFKINI

LGKDIVFITGAHLLEEVCDETRFRKCVAGPILAIRDAVNDSLFTAKHTTEEIISWGIA

HRIMAPLVSWEAVEQVFSGIQETTDDLIKKWTTGSRQRINVTNDLDRCNHAANMLCFF

DQRVHIMEGPEPAVIKAMGCATNEAMHRPFRLRIVNWLFHSRKWNAWIKTMRDYGKDI

VETRRANPTDKKDMLYALLEGKDPETGKGLTDSQVLDEIINIFIGSATAPNLIAFAMY

YLANNPDAAAKAREELDAVVGGPSVRIEHEHLARLPYTEGVLRESFRLSGVAPGFNIE

PIPENEGPVLLAGGEYEVPKGQALIAILAAVNRDPAVFEDPEAFKPERMVGENFDRLP

KGVKKGFGNGKRECIGKRYAWQWSFMVLASILKDVEFELADPNYKVGNDGVNCNGAFS

VRPQEMFVVTGPRARAA*

 

>CYP541B3 # AAIH01000171.1 Aspergillus flavus NRRL3357

the ortholog of Aspergillus oryzae CYP541B3 only 2 aa diffs

AFL2G_09797

MPSPIPKPKGVPILGNVFDLDGNNTWGSFNKLAKDYRPIFKINILGKDIVFITGAHLLEEVCDETRFRKCVAGPILAIRD

AVNDSLFTAKHTPEEIISWGIAHRIMAPLVSWEAVEQVFSGIQETTDDLIKKWTTGSRQRINVTNDLDRCNHAANMLCFF

DQRVHIMEGPEPAVIKAMDCATNEAMHRPFRLRIVNWLFHSRKWNAWIKTMRDYGKDIVETRRANPTDKKDMLYALLEGK

DPETGKGLTDSQVLDEIINIFIGSATAPNLIAFAMYYLANNPDAAAKAREELDAVVGGPSVRIEHEHLARLPYTEGVLRE

SFRLSGVAPGFNIEPIPENEGPVLLAGGEYEVPKGQALIAILAAVNRDPAVFEDPEAFKPERMVGENFDRLPKGVKKGFG

NGKRECIGKRYAWQWSFMVLASILKDVEFELADPNYKVGNDGVNCNGAFSVRPQEMFVVTGPRARAA*

 

>CYP541B4 AAKD02000007.1 Aspergillus clavatus NRRL 1

note only 215bp from CYP661B2

66% to 541B1, 79% to CYP541B2

ACLA_002550

578942  MPTPIPQPPGLPILGNLFDLKPGNTWGSFNKLAVKY  (1) 578835

578761  RPIFKISVVGHDIVFVTSAKLLEEICDEKRFRKCVTGPIVEIRQAVHDSLFTAYHNEESWG  578579

578578  IAHRIMAPLVSPDAVAEAFAGMRETTKDLIQKWTAGPRQRVNVCNDLDRLNHAANMLCFF  578399

578398  DQRIHCLEGQEPSVIKAMDAATNEAMRRAARPKLLNWLIYQRRFNAYNRTMRDYAAEIVA  578219

578218  QRRAYPTDKKDLLDALLHGKDPQTGLALTDSQHLDEIINIFIGSATSPNLVSFALYYLMK  578039

578038  HPQEITRAREEIDGVVIDQLEHRHLASLPYCEAILRESLRLCATAPGFNIEPIPGQE  577868

577867  EPVLLAGGEYQIPNKQPMIALLSAVNRDPEVFEEPDAFIPERMVGENYERLPAGVKKGFG  577688

577687  NGKRQCFGTRFAWEWCFMVLVTILREVNFDLADKQYQTAVEGINYNGAFSTKPLGLFAVT  577508

577507  GPRQRL*  577487

 

>CYP541B5 AAJN01000228.1 Aspergillus terreus NIH2624

63% to 541B1

ATEG_09231.1

134658  MPSPIPQPKGYPLVGNLFDIDSQNPWGSFNKLALKNKY (1)

134844  RPIFKINILGKDLVFVTGAALLEEVCDEKRFRKCVSGPIVQIRDAVHCSLFTAYEKEM  135017

135018  EDWGIAHRIMAPMVSTEAVAELYSGMKETMPDLIKKWTAGPRQRVNVTNDLDRLNHAANM  135197

135198  LCFFNQRVDCVNGPEPPAIHAHEESTVEATRRSSRMKLVNWLFHQKKYDNYIKIMRDYGA  135377

135378  DIIAHRKAYPTDKNDMLNALMNGKDPQTGKGLTESQVQDEIINCFIGSATAPNLISFALY  135557

135558  YLMKNPDTIRQAREEIDAVVGVSESVEQEHLARLPYCKAIIDETFRLSAVAPGFNLEPH  135734

135735  PDDEGPVLLAGGEYEIPRKQALIVLLSAVGRDPAVFEDPDAFKPERMLGEKFDRLPAGVK  135914

135915  KRFGNGKRECIGKRYAYEWSFMTLVSILKDVEFELADANYVMNNDGVNYNGAFSVKPQGF  136094

136095  FAVTGPRQRSGH*  136133

 

>CYP541B6 AAJI01002279.1 Ajellomyces capsulatus NAm1

98% to Histoplasma capsulatum G217B

HCB04326.1

55% to 541B1, 47% to 541A1

98% to HCB08024.1 Histoplasma capsulatum G217B (anamorph)

10797  MTTPIPGPPGVPLLGNIFDINPKDTWGSLKSLADKY (1)  10904

10960  EIYKVNALGHQIVFIGSAALLEEICDETRFRKCVTGPVVEIRRAVHDSLFTAYHHEKSWDV  11142

11143  AHRIMAPLVSPSAAEAMFTQMVECAATLVSRWTSDPGQRIDVTSDLQRSDLQSVLACYFN  11322

11323  QAANYFGGPEPSMIAAMQNSTLEAMKRPNRPKLLTRLLYQRKFDKDIKTMRSFAAEIIAN  11502

11503  RKSESAPKSDMLHALLNNPDPETGEVLGEERVIDEIITIFIGSATAPCLVSFAVYYLLQN  11682

11683  PEEIVKARDEIDSILGANGEITSSNLSSFAYCEAILREAFRLSAVAPAFNIEPIPSDEP  11859

11860  DEVQLAGGKYQISRKQPMIAVLSAVNRDPAVFDEPEAFKPERMLGEAYDKLPSGVKKGFG  12039

12040  NGKRRCFGKVPAWQWCLITLISILRKVDLTLANKDYKLASNGAFCVRPLEFFALA GPRER*  12222

 

>CYP541B7 Aspergillus niger

estExt_GeneWisePlus.C_21802|Aspni1

67% to CYP541B3v1

MPSPIPKPPGVFLLGNVKDVDAANPWNSFNKLAAKYRPICKVTILGVDIVLITGAALLEEICDETRFQKCVAGPIIEIRE

AVHSSLFTAKINDKEMSQWGVAHRIMAPLLTPGAVDQVFADMREVSTDLIKKWTAGPRQRINVTNDLDRLNHAANMLCFF

DQRLHCLEGPEPSLIKAMENATTEAVRRSSRLKLVNWLFYQRKFNAYNKTLRDYSASCMDYRRAHPSAKKDMLYALMEGK

DPETGEGLNDEQIVDEIINVFIGSATAPNLVAFGLYYLMKNPHVIKRAREEIDAVVGGPGAKIEHEHLSRLPYCLAIAQE

TMRLAAPAPGFNREAIPNQEGPVLLAGGEYEVPRKQALIAVLYGVNRDPAVFEDPDAFKPERMMGEAYERLPSAVKKGFG

TGKRVCIGKKYAWEWSYFTLVSILKDVEFELADKDYVTDGAGNNYNGAFTVKPLDFSAITGPRPRAG*

 

>CYP542A1 N. crassa AABX01000017.1 cont3.568 NCU09103.1 EST = AA902060

114647 MASTTAFLPRLTTITFSFLTQPLLIMFLLSLLTLYTIYI

       AYHRLYLSPLADIPGPRLAALTQWYEFYYEVILHGQYTF

       KIIELHKQYGPIIRINPWEVHIADPDFHRELLPTNTNRR

       RHRTPFFTKQFGAD (1) 114255

114135 ESIVATNDHDLHKLRRSAVGPFFSTQNTRALQPVIEERV

       DALLARLREHGKTKKDIPLNMMYAYSATSY (1) 113929

113861 DIISEYCFAKSEHA (1) 113820

113745 VEDPDFRAEITNGILTGSNYGKIFQHFPFLVPFLASIP

       PGMLAAISPFYRTFLHLRACITAQIGEIEKSLRSEEGK

       NAHLDIPHPTIFHSFVNTEALPPIEKSVPRIAQEGQV

       LVQAGTVTTSWALTISTFHLLDQHATALAKLRK

       *LREALPDDADEPVELAR

       LEQLPYLRAVIKESMRLSVGASGRITRVAPDETLRFK

       PSKLCLRCYPDDQLKTTAEDKEKEWLLPPGTEVSMTSY

       QITTNPEIFPDPHAFVPERWLGKENEMRLDKYMT

       VFGHGARVCLGMQLAYAEMYLMLSKMWRVWEGGPQVGG

       GEDEDGKEDVEEKKGRRDGRTTVGRLRLAEGVTVR

       DAEMAEDWFIPVPYRGSKGVRVYFESY* 112624

 

>CYP542B1 old name = CYP580A1 Fusarium graminearum FG08809.1 AACM01000355 FGcontig1.355_scaffold5

MDSNTIMAVQETIAARNWQQTGCFFAGVWLVY

IVALVVWRLWFSPLAHIPGPKLAALTQYYEFYYDFVLGGQYNYKIIDMHERYG

PIVRINPWEVHVGDPEFFSDLYT

SPSPSRRRDKWTFYTQQ(0)

SGAPRTQVPHPNALAAPKTATEGSLAAIDHGLNKLRRSALI

PLYTTQTVRELQPVIEERVEALLTAFMNYAEVSNGQPLDVMYPYSALTNDVINEYAFA

RSDHLVEKPDFGAEVTNDLLIGTHMGPCVQQLDWVLTLVNALPESISNRCMPGWGGFL

KMKNDIFEQIHSVDPPQSTGKWRMVGGHPTMLSTRARSTREKNASRQAQESQISVQGG

ALTTSWAMSLATFHLLNRPETLRKLRDELFAAMPDAYETVPLSRLEKLPYLRGVVKEA

LRLGIGTSSRLARVAPDEALVYHDRENGEDWNLPPGSIVGMSPYKTVMDESIFYDARG

FHPERWVEDGERLDKYLDIFCSGSRICLGMALAHAELYLTLAKLFRRWGSGGVVFGSD

DGDQRYGDVGYLSIFETRVRDCEIDADYFMPIPYKGSEGFRFSFETY

 

>CYP542B2 Nectria haematococca

e_gw1.1.1096.1

Necha1/scaffold_1:865645-867387

85% to CYP542B1 (old 580A1), 44% to CYP542A1

MAVPEIIASLTWQQTGCLLAGVWLIYLIQLVIQRLWLSPLAHIPGPKLAALTQYYEFYYDFVLGGQYTFK

IIDMHEKYGPIVRINPWEVHVGDPDFFSELYTGPSRRRDKWTFYTQQ (0)

SGAPRTRVPHPNALTGPNTATEG

SLAAIDHSLHKLRRSALNPFSSTQTVRKLQPVIEERVDALLDAFLNYAEVSNGQPLDVMYPYSAFTN (1)

DVINEYAFA

RSDHLVEKSDFGAEVTNDLLIGTHMGPCVQHLDWVLTLVNALPESISNRCMPGWGGFLKMKNDILEHIHS

IDPPQSTGKWTMDGGHPTMLSRPRSMRVKQSPQVQEGQILVQGGTLTTSWAMSLATFHLLNRPETLRKLR

DELFAAIPDAHDTTPLSRLEKLPYLGGVVKEALRLGIGTSSRLARVAADETLVYHDHVNNTVWRLPPGSI

VGMSPYKTVMDENIFYDARGFHPERWVEDGERLDKYLDIFCSGTRVCLGMALAQAELYLMLAKIFRRWGS

GGVVFGSDDGDQRFGDVGYLSIYETRVRDCEPAADFFMPIPYK (0)

GSEGLRFVFEAY*

 

>CYP542B3 Fusarium oxysporum

91% to CYP542B1 Fusarium graminearum

FOXG_03513

MASNTFMAVQETIAALTWQQTGCLLAGIWLIYLVQLVIWRLWLSPLAHIPGPKLAALTQYYEFYYDFVLGGQYTYKIIDM

HERYGPIVRINPWE VHVGDPEFFSDLYTGPSRRRDKWTFYTQQ SGAPRTRVPHPTALAGPKTATEGSLAAIDHSLHKLRR

SALNPFYSTQTVRELQPVIEERVEALLGAFLNYAEVSNGQPLDVMYPYSAFTNDVINEYAFARSDHLVEKPDFGAEVTND

LLIGTHMGPCVQQLDWVLTLVNALPESISNRCMPGWGGFLKMKNDILEQIHSVDPPQSSGKWMMDGGHPTMLSTKTRSVR

DKTASRQTQESQITVQGGTLTTSWAMSLATFHLLNRPETLRKLRDELFDAIPDAYETIPLSKLEKLPYLRGVVKEALRLG

IGTSSRLARVAPDEALVYHDRENDEEWNLPPGSIVGMSPYKTVMDENIFYDARGFHPERWVEDGERLDKYLDIFCSGSRI

CLGMALAHAELYLTLAKLFRRWGSGGVVYGSDDGDQRFGDVGYLSIFETRVRDCEIDADYFMPIPYKGSEGFRFVFETY

 

>CYP542B3 Fusarium verticillioides

98% to CYP542B3 Fusarium oxysporum = ortholog

FVEG_02379

MASNTFMAVQETIAALTWQQTGCLLAGIWLIYVVQLVIWRLWLSPLAHIPGPKLAALTQYY

EFYYDFVLGGQYTYKIIDMHEQYGPIVRINPWEVHVGDPEFFSDLYTGPSRRRDKWTFYTQQ

SGAPRTRVPHPTALAGPKTATEGSLAAIDHSLHKLRRSALSPFHSTQTVRELQPVIEERVEALLGAF

LNYAEVSNGQPLDVMYPYSAFTNDVINEYAFARSDHLVE

KPDFGAEVTNDLLIGTHMGPCVQQLDWVLTLVNALPESISNRCMPGWGGFLKMKNDILEQI

HSVDPPQSSGKWMMDGGHPTMLSTKTRSVRDKTASRQTQ

ESQITVQGGTLTTSWAMSLATFHLLNRPETLRKLRDELFDAIPDAYETTPLSKLEKLPYLR

GVVKEALRLAIGTSSRLARVAPDEALVYHDRENDEEWNL

PPGSIVGMSPYKTVMDEKIFYDARGFHPERWVEDGERLDKYLDIFCSGSRICLGMALAHAE

LYLTLAKLFRRWGSGGVVYGSDDGDQRFGDVGYLSIFET

RVRDCEIDADYFMPIPYKGSEGFRFVFETY*

 

>CYP543A1 N. crassa AABX01000408.1

27399 MPCAFAITLLTVLVVLLVVQRWLERKRLRGGSRPLPGPT (1) 27515

27652 NLPFIGRVHDIPEKGSWLKFHEWSKVYGPIYQTKMFGVTHVWIS

      SEKIAHDLLSRRANIYSDRPQIPNLPDNRTSGDYLALLGRN (1) 27906

27974 DTWRRQRKLCNHLMHTSALASLHDYPTLERNRFLYLLSQSP

      ENYIEYIEQFTSRTIARLSWGSAHPAQILRHTTFGLLETIS

      PSGALPNVISFLRHLPLALSPWQKKEKARHDLEDKQFRSNI

      GFVKRMMDVGRAEPSFIRTYLEEQQGSINSTSTNKKCDEQK

      GKEAAADEAMHVVGLMAIAGALTIGSPIQSYILAMCHYPEW

      QARLQEEIDTELGGRCPMWEDREKLPLLRAVVKEVIRWRPPVPT (1) 28720

28825 GIPHAVEKDDVYNGYFIPAGATIHALEW (2) 28908

29018 AITRDEQTYPQAETFNPSRFLVPSYPTYREPLTIYPNLSGF

      SQFGFGRRTCQGVPIVEQDLFLTMGGMAWAFTISKKRDPVTGVEMPVHWNDYT

      PLLIAKPCRFPFDAVPRDEEKRKKMREMYEE

 

>CYP543A2 Magnaporthe grisea AACU02000514.1 revised

MASVPFVLFIAAWCLLIVKLYLDHQKRHRLPDGAKPLPGPKSIPFIGRVH

DLPPDMTWMKFYEWSKIYGPIYQMEIFGSVHIWMSSEEIVQDLLARRANI

NSDRPVIVNLPDNRTSGDYLALSGNNETWKRQRKMCNQLMAASNKQGLHA

YPTRERDRFLSLMANDPSNYIEWIEQFTARTVSRVCWGTPHRQALLRKTT

FGLLESISPAGALPNVVAWLGYLPDKFSPWRKKEHLRHALEAELFTSNLA

GVKASMNEGHSVPSAARTFLEDMEQKPRGSQAEADFHSAEGSKVVGMMAI

AGALTIGSPIQSFLLAMCHYPEWLERMQAEIDINLDGRCPQWDDREKLPL

LRAVVKETMRWRPPVPTGIPHASDKDDVYNGYFIPKGATLHALEWGLTRD

ESVYPDAETFNPDRWLNPSFPTYKEPLTHYPNLNGYSQFGYGRRTCQGIP

IVDQDLFLSMGGLAWAFDIRKKLDTQGNEIPVHWNSYSSLLIAKPVKFEF

DCVPREGKGRVIDLMASSAREMEEE

 

>CYP543B1 Mgr023 Mycosphaerella graminicola

40% to 543A1, 38% to 543A2 next best is 35% to CYP5445A1

yellow from JGI model estExt_fgenesh1_pg.C_chr_40312|Mycgr3

MSQVVQAAQEPSRYAFEQSIDNATVLEPVSVDTPIPADFSILNSKAVLGFSLLVIILVTRLFGQKKKLPAGAKPLPKLPG

IPWIGRFWGIPISGAEAAWH

FGDFHKSYGPIYEWLTFGVTHIWIENDKIARDLLVHRGKLYGDRHELPAAVGVKGGSEILPLMGIGENFWRHKNFIHTIM

RHSSQAGFWDAPVTENKHTLRRLLDSPDTWSESIITHCARVVATIAWGDPKHGTKLLTVVPQLLKAVSPEGPLPNLLPFL

MHLPAAISPFKKAEAERKRIMQEAFYEAQQDVMARMKAGTAGQSWSRIWLENEKGMEKSKLDQHEAAHAVGTNSFVAIAT

IGSPLHSFFTAICHYPSWLPRIQEEVDRVCGDRLPTMKDIPNLPVVRAVCKETLRWRQPTPLGVPHITTEDDVYDGYFIP

KGAMVHANHYLISREEATYPEGNEWRPERWLEPSWPTYKEPLTEYPTLRGDPGFGYGTRSCPGTDLVMTELYTLIASLVW

AFDIKRKEGRAGYDNPVPWYETNPFVITMAKPFPCNITVRSEEKRR

 

>CYP543C1 Mycosphaerella fijiensis

44% to CYP543A1   N. crassa

e_gw1.1.178.1

MALVTLALAVTAILVVKYIEASRQERRSATRKLPGPRGLPLIGNIHQLPNKCSWLKFHEWAVKYGPIYQVNLAGTNHVWI

SRDKEAHDLLSKRSAIYSDRPFIPALEHDNRTSGQYLPLMSKNALWSRQRRFAKQIMERSQKDGFYSYPELEAIRLLFEL

INDPSAYNTSMESFVARVTSRLAWGTSVGADELKQRARELLIGVSPTGALGNKLPAVMSLPESLSPAKAWEHRRGRTERK

FFQTMQDNVRHSLDATEQLRMSWTRMFLENKRLWGFSDDLEGAFAVGMHGIAGALTIAAPMQSFCLALCHFPQHQAMLHE

EVDRVLGDRLPTTADMPKMPVLRAFIRETLRWRPPVPTGIPHALTDDDIYKGYHIPKGSVIHPLEWSISRDPEVFPDADT

WNPLRWLDSEYPTYQEPLTQFPTITQYSQFGYGRRICQGMGVAEADLFVGLGSVAWLFSISKEQEQEPGPAYVDNDPTLN

FSTLLIAKPLPFKFDLRVRNQARAEQVTRAFLEQKEKGEFEEERVFWKNNNQGDAEVG*

 

>CYP544A1 N. crassa AABX01000494.1 cont3.721 NCU10011.1 (version3)

(GC BOUNDARY at exon 2)

13303 MGIIHEKAHYVVAVALVAFLIRAFVLSQWNTIKRNGERLK (2) 13184

13120 KPPNTLPLVGNGLQFLQSRWKLFSWFDACQRKFGYETVAISVPT

      LPPGVLIHDPRNLDYVFKNEGIFTKGNFVKGRTWDLFGNGIINA

      EGDFWKTQRKAGLSFLNTANLRVLTDVALPQYLSESISQLRSST

      NGTVVDLQHVFHEITTKLMGKMAYN (0) 12650

12590 MAMHADDEFTVSFDYASGG

TAERFQNPLWFVTEIFLGAELRKSIAVVKNFGRHIVTKAVQDRQEKEFGE

EEGKLDQISGSLIQSLLDAIQDEQMVADAALTYLSAGRDTTGQALTWTFY

LLMRHPRVVAKIREEATQLLKEKNVTLTPDQFDSSLFNPVTMPYSMAVFY

EVLRLYPPIPFEIRQCNEDVTLPDGTFLPKSSILVWCLWAMQRSKLTWGD

DADEFRPERFLDGNKLISRSPSEFPVFYGGPRTCLGRKMAEAIAAQVIPT

MACLFDFVPTSDEERTSKTSLTLPMEGGLPVTVKTLTGEEREKLVRIPEI

RFSEH* 11616

 

>CYP544A2 Magnaporthe grisea MG00452.4  58% to CYP544A1 AACU01001420 cont2.89

MTLLEFSPWAPAILVPVALIVLYTFLAAPRLKRNGIPLKTPRDSLPFVGN

GIVFLQPRQKLFAWFHQREREFGLETYQITVPTLPPGVVISDPVCLEYVF

KNETLFNKGAFFKERSDDLFGHGIINVDGEPWRAQRKAGLNFLNTTNLRV

LTDVALPRYLNENIGWLKNKADGETVADLQEVFHRITSQLMGKMAYNMEM

HAGDDFSVAFDYASGATAERFQNPLWFITEFFTGSRLRRSLAIVKEHGRK

IVANATCDRKAAEKLSATLPGVDADSEHDGLEEISGSLIQSLLDCIGDEK

LVADAALNYLSA (1?)

GRDTVAQ

ALTWTSYLLMQHPEAIERIRAETAELISPMDGSAGNHTTLLKPPSAPYTM

AVFYETMRFYPPIPFEIKQCVRETTLPDGTFLPASSVVVWCPWAMNRSKA

IWGSDVDEFRPERWLSLDGKSFASRSAAEYPVFNGGARMCLGKKMADIIA

VQVLPSIVTNFDFRPAYEKQQERVSKSSLTLPMQDGLPVFVKRRDVAR*

 

>CYP544A3 Fusarium graminearum FG08377.1 AACM01000335 FGcontig1.335_scaffold5

MALLSVNLRDGLILTAVITILLYIWEKKTAKITRNGEPLRKPPNTLPLVG

NGIIFLQPRQKLFSWFHRCERLYGYETLHITVPSLPPGVIVNDPQNLDFI

FRNEGVFEKGEFFKQRSWDLFGHGIINVDGEFWRLQRKAGLRFLSAAALK

TLTNEKLPKYLDQAIGVLEAKTNTKEVVDLQAVVHEVTTQLMGRMAYNME

MHADDDFTVAFEHASGGTAERFQNPLWFVTEMFTGARMRRSITTVKAYGK

RIVTSAVADRKEAEGKTQSDAPGSLIQSLLDSIGDETLVADAALNYLSAG

RDTVAQALTWTLYLLMKNPHVATKLRQSVESLRDEDTSTGDDPELLTPVR

LPYVLAVFYESLRLRPPIPFEIKQAQQATTLPDGTFLPAGAIVVWCAWAM

GRSHTTWGPNADDFRPERWLTTSLAGDVTVAQRPAAEFPVFNGGPRTLAR

LVPLFKFEPAFEGERVSKSSLTLPMEGGLPVYVQHQTPS*

 

>CYP544A4 Nectria haematococca

fgenesh1_pg.scaffold_1000764

Necha1/scaffold_1:2520778-2522398

77% to 544A3 note 544A3 missing part of heme signature

This gene model seems correct

MTLFNITMERILLLIAVIAIILVAREKRTPKITRNGESLR (2)

KPPNTLPIVGNGILFLQPRQRLFSWFAQCE

RLFGYETLHISVPSLPPGVIINDPRNLDFIFKNEGIFEKGDFFKQRSWDLFGHGIINVDGELWRLQRKAG

LRFLAAPALRALTSVRLPEYLEQAVRALKEKAETKEAVDLQAVVHEITTQLMGRMAYNMEMHADDDFTVA

FEDASGATAERFQNPLWFLTELVTGGRMREAIAIVKAYGKRIVTSAVADRARAGDEKAQSDSPGSLIQSL

LDSIGDEGLVADAALNYLSAGRDTVAQALTWTLYLLTRHPHVTTTLRQSVQSLHDEVGVVSEKPLDPELL

TPTRLPYVLAVFYESLRLRPPIPFEIKQAQQATTLPDGTSLPAGAIVVWCAWAMGRSQTTWGPDADDFRP

ERWLTTSPSGDMTVTQRPAAEFPVFNGGPRVCLGKKMAELVAVQTLARLVPMFDFVPAFEGERVSKSSLT

LPMEGGLPVHVQYKSTSTVER*

 

>CYP544A5 Nectria haematococca

e_gw1.40.11.1

Necha1/scaffold_40:94653-96155

50% to 544A3 same gene as fgenesh1_pg.scaffold_40000034

model is short at N-term added new exon 1

MLALYMLLLVLVLPLWVFMTERFKPSRIKRHGVPIR (2)

RSPGALPLLGNGLVFLRPRQKLFGWFTE (2)

CIQKYGQETLGIVIPILPPGVIIANPVNLDFIFK

HEELFKKGQFFRSRLQDLFGYGIVNVDGDLWRRQRAAGTHFFNGATMRTLKEIELPRALQQAMSQLDQHA

NIGTAVDLEAVLHELTTQLIGRLAYGVEMHAHDKFTKAFDHASFEIAKRFQNPLWRLTELITGGKLTESV

RVIKRYGQELVAQAVARRNGKYDERDLEERNAAPSLIDALFDSLGRQELVADSALNYLSAGKDTTAQALT

WTFYLLILHQGVAEKISRLVNSTESWSTQDSSCQSPRIQQNLSSETTHFILAVFYESLRLYPPIPFDMKQ

AQNYTTLPDGTFIPKDSVVLWCTWAMNRSVKTWGEDAHIFRPDRWLEGGRIKQSSTGDFPVFQGGARLCL

GKKMAELIAVQVIVALTRSFTFEQAFEGAKTSPTHLTLPMEDGLQVFIKHQCFERQREGTTV*

 

>CYP544A6 Fusarium oxysporum

75% to CYP544A4 Nectria haematococca

frameshift = &

FOXG_03445

MDYLSFVLEQPIVLSTTLLLFLYLLGKKSPKPTRNGKPLR (2)

RKPPNS

LPLVGNGIMFLQPRQRLFSWFHRCERLYGYETLHITVPTLPPGVIINNPQNLEFIFRHEG

IFEKGEFFKQRSWDLFGHGIINVDGEFWRLQRKAGLRFLSTAALKTLTSDRLPEYLEHAI

HVLKGKEAERDVVDLQAVIHEVTTQLMGRMAYNMEMHADDDFTVAFEHASGATAERFQNP

LWFVTEMFFGTRMRRSIRTVKAYGQRIVKSAVADREETEGKTQSDAPGSLIQSLLDAIGD

GDLVADAALNYLSAGRDTVAQALTWTLYLLMKHPEVTNKLCQSIQDLRDEVRDQDHSEND

PELLTPVRLPYVLAVFYETLRLRPPIPFEIKQAQQETILPDGTFLPKGAVVLWCAWAM &

GRSHTTWGPDADEFRPERWLTTSPSGDVTVMQRSAAEFPVFNGGPR

VCLGKKMAQLVAVQ

TLARLVPLFDFKPAFEGERVSKSSLTLPMEGGLPVYVHARKTSETS*

 

>CYP544A6 Fusarium verticillioides

94% to CYP544A6 Fusarium oxysporum = ortholog

FVEG_02317

MHYLSFVLGQPIVLSVTLLLFLYLLGKKSPKPTRNGKPLRKPPNSLPLVGNGIIFL

QPRQRLFSWFHRCERLYGYETLHITVPTLPPGVIINNPQNLEFI

FRHEGIFEKGDFFKQRSWDLFGHGIINVDGEFWRLQRKAGLRFLSTAALK

TLTSDRLPEYLEQAIHVLEGKEAGRDVVDLQAVVHEVTTQLMGRMAYNME

MHADDDFTVAFEHASGATAERFQNPLWFATEMFFGTRMRRSIRTVKAYGH

RIVQSAVADRKGMEGKTQSDAPGSLIQSLLDAIGDDDLVADAALNYLSAG

RDTVAQALTWTLYLLMKHPEVTNKLCQSIQDLRDEVSDRDRPEDDPELLT

PARLPYVLAVFYETLRLRPPIPFEIKQAQQETILPDGTFLPKGAVVLWCA

WAMGRSHTTWGPDADEFRPERWLTTSPSGDVTVMQRSAAEFPVFNGGPRVCLG

KKMAELVAVQTLARLLPLFDFRPAFEGERVSKSSLTLPMEGGLPVYVHARTTSET*

 

>CYP545A1 N. crassa AABX01000329.1 cont3.414 NCU07092.1 (version3)

9756 MQLLTLVIVGLFMLIVAVVHFIKAFREVNDPNGI

PGPTQIPYLGRVHDLPIQFMWLKFKEWADKYGQQGFYRTMMLGAEFIVVT

DEKVAEDLLVKRAKYNSDRPVIQSLFDSKSTHGSMEYLPLM (1) 9382

9297 AYWARQRKLS

HSYLTEATKAHYYGVMYFEVQRWMARLLENPEDFQHSIEDMSSKVMCQLTWDDP

SLSEYCTKSAWGLLTQMSPAGPITNVLTPLWHLPTLINPWKRAERKRHDE

QQAWWMERLLTCREKLARGELRPCWTRQFLEKTSQKTSISGDYEASCVIG

MLALVGIFTVVGPMSYWLVSMVHNPKWQEAVQREVDEVCGNRMPRLEDAP

RLPILRACIKETMRWKPNVPTGVAHETEADDHYQGYFIPKGTRILPFDW (2) 8509

8448 SFLRNPVKYPDPENFRPERWLEPGWPTYKEPLTQYPTIKGLTSFGWGQRQC

LGMSLTQDELIVGCGALAWLFNLRHKRDPITGRELPVPLDRSNSLLIIKP

DPFQMEFEPRSKERKKEALRVWKESEAKDRARRERWLRNVKEGKPNVIKE

PKVLQPTVKIPSPSPAAAVPAALVDGGEVRDELSKTVQVVKEKSGGVNGH

GDSLAEKAVMDVKKKADISITIARLDSTACVY* 7747

 

>CYP545A2 Mycosphaerella fijiensis

62% to CYP545A1 N. crassa

e_gw1.1.252.1

MQTLTIVAALVAFIGFAFFSLLNAARSSHPWKTKASEPPGPALVPWIGRIHDLPIQLMWLKFKEWADQYGPIYRTKMLGA

TFIIISDEKIAEDLLVKRAKVFSDRPEMKSLFDSKSTTGTMEYLPLMGKNEYWARQRKFTHSYLTEATNARYYGIMNHET

KRWLKRLLDDPDNFGFQLEDMASKVMCQLTWDDDSLSEAMTPSAWGLLTQMSPAGPITNVITPLWDYLPETINPWKRTER

KRHDEQQRWWMDNLVNVRTKLAKGLARPSFTRTYLEGLQNGKTGGLSGDYEASSAIGMMALVGVFTVAGPLYYFLLAMVY

HPEWQRKCQREIDEVCKGQMPDISDMPNLPVLRACIKETMRWRPNVPTGVAHEAEADDFYEGWLIPKGARILPLDFAFMR

NPEKYPDPEAYRPERWLEAGWPTFKEPLTQYPTIMGMSSFGYGQRQCLGQSVTRDETLVACGGLLWGYNLKKKLDSNGIE

INPPLDKSNSLLIVKPDPFEMAFEPRSDSRRDEILQNWKKTDLEDRTARAAFISKATESEKASAVPCRVDSMDPANDYFK

VQHATVAATA*

 

>CYP546A1 N. crassa TWO PIECES AABX01000340.1 cont3.411

16866 MLSDKLLDLFALHKTQEALADVNLYKLLVLALALVLTAIIIDYG

      YMLWLRSKMPTGPFPWPIIGNTFSLPENKPWIYFEELSKKYEKP

      LITFWIGR 17153 (2)

17210 NPTIWINDAWCAHEILEKKAQIYASRPRMVVFGELGTGQKNLVT

      MRILNNNDRDRWRIHRKLMHLGVGIQSVRGYREIQNNESKVVAL

      DYLREPKEYVKHLERYATSVVSVIAFGRRVASYDDPIITEVIAL

      MQLAADLNVPGKRFPMLLETFPXXXXXXXXXXXX 17671

145 RGGGQKGGGGGHYFFHSLALEALAQYESKSPLAKASMPTPYIKTLM

 283 DASRQYSLPEAELSSLTGNLFGAGSDTSSSTLITFMLSCLAFPDA

     MHKAQAELDRVLGGPAGGRSPHWDDAPNLPYINAYVKEVLRWRSV

     AIIGGQPHSNTSPDYYKGYFIPPHSWVQGNVWAIHHHEREFPDPD

     RFYPDRYLPGNDHHRPFPGEKGYMTFGWGRRVCSGQALAEQGTWI

     SVARLLWGFTIRKYRDPQTGKEEEVDIFAYTNGLNMRPQPFRCEI

     VPRSEEIREIIVREGEQALRDLKVLDGENRYRMSTFYQQKKREVA

     EMPEFDEKGNIRMVKVK* 1146

 

>CYP546A2 Magnaporthe grisea MG06557.4  64% to 546A1 AACU01000188 cont2.1218

MIFDRLTSQASLAKLEDVISNVPSSKLITLSVLIIAVTALIIDYGYMLYL

RSRMPPGPFPLPIIGNTFSLPDNKPWVWFDELSKRYNAPLVTIWIGRNPT

VWICDAWCAHDMLEKKAQLYSSRPRMVVFGELGTGQANLVTMRTRNQAER

DHWRVHRRLMHLGVGTQVVRRYRGVQDAESRVVALDMLRDPAAFVSHLER

YATSVVSILAFGRRVASSSDPIITEVIAVMQLAAELNVPGKKFPMLMETF

PILAKFPRSMPWMKGLGARGRKGGHYFWHSLAQEAVEQLDSRTDEERRNI

PKPYVHTLFAENDKYKISTEEISNLTGNLFGAGSDTSASTLVTFVLACCA

FPEPAHKAQRELDRVVGHGRSPSFGDQADLPYVNAFVKELLRWRPVAIIG

GQPHSPTADDDYKGWRIPANSWVQGNLWAIHRNPRDFPDPDRFEPDRFVE

GSEWQRPFPGDRGYMPFGWGRRVCSGQALAEQGVWITVARLLWAFDMRKK

RDPVTGAEVPVDIFAFTNGLNMRPQPFLCDITPRSEEIRRTVESEGLDAL

VELKPLDGESRYRFSTYYQQKKKESG* 74634

 

>CYP547A1 N. crassa AABX01000481.1 cont3.599 NCU09419.1 (version3)

16175 MPPTGNPFNFARITLLALVEVLIVKKTSYRHVLAISTTSLVLAILAINYV

FFLAWKLYLYPVYFHPLSKFPAPKVVDLWRVLARFRGKVPPGQLLLELAE

RTPNDGIIILQGGFGTSMLITKPAPLADILVHHPYDFVKHDAIRNFLRPI

LGDGLVIVEGEQHRFLRKNTQPAFKFGHIKELYSTMWTKAIEMNHVLKEE

LREKGNDTSVEINAWAGKVTLDIIGIAAFGRDFHVLERPDHPLVKNYADL

LEPGPAKFAYFFLALTLSRKFVDLFPWEISRRFNRTTSNIRRICAELVRE

RKAAIEKLGDDQFDILSLLIRSNNFSEAELADQLLTFLTAG (2) 17197

17248 HETTSSAFTWAVYKLAQDGEMQSTLRSELKRALPDFPRFAPGQD

IAIILEHLPYLNGVMNETLRLYPTVPMTVRTATCDTTVLGHPIKKGTEIM

ISPWLINRYSDNWPSASTEFVPERWIDQEGKHIAEDGTEQPTQKPNNTGG

VTSNYAQMTFLHGPRSCIGQGFAKAELRCLLATFVLAFEWKLGMDEKDVM

PDGVITIKPNNGMHLILRSLDGHQKG* 17910

 

>CYP547B1 Fusarium graminearum FG11303.1 AACM01000464 FGcontig1.464_scaffold9

MAVMSKLLNFPSLTLAACIEGFFAIKLFPNYYSTQSHLAAVVTILLINYA

FGVVFWAVLYPRLFSPLRRIPGPKAYLSAAHHSLVVKGRPSGDLFLDLAK

EYPGKDVIMLNSFRNQLCIMNPQLLADLLVHNCYDFAKPKRISGFLRHVL

GDGLIIVEGEQHKFLRKNSTPAFHFRHIKELYPMMWTKSETLTKAIAQDI

TASRSPVVELNGWASKVTLDIIGIAGLGRKFDAVEKKIDPLADIYEQLLE

PDREKLIFAMLSLAIGLPIIRMIPWKMNDLFNYLTGSLNDLCYPMIKEKK

AAIIEKGDDHFDVLSLLIKSNNFSDEALKDQLLTFLAAGHETTASALTWA

CYLLTQYPDIQSKLRDEVRDSLPADVDCNTPDLASILEQMPYLNGVMHET

LRLYPTVPLTMRSALRDTRIGDQYIPEGTDVIVSIWYINRAPEIWGPDAA

EFRPERWMTEDGKPNQNGGASSNYNFLTFLHGPRSCIGQGFAKAEMRCLL

ANMVKSFEWTLAMDNKLVLPRGVITIKPENGMYLNMKAI*

 

>CYP547B2 Nectria haematococca

e_gw1.60.8.1

Necha1/scaffold_60:53804-55237

77% to 547B1, add N-term exon and extend exon 2

MTVVSKLLTFPNLTLAAIVESLLVIKLFPSYYSSQSHVAAVATILGVNYAFGILFWALLYPVLFSPLRRIPGPR (0)

AYLSAAHRALVVKGRPSGDLFLDIVEQYPGQDLISLNALRNQIFVTNPRLLADVLVHRCYDFAKPTRISSFLRHILGDGL

IIVEGDQHKFLRKNTTPAFHFRHIKNLYPMMWNKAEILTRTLNQEISTTRSPVIELSPWASKVTLDIIGI

AGLGRKFDAVEKKQDPLAGIYEELLEPDREKLIFAMMALALGLPFVRLIPWKMNDLFNHLTGSLNNICRP

MIQEKRVAITEKEDDHFDVLSLLIKTNNFSDEALKDQLLTVLAAG (2)

HETTASALTWACYLLTKHPEIQQKL

RDEINEALPQDVESDSTIDLAGILEPLPYLNGIMHETLRLYPTVPLTMRQALCDTRIGDQFIPEGTDIII

SIWYINRSPEIWGPDAGQFRPERWITEEGKPNQNGGSSSNYDFETFLHGPRSCIGQGFAKAEMRCLLAAM

VRAFSWTLAMDDRLVLPRGVITIKPESGMYLKLTPLAPSI*

 

>CYP547B3 Fusarium oxysporum

80% to 547B1 Fusarium graminearum

FOXG_13409

MAVVSKLLGFPSLTLAAVIESFVAIKVFPDYYDSKSHLAAVFTILLVNYAFGIVFWGFLYPVFFSPLRHIPGPREYLSAA

HRSIAVKDRPSGDLFVDIANRYPGEDLLTLNSFRTHILVTNPQLLADLLVHNCYDFTKPKRISSFLRHILGDGLIIVEGE

PHKFLRKNSTPVFHFRHIKELYPMMWEKSQSLARAIQQDMTTSRSSVVELNSWASKVTLDIIGIAGLGRRFDAVEKKKDP

LADIYEGLLEPSREKLIFSGLALAIGLPIVRLIPWKMNDVFNYLTGTLNELCYPMIQEKKAAIIEKGDDHFDVLSLLIKS

NNFSDESLKDQLLTFLAAGHETTSSAITWACYLLTKHPEYQAKLREEVRNGLPEDLAANPTVDLAGILEQLPYLNGIMHE

TLRLYPNVPLTMRSAIRDTRIGDQFIPEDTDIMVSIWYINRSEAIWGPDATEFKPERWITDDGKPNQNGGASSNYNFLTF

LHGPRSCIGQGFAKAELRCLLATMVRSFEWTLAMDDKLVMPRGVITIKPENGMYLDLKAL*

 

>CYP547B3 Fusarium verticillioides

95% to CYP547B3 Fusarium oxysporum = ortholog

FVEG_11844

MAVVSKLLGFPNLTLAAVIESFIAIKVFPDYYDSKSHLAAVFTILLVN

YAFGIVFWGFLYPVFFSPLRHIPGPRDYLSAAHRSIAVKDRPSGDLFVDIAN

RYPGEDLLTLNSFRTHIMVTNPLLLADLLVHNCYDFTKPKRISAFLRH

ILGDGLIIVEGEPHKFLRKNSTPVFHFRHIKELYPMMWEKSQSLARAIQQDM

TTSRSSVVELNSWASKVTLDIIGIAGLGRRFDAVEKKKDPLADIYEGLL

EPSREKLIFSGLALAVGLPFVRLIPWKMNQVFNYLTGTLNELCYPMIQEKK

TAILEKGDDHFDVLSLLIKSGNFSDEALKDQLLTFLAAG

HETTSSAITWACYLLTKHPQYQAKLREEVRNGLPEDLATNPTVDLAGILEQLPYLNGVIQETLR

LYPTVPLTMRQAIRDTRIGDQFIPEGTDIMVSIWYINRSEAIWGPDATDFKPERWITDDG

KPNQNGGASSNYNFLTFLHGPRSCIGQGFAKAELRCLLATMVRSFEWTLSMDDKLVMPRGVIT

IKPENGMYLDLKAL*

 

>CYP547C1 Aspergillus nidulans AN8895.1 38% to 547A1

43% to 547B1 547 clan, 73% to 547C3, revised 7/18/07

MIPMLAATLAEAFILQRSVFTDTPLRTVVFGSAAVNLLILAIYNVWIWPF

FLDPLRHLPRAPGRFNMLRFIFDNPRGRLPLLWMKTVPNDGLIYFRDLLN

RTQLLATNHQALLDVMSTNTYDFEKPWRAREFLARIIGFGLILSEGAAHK

RQRRALTPFFTIKNVRALYTLMWDKTNQLLVEMDKQISRCPMEGTSPDSG

WGKVEMSVWARYFTLSRLTLDVIGPAAMGRDFRSLQNAENKVADSFLAIL

EPTKEKIAFLAINFLLPQWFAKKLPWRLNQVIEEQVGYLRDLCKEIVHEK

RAAITATKVTASELEADILGSMMLSGDFTDDELVDQMLTFLAAGHETTAS

ALTWTCYLLTLHPEVQDRLRAEIRTAIPHSTHPVTHSDLESLPLLNGVCQ

EVLRLYPTVPSTIREAVRDTTVAGKHVPKGTKIMLCPYAINRAPEFWGAD

GDAFRPERWIDTDPVTGKTSVNNHGGAATNYAQITFLHGQRSCIGKDFAR

AELRCAVAGVVGRYEFAMQDPKQ

VIHISGAVTTKPVEGMHLRMRRVEGW

 

>CYP547C2 Aspergillus fumigatus

AAHF01000014

MADTLLAIASVSLAEGYFLQRSVLKEYSFHSVCLGAVGVNLALK

IFWDFIIYPFFITANRHLPTVKGTFVNGKVIFDNPRGRLPLQWMKTIPNEGLIHFRDV

FNRSHLLPTTHQALLDIMSTNTYDFEKPWRAREFLARIIGFGLILSEGAAHKKQRKAL

TPAFNIKNIRSLYSLMWEKTGLFLDELEKEIRQNPMEGTSPEDGVGKVEMSMWAR (2)

LPSRLTLDIIGPAAMGRDFRSLHNPENKVADSFLAILEPTKEKMAFLAINFILPQWFARRLPWR

LNNVIDTETGFLRDLCKDIVREKRNTIVSSNMTAKELEADILGTMMVGGDFTDDELVD

QMLTFLAAGHETTASALTWACYLLTLHPDVQERLRTEIREHIPSGNHPISWSDLESMP

LLNGVCQEVLRLYPTVPITIREAVRDTTIAGKHVPKGTRILICPYAINRSPEFWGDNG

EEFLPERWIDHDKNGQKVVNHNGGASTNYAQITFLHGQRSCIGKDFARAELRCAVAGV

VGRFKFEMQDPKQEIHIAGAVTTKPVEGMHLKMSRVDEW*

 

>CYP547C2 Neosartorya fischeri

97% to CYP547C2 Aspergillus fumigatus = ortholog

NFIA_096320

MADTLLAIASVSLAEGYFLQRSVLNEHSFHSVCLGAVGVNLALKIFWDFVIYPFFITANRHLPTVKGTFVNGKIIFDNPR

GRLPLQWMKTIPNEGLIHFRDVFNRSHLLPTNHQALLDIMSTNTYDFEKPWRAREFLARIIGFGLILSEGAAHKKQRKAL

TPAFNIKNIRSLYSLMWEKTGLFLDELEKEIRQNPMEGTSPEDGVGKVEMSVWASRLTLDIIGPAAMGRDFRSLHNPENK

VADSFLAILEPTKEKMAFLAINFILPQWFARRLPWRLNKVIDTETGFLRDLCKDIVREKRKTIVSSNMTAKELEADILGT

MMVGGDFTDDELVDQMLTFLAAGHETTASALTWACYLLTLHPDVQERLRTEIREHIPSGNHPITWSDLESMPLLNGVCQE

VLRLYPTVPITIREAVRDTTIAGKHVPKGTRILICPYAINRSPEFWGDNGEEFLPERWIDPDKNGQKVVNHNGGASTNYA

QITFLHGQRSCIGKDFARAELRCAVAGVVGRFRFEMQDPKQEIHIAGAVTTKPVEGMHLKMSRVDEW*

 

>CYP547C3  Aspergillus oryzae

          GenEMBL BAE66568.1

          73% to 547C1

MDLMLALITLSLVEGFLLQRTVFVDLTFRTVTLSSVGVNLVLLTIWNTLIYPYFVTPLRHLPTISGNLNN

ARIVFDDPRGRLPLEWMKTVPNEGLIHFRDIVNRSYLLATNHQALLDIMSTNTYDFEKPWRARDFLARII

GFGLILSEGSAHKKQRRALTPSFNVKNIRALYSLMWEKTGLLMDELEKQIKQNPMDGTNAQSGEGKIEMS

VWASRLTLDIIGPAAMGRDFRSLHNPENKVADSFLAILEPTKEKMAFLAVNFALPQWFARRIPWRLNKVI

NDETGFLRDLCRDIVHEKRASITATKATAKELEADILGTMMLGGDFTDDELIDQMLTFLAAGHETTASAF

TWACYLLTLYPDVQERLRTEIRERIPSGNHPITWSDLETLPLLNGVCQEVLRLYPTVPMTIREAVRDSVV

AGKHIPKGTRILLCPYAINRSPEFWGNDGEEFRPERWIDTDKN

GQLVTNNTGGASTNFAQITFLHGQRSC

IGKDFARAELRCAVAGVVGRFRFEMQNPKQKIHIAGAVTTKPVEGMHLRMSRVDDW

 

>CYP547C3  Aspergillus flavus

100% to CYP547C3  Aspergillus oryzae

AFL2G_11890 revised

MDLMLALITLSLVEGFLLQRTVFVDLTFRTVTLSSVGVNLVLLTIWNTLIYPYFVTPLRHLPTISGNLNNARIVFDDPRG

RLPLEWMKTVPNEGLIHFRDIVNRSYLLATNHQALLDIMSTNTYDFEKPWRARDFLARIIGFGLILSEGSAHKKQRRALT

PSFNVKNIRALYSLMWEKTGLLMDELEKQIKQNPMDGTNAQSGEGKIEMSVWASRLTLDIIGPAAMGRDFRSLHNPENKV

ADSFLAILEPTKEKMAFLAVNFALPQWFARRIPWRLNKVINDETGFLRDLCRDIVHEKRASITATKATAKELEADILGTM

MLGGDFTDDELIDQMLTFLAAG HETTASAFTWACYLLTLYPDVQERLRTEIRERIPSGNHPITWSDLETLPLLNGVCQEV

LRLYPTVPMTIREAVRDSVVAGKHIPKGTRILLCPYAINRSPEFWGNDGEEFRPERWIDTDKN

GQLVTNNTGGASTNFAQITFLHGQRSC

IGKDFARAELRCAVAGVVGRFRFEMQNPKQKIHIAGAVTTKPVEGMHLRMSRVDDW

 

>CYP547C4 Aspergillus niger

fgenesh1_pm.C_scaffold_14000128|Aspni1

75% to CYP547C3

MDSLVLLGASLVEGYLLQHSLLPDWAFRSVTLLALAGNLILRLVWGVVIYPYWVTPLRHLPTLPGNLNHARVLSDDPRGR

LPLRWMKTIPNNGLIHFRDIFNRSFLLATNHQALLDIMSTNTYDFEKPWRTREFLARIIGFGLILSEGSAHKKQRRALTP

SFNVRHIRALYGLMWEKTGLLMDELQKEMDANEGCVEMSVWASRLTLDIIGPAAMGRDFRSLHNPENKVADSFLAILEPT

REKIAFLAVNVLLPQWFARKLPWRLNKVIDTETGYLRDLCKDIVAEKRAAILETKATAAELEADILGTMMLGGDFSDDEL

VDQMLTFLAAGHETTASAFTWACYLLTLHPTYQPLLRDEIRSRIPTASSPITHADLESLPLLNGIVQEVLRLYPTVPLTL

RESIRDTTVANTAIPKGTRLLLCPYAINRSPEFWGEDADMFRPERWVDTDKEGNQIVNGHGGAGTNYAQITFLHGQRACI

GKDFAKAELRCALAGVLGRYEVEMKDPTQVIHIAGAVTTKPVEGMHLRMRRVEGW*

 

>CYP547C5 Aspergillus terreus

82% to CYP547C3

ATEG_10355.1

MDSLLAAILLSLLEGFFLQRTYLGEYTFRTVTLGAFGVNLLLLAIWNLLIYPYFITPLRHLPTVPGNLNNARIIFDDPRG

RTPLHWMKTIPNDGLIHFRNLLNTSFLLATNHKALLDIMSTNTYDFEKPPRAREFLARIIGYGLILSEGAAHKRQRKALL

PSFNFKNIRAMYALMWEKTGLLMDELEREIALHPMDGTRPEDREGKVEMSVWASRLTLDVIGPVAMGRDFRSLQNTENKV

ADSFLAILEPTKEKMAFLAVNFVLPQWLAQRLPWRLNRVIENETTFLRNLCKDIVQEKRATIASTKATAEQLEADILGTM

MLGGDFSDDELVDQMLTFLAAG (0)

HETTASAFTWACYLLTQHPDIQDRLRAEIRARIPSGNHPITWSDLETLPLLNGVCQEVLR

LYPTVPVTLREAIRDTTVAGTHIPKGTRIILCPYAINRSPEFWGADGEAFRPERWIDTDK

NGQPVVNHTGGASTNFAQITFLHGQRSCIGKDFARAELRCALAGVVGRFRFEMQDPKQEI

HIAGAVTTKPVEGMHLRMARVDEW*

 

>CYP547C6P Aspergillus clavatus

65% to CYP547C2

RSPMGSCWQVSLLPSLRAGIAGRFRFEMQDPKQQIHIADAVTAKPVEGMHLKMSRIEGW*

 

>CYP547C7 Aspergillus terreus

54% to CYP547C5 Aspergillus terreus

ATEG_03033.1

MSSAAEQLAFLIPVTVVETFAVTATRMVPEQKPTSVFLYALGLNLVLLAIWNIFVWPFLVNPLRHLPTVRGPFIGASIFL

RHPRGSVTIPWLRTIPNEGLIHFREGLNRSFLLVTNQRALMDVLHTNSYDFTKPRGGREFLARGLGYGLILSEGDAHRAQ

RKAVTPAFAIKNIRAMYDLMWSKTQIFLHQLDREIQLHPVPGMKSGASGYVELGSWASRLTLDIIGPAAVGRDFQSLENE

DDPVSQAYSAILKPSSDTLLLFALSVLFPQWLVKLVPVRANIELPRRISYLRRVFHDILREKRTQLTEKPSDVDGDILGT

MMRGGEFSDSELVDQMLTFLAAGHETTANALTWASYHLALNPHIQETLRAEIRATVPSADAAVTWQQLESMPYLNGVMHE

VLRLYPTVPMTGREAIRDTTIAGQKIPRGTIISICPQSVNRSPEFWGDTADDFRPERWIDTDPVTGRQTPNKHGGAGTNF

AQITFLHGPRACIGKDFAKAEFRCAAAGLFGRFKAELQEGCVVKFGGTLTAQPVRGMPLRLTRLEGW*

 

>CYP547D1 Mgr008 Mycosphaerella graminicola

46% to 547B1

MAPPITVIGLLVTAVEAFVVASLFADHLPEHPLLRVFFRLLALNVGGYALYRIVIYPRLLTPLRNLPRPKGGKLLIGHGM

VTFERPNGVTFLKWMKEIPNDGLIYFRGFFNADRLIVTNGKILAELLVAKSYEFTKPAPVRSFLRNILGDGLIIVEGDDH

RFLRKNMMPVFSYRHIKNIYPLFWEKSVQLMDCVGQEIAEKESSNKNDTAVVEVNHWATKVTVDIIGMAAMGRDFQTLKN

TQDPLVEVYEELLEPTFEKQLFFLCQLMLPQNLVKKLPWKLNQTTEKCVNTLNTVTGQLVQDKKQSIKTESDSHKDILSN

CLKTGSFSDPQLVDQLLTFLAAGHETTSSALTWTTYLLATNPEIQTALRKELRDAIAIPTSPLPEDFDLASVLESLPLLN

GVCNEALRLYPTVPLTVRTSEKTTTLGNQTIPAGAQIILPIYAMNRLPEFWGADADQFTPERWIDTDEKSGERRPNNSGG

APSNYVNLTFLHGPRSCIGQGFAKAELRCLVAALAGRFEMEMADPNEDVIPAGVITTKPKNGMNLKMKLVEGW

 

>CYP547D2 Mycosphaerella fijiensis

63% to CYP547D1 Mycosphaerella graminicola

estExt_Genewise1.C_60812

MTSLPPSAVLVLASAVESAVAVALFPQHLPEHPLLYIFVRLLGVQTVAYAIYSIFIWPFFLSPLRHLPGPKSRNPIIGHG

MTMFEKPAGASFLTWLKTIPNDGLIHFRAFLNADRVLVADPKALQEILVTKAYEFEKPEPLRNFLRFVLGDGLIIVEGDS

HKFLRKNLMPVFSFRHIKELYPIFWSKSTQMIDGIAQQVRESAGPGESKTSVVEINHWATKVTVDIIGLAAMGRDFQALK

NSDDPLIQVYEELLEPTMEKQVYFVSQILGPAKLIRKLPLKLNERSEVIVRTLTDICLKLVREKKELIKTEPADHKDILS

LCIRSNNFSDEQLVDQLLTFLAAGHETTSSALTWTCYLLAKHPEIQTALREEIHRTIPSPNQPPPSDTDLAQIFESAPLL

NGVCNESIRLYPTVPITVRINPKPTTLAGHVIPKNTQFLLSPWAINRNPAYWGADADEFTPYRWIDTDEKTGEKRPNNSG

GAPSNYAILTFLHGPRSCIGQGFAKAELRCLVAAIVGSFEMTLANPEEKVVPHGVVTTKPKNGMHLVLKPLGPW*

 

>CYP548A1 N. crassa AABX01000409.1 cont3.400 NCU06895.1 (version3)

71638 MVFGAAHLQLWLGALVVLGAVY (0) 71703

71769 TSCLIFYRLFLHPLAKYPGPFLAKLTDG

YMAYHAFKGDRHLEFWRMHEKYGKFVRFGPNSLSVNSNTALKDIYGFKAN

VRKAEFYDAFVHPAPNTHNARDRDLHARKRRVLAHAFSDSAIKEVERYIL

SNIRTFCDAIGDLGRPSFAPLADKKGWSSPKNMSDWCSWLAMDILGDLCF

GKAFHMLERPDNRYAVDLVSVAAHRHLICGTMPMLDKLSLDRIFLRKIAS

GRAQYMAYSRQQLAERTALGDETDRRDFFYYLLKARDPETGQGFTTPELW

GESNLLIIAGSDTTSTAMAATLFYLVRNPTALARVTAEIRSRFASVEGIH

QGPSLQSCTYLRACIDEAMRLSPSVGGLLPREVLPGGITIDGEHVPQGTI

IGVPHYTIHHNAAYYPDPWSYTPERWLSTRSPSEKTSGAAMQTEEQIQLA

QSAFCPFSIGPRGCIGKGLAYIEMSITLARVLFLYDLRRAVGVDDPAEGG

KKGAEYGRHRVGEMQLVDTFTSTKDGVMVEFRPREDLAA* 73322

 

>CYP548A2 Magnaporthe grisea MG03375.4  74% to CYP548A1 AACU01000699.1 cont2.663

       MENLCTWLGLLAGAVFL (0)

124757 TSCLVVYRLYFHPLAKYPGPFLARISDVYQLYHAWRGDRHLEFWRMHQKY 124608

GKVVRFGPNCISVNSATALKDVYGFRANVRKAEFYDAFVHPAPNTHNARDRD

LHARKRRVLSHAFSDNAIKQMERYILANERTFCAEIGRGASPDSKGWSTP

KNMSDWCNYLAMDILGDLAFGKAFHMLESEDNRYALDMVGAAAQRHLICG

TMPIIDKLSLDKVLFPKIAAGRAKYMKYSRAQLTERTKLGDETDRRDFFY

HLLRARDPETGQGFSTPELWGESNLLIIAGSDTTSTAMAATLFYLCRNPA

ALARVTEEVRTAFSSVEDIKQGPQLNGLTYLRACIDEAMRLSPSVGGILP

REVLAGGITVDGETLPEGTVVGTPHYAIHHNEDYYPDPFAYAPERWIAGS

VLKSGRTVGEADIQAAQSAFCPFSVGPRGCIGKGLAYVEMSTTLARTLFT

YDMRKAVGVEDPSEGGKPGAEWGRHRESEFQLVDTFTSLKEGPICEFRER

DLSA*

 

>CYP548A3 Fusarium graminearum FG06068.1 AACM01000241 FGcontig1.241_scaffold3

MTVDLAHLWVYLGLLVGGGLFYGFSIVIYRVFFHPLAKYPGPFMAKITDA

YQLYHAWKGDRQIEFWRMHEKYGPVVRFGPNLVCFNSNKALKDIYGFRSN

VRKAEFYNAFVHPTANTHNTSDKEVHARKRRVMSQAFSESAMKEMQRYIL

GNVRTFCEQIGMLEGSGEDNKGWTKPRKMSDWCNYLAMDILGDLCFGKAF

HMLESPTNRFALQLVEAATTRHLLCGTMPIVNKLNLDKILFPGLAAGRAR

YMAYSKGQLTERTKLGEETDRRDFFYYLLKARDPETGQGFSTPELWSESN

LLIIAGSDTTSTAMAATLFYLVRCPRALKRVTEEIRSKFNDVEEICQGAT

LASCTYLRACIDEAMRMNPSVGGILPREVLSGGMTIEGHVIPEGTVVGVP

HYTIHHNESYYPSPYEYVPERWLVGTLNPLTGQNTTEAEVALASSAYCPF

SVGPRGCIGKGLAYVEMTNTLARTMYMYDMRKAVGIVDPAEGNPKNEWGR

HRPGEMQLVDIFTSAKNGPMVEFRKADHMSS*

 

>CYP548A4 Nectria haematococca

fgenesh1_pg.scaffold_3000360 same as e_gw1.3.949.1

Necha1/scaffold_3:1057950-1059702

MQLDLGHLWIYLGLLVGGGVLY (0)

TFGLVVYRVFFHPLAKYPGPFLAKITDAYQLYHAWKGDRHLEFWRMHQ

KYGPVVRFGPNSLCFNSNKALKEIFGFRTNVRKAEFYNAFVHPTANTHNTRDKEVHARKRRVMSQAFSES

AMKEMQRYILANVRTFCEQIGALDGSAEESKGWTKPR  KMSDWCNYLATDILGDLCFGKAFHMLESETNRF

ALGLVEAATTRHLL (0)

CGTMPIVDKLKLDKVLFPGLAAKRARYMAYSKGQLTERTKLGEETDRRDFFYYLLKARDPETGQGFSTPELWSESNLL (2)

IIAGSDTTSTAMAATLFYLVRCPRALKRVTEEIRSKFNNVEEVCQGAT

LASCTYLRACIDEAMRMSPSVGGITPREV LPGGITVEGRPIPEGTVVGVPHYAIHHNESYFPSPYEYIPE

RWMAGAVNPLTNEKTTEDEVALAASAFCPFSIGPRGCIGKGLAYVEMTNTLARTIYLYDMRKAIGIVDPA

EGKPNNEWGRHRVTEMQLVDIFTSAKNGPMIEFRKAETASA*

 

>CYP548A5 Gibberella moniliformis

DR630735 DR651021.1 DR621844.1 cDNA

90% to CYP548A4

also Fusarium verticillioides FVEG_04704

MTFNLTHLLVYLGLLVGGLVLYGFSIVVYRVFFHPLAKYPGPFIAKITDGYQLYHAWKGD

RHLEFWRMHQKYGPVVRFGPNSLCFNSNKALKEIYGFRTNVRKAEFYNAFVHPTANTHNT

RDKEVHARKRRVMSQAFSESAMKEMQRYILGNVRTFCEQIGMLEGSGEETKGWTKPRKMS

DWCNYLAMDILGDLCFGKAFHMLESPTNRFALGLVEAATTRHLLCGTMPIVNKLNL

DKILFPGLAAGRARYMGYSKGQLAERTKLGEETDRRDFFYYLLKARDPETGQGFSTPELW

SESNLLIIAGSDTTSTAMAATLFYLVRCPRALERVTEEIRS KFNDVEEICQGATLASCTY

LRACIDEAMRLSPSVGGILPREVLSGGITVEGRAIPEGTIIGVPHYTIHHNESYYPSPYE

YVPERWLVGALNPLTGEKTTEDEVALAASAYCPFSIGPRGCIGKGLAYVEMTNTLARTMY LYDMRKAIGIVDPAEGNPKNEWGRHRPSEMQLVDTFTSAKNGPMIEFRKASHIQA*

 

>CYP548A5 Fusarium oxysporum

98% to CYP548A5 Gibberella moniliformis/ F. verticillioides

FOXG_07777

MTFNLTHLLVYLGLLVGGLALYGFGIVVYRVFFHPLAKYPGPFLAKITDGYQLYHAWKGDRQLEFWRMHQKYGPVVRFGP

NSLCFNSNKALKEIYGFRTNVRKAEFYNAFVHPTANTHNTRDKEVHARKRRVMSQAFSESAMKEMQRYILGNVRTFCEQI

GMLEGSGEETKGWTKPRKMSDWCNYLAMDILGDLCFGKAFHMLESPTNRFALELVEAATTRHLLCGTMPIVNKLNLDKIL

FPGLAAGRARYMGYSKGQLAERTKLGEETDRRDFFYYLLKARDPETGQGFSTPELWSESNLLIIAGSDTTSTAMAATLFY

LVRCPRALERVTEEIRSKFNDVEEICQGATLASCTYLRACIDEAMRLSPSVGGILPREVLSGGITVEGRAIPEGTVIGVP

HYTIHHNESYYPSPYEYVPERWLVGALNPLTGEKTTEDEVALAASAFCPFSIGPRGCIGKGLAYVEMTNTLARTMYLYDM

RKAIGIVDPAEGNPKNEWGRHRPGEMQLVDTFTSAKNGPMIEFRKAAHINA*

 

>CYP548A6 Botryotinia fuckeliana B05.10

AAID01001234.1 cont1.1234, whole genome shotgun

59% to 548A5, 59% to 548A4

41% to AAKD02000022.1 Aspergillus clavatus

       MVELPSTSFILSGTLRALVFATTITAVY (0)

18189  LTTITIYRLTFHPLAKYPGPFLAKITDWYSVYHAWKGDRHLEFYRCHEKY (1)

17991  GPVFRYGPDSLSINSNTALKTIYGHKSNVKKSQFYSVFPPTKDTFNTHSSIDKA  17830

17829  SHARKRRVLSHAFSDNAIKTMEKYILGNVRTFCNNLGQPKASAEKSTGGWSVAQNM  17662

17661  SDWCNYLTFDVMGDLCFGKAFEMLENASNRHVIDLIGNAAHTHLI (0)

17481  TGTYPIIKTIGLSKILFRKIYAQRMKYMAYSRAQAAERTKIGLDTDRKDFFYYLLNARDA  17302

17301  ETGQGFTTPELWGESNLLIIAGSDTTSTALSAAFFYLVHNPDKLEILTKEIMSTFSDVEE  17122

17121  IHSSPTLNSCAYLRAVVDESMRLSPPVGGILPREVLPGGIDIDGLHIPAGYVVGTPHY  16948

16947  TIHHNPAYYPSPFTFLPERWIPSSSPQVTKDSVALAQSAFCPFSIGPRGCIGKGVAYVEL  16768

16767  MTSLARVVFMYEMRIAEGYTVGEGSEEMEVSRRRKEEYQLKDSFTSMKDGPYVEFRARAK*  16585

 

>CYP548A7 Mgr009 Mycosphaerella graminicola

61% to CYP548A6   Botryotinia fuckeliana yellow does not agree with JGI model

estExt_fgenesh1_pm.C_chr_130091|Mycgr3

MSLQYLASKLSFNLLALLPVGLAIYTFGLAVYRIWFHPLAKYPGPLLAKITDLHSTYHALNGDRHLEFWRNHEQYGPVYRFGPNSVSFNSNTALKEIYGFKANVQKAEFYQAFWASKDAFSTHSSIDKAVHARKRRVLSQAFSDGAIKSMENHILAHIRQFCANLAGNAESSTHPRYSDSVASTKAFGPTVDITDQSNYLAFDIMGDLCFGKSFNMLTHPDNRFPIDLIASASHRHLICGTYLPIHTYHLDKLLFRKIAAGRARYMQYSKAQAGERMKMGMDVHRKDFFYHLLSAKDPETGRGFAPAELWGESNLLIIAGSDTTSTAIAATIFYLVHNAEKLARLTSEIRAAFDDVEEIRFGQKLSSLPYVRACIDEAMRLSPSVGGLLPREVLTGGIEIDGEQIPAGTVVGVPSYTVQHNEAYFPSPWEFRPERWVAGSEKGVTIDSVARAQSAFIPFSVGPRGCIGKGMAYAEMTTTIARMVWLYDLRLTPG

STLGKGDPRFAYGRQRAAEFQLKDSFTSIKEGPEVQFRARS

 

>CYP548A8 Podospora anserina

EST CU891099.1

82% to 548A1

MAFGAAHVQLWLGLLAVAGLLYTS

CLVIYRVFFHPLAKYPGPLLAKLT

DAYMLYYAWRGDRHLEFWRMHEKYGKFVRFGPNALSANSNTALKEIYGFRANVRKAEFYD

AFVHPAPNTHNARDRDLHARKRRVLSHAFSDGAIKEVERYILANIRTFCEAIGDYGRAIQ

DNKGWSAPKNMSDWCNWLAMDILGDLCFGKAFHMLDRPDNRYAVDLVGVAAQRHLLCGTM

PIVNKLSLDKILFHKIAAGRAKYMAYSRQQLTERTALGDETRRRDFFYHLLKARDPETG

 

>CYP548A9 Mycosphaerella fijiensis

72% to CYP548A7 Mycosphaerella graminicola

estExt_Genewise1Plus.C_20402

MSLASTLSATPLSLLTLLPVALAVYFVGIVVYRLYLHPLAKYPGPFLAKITDLHQTYHALKGDRHLEFWRCHEKYGPVVR

FGPNSLSFNSNTALKEIYGFKSNVRKADFYQAFWASKDAFSTHSSIDKSVHARKRRVLSQAFSDAAIKSMENHILAHVRQ

FCQNLGGLTTFGSVPNSESKGYGEPIEISDQANYLTFDIMGDLCFGKAFGMLERPGNRFAIDLIGNAAHRHLICGTYLPI

HEWHLDKFLFRKIAAGRARYMQYSKGQAAERMKMGLDVDRKDFFYHLLKAKDPETGNGFTTPELWGESNLLIIAGSDTTS

TALAATIFYLAHNSSKLQKANEEVRGAFSNVEDINIGPTLGSLQYIRACIDEAMRLSPSVGGLLPRQVLPGGVEIDGQLI

PEGTIVGVPHYSIQHNASYYPEPFVFQPERWIANPGCGVTTESVALAQSAFCPFSIGPRGCIGKGMAYHELTLTLARMLF

LYDMRLAPGSNLGEGRPDLEHGRRRASEFQLKDTFTSMKSGPMVQFRARQ*

 

>CYP548B1 Fusarium graminearum FG00037.1 AACM01000002 FGcontig1.2_scaffold1

MHRAPNVLTAVDKKAHGMRRRILSQGLSDSSTRAFGNTIKKHIERLCQKI

EGHSDPNTQWSESYDMARWFSYLTFDIMADVVFGQPYNLLGNSEYRYVVD

SIEGSNIRTGVLIQAPEAYTWRLDKRLFPASIRHRNTFVKFISSLVQERL

TTKPLERDDIISHLLTAKDSETGQGFTKNEVAAESSTLIVAGTDTSSTAL

AATLFYLTQYPNMYRRAVAEVRSSFAKSQDVKLGRALNECVFTRACIEES

MRLSPPAASALWRRVQVGGQTVDGHAIQAGCNIGVCIYAIHHNELYYPDP

FVFNPDRWLQNDKQAQSAFSPFSVGPRSCIGKGFAMAELMLAVATILVKF

DIRRAPGDQGCIGQGHLEGEDGRRMVDEYQLHDHV

TAFKQGPVLQFRRRD

 

>CYP548C1 Aspergillus nidulans AN7881.1 43% to 548B1 40% to 548A2 53 clan

MLPTYFGTSNFLAAFAVWMGVVVLAFAIFCVRRLYFHPYSKYPGPLLGKL

TNYYAVYHSWKGDQHIDMWRCHEKYGPYVRYGPNELSINTAAGLKEIYSH

GRNFKKSVKYNAMVHQAANTLTTIDKRKHGKKRRLISQAFSDAAFRSYEE

TIQQKIAQLCTALRRRDDDSNEIVPDGNWGPAKNMSHWCDWFTFDVMCSV

IFGVPWSSLTEKTYRNVPHLIEVSNVRVGCLIEAGGSKNMKIDKYLFPAA

IAARNQFVKFVNDIIRQGMAMSAKGSLKGAFALLRDATDPETQEPLSFKE

LCGESATLVVAGTDTTSTALAASIYYLCNHPKVYERAVQEVRSTFQSRAE

IGLGPKVNSCTYLRAVIEESMRLSPSAPGPLWRQADAGGATVDGQYIPQG

LEAGTCVYAIQHHPEIYPQPFKFVPERWLGPEAVPEQYRSDYSPFAGFTP

FSIGPRGCIGKPLAYIELTLTLCHILYAFDMRLPQGVNVNEDAEYQLALH

ITAAKEGPLVEFRPRTVV*

 

>CYP548C2 Aspergillus niger

gw1.16.377.1|Aspni1

54% to CYP548C1

AM270216.1

MIDSQVLLTFALKGLLLGLIVLSCTVAG

LCIYRLFFHPLAKYPGPWLAKTTKLYAGYHAWRGDLHLDMWRCHEKYGDFVRYGPNSLLVNTADGLHDIYSHGKNFKKAQ

RYAAMVHRAPNTLTVIDKKKHGKKRRVISQGFSDSALKNHEVVILEQIQHLCTQLKAGEGGKPVPADTWSTPKNMGRMTD

YLAFDVMSNIIFDCPWSTLRFPTFRFVPEVIERSNVRVGTLAQSPELSIFRLDKYLFPEAIKARDMFIGFVDVMLTQGIE

AASKTGKGVFALLAKAKDPETGLPLRNRELGGESATLIVAGTDTSSTAMAACFFYLSHNRAAYERAVNEVRSVFSSPEEI

RMGQQMSQCVFLRACIDESMRMSPSAAGSLWREAEEAGATVDGQYIPPGVDVGTCIYSIHHNPAYYPEPFCFQPERWIAD

ECRSVQGDVGRARSAFNPFSIGPRSCIGKSLAYVELHLVLARVLWEFDIRLPEGDLGKTGEGKVGAEYGRHRLDEYQLYD

HLTAAKVGPYLEFCPRK*

 

>CYP548C3 Uncinocarpus reesii

75% to CYP548C2 A. niger

UREG_04460.1,

MLPAGDFVRYGPNSLLVNTAKGLHDIYSHGKNFKKAQRYGAMVHRAPNTLTVIDKNKHGKKRRIISQGFSDAALKTHEPV

ILKQVQQLKTQLSLDDAGRQVPSGLWTLPKNMARLGDYFTFDVMCNIIFDIPWSTLRDPTYRFVPDVIEKSNVRVGTLAQ

APELTIFRLDKLLFPEAIRARDKFITFIDKVLGEGIKAAGTSGKGVFATLTNAKDPETQQPLRMRELGGESATLIVAGTD

TTSTALAACFFYLSHNRSAYDRAATEVRGVFRSPGDIKMGPSMHQCTFLRACIDESMRMSPSAASSLWREAEEQGATVDG

EYIPAGVDVGTCIYSIHHNPEYYPQPFSFRPERWINDEAHKVKGDVALARSAFNPFSIGPRSCIGKGLAYVELQLTLAHI

FWHFDFRLALGEQGLVGEGKEGAEFGRHRADEFQLEDHLTAAKHGPYIEFRSRA

 

>CYP548C4 Aspergillus clavatus

82% to CYP548C2 A. niger

ACLA_093770

MLSPQVVSSFALRGLLLGLFALCCTTVGFVVHRLFFHPLAKYPGPWLAKVSNLYAGYHAWKGDLHLDMWKCHEKYGDFVR

YGPNSLLVNTATGLHDIYGHGKNFRKAQRYAAMVHRAPNTLTVIDKKKHGKKRRVISQGFSDAALKNHEVVINEQIRQLL

AQLKTGEGGKAVSVGSWSSPKNMGRFTDYFAFDVMSNIIFGVPWSTLRTPTYRFVPEVIEKSNVRVGVLAQAPEVTFMRL

DKYLFPEAIKARDIFIRFVEEMLNQGIQVAATTGKGVFATLANATDPETGLPLRKRELGGESATLIVAGTDTSSTALAAC

FFYLSHNRSALERATAEVRSTFQSPEDIRMGVEMNQCVFLRACIEESMRMSPSAASSLWREAEEAGATVDGHYIPPGVDA

GTCIYSIHHNPAYYPQPFTFRPERWIASECHTVKGDVSLARSAFNPFSIGPRSCIGKSLAYVELHLALANVLWAFDLRLA

SGDLGKTGEGVENAEYGRHRVNEYQLYDHLTAAKHGPYIEFSPRV*

 

>CYP548D1 Aspergillus nidulans AN9007.1 46% to 548A3 53 clan

MLQLLLPRSWPAALLAAGTILSFVWIAYYRFIHPLSRVPGPFLASVTPLV

QLYHGLKGDRHLWIYELHQRYGDHVRLAPNFVSINNVEGLHKIYGHGNKF

RKADFYNGFLAIPGVYNTHNAIDKLVHGRKRRVLSQAFSDTALKGMEDVM

LLHVRQLCSILGRERPTSQSGDKDGATFNMANWFGYLTYDVMGELCFGKS

FDMLIDGAKRRMIHLVDRAAYRHYVCGLWMPLHRWHLDQIFIRRLTNDRW

NFIMESRQEANIRAKERTSLGQDAKKDFFYYLLNARDPETGKGLATQELW

GEANVLMIAGSDTTSTSLSAAIFYLVRNPHALEKLKNEVRSHFSDVEEIV

TGSKLNQLTYLKACIDEAMRLAPAVPGSIPREASDPVVTVDGLVLPEGTG

CGTPPYCIHRRPDYYREPLSYLPDRWIEGSTCKTADAAWTVTREEVDLAR

KAFCPFSIGPRGCIGKSMALMEMRVTLARLMYLFDFELADATGEDENGHF

KMVDHFVVSKTGPNVINIITRSL*

 

>CYP548D2  Aspergillus fumigatus Af293

          GenEMBL XP_747856.1 also EAL85818.1

          70% to 548D1 revised 3/10/2009

MLVQPLLENLSVWGAVQGILSLLGVLTLAYVTYNRFFHPLRSIPGPFLASITPWVQLYHGLKGDRHLWLH

RLHEKYGSHVRAAPNFVSINTDRGLHDIYGHGKRLRKANFYNAFPAIKGVYNTHNAIDKAMHGRKRRVLS

QAFSDQALKSMEDVMLLHVRQLCEILTGGLDGPKGEKSATAVFNLGDWFSYLTYDVMGELCFGKSFDMLI

SSGRRKLIELVDRAANRHYV (0)

CGLWMPLDTWHLDQILIHKLTNDRWNFIMNSRVEANERAKERT

QAGHDAKKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTTSTTMSATIFYLVRNPRAMELLRKE

IRENFSSVEEIVTGPKLNELVYLKACIDEAMRLAPAVPGAPPREVMEGGAMIDGVFLPEGTDCGTPTYSI

HRQPEYYREPEVYIPERWIEGATCQAGSESWTTSKESVELARRAFCPFSIGPRGCIGKSMAFMEMRLTIA

RLMFLFDLELADHKGEDEKGQLALVDHFTSAKYGPNVVVRKRV

 

>CYP548D2 Neosartorya fischeri

96% to CYP548D2 Aspergillus fumigatus = ortholog

NFIA_048430

MLVQPLLENLSVWGAVQGILSLFGVLTVAYVTYNRFFHPLRSIPGPFLASITPWVQLYHGLKGDRHLWLHRLHEKYGSHV

RAAPNFVSINTDRGLHDIYGHGKRLRKANFYNAFPAIKGVYNTHNAIDKAMHGRKRRVLSQAFSDQALKSMEDVMLLHVR

QLCGVLTGGLEGKGEKSATAVFNMGDWFSYLTYDVMGELCFGKSFDMLISTGRRKLIELVDRAANRHYV (0)

CGLWMPLDTWHLDQIFIHKLTNDRWNFIMNSRVEANERAKERTQAGHDAKKDFFYYLLNAKDPETGKGLTTPEL

WGEANVLMIAGSDTTSTTMSATIFYLVRNPRAMELLRKEIRENFSSVEEIVTGPKLNELVYLKACIDEAMRLAPAVPGAP

PREVMEGGAMVDGVFLPEGTDCGTPTYSIHRQPEYYREPEAYIPERWIEGAMCQAGSESWTTSKEAVELARRAFCPFSIG

PRGCIGKSMAFMEMRLTIARLMFLFDFELADHKGEDEKGQLALVDHFTSSKYGPNVIVRKRV*

 

>CYP548D3  Aspergillus oryzae

          GenEMBL BAE62732.1

          81% to 548D2, 45% to 548A2

MLVQEVWKTLSIEGVVVGVSALLGLLSFAYVIYNRFLHPLRKFNGPFLASITPWVQLYHGLKGDRHLWLH

NLHQQYGSHVRVAPNFVSINSAQGLHDIYGHGKKLKKANFYNGFTAIKGVYNTHNVIDKTVHGRKRRVLS

QAFSDQALKSMEDVMLLHVRQLCTALAEQQTEGNKAEAQKTTVQNMGDWFSYLTYDVMGELCFGKSFDML

VSSGRRKMIQLVDRAANRHYVCGLWMPLDTWHLDQIVIHRLTNDRWNFIMNSRVEANKRAQERTQAGHDS

KKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTTSTTLAATIFYLVRNPRAMKLLKKEVREAFT

SVEEIVTGGKLNELVYLKACIDEALRLAPAVPGAIPREVMEGGAVVDGAFLPAGTDCGTPTYSIHRQPQY

YREPEAYLPERWIEGATCVTADDKWESTKDAVETARRAFCPFSIGPRGCIGKGMAFMEMRLTIARLMFLF

DMELADRQGEDEKGHLALVDHFTSAKQGPNVIVRRRDITV

 

>CYP548D3  Aspergillus flavus

99% to CYP548D3  Aspergillus oryzae

AFL2G_08138

MLVQEVWKTLSIEGVVVGVSALLGLLSFAYVIYNRFLHPLRKFNGPFLASITPWVQLYHGLKGDRHLWLHNLHQQYGSHV

RVAPNFVSINSAQGLHDIYGHGKKLKKANFYNGFTAIKGVYNTHNVIDKTVHGRKRRVLSQAFSDQALKSMEDVMLLHVR

QLCTALAEQQTEGNKAEAQKTTVQNMGDWFSYLTYDVMGELCFGKSFDMLVSSGRRKMIQLVDRAANRHYVCGLWMPLDT

WHLDQIVIHRLTNDRWNFIMNSRVEANKRAQERTQAGHDSKKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTT

STTLAATIFYLVRNPRVMKLLKKEVREAFTSVEEIVTGGKLNELVYLKACIDEALRLAPAVPGAIPREVMEGGAVVDGAF

LPAGTDCGTPTYSIHRQPQYYREPEAYLPERWIEGATCVTADDKWESTKDAVETARRAFCPFSIGPRGCIGKGMAFMEMR

LTIARLMFLFDMELADRQGEDEKGHLALVDHFTSAKQGPNVIVRRRDI*

 

>CYP548D4 Uncinocarpus reesii

72% to CYP548D3    Aspergillus oryzae

UREG_01325.1

MIVQPLVAHLSLASVGVGFLAVTTLLCFGYVVYNRLFHPLRKYPGPFLATVTPWVQLYHGLKGDRHLWLYALHQKYGPHV

RVAPNFLSINSARGLHDIYGHGKKVKKGDFYNAFPAIKGVYNTHNVIDKHVHGRKRRVLSQAFSDQALKGMEDVMLVNIR

QFCAIMAGDEPSLDSGAAKTERGLVVRNMADWFGYLTYDVMGELCFGKSFGMLIERGKRQVIGLVDRAAYRHYVCGLWMP

LDRWHLDQVFIRSLTNDRWNFIQKSRVEANQRAKERTQAGHEAKKDFFYYLLNAKDPETGRGLSTPELWGESNVLMIAGS

DTTSTSLAATIFYLVRKPTAMAKLQKEVRDNFTDVEEIVTGPKLNELVYLRACIDEAMRLAPAVPGAMPREVLPGGIDVD

GLHLPGGVDIGTPCYTIHRNEQYYREPNSFVPERWIEGAMCETDNKIWTSSKDDVDAARKAFCAFSIGPRGCIGKGMALM

EMRLTLARMMFLFDVEFADRTGEDANGHLHMVDHFTSQKHGPNVIIKKRQSV

 

>CYP548D5 Coccidioides immitis

87% to 548D4

CIMG_01401.2

MLLQPLVAHLSLLSVGVGFTALTALLTFGYCVYNKFFHPLRKYPGPFLASVTPWVQLFHGLKGDRHLWLYKLHLKYGPHV

RVAPNFLSINSAQGLHDIYGHGKKVKKGDFYNAFPAIKGVYNTHNVIDKHIHGRKRRVLSQAFSDNALKGMEGVMLVNIR

QFCAIMAGDEPSLDAGAQRTQKGFVVRNMADWFGYLTYDVMGELCFGKSFGMLIERGKREVIGLVDRAAYRHYVCGLWMP

LDNWHLDQIFIRKLTNDRWNFIQKSRVEANQRAKERTQAGHEAKKDFFYYLLNAKDPESGRGLSTPELWGESNVLMIAGS

DTTSTSLAATIFYLVRTPDALAKLKKEVRDTFNDVEEIVTGPKLNELVYLRACMDEAMRLCPAVPGAMPREVLPGGIEVD

GLYLPGGIDIGTPCYAIHRKPEYYREPHTYIPERWIEGALCQSENGMWTSSKANVDLARRAFCPFSIGPRGCIGKGMALM

EMRLTLARMMFLFDIELADRVGEDATGHLHMVDHFTSQKNGPNIIIKKRQFA

 

>CYP548D6 Aspergillus clavatus

82% to CYP548D2, small deletion in lower case region

ACLA_098250

MFVHTLLANLSVGDVAVRVSFLLSALSIAWVVYQRFISQLCFIPGPFLASITPWVQLYHGLKGDRHLWLHRLHQKYGTHV

RVAPNFVSVNTARGLHDIYGHGKRLRKANFYNAFPAIKGVYNTHNAIDKTMHGRKRRVLSQAFSDHALKSMEDVMLLHVR

QLCaalaggsaagqka

VFNLGDYFSYLTYDVMGELCFGKSFDMLVSSGKRKLIELVDRAANRHYV (0)

CGLWMPLDTWHLDQI

FIHKLTNDRWNFIMNSRVEANARAKERAQAGREAKKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTTSTTLSA

TLFYLVRDPRALALLQKEVRDAFTSVEEIITGPQLNELVYLKACLDEAMRLAPAVPGAPPREILEGGASVDGVFLPAGTD

CGTPTYSVHRQAEYYPEPETYIPERWIEGATCKTATESWQVSKEAVEVARRAFCPFSIGPRGCIGKSMAFMEMRLTIARL

MFLFDFELADRRGEDEKGQLALVDHFTSAKYGPNVVVRKREM*

 

>CYP548D7 Aspergillus terreus

84% to 548D3

ATEG_07228.1

MIVQEIWGCISLPRLLLGVSVLLGILTSGYVIYNRFFHPLRRIKGPVLASVTPWVQLYHGLKGDRHLWLHRLHQEYGTHV

RVAPNFVSVNTAQGLHDIYGHGKRLKKANFYNAFPAIKGVYNTHNVIDKTVHGRKRRVLSQAFSDQALKSMEDVILLHVR

QLCAALAGPQADGHHAEEQKGTVQNIGDWFSYLTYDVMGELCFGKSFDMLVSSGRRKMIELVDRAANRHYVCGLWMPLDS

WHLDQIFIHRLTNDRWNFIMKSRVEANERAKERAQAGHDSKKDFFYYLLNAKDPETGKGLTTPELWGEANVLMIAGSDTT

STTLAATIFYLVRNPAALAKLKKEVREAFSTVEDIVSGAKLNELLYLKACLDEALRLAPAVPGAPPREVMEGGAVVDGVF

LPAGTDCGTPTLSIHRQPQYYREPGAYLPERWIEGATCRAPTGDEWQTTKEDVETARRAFCPFSIGPRGCIGKSMAFMEM

RLTIARLMFLYDLEKADGTGEDEEGHLALVDHFTSAKQGPNVIVRRRAE*

 

>CYP548D8 Aspergillus niger

fgenesh1_pg.C_scaffold_9000084|Aspni1

82% to CYP548D2

name typo: was labeled CYP584D3

MLAQIGWQALSVTRLGWGLLALLVGL (0)

NQSLIYATYNRFFHPL

RRIPGPLLASITPWVQLYHGLQGDRHLWLCALHEQYGSHVRVAPNFVSVNTAQGLHDIYG

HGKRLKKADFYNAFPAIKGVYNTHNVIDKVMHGRKRRVLSQAFSDHALKSMEDVMLLHVR

QLCAGLARAQQTGVVQDMGRWFSYLTYDVMGELCFGKSFDMLIESSRRRLIELVDRAANRHYV (0)

CGLWMPLDRWHLDQIFIHRLTNDRWNFIMNSRVEATKRAQE

RTKAGHDAKRDFFYYLLNARDPETGQGLTTPELWGEANVLMIAGSDTTSTTLAATIFYLV

RNPQAMAQLQKEVRENFTSVEEIVTGSKLNELVYLKACLDEALRLAPAVPGAPPREVMEG

GAVVDGVFLPGGTDCGTPTYSIHRQAAYYRQPGAYLPERWIEGAICQTGMEAWQTTREDV

EMARRAFCPFSIGPRGCIGKSMALMEMRLTIARLMFLFDMQLADRRGEDEEGHLALVDHF

TSAKEGPDVIVRARAV

 

>CYP548E1 Nectria haematococca

fgenesh1_pg.scaffold_5000761

Necha1/scaffold_5:2660663-2662388

76% to AAIM01001130.1 Gibberella moniliformis

44% to 548A4, 41% to 548A3, 36% to 548C1, 35% to 548D1

This gene model seems correct

MPFQLVAERVTPLQLLLLVSSLVLSYVVSVVIYRLYFHPLAKYPGPFWARISAFPAYIHTQRKDRHVWFWQLQQQY (1)

GPSFRITPNSVLINTPTGLRTIFNNKANVKKAEYYKAYPRNVNAMTTWNTIDKNVHARKRRVMN

NAFSDKALRSCEPFIHDNVDRWCELIIDEIGEGEKWSKSLNMARWADHLIFDILGDLCFGKSFGMKE

PGSELRYVPGLMTDFLEILHP (0)

IAYSPFTALWVWLKPRGLNQLLAAAAPPALKNWQSFVEKCFAERARVEHSAEKSEGGG

RTDFFHYLFKAVDPETGKGYSEDELFGESESLIIAGSDTTAITTAAAFFYLSRSPEVQQKLSDE

ITSTFSSIDEIKSGPTLHSCKYLRAFIEETLRMSPPVPADLSREVQKGGIVVEGEYIPEGIKVSTVSYCM

HHNPEYFPEPFKFQPERWIVDEDDTTGASAERLAVAQSAFMPFSAGPRGCIGKNLAYLEMNLVLAKIIYN

FEIRRDPASNLGGGSPKAIEGRQTVDQYQLYDIFVGIRDGPMVQLAKRTRTA*

 

>CYP548E2 Gibberella moniliformis

AAIM01001130.1

76% to fgenesh1_pg.scaffold_5000761, 42% to 548A4, 39% to 548A3

also Fusarium verticillioides FVEG_03837

1931 MAIINSVTVFSSSQIFWGIVF

1994 LSFCYVAAVVVYRLYFHPLAKYPGPFWARISAVPAYYYTLRQDRHVWFWQLQEKY (1)2158

2221 GPTFRITPNSVLINTPTGLKTLFNNKANVKKAEYYKTYPRNVHVMTTWNTIDKAMHARKR  2400

2401 RVMNHAFSDKALRSCEPFIHSNIDRWIELLDQEIGEKKWSPSLNMARWADHLIFDTLGE  2577

2578 LCFGKSFGMKEHDSELRHIPTLMTDFMSTIHP (0) 2673

2725 IAYSPFAPLWA  2757

2758 WLKPRGLDFLLAAAAPPAFSKWQAFVEQCFTERVQVENNMRGLWEKGESRKDFFHYLFQA  2937

2938 VDPDTGKGYSKDELFGESESLIIAGSDTSAISLAAAFFYLSRYPHIQQKLAEE  3096

3097 VKSAFSTVDDIKGGPALYSCQYLRAVIDETLRMSPPVPADLSREVQEGGILVDGEY 3264

3265 IPEGMKVSTASYCMHHNPDIYPEPFKFRPERWIVDEKSECGVSPESVSLAESAFMPFSAG 3444

3445 PRGCVGKNLAYLEISLALAKTVYHFEIRRDFSSNLGGGSPNAIEGRRTVDQYQLRDIFVA 3624

3625 IRDGPMVQLAKRS* 3666

 

>CYP548E2 Fusarium oxysporum (temp 548E4)

94% to CYP548E2 Gibberella moniliformis

FOXG_05969, supercontig_6

MAIINAVAILSSSQIIWGITCLLFCYIAAVVVYRLYFHPLAKYPGPFWARISAVPAYYYTLRQDRHVWFWQLQEKY (1)

GPAFRITPNSVLINTPTGLKTIFNNKANVKKAEYYKTYPRNVHVMTTWNTIDKTMHARKRRVMNHAFSDKALRSCEPF

IQSNIDRWIELLDQEIGEKKWSPSLNMARWADHLIFDTLGELCFGKSFGMKEHDSELRHIPTLMTDFMSTIHPIAYSPFA

PLWAWLKPRGLDYLLAEAAPPAFSKWQAFVEQCFTERVQAENEMRGLWEKGDGRKDFFHYLFQAVDPDTGKGYSKDELFG

ESESLIIAGSDTSAISLAASFFYLSRYPHVQQKLAREVKSVFSSVDDIKGGPALYSCQYLRAVIDETLRMSPPVPADLSR

EVQEGGILVDGEYIQQGMKVSTASYCMHHNPDIYPEPFRFRPERWIVDEKNEFGVSPESVSLAESAFMPFSAGPRGCVGK

NLAYLEMSLALAKIVYHFEIRRDFSSNLGGGSPDAIEGRHTVDQYQLRDIFVAIRDGPMVQLARRSRTD*

 

>CYP548E3P Fusarium oxysporum

pseudogene 69% to CYP548E2 Gibberella moniliformis

FOXG_14148

MTAMITDSSLQMILSGTVFVSTIYGAAIVWYRLYLHPPSKYPGPFWSWISA (&)

VPGYCYTSR*DWHMKYSKYYEIC (2)

LTFRITPNSVLINTPSGLK

TIYNNKANVKEAVCYKVYHCNVHVTTTWISIDNATHARKRRAMNHAFSDKALRSSEPFIH

SNIDRWIELLKEEIRKRQWSCSLHMARWADRLVFDTLGDLYFGESFGTKERDRELRHIPAIS (&)

VFTSTIHS (0)

IGYSPCTSLWAWLKPGLDYLLAAAAPPAMSKW

TFVEECFTKRAQIEDEMQARCKSGTESRKDFFQYLVLALDLETGKGCSNEEVFSESGSL

IIAGSDTSAIGLAAAFFYLTRNPHAQEKHAKESRAAFSSVGGIQGGAALHSSQYLRAFIQ

GTMRMSPPVPTDIAPEVQQ (?)

GGIVVGGRYIPEGTKASTVSYCMHHDPDMYPEPFKFRPERI

VDEKDECSDSPESIYLAESAFMTFSAGPRGCVGKNLAYLEMSLTLAKTVYHFKIRRDHSS

NIGGGSSGAINGRRIVDQYQL*

 

>CYP548E4 Fusarium oxysporum (temp 548E6)

86% to CYP548E2   Gibberella moniliformis

FOXG_14116, FOXG_12421 identical sequences

MAIVDTITAFPSSQIILGTAFLLLGYIAAIVPYRLYFHPLAKYPGPFWARISAFPAYYYTLRQDRHIWFWQLQEKYGPTF

RITPNSVLINTPTGLKTIYDNKANVKKAEYYKVYPRNVHAMTTWNSIDKTIHARKRRVMNHAFSDKALRSCEPFIHSNVD

RWIELLDREIGEKKWSSSLNMARWADHLVFDTLGELCFGKSFGMKEHDSELRHIPVLMTDFMATVHPIAYSPFAPLWAWL

KPRGLDYLLAAAAPPALSKWQMFVEKCFTERAQIENEARGVSKSGTESRKDFFHYLFHAVDPDTRKGYSNDELFGESESL

IIAGSDTSAISLAAAFFYLTRYPHAQEKLAKEVKSAFSSVDDIKGGPALYSCQYLRAFIDETMRMSPPVPADLAREVQEG

GIVVDGQYISEGMKISTASYCMHHNPDIYPEPFKFRPERWIVDEKDDCGVSPESVSLAKSAFMPFSAGPRGCVGKNLAYL

EMSLALAKIVYNFEIRLDHSSNL GGGSPDAIKGRRTVDQYQLRDIFVAIRDGPVVQLAKRTHMD*

 

>CYP548E5 Fusarium oxysporum (temp 548E7)

frameshift at C-term

76% to CYP548E1   Nectria haematococca

FOXG_07116

MFIRYTVKDISPSQALALVAALALSWIIATIVYRLYFHPLSKYPGPFWARISAFPAYYRTKKQNRHIWFWQLQQKYGSTF

RITPNTVLINTPTGLKAIFNNKANVKKAEYYKAYPRNVHAMTTWNTIDKTIHARKRRVMNNAFSDKAMRSCEPFIQENID

RWFELINEEIGKKQWSDSLNMARWSDHLVFDILGDLYFGKSFGMKEHDSDLRHIPRLMTDFMALLHPIAYSPFTALWVWL

KPRGLDQLLAVAAPPALSRWQNFVEKCFAERAKVEDDARKLNKPEADSRKDFFHYLLQAVDPVTGKGYTKDELFGESESL

IIAGSDTTATSTAAAFFYLSRSPQVQEKLVKEITSAFSSADDIKGGTTLYSCQYLRAFIDETLRMSPPVPADLAREVDKG

GIVVDGKYIPEGINVSCASYCLHHNPEFYPEPFKFYPERWIVDEKNESGVSAESVALAQSAFMPFSTGPRGCIGKNLAYL

EMSLVLARIVYNYEIRPDITSNL &

GGGSLNAVEGRRTSDQYQLHDIFVGIRDGPMVQLARRTRSA*

 

>CYP548E6 Mycosphaerella fijiensis

56% to CYP548E1   Nectria haematococca

e_gw1.5.510.1

MAWLFDSVLPSEGLVYPNILALAVAIITIAVSTTAIYRLYLHPLAKFPGPFWARLTTIPSYWHTHKGDRHVWLWRLQQQY

GTTFRYRPDAVLINTPTGYRTIFGPKGNVRKSDYYRIWPSDSSVSNAWNATEISIHGPKRRVLNHAFSEKALRSAEPFIQ

SNTDRWCELISNRIPKSNEWSPSLNMTKEINHLIFDILGDLCFGKSFEMKEPASKLRYVPELLADFLVLMHPIGFSPIAD

FWVWLKPRGLDALLAYTAPAALAGWENFVAKCLQDRTKVEEEVREGKLEARQDFFYYLFDAKDPETGKGYTRQELYNECE

LLIIAGSDTTSIVTSAMMFYMSRNLEIQAKLAKEVCGAFKSAGEIQSGLKMQSCRYLRAFIQEALRMCPPVPADLARQVR

AGGTTVEGHYFPEGTKLSTALYCLSYNEDVFPEPFSFKPERWILGESGGCATEESLALAESGFCSFSTGSRGCIGRNLAW

MEMMIVMAKVVWNFEIRQDEGNPLGAGDEKGPEGRRCVDQYQVKDAFVAMRDGPMIQFRRRTHE*

 

>CYP548F1 Aspergillus oryzae RIB40

AP007157.1d genomic DNA, SC023

complement(join(472501..473171,473240..473483,

473556..474019,474079..474229,475214..475264))

EST AB227122

42% to 548A4, 41% to 548A1

MQVGGLRSRVSVLVAIQLVSLIVYRIYFHPLSKIPGPLLPKITD

WYPAWCVWRGSSHTALWEGHRRY GSIGRCGPNSVSVCSQTGLMEIYSTKANVCKDESY

VVMSVGSHAPNTFSFIDKKTHAFRRKILFQAFTDNALNGVQDQILSHISEFCAMLNPP

PSNGAGQSSVWGPSVDIAPLCDYLAFDVISDLSYGRSFGMLKSDRYRYVPKLTRRLAR

RNATCMTQSKLWRYKLDRLFFAGFLKALRDFGLWIRHQGKERIRLGNNGPRKDCFHYL

LSGSDPKTGQGLTERELRVELLLLIVAG SDTVATSLSAVLFNLAHNQQALQKATAEIR

SCFEREEDIRLGTRLKSCSYLHACISESLRISPAVSNMPPRRVLPGGITVDGYYIPEG

TIIGTPIYALHHNEEYFPRPFKYEPERWLENEANGENPSTDDGLKRARAAFCPFSIGP

RSCVAKNLAWAELTLTLARVLFSYDVRLPPDHCEVEPDCCSSVPRDQSPEYKLRTWIV

SAREGPSLQFRPRNVKVP

 

>CYP548F1 Aspergillus flavus

100% to CYP548F1 Aspergillus oryzae

AFL2G_04070 revised

MQVGGLRSRVSVLVAIQ (0)

LVSLIVYRIYFHPLSKIPGPLLPKITDWYPAWCVWRGSSHTALWEGHRRY (1)

GSIGRCGPNSVSVCSQTGLMEIYSTKANVCKDESY

VVMSVGSHAPNTFSFIDKKTHAFRRKILFQAFTDNALNGVQDQILSHISEFCAMLNPP

PSNGAGQSSVWGPSVDIAPLCDYLAFDVISDLSYGRSFGMLKSDRYRYVPKLTRRLAR

RNATCMTQSKLWRYKLDRLFFAGFLKALRDFGLWIRHQGKERIRLGNNGPRKDCFHYL

LSGSDPKTGQGLTERELRVELLLLIVAGSDTVATSLSAVLFNLAHNQQALQKATAEIR

SCFEREEDIRLGTRLKSCSYLHACISESLRISPAVSNMPPRRVLPGGITVDGYYIPEG

TIIGTPIYALHHNEEYFPRPFKYEPERWLENEANGENPSTDDGLKRARAAFCPFSIGP

RSCVAKNLAWAELTLTLARVLFSYDVRLPPDHCEVEPDCCSSVPRDQSPEYKLRTWIV

SAREGPSLQFRPRNVKVP

 

 

 

>CYP548G1 Aspergillus clavatus NRRL 1

AAKD02000022.1 ctg02_1099423829560, whole genome

65% to 548G2P 39% to 548A3, 41% to 548A2, 42% to 548A4, 40% to 548A5

in the CYP53 clan part A

ACLA_043850 GENE MODEL REVISED AT N-TERM

MILAVHNLLDSLTVYSWRLLTTGASLGLFFL (0)

IGLSVYRLFFHPYAKYPGPLLAKLTSWYAVYHSYVGDLHTDIWRCHQKY (1)

GDVVRYGPNRIVIDSEYGLK (1)

AIYGHGANVHKSKGYERVSFIPKVHATL

CTLDDARHKFYRRLMNQGLSDTNIRRMDNKLKSIASLFASSLGEPTDRFHKSEDPAGDGWSVPKNMSHW (1)

CDYFTFDVMSELVFSKSYNLLTDSTNHWIIEGVLGQ

MQRFGFLLQLPELETLKLNHLFFPEARRRAIRFSAKAREIMQERQSRQKEELNDILGNLL

AAKDPETGEGLPNAQLWVDTNLLIIAG (1)

SDTSSTGMAALFFYLSRNPTAYDRVIKEVRAVFNNPEEVAQGPLLNSCIYL

RACISEAIRLCPAVSGALWREVLDGGLSIPEMNIHIPA GCEVGTGIWSLNHNEKYFPDPF

AFRPERWIAEESGDEAVTLAKSALASFSVGPRNCVGKGLAIIEISLAIAAVISQYDFRKA

ETRCTEVGEGKGEFKGQFQTSWAFTSLKDGPYIQFRKYMPGSN* 306652

 

>CYP548G2P old name = CYP668A1 Aspergillus nidulans AN1300.2 AACD01000017

poor match 53 clan similar to 548A3

pseudogene of CYP548 family

45% to 548A1, 40% to 548A3, 41% to 548A2

65% to AAKD02000022.1 Aspergillus clavatus

344602  FFYLSRNPSAYDR

VTKEVRNAFLTPDSVCQGPKLSSCVYLRACIQEVLRLAPAVSGALW

REVLPGGLRI  344397

IPA

344356 GTGIWTINHN

ERYYPQPFSFRPERWIPEEFGEEAVQLATAAFTTFSIRPWNCVGKALAMI

EMTLAMAAVISHFDFRRADGAAGGLGEGKGSFDGQFQTFWAFTGFKDGPM

IQFRPRKY* 344006

 

>CYP548G3 Fusarium oxysporum

58% to CYP548G1

FOXG_16410 revised

MALSTNSPVPSWTLFASSAGLGIL (0)

LLYICIYRVFFHPYAKYPGPFLAKLTSWYSVYHTYYGDLHLDIWECHQKY (1)

GNYVRYAPNRVLINTPEGLK (1)

AIYNHPKNTQKSKAYRK

MSLVPGVHSSFSTIDNQAHAKLKSLISQGLSNSHIRAYDPELRQSALLFATRLGEKIDRFEPDQSDVGEDGWTAPKNLAS

WSNYFTFDVMSHLVFGTSYNLLTNSENHWVIDGVLGQMRRMSFLTMLPELEDMRFDLLLFSNARRMAYRFAVKSREIMEA

RKIREKAMEGQETKVDLFSKLLAAKDPETGEGLPDKQLWAESNVMIIAGSDTSSTAIAATLFYLSRNPSAYARVTKEVRS

AIITPEDICQGPKLLSCTYLRACIFESIRLSPPAGGAMWREVMPGGMHIAGNDVDIHVPGGCEISTGIYAVHHNEEYYPD

PFEFRPERWLADEVGDETVAKAHAAFDAFSQGPRGCPGRSLALIEISFAVAAVIMSYDFRKVESSLGEVGEGNGKFAGQY

QTSWVFSSFKDGPYLQFKRIK*

 

>CYP548H1  Aspergillus oryzae

          GenEMBL AP007171.1 BAE64607.1

          45% to 548C1, 50% to 548B1

          14 P450 genes and 2 pseudogenes on this contig

MQAVALLRHVPLFAYVTAVPVALLAYVLAICIYRIWFHPLAKYPGPLLAKVTNLYGGYYAWKGDLHIDMM

RCHEKYGNYVRYAPNRVLFNTNTGLKEIYAYSKSFQKSAAYGAMVHRAPNTLTLIDKKQHGRKRRIIGQG

FGDAALRGFEGTIMSLVRKFCDELAKDISGRKAGEWSTPQNMGKWSNYLTFDIMSGIIFGESFDLIGSPK

NREIVKCIEDSNVRTGVLSQAGELSTRRLDRWLFPQAIQGRNAFIRFVNILLKKRMSAKPLKRHDAFSFL

LDAVDPETQQGFTPAEIGAESTTMIVAGSDTSSTAIASTFFYLCRNREWYEKAKEEVRAAFPGPDDVALG

PALNNCVILRACIDESLRMSPPASSSLWREVLDDGVIIDGQVVPRGYDVGTCIYAIQHNPDYYPEPFEYR

PDRWLDPVPEKVQLARSAFSPFSIGPRSCLGKGLAWTELMLTMAYMLSKYEFRSAPGEDEKVGGGHVDMG

AGRQREGEYQLRDHVTAAKDGPIVQVSFR

 

>CYP548H2 Aspergillus niger

gw1.11.642.1|Aspni1

56% to CYP548H1

VYAIYHLFFHPLAHFPGPFWAKFTCLYSGYHAWKGDLHIDMQRCHEKYGDFVRYAPNQLLINTSIGLHDIYGFGRNTQKS

KLYSAMVHRAPNTLTLIDKKAHGRKRRIISQGLSDAALRRYQPAILKHINELCGALVGSKPSEWSVPRNVAHYFNFLTFD

IMSDVLFGEHYNMVTSEEHRYVVKAIEDSNVRTSVLAQAPLLAFRRWDRKIFAESILARNKFIQFVNTLLQRRFKAAKSA

RQDVFTFLLDAKDPETQQSLSLAEIGAETTTLVVAGSDTSSTAFSSCLFYLSHNKDWYNRVATEVRNTFSSPDEITLGPR

LSSCIYLRACIDESLRMSPPAGSALWREVGPGGATFDGHFIPAGCDVGTGIYAIHHNENYYPEAFNFRPERWILAGAEGD

TEAVGMADRDSLAKAQGAFNPFSIGPRGCIGKGLALAELTLGIATMLWRYDFALGEGKGMALGAGRKGLGPGRERENEYQ

LYDHITASKEGPMVCFRER

 

>CYP548J1 Gibberella moniliformis 7600 chromosome 11 cont3.130

26kb from a PKS

AAIM02000130 revised

Also Fusarium verticillioides, FVEG_10545

45% to CYP548H1, new subfamily in CYP548 CYP5115 may belong here too

41% to DR630735 DR651021.1 DR621844.1 Gibberella moniliformis cDNA

44% to XM_001273103.1 Aspergillus clavatus

43% to XM_001402423.1 Aspergillus niger

      MPMSHMKTHWLPYAIRISRSITPPPLLIILLLAALSP (0)

      VIGYVVYYAFFHPLAKYPGPFWGKFTGLRAAYHAWIGDVHIDMWKCHEKY

      GPYVRYSPNYLLMNTTQAYK

57293 DVYGHNKNVRKSLAYLAMVHKTPSTFTLMDRHEHAWKKRILSQKLSDSAIRSFEPKITGM 57114

57113 IDRFCQYVCPPASTGKENTVSKPFNMSEMC (1) 57024

56947 DYLFFDLVTSIVFGENFDLIRSPWYRHIPPALARSNERISVIVQWPYVVWRRMDKVLFRN 56768

56767 SVAGRKDFLRFAHNLVTERVQRGSGDDVLSGLLDAADPTTGNKLTQDEIVAESILML 56597

56596 VA ()

      GSDTSSTLLASLFFYLTRNPDKKDRLTREVRSKFSTREEICLG 56417

56416 PALNSCRFLYACIMECLRLTPPVAAAPFREVLKGGMIVDGHYVPEGTNVGTGLFSIQHNQ 56237

56236 EYFYSPFEFIPERWLSEGKNAGPHNPDAHVPFSIGPRVCLGRALAHAELSL 56084

56083 AMAIICWKMDFNVVESMKDIGAGNENAEYGRHRPGEFQLYDHITCARNGPMVEFRERSF* 55904

 

>CYP548J1 ortholog Fusarium oxysporum

97% to CYP548J1 Gibberella moniliformis

FOXG_11965

MPMSHMKTHWLPYAIRISRSITPPPLLIILLLAALSP (0)

VIGYVVYYAFFHPLAKYPGPFWGKFTGLRAAYHAWIGDVHIDMWKCHEKY (1)

GPYVRYSPNYLLMNTSQAYK (1)

DVYGHNKNVRKSLAYLA

MVHKTPSTFTLMDRHEHAWKKRILSQKLSDSAIRSFEPKITGMIDRFCEYVCPVSAGKENTVSKPFNMSEM (1)

CDYLFFDLV

TSIVFGENFDLIRSPWYRHIPPALARSNERISVIVQWPYVVWRRMDKVLFRNSVAGRKDFLRFAHNLVTERVQRGSGDDV

LSGLLDAADPTTGNKLTQDEIVAESILMLVAGSDTSSTLLASLFFYLTRNPDKKDRLTREVRSKFSTREEICLGPALNSC

RFLYACIMECLRLTPPVAAAPFREVLKGGMIVDGHYVPEGTNVGTGIFSLQHNQEYFHNPFEFMPERWLSEGKHAGLHNP

DAHVPFSIGPRVCLGRALAHAELSLAMAIICWKMDFNVVESMKDIGAGNENAEYGRHRPGEFQLYDHITCARNGPMVEFRERSF*

 

>CYP548K1 old name = CYP5115A1  Phaeosphaeria nodorum SN15

           GenEMBL AAGI01000308.1

           47% to 548H1, 39% to 5114A1

           37% to 5114A1, 39% to 548A3, 37% to 548G1, 38% to 548A6, 34% to 548D2

       MNYLSVLVVAVAVTLLY (0)

43655  LIARVIYLAFFHPLAKYPGPFLSKFTCARASYYAWRGDIHIDIWRCHEKYGDYMRYGPNQL  43473

43472  YVNTPKGLRDVYGPTTSNKFLKSSHYEVMTHQAANTFTHRGGKEHLRRRRIMAQAVSTKA  43293

43292  QLEYEPRLVGHIQKFCNAVFKGEPMNMAKWCNYLAFDSMTDVVFGAQYNL  43143

43142  LGDEKFRYVPEMIEKSNVRISSLVQFPGLTWLRLDKHLFQEAIYARNRFLKFVFRLLSDR  42963

42962  RALSHGTARGVFENPSISLDVYSRLQDARDPVTGEGFGHDEIASESTTLIVAGSDTSACA  42783

42782  TASILFYLANNPHAYARVAEEVRAVRSSRPGHLAAADLAGCKYLRACIDEALRMSPPVGT  42603

42602  GLMREVAAPGGLVVDGHVVPMGCEVGVGTYAIHHSTRAFEDPFVYRPERWLAPDESAHAR  42423

42422  ACFVPFSAGIRSCLGKGLAYTEVTLTIGHLLWLGDFKLVADAELAAVGRGSRK  42264

42263  LGVDGRHREGEYQLYDHIAGQKNGPWLQFTRRAEVQ*  42153

 

>CYP548L1 Aspergillus niger

gw1.12.692.1|Aspni1

51% to CYP548K1

VIYLLFLHPLAKFPGPIFASLTHGYGAYHSWKGDAHLDMWRLHEKHGDFVRYMPNSVFIRNADALHAIYSTKAYASVVKS

PHYEVLQAGSVGSTFSLRGGHEHLRRRRIVSTALSEKAQRGMDPRIAVHVRKFCEAVLPARQGELGEPMNMAEWCGYLTF

DLMSDLVFSASYNLLGREKFRYMPEVIDKSNVRMSVLAYMPILAAWRAVDGIFFRDALIARNRFLRFVVRAVRERANRAL

GKWAPDALDPCKPRDDIYSALATAKDPDTGEGFKPSEMVSESTTLVVAGSDTTSTAIAATLFYLADNRFAYEKAAQEVRT

VFAGKSLGSEEALSGPELSQCTYLRACIDEALRMSPPNGAALTREVLPGGLTVGDIHIPGGVTVAVPLYAIHHDERYYVD

PFTYRPERWLEDDGTGSIKRARLVFNPFSLGMRGCLGRSLAYHELMTTVATVLYLSDFEFAEGPMSKVGRGVPGAVYGRH

RENEYQLREHITGQKDGPWLRFSRRE

 

>CYP548M1 Aspergillus niger

e_gw1.8.742.1|Aspni1

67% to fgenesh1_pg.C_scaffold_4000313, 42% to CYP548A2

MDLLGCVVIAVAGYIIYQVWFHPLSSYPGPFVAKLTNLYSVVHAIRGDRHEDLYHLHQRYGPVVRVGPRRVSILDAKALE

PIYGFHANVQKAKSYNIFYGVSIFNAIDRTVHARKRRVMSHAFSSQALRGMEPHILSAIRDWCAALGDQHPDKQVLSACS

RPGSWSRPKNMVHWSACVVFDALGEICFGKTFNTSFSDANHFFFPLMALNLRIMNICGQMPILCSLGLERYLRRGTVANR

QRQIDFSRQQLQTRLAAESTQRRDIIFYLQQARDPKTNEGYSEAELMSEVMTLLGAGNDTTNTTLTAIFYYLAHNPAILA

RLSAEIRAVFPTLEAIVAGPDLSQMAYLHACIDETMRVCPPVPTDLPREVLPGGLRVGEWYFPAGTVVAVPTYALHHSED

HFYRAFVYDPSRWLLCGSKGTEQGEGVSAEVLSRQRQAFAPFSMGPRACIGRNVAILELELTISRALWLYDIRLAPGTEQ

LGVGYQGEYKIKDHFAVGKEGPVLQFRMRQK*

 

>CYP548M2 Aspergillus niger

fgenesh1_pg.C_scaffold_4000313|Aspni1

67% to CYP548M1, 44% to CYP548A1

MLDNFIQLVLGTISLGKLLFAILSAAGLSYVVYQVQFHPLAAYPGPFLAKLTNLYSVVHAMRGDRHEDLYKLHQRHGRIV

RIGPNRVTILDARALEPIYGHQANVQKSQWYHNFYSVSIFNAIDRNVHARKRRVMSQAFSDHALRGMEPHILSAIRDWCL

ALGDEHSNQSQGKQSQASSSSSSSSGGGWSRPKDMVHWSACLIFDALGEICFGKTFNTTRSDENTFFFRLMAVNVRILNI

CGQMPLLRRIGFDQYLRMGTAGDRERQIAFSRQQLSTRLAANPTERRDIIYYLQQARDPETGEGYSTQELISESTLLLGA

GAETANTALASTWFFLAHHPAIRQRLTNLIRAHFASLESIVSGPALSSGKMGYLRACIEESMRLCPPVPMDLPREVLPGG

LQVLEWEFPAGTIVGVPTYSLHHSPEHFDRPYEYDPNRWMLRGTEAADEGVGVSPEVMARQRAAFVPFSLGPRACIGRNV

AMLELEVSVARVLWLYDLRLAPGMEHLGVGREGEYKMKDNFIVGKEGPILEFRKRA*

 

>CYP548N1 Mgr037 Mycosphaerella graminicola

48% to 548A6 N-term from JGI model e_gw1.10.623.1|Mycgr3

MDQSSSVPDQIFLRPHQENIYTLLNSSHYTWTILALTTLLTALT

SYTIYQLHFHPLASHPGPLLGRLTQLYDLYHAYIGDKHILFHHLHQKYGPIVRFSPNSLSINDPAALKTIYSHGANVQKS

VFYKCFRAAPQAISTLLATEKQHHARKRRIMGQAFSEAALRGFEAFVVGHVEDLVGRIGEVVGSSSSSSRGQEGEKWSAP

LDMANWCNWLVFDIMGDLVFGRSFGTLGEKPENRRGIFLLGRAARRNYTIAAMPMLLYTGLEKVLPILRGLYLDRCQYLA

FGKKQVMERTKE GAGSAEPARRDIFSFLLHAKDPEEGEGMPMPELWMEGNTLIVAGSDTTSTTLAAALHYLLHNPHTLHR

LESEILSTFSSPSEIAMGPKLQSCTYLRAVLDETLRMTPAVGGLLPREVLPDGLSIPSLDLHLPAGVDVGVPIYAIHHHK

DYVVDPFVFDPERWIREDHAQDREKLMSVFNPFSIGHRGCLGKPLVYMELGIAVARLVWEFEMRLSEVQRVSGFVERDVR

RGRRVEGEYHLLDWFMSRNEGPRVEFRRRE

 

>CYP548N2 Mycosphaerella fijiensis

73% to CYP548N1 Mycosphaerella graminicola

estExt_Genewise1.C_160063

MELRPHLHIHTCASSYQCISAVALCGA

MLAVLTAYVIYQLHFHPLAKYPGPLLGKLTQWYDVYHAYVGDKHILFYHLHQKYGTVVRFSPNSLSINDPAALKVIYAHG

ANVQKSVFYKCFRAAPTAISTLLATEKGHHARKRRIMGQAFSDSALKGFEEYVVALTDNLVTRLQAAITRSDGEKTRWSA

PLDMAAWCNWLVFDIMGELVFGRSFGTLGEKPENRRGIFLLGRAARRNYVVAAMPALLYTGLEKWLPLLRGLFLDRCQYL

AFGKKQVMERTKEQSFGQPGSPSRDTGRRDIFSFLMTAKDPETGEGMPMPELWMEGNTLIVAGSDTTSTTLSAVLYYLLH

NPHTLQKLQREILTTFESSSEIRMGPKLQSCTYLRACIDETMRMTPAVGGLLPREVLPGGLEIPSLNLALPAGIDVGVPI

YAIHHHQDYIASPFLFDPSRWLPSDGKPHAQSKEALLAVYNPFSIGHRACLGKPLVYMELMISIAKIVWEFEMRLAEEQF

VSREIRKEVRSGKRQRYEYQLQDWFMSRNEGPWVEFKVREGAS*

 

>CYP548P1 Yarrowia lipolytica

45% to CYP548A1   N. crassa

CAG83449.1

MSLLETIVQFVTLVAAFVGGFLALAAFYRLTLHPLAQYPGPLLCKLSSIPDVYHAWKGDRHLHYYRLHRKYGSVVRYAPN

KVSFDTGAALKHIYSGNKKEFIKSDFYEGFPAVKGFPSVHSAIIKSEHAKKKRVMSHAFSEQALASAQPYIMDKIVQLCD

LLGDNTRDTVVTPSGKPAKMISRLTSYFSFDVMGELCFGKGFDMLTSADNRFALRMTSNAAHRHLVYGCFRLLDKLPFIG

SYFFGHIVEDRKKFVEYSRAVASKRMALGDEGHRKDLFHYLMHATDPETGKGFERNELLSESNLLIVAGSDTTATSLAGC

LHYLGLHPDVRKELTRQVRAQFYSVDEIHSGSTLTKGIPLLRACIDESMRLSPPVPGVLPRTALEDVVIDGCKIPKGCDV

GVSTWSMHRNPKHFVKPLEYDPYRWLRGGQVDDSSKYSDVAQDIDEKAALNETANARAGYAPFSIGTRSCIGRALAYSEL

DCAVARICFMYDFEVCLEGKLGSGVFGYGYDDSDEFVAGRGKGGKINEWDVKKRDGEEIGTFRLWDHFAGEKEGSMMVFA

HAKKE

 

>CYP548Q1 Coccidioides immitis

43% to CYP548H2

CIMG_05501.2 gc boundary at QAHQ?

MLSGRNR (1)

AVIIYELWLHPLAKYPGPLIAKFTHAHAGYHGWKGDIAHDIWRCHQKYGDYVRYAPNALLINTAQAHQ (1)

DIYGFGGKVVKAANYSILAQRAPSILTLRDAKEHRRRRHRVALALGEDNVRFYEPQMLNHIRTFCA

KMVEGVKNVDGWSALRNMAPWCNYLLFDIMMSMVFSSDSKLLTHPKYRHIPNAIHQSSVLQGVLVQDKTLAILGLHKYLF

KTAVRERVKFVHFIRTVLGIRLNKSASYTADSDPCHDVFAYLRNNLSSADLAQLSPKELSAECATLIIA (1)

GSDTSTMGLAGALFYLSRYRSA

YDRLVQEIREKFNDASDIRLGKTLYSCSYLKSCIQETLRISPPVGTALWREVEPENGATIDGAVIPKGTHVGVGIYSIHH

KPNHFSDPFTFRPE RWLSEARPADLVNHE TRAYGPFSIGPRSCVGKGIAMQELMLSLA

HIFWSLDFEALAPDGNRVTIEGSVLHPSPGWTEFPMKEYIIAVGDGPMLRFRRRAVE

 

>CYP548R1 Histoplasma capsulatum G217B teleomorph: Ajellomyces capsulatus

45% to CYP548A2

HCB01307.1

MDTLFSLASQDGVNGLLAVFAVCSIVGIYRLTLHPLAKYPGPFSCKLSDWATLFNASSGDRHLREWKAHQKYGPIVRTGP

NTVSINSVAGFKAIHGSRLSNVRKSDWYLTVEGAVGTPMTQTILDRQTHAFHRRVLDVALSETALKSVEHMIIENVHSWC

AHLAEGISKPGDWSSPKNMADWATYLSYDIMGDLTFGKKFHCMDSDEHRFVPLLMLNATKMVYFIGLSPLAPLLRPFLGS

PIMNVVGGQMARDHLKYKRYSASQMKQRIEAETDDSKPTRKDLAHYLLNARDSQTGKGLTTEELYSESSLLIAAGSDTTS

IAISAIMFHLSHHPEILGKLTSIIRSTFSSLDEIQAKALNNLPYLRACLDEGMRLSPVVPSHLPRQVLPGGLTIEKHHFP

AGIVVNTPPYTINHNEEYYPDPFSYRPERWIVDPYALDGDIGSESQVAIARSAFTTFGLGPRGCAGKRLAYLELGHAFTR

LLFSYDMRVSPANETLGAGNPNHEHEGRRRPDEYQFEDYFLVTRDGPLLEFRPRCE

 

>CYP548S1 Aspergillus clavatus

45% to CYP548A2 Magnaporthe grisea

ACLA_062280

MALSPSAIAGATALFGVVYLVVVSVYRLYFHPLARYPGPWLAKVTSLYAAYLSYTGEIHLEMWRAHEKYGDVVRFGPNRL

LFRTSTALKDIYGFGQNIRKAAAYHQFPTKFAYSTHNAVDKEMHRRKRKLVNQAFSKNALKIWETSQLQQIRIMCNELIQ

PSSTPSLSEKQGWSSPKKMSDYALYLMSDIVSEAVFGTSFRTLTEQTNRHVLHLVHKSYEWIGIIFQSPTISKLGLDGYL

IPQLARARQALRDDFRVLMRDRINSQTEKRDIFSIYSEYTDEKTGESFPLSEITSEALLLFGAGPDATSTSLTAAFFYLA

RNKAAYDKVVAEVLSTFTDVEEIVGGPKLNSCHYLHACIDEALRMNPVNGGALWREVVSGGATIDGHSIPAGADVGVAIY

SIHHQADYYPDPFTYRPERWIASPETGVTPAAVDAARKAWSPFSIGPRGCVGKSLATTLLHISLARVLFLYEMQLPKDER

LARVGEGKPGLPFPRNLTSELQSKGTFSSIVHGPMLEFKRR*

 

>CYP548T1 Neosartorya fischeri

43% to 548E1

NFIA_095340

Note: this seq does not have an ortholog in A. fumigatus

MFVAAGLGMMVAHWPAVIIGFAICYLLGSAIYNLYFSPLAKYPGPLFAKVSALPDFYWSLTGDRHMWIARNHEIYGDVVR

FRPDGLLFRTPQAHRDIFNHKANVKRAKFYDMLTRNKHDTSTITGTDPTLHAQKRRVLNTVFSEKSLRSMEPFLVKHVTR

WCELLIDGDGTDWSTPRKMSEACDYLVLDVLCDLCFGQSVDTKEPGENEYHKIPHAIGSFLDILYPLGHSPWLDLIVWLK

PRGLDWVFGALTPPHIQFLYEFVKQSLAQRLKTEKETPSTGQEGRGDMLHYLIHAKDPATGRVGYTPEALEAEALMLTIA

GSDTTSVIMAGFFFYIVRTPRAYRKLVNEIRSTFTNIDEIRAGPKLTSCQYLRACVDEAMRIMPPGPSEMPRIVLPGGII

VDGDHIPEGTTVGVASWSSYRNEEYFPDPNVYRPERWIVDEEAGVTAEDVARARSSCFPFAAGTTGCAGKNFALLELYMT

IASTLWQYDARLLPGDMTGAGSKAQGWGKRNPKIFHVKDKYISVRDGPMVQFKKRVA*

 

>CYP548T1 Aspergillus terreus NIH2624

AAJN01000173.1

92% to CYP548T1 Neosartorya fischeri = ortholog

ATEG_06914.1

21150  MYIAAVLTMMRPHWAAVIIGLAVCY (0)

       FIGSAIYNLYFSPLAKYPG  21329

21330  PFLSKISALPDFYWSLTGDRHMWIARNHEIYGDVVRFRPDGLLFRTPQAHRDIFNAKANV  21509

21510  KRAKFYDMMTRNKHDTSTITGTDPALHAQKRRILNTVFSEKSLRSMEPFLVKHVARWCEL  21689

21690  LIDGDGTDWSTPRKMSEACDYLVLDVLCDLCFGQSVHTKEPGENEYRNIPHAIGSFLQVLYP (0) 21875

       LGHSPWLDLIVWLKPRGLDWVFGALTPPHIQFLYDFVKQ  22049

22050  SLAQRLTAEQETPSSGHEGRGDMLHYLIHAKDPATGRVGYTPEALEAEALMLTIAGSDTT  22229

22230  SVIMAGFFFYIVRTPRAYRKLVHEIRSTFASVDEIRGGPKLTSCQYLRACVDEAMRIMPP  22409

22410  GPSEMPRIVLPGGIIVDGDHIPEGTTVGVASWSFYRNEEYFPDPNVYRPERWIVDEHAGV  22589

22590  TAEDVARARSSCLPFAAGTTGCAGKNFALLELYMTIASTLWQYDARLLPGDPTGAGSKAQ  22769

22770  GWGKRNRNIYHVKDKYISVRDGPMVQFKKRVA*  22868

 

>CYP548U1 Aspergillus terreus

41% to CYP548A6 Botryotinia fuckeliana

ATEG_03468.1 N-term exon not identified

FVTVVVYRLFFHPLSKYPGPFFWRISILPSLYYAWTGDRHLVVDKLHKKY (1)

GKVLRMEPNLISICTPNAIK (1)

TMYGPGTKFEKAMFYTRGPKEKQLLV (0)

NLASTTDKAVHARKRRIISHALSEASIRSYEVTILDKIQLFCKQLSDASTFGGPY

KNMSRWFSYLTYDIMGQLTFSQSYDMLTKDDHHFIQPLIDSYQHSQ (0)

LGTEPKLDQWGLAPLLLLRIMAENKKFRRYVDDQ

VNHRIALEKAGQGPPDIFKLLLEHKDKETGESMGFKELSDEAVVLIIAG

SDTTGTALSGLYFYLARYPECYAKLSHEIRSTFTQVDEIVGGPKLQACSYMRACVEEALR

MSPGVPGFLTREAPSGASIDGYYFAPNVQVAIPTWTMHRTPEVYPDPQVFKPERWM

VESEAELQRLRSSYYPFSMGTRGCIGKNMAYHTIYLTVARLVFL

FEIESRDELPLEFHVKDHFAAGSKDGPYLKFTRREL*

 

>CYP549A1 N. crassa AABX01000759.1 NCU01573.1 cont3.66 (version3)

39539 MLIALLAPFSLLIAYVLHHVLSHQTLLLKQIFSQTIFNTIQLARLIGPLD

PYDPLTVPDQVIPDSNSASEISEAQARANQRIDDARSIIDFSYAGPKIEL

EDRLRLRALANSRLVAAFGINTSLTSSSVSVHKKFRKLASASINKSRADW

QKLYGVCMDFLQREKARYGRTGIGLAECVRCLCFVVVLVSQFGVDDKKVD

KNLVKRITDGINAQWLKSKEDGEAVKTSDSLNHDLGMLFGNLKTSVAEEN

GTGKDTSIDPSEALGLIMPQYETLWRVVLLTYVTAYYTQYDKRSLKKRVK

DIPSCLGNAATEKEALKLAT (0) 38580

38519 EGLRLYPSNNSIYRAATGPGPLKSADVQAC

HRDFNVWGRDALEFRPERFDNLTPLQEKAYFPFSLGSHKCPAFGGFGNRM

VTMLVVSMGRALSPETGKLNFEDAQLDNRVGIILPTGRDEMENWSWDMVR

V* 38124

 

>CYP550A1 N. crassa AABX01000727.1 cont3.288 NCU05128.1 (version3)

125984 MTVENSIVQHALTVYHDLSTATKATLAISIAFILYRLLFRHESTRRKELP

AWGPIEMGLVMYLLDGARNGIVYRVY (2) 125757

125699 SAIRRYGGSFYGISSANQTLIGYE

DVDRLFSSQMNHALSIEWHGYGLFLRFFGFPDTPTLKKKVESTFKPWHSP

IERVFNNDAGATAAFERGNLPKIVSEFVSFSQEKEKMKRWELRADIKVIK

QGEAVEANLSALIMDFGACFTIPPIFGHDLLDRNPHLIEDIWKFDHVVPL

LFINMPTWAPFKVMKEGIEARKRLLAGVEAFSRRVTQFQRGEKVDDGADI

SDVSDVVLERNTALERNEWSYQERAASELLVLFASNTNTQPVLFWLILYI

YSTPGLVNTLRQEVAPFITLSDSHHDKKGITAINTSGLSRECQLLKSIIL

ETLRLAFGAVSTRFVKRPITVQDGNHNHKLYPGTFLSVPHSFFQRDPSLY

PDPHKFVPDRFLETDPSSGKLVAKYGKLRPWGAGASMCRGRVFAEKELMA

IGAALVTLWDISPVGGGEWTVPEMVGGPGPVKPKEEVRVVIKRRVFGGVS

SSE* 124263

 

>CYP550B1 Fusarium graminearum FG05960.1 AACM01000237 FGcontig1.237_scaffold3

MVQLSALYQNAYATFASLSPVYKIIFTIVVIFVLRKAYGGQGKAY

KNLPAYAPIELAIASYVLSGDGISRRVF (2)

SSAISRYGGSLFGITSGHQIV

ADLPGIERLLTQSHHIFDSTPAQYSLCTLVFGSTNSPQLRDKLHTSLKDL

VPPLERTFLNDAASTAAVERSRVAERGASLVTFSSDPQHMERWELSAGIK

VLQSEQPGIPGKVEANLQSLTRDFGACMSIPLIYGQDLLDRHPQLLDDFW

VFDNELFPLLMVGIPTWAPFKMMQDGLKARTRIIDSLEGVYKRVEQERRG

ESIDFGADISDVSTMLRERNKVFNRDGWTLKERAAADLGTFWGLNANAQP

VLFWFLLYVYSTPGLLERIRQEISPYVTLVGGGRPEIASMDFQSLFRNCP

LFKACIFETYRLVNEPTSIRHVSQSVSINDGDLLHNLSEGTYISVPLALR

NKDPHLYQDPDSFVPDRFLDTDPQTGKSTARYGKLKPWGVGAAMCKGRTF

AEKEIISLGSAVVSLWDIAPADGSWKLPAMIPGTGVKKPINDIRVVIKRR

VL*

 

>CYP550B2 Aspergillus nidulans AN9214.1 55% to 550B1 7 clan

revised 7/19/07

MDCISQNSVIQRALLALYEHPVAYSVI

TAVFISVLCRKLLYKPRNYALFPVWATIEVAIASYLLRGDGIGRRVF (2)

SVIRRYGGSLFGITSTHQILVDFPGLDRFMARSLHTLNAEPVQYTIFTRTFGG

VDSPELKRKLKNSWKDLLAPIERLFLNDASAAAALDRACVLQQAASFVSF

SSSPAQMKRWELSAGIRVIPPAESGSPHKVEANLQSLTRDFGACMAIPLL

YGRHFLDGNPTLLDDFWKFDNELFPLLMIGVPEWTPLRIVKDGCAARARI

LRELEALYRRIDQSQCGEPVESGIDMSDVSGALFERSRIYKREGWSFPER

AAGDFAIFWGQNANTHPLLFWFLAYIYSTPGLLDTLRAEIAPYTCFASSQ

AKVPEITSIDFPGLSANCQLLKACLYETYRLVNEPISIRYVERPVTLTDG

SLQHTLKTGTWVSAAHSLTQHNASIFDNPAEFRPERFLETDQVSGKRVAR

YGRLRPWGAGAAMCKGRTFAEKELTSAAASIVSLWDIEPAYGQVWKLPGM

VPGTGVKRPVRDIRVLISRRQSIVVKGNDMSPSKGENHGKPLGV*

 

>CYP550B3 Nectria haematococca

e_gw1.2.919.1

Necha1/scaffold_2:2281734-2283431

MSNSTPTQALRHSLSACISFNVACTIATALALALLLRKFMFRPKTGSIFPVWAMIELALVSYIFRGDGIGQRIF (2)

LAARKFGGSLFGLTSKHQVLVHLPDVDRLMEQSLHALSAEPVQYALLTRVYGGVDSPALKKKLENS

WRDFLPSMERLFLRESGATATIERARVAERAASLVTFSSDVQNLKRWELCADVRAIATDSQSSLAVVEAN

LYNLVRDFGACIAIPLLYGNDLLRQHPRLLEDLWKFDNDLFIPLLLGVPTWVPFKMIQEGLRARSRLLSA

MESLYRCIDQHQRGEVFNSEFDLSDVSSIPFERNKIYEREGWSLQERAAGDFSILWGQNANLQPMLAWLT

TFVYATPGLVEGIREETAAYINLSTIAPLEIISMDIPGLCRDCQLLKACIFETYRIANDPAVIRRVERPI

AIQDGELEHQLQAGSFVSMPTSLTNNDPAIFAEPDKFIPERFLERNPTIGNMTARYGRLKPWGMGSSMCK

GRTFAHKHLTVVGAAIISLWDIEPVSGAWEVPDMIPGSSAKRPAKDIRVVIKRRIL*

 

>CYP550B4 Fusarium oxysporum

78% to 550B1

FOXG_07659

MELSSLYQTASATFGSLPVAYKFLCAVLVIFAFSKAF

SGAERKGYKKNLPRYAPIELAIASYILSGDGIGRRIF (2)

SALSRYGGSLFGLTSGHQIFVDLPGVERLL

AQSHHTFNSDPAQYSLCTLVFGSTDTPELRAKFDTSLKDLVPPLERTFLNDAASTANVEK

SRVAERGASLVTFSSNPKDMARWELSAGIKVLQPETLDSPGKVEANLQSLTRDFGAC

MSVPLIYGQDILDRNPRLLDDFWTFDNDLFPLLMVGVPKWAPFRIMQDGLKARARIIDSLEAVYRRVDQYEKGEPVDLGA

DMSDVGMMLRERNKIYNRDGWTFPERAAADLGTFWGLNANAQPVLFWFLLYVYSTPGLLKRIRDEISPYVTLSDGAKPEI

KSMDFPSLFRKCQLFKACIFETYRLVNEPTSIRYVTQPVSLNDGNIKHDLQEGTYISVPLALKNKNPSIYEDPDTFIPDR

FLDTDPETGKATARYGKLKPWGLGAAMCKGRTFAEKEIISLGSAVVSLWDISPAEGEWKLPAMIPGTGVKRPVSDIRVVI*

SRRSPLKS*

 

>CYP550B4 Fusarium verticillioides

93% to CYP550B4 Fusarium oxysporum

no gene model

MELSPIYKNALATLGSLPLAYKVLCAVLVIFAFSKAFSGAERNGYKKNLPKYAPIELAIA

SYILSGDGIGRRIF (2)

SALSRYGGSLFGLTSGHQIVVDLPGVERL

LAQSHHTFNSDPAQYSLCTLVFGSTDTPELRAKFDTSLKDLVPPLERTFLNDAASTANVE

KSRVAERGASLVTFSSNPKEMVRWELSAGIKVLQPETSHSPGKVEANLQSLTRDFGACMS

VPLIYGQDILDRNPRLLDDFWTFDNDLFPLLMVGVPKWAPFRMMQDGLKARSRIIDSLEA

VYRRVDQYEKGEPIDFGADMSDVSMMLRERNKIYNRDGWTFPERAAADLGTFWGLNANAQ

PVLFWFLLYVYSTPGLLKRIRDEISPYIILSDGDKPEIKSMDFPSLFRKCQLFKACIFET

YRLVNEPTSIRCITQPVSLNDGNHKHELQEGTYISVPLALKNKNSSIYEDPDTFIPNRFL

ETDPETGKATARYGKLKPWGLGAAMCKGRTFAEKEIISLGSAVISLWDISPAEGEWKLPA

MIPGTGVKRPVNDIRVVISRRSPFKS*

 

>CYP551A1 N. crassa AABX01000129.1 cont3.541 NCU08716.1 (version3)

67094 MAVLNWASSRAATLSPCPRTAALVVITTWLLYAIGLAVYRLYFH

      PLARFPGRKMAAVTTWYEFYYDFWYGGKYIFEIEKMHKEF (1) 66843

66762 GPIVRINPHELSIHDPEYYNELYVGSSKRRTNFWPLFQGCTDDTD (1) 66628

66552 VNHFMTIDHDLHRQRRKPFDPFFSRMAINIRYWPMLAEKASFLES

      RIRECKGQGKAVRLDRAFSAFSADCIERICTDDLEPGDGFLDQPD

      FGPEWYDGMLGLIRNAPILTKFPKALS (2) 66202

66144 SLRYVPPRLLLWLFPQGRIANKYDARTRTQIHKAVTKQHLSKD

      TTTNSEHTTLFRALAQSDNLSPADKAEERLVREAKLIFLGGTIS

      TGRTLSFVSYYILSRPDIKARLEDELRDVMATWPEVVPTWTELE

      KLPYLQAVIKEALR (2) 65710

65588 LSYGFMRRLPRVSPDVAIQYKEYTIPPGTPVGMSAYLMHSNPEV

      YQDPDQFVPERWLGGDTRVMQRSYVPFTKGSRSCLGQN (2) 65343

65254 LSMAEISLVLAVLFRPDGPRMELFETDESDVKHVHDFVVPLPK

      LDSLGVRVMVR* 65090

 

>CYP551A2  Aspergillus oryzae

          GenEMBL BAE62699.1

          53% to 551A1 43% to 551B1 Fusarium graminearum in CYP53 clan

          Note 551 is not in the 53 clan tree on part C where it belongs

MEDTFNLTVLGGVIVLYSLGLVIYRLYFSPLAKFPGSKLAAATGWYEFYFDYWIGGKYVFEIERMHQVY (1)

GPIIRINPHELSIRDPDFYNEIYVTENKRRSNHYDLFARGIGMD ()

DARVESHIVTVDHNLHRKRRKPLEPFF

SRAGIARLEPALVEMSLKLESRLRQYEGKHTVVTLDHAFSAFSGDIIRRVCFNKDDFGDLFMDHPDFSPDW ()

YNLIHNILRHFPVFTGFPLIA ()

RIVTYIPESILLKAFPQGQSLNRLKDVALQRITHVMNSKATELKDTHR

EVSLFHHIVESDMPESERSPERLVQEAQVLLSAGTVTTARTIAFASFYILARSEIKAKLQAELRDAMDGW

PEKVPTFMDLERLQYLQAIIKESLR ()

LSYGIMHRLPRVSPDLPIQYKDFTIPVGTPVGMSSYFMHTDPTVY

PDPMTFRPERWLGAIDPAMHRNYVPFTRGSRNCLGMNLAMAEISLILAVLYRPNGLKLELYETDETDVMI

AHDFMAPMPKVTSKGVRVLIR

 

>CYP551A2  Aspergillus flavus

99% to CYP551A2  Aspergillus oryzae

AFL2G_08105

MEDTFNLTVLGGVIVLYSLGLVIYRLYFSPLAKFPGSKLAAATGWYEFYFDYWIGGKYVFEIERMHQVY (1)

GPIIRINPHELSIRDPDFYNEIYVTESKRRSNHYDLFARGIGMD ()

DARVESHIVTVDHNLHRKRRKPLEPFFSRAGIARLEPALVEMSLKLESRLRQYEGKHTVITLDHAFSAFSGDIIRRVCFNKDDFGDLFMDHPDFSPDW (2)

YNLIHNILRHFPVFTGFPLIAR (2)

IVTYIPESILLKAFPQGQSLNRLKD (0)

VALQRITHVMNSKATELKDTHREVSL

FHHIVESDMPESERSPERLVQEAQVLLSAGTVTTARTIAFASFYILARSEIKAKLQAELR

DAMDGWPEKVPTFMDLERLQYLQAIIKESLR ()

LSYGIMHRLPRVSPDLPIQYKDFTIPIG (0)

TPVGMSSYFMHTDPTVYPDPTTFRPERWLGAIDPAMHRNYVPFTRGSRNCLGMN (2)

LAMAEISLILAVLYRPNGLKLELYETDETDVMIAHDFMVPMPKVTSKGVRVLIR

 

>CYP551A3P Aspergillus nidulans AN5460.3

About 47% to CYP551A1

VTFYCASLIIYRLYFHPLAGYPGPKLAAATRW

YEFYYDVIRQGKYVYEVNQMHDKY ()

PIVRINPHEIVIKDPEFYNQIYVAGNTRRTEKWXXXXXXXXXXX

GSIVMTVGHELHRLRRKPLDPFFSRMEIDRLEPLIVKEAKLLNHRLQSYSGSGRVLRLADVF

SAFAVDMITHICSEAGPVMMNKPEFGKDW 224

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

ETKNSLFRQLLSPSSA

GGLPESELDAERLASETMVLFGAGTVTVTSTLSLICYYILRDSHIRDRRAGELEGVMAQYPTSMPTWQQLER

LPYLHALVQEGLRLSYGTMRRLPRXX

PDAALHYKQWTIPP  (frameshift)

TPVGMAAYSLHTDPEVYPDPFEFL

XXXXXXXXXXXXXXXXXXXXXXXXXXXXXX

(Missing PERW and heme signature)

LAYAEVYWALAVMFKSNAPKLQLFETEKMDVEHVVDSMVALLRLGSQETRITV

 

>CYP551B1 Fusarium graminearum FG01739.1 AACM01000092 FGcontig1.92_scaffold1

MERLKMDSLNININDWFEKDLPATADWKLFALASVVFVVLRFTCIVIYRI

YFSPLSKFPGPKLAAATHLYESYYDFWKKGQYYKVIQRMHEVYGPLVRVT

PDELSINDPDYYDTVYVNGNVRRTESFGHSFGGGLGIEDTFFASQDHDLH

RKRRKPIEPYFSRNGVLKLENLIGERVEKLFHKFHELSGTGVVARLDYAF

EAFTGDVMQHICIEKPESLLNSDDFSSEWFEMLRNVSLSVPLMGMIPWLV

HVLKFIPESVIMWLAPSAAHFQTFRVQAGRQIEQAKHEKVENDRKGITTV

GGKPTLFRFLVHESGLAPEDLSTERLQKEAMVLLGGGTTTTARTATMTCF

WMLSMPEKGQRLRDELKDIMAEYPKKKPSLTELEKLPYLGAVIQESLRMA

YGSMRRLPRTSPDVALQFKDWVIPPGTPVGMNAYYLHTDPNAFPEPFEYK

PERWLGNVTPAMKRSFVPFSRGSRRCPGSSLALADLHFVLAALFGPTGPK

FELFESDRSDVDAIHDYLMPLPRLDSKGVRVTVK*

 

>CYP551C1 Coccidioides immitis

51% to CYP551A2    Aspergillus oryzae

48% to CYP551B1 Fusarium graminearum

CIMG_09088.2 shortened at N-term

MEAYLPKNLGLRATVAILVISVFYILSLVIYRLFFSPIAKFPGPKLAAVTGWYEL

YYDVVKKGKYLFEIEKMHDKYGPIVRINPHELSIRDPEYYDELYVAGSVRPTERYKGFVDGIVDFDGSHLATIGHDLHRK

RRKPLDPYFSRAGVTKLEPMVAELCEKLIIERFEGFKGTGKVVRLDHAFTAFSGDVINRICIDDPPNLVDDPEFAPWWFE

LFHSGIVSLPLFMGLPWLIHFIRLIPESILAKLDARSQTFNKFKLMCDEHLRVAKREKAALGSKDTTAMGGRLTLFRHMV

SSDLPPSELTDERLSKEAQVLIGSGTITTAGTMGFLCYHIMANPAIRKRLEEELKPVMEGYPQRKPTWAQLEKVAYLQAL

VKEGLRLSYGTMHRRPRVSPKQALRFKEWVIPAGVPVGMSAYFAHRNPHFFPRPLEFIPERWLENVTPEMNRNFIPFSKG

SRHCLGMNLAYCELNHILAALFRPGGAGFSLYETDESDVMPAHDLIVPLPKLDTKGVRVMFH

 

>CYP551C2 Aspergillus clavatus

75% to CYP551C1

ACLA_076840

MEASLHFPIDASLRAAVAVISAGAFYLLNLVIYRLFLSPLAKFPGPKLAAVTSWYELYYDLVHKGKYLFEIEKMHDKYGP

IVRINPFELSIRDSEYYDELYVAGSVRPTDRYEAFVEGIVDFKGSHIATIEHDLHRKRRKPLDPYFSRLGVSRLEPMLGE

LTEKLIVNRFESFKRTGKVVRLDHAFTAYSGDVINRLCMDDPPDVLVDDPEFSPWWYNMFHNGIATLPLFMGLPWLIHVV

RLIPVSILAKLDPGTQTFNKFKMMCDDHLRVAKREKAAQGSKDTSMMDARPTIFRHLLNSDLPPSELTDDLLSKEAQVLI

GTGTITTAGSLCFICYHIVVNPAIKKRLQEDLKLIMANYPAKKPTWAELETATYLQAVIKEGLRLSFGTMHRRTRVSPKQ

PLQFRQWTIPAGVPVGMSAYYAHRDPSVFPRPDEFLPERWLSNVTPEMSRNYVPFSRGSRRCLGMNLAYAEINHVIATLF

RPGGPDFKLYETSEKDVKPAHDLIVPLPSLESKGFRVIFR*

 

>CYP551D1 Mycosphaerella fijiensis

49% to CYP551C1   Coccidioides immitis

e_gw1.2.71.1

MQQHIASSSLLLVVLAITLIYALGVVFYRLFYHPLAKFPGPRIAAATYLYEIAFDYFGHGAYLFEIERMHEKYGPVVRVN

PKELSIKDGEFYDKVYVNGNVRRTEALPSFGDGMDFNHSHGMTVDHHHHRMRRKPLEPFFSRAGVARFEPNLASVVMTLV

HRLRGWEGTGTVLRLDHAFAALAGDIISTMCIESPSMSFLEDRDFAPDWYNLFHTLITSMPIFMNFPWIIKAVRLIPTSI

LKRVDPRSQMFRDWLAEIEKSLRRKANKEVSTYQRGTLKAPTLFDHLVNSDLPSSEMSVERLASEAQVILGAGTVTTAQS

MSHLVVNILLRPEVENRLREELATLMKHLGEARLPEARELEKLPYLQACIKEGLRLSHGLMHRLPRIAPDVALDCNGWII

PPGTPVGMSAYLMHMDDSVYSNPKEFVPERWLGEPDPKMERNYVPFSRGSRRCLAPNLAYAEISMVMAALFSPWSPKIRL

YETNASDVDPVCAFLLPLPRLDSKGVRVIVEESKDNSK*

 

>CYP551-un1 Magnaporthe grisea MG06589.4 53% to 551A1 PERF to heme

probable pseudogene fragment

AACU01000192.1 cont2.1224 continuation of MG06588.4 +1800bp

      FGFMVRLPQCSPDVKLQYGKWKTPRN (0)

      TPVGILAYLMHTD

79064 PTIFAEPYEFRPEGWLEGQVISAMKQAYVPFSKGSRACLGSI (1) 79189

 

>CYP552A1 N. crassa AABX01000480.1 cont3.625 missing about 32 aa

     at N-term (GC boundary at exon 2?)

   2 FFHPLRHIPGPKLAAASYIPEFYWDVLRGGMYQKRILLMHEKY (1) 130

 229 GPVVRISPNEVHCNDWRFIDEIYASGSRKRDKPLHQVRGSGG (2) 354

 419 IVDATFSTTSHDLHRMRRNALNKFFSRAQVARLEPTVRENAERIC

     DKILILGKSAPFDVTTAYSHFTTDVISGYCLGDSLGLIEQKGWEP

     NFREPLYAQLKLMYWLRFIPLFKRLS (0) 766

 888 WYVFFGESFLPCPVSLTNYDIQGDMPRYVRRAQDNVIKGLDDG

     SSVFGALLTSDLPPSEKSLERLTDEGFSLFAAGTETVSWALSVIT

     YHLFANRELLEKVTKEVTQVIDAKSGQLPSWHTLEKLPYLGGVIY

     EGLRLSYGVASRSSRIPVGEDLVYRG (0) 1364

1407 YVIPRGYAIGMSPLSIHHDETVFPNSHSFIPERWIDDNDQHRKEL

     DRALLSFSKGSRSCLGIN (2) 1595

1657 LAYCELYVLLSLLIVRVFPHMKLHETTEVDVTHDHDFFNPFPVSS

     SKGVRAIVV* 1821

 

>CYP552A2 Aspergillus nidulans AN2040.1 53% to 552A1 50% to 552B1

AACD01000032.1  revised 7/18/07

MTISAETVLRLLPLTAGGAWVFFHLAKCIYNLFFHPLSHIPGPWLAAATY

LPEFYHDVVRGGRYTTQIQQMHEKYGPIVRINPHELHCNDHRFINEIYAG

GTRKRDKPVHQVTGSEAAAHATFSTTDHDVHRMRRNALAKFFSRAQVLRL

EPTVRGLAERLCDKMLRTEKGRPFHVGAAYSQFTADVISGYCLGENLGLM

GQEGWEEQIVDEVATVLRLGCTLRFVPWWKHVLGVVLLIRKQLVGSPKES

SDALIVGMPNHIRKAQADIKIMDGKGNGNGPETIPTTVFTSLLQSDLPPQ

EKTFERMTSEGISLFAAGTATVSWALTVATYHLLTKPAILQRLTAEVSDL

VNSSSCTEKVEWSALERLPYLGAVIQEALRLSYGVASRTARVATGEDLVY

RGEWAHREVQYIIPRGSAIGMSAVLAHHDEAVFPDSHLFLPER

WLDEKNQRLKELDRALLAFSKGSRGCLGIK

LAYCELYLLLALLVVRVFPRMRLYGTTEADVAYDH

DFFNPFPVWESKGVRVVV*

 

>CYP552B1 Magnaporthe grisea AACU02000500.1 Magnaporthe grisea 70-15 revised

MATTLVTAQNLLGLAVLWIVYQVLKALYNVSPFHPLAHIPGPKLAAATYL

PEFYHDVVLFGRYTHEIRDMHRKYGPIVRINPNEMHCNDVNFSDDIYAVG

GRKRDKPQHQINGAVLGKSGFGTRDHDLHRTRRIPLAKFFSRAMITRLEG

DMHELVQKLCDKLLAESGKKEPVDVTVAYSCFTSDAISGYCFGESFGFLE

QQGWYPNFRDPTAATLRIAFLLRFFPWCKYFMVVGHWLVDFLPTDIALLI

QTMQIDMPIRVVKTKTDMDSGIQYKRPTVFGSLLHSELDPSEKEPQRLAD

EAGAVIGAGTETASWALAVITFHLLSKPHLLEKLQAELRQVVDGSNNDNS

PKSLPPRSVLETLPYMNGVIQEGLRLSYGVSGRTARVATHEDLVYRGTFG

76746 GNPVEYVIPRGYAVGMSAAVTHHDENAYPDSYEFIPERWMDGHSKKRDAE

RASLAFSKGSRSCPGKS (2) 76940

LALAEMNLALAALALRVLPHATLYETTYDDVLYDHDMFIPRTRKESKGVRVKIN*

 

>CYP552B2 Magnaporthe grisea MG03828.4  51% to CYP552 AACU01001030 cont2.747

MSTTWLLGQSPFAIIAGTWILYQVLKVLYNLSPLHPLSHIPGPRLAAATY

IPEFYHDAILVGRYTHQIVEMHRKYGPIVRINPNEVHCNDVNFSDEIYAV

GGRKRDKSIHQINGAVFGRSGFGTIDHDVHRMRRAPLGKFFSRNNIAKLE

SEIHNLVQKLCDKFLKDSGNVIEIATAFSCFTSDAISGYCFGESFGFLEQ

DGWYPNFRDPALSTVKPIFFFRFFPWTKSFLILVPWIINYLPTDVALLIK

TMQIEMPGRVQKTKAEIESGMSLQYKRPTVFGSLLESDLPEEEKSGARLT

DEAGAVVGAGTETTSWSLSVMTYHLLAKPEVLQRLQDELRQVVDDPKNLP

PWSVLEALPYLGAVIQEGLRLSYGVSSRTARVATEEDLVYRGEFRGRPLE

YVIPRGTAVGMSAAITHHDERVYPNSHEFIPERWLKDKDDPQRKEAERAL

LAFSKGSRGCPGKTLGLCELTLALSAVVLRVLPHARLYETTIRDVAYDHD

TQVPRTVKESKGIRVVIN*

 

>CYP552B3 Fusarium graminearum FG11038.1 AACM01000458 FGcontig1.458_scaffold8

MDSNYDSVQGAIASLTIRQWVIVTVVSWFVYKIVEALYNISPLHPLSKVP

GPKLAGATYLPEFYHDVILVGRYTHAIQKMHEKYGPIVRINPNETHCSDM

AFSEEIYAVGGRKRDKPFHQVNGSAAGTGNAFGTPDHDVHRSRRGPVAKF

FSRAMIARLEEEVHELAQTLCNKLLAESSGKEKSAPIDVAHAYSCFTSDA

ISSYCFGEAFGLLSRDTWRPTFREATLAVLKPVFVFRFFPFLVGSVKYGK

HFVDYLPADAAMLVRTLQIDIPARVTKTKTDLNSGIVYERPTIFADLLQS

ELDESEKGTDRLVEEAVTIVNAGTETTSWALAVITYFLLSQPETMSKLQA

ELATVVDDPRHLPSWTTLEKLPYLGAVINEGLRLSYGVSSRSARVPTTED

LIYRGEFNKQHTTIVLPRGYAIGMSSAISHHDESVFPDSYSFTPERWIDD

DGTIRKNLERSMLAFSKGSRGCLGKNLALCELYLSLTALVMRVMPHMTLY

NTSERDVRYDHDMFIPVTVKGSTGVRVTIEA*

 

>CYP552B4 Fusarium oxysporum

79% to CYP552B3

FOXG_02774 revised

MADATWSAFQGTLTSMTARDWIVALTSAWLAFKVLQALYNVSPLHPLS KIPGPKLAAATYLPEFYYDVILVGRYTHAIKQ

MHEKYGPIVRINPNELHCADMSFSDEIYAVGGRKRDKPFHQVNGSAAGTGNAFGTPDHDLHRARRAPVAKFFSRAMIARL

EQEVHDLAQTLCNKLLAENKDEKERKPFEIAHAYSCFTSDAISSYCFGEAFGLLSRNQWQPNYREATLAVLKPVFFFRFF

PILVSSVKL GKYLVDYLPADTALLIRTLQIDIP SRVQKTKNDLDS GIVYDRPTIFA

DLLQSELDDSEKKQDRLVEEAVTIVNAGTETTSWALAVITYFLLSQPEILAKLQDELKQVVDDPCHLPSWT

TLEHLPYLGAVINEGLRLSYGVSSRTARVPTTEDLVYRGEFNKKPMSLVLPRGYAIGMSAAIAHHDESVFPDSYAFVPER

WIDGDNKIRKDLERSMIAFSKGSRGCLGKNLALCELYLSLAALVLRVMPYMRLYETTERDIRYDHDMFIPMTESGSKGVR

VIIES*

 

>CYP552B4 Fusarium verticillioides

92% to CYP552B4 Fusarium oxysporum = ortholog

FVEG_01613 revised

MADASWSVIHGALTSLTAQDWTVAFISCWFV

FKVLQALYNVSPLHPLSKIPGPKLAAATYLPEFYYDVILVGRYTHAIKQMHEKYGPIVRINPNE

LHCADMSFSDEIYAVGGRKRDKPFHQVNGSAAGTGNAFGTPDHDIHR

ARRAPVAKFFSRAMIARLEQEVHDLAQKLCNKLLAENKDEKDRKPFEIAHAYSCFTSDAISSYCF

GEAFGLLSRSQWQPNYREATLAVLKPVFFFRFFPI

LVSSVKLGKYFVNILPTDIALLIRTLQIDIPSRVQKTKNELDA

GIVYDRPTIFADLLQSELHDSEKKQDRLVEEAVTIVNAGTETTSWALAVITYFLLSQPETLAKLQ

DELKQVVDDPCHLPSWTTLEQLPYLGAV

INEGLRLSYGVSSRTARVPTAEDLVYRGEFNKKPTTLVLPRGYAIGMSAAIAHHDESVFPDSYTFA

PERWIDEDNKIRKDLEKSMIAFSKGSRGCLGKNL

ALCELYLSLAALVLRVMPCMRLYETTEKDVRYDHDMFVPMTESGSKGVRVIIES*

 

>CYP552C1 Magnaporthe grisea MG06623.4  41% to 552A1, 46% to MG01924.4

AACU01000198 cont2.1232

MDSSMELGGGPGGVAVLTFKSIAWMVGLWMVYKVGVALYNISPFHPLAAF

PGPKIAAASYLYEGYWDFWRVGRYTKVIEQMHKKYGPIVRINPDELHCSD

PLFVHEIYAGPGRVRDRWQHAINTGGSGPIAVTGFSTISHELHRQRRAPL

NRFFSRQQMLKLEGEVLDYAQRTVAKMLRTEAGRGAFEIKEAFNCFTADV

IGQYAFGQTMGFVDQEGWEPSFATMVGSFLRSAYVMRHVAIARWASGFLP

FMADYLGEDIRYVMNMLAVTIPGYIKASLDDPQNGRVFADLMQDDKMPEE

EKSMYRLSGEGFNFLLAGTETTAATLTSLTFHLLDQPKLYARLMKDLEGV

DPDKPKWTDVERRPYVWALVHESLRTMPGVSHRSARIAREEELVYKSQDG

KWSYVVPRGTPIGQTSMISHWDEKLFPDPDRFDPERWLLEDGTPNVALAK

YLIAFGKGSRSCLGENLAYCEVYIMLALVAYKILPRAKLVDTTIEDLTYD

HDLIVLQTKKGSISVKVAIE*

 

>CYP552C2 Fusarium graminearum AACM01000116.1 Gibberella zeae PH-1 revised

MDSKSTWETVDVRGIASFKSVATLVGVWFLYRIFVALYNISPFHPLSRFP

GPKIAAVSYLYEAYYDWWRVGRYGKVIRDMHERYGPIVRINPDELHCSDP

YFTDEIYAGPGRVRDKWQHQLNTGGAGPVSVTGFSTVNHEVHRMRKGALS

KYFSRSQMLKLEGEVRDFSQMTVDKMLRYAGGEPFDVKEAFNCFTADIIS

QYAFGESMGFIAQEGWEPNLATWVKSFFQSAYMMRHNVLARKMAQFLPFL

SDYLGEDIKAVMRQMNVVIPGYIKAALKNPDGGRVFAQVVESKTLPESEK

SLYRLSGEGFNFLLAGTETTAATLTVITYFLLDKPEIYKRLMADLDGLDP

ENLKWTDLEQRPYLWAVIHEALRVMPGVSHRSARIAREEDLVYQSQDGNT

NWVIPRGTPIGMTSMINHWNEDLFPEPDEFLPERWLENGKPNYKLQKFLI

SFGKGSRACIGEN (2)

182057  LAYCEVYIMAALMAFRVIPRARLYETTIEDLTYDHDLIVLQTKKGSISVRIAIS*  182221

 

>CYP552C3 Fusarium graminearum FG08186.1 AACM01000329 FGcontig1.329_scaffold5

MDDFNIIALKRFVSPSALLGLAGLWLGYRVALALYNISPFHPLSKFPGPK

IAAASYVYEAYYDWILQGRYGRRIEKMHEQYGPIVRVSPDELHCSDPYFA

DEIYTGKPGRIRDKWQHHLKIAGAGPASQATGTAGPHELHRKRRAAHARF

FSRGQVLKLEDEVLKFAELFIQKMLRWNGKEAFEVKGAFNCYTADVFSQY

AFGEPMGFIEQEGWEPNFGTWTSSFLKTTYMMRHNALARRLADILPIFAD

YMGEDVKRIMHQMNHVIPAYIKTALANPDGGRVFNEILNSNVLPEEEKTM

FRLSGEGFVFLLAGTETTAAILTVMTFQMLHQPKVYARLMKDLEGIDPNN

LKWAQLEQRPYLWALVQESLRHQPGAAGRSARIAREEDLFYKSQDGKTQF

MIPRGTPVSMTAMINHWDTRLFPDPDSFNPDRWLLPDGKPDYTLQKFLIS

FSKGSRVCVGESLALCEIYIMAALMAFRVIPKSELFETTIEDLTYDHDLV

VLQTKKGHISTRIKIS*

 

>CYP552C4 Fusarium oxysporum

84% to CYP552C2

FOXG_10802, not in the F. verticillioides genome

MDQMAVLPASITAAALFQTIAYLFTSWLAYRILLVVYNISPLHPLYRFPGPKIAAASYLYEAYHDWWRVGRYGREIRRMH

ERYGPIVRINPDELHCSDPYFTDEIYAGPGRIRDKWQHQLNTGGAGPVSVTGFSTVNHEVHRMRKGALSKYFSRQQMLKL

EGEVQEFAQMTIDKMLRYAGGEPFDVKEAFNCFTADIMSQYAFGESMGFIAQDGWEPNLATWVKSFFQSAYMMRHNVFAR

TMAQLLPFLSDYLGEDIKAVMRQMNVVIPGYIAAALKNPEGGRVFADVMESKSLPESEKSMYRLSGEGFNFLLAGTETTA

AILTVITYYLLAQPLTYRRLMADLEGIDPQTLKWTELEQRPYLWGVIHEALRVMPGVSHRSARIAREEDLVYKSKDGNVE

WVIPRGTPIGMTSMINHWDKEIFPNPDNFTPERWLEGGRPNFKLQKLLIAFGKGSRACIGENLAYCEVYVMAALLAFRVI

PRARLHETTIEDITYDHDLIVVQTKKGSISVRIAIS*

 

>CYP552C5 Fusarium oxysporum

92% to CYP552C3, 65% to 552C4

FOXG_12228 revised, not in the F. verticillioides genome

MDNFNSIALERLLSPSALFGLTGLWLGYRVALALYNVSSLHPLSKFPGPKIAAASYVYEAYYDWILQGRYGRRIEKMHEQY (1)

GPIVRVSPDE

LHCSDPFFADEIYTGKPGRIRDKWQHHLKIAGAGPVSQATGTAGPHELHRKRRAAHARFFSRGQVLKLEDEVYNYAKLAI

EKMLRWTGKEAFEIKGAFNCYTADVFSQYAFGEPMGFIEQEGWEPNFGTWTSSFLTTTYMMRHNGLARRLADILPMFADY

MGEDVKRIMHQMNHVIPAYIKTALANPDGGRVFNEILNSNVLPEEEKSMYRLSGEGFVFLVAGTETTAAILTVMTFHLLH

QPKIYARLMKDLEGIDPNNLKWAQLEQRPYLWALVQESLRHQPGAAARSARIAREEELFYKSQDGKTQFVIPRGTPVSMT

AMINHWDTRLFPDPDAFNPERWLLPDGKPDYTLQKFLISFSKGSRVCVGESLALCEIYIMAALMAFRIIPKAELFDTTIE

DLTYDHDLVVLQTKKGHISTRIKIA

 

>CYP552C6 Aspergillus terreus

80% to CYP552C4 Fusarium oxysporum

ATEG_03758.1

MNFMIDMNVGLKAALTVFYLIALWAGYRVL

IALYNISPLHPLSRFPGPKIAAASYLYEAYFDWWCVGRYGHEIRRMHERY

GPIVRINPDELHCSDPSFTDEIYAGPGRIRDKWQHQLNTGGAGPVSVTGFSTVSHEVHRMRKGA

MARFFSRQQTLKLEGEVHEFAQLTVNKMLGFAGKAPFDVKEAFNCFTADVISQYAFGEPMGFIAQDAWEPNFATWVKSFF

RSAYMMRHNALARKMAQLLPMLADYMGEDVRAVMRQMNVIIPGYIEQALREPGNGRVFAEVVQSDLLPESEKSLYRLSGE

GFNFLLAGTETTAATLTVITYHLLAQPSIYARLMDDLQGLDPASLKWTELEKRSYLWAVIHESLRMMPGVSHRSARIARE

EDLVYKSRDGRVQWIIPRGTPIGMTSMINHWDEEFFPSPDRFLPERWLVDGQPNYKLQKTLIAFGKGSRSCIGENLAYCE

LYIMAALMALRVVPRARLHETTVEDISSYDHDLIVVQTKKGSISVRIAIS*

 

>CYP552D1 Magnaporthe grisea MG01924.4  poor match 46% to MG06623.4 AACU01000373 cont2.362

MGGILVHVGQAVPTATMAAAYIAGALILYQSAVWAYQISPFHPLAHIPGP

KIARVGRLYEAYWDWWRGGRYMNKIAEMHAQYGPIVRIGHDEVHCSDPNF

SSTLFGSPNQVRDKPQHMCNILVSGALPHSTIVTTSQALHRARRAPMSRF

FSRAHVFRFEDEVLRLASVVADKMLLPERRGKSFDVRDAFNCFTADVVFL

HALGKPLGFVEMQEAWTPNLATWTEMMTDMVYLIKHNAWARWAAIVTPKL

AGMFSTSVKALNELMNVTIPGLLKSATDDVENDRMFPILLRGGMDGKSQV

APGAEMDMFRLGGEATVLLTAGTETTSAALAIITYHILARPEVKAALAAD

LAGLDPTALKWTELERRPYLWALIHESLRHSPPIVARSSRIAPLEDMVYI

PRARDKSADPAPPVTIPRGFTVAMSTITSHYDPTIFQDPAEFRPERWLVK

DGDGNVIGNNEELKKSLLTFGRGSRACIGENLAMCELFIMTAMMALCVIP

RARLDDRMTADYVTYDHEKLTLQTIYGNVATHMVVS*

 

>CYP552E1 Aspergillus nidulans AACD01000108.1 Aspergillus nidulans FGSC A4 revised

MDFLMEYGSPAGFFTIFATL

WLVYCLLRMLYNVSPLHPLSHIPGPNLAAATFLYESWFDLVLGGKYTHKIKRMHEQY (1)

GPVVRINPEELHFNDISFANEIYAGPGRKRNKQVHYLNIFAGPTTSS

MLATVEHGHHRVRRSAVNRFFSRAQISKLESNIKDLALGLCDKMIRL (1)

AYSCFSGDVISKYCFGEPFGFIAQEEWEPNYRNALKATQAPVHVFR

TFPFLKRLTDIAPLCARWASPGIREMLIESNEKMPARIRKARQDSKRGIT

ETQPSLFAALLDAPLPEAEKTDQRLGGEGFLMIAAGTETTAWTLTVITFH

LLNQPDTLNRLRCELQDADALNLQWFALERLPYLTAVIHEGLRLSYGVSQ

RISRIAPTETLVYQGEHNGRRIEYSIPSGTTMGMSNAINHHNEDAFPDSD

AFIPERWINIDDAQRRRMDSCLTPFSKGSRQCLGINLAYCNLYLALTALT

LRVLPWMSLYETTVDDVRYHSDLFAPLPKKGTKGVRAIISYP*

 

>CYP553A1 N. crassa AABX01000379.1 cont3.619 NCU09565.1 (version3)

26616 MPSTMADAVAEIESLLDTGIAAIKGLIP

      MIWVQSKTSPVQFLVGGVAVLLSYLLLTISPPHDPSEPPLIKPR

      FPVIGHLFGLLNHQVNYFSELFARYHLPIATLPIGNQKLYVIFD

      APLQQAALKAKDMDAQSFMVDFVPRIFGVKQGTVDKLLGKDGVH

      PNIMGDMEQVFKSALSGDNLQKLASTTLATMADTLNDVDSKKGT

      KIPNMFLWLQSLLSRSTSKALWGEKHNPFKDHQVIDAQW (2) 25888

25827 DFESHLGPLVLGVLPSIVARRAYLARNKVQSALLSYFSARHYET

      DPSTSYFIRARTRLLKRYDLPDDELAKNEVAITLVATTNVLSTL

      FWCIAEIWTRPDLLQQIRAEALLAVFGNSQKALSGEDMGTRTIT

      IPATGLDAKCPLLASCFRESIRLASQIVTARRVLKDTLLTDPVS

      GNSYLLKADTNVMMPAKVVHRNTSVWGADAEGFNPRRFMWEELG

      PKVERQRKAAFVPFGGGKHMCPGRHFAFTENLALMAALAIGFEI

      EGLDRSKLKMGDSKRGETAKPLPGLEGGEVVLKKRKGWEGVEWEFSC* 24892

 

>CYP557A1 AY316198 Rhodotorula sp.

MLALVCVGLFLYLLCKYRSHAIATSDRTDLAGPKGWPLMGVFKF

PLPTLSVTYVVVDVTEKTLSGNLIFYATQSSTRGLEWGVRFVKTYGHAWTLSVPGLRL

IDISTRPDWIEYVQKTNYHNYVKGSAFRNRLSDIIGNGIFVTDGRVWQFQRKVTSHIF

TGRSFQDAICPAIAEELRSLNKLLDNYADTGETVDLQDVFYRFTFQAFGRFAFNLDLR

NLEIDHKPVPFTTAFDQCQKPSLDASSTRSGAFASALPLPAVEYQTPPSCFRLCLRND

DKRAALLAKKDAKVPSDLLTMFMTARYDDSRPLSRVEIRDAIINLLLAGRDTTAQSLT

WALYRLVDNPAHQRLVREEICGQLAGGTEPVTLENVKNLVQTQAAHLESLRLHPPVPR

IVKQAVKDDALPNGGPLIKAGEFIRLSDWALGRNEEVWGADAKEWKPSRWIDDEGRPI

QYSQWKAHFFNGGPRICLGKSLATLEGVAVIANLLHRYNIAFAPGWWENVEKTGRIAG

DLEETPLYGPALTLPMKSPFWVTISRR

 

>CYP558A1    # Achlya ambisexualis (not a fungi, an oomycete Stramenopile)

             No sequence confidential

 

>CYP559A1 N. crassa AABX01000439.1 cont3.339 NCU05967.1 (version3)

53814 MAITSLSSHLLESLLASLTLGRVTALVILFTIASFAIDSTRKPTYPKSLP

RVGFTGGIGATVRNWMNLGNYHNWVEEGYNKYGKHGRSFVTPSSPSQPDE

IVVPQEQIAWMLEQPETVASTQESHKDVLFGTYNFFNVDDHFPTKVMHKH

LARNLVTLLPDIQQEVAESIDIAFGTDTENWTTLNLWEALLAIVPRVMNR

LAVGLPLCRNQAFLSSQVGFADDVFRNCLILTLFPTVLHPLVGRLVTLPN

WYHFHQGYRLLKPVIEQRLRDYERFESGESQTPPEESLITWLIRQAKADN

LPHEQTPYMIAMRLLPIEFAAIHTTVITLHALFLDLLASDPSKDYLSILR

EETTAVLQSEPNGIWTKNGLAQLYRTDSAIRESMRLSTFSTGLTHRKVVA

KEGITNPFEGWHAPYGAILMANMSVQHDADIYEDPKTYDALRFSRVREEY

EARSQEEVEKGKETEEALRIKRLGVVTTSSEFLPFSHGRHAC (2) 55289

55352 PGRFFVAHEVKMMLAHILQNYDIKPIAERPRPTWVAHTIIPALD

VKIEVKRRQRKK* 55532

 

>CYP559B1 Magnaporthe grisea MG00572.4  50% to 559A1 AACU01001430 cont2.99

MAGENLPLEILDRISVGRVLAGIFLFVVTSFIVDILQQPSYPKSLPRVGY

GSGPISTVRNWLGYVFKFPQWVDSGYQKYSKEGKAFVVPSAASRPQEIVV

PRTQTAWMLDLPERQLSSAHAQSDVLYSEYQFLGSEFATDSFHINIIHRN

LTRHLSTLIPSLQDEVQVAVDETFGTDTENWKEFNLWESWVKLVPSVINR

VLVGPELCRNKELLNSMVHFADTVVMNSFILSLFPKTLQPIIGRALAVQN

WRLYKKAHKILEPVINERLEMMARKDAGNHAKMADWEPSEDFITWAIRMA

RNEGRSGELNSVNITKRLLPIEFAAIHTTVITGHFLLLDLLTSDPKKGFM

ESIRQEASSMLQRSGGRWTKDGLWNLYRTDSAIRESQRLSIFATSLTKRK

VVAPEGITNPVEGWHVPQGGYLMLNLDGPQHDEDLYENPKEYDAFRFSRP

REEYEARPADKKDLEEYMQLKKLGMVTTGDAHLAFGHGRHACPGRFFVAH

MLKMTVAHLLCNYELEPLPEKPKAAWMGQLIIPPVDVKIRLRRRKQTA*

 

>CYP559C1 Nectria haematococca

fgenesh1_pg.scaffold_20000187

Necha1/scaffold_20:493296-494983

47% to 559B1 Magnaporthe grisea

MEIDISRLFLYAVVFLVVSFIIDALCQPKYPTEIPVLGSKGWFSKIRNSFAYFTKHQSWIDQGYQK (0)

YGKNGLAFIAPAPISRPPDVILPRSQIPWMMDQPDHILSAVKAHDQILYTEYNFLGNHLSHEPFASRVVHKYLA

RHLPSLIKPIEEEVNAAVEDSLKLKQDGYTKVNLWELWMAIVPRVTNRLLVGEETCRDDVFLQSMIRFTD

DVVRNSFLLHAFPKILHPIVGRLLTIPNYLHWRAASRRVLPIIQQRLENMRRKDAGELKDWTPPEDFITW

DIRLAMAEGKPFELDPVVISKRLLPINFAAIHTTVLTGHSWMLDMLSSPPSENILETLLSEINTHKPTTG

WTKQSLTSLLRVDSSIRESQRLSNFAANLIERQVVSPTGLHNPEYGWTLPKGAFVTVNLQGTHHDEDIYE

GAMSYDPWRYSRVREAWEGKSEEERKDEDGVKVRGLGMVTTSDAHLAFGHGRHAC (2)

PGRFFVAHELKLIMAAFLLNYDIKMIDQRPKPQWMGATIIPPLDACIEIRRKSKS*

 

>CYP559C2 Fusarium oxysporum

71% to CYP559C1 Nectria haematococca

FOXG_06404

MTVLGDQLTEVTFTRVLLFGSLFLLVSFIVDLLTQPRYPSEIPVLGHSSEKWFSRIRNSLAYFTQHQAWIGEGYEK (0)

YGKNGLPFIAPAPISRPPDVILPRSQIAWMMEQPDRILSASHAHDKILYTEYNFLGKDLAAEPFANRVMHKYLARNLSSAIPAV

EDEVNGAVEHALETMMKAAAEKDPKNVDGEGYTKVNLWDLWLAIVPRVTNRLLVGSPACRNPDILKSM

VSFTDNVVMNSF

LLHGFPQVLHPIIGRLITIPNYLHWRKASHVLLPIIEQ

RLKHMQRQEAGDPEMKNYTPPEDFITWDIRLAMAEKKPFELD

PVVISKRLLPINFAAIHTTVLTGQSWMLDFLSSPPSVLDALRNEILEHKPSEGHWTKQALSSLVRVDSSIRESQRVSNFA

ANLIERQVIAPEGLHHPDYGWTLPRGAFVTVNLQGTHHDDEIYEDAMSYDPWRYSRVREEWEAKSAEEKQEKEEEAKKIR

GLGMVTTSDAHLAFGHGRHAC (2)

PGRFFVAHELKLIMAAFLLNYDIKTLEKRPKPQWMGATIIPPLDACIEIRKESTT*

 

>CYP559C2 Fusarium verticillioides

95% to CYP559C2 Fusarium oxysporum ortholog

FVEG_04255

MTVLGDIFTEVTFTRVLLFGSLFLLVSFIVDLLTQPRYPSEIPVLGHSSE

KWFSRIRNSLAYFTQHQAWIGEGYEK (0)

YGKNGLPFIAPAPISRPPDVILPRSQIAWM

MDQPDRILSASHAHDKILYTEYNFLGKDLAAEPFANRVMHKYLARNLSSAIPAVEDEVNGAVEHALETMMKAAAEKDAKN

VDSEGYTKVNLWDLWLAIVPRVTNRLLVGNPACQNPDILKPM

VSFTDNVVMNSFLLHGFPQVLHPIIGRLITIPNYLHWRKASRVLLPIIEQ

RLKDMQRQEAGDPEMKNYTPPEDFITWDIRLAMAEKKP

FELNPIVISKRLLPINFAAIHTTVLTGQSWMLDFLSSPPSVLETLRDEILEHKPSVGHWTKQALSSLVRVDSSIRESQRL

SNFAANLIERQVIAPEGLHHPDYGWTLPRGAFVTVNLQGTHHDDEIYEDAMSYDPWRYSRVREEWEAKSAEEKQEREDEA

KKIRGLGMVTTSDAHLAFGHGRC (2)

GRFFVAHELKLIMAAFLLNYDIKALEKRPKPQWMGATIIPPLDACIEIRRKKST*

 

>CYP559D1 Mgr051 Mycosphaerella graminicola

42% to CYP559B1   Magnaporthe grisea, 41% to 559A1, 39% to 559C1

MELLTLVLQHPFLAIAAVLVGLYAYALVTGSSKPAVPGELPWVGKKDGQWFSLTRATWMSVGKARQWLHEGYTKFGRNGKSYLFPDVSGRHEIVLPPDQLRWLTNLPDSTTCVRAAHYDILQGDYAFTDKYLLESFHERVIHKNLARKVDATIPAMWDEIDASFRDIWGTDTTQFKDIPLWDSVMAVIARLSNRLFVGLPLCRDKDFLAVSGSFAMDVITLVSLLPLFPKFLQPILGRLGSLPGHYHYWRTRKHTLPIIRQRLADITALAADPTTKLDRPIPEDYITWHIRTAMADNRPKDLDPVMISRFLLPIEFAAIHTTALTLTNILFDLYSSDPSQNYIASLREEATRLHAECNGNWTKQILTRMLRTDSAIRESMRISNFATRAVLRKVISPAGLTHPTENWHTPEGSYVSVDQHSVHHDPAVYPSPEIYDAFRFSRPREEFEAKHAGSDAKTLAQDPEYLAMRNLSMTTTGENFLAFGHGRHACPGRFFVQHELKMVVAYVVMNYEVEVLPERPKSMWLGTSITPDAKKALRVRRREGTV

 

>CYP559E1 Mycosphaerella fijiensis

45% to CYP559D1  Mycosphaerella graminicola

estExt_Genewise1.C_70615

MELITDHPVVAIAFVVWILTALGSLTSRSRTKLPDIPWMGKSGNWPGAEYLATWSALASMRRWYSEGYKKYGK QGQAYIL

PDVLGGKHVVLPNEKLGWILEQPDSVLSTGAAHYDALAGDYAFTDSQILKDLYHEHVVHQNMARKVGEVIPRTWQGICDA

FDELWGKDTQQWKTINIMDSMTHLVASVSNYFFVGEPLCRDKQFLRAGRAFADAVISCLFFNQLLPNIAKPFLMPIIAIP

NHWHHWKIARAVLPFFRKRMIEMEEAESKPGSTIEIPEDYITWHIRTAKKEGRLDMLQPYKVTRSLMAIEFAANHTTLLT

ISAALIDLFGSDPKLGYVEGLREEAERVYREHDGVWSKQAVNKLVRADSALRESMRLLNITYAVTRKVLAPEGVRTPDGM

LVPYGQYISVAAEERHYDADVYENPHVYDAFRFSRSREAYEAANPEDKNSQEYLKHQSKSFVTTSQDFLPFGHGRHACPG

RFFIQQELKMLFAYMLMNYDVQHLPERPPNFVWGPIHTPPPKTTLQVRRKERTL*

 

>CYP559F1 Mycosphaerella fijiensis

45% to CYP559D1 Mycosphaerella graminicola

41% to CYP559E1   Mycosphaerella fijiensis

estExt_fgenesh1_pg.C_130046 revised at N-term

MAGSTIENVVDQLDGASWWLIAALCLVCYTTYSAFTSNAIVPRDLPW (2)

VGRKGRGPFSEIRAAIQCLLHQREFLAEGYHR (0)

YLSKGKSYVFPTLNGVSEVIIPSNKLPWLLDHPDSKASNVEAHF

DMLAGQYAFTDPKLLHHPFHEEVIHRNLGRKLANLIPPTWDEVKFQVDNTLGRNTEQWKEIHVLEAMTDIAAAISNRMIV

GLPLCRNAKFRDLQARFAAGVMFVVMLVQLSPKLMQPIVGHLASIPNRLVVWQCKKMTLPVIEKRLADIEHEGKNSEEKG

HTDIPDDYLTWQIRLAKERGDAKEYDADWITRRLMPLEFAAIHTSSFTIANILLDLLGSDPKLGYIEAIREEAETVYREA

NGQWTKQDLARMVKTDSAIRESMRVSGFGVRGVQRKVIDKNGLKDEEGWTAPYGSLIATDVWTRHHDPAIYPDPYRYDAF

RFSRAREQ

EDYLKSQSLSLISTSDRFLAFGHGRHACPGRFFINLEMKLLLAYLVMNYDFQYLQKRPENTITGEVIMPPMK

AGISFRRRPNSLFAAL*

 

>CYP560A1 N. crassa AABX01000445.1 cont3.580 NCU09274.1 32% to CYP503

22936 MNTLHNISSPPGPLDPYANPFHLAIKDRIEHLSYPKTFALILITSWFLST

TAKWLTNGKRKKLAIPTWGYRSWFEPTLLLQARFVMGARQLVHEAYTK (0) 23229

23290 MPNMPFVLKRYDADFLILPIRYLDELRLIPLSKLSNKGANVA (0) 23415

23484 NLVPEYHGIT

FLLTNNLHVEVLRKKLTPELWKFIDLASTELSHGWDISLPHSQLSANGND

WVKIDFERTVRDLVSRMTAKVFLGNPACRDETWLGISVAFSIDLFTASFT

MKMFPPWMYPVVAPLIPARRRVLKQLKAARGIVGEQMRKHASLKEKRAQA

ARGEVSLTAEEAQDTLLNWMLDHGKGDEVRVEEMSARQCVLTLASIHTTA

SQAANMMFDLCEHPQWWGVLQEEVDEIAKELGPPGLAGGMKEGGGGVSVK

EWCVRLEKLDSFFQETQRREPPIM (1) 24335

24430 LGPQRLAQQDVTLKDGTFIPRGTRVA

FPMLEHQMDPQTYENPNEYDPMRFYRKRHESPDQMHLHMAGQTHPNVLGF

GYGNQACPGRQFAVAEIKLIMARMLYEFEFKFADEKKGRPKTGYINEMAM

TDWNARLLMRKRVR* 24852

 

>CYP561A1 Magnaporthe grisea MG00301.4  39% to 65B1 AACU01001402 cont2.70

MSIPQALGLGVLLVLGYLASVAIYRLYFHPLAKLPGPRLYAITSLPFLVR

DKILGTWVMSTTELHAKYGRMVRFAPDRVSVDGSIGWSDIYQRRPNLPEF

DKDIRTYQVGRLGLLPALRDDHRRQRRTVARAFSVTALKEQETYVQTHLD

FFIGRMHEFAAKDADVDISNWLNYLTFDIVGDLALGEPFGCMANGGYHPF

VSMIFDTAKANAMGNFMDNFKFLSPLVKLMGKKELRRNMAIIEMAAATTE

KRIALGPDARKDFMTYLLRKSKDGEGLTHPEILTNSRVLIVAGSETTATA

LCGLIFYLSRTPQAYRRLVEEIRSFTSEDQITMQTLEGLPYLNACLEEIL

RIYPPAADTPPRISPGAEVGGYYIPEGTVVSIYQWATHHNPDNFVNPLTF

APERWLPRSNPFYESIYDKDNKAAFRPFAVGPRDCVGKNLAYSEMRLVIS

RLLWNFDVTLVPGQDDWMKRQKVFLLIEKDDLFVKLKPVKRS*

 

>CYP561B1 Magnaporthe grisea MG09637.4 probable polyketide synthase AACU01001669 cont2.1850

MLKLSYLGVILAVAYISYLLGLAIYRLWFHPLAKFPGPAWLAVSEFPYL

FMGSYRGTFAARTLDLHRRYGKIVRIAPDRLIVDGSVGWNDVFAHRPGGD

ATEFHKAKGTYASPDGTALINATSRESHRRQRRALAHAFSEAALAEQEQL

IIRYVDLCMRRLSEASRGGSQPVDMMSWLNFLTFDIIGDLAFGESFGSLE

KGGYHFWVKNIFAAFKGTSRRRFFRRTGLSLLARLDPEKTVEKLTANNRY

SHEK

AWNGELDAQGRPVMKSRRDFMSYMMRKSGTDQ

GMTREEILNNTVTLILAGSETTGTNLSTFFFQLCLPQNRAIKDAVVAEVR

SKFTGEADVNFRSVQGNALPLLHACLEESLRIHPPVAETPPRVSPGAVVD

GRFVPRGTIVMVYQNATYRNPDNFVEPDAFRPQRFLPPSHPMYDARLAAG

SNTAAFKPFSYGPRDCLGKNLAYAEMNLVASRVLLRFDVELAENTPKDWL

DSQLSFGVWQKDDLMIKLGERRDVELKP*

 

>CYP561C1 Magnaporthe grisea MG00059.4 37% to several families AACU01001358 cont2.16

MAASAFSPENILLGFAGKSTMGRVTYAMQLVGIGVAIYWIILATYRLYFH

PLAKFPGPRIRAITGMPYLYQSYVEGTFARKVRALHAEYGNVVRVAPNRL

AVDGVVGWKDVHAVRAPSPEMPKLPGYYGPDSHRSLVASPTREGHQRHRR

ALAYGFSNTAMHEQEPVITYYVDMLIRQLSKRSKEGPLDMAMWFNFLSFD

VVGDLAFADSFNILKKGKYHPWTSNLIKGIEGATLNRFFLFSSPLLAPLA

LLDLNGTIKTFWENRRIADSKTKARIDLGPDPPVQTDLIGRDGKPVVRKD

FVGYMLRFNQKASNEALSEVEMKRNANIIMNAGSETTATALAVLTFMFSL

PENRIWMERATEEVRATFKSEDEINLHSVSNHPLPILRACSEEALRFHPP

APDMPPRLCPGGTVGDYYVPKGAIIQISQLATYHNPDHFLECDDFRPQRF

LPPDHPLYEKRFANDNFSVFKPFSYGNRDCMGKNLAYIEMRFVMCRILFR

FDYLGLDYKGGATDQWLNEHRAFIVWKKPPLMVIFREREGLVPLEPPEDT

DD*

 

>CYP561C2 Magnaporthe grisea MG03825.4 poor match 53% to MG00059.4 AACU01001030 cont2.747

MGSIAKEPLSSLDLLAAKGVLERLPTLGNWLLVGTIVYYVGLAIYRLHFH

PLAKFPGPTLSAVSGIPWLWESYITGTFTWNVRKLHEKYGKIVRISPNRL

AVDGSVGWSDIYSRSGGSEFSKYPGFFGPNGDLTIVGAMTKESHRRYRRA

LAHAFSETVMHEQEPLITYYVDLFIRRLSDASRDGASFNMVNWLNYVTFD

IVGDLSFAHSFNSLENRKGHPWIENMTTGILGGAYNRFLVEFPLAFPAVF

FHIRKAVNAFWTNRAYADEKSRARIAKGSADEVTDMVGSDGKPVVRKDFI

GYMLRENKDKEALTEVEIVRNAETLMNAGSETTATCLAALTYLLSLPENR

DWLNHVTNEVRNYFKREADVTLRSVNPKALPILNACIEESLRYHPPASET

TPRVSPGALVNGHYIAKGCVVQIYQLATHHNPENFLECDSFRPQRFLPPT

HPLYERRFANDNMASFRPFAYGSRDCIGKNLAYSEMRLVMARTLLRFDFV

GVDPVSKDFLTSQRAFTVWVKPSLWVKFKERTDLELKA*

 

>CYP561C3 Magnaporthe grisea MG02329.4  poor match 54% to MG00059.4 AACU01000455 cont2.461

MGISTPENVVPLTGTVTKGLLERLSGLGSVLAGGLIVYYIGLAVYRLYFH

PLAKFPGPPLHAISSMPFNIQSYLQGIFTWKVQGLHAKYGKIVRIGPNRL

AVEGSIGWTDVYGHRPGGTETEFAKLQGFFGPVPDHGIIAAPNREEHRRH

RRALAHAFSDTAMHEQEPLITYYVDLLIRRISEETRTGVPSDMLKWFNYV

SFDIIGDLSLAESFNALQTAKGHPWITNMFKGIKGGNYNRFLRLTFPMLL

PIAVFFDPTGAIKAFWENRLYSETKCKARLAKGADEDQTDMIGSDGKPVV

RRDFIGYMMRENRDKEKLTEQEIIRNSNILISAGSETTATCMGALSYLFT

LPENRQWRDAAVNEVRTHFKKEADIQLNTVGANVLPILHACIEEALRIHP

PASETPPRVSPGAFVGGEYVPKDTIVQVFQLATYHNPDNFVEADKFRPQR

FLPPSHPLYEPRFAKDNMAAFKPFSNGPRDCIGKNLAYTEMRLIMSRVFL

RFDFESLLPESENWLTRQRAFTIWEKAPLMIKLKERTDLEVKA*

 

>CYP561D1 Fusarium graminearum FG01740.1 AACM01000092 FGcontig1.92_scaffold1

MELPSFSLKFDHEKLLPLAIISTGLLVTSLVAISIYRIWFHPLAIFPGPK

WLAISNVPERYMSNISGTWIWRVSSLHRKYGPIVRIGPNRLAVDGSIGWF

QVYAMRGKEDEFPKYPEYIFPGDGLSILGANQVNHRRHRRQFWSAFNDQA

LVEQEIVIQPYTDMLLQRLSEQAKIGKPINIVDWINFLLFDIAGELVFSS

PFDCLDKQEYHPWVANFFRAVKGNAVNRFVTHYPITKPIVNFLFTGKEQI

QREADQRNMTFHHAMQRMKLGEQPTPGRRDFMSFLMRRNRDGGGLSDTEI

LVDCPVLIGASSETTTTALSGFFFYLGISPQAYKRLVEEVRSSFRSESEI

NMKTTKQLEYLNATVDEALRVYPPAAESPPRISPGAEIDGKYLPKGVVVS

VYQWGTFHNPDNFADPDEFIPERWLQPSHPLHNPKYDNDNRSVYRPFGFG

MRDCLGKNLAHAEIRVVVSRILYRFDYELAPNQENWHANQKCFMAWDKTP

LILTLKPRDFAP*

 

>CYP561D2P Fusarium oxysporum

pseudogene 51% to CYP561D1 Fusarium graminearum, & = frameshift

FOXG_13060

LIVAIPAVIIYRLYFHPLAEVPGRKIHAITGFLTQWKSHILGTWL*EAAQLHRQY

GPIVRIGPNHIAVDGSIGWPQVHGHQPGKAEFSTYPNFIFPGDGMSLIGAQKDDHRRQRR

*LAHAFSDAALVEQQSVTLQYIDLLIKQLTQRAESGEPIDIVTWVNLTTFDIIGDLTFSE

SFGGLKSGGYHPWVQNFFDNIRGEGMMRLMDRYLLLKPLILAIGYRHI*ESMESAEMSMG

KAKARVALGEQPFEGRRDFVTYMLRRGKDGVTAMSETELLVNSSIVIGAGSETTATALSG

AFFYIGTHPQVYCYLVDEIRGAFTDASDITLKSTAQLQYLHACIEETLRIYPPAAETPPR

VCPGATIGGKYIPKG (0)

TVVTVYQWATFRNPSNFADPDSFRPER*LSQ &

THALDDEKYAGDNRAVFKPFSYGARDCIGRNLAYAELRVILSHILFQ (0)

FDFELKPGQSDWHEKQTSFLVWDKDPLNITLKLRQDEASIS*

 

>CYP561E1 Fusarium oxysporum

39% to CYP561C3

FOXG_17449

MLLLDFIIMFRPLFDSWPLSALWL (0)

LIIYRVTIIVYRLCFHPLAKFPGPKYLSVSSLPFRYCSHIQGLFFKTVEQLHAKHGPVVRIAPDSL

SIDGLYAWESIYTHKSSGLPEWSKVPGHFFP (2)

GDHNVLINAPRETHRRQRRAIAPAFSNVALNDMETDILRHIQELLRALASKASNTQENSAESIDIMS

WLYLLTMDTVSDLIFSNSFNGLKDRMQQKFLLGFFDSTQGLQIGSFLMAYPLLMPFLPLI

YMADVGGLKSSHKYGSFIEAKAKTRMTTGLHSGARRDFIVYMTQDREDAAMETSEKKGMN

DLEISQNSLILATAGSDSTTTTMCGVLYYLAQPAAAKAKRAVLDELRSAFQSEEEVTLRN

TTSTSLPYLNACIEEAMRVYPSTAEQPPRVSPGELVGGHFIPKG (0)

TKVYTYQWSTHRNPAHFAQAKEFHPERFLPESHQQYDKRFLDDRLSLVKPF

SHGPRDCVGRNLAYALMRLTLVNFLLRFDYELAEPKEDWLANQTVKFSWTKGPLRIRVRE

RNEAR*

 

>CYP562A1 Magnaporthe grisea MG05390.4  poor match 41% to MG10954.4

AACU01000502 cont2.991

MSGRTTDRRACARRRVIIPPASGAGVCRGHVDQSSAPWHPHVNHRVRVSV

LYKACPARPSPIFVSGHSDSLYTDGTCLVYAFYRVVYNVFFHPLRQYPGP

WHRGASRLPYTISIFRGDVTFRVKALHDKYGKVVRITPDTLSYTDSQAWS

DVYSLKKTGSSGNLPKDPKFYVAAPDTSTANDADHRRLRRVQAHAFSEKA

LTMQESFIQQHTDRYIKCLERAAQEADGVVDVVKWVNFLTTDLIGDLSFG

ETFGGLASGRLHPWLESVFTTLKTFTFIREFLRLPSWATRLAVACIPKHM

LEHQRSAVAFGADAARARIERGSDKPDFMHYILRHEKDDEKGRDLHGRHH

IHRRRERDYSPVAATMISGTVYLLLQHPDVLHRLVSVIRTDFPAIDDMNL

VNLQQHEYLNAVIKEGLRLYPPAADFLFRTSGGQSVVVAGRVVPPKTSLT

MNLWAMYRDPTNFHRPLEFIPDRWLKDAPAEFDKDDRACFKPFSVGPRDC

IGKNLAYAEMRLILAKMAWSLDLLSVEPDSQGWIEKQKIFGLWEKPPLNV

KLAPRVR*

 

>CYP563A1 Magnaporthe grisea MG07593.4  37% to 65A1

AACU01000919 cont2.1406

MSASVSIHSLGKNAPTIIAIAALATIATIAQFLWKRRSHPLRSIPGPWIN

SLSEIPAALALVKGDQHIYYKSLHEKYGRVVRVSPNELSFLSTEARQEIY

GLRKGGLNMEKSPIFLGAVGAVGGETGVSLALNADHTRQRRALGYLFTNS

ALLRFEALMKLQVQKFIAVMEEKTLKKEPVNVANWFTYLAFDIMGDLCFA

EPFGCLDQAANTEWSTSVINVFVSATWTQGIRRLSGTGTWLESLMTWLLV

PAKAAEWRRTHLNNSRERTLRRLASSEQDHADFIYHILNSEGKSTLSHNE

VILNVALMISAGTDTTATALTGWTYFVCTHPEVYQRLIDEVRGGLPTEDH

IRWENVRKMSYLEATIHEALRLYPPSPASQQRVVPRGGATIDGYFVPEGT

TVAVPPWVSTHSEMHFYDPESFRPERWLGEDDKYARDDFNASLPFGTGPR

VCIGRNLAYMEMQLITATLLWNYDLELDREKYLDVNSVWGLDGNMKRMKV

FHSMTKPDLWVKLNKVRQ*

 

>CYP563A2  Botrytis cinerea = Botryotinia fuckeliana

          GenEMBL AJ851088

          join(852..944,995..1157,1224..1300,1351..1597,1658..2661)

          aba2 gene (note: CYP527D1 = aba1 gene)

          57% to 563A1 in the CYP53 clan part A

MLLSIKDLSEKYIMLLDVKDLSTLKTTVAVLVTVALIAQVLWKI

FFHPLSAFPGPWFNRISEIPGSWVIATGKQHSYYRKLHEKYGPVVRVAPNELSFIGDR

AWDDIYGVQKKGPNFEKSPIFIGAVSPLDGQTGISLAPNEAHTRQRRALAHVFSNTAL

LQQEEIMRSHVDKLVGQLKKTIAENRPINFSNWYTYTTFDMMGDLCFAEPFGCLDQGG

ATEWSTSVINVFKSAAWDQSIRRVAGVNTWLQKLMVKLLIPSKAANWRKVHFQNSREK

TLRRLADGNREHKDFIYHILKNKEAKNSLSETEIILNMVLLISAGTETTASLLTGWTY

FICTHPEVYKRLTDEIRGRFNSEQDITWETVKDLPYLHATLSEALRLYSPAPANQQRI

VPPGGSVIDGHFVPGKTTVAVAPWAAINSSLNFKDPQKFIPERWLGDERFVNDKLNAS

QPFSLGPRGCIGKNLSFFEMRLITSRLLWNFDVSLVTTGEHGETNKLWDMDGAGKYMK

VYQTWNKPDMWVMLKEVPR

 

>CYP563B1 old name = CYP65N1 Magnaporthe grisea AACU01000984 cont2.1481 MG07982.4 C-TERM EXON

AACU01000985 cont2.1482 N-TERM PART (3/4) RUNS OFF END

MYGTNLLETMGKPTAGHLGMA (0)

ILVAFTIHVLRMRFFHPLRRYPGPWLNSITQIPAAWALLRARQPKAYRELHEKY (1?)

GPIVRVAPNELSFINVEAWDDIYG

XXXNFEKSPVFIGAVSPLNGQTGISLANX

NEEHTRQRRALAAPFTNRALLQQQDILRVHVDKLITALRAKARNKESVNMGEWCMYL

TYTTFDIIGDICFAEPFGCLDGGESNEWARAIINIFKAATWDQAIRRVAGTGTLLHKALV KIIIPAEAAQWRTIHFSNSKAKTLARLADPDRQHPDLIKHILDSEDSRAALSPTEIILNM VLFISAGSETTANTMTGWTYFMLRHPEARARATAEVRAAFASPRDIKWED

(SEQ GAP HERE)

MGK (0) NLAYFELRLVLAHLLWHFDLEPTDSAAGRECMRRWEQTDMD

TYQTWMKPDLVDVYTQ

 

>CYP563C1 Mgr027 Mycosphaerella graminicola

50% to CYP563A2 Botrytis cinerea, 45% to 563B1 Magnaporthe grisea

MNEGMPSTLTLATASGLIATFFIFILCRTIFHPLRSFPGPWLNATTELPSALRLAKGQQHIYYHRLHQEYGPVLRTAPNELTFTSDQAWNDIYGLKNGVVLEKSPIFIGIASPMNGCVGVGLAKGKEHTRQRRALAPGLSKTALYKQEEIIQRHVSALLDLLRENVERDEPSNIAEYFSFTTFDAIGDLSFSEPFGCLEQNKTTEWASAMRQVVIFASYDQAIRRVAGVDTWLRDLLLKVCMPAKAAHWRMLHVKKSLEKTAQRLEKPDSEKQDMIYHLLREKDPKKSLNHDEIKLNMMLYISAGSETTSITLTAWAYLIGTHPEAYRQLTDEICSSISSSADIKVDNVMSLPYLGATINEAMRLFPPGAVAMQRMVPPGGVTIDGHYVPGGTTVSVAPWAASRSPDNFHEPDNFRPERWLQALDQYQSKEFANDRLGASRPFGYGPKRCIGEDLSYLEARLIISHLLFTFDMELVDGHSPGAEANLRWGLRADSESIQLYQVLMKPDLWVRLSKRNNVS

 

>CYP563D1 Mgr080 Mycosphaerella graminicola

42% to CYP563C1 Mgr027, 42% to 563A2

MGLLYATGVVLVLLTFAWLVRRRRRDGLRHIPGPWINTVSPFAFPYQVVKGFANVYLYALHEKYGPVVRIGPNEVSFAGPALEAIYGLRKGGALEKSRAWLGGVSPWKGFHGLGIARGDEHRRQRRALAPSMSKAALKSQEGIIQENVGQLMDRLRAEAAKSEDVNVAEWFTYFTFDMMGDVCLNQSFGCLDLGERTDWADSIIDLHISSSWLTVIQQIAGMETRFGRLLSKVLARLFVPRQLDKSRKLHFQKTMDATVRRMEAESSHPDAIYHILRSNKEDRIIAPREEIILNMTLFLSAGSETTSILLTTCSWLLISHRQVLDRVVREVRSKFQHISEVTLDTVTDAHLPYLHAVIHEAFRLFPPAGTGVGREVPPQGDTITGVYVPGGTTVRVAAWTVQRSAEHFHQPDTFQPERWLKPPPKEFENDRLELSQPFIIGPRQCLGAPLVMFESQLALSRLLLQFDISAPVTRRGIAENEKWNLSPTIRSIESYTVIKKPNTWVNLRPVPGMI

 

>CYP564A1 Magnaporthe grisea MG07550.4  40% to 65B1 AACU01000913 cont2.1400

MIIPTSWREALLYLGLGVVAIFACNAVYNVFFHPLRHFPGPKSHAMSRIP

YTYHWVRGDLVRHITALHREHGDIVRLAPDELSFGDPQAWGDIMGGGSRD

LQKWNGVFDIPNFLPTHVQNTTDKSRHRMLRKAMGPGFSDAALRAQEHIV

ASHVDQFIQKLRGRRGVVDLEVWYRFIVFDVIGDLAFGESFGCANGAEFH

PYIRQICETAVMISYLMAINMYPILRNVVNFLIDGVARRGFDAQAQTLMP

ALERRIKLQEQRLDLVDPLIGKMNEWKWTINDLIPHAIMIVGAGSETTAG

VLCGTTALLLEHPEAMARVTEEVRSAFSSDGEINLERVDSRLPYLRACID

EAMRLYPVPGCASLRITGDRDIICGVPMPPKTVVGLWPYAVYRDPKLWRN

PDEFHPERWLGDPEYINDARKAFNPFHIGSRDCVGRGLAVMELRLIMARM

IYNFDMHLMGAGMKEDSRSWVDRQKNFFIVWERVPLEVVLTPTKS*

 

>CYP565A1 Magnaporthe grisea MG07497.4  poor match 51% to MG03433.4

AACU01000908 cont2.1394

MISLVYITIGFVLYRIARIVYNLYLHPLAKVPGPKLYAASWFPRLWRQHH

KGCHHTDLVQLHEKYGPIVRVGPDEVSNSSAAAWDAIGGGSQQFMRDPHF

FIVSQLQTTGSKSFISLEKSEHHSIRKLMMPAFSNKTYLLHESLVNSWLG

KLTSHIAAKNGAELNIERMFVWFTFDVMGALSFGENFGCLDQEKDHQYLR

AAELGAPCLSIMQILLRFPATQGLYDVARKLPWMRLWNSLREMSDDSASR

WLETVHDEKRTDIMATVYRGMQNTKDPISRHEALDVASILVLSGAEATPI

LMTAMLWNLLSNPRCYERLCRDVRESGIINGPADFTAANVEKIPYMEAVM

LEALRLDTPFATTIPRIVPEGGAWVDGYWLPGGTSCGIPHYTAGHWAQNF

SDPDTFVPERWMASERPAKYESDKRGVFRPFSKGSLDCIGQRFVRHEVGM

AFVALLWNFDFELSDRSLDWKTANGAEHIRIIRKKRPLWVKAIPRNVPSA

V*

 

>CYP565B1 Magnaporthe grisea MG03433.4  poor match 51% to MG07497.4

AACU01000709 cont2.676

MALTFYMLLGIFVCYRIYRIIYNLYLHPLASVPGPKIYAASWIPRLWRQQ

VLGVHWKDLVALHEKYGPIVRTGPDEVSNASKQSWEDICGSRVFNRDLNF

LRVTQLSERGGFISPVKQEHRIVRRLLQPAFTDRTIDRHADILYEWVTKL

RLNVAAHGGEPVDVAQKLVWMTFDTMGVLSFGESFGCLDGNRQHPYLRAI

ELGAPFLSALQVVLRYRATSSLYGLLLRLPLMRFWNSLRATAEAKAARWL

DKADSSRGDVMSLIWAAMQDTANPLSAGQANDLASILCLAGAESTPVLMA

GMVWWVLSTPHAHARLAAEVRAAFAHASEIQVSNLSRLAYLDACIMEALR

QHTPFTVAIPRIVPEGGATVDGHFLPAGTVCGVPHWASAHLPTNFANPDA

FVPERWLPDPPAPYDQDKRDTFKPFAKGSMDCIGKRLVYHEVRMVMATLF

WHFDMSLCEQSVDWVAGANATTATWRPISTVFH*

 

>CYP566A1 Magnaporthe grisea MG01947.4  poor match. first exon is best guess

AACU01000379.1 cont2.368 revised 7/18/07

NZ_AACU02000388.1

MSFLYESSRDYTSL (0)

1605 IILLATILFAYNYWFHPIAHINGPFWAKATP

FWLMSSVSGHRHNNDIEALHAKY (1)

GPVVRVGPNELSFATEDALKAIHNPPPGQPMFTK

KGTIESLLASLIWSAPNLLSTHDKATHKRLRNALQPAFTAKALLEQEGIS

QYHIDKEVEKMLSVGSKDPKSVLNLSEEVAGMIWDIVGDLSFGEPLQRHQ

RDKYEHLKITYATGAPFLELCQFLTEIPIIGRLATLPIILTPFIYRKPRH

FLLINKLKQWIDDHDGGRKDFLTAIMSARETANLTHEEMLSNAALFVMVG

CDTTAATISALFYLLLKHPSSMARLTAELRTAFPRKGDVTATATLKLPYL

GAVIQETLRLLPPINGRGSHRISPGALVDGIYVPAGVMVSADPWSIGRSP

RYWAEPHAFRPERWVPGEQDEGPWRTDVRSAWRPFMVGPRVCIGREMALQ

SIRLMVSKTLVEMDLEMVNKDFVWERDAANDYVWYNFDILVKCKPRVRG*

 

>CYP566B1 Fusarium graminearum FG04590.1 AACM01000193 FGcontig1.193_scaffold2

AACM02000109.1 47% to 566A1 revised 7/18/07

MEDLLDQVVVELHRARSCLSDPLVFTQAAVIG (0)

SILFVFLLGLYNYFLHPIAHIKGPFLAAVTP (0)

ISLIRSVAGHRLNNDIKALHHKY (1)

GHVVRIAPNELSFSSERALRDIHNPGPDNHHYTKRGTSEDLILRFVFGA

NNILLVDEGEDHKRLRGALQPAFTAKAMRDQQDITHYHVQKTVERLLEAA

MDPSQTISLTKELNKLVWGNVGNLAFGEPATLEQLENHEKAKDLHAQIAP

ILEFFQYLNGNPILGRAARGLVGISRKVFGLSGNILGKDQLRRHIASQQG

QKNFLTAILGAKESSGLSFDEIHSNMLLLLMGGYDSSAASLSAIFYHLLR

EPQQYKRLQSELHHAYSSVNDITCNSLLSQPMLNACINESLRLVPPFNGH

GSHRVTTSGTMIDGVWVPAGTLISADFYSLHRDPSCWAFPDEYRPERWLK

EHQGPGTPFENDVKTAWRPFSLGPRVCVGREMALQSIRLAVSKIVYTFDM

TLANRDFVWDRDAGSHYMWHDFDIAVTLAKA*

 

>CYP566C1 Aspergillus nidulans AN2610.1 38% to 566A1 53 clan

revised 7/18/07 NW_101284.1, 79% to 566C2 Neosartorya fischeri, 79% to 566C2

MTLIESSPAQFTLVACSL (0)

GVAFLVLIVVYSYFFHPLARVPGPWTGAVFP (0)

309588 FWSISTLYRRRLNPDLAELHKIY (1) 309535

309472 GPVVRVGPNQLSFATIEAQKMIYNAKPTNNGSDEH

FGRDGTLQDVLLSMILGAPNIGSLSNRAEHKKMRK

RLLPGFTSNTIFEQEPLLRLHVDQLLHRLAQDAGLINLTSHFSRFLWDLI

GDFSFGEPLVPEKH (1)

ELRTDKLKSMVEIYQGCFPLLEAINHVVPEINAVIRLAFRLVPPATLRAVLPTATLRG (2)

CVDRQDGRSDFLTHIMGDKSRDPTELEL

SYDEVHSNATVLM (2)

IAGYKTTETSLSALFYRILSTPGVLKNLQSELFSNFQ

SIDGITGRKLLSLPYLNGCIYESLRLAPPVAGKFASRRSPGAVIQGFYVP

PGTEVFTETYTMQRSPLYWYAPDEYHPERWLKQSEGSPYAQDVHEAFKPF

SSGPRACLGREMALQTLRLTTALLAYRFDMEMVNEDQFVWEQDTESRMVY

SQYQIKAVVQEHLT*

 

>CYP566C2  Aspergillus fumigatus Af293

          GenEMBL XP_750865.1 also EAL88827.1 NZ_AAHF01000006.1

          89% to 566C2 Neosartorya fischeri, 79% to 566C1 revised 7/18/07

MGAQQFKAMSAISPEQVAIMACSL (0)

GTGLLVSVVIYNYFFHPLARVPGPWTGAIFP (0)

FWTMSSLYRRTLNPDLAELHKRY (1)

GPIVRVGPNQLSFATVEAQKMIYNAKPTHTGSDELFGREG

TLQDVLLSMILGAANIGSLSNRAEHKKMRKRLQPGFTSKALFEQESLLRLHMDRLLERLAQDTGVIDLT

EYFSTFLWDLIGDLSFGEPLVREKH (1) 1072139

1072086 AALHTDTLRSLVALYQGCFPILEAINYAVPQIEGVLRLAMQLVPTATLRAVLPSATFRD (2) 1071910

1071773 CIDRQDGRSDFITHITGDKSRNPTELELSYEELHSNATVIL (2) 1071651

IAGYKTTETSLSALFYRLLSTPGVLKKLQSELFRNFPSIDEITGKKLLSLPYLNGCVNES

LRLTPPVAGKFASRRSPGAIIEGFYVPKGTEVFTETYTMQRSPQYWHAPDEYRPERWVERGEGSPYAQDV

HEAFKPFSSGPRACLGREMALQTLRLTTALLVYRFHMKIVDEKRFVWEQDTDSRMIYSQYQIKAILQDRLT*1070978

 

>CYP566C2 Neosartorya fischeri NRRL 181 revised 7/18/07

NZ_AAKE03000024.1, XM_001258096, 89% to 566C2 = ortholog, 79% to 566C1

NFIA_055490 revised, removed two intronic seqs

Name revised from CYP566C3

189543  MDAQHLMTMSGISLEQVAIMGCSL (0)  189469

        GTGLLVSMIIYNYFFHPLARVPGPCTGAIFP (0)

        FWSMSSLYRRRLNPDLAELHKKY (1) 189182

189121  GPIVRVGPNQLSFATVEAQKMIYNAKP

        THTGSDELFGRDGTLQDVLLSMILGAANIGSLSNRAEHKKMRKRLQPGFTSKALFEQESL

        LRMHMDRLLQGLAQDTGVIDLTEYFSRFLWDLIGDLSFGEPLVPEKH (1) 188720

188664  GRRTDTLRSLVGVYQGCFPILEAINYAVPQIEGVLKLALQLVPPATLRAVLPSATFRD (2) 188491

        CINRQDGRSDFLTHIMGDK  188299

188298  SSNPTELELSYDELHSNATVLM (2)

        IAGYKTTETSLSALFY  188119

188118  RLLSTPGVLEKLQTELFSNFQSIDEITGKKLLSLPYLNGCVNESLRLTPAVAGKFASRRS  187939

187938  PGAIIEGFYVPSGTEVYTETYTMQRSPQYWHAPDEYRPERWFERGEGSPYAQDVHEAFKP  187759

187758  FSSGPRACLGREMALQTLRLTAALLVYRFHLKIVNEDRFVWEQDTDSRMIYSKYHIKAIV  187579

187578  QERLT*  187561

 

>CYP567A1 Magnaporthe grisea MG03819.4  poor match defective KYG seq motif

like CYP65s possible pseudogene? AACU01001030 cont2.747

MFHLFSDHVHWLGPLGVAVFFGTLAARCIYFLYFHPLAKYPGPRLAAVSY

LWYARKWLQGRYPWAIEELFETHSSEIVRVGPNGIAFRSPQAMHDIYMAT

VRNRETFVKTEFQDLGGKEPGITAERDPEEHRKVAKQLVPAFSPRAMLAQ

EQHVHRYVDLLVQQLDKHGGTSSKGVDMREWFDWLVLDIAGYMAWCHEFD

NLKNAETCFYLRESIKIGLSGTVGQVLRKFPLLLPLAPFFIPASVARTIP

TWMQFNRDIVAERLSKQEKLDGKDYFAWLLRDGSKPPSEDWLVAQSNVLL

AAGFDPLTNILTAAVHYLCKTPRALERLCAEVRGEFSTYSEIRADKLQSQ

KYLQAVLDESMRIHTNAAFGLPRYSPGALVDGEYIPKGVIVQNASFATTH

SPKYFLLPREFWPERYLGPDDRWYDQRFEHDHKKAFKPFSIGPRACPGRE

IGMMQARITLAKLVWTYDMELLNPEVDWDKESKLFLIWGRPEVRVRFRKR

VI*

 

>CYP567B1 Fusarium graminearum FG10695.1 AACM01000446 FGcontig1.446_scaffold7

MAATLIVFGGLLLLAWLVNIAYRSLFHPLAKFPGPKLAAVSDIWYAIKWT

SGRYPFIMEETHRKYGDVVRIAPNELSFATVQAYQDIYGHALKGKKKFVK

SNWYDTAGDHPGIVSVRDPKEHSRQRKYLSHAFSAKSLRGQEVLVHGYVN

LFLDQLR

KLGSSEGEGINIEEALNWLTFDIIG

DLAFGESFDAVANGKTHFWVSIIIDATYTSMLSALRKRVPLVN

LYLPFVVPKDAKATYQKHRALTREKMLKRLDMPNSEDRGDFFASLLRKGG

NEVPEPELLQQSNTLIVAGSETTATCLTGIVFCLLSNPSCLEALSNEVRS

RFQSDSEITGDATADMKYLSAVIEEGLRIFPPAPFGLPRISPGAVIDGHY

VPPGVTVSVDHWTTKHDRRYWKDPYSFIPERWIDEGFGDTKQASQPFSLG

PRACLGINLAYLEMRIIIAKMVYCFDWEL

PRLMVRFHPHN*

 

>CYP567B2 Fusarium oxysporum

85% to CYP567B1, yellow is possible intron but it makes the seq too short

This seq is missing in F. graminearum

FOXG_10874

MTASLSILGVFCASLSFTMLYIIYRVLFHPLAKFPGPKLAAVSDIWYALKWTSGRYPFIMEETHRKYGDVVRIAPNELSF

ATVQAYHDIYGHALKDKKRFIKGSWYDVAGDHPGIVSVRDPVQHSRQRKYLSHAFSAKSLRGQETLIHGYVDLFLGQLR

NLGSPGGAGINVEEALNWLTFDIIG (1)

DLAFGESFSAVAEGRSHFWVSLIIDASYAMMLSGLRKRVPLVNLYLPFVVPKDASAMHRKHMALTREKMLKRLEMPNSEDR

GDFFSHLLRKGGNKVPEPELCQQSNTLIVAGSETTA TCLTGIVFCLLSNLSCLEALSNEVRSRFQSDTEITGDATADLKY

LSAVIEEGLRIFPPAPFGLPRVSPGAIIDGHYIPPGVVVSVDHWTTKHDGRYWKDPHAFIPERWIDGGFGDTKQASQPFS

LGPRACLGINLAYLEMRIIIAKMVYHFDWEL

VNNEVDLFRDSKLYLLWKK

PRLLVRFHPHN*

 

>CYP567B2 Fusarium verticillioides

81% to CYP567B2 Fusarium oxysporum probable ortholog

FVEG_12172

MAASFSIILGLSCLL (0)

GVIWFLNAIYRLFFHPLAKFPGPKLAAVSD (0)

LWYGLKWTSGRYPFIMEETHRKY (1)

GDVVRIAPNELSFATVQAYKDIYGHATKDKKKFIKGDWYETAGDHPGIVSVRDPAQHSRQRKYLSHAF

SAKSLRGQETVIHRYVDMFIGQLRKLGSPEGSGINVEEALNWLTFDIIG

DLAFGESFSAVAEGRPHFWVSMIIDAAYFKALSALRKRLPIINLYLPFVMSKDAGEMH

RKHMELTRQKMMKRLQMPNSEERGDFFSHLLSKGGNDVPEQELCQQSNTLIVAGSETTA

TCLTGIVYCLLSNRSCLEKLTDEVRSRFHSEDEITADATAELKYLPAVIEEGLRIFPPAPFGLPRISPGAVIDGHY

ISSGVVVSVDPWTTKHDGRYWKEPYSFIPERWIHESFSDVKQASQPFSLGPRSCLGINLAYLEMRIILAKMVYNFDWELVNNDVDLFRDAKLFLLWKKPA

LMVRFYPRT*

 

>CYP567C1 Aspergillus nidulans AN1737.1 47% to 567B1 53 clan

MALESILSLPAATLAAGVAALVAMYWLASYAYLYWFHPLSKYPGPPLAAV

SELWYASAWTGGLWNRKIEEAHRRYGDIVRIAPNELSFATAQAFRDIYGA

PSKTRKLFPKSDRFYDNGHPNIAFVLDPEEHARQHKIFAPQFRPSAVRTQ

EPIVHAHVDFWVAQIAARGRSGAVPVDVSKWFEWLTFDIIGELTFGQSFN

ATRDNKSHPWVSILLDATYSGSIFNLRKRLAFVGPLLRWMPYISSTARAA

VESVSQHGAMTLAFTRKRIESGPARNDVDDFLAHAIRAGGMTDTELADQA

MVLLTAGAETSATALTATLWYLSQPAHAHCLERLRTEVRGAFASPADVTG

DATARLPYLNAVLEETMRLFPPSPVGPPRISPAGGETVDGTFVPGGVYVS

ADVWTIHHDARTVGARPDAFEPERWCDGQKPYTVPFSIGPRMCIGVTLAW

VEMRIALAKAVLAFDWELAEDAVGGSGDWVEEARLKQLWQKPPLMMRFRP

LEAKA*

 

>CYP567D1 Aspergillus nidulans AN3281.1 41% to 567B1 53 clan

revised 7/19/07 89% to A. clavatus CYP567D3

MSASWVDEPSSLLGIFEVVFVL (0)

KRHPNGSELQTLVYVSTRILYNIYFHPLAAFPGPLLARASM (0)

AWRIYHSMGGRFHRAIDEQHRRY (1)

GPVFRVSPNELSFASVASWKDIYGHRAT

GKPPLIKSKFYEIYGAGFGSLCIGSERDPEKHTRMKKSLSPAFSLRSLGD

QEVIVSQCVDRFVSRMGEPELNAGGLNMTKWYEMVAFDILGEL (1)

AFGESFHSIED (1)

GKPHFWSSLIEDHLYFITVADNFRHFPVVALIAKLLSPFLDSIRKKHTGYTRNKVAQ (2)

RIRSSSPRKDFMANLIGKVESNEMSREELTAHASTLV (2)

IAGGETVATFLAATTFYLLRDD

AVYQKLKAEIRGQFNTYEEITCATAQNLPYLQAVISEGLRIYPPGSQGFP

RISPGASIDGIWIPAG (0)

AEVYTSAWTVTHDEANFHDPHTFKPERWIDPDCADIKEASQPFSLGPRACLGRN (2)

VAYMEINLILARLIWTYDMELVNKSMDWESESSLHVMWSKPELKVRFRQARP*

 

>CYP567D2  Aspergillus oryzae

          GenEMBL BAE64652.1, AP007171.1

          99% to CYP567D2 Aspergillus flavus, 61% to 567D1

          14 P450 genes and 2 pseudogenes on this contig

MDRVVWLGLAVA (0)

286366 LTALYWVHLVIYNVFFHPLASFPGPFWARASF (0)

IWRMWHTSTGRVHRAIEFQHNLH (1)

GDIVRISPNELSFASVESWKAIYQPKSAPLVKSEFYEIYGSGFNSLCIGSE

RNPETHSRMRKSLAAAFSTKALLEQEDIIQGCVNDFIEGIRSQTTVNFTKWFEMLAFDILGEMAFGESFHCIEN (1)

SKSHFWCDMIEEHLYFITILDNLRRYPLIAAIGKTILPHLTVSVRNKHTNYSRRKVA

HRLQSSSPRADFMSRLIAKVEDEEMEMEELTAHASTLV

IAGGETVATFLAAVTYHLLSTPN

AYQKLRDEIRARYNHLSEITSTTALQLPYLQAVISEGLRIYPPGSQGFPRNTPPQGIVVKGTYVPGNVEV

YTSAWTVTHDARYFHDPYTFKPERWLDPNCTDNKDASQPFSLGPRGCLGRN

FAIVEMSLILCKLHFQFDAELVNPFQEWESASQLHVMWWKPDLPVRFIPCTRDH

 

>CYP567D2 Aspergillus flavus

AFL2G_04896, this model has a C-term extension that is probably wrong.

Model revised

MDRVVWLGLAVA(0)

LTALYWIHLAIYNVFFHPLASFPGPFWARASF (0)

IWRMWHTSTGRVHRAIEFQHKLH (1)

GDIVRISPNELSFASVESWKAIYQPKSAPLVKSEFYEIYGSGFNSLCIGSERNPETHSRMRKSLAAAFS

TKALLEQEDIIQGCVNDFIEGIRSQTTVNVTKWFEMLAFDILGEMAFGESFHCIEN (1)

SKSHFWCDMIEEHLYFITILDNLRRYPLIAA

IGKTILPHLTVSVRNKHTNYSRR

KVAHRLQSSSPRADFMSRLIAKVEDEEMEMEELTAHASTLV

IAGGETVATFLAAVTYHLLSTPNAYQKLRDEIRARYNHLSEITSTTALQL

PYLQAVISEGLRIYPPGSQGFPRNTPPQGIVVKGTYVPGN

VEVYTSAWTVTHDARYFHDPYTFKPERWLDPNCTDNKDASQPFSLGPRGCLGRN

FAIVEMSLILCKLHFQFDAELVNPFQEWESASQLHVMWWKPDLPVRFIPCTRDH*

 

>CYP567D3 Aspergillus clavatus NRRL 1

XM_001273850.1 89% to A. nidulans CYP567D1

ACLA_063960

MSASWGYEPTSWLDIFTVVFVL (0)

ERHANGTELQILVYVSTQSLYNIYFHPLAGFPGPLVARASL (0)

AWRIYHSMGGRFHRAIDEQHRRYGPVFRVSPNELSFASVASWKDIYCHRATGQLPLIKSKFYDIY

GAGFGSLCIGSERDPDKHTRMKKSLSPAFSLRSLGDQEAILSQCVDRFVSRMGEPELN

AGGLNMTKWYEMVAFDILGELSFGESFHSIEDGKPHFWSSLVKDHLYFITVADNLRHF

PVVALIAKLLSPFLNGIRKKHTGYTRDKAARRIGSSSARKDFMANLIGKLESNEMDRE

ELTAHASTLVIAGGETVATFLAATTFYLLRNDAAYQNLKAEIRGQFNTYAEITSARAQ

ALPYLQAVISEGLRIYPPGSQGFPRVSPGASIDGIWVPAGAEVYTSAWTVTHNEANFH

DPHTFKPERWIDPDCADIKEASQPFSLGTQACLGRNVAYMEINLILARLIWMYDMETV

NKSMDWESESSLHVMWSKPDLKVHFRQARP

 

>CYP567E1 Aspergillus nidulans AN3275.1 Missing N-term 147aa 53 clan

revised 7/19/07

MVPYGTCETMDHFRGQPALVLIGASALVGIITWIARLLIRAFVSHPLAHFPGPRLSALSN (0)

LNYSWSYMGGRQPYDILNLHAEY (1)

GSVVRVAPNELSFNTAQSWKDIYAPRKGRATFIKSDFYDGGNFADQASSI

VSERDPARHSAMRKFLSAAFSDRSLREQEGLVTRVIDDFIDQVGQRGNSKDGVDMTMWTNL

LTFDIIGEL (1)

AVVLESMGQAGLSDFLKRFPIIGRVFLKLNPRWLNKLMDGAIKHQTYTIDLVKR (2)

RIQQKTNRKDFMSYLLLERNSSQISDIQLAAHASDFV (2)

ATLKEDHSIAGSETTATCLATVIYYVGRNPRILKTLQEEVRSAFRNYEEI

NGQSTSSLKYLHAVCLEALRIFPPLALALPRVVPEGGEMIDGHFVPEK (0)

TIVSTNPLAASMDPANFDTPWAFCPERWIRLSEKDQLEASQPFSMGSRSCLGRG (2)

LAWLELRLTLAKLYYRYDLKLVDDELDWHRDAAMHLLWVKPKLMTQVLPRAK*

 

>CYP567E2   Aspergillus clavatus NRRL 1

           XM_001273844.1

           ACLA_063900

1     MDQFKGQPAWVLIGVSGLMGIMIWIARLLIQAFVSHPLAHFPGPRLSALSNLNYSWAYMG  180

181   GRQPYDILNLHAKYGPVVRVAPNELSFNTPQSWKDIYAPRKGRPTFIKSDFYDGGNFADQ  360

361   ASSVVSERDPAKHRAMRKFLAAAFSERSLREQEGLVTQVIDDFIDQVGQRGNSKEGVDMT  540

541   MWTNLLTFDIIGQL (1)

      AFGESFHGLETGNLHFWIAVVTESMGQAGLSDFLKRFPIIGHIFLK  720

721   LNPRWLNKLMDGAIKHQTYTIDLVKRRIQQKTNRKDFMSHLLAERNASQISDIQLAAHAS  900

901   DFVIAGSETTATCLATVIYYVGRNPRILKALQEEVRSAFGRPEEINGQSTSS  1056

1057  LKYLHAVCLEALRIFPPLALGLPRVVPEGGEMIDGYFVPAKTIVSTNPFAASLDPANFDA  1236

1237  PWVFCPERWLRLSAEDQLEASQPFSMGSRSCLGQGLAWLELRLTLAKLYYWYDLKLVDDE  1416

1417  LEWHGDVAMHLLWVKPKLMTQVTPRAK*  1500

 

>CYP567E3   Penicillium citrinum

           BD294642 PAT 04-NOV-2005

           DNAs related to the biosynthesis of ML-236B (compactin)

MEGFGSFLGIAMPLLVAFILKLGIWRVYFDQLSKFPGPGLSALTNVPYSMSYLSGRQPWE

MLSLHKKYGSVVRVSPTELSFSTAQSWRDIYGTQRGRAGFIKSSFYDGGNFADKAHSIVS

ERDPEKHSAMRRFLSTAFSDRSLREQEGLITSTIDKFVQKVGEVGSHPQGVDLTNWFNLL

TFDIIGDLAFGESFGGSLYIGKTHPWISVVLESMGQASLSDTIQRFPWMGRLYMRLNPEW

ARRLIAGSIKHESNTMDLVKRRIASKSPRSDFMSYLLRERSEFDQEVSDTQLAAHASDFV

IAGSETTATTLLVVAYYLSRYPEITRKLQKEVRSAFDSYEEINGSSTARLRYLHVICLEA

MRMVPPLPLGLPRVVPKGGAMVDGHFVIVSTNPLAASLDPKNFENPWTFDPDRWLQDGKE

QFGEASQPFSLGSRSCLGRSLAWLEMRVTLAKLHFSYDFTPVNENIDLQQDLRMHLLWKK

PELRFRVSPRK

 

>CYP567E4  Podospora anserina DSM 980

          NW_001914833 XM_001903508.1

          join(290518..290576,290629..290705,290761..291144,

          291257..291437,291504..291975)

          P450 adjacent to terpene cyclase

          49% to 567E1

MSTSIPFPSSLVPCTAPLVTQQIPHCLWFLGGRQPYKILELHNR

YGPVVRTAPNDLSFNTAQSWKDIYGSRQGHQAFVKSAFYDGGSFASRGVGSIVSERNV

DAHAQMRRYLSHAFSDRSLAEQEDIIARTMDIWVESLLKKGSRKEGIEMGKSFRIMTF

DIIGELAFGENFK (1)

ALNQGALADTLKRFPTLAWLAQGLLQKKIWELT

EDTKKNENFAIDMINRRIHRDELSRKDFMTHILQRRGSAQVSDLQLAAHASDFALAGS

ETTGTALSAIMYYLLRTPQVMLKLQKEIRGSFKTYSEICFRSTIGLPYLDAVILEGLR

MYPPVPLGLPRVVPDGGDTVDGHFLPAGS VVVYTHPVAASLSSANFKDPQAFKPDRWLDEHQGDIREASQ (0)

PFSLGTRACMGRKY (0)

MRTALAKVLWKYDLELVNTSLDWHRDSEMHTLWCKPALW

VRLRVLKE*

 

>CYP567F1 Nectria haematococca

fgenesh1_pg.scaffold_19000024

Necha1/scaffold_19:59238-60876

47% to 567B1, same gene as e_gw1.19.165.1, gw1.19.162.1

This gene model seems correct

MTISRAVLLLGISGSAV (0)

LVFWFLALVIYRLFFHPLAKYPGPKLAACTE (0)

LWFVRSWAGGYHPFVMRQVHEKYGDVVRVAPNELSFRSLAAHKDIYSQTPKGKAPFLKSKVFYNVGPSIK

HPDIVFTRDPEDHRVQRKALSHAFNSKGLRDAEETIQEHTKLFVEQIGKHAGPGTKGVDVTAVYNWLTFDVI (1)

GVLTFGESFESVSRWQSSGW

VTLLLNMAKHMTFLPVAERLSVPPFALGAVMPSDVSKNAAYHDKLTEEKINRRIGLIKSNDREDFFAFIL

REGNFDPVHLREQAKVLMLAGSETTATFLAMVTYFLLKNPVTMKNLQHEVRSAFSSAGEITGDSTNNLSY

LQAVIEEGLRMLPPSPIGLPRVCPGATIDGHFVPAGTDVSVDAYVLGRDDRYFPEPDEFRPERWIGDDTG

NEKDASRPFSFGPRACLGINLAYLEARIVLAHMVYAYDWEL (0)

PELYVRFHPRDV*

 

>CYP567F2 Fusarium oxysporum

59% to 567F1

FOXG_09562

MIAFNRKYERHNSEV (0)

FASWIIGVIIYRLYFHPLAKFPGPKIAACSE (0)

FWFLRSWASGYYVQNIGDLHRKYGDVVRTASNELSFRSPVALRDIYNHVSKDRPTFLKS

DTLYTVDPSVTRPDIVFTRDPQDHRLQRRSLAHAF SAKALRDNEESVQFHVKLLLERLGQ

NAGPGTGGANMSEVFNWLTFDVI (1)

GDLTFGESFDSLNQWRPSEWVTWLLGFISSLTFLPFINRFKIPMTLVVSVMPSYLKEKID

IHTTEKIKKRIGMGNSRDREDFFAYILRKESDNLDLVHLREQAKVLMIAGSETTANL

LAATTYYLLKNPDKLANLQAEVRSAFSARDEITGDAVAHLPYLNGVIEEGLRIFPPIPFG

PPRVCTGATIDGHHVPKGVVVSVDGFATTHDERNFIRPDEFLPERWIGEGFGDRKEASKP

FSLGPRGCLGINLAYMEARVTMASLVWKYDWEFVNQDLDWLADVRLHLLW

EKPKLMVRYHPRDEVATT*

 

>CYP567F2 Fusarium verticillioides

92% to CYP567F2 Fusarium oxysporum = ortholog

FVEG_14083 revised

MILFNRKFERHNSEV

FVSWIIGVIIYRLYFHPLAKFPGPKIAACSE

FWFLRSWASGRYINYMGDLHRKYGDVVRTATNELSFRSPVALRDIYNHVSKDRPTFLKSD

TFYTVDPSIPRPDIVFTRDPQDHRLQRRSLAHAFSAKALRDNEESVQFHVKLLLERLGQN

AGPGTAGANMSEVFNWLTFDVI (1)

GDLTFGESFDSLNQWRPSEWVTWILGFISSLTFLPFINRFRISMTLAVSVMPSYLKQKIDMHT

TEKVKKRVAMGNSRDREDFFAYILRKESHNLDLVHLREQAKVLMIAGSETTANLLAATTYYLLKNPDKLANLQAEVRSVFSSRDEITGDAVAHLPYLNGV

IEEGLRIFPPVPFGPPRVCPGATIAGHYVPKGVVVSVDGFATTHDERNFIRPDEFLPERWIGKGFGDRKEASKPFSLGPRGCLGINLAYMEARVTMASLA

WKYDWELASQDLDWLASVRLHLIWQKPKLTVRYHPRDEVAAT*

 

>CYP567F3 Fusarium oxysporum

59% to CYP576F1

FOXG_17536

MNFLSTLAALWVALSLTM (0)

ILVGLSGLCLYRLFYHPLAKFPGPKLAACSQ (0)

FWFLRAWTGGKYPFVMRELHDRYGDVVRIAPNELSFKTPQAYKDIYNHAVGHKTPFPKSKY

FYNRGASIKHPDIVFTIDRESHRSQRRSLSHAFSARALREAESTIQRHARLFVHQISQRG

NPGTGGVDMSAAFTWLTFDII (1)

GDLTFGESFESVANWEPDTYVALILEFIKHFTIIQAAKRLGISEGLLSLFIPGDLKDS

MAFHESLTKEKVARRIKLGDFNQRRDFFAHILRDGGFNRDHLAEQAKILLLDGSETTATFLAGVTYLLLKSPTALSKLQC

EVRSSFASLDEIRGDSLNQLPYLRAAIEEGLRLFPPVPLGLPRTCPGAMIDGWYVPSGTEVSVDNFVMSHDAKSFPEPDEFR

PERWLEGQTGNNKEASRPFSIGPRACLGVNLAYLEASLILATMAYSFDWELVDKSLHWFE

EVELMSFWNKPQLFIRYHPRGSFHGA*

 

>CYP567G1  Phaeosphaeria nodorum SN15

          XM_001795181.1

          102355-104120

          45% to CYP567E1

102355 MMITALSWFSAYSVQTL

102406 SLCALIVFVANKVWKHLRHPLREFPGPRLAAWTDIPYCYWLLGGRQPFVLLQLHEKYGP 582

583    VVRIAPNELSFNTAASWKDIYGYRPGHRTFVKGEFYDGAAFVKSHATRSLINTKDPTEHG 762

763    KMRRYLANAFSDKSLREQESLVAQEVDKLVEKLGEAGLAKDGTDLQRWLNMATFDITGSLSF (1) 945

       EQTTDDPQMARHPTV

       AFILRAILTLSFVDTVRRFPWLDT

       VARIAMPGRIK (1)

       AQLHHASTKRQ

1267   LTVPERRIDKPSNRPDILTRIIDKKDEAGISDIQIAAHAADLL (2)

       TMLTFHRTAGSDS 1446

1447   TNTAMGTVFYYLLKRSDIMQRLQAEIRSSVESYNEITALSTNNLPFLIAVIREAMRIYPP 1626

1627   VPLALPRIVPEGGDTIDGRFVPAG (0)

       TGVSTHPFAACLDAKNFEDP 1806

1807   WIFRPERWLGKSEQNVREASQPFSLGTRGCIGQ 1905

1959   LAWIEMRTIVAKVIYAYDLKWLNPVMDWHRESQMHILWHKPQLMVRVVARQRT* 104120

 

>CYP568A1 Magnaporthe grisea MG07507.4  poor match AACU01000909 cont2.1395

MTVLELPSERLASPPIAVLTAALIVLAVVFKAVYNIFFHPLRSYPGPLLW

GASRFPYVVRFMQGKIPYDLKRLHAEYGPVVRIAPDELTYNCSKAWRDIY

GRNKQPNGETTLFNKDPILYSAVFADHKALIDSDGDEHRPQKRTLSYAFS

HQVLVERGHIITGCIDRMIEGVKAHLGSSPSVSAVVDICPWIENALFDLG

GQLTVGRDLNAIMSQGTVHPSLALFKSLFDLVQCYYLQLRRGPGILINAV

ESMPGFSGYDCVLPVKDVHDMILKRMADEKYAVADENSGDFASYLMSRAK

SEGLNDDKVWINCVHLILGTPEAMPSSIISTIYHLLTTPYAYAEAVREVR

AAFKSSDEITAQDVQNLPYLLACFRETLRRRPTIPGTLTRQCGKFVQGGT

IVGVNQFATSRSASNFHDADSYRPERWLDPRPAEFEGDDRDAFNPFGYGP

RKCIARNLAHLTTQTFLAKLLWEFDMELDESCRDWDDQGLYVLALRGPLL

VKLSRAAQ*

 

>CYP569A1 # name changed to CYP531B1

no sequence

 

>CYP570A1 Magnaporthe grisea MG03392.4  40% to CYP527A1 39% to 527C1

AACU01000700 cont2.665

MLNMSFSAGSSSSCAMLATAAIWGISAVVAYAVFARFRDWRRLKHIDGPS

LAGWTDYWMIRSQLSGRMNLDLADVVREYGPVARIGTNTIVCADVKELRK

IWAVRSTWKRPRWYLGLRIDPYSDNVFSLMDDKVHETLRSKLMPGYGGKD

VDGVHEIIDEQISLFVRLLEDKYLSVDGKDSEGKSFLPVDLARKVQFLTL

DIISSLAFGESFGNLVADDDALGYISTTEKSMPMLMFVTLVPWLTAVWQS

PRLRWAFPDARKMVGIGDVMAIARRVVGERYGEKPEVVKRDMLGSFVAHG

LTKGEAESETMVQIVAGSDTSATAIRSTLLFIITNPAVYSRLQAEIDAAA

AEGRISSPITDAEARALPYLQAVIREGLRMYPPATGLLPKVSSKDEILCG

KHIPAGTDVGWAVWPVMRDRGVFGDDADLFRPERWVEAAPEQWRVMDQTV

MMDFATGSRWECLGKTIAMIELNKAYVELLRRFDITLLDPSNPWHSFNAA

FFIQKNMNVKISRRKTPV*

 

>CYP570B1 Magnaporthe grisea MG01544.4  40% to 527C1, 40% to 535A1 AACU01000068 cont2.284

MSRIHQFSTVVPVSVHKGTVFILFGTIAALLLAHSIYKSWSRLRHIPGPP

GTGLSKWWMFRNAMGGSMHLALKEAVDKYGSLARIGPNTLVTNDPDVLRK

LWAVRSPYKKSDFYQAVRFDPVRDNLISMRDDDEHSVLRNKMAAGYAGKG

NEGLENVIDKGISAFIGLIERNYVSDETAFRPMDLARKVQYASLDIIGLL

GLGERFGFTDEDTDVHQYIKILEESMTSMIILTIFPTMAKLLQSRLFRRL

MPSERDKIGFGVFIGVAKQFVTDRLASANPYRRDMLGSFLAHGLTREEAQ

GELLLQIVAGSDTTATAIRMTMLYVMSSHVVYERLVAEIRRWAADSLAAT

GNASVPATDSEARGLPYLQAVIKEGMRMHPPVAGLMGVVVPPGGDVLCGI

NVPEGTEVGWSAFSLQKREDIYGFDAACFRPERWLEADQESLRTMNATWD

LIFKYGKWQCLGKPVALIELNKLFVELLRRYDFALVDAASALKSDFAGIF

LQSELMVKVTRAQ*

 

>CYP570C1 Fusarium graminearum revised AACM01000122.1 added C-term gc boundary after YVE

MAVAALINYLRDLRPSIAIAASLVLFLAAWTALSTWRQYIRLRQFKGPTL

AAFSKWWLVKKVGGGRAYLDFWEVTQKYGTIARVGPNDLLTDDPDLMKHI

LNVRTEYRRSDWYDGMRFNPGNNNILSHRDEDEHFKLRSKMSAGYGGREV

ENLEPKIDKNILSFISLLAKYAEAKKPMDLGRRAQFFTLDVISDLAFGEP

FGFLETDSDVYKYIQITEETLPAVMVTTVIPWLVKILSSPLFKSLLPSEK

DRLGFGKLMGIAKKVTAERFGPDKKVRKDMLGSFVAHGLTQKEAESEILL

QIVAGSDTTATAIRSTMLHIITNPNVYSKLRAEIAGTHYSEPIIPDSIGR

DLPYLQAVIKEGLRIFPPVAGLMSKQVPPQGDTWKGQFIPGGTKVGYCAW

GIFRREDIWGSDAGEFRPERWLESDPEKLKEME

GALELIFSYGRWQCLGRPVALMELNKIYVE (0)

LLRRFDFSICDPTKPWKVFNCGIFSQSELMVRVTER

 

>CYP570C2 Fusarium graminearum FG02929.1 AACM01000143 FGcontig1.143_scaffold2

MGFIQLDSRGFALVGLLVFVAYVVATLRQWARLRHFKGPALAGFSQLWLI

SCVGGGRTHLDLWEACKKYGDIARVGPNDLITSDPDLMKHMLNVRTLFQR

SNWYDAMRLDPTKDNVLSQRNDDLHASTRSKMVGAYSGKEVDNVETTVDT

NVERLIDLLDTKYIAKNKAFDFGYKAQYFTLDVISSLAFGEQFGDLETDS

DVNGYIKAMEESMPTIIVTTVMPWMMKLLQLPVFKSMLPSEKDKVGVGRV

MAIAKRVTADRFGPNPKEHRDMIGSFIARGLLQHEVESEILMQILAGADT

TATAIRATVLYILSTPRVIEKMRAEIDQARPSLPVITDSEARAMPYLQAV

VKEGLRIHPPVVGLMSKEVPAGGDTFKGKYLPGGTKIGYCAWGIFRRRDI

WGEDADEFRPERWLESSEEQLHLMEGTLELVFGYGRWQCLGKTIAQMELN

KVFVEQVFIIASPAELVV*

 

>CYP570C3 Nectria haematococca

e_gw1.7.150.1

Necha1/scaffold_7:1454491-1456289

86% to 570C1

This gene model seems correct

MSSVDGLRGLFDGVRPSIAIAGLLVGVLIFWSGISTWRQYNRLRAFKGPRLAGFSKWWLIRTVGS (1)

GRAYLDFWEVTKKYGSIARVSPNDLLTDDPDLMKHILNVRTEYRRSPWYDGMRFDPANNNILSWRDEDEHFKLRSKMSAG (0)

YGGREVENLEPKIDKNILAFIKLLRKYADEKKPVDLGRRAQFFTLDVLSDLAFGEPFGFLETDSD

VYEYIKTTEETLPMVMVTTVVPWLVKVLSSPIFKSLLPSEKDRLGFGRVMG (2)

IAKAITAERFGPDKKVRKDMLGSFVAHGLTQKEAESEILLQI (2)

IAGSDTTATAIRSTMLHIITSPHVYNKLRTEISKTSYSEPIIPDSIG

RDLPYLQAVIKEGLRIFPPVAGLMSKEVPPQGDTWKGQFIPGGTSVGYCAWGIFRREDIWGPDAGEFRPE

RWIESPPEKIKEMESALDLIFSYGRWQCLGRPVALMELNKIYVE (0)

LLRQFEFSICDPTKPWKVFNCGIFSQSELWVRVEPRVTES*

 

>CYP570C4 Fusarium oxysporum

84% to CYP570C2

FOXG_14304 Supercontig 24: 19205-19551   (-) strand same seq

FOXG_14867 Supercontig_25: 302853-303199 (-) strand

FOXG_14868 Supercontig 25: 303463-304548 (-) strand

Supercontig_25 302737-304548, joined the two models above

MSFLQQLPQIGLLQLAAVAGIIILICYVVSTIRQWARLRHFRGP

AIAGFTQLWLISKVGGGRTHLDLWEACKKY (1)

GDIARVGPNDLITSDPDLMKHMLNVRTRFQRSSWYDAMRLDLTKDNVLS

QRNDELHASTRSKMAAAYSGKEVDNIEMTVDENVERLLELLDTKYIAKGRPFDFGYKAQY

FTLDVISALAFGEQFGDLETDSDVNGYITAMEESMPTIIVTTVMPWMIKLLQLPVFKSML

PSEKDKVGVGKVMAIAKRVSADRFGPNPKVQRDMIGSFIARGLLQHEVESEILMQM (2)

LAGADTTATAIRATLLYVISTPRVVQAMRAEIDQAKPSLPIITDS

EARAMPYLQAVIKEGLRIHPPVVGLMSKEVPKGGDTFKGHHLPEGTKVGYCAWGIFRRPD

IWGEDSNEFRPERWLDCSQDQLRLMEGTLELVFGYGRWQCLGKNIALMELNKVFVE (0)

LIRRFDLAVVEPIKPWQSINVGVFLQSEYWIRGYKRAGLS*

 

>CYP570C5P Fusarium oxysporum

Supercontig_23 457233-458956 (-) strand

Not found in Fungal genome database

One stop codon, possible pseudogene

MGLIHLDGRAAALVGVVFVLWYVVSTIRQWAHLRHFKGPAIAGFSQLWLISRVGGGRTYL

DLWETCKKY (1)

GDIVRVGPNDLI

TSDPDLMKHMLNVRTRYKRSSWYDAMRLDPTKDNVLSQRDDDLHASTRSKMAAAYSGKEV

DNIETTIDENVERLLDLLDTEYISKDIPFDFGYKAQYFTLDVISALAFGEAFGDLETDSD

VNGYITAMEESMPTIITTTVMPWMIKLLQLPIFRSMLPSDKDKAGVGRVMAIAKKVAAER

FGPNPKVQRDMIGSFVSRGLLQHEVESEILMQM (2)

LAGADTTATAIRATLLYIISNPRVVQAMRAEIDNSKLSHPIVTDAEARAMPYLQAVIKEG

L*IHPPVVGLMSKEVPQGGDTFKGQYLPEGTKIGYCAWGIFRRTDIWGEDSDEFRPERWL

HSSVDSLRLMEGTLELVFGYGRWQCLGKNIALMELNKVFVE (0)

LIRRFDLAVVDPIKPWLSINVGVFLQSEYWIKGYERASLA

 

>CYP570C5P Fusarium verticillioides

99% to CYP570C5 Fusarium oxysporum = ortholog, only 1 aa diff

both species have stop codons in the same place, both are in ther EXXR motif

FVEG_13512

MGLIHLDGRAAALVGVVFVLWYVVSTIRQWAHLRHFKGPAIAGFSQLWLISRVGGGRTYL

DLWETCKKY (1)

GDIVRVGPNDLI

TSDPDLMKHMLNVRTRYKRSSWYDAMRLDPTKDNVLSQRDDDLHASTRSKMAAA

YSGKEVDNIETTIDENVERLLDLLDTEYISKDIPFDFGYKAQYFTLDVISAL

AFGEAFGDLETDSDVNGYITAMEESMPTIITTTVMPWMIKLLQLPIFRSMLPSD

KDKAGVGRVMAIAKKVAAERFGPNPKVQRDMIGSFVSRGLLQHEVE

SEILMQILAGADTTATAIRATLLYIISNPRVVQAMRAEIDNSKLSHPIVTDAEAR

AMPYLQAVIKEGL*IHPPVVGLMSKEVPQGGDTFKGQYLPEGTKIGYCAWGIFRR

TDIWGEDSDEFRPERWLHSSVDSLRLMEGTLELVFGYGRWQCLGKNIALMELNKVFVE (0)

LIRRFDLAVVDPIKPWLSINVGVFLQSEYWIKGYERASLA*

 

>CYP570C6 Fusarium oxysporum

Supercontig_2 5000713-5002479 (+) strand

Not found in Fungal genome database

MGFIQLDSRGFALVGLLVFVAYVVATLRQWARLRHFKGPALAGFSQLWLISCVGGGRTHL

DLWEACKKY (1)

GDIARVGPNDLITSDPDLMKHMLNVRTLFQRSNWYDAMRLDPTKDNVLSQRNDDLHASTR

SKMVGAYSGKEVDNVETTVDTNVERLIDLLDTKYIAKNKAFDFGYKAQYFTLDVISSLAF

GEQFGDLETDSDVNGYIKAMEESMPTIIVTTVMPWMMKLLQLPVFKSMLPSEKDKVGVGR

VMAIAKRVTADRFGPNPKEHRDMIGSFIARGLLQHEVESEILMQM (2)

LAGADTTATAIRATVLYILSTPRVIEKMRAEIDQARPSLPVITDSEARAMPYLQAVV KEGLRIHPPVVGLMSKEVPAGGDTFKGKYLPGGTKIGYCAWGIFRRRDIWGEDADEFRPE

RWLESSEEQLHLMEGTLELVFGYGRWQCLGKTIAQMELNKVFVE (0)

LIRRFDLSVVNPIKPWHSINVGVFLQSEYWIRGYRRIN*

 

>CYP570C7 Fusarium oxysporum

Supercontig_13

88% to 570C1

FOXG_10682

MAAASLLHRLRDVFGDVRPSIAIAGLLVVFVTLWSALSTLRQYNRLREFKGPRLAALSKWWLIKKVGGGRAYLDFWEVTK

QYGSIARVGPNDLLTDDPELMRHILNVRTEYRRSDWYDGMRFDPGNNNILSWRDEDEHFKLRSK MSAGYGGREVENLEPK

IDKNILSFMNLLGQYADEKKPVDFGRRAQFFTLDVISDLAFGEPFGFLETDSDVYKYIQITEETLPAVMVTTVIPWLVKV

LSSPLFKSLLPSEKDRLGFGKLMGIAKQVTAERFGPDKKVQKDMLGSFVAHGLTQKEAESEILLQIVAGSDTTATAIRST

MLHIITNPLVYSKLRAEITDTTFSEPIIPDSIARDLPYLQAVIKEGLRIFPPVAGLMSKQVPPQGDTWKGKFIPGGTKIG

YCAWGIFRREDIWGSDAGEFRPERWLESKGEKLREMENALELIFSYGRWQCLGRPVALMELNKVYVE (0)

LLRRFDFAICDPTKPWTVFNCGIFSQSELMVRVHRRD*

 

>CYP570C7 Fusarium verticillioides

FVEG_09330

90% to CYP570C7 Fusarium oxysporum = ortholog

Note: possible mutation TGA to CGA at normal stop codon extends gene

Also one frameshift = & in first exon, possible pseudogene

MAEASFLDHMREIVGDVRPSIAIAGLVAIFVVLWSVLSTWQQYNRLREFKGPRLAA

LSKWWLIKKVGGGRAYLDFWE &

VTKQY (1)

GSIARVGPNDLLTDDPELIRHI

LNVRTEYRLSDWYDGMRFDPGNNNILPWRDEDEHFKLRSK

MSAGYRGREVEHLEPKIDKNILSFMKLLGKYADEKKPVDFGRRAQFFTLDVISDLAFGE

PLGFLETDSDVYKYIQITEEMLPAVMVTTIIPWLVKVLSSP

VFKSLLPSEKDRLGFEKLMGIAKQVTAERFGPDKKVQKDMLGSFIAHGLTQKEAESEILLQT

VAGSDTTATAIRLTMLHIITNPLVHPTLRAEISKTHFS

EPVIPDAIGRDLPYLQAVIKEGLRIFPPVAGLMSKQVPPPQGDTWKGKFIPDGTKIGYCAWG

IFRREDIWGSDAGEFRPERWLESKGEKLRERESALELI

FSYGRWQCLGRPVALMELNKVYVK ()

LLRRFNFTICDPTKPWTVFNCGIFSQSELMARVHRRD

RIVESHLITATETYTMDGPQWTRWIPVCTVIVYFQLRLLRSLCL*

 

>CYP570D1 Fusarium graminearum revised AACM01000470.1

MSNTSGGSAGLLRDILQIVNHEPKIAIGAVVFCFVVIFIVNSFRAWYRLS

HVPGPFLGSFSRFWMLRGSIRTHLPEEMQAAVDKYGSLVRVGPNELITDD

VKLIKKIHSGRSAYTRGPWFESVRFEPGKDNLFSMRDEEGHKKLRNKMAA

GYSGKENPSLEGSVDSIIASFIALLETKYLSTDDVYRPVDFAQKSQFFTL

DVISDLAFGQPFGYLTKDEDVFDFLKITRAFFPFTVTMANVPWMISLLHS

RLFSGLLPKDTDKIGFGAFIGVAKEKVAERYAPGAAPHADMLGSFIKNGL

SQEQTSRESLLNVVAGSETTATTIRMIMLCILTNPLAYRRLQQEIDDAAQ

AGKISSPITDAEARRLPFLQATIQEGLRIKVPAAGPLYKEVPGEGDTING

MFIPGGTQIGISPFSVYLSKKVFGPDASVFRPERWLNAEPERLEVMAESV

GLVFSSGKWQCLGKPVAIMELNKIFVE (0)

LLRRFDFSIVKPEKPLDIFNAVSNCSLVAVCKLTR*

 

>CYP570D2P Nectria haematococca

gw1.28.270.1

Necha1/scaffold_28:406101-407808

Chr 14

MSAGQGLSPGLLREAFVALSPGTVILTAVIGLVVLFLADSLRT*YRLSHIPGPFWAGFSKA*MVRQSLKG

TQPYAIQAANKKY (1)

GSLVRIGPNELATDDPKLLRRMMASRSPYSRGP (1)

WYNALRFEPGKDNLFSMRDDNAHAKLRNKMAAG (0)

YSGKENESLERTVDTHIARLIDLIETKYLSTSRDYRPTDFAQKIQYFTLDVISDLAFGQPF

GYVDQDDDVFDFIKITRSFFPVTLVIANIPSLVSLLHSRLFRGLLPKESDKFGFGAFIG (2)

VANKKVAERFA

PNATQHSDMLGSFIRHGLTQEEASRESLLNVVAGSDTSATTIRVIMLSLLSNPVAYRNLQKEIDDAIKAG

KISSPVTDAEARKLPYLQAAIQEGLRMKAPAAGPLFKTVPKQGDTIDGKFIPGGTQISTSPFSIYHSKKI

FGNDAALFVPERWLEAEPEQVEGMSEVVNLVFSSGKYQCLGKPVALIELNKIFVE (0)

LLRRFDFAMVHPERPLHIINAVSNAPSWLVL*

 

>CYP570D3 fgenesh1_pg.sca_33_chr6_1_0000031|Necha2

97% TO 570D2P W/O Stops

MSAGQGLSPGLLREAFVALSPGTVILTAVIGLVVLFLADSLRTWYRLSHVPGPFWAGFSKAWMVRQSLKGTQPYAIQAAN

EKYGSLVRIGPNELATDDPKLLRRMMASRSPYSRGPWYNALRFEPGKDNLFSMRDDDAHAKLRNKMAAGYSGKENESLER

TVDTHIARLVDLIETKYLSTSRDYRPTDFAQKIQYFTLDVISDLAFGQPFGYVDQDDDVFDFIKITRSFFPVT LVIANIP

SLVSLLHSRLFRGLLPKESDKFGFGAFIG VANKKVAERFAPNATQHSDMLGSFIRHGLTQEEASRESLLNVVAGSDTSAT

TIRVIMLSLLSNPVAYRNLQKEIDDAIKAGKISSPVTDAEARKLPYLQAAIQEGLRMKAPAAGPLFKTVPKQGDTIDGKF

IPGGTQIGTSPFSIYHSKKIFGNDAALFVPERWLEAEPEQVEGMSEVVNLVFSSGKYQCLGKPVALMELNKIFVELLRRF

DFAMVRPERPLHIMNA

GIWLIEDFPLRISRRDSSPDA*

 

>CYP570D4 Fusarium oxysporum

82% to CYP570D3

FOXG_14514 revised

MTLEGDPSAGFLRDVLQSVTDHPRAVIATAVLGLVVLFVADTLRTWYRLSHVPGPFWAGFSKAWMVRQSFKGIQPYAIQQ

ANEKYGSLVRIGPNELATDDPKLLKRMMSSRSAYTRGPWYNALRFEPGKDNLFSMRDDDAHAKLRNKMAAGYSGKENESL

ERTIDEHIAKLINLLETKYLSTDKDYRPVDFAQKIQFFTLDVISDLAFGQAFGYMEQDDDVFDFIKITKSYFPVT LIMANIPSLVSLLHSKLFSGALPKESDKLGFGAFIG (2)

VANKK

VAERFAPGAQSHPDMLGSFIRHGLTQEQASRESLLNVVAGSDTSATTIRLIMLSLLSNPIMYLKLRNEIDDAIKAGSISS

PITDAEARKLPYLQAVIQEGLRIKAPAAGPLFKQVPPQGDEIDGKFIPGGTQIGQSPFAVYHSKEIFGQDASLFSPERWI

NADPAKYEAMAEVVSLVFSTGKYQCLGKPVAFIELNKIFVE (0)

LLRRFDFCMARPERPLHIMNAVSYLSMIDVAAANW*

 

>CYP570D4 Fusarium verticillioides

94% to CYP570D4 Fusarium oxysporum

FVEG_13355

MSGESDTSAGFLRDVWQAVAKHPRAVIATAVLGLVVLFVADTLRTWYRLSHVPGPFWA

GFSKAWMVRQSFKGIQPYAIQQANEKYGSLVRIGPNELATDD

PKLLKRMMSSRSAYTRGPWYNALRFEPGKDNLFSMRDDDAHAKLRNKMAAGYS

GKENESLERTIDEHIAKLIKLLEAKYLSTNQDYRPVDFAQKIQFFTL

DVISDLAFGQGFGYIEQDDDVFDFIKITKSYFPITLVMANIPSLVSLLH

SKLFSGALPKESDKLGFGAFIGVANKKVAERFAPGAESRPDMLGSFIRHGL

TQEEASRESLLNVVAGSDTSATTIRLIMLSLLSNPIMYLKLQKEIDDAV

KAGSISSPITDAEARKLPYLQAVIQEGLRIKAPAAGPLFKQVPPQGDEIDG

KFIPGGTQIGQSPFAVYHSKEIFGQDASLFSPERWINADPTQYEAMAEVV

SLVFSTGKYQCLGKPVAFIELNKIFVELLRRFDFCMTRPERPLHIMNAGI

WLIEDFPVRITRRGA*

 

>CYP570E1 Nectria haematococca

e_gw1.4.199.1

Necha1/scaffold_4:1300784-1302553

42% to 570D1, 39% to e_gw1.7.150.1

This gene model seems correct

MGLLADLSLTRLCLVAVSVVIAGLGTRQFRRWHRLRHIPGPRGAGWSTWWQLRGALSGHYHEHLKQAADQF (1)

GPLVRIGPNELLSTDPDVLRNMSAVRSTYTKGDFYMSGRIVPEVDNVVSERNEVKHKAMKAKMTPG (0)

YSGKENEGFGFEAGLDRQLLNFIALIDRKYASAPDETRPVDLAEKTQFFALDAIGDVSFGEPFGYLTQDEDLY

HYNEINASSLSAMDVVSVYPWLTKIVHRWPLKLLLPREGDQVGFGRLMG (2)

FATQFVRGRLAEGATPDKDMMQAHINNGMNEEELIQQVFISI (2)

IAGSNSSAHALRMIILSIITNPPAHASLLAEIRQHVSSVNTPISWAQIQ

TLPYLQAVVREGLRMWPPLAGLGFKQVPPEGDTINGFFVPGGTQVGQGFHAVGRSRLVWGDDADMFRPER

WLLADESELKRMIAAWDTHFGHGKYVCLGKPIALMEIHKAVFE (0)

LIKRYDFAIMNPEKPISIQASVFLFASDFWVRITRRNDPDIEGTK*

 

>CYP570E2 Fusarium oxysporum

78% to CYP570E1 Nectria haematococca

FOXG_01097

MGFLTDQSLTKICFRVIIVLLIAQSFRLVRRWSRLRHIPGPASAGWTSWWQCRGAFSGRYHEHLKNAADQF (1)

GPLVRIGPNEVLSTDPVVLRN

MSAVRSNYTKGDFYSSGRIVPGVDNVVSERDEAKHKFMRVKMAPGYSYKENEGFGFEAGIDRQLLNFISLIDRKYLSTTS

ESRPLDLAEKTQFFALDVIGDVSFGEPFGYLTKDEDLFQYNEINASSLPVMNVVSVYPWLGRVVHRWPLSLLLPREEDQV

GFGRLMGFARHFVRKRLAEGITMKKDMMQMHISNGMNEEELIQQAFISIIAGSNTTAHALRMIILSLITNPNAYRSLIAE

IRKVTSSVSNPISWAQTQTLPYLQAVVREGLRTWPPVAGLGFKQVPPEGDTINGFFVPGGTQVGQGFYAVGRSRLVWGED

ADVFRPERWLLAGEDRLRDMVAALDTHFGHGKYSCLGKPIALMEIHKAVFELFKRYDFAILNAERPIKTQTSVFLFASDF

WVTITQRDDEEN*

 

>CYP570E2 Fusarium verticillioides

93% to CYP570E2 Fusarium oxysporum = ortholog

FVEG_00415

MGFLTDQPLTKICLQVTLVLLVAQLFRVVRRWSRLRHIPGPASAGWTSWWQ

CRGALSGRYHEHLKNAADQFGPLVRIGPNEVLSTDPVVLRNMSAVRSTY

TKGDFYSSGRIVPGVDNVVSERDEAKHKFMRAKMAPGYSYKENEGFGFEAG

IDRQLLNFISLIDRKYLSTTSESRPLDLAEKTQFFALDVIGNVSFGEPF

GYLTKDEDLYQYNEINASSLPVLNLVSVYPWLGRVVHQWPLNLLLPREED

QVGFGRLMGFARHFVRKRLSEGVTTRKDMMQMHISNGMNEEELIQQAFIS

IIAGSNTTAHALRMIILSLITNPNAYRSLIAEIRKVASSVSNPISWAQTQ

TLPYLQAVVREGLRMWPPVAGLGFKQVPPEGDTVNGFFVPGGTQVGQGFY

AVGRSRLVWGDDADVFRPERWLLADEDRLRDMIAALDTHFGHGKYSCLGK

PIALMEIHKAVFELFKRYDFAIFNAERPIKTQTSVFLFASDFWITIIRRDDEEN*

 

>CYP570F1 Mgr016 Mycosphaerella graminicola

42% to CYP570C1, 41% to 570D1, 41% to 570B1, (borderline member of CYP570)

MGLLEAALSLVTATNIILLIITAYVAQTYLASRSLAHIPGPFLSNYTTIPLLWKTYRGTLYHDIGELIAKHGPLVRIAPG

RVITGSAKTWAKITAARSPYLRSDWNDAMRFAPGQDNVLSMRDEKAHSDLRMKMAAGYSGKENPDLEKTIDGVVMQFVKL

IEEKYLSDAEHFKPMDLTEKFHFFTLDTITALAFGESFGDLKDDNDKYDYLKEIEKSITSIIMLADVPWLWNFLERTNLM

SLLLPGEELGLGKATKIAKDLVDARFEGSSDGKPTIDRMDMMGSFLRHSLTRQQVESETVLQLIAGSDTTATAISTVFLN

VLTSPPIYQKLMREIDETCIPGLAIGDAKARTMIYLQACIKESLRIAPPVSGVFPKLVPPGGETIDGYFVPEGTKIGWSS

KAVTQNPELYGPDASCFRPERWILQTHGGDCESVDKLLQMERDNAMIFGAGRFKCLGQAVAVLELNKIVVELLRRFEMRT

CDSKKPLAYEFNIGIWIQHGVWMRVTKRERYSG

 

>CYP570G1 Mgr065 Mycosphaerella graminicola

42% to 570C3, 39% to 570D3, 41% to 570A1, 39% to 527D1

The two families CYP570 and CYP527 may have some overlap

MVHTLLFAAFALFALIVARAARRHAALSHFGGHWSVGWSRLWLLRTQGSGQMNLRFTKINQQFGSTARIAPNMLITSDPD

LMRRMNAVRSPFTRGPWYAALKLHPDRDNITSYTDERKHADIRSRMAPGYAGRENPHLEQDVDDALIKMHSLIAEKYLNR

PENDQFRILDLARICTFFTLDVISKIAFGEPFGYLENDEDPYGYLANLAQLLPALIVFGVYSEFANLMKIPLLKASLPKS

TDKRGLGRVMGFAAARVRERFGTKPIVRRDMLGAFINKGLTQGELESETLTQIIAGSDSTASSLRLTLHFITTSPPILER

LLAEANAGIEAGRISRPVIKDQEARGLPYLQACIKEGLRMYPPVTGLLAKRVPDEGVHIDVDGVEKYIPGGVQIGWNSWA

MMRREEIFGKDVEMYRPERWLLRQGGGAAEVARAKRMTETVELCFGYGRFGCLGRGVATMELNKAVLETLLRFSLQPCSL

SKPFDEKVVGFYIHDNMNFIATERRTDSGRDVLELTSKAGDGADAAEASAIGGAYEE

 

>CYP570G2 Mycosphaerella fijiensis

71% to CYP570G1 Mycosphaerella graminicola

estExt_Genewise1.C_260099

MVAAAFLPLFAIVLLLIARKIRQFYALNHFAGHWSSGWSRLWLLRTQSSGRMNKIFTELNNKYGSTARIGPGMLITTDPE

LMRRMNAVRSTFTRGPWYAALKLHPERENITSYIDERKHADIRSRMAPGYSGRENHHLEEEVDDRLMKMHTLINDKYLTR

PDEGVYKTLDLSRVTSFFTLDVISQIAFGQAFGFLDHDDDPFGYLKNLSEFLPAILVFGTYTELTNILQLPLLKAALPKN

TDKRGLGRVMGFAAERVRERFGSKPVVRRDMLGAFINKGLTQDELESETLTQITAGSDSTASSLRVTLHFISTSPPILHR

LLSEAKAGISAGKISRPIIKDSEARKWPYLQACIKEGLRMYPPVTGLLAKRVPDDGTVIEVDGVEKFAPGGTQIGWNSWG

MMRHQTIFGPDVEIFRPERWLPRDESELEKQRIQTMTETVSLCFGYGRFGCLGRGVATMELNKAIIETLLRFNLQPCSLA

KPFDESVVGFYVHENMNFVATERKDLGEGGGEVVFEGEVDAGAIAGAYEE*

 

>CYP570H1 Mgr068 Mycosphaerella graminicola

44% to CYP570C1 new N-term from JGI model fgenesh1_pg.C_chr_1001460|Mycgr3

MDSVSGLNNSFGFTAIDARRHLNGIGVDVMRSDDILKITKQPFRALTVLLLSAILLSLVARVSRRYRHLAEYRGPGIAAY

TRLWICRTIASGNSAKI

MVDINKQYGPIARIGPNHLLTSDPELVKHILAARSHYTRGAWFDSIRIDPEVTNIVSERDTGRHNHLRHQMSGGYGGKEV

ENLERDVASRVMEFVSWLEERVTECGNGKQPIDLARPIQYLTIDIITHLCFGYPLGFVRENRDLFNFLQTIETQLPIVQH

FSVILELNTLLRTLVKVPFLRPFITPSARDKSGIGVIMGLSREVIDKRYAPGAPAERDMLGAFKKHGITADEAVTEVSIS

LVAGSDTTATSMRAILLMIISNPRVYGKLQQEIDDFAAKGMLNSPVADEQSRQMPYLQACIKEGLRRYPPITQLRERVSP

PEGDIFHGHRIPPGTFVGINAWGLQLDPVYGPDADVFRPERWLEAEPEQLVAMTQVHGLIFGYGNTKCLGIPIAMMNLNK

FFVEVLRRYDVALADATQPWKSLCYGIFFQKDFNVVISRREEAV

 

>CYP570J1 Histoplasma capsulatum G217B

48% to CYP570A1   Magnaporthe grisea

HCB00160.1

MRLLLLLVSSLAVAYLVVRRLQTWYRLRHIPGPRWCGWTDIWILWRAWTGSLYEDLGDLCQQHGPLVRVAPNYVVCGNPA

EVRRMWGVRSEWDRSPWYKGFQLDPPRDCTLSMRDNALHTVLRSKLAPGYSGKGIESLPESVDAQIAKFIRLIETKYLST

DTELRPVDIARKVQYLTLDVISTLAFGRTFGFLDEDGDLFDYIKTTEESLPLMQMIALLPWLVNVLQSRLFKAFLPSHTD

VVGVGKVMGIAKEVVSERYGDSKITKPDMLGSFVAHGLSQRDAEAESLVQIVAGSDTTATTLRISLLLLTTSPRTYATLV

SEIDTAISRGNISSPVTEAEARSLPYLQACIKECFRFWPPITGIMPRICPHEAEVCGVKVPGGTNVGWSARAVLRDKEVF

GEDAEVFSPARWLERADSKEGNRDGETERLGRMERTVELVFGQGKWGCLGKPISLLEINKVLVELFRRFDFAILNPAHPM

DTFNYGVSMQSGLFMKISRRTHI

 

>CYP570K1 Aspergillus clavatus

49% to CYP570C1 Fusarium graminearum, 47% to CYP570D3 Nectria

ACLA_061370 revised

MILPSFKIGETSVSVQVLLVTLLGLSLVSLAQRFVAWRRLSHIPGPFLASFSNLW

LAWHAHRGTLPWAIKDVTDKY (1)

GPLARVGLNVAVNANPESVVKILQPRSGFIRALDYQAARFRPGSDNILSMRDEKAHKTLKLKTTAGYNGK

DVEGLEGQIDRVVESFVDLIERKYLSTPSDYRTVDLARITQYFTLDVIMSVAFGKNFGHLESDSDVYRYLSMTESFMPVV

AIVLVYPWLCNVLESKFLKMMAPKDTDKQGMGKVMGFAKEVVSERFGPNRIVRKDMLGSFIAHGMTQEEANSETLVQIVA

GSDTTATAIRAIFLHVISSPHIYSTLRKEIDNAGPISSPITKAEAENLFYLQAVIEEGLRFWPPIGLLASKLVPNGGATI

NGFFMPEGSNIGVSIMGIQRSKDIFGEDADEFRPERWLNIGEGEAEKAREKKMRGTVDLVFASGKYSCPGRQVAMMELNK

IFVEVLRKYDITLVNPSQPWTSFYAGVWMQKNLRVRFSKRTDSVIA*

 

>CYP570L1P Mycosphaerella fijiensis

40% to CYP570F1 Mycosphaerella graminicola

e_gw1.17.140.1

frameshift = &

MSTSISLYSELLLIGVLGLATAFYLASWARSYRRLSHI &

GPPGWGLTVMPWVLVHLKPDM

MDQYYQLSKRYGPSVRVGPNTVITFIRKMSAVQSPCRRSMNYYAMRLNPGKDHLFSTRNEVAHNDLRKTMTAHYSGKENL

SLENDVDESILELCHLIDNKYSSTAGNIKPMHLARKISFMVLDIISKVAFDAKFYDLTDDRDNHGHIAEIENLLPSITWI

APVAGLVKFLTDIGLLQMAARFAGGRAGVEKKRFQPDGRPKDEMRSDMLGSFIRHGLSHERAKEEAILNLTAGSDTTAST

IRATMLNIMASPRLYRLLTAEIDDAVARGALPPEGKIVSANQAHELLPLLQATIKEGLRWYPAVAAELSKLTPPQGDTIC

GYYVPGGTKVGTSMKVLYRNEELYGPDAEAFRPERWLLSSESRSPGTLEPFWDTRTSAMTLNAKGCLAFPPRTCSTHEWF

FDIARQCSDDTYIHSSISTTDRPHTSNISGLPSSNPLKSVDSDFFFLFQKTWYLKPLRPRSPQPA*

 

>CYP571 # name changed to CYP535

no sequence

 

>CYP572A1 Magnaporthe grisea MG04345.4  poor match AACU01001098 cont2.822

MAVTSLSTLALFVPILVIGSLAWRIIHSLYFHPLSKFHGPWYAASFSIVH

ALASTLKVEHEWLLQLTKRYGASKPIRIAPNLLLFPQPSAVKEIYWDSKL

NTKGLFYGSGALGPPHLFTTLDGDQHKELRKAIGGPHYTIGTLKNHWEPR

FDDLILTALEKWTEKSERGETIQLCDKVAEFAADVMTMVSFSEPWGFVRN

DRDERELLKRWRDSLPYFGFVNRFRWFRDVVMKSSWGVYFLPSSSDNTGM

GFFMREADRQVTIREYRMKNQQYSQECPDFLQHCLEARIGTKPLSPVQKR

AHITLLFQAGADTTGTALGCTFRFLVTNPDVMSRCQAEIDAADQAGKLSS

PVVQYEETRTHLPLVVACIRESLRLHPPAPTLFPRIVPRAGGGGLGAKKT

SSSGGFKTIGDVVLPPGFEVTTHPYVVQRDPVLYAPDPEVFRPERWLEAS

PEKLSEMEAGQFTFGVGPRVCLGKDVAYMELYKLIPQVLRTFDMDLQRPG

QFVVAGGVAFNNDFAVKLRVRKSGETFMHD*

 

>CYP573A1 Magnaporthe grisea MG05215.4  poor match AACU01000797 cont2.962

MAILSPVGLVLIPLAYIVYKILQQIAKYRLNHPLNPFPGPWLASVTRLWI

TYHNVKADECQTFRELHRRHGPVVRITPTMLHVSDAVKLPEIYSRNADKS

QHYITGSFGTTESLFNMQDHKVHAHFRKIAASPYSFSNVKRMEPLLDSNI

DRWIHRLDEQFASTRQRFDFAPWAVYMAYDIISEVGFGQPFGFIEQGRDV

EGLIKGFHDGLVPFGIMARLWPFTNWVKTTFLGRYLVASPEQDSGIGTLM

RLRDRLITQRIADNEKSGGNSHARVDLLQTFLEARDDEGRPLDVAHVKAE

ILLVLLAGADTTGTTFQAIVMHVASRPDVYERMMAEVDAAAAAGKLSSPT

PRHEEVQAHCPYYVACVRETMRLNPAAPNIFPRLAPAGGIDLYGVRVPGG

TELTCNPWIVHRDPAVVGDDPDHFRPERWLEDGDRAKAMLRYSMSFGYGA

RECLGKHIAMELFKAPIRFFQTFKPDILDKTNPGRYVVKGGVSYFEDMWI

KIERRVRVE*

 

>CYP573A2 Fusarium graminearum FG03008.1 AACM01000145

FGcontig1.145_scaffold2, C-term revised

MALLSIVNVALLGVAYFVACAVWQVVKYRFLHPLAKFPGNFWGSVTRLWI

TYHNVEADECETFQELHKKHGPIIRITPTMLLVSDATELPKIYNRHANKS

KHYITGSFGKDESLFNMQDSVMHAKYRKVAAPLYSLTNIKKMEPLIDNNM

SAWMSRLQRDFAATNKPFDFAPFSVYLAYDVISEVGFGAPFGFVKEGKDV

EGLIQGFHDGLTPFGIMARLYPFTNWVKSTFLGKYMVASPEQDSGIGILM

RFRDRLIEQRFKDIENGSTGGRIDLLQTFIEARDEDDKPLDINYIKAEIL

LVLLAGADTTGTAFQAMMVHILTNPSVYKKLLAEIDEATAAGNLSEMPQY

DEVVEHLPYYIACVKEAMRLTPSAPNIFPRIVPQGGLEICGHFVPEGTEV

TCNPWLVHRDPNIYGDDAEIFKPERWLDADKAKIYNKYSMGFGYGPRVCL

GQDIARMELYKGPLLFLRSFNVEWVDETKRGKYVINGG

VSYFEDMNIKISRRDAAA* 123417

 

>CYP573A3 Aspergillus nidulans AN7773.1 57% to 573A2 53 clan

MGLGELALSQLTLANVVLGGIAYIVLKFIYQIVYYRFFHPLSVFPGPFWG

SVTRLWIAWHNLKETELPTIYGLTKKYGPVVRVTPTLLLVSDPTKLPEIY

HRNADKTGHYITGSFGETESLFNMRSHKTHAAFRKHAAGPYSFSSVKRME

PLIDARIRDWSNKLNEKYVQTGEAFDFSWWAVYMAYDIISEVGFGAPFGF

VEKGEDVGGLIQGFHDGLPAFGLLARLHPFTSWMKTTFLKKYLVAKPEDD

SGIGVLMRFRDGLIEKRVQDLKSNKDIGRIDLLQTFLEARTEDGQPLTMD

YIKAEILLVLLAGADTTGTVFQAMIYYLLTNQSVYERMMDEVDTAIRKGL

ITDDVPQYIEIQEHLPFFVAIVRETLRMCPPAPNIFPRYVSEPGLDLYGK

VAPAGTEITGNPWIMHRDEAVFGKDAEEFNPDRWMDPERAKIMNKYLFTF

GYGARVCLGRDIAMMELFKGPLQFFRYYKPYPVQGKPEAKFVIKGGVGFW

RDMWVKIEKRPLVKPE*

 

>CYP573A4 Nectria haematococca

e_gw1.23.293.1

Necha1/scaffold_23:206165-207796

MAVLSFANLALLGVGYVVLSFAYQIIKYRFFHPLAKYPGNFWGSVTRLWITYHNVKADECATFQKLHQQH (1)

GPVIRITPTMLLVSDATKLPQIYSRGASKSKHYITGSFGKEESLFNMQDATVHAKYRKIAASPYSFSNIK

RMEPLIDINIQAWMDKLQETFASTQKQFDFAPWAVYMAYDIISEVGFGAPFGFVEAGKDVEGLIKGFHDG

LTPFGIMARLYPFTNWVKGTFLGKYMVASPEQDSGIGTLMRFRDRLIQQRFKDIEDGTTSGRIDLLQT (2)

FIDARDEKGEPLDINYIKAEILLVLLAGADTTGTAFQAMMVHILSNPCVYEKLLAELDEATRANKLSDMPQY

EEVLAHCPYYIACVKESMRLNPSAPNIFPRLAPKGGLELYGEFIPEGTELTCNPWLVHRDPNIYGEDAQV

FRPERWLDEEKAKIFNKYSMGFGYGARVCLGQDIARMELYKAPLQFFRTFKFELADTKKPGTYVVKGGVS

YFEDMYIKIQRREELGRTA*

 

>CYP573A5 revised Aspergillus oryzae RIB40

AP007157.1a genomic DNA, SC023

75% to 573A3, 60% to e_gw1.23.293.1

79% to CYP573A6 Aspergillus fumigatus

complement(join(116142..116234,116333..117206,

117277..117401,117462..117649,117710..117944))

revised 3/17/2009

Supercontig 8: 116142-117944 (-) strand

MVLAGEDLVAMFTVTHVILALGAYVALRFAYQIVYYRFFHPLSVFPG

PFWGSVTRLWIAWHNLRETEVPTVYALTKKY (1)

GPVVRITPTLLLVSDPTKLPDIYHRNA ()

DKTGHYITGSFGETESLFNIRSHKTHAAFRKHIAGP (0)

YSFSNVTRMEPLVDARIDDWLNKLDETFASTGAKFDFAWWAV ()

YVAYDVISEIGFGAPFGFIEQGKDVGGLIQGFHDGLPAFGLLARLHPFTSWMKTTFMKKY

LVAKPEDNTGIGVLMRFRDRLIEQRLREIAEGKDVGRVDLLQTFIDARTEDGKPLDMDYL

KAEVLLVLLAGADTTGTVFQSLVNHLLVNQEVYERMMEEIDTASRKGLIPETIPQYDAIQ

EHLPYYVACVRETLRLNPPAPNIFPRYVSEPGIELFGKFAPAGTEVTGNPWIMHRDTALF

GEDAEEFRPERWLDPEKAKLYNKYIFTFGYGTRVCLGRDIAMEELFKGPLQ (0)

FFRRYTPSIATDKPSARFEVKGGIGYWRDMWVSINRRPAVKPL*

 

>CYP573A5 Aspergillus flavus

99% to CYP573A5 Aspergillus oryzae, only 1 aa diff

AFL2G_03949

Note> end is n a sequence gap

MVLAGEDLVAMFTVTHVILALGAYVALRFAYQIVYYRFFHPLSVFPG

PFWGSVTRLWIAWHNLRETEVPTVYALTKKY (1)

GPVVRITPTLLLVSDPTKLPDIYHRNA ()

DKTGHYITGSFGETESLFNIRSHKTHAAFRKHIAGP (0)

YSFSNVTRMEPLVDARIDDWLNKLDETFASTGAKFDFAWWAV ()

YVAYDVISEIGFGAPFGFIEQGKDVGGLIQGFHDGLPAFGLLARLHPFTSWMKTTFMKKY

LVAKPEDNTGIGVLMRFRDRLIEQRLREIAEGKDVGRVDLLQTFIDARTEDGKPLDMDYL

KAEVLLVLLAGADTTGTVFQSLVNHLLVNQEVYERMMEEIDTASRKGLIPETIPQYDAIQ

EHLPYYVACVRETLRLNPPAPNIFPRFVSEP

 

 

>CYP573A6  Aspergillus fumigatus Af293

          XP_748848.1 also EAL86810.1

          68% to 573A3, 95% to CYP573A6 Neosartorya fischeri

          AFL2G_03949

          revised 3/12/2009

MGLREEILARLTLSHVVLGLVAYVLLKFVYQIVYYHFFHPLSVFPGPFWGGVTRLWIAWHNLRETELPTVYALTKKY (1)

GPVVRITPTLLLVSDPTKLPDIYHRNADKTGHYITGSFGETESLFNMRSHKTHAAFRKHLAGP (0)

YSFTNVKKMEPLVDIRIQHWIDKLNEKFAQTGEIFDFSWWAVYMAYDIISEIGFGAPFGFVEQGQDVGGLIQGFHDGLPAF

GLLARLHPFTSWMKTTFMKKYLVATPQDDSGIGVLMRFRDRLIDQRFRELETKKDIGRIDLLQTFIEART

EDGSPLDMEYLKAEVLLVLLAGADTTGTVFQALVHFLLTHRDAYRRLMEEIDAATAQGLISDPVPQYDEI

VEHLPYYVACVRETLRLNPPAPNIFPRYVSEPGMDLYGKFAPAGTEISGNPWIMQRDKKLYGEDAEEFKP

ERWLDAERAKRYNKYSFTFGYGARVCLGRDIAMMELYKGPLQ ()

FFRRYHVQTVTDKPKARFVIKGGVGFYRDVWVTIDRRLPQKA

 

>CYP573A6 Neosartorya fischeri

95% to CYP573A6 Aspergillus fumigatus = ortholog

NFIA_027910

MGLGEEFLARITLTNVVLGLVAYVLLKFAYQIVYYHFFHPLSVFPGPFWGGVTRLWIAWHNLRETELPTVYALTKKY

GPVVRITPTLLLVSDPMKLPDIYHRNADKTGHYITGSFGETESLFNMRSHKTHAAFRKHLAGP

YSFTNVKRMEPLIDIRIQHW

IDKLNEKFAQTGEIFDFSWWAVYMAYDIISEIGFGAPFGFVEQGQDVGGLIQGFHDGLPAFGFLARLHPFTSWMKTTFMK

KYLVATPQDDSGIGVLMRFRDRLIDQRFRELEEKKDIGRIDLLQTFIEARTEDGSPLDIEYLKAEVLLVLLAGADTTGTV

FQALVHFLLTNPDAYRRMMEEIDAATAKGLISDPVPQYDEIVEHLPYYVACVRETLRLNPPAPNIFPRYVSEPGMDLYGK

FAPAGTEISGNPWIMQRDKKLYGEDAEEFKPERWLDAEKAKLYNKYSFTFGYGARVCLGRDIAMMELYKGPLQFFRRYNV

QTVNDKPKARFVIKGGVGFYRDVWLTIDRRLLEKA*

 

>CYP573A7 Aspergillus niger

fgenesh1_pg.C_scaffold_11000284|Aspni1

77% to CYP573A3

MSVGEDLLSKVTVTNVILGVVVYVVLQFVRQIVYYHFFHPLSKFPGPFWGGVTRLWIAWHNLRETELQTVYDLTKKYGPV

VRITPTLLLVSDPKKLPDIYHRNADKTGHYITGSFGETESLFNMRSHKTHAAFRKHIAGPYSFSSVKRMEPLLDAHIEHW

LQKIDERFASTGAGFDFSWWAVYMAYDIISEVGFGAPFGFVEQGQDVGGLVQGFHDGLPAFGLLARLHPFTSWMKTTFMK

KYLVATPQDDSGIGVLMRFRDRLIDQRLRDLEEKKDIGRIDLLQTFLEARTEEGKPLDMDYIRAEVLLVLLAGADTTGTV

FQALVHNLMTHTEVYERMMAEIDDAARKGLISPMAQYQEIVEHLPYYAACVRETLRMNPAAPNIFPRYVSEPGIDLYGQF

APAGTEISSNPWIVQRDEGVFGADANVFNPERWLDPERAKVMNKYMFTFGYGTRVCLGKDIAMMELFKGPLQLFRKFKLH

SIAGKPSAHFVIKGGVGFWRDMWVTVDKRGAVKAT*

 

>CYP573A8 Fusarium oxysporum

86% to CYP573A2   Fusarium graminearum

FOXG_12028

MAVLSFVNIALLGVGYIAFCALWQIVRYRFFHPLKKFPGNFWGTVTRLWITYHNVKADECATFQELHKKHGPVIRITPTM

LLVSDATQLPKIYNRHANKSKHYITGSFGKDESLFNMQDSVMHAKYRKIAAPLYSLTNIKKMEPLIDSNIENWMARLQRE

FASTNKPFDFAPWAVFMAYDIISEVGFGAPFGFVKEGKDVEGLIQGFHDGLTPFGIMARLHPFTNWVKSTFLGKYMVASP

EQDSGIGTLMRFRDRLIDQRFKDIENGATDGRVDLLQTFIEAKDEDGNPLDINYIKAEILLVLLAGADTTGTAFQAMMIH

ILTNPSVYKKLMAELDDATIAGKLSEMPQYDEVVEHCPYYIACVKESMRLNPSAPNIFPRIAPPGGLEICGQFVPEGTEV

TCNPWLVHRDPNIYGDDAEVFKPERWLDEDKAKVYNKYSMGFGYGARVCLGQDVARMELYKGPLQFLRSFKVDWVDEKNR

GTYVVKGGVSYFENMNITIQRREVVA*

 

>CYP573A8 Fusarium verticillioides

97% to CYP573A8 Fusarium oxysporum

FVEG_10612

MAVLSFVNVALLGAGYIAFCGLWQIIRYRFFHPLRKFPGNFWGSVTR

LWITYHNVKADECATFQELHKKHGPIIRITPTMLLVSDATQLPKIYNRHANKS

KHYITGSFGKDESLFNMQDSVMHAKYRKIAAPLYSLTNIKKMEPLIDS

NIENWMARLQREFASTNKPFDFAPWAVFMAYDIISEVGFGAPFGFVKEGKDV

EGLIQGFHDGLTPFGIMARLHPFTNWVKSTFLGKYMVASPEQDSGIG

LMRFRDRLIDQRFKDIENGATDGRVDLLQTFIEAKDEDGNPLDINYIKAEIL

LVLLAGADTTGTAFQAMMIHILTNPSVYKKLMTELDDATLAGQLSEMPQ

YDEVVENCPYYIACVKESMRLNPSAPNIFPRIAPPGGLEICGQFVPGGTEV

TCNPWLVHRDPNIYGDDAEIFKPERWLDEDKAKVYNKYSMGFGYGARVCLG

QDVARMELYKGPLQFLRSFKVEWVDEKNRGTYVVKGGVSYFENMNITIQRREVVA*

 

>CYP573A9 Aspergillus clavatus

76% to CYP573A3

ACLA_008360

MGLGESFLSQVTVTNVTLGLVAYVVLKFVYQIVYYRFFHPLSVFPGPFWGSVTRLWIAWHNLRETELPTVYALTKQYGPV

VRITPTLLLVSDPTKLPEIYHRNADKTGHYITGTFGETESLFNMRSHKTHAVFRKHVAGPYSFSNVRKMEPLIDVRIQDW

INKLEDKFAQTGKGFDFAWWAVYMAYDIISEIGFGAPFGFVEQAKDVGGLIRGFHDGLPAFGFLARLHPFTSWVKTTFLK

KYLVATPQDDSGIGILMRFRDRLIDQRFRELEANKDIGRIDLLQTFIEARTEDGSPLEIEYLKAEVLLVLLAGADTTGTV

FQALIHFLLTHPAAYRRMMKEIDSVSAQGLLSDPVPQYDEITAYLPYYVACVRETLRLNPPAPNIFPRYVSEPGLDLYGR

FAPAGTEVSGNPWIMQRNPAMYGEDAEEFRPERWLDADKAKVYNKYLFTFGYGARMCLGREIAMMELYKGPLQFFRRFNI

TPMIDKPAARFVIKGVLGSTGICG*

 

>CYP573A10 Aspergillus terreus

78% to CYP573A5

ATEG_01019.1

MGVVEEGLLSKLTVTNAVLALVAYVGLKFLYQIVYYRFFYPLSVFPGPFWGSVTRLWIAWHNMNETELPTVYELTKKY (1)

GPVVRITPTLLLVSDPKKLPEIYHRNADKTGHYITGSFGETESLFNMRSHKTHASFRKHIAGPYSFSNVR

KMEPLIDARIGDWLAKLEEKFVSSGEEFDFAWWAVYMAYDVISEVGFGAPFGFIEQGKDVGGLIQGFHDGLPAFGLLARL

HPFTSWIKTTFLKKYLVAKPEDDSGIGVLMRFRDRLIDQRLRDMEKSGGDIGRIDLLQTFLDARTEDGKPLDMEYIKAEV

LLVLLAGADTTGTVFQALMYNLFVNSEIYERMMEEIDNAVRKGLISEDMPQYDEILEHLPYFVACVRETLRLNPPAPNIF

PRYVSEPGMDLYGRFAPAGTEISGNPWIMQRDRGLFGEDAEEFKPERWLDPEQAKLFNKYLFTFGYGARVCLGRDVAMME

LFKGPLQFFRHFKPTVVPGKPPARFVIKGGVGFWRDLWVTIEKRPQVTRE*

 

>CYP573B1 Mgf Mycosphaerella graminicola

42% to CYP573A7 A. niger, 43% TO 573A2

MTARPATVRNVMSSLSTRDLAYGALFSLTWVVVVHVIWRIVHYRYYHPLRHYPGPWLASVTRMWLAWHHFWGTELHAQWALIKKHGPVIRITPTMLLVADSKEMPIIFHRRDTKSRFYLQGYVGKSNSILLREPGAHAAHRRLIGAPYALANIQRTEPLLDKHILHWISTIDARYAALGKPVDFSHWSHFLAYDTITDLGFRNPLGFVKAGSDVGNLIGGFRIGMLMFGVAGRLYPFTEKLLNSWFKKWLVVRTEQQLGFAVVMEKAGAILAERRDRIKRGLKAEKGEGSYDLLQAFMDARTPDGDHLADDTIVSEIFVVLGAGSDAFGSASTAFMASILSRPTIFRRLMDELEDAIADGKLSYPVPSYAEVSQHLPYYAACIKETLRLDPSGATLLPREVCPGDPELILNGHVVPIGTEVAMNPWIAHRDVNLYGPDAEEYNPDRWLSDPARTKIYEKYNLAWGYGARLCLGKPFAMMELYKGPLSLLLNFDVTIAEIGPNTPAPHSEMYATVKVWGDVWLQLQRSDRWSSWSSLGKSHIPGEIEDRSGQDGETEVR

 

>CYP574A1 Magnaporthe grisea MG05854.4  poor match AACU01000833 cont2.1101

MIHSTVHLGTYCLAAAAFGLASHWTYWVHGHRVRHATWTFLFHLAALFIL

GWKLTSVQGNLLSGLGATAAIFLSYLGTLFTSIVVYRVFFHRLAHFPGPF

FAKVTKLYGLYAARNSQVNDHHTSLMRQYGDFVRIAPNELMVMAPEALSK

VQGMTSKCSKKNTGIFDILHSKGDRMIVALLEKDEHIARRKIWNQALDTK

LLPQYEPKVRKEVMLWLDTLSKIQGPIDITHYMMLLSFDLTGAVGYSADY

QTIKTGKENRTLHLLEASFKPFGKLGFLAWPVQIALELGLAKEQKEFEKL

AVKTVDERVADDSEEKVDILKYFLQDFRTKQTSFRSMNSIYTESQAILIG

ATDSTSGTMAWILCYLIKHPSVAATLLQELEPVFGKTTPGEFSDADLRGL

PFLQAVIDETLRLQPPAGNGSARMTPPEGIEIAGTHIPGQVSVWVPVRAQ

QRDERFFAQPDDFIPERWTTRPELVLDRRVYAPFNTGRWSCVGKQLSFMI

MRMVIAHTLWHYEPSFAPGEDGNDIGLKRQDLIIAKPGKLELRFTPRKPR

I*

 

>CYP575A1 Magnaporthe grisea MG10343.4  poor match AACU01001708 cont2.1991

MNFSLNSLSHAQVLGLGVALCLLGYIVTTELRYRHLWGYPGPILARYTNA

YRAYLAWRYANRPGGISYHEYLHNKYGTTVRMGPNIVYTDDPDAIPLVLG

FKDRLMKTDHVNALQVPGQPANIGGIRDEKEHNRSRSAIEVVYSLSSLKG

YEPLVDETIVELTRVFEDKARAGEVVNISEWCHYYAYDAITNVTYGEPTG

FIRNGRDMFDLISSQLKHIAYVRMATQWPFMDYVLRTNPIALALNKQKAS

RAGTFFQYSADRVNAEMAKEKRSAVAQKTMLHHLIDARDKHPDVLDDQRV

QMFCTVNLLAGTLSPSFAVDFIMRWLAQNPEKQERVYREVKQHSTTYPVP

LDDTFKMPYLQGLVREGYRLSYASDIGMERVAGPKGIPLRDGRMLPPGYG

IGLSHPAIQKDAAVFGDQPEVCRPDRWMQGPDESLADYKARKGYMDRTDL

TFSKGSRNCIGKGYTQMEVAKVVASVAARFQLHLAGEAHIRDLLRIRNRL

SVILKGTD*

 

>CYP576A1 Magnaporthe grisea MG09057.4  69% to CYP576A2 AACU02000558

MAVFSVVLGVAAMLATMILIQVRGILKAYRSPLSRIPGPWYAPLTTMHLR

WGFSTGEIWKLVERSHKAYGPIVRLGPRQVWVADKDALKEILVKVDLPKV

AMYSEISRDKHSPGLFGEIRYEPHKRLKRFLSPAFTVAYIDNLEKYFQKT

MRELLNKYESQMNADPGAVSFNGIEVDLMDDLHNVALDIMGECSFGKGFG

QTNPKTSVVEEGVDEKVWKSIPRSIFDGLSKRYQMVYVKKFLRMFGLDVN

FDWPAEMIT (0)

QQQTSRRDLLQHLIEEGKKPDSGTAMNTRDIVDQMAEILLAGSETTSGTIACLFL

ELARNADVRAKLLASLPARGAGDEVVASKAIRSDAGQYWYLEACIKENLR

LHPIASEMGRRTGKDWVTIMGYDLPPHTVVSASYRDLHRNEKYWPEPLRF

WPERWLPEGQRDGAPAADMNAYYPFSAGKHSCIGINFAWAEMRMVAANIF

SRFDIEEVPGQVIDFRQYITMQFHTGNWRVVLRPRSRPAPPASSKSDDKN

QGEKQETLIEA*

 

>CYP576A2 Phaeosphaeria nodorum SN15

AAGI01000365.1 cont1.365, whole genome shotgun sequence

69% to 576A1

43011  MYIFIIYILFVAIAASQVFKRIKAFRSPLREVPGPWYAPLTTLHLQYLFSTGNIWK  42844

42843  YVEKGHKCYGPIMRVGPRQVWISDKDAMKQILVKQDLPKVAMYAEISRDRASPGLFGEI (2)  42667

42610  RHEPHRRLKRFLSPAFTVAYVDNLEVHFQKIVRDLLNKYQSKVVSSAASERGFETDFM  42437

42436  EDLHNVALDI (2)  42407

42357  MGECSFGKGFGQTNPESKGEDGVDERIWKSIPRSIFDGLSKRYQ  (0) 42226

42155  TVYIKKLIRFFVMDIKFDWPAEMIA (0)

41990  TSGTERQDLLQHLLEQGKRPDTGVTMNTRDITDQMAEILLAGSETTSGTLACLFLEL  41826

41825  ARNPVVYNKLLESLPSASLADDIVSSKTVRTESQYEYLEACIKENLRLHPIASEMGRRT  41649

41648  GTEWVNLMGYDLPPHTVVSASYRALHLNEEYWPQAQRFWPERFLAKDKRGDAPAAD (2)

       MDAYYPFSGGKHSCIGIN (2)

       FAWSEMRIVA  41289

41288  ASILSRFRLFEVSNQEVDFRQYITMQFATGEWKVILEPRQVEMS*  41154

 

>CYP576A3 Aspergillus terreus NIH2624

AAJN01000022.1 cont1.22, whole genome shotgun sequence

AC boundary before RHDPH (intron 1)

60% to 576A2, 59% to 576A1

ATEG_00955.1

65914  MGRLVEQGIVHPYYSFILLIIFLLCVSYIIGVVKAIVSPLSRIPGPWYAPLTTLHLNYAFATGKIWKIVEKG

       HQEYGSIMRLGPRQVWVSDMEAMRTILITADLPKVTMYAEISRDRSSPGLFGEI (2) 65537

65481  RHDPHKRLKRFLSPAFTVSYVDKLEPLFTECLKDLIVKYSDAFSSRRFETFTQPA

       TMDLMDDLHSLALDI (2) 65272

65224  MGECSFGRGFGYTNPQKETTFGIDEKIWSGIPSAIFKGMAQRYQ  (0) 65093

65040  MVYFKRLLRKIGVDIKFDWPDSMIA (0)  64960

64856  KNLGPDLLQHLIDEGEKPDSGMKMVPRDVIDQMSEVLLAGSETTSGTIGCFFLEVLRNPQVKARL  64677

64676  LEALPVLLPNDSIITSKAVRTDSQYEYLEACIKETLRLHPIASEMGRRTLNQSIELIGFS  64497

64496  IPPHTFVCASYRDLHRNPAYWPDPLRFWPERWLQNRPPDVP  64374

       LTAYYPFSAGKHSCIGKN (2) 64250

64153  FAWAEIRMVTANLLSRFEFIEVPAQDIDYRQYITMQFANGSWKA FLKPRYESRAEFFTI* 63974

 

>CYP576A4 Aspergillus niger

e_gw1.20.142.1|Aspni1

68% to CYP576A3

METISERLPVYFHWAPLFILALLFFNYIIGVTKAIKSPLSRIPGPWYAPLTTLHLNYAFAKGTIWKAVERSHAKYGPIFR

LGPRQVWISDKEAIKAILMTVDLPKVTMYAEISRDRSSPGLFGEIRPDPHKRLKKFLSPAFTIAYVDGLEFLFSECVGDL

INRYVDLLSSPTSRGQKELVVTDLMQDLHSLALDIMGECSFGNGFGQTNKNKKLELELDEGIWRSIPTAIFKGMTRRYQF

VYIKRLLRRLGLNIEFDWPREMIAAISAVAGHRKAKPKSVRPDLLQHLLENGERPDSGVKMGTREVVDQMAEILLAGSET

TSGTIACFFLEILRNPEIKEKLLKSLPVLRPSDPIISSKTVRTSPEYEYLEACIKEVLRLHPIASEMGRRTGNTPVKLMG

YYLPAHTIVSASYRQLHRDPKYWVDPLRFWPERWLDPRPSDVPAPDMHAYYPFSAGKHSCIGKNFAMAEIRMLTANILSR

FDLDEVPGQNIDYRQYITMQFEHGSWKAFLTPRYEQSTPVS

 

>CYP576B1 Mgr021 Mycosphaerella graminicola

52% to 576A1

MGIISILLPSLAAFLLFQWAISAWRAYNSPLSALPGPWYAPFTNIHLQIGFARGTVWRQVEAAHAKYGGMMRLGPRQIWV

SDKVALKQIISQIDLNKVMMYAEMSRDKNSAGLFGEVHKRLRKLMTPAFTVGYLDQMDHLFQKPVEDMMNLYKSNLKPKS

AVDGMKVNLMTDLHKIALEVIGECAFGRGFGSVAPDTEPEPGFSKEQWQKIPDNIAKGLSMRYAIVFLKRSLRKLGIHME

FDWPAEMVAAIESVITRRRAGHEAGRQDLVQHLIDNGVRPDNGQKMSARDILDQLSELMLAGAETTSATMCYFMMELARN

PDVRKKLFKDLPALGLDDPLITGVEVRQDPRFAYLTACMQENLRMHPIASEFGRRTTDKPVVLNGFEVPPYTVVSASYRA

LHYNEEYWPQPHRFWPERFLPIDHPLKGDAPNADTSAYYPFSSGKHSCVGMNFAWHEMRVVLANYCARFDITEEPGQKED

IRQYITMQFHDGNWNARLYPRL

 

>CYP577A1 Magnaporthe grisea revise and shorten at C-term

MNQGNLIYTATQYCAPHLAGIATTLAIAYVLYALIVLPLAASPLRAIPGP

KAYAATRWRLAWDDYRGARTRVIHSLHKQHGSAVRIGPGEVSFSSLSALR

TIYGAGSGFERTNFYRMFDVYGRQNLFTFAGAKKHGERKKVLAHAYAKSV

VLADGSVAKPLIENNVRRFLELIEREKEATSKIFDSLHWFSLDSITGFLY

GDQHGGTRALRGDRADRAMLDDIRDPARRKLTWFAFHLKLYTKWLYSQTG

LLEKTINVLGLLPMSKPTTYTAIRAHALKSCVASEAETNETNVTVDKASF

IMSKLWAYSKSEKEPRMDRLDIASEAADHFLAGIDTTSDTLMFAIWALSL

PENRRCQERLIAEIDSMSPELFNQGTKALGSAVAAAEVADRLPYLNAVIK

ETLRLFAPIPGSEPRSMPVDTTIDGYRIPAGTVVSMSPYTLHRNEDVFPQ

TLEFRPERWLGELGDLVEMKKYFWAFSSGGRMCIGVH (2) 7915

7820 FAMAEMTTLLASLYKHYSTCEQERQKGSSPAISSRFELFYDERFTQIS  (0)7674

EHACYIDFKRRTH*

 

>CYP577A2 Fusarium graminearum AACM01000316.1

Gibberella zeae PH-1 revised (formerly 577B1)

MAILPLAAAVVAVALLVKYLLDPFLSPLRQVPGPKLFAATRWRLAYEDWK

GTRTRTINALHKKYGPVVRIGPNEISFNSLSALRTIYGPGSRFGRTSFYR

MFDVYGEQNLFTFHSPKDHGDRKKLLSHAYSKTAVLKPATARMIERQAWR

YLNLIDSEPEGVSEIFSTLHYYSLDNITAFVYGKYGATAAVRGSKIHRDL

ISDILHPSRRRLSWCIVHVKGFTQWLYRQSSFMGALVKPILPMQQPTTYT

GIRAYALSAFKQFRAEADSQEVKFTEDEHVSILERLWQYHETQRPDGMRD

LQMASECADHFLAGIDTTSDTLMFLVWSLSLPGNEKFQEKLREEVQAISD

DSLNKQGIPKAEVADRCVYLQAVIKETLRLYAPLPSTEPRSTGDDVTIDG

YVIPKNTVVGMSPWIMHRNEEVFENPLVFNPERWLGEKASELNRWFWGFS

SGGRMCIGMHLAMAEMTVLGAALYREYRTTIAPGFEDTSPAITARVETFY

DERFPKVK (0)

ESTCLVKFDKLKK*

 

>CYP577A3 Nectria haematococca

estExt_Genewise1.C_270019

Necha1/scaffold_27:156564-158470

64% to 577A2, 55% to AP007157.1g orth Aspergillus oryzae

last exon seems unneeded, continue exon 3 to stop codon eliminate exon 4

MASFSITIALSVLFLLLIKYYVLDPLVFTPLSRVPGPTAFALSKWRLGYEDWKGTRTPTIQHLHSKYGPA

VRIGPNEVSFNSLTALKTIYGPGSRYGRTAFYRMFDVYGEQNLFSFHSVEEHGSRKKLLSHAYAKSTVLK

APTTQLFEEKTRQYMELISGEPNHISDVFHTLHYYSLDNITSFIYGKHGSTSALQGSEADRALISDIMHP

SRRMLSWCLVHMPSITKWMYARTGFMRQLMKPLLPMQLPTTYTGIRKFALQAFENFQSDSEKTN (1)

GNERSILAHLWQHHQTQKTNGLNDMQTASECADHFLAGIDTTSDTLMFLLWSLSLPENQRFQDKLRDELLGLPAEA

LNQHGYPKAEESDRCTYLHAIIKETLRLYAPLPSSEPRSVNAASVVDGYHIPADTVVSISPWILHRNPKV

FKDPLEFNPERWLGPDATELNRWFWAFSSGGRMCIGMH (2)

LAMAEMTTLIATLYREYQTSIAPGFENTTPGITARVEVFYDERFPKLQ (0)

ENTCLIKFDRLRK*

 

>CYP577A4 Aspergillus oryzae RIB40

AP007157.1g orth genomic DNA, SC023

53% to 577A2 probable ortholog

join(1148972..1150341,1150393..1150552)

1148972  MTITLVAIATGIVAFILYYICWSLFSSPLARIPGPKSFALTKWRLAYEDYKGTRTRKIHALHERY  1149166

1149167  GPVVRIGPNEVAFNSLSALRAIYGAGSGFERTNFYSMFAIYGRKNMFSFNTVKQHAERKK  1149346

1149347  LFAHAYAKSAMLKGINASMIETKVRKYMEFLEREGRSQDIFTTLHYFSLDNITEFLYGD  1149523

1149524  FGKTACLDGIEEDRALLHDILATGSRTLSWLTVHHPKLTAWLYSRTGFLGCVARQFYPMQ  1149703

1149704  RPTPYTSIKLHATKAFQGFADASVAEKAKQSSLISKLWKHHRSMKDGGLDNLDIASECAD  1149883

1149884  QLDGGIDTTSDTLMFTIWSLSRPEHWGFQQKLIDEVRSISEHDLNPEGIPRVEAADKLPY  1150063

1150064  VEAVIKETLRLFAPLPASEPRSSPEATTIDGYLIPARTVVSISPYTLHRNAEVFKNPLEF  1150243

1150244  NPDRWLDPSQDLTEMNRFFWAFSSGGRMCIGMH (2)

1150394  LAMAEMTTFVAALYRKYTTEPRGGFGTISPGITSRFEVFYDEGCSDMR (0)  1150537

         EHQCQIEFKLQ*

 

>CYP577A4 Aspergillus flavus

99% to CYP577A4 Aspergillus oryzae

AFL2G_04292

MTITLVAIATGIVAFILYYICWSLFSSPLARIPGPKSFALTKWRLAYEDYKGTRTRKIHALHERYGPVVRIGPNEVAFNS

LPALRAIYGAGSGFERTNFYSMFAIYGRKNMFSFNTVKQHAERKKLFAHAYAKSAMLKGINASMIETKVRKYMELLEREG

RSQDIFTTLHYFSLDNITEFLYGDFGKTACLDGIEEDRALLHDILATGSRTLSWFTVHHPKLTAWLYSRTGFLGCVARQF

YPMQRPTPYTSIKLHATKAFQGFADASVAEKAKQSSLISKLWKHHRSMKDGGLDDLDIASECADQLDGGIDTTSDTLMFT

IWSLSRPEHWGFQQKLIDEVRSISEHDLNPEGIPRVEAADKLPYVEAVIKETLRLFAPLPASEPRSSPEATTIDGYLIPA

RTVVSISPYTLHRNAEVFKNPLEFNPDRWLDPSQDLTEMNRFFWAFSSGGRMCIGMHLAMAEMTTFVAALYRKYTTEPRG

GFGMISPGITSRFEVFYDEGCSDMRERLQG*

 

>CYP577A5 Gibberella moniliformis

AAIM01003330 GC boundary at CVGM

2400 MAILPIAAAVLAVVLFIKHIFLDPLILSPLRHVPGPKSFAATKWRLAYEDWKGTRTRTIF 2579

2580 KLHQLYGPVVRIGPHEVSFNSLSALRTIYGPGSRYGRTTFYRMFDVYGEQNLFTFHSPKE 2759

2760 HGDRKKLLSHAYSKSVVLKPPTAKMVERRVRQYLDLIEAEPENISEIFSTLHYYSLDNIT 2939

2940 AFVYGKYGQTAAIRGSKIHRELIADILHPSRRKLSWSIVHLKTLTQWLYRQSGLMGQLVK 3119

3120 PVLPMQQPTTYTGIRGYALRAFEAFRADVEKITHSE (1)

     DEHVS 3293

3294 IVENLWQHHESQKPGGLRDLQMASECADHFLAGIDTTSDTLMFLVWALSLPGNEKYQEKL 3473

3474 REEVMAISGDGLNQWGNPRAEASDRCTYINAVIKETLRLYAPLPSTEPRSIDIDSVIDGY 3653

3654 SIPGNTVVGMSPWIMHRNEKVFKDPLVFNPDRWLGPDAAELNRWFWGFSSGGRMCVGM  (2)3827

     LAMAEMTTLTATMYRQFRTTIAPGFEDTTPAITARVETFYDDRFPK (0)

     ESKCLIKFTKLKE*

 

>CYP577A6 Mgr082 Mycosphaerella graminicola

55% to CYP577A3   Nectria haematococca

MLTLIIFAASLAAAFYQFIVQPLYLDRLSKIPGPKLYALTKWRLALEEWRGARTRTIDALHNQYGPVIRIGPNEIHCNSLAALRTIYGAGSGYGRTSFYRMFDAYGKQNLFTFYSNKAHGDRKKLVANAYSKSVILKGPTASLVREKVSQYMSLIEEGGTKPHEIFSSLHYYSIDSITHFLYGSKFGGTSALTGSTADRKLLQDILDPTRRRLSWFAVHLPALTKWLYTRTGSMERLVKPLLPMQKPATYSGIRKHALDAYWGFEKAATAEPSATLADPTIITQLWKSHERVKASGAGALDSLDIASECADHLLAGIDTTADSLMFLVWAVSRPQHEALQRRLIDELRSLPEDELEDGVPKLETLDTLPVLNAVIKETLRLYSPLPASEPRSLGVDRTVDGYDIPAGTTISMSPYSLHRNEEVFKDPLTFNPDRWLNSSLEEVAEMNRWFWAFSSGGRMCIGLHLAMAEMTALVAAVYREYTTSIAPGFENKTPAITSRFEMFYDVTVPEIAEHECMITFERH

 

>CYP577A7 Fusarium oxysporum

97% to CYP577A5 Gibberella moniliformis

FOXG_13528

MAILPIAAAVVAVVLFIKHIFLDPLILSPLRHVPGPKSFAATKWRLAYEDWKGTRTRTIFKLHQQYGPVVRIGPHEVSFN

SLSALRTIYGPGSRYGRTTFYRMFDVYGEQNLFTFHSPKEHGDRKKLLSHAYSKSVVLKPPTAKMVERRVRQYLDLIEAE

PENVSEIFSTLHYYSLDNITAFVYGKYGQTAAIRGSKIHRELIADILHPSRRKLSWSIVHLKALTQWLYRQSGLMGKLVK

PVLPMQQPTTYTGIRGYALRAFEAFRADVEKITHSEDEHVSIVENLWQHHESQKPGGLRDLQMASECADHFLAGIDTTSD

TLMFLVWALSLPGNEKYQEKLREEVMAISGDGLNQWGNPRAEASDRCTYINAVIKETLRLYAPLPSTEPRSIDIDSVIDG

YTIPGNTVVGMSPWIMHRNESVFKDPLVFNPDRWLGPDAAELNRWFWGFSSGGRMCIGMHLAMAEMTTLTATMYRQFRTT

IAPGFEDTTPAITARVETFYDDRFPNVQESKCLIKFTKLKD*

 

>CYP577A7 Fusarium verticillioides

98% to CYP577A7 Fusarium oxysporum

FVEG_13880

MAILPIAAAVLAVVLFIKHIFLDPLILSPLRHVPGPKSFAATKWRLAYEDW

KGTRTRTIFKLHQLYGPVVRIGPHEVSFNSLSALRTIYGPGSRYGRTTF

YRMFDVYGEQNLFTFHSPKEHGDRKKLLSHAYSKSVVLKPPTAKMVERR

VRQYLDLIEAEPENISEIFSTLHYYSLDNITAFVYGKYGQTAAIRGSKIHR

ELIADILHPSRRKLSWSIVHLKTLTQWLYRQSGLMGQLVKPVLPMQQPTTY

TGIRGYALRAFEAFRADVEKITHSEDEHVSIVENLWQHHESQKPGGLRD

LQMASECADHFLAGIDTTSDTLMFLVWALSLPGNEKYQEKLREEVMAISG

DGLNQWGNPRAEASDRCTYINAVIKETLRLYAPLPSTEPRSIDIDSVIDG

YSIPGNTVVGMSPWIMHRNEKVFKDPLVFNPDRWLGPDAAELNRWFWGFS

SGGRMCVGMHLAMAEMTTLTATMYRQFRTTIAPGFEDTTPAITARVETFY

DDRFPKVQESKCLIKFTKLKE*

 

>CYP577A8 Mycosphaerella fijiensis

59% to CYP577A6   Mgr082 Mycosphaerella graminicola

e_gw1.6.418.1

MLEILVAATALLIAAYSIILEPLSLNPLSKIPGPKLYAITKWRLAWEDWQARRTRTIHALHQKHGPVVRTGPNEVHFNSL

TALRTIYGPGSGFGRASFYRMFDVYGKQNLFSFHSAKDHGERKKLLANAYSKSSVIRGHVAEMVSEKIQDYIHLLETQEN

IFTSLHYYSLDSITHFLYGTHGATQALKGDPASRSLMTDILDGPGRMRLTWFAVHLPAFTKWLYSRVGAVERLVRLFLPM

QKPLTYTGIRAHALKAMHMYTAAAAADKIDDTAKNTIIGRLYQSNAKLSDLEIASECADHFLAGIDTTSDTLMFLIWALS

RPGNEGIQQKIIDEAR SVPESDLDQYGAPTVEAADKLRYLDAVIKETLRLYAPLPASEPRCADIEMIIDGYTIPAWTTVC

MSPYSLHRNEEVFPEPLKWSPDRWHGDTEHVSMMKKWWWAFSSGGRICIGSNLALAEMALVPAIYRRYRTKIKSGEEKAS

PGITSRVEVLWDETFAEMKVSILFRGCV*

 

>CYP577A8 Aspergillus clavatus

56% TO CYP577A2 Fusarium graminearum

ACLA_067130

MESYMVKSFLLGVSLSLLYLIGKVFINLFQSPLRSIPGPKLFAITKWRLAYEDYRGTRTRYMKTLHDRYGDAVRIGPNEV

SFNSLSALRTIYGAGTVFQRDTFYRMFDAYGKQVMFSFASSKDHRARKKLLNHAYSKTSVLSPNNASMIEEKARQFMDLI

EKEAKDGSLEIFAALHYFSIDAITKFLYGKSAGATTALTASQDRSLLDDILDPDRRKLSWFTIHFPTLTNWLYSRTGLLE

SLLTDLGLLPMRKPSTYTGIRAHALAAAEHLSQSTTESLDVNASIAERLLVAGRTADPKGVPMDHLDVASECADHLLAGI

DTTSDTSMWAMFALSQPENQIFQDKLRAEVRSLGEEVTTDRVVSAIAADKQPYLDAVIKETLRLFAPLPGTEPRFADTDQ

IIDGYSIPKGTVVSMSPYTLHRNARVFQDPTRFNPDRWLGDAEAVAEMKRWFWAFSSGGRMCIGMHLAMAEMTTLLASVY

RTYKTEITPGFEGVSPGVTARYEVFSDEQFPRMEEHVCWVKFVKE*

 

>CYP577A9 Aspergillus terreus

54% to CYP577A8

ATEG_04675.1

MIPILCLGALLFGLVLFHVHKNLISSPLRHLPGPKSFAFTKWRLGYEEYRGMRTQCVNNLHKKYGEVVRVSPYEVSFSSL

SALRKIYGSG SRFERDDFYQMFDAYDRKVLFSYSSGIDH RERKKILNSAYTKTAIMSPSNVDMIEEKVKDFLALVQRDSS

ATGAVEIFSALHYFAMDSVSHFVYGATSPGRTTAMKGTRQHRGIIDDMLDPARRKLFWYAVFWPRLTEMLYAQGAIVQKS

LASFGLLPMKTPTYTGLRKHNFPVIQHLETQQLLGSDDSLASRLLAGTIPSAKASAPMDTLDVAAECSDHFLAGIDTTSN

TLMWVIYQLSRPENEGFQSKLRAEIQALDGPAGSGTFIYPEKAGTLPYLDAVIRETLRLHAPSPGSEPRWSTNDEVIDGY

TIPRGTVVSISPYTLHRQPQVFANPLRFDPERWLGPEDQVAERKRWFWAFSSGARMCIGVHLAMAEMTTLLANIYQNYWT

HIPSEYLQVSPGMSARGDLFYDEHLSQVEEHKCWIQFVPIRALSTCLQGCV*

 

>CYP577A10 Aspergillus terreus

70% to CYP577A8, 56% to CYP577A9

ATEG_07313.1 this model is a fusion of two genes

MATFSLLTQYIWKWMQKSLFL SIFVAAIALVVLYGLAKVHRNLIVSPLRPVPGPKFFALTRWRLAYEDYRGTRTRCINNLHKQYGDVVRVGP

NELSFNSLSALKSIYGAGSGFQRDKFYRMFDAYGRQVMFSFAFSVDHR (1)

ERKKLLNHAYSKTAVLSSRNAAMVEEKTRQFLSLLDREAKSGIMEIFNALHYFSMDSITQFL

YGESQGATTALTASANRSLLDDIIDPARRKLSWFAVHFPHLTHWLYSRTGAMEWLLTTFGLLPMSKPSTYTGIRRHALVA

AEHIRDQTIEDPTESIAARLFHAMRTREKGPAMDYLDVASECADHFLAGIDTTSDTLMFAIFALSQPANLSFQEKLHQEI

QSLPDEVVVDDVVNALAADKLPYLDAVIKETLRLYAPLPGSEPRWSEKDEVIDGYRVPARTIVSMSPYCLHRNEHVYKEP

LEFNPDRWLGPSEQVAEMKKWFWAFSSGGRMCIGMQ (2)

LAMAEMTTLLASVYRKCRTEIAPGFERVSPGVTARYEVFYDEKFPRMEEHICWIRFRSR*

 

>CYP578A1 Magnaporthe grisea MG04605.4  poor match AACU01001135 cont2.866

MPFPVASSLLLSLVSIPVLVLTYHVIIYPAFISPLRKIPPAHWTVPFSPL

WILIVRLQGRENRVLHLAHQKLGRFVRVGPNELSVNDAGAVRTIYQGGFE

KTAWYSIFDNYGVPCMFSTVPAKEHSLRKRMISNVYSKSYIQSSQAAKAQ

AAAILHARLLPILEESAAKGREPHGIDIHSLFLAAAMDLITAYIFGLRHS

TDFLRNRGYREHWLQLYKARANYPFWRQELPRFTEVMSRLGLNLYPSWVD

ECNRELGEWNKKLCITVLASGVIGEETKPVDEPVVTKAMLAGLDKEAQAN

GDKSILYSTALQHHDLSVASELFDHILAGQETTGITLTYLVWELSRDQAL

QRKLQSELQTLDPGLKKQRDETGQWTATDAGSIPDLKALDSLELLHAVLM

ETLRLHAAIPGPQPRQAPHPSCRIGPYDVPGGTRVSALAHTLHREEAAFH

EPEKWDHERWLGGDEEARKLMNRHFWAFGSGGRMCIGSNFAMNEMKLIIA

AIYSNYTTHIVDDAGIEQDDGYTAGPKSGRLWVRLEPLV*

 

>CYP578A2 Nectria haematococca

e_gw1.24.56.1

Necha1/scaffold_24:249201-250960

53% to 578A1 in a P450 gene cluster

MGLVIPTAIGALALLLLYHHVLHPAFISPLRKLPAAHWTCHFSSIWILAARLYRRENRSLHEAHITLGPV

VRIGPAEVSVDGVEGMRVIYQGGFEKGFWYSVFSNYG (2)

VPNMFSTGSSKHHSARKRMVANVYSKSYLQSSQ

ASKAQMSHIVFQRLLPALSEHSSKDKLVS (1)

IFGLFLALAMDVITAYLFGLSNGTDFIQDEQYRQSWQEMYL

ARANYPFWTQEVPNLTAACV RWLPWLRLYPKWVDESNAKLCQWNLNLCQG (0)

QGKASAEPCEEAVVFNALQYGIDRELRTNGEKSILYRTSICERELA

VASELMDHSLAGHETTGMVLTYATWHLSKSPDLQEQLRSEIISIGSSLKLGRPSTVEAHEFEDTKSGPSL

PDLKALDALPLLNAIVMETLRLHAPIPGPQPRDTPKEGCNIAGYHIPGGVRIASMAYSLHRDPKVFPQPE

SWKPERWLPQDGTDMETSHREMHRQFWAFGSGGRMCVGSNFALNEMKVILAAIYANFRTTIVNDDGIEQE

DAYTARPVGEQLVLRFYTLDA*

 

>CYP578A3 Fusarium oxysporum

64% to CYP578A1

FOXG_06037

MGLILVTALGLLPVLLIYRYIIYPVFFSALSRIPAPHWTCHISPLWILAARKWRRENTSLYRAHQRLGPVIRIGPNEVSV

DGLEGMRTIYQGGFEKGHWYSVFDNYGVPNMFATGNSKHHSLRKRMISNVYSKSYIQSSQVSKLQMAEILVNRLLPALDG

SLEYSRKSISTDYNDVEVFGLYLATAMDLITAYIFGLSNATNFIADEPYRRDWQDMYLARANYPFWTQEVPKLTNFCKRW

IPWLKLYPQWVDQSNKKLSDWNWELCENVRKTSKVKRSPGNSQDIKFSDEPVVYNSLLAGIDREFKTNGEKSILYSTSIL

QRDRAIASEVMDHSLAGQETAGIALTYATWHISKSPELQRQLHAEVQSLKPSLMTDPKAASVITNTSALPDPKEVDSLPL

LHAIVTETLRLHAPIPGPQPRQTPKNGCSIGGYYIPGDVRIASLAYTLHRDKEVFPEPGEWNPERWVEEKTRKSPEDEVK

HREMQRVFWAFGSGGRMCVGSNFAMNEMKFILAFIYANFETSIVDDEGIEQEDAYTARPIGEKLVIRFTPLKT*

 

>CYP578A3 Fusarium verticillioides

92% to CYP578A3 Fusarium oxysporum = ortholog

FVEG_03904

MGLILVTALGLLPVFLIHRYIIYPVFFSALSRLPAPHWTCHISPLWILAARK

RRRENTSLYRAHQRLGPVIRIGPDEVSVDGLEGMRTIYQGGFEKGHWY

SVFDNYGVPNMFATGNSKSHSLRKRMISNVYSKSYIQSSQASKLQMAEILVSR

LLPALDGSLKSSHKSISTDYNDVEVFGLYLATAMDLITAYIFGLSNA

TNFISDEPYRRDWQDMYLARANYPFWTQEVPKLTSFCKRWIPWLKLYPQWVDQS

NKKLSDWNWELCENVRKTSKATRSPSSSQDIQRSDEPVVYNSLLAG

IDREFKTNGEKSILYTTSILQRDRAIASEVMDHSLAGQETAGIALTYATWHIS

KSPELQRQLHAEVQLLKPSLVINPKAASGLTNTSALPDPKEVDSLPL

LHAIVTETLRLHAPIPGPQPRQTPEHGCNIAGYYIPGDVRIASLAYTLHRDKE

VFPEPEEWKPERWISPTPGKSPDYEGKHREMQRLFWAFGSGGRMCVG

SNFAMNEMKFILAFIYANFETSIVDDEGIEQEDAYTARPIGEKLVIRFTPLKK*

 

>CYP578B1 Aspergillus nidulans AACD01000016.1 revised

MFTHTAIFTLSFLLTVSFLLFIYLIHPLFLSRLSKLNIPNAHFTSPLSSY

WINSIRHAGLETVTIQALHAKHGPVVRLSPNELSVNSLHGLRVIYTGAFE

KHALYRDLFLNFHTENLVGMLGNKEHARQKRMLSRVYSKSYLQESEDMRV

ISAVILSTRLLPILQRIAENGETVNVLPLFQAVGMDFTSAYLLGVQNGTS

FLFDLPGWQRWLEEYEKFKHLSLNERADGFIERWCLDLCRQTQTESSSSD

AISTKPVVYNALRHGLEKSPDSRPSDLAIASELLDHLIAGHETSGITFTY

MMWELSHCPNLQDELRAELLLLQPSLKYPFPSGGGNGSFPHPSSVDNLPL

LDAIVRETLRLHAPASSPLPRVTPDTPGGTSIDGFDGIPGGITVSSSAYT

LHRIEEVYPQPTEWLPQRWLDPGSGKKHDMRRLWWPFGSGGRMCLGSNFALQ (1)

EIKLVTAAVYTNYTTAVVDDEGIEQDLADFISLPKGRKLVLRFDPINRA*

 

>CYP578B2  Aspergillus fumigatus Af293

          GenEMBL XP_748713.1 also EAL86675.1

          65% to 578B1

MFLLTIVAVSSFLVLLLYKILISPYFLSPLSKIPNAHFTAPISDYWIERKRRSGEEVLTIYNLHREHGPV

VRLGPNELSVNSLGGLHVIYTGAFEKHSFYRDTFVNFLTDNLVGMLPNDSHARQKRMLSKIYSKSYLHES

VDLRNASSIILSQRLLPVLKNAAEKGEAINVLPLFQAVGMDFTSAFLFGTANSTTFLFDLPGWKRWLVEY

ERFKTLSGKERYLGFIERWCLSLCSRVQSNEHPNDVPVATNPVVYSQLRQSLEKHPDQRPLDLALASELL

DHLVAGHETSGITFVYMMWELSKRPRLQAELRQELLTLSPSLRYPLVELGDGSLPLLPSPAAIDSLPLLD

AVVRETLRVHSPAPAPLPRITPSSSEGIAIEGYDRIPGGVKVSSSSYTLHRISEVYPQPFEWLPERWLDP

GPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVMAAVYSNYTTSIVDDEGIEQDYAFISLPKGRKLML

KFTPVKL

 

>CYP578B2  Neosartorya fischeri NRRL 181

          DS027697, NFIA_006220

          join(1665966..1667316,1667397..1667539)

          94% to CYP578B2 Aspergillus fumigatus = ortholog, 66% to 578B1

          name revised from CYP578B4

MFLLTIVAVSSFLVLLLYKFLICPYFLSPLSKIPNAHFTAPISD

YWIERKRRSGEEVLTIYDLHREHGPVVRLGPNELSVNSLGGLHVIYTGAFEKHSFYRD

AFVNFLTDNLVGMLPNGSHARQKRMLSKIYSKSYLHESVDLRNISSIILSQRLLPILK

RVAEKGEAINVLPLFQAVGMDFTSAFLFGTANSTTFLFDLPGWERWLVEYERFKYLSV

KERYLGFIESWCLSLCSRMQSNEHPNDVPVATNPVVYSQLRQSLEKHPDQRPLDLALA

SELLDHLVAGHETSGITFVYMMWELSKRPQLQAKLRQELLTLSPSLRYPFVELSNDSL

PLLPSPAAIDSLPLLDAIVRETLRVHSPAPAPLPRVTPSSPEGIAIEGYDRIPGGVKV

SSSSYTLHRISGVYPQPFEWLPERWLDPGPGKIHDMRRLFWPFGSGGRMCLGSNFALQ

EIKLVMAAVYTNYTTSIVDDEGIEQDYAFISLPKGRKLMLRFTPVKL

 

>CYP578B3  Aspergillus oryzae

          GenEMBL BAE66519.1

          62% to 578B1

MTSATAIAIGILLAAFVYKYLVHPYCLSPISSVPNAHFTSPISNRWIEKQRNAGKEVLTIYNLHQKLGPV

VRLGPDELSVNSLSGLKTIYTGAFEKHSFYSDVFINFQTPNLVGMIHNNPHARQKRMLSRIYSKSYLQES

RDLRDISKIILSQRLFPILRRVAKSGEAINVLPLFQAVGMDFTSSYLFGTKNSTTYIFHLPEWQQWLEEY

EKFKYMSVQDRYMGFIESWCLSLCRKVENNDQPNDVPIATNAVVYNQLRQSLEKDPDSRPLELAIASEVL

DHLVAGHETSGITFTYMMWELSQHPELQAELRRELLTLTPNLRSLPISDNAVDLPSLPSPSAIDALPLLD

AVLRETLRLHSPAPAPLPRVTPASPTGVSIEGYHNLPAGVRVSSSSYSLHRIEEVYPQSSDWLPERWLKP

EPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVMAAVYTNFTTSIVDDEGIEQDHAFISLPTGRKLML

RFTPIKEA

 

>CYP578B3  Aspergillus flavus

100% to CYP578B3  Aspergillus oryzae

AFL2G_11839

MTSATAIAIGILLAAFVYKYLVHPYCLSPISSVPNAHFTSPISNRWIEKQRNAGKEVLTIYNLHQKLGPV

VRLGPDELSVNSLSGLKTIYTGAFEKHSFYSDVFINFQTPNLVGMIHNNPHARQKRMLSRIYSKSYLQES

RDLRDISKIILSQRLFPILRRVAKSGEAINVLPLFQAVGMDFTSSYLFGTKNSTTYIFHLPEWQQWLEEY

EKFKYMSVQDRYMGFIESWCLSLCRKVENNDQPNDVPIATNAVVYNQLRQSLEKDPDSRPLELAIASEVL

DHLVAGHETSGITFTYMMWELSQHPELQAELRRELLTLTPNLRSLPISDNAVDLPSLPSPSAIDALPLLD

AVLRETLRLHSPAPAPLPRVTPASPTGVSIEGYHNLPAGVRVSSSSYSLHRIEEVYPQSSDWLPERWLKP

EPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVMAAVYTNFTTSIVDDEGIEQDHAFISLPTGRKLML

RFTPIKEA

 

>CYP578B5 Aspergillus niger

e_gw1.3.1489.1|Aspni1

56% to CYP578B3

MLSILLLAILPIIYLIHKYFTDPLSRIPPAHPLSPYTTKWITHHRDRTTEVKAIYTAHRARGPIIRLSPTELNVNSLEGL

RAIYTQGAFEKHAFYADTFMNFHTPNMVGMLENKPHGEQKRLLSGVYAKSYLLRSSDVKVLGREMVVGRLGEFLRGVVAK

GAVINVLPVFQAVGMDFTSGFVFGMQNGTRFLEDLTSWGVWLGQYEAFKSMSREERFMGFVERWCLGLCEGVGLSKQSGG

PVTQAVVYDRLREGLEEKKKVSGEDVDVNLAVASEVLDHLVAGHETTGITITYIMWELSKRPELQTRLRKELASLPGSLR

YTEGMEKGLPSLADIDALPFLDAIVRETLRLHAPAPAPLPRVTPPEGTWIHGYYIPGGVQVSSSAYSLHRIEEVFPSPEE

WIPWRWMDVKDDKIHDMRRLFYPFGTGGRMCLGSNFALLEIKLVIAMVYANFAPEVVDDEGIGQHLEFIALPKGRKLMVR

FVPV

 

>CYP578B6 Aspergillus clavatus

75% to CYP578B2

ACLA_060810

MSHFHLAVIAAGSLSALLLLFYKLLLSPYFLSPLSKIPNAHFTAPISSAWINRKRRHGQEVLAIYTLHRKHGPIVRLGPR

ELSVNSLAGLRTIYTGAFEKHAFYRDAFVNFFTENLVGMLGNRAHARQKRMLSKIYSKSYLQDLGDLRGLAAVVLARRLL

PVLRGCAVTRAAVNVLPLFQAVGMDFTSAYLFGTANGTVYVSDLSAWGKWLAEYERFKNATLRERYMGFIETWCLAMCRR

MQRNDHPNDVPVATRGVVYEQLRSSLEKDPDARPLELAVASELLDHLVAGHETSGITFTYMMWELSQRPRLQAELRRELL

TLTPSLQDPLVATSEGPVPALPSPAFIDALPLLDAVVRETLRLHSPAPAPLPRVTPCCPGGTTIEGYPHIPGGIKVSSSA

YTLHRIGEVYPQPLEWLPKRWLSPGPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVMAAVYTNYSTSIVDDEGIEQD

HAFISLPKGRKLMLKFTPVEIRVE*

 

>CYP578B7 Aspergillus terreus

70% to 578B3

ATEG_07890.1

MAFPSLFTGAALTLLALLYKYVVYPYFVSPLAKIPNAHWTSPISARWIERRRAAGTEVLTIYNLHQQHGPVVRLGPNELA

VNSIQGLKTIYTGAFEKHPWYGDFFINFHTDNLVGMTHNQPHARQKRMLSKIYSKSFLQESTDFNAISRLILVERLLPIL

HRTARSGTAINVLPLFQAVGMDFTSAYLFGTKHGTTYVLQPEEWAHWLEEYEKFKYLSRQDRYGGFIETWCLHLCDKTER

NDTPNCVSAETTAVVYNQLYSSLVKSPDERPLRMAVASELLDHLVAGHETTGITVTYIMWELSQRPDLQKQLYQELSGLG

RCLEYSEDGEEIFRLPSATSIDPLPILDAIVRETLRLHAPAPAPLPRVTPMSATGTTIERYKSIPGGVRISSSAYSLHRI

PEVYPHPFEWLPERWLKPGPGKIHDMRRLFWPFGSGGRMCLGSNFALQEIKLVIAVVYTNYTTSILDDEGIEQDHAFISL

PKGRKLMLKLSPVKAE*

 

>CYP578C1 Aspergillus nidulans AACD01000007.1 revised 7/18/07

MEVPVFAWIVPIMLGLTLLYEFVIYPAFLSPLSKLPNAHWTAPISPAWILWKRFRGQNNRT

IHAAHERLGSIVRLAPSEISINCVDGGIKSVYTGGFDKHEWYPRVFGSFG (2)

TVSMFTMTGSKAHSTRKRMLSNIYSKSYLQSSRHLQLISKTIIFDRLLPILQAAAS

SKTDTDVHDLNQGLTMDFVSAYLFGLANGTNFLQNAEYRREMLRLYQCRK

PYEFYHQEVPNILALLKKIGIRLIPKWCDDANRILDSWGLELCDKAEASV

ASSDLSVEPVVYKHLKQAISKQAPLKIDDQKLYADYLEQQRLDIACEMYD

HLTAGHETSAVVLTYLMWELSQHIEVQKDLHKELLTLQPRIAPRCLSGHA

ELPSPKSIDSLPFLEAILTETLRLHASIPGIQPRVTP

TPTCTLVGYDNIPPNTRVNAQAYSLHRNPEVFPDPETWEPKRWL

KDCNKPSELEERKRWFWAFGSGGRMCVGSNLALQ (1)

KMKLVIAAIYTNFRTTIVNDDGIEAIDAYTVKPKGEKLILRFEYI*

 

>CYP578C2 AP007155 Aspergillus oryzae RIB40

71% to 578C1

MAGSPIVAISAIVLTITLLYKCIVYPVFLSPLSKIPNAHWTAPISPAWMLWKRFRSQNNR

TIQAAHERLGPIVRLSPSEISINCVEGGIKTVYTGGFEKHEWYPRVFGSLG (2)

TVSMFTMISSKPHSVRKRMLSNIYSKSYLQSSPHMRLISESILFDRLLPIIQEAVSSD

APIEVHDLNQGLTIDFVSAYLFGLANGTNWLQDAPFRRRMLHYYQGRKPYEFYHQEVPNL

VSWTKSLGIRLIPRWCDEANEMLDAWCLDLCDKAEQCLQSTEVSVEPVVYKQLQQAMIKQ

SSQKENTDESLKQQRLDIACELYDQLTAGFETSAVALTYLFWELSRHPDVQEELRA

ELLTLEPKILFPRSSASRGLPQAKAVDSLPLLEAIVTETLRLHAPIPGIQPRVTPYPSC

TLAGYSDIPANIRVNAQAYSLHRNPDVYPDPETWQPKRWLKGVNSDSDLEERRRWFWAFG

SGGRMCVGSNLALQ (1)

EIKLVTAAIYSNYRTSIVDDDNIEPIDAYTVKPRGDKLVLKFEAA*

 

>CYP578C2 Aspergillus flavus NRRL3357

AAIH01000684.1 100% TO A. oryzae

AFL2G_02113

1413 MAGSPIVAISAIVLTITLLYKCIVYPVFLSPLSKIPNAHWTAPISPAWMLWKRFRSQNNR 1592

1593 TIQAAHERLGPIVRLSPSEISINCVEGGIKTVYTGGFEKHEWYPRVFGSLG (2) 1745

1816 TVSMFTMISSKPHSVRKRMLSNIYSKSYLQSSPHMRLISESILFDRLLPIIQEAVSSDAP 1995

1996 IEVHDLNQGLTIDFVSAYLFGLANGTNWLQDAPFRRRMLHYYQGRKPYEFYHQEVPNLVS 2175

2176 WTKSLGIRLIPRWCDEANEMLDAWCLDLCDKAEQCLQSTEVSVEPVVYKQLQQAMIKQSS 2355

2356 QKENTDESLKQQRLDIACELYDQLTAGFETSAVALTYLFWELSRHPDVQEELRAELLTLE 2535

2536 PKILFPRSSASRGLPQAKAVDSLPLLEAIVTETLRLHAPIPGIQPRVTPYPSCTLAGYSD 2715

2716 IPANIRVNAQAYSLHRNPDVYPDPETWQPKRWLKGVNSDSDLEERRRWFWAFGSGGRMCV 2895

2896 GSNLALQ (1) 2916

2975 EIKLVTAAIYSNYRTSIVDDDNIEPIDAYTVKPRGDKLVLKFEAA* 3112

 

>CYP578C3 Aspergillus fumigatus Af293

AAHF01000007.1 chromosome 1 afu1_98, whole genome

74% to 578C1, 43% to CYP578D1, 46% to 578A2

1250155  MVVSIYTSLSIVILLLFVAYKAIVYPVFLSPLAKIPNAHWSAPVSPAWILWKRFRSQNNSTIHAAHE  1250355

1250356  RLGPIVRLGPSEISINCVEGGIKTVYSGGFEKHEWYPRVFGSFG (2)

1250548  TVSMFTMTGSKDHSVRKRMLSNIYSKSFLQSSPHMRLISRTIVYDRLLPILYDAAT  1250715

1250716  SSRPVDVHDLNQGLTMDFVSAYLYGVTNGTNFLQDAPYRQRMLHLYQSRKPFEFYHQEVP  1250895

1250896  NLLSWTKSLGIRLIPRYCDQANEILDSWGLGLCDKAEESLKSNRLEDEPAVYKHLKQM  1251069

1251070  ILKHLPENKNDVEYARILEQQRLDIACEMYDHLTAGHETSAVALTYLLWELSKRPELQDA  1251249

1251250  LRAELSTLEPMVIFPRPSESAELPPAKSIDSLPLLEAIVTETLRLHAPIPGIQPRVTPY  1251426

1251427  PSCTLAGYNNIPPKTRVNAQAYSLHRNPEVFPDPETWEPKRWLKDVNTPSELEERRRWFW  1251606

1251607  AFGSGGRMCVGSNLALQEMKLVVAAVYTNFKTSIVSDDNMEAIDAYTVKPTGESLILRFELVE* 1251848

 

>CYP578C3 Neosartorya fischeri

96% to CYP578C3 Aspergillus fumigatus = ortholog

NFIA_020380 revised

MVVSIYTSLSIAVLLLFVAYKAIVYPVFLSPLSKIPNAHWSAPISPAWILWKRFRSQNNR

TIQAAHERLGPIVRLGPSEISINCVEGGIKTVYSGGFEKHEWYPRVFGSFG (2)

TVSMFTMTGSKDHSVRKRMLSNIYSKSFLQSSPHMRLISRTIVYDRLLPILYEAATSNRPVDVHDLNQGLTMDFVSAYLYGVR

NGTNFLQDAPYRQRMLHLYQSRKPFEFYHQEVPNLLSWTKSLGIRLIPRYCDQANEILDSWGLGLCDKAEESLKSNRLED

EPAVYKHLKQMISKHLPENQNDVEYARILEQQRLDIACEMYDHLTAGHETSAVALTYLLWELSKRPELQDALREELSTLE

PMIIFPRPSESAELPPAKSVDSLPLLEAIVTETLRLHAPIPGIQPRVTPYPSCTLVGYNNIPPKTRVNAQAYSLHRNPEV

FPDPEIWEPKRWLKDVNTPSELEERRRWFWAFGSGGRMCVGSNLALQEIKLVVAAVYTNFKTSIVSDDNMEAIDAYTVKP

TGEELILRFELVE*

 

>CYP578C4  Aspergillus terreus NIH2624 revised 7/18/07

          GenEMBL AAJN01000108.1

          58% to 578C1, 34% to CYP578-un1

          ATEG_03775.1

       MAVSAFTVVALVSLGLFVLYRVIIHPAFI

17000  SPLSKIPNAHWTAPVSPVWILWKRYTSQNNRTIQAAHEKHGPIVRLSPNEISINCVENGI  16821

16820  KSVYTGGFEKHDWYPLGFGSLG (2)

       TVSMFTMTGSKPHSTRKRM  16641

16640  MSNIYSKSFLQSSPHMRLISENIVFSRLLPIFQDAASSGKHLEMHDINQGVTMDFVSAYL  16461

16460  FGLANGTRFLQDEAYRKKMLHLYHSRKPFEFFNQEVPNLLSWTKALGIRLIPRWCDDA  16287

16286  NDILDRWNLELCDKAEASVGSTELDAEPVVYKQLKQAMLKHTSKEDNQNS  16137

16136  VPDTPEKQRIEIACELYDQLTAGFETSAVALTYLFWELSQHPELQTKLREELLTLDPSIR  15957

15956  YPATSKILPSAKSVDSLPLLDAIVTETLRLHAPIPGIQPRVTPYPSCNLAGYTGIP  15789

15788  ANTRVNAQAYSLHRNPEVFPEPETWQPERWLKDGNSSAELEERKRWFWAFGSGGRMCVGS  15609

15608  NLALQ (1)

       EIKLVIASVFTNYRTSIVDDDGIEAIDAYTVKPRSEKLVLKIEVA*

 

>CYP578C5 Aspergillus clavatus NRRL 1

NZ_AAKD03000006.1, 80% to 578C3

ACLA_030420

415154 MGVSTVTSLSIVSVFLFIAYKTIIYPAFLSPLSKIPNAHWSASVSPAWILWKRFRSQNNR 415333

415334 TIQAAHERLGPIVRLSPSEISVNCVEGGIKTIYTGGFEKHDWYPRVFGSFG (2) 415486

415548 TVSMFTMTGSKDHSVRKRMISNIYSKSFLQSSPHMRIISKTMIYDRLLPILYEAATSDTP 415727

415728 VDVHDLNQGLTMDFVSAYLYGLKNATNFLQDTSYRQRMIYLYQCRKPFEFYHQEVPNLIS 415907

415908 WAKSVGIRLIPRWCDQANEILDTWALGLCDKAEACLSSTKLEDEPTVYRQLRQTISKQLP 416087

416088 AKDDPESYAQNPKQQRLEIACELYDHLTAGHETSAVALTYLFWELSKHPELQKALREELS 416267

416268 TLDPKIVYPPPSASAELPSAKSIDSLPLLEAIVTETLRLHAPIPGIQPRVTPSPSCTLAG 416447

416448 YKDIPADTRVNAQAYSLHRNPEVFPEPENWEPKRWLKGINSPSELDERRRWFWAFGSGGR 416627

416628 MCVGSNLALQ (1) 416657

416708 EMKLTVAAIYTNFQTSVISDDNMEAIDAYTVKPRGEKLVLKFQTI* 416845

 

>CYP578C6 Aspergillus niger

e_gw1.1.562.1|Aspni1

75% to CYP578C5

MTPTVSACEDYTDSSTLHHGRTVSMFTMTNNKAHSTRKRMLSNIYSKSFLQSSPHIHLISETILLDRLLPIIQDAASSGS

PIEMHDLDQGITMDFVSAYLYGLANGTNFLQDEAFRCKILRLYQSRKPFEFYHQEVPNLVSWTKRLGLRLIPKWCDEANE

VLDAWGLSFCDKADECLSSTQLNTEPMVYKQLKQSISKQLPPKEAASYNKLIEQQRLDIACELYDHLTAGHETSAVALTY

LFWELSKHPDLQSALREELHTLEPKILYPRPSSSRDLPTPKSIDSLPLLDAIVTETLRLHAPIPGIQPRVTPSPGCTLVG

YSDIPGNTRVSAQAYSLHRNPEVFPEPETWQPKRWLKDCNPSEVLEERRRWFWAFGSGGRMCVGSNLALQEMKLVVAAIY

TNYMTAIIDDEDIEAIDAYTVKPRGEKLVLKFESVA*

 

>CYP578C7 Uncinocarpus reesii

61% to CYP578C2 Aspergillus oryzae

UREG_06580.1

MALVSPTVLAISAVCFLVVAVYKFIIFPAFLSPLSKVPNAHFTSSIAPFWILWKRFQMTGNRTIHEAHQKHGPIVRLAPT

ELSINCVDGGIRTVYAGGFEKHAWYPRVFGAFGTISMFSMVASRPHSIRKRMMSNIYSKSYLQTSTQLSAVSRKILFQRL

FRILEHHVKAVDQVEVHELNNAITMDFVTAYIFGLSAATNFLEDTTTRKQWLDAYQSRKPFEFYHQEVPNLVAWAEKLKL

PLIPSWCKKANDDMENWGLELCDKAEEHLSTADIESQPTVYKQLKMSMIKQLGLKNEKAVPEHMSNQLRMDIACELYDQL

TAGHETSAVALTYLYWELSRNPELQRELRKELRGLSPSINFPIHSETVPNLPSPKDIDALPLLNAVITETLRLHAPIPGL

QPRVTPSPMSSLGGYHQIPPNTRVNAQAYSLHRNLDVFPEPELWLPKRWLKPGDSPEMENMKRWFWAFGSGGRMCVGSNF

ALQEMKLVVAAIYSNYTTTIVDDDGIEAIDAYTVRPTSNRLVLEFHRADI

 

>CYP578C8 Coccidioides immitis

78% to CYP578C7   Uncinocarpus reesii

CIMG_04972.2 revised

MALLSPATLAIITVSALAAGVYKFIIYPALLSPLSKIPNAHFTSSISPLWILWNRFRMRG

NRTIHEAHNKYGPIVRLAPGEISVNCVDGGIRTVYAGGFEKHDWYPRVFGAFG

TISMFSMVGSRPHSVRKRMMSNIYSKSYLQGSPQLAAASRKILFQRLFPILERHVERADQ

VEIHELNNAIT

MDFITAYIFGLSAATNFLEDAQTRKQWLEAYQCRKPFEFYHQEVPSLVAWAETVKLPLIPSWCQKANDYMESWGLEICDN

ADKYLLTTVVESEPTVYKQLKMSMVKQLPSDGVKSLDEETSKKLKLEIACELYDQLTAGHETSAVALTYLYWELSKDPEL

QCELREELRGLSPSIDIPANADIMPDLPSSKDIDSLPLLNAVVMETLRLHAPIPGIQPRVTPSPTCSLGGYDGIPPGTRA

VSAQAYSLHQNPNIFPEPESWLPRRWLKQNDVSEMES

MRRWFWAFGSGGRMCVGSNFALQ

EMKLVVAAIYSNYTTSIVNDDGIEAIDAYTVRPTSNRLILKFGRA*

 

>CYP578C9P  Histoplasma capsulatum G217B

pseudogene

ABBT01000030.1 Ajellomyces capsulatus G217B 99592-98639

51% to CYP578C7

HCB00216.1

AVIAVGILLPCLYK*FLYPVFISPLSKIPNTDISAPASPAWI &

RFRMQGNRTIYKAHQKYGPIVRLEPLEISVNYVDIRIQTVYAGGFEKHDWYPRVFGAYGYVICFDFSASVERPTEGTKPHSVQ &

ISQAIICRRLLPVLQRNIQFQANVEVHGCINAIAMDFVPAYIFGLASGTNFLEDIPTARDWLRVYQSRMPFEFFYQVPRMTYLAK

MLKIPLISNWSDKANQVMENWGLEMIDYAEKFLASTDSACEPVVYKQVKQSMLKQ &

TLKSLKxxxxxxxxQQRLELACEMLTILPLIMKVAQLPLLIFTGSY*GILICR*SFE

ELETLSPKIIAQSVLEIPTELPHPKAIDDLPLLG

AIVMETLRLHSPIPGIQPPITPAPSTTLAGYDKIPRKTRVNVQAYSLHRNHDVFPNPGSFLPDRWLKPMDSVELGNMRRW

LWAFGSGGRMCVGSNLAPQ

SEIKLVVAAIYSNFTSKIIDDDGIDPIDAYTVRPSNNRLVLKFERV

 

>CYP578D1 Nectria haematococca

e_gw1.19.155.1

Necha1/scaffold_19:640595-642276

38% to 578A1 Magnaporthe grisea, 49% to 578E1

in CYP53 clan (part D)

This gene model seems correct

MTSLNIPAVAAALVLLVIVYERIIKPAFLSPLAKIPAPHWSCHVAPFWLWWAKLTHRENGLVYKNHMAKG

KALRLSPGLVSLNCFEGGLKQVYLGGFPKTEFYWRGFANYG (2)

THNIFTFEDNATHSARKRIISGTFSKSFI

LADATSQASMKKILFGRLLPKIDAAAKRNEALEVLELFYSYSMDSFVTWQFGSKLSSNLIEDEKERRLYL

DGFFAAAPYTFWQYEFPRLSSWMKLLGIIPKKVDRGFHDIEQWNLEKCDKAQELLAQGEDKLSDEDKPVV

MSLALKAMSDLHAESRQYPQRLDIASDMFAHNSAAHETSGNTLTYCFYEMSQRPELQAKLRQELLTLDPP

LYFPPPDGGELVLPEPKIMDKLPLLEAVILESLRLYPSVPGGQPRRVPKPCSLGGYDGIPAGTTVQCYAY

SLHRTPEIFPEPDEWKPERWIDSSPEQLSQMRRWFWAFGSGGRMCIGSNFAYF (1)

SMKNVVGSVYTNFTTSLHDHGSMELEDAYLAGPAGHRLEIKFTRVEK*

 

>CYP578D2 Fusarium oxysporum

87% to CYP578D1

FOXG_16972

MTSLNIPAVAAGLVLLLAFYERIIKPAFLSPLAKIPAPHWSCHVAPFWLWWAKLTHQENKLVYKNHMAKGKALRLSPGLV

SLNCFEGGLKQVYLGGFPKTEFYTRGFINYNTENIFTFQDNGTHSARKRIISGTFSKSFILADSTSQASMKKILFGRLLP

VFSRAADSKKPLEVLELFYSYSMDSFITWQFGSTLSSNLIEDEKERRLYLDGFFAAAPYTFWQYEFPRLNNWLKTIGLIP

KKVDSGFHDIEQWNLEKCDKAQELIAQGEEDLSDADKPVVMSLALKAMSDPHAKPGQYPQRLEIASDMFAHNSAAHETSG

NTLTFCFYELSKRPELQAKLREELLTLDPPLYFPPPAEGEWVVPAPKAIDKLPLLEAVILESLRLYPSVPGGQPRRVPKP

SSLGGHDNIPAGTTVQCYAYSLHRTPEIFPEPDQWKPERWIDSSPEHLATMRRWFWAFGSGGRMCIGSNFAYFSMKNVVA

SVYTNFTTTLHDHGSMELEDAYLAGPVGHRLALKFGYIRKM*

 

>CYP578E1 Nectria haematococca

e_gw1.53.20.1

Necha1/scaffold_53:74141-75808

48% to CYP578D1, 46% to CYP578C3, 39% to 578A2

This gene model seems correct

MTLNIPLIAAVAALVLGLYKLVLYPLFFSPLSKIPTAHWSSHVCPLWIYYIRYKNVENQTIHELHKTKGP

ILRTAPSQLAVSCYEGGLKTIYTGGFHKTDFYHNRFINYG (2)

VDPMFAMSGKPHSDRKRMLSHVYSKSYILA

SPTVRETTRAVLFDRFMPIIKSSADSGKPLEVLEILAAYSMDAFMTYQFGLSLGSNFLQNAEERKWYLDT

FYVRRPYLFWTTELPKLTSHLRKIGIKLVPDWVERSTIDLEDWQMRFCDKAENLLAADTRIAAEDNPVVY

AWERSAFKKQDGKNQVLNGQAYPRRLEIASDMYDHNAAAHETSGDTLTYVFYELSRRPEHQDRLRQELSN

LSPPLEYPNAPDELPDPKSVDQLSLLDAILQETLRLWVAVPGGQPRVTPSPSCTLAGYANIPPGVRVQSH

GYTIHRNPEVFPEPDEWIPERWLNQTPERLSEMRRWFWAWGSGGAMCIGSNIATH (1)

SMKHCIAGVYTNYKTTIVCAPDMELAEGFTAGPRGGKLELSFQPV*

 

>CYP578F1 Aspergillus niger

e_gw1.1.426.1|Aspni1

46% to CYP578C4

MEASILPSVPACAGVALVAFFLYHFIIYPSFLSPLAKIPNAHPLAPYTSLWILWARYRIRENNTVLRAHQQHGEVVRLAP

NEVSINCVNDGIKTVYGKGFEKPPFYSVFVNYGALNMFSSLHSPEHAMRKKRISNIYSKSFLQSSPDAAAIIQEVVQRRL

LSQFASHAKSKQPLDIYPIYKATAMDLTTSYFFGLSAGTQFLIDPTAAARWQELYLDDQHPARHLAHDQIAAWCLNMSDG

AEKMLAKKAGGSSLPPGHLPVVYERLRQSTEKEGMSPQQLQVTSELLDQLIANNGTVATTLLYVTWQLSQNSAALARLQE

ELRSKLGPEAFAWSASKEEDTLPKAKDVDELPYLNAIIMETLRLHAPVAGSQPRITPENNKTTLGQYENIPGGVRVSAAA

WSLHRNPAVFPRPDEWHPERWLSENLEGADKKERWFWAFSSGSRMCIGSNLALYLLKYVVAAIYANFRTHIVNDEGIEQE

DGFISGPKGNQLTVRFESITVD*

 

>CYP578G1 Mgr041 Mycosphaerella graminicola

42% to CYP578C4    Aspergillus terreus, 43% to 578A2

MALPIIPLLAALFLLAHLLHKHILHPLFLSPLSSIPPAHWSSHFSSLWILSHRHSQSDTFAIHAAHTRLGSIIRLAPNELSVNSVNRGIRTIYAGGFEKGEWYSEGFANFGVPPMFGMHGAREHGRRKRMVSHVYAKSTLQGSEALGGITKVLVERLRGRLSEAAKT GEQVEMYELFAATMMDFVTGYVFGLGQGTDFLREEKRAREFFKGFKERQRFTFWAQ ECARLMRWVGWMGLRSWFVPKWVDESNEESERWVLEMCDRAEEVVQRQEAGVKKTESKDTPIVYAQLRQSFIKEARKDTKDNPSTTSILQSSHLDLASELLDHVLAGFDTSSITLTYLAWQLSRPSNAHWQSRLRTQIATLNTSL

(short in this region)

DAKLIDNLPILHAVLMETLRLHAAIHGIQPRLTPEGLSSLGDPEAGLVVRDVPGGARVQSQAYSLHRNPEVFPEPEEWRPARWLPSSPASETKVEYEARQREQLRWFWAFSSGGRMCVGSHLAMLDMKATIVAVWGRFDTAIGDDEGMKGVNGGYMAEPLGVFDEEDGQRKRFLRLRLKEL

 

>CYP578G2 Mycosphaerella fijiensis

54% to CYP578G1 Mgr041 Mycosphaerella graminicola

one frameshift = &, possible seq error

estExt_fgenesh1_pm.C_260019

MTLPTIPSAAGVALLALAIYKYIIYPAFLSPLAKIPNAHWSASISPLWILYHRHQQQDTFVVHACHSRLGPIMRLAPNEL

SVNCVDGGIRTIYAGGFEKGDWYSNVFSNYGVEPMFAMEAHGPHSKRKRMLSNVYAKSSLQNSAALTKITDVVIKERLIP

RLQKAAKSGEAVELYDVFSAVTMDFVTGYIFGLRNGSNFVQDYA  &

RWRPALPRIKARQKYTFWPQ

ELPNVTSWLKRLGLKWLVVPTWVDSANFDIESWVMSMCEQAEKTCKRIEQGEDVAPEDTPTVYTQLRNTLLK

DDHNPPASDHRLTLASELLDHTLAGFDTSGITLTWLAWQLSKPVHKTWQHRIRHEISTTPLSSPSHHPLDAKTIDNLPTL

HAILMESLRLHPAIPGNQPRLTPPNTTLAHIRNLPANIRVQAQAYTLHREPSIFPSPEIWDPNRWLLLLSPTTEKNLHRW

FWAFGSGGRMCVGKNLAILDLKAAMVGVWGKFETEIVEDRGMVANGGYMGEPLGVGVDGDGERRRRKFLVVRVREVDV*

 

>CYP578H1 Uncinocarpus reesii

38% to CYP578C3 Aspergillus fumigatus

UREG_04195.1

MLLLAGSLFAIVVVVYKKIIYPLYLSPLAKFPSAHPLASISSLWIQWKRLTDKEFDAVVCGFRQKGPYLRLGPNEVAVNT

MERGVRNIYGVGSDNFDKSPWYNFFKNHGFRNTFCALGSEHAVHRRRISGVYSKSFLQSSPHIRTILDTVIQGRILPILA

RKAKDEETVDILALNLAYGLDFVSAFIFGLPRGVKFLEDRASREHWLELYDRTHPKSLFWLAEYPVLAQVASKFGIPLVP

KGFPKAKRELETWALKRLKLAEDVLAENPTDDTIRPGDMPLLYHALKAGMETEQGKKKNGRFVPNNTQRHELASECLDHL

VATRDTFGITFSFVILNLSRNLEVQEQLRAELRSMTRPFRYHDEKKTEIPAPQALESLTLLNAVIKESLRLRNTGPTLNP

RITPKGRKVTLGPCDDIPAGTRVGAYAWCLHRNEEGYPSPTTWDPTRWIVDASDPKAGERDRWFWAFGSGSRQCLGQNLA

FELMRFAVAGIYTNFKTSIVDDSAFVGDETFVTGDGSEQLILKIERLEN

 

>CYP578H2 Coccidioides immitis

81% to CYP578H1   Uncinocarpus reesii

CIMG_06188.2 revised

MLLLAGTLFTLAFVIYKKILYPLFFSPLAKIPSAHPLASVSSLWIQWKRLTNKEFDAVVSGFRQKGPYIRLAPKEVAVNT

MECGVKNIYGIGNDNFDKAPWYNFFQNHGFRNTFCALGSEHALYRRRISGVYSKSFLQSSPHIRAILDAVIQQRILPILA

RTAEKEEPIDILALNLAYGLDFVSAFIFGLPRGVRFLENTASREDWLDLYDQTHPSKNMFWLTEHPVLSQVASLFGIPLI

PRNFLKAKRELENWAMKRIKLSEDVLSNNPTDETISPGHMPLLYHALKTGIELEQGEKRNGRFEPNNIQRHELASECLDH

LVATRDTFGITFSYVILNLSRNLEIQEQLRQELLSISQPFRYHNEKRTELPTPQTLEALPFLNAVIKESLRLRNTAPTLN

PRITPTGRKVTIGPCDNVPAGTRVGAFAWCLHRNEEGYPNPRTWDPMRWIVDSSDPKAGARERWWWAFGSGSRQCLGQNL

AFELMRFAVTAIYTNFRTSIIDDSAFAGDETFVTGDGSEQLILKIERVET

 

>CYP578J1 Mycosphaerella fijiensis

39% to CYP578G1 Mycosphaerella graminicola

32/33 top hits are ALL CYP578 SEQS.

gw1.9.589.1

MALLVLAAAVLAAAALYHFILYPLLWSPVASIPAVHPLAKISSLYMLWIR

YWDDENDTVFAAHEKYGQVVQLGPQELSVNCIDDGIKTV

YGKNFDRHYFYRVYEDCGRHNLSSSLDATSHAIRKRRVTHIFSKSYLYTSPALSGLLNQIIRYRYLPLVIGYAMEQQPFD

TMILFSALAIDVVTGYTFGTKAGTNFLNDRTQH

AKCIHEIHKGRPYPMMFWLHEFP

GCISFLESIGFLSKERYKSL

EALHMFCLDMCDRAENALADPGSDNTAPEEVPTVYQQFKYTLDKEGLSSTPSLKVLEGHRPISCSPQQLEIAAEVCDQIHASDE

TLAITLTYAVWELSRNVDLQQRLRRECQALGPDIRTDSSASLPAPSLVDSLPLLHAVVMETLRVHPTVAGGQARVTPYGK

LSKLGRYENIPGGYRVQSYARFLHANPAVFPDPNVWQPERWLHSGAKTDLYGPDEKLRWFWAFSSGARMCLGSHLALLVT

KYVLLFTYSNFETYVVNDEGIEHMKGYVGGPKSGKMM*

 

>CYP578-un1  Aspergillus oryzae

            BAE55033.1 AP007150.1

            29% to 578C1, probable pseudogene, last exon may be wrong

MTQWIALLLVAICG

YLALRLRSSSLSKIPAAHWSARFSPIWILWKRYTGKELHTLVDAHQKHGSIVLVGPQD

LSVSCYQDGIRRVYDSGYPKPAPFYSMFNYYR ()

QQNAFTSLDRSEHGLRRRRTAALYSKSAL

1613568 MQSQHLRDITRSITYDRLLPKLNSVAQGNGRIDGLDLSYCICVDYLSSFIFGYSNGTNYLSQPKSAIDVW

RFHYENLMCQESFFVQETPSLYKLLRYISIDLLPRKYTESADFLGRWMSDMASKADRATDRKRSTGLPLA

LEDEPVVYDMAKEAVRKDSPHLSEGDQRKQVASEMFDHIC (?)

Missing I-helix motif here, 7aa

LVLGYAFWYLAQHPDAQQRIQTELNSQGID

MRSRETVTNSSKRPRAVELDSLPYLRAVIDECLRMRPTSTPLPRITPSNRKVSVAGIDGIPPGTRINTFQ 1614560

WFVHRDPQKWDNAHDWNPDRWLTRGNTDNKNEREDVLWAFAS

GPRMCLGNNWTYY (1?)

LTTLNSTAMQHILATICSSFNFTALPREENQCWPGSPEDELPIRVCLRN*

 

>CYP578-un1  Aspergillus flavus

99% to CYP578-un1  Aspergillus oryzae

MTQWIALLLVAICG

YLALRLRSSSLSKIPAAHWSARFSPIWILWKRYTGKELHTLVDAHQKHGSIVLVGPQD

LSVSCYQDGIRRVYDSGYPKPAPFYSMFNYYR ()

QQNAFTSLDRSEHGLRRRRTAALYSKSAL

1613568 MQSQHLRDITRSITYDRLLPKLNSVAQGNGRIDGLDLSYCICVDYLSSFIFGYSNGTNYLSQPKSAIDVW

RFHYENLTCQESFFVQETPSLYKLLRYISIDLLPRKYTESADFLGRWMSDMASKADRATDRKRSTGLPLA

LEDEPVVYDMAKEAVRKDSPHLSEGDQRKQVASEMFDHIC (?)

LVLGYAFWYLAQHPDAQQRIQTELNSQGID

MRSRETVTNSSKRPRAVELDSLPYLRAVIDECLRMRPTSTPLPRITPPNRKVSVAGIDGIPPGTRINTFQ 1614560

WFVHRDPQKWDNAHDWNPDRWLTRGNTDNKNEREDVLWAFAS

GLRMCLGNNWTYY (1?)

LTTLNSTAMQHILATICSSFNFTALPREENQCWPGSPEDELPIRVCLRN*

 

>CYP579 # name changed to CYP58

no sequence.

 

>CYP580A1 # name changed to CYP542B1

no sequence.

 

>CYP581A1 Magnaporthe grisea MG02094.4  poor match AACU01000401 cont2.392

MSIPIATIASAVLSLRGLTWVAFIFLVSYCIYSLHIKPLLSPLRHLPAPK

QGFLWFRLLKEPKAVEIERWMDELPHDGLFRYYGIFNRQRIFTASPKATA

DLLHGPNAFKYIKPKLQFILANNIAGKGLLIQEREENKHARKGLIPAFNP

DQIRKSFPDMWKTAVETIEALPNYTEKMALGDNGCNSTEVVELLKLISAA

SIDIIGHFAYSTDFKAIQINAPKYKRHALKSPNSIFARAYVEMFKTTWRG

QRSLEAASLIGANIALALPLRAVKTIKSIMALVWQVSENIVTEHERAAEY

QNGRDPRGHAQRGDAVRGLSHRDLVTQTVHFLAAGTETVAGSASWAVHLL

SRHPEMQTRLRDEVRAAIPDPNAPQQQATLRSLSYLGAVVQEVLRYHSIN

TLLWRECVDPTASEICGVYIPKGTVVVFSPWAMNRDPKSWGPDARTFNPD

RWLGPADQQEKHRRHPCSFLTFGSGPRRCPGEPYARDELLCLIASLVGRY

HFSPLRPEAESDEGQEIGDDFALTLFKIYEGWRLMVTRVPGW*

 

>CYP581A2 Gibberella moniliformis 7600

AAIM01003085.1 cont2.3085, whole genome shotgun

64% to 581A1

     ERNVEKYVARSDPDMLTLLMQTGHF

77   SHQDLVEQTVHFFAAATETVAGSTCWAIHLLSRHPDIQDRLRAEIREHITSPRSDVSQSQ  256

257  LHSLKYLNAVTNEVLRYHSINTLLWREPTQDITLAGEPIPKGTKIVFSPWALNRDPA  427

428  HWGPDARLFNPSRWLDNPSGGADHAYSFLTFGGGPRRCVGEQYARDQLSCFVASLVG  598

599  RYKFTPVDLQNGSDEGREIGDDFALTLFKILSGWKLNVEVVPGW*  733

 

>CYP581A3 Fusarium oxysporum

91% to CYP581A2

FOXG_12902 revised

MSLSTYLTKPSWIFPILAIVATVLHCINRLFIKPHFSPLKHLPSPKQGPFLFRLIHEPKVSEIETWMDELPHQGLIRYMG

VFNEERIYVASPEAAQELLSSNAYKTIKPELQWILANNIAGHGLLIQEGDQHRQARKRFNPVFSPAQMKKWFPTIWTLAI

DAIDLLPQHIKREPFAPHGVVGIIELVSAASIDIIGRFGFSTEFQTLQRVTSGQSTKGSSDPKVRFGKAYIEMFKTTKRG

QTTLRAASLIGPQIALKLPLRAVKTIDSIMALVRATAEDITTDHERNIEKYVGSPDPDMLTLLMQTGHFLHQDLVEQTVH

FFAAATETVAGSTCWAIHLLSRHPDMQDRLRAEIREHIVSSRSEVSQSQLHSLKYLNAVTNEVLRYHSINTLLWREPTQD

ITLAGERIRKGTKIVFSPWALNRDPAHWGPDARMFNPSRWLDNPSGGADHAYSFLTFGGGPRRCVGEQ

YARDQLSCFVASLVGRYRFTPVDLQNGSDEGREIGDDFALTLFKILSGWKLNVELVPGW*

 

>CYP581A3 Fusarium verticillioides

94% to CYP581A3 Fusarium oxysporum = ortholog

FVEG_11655

MSLSTYLTKPSWIFPILAVVATVLHCIKRLFIKPHFSPLKHLPSPKQGPLLFRLIHEPKV

SEIEAWMDELPHHGLIRYMGVFNQERIYVASPGAAQELLASNAYKTIKPELQWIL

ANNIAGHGLLIQEGEQHKQARKRFNPVFSPAQMKKWFPTIWNLAVDAVDLLPQHIKREPFAPH

GVVAIIELVSAASIDIIGRFGFSTEFQTLQRVTSRESTKGSSDPKVRFGKAYIEMFK

TTKRGQTTLRAASLIGPKIALKLPLRAVKTIDSIMALVRATAE

DITTDHERNVEKYVARSDPDMLTLLMQTGHFSHQDLVEQTVHFFAAATETVA

GSTCWAIHLLSRHPDIQDRLRAEIREHITSPRSDVSQSQLHSLKYLNA

VTNEVLRYHSINTLLWREPTQDITLAGEPIPKGTKIVFSPWALNRDPAHWGP

DARLFNPSRWLDNPSGGADHAYSFLTFGGGPRRCVGEQYARDQLSCFV

ASLVGRYKFTPVDLQNGSDEGREIGDDFALTLFKILSGWKLNVEVVPGW*

 

>CYP581A4P Mycosphaerella fijiensis

45% to CYP581A1   Magnaporthe grisea

N-term not found

fgenesh1_pg.C_scaffold_12000008

LQFVLAQNIASNGLLIQEGEAHKKARKALSP

AFSPAQLRDLHPIAFRTSLEMLERLAGEVNKHSTESVSGGSSTAGEVTPVNILHG

TTLDIVGRWAYSVDFGVVTDPKSLI

RPYIGMFKQSWRGQMTFNVAAVIGPRLALKLPLRAVKTMGAVMKPVHDMSADIVAQH

xxxxxxxxxxxxxxxxx

RG*GSARLDQ

ESLVDQTVHMLAAATETSAGTLSWAVHLLSRYPDWQNKIREEVRRRLPSPRCMN

LEECSSAVQRSDFQDMVHLNAFVKEVLRFHSVNTLLWRECIEPATIASETIQPGTIVSFS

P*ALARDPKVWGPDARTFNPQRWIDDSQGGSRNEAAWLIFGSGPRGCIGEQYAYAQIQAI

IATLVGRYDMSPLNAAVGTDEGHELGDDAALTLSKVMDGWKLKFREVPR*

 

>CYP582A1 Magnaporthe grisea MG04911.4  poor match AACU01000762 cont2.921

MDIQTCAVLSALETVAWVGFHEYRSLALTTRKELVAVFSVFFLVQYLCAK

IYRIFIYPVHVSPLRHLPTPKGNHFMLGQLVNQFRASCPNELYLKWTSQW

PDSPFVRFFTIGNKESLLINGIEANREVFATHCYAFTKPSLFLRFVGDIA

GTGLLFTEGDEHKRHRKMILNVFSVPNLKKMFPIFQEKAEELSQHLDATI

AASTGGSIDVQCIYSKATIDIIGITALGLDLRNLKSTKLRMDFLQCYRRM

HEQPFSAALISFINVHVPIRRLLWFVRANRDFVEASQEVRAMLRTCIRER

IRDVDSRKGAEVKFQSRDLLTYMVEERLLAQCKDPLTTEDILGHLLNFLS

AGHETTTGVMTWASYVLATNPRIQDRLRAEVEALLKESPCPSYSDIERMK

YMHNFVREVLRLYSPAVATYREAAKDVTICGTLIPKGTHLVLCPSVCNKS

KRIWGADAEEFVPERWEKLDGEAASPYSIMTFLAGPRQCIGKQYALQEVK

VLLIEVISKFRLLPTEELVSNDFKVNISNPGVVLRPKGGMVLRVERL*

 

>CYP582B1 Phaeosphaeria nodorum SN15

AAGI01000073.1 cont1.73, whole genome shotgun sequence

39% to 618A1, 41% to 582A1

198750  MYLETCAALSLAELCMLMLMHGVPSMYNVVCSYLVLFSLQYASINLYYTFIFPFY  198586

198585  VSSLRYMPGPKNHHFFIGHALNQFRANGPYELYLSWSRQWPTAPFIRYISFANTETLLVN  198406

198405  SLRAHQEVLSTKCYSFVKPPFFARIVGEVTGMGLVFAEGEDHKRQRKMLN (1?)

        GIFSTPNMKKLLPVVQRHGKRLTETIDQRIGSESSAVVE (1)

198045  VVSLFSKATLDIIGLITLGKELDCFTAKPTFHECYDLIFNQSSLSAIITAVNTYIPL  197875

197874  RSWLPLEANRSFVTANDEVKRILRQQIRLRQKEIATARTSVKKSSLPISRDVLMYLLEA  197698

197697  PPSRQQQWTEDELLGY (0)

        LLNFMSA (1)

197535  GHETSATTLTWAVHVLSTRPDIQTRLRNEIMTLPSCGEPDYTQLEGLRYLHNFCREVLRVYAP  197347

197296  AVMIWRQAAEAVIIENTYIPAGTNVIVSPQVSQSHPEIWGSSATAFEPDRWDSLPPESAS  197117

197116  PYAFQAFSSGPRVCIGKGLAMLEFKSLLVDIVRKYTFEAVEGKLVLENYLTL  196961

196960  RPKGGFRVRFKIAGEDIAQRAD*196891

 

>CYP583A1 Magnaporthe grisea MG05787.4  poor match AACU01000820 cont2.1083

MTSIWSLSSALGHILAIIPDVTHLLSVLLVVILQICLKVSFAPARRPDLS

NIPGPWYTRVSHMWFFYQRWHGRLAPASHAILLSHGDETNGIVRLSPTVV

LLNDMPSLKTLFGKQDAATGTKIVRALNLGGHSWSIMAPEFDLARGRRQA

VQAATTETNMRLWRGALEREVEVLLGALRSGGAVDVVRPLRMLTTKASAL

ALAGTEISDATAHEVLEANGLFNRCVANRLCMPDAILSMLDMLPWREHLL

SHTGLFRTLGSGAKLFTLGEKLNDLAAAKGSNLQVQSSALYLQRKQMRIE

DGVEDTDLLSADTAGGLLAGSETTAATLAWILFELSRRPELAERLRNELT

AADGTTTGADLLDAVIKEELRYRGPVALTSNRIVGAGGATVLGGRYFLPA

GTVVVMHNHSLSRRDPRGSHHVWDPMRWVGAGVGGADAADRCTAPFGFGI

RRCPGKTMAWMVMRKVVAAVVKGFVLQCPAETTDETMEAVELNGNRPRKE

ECRLMFFPRDKSQVA*

 

>CYP584A1 Magnaporthe grisea MG08956.4  44% to 52A1 40% to 52F1 AACU01001733 cont2.1681

MSLSTIIAQVALALIASVWIWRSITLYYRRRKLKLLHGCMPVTKIPDWDP

IFGLGFTYTLIKSDINNHRLINNTVERLQTYGYTHMANVMRHRMFFTCEP

ENLKAIMHGQFDHFDNELRSVPAGHFMGMGIFLADGEKWQRSRALVRPNF

ARDQVADLGSLERHFQILLSIIPKDGSAVDLAPLFFAFTMDSITDFLFGE

SVKALQAHASGAHASSSPWSQKGIEQFAEAWEYAQNDMLFRFILGPARYV

YQDKKANQCVKMIHAHVDRYVEQAVRFRNEELARGGDEKEPKSEGRYIFL

QALAKQTQDRKVLRDELMNLLMAGRDTTAALLSNLFHTFARRPDVWAKLK

NEVMFLEGRLPTYEDIRNMKYVRWCINEFGILGKQASFDTTLPRGGGPNG

RSPIFLPKGSIVLYSMLAQHRRKDLFGEDALEFRPERWETLRPGWNYVPF

NGGPRICVGQQYALTEASYLVVRFVQTFATVESRDPEPWSIGKRLLTCPM

NGTIVSLSR*

 

>CYP584A2 Magnaporthe grisea MG05908.4  40% to 52F1 AACU01000839 cont2.1108

MFNLISSLKVGAALVAAVWIWQRIMLYNHRRKIKRDSGCLPAAKFPDWDP

LFGIGFTYHMVTEDILDFQIMKNTADCLEKYGNTFTSKAMGFNFIWTCEP

ENIKAVLSTQFDQFNNQGRSVGAEDFLGKGIFVSDGEHWQKSRALVRPNF

ARDQITDLVSVEERFQTLISLLPKDGSTVDLLPFMFDFTMDTSTHFLFGD

SVKSLEANAHERSIGHHGQENLDEFSAAWDYAQNDMLSRFVLGPLRYVYK

EKKAARCIKLVQDHVEKYVDEAIRYNSQVRAAGGDEKKEPEERYIFLRAL

AKQTQDRRMLRDELLNILVAGRDTTASLLSNMLHTMANRPDIWTKLKNEV

AFLQGRIPTYEDIRNMKYMRWCINESLRLHTVLALVGKEASIDTTLPRGG

GPDHKAPIFVAKGTFIAYSFLALHRRKDLFGEDAHEFKPERWETLRPGWN

YLPFNGGPRICVGQQYALTEAQYVLIRFLQTFSAIESRDPEPWSIGNRMT

TCPKNGVQVSLRC*

 

>CYP584B1 Magnaporthe grisea MG08494.4  42% to 52F2 46% to 52E1 AACU01001266 cont2.1600

MGVQDFANLFLGGRFSGSVTTAAMLLFLVVVLVVSGVRSIRSISARRRFI

AENNCKPAPAWPQKDPILALDFILKGLANFKQKKFLDGWTKYYEDLGPTF

TTKSAGMLYINTIDPENIKTVLATRFRDFSLAERVEIMGPLLGRGIFVTD

GDEWAHSRALLRPNFAKEQVADLGMIDRHFSDLMALLRPHAADGTTVALQ

PLLMRMTMDTATEFLFGQSTRTLSVEQADSAGNAFSRAFQFSLNEMAHLF

RLGPFRILRRQSRELKDSHDICRAYVDGFVDEAWAWKQGGKTDGRTNFLR

DLVESTDNKQKVRDELLNILIAGRDTVASVLGSLFRVLAQRPEIWAKVRA

EVAQFEGELPRYDQLRDLKYAKYCINEALRLWPAVPLNGRLAVRDTVLPR

GGGPNGDEPVYVPKGSLVNYSVYSMHRRTDFYGADAEEFRPERWEGKIQG

WQYLPFNGGPRICLGQQYALTEMLVVLVRFAQEFSHIESRESTPWQEELH

LTVQPANGVQVALTAANTA*

 

>CYP584C1 Magnaporthe grisea MG03821.4  42% to CYP52F3 AACU01001030 cont2.747

MTPQGLLAVCTGLLLLKIFISFVSRYRRHQILIRQHGCLPPPKAKLKDPI

FGIDVILSNGRAAREHRSLQTFEERFYSIGATYSTKHGFPLIITMEPDNV

RAVINQNFHDYALAPFRQPALKHFLGDGIFTTDGEKWHSSRALLRPNFNR

DQIANLEAIETHVKDLFALLPRDGATPVDLKGLLLRFTMDSTTEFLFGQS

VYSLRRNGARDGDSLEAQFETAFTGAQAECMEFIGMGPLARFARVNSQKQ

VEIVHRYIDKFVDRAIAFRKEYGKQGHRSESSLTGFVPGPAYSQPGYPCH

AFRKEYEAGGGGPIGTGKEKPKYLFLQELAKQTTDRTRLRSELLNILMAG

RDTTASLLGNLFFMISKSPEIMAKLQQEVDSLNGARPSYSSLRDMKYLKY

CLRESLRLHPVAPVNTRIAVTDTVLPRGGGPDGSKPVLVPKGTAVGWSVY

AMHRRKDLYGPDADRFRPERWETLNTRSEYLPFNGGPRICLGQQYALTEA

SYVTVRMLQEYEMESRDPGEWEEELAITACNRRGCLVALKARSE*

 

>CYP584D1 Fusarium graminearum FG08320.1 AACM01000333 FGcontig1.333_scaffold5

MFSLLFLQFFEQHFIATAILLLLPIPLRAIETYLRRWRLAQAQGCQDAAS

KVPVKDPIVGFDFLYKVFFGKAPERYLDSTWQAFKEMGTTYIERRWSWQC

VYTCDPHNLKHILASACEDFDLPEFRTSVIGHVFGQGIFVLSGHTWKHAR

AVLRQSFRKGNPTPFLETLERNFQAFSKHVPTDGSAFDLQPLFLAFAMDV

STEFLMGHSTNMLSDKADHTREQQFVDDYMICSEEIIQQMQLGPLHRLKF

NFAANRAKKRVYEYLDIFIEES LNSPRQGSKVSFLTDMMAIAGDRKGLSD

HILHILLASRDTTSSLLSNLFFFLSKNPRIYNKLRDQVLNVAGHEPPTAS

QLKELTYLKWCVNESLRLHPVIPTNARVAVRDTTIPRGGGADGTSPLFIS

KGTALFYNVYAMHRNKDVFGPEPEEFIPERWEDLRPGWGYLPFNGGARAC

IGQQYALLETHYVVARMAQTYTLLESRDDGDWMEFLDWFQWSIWTEDLMG

ANR*

 

>CYP584D2 Nectria haematococca

fgenesh1_pg.scaffold_1000851

Necha1/scaffold_1:2763714-2765329

79% to 584D1 Fusarium graminearum

This gene model seems correct

MLSPLLTKFLEQHLVLTIVLLLVPFPLRALETYLRRWRLAQARGCREARSKVAVKDPIIGFDFLYRALFS

HAPERYLDSTWAAFKEMGTTYVEKRWTWQCVYTCDPLNLKQILATASEDFDLPEFRTSVIGHVFGRGIFV

LSGHTWKHARTVLRRSFKKENPAPFLETLERNFQAFTSHVPTDGSEVDLQPLFLGLTMDVATEFLMGHST

GMLRNKGDHSREQQFVDDYMVCSEEIIQQMQLGPLHQLKFNFKAKKAKKRVYEYLDKFIDESLQHSKESN

PGSNFLADMMAIAKDRKGLSDQILHILLASRDTTSSLLSNLFFVLSKNPDIFAKLRQEVLHISGQEPPTA

HQLKEMTYMKWCVNE (1)

SLRLHPVIPTNARVAVRDTTIPRGGGADGQSPLLVPRGTAMFYNVYAMHRSEAVF

GPCAEEFIPERWRDLRPGWGYLPFNGGARACIG (1)

QQYALLETHYVVARMAQTYTRLESRDDRDWMELYALALCSKNGTRVAARR*

 

>CYP584D4 Fusarium oxysporum

80% to CYP584D2 Nectria haematococca

bad ag boundary in EXXR motif = gg

FOXG_03506

MFSLLLIKFLEQNLLATIVLLFLPVP

LRILETYLRRWRLAQAQGCKEATSKVAVKDPIIGFDFLYKVFFEKSPERYLDSTWQAFKE

MGTTYIEKRWSWQCVYTCDPRNIKQILATAYHDFDLPEFRTSVIGHIFGQGIFVLSGHPW

KHARALLRQSFRKDNPAPFLDTLERNFQSFLQHVPTDGSEVDLQPLFLALTMDVSTEFIM

GHSTNMLIQNADHTREQQFVDDYMVCSEEIIQQMQLGPLHSL

KFNFAAKRAKKRVYEYLDAFIQESLNKSE

DGPGSSFLAEMMDVVDDRTGLSHHILHILLASRDTTSGLLGNLFFFLAKKPEIYAKLRDEVLSVAGHEPPTASQLKDMTY

LKWCVNE (1?)

SLRLHPVIPTNARVAVRDTTIPRGGGIDGKSPLFVSKGTAMFYNVYAMHRNEDVFGPKAEKFAPERWKDLRPGWGYLPFN

GGARACIGQQYALLETHYVVARMAQAYSELQSRDDEEWMELYALALCSKNGCRVAAKR*

 

>CYP584D4 Fusarium verticillioides

93% to CYP584D4 Fusarium oxysporum = ortholog

FVEG_02372

MFSLLLFKFLEQHLLATIVLLFLPVPLGVLETYLRRWRLAQAQGCKEATRKVAV

KDPIVGFDFLYKIFFEKSPERYLDSTWQAFKEMGTTYIEKRWSWQC

VYTCDPQNFKHILATAYHDFDLPEFRTSVIGHIFGQGIFVLSGHPWKQARAVLRQ

SFRKDNHAPFLDTLERNFQSFLQHVPTDGSEVDLQPLFLALTMDI

STEFLMGHSTNMLIQNADHTREQQFVDDYMVCSEEIIQQMQLGPLHSLKFNFAAKK

ARKRVYEYLDAFIQESLNKSEDGSGSSFLTEMMDVANDRTALSH

HILHILLASRDTTSGLLGNLFFFLAKKPAIYAKLRGEVLSVAGQEPPTASQLKDMT

YLKWCVNESLRLHPVIPTNARVAVRDTTIPRGGGIDGESPLFVP

KGTAMFYNVYAMHRNEDVFGPKAEEFTPERWKDLRPGWGYLPFNGGARACIG

QQYALLETHYVVARMAQAYSEIESRDDEEWMELYALALCSKNGCRVATKR*

 

>CYP584E1 Aspergillus nidulans AN9384.1

MALLGLLETYRDPLAFVAALSAVFYLLSCLHRRWRRYQFAKENGCEPVVG

KIPSYDPFLSLDRLYRLTKLARNRKLLENNVQRFKEFGNTHRAKRITTPM

IVTRDPQNIKAILSLKFGDYSMGDRIRIFGPLLGHGIFTSDGEDWARSRH

MIRPNFVKEQVAHLEIFEELMDDLFALIPTDGSTVDLQELFFGFTIDSAT

EFLCGHNVHSLKKRRSGVIDDEPDFAEAFNYSLDNISKNGRFGPLMFLNR

DPKAVESHRICHESMDQFVDKAIRMRERYNEE

KIVDEAAERRYMFLYGLA

QQTGDRRRIRDEVLNILLAGRDTTASLLSNMFFLLAKHPSTWAKLQEEVA

TLEGRAPTYEQLRNMKYLKYCLNETLRLFPVVPVNSRTAIRDTVLPVGGG

PDGQSPAFVPKGTMVAYSVYAMHRREDYYGADAEEFRPERWADLRPGWEY

LPFNGGPRICVGQQYALTEAAYVTTRLAQRYSILESRDPGPWEEKLTLTL

CSYNGTQVSLRH*

 

>CYP584E2  Aspergillus fumigatus Af293

          GenEMBL XP_749919.1 also EAL87881.1

          64% to 584E1

MTSNYSISLVAGAIAVFVYTLSVLRRKWTQYRFARAHNCQAPPRLASKDPLFGLDTIYATVQNVKEHKFL

ERAQARFDAVGHTIQTRRLLQTIIFTRDPQNIKTILSLRFKDYALGHRYKTFGPLLGHGIFTTDGEHWAQ

SRAMIRPNFVREQVADLAIFEAQMADLLELIPNDGRTVDLQDLFFSYTIDSATEFLFGQSVQSLKKRRSG

GRDAHAEDDFASAFNYAQHAIATRIRLGPLAAFYRDRKVERCNRVCHALVEQFVDKALEVRAQGGRVDEK

TLEDGDGEKKPRYVFLHGLAQQTGDRRRIRDEVMNVLLAGRDTTASLLSNLFFMLAKHPRIWARLRDEVA

PLNGRPPTYEQLRNLTYVKYCLNESLRLHPVVPGNARFAITDTVLPVGGGPDGQSPVFVPKGGIVTYSVW

SMHRREDIYGPDANEFRPERWADLRPGWEYLPFNGGPRICVGQQYALTEAGYVTVRLAQQFAVLESRDPG

PWEENLTLTLCSRNGTKVSLRN

 

>CYP584E2 Neosartorya fischeri

95% to CYP584E2 Aspergillus fumigatus = ortholog

NFIA_022940

MTSNYSISLIAGAIAVLVYALSILRRKWTQYRFAREHNCQAPPRIPSKDPFFGLDTIYATIQNVKEHKFLERAQARFDAV

GHTIKARRLLQTIIFTRDPQNIKTILSLRFKDYALGHRYKTFGPLLGHGIFTTDGEHWAQSRAMIRPNFVREQVADLAIF

EAQMADLLELIPKDGSTVDLQDLFFSYTIDSATEFLFGQSVQSLKKRRSGGRDAHVENDFASAFNYAQHAIATRIRLGPL

AAFYRDRKVERCNRVCHALVEQFVDKALEVRAQGLDGKTLEDGDGEKKQRYVFLHGLAQQTGDRRRIRDEVMNVLLAGRD

TTASLLSNLFFMLAKHPRIWAKLRDEVASLNGRPPTYEQLRNLTYVKYCLNESLRLHPVVPGNARFAITDTVLPVGGGPD

GQSPVFVPKDAIVTYSVWSMHRREDIYGPDANEFRPERWADLRPGWEYLPFNGGPRICVGQQYALTEAGYVTVRLAQQFA

VLESRDPGPWEENLTLTLCSRNGTKVSLRN*

 

>CYP584E3  Aspergillus fumigatus Af293

          GenEMBL XP_747767.1

          54% to 584E1

MHLILHPKTLILLLAVLVTIVCISIRRFLARRQFARQHGCQPV ARSLNKDPFLGLDTIPETLRAASQHRI

LERSVELFRLYGNTFTVKELHKYAILTIEPENIKTILSLNFKDYGLSHRLQPFKPLLGEG IFDTDGAHWA

SSRALIRPSFTRGQVADLTALEDLMQDLFALLPRDGQTVVDLQELFFRYTIDSATEFLFGQSVGTLRKTP

SELGFAQAFHYAQKAIITRGMLGPLAVFYRDRKADECNRVCRAFVQRFVDDAFRAAENNGEKTPDGTKRQ

KRIFSHELAARTSDKRRVLDELMNVLVAGRDTTASLLGNLFFVLAKKPEIWEKLRAEVACLQGRTPTYDE

LRSLRYVQCCVNESLRLHPVVPRNERQAVRDTVLPLGGGSDGLSPVFVPKGTIVSYNVYVMHRRPGLYGP

DAEEFRPERWEDGKLQPRWGYLPFNGGPRICIGQRYALTEVSYVLVRMAQEFRVLESRDPGPWEESLALT

LCSRNGTRVCLVSG

 

>CYP584E3 Neosartorya fischeri

87% to CYP584E3  Aspergillus fumigatus = ortholog

NFIA_049440

MYPSLHPKLVLVLVAALATIACICIKRYLVRRQFARKHGCQPVARSFSKDPFLGLDTIPKTIGALRQHKILERSCNLFRN

YGNTFTVKELQKSAILTIEPENIKTVLSLKFNDYGLSHRLQPFKPLLGEGIFDTDGAHWASSRALIRPSFTRDQVADLTA

LEELIQDLFALLPRDEKTVVDLQELFFRYTIDSATEFLFGQSVGTLKKTHSELGVAQAFHYAQKAIITRGMLGPLAVFYR

DQKADECNRVCREFVQRFVDDAFHAVEGKREKKQDETGRQKRIFSHELAARTSDKRRVLDELMNVLLAGRDTTASLLGNL

FFMLAKKPVVWDKLRAEVACLQGRTPTYEELRSLRYVQCCVNESLRLHPVVPRNERQAVRDTVLPLGGGSDGLSPVFVPK

GTIVSYNLYAMHRRPDFYGPDAEEFRPERWEDGRLQPRWGYLPFNGGPRICIGQRYALTEVSYVLIRMVQEFRVLESRDP

GPWEESLALTLCSRNGTRVCLIPS*

 

>CYP584E4  Aspergillus oryzae

          GenEMBL BAE62566.1

          SHORT part of N-TERM MISSING

          79% TO 584E2, 73% TO 584E1, 55% to 584G1

          revised 3/20/2009

MIMNSSHYPVALLAGAVVFLVYTVTALQRKYIQYRIARANNCQPV

(deletion 87 aa))

IFTTDGDHWAQSRAMIRPNFVKDQVAHLDIFEELMADLLALIPTD

GTTVDLQDLFFCYTIDSATEFLFGHSVQSLKKRLSGVKLDDNDFASSFNYAQDAIAKNTRLGPLRHFFRD

TKAEHCNQVCHELVEQFVEKALKYRANYDEEKAATDDDKKQRYLFLQGLAQQTGDPKRIRDELMNVLLAG

RDTTASLLSNMFFMLAKNPHIWNKLREEVASLEGRAPTYEQLRNLTYLKYCMNESLRLHPVVPSNARFAI

NDTVLPVGGGPDGNAPVFVPKGSIVAYSVYSMHRREDFYGPDANEYRPERWADLRPSWEYLPFNGGPRIC

VGQQYALTEAGYVTVRLAQQFSVLESRDPGPWEENLTLTLCSRNGTKVALRH

 

>CYP584E4  Aspergillus flavus

99% to CYP584E4  Aspergillus oryzae

73% to CYP584E2  Neosartorya fischeri

AFL2G_08031

MIMNSSHYPVALLAGA

VVFLVYTVTALQRKYIQYRIARANNCQPVHCQVNKDPFLGLDSIHNNLDAAKKHVLLERSRSRF

REFGNTFRARRLRTPIIVTCEPQNIKTILSLKFKDYGLGNRIDAFGPLLGHG

IFTTDGDHWAQSRAMIRPNFVKDQVAHL

DIFEELMADLLALIPTDGTTVDLQDLFFCYTIDSATEFLFGHSVQSLKKRLSGVKLDDNDFASSFNYAQDAIAKNTRLGP

LRHFFRDTKAEHCNQVCHKLVEQFVEKALKYRANYDEEKAATDDDKKQRYLFLQGLAQQTGDPKRIRDELMNVLLAGRDT

TASLLSNMFFMLAKNPRIWNKLREEVASLEGRAPTYEQLRNLTYLKYCMNESLRLHPVVPSNARFAINDTVLPVGGGPAG

NAPVFVPKGSIVAYSVYSMHRREDFYGPDANEYRPERWADLRPSWEYLPFNGGPRICVGQQYALTEAGYVTVRLAQQFSV

LESRDPGPWEENLTLTLCSRNGTKVALRH*

 

>CYP584E5  Aspergillus oryzae

          GenEMBL BAE65988.1

          60% to 584E4, 51% TO 584E1

MELLPKLVILLLAALGIASVSIRRFLNRRQFARRCGCQPVARSFSKDPFLGLDTIPGTIRAIRQHRVLGR

SCEIFRAYGNTFTVKELHQSAIVTIEPENIQAVLSLNFKDYTLRHRLELFMPLLGRGIFNTDGQHWASSR

ALIRPSFAREQVANLSLLERLMQDLFVLLPRDSTTVDLQELFFRYTIDSATDLLFGQSVGALKKSQSGLA

FADALQYALKAIPVRDMLGPLNAVYRDRKTDECNRICRDFVQQYVEEAVYAAGPKKEEKESRTTETKRRY

ILSHELASRTSDKQRMVDELINVLLAGRDTTGSLLGNLFFMLAKNPVIWAKLRAEVAVLQNRPPTYEELR

GLRYVQCCVNES LRLHPVVPTNKRKAMRDTVLPRGGGNDGLSPVFVPAGTLVGYNIYAMHRRTDFYGPDA

KEFRPERWEDGKLQPRWGYLPFNGGPRICLGQRYALTEASYVLVRMAQEFRGLESRDPGSWEEGLVLTVC

PRNGTKVGLIP

 

>CYP584E5  Aspergillus flavus

99% to CYP584E5  Aspergillus oryzae

AFL2G_11341

MELLPKLVILLLAALGIASVSIRRFLNRRQFARRCGCQPVARSFSKDPFLGLDTIPGTIRAIRQHRVLGRSCEIFRAYGN

TFTVKELHQSAIVTIEHENIQAVLSLNFKDYTLRHRLELFMPLLGRGIFNTDGQHWASSRALIRPSFAREQVANLSLLER

LMQDLFVLLPRDSTTVDLQELFFRYTIDSATDLLFGQSVGALKKSQSGLAFADALQYALKAIPVRDMLGPLNAVYRDRKA

DECNRICRDFVQQYVEEAVYAAGPKKEEKESRTTETKRRYILSHKLASRTSDKQRMVDELINVLLAGRDTTGSLLGNLFF

MLAKNPVIWAKLRAEVAVLQNRPPTYEELRGLRYVQCCVNE (1)

SLRLHPVVPTNKRKAMRDTVLP

RGGGNDGLSPVFVPAGTLVGYNIYAMHRRTDFYGPDAKEFRPERWEDGKLQPRWGYLPFNGGPRICLGQRYALTEASYVL

VRMAQEFRGLESRDPGSWEEGLVLTLCPRNGTKVGLIP

 

>CYP584E6 Aspergillus niger

fgenesh1_pm.C_scaffold_19000013|Aspni1

67% to CYP584E2

MIALGAALCFFYVIKILRRKWIQSQIIRDTHCEPAALGSKDRLFGIDVLLQNFQAAKNHRLLELIQRRFQQYGNTFRVKR

FATPFIITCEPANLKAVLSVKFKDYGLGSRIETFGPLLGHGIFTSDGEHWAQSRAMVRPNFVKDQIADLEKLEELMLGLL

ELIPTDGSTVDLQDLFFCYTLDSATEFLFGHSVQSLNQLRSGVVSGENSFASAFNYAQHAIARNTRLGFLRRFVRDHKAM

RSNRICHNLVEQFIDKALKYREDYTKKTSGDEERRSRYLFLQGLAEHTGDRDRIRDELMNVLLAGRDTTASLLSNLFFML

ARKPNIWKKLREEVATLNGCAPTYQQLRNLTCLKHCLNESLRFHPVVPVNSRKALSNTVLPVGGGPGGKSPVFVPKGTIV

FYSVWAMHRRQDLYGPDANEFRPERWADLRPGWEYLPFNGGPRICIGQQYALTEAGYVTVRLAQQFSTLESRDSGPWEEA

LTLTLCSRNGTKVCLRN*

 

>CYP584E7 Aspergillus niger

estExt_fgenesh1_pm.C_10613|Aspni1

67% to CYP584E2

MAFDKVDHPVITIATVILVLWCTTTYLQRKWTHRRIIKANNCQPPPSYPQKDPIFGLDILFEQIKYSKKHKILERAIERF

KLYGNTFRQRRFTPFIITCEPENIKTVLSLRFKDYGLAGRIDAMGPLLGHGIFTTDGEHWAQSRAMVRPNFAKEQVAHLD

IFEELVNELISLIPTDGRTVDLQELFFELTIDSATEFLFGHSVHSLRKRRVGALDSTEQDFASAFNYAQKAIVADLRLGR

LKYLRRDRKVGECIRICHELVEQFVDSAMRVREQSSEQKAPPVGDSHDAKEKHKYLFLHGLAQQTGDRRRIRDELINILL

AGRDTTASLLSNLFFMLAKNPRIWNKLREEIAFLEGRAPSYEQLRNLTYVKYCLNESLRLHPVVPANLRFANTDTVLPRG

GGPDGKSPVFVPKGCTVAYNVYALHRREEIFGPDANEFRPERWAEIRPGWEYLPFNGGPRICVGQQYALTEAGYVTVRLA

QKFSVLESKDTGQWVEGYALTLCSRNGTKVALS*

 

>CYP584E8 Aspergillus niger

estExt_fgenesh1_pm.C_40266|Aspni1

76% to CYP584E5

MEFHPKLIILLLTALVIAGESFRRYLKRRQFARRHGCEPVARSFSKDPFLGLDTILGTMRTRREHRILERSCRLFRTLGN

TFTVKELGRSAILTIEPENIKTVLSLNFNDYSLKHRYEAMKPLLGEGIFNTDGHHWATSRALIRPSFAREQVTDLRLLEE

LIQDLFALLPRDGSTVDLQELFFRYTIDSATEFLFGQSVGALKESHSEVGFAEAFHYAQMAIPMRGMLGPLGVFFRDRKA

EECNRICRDFVQRFVDEALYAAATRKEDEATETKRKYIFSHELASRTSDKQRILDELMNVLLAGRDTTACLLGNMFFVLA

KNPAIWARLRAEVESLQSRPPTYEELRGLKYVQCCVNESLRLHPVVPRNEREAAKDTILPVGGGKDGLSPVFVPKGAVVG

YNVYAMHRRPDIYGPDAEVFRPERWEDGKLQPRWGYLPFNGGPRICIGQRYALTEVSYVLVRMVQEFRSLESRDPGPWVE

GLALTLCSRNGAKVGLIPSHTN*

 

>CYP584E9 Mgr002 Mycosphaerella graminicola

61% TO CYP584E3  Aspergillus fumigatus

MAPHTTLLLCTTLALLGYATWVHIRYRLDCRQFARAHGCKPTPKSYGTSIILGLDTLPDLIRAIRQHRLLDLGCEVFERYGHTFSATELGRHHIVTIDPDNIKTVLSLQFKDYAVGYRLDAFRPLLGESIFDTDGEAWARSRTLIRPSFARDQIADLASLESLFQDLLALLPRDGKTVVDLQPLFYRYTLDSACDFLFGRSACTLRGEHSGTDFESAFEYAKQAMLVRGTIGRLGMLYRDRKAEKAYRTCRDFAGQFVDEAIRKASGSGTTAEHDSSKRIFSHELAVRTTSRKVVLDECMSLLLAGRDTTASLLGNLFFMLAKNPAIWDKIREEVAFLDGRPPSYDELRQLRYVQRCVNESLRLHPVIPRNERQAVRHTVLPNGGGIDGSSPVFVAKGRVVAWNIHAMHSRKDIYGPDADEFRPERWENEELRPQWAYLPFNGGPRICIGQQYALTEAGYVLVRMAQEFSVLKSEDDGPWVESLAITVFPRNGVKVSLTPA

 

>CYP584E10 Aspergillus clavatus

75% to CYP584E2

ACLA_032820

MASHRFSLLLVAVLLAVYILSVLHRKWTQYRFARAHGCQPPVRLPLKDPFFGLDLMLQAIRNSREHKSLERTVRRFDTYG

NTIATRRFATRMIFTREPQNVKTVLSLRFRDYGLGRRINTFGPLLGHGIFTTDGDHWAQSRAMIRPNFVREQVADLHVFE

SLLVDFLALIPADAATVDLQDLFFSYTIDSATEFLFGHSVQSLKKRRAGGRDAGSEDDFAAAFNCAQQAIVNGNRLGPLR

FLFPDRRAEAARRVCHALVEQFVDKALAVRATPDEEKAQPPAAEKKRYVFLDGLAQQTGDRRRIRDELMNVLLAGRDTTA

SLLSNLFFMLAKHPRVWARLRAEIATLDGRPPTYEQLRALTYVKYCLNESLRIHPVVPGNARFANTDTVLPLGGGPDGQA

PVFVAKGTVVVYSPFAMHRREEFYGPDAEEFRPERWADLRPGWEYLPFNGGPRICVGQQYALTEAGYVTVRLAQRFSVLE

SRDPGPWEESLALTLSSRNGVKVSLRE*

 

>CYP584E11P Aspergillus clavatus

pseudogene ~70% to 584E3

ACLA_051510, ACLA_051500 (join)

MSILAAVVFIYRLARNCETSCLYGNTFTVQELQRRAIAVIEPENIKTVLSLNFKDYGIRYCLELFRPLLGEGIFNTDGDH &

AASRALIRPTCA & DPADLRLLEGLLQDLMVLLLRDGTTADL*ELFFRYT

IYSATEFLFGQSVGSLKKKKEEDSSSLSELGFVEAFHYAQKAVLTRVMLGSFTAVY

RDHKADVCNRICRDFVQQFVEDAVQAAEGRRKEDDLLGEERMKTKEKRIFSYELASQTSDK

RRILDELMDVLLAERDTTASLLGNLFFMLAKNPAIWEKPRREVDVLQNRSPTYKELRRLKYVQCRVNEYIDLSP

ERWEDGELHLQLLPRWEYLPFNGGPRICIGQRYALNEVSYVLVRMVQKFRGLESRDPGPWEESLA*

 

>CYP584E12 Aspergillus terreus

77% to CYP584E6

ATEG_02893.1

MQTDQGHFSVAALGVAVVVVFYVLNVLQRKWKQARFIKENRCESVALSTESRLFGINQLRENVDAAKSHRFLTLTEQRFQ

RYGYTFRAKRFATSFVNTCEPLNIKTVLSLKFKDYSLGGRIDTFGPLLGHGIFTADGEHWAQSRAMVRPNFVKEQVADLE

KFEELMQGLLELIPTDGSTVDLQDLFFCYTIDSATEFLFGHSVQSLQKRLSGVSLDENDFASAFNYAQDAIAHKARLGFL

RRFVRDPKADHANRVCHELVEQFVDKALKYREHYDEEKAEGDEAKRARYLFLQGLAKQTGDRDRIRDELMNILLAGRDTT

ASLLSNMFFMLARNPEIWNKLREEVATLEGRAPTYEQLRNLTYLKYCLNESLRLHPVVPINSRRAIRDTVLPLGGGPDGK

SPVFVPQGTIVTYSVWAMHRREDLYGPDASEFRPERWADLRPGWEYLPFNGGPRICVGQQYALTEAGYVTVRLAQQFTTL

ESRDPGPWEEGLTLTLCSRNGTKVSLRR*

 

>CYP584E13 Aspergillus terreus

63% to CYP584E12

ATEG_01150.1

MVISALVSVGPTTFLKALFGFVALYFAILAIKQKLALRASAGHAVRTRGR

PFYVGLDMLWETLTHAKKHQFLDLQRTRFR

RYGNTFSTKRLATTVITTCEPSNIKTILSLRFKDYELKRLSSFGPLLGKGIFTTDGAEWMKSRAILRPNFDKPQLVNLEM

FESLMQEMFKLIPDNGDTVDLQKLFFCYTVDSATHFLFGHGVHSLRKLRLGSAGDDTEIQFATAFNYAQGSARIRHRLGP

LKALYHDRKAMECNKICHQLAEECIDKALQAQADGASGKKKRYIFLEELLAQTNDRRRIRDELLNILLAGRDTTASLLSN

LFFMLARHPHIWQKLRTEVANLDGQTPTYDQLRSFKYLKYCLNESLRVHPVIPVNARFATKDTVLPVGGGPDGQAPAFVP

RGSMIVYDVYNMHHRQDLYGPDADEFRPERWEHLRPRWEYLPFNGGPRICLGQQYALTEAGYVTVRLAQRYRTLESRDPY

PWVEDLTLALSSKNGTHVSLMT*

 

>CYP584F1 Nectria haematococca

fgenesh1_pg.scaffold_53000004

Necha1/scaffold_53:9312-10650

MQLTLTLLPVALAAAVAYFIAFQVTVYRRRRSHGCKPAPHAMAWDPIFGLKHATALHKAIRNHRNLEYFD

KMFKSYGNTFEVGLLGNLMHVTCDPENIKAILATKFADW (2)

(seq. gap about 184 amino acids)

(1) VLAAVGSEASLISTLFFVLARYPEVQEKLRQEMSNTLEGDRMPTYDDVRNMK

YLNWVIKEVLRVYPPVPQNLRVANKDTILPVGGGPDGRSPVFIPKGHECSFSSYSLHRREDLWGKDSLDF

RPERWEKERPTWSYIPFSGGPRICL (1)

GQQLALVGAGYVLLRFMQQYPTFKGPQPIQEWEEKLNITCFVNNGVWVNIG*

 

>CYP584G1  Aspergillus oryzae

          GenEMBL BAE59590.1

          55% to CYP584E4, 49% TO 52H1, 44% TO 584B1

          47% TO 584E1 49% TO 584E2

MAVSLSFLTYLFLIVAGVYLARKISRAVQIRQFKKRHGCLPPPRDHHKDPVLGLDEVRSMLRVFREDYLM

EYTLEKYRRHGNTFATSVLGDDDIFTAEPENIKTILAVKFKQFDLGETRRRTFHPLLGDGIFAADGPQWE

HSRTLLRPSFTRTQIAATDLHERHIQRLISRIPRDGSTVDLQELFFNLTLDTATEFLFGESVESLRLGSS

AGSSSFAHHFN VAQDEIAFSMVIAPFDQLIFRPRFRESVREARGYVGN FVKKAIEYRHSLDAEKHAGDTT

DSQSRYVFLEELAKETDNPSDITDQILNILLAGRDTTASLLSMVFYNLARRPDIWDLLRSEVATLDGKCP

SFEELKQLKYLSWVINETLRLYPVVPSNSRTANEDTFLPVGGGPDGKSPVFVAKGQRVAYDVYVMHRRHD

IFGPDAEEFRPERWETIRPGWGYLPFNGGPRICLGQQFALTEASYTTVRIVQSFKEITSRDPEPYRERLA

LTLASRHGTKVAMVPA

 

>CYP584G1  Aspergillus flavus

99% to CYP584G1  Aspergillus oryzae

AFL2G_07147 revised gene fusion

MAVSLSFLTYLSLIVAGVYLARKISRAVQ (1)

IRQFKKRHGCLPPPRDHHKDPVLGLDEVRSMLRVFREDYLMEYTLEKYRRHGNTFATSVLGDDDIFTAEPENIKTIL

AVKFKQFDLGETRRRTFHPLLGDGIFAADGPQWEHSRTLLRPSFTRTQIAATDLHERHIQRLISRIPRDGSTVDLQELFF

NLTLDTATEFLFGESVESLRLGSSAGSSSFAHHFNVAQDEIAFSMVIAPFDQLIFRPRFRESVRVARGYVGNFVKKAIEY

RHSLDAEKHAGDTTDSQSRYVFLEELAKETDNPSDITDQILNILLAGRDTTASLLSMVFYNLARRPDIWDLLRSEVATLG

GKCPSFEELKQLKYLSWVINETLRLYPVVPSNSRTANEDTFLPVGGGPDGKSPVFVAKGQRVAYDVYVMHRRHDIFGPDA

EEFRPERWETIRPGWGYLPFNGGPRICLGQQFALTEASYTTVRIVQSFKEITSRDPEPYRERLALTLASRHGTKVAMVPV*

 

>CYP584G2 Neosartorya fischeri

56% to CYP584G1

NFIA_063580

Note: this seq has no ortholog in A. fumigatus

MVQNSHQTGKRRKQSRTDDEIERKYAHKHGCRPPKHSVSLGPFGLGMIMTMLAATREHRFLEQVRSWHRAYGTTFKATMI

VRKAIFTVEPKNVQTVLALKFKDFELGEYRNRAVRPLLGYGIFSTDGSVWEHSRALLRPNFTRNQIADISVYETHVAHLI

KQIPRDGSTVDLQDLFFRMTLDSATEFLFGESVHSLGDAASAPASSFAADFNASQRGLALRSRLGSLMMFYRDKQFSNAV

ADARSYVDRFVQKAIDYRIALDNGQYVSEEARKVNEQQYVFLHELSKRTLNKTELTDQLLNILLAGRDTTASLLSITFFT

MARRPDIWNKLRQEVLALGGRKPSFEDLKSMTYLTWVLNETLRLYPVVPINVRMANKDTVLPVGGGHRGKDPVFVPKGYE

VIYSVYTMHRLPEIFGDDADEYRPERWEKLKPGWAYIPFNGGPRICLGQQFALTEAGYTTVRLMQQFEAIESRDPKPLTE

GLTLTLASLNGTKVAMKPAKGS*

 

>CYP584H1 Aspergillus niger

e_gw1.19.73.1|Aspni1

44% to CYP584E2

MALHGLHALAVIALLWPLLLQAIYRAFRASSCQRFRLLHGCQSAPKGGRRSLPFGIDNLAVLWRATRENRVLETVESDFR

SSGHTTMVQRHLNRRMVITCEPENIRTILSLKSEDYSVESRNQAFGPLVNTGILISDGVTWAHSRGILRRNFTRSHLANF

EPLEQTLQDLISLLPRDGVTVDLQPLFLRYTLDIASGLHLGQSLHSLRQKAASNGKPTRIDEFKEAMGHAAKDVARRHHL

GVLRYFFIDLRARKSYRTYQAFVDQLVEEALRDSKKSSLASESSCASSGPYVALHEWLEQTTDRRRLRDEVILMLLTSGD

TTASLLGNLFSMLARHPTVWQKLRAEISSTLQGRRPTYEELPRLQYLRYCINETLRLIPSIPLVDRVARRDTVLPVGGGP

DGKSPLMVPQGSQMVCCMYSLHRRVNHFGSDAEEFRPDRWATRQPGDEFMPFSTGPRACPGRQFALTVTEYVTVRLVQLF

PRLEGRDAQPWQGDLSAMLLPKNGVKVALWS

 

>CYP584J1 Mgr036 Mycosphaerella graminicola

51% to CYP584E2  Aspergillus fumigatus (yellow region is long)

41% to Mgr002 548E9

MADLTKLSATAQLATGIACLYIFYLLTTTTSIWLRRRSYKRSKGVMPAVRIPAKDPIMGMDLFLRNIRALKDHTLLELGMARFANLKANTIRGLGLGREIHGTIEPENLKTIQAVDFKKWGLGTGRKTGFHPLLGQGIFTSDGPDWQHSRDMLRPNFVRSQIGDTETFEKHISHLIAAIPKDGTTVNLSDLFFRLTIDSATEFLFGESTESLTKDSTDGFAEAFNNGQDFIANKARWGKWARFAPKNKRFEADRKFVHDFVDYYVDKGLAKRAQLLTEDKKPQRYVFIDELVRQTTDRVRIRSELLNILLAGRDTTASLLTNVWFILSKRPDIWAKLQSEVGQLNGVRPTFEQLKDLKYLQSILSESLRLHPVVPLNSREAQCDTTLPVGGGPSGLAPIFIPKGGIVSWNVYAMHRRKDIYGPDAEDFTPERWLD

DPETGKKG

LRPGWGYLPFNGGPRVCLGQQFALTEASYATVRLCQAFSGIESRDPEEWKEFLMLTCVSLNGAKVKLTPREG

 

>CYP584J2 Mycosphaerella fijiensis

67% to CYP584J1 Mycosphaerella graminicola

estExt_fgenesh1_pg.C_90431

MEVSHMSHTTRAALGLATLYIAYQVITSITLWARRRAFKREKGVMEAPWYPQWDKITGLNLFRKNVQALKEHRMLEVGHR

RFSETSSNTYQLVLLGQHMHITTEPENLKIIQAKEHTKWSLGRRRKYGFRPLLGEGIFTTDGSAWQHSRDMLRPNFVRSQ

VADVDTFEKHVSQLISIIPRNQTVDLSELFFRLTIDSATEFLFGESTNSLASNTTDGFADAFTHGQDFIANLARWGSIGK

LFLATKRFEKDTKFVHDFVDYYVQKGLARRDQILAEKTIGDGKSGRYVFIDELVKQTTDPIRIRSEALNILLAGRDTTAS

LLTNVWYTLSNRPDIWEKLQAEVKQLDDARPSFEQLKDLKYVKALLNESLRLHPVVPLNSREATEDTTLPLGGGPDGRAP

IFIKKDSVMAWSVWAMHRRKDFYGEDAEEFKPERWLDDPVTGKKGLRPGWEYLPFNGGARICLGQQFALTEASYCTVRLC

QAFSGIESRGPNPWTEFITLTCVNLHGAKVALTPRET*

 

>CYP584K1 Uncinocarpus reesii

47% to CYP584G1, middle region similar to EST from Coccidioides posadasii GH403863.1

UREG_00396.1

MLNTTNTALILVAAGALHFGYRYVRSYLEIERIRKMENAEEPPSDPDEGLLGIAIYRRMAKDQGEHVFLEEQTKLYERLG

DTYKANLFGRPFIDTRDPENIKAILSTQFEQFSLGESRLDAFHPMLGDGIFTHVYGGGPKGEPWRHSRAMLRPQFAKQQI

QDLENLEGFVQTLISRIPGNETCDLQELFFRLSMDTATDFLFGESVNSLLPDSPPRETRFYHCFSRGT (1)

EICMLRSLLGDLYFLQKDGREFKQISAFVKEYIDRFTYKALQLH

ADGKPTSYDANGKYIFLEEISKSIKDPVRLRSELLNILLAGRDTTASLLAICFHQLARHKDVWNQLRNEIMENIGHRHPT

YEDIKSLKYLKYVLNETLRLFPVVPWNGREAVVTTTLPRGGGPDGKSKIVVKKGTFVFYSPWSLHRSKFYGEDANEFKPS

RWETLRPAWNYIPFNGGPRICLGQQYALTEASYVVIRLLQAFKDIDNRDPIEPFIEHTTLTLSSHTGTKVALTPA

 

>CYP584K2 Coccidioides immitis

80% to CYP584K1

CIMG_00331.2

MLNLTNVLLIPPAAGALHVGYRYLQSYLEAERVRKLENTEDPPKAPNQGIFGINVYRDMARHKEEHVFLQKNLERYDELG

DTYTSDMFGRTYIDTRDPENIKAILSTQFEQFSLGDSRLDAFYPMLGEGIFTHVYGGGPKGEPWRHSRAVLRPQFARHQI

QDLAKLEEFVQNLISRIPRNETCDLQELFFRLTLDT (1)

ATDFLFGESVNS

LLPDSPPKETQFYHDFTRGTEICMLRTLLGDLYFLQKDSSEFKRVSAFVQEYIDRFTHKALELHAAGKPTSY

DSNGKYVFLEEISKSIRDPVRLRSELLNILLAGRDTTASLLSACFHQLARHKDVWDELRKEIMDNVGDRLPTYEDIKSLR

YLRYVLNETLRLFPVVPWNGRQAVVTTTLPRGGGPDGKNKILVKKGTFVLYSTWGLHRSKFYGEDTNEFRPSRWETIRPG

WNYIPFNGGPRICLGQQYALVEASYVITRLLQVFKDIENRDPVLPFVEHTTLTLSSEHGAKVALTPA

 

>CYP584L1 Uncinocarpus reesii

49% to CYP584G1, 45% to CYP584K1

UREG_01634.1

MLSALKDAPPGHLALGAVACFILYQVYIYLTVGASRRKFAADHGCQPAPAYPHREPIFGIDLA LKLARYGKSKTFLEQAR

NRFLAYGNTYSLKVMG KNAITTCEPENIKATLATKFQDFNLSNVRKAAFQPLFGKGVFTNDGPEWEHSRTMLRPNFVRSQ

VADLDMIEKHVQNLIKEVPTDGSTIDLQDLFFDLTLDTATEFLFGESAHSLGSKSTEENERFSDLFTYCTTMIGDWSRIG

IFFGIPDQRYHRFAAGIHKFADRYVEKALAHHRAQKSGMITCENSPNRYIFLDELVKKTNDPIELRSEALNILLAGRDTT

ASLLACLWNVLSKRPDVWSKLRAEVDSLGGKKPSFEELKDMKYLRYCINEALRLYPVVPSNGRVAIRDTFLPVGGGKDRK

SPIFLKKGTLVSYSVFSMHRREDIYGPDAAEYKPERWEKLRPGWEFLPFNGGPRICLGQQLALTEASYTTVRLMQHFRDV

ESRDSHPWTELLTVTCASNYGAKVALKP

 

>CYP584L2 Coccidioides immitis

76% to CYP584L1, shortened the N-term

CIMG_04399.2

MLSALKDIPPGHLALGAAACFLLYQVYLYLTLGLARRKFIQQNGCRP

PPAYPHKDPILGLDWALKGMKYSRSRTFLQGTQKRFREHGNTHSLNVIGKPAIATCDPENIKATLATKFQDFDLSTVRKR

LFQPLFGKGIFTTDGAEWEHSRAMLRPNFVRSQVADLDMIERHLKNLFKEIPTDGSTVDLQDLFFDLTLDTATEFLFGES

AYTLTKKDMGSGERFSDIFTYCTAVIGQWARLGIFLGIPDRRYHRYAAGVHEFADRYVQKALENYRAQQSGMITSDKTSN

RYIFLDELVKSTQDPVELRSEALNILLAGRDTTASLLACLWNVLSKRPDVWSKLLNEVDALNKKRPSFEELKNMKFLRYC

VNEALRLYPVVPGNTRVAVRDTFVPVGGGPDGKSPVFVPKGTLLNYSVYTMHRREDIYGPDAAEFKPERWEKLRPGWEYL

PFNGGPRICLGQQLALTEASYTTVRLMQEFRHVESRDPNPWEELLTVTCASAHGAKVALRP

 

>CYP584L3 Histoplasma capsulatum G217B

ABBT01000202.1 Ajellomyces capsulatus G217B

64% to CYP584L1

HCB05383.1

586513  MLSLIHVVVGAFGCFFLYQVYLYFTVGAARRKLVKEHACRPPPAYPHKDPIFGLDLTMKLRGHKQ  586319

586318  KNILLQESVKRYRIFGNTFSLI (1)

586149  AISTCEPENIKCVLSLKFSDYEINALRKTAFHGVFGQGIFTTDGPQWEHSRTMLRPNFNRAQVAELAIFEAHIQ

NLIQVVPTDGSTVDLQPLFFDLTADTATEFLFGESANTLHKNFKPSTEENFAEAFTYCTTEIGNSLRVGLLDRYFKTDKR

FERDRKLIHDFADRYVKKALENHANEKYGSGPLPEKGRYVFLQELAKQTQDPIALRSETLNILLAGRDTTASLLANVWNI

IPKRPDVLKKLRAEVDQLGGRKPTFEELKNMKYLKYVLNECLRLWPVVPNNARAAIRDTILPLGGGPDGKSPVFVKKGTT

LAYSVYAMHRRQDIYGPDAAEFRPERWETIRPGWEYLPFNGGPRICLGQQFALTEASYTTVRLMQHFRTIESRDPEPWTE

WVTITCASHHGAKVSLKP* 584657

 

>CYP584M1 Aspergillus clavatus

44% to CYP584E2

ACLA_055810

MWQYLLGSILLLVALHLVKRVEQWRNRRRYAQKYSTKDPVRISDIRRPEGGSYTKETVQAFRKHQGLELLRKRHEACGYT

FESHTLGGRIVNTSEPENIKAVLATHFQDYSLGFRQAALGPLLGKGVFTTDGQEWKHSRGLVRPNVEKAHVTDLSILEKH

IRQLLAHIPRDGRTIDVQDYFFKLSFDNSTEFLFGESVRSFEAAENSEEARFAAAFDFASGETIRRLQYGPFLPLYFNRD

FTKACKVVHRFVDNVVAKALARRASRKAGEKTGNYNFLDGLMDSVTEPKRLRSETLNLMQAGRDTTAGMLGHVFYLLARR

SDVWAKLEAEVATLEGRAPTYEDLQQLTYLRQVMNETLRIYPVAPINSRVAVRDTILPTGGGPDHKSPIFIPAGQKISFP

VYVMHRRKDIYGPDANEFRPERWGEPNFRPGWAYLPFNGGPRVCLGQQFAMTEVGYTIVRLVQTFKAVIQRDFSPYKEHV

RISASVYGGVQVGLIPRD*

 

>CYP584N1 Mycosphaerella fijiensis

44% to CYP584E12 Aspergillus terreus

44% to CYP584E10 Aspergillus clavatus

gw1.8.206.1 revised at N-term

MLPLLLSGLLVAFVLNIICKRASLASRRKFFEQKHACQPPKHYL

ATPWWDQYGLNLMRSMLAAAKKSQILEYVHLLTQIHDPEKRGFGNFVAVSRFGTRKTVIS

SDPENVKSVLTRADDFLVEPSRLAAFEGLLGKG

IFTSDGAFWQHSRAMLKPQFDKSQITELDQFERHISKLLALIPDDGSTIDLQPLFHRLTMDTSTEFLTGTSANSLDADEE

ASQEARDFVTAFDLAMEAGTLHARLGKLYNLFPHPAARRANATSRAYIQKWVDRALKNQAERAEKAKKGEQYVFIDELVK

HDEVDRTRALNETMNILLAGRDTTATLLTHLFFNLAREPEVWSKLLAEIDELNGELPSYEKLRDMKYLRYCTQETLRLHP

PVPMLSKAASRDTTIPHGGGPDGLQPLFLAQGDNFFYSTYALQRSEAIYGKDANIFRPERWSDANLRPFWAYIPFGGGQR

VCLGQQYALAETYYVTVRMMQTFRKIEDRDGGVWVEKVAATLGSGNGCLVGL*

 

>CYP585A1 Magnaporthe grisea MG10071.4  38% to 52F5 AACU01001328 cont2.1928

MSNFSFQLNSIALLITLTALLLIYKLLNSFVIRPLHNHLAARRLNCGSLP

TEPSKWPFGIDTVLRGLQADRDQQMPDYVASRFAAMGRYTWRISVLGTSN

IITAEPRNVQALLATQFDDFSMGDARTTNLRILLGRSIFAVDGAAWHGAR

ETMRPLFARDNVSNVELLEGNFQKLLRCIKQELHKDAEGRLWTAPTSLAA

LLPSFTLDSASEAFLGSSAGALDARLDGSAQPHLLWALERMAPLLRVRFA

LRGLYFLYGTRELRRCADAMHAFVDAAMTRADDALKQDHDGQHLGGKRFE

FLQKLRARCADRAEVREQVLGLLAAGTDTTAALVGWVFYSLIRHPRVFAR

LREAVLEDFGATGRQVTFERLKSCRYLQQVLSETLRRHSVVPLNSRRARR

DTTLPTGGGADGTQRVFVPAGTEVNFSTHVLHRRGDLWGRDADDFRPERW

AERRPGVFHYVPFNGGPRICIGQQFALTEAGFVVVRMLQEFDAVEGLHVD

PERDWHNFGITCSPGSPEDRHAGVTCRLRLAS*

 

>CYP585A2 Alternaria solani

Alt2 BAD83681 65% to 585A1 Mg

PKS for alternapyrone is next to three P450s called alt1, 2, 3

Chem Biol. 2005 Dec;12(12):1301-9.

1 mqthllilaa vvlliihivn nflikplqtl lksrrlgcgp vpfeptrwpl didtirrslk

       61 adkeqrtpdf vagrfetmgr ytwglsllgt snlitaeprn vqallatqfd dfimgtarrt

      121 nlktalgrsi favdgkawhr aretmrpifs renvsrlell eehvqtmlqi ietkdegltt

      181 dandrawsap vslavllprl tmdsatelfl gqsthslkka larqqqksgn eehdadsfdh

      241 afermlailg trmrlrslyw lygnkelqkc inalhafvds aidaadqark sgssqlrydf

      301 letlrtrcsd raevreqvlg llaagrdtta sltawvfycl vrnprvykkl rdtvlaefgp

      361 ystnpgqkit feklkgctyl qhvlnetlrl hsvvpfnsrc aardttlpvg ggpdgsmpvf

      421 vpkgtevnfs thvlhrrkdl wgedadefvp erwekkrpgm twqyvpfngg pricigqqfa

      481 lteagyvlvr mvqrydvieg ldidverdwh nftvvcspgs pvardaavmc rlrvave

 

>CYP585A3P Fusarium oxysporum

59% to CYP585A1, stop codon, frameshift = &, no start Met

FOXG_10811

LSIHLLNLLCKDVSHAHRAGQRGCLPAPLEPSKLPFGIDTLLASLRAD

RDQRTPDHVANRFAALGVYTFR

MSILGTTNLVTAEPRNIQAILATQFNDFGMGATRSTNLKTVLGRSIFAAD

GASWRAARDMMRPLFSRDNVSRLDVLEEHVQTLFRCIE

KEKSPTIAGGT*

SAPVSLAALLLSLTLDSATELFLRQSTSTLQASLDGDDSHLG

MVFHHAFERMVAVLETRV

RLRSFYWLYGTRELRLCVETLHGFVDIAITAS

DMAKKEGSSQALYDFLNVLRERCSGGDEEV

REQVLGLLAAGRDTTASLMSWTWYCLLHNPRVFAKLRSEILSTFGCSPDGPSVTMTFASLKECTYLQHVLSETLRL &

TVVPFNSRRALRDTTLPMGGGPDGKSPIFNPKGTEVNFSTHVLYRR

KDLWGEEDADDFVPERWEKQRGAASAAWHFVPFNGGPR

ICIGQKLALTEAGYVLVRMLQRYDAVKRLDVDLTRDWHNFTVVCSPGSPVNRNQAVLCQLKLA*

 

>CYP585B1 Magnaporthe grisea MG08947.4  42% to 52F5 AACU01001732 cont2.1680

MAHSTVSLALAAVGIFTLYLISKVILKTWQNATEARRLGCKPPASFPSWC

PFGLDIVRKLIISRRQMRTPEWLREQLAKTSTPANPNNLTCSFQIVHTSG

ILTADEVNVHAMLSKQFDDFEVGQTRREIARPLVGESIFTTDGHAWKMLR

GLVRPSFARHQVGDLELDEKHFKNMMACLETGSDGWTGNIDLQTLMFRYS

LDSSTEFLCGASVGSQLKELQLQNGKVTDDEDEIDFASALDRAMEGLATR

GQFFKCGPYIWPRGFEKACRDCHAFIDKYVARRQNKTLAAAEKQPPAESP

PAAKEKYIFLDALAESFPDPVELRHQILGVLFAGRDTTASLISWVFYNLA

RDPARCHKLRRAVLEHFGGPREEEEEDPGRAVTFESLKECRYLQHVINET

ARLCPVVPVNSRRARRNTTLPRGGGPDGQSKVFVKEGTLVYFAPYIMGRR

KELWGEDAERFVPERWEGKRHGWEFIPFSGGPRICPGQQSALTKTAYVIV

RLLQRFDRIESWDPEVDVKHDATATMCSGTGVKVRLHADGF*

 

>CYP586A1 MG09179.4  poor match AACU01000638 cont2.1755

MLNSIPNVYALGLAAIFLAAVYIYRDVRWRRFSQHADLAQLKPSLFWGHM

KALGERLREKPAGTHHDYVFAEIQQELGNPPMFNFDMRPLARPMVVIANH

ELAEQASRASKTFPWSTHKSPSLHEISPLTGENSLLAKEGEEWKALRRRF

NPGFAPKHLLSLLPCILDKTQYFIDHLDRLARSGDEFLFNELTTNLTFDI

IGAVVLDVDLDAQRGKSELVNAFQAMTKTFLDPSPPGFARFFLRQRFIRW

RLRQRMETVLRRYIIDCHEKQNQAQGSIGQSRSVLSLSLEGLETLDESLI

SMISDQMKTFLFAGHDTTSIALQWAMYELARTPRALAAIRAELDEIFGPD

TNPATVRDQLLGPSREELYARLQYTSAVMKETLRLHPPAGSGRFAPKGSN

FRFTMPDGQGMSPVMDNKILYLCHYLIQRDPAVYGETASVFEPERWLGNT

DTSMAGGGDLNDAAPGLAGEKHAIPASAWRPFERGQRNCIGQELANIEMR

VILACTVRRFDFEKVGLGELLRDEKGNFVMDTYGQYKTVNLLYNASKITA

QPTDGCRMRVRLSDKAQAASRT*

 

>CYP586B1 Nectria haematococca

e_gw1.24.71.1

Necha1/scaffold_24:469714-471122

47% to XM_743428.1  Aspergillus fumigatus 46% to 586A1

16 amino acids not found that would match 586A1, no intron boundary here

MVLKPFIFAAVSLLITYLIAKLRFKRFKQYAHLPQLPPSLIWGHML (0)

XXXXXXXXXXXXXXXX

DLIFAEMYEKLGRPPLIFVD

MRPVSNPVALITSHDVAEQVAKSSALFPWSTPKTPTMKDIIHLLGPSSLISAE (0)

GEDWRKLRKRFAPGFAP

NQLMTLLPCILDKTEPFINRLEDHARSGEQFSLLTLIVNLTIDVISAVAMDEDLEAQTIDPSGQSQVIRL

FMELLSTYSSRHNLPWWLAPSLGAKRRRLAGQVDGMLRDIVQRKFEEHQKTSGGNKPRNILSLSFQDIDE

LTPELLSHTSDQMKSFFFAGHDTASILLSWTFYELSRSPRVQKAVRAELDELFGPDPDPSVVRSNLYANG

EDLVRRMTYVSAVIKEVLRIHPPAGTGRYAEPGSGFTVRTSTGEELCLDGTIIYNCQHIIHRDPTVYGTS

ANDFVPERWLGETSAWGFIPSAWRPFERGPRNCIGQELANIEARVIIAIVARHFDFVKVGLGELELDENG

RPILNDKDQYKVKSELYPVSQARRTVRGSSSS*

 

>CYP586C1 Aspergillus fumigatus

XM_743428.1 end may be wrong

AAHF01000010 revised

MFLILSVIATLQLLAFWLLNKLDYYRIKQFAAWPQPKPSRIWGHMKDLNAFISRGEPNRHIGK (2)

EDYVFAEIKAHLGNPPLYLLDARP

MQHPLGIVCSHEIAEQVSRSSKQFPYGLPKTPTLQDYKYLVGPHSILTSE    

GEEWKSLRKRFNPGFAPQHILTLLPRILDKTVPFFETLDRYAESGEEFSLAETCTNLTFDII (1?)

GAVTMDEDLGAQL

PLDKQSEMVRLYHELTASYHQKSSVRAKPSNPLVIWQQYKLARKLNAIIKNHIKRKFA

ELKEASSDEKKSRSVLALSLQGIEKLDAHVLDQTCDQLKTFLFAGHDTTSIVLQWAFY

ELSRTPRVLESISRELDDVFGPDPSPAVVRDKLLAPNGGELLSKLPYTTAVIKEILRV

YPSSGTGRLVPEGSDVYIQLPDGRSLCIDGVLMYNCETLIHRDETVYGESKDDFLPER

WLGDSGADRIPSSAWRPFERGPRSCIGQGLATIEAQVILACAVRQYEFVKIGLGEVAR

DNAGEPILGPQGQYEVKSKLFNV (1)

MTAKPVDGMIMKVKLSSGGRKEL*

 

>CYP586C1   Neosartorya fischeri NRRL 181

           DS027697

           join(701574..701711,701768..701954,702008..702981,

           703037..703117)

           92% to 586C1 = ORTHOLOG 51% to 586B1, 48% to 586A1

           NAME REVISED FROM CYP586C2

           NFIA_003100

MFLILSVIAALPLLAFWLLNKLDYYRTKQFAAWPQPKPSRVWGHMKDLNTFISRGEPKRHIGK (2)

EDHVFAEIKAHLGNPPLYLLDARPIQHP

MGIVCSHEVAEQVARSSKQFPYGMPKTPTLQAYKYLLGPHSILTSE

GEEWKSLRKRFNPGFAPQHLLTLLPCILDKTVPFFETLDRYAQSGEefslaetctn

LtfDII

GAVTMDEDLGAQLPLDKQSEMVRLYHELAASYYRKSSVRAKALNPLLIWQQY

TLARTLNAIIKNHIKRKFAELKEASTDEKKSRSVLALSLQGIEHLDAHVLDQTCDQLK

TFLFAGHDTTSILLQWAFYELSRTPRVLESIRRELDDVFGPDPSPAVVRDKLLAPNGG

ELLSKLQYTTAVIKEILRVYPSSGTGRLVPEGSDAYIQLPDGRSLCIDGILMYNCETL

IHRDETVYGESKDDFFPERWLGDAGADRIPSSAWRPFERGPRSCIGQGLVTIEAQVIL

ACAVRRYEFVKIGLGEVARDSAGEPILDPQGQYEVKSKLFNTMQVTAKPVDGMIMKVK

LSSGGRKEL

 

>CYP586C3 Aspergillus niger

e_gw1.3.1357.1|Aspni1

63% to CYP586C2

MFAQTILLILLPLVVSYFITRIRYSRFKKYADWPQLPTSLVWGHMKALHEFIVRGEEKRHIDIVFGEIQEHLGNPPLMFF

DLRPVQFRLCVVGSHEIAEQVSRSTKSFPYSMQKSPTMGALEPLLGPYSIITQEGEHWKGLRKRFNPGFAPQHLITLLPC

ILDKAIQFTDILDAYARSGEELSLDERCVSLTFDIIGAVTMDKDFHAQLDRSKQDELVRLYRELTTSYRSGSGIQWGWLD

VRVLWRRRVLTRRIDHLISEHIKTKFDELKSKSTEKRSRSVLSLSLQDIDQLNTRVIQETCHQLKSFLFAGHDTTSIVLQ

WSFYVLSRTPRVLDKLRRELDDLFGPDPSPAAVQEKLLAPGGDDLLKRMSYTSAVIKEILRLYPPSGSARYMPPGTGFTV

QLPDGKTLCLDGVVVYNNSTLVHRDEKVYGPTKDEFIPERWLGDADTGLGLSTDNVEDKGNGPVATACIPASAWRPFERG

PRNCIGQELANLEARVILACTVRRFDFEKVGVGAIARDATGAPILDAKGQYEVESELFNTMQVTAKPIDGTVVKVHLAER

AS*

 

>CYP586C4 Neosartorya fischeri

56% TO CYP586C2 Neosartorya fischeri

NFIA_106770 revised

Note: this seq does not have an ortholog in A. fumigatus

MCVVCSHEVAEQISRQSRMFPYSVPKSPTTKAFVDLIGPKSIITAE (0)

GCEWRDLRARLNPGFSAGNLMTLLPCMVEKTLLFVDILDQYAASGEHFMLNELCTNLTFDII (1)

GAVTMGVNLCAQLGEDRQSEIVKIFKEIGSTYSSQRGIWTAPLVRLRRRKLVSKLNALLTGFISETFEEVKRTPQTKESTRLGS

RSILTLMLQDANELTPAIMDRTCDQLKSFLFAGHDTTSILLQWALYELSRTPRAMDAVRRELDSIFGPDTSPRSVSNILL

SEDGTALLSKLRYTSAVIKETLRLYPPSGSARYAERGSRLTLRLPTGRVYCVDGLVLYNCATIIQRDETVYGESKDEFVP

ERWLQDNELNMHLSQYRDHDCYREARFAGPASAWRPFERGPRNCIGQELAMIEARLILASVVRRYDFSKVGLGEIAIDEN

GQPALNSHGQYETKSKLYNVITSLPPRPAFAFCWLTSSVKDVTGDSKTHRWDENEREAKRNSV

 

>CYP586C5 Aspergillus terreus

62% to CYP586C3 Aspergillus niger

ATEG_07654.1

MFLRMVLLAVLPLLGFYLYEKARHSRFQQFAAWPQLKPSLVWGHLKAFNKFIATADPSRHIDIVLQEIRRHLGNPPLFLL

DLRPIQRPLVIVCSHEVAEQVSKSTKELPYSVPKSPTIKALEPLLGPNSILSAHGEQWRSLRKRFNPGFAPQHLMSLMPC

ILDKAWRFLENLDRHALDRNEFSLDEPCVNLTFDIIGAVTMDEDFCAQLGQDKQTELVRRFRELTLNFRTVTSNQWFTLN

PRTIIHRWWLSRRIDTILSDHIRAKFAELKAQSAAGAKSRSVLSLSLQNLDRLDNRIVAQTRDQLKSFLFAGHDTTSILL

QWAFYELSRTPRALAATRAELDDIFGADSSPAVVRDMLLSSQREGLVRRMSYTSAVIKETLRLYPPSGSARHLPYGSGFT

VALPGGGSLCLDGTVIYNCSTVIQRDEGVYGPTAAEFRPERWLGDTDTSMQTNVDAKEDAGRIPASAWRPFERGPRNCIG

QELANLEARVILACTVRRYDFEKVGLGAITHDSTGLPVVASNGQYEVENEVFNTMQVTAKPVDGMKVKVRLVPGAAPLLA

 

>CYP586C6P Aspergillus clavatus

~50% to CYP586C3

EDWKRLRQPFNSGFAPQHLMSLLLCVL

IGLLAMDDDFHA*CEASQQSEIYKALYTS

IK*KFLELQDGSTEKKKSRSILSGSVQDIESLDDQLIEKISASLKSFYFIGYQKTSTVL*

WVFYSLSCTPHALRAVQKILDGIFGLHRQYAVKTELFNISHHE

 

>CYP586D1 Aspergillus niger

estExt_Genewise1.C_70085|Aspni1

44% to 586B1

MLLLLLLTTIFLTSVWICRWIWYERFIKFAQFPQLPPSLLWGHLRIYHEVMQSGSLDRDHDHIFSQIHESLGRPPIVLFD

YRPICRPVLVIANHEVGEQISRSSKQFRSSVPKLGLSFLEPVIGHTSILSAEGEKWKALRKTFNPWFSLQHLMSLMPSIL

GKIEIFVTQLDALSVSGDEVSLVTLTTNLMYDIIGNAIFDIDMDAQHLEPGHQGELVRAFNDLINAYWDDKIHLPWFFTP

VNALKRRRLGNRVHRIMKSIVRRKHEEHLWRQQQQRRTNGYEKRPQPQLPPSITLLSLQETTHTGGILTPELLDQTCDQL

RTFLLGGHDSPSATLAWAFYELSRTPRAQAAVHAELDGLFGPGRDAAAVRTALASPTEGPDLLRRMVYVEAVLKETLRLH

PPASTARYSPKGSGFVVRLPTTGEELCLDDLILYNCSAIMHHDRGVFGSTANQFMPERWLMAEDEKNTNQRTIPPSAWRP

YERGPRACIGQEFARITMRVVLAVVARQYEFIKVGIGALARDASTGLPILRPDGRYEVDSQVYTTRQITARPVDGLKVKVK

 

>CYP586E1 Histoplasma capsulatum G217B

ABBT01000222.1 Ajellomyces capsulatus G217B

45% to 586C3

HCB06858.1

MSVSPFQTASPLLIICAASVPLLVFFLYSRLKYYRFHQFSHFPQAPPDLFFGHLKIVNETIAEEPGRHHGMIHL (0)

VSKASAMFKYSVPKSPTAGMLIPIVGKKSIILTNDETWKHGRKKFNAAFASSNLVTFMPKILDKTMEFLALLDGYVESGEEFCLAE

PCIQVTFDIIGLAVLNIDFKSMQGQDKVSVIVRAFRSLLSTFPRTFAFDNWFTNPKGHYRRIALVKTLTASLTPIVKEKF

DMAHNNIRLDNKVVDRSVMAMGLRQVHTLTPQVLEECVDAVKSFLFAGHDTTAVLLQWSIYELSRTPRALAAMRKELDEV

FGLDADPKSVAKQLLEDSNKLQNLQYTSAVIKESLRLYPPAGSARMVAPGSDFRLNTKDGPLDVGGMVLYVCHYAVHRDP

TVYGETADVWIPERWLGQTSTTSEDYAPSSVDKDKNSSTAGAEGQKIPISAWRAFERGPRNCIGQELANLEARAILAMVA

RRYDFTKIGIGSLALKDGKPVLNQHGQFEVTKPLFGAKNVDLGHLAYSGSSSDREGRMSARSVVYQVDSKVPINDV

 

>CYP587A1 Magnaporthe grisea MG10009.4 AACU01001316 cont2.1914

MTFCRRLQQAIAQFEVDYKLVRLGLVATAALIGIAVVYYAFVGYRVRSRFQRLQKQGV(0)

PMMPHNPILGHLPLMPKILAGLPADAATLFLPLQIQENWRSVFPES

VTELPPVVYLDLWPLIPPTVMSFSGDVSAQFTQEFNQVKLPDQKRFLMPL

TGTRDVSSAEGDEWVRRRKALNPLFSPQNIMAWVPELIVELEVFLEGLRS

RAGKEGGWGPMFRLQRPVQLFTLDMINHFCLSKRFHEQTTTMSVVSTGVL

DILSRQAMFLHIGNVWGYYSPWRLFKVWQQQQLMDDFFLPYVRRRFDTRA

KEDKASPSGNDRIDLPHVLFKAAEANSAKEDRKLQYVDVVRDVMGEAKLM

VFAGHDTTTFALTSAIKLLSEHPAALEKLRAEVEEVLGDRPADRIKEEPH

LLNRLVYTNAVIKETLRVHPNVGTLRIGAEGFSLYGPPGSGQEGVAFPVG

GYLLWDATSAIMRHEAVWPRAREFVPERWLVEQGHELHPGEYQKNWYRPF

EQGPRNCVGQPLAMAELKMAVACVVAEFDVECDWDEWDVVCGNVGPAAKK

FTHNGNRLYMVNEEGPPKFKDGMPVHVRLRSGKQ* 3789+

 

>CYP587B1 Fusarium graminearum FG04249.1 AACM01000185 FGcontig1.185_scaffold2

MLPLEYQHDGSSFLLHCLSFSGVVLAALLIHALRKGYQVRKKFKALEAQGI

PIMKHSMIFGHLESVGKLVSSLPPDAHGDYLMMMIRENWRELFPQCTKCPPVAYIDTWLF

TAPMVISLNLDVSTQFTTQHSLPKAYEQKHTLYQLTKNRDLASMEGEEWKIWRKRLNPAF

SIQNINSRIIDILEEVEDFAAVLKSKAGDDGQWGDVFLFEKATTNLALDVIVRFFL

DVRLNEQWGANSPMCHALQDTISRLCFFVHIANFIQYYNPWRQFX

LWKNYKTLADSMTPMIKERTRARQEDPTAKGKTLVDLVVQALQEEKAEQS

NTANEGKTQPGLEFDTEFMDMAIGQMNTFLFAGFDTTAATVAWLFRLLCQYPDVVAKLHD

EHDAVLGTNAWAAANVIREDPQLMNQLPYTLAILEESMRYHTNVGTMRRGEPGFFLVGPP

GSDPGFDGKKLPTEGFIVWDGTWAVHRDPEIWHRPDEFLPERFIITDREDPLFPPINGWR

SFVSGPRNCIGQHLLVLEVKLVMALVTRYFDVEVTWEKWDRAK

GTTNAKKAPTIWGDRCYQVGANALPRVKDSMPVHVRVRA*

 

>CYP587B2 Nectria haematococca

e_gw1.18.184.1

Necha1/scaffold_18:122860-124804

53% to 587B1 , 41% to 587A1

note: this gene is longer than most other P450s and it may have added

loops in the structure.  These appear to be conserved with

orthologs in other species.

MWLLTFRDDSTLTAAQVLSASIILLAGFLLRCINRGYQIRKRFQALKAQGI (0)

PIIEHSIIFGHLKVVRKLTANLPPDAYPDYLQTQLQDNWRQLFPQ

CTKCPPVVYIDTWPLANPLIVSLNADVSSQFMQQHSMPRFRDAK

DFFHPLTKNRDVVSMGEAEWKIWRKRLNVGFGAQYITSRIPDIVEEVEQFVDVLKSRAGTGETWGPVFLL

EHATRNLTLDISVRFFL (2)

NMRLHEQRGNPSPLAVALEDTLSKMMFRVNILNVVPYYNPWRHFKLWWNYRTL

TQQLIPPMQRRLSELQADNTIPRKTLIDLIVQALEEEAAEEQGSSGGKPKDLIGLANDSLEVVVGQLNVF

MLAGFETTGSAISWVFRLLCQHPAVLAQLRQEHDEILGTDPWGAADTLKENPQLVNMLTYTHAVVRESMR

VYTNVGTVRQGDHAFFLTGPPGSGPGFEGKKLPTDDFGLWDGVSAIHRDPKIWHRADEFIPERFLVTDTN

DPLYPPPNAWRAFAAGPRNCVGQHLAMVEIKLVMAMVVRCFDIDCRWDEWDRSR(2)

GNKDTKTDHPTVWGDRYYQVGRYGPPRVKEGMPVHVRVRSQI*

 

>CYP587B3 Gibberella moniliformis, Fusarium verticillioides

AAIM01002228.1

FVEG_10439

4962  MWLLGLQDDGASLLTRLLRFSGVILVALLVHAFRKGYRVRKKFQALKDQGI  (0) 5114

5167  PIMKHSMILGHLEVIGKLVSTLPSDAHGDYLMLKIQENWRELFPQCTKCPPVAYIDTWPF  5346

5347  TAPMVISLNAHVSAQFTQEHSLPKAYEQKHVLYPLTKNRDLASMEGPEWKVWRKRLSPAF  5526

5527  SIQNINSRLPDILEEVEDFANVLKSKAGKNGEWGEVFPLGDATANLTLDVIIRLFL  (2)5694

5762  NIRFHEQWGKSSPVCAALLDTISRMYFFVNPANFIQYCNPWRHFKLWQNYRTLVKSFTPV  5941

5942  LQERMNKLQQDRTAKGKTLVDLIVQALDAERAEQKGAGQDDKDQLNMELDADFLEMAIG  6118

6119  QINTFLFAGFDTSAATIAWLFRLLSEYPDVLAKLREEHDAVLGPNAWGVAGVIRENPQLL  6298

6299  NQLPYTLAVLRESMRYHTNVGSMRRGEPGFFLVGPPGSDPGFEGKKLPTEDFIVWDGTYA  6478

6479  IHRDPEIWHRAWEFLPERFLVTDPEDPLYPPPNGWRSFEAGPRVCIGQHLATLEMKLAMV  6658

6659  LVARCFDIECAWEEWDQIN(2) 6715

6779  GTTNSEKARPTVWDDRCYQVGTDSPPRVKDGMPVHVRTRGL*  6904

 

>CYP587B3 Fusarium oxysporum (temp 587B5)

94% to CYP587B3 Gibberella moniliformis

FOXG_11378

MWLLGLQDDGSSLLTRFVRFSGVILVALLVHAFRKGYRVRKKFQALKDQGIPIMKHSMILGHLEVIGKLVSTLPPDAHGD

YLMLKIQENWRELFPQCTKCPPVAYIDTWPFTTPMVISLNANVSAQFTQEHSLPKAYEQKHVLYPLTKNRDLASMEGAEW

KVWRKRLSPAFSIQNINSRLPDILEEVEDFANVLKSKAGKDGEWGEVFPLGDATANLTLDVIIRLFL (2)

NIRIHEQWGRSSPVCAALLDSISRLYFFVNPANF

IQYYNPWRHFKLWQDYRTLVKSFTPALQERMNKLQQDRTAKGKTLVDLIVQALDAERAEQKGVGQDSKDQSNMELDADFL

EMAIGQINTFLFAGFDITAATIAWLFRLLSEYPKVLAKLREEHDAVLGSNAWGVADVIRENPQLLNQLPYTLAVLRESMR

YHTNVGSMRRGEPGFFLVGPPGSDPGFEGKKLPTEDFIVWDGSYAIHRDPEIWHRAWEFLPERFLVTDPQDPLYPPPNGW

RSFEAGPRVCIGQHLATVEIKLAMVLVARCFDVECAWEEWDRINGTTNSEKARPTVWGDRCYQVGTDSPPRVKNGITSVT

KLYLSSYILELIQDTAWWHCSSFG*

 

>CYP587B4 Aspergillus terreus

AAJN01000219.1

ATEG_08901.1

5121  MGLLSNFQNHDSSYLLLQSVTALLVTASAYGLYRGYQVRSMFQSLKKQGI  (0) 5002

4919  PLMPHSMIFGHLEVIGKLMSRIPSDAHGDYMMVLIQENWKELFPGCSKCPPVLYIDLWPF  4740

4739  APPMLISINPDTSAQCTQDYSLPKAYQQKRTLYPLTRNRDVSSMEGAEWKVWRKRLNPGF  4560

4559  SIQNITSRISDILEEVEVFVGVLEARAGNDGAWGEVFPLENATTNLAMDVILRFFL  (2)4392

4329  NKRFHQQTGPRSPITVALLDTISRMYFYVNIFNFLSYISPWRHFKLWLNYRTLFNSLSPF  4150

4149  LTERINEIQKNSQVTNKTLIDLLVKAMDEEKGTNGAAGDLSTNTDFLETAIGQI  3988

3987  TTFLFAGHDTTASTICWLFRLLEQHPDILSKLRAEHDAILGPDPSQAAAALRANPQLLNA  3808

3807  LTYTNAVLKESTRVHTNVGTMRRGEPGFFLTGPPGSGPECEGKQMPTDGFVVWDGNFAIH  3628

3627  RSPDVWPRPNKFIPERFLVTDENDPLHPPKNAWRFFELGPRGCIGQHLAMVEIKLVMALV  3448

3447  VRRFDIECAWDEWDSKR (2) 3397

3356  NFKGPQNMVWGDRCYQVGGDSPPHVKDGMPVHVRVRG*  3230

 

>CYP588A1 # name changed to CYP534B1

no sequence

 

>CYP588B1 # name changed to CYP534C1

no sequence

 

>CYP589A1 Magnaporthe grisea MG08234.b 2 fused P450s poor match to second half

AACU01000307 cont2.1540

MEKGRVRISGDVTLS

PRDKDSQLVKIISTKPTMIVTIPVLLATYILYWTVRLVKNILRANATGLP

VRWTPVAPTNPFWIVLGKPTSRVLRAILPSFLSNWTYFSTFDWHWLDRLY

LDFEVHEKNGDTFLHATPSGLMLHTRDPQVAQQILHTCPKPDDVAKSLEL

CGPNLVSSSEVDWPRHRKVTVSTLSDRNNRLVWSETVRQTLNVLQSYQPG

HGEIIDPEGDIRRIYLNMFSAVCFGRPLDAEKEPGETTDGKLPGGHKRSY

RYCLETSLRDIFILHTVPSWIKYLPPALVPHKISEFFAASREMMQYLDEM

IRRCQDEAQEGGEESKSGDNLLRITVRSGLQSNTKEPQGRSYLSKEEVRG

NLLIYSLAGHESNAHTLTYALYLLAACPDVQDWLAEELAAITLLDGQLSA

EETYFGIMAKLPRTLAVMYETLRLYPSIIFLPKTTASRSVSIDLGHVEGQ

SKAIIPPNTNIWVNMCGMQVLPEVWGDDARTFRPSRWINSSGQLAAPSLV

AGSAYVAWGGGKRVCPGQRFSQAAFAASVVALLTGGKRVHVVPREGEEEG

MARRRVLEVLDDSFVGTTIHMRRAGDVKLVVA*

 

>CYP590A1 Magnaporthe grisea MG08795.4  poor match AACU01001208 cont2.1657

MGLFTFGIFLLVSVWISSHLKSLARNYSAAKALGLPLIIHPFDPDSVTFA

LLSEPLHAILVRILPSSAYAYIQPCFFGWEFQNKGAIHQRLGPAFVLVTT

GVNRLCCSDPTLASTFMTRRRDFVHADVSNHVMGIHGPNIISAGEDAWSR

QRRIVAPALNERISSDVWDESIEQASSLADFLVSSSSTPADDGASDTVPG

LRSVAINVLSRIAYGSVKTFNVWNPTYDPKADLSYVDGIALCTDLLVAAA

FIPAWILRLPVMPLSLQTLAAALRKLPDLAKNMLEQERERISSSPADEAA

TTTDTKKRRDTIMTTLLRLSDQAKEEAASGENKPTAEDGVSSVGSKAYLT

EPEIQGNLFVFTAAGFDTTANTMAYAVALLAAHPQWQPWIQAEIDSVVGS

ITCGQDGRLQVPEYGETFPKLIRCLAVMFETLRLYPAVSLLLRKTTVDQT

ITLPGPPLIQVPLKAPFTVQVNAMALHTDPEVWGSDALEFNPGRWVRPDP

ETPGGHVLMTPPKGYFVPWSIGPRTCPGQKMAQVEFVAVVMTLFGRCSAE

PIPGDGESSELARRRVLDVTEDSQPGLTLQMKKPRDIRLRWTRR*

 

>CYP590A2 Phaeosphaeria nodorum SN15

AAGI01000245.1

52% to 590A1 Magnaporthe grisea

128907  MGLLLIGIVAILLIWIMSHLRIFIRNYNAARATGLPIIICPYDPDS  (0)

        VVFTFISEPLRPLLKPILPASIFAVFDATCWGWEFHDK  129266

129267  GAAHEKFGDAFIMVTTGHNRLVCADPAMSQSVLARRKDFHHPELSKKTMGVLGPNLVT  (0) 129440

        SRDESWSRQRRIIAPTFNERISLDV  129626

129627  WKEGVQQASALVDHVLSSSTLSQSMTLSTSPAADKSSDSLPGLRAIAINVLTRVAYGRHT  129806

129807  PFSIASSYRDATKALTYVDGIALVVDLLLVAAFIPAGILRLPFMPQLSKKLGQAMVKLPD  129986

129987  LTVDMLDQERKRISSADSKEFQNTIMTTLVRLSDQGREQDDDQGSSGK  130130

130131  SVATGGNKQYLTEEEIAGNLFIFTAAGFDTTSNTMSYAICLLAAYPEWQAWIQAEIDVVL  130310

130311  GAGDHEQISLEDYTAIFPKLTRCLAVMFETLRVYPAVSLLIRTVETNQTIFPSSSKS  130481

130482  DSFILQAPCAVHVNIMALHTSRSTWGADTLDFKPARWFPEGQDSMYVPPRGTFMP  130646

130647  WSSGPRNCPGQKMSQVEFVSVVMTLFGKCSVEPVAEKGQTVKQARERLLGLLQDSQPVLT  130826

130827  LQINKPREVKLKWTRR*  130877

 

>CYP591A1 Magnaporthe grisea MG03811.4  poor match AACU01001028 cont2.745

MFSTAAIALSSTLAAWLLYRALLQKLYPSVLPGVPHNAVHAKHLLGDLPA

IDEYVSKNGCTKSEATFDTINRGTTNFPIAQMLTPNIMPHAILTVDDPRE

VEDILRRRAKEFDRSSMTAAFFKSLLPKATISLFTTPELKVQKKLWADSI

TSDFVKNVVAPHAYRAANRLVELWRLKAGSEFQAQPDLIDAMMDLMWATV

LGSDLGVTSSKLAATAAGIRAQDPGQLEKAASTSREMFFNVTSFLEGESS

YIRAGLEPLRTRVIQFLPRYHRVRKSMNQEMGNLISMARGRRGHERDGTE

GGVACAMDLVLERELQLAEKGNSVAATNLEQELMLFMIAGIDSTAFTLAW

CVKLLTKHQQVQQELRDGLVEAFGVGDNAQALPSPQDMASRDIPYLDAFI

HETLRMANTAGSVVRRATTNTVVLGCSIPAGTELVLNTRVLKTPIPVDES

LRSATCREAQAKRPRGGIEGPSGHNLEAFNPRRWLHPQPEGRDVFDPEAL

PTLVFSHGTRGCF

20908 GKRLAMLDLRVFIIVLVLEFRFMPIPENRNDWSAEEGIFRSP 20783

LQSPVKLSTYD*

 

>CYP592A1 Magnaporthe grisea MG07488.4  poor match AACU01000906 cont2.1392

MSNITFSGPSFLLGDREASLELTPKILGAALLAALLLLPVLWHSKTAPPG

TKPVPGPKGLPLIGNLLDIPKKHAWLKYREYIEEHGPIVRIKLMGQEHIL

LGSEAVVDDLLKSRGGIYSGRPQTQACSILTQDQHVLMMPAGEPLRVRRR

FLSQLLTRGAVSQYEPYQWLEAYRLVVEMVRDPSDYQGLIEHFSVAMGSR

ILFGQAMPDRSGLQKEVIKIGHTFEAVLTPGAFLVDVLPWMRHLPDLVAP

WKRWLKRMSRRDEAFYYRMWERTKADLDEGRDAPSWARLCLEDMKRAAVG

GKGMGITEHEAVHLIGVTYTAFGTTPANMLSLLLGMTRHPEWWLRMQAEI

DDVVGAERLPTLADLPRLPVLRAVLSEITRLWPVTPDGVPHELTQDDVYK

GYRLKAGSVIHYVTWACGRDPEMYPEPDTFNPGRWIDPSFPTYKEPLTEF

PTLMNTLMFGKGRRQCPGMIVGVRNVYVQTMMLVWACDLGRARDEVTGEE

IVPPLYDYGAGFNVAPNPFKFDLKPRGAGRMRMVEAAYEKALAEDPMKE*

 

>CYP593A1 Magnaporthe grisea MG02292.4  poor match AACU01000444 cont2.449

MFNLPAISVASGLALLYLIVSHLRTRRKPFPPGPPGSLILGNLLQIPTKK

ACLTFQKWSREYGSSSDGLLGLRMGPWARMIVLNKHKQYRDLLEGRSTVY

AGRGSVPIMEKLRVWTKPGEPYVGNFASWQYNDELKRERRATHEYITRSA

QVEKLLPIQDAESTQLMWDLLSFERDKKAGDTAPGSHHLLFFRTFGAVVL

AAVYGIRGKDTTHDSHLGQFESIVKDFIQLLALTAAPPYEVFPFVDYIPE

FINPWRGWILRGKEFRVRWFRFWKEFHDIALNSAESKTSREGDGMLLQLE

GKGYSDTEKLSFGATIMNGGADTTAITCLTMLGAFACFYEAVRWRPLTPL

MTRRVRQDHEYEGFFIPKDTMVLFNIWALSHDTDVFDRPQDFMPSRYLPQ

CRGDPDVKPLPVYGFGTGLRNCPGQKMAENSVMLVVAKLLWAFEISSPDG

KLDTSMETGYDDAFMLAPNPARLSFRLR

 

>CYP593-un1 Magnaporthe grisea MG09380.4  poor match AACU01001225.1 cont2.1796

pseudogene

9124 MTLFTVPMAAILLLLVAAVLLVPRIRSKSRFPPGPPTLPFLGNLHQMPTS

KAFLSYQEWSTTHAASSSGLLGFHLGPSAKLLVLHKWEQVRDLLDLRGAIYSDR

PYVPALD ()

YGPKWRRQRRTIDEFLRDRNAQLAPVQQAEGTQMVWDLLHHGRDCHDYMLRFSAAVILASVYGERGRD ()

deletion of about 165 aa

LRRLLGE (0) 8468

8357 TMRFRPQFPTSIPHATTKDDTYQGYSIPKGT

TVMMNVWALYHDPAEYEDPDSFVPERYLRNRFGTRHDHVESGQDDAKPQQ

GFRRDTWAFGAGRRACPGRRFAENTLLTLTAKALWRLTLWPWARWTWTSG

PGSRMRF* 7941

 

>CYP595A1 Magnaporthe grisea MG09156.4  35% to 68D1 AACU01001649 cont2.1746

MIAELLFNPGTWTALAVLAAAYYHFFLRSPALPFPPANKSASELQDASSL

ITNSLSKSNGGPVVVTLGSGGRKILLPSSLAAWVKTNRDLDHQQLVREDF

MSGIPGFEGNTLLHQSGSPIMDIIKTRMGLNDGTLRTLNASVKRTLHDTL

GEGAEWHAIDWYAGTSNIIARAASSVFVGPEKADDPEWLELAQTYVTAYF

TGVAELGTYPAWARSIVHWFLPNVKTCRKLLPRARVIIGQVAAAREKEAA

AARREGRPAPEYNDALEWVRAVSGGEVDPAEAQLCLAMAAFFTTAELLRV

LLIEVARHPEYVDELREEISTQISKHGLSVAGINGMVLLDSFMKESQRLN

SGPVVLERVALRDTALPDGRIIPRGSHIMVDSMDLWKSEKYPDPNKFDGR

RFLKKRKAGDSASQFVQSGPDYHVFGGGRHICPGRFFASQELKLIMAHLL

LKYDVRLGEGCDPKVIPNGFFVMIDPTVKFEVKRRKSRDADALL*

 

>CYP595B1  Aspergillus oryzae

          GenEMBL BAE58469.1

          46% to 595A1

MFGQIFSSVFAWTLIAILAGLYRFTTKQRPIFPVVNDYRGDFFRRKAYREYNQNAKKLIVDGLAKHGSPI

TLRVPDGLKIVLPSALSEWVKTNRDLDHQELIREEYFAGFPGFEAQDTLHAPDGMLIKLLRTNLSQNEEI

VPTVNRHIGPALQHYWGDSGIWHTIDWEDDTTGIISRAAASIFVGPEKAADDEWQTVVQAYVREYFAAVS

ELHTWRASLRPIVQWFLPHASACRRLLHQSRAIMQEVVRKREREAQAAEDQGLVAPRYNDVLAWTMQVPD

NKHPAGDIQLALAMAALFTTTELFKQILINIARHPELVEPLRKEIKTSLLGHGLGLTALAKMELLDSVMK

ESQRQIPVTVGLERKVIRDTSLPDGTKLPKGSHIMVDATDMWNPEVHVNPEVFDGYRFLKRRHAGDKASQ

FVQSSREHIVFGGGRHICPGRFFAGTELKLCLAHILLKYDIRLKEGYYPQPMVLGVYAIVDPMTQLEVRR

REHTEDLVF

 

>CYP595B1  Aspergillus flavus

99% to CYP595B1  Aspergillus oryzae

AFL2G_01662 revised

MLGQIFSSVFAWTLIAILAGLYRFTTKQRPIFPVVNDYRGDFFRRKAYREYNQNAKKLIVDGLAK

HGSPITLRVPDGLKIVLPSALSEWVKTNRDLDHQELIREEYFAGFPGFEAQDTLHAPDGMLIKLLRTNLSQNEEIVPTVN

RHIGPALQHYWGDSGIWHTIDWEDDTTGIISRAAASIFVGPEKAADDEWQTVVQAYVREYFAAVSELHTWRASLRPIVQW

FLPHASACRRLLHQSRAIMQEVVRKREREAQAAEDQGLVAPRYNDVLAWTMQVPDNKHPAGDIQLALAMAALFTTTELFK

QILINIARHPELVEPLRKEIKTSLLGHGLGLTALAKMELLDSVMKESQRQIPVTVGLERKVIRDTSLPDGTKLPKGSHIM

VDATDMWNPEVHVNPEVFDGYRFLKRRHAGDKASQFVQSSREHIVFGGGRHICPGRFFAGTELKLCLAHILLKYDIRLKE

GYYPQPMVLGVYAIVDPMTQLEVRRREHTEDLVF

 

>CYP595B2 Fusarium oxysporum

55% to CYP595B1

FOXG_08910

MAILFSPLGLGVSLVAILIGAIIFVNKKTKFPIINKYPKDFFHRRANHEYKTNVRKLLKDGAAKHGENPFAILVPNGIKT

ILPPSCVGWAKNNKDLDHQQLVRDEYFASYPGFDVQHVLHHPNRMVINMVQGKLSKTDKTLPVMNEHIKAGLSDIWGEGK

SWKTLNWEDGTTGVISRAAASIFVGPELAADPEWQKVSRAYVLDYFGAVGEMHLWPSWLRWLVVWYLPGASACRAGLKRA

REMVNEVVQKRRQEEQEAKLEGKEAPAYYDALAWTLESPLGNEFEPADVQLALAMAALFTTSELFRQILTEIARRPEVVE

HLRREIEDVAPYHDFTATSLVKMQLLDSFMKETQRQIPSLVILERLVIHDTRLPDGTVLKKGTHIAIDSREMYDPAKFEN

PEEFDAWRFYKRRQAGDNTSLFVQSSPEHAQFGMGKHLCPGRFFAGSELKLCLAQIILNYDIRLKDGCSGKSMQFGFLSA

TDPYTQLEVRRR*

 

>CYP595B2 Fusarium verticillioides

90% to CYP595B2 Fusarium oxysporum

FVEG_06528

MAFLASPFGLGVSIIAILIGAIIFAKKKPKFPIINKYPKDFFHRQAHHE

YKTNTRKLLKEGAAKYGDEPFTILVPNGIKTILPPSCVGWAKNNKDLDHQQ

LVRDEYFASYPGFDVQHVLHHPNRMIINMVQGKLSKTDKTLPVMDEHINV

GLLDIWGECKSWKTLNWEDGTTGVISRAAASIFVGPELAADPEWQKVSRA

YVLDYFGAVGEMHLWPSWLRWLVVWCLPGPAACRAGLKRAREMVNKVVQK

RRQEEEEANKQGKEPPAYCDALAWTLESPLGNEFEPADVQLALAMAALFT

TSELFRQILIEVARRPEVVEHLRREIEDAAPDHDFTSTSLVKMQLLDSVM

KETQRHIPSQVILERLVIRDTRLPDGTLLEKGTHIAIDSREMYDPTKFEN

PEEFDAWRFYKRRQAGDNTSLFVQSSPEHAQFGMGKHLCPGRFFAGSELK

LCLAHIILKYDIRLKDGCLGKPMSFGFLSATDPYTQLEVRRR*

 

>CYP595B3 Aspergillus terreus

72% to CYP595B1  Aspergillus flavus

ATEG_02452.1

MLVELLSNGFTWSLIAGVAGLLLLFTKQRPVFPVVNDYPGDFLRRKAYREYNKNARKLIADGLAKHHGPITLLVPGGMKI

VLPSALSDWVKTNRNLDHQELVREEYFARFPGFEAQYAAHSPDRMLIDMIRTKLSTNETILPTVNQHLATALQEHWGSSG

VWHVINWDKDTTGIISRAAASVFVGPEKAADPEWQTLVQTYVREFFTAVGELHAWPAVLRPVVHWFLPRMSTCRALVRQA

RATMRDVVYKREQEAKAGRERGLEAPQYNDVVAWTQNVPSNNLPAGDIQLALAMAALFTTTEALKQVLIDIAQHPELVDP

LRQEIKQSLSDQGLGLAALGKMELLDSVMKESQRQIPVSVGLERKVIRDTFLPDGTRIPQGSHIMVDSGDMWSAEVHDNP

ESYDGYRFLKRRQAGNKKSQFVQSSREHNTFGGGRHICPGRFFASNELKLCLAHILLKYDVRLKHGYCSRPVQFGVYASV

DPVAELEVRRREVPDDNVL*

 

>CYP595C1 Mycosphaerella fijiensis

47% to CYP595B3   Aspergillus terreus

e_gw1.7.216.1

MEAKRSDWGLDLLHEVRSWIYILALLGTIVYYWRSRSAIFPVVNKYSWDFTRRKAHAAYMQDARGLIARGLREKKGPITL

DTPSGPRIVLPSSLTDFVKNNKDLNHQELVYHDFFGNYPGFEANAVMHNPDNVVIDTIRKKLSNNARLIPIMSEHIGEGL

AGIWGDAESWHALDWVPDTMKLVSRAASCAFAGEHLARDAEWQRLTQTYATNFFSAAFELKRWPHWTRPTLHWFLPGATE

CRKLVKDVSIATQAEIDRRRKEKDAGAETDYPDAIIWAQEIAGDKKINHGAIQLAFAMGALFTTSEALRGVLIDLCEHQE

LIEPLRKEVREALSTSGVTLAALAKMNLIDSVMKESQRLGPPSIVGLERQATKDVYMPDGTRLPRGTILAVDSSDMWNPS

INPDPDVYDGYRYYRKREGGDNQTCFVSSTREHNVFGMGKHICPGRFLAAVEIKLCLAHILLKYDIRLQRGCEPETIKNG

LFQMVDPAAKVEIRRTSGGEAKLL*

 

>CYP596A1 MG10351.4  39% to 68A1 AACU01001708 cont2.1991

MNFSSLLLSVALLGASLFIIGYWFYERVYTEWPWSGFPLAVLPGKGRKPK

ESYMESASRTIAAGLEKYSGPFQMITSTGPKLVLPHKYADEIRNRPELDF

NEAFRKDFFAHYPGFDGFKASLDNPFLIPQTLRLKLTPSMGLVTHHLVDE

ATDALHGIYGEDPNWRSVTFKQNNLDLIARLSSRVFLGKPICRDKQWLEI

AKNHTVDSFLAARDLRAQPFFMRRIMHRFMPHNVNIQRHYADASRIISTE

VENRRRRAEQLLSEGKKPPRVSDAIGWMVEVAKGQKVDYVAAQLSLTVAS

IHATTEALTVALMDIANYPELKKQLREEVIQVIREGGWSKQALYNMKLMD

SFLKESQRFHPPSDVTLNRKVMQNMTLSDGTRLPKGAIIMVASKLRDPKI

YERPNEFDARRFLRLRDDSNNANQVYQNARVHADERGVTKGHGRHACPGR

FLASSELKVALAHMLLKYDWELDETDKMPKFFDNETAHMTSPMVKLMIRR

RKEEINLDLDEGLNLSDIED*

 

>CYP596B1 Nectria haematococca

fgenesh1_pg.scaffold_19000057

Necha1/scaffold_19:141600-144731

43% to 596A1

MLLSTMFDFFQRSPSGETNTLSLYLQTLLLGVVSWLAFFYFSKPKYYSGFPIVSSEAKGTPATRWFLEGYQMV

LRGLKTVSGPFQVMTGTGPILVLPNNYANEVR

NNPHLSFNRFFDKDFFVKYPGFEAYKTGYQDGTFIQEVVRTKLTQSLGLVTDDLVDEMTASAHDLIGEDK

EFKAVTLKEVISLMVARLSSRVFLGKNLARNDRWLHIAMHYTHDSYSAAAELRQWPFFLRPFVYWFLPLC

RKLRQEVKDADALIMPEVNRRKEAVLKAQASGEKPPRTSDTIGWMFEISRGRDVNYVYGQLFLSFAAIHT

TTETITGCVLDISSYPEIVQPLREEIISVIGEHGWAKTSLYKLKLMDSFMKESQRRHYMGM (1)

TSIHRHADEDIKLSDGNVIRKGSRMCVTAEPFIDPKVYPEPEKYDVYRFLNLRSQPGQENAHQFVTTSPQHMLFGHGTH

ACPGRFFASNEMKIALCHLILKYDWKLCDGQTERPKNLQVDAGFLTDPTVKVMVRRREAEIDLDVGIAEE*

 

>CYP596C1 Mgr014 Mycosphaerella graminicola

47% to CYP596B1   Nectria haematococca, 43% to 596A1 N-term from JGI model e_gw1.7.728.1|Mycgr3

MANDLITYLLDVRPFSSFLLIGIGIITLVFARWALIRPKPVPGIPVVLAAPKGWRGLGEQNAFIQHARTILKQGLRECTGCF

QVWTSSGYKVVVPNRFADELKSHPDLSFNEAFQEPFFHLPGFEDQYQALQNDWFIQDVVRIKLTQSLALVTDALVEEAIFSSHSYLGDSMSWSTIRPKFFVLDAVSRLSSVVFLGKKLARDKSWLQIAKDHTVDSFAAQDILRAVHPLLRWPTYYFDLTCVRLRQQVKTSRKLIAPEIEARRKRAVEAQKEGRKFTKESDAISWMVDIAKDMPVDYTAAQLALTTAAVHTTSETIANCILQLCEAPEIVPALRAEIIDVLNERGWSKTSLPRLKLMDSFMKEVQRTHAMAMTSMKRKVKKSITLSDGTHLPAGITIMISDDKTHDGEIYSNPAEFDAERFLQLRQQPGEENRHQFVTTTAEHLSFGHGQHACPGRFFASNEIKIFLCVFLLSYDCQFVPGEKRKNALEFETSMMVDPTTEVQVRKRTPEIDL

 

>CYP596D1 Mycosphaerella fijiensis

42% to CYP596C1 Mycosphaerella graminicola

41% to CYP596B1 Nectria haematococca

gw1.2.702.1

MTSIILAQFPSSLNYVLCLTCIGLSITLIIYFSRHHSPKPVPNIPILSHD

RDEYFRRGYDLVKEGQEKFPGCFQLRTATGWKILVPIRFIDELRNNRFLSFAKGNDKDAFIDYPGFEAMEAACHDDYFMQ

EVVRVKLTQSLNLLYSSVIDESAATMSEVLGESKEWRTCRIKDLVSRIVARVTTRVFVGFPLCRNQEWLDLVVNHTAAVF

LAQKRMRQTPPALRPLVHWFLPETRRLRRELRAARALITPEIARRRAAVEEALQHGKIPKESADAISWMVEVAQAQGRKS

DVAVHLVSLSMTAIQTTSEVMTNCILQLCETPSVVADLRAEIIPLLREGGWTKHTLYKMRLLDSFMKEVMRHHDFLRVTS

WRGCTEEVTLSDGTVFPKGSCIYFDDSNVVNPEYYPDPEKFDATRSLKKREQPGQEERHQFVSLQTDHMPFGYGTHACPG

RFFANMELKVMLCNFLLKYDVRLVPGEEHRPADILFEVQRMVPPDCRAQIKVRDVEPEVDLYSPESSI*

 

>CYP597A1 Magnaporthe grisea MG01700.4  36% to 68D1 less to other 68s AACU01000096 cont2.318

MTQWELVFGMLAEWQLEILVTITVLIAGSLLYLDRPAVYPGIPLMGAGKG

WFGYRQAVNKYRVNGREMVREYSKTNKPFQVITDTTTKIFLPAEYTEEVK

GIKELSFGKGLSIELFGGYRGFEAVAALGDDESNIIQTVSRLKITTSLKY

LTDEISDEASAALQEQLGDNIQEWQEASVKPIVRNLNARLSARVFVGPEL

CRNKAWLDVSTSYAAQCGVAAFKLQAWPRVLQWPVSLFLPECRVLRRMAA

DARRILAPIASNRSDKPVDPKHRGGDTILWIQEAVSKAKMSHKYSVADFQ

LGLSLAAIHTTTELLTNALFDIVTTGPELIDELRREIIQVFNGEGGIASY

NEEEVFTKERLYKLRLLDSTLKETQRLRMSRLGGMPRVATAPITFKSGLA

IPAGAFTHVLLTAHMDPSIYWSPETYDPRRFLRLRSQPGQDKNWQMVTTS

PHHLAFGYGEHACPGRFLAAAQVKIALVRLLMEYDWRFDGGRPESLDVGG

GMNWANPNAKVSFRKRKAEVAF*

 

>CYP598A1 Magnaporthe grisea MG10345.4  poor match AACU01001708 cont2.1991

MELPDFVWTWGPLLATFGILLAWNAVSLRRSFQTTSNYPIINYQKRSWTY

AAARRQFVHNARQLLVEGSQKFSGPFKVITLSKPLIILPPEYIDAINAER

KLSFLEYVREEFLSDFDTFRTCRPMPPGMFSKAIQNGINRRLPKFTQGMS

SELSACLDKSLGYQQGWHEVNFINEARGWVAHMTTLVFLGESFAHSQQWI

DVTTSFVENMFMAMAITRSFHPLLRPLVDRFSPLNRKVRKYQEVIASILG

PVIQERHMQIKVAEMKGQKPDLPDDSIEWFRQAADGFNYPEIWIQMGLNQ

VSMQTTSNQLCQSILYLCANRQYFEPLRQEAIEVLQRDGLEKAVTNMHSL

TFMDSFLKEAQRLKPEGLASMHRRAMEDVQLGPDVTIRKGEHLAISAHQM

WQESNYPNPEHFDPYRFIERRKNPKYANRWAFVSTSPDHMGFSHGKLACP

GRFLASSVLKIAMLHLILKYDLRIADPKEATPWFHGTYAMVNTNARIWVR

SRKPEVDLESLARQIQHE*

 

>CYP598B1 Mgr069 Mycosphaerella graminicola

55% to Mgr073, 44% to 598A1, 39% to 68L2 N-term from JGI model e_gw1.9.207.1|Mycgr3

MTTIELLRGAVNTIVGPERIIPWPATLAIAAVGWWILSSWLRPV

SKFPTINDPKENWTSFPAKKAYLKNCQHLLKEGARKFSGPFNLTTDNGTTMLIFSPEYIDAINEQESLSFPEYAIRNLNL

KGYKTFRTFGPQPDHMFYEAVIKGLTRNLAKFTKPLSLEMDACLLSSWGESTEWHSVEAKEDVLDFVARLSSLIFLGPDF

VHNKEWIRIAKNYTVDTFTAIAICRYIPALIRPLFVQFLPLCRKVRRDYTTCANILAPVLASREAELKAAKSESRPPNLP

DDAIEWFRNAAHGREYNQTDVQVSLQVASIHTTTDLLAQAILNLCAHPEMVVPLREEAIAVLSTQGWQKTALTSLHLLDS

FLKETQRLKPINVTSLHRYATRDTTLSGGIHIPQGTNLGIHSYNLFNPDIYTSPETFDGYRYLRLRQSDPKWEHKGHLVS

TSVEHTGFAHGKNACPGRFFAANEVKIAMVHVLLKYDVKVERKEDAMWITYGPNCYVNPKARMWVRRRREEVDLEALARE

 

>CYP598B2 Mgr073 Mycosphaerella graminicola

55% to Mgr069, 41% to CYP598A1   Magnaporthe grisea, 41% to 68D2

LQPKSRYPIINAPKTSWTSIPAKIEYLKNTKHLIIEGYRRSSGPFKLQTEHGISVVLPPSALKMVDRSEIGLSGPEFLRRRFLSTYTTFQAMGNPPHDLLPEAVLRGLTRSLPKFTKPLSLTMNDCLASSWGSASDWQERALMDDTLLWVARLSTRVFLGERFVQNAEWIRIAQEYTIDFMTATAICGFVPALIRPLFVLGLPWCRKLRRDHAICERMFGPVFAEREAEIEAAQREGRVPDLPDDAIEWCRNAGKGRKYPQLNSQLTLSIAAIHTTSDLLGQTVLNLCVHPELLEPLRQEAISVLKSYGWQKQALTELRLMDSCLKETQRMKPVQLASSNRLATRNVTLPDGTFIARGEVVTFSAHDHFNPDIYPEPEKFDGYRFLKRRAIPGLEHKSVLVSTSEEHSAFSHGKHACPGRFFAANEVKIALVHLLLKYDMKIEKPEMAEWFTFGANTIANSKAKILVRRREAELDLEALA

 

>CYP598B3 Mycosphaerella fijiensis

53% to CYP598B1 Mycosphaerella graminicola

49% to CYP598B2 Mycosphaerella graminicola

estExt_fgenesh1_pg.C_110219 revised at N-term

MVEESRLWQSLAEGVFHVQREIIYGVLVLILGLFWIVSSLRARGTHFESINE

KKSQWWNLNYKARKDYIQHARRLLSDGFRQ (0)

FKGPFSILTDGGRIIMLPPEMIDAVNEKSELSFQRFTEEHFLAKYDTFRTFRSPP

PGLFEEAVMTGLTRSLPKFTKALSKEMSSCLDDTWNPILSDNEWHEIDIRKDILKWVARLSTRIFLPSHFAEDPEWLRVS

VEFTTDAFMASTICRFMPRIIQWPMERFFPLCRKVRRDYRTAASILQPVLDERHKEISTAQQEGRKPNLPDDAIEWFRNA

SHGTKKYHEVDIQLSLSVAAIHTTSDLLLQALLNLGTHPELLNPLREEAISVLKKHGWQKTALHDLHLLDSFLKETQRLK

PINMVTMHRTATSPVHLSNGLKISQGARTAISSHKMWSASIYADPETFNAYRFLDPTMGHRRHLVATSPEHLGFAHGKHA

CPGRFFAANEVKIAMVHLVLKFDFKLEDAGMARWVEYGTAMIANPKAKMWVKRRRDGGIDLEGLAM*

 

>CYP598C1 Mycosphaerella fijiensis

38% to CYP598B3 Mycosphaerella fijiensis

38% to CYP598B1 Mycosphaerella graminicola

37% to CYP598B2 Mycosphaerella graminicola

e_gw1.29.34.1

MGYLISNFIDGTYWISSTSVLVACLSLAGAWTAARHWTSPWKKPRYPTINREEWDLFRHRATQQYITEARTLIKRGLHKY

RGPFTIITTGEPVLVLPPQYTKAVSEHPALSFGDYAQNDNLAFRTWTFSAAKSISVLPEPVLKGLSRKIPTLINTISSEV

SDCLDEMWETTPDWHEIPIKQNIIPCIARLTSRIFLGPTISSNPEWIQTASDYTVDIFLALERVKRWPRPLYFLAEILEP

ACWKARAQYATAARILQPFLQNREESLAQDQAVPEDSIEWIRNFATRKLCSDVDNQLGLTITSVHTMADLLTQAIINLCV

YPDIVPALRAETVRLVQKNADGMIDKSALNDADLLDSFLKETQRLKPFASAALFRRASCDVRLDDEVFLHKGQNVCVAHR

MWDEERYEGAETFKYDRWVRCGGEAGEGLHKRLVATSEDFTAFGHGRHACPGRFFAAAGIKIALMQILLRYDLKPLEWEE

GDLVEHGFSMTTNPAKKIFVRRRGGVGDV*

 

>CYP598D1 Mycosphaerella fijiensis

49% to CYP598B3   Mycosphaerella fijiensis

fgenesh1_pg.C_scaffold_10000124

MDTPTELNSSGEPLQLNAPNAAIHHILAQLTEAKAPSLGLYAAV VITVLLGAQVLQYIIARLRRPKLPTINKFPWDPFGDKAKTQYLSHAKKLIQDGFKK FGERPFYLR

MEVDKTVLLPPQYIEAVNKEDGLSFIAFTTTHFLSHFETFKGLDGTTSMPGHFEEAIMKGLTRSLPRFTIPISREMTGAL

NDTWGNNKEWHEVWLRGDVLKWVARLSARIFVGDPLASDETWLRISREYTSNLFGAVDKVKSYPRPLWWIVERFHPLARQ

TREDRRQAKQLMEPYIAARNEEIARAAKEGRKPDVPDDSIEWFRTSAKGRPYKDVDIQLSLTNAAIHTTSDLLGQSILNL

CKYPDVIPHLREEVIQVLQKYGWQKVALTEVRLLDSFLKETQRFKPVGMVFMARKATRNVSLPGGLNLPKGTDTAVSSHQ

MWSPDIWGDDHDQFNAWRFVERRKQTPFQNSSLLVSTCPEFTAFGHGKHACPGRFFAANEVKIAMMHLIVKYDFKFEDPK

DADWLEFGVTMLANPAAKIWVRAREPEIDLDAVGVMGAEVEVEA*

 

>CYP599A1 Magnaporthe grisea MG01950.4  34% to CYP54A1 38% to MG08459.4

AACU01000379 cont2.368

MSEFPFSSPSTGVFALSKPSHLFAAIALAAALLIVRRLLKPDPYANYPQW

GEELGGFAKRRTYYLHNAAEVYYDAYRKFKSRICKLTTLDGDILLLPIKY

ADELLKLPGDIVDSYELLSSRIESKILKIKPENEYLVHVLRSDLNRQLPR

LNDALADAARTSVLETLGDSEQWTEFSVYRRVMEIVAVVSGSIFIGPELC

RNKEWLEIGIRFTIDLFHAATALKVYPRFLRPVAKYWCPEVFAVHRHTSK

AAELLMPVIENRRGLRDKDPSIVSNDLIHWLVNKAPKWGKDTTEFLVHSE

LQLTMASLNNTTAAITHFLYDIAAKCPEVVPELRAEIQTALDKHGGVWSA

QALFSIKLLDSVMKESQRFNPNLMITPRKILKPIEFRDGTRIPAGVNVCV

AGYAALQDGDFYKDPATFDPYRFLKLRTGETEDPLQYASTEQYQYVSVTK

ENMIFGYGLHTCPGRFFASSAIKMICGQILLQYDVKLPDGVTERYRNLIT

GHFINPDTTKSLMIRKRVM*

 

>CYP599B1 Magnaporthe grisea MG08459.4  poor match 38% to MG01950.4

AACU01001260 cont2.1594

MEDVQELLRSKLQFGLLIGALVVGGLTLWPWIADDRALRKFPLVDQEFGS

RSKRRNRFVQDSIGIYEKGYKMYRDVIFRITESAGEVIVLPIKYLEELRK

IPDDIIRATEATEDVIQTKYLHTTPEDPLLNHILRADLTHKLSQLNERLC

EEAARTVPEFLGKNREWTAIKVNATLLQIVAIVSGNAFVGPDYNRDEGFL

RTTINFSVDLFGTAYQLRAWPWFLRFIGQYRLPGVKAVKEHKRRAKEFLE

PIIKERRARLAAQEGYQEADDMLQWMIAKADKFNVVSDADLADLQLTLSM

TAIHTTTMMTTWVLYDLMYFCPEVIEDLRTEIKTVLARHDGALNTQAMSE

MMLLDSVMRETLRYNPVNVRAFQRYVRKTVKLSDGTVLPKGSHIGTPMHN

VHFDPDLHPDPLVYNPYRFYDIRTGKVPDPLGYSDPKQHQFVSLNKEINT

FGYGRHACPGRFFASNEMKLLLARILLDYDIKMPEGQTTRYQNVVSSGAV

MPDPTRDLMFKYIGK*

 

>CYP601A1 Magnaporthe grisea MG00238.4  31% to 54A1 poor match AACU01001391 cont2.57

MFVVRPGVDLLVQRPWIAVFLAAALLTAVCISRRRKPDDLEHIPCSGQGT

PRKRLQEYRLHAKRLYGDGYKKFQSLYRAANRIGYYVCVPYHLLKSYASA

SEEVLSPHAPFEEFVEASITQINVTSQALIHS VKRDLTPSIATGKATPEL

SRVARDAVLSEIPQSSDWTEVDVHHQLVRIVTIVSGWMFVGPDFCRTDEY

MDLAAHYAADAFGGPLVLQMFPKALRSIVAPLVPPVRRVWKAHRRIAALL

GPAIAQRQRNTGPGSQPAPDDMLQWLIDNRHQFPDEIKGDGDIARLQLAL

NFVVASLYDLAAEPEIMQDVREEIHRVLQEHDGKITSKMLFDLKLLDAVC

KESQRLNPADLRMCLVPYPPPPNHVTQSDHRCSVSARRRVMQPFTFSDGT

AVPVGTMLAVPVYHIGRDPALFPQDPEKFDPYRFTRLRSKDAEGGGNHLQ

FASVTHGTMAFGWGKHACPGRFFAAAEIKLILLHMLLNFEVSVVPGPDGV

KRYPNVEMGNMNMPDFKRRVLFKSIRG*

 

>CYP602A1 Magnaporthe grisea MG09198.4  poor match AACU01000639.1 cont2.1756

MQTVNHSSAIFGRYNFFSRMDATMEGPAGDQIGTQFHHLS

49369 YYIALACILLGAYFFKLQKNAKRSQVDAPYYKASIWKWMFDAETQIIESYNK (0)

FYGKVYQIKATEGMQLVLPPAFVAELKSLPEETLSATAAMCE (0)

AMLSEYTHFHFANNGDLLAAL

49939 VKTKLAQNATNSTIVPRLKEEIDVLV

AEEFPECRDWTTFKIQPFTLRAVTRMAGRIFVGPEINRREDWVETNINFV

VHVFMTVAKLQFMPQFLRPIGQYLVPDVRRTAADLEKAGNLLKPIIDQRM

RDKENFPGYEAPADMISWLLETLSDKDRADIAVHAQLQLMLAMASGHGTN

NIIVDCIFDLADDPDLQDELREEVEAAYATGAPGSDWTKEKLLMLKKMDS

LMKEVQRFRGNLI 50655

50738 GFHRKVLKPISLSDGTYLPPGTKLLVPQAGISRDGAF

YQYPDHFDPMRFYKLRQQQQQSSRDSSGSGDEDSARHHQFTAIVDTNVNF

GGGKHACPGRFFACTEIKLLLAYFLRNYEVRLKPGEGRPKPFTMMMSKVP

NPDGEVQFRRRECAEY* 51199

 

>CYP602B1 Fusarium graminearum FG01868.1 AACM01000100 FGcontig1.100_scaffold1

MQNTSSLPASPVGVNVQYPQLVLEGKRTGYYITFTLFILTLWLILPKKQR

SQLEVPFYKASKKNWIFDAETLIKDSYTKF RDRVYQIKATEGVQVLVPAS

LVGELKGLPEDVLSATEAVSDALQSKYTKFSPGHNGETLALLIRTKLTQN

LTRVVPRLREELEYILATEFPACEDWTPVKWQPFSLRAITRLSGRAFVGP

SLNRDEKWMDTTINFAVHVFTACVKLQFIPEWARPVGQYFVSELRQIRKD

IKIAKELLEPILKERLQNMELSNGEDAPDDMIQWLLEALPKDEKSDLTTQ

AELQLIIAAASIHTTNNLLCECLCDLAAYPEVQEELRQEAYQILEAENGW

ERKESLAKLKKLDSFMREVQRLSGNITNQYAASFIRKVMKPISLSDGTQL

PAGTKVLAPQAGIALDERYFPDPERFDAFRFYKLRQESSE

ANHRWQFTSL

DDTYTNFGAGKHACPGRFFANNEMKLVLAHLLINYDIRLKPGQDRPKPMT

VVMAKAPSPDTELEFRRRSLVA*

 

>CYP602B2 Nectria haematococca

e_gw1.13.286.1

Necha1/scaffold_13:161330-162748

78% to 602B1 small gap after GNIT (5aa) check Fusarium graminaearum  seq for error.

MENVSSLLASPISVDVQLPQVVTGGKRASYYITIAIVLLAVWLIRSRRKPLELEFPFYKA

PKTKWIFDAETLIKDSYTK

FRDRVYQIKATEGVQVLVPARLVGELKVLPEDVLSATEAVSD (0)

ALQTKYTKFTPGHNGDMLALLVRTKLTQ

NLTRVMPQLKQELEYILATEFPACEDWTPVKWQPFGLRAVARMSGRAFVGPSISRDEKWMNTTINFAIHV

FTACVKLQLFPEWARPIGQHFVSELGQIRNDIKTAKEMLLPILEERLRDMDMPGSEDAPDDMIQWLIEGL

PEEEKADVTVQAELQLILAAASIHSTNNLLCECLCDLAAYPEVQDELREEAYRILEVEKGWEKKENMAKL

KKMDSFMREVQRLRGNI (1)

TSFIRKVMKPISLSDGTQLPPGTKVLAPQAGISVDERYFPNPERFDALRFYNL

RQESDEASNRWQFTSLNDTNINFGAGKHACPGRFFAGNEIKLVLAHLLINYDIRLKKGEDRPMPMAMVMT

KAPSPDAELEFRRRSLAV*

 

>CYP602B3 Fusarium oxysporum

84% to 602B1, one frameshift

FOXG_04264 revised

MENVSSLAASPIGVDYQLPQ &

FVSEGHCTGYYITITIFLIAIWFFLPKKQTSHLEIPFYKASKTKWIFDAETLIKDSYTK (0)

FRDRVYQIKATEGVQVIVPARLVGELKGLPEDILSATEAVSD (0)

ALQSKYTKFSPGHNGELLALLVRTK

LTQNLTRVVPLLKKELEHLLATEFPVCEDWTPVKWQPFSLRAVARLSGRAFVGPSINRDEKWMDTSINFAVHVFTACVKL

QFIPEWARPVGQHLVSELRQIRKDIKIAKEMLKPILEERLRDMEFSNGGNAPDDMIQWLIEALPEEEKADLTTQAELQLI

IAAASIHTTNNLLCECLCDLAAYPEVQEELREEAYRVLEVDNGWENKESMAKLKKLDSFMREVQRLSGNI (1)

TNHYIASFIRKVMKP

ISLSDGTELPVGTKVLAPQAGIALDERYFPDPERFDAFRFYKLRQESAEASNRWQFTSLNDTYINFGAGKHACPGRFFAS

NEIKLVLAHLLINYDIRLKAGEDRPKAMSVVMAKAPSPDTELEFRRRSWAA*

 

>CYP602B3 Fusarium verticillioides

97% to CYP602B3 Fusarium oxysporum

FVEG_07386

MENVSSLAASPIGVDYQLPQFVSEGHCTGYYITITIFLIAVWFFFPKKQT

SHLEIPFYKASKTKWIFDAETLIKDSYTKFRDRVYQIKATEGVQVIVPAR

LVGELKGLPEDILSATEAVSDALQSKYTKFSPGHNGELLALLVRTKLTQN

LTRVVPLLKKELEHLLATEFPTCEDWTPVKWQPFSLRAVARLSGRAFVGP

SINRDEKWMDTSINFAVHVFTACVKLQFIPEWARPVGQHLVSELRQIRKD

IKIAKEMLKPILEERLHDMEFSNGGDAPDDMIQWLIEALPEEEKADLTTQ

AELQLIIAAASIHTTNNLLCECLCDLAAYPEVQEELREEAYCILEVDNGW

ENKESMAKLKKLDSFMREVQRLSGNI (1)

TNHDIASFIRKVMKPISLSDGTKLPVGTK

VLAPQAGIALDERYFPDPERFDAFRFYKLRQESAEASNRWQFTSLNDTYI

NFGAGKHACPGRFFASNEIKLVLAHILINYDIRLKAGEDRPKAMAVVMAK

APSPDTELEFRRRSWAA

 

>CYP603A1 Magnaporthe grisea MG08385.4  poor match about 40% to MG08379.4

AACU01000341 cont2.1578

MKLRVSQSPTPQMVITMLHGTFKVDADMCEIPKLFCTKGTSKYDNSSSTY

SLLASKKRNSNVVGQYIKNGIPFRMRNPADPSQPQVILPFKYLSEMKNAA

ESSWSFMHFSNQSFLLEYINAPLGSSIAHQVVRGELNKNLDWTALQPYML

FANTIARTTSLVLAGPELSANPEWTTIMVTFTMTLMQTSQEVRAKYSPWL

RWLVPWIHPGAKNLYKIRKRCAQLLAPSYQNRRAGMVGDEKPFMDAIQWL

MNKRTYKSKDLMKLSDDQLFLSVASIHSTSASTLSTLYDLLDRPECMDGI

LHEIRTIRAESKSSDWTKHDLDRLVKLDSFMKESQRYHPVGQVTVQRSNP

RAYEFSDGLKIPANTQTCFLSYELNHDPDVYPDPETFDADRFLRMREKVD

PQKYHFAYVSEDSINFGAGAHSCPGRHFAANEIKLMLCELLLGYEMKWPD

GQSRPPTMFHDFSSNPNPGFDICIRERRL*

 

>CYP603B1 Magnaporthe grisea MG08379.4  poor match about 40% to MG08385.4

AACU01000340 cont2.1577

MLDMSQPGLLTTKALFVLAVAGITLAVCRLLLRFNVLPFGNDVPPATADQ

LRHIPLLQFDGPNGVERYVSETRSLLRKGYEKYLRRGVPFQMRNPVEELG

AQVVLPPKYLDEVKRAPTDLFSFEAYSEKAFLLNYSRAPRQTEAAAHIVR

VDLTRNLGALVTDLWNESALYLDKTYNSEWQTKQAYEVVCGFVARVTSVA

MVGAPLCRNPVWNRIVVETTMASFGAAQAIKDKYSARWRWLAPWSESIQK

DLRRIRKESIELLKPLYEDRKAAVSRSDDVQGSSEMFRDTLYWLITSNQK

DRSLSGITESQLFLSLAAIHTTSATLNSFVYDWIAHPEYHGEILAEVKET

LAQVQLNGGKWTLQHVAMLRKLDSFMKESARINPIGFVSIQRYTLKPYTF

KDGFQLPAGVSFVFHSDGVHHDADNYPDPEKFDAYRHLHLRETVDPNRFH

FASVSDSALGFGAGNHACPGRFLSAIIMKFFLIQFMTAYEMKYEHGGIER

LPNHDNSNTTAPNRTVNLLACDSSKEYAKALENNEVPALILLG*

 

>CYP603C1 Aspergillus clavatus

51% to CYP603B1

ACLA_078710

MMITLFTLAVVSIGFFLWWLLTVQPAVTKRALKHIPELRFEDNDTPERYTQDSRSLLFRGYDKYLRHGIPFQMRHPIEEL

GPQVLLPMKYLDEVKYASTSLFSFPLFSEKVFLLNYSNAPRQTDAAAHVVRVDLTKNLGALANGMYQEAIEGLNQSLSAS

EEWNTLPAYDFLSSLTAQVTALALVGPELCRNREWIDISLQTTFAIFNAAFTIRSQYSPRWRWLARWQSDAPKRMRAMRA

RAVELLAPVYQDRLAAIKEHDFGAFADCLHWSLRGNGGETSLTRIAEQQLFLTVASMHTTSSTLTAVLFDLLIRPEYCAE

ITKEMQEALTECEGKWTLQEVAKMRKLDSFMKESQRVHPIGFITAQRMTVRPHTFKDGLHLPAGVIFQFPADAVHHDPAI

YPRPDQFAGYRFLHLRETVDANRYHFASVSDTMLGFGAGSHACPGRFFTSLAIKLILVVLLTQYEVKLADCDGSRPANGF

NDFNMGPSREATIMIRKRSGL*

 

>CYP604A1 Magnaporthe grisea MG02272.4  poor match AACU01000440 cont2.445

MIVETLHITKALVMDILDKASLPHLIFLLISAYWILCQFAPNRNPTLPGA

PIVGKRLWLEPTFLQRWRFVFHARDILSDGYAKYKDKPFVVRKLNGDVTI

MPMRYLTETRLLPESVLSFKFALSKFFLPEQMNLNWIKTSERDPNMLKMK

LTPEISRFIEMTRQEMEQTLPTTMPKPTSWTVVDVQSAIRTIIARMTTRA

FMGHPACDDPEWVKMSIDFTPQLLSTVFAVSPFPAWTHSILTRLLPSRYR

TLQRMRYCSELVAARIEARRGGKTPPDDCLLDYMLDHGGADDVYETGQRQ

CMLALASNHTTAAAAINFLFDLCAHPEYFSILRDEIASVTAAHGRPGSPG

VDVKTWLHALEKMDSFLTESLRHTPAILTIPQRQAMRDIVLQDGTLIPAG

ARIAFPSYWHTHEAKPTLRDPDVFDGLRYYKLRRGEGGDPMRYRSVQVDA

DHMMAFGYGSQACPGRHFATSQVKLLVARLLEEWDFAWPDGLEKPDIGYF

EENTLLDPGHKLKVRLRGAEPGHETH*

 

>CYP605A1 Magnaporthe grisea MG08387.4  poor match AACU01000342 cont2.1579

MMTPTELITHYRIVQHSFAPLRLSWWETNRVIAVQIWRDSSFFTRILIAF

IGLCLLSIFSRLTRPRSLRRLGIPGAVQPRFSTWSLDFKKVLEDSAKKYP

NSPFCLNAFGTEYAVLPSKCYDEVKRLPEHQASAFAFFREAFHGAWTGAG

VQTPELGRTIAVELTRGIPSLVHWRQMDCAEAFKMCIGEPSEWREIQLFE

AIQRIVISVNSSSFVGRELGTNQNWLRLIYNMPLQLGIPTVILGWTPFLL

QPLLKPFLFAPLRMTQRKIKSMLRPVLENDVQEYEASSDKKNLLSPKEQG

KVQLTGWLLSRYKGKLDFEVLLQDFITVLFESTPSTASTLFHVVCELAAD

PALQDMLRQELEEHTDNGSLPQTHLNELRKMDSVMRESARASPFSYSTNQ

KDPYPPVVLYRKLSIPTKLSMGPELPAGTNICVDAHHINTSPDLWDQPHT

FDGLRHYRARQQPQNENRYKFANLGSDAPSWGDGLQACPGRMFADNTIKI

ILTHLLLNYDVKLRPGESKPEKGAMPNGSIVPDVWAKVLFRSRASSAVNE

GKK*

 

>CYP605B1 Magnaporthe grisea MG08378.4  poor match AACU01000340 cont2.1577

MLSLLETKIIEPFMVVRQTLAPLRLSRWQIFKHMTRILIFSSNTRTIIFC

VLMSLVGYIVSRIIWGRQEKYPDHGLPIVKTNDYHFDNIVAEGKRLYPNQ

AFMGINKRYKFVIYPSSSWEELKRIPEQTASIMDFQHVCNSGEWSLVGGE

THELVKTITAELTRSLPARVPNRQQDAKMTFDTIIGHCPEEKGFNLLMTS

LEIIAKINACTFVGRELGANKSWVTAVVYSPLWVYFAVTLCNATPDILRP

LLRPLFFLPALRNYWNMQKLLKPKLDREMETFRQTDDKRKLLVPKSDQDL

PFTHFLLSRYTEAAATIKQLVIDYIQVSYTSTPTTASALFHALWELAQHP

EAAEVMRRELATVMIDGNLPKTHLQELKRMDSFLRESFRLHPITRFTLQR

YVKEPFQLSDGSRIPPGVMAVVDAQEINRSPEIWENPDEFDMDRFYRLRE

ISGNDNRYHFVTTSSNSPGWGDGTQACPGRFFATSTLKIVMAHIVMNYDV

SLRKVAPLKSQPLVNGSYSPDDSVEIFFKSRNVE*

 

>CYP605B2 Aspergillus clavatus

53% to CYP605B1

ACLA_078670 revised

MWSLQSNIIEPYQVLRQSLEPLKLSRWQMTKLIARSMVNEISP

MVNEISPRTLVLLAVTCSLLVLYFSTRERQPVMPRELPVVKAKSYHFEDIIVEGRKKYPDRPYLAVNNRHSFVVYPPSCF

DEIKRLPEHSASAKDFFHTMNAGDWTYVGHETTPLLKTIIADLTRVIPARVNKRQQDTRMAFESIVGYAPEWKEIGLLMT

TFEIVAKINACAFVGRELGTNNKWVKAVMQSPLVIHVAVLIMNACPALLRPLLAPLAFLPTKMNQWDMRRLLTPMLQEDM

AIFKETKDRSELLRPKQNGKIPLTAMLLSRYKQGEATIRQLIVDYILISFDSTPSTASALYHVICELAAHPEAADVLRQE

LDEVMVDGKLPQTHLQELKRMDSFLRESFRLHPVSLFSLQRVLAKPVKLSVGPTIPAGAIIAVDAAAINRSPSLWKDPDE

FDMNRFHDLRQVPGNENKFHLLNTGSDSPGWGDGTQACPGRFFANSTLKIAFAYILQNYDVKRKEGSSPPKMTPLANGTW

APDDKAAALFKSRN*

 

>CYP606A1 Magnaporthe grisea MG04349.4  poor match 58% to MG03104.4 AACU01001098 cont2.822

MLENVLTVEIAVLSAFATCYFLYQTLFKPGNLPSFAIAGAKEGDWFPYFQ

AKIRNTADGQNAVFSAYKQHREEPCFLPFFGTPRGTVVLPLNWIKSILSQ

PDHVLNLYDVGVEMLQLEYAFLDKRFNNPPLHINLISKDLTKNYGDLIPD

MMEDIKVAFTEQWGRSDEWTTVTVYDTVQKILGLPLRPLVAPLITLPHRL

SLRRFRKIVVPEIQKRLADSNRGDTKAQQNDFLQWAIQQAKEFEDPYFSR

PEVLASRALFANYISIHTSTFSFTDLVLDLAMLGPQVQEELRQEIVSVLA

SCDGKWSKKALAKMEKLDSLLRESARVNTFMSGSSNRFVTAPEGLALPNG

QTLPKDTPIIFFAAPVMLDETYYPDPYEFQPFRFAPKATDDGDGGDDETG

AQDGKRARTAFACTSPEYLAFGHGKHACPGRFFAAAELKLMAAHILLEYE

LEKIERPASKWYSIVRLPSMTYELKIRRRL*

 

>CYP606A2P Magnaporthe grisea MG03104.4 (version4) poor match 58% to MG04349.4

AACU01001602 cont2.617

MEKLDSTLRESARLNSFVTFGSNRLVTAPQGLTLPNGQTLPRGVGIMLQA

YPVLHDEGIYPDAATFKPFRFAEARRDESADYVQRARNAFATTSPDYLAF

GHGRHACPGRFFAATELKLLLAHMLLGYDIEPRQRPRNKWYGIGRVPDME

ATVRIRRRKRE*

 

>CYP606A3P Aspergillus terreus

53% to CYP606A2P, 50% to CYP606B1

possible first exon followed by a frameshift (&) and deletion

of most of the P450 sequence

ATEG_00556.1

MIDSLVLDRPAVLFAGLAFVGIIFLRILFSSLLPNIPIVG &

RESQRLNSFVVTATNRMVVNPNG

VTTPSGVRIPKGTMVYAPSYPVMHDPDIYPDPESFKPFRLADKRTALGEEGQSYVQRARQAWTTTSPKYPAFGHGCHACL

GRFFASTLLKLMLAYILMNYDFQFLERRPENMWIGSNRTPPMKATIMIKRRVESEK*

 

>CYP606B1 Fusarium graminearum FG11282.1 AACM01000464 FGcontig1.464_scaffold9

MIQAATDLVSRPAIAVPLIIFLSYITYQLFIKPSNLPDLPIIGARKSDWF

PLFQARIRNSLNVKAVLNSSYAQYKNQATILPLLDGGNVILLPRSDTKFA

SEQPTNQLSMHESVQHDLQTDWTTMTPSLTHDPIHLDLVLTHLTKEVGNL

IPDLAEEIEHCVRQQWGTSSEWTE

 

VCIFEKAQLITSGVTNRAFVGVPLCRNDEMLKLGIAFAQ

 

DIPLSSMLLKPFPNFLKPLVAPIIALPNRIHNNKFER

ILKPEIQARLAKYDAQATESEKPPKSERNDYLQWLIEQAKDIGHPKNWKV

NALSERVLMMNFASIHTTTFAVTHALLDIAASSPDLIAELRDEIKSVLEQ

HNGKWNKRAVAKLEKLDSAIRESQRKNSIVSVAVSRTIVAEKGVTFPSGT

HVPKGHRIAVPGYSVFQDPEVYPEPKKYKALRFYNARQNEKDEYVKSARN

AWPTTSADFLAWGLGRNACPGRFFASSEIKLMIAYMLLNYDIEHLDERPH

NTWIMQNRIPPMKATLRIRRVSQP*

 

>CYP606B2 Fusarium oxysporum

76% to CYP606B1

FOXG_13357

MANPVLELLSRPAIAAPLILIVSYVIYQLFLKHSNLPDLPIIGAREGDWFPILQAKIRNSLDVKAALNSAYIQYRNQAAI

FPLIDGGNIIYLPRSDTKFASEQPTNMLSMHESAQQDLQTDYTTMDPSLTHDPIHLELVTTTLTKEVGNLIPDLAEEIEH

CVRKQLGSSTDWSEICIMESAQKMLSGITNRAFLGLPLCRNEEMLKLGIAFAQDIPLSSMMMKLFPSFVKPLVAPLITRS

NRIHTRDFEKILEPEIKARLEEYDSQDEHTKSDRNDYLQWLIEQAKEIGNPKNWQVSALAQRVLLLNFASIHTTTFAVTH

ALLDIASSSPDLISELRDEVESVLKLHDGKWNKRAVAQLEKLDSAIRESQRKNSIVSIGVSRTVVGEKGVTFPSGTHVPK

GLRIAVPGYSVLQDSEIYPEPKTYKPLRFYDARQNEADEYVKSARNALPTATQDFLAWGLGRNACPGRFFASNEIKMLLA

YILLNYDIEHMAERPRNTWIAQNRIPPTKATLRIRRISRS*

 

>CYP606B2P Fusarium verticillioides

90% to CYP606B2 Fusarium oxysporum = ortholog, frameshift and 5 aa deletion

FVEG_12187

MANPVLEFLSRPAIAAPLILFVSYIIYQLFLKPSNLPDLPVIGARKGDWFPILQAKIRNS

LDVKAALNSAYIQYRNQAAIFPLIDGGNIIYLPRSDTKFASEQPTNMLS &

QQDLQTDYTTMDPSLTHDPIHLELVTTTLTKEVGNLIPDLAEEIEHCVSKQLGKSTD

WSEICVMESAQKMLSGITNRAFLGLPLCRNEEM

LKLGVAFAQNIPLSSMLMKLFPSFIKPLVAPLVTRSNRIHTREFEKILEPEIKARLAEYDSQDEHTESDRNDYLQWLIEQ

AKEIGNPKNWQVSALAQRVLLLNFASIHTTTFAVTHALLDIAASSPDFILELRDEVDSVLRQHDGKWNKRAVAQLEKLDS

AIRESQRKNSIVSIGVSRTVIAEKGVTFPSGTHVPKGLRIAIPGYSVLQDSKIYPKPKTY

KPLRFYNTRQNEADKYIKSARNALPTATQDFLAWGLGRNACPGRFFASNKIKMLIACILL

KYDIEHLTKRPRNTWIAQNRIPPMKATLRIRKRIRS*

 

>CYP606C1 Aspergillus clavatus

43% to CYP606B1

ACLA_061240

MAIITKIVAGLICVYIVRKLLFPSVAHLPKGLPIIGAKDSDWFPFLQAAWRNALNLQGNLLEAYAKHKDQAAILPIAGPG

GQTFVLLPASETKLVTDQPSTVLSLREVIVQGLLYEYTISDNFIVSNPTHTKLITTTLTNQVPNLLPGLANETIRGFEQQ

WGTNTESFRDVTIWDSMGAIVSRATNYAFVGLPYCRDSALLDAGIGFARALPFSQLLLSFTWKPLRPLASFLFTLPCRYY

ENRFTQILIPEIEHRLHHSFNPPSSPCSGETSGEAEKKNDFLEWCIEQAKESGDPRMWQPRTLAGRILLMNLVSIHTSSL

TIVNVMLDLASSTPEVLEELRAEVKQVLDTCGGQWTKLALSKMEKLDSVLRESARLNTLVAVGLRRRVVAKEGFTTTSGV

HLPYGNFCAVHNLGVVHDPNVYEQPDTFKPFRFVGLRRDVNSNDPNKDHVQRARLTFPATGCDYLAFGNGRQACPGRFFA

ASELKLMLAYALMYYDLEMMDSRPPDRWIGILRIPSSNAQLRVRRRKGTVEPSMNNDNLV*

 

>CYP607A1 Magnaporthe grisea MG07247.4  poor match AACU01000236 cont2.1347

MGWAAHLVVLALMGATLLLARLVHATTTRIYRHKHICDKLDCVGGVSATS

STPTWIAALFRSISGLQDAAQEGYDRFVKSTTRGPRPFVLPTMWTGGPIV

VLPPSMLPLVNRSPSHETGVPGLLEQFQFRYLHADPDVWANTAIHFDVVR

RDMAQKNMAPLAATIAGEWAAAFRACWDEDPAAAGKTVGGVSAWDSGVRI

MARVAMRVLVGLTGCRDESFFELSQLYARAFIVDGVAINCLPPVLRPLLA

PVLALRVRYYQRRVVGALVPLVEQRLARAAVEEADKTNHEPGDVIRWLIA

RSAAEGSPEQMQPVKIARRVVALMSMFGFAIGWVFAHALLDIYGGPSRDD

VVAGLEDECDRVRRRHGGSIDTKEAVEALFRVDSAVRESMRLSDVSVHIL

PLDVVAGEGIDLGGGVRITPGCGLRAVFPAQMVHTDPDNYADPKTYDAFR

FSRPFEADVAQQDGGENRDERGKKRELMTTTTATFLPFGYGRRSCPGRWL

VAHMVKQALAHVVLNYHVEVTQRPGPRRALLNFMLPAEAARIAVNRKKNV

QN*

 

>CYP608A1 Magnaporthe grisea MG10356.4  poor match AACU01001708 cont2.1991

MFTPKPQRTLGTSLDGPHPPVVNIPAEVRRSYKVNDAYSKALDQHGPVIM

VPRHGRNEYVIDHRYASVLLTDTKRFNFDKAVFDLLHFGFIAVADNGVFV

NDIDQAVEGNVQPRMNAIIAKIFPVFRQYFDRMEDEVPKPAETKEYTTIS

DMFSRMQLAVAHAMVVMVVGEEHAGPELAGHFAAVAVAMARMTGMDESTD

EWTWFPKLWVLFNGLSAVFLTIVPRFFFTVAPLLWKTRGEHLANGLAARH

GIHVPVFDLLLVKHYHEKQGLWALLGFFRCLIIVIGLIFASIHQTVVGGM

WQLIKLTEKQDEYLAAIRAEFEAVVPPGELMTVPKLAQLRLLDSFIREVF

RTKGDCWGPVRETTQPVRVGPYVIPKGAMCIVLIGRAHQHPDNYGPDGKK

FDGTQWVEKGKPASQTSHDYLPFGHGRWACPGRHLAIHEIKIMLYFLFTK

FDIKLRANTYRVKNTINTTTVAPEATFLVRRRAEYNGTV*

 

>CYP608A2 Uncinocarpus reesii

68% to CYP608A1

UREG_04981.1

MAASDVRLDIQPPLDGPSPPIIHITREVRRSDKVNDAYSQALDEHGPVIIVPRHGRDEYVIDHRYAHE ILTDTKNFTFEK

        TAQRPLSG-TPPIVSLTVCVLLRPLPVLALERQIQQHGPVMGLRRDGRVEYIVSDELTFQ

AAFDLLHLGFIALFDNGMFVRDIDSLVEKNVQPRMNAIIDRIFPVFQSYFDRMADELPNPANDKTPVECYNIFSCLQKAI

GHAMVTMILGPSHSSSVTAGHFAAVAVAMANMTGMHENTHEWAWFPWLWVLLNGFKAVFFTIIPRFFFCIVPSLWKMRKQ

HLATGLAARHGEYVPLFDILLVKHYHEKTGFRALGGFAWCVILCVGLIFASIHQTVVAGSWILIKLAQKQDEYLPAIREE

WDTVAPANEPLSVKKLSQLTLLDSFIREVFRTKGDTWGPLRQTTRPVRIGPYVLPKNAMCIVLISRAHQHPDNYGSEGKV

FDGFQWQRKGRPAVQGGADFLTFGLGRWACPGRQLAIHEIKIMLYMLFSKFDIRVKDGSFRVLDTINTTSVPPEATLLLR

RRQ

 

>CYP608A3 Aspergillus terreus NIH2624 XM_001218190

75% to CYP608A2 Uncinocarpus reesii

ATEG_09569.1

MELQARHTYAALLHFPSLARRVQNPSAKDLDIDLQHYPQVIHFP

NTTGAFCIDRSVIDEDAEATTSPGPLNLTHPEAILSDRTASCVFLYIASRTFASPTPT

PAYPAMFTPLPQLTSETSPGASSPPVVHINREIRRSIKVNEAYSKALDKHGPVVIVPR

HGRNEYVIDHRYAHEVLTDTKNFTFEKAVFEMLHLGFLALCDNGAFVHDVDSLVEKNV

QPRMNAIIEQIFPIFTHYFDRMADGLPNPAVDKTAVEFPDMFHRIQLAVAHAMVVMIL

GQPHASSTVAGHFAAVAVAMANMTGMHENTHEWTRLPWLWVLVNGYSAVFLTIIPRFF

FRIVPTLWKARNEHLKNGLAAKHGEFVPLFDILLTKHYHGTTGLFALAGFFRCVIVSI

GVIFASIHQTVVAGLWILIKLTQKQDEYLPALREEWERVNPAGECLNVKKLTQLTLLD

SFIREVLRTKGDTWGPVRQTTQPVRVGPYVLPKDALCMVLISRVHQHPDNYGTEGTAF

NGLQWAEKGRPAVQGAPDFLTFGLGRWACPGRQLAIHEIKVILYMIFSKFDIKLKGDR

YRVTNTINTTSVAPEATFMLKRRDV

 

>CYP609A1 Magnaporthe grisea MG07210.4  poor match AACU01000230 cont2.1339

MTGEADNPNILHATVSSLSTFQEWRVTTIVVLLLSLFALWLRSMASRPPL

PPSAPELLKTWEWPIFGSWRFFLDRADFFTEAAAASKTGNFSFYIGKLHI

VGVSREESRRILFDSKGLDLAAGVAAFVQSSPSIEAVDGKPLGDDPGFNG

AFNRTMAHLLKGQNFGRNLKFMVSDVHEGLESAIEAAGGSGEGIMDTFED

VNLILYRMTMRTFGATEIASDPALLRKTLGLFEVTERSSSPTRLVIPWLP

TLNHLRRMGSGAMLYWHFSKIVNERKKTGKKVDDAFQYLIDKDEDMTGIL

RFTLSALFAGQINTGINAAWVLVYLALTPSWYSRVQQEVDAVVAKYRTSP

NQAPVDVIRSLNLEQWENELASVRLCLRETIRLHTTGSVFRRNISGQDVS

LGSSGEIVPKGSFAIYSVDDMHMNKEYYPEPERWDPARYEGGNDPPYMGW

GLGRHPCPGMRFAKLEVVIIVTLFVTMFDFEVVDDKGRKTTEVPERARNS

FTAVKPAVPVRLALKTRQ*

 

>CYP610A1 Magnaporthe grisea MG01865.4  29% to 533A1 poor match AACU01000128 cont2.354

MSLNSSSILESASLGSGLPLNKGTLILLLAGVAALLVGLDKLLTPSVSSS

EPWLLKPSIPLIGHIINLLRYENDFHHRLYKQTGRPALTLQMLWGKLYVI

LDHRLGSAAMRASEASFDEFGKDFGEQILALQPDTMRRVNEDPAWFPMFN

RVSAESLAPLRLRDVTSDAADALARVVCETGDGSPLTVPNLYYWARELIT

SATLEAMMGPHGIVTSKDRSLNEYLWAFEGGVASFVTHPLPRFTARKAWN

GRAKVFEAMARYFGGEFDRTESRGMVKARSAISRVTFEAAELPRIETGAI

FPMVTNSGPMGFWFLYFIYSNRDVLARVRAELQAATVLTPNDDDGTQTAS

FNLARVEYSCPFTSACFKEAVRLANNGVQNRRMVGDATLRDGEGRSYLFK

KGTDLQISTGIGHRSREIWGDDALEFRPDRFLRPLTREQKAAYQVWGGGA

HLCPGRHFVLQEVVSMAAVLVVGFDLELTGPLRWEDVKMGQGTMGNNSCK

PVNDAKGLQAKVSRRAGWEKTTWAFEA*

 

>CYP611A1 Magnaporthe grisea MG10355.4  poor match AACU01001708 cont2.1991

MSVPLHERGLLGALAVAKLSDATTRVKDLNGFTLAIAGSVLLIFILDRLL

VPSHDPREPPLASTNIPLIGHIIGFARNSFTYYSMLNEKLCKPIFTILMP

GRRIYVITKPELVVQVDKKHKYFSFGPVVRDFSRDTFGLGKEATEILAQN

MYGEHGSWGLCEEMVAGMREALKPGDDLDRMNRIMAEEVSRSLDETKPDP

GSEFRVIKLAEWVSHVVTLATTKSVYGTHNPFVRKDVRDAFWIFEDHVIS

LLVHPFPQYACKKAIKARQYVTDALAHYYINGHQKEGSALARARFDYSVR

NNVPVEDIARFELGGTIAILVNTLPSCYWMLLLIHLYPELHRELQKEVDA

ALLIDEEKSHITIDITSVKNNCPLLLSTFKESLRYRGMGTALRIVTKDIE

LGGYLLKKDSMVQIPLQVIHSDTGNWGNDPLSFDPYRFLK APGKKLPDDS

GYRSFGGGKHLCPGRFFATNEILAVISLFISRYVMRPKGGGDWILPTTKN

SNAARQILQPDFDMEVEVRNRPGFEKYTWKVELHKSDKVISVLTGDTRGA*

 

>CYP611A2 Uncinocarpus reesii

57% to CYP611A1   Magnaporthe grisea, missing N- and C-terms

UREG_04980.1

MENLNGFTLIVIGATALLVTLHYLLNPKRDPREPPLVPPGIPIFGHLLGF

MRNRFDYYNKLNDRVRQPIFTICMLGRKIYVVTKAELISRADRKHKSISYAPIVSEFCSITCGTSNEATKILNHNLLGEH

GNWGLCEEMVSGIRAALKPGKDLDNISLAMAQEVSCLLDAIKPDPGQGYSTIQLSAWVKQIVTVATTNSVYGPHNPYKSQ

EIKDAFWEFEGGLMLMLAAPFPSYTARKHILARDKVTKALEKYFAERHFDHGSKVAKARFDTSVRNKVALPDIGRFEIGG

TVAILTNTYPAVYWTLLLVYVAPGLLADLRAEIDAALIIDPLKNNITIDITVMKSRCPLLLSTLKESLRYRGMGVAVREV

VEDTELGGYLLKKGAMLQVPIQVVHSDQACWGEDAKSFNPRRFLKDDT

DDTGM KEVPNDIGYRSFSGGKHLCPGRFFATNEILAIVALFVSRYEMRPANGSEWVLPTTFNSNA AAQVASPDFELEMEVRNRVGFEKYSWNVDLKPSKTVVAVLARDRVE*

 

>CYP612A1 Magnaporthe grisea MG10862.4  P450 part poor match AACU01000368 cont2.2095

MTDGPRKGMRKQTSPCRHFFTPKDKAHSGGKSSTGLCRDG

NNGKSKPRIPYIGHVLGAATSHTAYAQRLSAAHPTKPVATLHLGATKCYA

VFHPALQHALMRSRAVKYDEYPPGAVPAGFGVDPRVMAVLSRPDLAARYM

HEAAGMLGGEPLRRMERRAFAHLQSRLNALPRTAATADSLYVWLRDAMSM

ATAEALFGEHNPYRTRAAARDPLSLFRDQWTFERSLGRLLLARGSAAARV

LAPAGCRARDRVHAALAGYYAAGEEAQGAAAVVQGRAALLREAGLSAAEF

APHEITAMHGATSNTIPTLFWTVANVYLCPELVAELRAELEAAVELAPLP

GGAADAKQLVRIRPAELESRCPLLADCVREALRLSSQWIGWRTTHADVEI

SAEQSPDGRPYLLKRGVQVIMPAGITHRAPSVWATATADPESSKDGGGGS

VEEFDPRRFAAQSKHKIKSTKGGLGYVPFGGGPYLCPGRNFANHEILGFV

AALVLGFDIVGLKRDKVKMRPARIYEVSAKPAKGGDGGRVQIQRRNNWEH

VQFEFVFAN*

 

>CYP613A1 Magnaporthe grisea MG07743.4  poor match

AACU01000945 cont2.1439

MSALIMLFLSYGLYLLAATVCVLSLVALAELVIPGYSPKVLRVALSKVLN

IILEHRFPIQTPDGLHSIPSCPYQWPNGQGDDGKFLSGIENSARWRAKHG

SVYRIWSGMHPEVVLTEPGQLSAVFKDSDKHTKAPANNSGAYMDRLLGKC

VGLISGQEWRRVRGVVEGPFGFHAASSPSSSKGIERIVARHFEELARTGR

LRNGLIHPAEDLKMLPFWVVCEALYGRLPDRLVRELNSMAPLREGLFRHV

MQGGLARFGWAAWLPTAANAEMDEFQRRWTAFNEAAYEFAKAQQQQPSNN

SSPHDKLPPIVDMVDKMRAGHITQEHLLQTLDESLYANLDVTTGSLSWNL

VFLAANRDVQVRLRAELQAASDQSPQSKEAYLRSDATLLHACVLESSRLK

PLAAFSVPQAAPSARTVEGYVVPAQTSFVVDSYAINIHSDTWAPDNAAYR

PERFLGKGGGGSELRYKFWRFGFGPRQCMGKYLADNIIRRTLAHVVLGFE

LGWADEGEDWSRARDMWITHPDFCIRCTKSTQERMTAETGAGPHTEV*

 

>CYP613B1  Aspergillus fumigatus Af293

          GenEMBL XP_750856.1 also AY838877

          GliC in the gliotoxin biosynthetic gene cluster

          39% to 613A1

MAFTLTILVPCMVLALVAARPVLYWVLSVVIDAFLRWKYPLPHHAGSKPMPRARYTWPNGQGTEKFFNGR

SAARQWRQRWGPIYQIWSGWCPEIVLTTPTHAVQFFRNSHRHTKAVNNDSGWLFGEVLGVCVGLLSGTDW

KRVRQQVEDGFSRPTAARYTGDLVFLAREYLQNTLLASSEQSLENKGIIHVEPAKTLQFYPFLSVAQILF

GRLSPMQRTQLTTLAPLREELFKEVIRGGINRLSIAPWFKSRGVRLLNEFQTQWEQFVEDAYHAAVKRNQ

SPRPLVIGLWEAYQAGTISKRECLQTLDESLYANLDVTTHALSWNVLLLAENGEAQTELRQEVLSALQSE

ASESYERYIDRDDTFLAACILESARLRPILPFSNPESAPEDLYVDGYLIPAN (0)

TNVIVDAQAINIDNPYWVNGTQYNPRRFFSLNKSDVRHNMWRFGFGPRQCLGKHIGERMLKAIVAEIIRQ

YVISISADSALKNDLQEDSWVGLPATRIQCVPVGREVEKN

 

>CYP613B1  Neosartorya fischeri NRRL 181

          GenEMBL AAKE02000024.1, NFIA_055360

          95% to 613B1 Aspergillus fumigatus, name changed from CYP613B2

MAYALTILVPCLVLALVAARPVLYWVLSVVVD (0)

AFLRWKYPLPHHAGSKPMPRARYT

WPNGQGTEKFFNGRSAAKQWRQQWGPIYQIWSGWSPEI (2)

VLTTPTHAVQFFRNSHRHTKAVNNDSGWLFGEVLGVCVGL

LSGSDWKRVRQQVEDGFSRPTAARYTADLVTLAREYLQSTLLTSSEQSVEKKGIIHVEPA

KTLQFYPFLSVAQILFGRLSPMQRTQLTTLAPLREELFKEVIRGGINRLSIAPWFGSRGV

RLLNEFQTQWERFVEDAYHTAVKRNQSPRPLVIGLWEAYQAGTISKRE (0)

CLQTLDESLYANLDVTTHA

LSWNVLLLAENGEAQTELRQEVLSALQSEASESYERYIDRDDTFLAACVLESARLRPIL (1)

PFSNPESAPEDLYVDGYLIPAN (0)

160249  TNVIVDAQAINIDNPFWVNGTQYNPRRFFSLNKSD (0)

VRHNMWRF  160428

GFGPRQCLG  160455

KHIGERMLKAIVAEIIRQYVVSISADSALEKNVLQEDSWVGLPATRIQCVPVGRE

VEKN*

 

>CYP613C1  Aspergillus oryzae

          GenEMBL BAE56604.1, AP007154.1

          72% to 613C2, 51% to 613A1, 50% to 613D1, 39% to 613B2

MLWILVAVVVIVGISSPFTRRITIEILSTILD (0)

90386 RYLRSRFPIFSVDGSRTLPTCPYKWPNGQGDVAKFLQGIENRDLWEKEHGQ

IYRIWSGMKSEVVLTQPSHLQAVFRDSNKHSKAENNNSGYL MSELLGQCVGLVSRERWRTLRAVTEIPFQ

HDKMPSYLELIQRHTRHHFDRLLASGDLRQERIHPAQDLKMLPFWVVAEIFYGECDAEMKTELQQLCVLR

EDLFKRMIQGGIVRWQWSKYLPTATNRALAEFQRRWRAFNQRAYDRACQQQRILPIVLMIEAAREGSTSV

EQIYQTIDEALFANLDVTTGGISWNLVFFAAHSDIQERVRQEVLSATDHDAYLLSSSTLLAACISESARL

KPLAAFTVPQSAPTDRIIGGYNIPAGTNLVVDTYALNIRNGFW GADSQCYRPDRFLEHRATELRYQYWRF

GFGPRQCMGRYVADLVIRTLLAHLVAHYELGWVEPDPGKNSTWQRDLESWITIPDLQLRCVQRRND

 

>CYP613C1  Aspergillus flavus

99% to CYP613C1 Aspergillus oryzae

AFL2G_07286

MDCFSLDGQMLWILVAVVVIVGISSPFTRRITIEILSTILDRYLRSRFPIFSVDGSRTLPTCPYKWPNGQGDVAKFLQGI

ENRDLWEKEHGQIYRIWSGMKSEVVLTQPSHLQAVFRDSNKHSKAENNNSGYLMSELLGQCVGLVSRERWRTLRAVTEIP

FQHDKMPSYLELIQRHTRHHFDRLLASGDLRQERIHPAQDLKMLPFWVVAEIFYGECDAEMKTELQQLCVLREDLFKRMI

QGGIVRWQWSKYLPTATNRALAEFQRRWRAFNQRAYDRACQQQRILPIVLMIEAVREGSTSVEQIYQTIDEALFANLDVT

TGGISWNLVFFAAHSDIQERVRQEVLSATDHDAYLLSSSTLLAACISESARLKPLAAFTVPQSAPTDRIIGGYNIPAGTN

LVVDTYALNIRNGFWDADSQCYRPDRFLEHRATELRYQYWRF

GFGPRQCMGRYVADLVIRTLLAHLVAHYELGWVEPDPGKNSTWQRDLESWITIPDLQLRCVQRRND

 

>CYP613C2  Aspergillus clavatus NRRL 1

          GenEMBL AAKD02000041.1

          note: this sequence is upstream of the pseudogene CYP-un3

          72% to 613C1, 49% to 613A1

          ACLA_066860

MPWIILALPVLVLVTLLPRKVTNSIFSAIID (0)

24668 KYLRWRYPIRSIDGTHTLPTCPYQWPNGQGDVGKFLQGIENRDAWEKEHG

KIYRIWSGMKPEV (2)

VLTQPHHVQSVFRDSNKHSKAENNNSGYLMSELLGQCVGLISQEPWRHLRAVAEVPFQHD

KAPAYVQLVLRHIRQHFSSLHVHGDLQQGRIHPAQDLKMLPFWIVAEIFYGEIDIAMREE

LQQLCLIREQLFRRMIQGGLVRWRWSRYVPSATNRELTDFKSRFRRFNQQAYQHACNQNK

WSLPIVSMTEAAQSGKVSLEQIYQTIDEALFANLDVTTGGISWNLVFLAAHPEIQNRVRD

EAQTATACEAYLLSSSTLLAACVLESARLKPLAAFTVAQSAPTDRTVEGYCIPAGTNIVV

DTYALNIHNEFWGTDSQSYRPDRFLKHRATELRYQYWRFGFGPCQCMGRYVADLVIRALL

AHLVENYTMGILDGDEDKWKRDSESWITLPDMQLKCEPRDVPMTKL* 23208

 

>CYP613C3 Aspergillus niger

fgenesh1_pg.C_scaffold_11000488|Aspni1

53% to CYP613C1

MDTTILLAGQAVLIVVILVAVKFRTPITNRINRLISAILRWPDGQGDGAKFLQGQSNSERWERQFGAIYRLWSGMNPEVV

LTRPEHIQAVFKDSHTHLKAKNNNSGYLLGELLGQCVGLVSQDQWQRVRAIMEKPFHRNASTTYLPLVKRRTEQFFQELW

DTRDLSRGLLDPADDLKLLPFLVVAEVVYGRLAPDVEAELRRLAPQRENLMKHVIKGGLTRFAWSRFLPTQANRELAAFQ

QRWLAFNELAHRRAVEQKLNAPIIDFFAARDAGQISTQELLHTLDEMLYANLDVTIGGVSWNVVFLAAYRDIAKRLREEV

EQQRAISVDGEVDAYLLDNSTLLAACVEESARLRPLAAFSVPQAIPTARTVGGYHFPAGTNFVVDSYALNIRNPYWGEER

HLYQPDRFLARSLTQARYHFWRFGFGPRQCMGKFVASVVIRAILVHLVEGYDLRMVKPEAMEEWGRNKEIWINHPDMKVR

S*

 

>CYP613D1  Aspergillus oryzae

          GenEMBL BAE58578.1 AP007155.1

          50% TO 613C1, 47% to 613A1, 48% to 613C2

         MGFSIQHGLTKPEILYPFLFGIFAVASLCIATLLFPASFSAASRVISWVLS (0)

4127460  IYLELKNPIRHTETGRNIPGPSYVWPNGQGDIEK YVQGRSRSEQWQRKYGNVYRIWAGMTPEV  4127272

4127213  VLTRPEQLHAVFKDSDKHTKATNSDSGYF

MSRILGQCLGLMAGPRWKLLKGTAAPPFMHPTAVRSIGRIQEHVRAHFHDLETNGNLREGRIHPVQDLKM

LPFFIVAEANYGSLTPAMKSELDSLAPARENLMKFVLFGGLARFNISRFFPTEANRQLRRFRSQWRAFNR

AAYERAREKHPSAMVVQMYDAVHKGVLTEEQVAQTMDETLYANLDVTTGGLSWNLVFLAANPACQARLHE

EISALTPAEEEGYISRNGTYLAACVLESSRLRPALPFTIPQSAPTERVVDGYRIPAGTNYVVDTWGLNVR

DEFWAPDNSTYRPERFLNSSNTDLRYHFWRFGFGPRQCIGRYTADVVIRAILLHLVKHYELQMLEEGDFT

QDPECWITHPDLQVKPN

 

>CYP613D1  Aspergillus flavus

99% to CYP613D1  Aspergillus oryzae

AFL2G_01551

MGFFIQHGLTKPEILYPFLFGIFAVASLCIATLLFPASFSAASRVISWVLSIYLEVKNPIRHTETGRNIPGPSYVWPNGQ

GDIEKYVQGRSRSEQWQRKYGNVYRIWAGMTPEV

VLTRPEQLHAIFKDSDKHTKATNSDSGYFMSRILGQCLGLMAGPRWKLLKGIAAPPFMHPTAVRSIGRIQEHVRA

HFHDLETNGNLREGRIHPVQDLKMLPFFIVAEANYGSLTPAMKSELDSLAPARENLMKFVLFGGLARFNISRFFPTEANR

QLRRFRSQWRAFNRAAYERAREKHPSAMVVQMYDAVHKGVLTEEQVAQTMDETLYANLDVTTGGLSWNLVFLAANPACQA

RLHEEISALTPAEEEGYISRNGTYLAACVLESSRLRPALPFTIPQSAPTERVVDGYRIPAGTNYVVDTWGLNVRDEFWAP

DNSTYRPERFLNSSNTDLRYHFWRFGFGPRQCIGRYTADVVIRAILLHLVKHYELQMLEEGDFTQDPECWITHPDLQVKCVRRT*

 

>CYP613E1 Mgr057 Mycosphaerella graminicola

48% to 613C2

MQGVLRRKHRVASLDGTREIPTCTYQWPDGQGDRAKFLEGGTNSVRWEEAHGPIYRLWSGMRPEIVLTQPHQVQRVFKDS

ANHIKAPANNSGCYMDKLLGSCLGLISGKSWRALRDVVEPPFGHKAMGSLTREIERQVGEHIRKLSTTGRPAHGRLHPTE

DLKMLPFWILCRAIYGPLPLHLVEQIEAMIPLREAAFRRVISGGIHRFCFFRFVSTHARDQLVRLQKDWRAFNQSALEYA

RIHAEKAPIIAMWDAVAEGKVSEVQVLQTLDEMLFANLDVTIGGLSWNFVFLAASPADQSRLRAEILSVNKEGCLEQYVQ

SSHSHLGRCVLESSRLRPLAAFSVPQSSRSTVVLDGFVIPEGTDFIVDTHALNIRGKLWSPDNLEFRPERFSQLKPTDLR

YAFWRFGFGPRQCLGRHIADLIIRHTVVQIVQRFELAILDDKAWERDKAMWISCPDFELSC

 

>CYP613F1 Neosartorya fischeri

42% to CYP613C2

NFIA_074290

Note: this seq does not have an ortholog in A. fumigatus

MIKYMPGGMPLLLQLAVLSIVAFVGYAFRPNSWSNRVVSYVINKILLWWYPIYSIDGRSSIPTCPYRFPDGGGKIKFLEG

ETISRKWAKQYGPIYRIWVGLTPEIIVTRPEDIKTAFKDSGNHRKAPNLNGGWVMGELVGDGVGLISQADWKRVHAVVSP

PFAQRPITYVPLVQSRIDRHFAELSQRHSQDRRTMRLRPAEDLKLLPFWVISDLLYGELPQPMVEELLRITELRTDVFQY

AFKGGLSLFSISRWFSPALRNKLHFFHTRWAEFNRQAYHRAKASDAAAACAIVPLSTAVEEGRISRTELLHTLDEALFAN

IDVTIGSFSWIPPFLAAHADVQRELRDEIKQARSQATATPTQDAWVKYLASNTTLLASCINESARLKPVTNYTYAQSLPT

GREVGGYLIPVGTYVVVDTNALNLRDPGWGSDRGEFRPRRFLEKPRGEFRYRLWRFGFGPRQCIAQALADTVLKVLVAYM

VENYQVKVPEKPTGGESEERKRGDAWFKVAEQQILLEPL*

 

>CYP613G1 Aspergillus terreus

50% to CYP613C2

ATEG_03474.1 gene mosdel too long at N-term revised

MYPLESVRHLVVEQSQLCYILFGAVLAYAGWRWVLRLDILNEAITD

LMSNLLNYYLTRRYPIHHSETGQQIPGRSYRWPNGQGDQGKFLDGIENRAQWAKEHGRIYRIWAGTTPEVVLQTPEHIKL

VFRDSDRHSKAVNNDSGYLMGQVLGQCLGLISGGDWRRVREVCEAAFTHSKAINYLDLIHSRVGDYLQKCTALQRGTLNP

ADDFKFLPFLVVGDILYGHMTQEMEDELRQMAPLRERLFQFVIRGGLARYTWSQYLPTEANHALREFRSRWVAFNQAAYC

RARERGATATPVYQLFTAVSDGMITAANAYQTLDEMLFANLDVTLGGLSWNPVFLAANPAVQEELYENITAAQEAEGPAM

PMYLQDSSTYLAACILESSRLKPLAAFSVPQAAPTARVLDGYVIAGGTNYIVDAYALNVEHEFWGPDRHEYRPGRFLGVK

GADLRYHMWRFGFGPRQCLGKYVADLILRVFLVHLVRNWRLGFEDGQIADSREWKRDLDAWITHPTIELTCTPRGARA

 

>CYP614A1 Fusarium graminearum FG02113.1 AACM01000111 FGcontig1.111_scaffold1

MITPLHAIGFILLGYSGLCTVQLLWNYRKAKAIGLPVLITPIDPSNVPYL

LCSSWLEPLLRKILPFGLGNFVEYNSRDWNYSQIHGLQERIGDTFIIVSP

KQIRVFTGNAKASDDLCRRRKDFVKAVALYKPLEIFGRNVVTTEGDDWVR

HRRITTPPFNERNSALVWDESKRQATDMLNMWASNPKGVVNPQSDTMVLA

LHVLTAAGFGRSYKFGSGLESELENHSLSYRDALSLILGNLFTAVFTATL

NLPTWMLPSKFKKVQDAIINFRQYMAEMVAEEREAMNAGAEEQDNLMSIL

VRASENENKQGKGARHLTDTEIYGNLFSYNLAGHETTSNTLAYATVLLAA

NPEWQKWAAEEVDQVTAGVDLKDLDYETYYPQLKRVLAIMHETLRLFGAA

RAVPKTTLVDQTLKVNGTSYTIPKNTFIGVNLAGLHVSSASWGDNALEWL

PSRWITRDETEGEKMTVMPTGAFLPWAAGPRVCPGKKFSQVEFVAVLASL

LKEYTVEPDADGEGDLKTAASMALMEEAKQSSFNFLLKVKHPEKIKLRQG

PVIMSLHVHNWYLLSLSFLFIPVVTYAVSKLAGRLNTLKEVRLLKDLLVF

FLLQNSYIGQLTAEVQMNKHHEHEADGGKTQESISKTKGASWNTTQSMDM

NTIFTLPGP*

 

>CYP614A2 Fusarium oxysporum

91% to CYP614A1

FOXG_04460

MISPLYAIGGLLLAYIGLSTLQLLYNYKKAKSIGLPVLITPIDPSNVPYLLCSSFLEPLLRKILPFGLGNFVEYNSRDWN

YEQIHDLQERIGDTFIIVSPKQIRVFTGNAKASDDLCRRRRDFVKAVALYKPLEIFGRNVVTTEGDDWVRHRRITTPPFN

ERNSALVWDESKRQATDMLKMWASNPKGVVNPQSDTMVLALHVLTAAGFGRSYTFGSGLESALENHSLTYRDSLSLILGN

LFTAVFTATLNLPTWMLPSKFKQVQDAVVNFRQYMAEMVAEEREAMDAGAEEQDNLMSILVRASENQNKEGKGTRHLTDS

EIYGNLFSYNLAGHETTSNTLAYATILLAANPEWQKWAAEEVDQITAGVDLKDLDYETYYPQLKRVLAIMHETLRLFGAA

RAVPKTTLVDQTLKVNGTSYTIPKNTFVGVNLAGLHTSSASWGDNALQWLPSRWITTDETGEKLAPTPTGAFLPWAAGPR

VCPGKKFSQVEFVAVMACLLKEYTVEPDAEGDLKEAASRALMEEAKQSSFNFLLKVKHPEKIKLRCVRRV*

 

>CYP614A2 Fusarium verticillioides

94% to CYP614A2 Fusarium oxysporum

FVEG_07580

MISPVYAFGGLLLAYVGLSTLQLLYNYKKARAIGLPVLITPIDPSNVPYLL

CSSFLEPLLRKILPFGLGNFVEYNSRDWNYEQIHDLQERIGDTFIIVSP

KQIRVFTGNAKASDDLCRRRRDFVKAVALYKPLEIFGRNVVTTEGDDWV

RHRRITTPPFNERNSALVWDESKRQATDMLKMWASNPKGVVNPQSDTMVLA

LHVLTAAGFGRSYTFGSGLESTLENHSLTYRDSLSLILGNLFTAVFTATLN

LPTWMLPPKFKQVQDAVVNFRQYMAKMVAEEREAMDTGAEEQDNLMSIL

VRASENQNKEGKGTRHLTDSEIYGNLFSYNLAGHETTSNTLAYATILLAA

NPEWQKWAAEEVDQVTAEIDLKDLDYETYYPRLKRVLAIMHETLRLFGAA

RAVPKTTLVDQTLKVNGTSYTIPKNTFVGVNLAGLHTSTASWGNNALQWL

PSRWITADEKLATMPAGSFLPWAAGPRVCPGKKFSQVEFVAVMACLLKDY

TVEPGEGDQAAARALMEEAKQSSFNFLLKVKHPEKIRLRCVRRV*

 

>CYP615A1 Fusarium graminearum FG08183.1 AACM01000329 FGcontig1.329_scaffold5

MAYPIILIGVIGLPIIILLRWLLTYTRTVLKVRRNGHPAIHSGVMIFESV

VRSWYPRIPFLVPMEKFTLKDPFKKFADARSDMIVITEASSPNGIAYLFG

SPKIFREIGRNGDIFLKPLEKIRYRMLNTFGLQLASTQNGTQHERHKRVV

KAVFNNELMENGWQNMRNMWRSLLREEGVYPTAANSDAAPIVRDMKSTML

KVTLGAIGASWFDIDIPWNPAKETQRQNDELMPFAETLKVVWDSPFVQTI

LPLWFMEWSPSLHLRRAAWAQRSLVTHIKNAQAETRRRIEDSKDKAQVQG

RMRKYRNLIDALVDSQNDVEMAEKAEKGYLAPNVGLSDKEVQGNIFSFMV

TGHETSSHTLTWVLSLLAKNTDWQERLYAEVSKVNTLPLNEAESANGAKP

LKCLGYEEMANLPLILAATVETLRMRDLAMQMTRVASRNTTLSYTTWDGD

ATNPSEAKVQQHTITIPAGTRVHLDTAAFGVNPFKWEDPETYNPERHLRE

TEDMNGNKKVTISYEDFIGYSSGSRQCIGKRFAEVTMVCFLAHMILNYRW

EVVPEAGETQEQAKVRASTGSEQFMLTPPAYDLRFIRR*

 

>CYP615A2 Fusarium oxysporum

88% to CYP615A1

FOXG_12223

MASPLLLIGAIGLPIIILLQWLVTYARTVLKVRRNGHPAIHSGIMIFESVIRSWYPRVPVLVPMEKFTLKDPFKKFANAR

SDMIVITEASSPNGVAYLFGSPKIFREIGRNGDIFLKPLEKIRYRMLNTFGLQLASTQNGAQHERHKRVVKAVFNNELME

NGWHNMCNMWRALLREEGVYPAAANSETAPIVRDMKSTMLKVTLGAIGASWFDIDIPWDPAEESQRNTKSDLMPFAETLK

VVWDSPFVQTMLPLWFLEWSPSLYLRRAGWAQRSLVAHIKNAQAETRQRIEDMKDNTEVQGRPRKYRNLIDALVDSQNDV

EKAEKAEKGYVAPNVGLSDKEVQGNIFSFMVTGHETSSHTLTWVLSLLARNTDWQEKLYAEVSKVDTLPLDEDESVGDAK

PLKYFGYEEMANFPLVLAATIESLRMRDLAMQMTRVASRDTTLSYTTWDGDAANPSEAKVHQHTVAIPKGTRVHLDTAAF

GVNPFKWEDPQTYNPTRHLRETEDVNGNKKVTVSYEDFIGYSSGSRQCIGKRFAEVTMVCFLAHMIMNFRWEVVPEPGET

QEQAKVRASTGSEQFMLTPPAYDLRFIRR*

 

>CYP616A1 Fusarium graminearum FG09671.1 AACM01000401 FGcontig1.401_scaffold6

MLVPFLSAVATLMPSLLMGIFILCLLGFSAITFNIFVYPFYLSPLRHLPG

PTDNAFFFGQAAKFLQVPWFPELFCQWSRDHPGAPFIRYLNFANSETLFV

NTIGAYKQVLQTKSAFFVKPAFARQFAHEIIGDGLPFVEGNLHKLRRAAI

SQPFSAPRLREFYPTVQGKAEQLVRVLSQRHDKNGNVEIETNVWKTVLDV

IGLETFGLDLNHLESNESPLFETFTTMMQPSALGHIVNYLNSLVPIRQFI

PIAECVEFSKSCTMVREFILCLINIRRNLGEKGFINNQDALQCLLEHNDA

DWNDESLVEYVLNLLVLGHDTTACSITWAIHELSRRPDCQQRLRDEIKIL

DDTCPMISFNDIDKLPYLHNFVREVLRLYCAVAMAPRQATEDVEIEGVII

PKGTVVQLSPAVMNTHPSVWGSDAQVFNPDRWNGLKGDAASAYAFETFHN

GPRMCIGKQLSVMEMKVMLVEIVHKFEIHKPFGDEFKEVAVAGPTFTLRP

KEKLVVRLVELLYDARYTIQHTPMTYPFPF*

 

>CYP616A2 Nectria haematococca

e_gw1.5.226.1

Necha1/scaffold_5:198240-199706

72% to 616A1, no introns

This gene model seems correct

MLGFAAFNIFVYPFYVSPLRRLPGPTDNTFFLGQTTKFLQVAWFPELFCEWSRAYPEAPFIRYLNLANSE

TLFANTIEAYKEILQTKSGMFVKPDFARKFVHEIVGDGLPFIEGKLHRQRRAAISRPLSTPRLKAFFPIV

RNKAEQLAQVLSEQRNSVGNVQVETNIWKAVLDVIGLETFGLDLNHLEGDQSPLFKAFTNMMQPSLIGHI

VNYLNSLAPIRQFLPIEECRELVQSCTRAREFIIGMIAIRRRTLEKGHVEETQEPDALQCLLDYSDPSWD

DNSIVEYILNLLVLGHDTTACSITWALHELSRRPECQQRLRSEIISFDKTCPSPDFGDIDKLPYLHNFVR

EVLRLYCAVAMAPRQATQDVKIAGVMLPKGTVVQLSPAVMNTNPSIWGPTAQTFDPDRWDNLEETAASAY

AFETFHNGPRMCIGKQLSMLEMKVILAEMVRRFRIEKPLGFEERPVEVAAPTFTLRPKEKLVLRLVEL*

 

>CYP616A3 Fusarium oxysporum

74% to CYP616A1

FOXG_08398

MASVLPLMLSLSMGLVVLGMLVMGMIAFNVFVYPFYVSPLRYLPGPKDNAFFLGQTAKFLRVPWFPELFCKWSREFPDAP

IIRYLNFANGETLFVNSIEAYKQVLQTKSSYFVKPAFAKQFAHELIGDGLPFVEGKLHKVRRAAISQPFSAARLRAFSPV

VQQKAEQLIDVLTQQRNEHGNVEIESNIWKAALDVIGLETLGLDLNHLGSDESPLFDTFTTMMQPSTFGHVVNYLNSLIP

IRQYVPLKECIEFSQSCTRVREFIVGLMTIRRNQCEQGLVNEKHRDALQCLLEHSDPDWNDKNVVEY (0)

VLNLLILGHDTTACSITWAVHELSRRPDCQQLLRDEITSMDNTCGMSSFSDIDKLP

YLHSFVREVLRLYLAMAPRQATDDVEIDGIMIPKGTVIQLSPAVMNTHPLVWGPDAQEFNPDRWSDLTGDATSAYAFETF

HNGPRMCIGKQLSLLEMKTMLVEMVRKFKIEKPLGNEEKQVDVAGPAFTLRPKENLVVRLVEM*

 

>CYP616A3 Fusarium verticillioides

90% to CYP616A3 Fusarium oxysporum = ortholog, possible gc boundary at VEY

FVEG_06280

MTSLLSLVPYLSMGLVILGMLVMGIIAFNVFVYPFYVSPLRHLPGPKDNAFFLGQTAKF

LQVPWFPELLCKWSREFPNAPIIRYLNFANGETLFVNSIEA

YKQVLQTKSSYFVKPAFAKQFAHEFIGDGLPFVEGKLHKLRRAAISQPFSAARL

RAFSPVVQQKAEQLIDVLTQHRNENGNVEIESNIWKAVLDVIGLET

FGLDLNHLESDESPLFDTFTTMMQPSTFGHVINYLNSLIPIRQYVPMK

ACIEFSQSCARVREFIVGLMTIRRNQYEQGLVSPEKNRDALQCLLEHSDPDWNDKSVVEY (0)

VLNLLILGHDTTACSITWAVHELSRRPDCQQRLRDE

IAGMDSTCDMPGFSDIDKLPYLHNLVREVLRLYLAMAPRQATDDVE

IDGIMIPKGAVIQLSPAVMNMHPLVWGQDAQEFNPDRWNDLAGDATSAYAFETF

HNGPRMCIGKQLSFMEMKIMLVEMVRKFKIEKPLGSQDKQVEVAGPAFTLRPKENLVVRLVAM*

 

>CYP617A1 Fusarium graminearum FG01048.1 AACM01000052 FGcontig1.52_scaffold1

MGLPWEIITATSAISTLTLAWTHDDWSKSQLLGRFPVFWIAGLAVWIVWA

VWVYPLLVSPLRHLPGPSGNHWLMGQSKKIMAEPSGVPMRQWASEIPNDG

LLCYRGFFNQERVMVSSPKALAEVLVTNSYAFPKPSHFRWSIGRILGVGV

LLAEGDEHKMQRRSLTPAFAFRHIKNLYPVFWRKAREVTRTMMAEFGQQE

ETQVEISGWASRATLDIIGLAGMGRDFGAIQNPNNTLAQTYSKIFKPSRQ

AQILAFVGMIIPMEFITKLPFRRNEDIAKAASDIRAICRDLIQEKKAKMA

NKEQADVDILSVALESGGFTDENLVDQLMTFLAAGHETTASAMTWAIYMM

ARHSDIQTRLREEIREHLPSVDSDVDITSLDIDRMPYLNAVCSEVLRYYA

PVPLTMRDAAYDTTILGQTIRKGTRIVIVPWATHFDHDLWGPDADQFNPD

RWLSAGGENKIGADRKAASGGANSNYAFLTFLHGPRSCIGSSFAKAEFAC

LLAAWIGRFEFSLANPEEMDEKNVEIRGGVTARPAKGMHVKVKVIGGY*

 

>CYP617A2 Nectria haematococca

e_gw1.2.235.1

Necha1/scaffold_2:1188558-1190265

73% to 617A1

This gene model seems correct

MGLPWENITGGAATVTLALSWVQNDWSRLLLLKHFTGLWTVGFVFWVVWAIWIYPLLVSPLRHLPEPSNN

HWLLGQAKRIFAEPSGIPMREW (2)

VSEIPNDGIIRYRGLLNQERILVCSAKALAEVLVTNNYAFEKPSYLQQ

SIGRILGIGVLFAEGDEHRVQRRNLTPAFAFRHIKDMYPIFWRKAKEVTQAMTTEVGGRDEVELEVSSWA

SRATLDIIGVAGMGRDFGAIQDPESVLARTYRKIIKPSRAAQILGLISIFIPLQIVTKLPFRRNQTINAA

ADEIRAVCRDLIQEKKAKLANKEQPDIDILSVAIESGGFTDENLVDQLMTFLAAGHETTASAMTWAVYML

SRYPEVQSRLREEVRERLPSVDSDSDITSLDIDRMPYLTAVCSEVLRYYSPVPLTMRDAAHDTTILGHPV

RKGTRIVIVPWATHFDQHLWGPDADRFDPERWL

PSGPEGGAADRNAASGGATSNYAFLP

FLHGPRSCIGQSFAKAEFAALLAAWVGRFEFALANPEEADESKVEIRGGVTARPSKGMHVKVKVMGGF*

 

>CYP617A3 Fusarium oxysporum

85% to CYP617A1

FOXG_00341 revised

MGLPWERITATAAIGTLSLAWAQNNWARLQLLGHFTELWIASLAIWGVWTVWIYPLFVSP

LRHLPGPSGNHWFMGQAKKIMAEPSGVPMREW (2)

ASTIPNDGLLCYRGFFNQERVMVCSPKALAEVLSTNSYAFPKPSHFRWSIGRILGIGILLAEGEEHK

MQRRSLTPAFAFRHIKNLYPVFWSKAREVTRTMMAEFGGKEEVHVEISSWASRATLDIIGLAGMGRDFGAIQDPQNTLAQ

TYKQIFKPSRQAQVLAFIGMIIPMQFITKLPFRRNEDIAKAATDIRAVCRDLIREKKAKIANKEQADVDILSVALESGGF

TDENLVDQLMTFLAAGHETTASAMTWAIYMLSKHSDIQSRLRDEVREHLPSVDSDVDITSLDIDRMPYLNAVCSEVLRYY

APVPLTMRDAAYDTTILGHSIPRGTRVVIVPWATHFDTELWGQDAGQFKPERWLPSGGESGSADRKAASGGASSNYAFLT

FLHGPRSCIGSSFAKAEFACLLAAWIGRFEFTLANPEEMDEKKVEIRGGITARPAKGMHVNIKTVSGY*

 

>CYP617A3 Fusarium verticillioides

96% to CYP617A3 Fusarium oxysporum = ortholog

FVEG_01179

MGLPWERITATAAIGTLSLAWAQNNWVRLQLLGRFTELWIAGLAICGVWTVWIYPLFVSP

LRHLPGPSGNHWFMGQAQ KIMAEPSGVPMREW (2)

ASKIPNDGLLCYRGFFNQERVMVCSPKALAEVLSTNSYAFPKPSHFRWSIGRILGIGILLAEGEE

HKMQRRSLTPAFAFRHIKNLYPVFWSKAREVTRTMMAEFGGEGEGEVHVEISSWA

SRATLDIIGLAGMGRDFGAIQDPQNTLAQTYKQIFKPSRQAQ VLAFI

GMIIPMQFITKLPFRRNEDIAKAATDIRAVCRDLIREKKAKIANKEQADVD

ILSVALESGGFTDENLVDQLMTFLA AGHETTASAMTWAIYMLSKHSNIQ

SKLRDEVREYLPSADSDVDITSLDIDRMPYLNAVCSEVLRYYAPVPLTMR

DAAYDTTILGHSIPRGTRVVIVPWATHFDTELWGQDAGQFKPERWLPSGG

ESGSADRKAASGGASSNYAFLTFLHGPRSCIGSSFARAEFACLLAAWIGR

FEFTLANPEEMDEKKVEIRGGITARPAKGMHVKIKTVSSY*

 

>CYP617B1 Fusarium graminearum FG02118.1 AACM01000111 FGcontig1.111_scaffold1

MAPIAVFLSYYRPWAAIAIVVAVYYQIIVDQNYSVKNAPFYLGKYLLGFL

SIWAVYTVFLYPALLSPLRHLPQPKPGSFINGHWAESVREPAGLPFRHWM

RTIENNGLIRYKHLFNQERILVTSPEGLKEVLGQNSYDYVKPHLLRAMVG

KILGYGLLLSEGNVHKMQRKNLMPAFSFRHIKELYPVFWSKAQELVHGIE

KEMSEAPGSQIDIADWASRASLDIIGSAGMGHEFKSLSDPSIEDTMKMYG

SMVKQSGGAKLLTVLQLVLPSMITDYLPFQRNMGVLAASKAARDTSQRLI

NAKKVQMAAKEKLSPDIISTALESGHFTDEGLVDTMMTFLAAGHETSAAA

LTWTIFLLAKNHGIQDRLREEIRQNVDGLADDVDSKKLDGLSYLHAVCQE

SLRLYAPIPFTVRDSLRDTTILGTFVPKGTMIILCPWAINRAHESWGADA

DDFYPERWMVPGQANSGGAKNNYANLTFLHGPRSCIGQKFSLAELMALTC

ALVGRYRFDIDKDYEVKDLTDGIVAKPREGLKVSVEEIQGW*

 

>CYP617B2 Fusarium oxysporum

87% to 617B1

FOXG_04464

MARIAVFLSYYRPWALCALAFAIYVQIIRDKNYSIRHAPFYMGKYLLAFLSVWAFYLVFLYHRFFSPLRYLPQPKIAGFI

NGHWAESVSQPAGLPFRHWSRTIENNGLIRYK (2)

HLFNQERLVVTSPEGLKEVLGQNSYDYVKPHLLRAMVGKILGYGLLLS

EGDVHK (0)

MQRKNLMPAFSFRHIKELYPVFWSKAQELVHGIEKELKEESTSEIDIVDWASRASMDIIGSAGMGHEFKSLADP

SIEDTMKMYGSMVKQSRGAKLLTVLQLVLPSIITDYLPFQRNMGVLAASKAARETSQRLINAKKVQMAAKEKLSPDIIST

ALESGHFTDEGLVDTMMTFLAAGHETSAAALTWTIFLLAKNHDIQNRLREEIRQNVDGLTDDVDAKKLDSLSYLHAVCQE

SLRLYAPIPFTVRDALKDTQILGTFIPKGTMVILCPWAINRAHELWGPDADDFNPERWMAPGQANSGGSKNNYAFLTFLH

GPRGCIGQKFSLAELMALTCALVGRYKFDIDKDYEVRDITDGIVAKPSKGLKVTVEEVQGW*

 

>CYP617B2 Fusarium verticillioides

95% to CYP617B2 Fusarium oxysporum = ortholog

FVEG_07585

MPRIAVFLSYYRPWAVCALAFAIYTQILHDKDYSIRHAPFYMGKYLLAFLSVWAFYLVFLYHWFFSPLRHLPQPK (0)

GASFLNGHWAESVSQPAGLPFRHWSRTIENNGLIRYK (2)

HLFNQERLVVTSPEGLKEVLGQNSYDYVKPHLLRAMVGKILGYGLLLSEGDVHK (0)

LQRKNLMPAFSFRHIKELYPVFWSKAQELVHGIGKELKEESTSEIDIVDWASRAS

MDIIGSAGMGHEFKSLADPSIEDTMKMYGSMVKQSRGAKLLTVLQLVLPSI

ITDYLPFQRNMGVLAASKAARETSQRLINAKKVQMAAKEKLSPDIISTALESGHF

TDEGLVDTMMTFLAAGHETSAAALTWTMFLLAKNHDIQDRLQEEI

RQNVDGLTDDVDAKKLDSLSYLHAVCQESLRLYAPIPFTVRDALKDTRILGTFIPK

GTMVILCPWAINRAHELWGPDADEFNPERWMAPGQANSGGSKSN

YAFLTFLHGPRGCIGQKFSLAELMALTCALVGRYRFDIDKDYEVRDIADGIVAKPSKGLKVFVEEVQGW*

 

>CYP617C1 Fusarium graminearum FG10461.1 AACM01000435 FGcontig1.435_scaffold7

MSVIGLWLVTVTATLSLFVWQLIFLLSIPKSIVVCLIAESLFFVAWFFYW

TVIYPRYLTPFRHLPTPASRSILTGNQNGLFTENSWDVARRVSQTVPNSG

LIRYYVALSNERILVTNTRALSDVLTNHSHDFGKSNLAKFALKRLTGNGL

GFLEGNEHKVHRKNLMPAFTRKHVKELTPIFWDKAMEMVKGMEAEVRCGK

DTSTQGTGIVEIHDWATRATLDIIGTAGFGYDFGTLHNPSNEIGQQYKKM

FLEPSTAFNWLELLGNYIDFRFLMTLPVKKNRDLTAGSNFMREIAKKVIR

ERRHELFQRMTSQAGNMKNTKKDIITTALASDCFTDDQLVDHVMAFLVAG

HESTATAFEWAMYELGHRPEMQKRVRDEVRTYLPSPSAGGVKNITFESVP

YLQAICNEVLRLYPFLPFATRVAEKDTWVADQFVPKGTIVAYAAHISNRD

SELWSGPALDAFDPERWMEPGKESSGGANSNYAMLTFSAGPKSCIGEAWT

RAELPCLVGAMVGSFEIELVEGKQADGTVYPTVDFKMGKVLKSRDGVFVR

LRRLEDW*

 

>CYP617D1 Aspergillus nidulans AN1884.1 38% to 547A1 46% to 617A1 547 clan

MAPIPWGMLYVSVVGYSVLTSNLFTFFVLATALTALKIVYASFLYPEYLT

PIKHIPTPPLTAQKRSWVRGNTDTYVVISPFEGMLNWTKSVPNNGLLRYY

IIGNIEQVLVTTPKALSELLVQNAYDYQKPESIRISLARIAGEHGILLVE

GQEHKRHRKNLMPAFSYRHIKDLYPTFWAKSVEMVKCIEKDLQDRRDTGD

ITVTVRPWASRATLDIIGLAGMDRDFGSLADPQNELAAQYHRILEDPPLW

LKLLFAAAFVLGNEELVMALPVKRNRDIAEGAKYVRQVAQQLISEKRERI

KHNPEKAEGGGGGKDILSVALNSGNFTDIELIDQMMTFLAAGHETTSSAL

QWSVYALCKHPDIQTRLRQEIRSNLPSVSSNDPKPITAEAVDSLPYLHAF

CNEVLRFHPSVPITFRTTTRDTTLAGTLLPKGTQLTISPEVINHDPDLWG

PDAHIFNPDRWLGPGRANTGGASSNYALMTFLHGPRSCIGQGFAKAELAC

LVAAMVGRFEMELADPGKKLAVRRTATISPVDGVVARLTPLDGW*

 

>CYP617D2  Aspergillus fumigatus Af293

          GenEMBL XP_749561.1

          61% to 617D1

MTVKIPWPAVYLAIAASVYMRPQYAILDSLTATFVVLSLAVTSFRIIYAFVLYPKFFTPIKHIPTPSNWS

WLTGNTSSYLIESPFEEMIEWAYKVPNNGLIRYYIFGNLERVLLTNPKALSELLVTKVYEFPKPQLVRQS

LARVTGKHGVLLVEGDEHKRQRKNLMPAFSYRHIKNLYPIFWSKSIEMVKLIEKDLHSRADAKDNIVRVS

EWASRATLDIIGVAGMDHDFGSLHDSNNELTKHYRRLLEEPPLAMRIIFVLGVLFGSVGVVESLPLKRNR

EIRESSLYIRDVARQMIRKKEAKMKSQASAETGTDIVSVALESGAFTEEELVDQMMTFLAAGHETTSTAL

QWSVYALCKHPDVQTRLREEVRTHLPPISPEHPEPLSAATLDSLPYLNAFCNEVFRFHPSVPGTVRDAAK

DTTLLGYPIPKGTRILVSPEVINHSKELWGPDADQFNVERWLGSGRANTGGASSNYSFLTFLHGPRSCIG

QGFAKAELACLVAAIVGRFQMELKDADAKLEVRRSATVAPKDGVLARFTPLEGW

 

>CYP617D2 Neosartorya fischeri

95% to CYP617D2  Aspergillus fumigatus = ortholog

NFIA_081070

MTIKIPWPVVYLAIAASVYMRPQYTILDSPTATFVVLSLAVTSFRIIYALVLYPKFFTPIKHIPTPPKWSWLTGNTSSYL

IESPFEEMIEWAYKVPNNGLIRYYIVGNLERVLLTNPKALSELLVTQVYGFPKPQMVRQSLARVTGKHGVLLVEGDEHK (0)

KQRKNLMPAFSYRHIKN

LYPTFWSKSIEMVKLIEKDLHNRADAKDNIVRVSEWASRATLDIIGVAGMDHDFGSLHDSNNELTKHYRRLLAEPPLAMR

IIFVFGVLFGSVGVVQSLPLKRNREIRESSLYIRDVARQMIRKKEAKMKSQASAETGTDIISVALESGAFTEEELVDQMM

TFLAAGHETTSTALQWSVYALCKHPDVQTRLREEVRAHLPPISPEHPEPLSAATLDSLPYLNAFCNEVFRFHPSVPGTVR

DAAKDTTLLGYPIPKGTRILVSPEVINHSKALWGPDADQFNVERWLGPGRANTGGASSNYSFLTFLHGPRSCIGQGFAKA

ELACLVAAIVGRFQMELKDPDAKLEVRLSATVAPKDGVLARFTPLEGW*

 

>CYP617D3  Aspergillus fumigatus Af293

          GenEMBL XP_754149.1

          55% to 617D1

MGPIHNYFGVVCLGIAASVYFRPECALYGSRIATFAVLLTGIAISKLLYQLFIYPQFVTPLKHFPAPPNR

HWLTGNTGSLLVDTPHALMKEWAKTIPNDGILRYYIVGNMERLTVTSPAVLREILVSKAYEFAKPLVIQQ

TLRRVLGNGILIAEGEEHKFQRKNLKPAFAYRHVKDLYSVFWSKGTEMTKLIRNDLQSRKAPDDNTIQVR

TWASRSSLDIIGLAGMGRDFGSLQDPENSLSRSYEMIFATPGLGTKILFILGMLLGNTTWLAKLPTKQNR

LIDTGCRNIRDATRRMIREQKAKMEDPNAAAEVDIISVAMRSGNFDDDNLVDQLMTFLGAGHETTAGALQ

WAIYALCKHPDVQSRLREEVRANLPPIHGENPGPIDAATIDSLPYLNAVCNEVIRFHPSVPNTVRVALND

TTLMGHPIPKGTQVVISPELVNHMPALWGPDAERFNPDRWMGPGKANTGGAASNYAFLSFLHGPRSCIGQ

VFAKAELACLLAAVVGSFAFELKSPDAPLEVREGATIAPKDGVLAKFTPVEGW

 

>CYP617D3 Neosartorya fischeri NRRL 181

NZ_AAKE03000007, NFIA_062490

93% to 617D3 Aspergillus fumigatus = ortholog

54% to 617D1, 46% to 617A1

19kb from PKS 51% to chr 6 Nectria PKS

name changed from CYP617D5, revised seq 3/11/2009

MGPIRNYSGVLCLGIAAGVYFSPECAIYGSRIATIAVLITGITISK

87863 LLYQLFIYPQFVTPLKHFPAPP (0) 87798

87742 KRHWLTGNTGSLLVDTPHALMKEWAKTIPNDGILRYYIVGNMERLTITSPAVLSEILVSKAY 87557

87556 DFAKPLVIQQTLRRVLGNGILIAEGEEHK (0) 87470

87411 FQRKNLKPAFAYRHVKDLYPVFWSKGTEMARLIRKDLQSRKAPDDNTIQVRTWASRSSLDI 87229

87228 IGLAGMGRDFGSLQDPENSLSQSYEMIFATPGLGTKILFILGMLLGNTTWLAKLPTKQNK 87049

87048 LIDTGCRNIRDATRRMIREQKVKMEDPNAEAEVDIISVAMRSGNFDDDNLVDQLMTFLGA 86869

86868 GHETTAGALQWAIYALCKHPDVQARLRDEVRANLPPINVENPGLIDAATIDSLQYLNAVC 86689

86688 NEVIRFHPSVPNTVRVALKDTTLMGHPIPKGTQVVISPELVNHMPALWGLDAEQFNPDRW 86509

86508 MGPGKANTGGAASNYAFLSFLHGPRSCIGQVFAKAELACLLAAVVGSFEFE 86356

86355 LKYPDAPLEVREGATIAPKDGVLAKFTALEGW* 86257

 

>CYP617D4  Aspergillus oryzae

          GenEMBL BAE57429.1

          53% to 617D1, 63% to 617D2, 54% to 617D3

MMGLPWATVYIVIAGYAIAKSTFGNFILLSILVSLLKVFYNWVLYPDFFTPIKKIPSPPGRSWITGNSDT

VFLEAPYEHMRKWLENVPNDGLIRYYVSINLERILPVGPRALKEILVTKSYDFPKPEFIRASLKRLAGEH

GLLLVEGDDHKKSKKNLLPAFAYRHVKEMYPIFWSKSIEMVRVMDEDLRKKADPTDNVLRMGAYASRAAL

DIVGVAGMDHDFQSLRDPNNKLVRTYQNLMSEPPLYMKIIFLLTLLLGDPAVVHDLPLERNRSIERSSET

IRDVARQMIRQKRAKWESGSSTAEDIDIVSVALRSGNFTEEELVDQMMTFLGAGHETTSTA LQWCVYVLC

KHPDVQTRLREEIRANLPPISTENPQPPAATDIDNLPYLNAVCNEVLRYHPSVPATIRCASRDTTIIGEP

IPKGTLFLIAPEIIGKSKELWGPDADKFNPERWLGPGRANNGGADSNYANLTFLHGPRSCIGQGFAKAEL

ACMVAVVVGKYHMELKNPNAPLEIRQQATVCPKDGVLAKFTNIEGW

 

>CYP617D4  Aspergillus flavus

100% to CYP617D4  Aspergillus oryzae

AFL2G_02761 revised

MMGLPWATVYIVIAGYAIAKSTFGNFILLSILVSLLKVFYNWVLYPDFFTPIKKIPSPPGRSWITGNSDTVFLEAPYEHM

RKWLENVPNDGLIRYYVSINLERILPVGPRALKEILVTKSYDFPKPEFIRASLKRLAGEHGLLLVEGDDHKKSKKNLLPA

FAYRHVKEMYPIFWSKSIEMVRVMDEDLRKKADPTDNVLRMGAYASRAALDIVGVAGMDHDFQSLRDPNNKLVRTYQNLM

SEPPLYMKIIFLLTLLLGDPAVVHDLPLERNRSIERSSETIRDVARQMIRQKRAKWESGSSTAEDIDIVSVALRSGNFTE

EELVDQMMTFLGAGHETTSTA

LQWCVYVLC

KHPDVQTRLREEIRANLPPISTENPQPPAATDIDNLPYLNAVCNEVLRYHPSVPATIRCASRDTTIIGEP

IPKGTLFLIAPEIIGKSKELWGPDADKFNPERWLGPGRANNGGADSNYANLTFLHGPRSCIGQGFAKAEL

ACMVAVVVGKYHMELKNPNAPLEIRQQATVCPKDGVLAKFTNIEGW

 

>CYP617D6 Aspergillus niger

e_gw1.4.269.1|Aspni1

61% to CYP617D2

MAGLPWNAIHVGIAVFTYFRSTFRNFLLLSAVTLVLRVFYRLALYPVYFTPLKHIPTPPGRAWFTGNNDSFILVNPYERM

RRWNQDIPNDGLIRFYLAGNLERMIATNPKVLSELLVQKVYDFEKPKIVRQSLGRITGEHGVLLVEGAEHKQQRKLLMPA

FSYRHIKNLYPVFWSKGIEMVKLIEADLQRRANPADNVIRVASWSSRATLDIIGLAGMNRDFDSLQNPTNQLAVAYHKLN

SVPPTPLEKGLFVIGLILNVPHLVHKLPFKRNRYIRESAAYIRNVAQEMIHDARQSIEGEKTHQTTDAVDILSVAIESGG

FTDEELIDQMMTFLAAGHETTSGALQWCVYALSQYPDIQTRLREEIRANLPPISVEAPEPISAATLDSLPYLHAVCQEVF

RFHPSVPNTVRIANRDTTLVGQKVPKGTVIQIVPALTNHDKSLWGPDADQFNPDRWLGPGKANTGGATSNYAFLTFIHGP

RSCIGQGFAKAELACLLAAFVGRFQFELEDPKKELILREAATVTPKDGVRVKVTPLEGW*

 

>CYP617D7 Aspergillus clavatus

77% TO CYP617D5

ACLA_043510

MGLVGNHLGVLYLAVAAAVFFKPDYAVWGSRIATVILLFTAITVSKVVYQLFLYARFFTPLKHFPTPSNRHWLRGNAPSV

LVDTPHAQMKEWAKNVPNDGIIRYYIVGNMERLTVTNPTALSEILVSKAYDFAKPLVIRQALARVLGNGVLIAEGDVHKF

QRKNLKPAFAYRHVKDLYPVFWGKGAEMARRIRKELQDRKSPEDNTIQVRNWASRSSLDIIGLAGMGQDFDSLRDPENTL

SRSYEMIFATPGLGTKAMFMLGILLGDATWLAKLPTKRNKLIDAGCRNIRDATRRMIDDQKRKMQDPTAEARVDIISVAM

RSGTFDDETLIDQLMTFLGAGHETTAAAVQWAIYALCKNPAVQTRLRAEVRANLPPINLDAPTPIDAGTVDSLPYLNAVC

NEVLRFHPSVPNTVRVALKDTTLLGKPVPKGTQVVIAPELINHMPDFWGEDGGQFNPDRFMAPGMANTGGASSNYAFLSF

LHGPRSCIGQGFAKAELACLLAALVGSFEFELRDPAAPLEVREGATIAPRDGVLARFTPLDGW*

 

>CYP617D8 Aspergillus clavatus

78% TO CYP617D2

ACLA_090250 revised

MVTMIPWPAVYLATAAGVYLRPQYTIWGSSIATFILLSIVITTIRVVYSLILYPSFFTP

MKNIPSPPTRHWFTGNTSSYLIRTPFEEMIEWARTVPNTGLIRYYIVGNKERILLTSPKALGELLVQKVYDFPKPEMVRL

SLSRVTGKHGVLLVEGEKHKKQRKNLMPAFSYRHIKDLYPVFWSKSIEMVKLIESDLRRRADTEDNVIRVSEWASRATLD

IIGVAGMDHDFGSLRNSQNELTKHYERLLSEPSVGMRIIFVVGILLANLQVIQSLPLKRNREFRESSQFIRDVARQVIRE

KESKMKDGQSAPGSGIDIVSVALESGAFTEEELVDQMMTFLAAGHETTSTALQWSIYALCKHRDVQTRLREEVRSNLPAI

SAENPEPLSAATLDSLPYLNAFCNEVFRFHPSVPGTMRETARDTSLVGYPIPQGTRILISPEVVNHSKELWGPDADQFNV

ERWLGPGRANTGGASSNYSFLTFLHGSRSCIGQGFAKAELACLVAATVGRFEMELKDPEAKLEVRLSATVAPKDGVVAKF

TPLEGW*

 

>CYP617D9 Aspergillus terreus

64% to CYP617D4

ATEG_05929.1

MAVPFGVIYIALAAYTLTRSTINNFLLVSVITALAKLVYNLVLYPDFFTPIKHIPTPATRSWLKGNTRSIFIESPFAQMR

KWQKEVPNDGLIRYYITGNLERILLTKPKALSELLVYKVYDFPKPEFIRSNLAPITGEHGVLLVEGDEHRKQRKNLMPAF

AYRHIKDLYPIFWAKSTEMVRTIEKDLKNRTDPTDNVVRVGTWASRATLDIIGVAGMDHDFNSLQDPDNNLVQQYHKIMT

EHPKYVKVAFVLAMVIGVPNLVSKLPFKVNIQTKESSEVIRAVARDMIRQKKEKLENKTAKNSVDIISVALESGTFTEDD

LIDQMMTFLGAGHETTSTALQWAAYALCKHPDVQTRLREEVRSNLPSISTDNPEPLSAATLDTLPYLNAFCNEVLRFHPS

VPATIRIAARDTTLVGAHIPKGTLILLAPEVVNHSHELWGPDADQFNPDRWLGPGKANTGGATNNYAFLTFIHGPRSCIG

QGFAKAELACLVAAMVGKFQMELKYPDAPLDVRRGATVCPRDGVLAKFTPLEGW*

 

>CYP617E1  Aspergillus fumigatus Af293

          GenEMBL XP_753139.1 also EAL91101.1

          45% to 617C1, 36% to 617A2

MISFLGLWLCTVTLASGGIFHQALAGGEHTALHFQVLGAHVVAFVAFAVYW

MILYPRYFTPFGNIPTPSRRRILTGNYPVLFPDNAWVPLRELAETTPNDGLLRIYSALSGEALLVTSPPA

IRDMLTVNAFDFTHQDLVKIAIRRFTGSNLERTQLHRKNMMPAFTVPHVRKLTSIFWAKAQEMVRCMSNE

LRADSFARIDFREYMSRATLNNIGLAGMGHDFQTLKQPDTDLRSHHRKLILDPTRVFSWVGLLSRYFDMR

LLMRVPLKKLIEISQSAKYLRELTTAVIQGRREQLVVAENNRGKDIITVALAGGVFDEHHLVDHVMTFLT

AGHESTATAFEWTMYELGRRPEMQSRLRDELRATIGTDLAAVNFGLRVQNLPYLNAFCSEVLRCYPFSPI

IVKVAQKETMLIGQRIPKGTVVLYSAEVSNHDKTLWGPDADKFDPERWMGAKKAKSGGASSNYAMLTFGA

GPRNCIGANFARATLECLVAAVLSTFEIELANPDTAGRLKFGQTKKSAEGIYGRLKFVQPVEV

 

>CYP617E2 Aspergillus niger

estExt_GeneWisePlus.C_100429|Aspni1

80% to CYP617E1

MMITFLEFWLCTLCLTSGGVVHQVFTGGQHIALLFQVLGGHLVTLLGFALYWTVLYPRYFTPLSNIPTPSHRSIFTGNYP

VLFPDNAWVPLRRLAETTPNDGLLRIYSALSGEALLVTSPPAIRDMLTVNAFDFAHQDLVKIAIKRFTGSNLGFLSNDDF

KIHRKNMMPAFTVPHVRELTSIFWTKAKEMVHCMSTELRADPLAPINFREYVSRATLDNIGLAGMGHDFQTLKQPDNDLR

SHYRKLILDPTRVFSWVGLLSRYFDMRLLMRVPLKKLIEVSQSASYLRSVTKKVIRERSEKLFESDDGRTKDIITVALTS

GVFEERHLVDHVMTFLTAGHESTATAFEWTMYELGRRPEMQTRLRDELRESIGTNDLGATDFGSQVQALPYLNAFCSEVL

RCYPFSPIIVKVAQQDTTIMSHPIPKGTVVLYSAEVSNHDKKLWGPDADQFNPERWMGEGTAKSGGSSSNYAMLTFGAGP

RNCIGANFARATLECLVAAVVTTFEIELANPHTAGRLKFGQTKKSAEGVYGKLRFVQPVEA*

 

>CYP617E3 Aspergillus clavatus

77% to CYP617E2

ACLA_098900

MIGFEMSAILAFAVYWMVLYPRYFTPFRNLPTPPQRRILTGNYPALFPDNSWLPLRKLAENTPNEGLLRIYSALSGEVLL

VTSPPAIRDMLTVNAFDFAHQDLVKIAIKRFTGSNLGFLSNEDFKLHRKNMMPAFKVSHIRKLSHIFWQKSQEMVRCMAD

ELRLNPDTSINFREYVSRATLDNIGLAGMGHDFNALKEPENNLRTHYRKLILDPTKVFNWVGLLSRYFDMRILLKVPLRK

LVEISKSANYLRGITGEVIQQRKEKLVADEDLRDKDIITVALASGVFEEDHLIDHVMTFLTAGHESTATAFEWTMYELGR

QPEMQKRLRAELNEAIGSDLAMTDFEKRVQSLPYLNAFCSEVLRCYPFSPIIVKVAKQSTTITGERIPKGTIVLYSAEVT

NHDKRLWGPDADVFNPDRWMGEGNAKSGGAISNYAMLTFGAGPRNCIGLNFARATLECLVAAVVITFDIEPANPDTAGKL

KFGQTKKSAEGVYGRLKLIRP*

 

>CYP617F1  Gibberella moniliformis (also Fusarium verticillioides)

          AF155773.4

          fumonisin biosynthetic gene cluster,

          FUM15

          46% to 617A1, 43% to 617B1, 42% to 617D2, 37% to 617C1, 32% to 617E1

          possible GC boundary after GNRE to remove short intron before heme

          signature (lower case seq)

MRGLNNIAVALALSAIWSFGLDLGRHGTSGSSLLLALFRTLCKA

YPFVVISHFVWSAIIWPTFFSPLRQLPNVPSDGWLSKETLRLVSEPRGVPQSDWINSL

SNRPVDLARYRSFLGFERLLIISPKALAEVLTTKSYDFRKPGLIVSELKQATGMGVLL

AEGSEHKSQRKALQTAFNYRHIKNLYPVFWDVAGEFATVLEKQIPTGTPRTSDTTAVI

DIVDWASRATLDIIGRAGMGQGFDAIQNDDSRLHQAYRMIFEPSRGAIFLALLRLIFP

ERLVNWLPLRRNKRMRHGIQVIRSKCQELIRERKEKIKRQKAGVDNSGNDILTVALLN

GVFTDEQLIDQLMTFLAAGHETTATALTWAIYILCKQPEVQNRLREEIRMHFPNPKGW

PRSERPSSNTLQQAIDFKLPYLNVVCLEVMRYFAPIPLTMREATCDTTILHTFVPAGT

RIILAPRVTNRDSALWGPDANNFNPDRWLSPKNGNRE (0)

ARSNYADLTFLHGPRSCIGQSFARVEFAILLATLIANFEFQIEDESL

LDERNISISRGATSRIVGGLKVRVRPIAVV

 

>CYP617F2 Aspergillus niger

e_gw1.1.329.1|Aspni1

54% to 617F1

MSGIVSGGATLLTAILSYDSPKISLGTIWFQIPLLLLLTWVTWRRLVWPNFFSPLRHLPSPHNRGWGMNQRLRVYTEPRG

MPQCDWVNSIPNNGLIRYRTLLNSDRLLVTSPEALAEVLTTKCYDFKKPKWLANELKKVLGVGLLLAEGDEHRAQRKILA

PAFSFRHIKNLYGVFWDKSCEVVTAMTDQLSCKEGPKTYGPRESSIVNTGVLDIAEWSHRATLDMIGIAGMGRDFGAVHN

PNADLVRAYALVFQPSKQDIMLAVLRLLLPNWMVDWLPLRRNEEIRCAVQRIRGECADLIRQKSKHQRKYHTSQHRDILD

VALSYGGFSDTLLIDQLMTFLAAGHETTATAITWAVYLLCVHPEVQTALRSEIREKLPSLEDSGSNLTSDNIDTMTYLNA

VCDEVLRYAAPVPLTFREAVIDTNILGQPVPKGTKIILVPRATNRDANLWGPDAQQFKPDRWLEGGIRSNYATLTFLHGP

RSCIGRSFAKAEFSILLAALVGRFEFQLEDDQLLDEKRMKVTRTVTARPANGLLVKVTSLAGW*

 

>CYP617G1 Mgr061 Mycosphaerella graminicola

50% to CYP617A1   Fusarium graminearum

MNTLIRLAAAHVTSFYLTTRTSLPVPGLYLTTFLLFLTQFFLTTIYNVVLYPRFLSPLRHIPQPPGSHWLTGHTRKIMREPSGFPAREWVKSVESKGGLIRYSNWGRERVVPCTPAALAEVLVTKNYDFVKPDQLKHGLGRILGRGILLTEGEEHKFQRKNLSPAFAFRHIKEIYPVFWKKSQELAAYGDAKDEEHKHALGTINVGNWSSRATLDIIGLSGMGQDFNSLADPSNKLSQTYANVLRLPPGNILTKILQFAPMFLPHWLLAALPIKRNADLNEATTVIKQTCRELIASKRALLEKSEKSDDTSNRDILSVALQSNAFDDENLVNQLMTFLVAGHETTATSMIWAIYALCKYPDIQTKLREAIRKSIPSLDSDITAQDIDDCHYLQAFCQEVLRLWPPVSLTLRIAGRDTVIQDQVLPKGTTVLLVPVAVNVSEELWGSDAMEFNPSRWLNADGKCNASGSAKSNYSFLTFLHGPRSCIGQKFAVAEFACLLAAWIGKFNTTFEEGCPHAEGEVQIKGGITAKPAGGLWCNVEEVEGW

 

>CYP617G2 Mycosphaerella fijiensis

61% to CYP617G1 Mycosphaerella graminicola

e_gw1.1.212.1

MAPVKQQLSVAIISTILLQRLAAFPLSYPASIAALFFAQWAAYTLCMILVYPRYFSKIRHLPEAPNPHFLLGQTRRIMKE

PSGIPMRDWAQDVPNHGLIKYSVWFQERVLITTPAALSEVLVTKNYDFVKPWHFRHGLARILGIGILLAEGEEHKIQRKN

LSPAFSFRHVKNIYPVFWKKTEHISDTDNMDAEKAAQLHERGVIDVGNWASRATLDIIGLSGMGRDFDSLHDPGNKLNQT

YKSIFNPGRAGRLLQVLGIFIPFWIMRRLPIKRNQELDAATSYIKQTCRDLIAKKREAMSEKGAVGEVDILSVALESGGF

SDEDLVNQMMTFLVAGHETTATAMIWAIYLLCKDKKIQDKLREDIRKQIPNLESEIQASDIDDCHYLQAVCSEVLRLWAP

VSLTMRVADKDTTIQGEFIPKGTTVILCPWAINTSKHLWGEDALEFKPERWLNADGKANQKGGADSNYAFLTFLHGPRSC

IGQRFAVAEFACLLAAWVGKYDTSFEEGSPLAKGELEIKGGITAKPKGGLWCTLKEVPGW*

 

>CYP617H1 Uncinocarpus reesii

48% to CYP617D3

UREG_01117.1

MAHAALWLGGSLCLTVLLVSTSSSFQSYTWTFLFLIPCPPGRSLWNGHHEELESLLPGEMIGRWIQQVPNDGLLRYYEAG

NQETLLPTTPKAVADVLTHKAYDWKKTDITQALLKKIIGEGLLLSEGDVHRVQRRNLNPAFSFRHIKNLYPTFWKKGNEL

LQAIEDAIKPLPNDKKVITVGPWMRRATLDIIGLGGLGHNFDCIQNPDSFLPREFEKLLAPPPAAALSTAFRFLGSLLDP

TFIEALPSKHAANAKEASRRIRDFSRSLLQEKSKGLKDDHCSEAIDVISIALQSGVFADETLVDQVMTFLAAGHETTATS

LQYAIHLLCKHPEMQTRLRDEIHSNIPSPNGTLPTTVSATQIDSLPYLNAVCNETLRYYPPAPFTVRQAARDTTVLNTFI

PKGTRISIGILAMNRHPELWGPDAETFNPDRWMGPGKGNTGGASSNYGHLTFLEGPRSCIGSGFAKGEMACLLAALVGRF

EFELEDPNKELEISTGVAFAPKDGVRAKVRVVEGW

 

>CYP617J1 Fusarium oxysporum

51% to 617A1

FOXG_08717

MIETWQIILAGVTLSGYLLASLHTDNQKSMFLLGLLGTLCLETILWCIYR

IFIHPRFISSLRHIPTPEGNHWLWGHGMKLHREPYCSPVREW

NETVPNYG

FLRYLGFFNAERIVLTSPEALHEVLVTQNYSFPKPASLRETAGRFLGIGLILSEGDAHKLQRRSMNPALAPRNIKALYPL

FWDKTREMIERITADRLETVEVVEWASRITLDLIGVAGLGRDFGAIQDGHNEMVKTYNIVFQPSAQARMLHLIESLVPPW

VLTALPIKLNSDMSQAARSIRETCRDVISSKQKKLKEKKLDDMDIISAAIRTGTFTEDGLIDQAMTLLAA (1)

GHDTTGAAFTWGVYL

LAKHPEVQDRLRHEIRQRLPPLTQAKESPISSVNVDSMPYLQAVCSEILRFYAPVPQTLREAAHDTSILDQFIPKGTRIV

IAPWATNRCSKLWGSDAYLFKPDRWLG

KSAHGGAESKYGFLS

FLQGPRACIGQGFAGAELACLLAGWVGSFEFELRDGVSMDEKNVDVKGGLTARPAGGLHVKVKRLEECRKI*

 

>CYP617J1 Fusarium verticillioides

90% to CYP617J1 Fusarium oxysporum

FVEG_05955

MIETWQIILAGATVSGYLLALLYPDNQNSIFLLGFLATLCTETALWC

FYRILIRPRFISPLRHVPTPEGNHWLWGHG

MKLYREPYCSPVREWNETVTNDGFLRYLGFFNAERIVLTSPDALHE

VLVTQNYSFPKPASLRETAGRFLGIGLILSEGDAHKLQRRSLNPAFAPRNIKAL

YPLFWDKTREMVERITADRLETVEVVEWASRITLDLIGVAGLGRDFGAIQDAKSEMVETYNIVFQPSAQAR MLHLIESLVPAWVLTALPIKFNSDMSQAARLIRETCRDIISSKQKKLKE

KKLDDMDIISAAIWTGTFTEDGLIDQAMTLLAA (1)

GHDTTGAAFTWGIYLLAKHPEVQNRLRHEI

RQRLPPLTQAKEFPISSVNIDSMPYLQAVCSEILRFYAPVPQTLREAAHNT

TILDQSIPKGTRIVIAPWATNRCSKLWGNDAHLFRPDRWLG

ESAHGGAESKYGFLSFLQGPRACIGQGFARAELACLVAGWVGSFEFELR

DRGLMDERNVDVKGGLTARPAGGLHVKVRKLEEW*

 

>CYP617K1 Aspergillus clavatus

50% to 617D2 Aspergillus fumigatus

ACLA_053190

MDTILDFFHHLHAPLMALAIVTVCILRMIHLMILYPTFLTPLRALPTPLNRSWARGNYTNPTASPISQLQHWRAKFPNAG

LIRYYLPGNQERVLVTSVEALNDILVTKASHFTKPMAVRKRLSYITGNGLLLSEGGVHKTQRRALTPAFSFRHVKELYPI

FWRQAMKMADCIESDMTTNIGKGIEVVEVRKWATRATLDIIGLAGLGHDFGSLQNPDNEILRQYQRMRQEPSRVETLLEG

LLSFILPYFDRVVSMLPTRRATIIVEASQCIRALCGKLVQDKKKQNCERLEGQCDIATVALKSGVFTDSELVDQMMTFLA

AGHGTTSHALQWTVYALCKHPGVQERLRAEIRYHLGSPINLNRAVSASDIDGLEYLQAVCSETLRLYPPVPTTTRKSLHE

TMLGGYPIPKGTLFTISPAVINVDPALWGPDSEIFDPERWIGKGRANSGGSRSHHAFLTFLQGPRSCIGATFARGELACL

VAALVGRFQMELEDPKKNEELTKRGVGAAPADGVRVKFKLVDI*

 

>CYP617L1 Aspergillus clavatus

47% to CYP617A1 Fusarium graminearum

ACLA_062310

MTPAQVFTDSTASLPLRILAISSIAWIIWKVLLYPKIFSPLRRFPGPKDSHFLHGQLWRILSEPVGVPMRDWINTIPNDG

IIRITTIFNQERLILTSSEALSDALVTHNYDWDKPEQMKKGLGRIVGQGVLLASGNVHKAQRKHLLPAFSFRHVKTLYPI

FWEKSCEMVEAITKATTNADNTNNKTSISITDWLRRAALDIIGLAGFGQDFDALGKPHSELNNIYEKVFDQPQNENVMKA

IETLENIFPVAWLGSLPLQHNRDVKKASMAIRQAARKVIRSQEQFKTKTASSILSGNSLLSIASRSEYFTEDNLIDQIMT

FLAAGHETVSTATAWALVVLCRQPAIQERLRKEIRSQLPSPSQGTSTFNAQILDALPYLHAVCNEVLRFYPPVPLTRRVC

VRDTTIQGERVPKGTNILIVPAALNVSKKLWGEDALEFNPDRWMGSGKNNGGASSNFANMTFLHGPRSCIGASFAKAEFA

CLLASIVGRFNFKFSDPDYKAEVLSAGITSRLKHELLLTVEVVEGW*

 

>CYP617-un1 pseudogene Nectria haematococca

fgenesh1_pg.scaffold_21000071

Necha1/scaffold_21:299839-300774

possibly derived from CYP617A2

37% to 617A2 mid region aa167-258, 33% to 617C1

36% to AP007157.1e Aspergillus oryzae

LAAEGEEHGRQCRTLVPDFSTRPIKALS

PIFSEKSLLLADPWQDGMKGKKLAAKPFEVLEWLSRATLGIIVKAGLGYDIDSLEHLETPIRIAYQ LMFS

 

>CYP618A1 Fusarium graminearum FG03961.1 AACM01000168 FGcontig1.168_scaffold2

MKAMEASYATILWGVALAVVGFLSHRIIAIALRPHSSRFNGLPRPKGQQP

FAGHALRILRGGGPNDVYLQWTRQWPDAPFIRCLSWLNEEILLVNSLEAC

REVLQTNAYFFAKPGFFHTLVGEFLGLGLLFSVGEQHKRLRRIIAGPLSR

PSIRKLFPTFVTYSQKLNREIGEALERSKSGIVEIEDLIIRVTLDIIGVS

LLGRELRDFRSESSPLSFEQCYNAILAQPLAGQIISFINPFIPLRWLPVS

ANLNFIRAKSALKTMMEGLIEQRTAEVGAAKLMNEDDKLSDDLLTRMIEA

SAEESQKLSKEELIDITMQVIAAGHETTASALVWTAYSLAKDPASQQSLR

AEIHSLGTEMSAKGIDELPFLDNVIREAMRVHSPTLIIPWEAQKDMTIAG

THIPKGTTVQIVPAMIQLNPEIWGSDADVFRPGRWEDMGGNASSPYAMET

FSNGPRMCPGKALALLNMKVLLVGLIRDFEMEIVDDGKEVELRNPSLTLK

AKSLIQFKMRKVS*

 

>CYP618A2 Nectria haematococca

e_gw1.35.9.1

Necha1/scaffold_35:311438-313183

63% to 618A1

This gene model seems correct

MFNTHHLPKALVWTVIIVLVGGILQKIIKAVIQPRFSRLRHLPGPQ (0)

NNQFLVGQALRLLRTPGPNDLFLQ

WMRRWPEAPFIRCLSWLNSEMLLVNNLEACREVLQTNAYSFVKPAFFHTLVGEFLGVGLLFSVGDEHKRLRRIVA (1)

GPLSRPSVRRLMPVFVNESNALNRKIADAIKEHDQGGME (1)

IETFFTRASLNIIGVSLLGKELHSFRSPSSPLTFEQCYSNILTQPIAGQLISFINPFIPLRWLPVKANIDFVRAK

AALKTMMTELIEQRTDEVLAARGGQSDFVLSDDLLTRMIEASFGEDTRLSKQELIDI (0)

TMQVIAAGHETTASALTWITYALATHQDVQNRLR

EELSGIEVTSSAAKAIDDLPYLNNVIREALRVYSPTLMAPWEASENMVIAGVEIPKGTMIQTVPAMVQMN

PSIWGDDAEAFVPERWDTLTGDASSPFSIEVFLNGPRMCPGKALALLEIKVLLMAMIKEFQLEMVDTEME

VRNPSLTLKPKDGLHVRVRRV*

 

>CYP618A3 Fusarium oxysporum

73% to CYP618A1

FOXG_11718

MWPLDLPLVRVVIFVAFAIWLHFIIKTLLRPSFSELRHLPGPK (0)

GHQFLVGHALRILRGSGPNELYLQWMQRWPKAPFIRCLSWLNSEVLLVNNIET

CREVLQTNAYRFAKPAFFHTLVGEFLGVGLLFSVGN

QHKRLRRIIT (1)

GPLSRPSIRKLFPTFMTYSRKLNSEIDAAIQQNSNGVLE

VEDLFTRVTLDIIGVSLVGKELRDFRSKVSPLSFEQCYNGILAQ

PLAGQIISFINPFIPLRWLPVSANLNFIKAKSALKGMMTELIEQRTSEVAKAKQLGTDSGLSDD

LLTRMIEASSVENQKLSKDELIDITMQVIAAGHETTASALLWTTYALSKDQKSQNRLRTEIYSMNATDMTAKSIDELPYL

DNVIREALRVYSPTLIIPWEALEDIDIAGVRIPKGTTVQLVPAMTQLNPEIWGPDVETFNPDRWDRLSGDALSPYAMETF

SNGPRICPGKALALLNMKILLVGLIRDFKIESVGDEEMEFRNPSLTLKSTMTVRFKVQREG

 

>CYP618A3 Fusarium verticillioides

87% to CYP618A3 Fusarium oxysporum = ortholog

FVEG_10106

MWLLSISLVRAVTLVILALCLHFIIKTLLRPSFLQLRHLPGPKNHQFLVG

HALRILRGSGPNDLYLQWMRRWPNAPFIRSLSWLNSEVLLVNNIEACREV

LQTNAYRFAKPAFFHTLVGEFLGVGLLFSVGDQHKRLRRITAGPLSRPSI

RKLFPTFVTYSHKLNSEIDAAIQRSSNGVLELEDLFTRVTLDIIGVSLVG

KELRRFRSKTSPLSFEQCYNGILAQPLAGQIISFINPFIPLRWLPVSANL

NFIKAKSALKSMMTELIEQRTSEVARAKELGTDTTLSDDLLTRMIEASSV

ESQKLSRDELIDITMQVIAAGHETTASALLWTIYALSKDQKSQDRLRAEI

LGLNDDDTTAKAIDVLPYLDNVVKEALRVYSPTLIIPWEALEDIEILGVR

IPKGTTVQLVPAMTQLNPEIWGPDVETFIPDRWDRLSADALSPYAMETFS

NGPRMCPGKALALLNMKVLLVGLIRDFRIESVGDEEVEFRNPSLTLKSKK

PLRFKVRRVSEGS*

 

>CYP619A1 Fusarium graminearum FG09086.1 AACM01000370 FGcontig1.370_scaffold6

MTAYNNVTLSLWALFGALVLYLLTVPGRRGKHLPPGPPTLPIIGNLHQMP

TRKGYLKLAEWAKQYGDMYSFKIGSTTTIVLSNRRLVKEILERRSVISAG

RPAVYGLEKLVFGGHFILLQQPDAPMYRISKKLLHQYFGEPAIDRSHLRI

INAEATQLIRDLMVDPDNFPHHAHRYANSFTMSTTYGIRTPSHDTPHLTA

ITKITAQATSLIAPGRLPPVDVFPFLKLVPERILGNWVTLCTNLGKASDK

LYMYILESVIERRKTKAPRDTFADRCLDNKEYEFSLHELMYLTGAVLDAG

TDTTSSVLITLIQMLCAYPDCLKRAHAEIDMVVGDERTPVWDDFAKLPYI

NQLMKETQRIRPATPISFPHKVLEDIWIDGKLLPKGATIIANVTGLHSDP

NKFKHPGEFHPDHYKGMQKLAQGYANTADENKRDHYAYGFGRRICAGMQI

AERSLFTTFSKVLWAFDISAPKDAQGRPVAMNLDPMTGYTEGTIVMPKPF

KACIKVRSERRRETILREFEEAENNVFAQFDLPKIGEGI*

 

>CYP619B1 Aspergillus nidulans AACD01000084 revised

MNSLPLLLAAASVGFLYVILTKGRREKGLPP (1)

GPPTLPFLGNLHQIPVKGSYLKFTEWASQYGGLYSLKLGTGTAIVITDPRLVKEVID

RKSSKYSNRPESFVAHTITGGSHLLVMQYGPLWRTMRKLVHQHFMETAVE

KSHIHVQNAGAVQMLRDFCVRPDLHMLHPKRYSNSIIMSLVYGVRTPSVH

TAHMTQLYEMMVRILSL

PFLCTQGMPTDRAFVLFQENW

SKVMEPGNTPPV

DIYSFLHYIPQKLFGNWLSRAKEVRDEMCQLYGQYLDLVVSRRKKIGSTG

SFMDTVLDQNEKLGLTRHQLYFLGGVLMEGGSDTSSSIILAFIHAMTKWP

QVLKKAQAEIGNVIGEDRMPAWSDYGSLPYVAATVKEAMRWRPAVPLAFP

HAAAEDDWIDGHFIPKSSTIIVNGWGMHHNEARFGNPSVFDPDHYKGQTA

LAPELANASDYTTRDHYGYGTGRRICPGIHVAERNLFLAISKLIWAFSIE

PGVDESGKVIEPDLDPRTGYSEGFLVCANDFPCRIMPRSEAKRESIMREY

QRAQEEVFSRFESPSS*

 

>CYP619B2 Aspergillus nidulans AACD01000105 revised

MNSLPLLLAAAFVGFLYVFLTKGRREKGLPS (1)

GPPTLPILGNLHQIPVKGSYLK (2)

FTEWASQYGGLYSLKLGTGTAIVIT

DPRLVKEVIDRKSSKYSNRPDSFVAHTITGGSHLLVMQYGPLWRTMRKLV

HQHFMETAVEKSHIHVQNAEAVQMLRDFCVRPDQHMLHPKRYSNSIIMSL

VYGVRTPSVHTAHMTQLYEMMGCRLTMRPSSFQERWSKVMEPGNTPPVDI

YSFLHYIPQKVFGNWLSRAKGVRDEMCQLYGQYLDLVDSRRKKVGSTGSF

MDTVLDQNEKLGLTRHQLYFLGGVLMEGGSDTSSSIILAFIHAMTKWPQV

LKKAQAEIDSVVGEDRTPVWSDYGSLPYVAATVKEAMRWRPAVPLAFPHA

AAEDDWVDGHFIPKGSTVIISGWGMHHNEARFGNPSVFDPDHYKGQTALA

PELANASDYTARDHYGYGTGRRICPGIHVAERNLFLAISKLIWAFSIEPG

VDESGKLIEPDLHPTTGYSEGFLVCANDFPCRIMPRSERRRETIMKEYQR

AQEEVFSRFENISS*

 

>CYP619C1  Aspergillus terreus

          CH476602

          This P450 is two genes away from MSAS polyketide synthase

          6-methyl-salicylic acid synthase used in patulin synthesis

          patulin is a mycotoxin sometimes found in apple juice.

          51% identical to CYP619B2, 39% to CYP619A1

          ATEG_06277.1

MSKWAKQYGGIFSLKRFRNTTIVLTDWKIMKELVDKKSTNFSHR

PPSKVADLITRGNHILMMQYGETWRTMRKLIHQYLMESQCEKEHWKVQEAEAAQMLHD

FLVDPENHMKHPKRYSNSITMSLVFGIRAKSVNDEYMTRLYSLMEKWSLVLETGATPP

VDSWPLLQWIPERFMGYWRRRATEVGDLMTGLYTEVLHVIENRRKAGIYKDSLMDRVL

DKKDKYRFDEHQLAFLGGTLMEGGSDTSSSLILAIVQAMTQYPEVQKKAHAEIDSVIG

TDRSPAWSDFRKLPYINMMIKEAHRWRPVLPLGVVHGLATDDSYNGMHLPKHSTVILN

VWGMHMDPDRFENPDAFIPERYANFPELAPHYAALADGAARDHFGYGAGRRICPGIHL

AERNLFIAVAKLLWAFEFKNNPAGKNDASAETGSSQGFMHCVKDYDAIVTVRGEERRQ

TILRELEQAQTVFAKYD

 

>CYP619C2  Aspergillus clavatus

          EU678354

          Olivier Puel

          Submitted to nomenclature committee April 28, 2008

          65% to CYP619C1

          ACLA_093640

MDFTQVPPSYILGVLLSSTSILFCLKYLLRSGYRPPELPSGPTT

VPLFGNELQVPKADAHFQFTKWAKQYGGMFSLKRYMNTTIVITDRKLMKSLLDKKSNI

YSHRPASLVSHLITQSDHLLVMQYGEEWRMLRKIIHQYFMEPNCEREHWKVQEAEAKQ

MLHDFLTMPEDHMLHPKRYSNSITNSLVFGIRTATVHDEYMDELFYLMDKWSLVQELG

ATPPVDSFGLLRILPQWMLGNWKNRAVEVGDLMQALYSKVLDQVRARRQRGVYRDSFM

DRVLDNLEKTPLTENQLRFLGGVLMEGGSDTSSSLILTIIQAMTKYPEVQAKAHAQID

AVVGSERSPSWSDFAQLPYINMIIKESHRWRPVSPLGVPHAVAEDDRVNNTLIPKGST

IVLNVWGMHHDPDRWSEPEHFQPDRFADYPALASTYAASGEWDKRDHYGYGAGRRICP

GIHLAERNLFIGVAKLLWAFEFSEPLGSRSDISAESGASQGFLHCPKDYGCAIRLRAP

EKRETIMREFEEAQGVFSRFD

 

>CYP619C3  Aspergillus clavatus

          EU678353

          Olivier Puel

          Submitted to nomenclature committee April 28, 2008

          55% to CYP619C1

          ACLA_093630

MEPMLLLILVAAVVLLFVRWAFVYGHRTSNMPKGPPTLPFIGNI

HQIPTQYTHIKFTEWAAKYGGLYMLKVGNGNMAVVTDRRLVKEVVDRKSGIYSHRPHS

FVSHELITKGNHLLVMHYGDQWRTFRRLIHQHLMESMVDSQHVKIVNAEAIQLVRDYL

VDPEHHMAHPKRFSNSITNSIVFGIRTADRNGSNMKRLYKLMEEWSEIMETGATPPVD

LFPWMKMLPQWMFSNYVNRAKAIGVQMETLYTDILNKVIKRRNGGQNLGTFMDRVLDG

QEKNDLPWHQLAFIGGVLMEGGSDTSSSLTIAIVQALILNPAVQKKAHAEIDAVVGSD

RSPVWEDLEKLPYINMIIKEGHRWRPILPLCFPHALGEDDWVDGKLLPKGTVVVINTW

GMHMDPSQPDDPAAFIPERYANHPQLAPEYAAGKWENRDHYGYGVGRRICPGIHLAER

NMFLAIAKLLWAFEFQRGGGKIDSDPVTGYHNGFLYCAKDYPCRPVVRNKTIRATIER

EFATATKEVFSQFTEG

 

>CYP619D1 Aspergillus niger

gw1.16.300.1|Aspni1

50% to CYP619B2

GPPGAPLIGNILQLPKVRAHQKFTEWARTYGGLYSFRIGPATAAVVTDRALVKELFDKRSALYSSRPTSYVGQNIITRGD

HLLVMDYSDNWRLFRKAINQHFMASMCEKTHVRLLEAEHTQMMRDFLLHPEKHMLHTKRTTNSIIMSLLFGIRTPSWDTP

HMQELYEIMEIWSQIMETGATPPVDIFPWLHWVPQQWLGHWVDRSQTVARGMKRLYSSFHRRAIEARRKAESTSQSRART

FLDDVLDLQEKLGLTDNQVDFLGGVMMEGGSDTGSTMLLVMIQALALHPEIQQRARAELDAVCGEHRSPTWEDFPRLPYI

NMIVKETMRWRPVTPLAFPHALNKDDWVNGYFLPKGTTVFLNVWGLHHDENIFPNPDQFDPSRFEGRHKLAFDYAASPDY

MQRDHYIYGAGRRLCPGIHLSERSMFLGAAKLLWAFNFEPARDEDGNPIRIDTDPVTGYTEGFLVCPRPYQCNVTPRSPA

HAETILREFSRAESEVLSQYAMP

 

>CYP620A1 Fusarium graminearum FG02837.1 AACM01000142 FGcontig1.142_scaffold2

MTGASILYFSALAFTTYLIFKYLQQRSKEPLPPGPKGLPLLGNVLDLPPP

GTIEWIHWQKHKEKYGPISSVSVLGQLFVILHDKQTIMDLLETRSLKSAS

RPKLVFAGDVVGYDSIMGMMPYDRTFRLHRKLTATQVSGKSIGRFEPIQE

LEISRLLKRIYNDHNSDNLPEHLNQVSGSIMLRILYNYETDPNKNDHIVS

MANTVMEEFSKATSPGAWAVDLVPWLKYLPEWIPGAGFKKTAKVFRDHLL

QNVKDPYNYVRDQMARGNDHVSYVAGLVEDIHRKIDPEEESVIQWTAASM

MNAGTDTTGATLLSFFAAMVIYPDVQKRAQEEIDRVVGHSRLPSFADKAN

LPYINSIAQEALRWHTLAPMGFPHMTTENDIYKGYFIPKNILLFPAVASV

THDPDVYHDPMAFKPERYSEPYSEPSPADVVFGFGRRACPGKWIAEQTMF

LIIAQTLATFNIEKDLDKDGREIDVVYEQLPGVISRVKPFPHRIVLRSEV

 

>CYP620A2 Fusarium oxysporum

76% to CYP620A1   Fusarium graminearum

FOXG_15685

MSFSLILVTAIVASLSFFLVKHIQQSKNTPLPPGPKGLPLLGNIRDLPPPGTLEWPHWQTHKEKYGPITSVSVLGQHFVI

LHDRQVIVDLLETQAIKSASRPKLVFAGDI VGYDGVMGLMPYNRTFRMHRKLTATQVSSKSITRFEPIQELEILRLLKRI

YQDQDPKSQNLPDHLNQ VSGSIMLRILYNYETDPCRNDHIVTMANLVMEEFSQATTPGAWMVDFIPWLRYLPDWMPGAGF

KRTAKLFRQHLLQNVQDPYKYVKNQMALGNDNVSYVAGLIKDVHRKIDPEEESIIAWTAASMMNAGTDTTGATLLAFFAA

MLLNPNVQKKAQTEIDRFIGDSRLPSFSDKPNLPYINAISQEVLRWHTLAPMGFPHMTTEDDTYGGYCIPKDTLLFPAVA

SLTHDPEIYHDPMEFKPERYFEPCNEPSPMELVFGFGRRACPGRWIADQTLFLSIAQTLAVFEIKKALDGEGREIEVVYE

QLPGVISRVKPFPHRIVLRSEKYRKFLE*

 

>CYP620A2 Fusarium verticillioides

84% to CYP620A2 Fusarium oxysporum = ortholog

FVEG_13148

MSTSIVIVTAILASLSFLLVKHTLRPKKPPLPPGPKGLPLLRNIRDLPPPGTL

EWPHWQSHKEKYGPITSVSVLGQNFVILHDRQVIMDLLETRAMKSASRPKLVFAGDI (2)

VGYDGVMGLMPYNRTFRMHRKLTATQVSPKSITRFEPIQELEILRLLKRLHQDPNSQKLPDHLNQ (0)

VSGSIMLRILYNYETDSDKNDHIVTMANLVMGEFSEATTPGAWMVDFIPW

LRHIPEWMPGAGFKKTAKLFRHHLLKNVQDPYQYVRDQMT

NGKGDVSYVAGLIKYIHRKIDPEEESIIAWTAASMMNAGTDTTGATLLAFF

AAMVIYPKVQKKAQEEISRATGDSRLPSFTDKPNLPYTTAIAQEVLRWH

TLAPMGFPHMTTEDDTYRGYFIPKNTLLFPAVASLTHDPEVYHDPMEFKPE

RYFEPYNEPSPSDLVFGFGRRSCPGRWIAEQTLFLSIAQTLAVFRIERE

VDAEGREIESRLTKTP*

 

>CYP620B1 Fusarium graminearum FG03700.1 AACM01000161 FGcontig1.161_scaffold2

MGLLLLPAVALAALIVYYVLFPKKQTGLPLPPGPKPLPIIGNLLDLPPAG

TAEYKHWAKHKELYGPISSLNILGQPMIVLNSPDAMHELLEKRSTKTSSR

PSATFGGELCGFKVMLPLIPYGDKFRYFRKLVHQQMGTKLICSEFRDTQD

LESLRFLIRNLERPEELQKHIKTEAAAIILRIIYGYSIEPRKVDPLVSLI

ETMMIHFSDAFVPLSWAVDIFPSLNNLPDWFPGTSFKKTAREWRRVTDQS

LDVPYDFVMKQMSTGTNRPSYVSNLMSKSFKNDQGTGKPTKEDIDAVKAT

ATIMYGGGADTTVSTISSFILAMIKFPEVQKKAQAEIDRVTGGERLPNFD

DEKDMPYIRALCKEALRWMPVVPTTTTHMTEEELEYGGYRIPKGTYLIPS

TWALLHDPEVYHDPSAFEPERYIEPRNEPDPAEYAFGYGRRICPGRYLAE

DSIFMTCVRLLAVFNMRKAVGEDGKEIDVVVDGTPGLISHPVDYAYSITP

RSDKHVELVRAAERAHPWEESDAPSLPKDSLV*

 

>CYP620B2 Nectria haematococca

fgenesh1_pg.scaffold_19000072

Necha1/scaffold_19:183528-185225

56% to 620B1 Fusarium graminearum, 44% to fgenesh1_pg.scaffold_24000005

This gene model seems correct DRN 2/6/06

MFLALLQVFLVLVGIVVWRLSVRWKAPRDLPPGPKPLPLVGNIFDLPLPGVPEYQHWIKFKDTYGPLSSV

TVLGTNMILVHSQEVLQDLIVKRSTKTSSRPKQHFAGELCGFDALTPSLPYNATHRLHRKFMHQQMGTRL

ICERFWHVQDVESRRFLLRVLNDPSNIISHIKT (2)

EASAIILRITYGYSIEPHKVDPLVTLIEHMMGNFSDA

LVPLTWSVDLFPFLKHLPTGFPGASFKKIAQEWRERARMAADIPYSFVLRQIANGNPTSSYVSSLVTEYS

KNPENSWKPTDSEVEIIKNSAAIIYGGGADTTVSTLSGFVLAMLLFPEVQRKAQEELDRVVGSDRLPEFE

DREKLPYVNALIKETLRWLPVVPVGTTHVTEEEMFHSGYRIPKGSFLLPSIWWFLHDPAVYPKPEAFDPE

RFLEPRNEPDPANHAFGYGRRICPGRYLADDSLFLTISRLLATFEINKAVDEKGNEINVEVEVTAGLISH

PNTFPYGIKPRSVRHVEMIRSTESEYPWEEPDVRHLEQDHLQYLHVN*

 

>CYP620B3 Fusarium oxysporum

82% to CYP620B1 Fusarium graminearum

FOXG_09726

MGLLLLPAVVLAALIVYYVLFPKKKTGLDLPPGPKPLPIVGNVFDLPPAGTAEYKHWAKFKDLYGPISSINVLGTPLVVL

NDREALHDLLEKRSTKTSSRPWSPFASELCGYSVFLPVIPYGNKFRYFRKLVHQQMGTKLICSEFRDTQDLESLRFLVRT

IERPEEFQKHIKTEASAIILRIIYGYNIEPKKVDPLVNLIEKMMINFSDAFVPLSWAVDIVPSLARLPDWFPGTSFKKTA

REWRNTTDQALNTPYNFVMDQMASGTNRPSYVSQLMSSKSFKNDNGTGEPTKEDIEAVKATATIMYGGGADTTVSTISSF

VLAMIAFPEVQKKAQAEIDSVTGGERLPTFEDQDRMPYINALCKEALRWMPVVPTTTTHVTEEEIEYRGYRIPKGTYLIP

STWWILHDPEIYANPDAFDPERFIAPRDEPDPSDFAFGYGRRICPGRYLAEDSIFMTVARLLAVFDMRKAVDENGKEIDV

VIDGTPGLISHPVEYAYSITPRSAKHVEMVRAAERVHPWEESDSSLLKKDSPV*

 

>CYP620B3 Fusarium verticillioides

97% to CYP620B3 Fusarium oxysporum

FVEG_08749

MGLLLLPAVVLAAIVVYYVLYPKKKTGLDLPPGPKPLPIVGNVFDLPPAGTA

EYKHWAKFKDLYGPISSINVLGTPLVVLSDREALHDLLEKRSTKTSSR

PWSPFASELCGYSVFLPVIPYGKKFRYFRKLVHQQMGTKLICSEFRDTQD

LESLRFLVRTIERPEEFQKHIKTEASAIILRIIYGYNIEPKKVDPLVSLI

EKMMINFSDAFVPLSWAVDIVPSLARLPDWFPGTSFKKTAREWRNTTDQA

LNTPYNFVMDQMGSGTNRPSYVSQLMSSKSFKNDNGTGEPTKEDIEAVKA

TATIMYGGGADTTVSTISSFVLAMIAFPEVQKKAQAEIDSVTGGERLPTF

EDQDRMPFINALCKEALRWMPVVPTTTTHVTEEEIEYRGYRIPKGTYLIP

STWWILHDPKIYANPDAFDPERFISPRDEPDPSDFAFGYGRRICPGRYLA

EDSIFMTVARLLAVFDMRKAVDENGKDIDVVIDGTPGLISHPVEYAYSIT

PRSAKHVEMVRAAERVHPWEESDSSLLKKDSPV*

 

>CYP620C1 Fusarium graminearum AACM01000001.1 revised

MLLIIVVLVGTLIYFLSFHNKKRHGLPPGPKPLPIIGNIKD

MPPKGVAAFRHWLKHKDTYGPVSSVSVLGQPLILIHDREAAHYLFDKSSG

KSSGRPSANFGGRLCGFDQILSLQQYGDTFKRHRKLVHRQMGTRAGAAKF

RQIQDVESHRFLLRSLDNPGNLMEHIRK (2)

EAGGVILKATYGYSIEPHKPDPLVHLVEFMVEGISIVVVPMKFVVDFLPWLEYI

PECLPGMSFKARARRWRTILNNTIEAPYQFVRQQMAKGIQFESYVSSLLT

QEKLKGGNDTLDETYEADIKRTAAIMYAGGADTTVSTIQSFVLAMMVYPE

VLKKAQAEIDNVIGPDRLPGFEDRENLPYINSMVKESLRWMPAVPMGAAH

KADDDIYYGDLCIPKGSFLLPNVWWFLHNPETYQDPERYDPDRYLEPRNE

PDPDSNCWGYGRRICPGRLLADESIFIVIARVVAAFDIEKDVDEQGNTIE

PKVEFTTEGALSRPVDYPYRIKPRNAKCVDLIRAVEKEHPWDKGDASLLQ

QDMVVL*

 

>CYP620C2 Gibberella moniliformis

DR662816.1 EST

AAIM01003167.1 50% to 620D1, 51% to 620B1, 54% to 620B2

Also Fusarium verticillioides FVEG_10070

2136 MLSAVLILVGVALTILYIRTKPRKNLPPGPKPLPILGNVKDMPDGTIPEYQHWIKFK 2306

2307 DLYGSISHVSVLGQSLIILHDRQAANAILEKTSTKTSGRPQFVFGNEMCGYNQILPFKPY 2486

2487 GKLLKQHRKLVHQQLGTKTAASRFRDVLDVESRRLLLRILETPEKLFEHIQT (2) 2642

2695 EASAIILKMTYGYSLEPRKPDPLALLIEQMMHNFSLAFVPMSWPVDVLPILRYLPESLPGM 2877

2878 SFKRIAREWNANMRMVVDVPYTFVKRQIAKQSNRPSYVSSLIKQHDDDMDEETDAAIKQT 3057

AAVMYAGGSDTTVSAICGFVLAMLLFPEVQRKAQQEIDSVVGTERLPQFEDRDNLPYVDALTKESL

RWIPVAPMGAVHTADEDIHYKDFVIPKGASLLPATWWFLHDPEIYSDPTSFDPDRYLEPR

NEPHPNFASFGFGRRVCPGRFLADESLFISISRLLAAFEIKKAVDGRGNEIEPQISVTPG

LISHIRDFPYDIEPRSEKYAAMMRQVEDDHPWEQADATFLNMDGSLESEKV*

 

>CYP620C2 Fusarium oxysporum (temp CYP620C3)

83% to CYP620C2 Gibberella moniliformis

FOXG_11744

MLPAIFIVIGVVLAIIYIRTRPRKNLPPGPKPLPVLGNIRDMPDGTTPEYQHWIKFKDLYGPISHVSIFGQSLIILHDRD

AANAILEKTSIKTSGRPQFIFGNEMCGYNQILSFKPYGKLLKQHRKLVHQQLGTKTAASRFRDVQDVESRRLLLRILESP

EKLSTHIKTEASAIILKMTYGYNLEPHKADPLALLIEHMMHNFSLAFVPMSWPVDVLPILRHFPETLPGMSFKRIARDWN

ANMRMVVDVPYHFVRKKMAKQSHRPSYVSSLIKQYDEGLDTESEAAIKQTAAAMYAGGADTTVSSIRGFVLAMLLFPDVR

RKAQQEIDSIVGTDRLPQFEDRDNLPYVDALVKETLRWIPVAPMGVAHTADEDIQYKGFVIPKGASFLPAVWWFLHDPAI

YSDPSSFDPDRYLEPRNEPHPNFASFGFGRRVCPGRFLADDSLFISISRLLAAFEIKKAVDGRGNGIEPEISITPGMIGH

IRDFPYDIKPRSEKYADMIRRVELEHPWEEGDAGLLEEELSLYAVNV*

 

>CYP620D1 Aspergillus nidulans AN3394.1 47% to 620B1 64 clan

MTYFLLILTPDSTKMAIASLILWGIGTLICYLIYQHSTRSAQRLPLPPGP

KPLPIVGNL KDFPPDGKPEYQHWIHHKDRYGGISSVTLLGMTLVIIHDKK

AAHELLEQTSGRTSGRPTMVMANKLCGYESIVVCQDYNARFRRCRKFLHQ

ELGTKVSASQFSAAQELEVKRQLVRALNEPEKWLE HFKTT AGATVLKMAY

DYAVDYHKPDPLVDLIDRMMTEFSLAAVPMAWAVDIIPALQYLPGAPFKK

TARKWRKSIQAAAYIP YRFVQSQMAALTYKPSYVSKLVQLLK GEQSELDH

EDEQAIIWSAASLYGAAADTTVITLTTFTLAMILFPDVQRKAQEEIDRVV

GNRLPGFKDREKLPYINALVLEALRWWPIAPMGFPHTATEGFEYNGLYIP

KGAYLLPAVWWFLHDPEVYDNPEIFDPDRFLEPRNEPTPMTEAFGYGRR I

CPGRFFADSSLFLNIAQSLAVFNFKK AVSSDGKEIEIDVKPKPGLLTYPT

KFDFRIEPRSERHIQMIRELERQDPLAAGDAEHLESIDNFQPL*

 

>CYP620D2 Histoplasma capsulatum G217B

ABBT01000228.1 Ajellomyces capsulatus G217B

73% to CYP620D1 but N-term is not detected

HCB07277.1 (fusion to another protein)

Upper part of fusion protein seq is alpha 1,2-mannosyltransferase (deleted)

LPPGA & KPHP

416149 ILGNLK DFPPDGVPEYQHWLKHKDLYGGISSVTVLGLTLVVI

HDRKTAHELLEQTSSKTSGRPTMVMANKLCGYESIVLCQSYTPTFRRYRKYLHRELGTKVSAAQFRSVQEIEVSRQLVRA

LNEPGKWLERRGER (1)

ATVLKTAYGYTIEPHRPDPLVDLIDKMMTEFSLAAVPMAWAVDIIPALR

YLPENFPGATFKNTARRWKKSILDSAYIPYRFVRRQMAGDHTNQPSYVSKLVQQLR &

GEDAKLEHEDEQAIIWTAASLYGAAADTTIITLTIFTLAMIKF

PHVQRKAQEEIDRVVGTDRLPNFDDRSQLPFIDALVKEAVRWWPIAPMGFPHTATTEDVVHNDLHIPQGSLLLPAFWWFL

HDPDVYAGPEAFDPDRFLAPRYEPDPTADAFGYGRRVCPGRFLADASLYLTIAQF (0)

LAAFNMRKAVGEDGREIEVDVRPRLGILTYPTKFDFRIEPRSERH

VQLIRQLERRYPWEPSDAELLESVDDFEAG* 417698

 

>CYP620E1 Aspergillus nidulans AN1601.1 43% to CYP64A1 64 clan

65% TO 620H3 name needs revision 620H3 = 620E4

revised 7/18/07

MAPTAILITVPLGLLLYLLFIRPSIAKKQKAPLPPGPPPKPLIGNLRDLP

SPDQKNWVHFLQHRDLYGPISSLTVFGQTIVILNDARVAFDLLEKRSNIY

SSRPRMVFAGEMVGWEHILAMQPYSDMFRAYRKAMHRVLGTKNVIAQFNE

LQDVEARRFLLRVLEKPGDLVQHIR (2)

TETGAVILKIA

YGYDIEPHGKDPLVALANESLANFAVAGTPGAWIVDTIPF

MKYLPSWFPG

TGFKRTAASWKQTLLTTIEKPYRLVLKQLESGKYPDSYLSNLLEETKGRP

LSADEEQVIKWTAGSLYTGGADTT

TVSTLSCFFLAMALYPDVQRKAQEE

LDTVLGSAKLPTFGDRARLPYIEAIVKEALRWHPVAPMGIPHMSTEDDIY

EGYLIPKNSLIMPNIWAFTHDASHYKDPATFNPSRFLGDTPEPDPSTLTF

GFGRRICPGRLLADSSIFLTIAQSLAVFEISSAGEDAAKAEFLPGVISHP

VPYRLDIRPRSKGHEDLVKRIAAESPFGEGGAKELEEIVV*

 

>CYP620E3 Nectria haematococca

fgenesh1_pg.scaffold_24000005

Necha1/scaffold_24:11514-13534

51% to 620E1, 43% to 620A1 Fusarium graminearum

43% to CYP64A1 Aspergillus flavus

48% to CYP5037B1   Lentinula edodes (Shiitake mushroom) [overlap of families]

MTGLSALILGSAIGVVIVVILKRSFSTA

NGNPPLPPGPNGLPLAGNLNDLPRPGVLEAHHWLKHKGLY (1)

GPISSVTVMGQTLVIINDAQVAFELLEKRSVKHSSRPRQIFVGEM (2)

LGWENS  LGLSQYNDRFRTYRKNMSRIIGSKTAAAQHNTLQEAEVGHFLLHVLDNPEDVVNQIRK (2)

EAGAVILKIAYGYTAEPFKEDVLVNMAGDAMDKFALAGVPGAFMVDMMPF (1)

LRYLPDWVPGTGWKRTARQWAAELHNVTEKPYAFVKHQIA

QGKDDNSFLARLLETGDSTPEEKFTNKWSAMSLYTAGAGT (0)

TVSALACFFLAMSISPDVQRKAQEEIGRVIGKDRLPTLADRPNL

PYIDAIVKEVLRWHPVAPMGLPHTSTADDVCEGYFIPKGSMLFANVW (2)

HFTHDPKVYDEPMSFKPERFLPDDGSEVAPDPYTFVFGFGRRICPGRILADNALYVNIA

QSLAVFDITRGDPVAQPEIR FTPGVVSHPEPFKATIKPRSPHHEK*

 

>CYP620E4P Fusarium oxysporum

64% to 620E3, end badly frameshifted

FOXG_13772

MFEISTFFSGVALGFLLYISIGVIRNGSKRPPLPPGPKGLPLLGNLSHLPPPGVFEAHHWLKFKDLY (1)

GPISSITVMGQTIIIINNWKLASQLLDKRSAKHSSRPKIMMAGEM (2)

VGWENSLGFSPYNDRFRTYRKNMARIIGSKRAAAKYDRLQEAEVAHFLLHVLDDPERFMDHIRK (2?)

EAGSVILKIAYGYTAEPSKEDILIK

MAGQAMDDVTAAGVPGAFLVDILPL (1)

LRYVPDWVPGANFKRLAKKWSSELDDLTEKPYAFVKHQHASGKQDNSFVSRLLEVGDSTE

EARFTTKWSALSLYAAGADT (0)

TVSSISLFFLAMILYPDVQKKAQEEIDGVIGNERLPNCSDRQSLPYVNAIVKEVLRWHPVAPMGLPHTSTVDDVFEGYF

VPKDALIMPNIW (2)

YFAHDPEVHHEPMRFNPERFLSTDGNQPEQDPHMYTFVF &

GVRVCPG & RV & IADMHCFYNI & IQSLAV*HIRKDENDAQP & EL &

LFTLGMISHPEPFKAAITPRSPHHERLIRSVEQEYPWEQSDGHIIENMQRQAS*

 

>CYP620E4P Fusarium verticillioides

84% to CYP620E4P Fusarium oxysporum = ortholog

FVEG_11206

MFEASVFFAGFALECLIYTGLQILGNVSKRPPLPPGPNRLPLIGNLNDLPPPGVFEVHH*LKH*LKFKDLY (1)

GPISSITVMG*TIIIINNWKLASQLLDKRSAQHSSRPKIMMAGEM (2)

VGWENSLGFSLYNDRFRTHRIN

MARIIGSKRAAAKYDRLQEAEVAHFLLHVLDDPERSMDHIRT (2)

EAGPVILKIAYGYTAEPFKEDILIDMAGQAMDNVTAASVPGAFLVDTLPL (1)

LPYVPDWVPGANFKRLAKKWSSELDDLTEMPYAFAKHQHALGKQDNSFASRLLEIEDSTEEARFTTKWSALSLYAAGADT

TVSSISLFFLALILYPDVQKKAQEEIDGVI

GNERLPNCSDWTILPYANAVVKEILRWNPVVPMGLPHTSTEDDV

FEGYFIPKDALIMPNIW (2)

YFAHDPEVHQDPMRFEPERFLSTDGNQPEQDPHTYTFGFGRRVYPGRVL

ADNALFLNIAQSLAVLHIRKDENGAQPKLLFTPGMISHPEPFKAAITPRLPQHEQLIRSLEQEFSWEQSDNHIIEKMQRQAS*

 

>CYP620E5 (old CYP620H3)  Aspergillus oryzae

          GenEMBL BAE55186.1

          55% to 620H1, 56% to 620H4, 65% to 620E1

revised 3/17/2009

MPLPTLGSVLLGLAFLYFVKLVLLRKKTLAPLPPGPKPKPIIGNLRDLPRPGQQEWTHWLKFKELYGPIS

SVSMFGQTIVILNDHQAAFDLMEKRSAIYSSRPRLVFAAEIVGWEDVITLQGYTNRFRSYRKAMHRVLGT

KELMSRFNPLQDVEVRRFLLRILQKPDELIQHIKTEVGAVILKIAYGYNIEPHGRDPLIDLVNDSMENFS

AVVKPGTWLVDVIPLLKYLPTWFPGAGFKRTGYEWRKTLLATIEKPYQLVKQHMRQGSYPPSYLARLLEQ

IDGEPTAEEELVSKWTAGALYAGGADT

TASSLSSFFLAMALYPEVQRKAQKEIDRVVGPNKLPTFED

RDTLPYIDAMVKETLRWHPVGPMGVAHLVTEDDIYEGYLIPKGALILPNIWGFTHDPKIYRDPETFRPER

FLGDNPELDPHTLAFGFGRRICPGRLLADATIFLTIAQSLTVFNFSKPEGEGDLKAEFLPGVISHPAPYR

LEITPRSAAHEALIRSVEVEHPWEESHAKELEKVEC

 

>CYP620E5 Aspergillus flavus

99% to CYP620E5 Aspergillus oryzae

AFL2G_00089

MPLPTLGSVLLGLAFLYFVKIMLLRKKTLAPLPPGPKPKPIIGNLRDLPRPGQQEWTHWLKFKELYGPISSVSMFGQTIV

ILNDHQAAFDLMEKRSVIYSSRPRLVFAAEIVGWEDVVTLQGYTNRFRSYRKAMHRVLGTKELMSRFNPLQDVEVRRFLL

RILQKPDELIQHIKTEVGAVILKIAYGYNIEPHGRDPLIDLVNESMENFSAVVKPGTWLVDVIPLLKYLPTWFPGAGFKR

TGYEWRKTLLATIEKPYQLVKQHMRQGSYPPSYLARLLEQIDGEPTAEEELVSKWTAGALYAGGADTTASSLSSFFLAMA

LYPEVQRKAQKEIDRVVGPNKLPTFEDRDTLPYIDAMVKETLRWHPVGPMGVAHLVTEDDIYEGYLIPKGALILPNIWGF

THDPKIYRDPETFRPERFLGDNPELDPHTLAFGFGRRICPGRLLADATIFLTIVQSLTVFNFSKPEGEGDLKAEFLPGVI

SHPAPYRLEITPRSAAHEALIRSVEVEHPWEESHAKELEKWSVNG*

 

>CYP620E6 Aspergillus clavatus

63% to 620E5, 61% to 620E1 (subfams 620E overlaps with 620H)

ACLA_072970 revised

MESLAVHALLGLTVLYVVNRLFLTKRTPAPLPPGPSPKPIVGNLGDLPPSDAHHWEHWLKHKELYGPISSISVFGQRIVI

LNEAKVAFDLLEKRSVIHSSRPEQVFAGKMVGWENSLAVQTYSDRFKSYRKAMHRVMGSKSVVSEFDSLQDVEVRRFLLR

VLRDPAGLVQHLRTEAGAIILKIAYGYTIDPHKPDILVDLADQALGEFSKAAAPGKWLVDLVPMLKHIPTWFPGAQFKRT

AQEWKARVVATAEQPYAFVRQQMKLGSHKPSYVSSLLEARPLGSHSEEEEWVIKWSAMSLYTGGADTTVASLASFYLAMA

LYPDVQRKAQDELDRVVGCNKLPTFADREQLPYIDALVKEVLRWHTVGPMGLPHTSTQDDIYEGYFIPKGSMFLPNIW

AFTHDPNVYKDPMVFNPERFLGPTPEPDPHTIAFGFGRRICPGRILADRTVYLGIAKSLAVFNFGK

STEDQQPLFLPGVISHPAPYKLNITPRSPEHEELIRSVEIDHPWEESDAAALRNLEY*

 

>CYP620E7 Aspergillus terreus

55% to CYP620E5

ATEG_02682.1

MSTTLLLKAILAIASLYIVKLFLSGKRGAQPLPPGPVPKFIIGNFHDLPPHGVKDWEHWLRHKDLY (1)

GPISSVTVLGQTIVVVNDAELAVELLEKQSAATASRPRLVFASELL

GYGLSLPMQTDLQRTRAYRKAMHHVIGSTKSMADFHPLLEREVRRFLLR

TLRTPKELIQHLKT (2)

LTGAIILEIGYGYTVEPHGPDPFVDLANKVMAEFSVATQPGTWAPDIIPALKYIPSWFPGAEFQRMAKTFNSRVNDFSGKPYAFVQKK

MARGSYKPSYLSRLLEKDNPQPGSEEELVARWSAASLYGGGSDTTLSSLGSFFLAMAMYPDIQRKAQAEIDRVAGNRLPT

FNDRAELPYTEAVVKELLRWLPVAPMALPHRATDDRSCGGYLVPRDALILPNVWGFLHDPEVYHDPMTFNPERFLGSTPE

PDPRKFAFGFGRRVCPGKALAEANAFLAVAMSLAVFRIRDAKQNGRKIDGYPDVTAGVISHPVDFEVEISARSAAHEALI

ESVEKDCPWQEGDGKECDF*

 

>CYP620E8 Aspergillus terreus

72% to CYP620E5

ATEG_07797.1

MALYTLVGTLVALAVVYLLKQLLLSKPPTAPLPPGPKPKPIVGNLGDLPPPGQQEWKHWLQFKKLYGPISSITIFGQTIV

IINDAQVAFDLMEKRSSIYSSRPRMVFAGEMVGWNDALAMQTYSDKFRAYRKVMHRVLGSKAVTARFNPLQHVEIRRFLL

RVLDKPNDLLQHIRTEAGAVILKIAYGYTIEPHGRDPLIDLANESMDKFSVAGTPGRWMVDTIPFLKYLPTWLPGTDFIR

TGNAWRKTLLTTIEKPYQLVLQQMKQGSCPPSYTASLLEEANGNATPEEDLVIKWTAGSLYTGGADTTVSALSCFFLAMT

LYPDVQRKAQEELDRVLGPNRLPTFEDRDNLPYIEALVKETLRWHPVAPTGIPHLCTEDDLYNGYLIPKGALILPNIWAF

THDPNVYRDPASFKPERFLDTDGTAPELDPHMLAFGFGRRICPGRFLADSTIFMSIAQSLTVFRFEKDPAVTAQPEFLPG

VVSHPTPYQLKITPRSAQHEALIRSVEVEHPWEESHAKEIEKIECVV*

 

>CYP620F1  Aspergillus fumigatus Af293

          GenEMBL XP_747154.1

          O-methylsterigmatocystin oxidoreductase predicted

          46% to 620D1, 48% to 620B1, 44% to 620C2, 38% to 620A1, 38% to 620E1

MAYELSTLQLSCVAFVAFMAVLVFRTRTRNLKQNVPPGPRPLPIIGNFFDLPPKGQPEYLHWFKHKDAYG

PVSSINVMGTTLVIFHDKDAAHAVMGKKAQKTSARPQLNFAQLCGFENFLITHQYNDKYRLHRKMVHQEI

GTKGLSAGFRPIQEQESIRFILQTFNRPDDILQHLKTLAAAIVLKITYGYSIERKGQDPLVELIEHAMEN

LSQAFVPLAWAVDSVPAIKYLPDWFPGMSYRKTARKWRAINEAAAELPYDFVKRQMAHKAHQPSYVSNLL

EKHMIKSEDNKINVSAADEEAIKWTAVSLYAAGSDSTVAIIHSVICGLVMFPEVVTRAQEEIDRVVGSDR

LPNFDDRTNLPYVDGIIKEAWRWNPVGPMGLTHKSEEDLVCGEYLIPKGSYLLPSLWWFLNDPKEYPEPR

VFKPERYMEPFNHPDPSEIAFGYGRRSCAGRYFADASVYITVVQLLAVFNVRKARDDQGNEIPVTLQAIP

GMVNRPAPFQFKVEPRSQHHIDLLRRIESEQIPEVSHASLLKPSTV

 

>CYP620F2P Mycosphaerella fijiensis

estExt_fgenesh1_pm.C_10155

62% to CYP620F1

MDYIKIQRSLGY (0)

LAVGILLSLAFLTARVQRLQ

MPLPPGPKPLPLVGNVLDLPPSGVPEFKHWFRHKDKYGPVSSVTLMGLVLVIIHDKEVAYSLLSKKAQKTAARPQFNFAS

LAGFEQFLITHQYNDTYRLHRKMVHHKVGTKSLSGAYRKP (0)

EREATLFVLQVYKNPAARMVHSKT (2)

LAAAVIVKVMYGYEIERDGRDPLCEVIEHAMDNLSQAFVPLKWA

VDAIPAIQYLPDGVPGTGSRKTAAEYAKVNYQAANLPYAFVKQQMQAKTERPSYVANLLKSQAAKHGGMSKLPPEEEGAI

KWTAVSMYAAGSDSTVAIMQSVICALLIHPESVTRAHEEIDRVVGSSRLPTFADRKNLPYVDGIIKEAWRWNPVGPMGLA

HRSEEEMIIKGYRIP*GTYLLPSLWWFLHDPA

TYSDPEAFNPERYLPPLNEPDPS

DIAFGYGRGSCAGRFFADASVYITMVQFLACFDIRKARDANGREIPVKLEPVAGMVNRPKPFAWEVEIR

SAKHTELLEGLKSMLEQLRGDSALLDLA*

 

>CYP620G1  Aspergillus oryzae

          GenEMBL BAE59625.1

          52% TO 620H3, 48% to 620H4, 46% to 620E1

revised 3/18/2009

MMYTLSTLVFLAGILGLYVLLNSKAPRAPLPPGPKGLPIIGSVGSDLPRGGRDWEHWLKHKELY (1)

GPISSITTAGHTHIILNDANVATELLEKRSARYSSRPRLVMANEL

SGSDIFVTTQNEHAIVRALRKRILSQLRSEE

VLLSFYPQVDTLIRRFLLRTLQKPEELIGNIKT

GIGGVILKVVYGYTVEFHDRDPLVDLVGETAVAFGRI

NQPTGYLVDSIPA (1)

LKYLPSWFPGAGFKKEAREYRRGFDTLLNWPFTFARRQMEEGNYEPSFVSRLIE

QRGSLLSLEEEVKIKHAAAAVYQAGYDTTASTITSFFLAMALFPAAQHKAQEEIDRVVGARLPTPEDRGK

LPYVNALINEVLRWNPVAQIGIMHAATEDDIYEGYLIPKGAPIVPNIWAIAHDPDVYSDSMSFKPERFLA

SDGHTPERDPHTLVFGFGRRICPGRPLTDFNNFLTIARSLAVFQVQKATKDGKEIDPIVDYQGGIIGHLS

PFEVSIRPRSAEHEALIRSIEVEDSISRGDSAALESVRV

 

>CYP620G1  Aspergillus flavus

98% to CYP620G1  Aspergillus oryzae

AFL2G_07111 revised

MYTLSTLVLLGGILGLYVLLNSKAPRAPLPPGPKGLPIIGSVGSDLPRGGKDWEHWLKHKVLY (1)

GPISSITTAGHTHIILNDANVATELLEKRSARYSSRPRLVMANEL ()

SGSDIFVTTQNEHAIVRALRKRILSQLRSEEALLSFYPQVDTLI

RRFLLRTLQKPEELIGNIKT ()

GIGGVILKVVYGYTVEFH

DRDPLVDLVGETAVAFGRINQPTGYLVDSIPA (1)

LKYLPSWFPGAGFKKEAREYRRGFDTLLNWPFTFARRQMEEGNYEPSF

VSRLIEQRGSLLSLEEEVKIKHAAAAVYQAGYDTTASTITSFFLAMALFPAAQHKAQEEIDRVVGARLPTPEDRGKLPYV

NALINEVLRWNPVAQIGIMHAATEDDIYEGYLIPKGAPIVPNIWAIAHDPDVYSDPMSFKPERFLASDGHTPERDPHTLV

FGFGRRICPGRPLADFNNFLTIARSLAVFQVQKATKDGKEIDPIVDY

QGGIIGHLSPFEVSIRPRSAEHEALIRSIEVEDSISRGDTATESVRV*

 

>CYP620G2P Aspergillus niger

gw1.10.879.1|Aspni1

60% to CYP620G1 partial C-term seq Pseudogene

IQAKAQEELDRVIGTNLPTTEDRTHVPYINAVIKEVLRWNPVTPLGVAHASTKEDVYEGYSIPKDATMVPTIWAFLHDPK

VYSDPMTFNPERFLTTESYQAERDPHNLAFGFGRRICPGRGFADSTIFLVRSLQAFRVAKIFEDGREIGPVVDYLPGVMG

HPKPFAISITPRSKEHESFIRSIEIEHPWERGNLVLV*

 

>CYP620H1  Aspergillus oryzae

          GenEMBL BAE60302.1

          69% to 620H2, 53% to 620H4, 49% to 620E3,

          48% to 620G1

MMLVVSLLLGLTGLYIIRWTLGERKTLKRLPPGPTSKPVIGNLLDLPSPGTPDWLHWLKHKELY

GPISSVTIFGQTIVILNDRQTVIDLMEKRSGLHSSRPQLPIAEI (2)

TDWDDTLGLIPYNSRFRAYRKALHQEMGTPAS

ILKYHDIIDMETHRLLFRILENPEDLVQHIRK ()

EAGSIILRVGYGYVTEPHARDPLVDLVDKAMEDFSQLV

LPGAWLVNFIPMLKYLPSWFPGNGWQETAKAYKKRVTAMRDVPYTFVQRQIKKQNHVPSYVSSLLEQGNV

EPGSEEEIVAKWSAQSLYGGGAETSVSSLACFFQAMVLNPNVQKRAQEEIDRVVGTSRLPDMSDRENLPY

INAVVKEVLRWHPVTPLGVTHAASEDDTYNGYFIPKGSILVSNIWAIAHDPELYHDATEFKPERFLGVNG

RTPEYDPHLLSFGFGRRICPGQHLAAANLYLAIARSLAVFDITHLVKNGKEVPVTPEFTTGIISHPAPFE

LSIRVRSPEHEKLIRAVEKSYPWEKSHAEELQLKI

 

>CYP620H1  Aspergillus flavus

99% to CYP620H1  Aspergillus oryzae

AFL2G_02999

MMLVVSLLLGLTGLYIIRWTLGERKTLKRLPPGPTSKPVIGNLLDLPSPGTPDWLHWLKHKELY (1)

GPISSVTIFGQTIVILNDRQTVIDLMEKRSGLHSSRPQLPIAEI (2)

TDWDDTLGLIPYNSRFRAYRKALHQEMGTPASILKYHDIIDMETHRLLFRILENPEDLVQHIRK ()

EAGSIILRVGYGYV

TEPHARDPLVDLVDKAMEDFSQLVLPGAWLVNFIPMLKYLPSWFPGNGWQETAKAYKKRVTAMRDVPYTFVQRQIKKQNH

VPSYVSSLLEQGNVEPGSEEEIVAKWSAQSLYGGGAETSVSSLACFFQAMVLNSNVQKRAQEEIDRVVGTSRLPDMSDRE

NLPYINAVVKEVLRWHPVTPLGVTHAASEDDTYNGYFIPKGSILVSNIWAIAHDPELYHDAIEFKPERFLGVNGRTPEYD

PHLLSFGFGRRICPGQHLAAANLYLAIARSLAVFDITHLVKNGKEVPVTPEFTTGIISHPAPFELSIRVRSPEHEKLIRA

VEKSYPWEKSHAEELQLKI*

 

>CYP620H2  Aspergillus oryzae

          GenEMBL BAE61267.1

          69% to 620H1, 49% to 620H4

MLVLVSLVLCLTGFCLLQWALKERKIVKGLPPGPRPKPIIGNLLDLPPPGALDWLHWLKHKELY

GPISSVTIFGQTIIIINGHRVANELMEKRSGVHSSRPHVPIAEL ()

AGWQYTLGFIPYDSRLRAYRRALHQEMGNATS

ISKYHNILDMETHRLLFRILKTPDCLMQHLRKEAGSIILRITYGYITEPEAYDPLIDLVDKAMEDFAQVI

LPGGWLVNFIPML

KYLPSWFPGCDWQRRAKAFKQRAKAMTDIPYAFVKQQHEQQKHIPSYVSRLLEQNNI

KLGSEEELVVKWSAQSIYGGGAETSVSVFACFFQVMALHLNVQKKAQEEIDRVVGASRLPDLSDCKNLPY

INAVVKEVLRWHPVAPMGVAHASSKEDIYHRYVIPKGAILVPNIWAMAHDPDFYHNAMDFEPERFLKSGR

NEQNPEYDPHQFIFGFGRRTCPGQHLVSANLSLGVARVLAVFNITNAVRDGKKVPISPEFSPGVISRPAP

FELSIQVRNAECKRLIEAVGMKFPWEESHAEALAQLRI

 

>CYP620H2  Aspergillus flavus

99% to CYP620H2 Aspergillus oryzae

AFL2G_09608 revised

MLVLVSLVLCLTGFCLLQWALKERKIVKGLPPGPRPKPIIGNLLDLPPPGALDWLHWLKHKELY (1)

GPISSVTIFGQTIIIINGHRVANELMEKRSGVHSSRPHVPIAEL (2)

AGWQYTLGFIPYDSRLRAYRRALHQEMGNATSISKYHNILDMETHRLLFRILKTPDCLMQHLRK (2)

EAGSIILRITYGYITEPEAYDPLIDLVDKAMEDFAQVILPGGWLVNFIPM (1)

LKYLPSWFPGCDWQRRAKAFKQRAKAMTDIP

YAFVKQQHEQQKHIPSYVSRLLEQNNIKLGSEEELVVKWSAQSIYGGGAETSVSVFACFFQVMALHLNVQKKAQEEIDRV

VGASRLPDLSDCKNLPYINAVVKEVLRWHPVAPMGVAHASSKEDIYHGYVIPKGAILVPNIWAMAHDPDFYHNAMDFEPE

RFLKSGRNEQNPEYDPHQFIFGFGRRTCPGQHLVSANLSLGVARVLAVFNITNAVRDGKKVPISPEFSPGVISRPAPFEL

SIQVRNAECKRLIEAVGMKFPWEESHAEALAQLRI*

 

>CYP620H4 OLD name = CYP620E2 Aspergillus oryzae

AP007164.1b CYP64 like

second P450 of six on this accession

seq assembly modified at cyan region

CDS             complement(join(1111497..1111884,1111943..1112214,

1112271..1112515,1112568..1112719,1112780..1112971,1113193..1113425))

53% to CYP620e3

MASLHLASIAIGVLTAYLLTKLLTFKKPPAPLPPGPPPKPIIGN

LKDLPQNGERDWEHWLKHKELY (1)

GPISSITVLGQTFIILNDQKLAVELLEQRSKWHSDRPKMFFAAEM (2)

SGCGGILGLIPYSDRSR AIRKAMN

KEIGSKVAVSRFNALQEAETRRFLLRVLEAPEELRNHIRT EAGAVVLKLAYGYTVEPH

KQDPLVDLADVSMYYFSLVCRYGAWVVDVFPSLR FLPSWFPGTEFKRIGQRSKEAFDN

FGGKPYNFVKHQMSQGTHHPSYLSSILESEEIEPGSEKEYVTKWSAASIYAGGADTTV

STMASFFLAMALYPEAQRKAQEEIDRVVGNSRLPTFADRDNLPYINATVKEVLRWHPV

VPNNLPHLSTHDDMCQGYFIPKGSIVISNIWGFAHDPDVFHDPMTFKPERYLGDNPEP

DSHRISFGFGRRICPGRVMADAAIYLNIAQSLAAFNIGKKVVDGKEVEPRVEFQAALI

SHPEPYDVSIKPRSSVHEELIRAVEEEYPWEKSHADELVNIKV

 

>CYP620H4 Aspergillus  flavus

99% to CYP620H4 Aspergillus oryzae

AFL2G_06042

MASLHLASIAIGVLTAYLVTKLLTFKKPPAPLPPGPPPKPIIGNLKDLPQNGERDWEHWLKHKELYGPISSITVLGQTFI

ILNDQKLAVELLEQRSKWHSDRPKMFFAAEM

SGCGGILGLIPYSDRSRAIRKAMNKEIGSKVAVSRFNALQEAETRRFLLRVLEAPEELRNHIRT

EAGAVVLKLAYGYTVEPHKQDPLVDLADVSMYYFSLVCRYGAWVVDVIPS (1)

LRFLPSWFPGTEFKRIGQRSKEAFDNFGGKPYNFVKHQMSQGTHHPSYLSSILESEEIEPGSEKEYVTKWSAASIYAGGADTTV

STMASFFLAMALYPEAQRKAQEEIDRVVGNSRLPTFADRDNLPYINATVKEVLRWHPVVPNNLPHLSTHDDMCQGYFIPK

GSIVISNIWGFAHDPDVFHDPMTFKPERYLGDNPEPDSHRISFGFGRRICPGRVMADAAIYLNIAQSLAAFNIGKKVVDG

KEVEPRVEFQAALISHPEPYDVSIKPRSSVHEELIRAVEEEYPWEKSHADELVNIKV

 

>CYP620H5 Aspergillus niger

e_gw1.10.144.1|Aspni1

59% to CYP620H4

MWTGAFIAITSVYIFFAVLTRKSKRPPLPPGPRRKPIVGNLWDLPDPSQQDWQHWLKHKDRYGPISSLSIMGQTIIVLND

ARLAVELLESRSSIHSSRPQQHFAEMAGWNNVLGAVKQSQRFRATRKNLHREIGSNVSVARFNEIQTAEVGRFLLRVLDA

PDKLMKHIRKEAGAIILKVGYGYTIEPHDQDPLVDLADKAMMDFSMAMLPATWAVDFFPPLKYLPSWFPGTEFMKIAQRY

RKNVTAFSDIPYAFVKEQMRTGRFVPSFLSNLLESSDLEPGSEEENTVKWSAGSLYAGGADTTVSSIASFFLAMALFPEV

QRKAQQELDTVIGTDRLPQYADREQLPYINALVKETFRWHPVVPMSLTHTSTADDVCEGYFIPKGSSVLANIWAFTHDPA

AYHDPMSFKPERFLGPKPERDPHFLVFGFGRRVCPGRTLADVNVYLTVAQALAVFEISKPVENGKVKDVQPEFLPGVISH

PAPFDVSIRPRSAKHLELLRSLEQKYPWEKSNAEDLK

 

>CYP620H6 Aspergillus niger

fgenesh1_pm.C_scaffold_4000144|Aspni1

60% to CYP620H1, 41% to CYP64A1

MLSIASLLVGLASIYVLYVFLGPKKTSAPLPPGPPPKPVIGNLNDLPPPGSKDWQHWLDHKEKYGPISSVTIFGQTLVVL

NDPDMALEMLEKRSAKHSSRPHLPIADLSGWDKTLGLLPYNNRFRAYRKATYRELGSANAVAKFDGIVDKEVTRFLLRVI

RSPNDLIDHLRKEAGAIILRIGYGYTIEPNARDPLVDLVDKAMDDFSQIVLPGAWLVNFVPFLKYLPTWLPGGKENQIAK

EYKERLTIMHDKTYDFVKNQMARGVHQPSYVSGLLEKDTVLPGSEEEIVVKYSAGALYGGGADTTVSALSCFFMAMALNP

SVQKKAKEEIDQVIGTSRLPDLSNQNDLPYINAVVQEVFRWHPVTPLSVSHASSEEDIFNGYLIPKGAIIVPNVWAFTHD

PTNYRNPETFNPDRFLGAAPEPDPRLYAFGFGRRICPGRLLAEKSIFLTVARALAVLDIRKKVRDGKEVPIRVEFSSGTI

SHPVPFEVDLKPRSKGHEKLVGAVEREFRGRRVMRRSWGSEDMDDWYIAR*

 

>CYP620H7 Aspergillus niger

e_gw1.1.1438.1|Aspni1

53% to CYP620H1

MSLLVRAITGLLGLLILKSFLTRKKFLAPLPPGPKPKPIIGNLLDLPSKGEADWLHWYKHKKIYGPISSVQVFGETLIIV

NDARIAVELMEKRSVIHSDRPSLTFIEMSGFKDIFSMRNNNDPRVRAERKLFYQQIGSNNSVARFNYIQEAEVGRFLVRV

RDNPEGLQDHIRKQAAAIILKLAYGYSVVPHGDDPLVDLIGRAMGNFGAALVPGVWLVDFVPFLRHIPTWFPGATFARVA

ETFRESARAWNCQPYEFVRYQMSQKKHAPSYISGLIEEKGVPEPGSFDEVVMKWSAAAMYGGGSDTATTISTMGGLFLAM

VLYPDVQRKAQEEIDRVVGTDRLPGFDDRDNLPYINAMVKESLRWHSVLPMGVAHCSSEDDMCEGYFIPKGSQILPNQWA

FTHDPDVYPDPMAFKPERFLATENHTPERDPHLLAFGFGRRICPGRTLADANIYLSIAQSLAVYRFTKAIKNGKEVDVEP

KFLTGAISHVAPFEVGIKPRSQQHEALLQALEAKFPWEKGHAEELSQVKF*

 

>CYP620H8 Aspergillus niger

e_gw1.6.1233.1|Aspni1

54% to CYP620H3

MSILTAVAFTCLILLPVYLLINHQIASLPLPPGPKGKPIIGNLSDLPPPDTPEWQHWLEHKKRYGPITSVTILNQPIIIL

HDAQIAIELLEKRSLKYSSRSQTVFVGEMIGWKDTLGMQPYNARFRAYRKAMHQVLGTKALVSKFNTLQELEARRLLRRM

LEDPGEWVQHLKTEAGAIILKIGYGYDINPHGRDKLVDLADDSMETFSAVMNQVWLVDLVPVLKYLPSWLPFSAQRKSQF

WRTQLLTTIETPYRMVREQMASGIAPPSYLSNLLQEEDTKEEKTLTAEEEFTAKWTAGSLYIAGADTTVSALRTFILSMA

LNPAIQEKAQAELDTVLGPHTLPKLSDRDKLPYVNAVVKESLRWYPVGPMGIPHLCVEEDVYDGYRIPKGAIVMGNIWAI

THDPNIYPHPEKFDPERFLGENPQPDPSGWVFGFGRRVCPGRVLADASVFVTVGMVLSAMRISLEKGDGEREEVRFTPGV

VSHPVLAGLKVEARSARHEGVIREVSI*

 

>CYP620H9  Aspergillus oryzae

          GenEMBL BAE60640.1

          58% to 620H7, 56% to CYP620H5

          revised 3/18/2009

          formerly CYP620J1

MSYMVLGLAIGVIFLYFIRSFLARTKSFAPLPPGPRPKPIIGNLWDLPPQGTRDWLHWLKHKDLY

GPVSSVTVFGQTIIILNEARLAYELLEKRSAIHSSRPSCTFAHM

AGYGDIMTILEYSERLRT

TRKVAHQQIGSNKAISRFSHIQDAEVCRYLLRMLRDPGNWLEHIKKQVETGAVILKITYGYTVEPHGRDP

LVDLAEDAVGKFSLAMVPGAWLVDSIPI (1)

LRHLPSWAPGGGCTRAAEGFQTAARNLGNVPYA

FTKQQMTQGSNVPSIISYYLESENIQ

PGSEEEHLVKWATATLYGGGADTTVSTMMCFFLTMALYPHVQRKAQEEIDRVVGATRLPGFEDRGNLPYI

DALLKEALRWHPIVPMGVAHMAMEDDMLEGYRIPKGAAILSNIW

AFTHDPNEYHDPMTFKPERFLSDNGHTPERDPHLLAFGFGRRVCPGRNLADSNLWLTIARTLAAFNIAKPIRDGKEVD

IQPEFQAGLISHPEPFDVDIKLRSAGHHELILAGEKQYPWEESHAEELRRAIAVL

 

>CYP620H9  Aspergillus flavus

57% to CYP620H7, 98% to CYP620J1 Aspergillus oryzae

AFL2G_03370

MSYMVLGLAIGVIFLYFIRSFLARTKSFAPFPPGPRPKPIIGNLWDLPPQGTRDWLHWLKHKDLY

GPVSSVTVFGQTIIILNEARLAYELLEKRSAIHSSRPSCTFAHM

AGYGDIMTILEYSERLRTTRKVAHQQIGSNKAISRFSHIQDAEVCRYLLRMLR

DPGNWLEHIKKETGAVILKITYGYTVEPHGRDPLVDLAEDAVGKFSLAMVPGAWLVDSIPI (1)

LRHLPSWAPGGGCTRAAE

GFQTAARNLGNVPYAFTKQQMAQGSNVP

SIISYYLESENIQPGSEEEHLVKWATATLYGGGADTTVSTMMCFFLTMALYPHVQRKAQEEIDRVVGATRLPGFEDRENL

PYIDALLKEALRWHPIVPMGVAHMAMEDDMLEGYRIPKGAAILSNIWAFTHDPNEYHDPMTFKPERFLSDNGHTPERDPH

LLAFGFGRRVCPGRNLADSNLWLTIARTLAAFNIAKPIRDGKEVDIQPEFQAGLISHPEPFDVDIKPRSAGHHELILAGE

KQYPWEESHAEELRRAIAVL

 

>CYP620H10  Aspergillus oryzae

          GenEMBL BAE64937.1, AP007171.1

          51% to 620H4, 51% to 620H3, 43% to 620E1

          14 P450 genes and 2 pseudogenes on this contig

          revised 3/18/209, formerly CYP620K1

MESILLFLTISLVGLYLVGSHLSKKHSLGHLPPGPPRKPIIGNLTDLPSHDVCDWEYWLKHKDLY

GPISSLSIFGDNIVILNDARFARDILEKRSSIWSSRPSWNFGKM

AGWNKILGTLAYSDPSFKDMRKAIGHQIGSKTAASRFN

AVQDLEVRRFLLRVLEDPDNLLQHIRK ()

EAGAIVLKIAYGYTIEPHGHDPLVDLADEAMATFGLAILP

GTWVVDFIPILKHVPTWFPGAQFARMAKQFRKSAAAFSDVPYAFVKRRLAQRDFEPSFLAGLIRKNEEQP

GPGSYEETVIKWAAASFYGGGSDTTVSTMSSFFLVMAQYPHVQRKAQAGIDCVVGPDRLPSFQDRENLPY

IYAMVKEILRWHPVLPMGTAHASVMDDTYEGYTFPKGTLMVPNVW (2)

AFTHDPSTYPDPHTF

KPERFLSYEGHEPEANPYYLVFGFGRRVCPGRTLADANLYISIAQSVAAFTITKPIRDGKEVDLRAEYQS

GAISHPVPYNVTITPRSPRYEELIRAVETEHPWEKSHSEELRLV

 

>CYP620H10  Aspergillus flavus

99% to CYP620K1  Aspergillus oryzae

56% to CYP620H5, 57% to CYP620H9

AFL2G_05207

MESILLFLTISLVGLYLVGSHLSKKHSLGHLPPGPPRKPIIGNLTDLPSHDVCDWEYWLKHKDLY (1)

GPISSLSIFGDNIVILNDARFARDILEKRSSIWSSRPSWNFAKM

AGWNKILGTLAYSDPSFKDMRKAIGHQIGSKTAASRFNAVQDLEVRRFLLR

VLEDPDNLLQHIRKEAGAIVLKIAYGYTIEPHGHDPLVDLADEAMATFGLAILPGTWVVDFIPILKHVPTWFPGAQFARM

AKQFRKSAAAFSDVPYAFVKRRLAQRDFEPSFLAGLIRKNEEQPGPGSYEETVIKWAAASFYGGGSDTTVSTMSSFFLVM

AQYPDVQRKAQAEIDCVVGPDRLPSFQDRENLPYINAMVKEILRWHPVLPMGTAHASVMDDTYEGYTFPKGTLMVPNVW (2)

AFTHDPSTYPDPHTFKPERFLSYEGHEPEANPYYLVFGFGRRVCPGRTLADANLYISIAQSVAAFTITKPIRDGKEVDLRA

EYQSGAISHPVPYNVTITPRSPRYEELIRAVETEHPWEKSHSEELRLV

 

>CYP620H11 Aspergillus terreus

58% to CYP620H5

ATEG_07254.1 revised

MSPILIATAIGLVAFYVLKWVSTKRLRVALPPGPPRKPLIGNLGDLPSHTDKAWEYWLKHKDLYGPISSLTVCGQDIIII

NDAHIAVDLLEKHSSAHSSRPHATLVEMAGWEDILTSVPYGDHFRAIRRALHQEIGSKVSVARFNHIQEVEARRFLFRVL

DDQKSLVHHIRKEVGAIVLKLGYGYTIEPHKRDPLVDLADRAMEEFSFSILPATWAVDFLPFLKYLPAWLPGAEFKRMTA

TFRKTATAFSDLPYAFTKHQMTQPSHVPSFLSHQLEKGIPRPRSSEEKVAKWSAASLYAGGTDTTVSTMTAWYIAMALFP

DVQRKAQEEMDRVTGGNRLPSYEDRENLPYINAMIKESVRWHSVVPMNVAHVSIQDDSVGEYAIPKGTQIVTNLW (2)

AFTHDPNIYPDPMAFKPERFIDTATHKAERDPYYYSFGFGRRVCPGRTLADANIYITMAL

SVAAFNISKPVRDGKEVEVKLDPQPGIICHPAPVELDIKPRNQVIERLIRAVETDYPWEKSHAELLTSLPY*

 

>CYP620H12 Aspergillus carbonarius

FD661712.1 EST

81% to CYP620H5

TFAGGADTTVSSISSFFLAMALFPEVQRKAQEELDAVVGTNRLPQYEDREQLPYINALVKEVL

RWHPVVPMSLAHTPIKDDTIEGYSIPKGATVLANIWELVKQSN

 

>CYP620L1 Fusarium oxysporum

51% to CYP620E3   Nectria haematococca

FOXG_13173

MESASLIVYTGVITFVFIYVTKLLAKTSHNAQLPPG

PRGLPLVGNVLDLPRAGEFEAHQWAKHKDLY (1)

GNISSITVFRQTLVIINDAKLAQTILNDRSAKHSSRSKMTFAGEM (2)

VGWDKTLSFLPYNGQLRSHRKKAHVCLKSEASIK TNDAIQEIEVGHFL

LHLLRDPDRLVEHIQK (2)

QAGSVIVKVVYGYTAEQFKPDPLLITVRKVVDEFGIAAKPGAFMVDLIPI (1)

LKYVPEWFPGAVFKSTAKQWRSNLESSVEDPAAFVEYQMANGKDNTSFLSQLLQKKS

LTDEEYSENKWLAASLYAAGADT (0)

TVSAITTFFLAMTLFPEAQKKAHYEIDEVIGRDRLPTLSDRQSLPYVNALVKEVLR

WHPVGPMCLPHTTSQDDVIDGYLIPKGAMILPNIW (2)

QICHDSALYHDPMAFRPERFLGPEAETDPGRFVFGFGRRICPAQAMSDKTLFLNMAQTLVVFD

IGVKEGAEMPKAEFTSGVVSHPKPFETEIKPQSSQTRKLIESIERMHPWQQSDAETLASL*

 

>CYP620L1 Fusarium verticillioides

87% to CYP620L1 Fusarium oxysporum = ortholog

FVEG_12380

MELATLITYTCVITPVLICVTKLIAKTSRNAQLPPGPRGLPLIGNVLDLPRAGQFEAHHWARHKDLY (1)

GNISSVTVFGQTLVIINDAKLAQTTLNDHSAKHSSRSKMTFAGEM (2)

VGWDKTLSFQPYNEQLRRHRRKAHLCLKSEASIR

SNDTLQEIEVGHFLLHLLRDPGRLVEHIQK (2)

QAGSVIVKVVYGYAAEQFKPDPLLSTVRKVVDEFGIAAKPGAFMVDLIPILKHIPDWFPGAGFKTTAKQWRTNLESSVEDPAAFVQHQMANGKDNK

SFLSQLMQKKGLTDEEASENKWLAASLYAAGADTTVSAITTFFLAMTLFSKAQKKAQWEIDEVIGSERLPALSDRQSLPYVNALVKEVLRWHPVGPMCLP

HTTSQDDIIDGHLIPKGAMILPNIW (2)

QICHDPALYHDPMAFKPERFLGSEAETDPGRFVFGFGRRICPAQGMADKTLFLNMAQTLAVFEITGKEGGEMPKAEFTS GVVSHPKPFETAITPRSSQHRKLIESIERMHPWQKSDADTLANPHR*

 

>CYP620M1 Mycosphaerella fijiensis

45% to CYP620E1   Aspergillus nidulans

44% to CYP620H5   Aspergillus niger

e_gw1.22.354.1 revised

MIAHPVFAAWLSFVTALILLTIHHVRRVSIKLPPGPRRLPLIENMLDMPPKGGRNWVHYTKQRDLYGPITSLTALGTTVI

LLHDYNLAEEFLSKRAVIASDRPRPFFLTQMHLTIIRCGWGKNIGFIPYDRRLRAMRKQIHALLGTQTALADFNPVQDIE

TRRFLLRVLRKPDQFLKYIKSHRNCSEAAGIMLKTVYGYETNSEGSDPIIFIVEKALEQTSVAGQPGAWLVDYFPALRHL

PDWLPGTGFKRIAKEISRTQNEFSGRPWAFVKYQMAAGKYETSFSSRLLERGEGKLSDEDEYIAKFAANGLYGGGADT (0)

TVASIMTFFLTMIKYPEFRNKAQAEIDHVVGQDRLPELSDRENLPYTEAVIKEIFRWHTIVPEGL

PHLSSEDCEFEGYFIPKGSILVPNIWAFAHDPSIYHDPTTFNPNRFLGDPPEMDPRTFIFGFGRRICPGKLLADSSVFLT

IARALAVFEILPPLDDQDPNFDPEPGFVSHPAAFQARIMPRTEKDRERILEVERTDPFGRGGEEDMERVVVW

 

>CYP621A1 Fusarium graminearum FG05113.1 AACM01000205 FGcontig1.205_scaffold3

I propose a 1 nucleotide MICRO exon agggt for the joint At PK/G/TVV

This is needed to preserve the length and seq in this joint

The phase (1) and phase (2) boundaries do not fit otherwise.

MIELYSFAHMLGPALVFTSLLIFWAHTHFQPTLPLPPGPPSEFLLGHTRV

IPKENAAEVYSRWAKEYSQSTIVXXXXXXXXXLHSAEVAKDILEKKGANFCDRPRFTLL

EVMGWGKTLTFLPFGKSWQMHRKFLQTSFSNTNVRQWYNLQMTETHRTVK

GIMARPDTWETSLRRFAVAIVLQVSYGMEVPSDDDPYIRIANDAMYATGN

GGVPANSLVDLVPFDWLINDWSLRFARQWRWAITKLHDVPFAAAQAER (0?)

CNNKYPANTSLAHQ

LLQSYEHNELQGNKQDWSLDDIKGAAGAVFIAGADTTWATCVIFVLNMVLHP

EIQKKAQDELDAVLRPGKLPEFSDRPSLPYVEHIVQEIYRWSPLAPLGIP

HKSLHDDVYHGMFIPK (1)

G (2)

TVVYANSYAIAHDERVYKSPHE

FNPDRYGAGEPYPVGNFGFGRRICVGRFLAGNSVWIMVATMLSTLQFCKK

VAQDGTPIEPRVQFTNGGTCHPEHFDCVIKPRNATATALINAS*

 

>CYP621A2 Nectria haematococca

e_gw1.5.1476.1

Necha1/scaffold_5:782235-784633

I propose a 1 nucleotide MICRO exon agggt for the joint At PK/G/TVV

This is needed to preserve the length and seq in this joint

The phase (1) and phase (2) boundaries do not fit otherwise.

MEFTTAKALIELAIVLAVMWFALVHRRTQPTLPLPPGPPAEFLLGHTRLIPKENTAATYARWSREY (1)

DSDIIHVKSLGRSIVVLNSVEAARDVLEKKGANFCDRPRFTLLEV  (2)

MGWGKTLTFLPYGRRWQMHRRLLQTSFSNTNVRQWHKLQITEARRTVRNMMGKPSSWETSLRRLAVAIVL

QVSYGTEVPKDDDPYIQIANNAMYATGNGGAPANSIVDLIPL (1)

ARHLPDWIVRDWSLRFARQWRWAIQKLHDVPFAAAQSEH (0)

KLTFDKDGCSDNKSLAHELLRQYRRNEENGQEQEWSLDDIKGAAGAVFIAGADT (0)

TWATCVIFILNMVLHPEIQEKARSQLDSVIGPDRLPNFSDRASLPYIEHIVQEIYR (2)

WSPLAPL (1)

GIPHKSLQDDVYQGMFIPK (1)

G (2)

TVVYANAHAMAHDERIYRAPHDFNPDRYEPLVNGGAGEPFPVGNFGFGRR (2)

VCVGRFLADNSVWIMVATMLATLEFRKKMGPDGSPIEPRVQFTNGGTC (2)

HPEHFECDIRPRSHKAAELIGANHD*

 

>CYP621A3 Gibberella moniliformis

AAIM01001458 79% to e_gw1.5.1476.1

Also Fusarium verticillioides FVEG_06100, one stop codon

I propose a 1 nucleotide MICRO exon agggt for the joint At PK/G/TVV

This is needed to preserve the length and seq in this joint

The phase (1) and phase (2) boundaries do not fit otherwise.

5016 MIEHYLSQALIGLTLAIATVLLFALAHWRSRPTLPLPPGPPSEFLLGHSRVIPKENAAAVYAKWSKEY 5219

5279 DSDIIHVRSLGRSTVVLNSADVARDILDKKGANFCDRPRFTLLEV 5413

5490 MGWGKTLTFLPFGQRWQMHRKFLQTSFSNTNVRRWHTLQITEARRTIQNILKKPETWETS 5669

5670 LRRLAVAIVLQVSYGTQVLEDDDPYIQIANDAMYATGNGGVPANSIVDLVPF 5825

5889 VRYLPDCIVRDWSLRFARQWRWAIKKLHDVPFAAAQAEN (0) 6005

     HRYDYYTNTSLAHH

6132 LLREYKDKESRGQEQQWSLDDIKGAAGAVFIAGTDT 6239

6294 TWATCVIFILNMVLHPEIQEKAQQELDAVIGSDRLPDFSDRPALVYIEHIVQEIYR 6461

6522 WSPLAPL 6542

6601 GIPHKSLHDDIYKGMLIPK (1)6657

6775 G (2)

6790 TVVYANAYAIAHDERVYKNPHNFNPDRYEAGEPFPVGNFGFGRR (2) 6921

6995 VCVGRFLADNSVWIMVATMLSVLRFCKKMSSDGKPIEPRVQFTNGGTC(2)7135

7273 HPEHFDCVIRPRSLATEALVNSD* 7344

 

>CYP621A3  Fusarium oxysporum (temp CYP621A4)

Also Fusarium verticillioides FVEG_06100, one stop codon

I propose a 1 nucleotide MICRO exon agggt for the joint At PK/G/TVV

This is needed to preserve the length and seq in this joint

The phase (1) and phase (2) boundaries do not fit otherwise.

93% to CYP621A3 Gibberella moniliformis

FOXG_08569

MIEHYPSQALFGLTLAIATILLLAWARWRSRPTLPLPPGPPSEFLLGHSRVIPKENAAAVYAKWSKEY (1)

NSDIIHVRSLSRSTV

VLNSADVARDILDKKGANFCDRPRFTLLKI (2)

MGWGKTLTFLPFGERWQMHR

KLLQTSFSNTNVRQWHSLQITEARRTIRDILKKPKTWETSLRRLAVAIVLQVNYGTQVLEDDDPYIQIANDAMYATGNGGVPANSIVDLVPF (1)

VRYLPDCIVRDWSLRFARQWRWAIKKLHDVPLAAAQAEY (0)

HRDDHYRNTSLTHLLLREYKDKEFRGQEQQWSLDDIKGAAGAVFIAGADT (0)

TWATCVIFVLNMVLHPEIQEKAQQELDAVIGTDRLPDFSDRPALVYIEHIVQEIYR (2)

WSPLAPLGIPHKSLHDDIYKGMLIPK (1)

G (2)

TVVYANAYAMAHDERVYKNPHDFNPDRYE

AGEPFPVGNFGFGRRVCVGRFLADNSVWIMVATMLSVLRFCKKMSSDGKPIEPRVRFTNGGTWYIIS

TVPMACFALTHSLQ

 

>CYP621B1 Aspergillus clavatus

42% to CYP621A3

ACLA_013420

Supercontig 76: 1359064-1360557 (-) strand no introns

MESLILLVLIILPASVLIYKRLTPRNLPPSPPGDFFLGHLRRIPSSHAEYQYAKWSRTYNSDILSLRMLTRCVIVVNSVD

AAHALLKKPTAADRPRFALYEIMGWGITLTFLRSSSPRFKLHRRLLQRSFSPSMCKDYRPIQMQEIRAAVAQIRSHPETW

EVSLRRFAIAVVMRIGFGMDSAQAGGFVDLATAVEEATGRGGVPGFSVVDAVPALRWIPVCVAKRVGMLWGLVHAQQMRG

AVEEFHNGPWGMMEEKLRAGTGETGSSFLGMHLREGKVGVEDLKGAAGTIAIAGGNTTFATIVVCILNLMLQPGVQARAR

AELDGVLGVDARGLPLRLPTFEDRERLPFLERVIQETTRWAPLSPLGIPHAMSAEESVGGLTIPRG

AVVYANAWAMTHDE

RVYAEPERFDPDRYLRGEPLPEGPFGFGRRVCPGQHLALTGVYIAMATLLATVSWRCPVDEAGREKRPEVQFSDGLSGVP

DRFECEMQARDAESKEVL*

 

>CYP622A1 Fusarium graminearum FG08187.1 AACM01000329 FGcontig1.329_scaffold5

MDFSQNTTTLGVGDYTPEGAGLVAAGAIILVLSLLVLVSPKEKIPVINKY

PNDWFSIKAQIAFVTNADGLIKQGFAKFQNPFRMITLVGDRLILPEDHFE

WIRRHGPQLDHQPLVRKDFFAGYPGFNGTAAISDPSKLLANVIKKKLVQN

PHVSKLHKSVQRDILAAWP QGCDAWKAVD WAKDGLNLISRMSASVFVGEE

LSHDETWQQVSTNYGMTVFLAARALRQWPRWLRPLIQWFLPACKSCRAEV

KRAREVLEKNLAKRAAEGKEHDDSITWFSEIAAGKIYDPVAAQLGMSMAA

VVTTSELLKQTVIEICSHDLITPLREEIEAVVHEHGWSPAALTNMRLLDS

VIKETQRMNSAVI VNLDRQVLSPVTLPNGQYLPKGTALSIYMSRLRNPEV

YENPDTFDAYRYAKLRAQGGKWTYASSATSTSEDHFVFGIGKPICPGRFF

AVAEVKTAIATILLDYDVRLAEGYTPKLMPFGFELFADPGVQLEVKRRS*

 

>CYP622A2 Fusarium oxysporum

84% to CYP622A1

FOXG_12221

MVQWGVAEPVSGIAILARPSSEPSQPQKIGTVSGRRWSFFLCLDQSKAMDLSKYITPLLAGQYPLGGAVLVAVVAFVLLM

LIAPKDSIPVINKYPNDWFLSKAHMAFITNADGLIKQGFAK (0)

FNKPFRMITLLGDRIVLPADHFEWMRRHGPHLDHQPLVSK (0)

DFFAGYPGFDGTAAISDPSKLLANVIKKKLVQNPHVSKL

HNNVRKDLLAAWPEQSD (1)

DWKAVDWAKDGVSLISRMSASVFVGEELSRDETWQQVSTN

YGMTVFLAARALRAWPRLLRPIVQWFLPACKSCRAEVQRAREVLHKNLAKRAAEDKEHDDSISWFGEIAGGKSYDPVAAQ

LGMSMAAVVTTSELLKQTIIEICAHDLVAPLREEVEAVVAEHGWSPAALTNMRLLDSVIKETQRLNSAVI (1)

VNLDRQVLSPVTLPNGQYLPKGTAISIYMSQLRNPEVYDNPDTFDAYRYVKLRAQGGKWIYASSATST

SEDHFVFGIGKPICPGRFFAIAEVKTAIATILLDYDVRLAEGYKPKLMPFGFELFADPGVQLEVKRRS*

 

>CYP623A1 Fusarium graminearum FG02117.1 AACM01000111 FGcontig1.111_scaffold1

METYLLVAIALAVVFLVNHKTSPKVPSTIPRLQDSSPFLPFKLYWRYLTD

CRRLFLEAYQEFTKDGKTCVLPSMGFKDEIHLAHHHTQWAASQPDGVLSP

FEGFREIDKGDWNSGHASYIQDPWQSVVIKKEMGRFLEILASDVDDELKT

AVPEYVKPGPNDEVDVYESMKWIVAQVSSRFTVGL SLCRDKTYLKDTLEF

ADLFILNSGLLIYTPSLLKPLVGFFITLPTRYNRWKIQKHIQTLYEERTK

RLLNPELYEKSEEPTDNLQMMMKYAIDKHRDQVTLSQISNRLCLANLASF

HQTAVATSNVLINIAA SNAEFNTIEVIRKEMADVLGENGVFDKASVSKMV

QTDSVLRESMRTHGFGNRAMIRKVVSPGGLKTPDGQTLPNGSTMSILSYP

VHNDPTIYEHPEKFHPFRFSQMRSEGNGANLSFVSTSPTYLPFGHGKHAC

PGRFLVDFEMKMILHHVLKEYDIELLPEHNGVRPESHWITEAVMPPVGVK

LRFKKRVLA*

 

>CYP623A2 Fusarium oxysporum

82% to CYP623A1

FOXG_04463 revised

MAVYLFVAVFLAAVYLAQYRSSPKLPSNLPRLERNSYLPFKLPWAYLTDCRQLFLEAYQT (0)

FTKHGKTCVLPSMGFKDEIHLAHHHTQWAASQ

TPDAVLSPIEGFREIDKGDWNSGHANYIQDPWQSIVIKKEMGRFLEILAAAVDDELQYAIPEYIKPGAGDEVDVYE

AMKWIVAQVSSRFTVGLPL (1)

CRDKEYLKDSLAFADLFILNSGLLIYTPSLLKPLIGLLVTLPTRYRRWKIQKHIQPLYEER

TKRLLNPGLYEKGEEPTDNLQMMMRYAISKHGKQVAPSQISDRLCLANLASFHQTAVAISNVLINIAA

SDAEFSTIAVIRKEMADVLAESNGVFDKASVSKMIQTDSILRESMRTHGFGNRAMIRKVV

APGGLKTPDGHTLPNGSTMSILSYPVHQDPEIYEHPKKFYPFRFSKVRTGPDGKDANPTLSFVSTSSTYLPFGHGKHACP

GRFLVDFEMKMILYHVLNRFDIELLPEHNGVRPESQWVTEAVMPPVGVKLRFKKRE*

 

>CYP623A2 Fusarium verticillioides

93% to CYP623A2 Fusarium oxysporum

MAVYLFVAVFLAAVYLVQHKSFQKLPSSLPRLERHSYLPFKLQWAYLTDCRQLFLEAYQT (0)

FTKHGKTCVLPSMGFKDEIHLAHHHTQWAASQ-PDAVLSPIEGFREIDKGDWNSGHASYI

QDPWQSIIIKKEMGRFLEILAAAVDDELQYAIPEYIKPGDGDEVDVYEAMKWIVAQVSSR

FTVGLPL (1)

CRNKAYLKDSLAFADLFILNSGLLIYTPSLLKPLIGFLVTLPTRYRRWKIQKHIQPLYEER

TKKLLNPGLYEKGDEPTDNLQMMMRYAIDKHGSQVSPSQISDRLCLANLASFHQTAVAIS

NVLINISASDAEFNTTTIIRKEMADVLAESGGVFDKASVSKMIRTDSILRESMRTHGFGN

RAMIRKVVAPGGLKTPDGHTLPNGSTMSILSYPVHQDPDIYENPEKFYPFRFSDMRTDPD

GKDANPTLSFVSTSSTYLPFGHGKHACPGRFLVDFEMKMILYHVLSRFDIELLPEHNGVR

PESQWVTEAVMPPVGVKLRFKKRE

 

>CYP623B1 Fusarium graminearum FG09195.1 AACM01000371 FGcontig1.371_scaffold6

MSASVMDLGPELLARLPTLTSPLGIASVAAILLISLFITEKLLSVPYPPS

IPLIREPEGARRFSLRTRWQYLTNCQPMYYEAYHKYLKQGKAVVVPGFGI

RIEVLLPQNQMKWTINQPDEVLDQGLAFAEIDQVSWALGHNRFVVDAWQG

MIVKTEMNRTIEVVANAMKEELREVFDEQFGTDTENWKKIDLTPTIKMII

AQAASRFTVGLPLCRNKEYLQNALDTNELFILNAGLTG

GMPQVFKPFT

GTVFGWLVGSKV

SQLKKWIVPLLKQRVDKIKNHPEEPEPQDLLQMMIKYALRERQDEAENWD

SMTRRVVAQNFGAIHNTQIHVVHLLLNVIGSDAEYNTISVLRDEMDRLLG

SDDTEGWTKAAIQSMTRADSVSRESIRLGTFGTRAVFRKVMTDDFKTQDG

HHLPKGTLLSFNSFQAQTDDELYEDGLKYDPFRFSRIREEAASRGEKAPP

ATFVTTSPEFLTFGHGKHACPGRFLIDFEMKMILAYIVKNYDIKFPDEYE

GKRPPNVWITEANNPPSGTHIMVKRRAQK*

 

>CYP623B2 Aspergillus nidulans AN3497.1 53% to 623B1 54 clan

MHIMNAVDSLLGVGLYFLLPAALLYIINTVFFGAVYPRGVPFIRERPGKT

SFSLKTRLAYLTDCESLFREAYHKYLKNGKPVILPGFGVRTELILPVSSL

RWATTQPDSVLSPGEAFVEIDQADYSLGHSRYVVDAWQGMLVKNEMNAVL

ENIVAAMNEELGVAFDRYFGTDEENWRDIDLLEAAKLVVAQAASRFTVGL

SLCRNEKYLNDSFDAIDGCVITAGVVGGCPPVLRPLIGPIAGLKARLANR

RLRKHIEPLYHARMEALKLPIEDQPQDHFQMMLRFAQRERKEELYSLDQI

ATRVTVANFGSMHQTSIQVTNMLLNILASDPEYNTIAVLRDEIDQVLGSD

TAWTKAKVSKMTKADSVARETLRCHSFGGRAVFRKVLVDGVTTDTGLPLP

KGTLFSFLSQPAHVDEETYEDPFKYDPFRFSRVREAANDPAKAGSLSFVS

TGPSYLAFSHGKHACPGRFLVDFELKMIIAYVLKNYDIRFPEEYQGKRPA

NRWVAEAVFPPSGVRVLVKRRRA*

 

>CYP623B3 Nectria haematococca

e_gw1.12.604.1

Necha1/scaffold_12:172258-173956

MAPLLLDQLPSLTSPLGIVLAGSVTIFVLYLVENLIHVPYPPGIPLIREPEGARRFSLRTRYAYLTDCQALFREAYHD (0)

YIKKGKPVVIPGFGFRKEVILPQSSMKWVLSQPEEVLDVTKAFAEVNQVKWSLGHEKIAVDA

WQGMLVKSDLNRVLENIVNAMNNELKIVFDEQFGKDTENWKEIDLRSTIQIIVAQAASRFTLGLPL (1)

CRNKDYLKTSLKINDLLILNAGLTGGLPLVLQPIVGTLLSIPTGIQFRKLKKWFVPVWKGRMEVDPSLPEPQDH

LQMMLRYGQRERPDEINDMDVMTGRLIAHNFGSMHQTQIQVTNLILNVLGSDAEFNTIAILRDEIDRILG

PDEDAQWTKAFVNQMTRADSLCRETLRLGAFGGRANFRKVMTSGFTTEDGIPLPKGLVISFLGQPAQTDD

ETMEDPLKFDPFRFSRMRELAASRDEKVPLVSMVSTSPEFLPFGHGKHACPGRFLIDFELKMILAYMLRN

YDVAFPEEYEGKRPPNVWMAEAVVPPEGVRIRIKRRTRQSSN*

 

>CYP623B4 Gibberella moniliformis

Genomic seq = AAIM02000051.1 PKS ortholog to PGL1 starting at 437510

There is a CYP623B4 seq at 468-470k 72% to 623B1

94% to 623B4 Fusarium oxysporum

Also = Fusarium verticillioides FVEG_03709

470403 MPYTAE

470385 LGLLARVVSPLGIASIVVIGVIAIYVIDKLTSVPYPPDIPLIREQKGARRFSLRTRWAYL 470206

470205 TDCQALFYEAYHE (0) 470167

470120 YLKKGKPVVIPGFGVRIEVLLPHSQMKGVLNQPEDVLDMEQAFAEVDQVRWSLGHEKYV 469944

469943 VDAWQGLVVKYDLNRAIEIIANNMKDELHEVFDEQFGTDTENWRRIDLTKTVKMIVAQAA 469764

469763 SRFTVGLPLC(2)

       RNKEYLNLALEMNDLFIMNAGLTGGLPCILQPIT  469584 

469583 GTLFGQIVNTKVNKMKKWIMPLLKHRFDRINNHPDEPQPQDQVQMMINYALRHRQHEVND 469404

469403 MESLARRVVAQNFGSIHNTQIQIVNLILNVLGSDAEFNTIATLRDEMDRILGTDDSVNWT 469224

469223 KSGIQAMTRADSVARESIRLCSFGGRAVFRKVMVDDFKTEDGHHVPKGSIISFMGHPAQT 469044

469043 DNEFYEDGLKYDPFRFSRIRETAASRDETAPPVTFVTTSPEFLTFGHGKHACPGRFLIDF 468864

468863 EMKMILAYALRNYDIKLADEYEGKRPPNYWIAEANNPPSGVQIMVKRRERK* 468708

 

>CYP623B4 Fusarium oxysporum

AAXH01000338.1 cont2.338,

PKS ortholog to PGL1

This is the next contig after cont2.337 which has a pks seq at the end

About 31kb away

CYP623B5 = 73% to 623B1 probable pigment modification of pks product

Gene model FOXG_05831

33080 MSSSAELGLLARLPSLASPLGIASIVVIGVIAIYVIEKLIGVPYPPDIPLIREPKGARR 32904

32903 FSLRTRWAYLTDCQALFYEAYHE (0) 32835

32788 YLKKGKPVVIPGFGVRIEVYLPQSQMKGVLNQPEDVLDMEQAFAEVDQVRWSLGHDKYV 32612

32611 VDAWQGLVVKYDLNRAIEIIANNMKDELHEVFDEQFGTDTENWKRIDLTKTVKMIVAQAA 32432

32431 SRFTVGLPL (1) 32405

32355 CRNKEYLQLALETNDLFIMNAGLTGGLPRILQPITGTLFGQIVKTKVNKMKKWIIPLLK 32179

32178 HRFDRINNHPDEPQPQDQVQMMINYALRHRQHEVNDMESLARRVVAQNFGSIHNTQIQVV 31999

31998 NLFLNVLGSDAEFNTIATLRDEMDRILGTDDSVNWTKGGIQAMTRADSVARESIRLCSFG 31819

31818 GRAVFRKVMIDDFKTEDGHHVPKGSIISFMGHPAQTDNELYEDGLKYDPFRFSRVRETAA 31639

31638 NRDEKAPPVTFVTTSPEFLTFGHGKHACPGRFLIDFEMKMILAYALRNYDIKFADEYEGK 31459

31458 RPPNYWIAEANNPPSGVQIMVKRRERT* 31375

 

>CYP623C1 Beauveria bassiana

AM409327

Chembiochem. 2007 Jan 11;8(3):289-297

Biosynthesis of the 2-Pyridone Tenellin in the Insect Pathogenic Fungus Beauveria bassiana. Eley KL, Halo LM, Song Z, Powles H, Cox RJ, Bailey AM, Lazarus CM, Simpson TJ.

Gene name tens2

MALFQAMSMVAQLGYYEKVAGVLGFLSIALLFWKLNHKPFYPAL

PLAGEPPQRRWFSLSNRLRYYNDCAALFDEAYHTAYAKKGKAVLVPSMGVHTAMIMPE

SAMNWAMSQPDDSLSIKKAFSELNQTKYSLGHGRYWEDPWQLDLVKAHLSSILQNLIP

QLNEELAAAFSKHLGTDAENWKEIELEVIMRRIIAQATSRFIVGLPLCRDDGYLDLSY

KVILGMVTTIWATLPFPDLIRAITGPIASWQTRRNIARIQEYLEPLYQERISILESRD

GPESDPEPQDLFMMMLRFAQKKRPDEYANLGIMTRRVCAANFVAMHQSTVSVTNLILN

IIGSDAEFNTTATLRDEITQVMRGTDAKSWTKDTFTRMRKCDSVAREAMRLNFPLGTR

GSMRAVLKDGLESPEGIKLQKGTTISWLASCAQVDADRFDNPQKFDPFRFSRASKDDD

DDGKSTSSHAKDAFVTTSPQYLPFGHGKHACPGRFMVDLMFKILLAQLLTHYDLGWPE

DYQGKQPPSVWQGELSEPPPGARILVKRRKV

 

>CYP623D1 Histoplasma capsulatum

45% to CYP623B3

HCB03238.1 model is short at N-term

MLGFWLESPPDHGLNAAG

FLLSVSTIVLIFITYFAYAHASCGGNIPRLREPSGKKHFSLKTRWAYYTDCAGLFNDAYNN (0)

FSKKGLPVWIPSLGTRNDIL

MPHKDLAWVLAQPPNIISIRHAYNDINGIKRSLGSSKYMLDNWQGMLVSKELNKVLDGIVLSLNDELKYVLESRLGRDTV

NWKVLDLQQTVQMMIVQATSRFHVGKPL (1)

CRNEEYLHLILNIMDEMIFSAAVTGAFPAILQPLINPVASWRLRRLISRVRK

LIEPRFEECMAHDTADDKCTDFFQMLFRFARKERPDELNIWDLTNRLCMANFEAIHQTSMAITNMILNIVSSDAEYDTIS

ILRRETTAHLGCGSGNSREYLDDDTKWTKAKIAKLVKADSIARETMRLHSFANRGLFRKVMADGVQTEDGISLPKGSILS

FLSQPLHTDGDIFGDPLKFDPWRFSRAREQCESATASPATVEAAGTHSLVSTSQLLSFGRGRNACPGRFLVDFEVKMIVS

YLLLKYDIRFPEEYGNQRPQNRWLAEASVIPKDARVMVRRRK*

 

>CYP623E1 Neosartorya fischeri

51% to CYP623B2, 46% to 623B4

NFIA_042090 revised

Note: this seq does not have an ortholog in A. fumigatus

MGASTFSQSFAGGYEAWASLMLPALVGCALLIYRAFFAIKYPANLPLAGEPEGKRTFSWRTRWRYYTDCEALYRETYEN (0)

YTKSGKTVLLPGLGFRNDIVLPQSA

MRDIMARPETELSHADAVLELVQLKYALGHEKYKADPWPCMLVKSDINSKLEAVCDGMNEELKYVFDKYIGCDTESWKEV

DLLETIRTVIMAAASRFTVGFPLCRNEAYLRACWKVNDGIVMNGGLTGATPRLLRPIVGPLITMKLRRSIEQVKKQVEPI

YHQRVQALSQQNSAEKPASEEPQDLFQQMLCYAQRERPGELHDLPSMSRRLCFANFAAVHQTTLLVTNMILNIVSSDSQY

NTISVLRDEVNDVIGPDSDTKWTKYKVAQMIKSDSVARETMRLYSNTNRGVFRKVLVEGVKTEDGIELPKGAYVSFLGRP

LQCDPEAFEDPFEYDPFRFSRIREQAPRDTKGRSSASHLSFVSTSPEHLPFGHGGHSCPGRFLVDFEVKMIVAYLLMNYD

IEFPAEYKGQRPANRWMAEALVPPSGARIRIKRRS*

 

>CYP623E1 Aspergillus terreus NIH2624

AAJN01000124.1

93% to CYP623E1 = ortholog

ATEG_04418.1

39131  MGASTFSQSFAEGYAAWALMLPALVGCALLIYRAFFAIRYPANLPLAGEPDGKRTFSWR  39307

39308  TRWRYYIDCEALYKETYDN(0)

       YTKHGKTVLLPGLGFRHDIVLPQSA  39487

39488  MRDIMARPEKELSHADAVLELVQLKYSLGHEKYKADPWPCMLVKSDINSKLEAVCDGMNE  39667

39668  ELKYAFDKYVGCDTESWKEVDLLETIRMIIMAAASRFTVGFPLC

39845  RSEAYLRACWKVNDGIMMNGGLTGATPRLLRPIVGPLVTMKLRQSIEQVKKHVEPIYRQ  40027

40028  RVQALSQQNSAEKPASDETQDLFQQMLRYAQRERPGELHDLPSMCRRLCFANFAAVHQTT  40207

40208  LLVTNMVLNIVSSDPQHNTISVLRDEVKDVIGPDSNAKWTKYKVAQMIKSDSVARETMRL  40387

40388  YSNTNRGVFRKVLVEGIKTEDGIELPKGAYVSFLGRPLQCDPETFEDPFEYNPFRFSRIR  40567

40568  EQAPRDTKGRSSASHLSFVSTSPEHLPFGHGGHSCPGRFLVDFEVKMIVAYLLMNYDVEF  40747

40748  PAEYKGQRPANRWMAEALMPPSGARIRIKRRS*  40846

 

>CYP624A1P Fusarium graminearum AACM01000117.1 Gibberella zeae PH-1 revised

MISDYLQLPKGYEIPILFLTPILLNFVGTILYNVFFHPLAAVPGPLACKV

CRAWLYLAERGGDGANTVAALHKRY (1)

GPLVRIGPNEVSIKDREAFVTINQK

GADF*K

DPAFYDAFSSNHGNLFTFSNVDEYS

KRKRLMSPSFSRASTEKHDAIVHATITPLVEEVALSVRKGESIDLYSVMR

RFAIKSIISFCYGNNSISPDYFESVIPKLFQVLDESPKDLLVLQHFPLLR

KSLTHLATVFPQLFPESIKFLQMVGMELLSSSRKSSSATQPGLFKEMQTL

LKVKDQSLTDDELIAESSTMFFAGTDTTATTVSVALWHLIHQPDDYARLQ

DELRTIMPDVNSGPSLRELESLPFLEACIKESLRLACPVRGRLPRIFPPE

GLEVNGFRIPARTVVSSCISYMTYDEEVFPEPWKFRPGRWLQDNSKDLDG

FLYPFSRGTRSCIGQSLSLAEQRVAISQMVRRFSPRKGMQFREIVGKELF

QTWVNTEKVNQVPVGDDSTR*

 

>CYP624A2  Fusarium oxysporum

80% to CYP624A1P   Fusarium graminearum

FOXG_06966

MLPDYFQLPKKYEILFLFLSPILLSLFGTVLYNVFFHPLATVPGPLACKISRVWLYLAERGGDGANTLAALHKRY (1)

GPLVRIAPTEVSIKDREAYVTINKQGSRFLK

DLAFYDAFSSNHGNLFTFSNVDEHSKRKRLMSPSFSRASMEEHDTIVHATIKPLVEEVVVSVRKGETVALYSVMRRFAIKSIIAF

CYGNNLISPEYIDNTVIPKLFQVLDKSPKDLLVLQHFPIVRRSLRHLATTFPQLFPEAIRFLQRVGMELLDSSRESSDAS

QPGLFKRMKELLKVKEQSLTDGELIAESSTMFFAGTDTTATTASVALWYLMHQPDNYARLQDELKTVMPDVNSRPSLREL

ESLPFLEACVKESLRLACPIRGRLPRVVPPEGWEVKGVRLPGGTTVSSCISYMTYDEDIFSEPMKFRPGRWLQENSKELD

GYLYPFSRGTRSCIGQSLSLAEQRVAISQIVRRFSPSKDMQFREIIGKEYVTWVMEDELPVQLKEAE

 

>CYP625A1 Fusarium graminearum FG10991.1 AACM01000457 FGcontig1.457_scaffold8

MPLILSITSSGTVLVLLTLLSLAAVVILSTYRLIKYRHIPGPLLCRVSHH

YVTLFDLLRQRPQTVAKWHRQYGPFVQIAPGQVSISDVTAMRELYSSSAR

NPKSGYFDNFLYHNARAIFAEKEYLGHREKRGLVSSFFQATSVYKPDIQQ

PLRDRVLAAMMRIEENIITSGQGVVDVMPIINHYAWDNTTSLVFGPCHSS

QALQGDENDRNLLARLKNSEMWGAVKNNLPIVFSGIKLAVAVYTRSTKYF

SAEDDLDKWAMQRLYKTTSDPKSSQDERSIVQLIQHLRQNGRPLSDSYLE

SEIIDNLYAGQATVTVALTYAVYHLSRNPYWQSMVQRELDGLPRDADGLP

NWTLLNKAPILEACIRESYRLNPVASGRAERVLARDSRYGDIFIPQNTIV

SASTIALHLDPQVWLNPREFNPRRWLDATPDEILRLERSFIPFGYGARLC

LGKAFANLQIKMFVAAIYSKDNTGLEIPGQTMEQWGTQDALPKGLKCRLR

FEERK*

 

>CYP626A1 Fusarium graminearum FG11465.1 AACM01000467 FGcontig1.467_scaffold9

MSVTIVSAIAIFFVAIALKLLSNFYYGPLSSLPGPCYTCVTGIPLLYSRA

TGTSREHLRCLHKRYGPVVRVGPTEVSLNSVDGYYKVHGVGSHCIKAPVF

DSIRFSHSPMLFTIRDPKFHAQRKRILGRGLAAMKEGQESKIRRLAELAV

SNIEKEVQEGKSDVYKWWRCLAVDVISEMSFGKPFELLRPGGQDLPVYKA

LGNAGSGVVLQVMLPSFLQSLVRWSPITWLRDIARVGETIFGRVLIALNE

ASLPSCGPSMVRHILGEAELSKSPVLSDEELGSEVSMMLVAGSDSTAATL

TYATWAIIRDPDLRRKIEEEVARLPEHFTMKDLDRLPLLNSTLEETLRLY

NPAGALVERVLPPSGMSIHGWDLPGGTMVYTTGWLISRLEDVFPDPDMFD

ATRFIDPSPEQTRAHVPFSIGSRSCIGMQLARMEILVTLATLFRQCRDMKL

HHGMTDEMM

 

TQFAPPPPRRSSGGGFLGLSTWQVIALLIILILLLLGIFM

CMRKKSEPKAPEEDEGEEEEEEEEEEEEGDEEAADDDEGEEEPVPDEMQM

NAYNAQYLANPYGNPYAQQPSSATIIGSHAIYTFPRGFAVEWLFIVVNDS

IAKGFQVDNQLSAMDSDPWTDAMPGGDQTGLFGAERARRTQ*

 

>CYP626A2 Nectria haematococca

fgenesh1_pg.scaffold_58000026

Necha1/scaffold_58:57207-58707

59% to 626A1 Fusarium graminearum

MSATVILTIVVLALIFLFKILFDYSQSSVSSLPGPWYTRFTDIPFLYARLVGTSRHYLQRLHKIHGSVVR

VGPKDVSINSIDGYYKIHGVGSHCTKTPVFDKIRFSHSPMLFTMRDPQAHAQRKRILGRSLTAVRADQET

TIRRLVELAISQIKTEAEDGRVDVYKWWRCLAVDVMTEMAFGQSFDLLESGGQDSPVSKALEKVNMKVML

QAILPEALMSPLRWSPITWVRDVVCCTEVMFGHVMAALGELRHSPKCATTMLGRILSEDNIKSRRAALDD

DEMGSEAAMMIVAGSDSTAATLTYAVWEVLRHPVLRQQVEDEVAALPLGFTAKEVEALPLLSNVLEEVLR

LYNPTGAPVERSVPPGGISVDGWDIPGGTTVYTQSWLLSRLEGVFPQPEK (2)

FDANRFANPSPELKRAHVPF

SIGSRSCLGMNLARMEIRLALALFFRECRGARLHHSMTDDMMTQVGEFFIVPKAGRCDISVP*

 

>CYP626A3 Fusarium oxysporum

67% to CYP626A1 Fusarium graminearum

FOXG_13273

MFLSFIPILSIVLLAFVLRALFNYHRGPLSRLPGPWYTHFTGLPLLYTRAVGTSRQHLRHLHKVHGPVVRVGPKEVSINS

VDGYYKVHGVGSHCLKAPVFDHIRFSHSSMLFTMRDPRIHSERKRIIGRGFSSIKEEQEVKIQRLASQAVANIKKEAEKG

QADVYKWWRCLAVDVVSEMSFGKSFNLLHSGGKGLPLYTALSNAGPSVVFQAVLPRRLISLFKWSPIAWLRDVGQVTETI

FNRVTSALGELRVSSNCGPSIARHLLSQEVKGKKPSLNDDELSSEVSMLLVAGSDSTATTLAYATWQIVRDPDLRKQIEE

EVSNLPTNFTAKDVEGLPLLNSALEEVLRMHNPAAALVERLVPPSGISVHDWDIPGGTMVYTTGWLISRLEDVFPEPDR (2)

FDATRFLNPTPEMKRAHVPFNIGARRCVGMHVARMEILVTLAMLFRECRGLQLHHGMTDEMMTQVGEFFIVPRAGRCDLSV

 

>CYP626A3 Fusarium verticillioides

90% to CYP626A3 Fusarium oxysporum = ortholog

FVEG_12256

MFLSLIPILSIVLLAFVLRAAFNYYRGPLSPLPGPWYTHFTGLPLLYTRA

VGTSRQHLRHIHKVHGPVVRVGPKEVSINSVDGYYKVHGVGSHCLKAPVF

DHIRFSHSSMLFTMRDPRIHSERKRIIGRGFASMKEEQEVKIQRLASKAV

ANIKNEAEKGQADVYKWWRCLAVDVVSEMSFGKSFNLLHSGGKGLPLYTA

LSNAGPCVVFQAVLPRGLISLFKWSPVTWLRDVGQVSETIFNRVTAALGE

LRSSSNCGPSIARHLLSKEVKGKTPSLNDDELSSEIGMLLVAGSDSTAAT

LTYATWEIVRDPQLRRQIEEEVGSLPVNFTAKDVDILPLLNSVLEEVLRM

YNPAGALVERLVPPSGISVHDWDIPGGIMVYTTGWLVSRLEDVFPEPDR (2)

FIATRFLNPTPEIKRAHVPFNIGARRCVGMHVARMEILVTLAMLFRECRG

LKLHHGMTNEMMTQVGEFFIVPKAGQCNIGI*

 

>CYP627A1 Fusarium graminearum FG07804.1 AACM01000320 FGcontig1.320_scaffold4

MWSKPLPGHLAQVADSLPPEVTEALSNLTWTQGFVAFVALFIIVPRVFEL

LRNILSPVSRIPGPLINKLSPWPLEIATFKGKSHRFARALHQKYGPIVVL

APNMISVGDANEIKRIIQNEDWVKCEAIYGNFRQDPHRPTLLAFTEKKAY

SRRKRMLSSMFGIRYIRSLEPLMKSCVDAGVAHLDKLCENSNNSTVINLQ

HFIHGLAIDTIGATTFGGSFNVVENGSHPLPSRLKAGMKISAVMQLISWI

KYIPFLPKRDPYIEEFTFNIVDKRRKESGDVKHQDLLQHLVDVSDDSPSS

EFRTSDVQDESVILLAAGSETTANAELFTIMQLLKHPNIMKKLVEEVDKW

YPPSEPDRDTECAYSQAGMTYLQACIDETMRLIPGQATGSPRDASKQETV

LGYRIPKGTTVFPNTQEAHLNPDNWENPDQFIPERWLDIYSQNQTSSVPY

WPFSAGSRVCVGKHFAFQEMHISLTTLLRKFSFEYVPGQDETTVFRIAQQ

LQANSYDVKVKRRFV*

 

>CYP627A2  Fusarium sporotrichioides

          GenEMBL AY226098

          Brown,D.W., Proctor,R.H., Dyer,R.B. and Plattner,R.D.

          Characterization of a fusarium 2-gene cluster involved in

          trichothecene C-8 modification

          J. Agric. Food Chem. 51 (27), 7936-7944 (2003)

          gene="P450B"

          94% to CYP627A1

MWSSKPLHDNLAQVASSFPPEVSETLSNLTLTQGFLAFLVLFII

VPRVFELLRNIFSPVSRIPGPLINKLSPWPLEIATFKGKSHLFARALHQKYGPIVVLA

PNMISVGDANEIKRIIQNEDWVKSEAIYGNFRQDPHRPTLLAFTEKKAYSRRKRMLSS

MFGIRYIRSLEPLMKSCVDAGVAHLDKLCENSNNSTVINLQHFIHGLAIDTIGATTFG

GSFNVVENGSHPLPSRLKAGMKISAVMQLISWIKYIPFLPKRDPYIEEFTFKIVDKRR

KESGDVKHQDLLQHLVDVSDDSPGSEFRTSDVQDESVILLAAGSETTANAELFTIMQL

LKHPHVMKKLVEEVDKWYPPSEPDRETECAYSQAGMTYLQACIDETMRLIPGQATGSP

RDASKQETVLGYGIPKGTTVFPNTQEAHLDSDNWKNPDKFVPERWLDIYSQNQTSSVP

YWPFSAGSRVCVGKHFAFQEMHISLTTLLRKFSFEYVPGQDETTVFRIAQQLQANSYD

VKVKRRSV

 

>CYP627A3 Nectria haematococca

e_gw1.8.77.1

Necha1/scaffold_8:382363-383945

MASQPSPIVGRLKLPESMPSLVSLEELTATKVLVTFLTIVIIVPRVFT (0)

VIKNAFSPISSIPGPLLNKLSPWPLTIATIKGTS (2)

HHFARSLHEKYGPIVVLAPGMIAVADTKEIKRIIQTEDWTKSEAIYGNFRQDPQRPTLLAYTDKKAYSKRKRMLS

SMFGIKYIRSMEPIMMTCVEAAVRQLNKFCDEGSQGAAVVDMQHLIHSLAIDVIGITTFGGSLHVVDNGS

HPLPSRLKAGLRIAGLMQLIPWIRFIPFLPTRDPYVDKFTYDIVDGRRQELESSQHSDLLQKLVEASDDS

PGSDFRTSDVQDESVVMLTAGSETTANAELFTLMMLLKNPEVMNKLVDEVDQWYPPSEPDRQTDCGYSQA

GMAYLQACIDETMRLVPGQATGSPRETSKDDVVLGYRIPAGTTVFPNTQESHMQDAHWEEPQKFVPERWL

EAQAANMPYWPFSAGSRVCIGKHFAFQEMHLTLVTLLRKFKFEYVDGQDESTVFRVAQQLKAESYRMKVSKR*

 

>CYP627A4 Fusarium verticillioides

83% to CYP627A2 Fusarium sporotrichioides

FVEG_11079 not found in the F. oxysporum genome

MESTPLQYPLGLKTDSLVKQVSEVLKSLTVTKAVGAFIVLFIIIPKVF

DFLRNLFSPVTSIPGPLINKFSPWPLEIATFKGKSHRFARALHRKYGPIVVL

APGMISIGDSKEIKRIIQSEDWVKSEAIYGNFRQDFHRPTLLAFTEKKAYS

RRKRMLSSMFGIRYIRSLEPLMKSCVDAGVAHLNKLCDNPSKSTIINLQ

HFIHGLAIDTIGVTTFGGSFHVVENGSHPLPSRLKAGMKISAVMQLISW

IKYIPFLPKRDPYIEKFTFDIVDKRRKEAGAVKHQDLLQHLVDVCDDSPGS

EFRTSDVQDESVILLAAGSETTANAELFTVIQLLKHPEKMKKLIAEVDKW

YPPSEPDRATECAYSQTGMTYLQACIDETMRLIPGQATGSPREASKQEVL

LGYKIPRGTTVFPNTQEAHLDGSIWEQPEKYIPERWLEIYSRNQTSAMPY

WPFSAGSRICVGKNFAFQEMHISLTTLLRKFTFEYVPGQDETTVFRIAQQ

LEADSYKVRVKKRF*

 

>CYP628A1 Fusarium graminearum FG03086.1 AACM01000147 FGcontig1.147_scaffold2

MAGPQAYSPSDEQCEVMEPPTMTFSSISLDSAESLAILFFLGVASHVLAF

RKGEWDMWTMPFICTWITYQAVTPCALNQLRHMAYFDAMILANKWLFSFL

FGMTSSILIYRGFFHRLNKFPGPFVARLSNVYASWLAIKEEHMYLEVQKL

HQKYGDIVRIGPQELSIATPSAFRILHANNSPIVKGPFYNVARPCVNLLA

DRNKKSHAQRRKTWDKAFTAKALRDYEPRVVRYTKQLTEQIEKTKGNPIN

IGAWINFYTFDIVGDLAFGTSFNYLVNGVKDKFLRDSHDSQVLMGYFRQC

TWLFEVFKETPLLNNSWLSFQSWLKQKVEARRQNKPSEPDVLSWILEEYD

SIENPTKDDYLRLCGDAHLIAVAGSDTTSAATSILLHQLTLHPHVLKKLQ

AEIDEYQANYEKPDHFSMTKLQYLQACIDESLRLHPVIMSGLQRMTPPEG

MQIDDVFIPGDTIFHAPSYTIYRDERCFVRPLEFIPERWTTQPELIIDSS

VYAPFSTGRGACAGKQLGLMEMRYVLTEILSKYNMSFAPGTNPEAFIDGL

RDCFTLELPELNMIFTPRGEKSHQTVPY*

 

>CYP628A2 Nectria haematococca

e_gw1.35.73.1

Necha1/scaffold_35:40906-42850

53% to 628A1 F.gram. GKMILTMLL seems long

MDLLDPFKSYCVVFLVGVLLHVVFFCRGEWDIYTNKIVVAWVGTLAASTFLIAESAEYESDSHSEAFTIA

LKHCSTLAAGTICSILIYRAAFHRLNVFPGPFKARLSNLYVTALGLKKRHLYKEVQELHRKYGDIVRI(1)

GPSEISINSSEALHALHSNNTPCIKGPWYNIIYPMVSLLADRNKKSHTERRKTWDKAFNAK (1)

ALRDYETRVVQYVGLLLHQLEKMEGQPFNAKLWIHYYTFDIMGDLAFGQSFGMLRDGLKHYYMKLGDENMSL

IGAFSRLIWLFPLFKAIPGVNYTHLKFQRWLREQVDRRRKASEFRL (0)

PPVSDVFSWILEDYESKDQPTKQDFLNLHGDAHLIVVAGS (2)

DTTAVTTTSLFYELSQRLDICSQLQEEIDEYKKEHKKSDYASLSHLKLLQACID

ETLRLHPVVPSGLQRMTPPEGLQIGDTFIPGDTIVQVPSYTLQR (1)

DERVFLRPNEFLPERWTSQPDLVKDASAFAPFSM (1)

GRNSCVGKQLGLMEIRYVTTEILSRYDIQFAPGNDPKAYLENKMDVFTAAVPDLNLVFTPRI*

 

>CYP628A3P Fusarium oxysporum

51% to 628A2, 48% to 628M1, pseudogene

FOXG_10725

MHPTSLAIGTGLSSGILAHILIFSKGEWDLYTIKIL

QGLLIVIVLLALSFKRLGTTEAGSPYQRLRI &

TIASIYMVSLMVLGTFCNIFLYRICFFHRL*QFPGPLMAKVSNLYLTRRSVARFQLYKEI

QNLHRTYGDIVR (0)

(1) VGPSALSILDAKAFHAIHANNSPCRKGPWYNIEQPA

ISLHMTRDKNDHSRRRRAWDRAFSSK ()

ALRDYEPRVVKYTSQLLNRLEQSQATTIDIAKWFKFYSFDTMGDLAFGQSFNMLTDGVKH

PFMALVESHMAMAGTFSQLIWMFPLFRAMPFLGREDAIFQKWIGNQVRHQEQ (0)

NEPDLPNIFSWLLEDYKSQLYPKEQDWLSLQADMQLIAVAG

SDTTSVTLTCLFYLLATNKNACMRLQEEIDNLFSSSSQPNHSSFSKLTYLQTCIDETLRL

FPPVPSGLQRMTPAEGLQVGEIVIPGGTIVTVPSYTVYR ()

DERYFTAPNDFVPERWMIKPEMVKDDSVFAPFSV

GRYACVGKQLGLMEVGYATCMILHRFNICLFGEGTITKPAFLKGLRDHFTLNAPELNVILTARKH*

 

>CYP628A3P Fusarium verticillioides

83% to CYP628A3P Fusarium oxysporum

no gene model

MYPTSLAIGTGASSGVLAHLLIFRKGEWDLYTIKILQGLLMVFGLLALSFRRLGATEDGSPYSLLDPS &

IASIYMVSFMVSGAFCSIFIYRISFFHRLYKFPGPFMARAFNLYLTKRSVASFQLYREIQDLHRRYGDIVR (0)

GPSALSILDAKVFHAIHSNTSPCSKGPWYN

IEQPAISLHMSRDKNDHARRRRAWDRAFSSK ()

ALRDYEPRVVK

YTSQLLDRLVQTQDIPIDIAKWFKFYSFDTMGDLAFGESFNMLTGGVKHPFMALVESHMA

MAGSFSQLIWMFPLFRAMPFLGRADAIFQKWLENQVRHQEQ (0)

NKPDLPNIFSWLLEGYKSQIHPKERDWLNLQADMQLIAVAG ()

DTTSVTLTCLFFLLATNKDACVRLQEEIDNLFSSFSQPNHSSFSNLTYLQACIDETLRLF

PPVPSGLQRMTPAEGLRVGDIFIPGDTIVTVPSYTLYR ()

DERYFTSPDDFVPERWTTKPEMVKNESVFAPFSV

GRYACVGKQLGLMEVGYATCMILNRFDIFLSGEGPTSKSAFLKGLRDHFTLDALQLKIVLTARK

 

>CYP628A4 Fusarium oxysporum

62% to CYP628A1

FOXG_12115

MLWTEAHLETPQAFGAAFLLGVLVHVFVLRKGEWDL

WTVKLIKAWATYEVTVSLFLAQLYSLSVWQALSVTNKWFASFATGLTISILVYRAFFHRLN

RFPGPFLARLSAFYPTYLVVDEEHMYLEVQKLHEKYGDIVRI (1)

GPTEISIATPSSFRVIHATASPVTKSPFYGISHPWTNLLAERRKRQHANRRKAWDRAFTTK (1)

AIALKDYEHRVLKYTKLLTDRIDDMKGKPFNVSLWVNFYTFDIMGDLAFGKS

FNMLENGVEHKFFTESHKSQGLLGAFRRLIWFFPLVTAIPIINSSYLSFQAFIRNQVETRRK (0)

DTPEEPDVFTCILEDYDAIEKPTEQDYNNLCGDAHLIVIAGS (2)

DTTSSSTTCLLHNLALHPDVLAKLQAEIDDYKNTHEESDLISLGKLQYL

QACIDESLRLYPVVPSGLPRMTPPEGMMEIDGVYIPGDTILHNPSYTMYR (1)

DERCFEKPNEFIPERWTTKPELIKDASVYAPFST ()

GRGVCAGKQLGLMEMRYVLMDILSRYDIAFAPGTNPQAFIDGLRDCYTLELPRLDMVFTPRASENGKA*

 

>CYP628A4 Fusarium verticillioides

86% to CYP628A4 Fusarium oxysporum = ortholog

FVEG_10685, FVEG_10685 join

MLLTEAHLETRQAFGAAFMLGVLVHIFILRKGEWDLWTVKLIKAWATYEVTVSLFLTQLYSF

SVWQALSVTNKWFTSFVTGLSISILTYRAFFHRLNRFP

GPFLARLSTFYATYLTVDEEHMYLEVQKLHEKYGDIVRIGPTELSIATPSSFRPIH

APSSPVIKSPFYGITHPCVMLLSERRKKEHALRRKSWDRAFTTK

ALRDYEHRVLKYTKLLNDRIDEAKGEPFNIALWVNFYTFDIMGDLAFGKSFDMLESGV

EHNFFTESHKTQGFMGAFRRLVWFFPLVSSIPIVNSSFLAFQ

AYIRNQVETRRKNKPAEPDVFSSILEDYDAMEKPTQQDFDNLCGDAHLIVIAGS (2)

DTTSSSTTCLLHNLVLHPDVLAKLQAEIDEYKNTHDEYDLVSLSKLQYLQACIDESLRLY

PVVPSGVPRMTPPEGMQLDGVYIPGDTIIHSPSYTMYR (1)

DERCFEKPNEFIPERWTTKPELIKDASAYAPFHT (1)

GRGVCAGKQLGLMEMRYVLMDILSRYDIAFTPG

TNPQAFIDGLRDCYTLELPRLDMVFTPRTGGNAKA*

 

>CYP628B1 AAIM01002625.1 Gibberella moniliformis 7600

47% to 628A1

same contig as CYP5055A2 about 16kb apart

also Fusarium verticillioides FVEG_04308

7029  MITLSNIQLESLSALCCVALCGVATHLAIFRIGEWDVAVHRIIASFSLIYASIFGLLAYYGDFERQ

6851  SLLNAWYIASELLAAFIAGLYGSMVLYRFFFHPLRQFPGPTFAPLSALCAISMSMKKFHM  6672

6671  FLEVQKLHKTYGDFIRL (1) 6618

6558  GPSEISVASPSAVATIYQNSSPCKKGPWYNVFVPTISLHATRDRQEHTLRRKVWDRGL SAK (1) 6376

      AMRDYEPRIEKYTGLLMNQLQQRCGRPVDITDWCGFYGF  6205

6204  DVMGDLAFGKSFNMLNDGVKHYYMELTQMSTLWGSPIGRASWLYLLVKDIPILNRQIVQFLK 6019

6018  WLRKHVDQRTK (0) 5986

5933  NEPHLPDLFSWLLGAYKEQSVHTKQDDLNLLGDAHLIVVAGS (2)

5756  DTTSTTMTCALFELARHPQVYQKLQNEVDEFMKQGDSPHSHSALAKLKYLQAIIDETMRL 5577

5576  YPAIPSGLQRITPPQGLQIDGTFIPGNTIIQTPTYTLNR (1)

      DERCFVRPNDFIPERWTTQPDLVRDASAFAPFSI (1)

      GRYSCAGKQLGLLE 5217

5216  VRHVLAQIASKFNIRLAPNQTVEAFQEGLADGFTLLCPKLEMVFEARVS* 5067

 

>CYP628B1 Fusarium oxysporum (temp CYP628B2)

80% to CYP628B1

FOXG_17440 REVISED

MMNLSAIQLDSFPVLVVVAFCGVATHLAIFRIGEWDVAVHRIIAFFGLIY

AFIFTLIAYHRDLDHQSLLSAWYTASALLATFIAGIYGSMVIFRFFFHALRRFPGPTFAP

ISSLYAVSMSMKKFHMFSEMQKLHNTYGDFVRL (1)

GPSEMSIASPSAVAAIYQNSSPCLKGPWYNVFVPSISLHASRDKQEHAHRRKVWDRGLSAK (1)

AMRDYEPRVEKYTHLLISQLQKRCGKPVDITEWCNFYGFDVM

GDLAFGKSFNMLNDGVRHYYMDLTQTSTLWGTQIARSSWLYLLIKSIPILNYQIANFLKW

LRTHVDQRAK (0)

NEPDLPDLFSWLLGAYREQSVHTKQDELNLLGDAHLIVVAGS (2)

DTTSTSLTCALFELSRHPDVYRKLRTEVDDLLKPEDTHSHSALAKLKYLQAVIDETMRLYPAIPSGLQRITPPQGLQISG

TFIPGNMIVQTPTYTLNRDERCFVRPNNFIPERWTTQPELVRDASAFAPFSIGRYSCAGKQLGLFEIRHVLSHIVSKFDL

RFAPDQTSELFQDGLADGFTLLCPKLEMIFETRKNGELPN*

 

>CYP628C1  Aspergillus oryzae

          GenEMBL AP007162 also BAE61392.1

          47% to 628A1, 45% to 628B1

MDTLWVLAFSSGVATHLLLYRSGEWDIKAPSIVKIYTLLGATLVYLERADLL

DGFPVSTRPKWGIAVILYHIFGVYAS

MLFYRAFWHRLCGFPGPFLARLSNFYVTSLSAKRLHLYEEVQKLHQQYGDYVRL GPTELS

IADPQAVKALYSGQAKVTKGPWYTVLEPRVSLQMSRDKKEHARRRKVWDQGFSSK (1) 891591

891531 ALRDYEPRVSHYARQLLEAVR

KNVGKPMDMAKW

FNYYSFDVMGDLSFGKSFNMLAGGQDTYFSTQLHADMKSIGLFSHLTWLFPFFKRIPILNKDYLKFWDWV

GGRVEERIK (0)

891143 NEPDRPDVFSWILDAFQNGPKTKQDHLDLHGDAYLIIVAGS (2) 891021

890959 DTTAATLTNLFFHLAADHTWQAKLQEELDALPDLTQEKVTNVKLLDALINETLRLH

PAVPSGTQRLTPPEGLQIGDKYIPGDVMVCIPTHTLFRDERAFVRPDEFLPQRWMTQPELVKDASVFIPF

NAGPYSCVGKQLALMELRRVTAEILTRYDVEFAQGQTTEDFLDG

KRDTFTLVTAPLKLVFRER

 

>CYP628C1  Aspergillus flavus

98% to CYP628C1  Aspergillus oryzae

AFL2G_09748 revised

MDTLWILAFSSGVATHLLLYRSGEWDIKAPRIVKIYTLLGATLVYLERADLLDGFPVSTRPKWGIAVILYHIFGVYASML

FYRAFWHRLCGFPGPFLARLSNFYVTSLSAKRLHLYEEVQKLHQQYGDYVRL

GPTELSIADPQAVKALYSGQAKVTKGPWYTVLEPRVSLQMSRDKKEHARRRKVWDQGFSSK

ALRDYEPRVSHYAKQLLEAVRKNVGKPM

DMAKWFNYYSFDVMGDLSFGKSFNMLAGGQDTYFSTQLHADMKSIGLFSHLTWLFPFFKRIPILNKDYLKFWDWVGGRVE

ERIKNDPDRPDVFSWILDAFQNGPKTKQDHLDLHGDAYLIIVAGSDTTAATLTNLFFHLVVDHTWQAKLQEELDALPELT

QEKVTGVKLLDALINETLRLHPAVPSGTQRLTPPEGLQIGDKYIPGDVMVCIPTHTLFRDERAFVRPDEFLPQRWMTQPE

LVKDASVFIPFNAGPYSCVGKQLALMELRRVTAEILTRYDVEFAQGQTTEDFLDG

KRDTFTLVTAPLKLVFRER*

 

>CYP628D1 Aspergillus niger

gw1.4.1072.1|Aspni1

36% to CYP628B1, 38% to gw1.13.785.1s, 39% to CYP628C1

C-term is short

ILGITAHLTYFIHADLEKHVVFLVNCLIFWPSLGLIALFILGYRDISQWIGPAILSFHFSLLASIAIYRYFFHALDGFPG

PKLARITALWDFKNTVLNAKWYIKVKEMHGVYGDVVRIKPRELSINDPSAIKDIYGVGTTCVKGPFYDLHYPHRSLQMAR

DKAFHSKRRRLWDRGFTSKXALAGYEPYLLEHCKDIVRVIESRSSQAQEATALIDGFAWDSMGIFAFGKSFNMLHGEPPK

MLQMMRMMGRGASALLSSSWLVILMRGMVGVRRFTDRWLEWCAQCVEERSRVETNRRDLFSYLIEELSSEGDQSIGGVDG

DLVRDSELAITAGSDTAASTLNALFYLLARHPDKLRRLQEEIDTAVPADEELSHAALVKKPYLEGCINESLRLCPAVLSG

LQRETGPEGLRTAGVYIPPGMIVSVPTYTVQRDPRNFPRPNEFIPERWSTQPELVIHKEALNAFSSGTYSCAGKAFAMME

MRLLVSTIVRHFDIEFPPGE

 

>CYP628E1 Histoplasma capsulatum G217B

ABBT01000077.1 Ajellomyces capsulatus G217B

43% to CYP628D1

C-term seemed too long so removed a possible intron

HCB02359.1

MLQVVCTAGTLLGIISHLLIFKHGEWERYTPTLTALYAFAIGLLSPAVTLTLTVLSIPSTLLLVSAFWVSYMTGLFGSIG

LYRGWLHPLRDFPGPLAWRLTALWSIKVSVPEFKFPVKVQQLHEEHGDFVRIRPREISINHADAIRDIHGPGTVCVKGPF

YDLNYPFRSLQMLRDKGDHARRRRIWDRGLGIKALTTYEPKILGHCADLINQLIARSRNGKPIEIGPWMNYFGFDVIGDV

AFSKPFDMVKNGKAAQILETFEMSRPIAGSLVCVPWTFILLKRLPVLRDVWSARVKDHSRKVVERMKLGTACKDLFSYLL

DDSPGSPATSAPFVFPEFSVPGDLVCDSELAITAGSDTVAATLTALIYILATHPDKQALLQQELDTLLTGIDDISYQKLS

136153 IPNGSPMLEGVILETLRLYPGAPGGMQRMTPPEGARIAGRWIPGNTLVSTPTYTLHR (1)

DPRNFEHPTTFIPERWSPAAANLIKHKEAFNPFLIGANSCAGKAMALMELRLVTALIFRAFTVRLHA (0) 136645

KPGWRDYFTAKPPPFEVLLERRA*

 

>CYP628F1 Fusarium oxysporum

51% to CYP628C1 Aspergillus oryzae

FOXG_17036

MMMVEPKSFEAIVVSFASGCLLHILFYRKGEWDLLALRIFQAYATLPIIVIGLLRLAEFKGWQHDT

NLSAVSVLLLEGAHILGLLTSILCYRMIFHPLNKFPGPLRARISNFYPTYLTTKNAHLYE

EVEQLHQKYGDFVRL (1)

GPTQLSIIHPKAIDAIYSAKSPCTKGPFYNILHPRVSLHMIRDHKEHSNRRKIWDRALSSK (1)

SLRDYEPRVTKYTTQLLDRLNEMQGTTINASNWLNFYSFDVMGDLAFGKSFNMLRDGVN

HYFLSSLHDSMKMVGALAHISWIIPILKLIPAVSAEDRKFWAWIESQVEERSR (0)

MKPDNPDVFAWILDEYERSPKTHQAKLNVEAEAYLIAVAGS (2)

DTTSATLAGLLFELATNPSQIIKLRDEIDMYFSDREHADHTSLSNLIHLNAVIDESLRLHPPVPSGLQRVTPPQGLMVGDTFIPGNTIVQVPMHTVQR (1)

DERYFVRPSEYIPERWTTSPELVKDKTVYAPFSI (1)

GRYSCVGKQLGLMEVRYVIAHIVRAFDVRLGEGQTQEGFLKSKMDTFTLATPNLHLVFTPRLTK*

 

>CYP628F1 Fusarium verticillioides

91% to CYP628F1 Fusarium oxysporum = ortholog

FVEG_13835

MMVEPKSPEALAVSFASGCLLHILFYRRGEWDSLALRIFQTYATLPIIVIGLL

RLAEFQGWQHGNNLSAASVLLLEGAHILGLMTSILCYRTVFHPLNKFP

GPLSARISNFYPTYLTTKNTHLYEEVEQLHQQYGDFVRL

GPTQLSITHPKAIEAIYSAKSPCTKGPFYNILNPRISLHMIRDHKEHSTRRKIWDRALSSK (1)

SLRDYEPRVTKYTTQLLDRLNEMQCTTINASDWLNFYSFDVMGDLAFGKSFNMLRD

GVNHYFLSSLHGSMKMVGALAHISWVIPILKLIPAVNSEDRQFWAWISGQVEERSRMKP

DNPDVFAWILEEYERNPKTYQAKLNVEAEAYLIAVAGSDTT

AATLTGLLFELATNPSQIIKLREEIDQYFMDREHADHTSLSNLIHLNAVIDE

SLRLHPPVPSGLQRVTPPQGLMVGDTFIPGNTIVQVPMHTIQR (1)

DERNFARPSEFIPERWTTSPELTKDKGVYAPFSI ()

GRYSCVGKQLGLMEVRYVIAHIIRAFDVRLGEGQTPE

GFLQAKMDTFTLATPNLHLVFTPRPTK*

 

>CYP628G1 Fusarium oxysporum

no gene model

66% to CYP628G1, 47% to CYP628F1

upstream = NmrA family transcriptional regulator

MTTLYNPINLAYHGIAFALGVILHLSVFRRGEWNIHALSILQYFAIFEGIFT

CALRLFLGSEKCTTWTVIPIALTATLSTLVGLFTSMLIYRGFFHPL

KGYPGPFFSRFSSLYITFQAFKNRRLFEELQLFHKTYGDIVRI (1)

GPNELSILGPHALQALHSTSSSCTKGPWYSIEHPVTSLQMTRDKEEHTRRRRVWDMAFSSK (1)

CLRRYEDRVAEYTTQLLQHIEALQDEPLDISMWFNFYSFDVMGDLAFGKSFDMLS

NGTKHPFMELTHSHMLVAGSLSRLAWIFPLLKRISIFNQKTCELEERIKQLVDWRIK (0)

NEPDVPDIFSWILRDYFGLSKLTGQETLNLYGDAQLIAVAGR (?)

SDTTAASLTCLFFELAINPQALHNVQEEIDRYHLEYSTLDHQNLSKLSYLQACINESMRL

YPLLPSSLQRITPPEGLQVGSIRLPGDTIVSIPSYAFNR ()

DERLFKNADQFIPECWTTMTELTRDSSLFTPFSI (1)

GKYSCVGRQLGLMEIRFVASQILRVFDVKLANCNTAKEFASGLRDSFTLA

CPSLHLVFTRRDIWTPSPISEK*

 

>CYP628G1 Fusarium verticillioides (temp CYP628G2)

66% to CYP628G1, 51% to CYP628A3P Fusarium oxysporum

FVEG_05671

MAALHAHPYPAYYCLSFVL

GVLAHLFVFRRGEWNLYVFNILQAFAVLESILVYIVARAVEGGDSPVWKVTAISSCFTLS

ALMGSSISMLIYRGWCHRLSRFPGPFYAKLSNFYITFHAFKRFRLFEEVQQLHRKYGDIVRI (1)

GPSELSIIGPRALQALHSNSSPCTKGPWYSIEHPIKALQMTRDKEEHAYRRKAWDMAFSSK ()

ALREYEARVAGYTTQLVEQIEASQGTPIDASSWFNFYSFDVMG

DLAFGRSFDMLKNGTSHPFMELVHSNMLMAGSLTHLPWIFPLLKRIPVLNRKNLEFQGWL

KQQVDWRHK (0)

NKPDLPDVFSWILSDYDALNKPTAQDTINLRGDAQLIAVAGR (2)

SDATAASLACLFFELAANPEACLHLQRELDQYY

ADHGKPDHSTLSKLRYLQACINESMRLYPAIPSGLQRMTPPEGLDVGSTHLPGDTVVTIPTYTFNR

DERLFTHADKFIPERWTTKKELTKDPSLFVPFSI (1)

GQYSCVGKQLGLMEIRFVASQILLKFNVELTHQDVARDFVAGLRDGFTLAMSSLHLVFTRRTT*

 

>CYP628H1 Aspergillus terreus

46% to CYP628A2

ATEG_08441.1

MLISLDGSCILAAAYILGIGLHQTIFRYGEWDLHSFKILVAFSVVPIFLGTFLQSFHLPSRGKTTWESLEYSSVLVFIAI

CGIFSSISVYRGWFHRLNEFPGPFLARFSTFYMTFVNIAEARGFKTTQALHSKYGDIVRVGPRELSVANPEAFRAIHLSS

SCGRGPWYNVTAPMISLQMERDVKRHGERRRAWDKAFDNR (1)

ALGDYMTRVNAHTENF

CAAIEDSVKNNTSLNVTKWIHLYSFDIMGDLAFGSSFDMLQSGTFHYFFNLTHKTLELIAIFSRLVWLFPVVKAVPFLSN

DYKSFRKWVGSHVQRRIAVPPPVRDIFSWILNDYQRKQNPSWQDTENLYGDASLIVIAGSDTVAGTLICLLYHLASSPDV

CKKLRDELHSCAKERADTELRPDELAKLPYLQACIDETLRLWPAVVDGLQRETPPEGLTIGTEKKSTWIPGNVIIKTPSY

TLCRDPRAFSRPDEFIPERWTTEPELILDASVYAPFSLGRFSCVGKRLAMVMMRRFISSIILRYELEVLFKSAQDAFERQ

VKEYFTLTPPDLQIRFIKGQKG

 

>CYP629A1 Fusarium graminearum FG11424.1 AACM01000467 FGcontig1.467_scaffold9

AACM02000392.1 Gibberella zeae

missing the C-term

MEAGGIFELRVRLYWFQAFCTSSLKQFRDLERVHIRQCLALRVDLRN

SDIPGPFAASFTRLWHMNHILKGDQNLELIRLHEKLGHFVRVSYDEVSVSHPD

AISKILLSPLHKLTLLEGNWYKIHALPDYRFQSPMSTTDPRKKVKKSRYI

AGAYSVSNVVRSEEHIDRTFEQFLEWMNRYANDKKPIYLDKFISYTTFDV

IGEIVFSKQFGFLEQGIDIGGAIRNSLALNAYVAVAGYFRWINVLLVANS

LVTWMGIMPMGHLFNTTAAVLANREKNHDVRYDAVSYWFYQHSQHPDKLT

IREINTQALAAVGAGSDTVAAGIQSFIYHMIRHPNAWARAQTEVQDATKK

GLCEDNIVSYADVQELPYVQACVKEALRMFGPVPMGLPRIAPRGGLTFGD

RTIPEGTIVSVSPWVIHHSKEYWGEDAHEFNPDRWLKGDAV (0)

176062  FGAGYGSYPGQNVAKIELAKITATLVRDYNIQQVDPKQEWSWKAYFTVVPHSWPVLVEKIR*  176247

 

>CYP629A2 Histoplasma capsulatum

ABBT01000202.1 Ajellomyces capsulatus G217B

58% to CYP629A1

HCB05376.1

MAGLKEFSGLGGPQLAGLFTVAVLTLCIWRRYLSPI

SDIPGPFAASFTRLWHIHRILKGDQNLELIRLHNQHGHFVRVAPNEVSVSHPDAVRKILGTMLHKDKWYKI

IAFPDGRFENPMSATDPKVKLELSKHLAPAYTLSNLLINEPSVSETIKLLLGWLDKFASSGEPIDLGKYFTFATSDIVGD

AVFSKQFGFLREGRDINNTIANATPQTAYVSVAGYFRWFHVLFLSNPVITWLNITPWGHLIDTAMTAIKERQKNPDARID

AVAHWFRMLNQHPDRMKLHEIHSAAFNAVAAGNETVSSGLQAFVYYMIRHPNAWDRARAEIDAAGLKDPVISYMEAQKLP

FLQACIKETLRIFGPAPMGLPRIAPEGGLTIGDRTIPQGTVVSVSIWVMHYSKEIWGPDAGEFNPDRWLGEDAARLTKYY

IPWGMGYASCPGQHLARIELYKICAALVRDYNIRQVDPKQEWTWKAYFTVVPHNWPCIVEKRNKETTP

 

>CYP629B1 Fusarium oxysporum

45% to CYP629A1 Fusarium graminearum

FOXG_16432, supercontig_36 275364-279696 region

MIQINSVNSEVLVSWPGFITELALLAFAIAVHRRYFSNLSDIPGPFWASVTRLW

HVWIILEGKQNVRLKALHRKHGQFVRIAPNEVSVCHPDGSTLLLRANLHK (0)

GDWYSVTAVPDYRFQNPMSTTNPKMKKALSKHFTAGYALSQVLKREPDVDE

NIEHLLRWVEKYAEDRMPMDLDRFITYTTFDNIGSALFSEPIGFIRA (0)

GRDIDGTLRNNVALSRFAAVAGFFIGPLRILINPLNSWLLLLPMGRLYKTTSVAI KKRLHNHEAHNDMVAHWLGAQQKSGELSLRKIEAQANVNVGAGAEPVSHVV (2)

SFCIILIQHPRRLALGFVTRIHRRESLKARAMVASVSFHDADNLTYLRACIKEALRVFSP

TTMGLPRVVAKGGINIAGRHFQQGTILSVSSH (2)

AVHSSKDIWGDDADQWNPERWLSGDTKK (2)

QFSAGYMTCPGRHFAWMQICKMAATLLRNYNIRQVNPKNQWRYQANFTALTHSWPVWVEK

REHEGNMHPDIETLPRDRDQF*

 

>CYP630A1 Fusarium graminearum FG00012.1 AACM01000001 FGcontig1.1_scaffold1

MIVFYSLGAAFAALFVRIFIYPVILYFADPKGLRKFENFSWLSPLTDLRH

CYLSHTGKRSETLAAEHERRGESILRIGPNALSFNHPQAVRDIYGHQTKC

TKDHKETVLAGPHRNLFDVVDKGEHSRKRRLLSAAFATRNLAKWEFKVRY

TTQRLLDAFDPLCTAPLKTNTDPDPEDLTVDFNNWINLWTIEAINYIALS

SKMTLLDTGTDEAMAETTDGTLYPARYRQSQNHSAMVKSVFCWEYELYPW

LRRISKVVPNKWKSYWATSAPWVDVCYHQAKNRLRRYQAGEKLDDFFSCL

MHDKNGVANAVEWGEVVAEISAIIDAGAETTAIALTHLLQLLISHPAQLQ

KLRDEISGVIGEDDVIAPLDKVKDLTYLKACLDEGLRLIPPVSSGLPRRT

PPEGANIMDTWIPGNTSVSMTIWSAHHNEDIYPDAYAFKPERWLDPTERK

RMEPFFIPFSAGARGCIGRNISYLEQQIVVSTLVHRYDFAMPTKDFKVSR

FEAFNLIMGQLPIKIWRR*

 

>CYP630A2 Nectria haematococca

e_gw1.26.42.1

Necha1/scaffold_26:302810-304387

59% to 630A1 no introns

This gene model seems correct

MLGYIALGVVLALAFAYLIVKPIVVYFLDPKGLRKYPNYALLSGITDFRHCYTSSQGFRSRSLYE

AHKNTGEPILRIGPNSLSFGDTRAVKAIYGHNTKCIKDDNYNVLSGSHRHLFDVVDKPDHSRKRRLLSAA

FAIKNLEKWEYKVAYTAKRLFEAFDKKCTAPLKGSIPDPQDVNLDFSHWVNLWTTEAICYITLSAKMDLL

DTGTDEVPAEKRDGTRYRGRYRDARDQNALAQAVWVWDYNLWPWVKWLTKTIPGKYQKMWDAAKAWDDIY

YHQAAERLRRFEAGEELDDFFSCLMEDKTGSPNNLEWGEIVSEIGAIINAGSDTTAIALTQTLELLIKHP

QHLQHLRQELDSVLEPDEAIAPYDKVKDLPFLKACIDESLRVIPPTSAGLPRRTPSEGAQILDQWIPGDT

SVNMTIYATHRDPKVFPDPETYNPMRWMDPNERRRMEPLFIPFSAGARGCIGRNISYLEQIVVLASLVHR

YDFALPSPDFEMQRVEAFNIICGQLPIKIWRRKIE*

 

>CYP630A3 Aspergillus oryzae

AP007164.1a

CDS complement(738572..740134) no introns

First P450 of six on this accession

62% to 630A2

MPGIYVLAAAGALALGVGYVVILPIIHYFYDPKGFRKYPNFAPL

AGITDLPYCYLSSCGYRSKDLYEAHKNAPILRIGPNNLSFGRIGAVKDIYGHNTPCIK

DIKYAMTWGSHTHLFDVIDKADHAAKRKRMSSAFAIKNSERWEHKVANVTGRLVKALD

AHCTLPLLPGQTEPQAADVTLDYNKWINLFTIEAINLIALSSTLGLLEKGSDEVTAQR

KDGTTYPARYRKSQDSTAHAQSLFVWDYKYFHWLSRLSKLVPKYRQMWKDGEPWGDVI

YHQAVTRLQRYQSGEKLDDFFSSLMEDKAGHPNNLEWGEIVAEVGAIINAGADTTAIA

LTQVLDILIRHPKYLQRLREEVDSTLDADEVVAPYDKVKNLPFLRACLDEALRLIPPT

SAGLPRRTPPEGAQILNEWIPGDTSVSMTSYSAHRDPEIFPVPEEYNPDRWMDLDNRK

RMEPYFVPFSTGARGCLGRNITYLEQTVVLATLVHRYDFAVPANWKLGRFEAFNLIMG

EMPMKIWRREKA

 

>CYP630A3 Aspergillus flavus

98% to CYP630A3 Aspergillus oryzae

AFL2G_05913

MTGIYVLAAASALALGVGYVVILPIIYYFYDPKGFRKYPNFAPLAGITDLPYCYLSSCGYRSKDLYEAHKNAPILRIGPN

NLSFGRIGAVKDIYGHNTPCIKDIKYAMTWGSHTHLFDVIDKADHAAKRKRMSSAFAIKNLERWEHKVANVTGRLVKALD

AHCTLPLLPGQTEPQAADVTLDYNKWINLFTIEAINLIALSSTLGLLEKGSDEVTAQRKDGTTYPARYRKSQDSTAHAQS

LFVWDYKYFHWLSRLSKLVPKYRQMWKDGEPWGDVIYHQAVTRLQRYQSGEKLDDFFSSLMEDKAGHPNNLEWGEIVAEV

GAIINAGADTTAIALTQVLDMLIRHPKYLQRLREEVDSTLDADEVVAPYDKVKNLPFLRACLDEALRLIPPTSAGLPRRT

PPEGAQILNEWIPGDTSVSMTSYSAHRDPEIFPDPEEYNPDRWMDLDNRKRMEPYFVPFSTGARGCLGRNITYLEQTVVL

ATLVHRYDFAVPANWKLGRFEAFNLIMGEMPMKIWRREKA*

 

>CYP630A4 Fusarium oxysporum

FOXG_13427, FOXG_13426 Join models

85% to CYP630A1

Supercontig_19 936278-937336 (-) strand

Supercontig 19: 932834-933337 (-) strand

C-terminal half 3000 bp away with no good boundary, possible pseudogene

MIGFILLGAGLAALLIRLFIYPIILYFIDPKGLRKYANFSWLSPLTDLRHCYLSHTGTRSETLAAEHDRRDEHILRIGPN

ALSFSHPQAVRDIYGHQTKCTKDHKETVLAGTHRNLFDVVDKGDHSRKRRLLSAAFATRNLEKWEFKVQYTTQRLLDAFD

QRCTGPLKPNTNPDPEDLTVDFNHWINLWTIEAINYIALSSKMTLLETGTDEVLAEKPDGTLYPARYRQAQNAAAMCKSI

FVWEYELYPWLARISKVVPNKWKSYWATSAPWGDICYHQAKDRLRRYEAGEKLDDFFSCLMVNKNGIPNELEWGEIVAET

SAIIDAGAETTAIALTHILQLLISHPKQMQ (?)

KLRDEVAAVMDEDDIIAPFDKVKDLPYLKACLDEGLRLIPPVSSGLPRRTPPEGAKIMNT

WIPGDTSVSMTIWSAHHNEDIYPEPYVFKPERWLNLEERKRMEPFFIPFSAGARGCIGRN

ISYLEQQIVLATLVHRYDFAMPTKDFKVDRFEAFNLIMGKLPVKIWRR*

 

>CYP630A4 Fusarium verticillioides

96% to CYP630A4 Fusarium oxysporum = ortholog

FVEG_11857

MIGFVLLGAGLAGLFIRLVIYPIILYFIDPKGLRKYANFSWLSPLTDL

RHCYLSHTGTRSETLAAEHDRRGEHILRIGPNALSFSHPQAVRDIYGHQTKC

TKDHKETVLAGTHRNLFDVVDKSDHSRKRRLLSAAFATRNLEKWEFKVQYTT

QRLLDAFDQRCTGPLKPNTNPDPEDLTVDFNHWINLWTIEAINYIALS

SKMTLLETGTDEVIAEKPDGTLYPARYRQAQNAAAMCKSVFVWEYELYPW

LARISKVVPNKWKSYWATSAPWGDICYHQAKDRLRRYEAGEKLDDFFSCL

MVNKNGVPNDLEWGEIVAETSAIIDAGAETTAIALTHILQLLISHPKQMQ

RLRDEVAAVMDEEDVISPFDKVKDLPYLKACLDEGLRLIPPVSSGLPRRT

PPEGAKIMETWIPGDTSVSMTIWSAHHNEDIYPEPYVFKPERWLDLEERK

RMEPFFIPFSAGARGCIGRNISYLEQQIVLATLVHRYEFAMPTKDFTVDR

FEAFNLIMGKLPIKIWRR*

 

>CYP630B1 Fusarium graminearum AACM01000338.1 revised

MYAQLVTASTALCLVTYFILYPIFQYLRDAKGLRRYPNFHPLAGVTNLPF

VREAARGFRSKTLYEMHKTHPVIRTGPNSLSYGSVQAIKDIYGHGTKCTK

GEFYETLAGTHYHLADVVDKADHARKRRALSAAYALKNLENWEFKVADKA

ERFIRAADAACTLPLKRGFARPGPEDLKFDYRAFTNFFTLDAIADIGLSE

RLGFLDQGHDLVKGERMDGSIHHVNFRECLHSTARAQSILAWTEKWYNAN

VKLSKLFSKDFRKWWNLNEGWNDIVYHRATQRLERYNKGEKLPDFFQALM

DHSEANPPGLEWGEIVAEVSIMMNAGSDTTAIAMNNVMYWLLKNPSCMAK

LRQEVDSVLDPEEVVAPYDKVKHLPYLRACLDESLRITPPTTFGLPRRTP

PEGWNIMGDYVPGDTTVSISAYVAHRDPHIFPDPESYVPERWLGEKGKDL

QSYFISFSTGARGCI GRNISYLEQTVLLASVVHRYEWALPYAKWEPSRTE

AMNLAPGPMPLKVWRRDLGGDENDEKHG*

 

>CYP630B2 Aspergillus nidulans AN5837.1 60% to 630B1 53 clan

revised 7/19/07

MITLTYAAITGLLVLVYFVIWPVFEYFRDPKGLRRYPNMSIFSGISAIP

FMLMASRKFRSKELQELHKKHPVLRTGPNSLSYGDVRAIKDIYGHNTRCG

KDPSYIVSAGSHYHLADVIDRADHSRKRKVLSSAYALKNLETWEHKVSDK

IARLVKHFDSVCTAPLPAGQEEPDPADLTVDFRAWTNFFSLDAIVDIGLS

EKLGFLDQGHDMCTAERKDGTTYRASLRDALYPNARKQSLLLWNYDWYPI

INRWVNIIPYFAKMQKSSDTWEDIVWHHAMQRRRRYEAGEKLEDFFSALM

EDKAGHPLGLEWGEVCAEINIMMNAGSVTTAIAITNVMYQLLRNPQCLAT

LREEIDSVYDSEDEVVASYDKVKHLPYLRACLDESLRIFPPTSHGLPRET

PPEGMEILGQWVPGKTSVSMSAYVAHRDETVFPEAYLYKPERWLGEEGKA

LQPYFVAFSAGARSCIGRNISYLEQTVILATLVRR

YEFALPSKDWELQREETMNLILGGMPVKVWRRQLDGDA

 

>CYP630B3P Aspergillus nidulans AN2191.1  55% to 630B1pseudogene

MLEPILALLLVYSFYSYLVAPIITYIWDPKNLRRYPNFHPLPGISDIPFLLAAQKVF

RSRTLHALHQHHPIVCVGPNALSYGAPTAIKDIYGHGT

TCVKDRFYSEKSGSHAHLANVV

GKQDHARKRKVLASAYAIKNPEG*EYKLSGMTARLMRV

FEGKCTEPLPSSV*ATEERDLTIDFRSLTNHFT

VVAIANIGLTEDL

GFHEQGSDTNSLESMDGRVKDVSFKECREASGTVAYRLIWAYDWFPVLKR

LSKVLSPYYRKLGKLDADWNGIVYNRATRRLNV

GEKLDDFFTAMMEDKKGAAHNLETGEIVAEI

SIMMNAGSDTTAIALRNVLFLLLKNPRCMARLREEINVVLDEDELVAPYS

KVKHLPYLRACLDESLRMLPPVVFGLPRRTPPEGTTILGEHVA

GDTSVSMSAYVVHDESFFKDNSTYLPERWLGE

RSKSLQSYFIPFSARARGCI GRNISYL*QTVVLASL VHRYDFALP

XXXXXXXXXXATNLSPGPMPLKFGRGFMHDE*

 

>CYP630B4 Nectria haematococca

e_gw1.1.300.1

Necha1/scaffold_1:1594762-1596339

84% to CYP630B1 no introns

This gene model seems correct

MYAEAVVIFTAASLITYFILYPVIEYLRDAKGLRRYPKFHPLAGISNLPFVREAARGFRSKTLYEMHKEH

PVIRTGPNSLSYGSVQAIKDIYGHGTKCLKGEFYETLAGSHFHLADVVDKADHARKRRALSAAYALKNLE

NWEFKVADKTERFIRAADAACTLPLKKGFVRPDPTDLTFDYRAFTNFFTLDAIADIGLSERLGFLDQGHD

LVKAERMDGTIHEVNYRECLHSTARAQSILAWTEGWYNFNVELSKWISVDFRKWWHLNEGWNDIVYHRAT

QRLERYKKGERLPDFFQALMDHSDANPPGLEWGEIVAEVSIMMNAGSDTTAIAMNNVMYWLLKNPSCMAK

LREEVDSVLEPDEIVAPYDKVKHLPYLRACLDESLRITPPTTFGLPRRTPPEGWNILGDFIPSDTTVSIS

AYVTHRDPSIFPEPESYVPERWLGEQGKDLQPYFIAFSAGARGCIGRNISYLEQTVLLASVVHR

YEWALPYPEWEPERREAMNLSPGPMPLKVWRRELEVEEKQG*

 

>CYP630B5 Nectria haematococca

e_gw1.52.31.1

Necha1/scaffold_52:135577-137148

65% to 630B1, no introns

This gene model seems correct

MYAYLLLAAVAVFFVFTFVLHPLYAYLSDPKHLRRYPNFHPLSGITNIPFMVEATRGFRSKALREQHKKR

PVIRIGPNSLSYGTGRAIKDIYGHGSGCIKGELYEVLAGTHFHITDVIDKYEHQRKRKAVSAAFALKNME

NWEYKVADKTERFIRACDKACTPPLKKEMTRPNPEDLTFDYRSYTNFFSIDAIADIALSEHLHLLDKGTD

IVQAERMDGSKFQVNFRDCLHGTARAQSIIVWASQWFKFNKWFTRLVSPEFRRLWKLNDGWEALVYNRAT

ERLKRYQNGEKLSDIFQAFMEDRDGNPRGLEWGEIVAEVSILMNAGSDTTGIAMCNVMYWLLKHPDCMTR

LREEVDAVLDADEVVAPYDKVKHLPYLRACLDETLRISPSTSFGLPRRTPAEGAYILGDFIPGDTTVAIS

AYVAHRDEEIFPDPDKFIPDRWLGEKGKDLQPFFIAFSAGARGCIGRNISYLEQTVLLASVVHR

YEFALPHPDWEQTWLEALNAMPGKMPLKVWRRDLSNREV*

 

>CYP630B6 Nectria haematococca

e_gw1.20.309.1

Necha1/scaffold_20:354486-356086

60% to 630B1 F. gram., 58% to e_gw1.52.31.1

This gene model seems correct

MYAVVVSVSFLSFLVYYFVLSPVWLYLRDPKGFRKYPAMNSLAGITDLGFMYEASKGFRTKRLTELHKKY

PVVRIGPNSLSFSGVQAIKASP (0)

EIYGHGTPCTKDHFYVVTGGSHTHLADVVNKADHARKRRVLSSAYAIK

NLEDWEYKVADKTERFIKACDAACTLPSKGSADPHPDELTFDYRKWTNFFTVEAIADIGLSEKLGFLDNG

SADCTAEKRDGTLYTANFVESLHNTAIAQSRLVWGYSWYKTNEYLARLLSKDFRRMLRLGDQFDDIVYHR

ATKRLARYRAGEKLDDFFQALMEDKNGRQNNQEWGEIVAEVSIMMNAGSDTTAIAMNNAMYHLLKHPDVL

KKLQEEVDSALDEEEEVAPFAKVKHLPYLRAVIDETLRITPSVTFNLPRRTPQGGCAIGDEFIPGETSVS

ISAWTAHRDEEVFPEPEEFRPERWLGEGAQELQRGFIAFSTGARGCIGRNISYLEQTVLLASVVHRYGMA

LPSKDWTPELDEGTNLLTKEMPVKVWRRRV*

 

>CYP630B7 Nectria haematococca

fgenesh1_pg.scaffold_50000029

Necha1/scaffold_50:99287-100920

58% to 630B1, 60% to e_gw1.1.300.1

This gene model seems correct

MSVSVASLGTTPYAAALGVVAVIYFLIYPFVLYLRDSK (1)

GLRRFPNMSTFSGMSNLPFMILAHGGARSSAM

AKMHKTEPILRTGPNTLSFGSVKAIKGIYGHGTPCIKDESYVLTAGTHFHLADVIDKPDHARKRKVLSSA

YALKNLEEWEYKVADKVQRMMNHFEKCCTAPLSKGVKYPNPEDVNVDFRAWSNFFSLDAIADIGLSEKLG

LLDRGHDRVEAQEKNGTIFEANLRECLYPTARKQSLLLWSYDNYKLLDKISNIIPYYRRMSESSRGWDAI

VLRRANLRLQRYNAGEKLDDFFQALMEDKSGNPHNLEWGEVVAEINIMMNAGSVTTAIALANVLYQLLLN

PDKMETLRNEIDSVLEPDEMVAPYDKVKHLPYLRACLDESLRLWPPTPQSLARKTPPEGTTIMGQYIPGN

TTVGISALVAHRDETAYPEAEKYLPERFLGEKGKELQSSFITFSAGARGCIGRNISYLEQAVCLASIVHR

YDFALPAGFELKREETMNHILGEMPVKVWRRDLGEEE*

 

>CYP630B8P pseudogene Nectria haematococca

fgenesh1_pg.scaffold_30000003

Necha1/scaffold_30:23907-24329

55% to fgenesh1_pg.scaffold_50000029 mid to heme, 52% to 630B1

add cyan region (seq missing after GLNVI)

RLERNRRMEKLDDTFQALTRHQTGTPHNLEWGQVVVEVNIMTNAGSVATAVALTNVFYRVIRTP

RARALLRKEPDAALEPDKVIALDDKAKHPPYLRACLHECPRLFPPTTHGLPRKTSPDGLNVI

LGKKSKELQPWFITFSTGSRACIGRNISYPEQTVLIA

 

>CYP630B9  Aspergillus fumigatus

          GenEMBL XP_755127.1 EAL93089

join(AAHF01000001.1:159023..159128,

                     AAHF01000001.1:159173..160644)

          61% to CYP630B1 FG

MWSLFTIAPYACILGLLLFVYFIAYPFVEYIRDPKGLRRYPNFHPFSGMSAIPFMVLASRGFRSMELLNL

HRKHPVLRTGPNTLSYGDVRAIKDIYGHNTKCIKDPSYIVTAGTHYHLADVVDKPDHARKRKVLSSAYAL

KNLETWEYKVSDKLERLIAHFDRVCTKPPSAAVAEGKAAPDPADLTVDFRAWTNFFTLDAIADIGLSEKL

GFLDSGSDVCTAERKDGTTYEVHLREALYPTARKQSLILWNYEWYPVLNKLVNIIPFFRRMQRSSDNWEN

IVWRRASQRLRRYEAGEKLDDFFQALMEDKNGHPNNLEWGEIVAEVNIMMNAGSVTTAIAIANVMYQLLK

NPRCLAKLREEVDAVLDEEDIIAPYDKVKHLPYLRACLDESLRIFPPTSHGLPRQTPPEGMEILGQWVPG

NTSVSISALVAHRDESVFPQADQYIPERWLGEEGKALQPYFVAFSAGARSCIGRNISYLEQTKAIATLVH

RYEFALPHPGWELKRLETMNLILGDMPVKVWRRQT

 

>CYP630B9 Neosartorya fischeri

97% to CYP630B9  Aspergillus fumigatus = ortholog

NFIA_083610

MWSLFTIAPYACILGLLLFVYFIAYPFAEYIRDPKGLRRYPNFHPFSGMSAIPFMVLASRGFRSMELMKLHRKHPVLRTG

PNSLSYGDVRAIKDIYGHNTKCIKDPSYIVTAGTHYHLADVVDKPDHARKRKVLSSAYALKNLETWEYKVSDKLERLIAH

FDRVCTKPPSPAVAEGKAAPDPADLTVDFRAWTNYFTLDAIADIGLSEKLGFLDSGSDVCTAERKDGTTYEVHLREALYP

TARKQSLILWNYEWYPVLNKLVNIIPFFGRMQRSSDNWDNIVWRRASQRLRRYEAGEKLDDFFQALMEDKNGHPNNLEWG

EIVAEVNIMMNAGSVTTAIAIANVMYQLLKNPCCLVKLREEVDAVLDDDEIIAPYDKVKHLPYLRACLDESLRIFPPTSH

GLPRQTPPEGMEILGQWVPGNTSVSISALVAHRDESVFPQADRYIPERWLGEEGKALQPYFVAFSAGARSCIGRNISYLE

QTKAIASLVHRYEFALPHPGWELKRLETMNLILGDMPVKVWRRQRDMI*

 

>CYP630B10  Aspergillus fumigatus Af293

           GenEMBL XP_748668.1 also EAL86630.1

           46% to 630B2 with errors

           closer to 54% if gaps removed, needs revision

some parts of the middle of this sequence may be out of order

MLGMMLLLGLLARLFFRFVIWAIFVYLRDPKGLRKYPLMHPLSGISDIPFMLESMRGFRSATLLQLHYGQ

GHPVIRLGPNALSFAGGQAIPAIYGHNTPATKDRQYLNAAGSHFHLADVVDKKEHARKRKVLASAFAAKH

LEDWEYKVADKVQRLMQRFDQHLAQMPDDPLDYRSWTNLFTIDSLCDTLIVAPTVSFRDCLYATFHIVGD

LVWSYEWYHVVSWLLQRVSAHDIFAALMEDPAGNPQDLEWGEVLAEISLAISGSSSTSNSIASTMQLLIE

HPEKMRKLQEEVDSVMATQLEDSPDGDTPTVASYDQIKSLPYLRAVIDESLRLYPPISHGLPRETPKEGM

MIMDQWVPGNTTVSVSAYVAHRDPAVFDQPESFVPERWLGEQGRALQTRFIAFSAGARGCIGRPISYLQA

SILLANLVHQYDFEMWDPKWKPSRRETMNLIMGPMPPGGFSGEGG

 

>CYP630B11  Aspergillus oryzae

           GenEMBL BAE66547.1

           59% to 630B2

MYSLLLIVTLHFLLIYYIIIPIINYFRDPKGLRKYPNLTFVSGISDLPLIYYSHKGIRSRVLFEAHKKHP

VLRIGPNKLSYADPVAIKDIYGHGTKCTKDVFYSALSGSHYHLADVVDKEDHARKRKVLSNAYAIKNLEG

WEYKVADMTQRIIKAFDERCTSPLPKGRNPDPQDLTIDYRMWTNLYTIAAIANIGLSEDIRFLDQGNDII

SSEAKDGTVKKVHFRDCLYANASATSTLVWAYDWYEALVRVSKLVSSTYRQKWKLAEDWDGIVNNRATTR

WKRYEKGEKLDDFFSALMEDKAGAPNNLEWGEIVAEVSIMMNAGSDTTGISLNNVMLLLLKNPHCLEKLR

EEIDGVLEDDEVIAPYDKVKHLPYLRACLDENMRMYPPVSFHVPRRTPQEGTMIRGEFVAGNTSVGISAY

VVHRNEDIFPDPDTYKPERWLGDKGRDLQPYFVAFSAGARGCIGRNISYLEQTVLLASLVHRFEFALPSP

SWEPVRHETTNFNSGPMPLKVWRRASRVYEDN

 

>CYP630B11  Aspergillus flavus

100% to CYP630B11  Aspergillus oryzae

AFL2G_11866

MYSLLLIVTLHFLLIYYIIIPIINYFRDPKGLRKYPNLTFVSGISDLPLIYYSHKGIRSRVLFEAHKKHP

VLRIGPNKLSYADPVAIKDIYGHGTKCTKDVFYSALSGSHYHLADVVDKEDHARKRKVLSNAYAIKNLEG

WEYKVADMTQRIIKAFDERCTSPLPKGRNPDPQDLTIDYRMWTNLYTIAAIANIGLSEDIRFLDQGNDII

SSEAKDGTVKKVHFRDCLYANASATSTLVWAYDWYEALVRVSKLVSSTYRQKWKLAEDWDGIVNNRATTR

WKRYEKGEKLDDFFSALMEDKAGAPNNLEWGEIVAEVSIMMNAGSDTTGISLNNVMLLLLKNPHCLEKLR

EEIDGVLEDDEVIAPYDKVKHLPYLRACLDENMRMYPPVSFHVPRRTPQEGTMIRGEFVAGNTSVGISAY

VVHRNEDIFPDPDTYKPERWLGDKGRDLQPYFVAFSAGARGCIGRNISYLEQTVLLASLVHRFEFALPSP

SWEPVRHETTNFNSGPMPLKVWRRASRVYEDN

 

>CYP630B12  Aspergillus oryzae

           GenEMBL BAE65338.1, AP007171.1

           57% to 630B11, 75% TO 630B13, 77% to 630B2

          14 P450 genes and 2 pseudogenes on this contig

MWSALSIAPYALVLGLFSLLYFVVFPWVEYIRDPKGLRKYPNMNPFSGMSAVPFMLLASRGFRSKELQEL

HRTKPVIRTGPNMLSYGDVRAIKDIYGHNTKCIKDPSYIVTAGTHYHLADVVDKPDHARKRKVLSSAYAL

KNLETWEHKVSDKVEKVVAHFDKVCTAPPSAAVAAGKMAPDPKDLTVDFRAWTNFFTLDAIADIGLSEKL

GFLDSGSDVCIAERKDGSTYEVNLREALYPTARKQSLILWNYEWYPVLNKMVNIIPFFNRMQNSSDNWDN

IVWRRSMNRLRRYEAGEKLEDFFQAMMEDKNGRANNLEWGEIVAEMNIMMNAGSVTTAIAIANVMYQLLR

NPQSLKKLQEEIDAVLDADEIVAPYDKVKHLPYLRACLDESLRIFPPTSHGLPRETPPEGMEILGEWVPG

NTSVSMSAYVAHRDESVFPKADQYIPERWLGEEGKALQPYLIAFSAGARSCIGRNISYLEQTKILATLVH

RYDFALPYPGWELKRLETMNLILGDMPVKVWRRNVQEA

 

>CYP630B12  Aspergillus flavus

100% to CYP630B12  Aspergillus oryzae

AFL2G_05615

MWSALSIAPYALVLGLFSLLYFVVFPWVEYIRDPKGLRKYPNMNPFSGMSAVPFMLLASRGFRSKELQELHRTKPVIRTG

PNMLSYGDVRAIKDIYGHNTKCIKDPSYIVTAGTHYHLADVVDKPDHARKRKVLSSAYALKNLETWEHKVSDKVEKVVAH

FDKVCTAPPSAAVAAGKMAPDPKDLTVDFRAWTNFFTLDAIADIGLSEKLGFLDSGSDVCIAERKDGSTYEVNLREALYP

TARKQSLILWNYEWYPVLNKMVNIIPFFNRMQNSSDNWDNIVWRRSMNRLRRYEAGEKLEDFFQAMMEDKNGRANNLEWG

EIVAEMNIMMNAGSVTTAIAIANVMYQLLRNPQSLKKLQEEIDAVLDADEIVAPYDKVKHLPYLRACLDESLRIFPPTSH

GLPRETPPEGMEILGEWVPGNTSVSMSAYVAHRDESVFPKADQYIPERWLGEEGKALQPYLIAFSAGARSCIGRNISYLE

QTKILATLVHRYDFALPYPGWELKRLETMNLILGDMPVKVWRRNVQEA

 

>CYP630B13  Aspergillus oryzae

           BAE63103.1

           75% TO 630B12, 62% to 630B7

MLSLVLVAPYAAVGGLCTLLYFFVFPFIEYIRDPKGLRKYPNLHPISGMSVLPFMFMASRGARSQELSEL

HKKSPVIRTGPNTLSYGDVRAIKDIYGHNTKASKDPSYIVSAGTHYHLADVVDRADHARKRKVLSSAYAL

KNLETWEYKVSDKIERLVKHLDKCCTAAPVSGGRAFVPSSEDLTVDIRAWINFFTLDAMADIGLSEKLGF

LDKGNDICVAERKDGSTFECGLRDALYPLAIKQCMVLWNYEWFPIINKLVDVFPYFRNLQKKGDAWEHII

WRRSSERLRRYEAGEKLNDFFQALMEDKNGRPNNLEFGEIAAEVNIMMNAGTVTTAIAITNVLYQLIRHP

EAMAKLREEIDGVLGPDEIVASYDTVKHLPYLRACLDESLRILPPTPHGLPRQTPPEGMEILGEWVPGNT

LVSISAYVAHHDESVFPQPHKFIPERFLGEAGKELGPYFITFSAGARSCIGRNISYLEQTKALATLVHRY

DFALPYPDWEPKRFESMNHILGEMPIKIWRRSFDG

 

>CYP630B13  Aspergillus flavus

99% to CYP630B13  Aspergillus oryzae

AFL2G_08530

MLSLVLAAPYAAVGGLCTLLYFFVFPFIEYIRDPKGLRKYPNLHPISGMSVLPFMFMASRGARSQELSELHKKSPVIRTG

PNTLSYGDVRAIKDIYGHNTKASKDPSYIVSAGTHYHLADVVDRADHARKRKVLSSAYALKNLETWEYKVSDKIERLVKH

LDKCCTAAPVSGGRAFVPSAEDLTVDIRAWINFFTLDAMADIGLSEKLGFLDKGNDICVAERKDGSTFECGLRDALYPLA

IKQCMVLWNYEWFPIINKLVDVFPYFRNLQKKGDAWEHIIWRRSSERLRRYEAGEKLNDFFQALMEDKNGRPNNLEFGEI

AAEVNIMMNAGTVTTAIAITNVLYQLIRHPEAMAKLREEIDGVLGPDEIVASYDTVKHLPYLRACLDESLRILPPTPHGL

PRQTPPEGMEILGEWVPGNTSVSISAYVAHHDESVFPQPHKFIPERFLGEAGKELGPYFITFSAGARSCIGRNISYLEQT

KALATLVHRYDFALPYPDWEPKRFESMNHILGEMPIKIWRRSFDG*

 

>CYP630B14 Aspergillus niger

fgenesh1_pm.C_scaffold_20000049|Aspni1

77% to CYP630B11

MYIPIVILLLSLLTIYYFIIPIINYFRDPKGFQKYPNLTFWSGISDLPLVYFSHKGIRYKRLLEAHKTHPVVRIGPNYLS

YADPDAIKDIYGHGTTCTKDLFYSTLGGSHFHLADVVDKEEHARKRKVLSSAYALKNLETWEYKVADMTNRLIKAFDARC

TDPLPKGHRPLEKDLTIDYRMWTNLFTIAAIANIGLSEDIKFLDQGNDLITSESMDGMTKKVHFRDCLFATGSATATLVW

SYDWYKTLARASKILSPTYHRKWKLNKDWDGIVYNRATTRWKRYQNGEQLDDFFSALMADKSDVPHNLEWGEIVAEISIM

MNAGSDTTGISLSNVMLLLLNNPACLEKLQEEVDGVLDDDEIVAPYDKVKHLPYLRACLDESLRLYPPVSFALPRRTPPE

GTMILSDHIAGNTSVGMSAYVVHRNESIFPDPEAFRPERWLGEKGKELQPYFIPFSTGARGCIGRNISYLEQTILVASLV

HRFDFALPYPGWEPERHETTNLGSGPMPMKVWRREVAA*

 

>CYP630B15 Aspergillus niger

fgenesh1_pm.C_scaffold_9000258|Aspni1

88% to CYP630B12

MWSLFTIAPYAFFIGIVGISYFLVWPFVQYIRDPKGLRKYPNLTPISGMSAIPFMLMASRGFRSRELQELHQEKPVIRTG

PNTLSYGDVRAIKDIYGHNTKCIKDPSYVVTAGTHYHLADVVDKGDHARKRKVLSSAYALKNLETWEHKVSDKVEKVVAH

FDKVCTAAPSAAVASGKVAPDPKDLTVDFRAWTNYFTLDAIADIGLSEKLGFLDQGSDVCIAERKDGSTYTVHLREALYP

TARKQSLILWNYEWYPVLNKLVNVIPFFGRMQKSSDNWDNIVWRRSIERLRRYEAGEKLDDFFQALMEDKNGRANNLEWG

DVVSEVNIMMNAGSVTTAIAIANVMYQLLRNPECLAKLREEIDAVLDADEVVAPYDKVKHLPYLRACLDESLRIFPPTSH

GLPRETPEEGMEILGQWVPGKTTVSMSAYVAHRDERAFPKADQYIPERWLGEEGKALQPYFVAFSAGARSCIGRNISYLE

QTKILATLVHRYEFALPYPGWELKRLETMNLILGDMPVKVWRRQVPATD*

 

>CYP630B16 Fusarium oxysporum

90% to CYP630B4 Nectria haematococca

FOXG_03207

MYAQVVSVFTAVCLLTYFALYPIYTYLRDAKGLRRYPNFHPLAGITNIPFVREAAKGFRSRTLYEMHKTHPVIRTGPNSL

SYGSIQAIKDIYGHGTKCTKGEFYETLAGSHYHLADVIDKADHARKRRALSAAYALKNLENWEFKVADKAERFIRAADAA

CTLPLKEGFTRPDPRDLNFDYRAFTNFFTLDAIADIGLSERLGFLDQGHDRVKAERMDGTIHEVNFRECLHSTARAQSIL

AWTEKWYNFNVEVSKWFSKDFRQWWHLNEGWNDIVYHRATQRLERYKNGERLPDFFQALMDHSEANPPGLEWGEIVAEVS

IMMNAGSDTTAIAMNNVMYWLLKNPSCMAKLREEVDSVLDPEEIVAPYDKVKHLPYLRACLDESLRITPPTTFGLPRRTP

PEGWNILGDYIPGDTTVSISAYVTHRDPQVFPDPESYAPERWLGEKGKDLQPYFIAFSAGARGCIGRNISYLEQTVLLAS

VVHRYEWALPYPEWEPERREAMNLAPGPMPLKVWRRDLGVEEVDEKGN*

 

>CYP630B17 Fusarium oxysporum

81% to CYP630B7   Nectria haematococca

FOXG_02681

MSASVASLGSAPYAAAVGFAVVFYFIIYPFILYLKDPKGLRRFPNMSPFSGMSNLPFMILAHGGARSTHLAKLHKTKPII

RTGPNSLSFAGGQAIKDIYGHGTPCTKDKSYIVGAGTHFNLADVVDKHDHARKRKVLSSAYALKNLEGWEYKVADKVQRM

MDHFDKVCTAPLQKGARFPDPQDVNVDFRAWSNFFSLDAIADIGLSEKLGLLDQGHDRVEAQQTCGTVYETNLRECLYPT

ARKQSYLLWTYDYYKLLNKISNVIPFYRKMSESAKGWDDIVLRRAQIRLDRYQKGEKLDDFFQALMEDKNGNSHELEWGE

IVAEINIMMNAGSVTTAIALANILYQLILNPRVMELLRHELDSVLEPDEVVAPYEKVKHLPYLRACLDESLRLWPPTPQN

LGRQTPPEGLTIMGQYIPGNTSVGVSALVAHRDECIYPESEKFIPERFLGEKGKELQSHFITFSAGARGCIGRNISYLEQ

TVCLASIVHRYEFALPAGFELKREETMNHILGPMPVKVWKRNLEE*

 

>CYP630B17 Fusarium verticillioides

95% to CYP630B17 Fusarium oxysporum

FVEG_01518

MSASIASLGSAPYAAAVGVAVIIYFIVYPFIAYLKDPKGLRRFPNMSTFSGMSN

LPFMILAHGGARSTHLAKLHKTKPIIRTGPNSLSFAGGQAIKDIYG

HGTPCIKDESYIVGAGTHFHLADVVDKHEHARKRKVLSSAYALKNLEGWEY

KVADKVQRMMNHFDKVCTAPLPKGAKFPDPQDVNLDFRAWSNFFSLDAI

ADIGLSEKLGLLDQGHDRVEAQQTCGTVYKANLRECLYPTARKQSYLLWTYDH

YKLLNKISNIIPFYRKMSNSAKGWDDIVLRRAQIRLDRYQRGEKLDD

FFQALMEDKNGNSHELEWGEIVAEINIMMNAGSVTTAIALANILYQLILNP

RVMELLRQELDSVLEPDEAVAPYEKVKHLPYLRACLDESLRLWPPTPQN

LGRQTPPEGLTIMGQYVPGNTSVGVSALVAHRDESIYPESEKFIPERFLGE

KGKELQSHFITFSAGARGCIGRNISYLEQAVCLASIVHRYEFALPAGFE

LKREETMNHILGPMPVKVWKRNLEE*

 

>CYP630C1 Nectria haematococca

e_gw1.19.274.1

Necha1/scaffold_19:134969-136557

46% to 630B1, 630B1 has a deletion near heme signature

same gene as fgenesh1_pg.scaffold_19000054

This gene model seems correct

MESFIVFTTALFLLFIIEPFARYLWDPQNLRRFPSHNPLSSITNLGYIIERCRGFRSRNLHEAHKKHSVV

RIGPNSLSFSSPKAIRSIYGHSTNCIKGDMYAASAGPHDSLLDVIDKEAHARKRRYMSHALATRNLETWE

HKVADKVRTLVRQFDRICDGAASATNTKTGTLDFRKWTNLFTIEAIADIALSHKLGCLDRGDDQVTVTTA

DGNEKRVHYIKCLHAGKRATSTLVWANSWFRFLRTVLTLLPGWFRSQWLKGAQFDDMVHHLTRVRVERHQ

TGETLDDIVHCLIEDKNGELRGLDIGEIEAEVGVL (1)

LDAGSDTTAIALTHVMHSLLRHPNALQRLRDELKE

TLDVDNPVASYDQVKNLPYLRACLDESLRLLPPVSFGLNRKTGPGGLTIDGQWIDEGITVGVPAYTAHRN

PQLFPDPERYHPERWLEEMHKDAKASFIPFSAGARGCIGRNITYMEQMILIASLVQRYDFALPDDGWELD

HEEAFNLWPGPMPLSISKR*

 

>CYP630D1  Aspergillus oryzae

          GenEMBL BAE60571.1

          43% to 630B2, 48% to 630C1, 44% to 630B5, 43% to 630A3

MALEKLFIVVFLCFALRPLLLYFYDSKKLRKYPNQNFLSGVTNLASIRERQRKFRTRELYLQHQKHPIIR

VAPNMLSFRDVKAIKDIYGFGSPCQKHEIYKLQNSEGHMNILNVIDREDHNRKRRMLSHAFSTKNLESWE

FKITDKVEKLVAQFDRRAHPPAWKNEPSQQNNTTVDVRYWLNLFTVDAIADIALSERLGMLESGSDVVKV

GGPGEEDSAHRFIEDMHEAARVKSKIIGTLDWYYVLKQVSSFLSSRCRSQWDCGGNVGQIVEHLAGKRLR

RHEDGENIDDFLSCLINDKAGKSRNLDIGELKAETSILLDAGSETTAIALTHLLYYLIKNPDCFVKLRKE

VSGAIAGDKVAPYAKVKSLPYLKACIEESLRLSPPLPRGLERVTPAAGAYIMGEFIPGNVGVSVPAYVAH

RDPDLFPEPEAFLPERWFNNENIGKMRDAFIPFSAGGRACIGRNITMIEQQILIATLVHRYDFSLASPDW

TLQNEEAFNLWPVELPVKIWERDLEA

 

>CYP630D1  Aspergillus flavus

98% to CYP630D1  Aspergillus oryzae

AFL2G_03294

MALEKLFIVVFLCFALRPLLLYFYDSKKLRKYPNQNFLSGVTNLASIRERQRKFRTRELYLQHQKHPIIRVAPNMLSFRD

VKAIKDIYGFGSPCQKHEMYKLQNGEGHMNILNVIDREDHNRKRRMLSHAFSTKNLESWEFKITDKVEKLVAQFDRRAHP

PAWKNEPSQPNNITVDVRYWFNLFTVDAIADIALSERLGMLESGSDVVKVGGPGEEDSAHRFIEDVHEAARVKSKIIGTL

DWYYVLKQVSSFLSSRCRSQWDCGGNVGQIVEHLAGKRLRRHEDGENIDDFLSCLINDKAGKPRNLAIGELKAETSILLD

AGSETTAIALTHLLYYLIKNPDCFVKLRKEVSGAIAGDKVARYAKVKSLPYLKACIEESLRLSPPLPRGLERVTPAAGAY

IMGEFIPGNVGVSVPAYVAHRDPDLFPEPEAFLPERWFNNENIGKMRDAFIPFSAGGRACIGRNITMIEQQILVATLVHR

YDFSLASPDWTLQNEEAFNLWPVELPVKIWERDLEA*

 

>CYP630D2P  Aspergillus oryzae

           GenEMBL BAE62696.1, AP007166.1

           Downstream from CYP551A2 (BAE62699.1) (145541-147583) 

           note: two other genes between these

           This gene is a pseudogene missing the top half

           39% TO 630D1, 37% to 630B1

136724  VQTTEYYRSLQEHGKDGCFDNTIFDSVLRSFAEIQEGSNLTKVQTEKLVDEAFAI

MFAGSDTTAFSLTITLVYLCKYPEKIVKLRREVQCLRDFKVQDIQLATISQMPYLDAVIREANRLSSPLS

TVLPREVPSTGCVISGHFLPKGTVVGFHLDDINRNPKFFPEPNDFIPERWSGEEGKKLQRWFVPFSKGSR

RCIGMDFAFVEMKLAVAAIISRFEIWLDNPNVTLNSREMFVKIPEDDLRIRLRAITV

135844

 

>CYP630D2P  Aspergillus flavus NRRL3357

           GenEMBL AAIH01000541.1 pseudogene

           same gene as Aspergillus oryzae BAE62696.1,

           97% identical

           runs off the end

           AFL2G_08101

418 VQTTEYSRSLQEHGKDGCFDSTIFDSVLRSFAEIQEGSNLKVQTENLVDEAFAI

MFAGSDTTALSLTITLVYLCKYPEKMVKLRREVQCLRDFKVQDIQLATISQMPYL (0)

DAVIREANRLSSPLSTVLPREVPSTGCVISGHFLPKG (0)

TVVGFHLDDINRNPKFFPEPNDFIPERWSGEEGKKLQRWFVPFSKGSRRCIGMDFAF

VEMKLAVAAIISRFEIWLDNPNVTLNSREMFVKIPEDDLRIRLRAITV* 1301

 

>CYP630D3 Aspergillus niger

fgenesh1_pg.C_scaffold_2000776|Aspni1

54% to CYP630D1

MALLLTLTLIILALLIKALLTYLLNTKTITLTKYPPLNPLSNLTTLSYTYEHRHPFRTQRLYNLHRQHNHPIIRLSPTAL

SIADPKAIPAIYGHGTPCLKDDVYALTKGTHTHSNMLNTISRDDHSRKRRMLANAFSTKNLENWEFKIVDKVERLLRQLD

GRCSSSSNGEMDGNEVDLRFWLNLFTIDAIADIGLSVQLGLLDRGSEEVDVDGEVVRYIECLHRGNRVSSWVIGASGWVG

FLRGVSRVVSPYLREQWECGRRFGRVVRSLVEQRVSVQEGRETLSDFLGCLNEDKRGVGRELEKGEIEAEAGLLLDAGSD

TTAIALTNVMYYLIKNPQSLIKLREEVSGAILENGVAPYVKVRNLPYLKACLEESLRLSPPVPRGLERKTPPEGMYILGE

RIPGNFTVSVPTYAVHRDPEIFPDPEAFRPERWLEDDEKIKQMRAVFIPFSSGARACIGRNITFMEQQILVATLVHHYEF

ALPSKDWMLEWEEAFNLWPGRMPVRIWRRDE*

 

>CYP630D4 Neosartorya fischeri

57% to CYP630D1

NFIA_005950

Note: this seq does not have an ortholog in A. fumigatus

MAFTLILMVALAYLCARPFIVYFYDAKGLRKYPNLNPLSGITSLAYVWEHSNNFRTRRLYLKHRKHPVIRVGPNVLSFAD

VRAIKDIYGHGTPCRKDDVYSLTVGSHAHILNTVDREDHNRKRRMLSHAFATRNLERWEFKIVDKIGKLITQLDRRCTAP

LSDRHHVNSEDLTLDFTLWSNLFTIDAIADLALSEQLNLLDSGTDVVKVDNIPSLKFIDSLHGGNYITSLFVGAANWFAV

LKALSRIVSPLFRAQWDHGRNYGRIVSALTGKRLEKHQRGGQLGDLFACLMEDNKGNARGLERGELEAEVNVLLDAGSDT

TAIALTHALYYLIKTPHALATLRKEVAGAFSEEPIARYASVKNLPYLKACLEESLRLSPPVSRGLERRTPPEGMRIMGEE

IPGDVGVSVPVYVAHRDPAIFPEPALYRPERWLESEENVKQMREAFVPFSTGARGCIGRNITMIEQQLVIATLVHRYDFA

LPSDHWSLQHNEAFLLWPGPMPLKIWRRDLGSATSMRNDL*

 

>CYP630E1 Uncinocarpus reesii

51% to CYP630B14 A. niger, added back part of heme region

UREG_00268.1

MIGTLIAPICFAALAIIIFKPVALYFLDRKNLRKFPTLHPLSGLSDIPFMVESMKGFRSARLAELHHGKGWPVIRIGPNS

LSFGTVDSIKAIYGHNSPTTKDKQYVNASGTHFHLADVVDKKEHARKRKVLASAFAAKNLEDWEYKVGEKVSRLIDQFDK

YVEESAEGPLNYRSWTNLYTIDCL

VDICLSTQLHCIERGEDTVTAEDLAGITE

DVSFRDCLYATFHLVGDLVWSYDWFST

IITVANTISPVYSRLIKLGTGWRNLVHHLTNKRWSRYAAGERINDIFSSLMDDTAGNAHNLEWGEVVAEISLAVSGSSST

SNTIASTMQLLIENPQKLEKLQEELDSVMEPEEIIASYDKVKYLPYLRAVIDETLRLYPPISHGLPRETPKNGLQILDNW

IAGNTTVSVSAYVAHRDPNAFPEPEKFMPERWLGDEGKQLQPNFIAFSAGARGCI

GRPISYLLAT

VLLASLAHRYDFFKFSSDWKPRRRE

TMNLILGSLPIRIQKRSL

 

>CYP631A1 Fusarium graminearum FG10329.1 AACM01000425 FGcontig1.425_scaffold7

YVSSDFALAFLAIGFSSVLIYQLAHNYRRLQHIPGPLVAKLTDIHRFMLV

RSGFIHLYQASAHERYGSVVRFGPNLVSICDPEAIQEVFNMRNGFNKSNM

YRAFRPWTPAGLLVSVFTAKDDIVNRQMKQHIAVYFSLSYTAASFEERVD

NAIRIFFKQLDSQFVTTGAKIDLTRWFKFFSYDAMGLMTFSRPYGCLENS

SDAAGIISDVKNSMLAIGPMTQMPWLDWLLHKNGLINMIKPEPVSALLKY

VLARISERRNSPKAMHSTVTDGPDANGDFLGYFLQAQEKKSNKVPPQFLS

TWTLANILGGSDSTASMLRSVVCFLVENPDALETMRAELRDKKQHAEGSS

APIPKWTKIQDLPFLNACVIESLRLDPPFATTLERVVPPEGVTICGAFYP

GGTVVGMNPYITNRHRPTWGEDADQWRPSRWLEGDPAHIRKLQASLLSFG

AGTRGCLGQHVAVFEIKKLVVALFMNYNLNLVRLLSQFSKGLS*

 

>CYP631B1 Aspergillus nidulans AN3225.1 38% to 631A1 53 clan

revised 7/19/07

MDHPHPSTFSL

GLSQILVCLALLYAAIHILSV

YRRLCHISGPFWARIS

NLPRVWWVNTSRAHEIHQQLHEKYGDVVRFGPNMVSLRNPTWIPTVYPTR

MGVKKSDFYRTLAPYTPSGALPAVFSSRDEEVHRGLRGPIASLYSMSKVL

PLEVFVDRTIDVLVRQLDGRFAGAGETFDLASWLQFFAFDVMGTLTFSKR

YGFLEKGMDVHGMLDTIWRFLKGAAPFTQIPWVDEIWNKNVLATKLKGAT

GVSILGIVGKFVSQRQEESKAGKIDGTADRDMLSLFMEIQKNNQLPPWYV

TAWTFSNITAGSDSAAVVMRTVFYNLLSHPSTLQKLRSELLSAGPLTQPY

PSWKDVCNLPYLDACILEALRLHPPFCLPFERIVPQGGMVLGDTYFPEGT

VVGMSPWVVNRHKPTFGEDSDVWNPERWMVSKELKSKREAAVLTFGAGRR

VCLGRHIAILELKKIVPALVLRYDFELIDPERFTTENFWFFRQRGMDVRV

KKRMQAEAGI*

 

>CYP631B2  Aspergillus oryzae

          GenEMBL BAE65443.1

          52% to 631B1, 43% to 631A1

          revised 3/19/2009

MLILLGLLCLYTGLYVARTYWRLRHFPGPL

VARFTDLGRLWWVKTSRSHHHHMGLHSRYGQYVRLGPNMISISDPDAIPLVYPIRPGVPK ()

SDFYRSM MPYTRKGRSLPLVFNTRDEDLHKRLKTPIAHLYSLSNILTFEAFVDQVLEILFRQFEERFVPDQAPFNLGNWL

QYFAFDVMGTMSFSRRYGFLEKGRDDTGLLSAIWAFMKAAAPVTQMPWVDLVWNKNPFIALFRATPAQPI

LNVVLSRINDRRNELYSTTSTPEKVNERDFLSRFMHIQSNSDTIPPWAVTAWSFSNVIAGSDTTAVAMKT

LWYNLLLHPATMHRLRKELVQAQQQSKLSHPFPAWNEISGLPYLNACVNEALRIHPPFCLPFERIVPAEGMTIGD

HFFPGGTVIGMNPWVINRHRPTFGEDADAWRPERWLEDPARTRQMEN

TLLSFGAGRRVCLGKNIALLELKKLTSALVLHYE

LEIVNPEKFQSQNFFFFKQEGLYAAVKRRSAGSPELYPDDAVPH

 

>CYP631B2  Aspergillus flavus

100% to CYP631B2  Aspergillus oryzae

AFL2G_12402

MLILLGLLCLYTGLYVARTYWRLRHFPGPL

VARFTDLGRLWWVKTSRSHHHHMGLHSRYGQYVRLGPNMISISDPDAIPLVYPIRPGVPK ()

SDFYRSMMPYTRKGRSLPLVFNTRDEDLHKRLKTPIAHLYSLSNILTFEAFVDQVLEILFRQFEERFVPDQAPFNLGNWLQYFAFDV

MGTMSFSRRYGFLEKGRDDTGLLSAIWAFMKAAAPVTQMPWVDLVWNKNPFIALFRATPAQPILNVVLSRINDRRNELYS

TTSTPEKVNERDFLSRFMHIQSNSDTIPPWAVTAWSFSNVIAGSDTTAVAMKTLWYNLLLHPATMHRLRKELVQAQQQSK

LSHPFPAWNEISGLPYLNACVNEALRIHPPFCLPFERIVPAEGMTIGDHFFPGGTVIGMNPWVINRHRPTFGEDADAWRP

ERWLEDPARTRQMENTLLSFGAGRRVCLGKNIALLELKKLTSALVLHYELEIVNPEKFQSQNFFFFKQEGLYAAVKRRSA

GSPELYPDDAVPH

 

>CYP631B3P  Aspergillus oryzae

           GenEMBL BAE56559.1, AP007152.1

           52% TO 631B2, 56% to 631B1

           pseudogene

24632 NLLTHPATLQALSSELITANLTLPYPKWNEVCDLPYLDACIQEAVRLHPPF

ALVLGRVVPAGGVTVLN

HYLPEGTLVGGNPYVVNRHAETFGPDVEEWRPERWLEGEGRKRLEQSVPT (0) 24276

FDAGRRVCLGKYIGILELKRLVPFLVLKYD (0)

MKIIDPERFSVENGFFFKQRGFYCNITRRKEGSDRDKADPK*

 

>CYP631B3P  Aspergillus flavus

100% to CYP631B3P  Aspergillus oryzae

LFYNLLTHPATLQ

ALSSELITANLTLPYPKWNEVCDLPYLDACIQEAVRLHPPFALVLGRVVPAGGVTVLNHYLPEGTLVGGNPYVVNRHAET

FGPDVEEWRPERWLEGEGRKRLEQSVPTFDAGRRVCLGKYIGILELKRLVPFLVLKYDMKIIDPERFSVENGFFFKQRGF

YCNITRRKEGSDRDKADPK

 

>CYP631B4 Talaromyces stipitatus

ABAS01000029.1 61% to 631B1, 44% to CYP5077A1 note this might be misnamed

        MDSMNIFPGYRQADSIVFIAVGSILGLSL

285172  LHLLRTYWRLRYIPGPFLARFTNLQRVYWVKTTRAHEIHQQMHEKYGNVVRFGPNMVSLG  284993

284992  DPALIPSLYPIRPGFPK (0)

        SDFYRSLMPYTRKGGSLPAV  284813

284812  FNTRDENLHKVIKTPIAPLFSLSNILPLEVFVNRVLEVLFQQLDTRFVSSGDTFDLADWL  284633

284632  QYFAFDVMGTLTFSKR  284585

        YGFLEHGRDVNNMLLTIWTYMSNCAP (0)

MTQIPWFDVVWNKNAFITLFRRASGLSILGHVGELIA

DRRQRRNTPSAVSDEKASDRDMLSRFFELQEKDSKIPRW (2)

AVTAWAFSNVIAGSDSTAVTMRTTWFGLLSHPETLKTL

RKELLDQDSKLEGGITRPFPAWKDICNLAYLDACVNEAVRLHPPFCLPFERVVPSGGLTIGGTYFPAGTVVGM

SPWVINRHRPTFGHDAESWRPERWMVPEEHRRKLEQSVLT (0)

FGAGRRVCLGKHIAMLEIKKLTAALALNYE (0)

FDLLDPKRFKVENGWFFRQYGMDVKARKATVWEEDKKQ*

 

>CYP632A1 Fusarium graminearum FG03264.1 AACM01000150 FGcontig1.150_scaffold2

MTISFFATRDNKVHRFLRSRVASTYSMTSILSMESLIQDVLDLNLKKLGE

RADEGKKFEVDKTVNYYTSDVVSQLALGGMVGCVEQQKDIGGIIQSVHDG

FYFMGNLGVLPWQMFWINNPVSKWLTKNFGGDRLNGFDIFLDWLDNRVEE

RMINGLLPNQRRDILQNFIEAKDPHGQPASKEEVMIEGVNVLAGGSDTTA

FGILSCLRHLLTSPDAKAKLMEEIDHAYEELGLTKEGREISFKQAEKLPY

LSAVITESNRVQPSIQYQLPRTVPSEGAQVGDYFLPPGTTCGTSSRAVNC

SREIFGPDAEEFRPERWIAQGPDDEARIRKEKSLLMTFGMGSRSCIGKNL

AIVELHLYIAQFFRHFDAEVAKSAKPWGIKSQWLTFHHDFVITITRREH*

 

>CYP632A2 Nectria haematococca

e_gw1.80.13.1

Necha1/scaffold_80:8615-10329

70% to 632A1 F. gram.

MQHLFPRSALFLLLHSKGSLVILLVGLLFFRVLYNLFFHPLRKIPGPFLARSSELWRAIRYFRGTWHDDV

LELHKTYGQVVRISPGEVSFTSPEAIKAIYGHGKNVSKAGTSPLLHSLPRNNMLMR (2)

FFATRDNKVHRSLR

SRVSSAYSMTSILGMEPQIQDVLDLNRRKLGELADGANYIAFDKMVNYFTFDVVGQLALGGKIGFLQQGK

DVDGIIQSVSDGFYLMGNMGIFPLQMLWINNPVSKWFTKTFGGDRLNAFDVFIDWLDKRVEERMTNGLLP

GQRRDLLQHFIEVKDAQGRQADKKEVMIEGVNILAAGADTTAIGILACLGYILTNPRSKTKLTQEIDNAY

QELNIDDERREISFKEAEKLPYLSAVILESIRLHPSIQYQLPRNVPLEGAHIGEYFLPCGTTCGASARAV

NCSREIFGSDAEEFRPERWIPRSPEDGERIKSEKSLLMT (0)

FGMGSRNCIGKHLATVELYKYIAQFFRYFDA

ELANKAKPWNTKTQWFAFQRGFLVAIKRREHLADVEGPVGNC*

 

>CYP632B1 Mgr019 Mycosphaerella graminicola

39% to 632A1, 40% 632A2, next best hit is 30% to 5078A4 yedllow from JGI model

e_gw1.6.392.1|Mycgr3

TESVFATRNHQDHQFLRKRVFKTYSMSSILSMEHLIDEVVDLSLRQLHCHVMDNRPVRLDRWTMFFAYEVVSNLALGHLK

GFLERGEDVNNIIDSNHTGFWLNANVGYLPGQSRWFSNYYFLWVMTKLGSNFSAISDWIDGAVANGRRGHSSISRGPDLL

QHFLNMSDGGGKPVSDEEVASEIGNVLGAGADTTGILIMATIKYLIENQSDYCRLQREVDAEHRRLQLSAGQEIRYTDLA

KLPFLFAVVQESLRLHPSIVYQLPRESLKSGTTIRDQLIPPGVSISVSAAAVNRSVDVFGSDADAWTPQRWMEQNAGGGG

DSSFTLESSLMTFGRGSRGCIGKNLALVEAYKYTASFVRNFDASFIDPKKNGTVISYWFAKIEGVDVALTSRLA*

 

>CYP633A1 Fusarium graminearum FG03796.1 AACM01000164 FGcontig1.164_scaffold2

MAANPSGFLSFIAEDLQQSPGNYFTVLGVLFVTVLLHRLSTPSLETG  EPP

LLKPKFPIVGHFYGLMKYQNVYLKQLYDKYHRQIATLPILGGKLYVIFDP

AIVQSAYRKKTLSFEPFAAEFAQRELLLSDRVQDKLKNTNLVPDFFAAIH

PAMTGDHLHRMNANALNYISKDINKISGQGSLEHANLWLWLRDLVTMATS

EALYGSKNPLRQDRSLLEDVWTFEAGLNMLLVNIFPAITAPKAHQARARL

QAALGKYYGARSYEDEDAAEIVRGRAGAFVKYGVPDEEVGHIELALLHVG

TANTIPTLYWFFANVFNRPDLVLKLREEVSQVVQKDGGNKVVIDVSILDE

KCPLLVSCYRESLRVSNQATGNRRVLEDTTVTDNKGNSYLLKKGENVQVS

AAVSHNLDAWGEDASTFRPDRFIETKDSNDAEKTRRSAFIAFGGGRNLCP

GRNFAFAENLGLVACLLAGFDVTLPDGPKLPASVDCSFAQAAVRPAKDGA

GFGVRIERRLGWQETQWSFKS*

 

>CYP633A2 Nectria haematococca

fgenesh1_pg.scaffold_48000021

Necha1/scaffold_48:55055-56793

55% to 633A1

This gene model seems correct

MAQNVTTASNGFLSFVAEDVKQSPARYLTGLALLLSAIFLQKLSSPGRDEREPPFLKPSIPIIGHIIGMIKHHGGYFQILF (2)

DKTRARIGTLPILGGKLYIIFEPSMVQSAYRNKNLAFEPFAVEFAQRELAISNESARIV

RETNLVPDFFSVITKSMAGEYTHRMNANALKYVSKELEALGSNEPSKIPNMYLWVRDMMTIATTEALYGP

DNPIKGHPGLQEDLW (2)

TFEAGLTGILLNVFPSITARAAHYARARLQAALGKYYGAMKDQHEDAAQIVKSRA

GVLRSYGIQGAEVGTFELALLHVSTANTIPTLFWFMAQIYARPELVSRLRDEVVPAAQYGDDNEVTIDIT

TLDQKCPLLVSCYREAIRLSNQTVGNRRVMEDTTISDGKGNSYLLKKGLNVQMSAQVLHTLHSVWGNDVM

EFDPERFIEKGGKENQQSDRSKRTSFAPFGGGRHLCPGRNFAFAENLGLMTCLLLGFDVSPLDEDYTTFK

VPTMKACVFSEAAGKPEKEGEGFGVQIQKKKGWEKTKWRFVS*

 

>CYP633B1 Fusarium graminearum FG08207.1 AACM01000329 FGcontig1.329_scaffold5

MALTEGISELLLSKPVVLGLAACALLFLIGRAFEPTVDALEPPMLRPSIP

IFGHFYSMMKDQEAFFKRLDKKYHMPIATVPILKYKMYAITDPILVQSAY

CNKNLSFTPFAINGAQKVTGFDDDYHKVLMETNVLPEYFKSLYDGTTAQH

IHQLNVTSLKHVSQHINSIQENGMTIDNTYLWLRNLMTVATSYLFFHIFN

SKTLQRTKEARRRIQLALGKWCTNMRQDDERVSAYIRNRVGILRNYGVEG

QKLGDIEVGLIHVPTSNSIPTLFWFFMHVFTRPDVVEQMRAEVEHIVQRG

PDETVTVNIDDILERCPLMISAYREASRLCNGFTCNRIVMEETTITDRHG

RSYLLKKGSSVKMPAGVMHASQEVWGEDAAVFRADRFLDKGLTSEQAKLR

RAALTPFGGGAHMCPGRNFATAEIYGFMTALLLGYNVEPVDGKWDAFKPP

PMATCPQSTSVCKPEDEASVCGTRLIRRSGWEGAQWKFVSGKVTE*

 

>CYP634A1 Fusarium graminearum FG02872.1 AACM01000142 FGcontig1.142_scaffold2

MDSHSSSFLGQTFLDLRNGDLSSSSAWTTAAIATAIALSLLNYFLTPRLD

PREPPVVKPTIPWIGHILGIIRHQADYGRLIHNANPNHQIATLPMLNGKL

YAVFDPSLLQSLLRNKTASFEPFAIDYAKKTFDLTQEEFLKVKAPGVYDE

FTDAIHASFQTVSLHQMNVHFLACISAKLDPMSNGTMRAHADTHGKEKVT

NGQLQVENLYLWCRDVMSLATTKALYGDTDPFGSKPDLIEDMWCFEESVP

YFLLSLYPSITMPKAYKARSTLQDIVCKWYSEDHDVTDPSVSAIVRNRAG

SLRKNGLIGSEIGKFEVILPNVATLNAVPTFYWLLLYILDRPELVARIRS

EAEAAAVIAHDNGKKTVTFNIAEYEAKLPLLVSCYRETMRLVNQSVSMRR

ILEDITVTTPEGNTYLLKKGTDMQLPAGVAHYEQSVWGSDVNTFDPERFH

PGSKGSPEEERKRKAAYIPFGGGRHLCPGRNFAFAEIIGFASSLLLGFDV

EATGMGFGDMKKLGPQLAGGTVRPEKYGSGLGAQIKSRQGWGNVQWKFEC

*

 

>CYP634A2 Nectria haematococca

e_gw1.26.228.1

Necha1/scaffold_26:232434-234178

64% to 634A1 gap in seq after ISFGT (remove intron 2) add back cyan region

MGVLKQDSILQGLVADLVDGNYSTAAVWITALLAGALALSLLLTPRLDPREPPVVKPTIPLVGHIIGIIR

HQSDYHRIIH (2)

NANPTKPIATLPMLNGKMYTIYDPHLVQTALRSRIASFEPFVIDFAQKTFSLSKETFAKV

RGPNVFNDFGDAIHSSFQAPMLHKMNVHFLASISAKMDPISFGTVRVDEVNSGRE

ELVDGGLRVENLYLWCRDVMTLATTKALYGDHDPFGPQPHLVDELW (2)

LFEESVPYFLLSLFPSITMPKAYRARSMLQALMGKYYTEEHDINDPTTSQ

LVKNRAGALRKHGFTGNEVGQLEVILPNVATLNAVPTFYWMLLFIFERPELVNRLRKEVEAAAVVSNSEG

KRTATFDIASFDARLPLLISCYRETMRLSNHSVSMRRIMQDMTITGADGQSYLLKKGVDLQLPAGVTHHD

RSAWGKDFDEFVAERFLQKAGDAETERRRKAAYFPFGGGRHLCPGRNFAFAEILGFMAVLVLGFDVEPLG

MRFGEMKMLGGQLASGTVRPEKHGKGLGGKITRRSGWDDVEWRFEC*

 

>CYP634A3P Fusarium oxysporum

75% to 634A1, pseudogene fragment, no upstream P450 seq.

FOXG_12544, FOXG_12594, FOXG_17081, FOXG_13997

ARIRAEAENAAVVEEKNGKRSATFNIAEFEESMPLLVGCYRETLRLVNQTLSTRRILQDTSVTTPEGATYI

LKKDTDLQLPAGVAHYEDSVWGADVNVFNPERFLPSSKGSTEDERKRKAAYIPFGGGRHLCPGRNLA (?)

AEIIGFASALLLGFEIEAVGMGFGNVKMLGPQLAGGTVRPERYGAGLGAEIAMRQGWEDVEWRFEC*

 

>CYP634A5 Fusarium oxysporum

75% to CYP634A1

FOXG_15710

MTSQSLLTSLVDYRGGNVTILSTTVLAVIVAFSLLNRLLTPRIDPREPPVVKPSIPWVGHIIGIIRHQADYSRIIHNENP

HVQIATLPMLNGKVYAAFDPALLQTLLRNKTASFEPFVFDYAKKTFALKHETFAKVKVPGVYDEFTEAIHASFQVRHLHQ

MNVHFLGSIAAKLNHATIRADSINAGKETVANGRLHVKNLYLWCRDVMSLATTRSLYGDTDPFNRDPSLIEDMWLFEESV

PYFLLSLFPSITMPKAYKARSTLQNIACKWYSEDHDIHDPSVSALVRNRAGALRKNGLTGYEIGKFEVILPNVATLNAVP

TFYWLLLYILDRPDLLARIRAEAEGAAVIEDNNGKRSATFNIAEFEEKLPLLVSCYRETLRLANQTLSMRRILEDTSITT

PEGTTYILKKGTDLQLPAGVAHYENSVWGADVNVFNPERFLPSSKGNFEEERKRKAAYIPFGGGRHLCPGRNLAFAEIIG

FASALLLGFEIEAVGMGFGDVKMLGPQLAGGTVRPERYGAGLGAEIAMREGWEDVEWRFEC*

 

>CYP634A5 Fusarium verticillioides

93% to CYP634A5 Fusarium oxysporum

FVEG_13169

MNSQSLLTSLVDYRVANVTILSTTVLAAIVALALLNRLLTPRI

DPREPPVVKPSIPWVGHIIGIIRHQADYPRIIH (2)

NENPHVQIATLPMLNGKVYAAFDPALLQTLLRNKTASFEPFVFDYAK

KTFALKQETFEKVKVPGVYDEFTEAIHASFQVRHLQQMNVHFLGSIAAKLNTATIRADAINAGKE

TVTNGRLHVENLYLWCRDVMSLATTRSLYGDTDPFNRDPSLIEDMWLFE

ESVPYFLLSLFPSITMPKAYKARSTLQNIACKWYAQDHDIHDPSVSALVRN

RAGALRKNGLTGYEIGKFEVILPNVATLNAVPTFYWLLLYILDRPDLLTRI

RAEAEDAAVIEENNGKRSATFNIVDFEEKMPLLVSCYRETLRLANQTLS

MRRILQDTSITTPEGTTYILKKDTDLQLPAGVAHYEDSVWGADVNVFNPERF

LPASKGSAEDERKRKAAYIPFGGGRHLCPGRNLAFAEIVGFASALLLG

FEIKAVDMGFGDVKKLGPQLAGGTVRPERYGAGLGAEIQMRRGWEDVEWRFEC*

 

>CYP635A1 Fusarium graminearum FG08191.1 AACM01000329 FGcontig1.329_scaffold5

MSLTSNSKQRKEPIVTLPMLNGKLYVINSPDLIQAALRNNDISFTPFILE

SSKAMWGLSDNAMASISDLANLKGGMQIIHSTLGGESLHKLNISSLSRFM

TYLNRVKPGENIGIADTYIWLRDMLTDASATAVYGPKNPITVDKMHLVWY

SLLQSIYSTCSNSGRDYDKQALLVAIGLPSFVTKAAINARLKVNNLLLSY

YKNGGNHEKGASEIIQQRATYLRKTGFTDDDLSHMEFMILWVGVTNTAPV

LFWLFVHVLTSAGYTSRVRAEIEAITIITKTPEGRKATFDTRLLEKSCPF

LNACYQECLRHYSHSIGNRRVMQDTEIQDSQGRKYLLKKGVNVQWPPPVT

HFNTEVWGQDADVFRPERFMDVTPQDEKKRRGALLSFGGGKHLCPGRKFA

YTELLGLVGVVALGFEVKGLELPESKYAGIGIGGKMPDWENMEKGFGLRR

REGWEDVTWVFDGDN*

 

>CYP635A2 Nectria haematococca

e_gw1.25.117.1

Necha1/scaffold_25:315985-317594

52% to 635A1 gap after HLLW but this seems correct.  635A1 may be too long here

This gene model seems correct

MSTAGLAVALTGLLYLLKACLFPTMDPLEPPLLKPRVPIFGHIISMMREKAGFYTRLF (2)

KERRMPICTLPMLNGKLYVINSPSLIQAALRNNDISFDPFIVEFSKGIFGQNERQVEVIARPTVMKDLLDIIHSSLLGEPLH

RLNVVALEKMMGHINVIQPNESVAIPDAFIWIRDMTTEATAVALFGKKNPITPEHVHLLW (2)

TFDKSATLLAINVAPNLIAGESVAARSELNKLFLPFYEARCEEGPDVSDIIRKRTAVLRAAGYNDQDLAVQELMLPWVGS

TNTIPTLFWLFVNLFARPGYLERVRAEIEAITTIEDGPNGRTALVDSTKFEKHCPVLNACYQETLRMYLH

SVGNRRAMKDTKIQDLDGREYLLKKGTNVQWPPGVTHFIDTVWGNDADTFNPERFIKPTAQDERARRGAL

LPFGGGRHLCPGRKFAFAEILGFTGVLALGLTVEGLDLPDSTDPIFGSAPRRPVWGANGPGFKLSRRPGW

EDVTWTFKK*

 

>CYP635B1 Nectria haematococca

fgenesh1_pg.scaffold_35000113

Necha1/scaffold_35:263720-265404

46% to 635A1 gap after LHLI, this seq seems correct, 635A1 may be too long

This gene model seems correct

MAAAGQSVPMGASSTSVSARWLFGGEKTPSAVVLTAAIILPLLFVGL

KKLLYPIFDSREPPVLRPRIPFIGHIVDMIRERSSWYIRIY (2)

KENPLPICTLPMLHGKMYVINAPDLITAAMKNGDISFDPFLLEVPVGLFGLSKKLSDIIEQRHVIDGLLN

VIHYTLMGDHLSRMNITALTKMMETLNDVGPHPVELSDVYEWSWDVLGNATMVAIFGEKNPLTPEHLHLIR (2)

DFDEGIARFAIGVAPRLIAPGPLRAREKINTLLESFYAAGYETRPDVSSII

QKRTEILRREGFNDRDLGIQEITIPWVATTNTIAVLYWLFAHVFSVPEYTQRIRDEMEAISVIANTNEKG

RVGTISVKEMNKECTFLYACYRETLRLYIHNTGQRRVVKDTTIKDQGGREYLLKEGINVQWPGSLTHMTD

SVWGDDAWTFRPERFLEVSPQEEKQRRGAYIPFGGGKHLCPGRNFALSENMGFVGILALGYNIEGVRVPK

SEDPGIGLGSRKPIGGAALRSAKVSRRVGWEDVTWEFVE*

 

>CYP636A1 Fusarium graminearum FG02111.1 AACM01000111 FGcontig1.111_scaffold1

MGVNNATLGLVCCVIVAVVALATRKGPDSREPPYVKERVPYFSHIYGLLK

HGLRYFDVVSAQQPHPIFTIDMSGQKNYIVTSPELVQAVQRNTTSLSFSP

AMIPAFRRMMGFDEAGIELIFRDAHTEKGMYGEIHRVQKASLLPGTESLD

ELCTIIRGKLLTIVNDMPSSQTIDLYAWVQDLYMRTNNSACFGAKDPFTL

NPSLISTFWLWEANIKVLLLGIPWFLSPSKYSTAQRTRNDLVNAFTQYLG

NDGLETACSFIKELSNLGIRRGLSTENNARALVGSILAIVGNTIPTTFWL

LIQIFSRPDLLKEIRSELEATLEDPSSRSEISLNYTVIREKCPVLMSTYE

EILRMTSGIATVRYTNEDTLIQDRWLLKKGAQVQMPTAFIHADPTTWGAD

AEVFDHTRFLKSKVLTKEQKARRAAAFRPFGGGNTLCPGRHFASYEVLTF

AGSILLGFDMTPTTEAFNLPEMDRSKLPLTSLKPAGDIKVNLTRRSGWEK

VQFK*

 

>CYP636A2 Fusarium oxysporum

86% to 636A1

FOXG_04458

MALSNLTIGVVCCVVVAIVALATRKQPDAREPPYVKETVPYFSHIYGLLKHGLRYFDLVSAQQPHPIFTIDMSGQKNYIV

TSPELVQAVQRNTTSLSFSPAMIPAFRRMMGFDEAGIELIFRDAHTENGMYGEIHRVQKASLLPGTESLDELCTLIRTKL

LNIINELPSSQTIDLYAWVQDLFMRTNNSACFGEKDPFTIDPSLNSTFWDWEANIKVLLLGVPWFLSPKKHSTAQRTRKE

LVDAFLKYLNDDGLDTACSFIKELSGLGIRRGLSNENNARALLGSILAIVGNTIPTTFWLLISIFSRPDLLKEIRSELEA

TIEDPASEIVSLDYTTIRERCTVFMSTYDEVLRMTSGIATVRYTNEDTLIQDRWLLKKGAQVQMPTAFIHADPTTWGADA

DVFDHTRFFKSKVLTKEQKIRRAAAFRPFGGGNTLCPGRHFASYEVLTFAGSILLGFDMAPTTKTFNVPQMDRSKLPLTS

LKPAGDIKVSMSRRPRWEKVQFR*

 

>CYP636A2 Fusarium verticillioides

92% to CYP636A2 Fusarium oxysporum = ortholog

FVEG_07578

MALSNLTIGVVCCVAVAIVALSTRKKPDAREPPYVKETVPYFSHIYGLLKHG

LRYFDLVSAQQPHPIFTIDMSGQKNYIVTSPELVQAVQRNTTSLSFSP

AMIPAFRRMMGFDEAGIELIFRDAHTENGMYGEIHRVQKASLLPGTESL

DELCTLIRAKLLNIVNELPSSQTIDLYAWVQDLFMRTNNSACFGEKDPFTL

DPSLNSTFWAWEANIKVLLLGVPWFLSPKKHSTAQRTRKELVDAFLEYL

NDDGLDTACSFITELSGLGIRRGLSNENNARALLGSILAIVGNTIPTTFWL

LISIFSRPDLLKEIRSELEATLEDSSSGTISLDYNTIREKCPVFMSTYDE

VLRMTSGIATVRYTNEDTLIQDRWLLKKGAQVQMPTAFIHADPITWGADA

DVFDHTRFLKSKVLTKDQKTRRAAAFRPFGGGNTLCPGRHFASYEILTFVG

SVLLGFDMAPTTKTFNVPQMDRSKLPLTSLKPASNIKVDLLRRRGWGEARFR*

 

>CYP637A1 Fusarium graminearum FG03498.1 AACM01000159 FGcontig1.159_scaffold2

MLREILYGAAGLVLFAYTLEWLFSMSDDPREPKRLQSKIPVFGHLIGMMK

YSSGYHGITSKQTDQEIYTVAIFNTKLYVAKTARLIPLIQRSKTLSFRPF

MQTAAKHMGDAKPATFEIFGSEWVDSFSHAHKHSLAPGPSLDEQNSRMGD

RALIDIEELLPREKNGMNKVSLLEW

VRYAVVQASACGILGVEHPFRDPKIDAAFW (2)

KWQDYMPLHLINLD

ITKKGYAAREIVFDAFRKYNTNIPTDVSAVYMARQRTMQEAGIDEDDICK

QQATFGTAAFANTVPILYWTIYELFSRPELLEEVRREVIEHAVSRNKETG

FVVDVAALKTQCPLLLSIYQETQRTRHVHANIRKVTEDTLLDDKYLLKAG

QFVMMPGHPVHTNQTTWGESADVFDPYRFMPKDGSDKKAIASSSFMAWGA

PPHLCPARQFASTEILIVVALLVVRCEMSPASGQWVRSPTLNTGDMATIY

TPKKDVELEVRRTEEWDGEWSLRMGGSKTRVSLASG*

 

>CYP637A2 Fusarium oxysporum

78% to CYP637A1

FOXG_16528

MFREIVYGVAGFVLLAYAAEWILSLFDDPREPKRLQSKIPLFGHLWGMMKYSSGYHGITR (2)

SKQTNEEM

YTVAIFNTKLYIAKTSRLIPLIQKTSKTLSFRPFMQTAAKLMGDAKPETFEVFGTEWVDSFSHAHKNGLATGPFLDEQN

LRMGDRALIDIEQLLPVEKDGVAKVNLLEW

AQYAVVQASACGIFGVEHPFLDPKVDQAFW (2)

KWQSYLPLHMVNLDITGKGY

AARQIVFDAFRKYNKNLPSDVSFIYKERLRSMQEAGIDEDDICKQQATFGTAAFANTVPIMYWTIYELFSRPDLLEEVRK

EVIEQAVSGDKESGFKVDVAALKTKCPLTLSVFQETQRLRHVHANIRKVTEDTLLDGKYLLKAGHYVMMPGQPVHTNTST

WGPTADQFDPYRFTKDSSDRKA

SSFVAWGAPPHLCPARQFATTEILIVIALLAVRSDIAPVKGEWVKNPALNTGDMATVY

TPKKSVEVEVRKRDDWDN*

 

>CYP637A2 Fusarium verticillioides

97% to CYP637A2 Fusarium oxysporum = ortholog, C-term is extended

FVEG_13702

MLREAVYSIAGFVLLAYAAEWILSLFDDPREPKRLQSKVPLFGHLWGMMKYSSGYHGITR (2)

SKQTNEEMYT

VAIFNTKLYIAKTSRLIPLIQKTSKTLSFRPFMQTAAKLMGDAKPETFEVFGTE

WVDSFSHAHKNGLATGPFLDEQNLRMGDRALIDIEQLLPTEKDGVSKINLLEWAQ

YAVVQASACGIFGVEHPFLDPKVDQAFW

KWQSYLPLHMVNLDITGKGYAARQIVFDAFRKYNKNLPSDVSFIY

KERLRSMQEAGIDEDDICKQQATFGTAAFANTVPIMYWTIYELFSRPDLLEEVRK

EVIEQAVSGDKESGFKVDVAALKTKCPLALSVFQETQRLRHVHAN

IRKVTEDTLLDGKYLLKAGHYVMMPGQPVHTNTSTWGPTADQFDPYRFTKDSSDRK

ASSFVAWGAPPHLCPARQFATTEILIVIALLAVRGDITPVKGEW

VKNPALNTGDMATVYTPKKSVEVEVRKREEWD

GEWSLRMGESKTRISLASG*

 

>CYP638A1 Fusarium graminearum FG02114.1 AACM01000111 FGcontig1.111_scaffold1

MSILNSKLSFDLNSTTMKMNHWLETSPMAYILLSLLVLLFSTYFITTVRF

SLQRLSNRRYQGKEPSTLPYGVPGLGSALGLIRNPHGFFGSIVKNFDSGE

PIRMRLGLDHY YLIHGTRSVQHVFRCSKELTFEEFALRVAHKVKRLPERD

VALCSADKSGSARVPLIEAREEDRIWRKFHELYEHHLIGSSAVSGLTELF

VGLMNNELSTIALDGPIEVGIDHFLRKHMFRASTIALAGHGVFEVDPNFA

DVFWEYDVDFMPLLYGLPKIMCRKGWNARDKCLETTKTYLGNAWRNLDWR

AASEMNPEWEPNFGSRLVREREVAMEKYGLSLEGRASFQMGLIWSINSNA

IPMTSWIIIEILRRPEIFKRIKEEVATVFDKETKNIDIAEVRKLPLLNSV

YLECMRLRSSVFVVRKLRESIDLDGYTLKKGNLILAPSYLAHSAPEVWSS

GAHPPEEFWPERFIQKGSAGITAGNYFPYGGGVAMCPGRNYAKQEILAAA

TLFFAHFDAEPLRFVDDNGKTSDRGPEVGNEARG VARVDRDMVVRLWRA*

 

>CYP638A2 Fusarium oxysporum

77% to CYP638A1, one frameshift

FOXG_04461

MMDAPFFNASAST

IALVAVLLLLSTHCITTIRYSIQRFCDRRYQGKEPSTLPYVLPGVGSALSLIRNPHGFFNSIV (2)

QKVENGEPIRMRLGNVHAYLIHGTRNVQQVFRCSKELTFE &

EFALRVAQKVKRLPAKDAALVAKDISGSSRLPLTETREEDRIWRKFHEIYESHLIGANAVSALTELFIDTMIDELGTAA

TQGPIEIGIDEFMKHHMFRASTIALAGRKVFDIDPNFADVFWDYDEDFMSLLYGIPKFLCRKGSNARDKCLETVKGYLDQ

GWQNLDWRACHEQNRDWEPNFGSKLVREREVAMEKYGIGLDGRASFQMGLIWSINSNAIPMTSWIIIEILRRPEIFKRIQ

EEVATVFDKDTKQVDIVALKKLPLLNSVYLECLRLRSSVFVVRKLRNSIELDGYTLKEGNLVLAPSYLAHNAPEVWSSSA

HPPEEFWPERFIKKGNSGISAGNYFPYGGGAAMCPGRNYAKQEILSAVVLFFAHFEIEPLRFVDREGKTSDRGPEVGNEA

RGVARVDRDLLVRLQRV*

 

>CYP638A2 Fusarium verticillioides

94% to CYP638A2 Fusarium oxysporum = ortholog

FVEG_07581

MMNAPFLNTSASSIALVAVLLFLSTHSITTIRYSVQRFCDRRYKGKEPSTLPYVLPGVGSALSLIRNPHGFFNSIV (2)

QKVDNGEPIRMRLGNVHAYLIHGTRNVQQVFRCSKELTFEEFALRVAHKVKRL

PAKDAALVAKDISGSSRLPLTETREEDRIWRKFHEIYEAHLIG

TNAVSALTELFINTMIDELSTVGTQGPIEIGIDEFMKHHMFRASTI

ALAGRKVFDIDPNFADVFWDYDEDFMSLLYGIPKFLCRKGSNARDKCLETVKGY

LDQGWKNLDWRACHQQNPYWEPNFGSKLVREREVAMEKYGIGLDGRASFQ

MGLIWSINSNAIPMTSWIIIEILRRPEIFKSIQEEVATVFDKDTKQVDIV

ALKKLPLLNSVYFECLRLRSSVFVVRKLRNSIELDGYTLKEGNLVLAPSY

LAHNAPEVWSSTTHPPEEFWPERFIKKGNSGISAGNYFPYGGGAAMCPGR

NYAKQEILSAVVLFFAHFEMEPLRFVDRDGKTSERGPEVGNEARGVARVDRDLLVRLRRV*

 

>CYP638B1 Histoplasma capsulatum G217B

ABBT01000036.1 Ajellomyces capsulatus G217B

38% to CYP638A1, ony 25% to next best hit

HCB00815.1 revised

65285 MLTFAAFILAVFVTYLFTLCRFHFQLRKNQRGQRQNPASLPYLLPWIGNGFQFACYPHALFHFAR (2) 65479

ARLGPSH

MYIITGAEYVKAAFGSSKNLTFDSRTIQIMEQVYGLPAGDAAPYKHSPGKAKELVSKLDQLYIANLANRECTAAMIRQFV

KSLNAQVGCLRNDKGTEVMIGTCLRRLMATASISALFGDAIIEKNATFVQDYWIFSKDFKRLLYGMPKLFCRKGVHARDR

MLKAIKAFILDALQDDVREMEPKSPTVESSPPYVSLVVRETNKFFTEAGFSVEGRASEALSLVFAINDNAIPTTIWMLLE

ILKSPGLHKQIYEEVQTSVVPDQNGNGRNCLDLSKLLTLPLLNSLYSECLRYHLSAQLARHLKNDIEIDGYLLKKGNLVV

APTLTAHREPEVWFRPNHLPSEVWPERFLHVRNIKPSNYFPYGMGAISCPGRVYAKTVILTSVATMLLTFDFEVLGFVDD

KGKACHRAPKPVAQGMGVSLPDREPLVKLKPRFSQGIM*

 

>CYP639A1 Fusarium graminearum FG03850.1 AACM01000166 FGcontig1.166_scaffold2

YGIYICLFILPFLVTYAFTVLGNTGYRDGTAAREPPKVPYWIPFVGNTIG

FAYDTERFLFSSLRKFGKVPLRIFVGAEQMYFIPHGQTIVELFKSSRYLT

TKTFGIMTVRDAFGLPQPDVEIYAGDNSGVDVKPAPGWEHVEQAKRFHFV

QHRQLNAYLSGNALNAIISKFTEGYSDQIAQDTTFQEDGWVEVDDLYGWF

KNHLLRATTVALCGEKFLELSPDFLEDFWSFDYHLPNLFRRLPRWVVPKS

YQARDRLLECLLRYHEYGQQNLDFTDQKLLEKDWTPEFGAKIMSTRQEMF

KNIGLSPRGGAALDVGMIWAVNANAIPAAMWMLLGILLDKNLTDRVMAEM

EPSFHEKSLSFDNDRLCSGPLLNSVYLEVLRVRVAAPVGRSSLIPNLKFG

KWQMKQDVGMLSTSWFGGHDPDFWNTGSTLPDGSEEHPVDTFWAERFLKY

EDDPTGGPVRNTNVKPSTRPSKRTADDDRKASVFTEGTQGYFYPYGGGTK

MCPGRFFAKQKLMAGVALSLRAYEIELVDPEAAAKVGPNMDYFPFGTIPP

KGKVPARIRRRKL*

 

>CYP639A2 Nectria haematococca

fgenesh1_pg.scaffold_25000008

Necha1/scaffold_25:22167-24012

64% to 639A1 some difficulty in mid region, gap after LRYH

MNATAITATASFVAETSSYGKVLVFLVLPCLLTYAYTSYGNRGYRHDLASREPPRVPYWVPVVGNTFGFA

FDTEKFLFSAI (2)

SKFGNVPLRLLIGAEPMYYIPHGQPILDLFKASRHLTTKSLGVMTIRNAFGCPPADVKI

YADDESGTDAKPAPGWEHVDPSQRLHFVHHRDLHTLLAGSSLHALEEKFVESLSRSISNDSKFKKDEWTE

VSDLYVLFRDYLFCAALEAFCGEKFLELSPDFPENFWVFDHHLPNLFKRLPKWLIPKSFRMRDKALGGVL

RYHRYGREQFDFTDDNVLKGEWTPQFGSRLMRARQKMFLTTGFSVQGAAALDLGI (2)

VNANAIPASMWMLLGVLLDDNIKDRLMAEIDPAFTTSSLSFNKDKLCSGPLLNSIYCETLRVRVAA

PVGRTSLIPDLKFGKWKLNQGVTMLSTSWIGGHDTKFWNTGREFPDGSVEHPVDSFWAERFLQYPDDPSS

GPVRNPSFKAPFKTKRRTPEDDRNAQVITEGTQGYWYPYGGGTKMCPGRFFATQELKVGVAIMLRAYDIE

LLDHEAAAKIGPNMDYFPFGTIPPNGKVAARIRRRKLE*

 

>CYP639A3 Fusarium oxysporum

69% to CYP639A1 Fusarium graminearum

FOXG_17188

MNATETAAANPGSATEAISYFKVLSLVVLPFIVTYVFTSFGNKGYQHGQSAREPPRVPYWIPYVGNTIGFAFDTERFLSS

ILSKFGKVPLRIFVGAEPMYFIPHGQPIIELFKASRHLTTKSLGVMTVRDAFGLPESDMPIYVDEDSQFGHVDPLKRFDF

VQHRDLHALLTGGPLNSMTAKFVEVYSDIIEKDTRLNEDDWTEVDDLYEWLKNNLLRAAITALCGDKFLEISPNFLEDFW

LFDYHLPSLFKRMPRWLVPKSYAARDKCVESMLRYHEYGNQLFDFTDEDGVVKKDWTSEFGTRLMSARQKMFQSVGMTPR

GGAALDLGLMWA (2)

VNANAIPAGMWILLDILLDKDLKDR

VMAEMQPSFIDKSLSFDIDKLCSSPLINSIYLETLRLRVASPVGRTSIIPNLKFGKWQFKKGVGMLSS

SWVGGHDPDFWNTGHVQPGGVEEHPVESFWAERFLVYENDPASGPVRPTASAEKPAKRTSEDDRKARSTIDGTQGYWYPY

GGGTKMCPGRFFAKQELIAAVAVALRAFDIELVDPEAASKVRPNMDYFPFGTIPPKGKVAARIRRRKL*

 

>CYP639A3 Fusarium verticillioides

90% to CYP639A3 Fusarium oxysporum = ortholog

FVEG_12894

MHQPMSASNLNPLNASRRFTMNAKTAAANPASATETTTYLKLVSLAIL

PFIVTYILTSFGNKGYQHGQSAREPPRVPYWIPYVGNTIGFAFYTEGFLSSI

LSKFGNVPLRIFVGAEDMYFIPHGQPIIELFKASRHLTTKSLGVMTVRDAFG

LPECDMPIYVDDDSQFGHVDPLKRFDFVQHRDLHALLTGGPLNSMTAK

FVEVYSDIIEKDTKLSENEWTEVDDLYEWLKNNLFRAAVTALCGDKFLEI

SPSFLEDFWLFDYRLPSLFKRMPRWLVPGSYAARDKCVESVLKYHEHGNK

LFDFTDEDGIIKMDWTPEFGTKLMSARQKMFQSVGLTPRGGAALDLGLMW

AVNANAIPAGMWILLDILLDKDLKDRVMAEMQPSFIDKSLSFDIEKLCSG

PLINSIYLETLRLRVASPVGRTSIIPNLKFGKWQMKQGVGMLSSSWAGGH

DPAFWNTGHVQPGGIEEHPVESFWAERFLVYENDSTSGPVRPTAAAEKPT

KRTSEEDRKARPTIDGTLGYWYPYGGGTKMCPGRFFAKQELMAAVAVALR

AFDIELVDPEAASKVRPNMDYFPFGTIPPKEKVAARIRRRKL*

 

>CYP640A1 Fusarium graminearum FG11108.1 AACM01000459 FGcontig1.459_scaffold8

MESMIITPEMNSTLKIADVQAHDLPLQHNFLSYLFGLLIATYIVWQYFLR

TGVTESACSEPPMLPYWIPVVGHTFSFLTNTHNTIMSGRSHFKSITHPFS

LLIGGRRTYVVLDPHYIGKVYKKTKDLVHEPFIDHLMMCIGTTQKTRDIM

WNTMIGDSSLTDSALDWLREEVSQSPSSQPFFDRFMMELDHGLQQGDPLT

TGRLREHNMLKFVETIIITVSTNSFFGKVLLKQSPEILDSFPIFDRHVWK

MVFRAPKFTFMTAHNAKGSVIDGLTKYFDLPQSERQDAASFILKSEDAMR

ENGICSREIAALLFKFFWGINGMPATLAFWFLARTVYTPHLWEDIRAEVA

PAFRNGIHSPPDIGYLKKCPKLNATFHETLRIHGGTAGFRQVASDTVIGG

FTFKAGSDVIMPYRQMHLDEGIW GQDAKTFDIDRFIHNPKLATAKTFK

PFGGGVTLCPGRFHAHRTALSFIAIVITRYDI

HVVGGCESRPFPHMNTRGPEVGVIFPVLEQVPQIIVKNVDIE*

 

>CYP640B1 Fusarium oxysporum

47% to CYP640A1

FOXG_13207

MSAITVTPGVNATIGVVDTEGPVFTLHYQILFRVLGTCLVAYVAWLYLLRIGALWNSQLEPPMLPYWIP (1)

GIGQNISFFIDVEKLLVAARLVLTGLSITV (0)

PFSVLIGGRRTYVILDPHDIAETHQKTKELQFDAYIDQFMEYVSVSKK

ARDILWGTTLSETSLSVPNSLRSWIRADMTQT

SSRKFYSEVLLELDSVLQQGSPFTTGKSSEHDMLKWTSDIIVKASTKSFFGNALFEKSPGLVDHFRRFNRQTWKLL

YKYPRFLSRTAYDSRDSAIDGLERYFKMPQDQRGDAAPFVIKAEDEMRKHGISDRDIAAVLFKLYWAINGNPSVLAFWLL

VRTLYTPNLKEDIKSEVAPAFKNGIHHQPEIEYLKECPKLNATYYEAMRLHSGSSSFRRVVQDTTIAGFQLKAGNDVMMP

YRQLHLNKKYWGENAENFDIDKFVDNPKLHSARTYKPFGGGTTFCPGKLLARQMALVYLAILVTRYDIEIIGGCEGQPFP

EANDKAPTLGIISPKPGHDVKILVRDVS*

 

>CYP640B1P Fusarium verticillioides

68% to CYP640B1 Fusarium oxysporum

no gene model, supercontig 19 373448 – 373945 region

IRADMTQTSSRKFYSEVLLELD

 

YPRSRSV*DSRDAAIDSLEWCFEMPQD*RRDAALFVIKAEDEMRKLGVSNGHISAVLCKLYQA

INGNSSVLAFWLLARSLCTPHLMEDIKRGVAPAPK

EGIHHQPDVE*LKECPKLNATYYETKPLHSGSSSFRRLVRDTHITIAGLRLKTGNDVIM

PYRQLQLDKKYWGENS*HLDIGRFIDNPKLYSTRECKLFGGGTTLCPEMTLSAASVYL

ATLVTLYEIQPVQRCESQPFPEANDKVRMLGIISPEPGYDVKVSMRYV

 

>CYP641A1 Fusarium graminearum FG10910.1 AACM01000454 FGcontig1.454_scaffold8

MDSLEALRAYLNENLIPHAQRYPLQAILVTTIVLFITTRLFTGSPSSVRK

DGSKTPPLAPFWIPLFGHAPRIFLNPSAALTRFRNRYGQGVFSVRLFQSI

HSFVFRPSLAARLLEQPDSVADKEFITRHIMMTNFGLSKKDLAAYDKAAG

EVYQVTKEHLSGSHLDDLAKATLRDLDDNAADVISFNCYSTDQMDWERLA

NAELLEDIKDENIMAVDFFELIKTFIARTATISVFGTDFVEAYPDIWPHL

WIFNNGFHSLAMGVPVWAPFPTSQRARIALGRLLTFMREFHDEFETFLSG

EEPGQKWQDFHTVSPLVRARAEVYRKHGLSSDVRAAFDVSLLWSTTVQTT

SLVSWSLFELYQDPVLLSQIREQITPCVQIVQPNHDFGGAVWIPPQIKKL

DLEGLLTKCPLLQGVYLETLRLYGGGWSARYLKEDFTLKDKEDSFVLKKG

TFAHVINNLHHSDPRCFTDSKIWQAGRYLEDTVDKKGVKTQKVDPYTVKA

SDGTLTMCDDSEFTLRKVLMYVSVFISLYDLQPAGSESWPSPSIVKGVAS

VQPWSSLRLWVKRRTPPKDN*

 

>CYP641A2 Nectria haematococca

e_gw1.11.569.1

Necha1/scaffold_11:397275-399029

64% to 641A1 F. gram.

This gene model seems correct

MNGLEAVRQRLEENVVPHLKETPLLAAIVTVLVLVVTTRLFTGGSSSQNGSRSPTLAPYWIPFFGHAPRL

FLSANFTLTRFRNRYTQGIFSIRLFQSIHSFVFRPSLAAKLLELPESVADKEYVARRLMVSNFRLSKNDL

EAYDKAASEVHQITKQYLSGSHLESLVKANVRDLDDVAAELVSFNSYQTDQMEWEKMAEADVVETETKER

VMEADFLELMRNFVARSATASVFGTDFVENFDDIWEHLWVFNDGFLSLAMGVPLWAPLPTSQRARIALGR

LHSFMREFHEDLDKFLNNEEPGMRWQDFHTISPLVRKRTEVFRKHNLSIEIRASLDVALFWSVTAHSTSM

VSWSLFELYQDPVLLEQIRDQVAPFVHVVQPKNEFGGVVWVPPKIEKLDMEGLMTKCPLLKAAYLETLRL

HGGGWSARLLKEDVVLKDEGEAGYVLKKGTFAHVVPDLHRTDPKAFSDQRIWHVGRYLEDDVDSKGTKIQ

KVNPKTVQAS (1)

DGTLTMCDDSDFTMRKVYMYTSVLISLYNVEPKKGGDWVTPTLVKGVASTRPSRSIRLWIKPRALPKAK*

 

 

>CYP641A3 Fusarium oxysporum

84% to CYP641A1 Fusarium graminearum

FOXG_12845 MDRLEALLERLNETLIPHAQRYPLQAILVTTIILFITTRLFTGSSSSSRKDGSKTPPLAPFWVPLFGHAPRIFLSPSSAL

TRFRNRYAQGVFSIRLFQSIHSFVFRPSLVARLLEQPESVADKEYVARRIMMTNFGLSKQDLAAYDKAAPEVYQITKEYL

SGSHLDTLAKATLRDLDDNAADAISFNSYPTDQMDWERQANAELLEDTGDEKIMAVDFFELMKTFIARTATISVFGTDFV

EIYPDIWSHLWIFNDAFHSLAMGVPVWAPFPSSQRARIALGRLLTFMREYHTELDKFLSDEEPATKWQDFHTISPLVRAR

TEVYRKHGLPIDVRATFDVALLWATTVNSTSLISWSLFELYQDPVLLSQIREQITPFVKIVQPKNDFGGAVWIPPQVQKL

DLEGLVTKCPLLQGVYLETLRLYGGGWSARYLKEDVILKGKEDSFVLKKGTFAHIVNDLHHSDPRSFTDAKVWQVGRYLE

DTVDKKGVKTQRAEPCTVRASDGTLTMCDDSEFTLRKVLMYISVFISLYELEPAGSERWPSPSVVKGVASAQPWRSVRLW

VRRRSPQPQ

 

>CYP641A3 Fusarium verticillioides

93% to CYP641A3 Fusarium oxysporum = ortholog

FVEG_11589

MDSLEALRERLNETLIPHAQRYPLQAILVTTIILFITTRLFTGSSSSS

RKDGSKTPPLAPFWVPLFGHAPRIFLSPSSALTRFRDRYVQGVFSLRLFQSI

HSFVFRPSLVARLLEQPGSIADKEYVARRIMMTNFGLEKKDLAAYDKAAP

EVYQITREYLSGSHLDTLANAALRDLDDNAADAISFNSYPTDQMDWERGA

NAELLENTGDEKIMAVDFFELMKTFVARTATISVFGTDFVEVYTDIWPHL

WIFNDAFHSLAMGVPVWAPFPSSQRARFALKRLLTFMREYHTELDKFLSD

EEPATKWQDFHTISPLVRARTEVYRKHGLSIDVRAAFDVALLWATTVNST

SLISWSLFELYQDQVLLSQIREQITPFVKIVQPKNEFGGAVWIPPQVQKL

DLEGLVTKCPLLQGVYLETLRLYGGGWSSRYLKEDVILKDKEDSFVLKKG

TVAHIVNDLHHSDPRSFTDAKVWQVGRYIEDTVDKKGVKTQKVDPYTVRA

SDGTLTMCDDSEFTLRKVAMYISVFISLYELEPAGSERWPSPPVVKGVASA

QPWGSVRLWAKRRSPQPQ*

 

>CYP642A1 Fusarium graminearum FG01583.1 AACM01000081 FGcontig1.81_scaffold1

MFDHTRTLNHISSFSLNIIPTLIFFLVAAYVLPIITRRLLRYVASPTSFS

EKSSGHGTPSISNLPHLSGVVPWLGHLMGLTIDSTRYINSLIASTTAPIF

TINIPFARITVCHPSMDRVLSRHVNDTGLAQVLVYVGPRLFGLKQDTIKA

VFGYNPQPLHKQKFGHAENIVSLNQRSSTNIRDRVAKMPEVNELLIGRWV

FELAVSATASAVWGVANPWSMDQEFSNEFMKLSETFDTLGRPFSWLTANS

AWKSRKFLLKRLREFHLQHREARVQTTGHSINVVASSDPDWENNPDYYHI

EMVSALGLLATTSTLAVWLTRHLLTDPDLVKFVLKEVEQVKYVEEDEDKP

RLDFANVRSECPWLMASWYETLRLHMTGVARIARHDFMLNIPGSEPIAVP

QGEIFMLPMCASNLDVDVWGLDAAVFKASRFIDKEGQVSGSAVRKVRAFG

VAGNMCPGRVFGTDIVFSVIGTMLRTFDIEAAPGEEFRVPTLRGGFNVGF

ERYGDDVKVLLKRKHTA*

 

>CYP642A2 Nectria haematococca

e_gw1.47.68.1

Necha1/scaffold_47:131858-133483

56% to 642A1

This gene model seems correct

MAVAVDVFAFLGYKTEFLNGRLLLSAVVDLALIFWFLSILSSIVHYLLPRLQSSFSFSKKSSHDGYLRIS

QLPQLSSPIPWFGHLLGLQRDTARYVNTVIASTAAQVFTINIPFKQIIVVNPSLDKQLARHVSDTGLAQI

LAYVGSRVFGLGPATIRVILDTDPRRLHQVEFGRPENLKALSECSGVFIWDEMNKMAPSTEVDLAHWMFG

LTVSATASAVWGVKNPWRMDREFAEEFM (2)

NLTETFDSLSRPVPWLTARSAYKSRDFLMKRLREFHMQHRES

RVKTVAHAINVVAHSDPDWETNPDYYNIEMVSAIGLLATASTLSVWLVRHLLAIPELLKVVVDEIQQLQV

VQAQTQDGLKLDLTNIRTLCPWLVASWYENLRLHMTGVPRLARHEFGLTLPGSEPLAVSQGDLFLLPMCA

SNLDTNAWGPDAATFRPSRFITNNGELSNSLTRKVKAFGVAGNLCPGRVFGFETAMAMVAGTLGTFEIKS

VDGKEFTVPGVRQGFNVGFERYADDVKVVLSKR*

 

>CYP642A3 Fusarium oxysporum

85% to CYP642A1

FOXG_11132

MSSILQTQALSSLTINLIPTFILFSVAVYVLPALTRRLLQFLSSPSSFKEKPFTNDTPSISNLPRLSGVVPWLGHLFGLT

IDSTRYINRLIKSTTAPIFTINIPFARITVCHPSMDRVLSRHVNDTGLAQVLVYVGPRLFGLKEETIKAVFGYNPQPLHK

QKFGHAENIVSLNQRSSNNIRDRVMRMPETNEVLVGRWVFELAVSATASAVWGVANPWSMDQDFSNEFMKLSETFDTLGR

PFAWLTANSAWNSRKYLLTRLREFHLEHREARVKTTAHSINVVAHSDPNWETNPDYYHIEMVSALGLLATTSTLAVWLTR

HLLTDPELMKIILNEVQQLKHVEGEDKPRLDFANVRTACPWLMASWYETLRLHMTGVARIARHDFMLNLPDSDPIAVPQG

EIFMLPMCASNLDVDNWGPDAAVFKASRFIDKAGEVSGSAVRKVRAFGVAGNMCPGRVFGTDIVFSVVATMLRTFDIEAA

PGEGFRVPTLRGGFNVGFERYGDDVKVVLRKKGVA

 

>CYP642A3 Fusarium verticillioides

94% to CYP642A3 Fusarium oxysporum ortholog

FVEG_09657

MSSILPSQALSSLTINLIPTLILFSVSIYVLPAPTRHILKFLSSPSSFKEKSSINDTPSISNLPRLSGVVPWLGHLFGLT

IDSTRYINRLIKSTTAPIFTINIPFARITVCHPSMDRVLSRHVNDTGLAQVLVYVGPRLFGLKEETIKAVFGYNPQPLHK

QKFGHAENIVSLNQRSSNNIRERVMKMSETNEVLVGRWVFELAVSATASAVWGVANPWSMDQDFSNEFMKLSETFDTLGR

PIAWLTANSAWNSRKYLLARLREFHLEHREARVKTTAHSINVVAHSDPNWENNPDYYHIEMVSALGLLATTSTLAVWLTR

HLLTDPELMKVILNEVQQLKHVEGEDKPRLDLTNVRSECPWLMASWYETLRLHMTGVARIARHDFMLNLPDSDPIAVPQG

EIFMLPMCASNLDVDNWGPDAATFKASRFINKVGEVSGSAVRKVRAFGVAGNMCPGRVFGTDIVFSVVATMLRTFDIEAA

PGEEFRVPTLRGGFNVGFERYGDDVKVLLRKKGVA*

 

>CYP643A1 Fusarium graminearum revised 2/21/06 FG02367.1 AACM01000117 FGcontig1.117_scaffold1

MTNSTERLDSKAIRIAPNELHIDDVKMYKEIYNQTATYTKQPDFYAGFGT

PHSDVFAEHDLNLHKQR*RRLNPYFSRRAIGGLENLLKD

KVEQLGKRIDKQNGPYNLFNAVRCTTVDIISHYCTGEPLGQLENSD

ENFNGDFLDAFDAVSVSLWKFMYQPVFRSLMFGIPASIAKIVSRDARLMI

KLYGECHKSALNFKQNTPKSSHGTVFSSLSDLDEKDMGDEAVDLLIAGSD

TTAYTLAVAVVQICQNTQVKKKLVESLNAGISDVKNLPSLLGLEQIAYLN

ATVREALRFAIAAPGRLPRVVPRDSKPLVIDDQVIPAGSIIGMSAYTMNF

SKELWGKDAAEFNPDWWLGEDGKSLDTNMCSFSKGLRSCLGQNLAFAEMH

YILAYLFAKYDMELVDKSDNIKVHDRFTTYVTNHAIMVDMKKHKA*

 

>CYP643A2 Fusarium graminearum FG01722.1 AACM01000091 FGcontig1.91_scaffold1

MNQTYVWAIAQTETLFQSHSRNSLWGYFLVISTAGYLLYWAFDRTYKLHM

HPLASFPGPKEACVSQKWLHRVSEEGSPERVFEKLHQKY NSRAIRIGPNE

LHIDDVTLYKSVYNQNATYLKHEGFYGGFNVPHTVFAEHVPSLHKERRRR

LNPFFSRRAIE KLEDLLKGKIGQLGKRIDSQIGPYNIYNAARCTTVDIIS

HYCAGKPLGQLEKSDQNFHGDFLQSFDSLSKVLWKFMYQPIFRKIITSIP

DSIAKAADSETRTMLTLYDACRAAAIEYKRSPPQSDHATIFSCLSDLREE

DMGAEACDLLIAGSDTTAYTIASSIFHISQNTSIKRKLVEALDAANINVE

ELPSLVELERIEYLNATVREAIRFAIPAPGRLPRVVPHDAKPLVVDGQVI

PAGTVIGMSAYTMNFSKELWGEDAAMFNPDRWLGVNGKAMESNMCSFSKG

IRSCLGQNLANAEIHYNLAYIFGKYDVDSVTKGDKLEVFDRFATHVPSHN

IMVDLRRRENSAR*

 

>CYP643A3P Fusarium oxysporum

71% to CYP643A1 Fusarium graminearum

FOXG_06961

MRNIVSLGLRPPHTLTLLTASRLDSRAIRIAPNELHIDDVNIYKDVYNQNSTYIKH

ADFYAGFGTPHSVFAEHDPKLHKQRRRRLDPFFSRRTIGQLEALLKEKIEQLGKRIDSQS

GPYNIFNALRCTTVDIISHYCVGKPLGQLEKSDRRFNGDFLDAFDSVSNILWKSMYQPTF

R*IFSSIPAPLAKAISKEACLMISLYSACHTAAISFKSSPPKSANATIFTNLSDLGEKDM

EDEAVDLLIAG*DTTAYTLAAAVVQICQNTCVKKKLINTLDAGVADVDNLPSLLELEKME

YLNASVREVVRFAIASPGRLPRVVPQGGKTLVVDGRVVPAGSIIGMSAYTMNFSKGLWGD

DAATFNPDR*LGDSGKSLDANMCSFSKGIRSCLGQNLAYAEIHYILAYLFTKYNIKLVDR

EADIEVHDPFTSYVTSHAIMVDLRRREVSNSLK*

 

>CYP643A4 Fusarium oxysporum

67% to CYP643A2 Supercontig_9 136491-

FOXG_06968

MNSSPLPTIPQIQALIHDSQRGDILWGLVISTVVYLIYLVIDRVYKLYLHPLSKFPGPTEACLSQKWLLRVSEEGNPERV

FERLHEKY (1)

NSRAIRIAPNELHIDDVKIYKDIYNQTSTYIKQPDFYAGFGTPHSVFAEHDPNLHKQRRRRLNPYFSRRAIG

QIETLLKSKIEQLGKRIDSQNGPYNIFNAVRCTTVDIISHYCVGKPLGQLENSDENFNGDFLGAFDSVSVV

IWKFMYQPFFRWLVSVIPPALAKAVSREARLMLSLYNACQQAAISFKRNPPKSTQATIFSSLSDLGEKDMGDEAVDMLIA

GSDTTAYTLATAVVQICQNSRVKQKLVESLDAGIADVKSLPSLLELEQIVYLNASVREAVRFAIAAPGRLPRVVPRDNKP

LVVDGLVVPAGSIVGMSAYTMNFSEKLWGDDAAEFNPDRWLGEGRKSLDTNMCSFSKGLRSCLGQNLAFAEIHYILAYLF

AKYDIELVDKPDDIEVYDRFTSTVIEVSAIAGRGVIAANVSVAAGASISARCKSAIDEAQEVIEDSQCD*

 

>CYP643B1 Fusarium graminearum FG04724.1 AACM01000196 FGcontig1.196_scaffold3

MDYSRVVIDYFQKIINYPPSQLACLITVIIAIATFQILRAVYRLHFHPLS

KFPGPRSAAISRQWQAKIVRKGFPEKDYEKLHKEF GTKVLRIGPNHLHIS

DPSVYKVIYSQVNAFPKEPLFYNSFESRHTVFSETDVQLHKERRKLLNPL

FSKAGVQKLEPLILEKIQETKEKIKRISKDGSINVWPALR CMTIDIVSEF

LFGSCINMINEHPTSFESEYLKAMSLASELPFERYYSTMQRVVAKLVPLS

IAANFNPVLRQTEKLVGIIIDSYDTYKRRTTRSRFPVIFDKLESLSADLQ

KAEAINTFIAGSDTTAFTLTTALFHILHTPEVEKTLTETVDQLFGESHDI

PSLIQLEQVKYLRACVNEALRLGMGVPGTLPRIVPKQAQPFVVEGKVIPP

GTLVGMSAYTMNTDPQIWGQDAKSFNPARWLGPDSKELEKQLCTFSKGAR

QCIGMNLAYAEITMTLAHFFHYFKMELKTKELLAEDRFVHDVVGPEETKN

 

>CYP643B2 Fusarium verticillioides

70% to CYP643B1, not found in the F. oxysporum genome

FVEG_12349

MASFLVITEYSPLAFLAPAAAALVTVQIVRATYMLYFHPLSKFPGPRSAAISRKWQAKIVDKGFPEKEYKRLHREF

GTRALRIGPNHLHISDTSLYKVIYSQV

NAFPKEKAFYDTFESHHTTFSETDAQLHKQRRRLLSPLFSRSGVSKLEPLILEKVKETIEKVQRISQRGPINAWPAFR

CMTIDIISEFSFGTCINLINEDPDTFSSEYLAAMEIAS

DVPFLRYYSTTQRLLAKFIPLSVAANFNPVLSQLQRLVGIIGHSYDNFTH

RTNDSRFPVLFDNLQAIPPDMQKAEAINTFIAGSDTTAFTLVTALCHILR

LPEVEKTLTKSLDEIFGEHQATPSLLQLEQTKYLRACINEALRLAMSVPGI

LPRVVPTRSQPFVVDGKVVPPG (0)

TVVGMSAYTMNT DPQIWGQDAHSFNPDRWLGPNAKELETHLCTFSKGARQCIGIN (2)

VAYAEVTIALAHFFYHFKMELKTKELRIVDKFTTEASDP

GLLVDFKLRQHA*

 

>CYP643C1 Aspergillus niger

fgenesh1_pg.C_scaffold_4000279|Aspni1

44% to CYP643B1

MRQAVQLTGADMATLYKYIVEQVAEAPAAKGLSIICCTAAVATLVIVALLKPLYYRTWHPLSRFPGPPAAASSRHWIYRV

TDRGFPEEDLEKLHKKYQTQALRIGPNELHITDVSKYKVIYSQSKPFPKHEEFYEAFNTPHTVFTEIDAGMHKERRRLLN

PFFSRAG VFKLEPIIHDKVDILINKIRRLENSHNINVYDAFR

CLTTEVIMEFAFARSASMLEESETTFESWFLVAFDAVA

RSLWKMQEWPIARKALGVMPVNVIGLLDSNIVNVLKMLKFAESCLKHYEEHGNTTSHPVVFENLSSLPHDLKVTEAVDIF

IAGADTTASTLTAGFMHILSNPAIYAKLADALRGMDITSNDPSAAQLQELEKIPYLTACVKESLRIAMPVPGRLPRVVPQ

DLAEPFVVDGQVIPPGTIVSMSTYTMHTNEEVWGPDARVFNPDRWLQPGAKSLEQYLCTFSKGARMCIGQ

NVAYAEVTIVMAYLFRNFDIQLPPGFRAPEKKDLFTMEYSKPGLPLRFSSLKAK*

 

>CYP643C2 Aspergillus oryzae

Supercontig 6: 919868-921806 (+) strand

MSNIANMADGLGVPVLKTQGASILITLVIGIGTLHIVRSIYRRHFHPLSQFSGPPEAALSTKWLYKTNQAGFPEHEFERLHEKY (1)

QTKALRIAPNELHLSDVHQYKVIYSQSKPFLKDPPFYSSFNIDHSLFAETDPALHKERRKMLNPLFSRAGIFKLEG

VIHTKAGIMMKKIDRLREKHLINVYDAFR ()

CLTTEVIMEFAFARSANMLEEEESTFDSWFLRAFDSVASDIWTAHEWPVLR

KIGSCLPKSIVKIMNKKVASFFEVIN ()

FAESCMNYYEKHGNTTSHPVVFDHLTSLSYPQKITEAMDILIAGADTTASTLTA

ALLHILADKKIQTKLVQALQSVQPNEQGILPLMELEKIGYL

TACVKESLRVGMPVPGRLPRIVPDNLAQPFTVDGKIIPPG (0)

AVISMSAYTMHYSEELWGSDARSFNPERWLQPDSKNLDQYLCTFSKGARMCIGQN (2)

VAFAEITIVMAYIFQNYKLSLPSDFQRPKQKDLFTMEYGKPGLPVKFEAVN*

 

 

>CYP643C2 Aspergillus flavus

60% to CYP643C1 Aspergillus niger

99% to CYP6343C2 Aspergillus oryzae

AFL2G_02642

MSNIANMADGLGVPVLKTQGASILITLVIGIGTLHIVRSIYRRHFHPLSQFSGPPEAALSTKWLYKTNQAGFPEHEFERLHEKY

QTKALRIAPNELHLSDVHQYKVIYSQSKPFLKDPPFYSSFNIDHSLFAETDPALHKERRKMLNPLFSRAGIFKLEG

VIHTKAGIMMKKIDRLREKHLINVYDAFR (2)

CLTTEVIMEFAFARSANMLEEEESTFDSWFLRAFDSVASDIWTAHEWPVLR

RIGSCLPKSIVKIMNKKVASFFEVIN (0)

FAESCMNYYEKHGNTTSHPVVFDHLTSLSYPQKITEAMDILIAGADTTASTLTA

ALLHILADKKIQTKLVQALQSVQPNEQGILPLMELEKIGYL (0)

TACVKESLRVGMPVPGRLPRIVPDNLAQPFTVDGKIIPP

GAVISMSAYTMHYSEELWGSDARSFNPERWLQPDSKNLDQYLCTFSKGARMCIGQN

VAFAEITIVMAYIFQNYKLSLPSDFQRPKQKDLFTMEYGKPGLPVKFEAVN*

 

>CYP643D1 Neosartorya fischeri

43% to CYP643C1

NFIA_045690

Note: this seq has no ortholog in A. fumigatus

MLDRFVNDVNHAEAPMWLRVLLDKSLLLIFISCVGVYAITRVLVTFYRLYLHPLAAFKGPRAAAISRNWVYKVLTGPSLP

ETVFENLHTEYGVKAIRIGPNELHLTDVSLYKTIYNQTTPYLKEHGFYDGFLTPHTIFAETDPQLHKERRKMLNHMFSKM

GVRKLEPVIQKKILTLGKKIKAIASSGPINVYDAYRCMAIDIMMRFAFGNESDAINETPGTFKATFLTAFDAASHTLADT

QENPVKRFVAGKIPLSLVAKVDKNIRVIYKLQDTAYQGIRRYKKLTESPSHPVIFDNLKSLTDKAQAAQSIEILLIGSDT

SAFTLSMGSFHILSSPECKEKLVATLNEHIPNPDAMPSLVELEKIDYLWAVVKEVLRMAISVHGRLPRVVPKEAPPLLVD

GKVIPPGTVIGMSAYTMHTSIELWVRTSAHSVLNAGYPQMELGILTGSRSICVPSARAVAYAEVTMALAYFFRTFDMTLR

TTKPELRGHFTLGFANGMMIDFKKSLRLYARNGLLLDLSLREFWGEVLSCQMMMFTTI*

 

>CYP643E1 Neosartorya fischeri

52% TO CYP643C1

NFIA_058010 REVISED

NOTE: THIS SEQ DOES NOT HAVE AN ORTHOLOG IN A. FUMIGATUS

MALSGLVSAKLVIWNLT

KKQSLIILSALVAVVFIFRSLRKLYLAHLHPLAKFPGPAEAASSTAWMYGVLKSGSPENHYEKLHELY (1)

QTKALRIAPNELHITDVHQYKVIYGTSRPFPKLPSFYNSFHTPHTVFVEIDPTLHKERRR

MLSPLFSRTGVFRLEPIIHSKVKELLHRIDQMTGKKVVNLYDAFR HVTADIIMEFGFGRSPSIPDQKGLGAKSWLIHALE

GFGRNIWTMQERPIINRISGMIPLCIMQYFDNGFGPMARLMKYGETCLQEYEKGEYAKSHPIVFDSLPTLSYKLKVMEAV

ELLIAGSGTTASTLTSAMIHILKNKDIHARLVSALQKVQPNEEGNLALSDLERVDYLVACAKESLRFGLAVPGRLPRVVP

DGLEQPFVVDGKVVPPGTIVSISAYTMHYSEELWGADARTFNPDRWLDSRSKNLEQYLCTFSKGARMCLGHN (2)

LASAEVIIILAYLFKYYNMSLQPEFESPKTKDNFVLEYCSPGLPVTFETCFQDGCCKGKQGAIPQ*

 

>CYP644A1 Fusarium graminearum FG10610.1 AACM01000444 FGcontig1.444_scaffold7

MDELKVLKIPGAVYVLAGACVLVYFIYQRAYPRPLAGIPYN

KHALKRFAGDLPEIQERQKDGASIRPWFLEQAGKHQSAIMQIFLGPFASP

AVLISDFREASDILMHREADFKRGKKVDVFKGVLPHAHPAMETWDPRFKN

TRDLVRDVMTPSFLHSVSAPKVREVSIKLAELWKLKSQFATGRPFDVAEN

IVEFSFDAILSAATGLGPSGGDVNQQVLHLAEVLKEKPLESSVDIHRPIE

VPALDRSTKLKSLVTDEETLWKGFYMPWPGLYHMVNGLRPQVKDARRVLR

NYINSQITDAIPRLQTDGKPECALDYVIQREIKAALKEGRAPVLDDPRIR

DQIYGYLIAGHDTSAGTLSWIIRRLMANPEEQVKLRSSLRETYRAAWDEK

RIPTAQELTSHAPYLDAFLEEVLRYNCPVVTIMIVARRDTIILGHAVPED

TPVFLNLTGASMSRPSVPVDESLRSKSSKSYKPARENWDDMDPEMFYPER

WLVEDQDGKKSFSASAGPTLAFSAGNRGCWGKRLGYLELRVVLSILIWVF

NFEEVPEEIVNWDTYDSLVTAPKECFVRLTEAYPGAI*

 

>CYP645A1 Fusarium graminearum FG00127.1 AACM01000005 FGcontig1.5_scaffold1

the second part after the heme signature resmbles a

phthalate 4,5-dioxygenase reductase subunit

MFNKKTATTSKPDMITKCPFSNPNSLFQDLATVRQTDGIEYNEKLKGYLV

TRFDDIVDVLNRPDEFSSRPTVPDFPPPVKPLFKDKVPEKGTLLAWDNPD

HDRLRKSVASFFVPRRLKRFEPMLHDAANELIDSFIDKGTADMKSAFALP

LPLRSIVTIAGLDPERWQWIGQCLTLFGGIARRDDEEEVSIQQKIQDVLD

LHEYIRQVIEERRLDRRDDLISHIWNERDAGTVIMTDFEHLSMIPGLLLA

GHETTTNVLTMGLAHILHNGLWEQISQSDETRKAAIEELLRFESAITGMP

RQVTSDGVQLCGMKLNAGDRLFVAYNSGSRDESKFENPNKLDISRQSKTQ

HLGFGRGVHACLGAPFARLLLRTELAVLYTRLPNLRLVTPYEELAYGEVH

EARSIGATFYAWDIPSPGTARKVHVQSNGNATSRSAAVQNIPMVVQSLGS

VAQNIMQISLQPKDTAKQIEWTPGAHIDINVGTFGYRQYSVCSDPTDTQQ

LKIAVLREDQGTGGSRYLHENLKVGDKISIRGPRNNFKFTPGTRRTFLIA

GGIGITPMIPMAEEAAASGIDYKIIYLGRSRAGMAYIDKITQRHGERAMI

WVTDEHDGARLELAKLLQAEDPNGLRVYCCGPESLLTAVGESMSHAPLGT

VHVERFANPVSPLSSSNTAFDVLMAKSGRVLHVPEDKTVLEVINEAGGNV

LSTCNKGLCGTCEVGVLEGTPEHRDVVLTAAERAENSSMMTCVSRCRGKS

LVLDLW*

 

>CYP645A2 Fusarium oxysporum

60% to CYP645A1, this seq. has a C-term extension

FOXG_02323

the second part after the heme signature resmbles a

phthalate 4,5-dioxygenase reductase subunit

MTCPFSNPRNLFDDLATARETPSIEYSEKLGGYVISRYDDIVSVLDNPAAFSSRPTVPDFPPQVKQIFANKVPERGTLLA

YDNPDHDRLRKSVASFFVPRRLERFEPLLRATAHGLIDGFVEKGCVDIKSNFALTLPLRTIVIVAGLEPSRWQWIGRCLA

LFGGITQTNEELSIEQRVQDVLDLHEYVAEVIAERKTDRRDDLISHIWNERDAGVVEMTDYEHLSMIPGLLLAGHETTTN

LLSMGISHLLHHDLWNAATEDEEARSTAIEELLRYESAITGMERLVKEESKIGDHIVQPGQKLFVAYNSGSRDSTKFGNP

DKLDFKRQHKHQHLGFGRGIHACLGAPFARLLLRTELAVLKERLPNMRLETPYEEIQYCRVHSGRGPERVEIAWDVPSLD

EMRVNVGQATVNSALRSSAKTEDLEMVVENVENVTERIVTLTLRPKDGGKVPKWSPGSHVDVQAGTIGYRQYSISSKPSD

DQHIKIAVLRENDTGASNWIHQNAIKGSQMLIRGPRNHFSLEFGSRKTIFVAGGIGITPIIPMAEAAKQADVDYTILYLG

RSKNNLAFVNELTEEHGDHFKLWYWPEATRFCEYRKTNRFWR*

 

>CYP645A2P Fusarium verticillioides

85% to CYP645A2 Fusarium oxysporum

no gene model, no introns

the second part after the heme signature resmbles a

phthalate 4,5-dioxygenase reductase subunit

MTCPFSNPRNLFKDLAIARETQSIEYSEKLGGYAISRYDDIVS

VLDNPSVFSSRPTVPDFPPQVKQIFANKVPERGTLLAYDNPDHDRLRKSVASFFVPRRLE

RFEPLLRATAHDPIDGFVEKGCADIKSNFALPLPLWTIVIVAGLDPSRWQWIGRCLSLFG

GITEMNEELSIEQRV*DVLDLHEYVAQVIQERKKDRRDDLISHIWNERDAGVVEMTDYEH

LSMIPGLLLAGHETTTNLLSMGISHILHHDL*DAATKDEKLRNTTIEELLRYEPAITGME

RLVKEESKIGDHTAQPGEKLFVAYNSGSRDTTKFENTDKPNFNRQHKYQNLGFGRGIHSC

LGAPFA*LLLRTELAVLKERLPNLRPTTPYEEIQYCKVHAGRGPERVEIRWDVSSLDEMR

ANVGQANANSTLRASAKTEELEMVVKNV*KVTERIIRLMLYPKDGEKVPKWSPGSHIDIQ

AGTIGYRQYSICSKHSEAQHLKIAVLRENDTGASNWIHKNTVKGSQVLIRGPRNHFSLEF

GSGKTIFIAGGIGITPIIPMAEAAKEAGVEYSILYLGRSKNNLPVI*

 

>CYP646A1 revised Aspergillus nidulans AN2596.2 AACD01000043

this gene may have a frameshift & rather than an intron as shown below

based on comparison to other sequences

This may be a pseudogene

MLLELDVSLFSMAALVGTLVCIVVKLFETSPVPKDIPWV

LKKQNVLRRAAERFIGVNPISFIQEGYEK (0)

YSKNQQPFVMPSVNEGDEVVLPREHSNLVLFAKES

EYSFKAHISDFFQLKYTSWPLAFAEKYDFFVKLVSKDLTETLKSDAIAEA

LANEARACLSDIWGEDEDNWVEVPLYSAME

KSAA  261319 & 261317 RLINVLAIGPGNS

HDTSLLNAMTNCSNAIVFGANVIKAFPSFLHG (2)

IVGPIVGLVNRWQERVFHTRMKPLIEARIKTQREAVDKAALQENLKAQGSLLDMLINAGLRSKW

PVEVETMWLAYRIFMINFPGVHTSGISATSALLDILSFPSEEGLIDMLQAEIQNIAAS

SCGKWTAAELEQASLLDSAIKESLRFNGINALSPTRM (0)

VVAPEGTTLPNGLFLPYGTKI

GIPQYAVHRDSDLYPNPDQYNPYRFYTPGASPAQLRESSMTNTSESYVVFGHGRRQCP

GRWIFAHIFKLLIAEMLLKYEIKPFPTRPKIH (1)

RWGRFQLPPLTTKLTVRRRKDTA*

 

>CYP646A2 Aspergillus fumigatus revised 7/18/07

AAHF01000014

80% to 646A1

join(572205..572288,572338..572670,572727..573086)

locus_tag="Afu8g00960" second P450 of 8 on this accession

80% to 646A1 Aspergillus nidulans

MLLNISAFALSTTGVLCMLLCLVVKLFETSPVPKNIPWVLSKKGFFGRAAERFLGVNPIGFIQEGYQQ (0)

YSKNQQPFVMPSVNEGDEVILPKEHSNAVLFAKESEYSFKAHISDFFQLKYTSWPLAFAERYDFFVKL

VSKDLTETLKSKAVAESLAEEARSCLSEFWGEDTDNWVEVPLYSFME

KVAARMVNVMAIGPGSS

HDTTLLGAMTNCSNAIVFGANVIKAFPSFVHP (2)

IIGPIVGLVNRWQERVFHARMKPIIEAKIKEQKEADDPQSLQERLKANGSLLDLLIQAGLRSKW

PVERETMWLAYRIFMLNFPGVHTSGISATSALLDILSYPTEEGLMDMLQAEIEQIAAN

SDGSWSAADLERASLLESAIKESLRFNGINALSPTRK (0)

VVAPEGAFLPNGVFLPYGTKI

GIPQYAVHRDSDLYPNPNEYNPYRFYKENATPEERRQNLMTNTSDTYLVFGHGRRQCP

GRWLFAHIFKLLIAEMLLKYEIKPFAARPKIH (1)

RWGRFQLPPLSVKITVRRKKNPVAVCNQFST*

 

>CYP646A2 Neosartorya fischeri NRRL 181

92% to CYP646A2 = ortholog

XM_001261718

NFIA_094420

MLLDINASTLSATGVLCMLLCLIVKLFETSPVPKNIPWVLSKKG

FFGRAAERFLGVNPIGFIQQGYEQYSKNQEPFVMPSVNEGDEVILPKEHTNAVLFAKE

SEYSFKAHISDFFQLKYTSWPLAFAERYEFFVKLVSKDLTDTLKSKAVAESLAEEARS

CLSDIWGEDTDNWVEVPLYSFMEKAAARMVNVLAIGPGRSHDTSLLNAMTNCSNAIVF

GANVIKAFPSFLHPIIGPIVGLANRWQERVFHARMKPIIEAKIKEQKEAYNSHSLQER

LRTNGSLLDLLIQAGLRSKWPVERETMWLAYRIFMINFPGVHTSGISATSALLDILSY

PTEEGFMDMLQAEIEQIAANSDGSWSAADLERASLLESAIKESLRFNGVNAVSPTRKV

VAPEGAILPNGVYLPYGTKIGIPQYAVHRDSDLYPNPDEYNPYRFYKENATPEERRQN

LMTNTSDTYLVFGHGRRQCPGRWLFAHIFKLLIAEMFLKYEIKPFATRPKIHRAGWGR

FQLPPLSVKLTVRRKKNTVAVYN*

 

>CYP646A3 Aspergillus niger

fgenesh1_pg.C_scaffold_3000278|Aspni1

66% to CYP646A2

MFLSISIASWLVIGIIGVGLCLVVKLFETSSVPNGIPRVLNREDLVGRAVERFVGVNPIGFIREGYQKYSKHQKPFVLPS

VAEGDEVLLPKDLAKHVLFSKENEYSFKAYITDFFQLKYTSWPLAFAKKYDFYVKLISKDLTETLKTDSVARSLAEEARS

CLADLWGEDSETWVDISLYCAMEKMASRMINVLAIGPGHSDETLLNAMAHCSDAIVFGASIIKAFPSFLHPIIGPIVGMI

NRYFEVIFHKRMKPIIESKIKEQQKQRDPEAQEETLKSVDSLLDLLIRAGLRSKWPIEATSMWLSYRIFMINFPGVHTTA

VSATSVLLDILSDPIDENLVNHLRAEIESIAGNSSGYWNAEDLERAALLDSAVKESLRLNGINAASPTRKVVAPNGVSLA

NGLFLPCGTKVGIPQYALHRDEEIYPEADRYNPYRFYIENASESAIRQSSMTTTSDEYVVFGHGRRQCPGRFIFAHIVKV

FIAEMLLNYDVQPILVRPKIHRWGRFQLPPLTTKLTVRRKKRN*

 

>CYP646A4 Aspergillus clavatus

82% to CYP646A2, 79% to CYP646A1

ACLA_044680

MTAAMLFNLNVSTLSATALVCMLVCLIVKLFETSPVPKNIPWVLSRKGFLQRAAERFIGVNPIGFIQEGYEKYSKNKQPF

VMPSVNEGDEVILPKEFSNTVLFAKESEYSFKAHIVDFFQLKYTSWPLAFAEKYDFFVKLVSKDLTETLKSKCVATSLAD

EARSCLTDIWGEDTENWVEVPLYSTMEKAAARMINVLAIGPGESHDTGLLNAMTNCSNAIVFGANVIKAFPSFLHPIVGP

VVGLVNRYQEHAFHTRMKPIIDAKIKEQKEAYDSEKLKEHLKTKGSLLDLLIQAGLRSKWPVELDTMWLAYRMFMINFPG

VHTSGISASSALLDILSYPNDEGFGDMLQDEINKIAAGSDGSWTAAELEQATLLESAIKESLRFNGINLLSPTRKVVAPE

GATLPDGTFLPYGTKIGIPQYAIHRDSDLYPNPNEYNPYRFYKEHATADELRQNSMTNTSDTYLVFGHGRRQCPGRWVFA

HIFKLLIAEIFLKYEIKPLATRPKIHRWGRFQLPPLTVKLTIRRKKDTVAVFK*

 

>CYP647A1 Aspergillus nidulans AN8250.1 37% to 623B1 54 clan

MSTAWTNSWIAIAVTTFLVAVFVLLYSAPQSEIDIPLVRDRGRKRFRTST

FVSYLFNANDILQEAYDTYLKHGKTCRVPDKSLGTMILIPNRYMKWMLSQ

PTGVLNSYEAIAEIVQARWTLGDTRYLSDDWHLNMLRDALRPLSKSGIVE

FQEELERSMEDVLGNDHVNWKDVNPTIATKRIILQATTRYLVGSPLCADE

QFLRWILIFIEWITPVGELLRLTPRGLRSVVGHIFSIPRFIVIQKLKAIL

RPQYDKRLSFIRSGQSPEDGPQDLLQMMMRDLYERNSTDLNLHFITINVL

VFVIAAVVQTYMLAPSVLYDILESNAEYNTISKLQEELVCVLGEPAQGQR

WSQNEARQMAKLDSVLRESLRTNTLVSQTMPRKVMVDSLQTPDGITLPRG

ATVALLARAAQTDPDIYPNPNKFIPFRHAVPGGKQFAATGENFLAFGHAK

HACPGRFLVSVELKMLVAHILRHYHIELPAEYGGKRPKAVWFTDLNLPPQ

HGVIRVKRCV*

 

>CYP648A1 Aspergillus nidulans AN3256.1 poor match 54 clan

MFQAASSVLSTLYQICANGAQAPLPAGSVWAILVLLLVALILLVCRYADS

YDGVPVVNRICALEPRVFSRIRFAFNAEQILQDAYRKYDGKPYIIARGDA

DYLVLPSESVTELNRLPASIINSRMCHAYSMTGHLNGMNVVLKSNLHVKT

LLNRITPALPAFLGPASARMQATMQETFPSVSSWTTIEPLDLVVGCVSRA

ITLAAVGEPWCDDTELVNLTFEHTKLVFTVMFAMRLVPAALQPVLVWMLP

HKWRLQKSLQRLESFIVPIVQECKAAKPRPATERPSTLLAWMVAEATNDV

EEDPYVLTELLAALAAGGTYSSANFIVSVILDLIANPQFLDEIREEIRQK

HEELQGRWDFAAFNNLPKLDSAFKETIRLTPGSLTTYSRVMLQDYTLSTG

ITLKKGQFICVSSYARAKDDEIYQNAGSYDALRAYNESQQYHAAQPFKGV

YQQEFRWGAGRWACAGRHLASLLAKFIVVKLLDEYEFQFVPGSHRPPNSV

FHEFVFVHPSTRLLTRRREENLGICCW*

 

>CYP649A1 Aspergillus nidulans AN3253.1 32% to 503A1 54 clan

MEDPSSLPSLYPGLFVLFLLYFAGDLIATRRAAQRQKDHPLVGSPSWWTP

RFGLNLVFAARAVEILQTGYHKFKNRTFQLIRGDGSVVILPLHLIDELSS

LPQSVASSHGALERDLLGRYTGLDIILTSRMHHTIVQRKLTPRLAALTPS

LQDEVSLAVQEGFPHSTEWTIVKPYQILAQVAAKIAARAMVGPSFCRDPR

WLDISVNYTESLFRTIVILRLFPGWTHPVLSRCLPSYWAGKRYLQRAKGI

LGPKIDELIRRNDTGEWSPERTESDFNVLCWLVEAAKGRDRNAETLAHIE

VLLALAAVHTILLRLVNVLYDLVAHPALFEELKEEIQDIGFNEDWNFGSY

NKLRKLDSVLRESQRLSPPTILGLKRLFLQPYKFTSGITVPAGTYVALPV

MAIENDPLHTDNPEEFDGLRSYRRIEQKTASMRPNPKDGPQFSTIEKTVL

GFGYGKSACPGRYFASLVLKMVFVKLLTEYDFQFLPGRSRPKNYLVHEFL

FPWPWDKILVRRRENGVCPF*

 

>CYP650A1 Aspergillus nidulans AN9251.1 40% to  54 clan

revised 7/19/07

MIIFNISALGPPKPMLQFVGRELVETSRATMLRLPIDSAMHPFSTLPNHE

LGLYIMWMMSAVFVIFKLLAPAKCDIPTVNGRRRFEIGQYQARRRFSVDG

RGIILNGLQMARVFRVVSQKGPKIILGPEYANEVKSHPACNADVFIAKEF

HAHVSGFEVLRPQQVMKDAIRLRLTRSIGALMKPISAETALILESQWGNS

NCWHELDLNFTIA

SLVSRVSAVMFVGEELGRDQKWLSIVTNYSSDMFVADLDLCKWPEALR

PIATYFLPSCGKLRRHIREAALMLYPILSEGYSAHQNKQNFLDWLEEIAG

DRKYNPVLAQLSLAAAAIDTTSDLIIKTLTDICRFRDSQKLQEDLREEMV

RVLRADGWEKSAMYNLKLLDSVLKETQRVKPVVVFGMGRYVTEQITLHDG

TVISKGETINVVNTRIWDPAVYPNPLEWDPYRF

VRRRDSGDHAAHLVSPTPDHMGFGLGKHSCPGRFFAATKIKIILCHILLK

YDVKIPDEEISTVISSG

NFLFPDSTLRISVRRRQDNLTIWD*

 

>CYP650B1 Aspergillus nidulans AN9248.1 40% to 68a1 54 clan

MAMGNPELRTHFFRSSHEPGIPNSLRFPNGFLVTCGLPWLLLLFSVTIIL

FHPLRKKSDLPLINPGKGRIGILRGYRSRKTFTTELPRLVADGLSKASAF

RIAAPDGVNIVLAPSYAHEIAEHPDLNPGPIAGDEFNSHINGFEVFAQLG

TSDVISESVRTRLTRQLTKLTPLLTSETPLLLQSQWKDAPDWVEVSPHET

ALFILSRLSSLVFVGDDLGRNPDWIRILTSYNTEAFAAAEELNLWPQILR

PLIARLKPSCRQLRRYIRDARALLVPVLEQRRHAQSQGDRREYNDAIEWL

DETSRSTGQPYDPILSQMLLAIGSFHTSSDLLGQVLLDLCMRPDWKVLVR

ELRKEIISSLQGEGWDKIALNNLKLMDSVLKESQRLKPASTVTMGRYASR

EITLSDGTIIPKGSTVFIANVAMRDSNIYPDPDDFVPDRF

TTRREKGDSSAYLVSASPEHLGFGLGRHACPGRFFAANELKIVLSHMLMK

YDIKLPDNGA

VAPSKSGIFLATNPDARICVRRRKEEIVI*

 

>CYP651A1 Aspergillus nidulans AN9253.1 34% to 68D1 54 clan

revised 7/19/07

MLQETISLTPLGQPLIAGFVVVSAVLYLLYNTQQWRPNNLPLLNDGGPFD

FLQVTAVNRFRRDARRLIKSGFDS

MRTDVGVELFASPEYADQFRNHPSLKVFPFTAKMHHGHLPGFELCRSQPV

EDRILIESVRTQLAQSLGKLIQPLASDIGEAISDRWPSESGWQEIVLGSV

VERTIAQGTSSVYCLDEAWPEFVVKMEMALGMASAALSAWPVMLRRIVAK

FLPECLELYRIMKSGRELMSRDMRRRTALQASTGEEPLNFFEWFKEASHG

EEYDELILNLRIAFASMHGLCDHLVKILLRLSEDPQLVSDLRKEVIQVYE

THGWSKTALYHLKLMDSAFKEVQRVDPILFVGRVAVADVTLKDGLIIQKG

QSIRISGHTMWDEDKYPDAAHFDPYRFYRLRQAPGQENTAQFTSPTSDHL

GFGYGGRACPGRFFAAAVLKISLCHVLMKYDIKPANGETGQHVWEFAAAI

NANMTAKVLVRRRQPEIQI*

 

>CYP652A1 Aspergillus nidulans AN8139.1 37% to 68D1 54 clan

MLQSLLQNNFVAVLGLLIVFGTATSLTWTAFTILSPYLRVKGAKIFNDRT

GSEILWTNARKRFQRGARELFKAAFAQHPNAFYIMTDTDVELILDSKYAP

EVRNDRRFDIGKYNEDMFHGTIAGFEMFENDHVLERVFVETVRNKLTRAI

GKFVKPISLEAADGLREYWTDDTGIPAPTRNHSCVLRTIAKQSSRVFQGP

PLCYNPDWLRITVNHTVTFFEAAESLKVWPHPLRPLAAKFLPLCKKLRAE

AQEARRIITPILEERLKRAQARMAEKNNLPEKKTEVKEEGDSDGNMIEWA

EETANGAIYDAALLQMKVSLASIHTTSDLVSQTLFNLCSRPELVNDLRKE

VIEVIGQQGWVKPALYQLKLMDSVLKETQRLKPISIGTMVRTTTSPVTFS

DGLQVPANTRTLVSCHNMWTESVHENPEVFDGYRFLKLRQLPGQENWTQL

VSTSNNHLGFGHGMHACPGRFFAATTAKVLIAHVVLKYDLKLLDGQKPVI

IEHGAAQYANVWCPIGVRRRREEIDLSDL*

 

>CYP653A1 Aspergillus nidulans AN8184.1 33% to 503A1 54 clan

MSPMVLCTSLLVLLAWLLSKSPTDTTPVNVPTVTLGRFVPCFVDRLLFCA

KGPFLIYDGYKKSVCGDYTNILSDSFLPAQTVVKKLTPALGRIVPDVIDE

LKHAFDVEVPECYDKWVTVTIYSMILKLINRSTSRIIVGDSLCRSEDWLN

TVLDYTYNLGTIIFLLRPLPRFLRPLVAGLLPQVRHARKILDHVKNDIIV

PMILERRQKEANDSNYRKPDDFMQWMMDTADNDYDKQPGNIAQGLMIIMA

LAVIHTSTITITQSLYDLLIRPEYVQPLRQEIIDTLKHGWANATRADFAT

QVRLDSFLRESQRLNPTHEVNVQRIAKETLVFKDGFVVPKGTHICFAAGP

LSRDPELIDDPETFDGFRWCKDRQYNKKGHNNSLVALSKLNLHFGYGRQA

CPGRFFAENTSKAILSRLLVEYDLKFPEGKEGRRPWNIRNGEQIVPNVYT

KVLIRKRLVNL*

 

>CYP653A2  Aspergillus fumigatus Af293

          GenEMBL XP_748033.1 also EAL85995.1

          47% to 653A1 with errors, 56% to last 366 aa

          needs revision, removed intron GKYLLCVHLLECLTADESQLQ

MHLASFSLLTSITLHHYCAHNTPITLHNIDQLLNGTSLAQRRILRLSSAKLSVMADQVLELPCWPFALLL

SSSLYFFHYPPGETSAEIPTVRFSRFLPGFINRLMFIFVAPYLIKYGYEKYRDQPYRILKADGDLLVLPP

KYLPEIRHLPPSKLSGVDAQFENILGKYTNVLIDSNLPVKTVHKGLTPAI

GRVLPRLLDELQFAFQVEVPDCDDRWVPVNLYDMILGLVTRATSRVIVGKNICRNSQWLETVTSYTVNL

GVTVILLRPFPNFMRPLVAKVLPSVKKLRTQLRFVQNELFIPMILARREAEINGPAYQKPDDFLQWMMDL

AEDDFDKDPANISQMLLIVMALAVVHTSSMLMTHAIYDLMIRPEYIEPLREEIRGTLKDGWDKVTQASFA

SQRRLDSFMRESQRFNPTGELSTHRMVMETMVLSDGLVLEKGTHICFPSKPMGMDDSIIEDALTFRGFRW

CEDPEARSTSLVSISPSNMHFGYGRQACPGRFFAANTNKAILGRLIADYEFKFEGDNNKRPRNLTLGEQI

MPNMHTKILLKKRAFV

 

>CY653A2 Neosartorya fischeri

95% to CYP653A2  Aspergillus fumigatus = ortholog

NFIA_039010

MADQVLELPCWPFALLLSSSLYFYHYPPGEASAEIPTVRFSRFLPDFINRLMFIFVAPYLIKYGYEKYRDQPYRVLKADG

DLLVLPPKYLPEIRHLPPSKLSGVDAQF ENILGKYTNVLIDSHLPVKTVHKGLTPA IGRVLPRLLDELQFAFQVEVPDCD

DRWVPVNLYDMILGLVTRATSRVIVGENICRNPQWLETVTSYTVNLGVTVILLRPFPYFMRPLVAKVLPSVKKLRTQLRF

VQKELFIPMILARREAEMKDPAYQKPDDFLQWMMDLAEDDFDKDPANISQMLLIVMALAVVHTSSMLMTHAIYDLVIRPE

YIEPLREEIRETLTDGWDKVTQASFASQRRLDSFMRESQRFNPTGELSTHRMVMEKMVLSDGLVLEKGTHICFPSKPMGM

DDSIIEDALSFRGFRWCEDPEARSTSLVSISPSNMHFGYGRQACPGRFFAANTNKAVLGRLIADYEFKFEDDNNNKRPRN

LTLGEQIMPNMHTKILLKKRDLV*

 

>CYP653A3 Aspergillus clavatus

76% to CYP653A2

ACLA_002050

MIPLWPIILLFCSSVYFYFNPPGEKSNDIPTIRYSHFLPDFVNRLIFIVAAPQLIKRGYDKCKNGPYRIIKADGDLVILP

AKYLAEIRHIPPSRLSGVDAQFENILGKYTNVLVESHLPTKTVIKRLTPGI

GRVLPRILNELQYAFHVEVPDCDDKWVSV

NLYDMILNILTRATSRVIVGETVCRNPKWLQTVTNYTVNLGITVMLLRPFPEFLRPMIAKVLPSVKKLRTQLRYVQKELF

IPMILSRRYSEKNEPAYEKPDDFLQWMMDLAEDDFDKDPANLSQNLLIVMSLAVVHTSTMLMTHTLFDLIIRPEYAQSLR

EEIAATLPDGWDNATQAAFASQRRLDSFMRESQRFNPTGE LSTHRMVKEDMVLSDGLVLKKGTHICFPSKPMGMDSSIVK

DALTFDGFRWCDDPENKNASLVSIGPSNMHFGYGRQACPGRFFATNTNKAILSRLIADYEFKFEDGRKTRPNNILLGEQI

MPNMYTKVLMRKREFV

 

>CYP653B1  Aspergillus oryzae

          GenEMBL BAE61541.1

          46% to 653A1, 51% to 653A2,

MDYMDYLKEMGLAWHWVPIFVGLLAFYFIMERSTANASRTSLPIVKHFKLLPPFFNRILYVVKAPFLIYY

GYEKYKSKPFRILKLDGDLVVLPQKYLEEVGGLHSRQASLVGAKYK NILGGCTNILINSELPARTVSEKL

NPVL DRQIPRLLFELHHAFSAVVPYCEGRYVPINLYHMILKLVTHSTSRIIIGQRLCRSEQWINTITKCT

YDVRIAVKQLQLVPRFLRRLVAPFLPSVQRLETQLRWIAEQLILPMIQHRRRRELNDPSYKKQEDFLQWM

MDLADNDLDRDPMNLAYGLMITMALAVVQTSTMLITHAMYDLMVHPEYLEPLREEIHETLTNGWIRASLS

DFGAQRRLDSFLHESQRLNPPSEVSAQRVLGRPLTLSDGVTLPKGTHVCFPSGPMSRDPTVVPDPLTFDG

FRWCKDLNAPDGSLTDVSPANLHFGFGGQACHGRFFGAVIAKAVMSRLLAEYDLKFEEGQSGRPENIVNG

EQIMPSISTKVLIKKKNVDI

 

>CYP653B1  Aspergillus flavus

99% to CYP653B1  Aspergillus oryzae

AFL2G_09894 revised

MDYMDYLKEMGLARHWVPIFVGLLAFYFIMERSTANASRTSLPIVKHFKLLPPFFNRILYVVKAPFLIYYGYEKYKSKPF

RILKLDGDLVVLPQKYLEEVGGLHSRQASLVGAKYK (0)

NILGGCTNILINSELPARTVSEKLNPVL (1)

DRQIPRLL

FELHHAFSAVVPYCEGRYVPINLYHMILKLVTHSTSRIIIGQRLCRSEQWINTITKCTYDVRIAVKQLQLVPRFLRRLAA

PFLPSVQRLETQLRWIAEQLILPMIQHRRRRELNDPSYKKQEDFLQWMMDLADNDLDRDPMNLAYGLMITMALAVVQTST

MLITHAMYDLMVHPEYLEPLREEIHETLTNGWIRASLSDFGAQRRLDSFLHESQRLNPPSEVSAQRVLGRPLTLSDGVTL

PKGTHVCFPSGPMSRDPTVVPDPLTFDGFRWCKDLNAPDGSLTDVSPANLHFGFGGQACHGRFFGAVIAKAVMSRLLAEY

DLKFEEGQSGRPENIVNGEQIMPSISTKVLIKKKNVDI*

 

>CYP653C1  Aspergillus oryzae

          GenEMBL BAE65194.1, AP007171.1

          40% to 653A1 45% to 653A2, 41% to 653B1

          63% to CYP653C2 from an indole-diterpene gene cluster

          note the other P450 in this cluster is CYP698A1

          The ortholog of 698A1/paxQ (698A2) is 4 genes upstream of this gene

          14 P450 genes and 2 pseudogenes on this contig

1897240 MDKLTATLAKVNYPSEVENGSMLLVVTLVILFLWFIIPSPVKRSNVSVPTVTLFNPYLPEFLSRVWFNST

AATVIYKGYRQHKDRAFRLLKPDGDIIVLSNKYVEELRQLPLTTLNALEAVFE DHVGKYTTILNDSHLHT

EVIQKRLTPAISRFIPRIIDELDHGFAVEMPECEDKWALIRPYEVFLRLVARAGARVFVGPEICRTEKWL

TASIDFTKNIFMTITLLRPIPSFLHPIIGPMLPSSRSLDTQLRYVQDELLGPEIVKRRQRQASGDPDYEK

PDDFLQWMIDLAQNDKEGDPGNIAHRLLGLTSMAVVHTSAMSITHGLYDLITMSQWLEPLRQEIQEAMPD

WKSSSYSSLVSLRRLDSFLKESQRFNPPGELSFHRVVKKDLVFSDGLRLPKGTHICMASGPIGMDTKYVS

DPTTFDAFRYVDGDKAQSQFVHTSATSMHFGLGRYACPGRFFATFVLKAILSRFLVEYEFRFGPDQVGRP

KNMLLGDKIVPNTSVDVYVRKRTGSRSTA 1899076

 

>CYP653C1  Aspergillus flavus

98% to CYP653C1  Aspergillus oryzae

AFL2G_05469 revised

MDKLTATLAKINYPSEVENGSMLLVVTLVILFLWFIIPSPVKRSNVSVPTVTLFNPYLPEFLSRVWFNST

AATVIYKGYRQHKDRAFRLLKPDGDIIVLSNKYVEELRQLPLTTLNALEAVFE

DHVGKYTTILNDSHLHTEVIQKRLTPAISRLIPRI

IDELDHGFAVEMPECEDKWALIRPYEVFLRLVARAGARVFVGPEICRTEKWLTASIDFTKNIFMTITLLRPIPSFLHPII

GPMLPSSRSLDTQLRYVQDELLGPEVVKRRQRQASADPDYEKPDDFLQWMIDLAQNDKEGDPGNIAHRLLGLTSMAVVHT

SAMSITHGLYDLITMPQWLEPLRQEIQEVMPDWKSSSYSSLVSLRRLDSFLKESQRFNPPGELSFHRVVKKDLVLSDGLR

LPKGTHICMASGPIGMDTKYVSDPTTFDAFRYVDGDKAQSQFVHTSATSMHFGLGRYACPGRFFATFVLKAILSRFLVEY

EFRFGPDQVGRPKNMLLGDKIVPNTSVDVYVRKRTGSRSTA*

 

>CYP653C2  Penicillium paxilli

          GenEMBL AF279808

          paxP gene

          part of an indole-diterpene gene cluster, also includes CYP698A1

MDLSDFHISTPLRYFHEEASLLWKLGVFAVLVYFLLPKPTYKTN

VKVPTVKYMGPWMPEILSRIFFNSHAPTVIYKGYEKFKTSAFKVVKPDGDLVVLSTRY

AEELRQMPSTTLNALEATFTDHVGGYTTILTDSHLHTETIQKKLTPAIGRLIPRMISE

LDHAFEVEFPTCDDQFASINPYTVFLRLVARVGARIFIGDELCREEKWLQASIDYTKN

IFLTIALMRPMPGFLHPIVGRILPSSRSLKDQLSYIQQDLLGPVIKERRRLEASSDSE

YKKPDDFLQWMMDLAQNENESHPDNLSHRLLGITSMAVVHTSAMSMTHILYDLLTMPD

LIEPLRDEIRNEIKDWNKATQADLSRLIIMDSFLKESQRLNPPGDLSFHRVVKKDLTL

SDGLFLPKGTHICMAAGPISKDPDVVSDPDTFDAFRFVKQRTATSGFVSTGPNNMHFG

LGRYACPGRFFAAFVIKLILSRFLMDYDFKFETEHKERPKNLLIGDKIVPNVATPILI

KRRATKA

 

>CYP653D1 Aspergillus terreus

50% to CYP653A3 Aspergillus clavatus

ATEG_09291.1

MGAGSLYILLPVLLGYILVFYHALSISRKTKNNIPILKHSRFLPDWINRRIFFLNAPAQIQYGYDKYNNMPFLVLRPDHD

LLVLPARYLAEIRQIPSSRLNLIEAEYN (0)

NILGDYTNILKDSE

(small seq gap, 14 aa)

GRILPRTMQELQHAFDVEVPPCSDGWVSINLYTVILRLVARVASSVVFSDE

TCRNETWINTMISYSDNLGPTIVFLRPTPDFLRPLVSRLIPSVNRLKQQLRWVQEELIVPAVTARRAAQAANPDGPKPDD

FLQWMMDVAANDIDRDPRNIAQSLLIVTAVGMMHTSTMLITHALYDILSHPECFDPLRQEIKDTLPQGWINATVAAFAAQ

EKLDSVLRESLRLNPPAE (1)

VSTQRIAKEKFTFSDGFAIPDDVQICFPSGPVARDPAFLPDAHVYDAFRWCRDPEASNK

KLVSISTTNFHFGYGRLACPGRFFSANTMKAVLSRFIAEYDMRFESEHQARPANLRFGEQ

IMPSMWTKVLIRKREVKV*

 

>CYP654A1 Aspergillus nidulans AN3274.1 second half = P450 35% to 512A1 54 clan

revised 7/18/07

MILKLIPVTGGAALLLQASALLSFAWLLLAWASRRRRNRLIPGVYVAGLKSGKVPLSQARQAFIHGCA

DLMLEGYQKTQGGLFYVPSPAGERLMIPTKYLDELKN

AANEEVDFTASFSEMFEGRYTTIGQKWHLHPDVVKKSLNANLELIMPDVY

DEIVHAYRSLLTPSHDWQPVRMSEIFTQIISRASNRMLGGKALSRNRDWT

DTSINFTTDTWLASQQLKRYPAWLRPVIQHLLPEMGRVRRHFTVARQVIC

PIVQKRSESDNDTKKPLDLLQMLWEGAEPVDQTPEFMAYTALAISFAAIR

TSSSVPTHLLYDLCARPEYIAPLREEIESVLREEGSIFTKAALNKLLKLD

SFMKESQRFNPLSLLTFGRVIQSDRILHDGLVIPKGTIIGVPAHAISQDG

DFYPSPSTFSPFRFVPSAPGEKTAGFVTTNASSSLSWGYGKHACSGRFFA

ANEIKLIMAYFLLNYDFQFAGGRTERPANYTFELQNMPDETVEVLVRRRK

DGNL*

 

>CYP654A2  Aspergillus clavatus

          XM_01273842 note stop codon in genomic seq AAKD03000032.1

          ACLA_063880

1     MILELSPVTGGAALLLQASALLSLALLLLSRVSGRGRNRLIPGVYVTGLNGGKVPLSQAR  180

181   QNFIHGCADLMLEGYQKTKGGLFYVPSPAGERLMIPTKYLDELKNAANEEVDFTASFSEM  360

361   FEGKYTTIGQKWHLHPNVVKQSLNTSLGMVIPAAAAAESSDYFYATSHNRANRADYARYW  540

541   QPVRMADIFTQIISRASNRMLGGKALSRNRNWTDTSINFTTDTWLASQQLKRYPAWLRPI  720

721   LQHVLPEMSRVRRHFTVARQFICPIVQERSESEKGTIKPLDLLQMLWDGAGPADQTPEFM  900

901   AYTALAISFAAIRTSSSVPTHLLYDLCAQPEYIAPLREEIESVLCEEGSLLTKTALSKLV  1080

1081  KLDSLMKESQRFNPLSLLTFGRVIRSDRILHDGLVVPKDTIIGVPVHAISQDADFYPSPS  1260

1261  TFSPFRFVPSTPGEKTAGFVTTNASSSLSWGYGKHACSGRFFAAN  1395

      *IKLIMAYFLLNYDFQFAGDRTERPANYSFELQNMPDETVEVLVRRRKDRDL*  187174

 

>CYP654B1  Aspergillus oryzae

          GenEMBL BAE64653.1

          40% to 654A1 just upstream of CYP682B2

286826  MDLFPRDYLFAGLAVFIFWWIIDHSRHKQRYIRNVPIVGGKANLKRNRQKFVTNSLQILRTGYEE  (0) 287020

287082  NNDELFYVPTPIGERLIIPGRYLEELKSADMAVVDFQATFLE (0)  287207

MFEGAYTTLGTHSRLLPQVVRAQLNQYLPDVLPEIQFKVALQIHREIPFNAITECFAKSDWTVINVTELM

AVLVARVSSRMFGGPALSQNREWIEASLRFAHDGFNAAQKLKMWPDTLKFIGQHFIPEVRSIKNTYKIAE

RAIIPLLDEREVDKSKKAHDLLTWMYDQAQGAEKDKKFIAGTLLKVSFAAYHTSAAAPTQLLFDIAAMPE

HIAPLLGEYLSAPRDNNQNISVKGFAQMVKLDSIMKESQRFNPLLLLTFERIIKRDFTLSDGVVIPANTW

IGCAAQAIGMDRKLYPDPDTFDAFRFVAKEEATATSTSVPATKAHYTSANPGSMAFGYGQHACPGRFFAM

MEIKAIIGEILSRFEMRLADGEMRPPSVTFETQHLPHPAGKVLFKRRRCT

 

>CYP654B1  Aspergillus flavus NRRL3357

          GenEMBL AAIH01000140.1

          99% to CYP654B1 Aspergillus oryzae

          AFL2G_04897

      MDLFPRDYLFAGLAVFIFWWIIDHSRHKQRYIRNVPIVGGKANLK

      RNRQEFVTNSLQILRTGYEE (0)

      NNGELFYVPTPIGERLIIPGRYLEELKSADMAVVDFQATFLE (0)

4806  MFEGAYTTLGTHSRLLPQVVRAQLNQYL (2)

      PDVLPEIQ (1)

      FKVALQIHREIPFNAITECFAKSDWTVINVTELMAVLVARVSSRMFGGPAL  5162

5163  SQNREWIEASLRFAHDGFNAAQKLKMWPDTLKFIGQHFIPEVRSIKNTYKLAERAIIPLL  5342

5343  DEREVDKSKKAHDLLTWMYDQAQGAEKDKKFIAGTLLKVSFAAYHTSAAAPTQLLFDIAA  5522

5523  MPEHIAPLLEEYLSAPRDNNQNISVKGFAQMVKLDSIMKESQRFNPLLL (1) 5669

5722  LTFERIIKRDFTLSDGVVIPANTWIGCAAQAIGMDRKLYPDPDTFDAFRFVAKEEATAT  5898

5899  STSVPATKAHYTSANPGSMAFGYGQHACPGRFFAMMEIKAIIGEILSRFEMRLADGEMRP  6078

6079  PSVTFETQHLPHPAGKVLFKRRRRT  6153

 

>CYP655A1 Aspergillus nidulans AN8411.1 33% to 52F1 52 clan

aspyridone biosynthesis

Nature Chemical Biology 3, 213-217 (2007)

doi:10.1038/nchembio869

Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans. Sebastian Bergmann1, Julia Sch�mann1, Kirstin Scherlach1, Corinna Lange1, Axel A Brakhage1,2 and Christian Hertweck1,2

MSLASTLPRASFETLLQHTNFMDGIKFVFFAFVVYSCFTIAVGWVVYEWK

RKAHGCGKIPRYPHRDPFFGFDIVFGMAKSLRNDYFLVWLNKVHENLPKT

FLVNFVGTRFIYTIEPENMKSMSAINWQDFAVGPMRRNNKATAPFADKGV

NTVDGHEWEFSRFLIKPFFKRETFTDTSRLTLHVDRVLEQLPADGETVNI

QPLIQRWFLDVTTASLFGESIESLVYPERAPICWAMVDVLRGLRLRLQWY

KYLWLFRHQAWLDAVEVVHRYLNAHIDRTYKELDEYKRQGKNPEAADRND

LLWYMASNLQDKEALRSQICLIFVPNNDTTSIFISHILWNLARHPGIYEK

CRQEVLALGDAELSFSVLRNMKYLNAVLNETHRLFPNGVTQVRKCIRDTT

LPVGGGPDGKQPIFVRKGDVVQVNKNVIHRDHDIWGPDAEDFRPERWENL

RPYWNFVPFGGGPRRCPAQMLVTAEASYFLARLMRVYKRIEARDPNPYVG

VMRVGPSNKTGVHIALFKE*

 

>CYP655A2P  Aspergillus oryzae

           GenEMBL BAE65179.1, AP007171.1

           pseudogene

           60% to 655A1 partial C-term seq

           14 P450 genes and 2 pseudogenes on this contig

1863432 QGDVVQVNNNVMHRD

KDVWGEDADEFKSERWFGLRPYWDFVPHSGGPRRYPAQLLVTTEASYVVARFCQRSKAVENRDVNGYIPI

MRAGPVDSNGVKIAVTPV* 1863121

 

>CYP655A2P  Aspergillus flavus

Supercontig 5: 1921861-1922169 -

95% to CYP655A2P  Aspergillus oryzae

QSDVVQVNKNVMHRDKDVWGEDADEFKSERWFGLRPYWDFVPHSGGPRHYPAQLLVTTEA

NYVVARFCQRSKAVENRDVNGYVPIMRAGPVDSNGVKIAVTPV

 

>CYP655A3  Neosartorya fischeri NRRL 181

          DS027697

          join(287915..288538,288596..289115,289183..289287,

          289332..289600)

          88% to CYP655A1

          adjacent to a PKS similar to FUSS polyketide synthase FusA

          FusA is used in Fusarin C biosynthesis

          NFIA_001540

          Note: this seq does not have an ortholog in A. fumigatus

MMSLATTLSKESLQTLLQHATFLDGIKFVFSAFVIYSCCIIAVD

WIVYEWKRKAHGCGKIPRYPHRDPFFGFDVVLGMAKALRNDYFLVWLNKVHENLPKTF

LVNFVGTRFIYTIEPENMKSMSAINWQDFAVGPMRRNNKATAPFADKGVNTVDGHEWE

FSRFLIKPFFKRETFTDTTRLAIHVDRIFGLLPADGETVNIQPLIQRWFLDVTTESLF

GESIESLVYPERAPICWAMVDVLRGLRLRLQWYKYIWLFRHQAWLDAVDVVHKYLNAH

IDRTYKELDEYKRQGKDPETADRKDLLWYIASNLQDKDALRSQLCLIFVPNNDTTSIF

ISHILWNLARHPEIYEKCRQEVLAVGDAELTFSVLRNMKYLIAILNETHRLFPNGVTQ

VRKCIRDTTLPVGGGPDGKQPIFVHRDIWGPDAEEFRPERWENLRPYWNFVPFGGGPR

RCPAQMLVTAEASYFLARLMRVYKRIEARDSNPYVGVMRVGPSNKTGVQIALFKD

 

>CYP655A4 Uncinocarpus reesii

58% TO CYP655A1   Aspergillus nidulans

UREG_06496.1

MGFTRWVFELPDVTFDMVPKWSYLFYFIFFFVITKSATSSISRSLAERRKGYGSVPRYPQLIPIMGFDIAFSMAKSLRNH

TFLLWLRMLHDTIPGKAKTFSIDFLGRHMIHTIEPENMKALSATVWKDFGVEPLRRSTGASMPFADKGVNTTDGHDWAFS

RFLIKPYFLREAFSNTDRLKVHTDNLLSLIPQDGSTFDMQTLMQRWFLDTSTNFLFGESMGCLLYPERAEIAWAMTDILR

GLRLRLQMSKWLWLFRWKVWFSAIDVVHDFIDRQVDRAYSERADAAKGQKQSSFGVELKPERTDLLWSMVGNVPEDRERL

RSEMLLLFVPNNDTTSIFISNVFWNLARYPEVYAKVREEVLSIGENAPLTYEALRAMKYLDAVLNETHRLYPNGIMQVRY

CIKDTTLPLGGGPDGQSPIYVRKGDVVQVNKNVMHRDKDVWGEDADEFRPERWFGLRPYWNFVPFGGGPRRCPAQLLVTT

EASYVVARFCRRFKAIENRDSKGYVPVMRAGPVNTNGVKIAVTPV

 

>CYP655B1  Aspergillus oryzae

          GenEMBL BAE56825.1, AP007154.1

          49% to 655A1 C-term in gene model is wrong, revised

MNNAISEPRITPSLRPSPKMQAPTLTSASAEGSDTTAHGCGGCTHFACQPIIMFTLTEYFIAACVWLVLY

KVGNLLWNRHH

YFKQQKARGCGEIKHYRHRDPILGLDFVYTLSKAFKEHRWLPWQQELFAAQGVKTFQAN

FLGSRAIYTSESENMKAMSTTYWREFGLEPLRRGSGAADPVAGPGVSTVDGPMWDFSRNIIKPYFTRDGY

SNLARLEVFVNRLLDLVPTDGSTFDMQPLLQRWFLDTSSEFLFGKTVDSLTHPENVKVAKAMVDTMRGIR

VRLTMSKLMFLHRDPVWMENVKIVRDFVDERIDASLTQLQDVKSGKGTSCTENQPDGRTDLLWDMVQQLQ

DKEALRGQIMAVFIPSNDTTSILISNAIYALARHPHVYQTLREEVLALGDQEITFEKLRG

717492 LRYLRYVINE (1)

717406 THRLYPNGIQMVRIALEDTTLPVGGGPDQSQPIF

717304 IQKGDIVHANRYLMHRDPDNWGPDAEVFRPERWGDVRPLWKFVPFGGGPRICPAHVLVDT  717125

717124 EASYVLLRFVQRFRTLEPRDERPYKAIMRIGPSNLHGVNVAVKTA*  716987

 

>CYP655B1  Aspergillus flavus

100% to CYP655B1  Aspergillus oryzae

AFL2G_07514

MFTLTEYFIAACVWLVLYKVGNLLWNRHHYFKQQKARGCGEIKHYRHRDPILGLDFVYTLSKAFKEHRWLPWQQELFAAQ

GVKTFQANFLGSRAIYTSESENMKAMSTTYWREFGLEPLRRGSGAADPVAGPGVSTVDGPMWDFSRNIIKPYFTRDGYSN

LARLEVFVNRLLDLVPTDGSTFDMQPLLQRWFLDTSSEFLFGKTVDSLTHPENVKVAKAMVDTMRGIRVRLTMSKLMFLH

RDPVWMENVKIVRDFVDERIDASLTQLQDVKSGKGTSCTENQPDGRTDLLWDMVQQLQDKEALRGQIMAVFIPSNDTTSI

LISNAIYALARHPHVYQTLREEVLALGDQEITFEKLRGLRYLRYVINETHRLYPNGIQMVRIALEDTTLPVGGGPDQSQP

IFIQKGDIVHANRYLMHRDPDNWGPDAEVFRPERWGDVRPLWKFVPFGGGPRICPAHVLVDTEASYVLLRFVQRFRTLEP

RDERPYKAIMRIGPSNLHGVNVAVKTA*

 

>CYP655B2 Fusarium oxysporum

59% to CYP655B1

FOXG_14589

MSSRLSAISLAAFYGLVILYFLNRLWDHISYSIKTKKYKCGPLKTYPHWDPIWGIDFVLSMSRAFKEHRWLSWMEETWSA

QGTKTFKARFLGMRMVYSSEMENMKAMSTSQWEEFVLEPIRVDNGVATPFTGKGVSTADGEFWHYSRGIIKPYFERQAFA

NVARLKPFTDKMLDLIPTNGDTFDMQVLTRRWFLDTSTEFLFGKSRDCLTYPEREDVMLAMVDIMRGARVRLTMSKFMFL

HRDPKWYESIRFVHDFMNEYIDQAYDELHLRKEQGEKFADKPERTDLLWDMVQKIPSEDRILLRDQITAVWVPSNETTSI

HISNAIYQLARHPDAWEKLQKEVLDLGDEELTFSKLRGMKYMNWVINETHRTIPNGIQMIRVAAHDTTLPRGGGPDGKQP

IFCAKGDIVHCNRYLMHRDPDYWGKDAAEFRPERWDGLRPLWHFVPFGGGPRICPAHILVATETAYVLTRFCQKFKGIEA

RDERVYVPVMRVGPSSLNGIKIAVTPR*

 

>CYP655B3P Neosartorya fischeri

51% to CYP655B2

NFIA_045730, NFIA_045740 join

Note: this seq does not have an ortholog in A. fumigatus

MFTAPLSIAILSITLLYWVVRVYLNYLRYERTAAQFGCPPLKHYPGWDRILGLDYVYAMFKALKEDRFLEFQTETYSARG

SKVWTANFMGNRMVYSSEPENMKAMSTLQRDCFAVEPIRVANGAITPFTGRGVSSSD GAKWQSSRDLVKPYFKHEGFSNLERLSQHVDRLLSKIPTDGSTVDMQPLFQQ* (0)

FLDTSTDFLF*ETVNSLDNPERDWPHRDMVTVMRGLRLRLQLSSFLFLHRDKEWFATCKR

IHGFLDGYIDKAYKHLEYEKSGKPATYANGEP*DDFLWTIARQVPDKLELRTQLTSIW

IPSNETTSILMSNTLFALARHPEVVEKLRKEILDYGDKPLTFEGLRFMVYLRWTINE (1)

SHRLYPVSLQTVRSCVKNTTLPTGGGDDGKAPIFCAKGDIVHCNWYLMHRDPDLW &

GADAEQFRPDRWEKVRPMWNFVPFGGGPRICPAHIMVDTECSYTIFRILHHFKAIEARDSEPYTAIMRV &

SPSNKNGCRVAFIPA*

 

>CYP655C1 Beauveria bassiana

AM409327

Chembiochem. 2007 Jan 11;8(3):289-297

Biosynthesis of the 2-Pyridone Tenellin in the Insect Pathogenic Fungus Beauveria bassiana. Eley KL, Halo LM, Song Z, Powles H, Cox RJ, Bailey AM, Lazarus CM, Simpson TJ.

Gene name tens1, 52% identical to CYP655A1

44% to 655B1

MLPLLDSVSLPYLILSACLSVILLRRFLAHDKGGSKSIAQGCLP

EPRLRQWDPIFGFGIVISQARALRGHRYLEWLRDLHASMPHTKTFSANYGGYRWIFSI

EPEILKAVYATNLQNFGVEPIRQHPPGFQPFAHKGVSTSDGDDWSFSRTLIKPFFERS

VYISTDRIKPFADKFMTLLPDDGETFDIQPLLQRWFLDITSEFIFGKSQDSMTHADRA

EVTWAMADVLRGGRQRAQTHRILWAFNWDWWFEAVEKVHGFLNPYIRSTLKELEERQQ

RIKDGLPVDEERTDLLWSMATMLPDEEELRSQVCLIFVPNNDTTSMFIGHCLYFLARN

SNAWKRLRDEVDAVGDAPITFEMLRNMKYLNGILNETHRLIPNNVTQVRAALSDVVLP

LGGGPNGKAPLDVRKGDIVSVTKTVMYRDPEQWGPDANEYRPERWDGMRGGWHFLPYG

GGPRRCPAQMMVQNESAYMLFRLAQKYSTIVARDPEPFRARMRIGPSSMHGVKIAFYK

 

>CYP655C2 Fusarium oxysporum

55% to CYP6455C1

FOXG_14594

MALHQLLAQQVTTRNLPILAVGAAFLIFIFRKLFTSSQKSEAKGWKPIPRYWQWDPIWGLDLVASQIHALRSNQFLAWLS

KLHASMPHTKTFSINMFGVKWIYTYDPEVLKAVYATHFKEFGVTPIGNLRRGLTPFADKGAITTDGEDWKHSRFLIQPFF

DRQVYTSTDRVARHTDQFIKLLPDNGETVDLQPLLQRWFLDIATEFIFGESTEALVYPERAKIANTMLDVLDGGRLRAQL

NRLMFLRDWTPWLKSVEEIHAFVTPPIQETIKEKHEREKKTKEGATDLEPERTDLLWTMVQNLEDVEELRSQICLILVAN

IDTTSVFISNCVWWLARYPDVWDKIRQEVHELGDIPMNFSVLRNMKYLNCVMNETHRMIPNNVSQLRGAFEDVVIPVGGG

PDGKSPTLIRKGDLVLINKTVMYRDPEAWGEDSNEFKPERFDGYRGSWGFLPFGGGPRRCPAQMMVQTEAAYILAHLAKT

FKRIEPRDPEPYVPVMRIGPSNKTGVKVALYR*

 

>CYP655D1 Fusarium oxysporum

43% to CYP655C1 Beauveria bassiana

FOXG_03951

MSNSTGFIADLPGLFTDIPGYVADIPSRIDFTSIPVILSSTVAVLAFTSFVKYVAERQQEKAMGFGHVPTLWQLDPFYGF

DAFVAMLLALKNNRYVPWLDSLHAKMPKTFALTFLGGRQIWTIEPENLKTVANNLADFQVGPIRYNSKAGQPFAEKGIVG

ADGKDWEFARALVKPFFMREWYANTERMEPFAERFFAGIPADGETFDMFPHLRTWSLNLNLQFLFGDYSDIVTEEETNNT

TDAFITGLAYARIRLLLHRVLWLFPWKTWYKVVGTIHSYVNKHIAKARAELKEREERISKGLPVGPERRDVCWHMVRELP

NDDEVRSHLCLLFVPNNETTLIFLSNVMWNLARQPEIWERLRQEVKGKELNFNSLRGMKLLQNVLTEAHRITPVGLFIAR

AAVRDTTLPRGGGPKGDQPVRVRKGNIVQINKAVMYKDPDYWGDDALEFNPDRFDKMRPGLSLTPYNTGPRRCPGEMMTM

NECGYMVARMAQTYKRLECRDPTPFVGAFRIGQLNKNGCQVAVYRE*

 

>CYP655D1 Fusarium verticillioides

96% to CYP655D1 Fusarium oxysporum = ortholog

FVEG_11928

MANSTGFMADLPGLFTDIPGFVADIPSRVDFTSILVILASTVAILAFT

SFIKYVAERQQEKAMGFGHVPTLWQWDPFYGVDAFIAMLLALKNNRYVPWLD

SLHAKMPKTFALTFLGGRQIWTIEPENLKTVANNLADFQVGPIRYNSKAGQP

FAEKGIVGADGKDWEFARALVKPFFMREWYANTERMEPFAERFFAGIP

ADGETFDMFPHLRTWSLNLNLQFLFGDYSDIVTEEETNKTTDAFITGLA

YARIRLLLHRVLWLFPWKTWYKVVGTIHSYVNKHIAKARAELKEREERISK

GLPVGPERRDVCWHMVRELPNDDEVRSHLCLLFVPNNETTLIFLSNVMWNL

ARQPEIWERLRQEVKGKELNFNSLRSMKLLQNVLTEAHRITPVGLFIAR

AAVRDTTLPRGGGPKGDQPVRVRKGNIVQINKAIMYRDPDYWGDDALEFNP

DRFNKMRPGLSLTPYNTGPRRCPGEMMTMNECGYMVARMAQMYKRLDCR

DPTPFVGVFRIGQLNKNGCQVAVYRE*

 

>CYP656A1 Aspergillus nidulans AN6321.1 related to CYP52 52 clan

MSAVLLGKRYVSTPDPENIKAVLATKFVDFDLGERNAAFRPLLETGVFTQ

DGREWERSRALLRPIFNRAQALPDLLLIEEHVQSRLYRIPRGYDIVNLQQ

LVRDLTLDSAFIHFLA (1)

GRPAKIAGSRRGDFDAEPFSHAFDEARSYLQVRANLGPFRGLVKNTKFVD

DVIVHAAVDEFVYEALEQRRSPAHENRGKLGRGCGN

ESESEGWYDLLSKVADTVSDGVQIRSQLLHVLLAARDT

TARLLSSVFYMLARHPSVWRKLEREVLVEFGLATQGDGKCPLPTYTQLRE

MKYVRAVLNE

ALRLLPPVPTNIRCATQHTFLPRGGGVDGCQPIFVAKKAIVHYS (?)

AEQFRPERW (2)

VPLRRGW

GFLPFSGGPRICLGQQKALTEAVYVVVRMVQTFCDIEARDERPWREQMG

LVLSSYYGVKVGLKSRNGGS*

 

>CYP657A1 Aspergillus nidulans AN5137.1 similar to CYP8 27% to 641A1 cyp7 clan

revised 7/18/07

MNTIQGAYDGLQPKTLVLAAIYILICV

IIFTRILTGLQSYKKTDTAQPRRPRTAPYWIPWFGHSLSFARNHIEFLEN

TRHRLNETVFAIVMSGAKHNVVMSPSMIKSVLTFRGVTTAPLVQHVSRNI

LGDRGVFQKLNPSDRHVFVHNVPNQFMHEPSLSQTSGAAARFIERETPNL

VTFSAAPIDQMLWERPGDVTVIEGKGQQVCEVDFFALIRYFVGTVTTTSL

FGQAILDTFPTLLQDVWSVDDQFATLSMGPPRYLTPGISAAYMARDRLLD

ALAIFHQALLLWDEGKDLGMEFRDLRDLEDVSEPIKNRARMAKDMGLTPQ

ESAPAHLALLWAMNGNSPNIVFYHLLHLYANPTLLEDLRKEIAPFVKVSR

PTREETGFPILEAPRLSIDIDKLCDSCELLKASFYETLRLDSAGLSFRQL

TADLTITESEEEASKAGRLTPESYSLKNGELVIIPHGVIHNDPTHFSNPD

QFDPLRFIRTDPQSGQKYAKSETMTPFGGGMPACKGRAFAEKKILALSAA

IISLWQITPAEGKKFKIPEHRISSAAFLPKNDIRVRMSPRYPS*

 

>CYP657B1  Aspergillus fumigatus Af293

          XP_750481.1 also EAL88443.1

          51% to 657A1

MDDVREWIWPILLAILLSSCLATRIITGLKSWPDKRIDARLPRSVRVVPYWLPWLGHSIPFGWDHIDFVR

RARDY MNEAVFGIILGGLKHDVVVSPSLTKAILASRGTSSNALINYALEKVGGDQGAIRSLSATDHHIIH

HNIPNLLMREPFLTEASKVAKDITEREAPNLVTFCRSMVDQAPWERRSEAEVTDGQEKLMAEVNLFALVR

NFVGHISTSVLMGQAFLEAFPDLLDDMWTFDNRFPVMALGAPRWLPLPGLSAAYAARDRILDALAAYHQA

FVSWDEGNDPGVKFRDLDDVSESMKQRIRKFKELGLSPRSSAPGHLSLFWALNANSPNVVFWNILRIYSD

PALLEEVRKEIAPFVKVYRPSREETGLPFQEPVKVSLDPDELFRSCPLLKASFYETMRLDSAGLSFREVT

SDLTVTESPEDAAAASLAEPRTYRIKKGGNIVMAHGMVQRDPRLFSNPEQFDPLRFVVTDPDTGARKADM

HTIYPFGAGVSGCKGRALAERMNLLFTAAIISTWDIEPASGKALTVPGHRPSSGAYLPKDDIRVRLRMRV

 

>CYP657B1 Neosartorya fischeri

94% to CYP657B1  Aspergillus fumigatus = ortholog

NFIA_017390 revised

MDDVREWIWPTLLAILLSSCLATRIITGLKSWPDKRTDAQLPRSVRVVPYWLPWLGHSIPFGWDHIDFVRRAR (2)

DYMNEAVFGIILGGSKHDVVVSPSLTKAILASRSTSSTALINYALEKVGGDQGAIRNLSATDHHIIHHNIPNLLMREPFLTE

ASKVAKDITERETPNLVTFCRSMVDQAPWERRSEAEVIDEQEKPMAEVNLFALVRNFVGHISTSVSMGQAFLEAFPDLLD

DMWTFDNRFPALSLGAPRWLPLPGLSAAYAARDRILDALAAYHQAFVSWDEGNDPGVKFRDLDDVSEPMKQRIRKFKELG

LSPRSSAPGHLSLFWALNANSPNAVFWNILRIYSDPALLEEIRKEIAPFAKVHRPSREETGLPFQEPAKVSLDPDELFRS

CPLLKASFYETMRLDSAGLSFREVTSDLTVTESPEDAAAASLAEPRTYRIKKGGNIIMAHGVVQRDPQLFSNPEQFDPLR

FVVTDPDTGAQKADMHTIYPFGGGVSGCKGRALAERMILLFSAAIISTWDIEPASGKAFIIPGHRPSSGAYLPKNDIRVR

LRMRV*

 

>CYP657B2  Aspergillus oryzae

          GenEMBL BAE61082.1

          52% to 657A1, 60% to 657B1

MGKDGLSTDGLIQNAWSGSSELVWPLLATVLVLCCIATRIISGFQSRVDSKTEQPQSVKTLPYWFPWLGH

SLSFVWDH VSFTEKSRDYMNASVFGSYMGGTK HNTIVSPSMIKSIMQSKATTSAPLVNRALKAFGDDLGS

LRDLNTTDYQVFHHKLPNLLMREPFITEASETTIQLLKREVPNFVTFCRSVVDQTLWERGSDVEVVDDNG

DKPACEANLFDLVRGFIGNITTTTLMGQAILEAFPSLLDDLWVLDNRFPLLAIGVPRWAPLPGVPAAYAA

RDRILNSLAAYQQAFLQWDDGIDPGVKFRDLEDVSEPLKQRIRTSKNLGLSPRSSAPGHLSLLWAMNVNS

SNIAFWYLVRLYNDPTLLEEIRKEISPYVKAHRPSREETGFPFEEPPRISLNLKGLLDSCPLLKASFYET

LRLDSADMSFRKLTSDLTITESNEDVTNSDRTKPHSYKVYKGESLILHHGVLQNDSRCFSNPSQFDPLRF

IITDPETGEKKANMHTIHPFGGGMSGCKGRTFAERQLLAFTAAMIVMWDIEPMDGSHFTVPRHRQSSGAY

LPKNDIRGKTLWNVNNHGVVVFIRGGRHYLAPPMADRDLSFCITCRIYIVQNIHI

 

>CYP657B2  Aspergillus flavus

99% to CYP657B2  Aspergillus oryzae

AFL2G_03848

MGKDGLSTDGLIQNAWSGSSELVWPLLATVLVLCCIATRIISGFQSRVD

SKTEQPQSVKTLPYWFPWLGHSLSFVWDHVSFTEKSR (2)

DYMNASVFGSYMGGTK

HNTIVSPSMIKSIMQSKATTSAPLVNRALKAFGDDLGSLRDLNTTDYQVFHHKLPNLLMREPFITEASETTIQLLKREVPNF

VTFCRSVVDQTLWERGSDVEVVDDNGDKPACEANLFDLVRGFIGNITTTTLMGQAILEAFPSLLDDLWVLDNRFPLLAIG

VPRWAPLPGVPAAYAARDRILNSLAAYQQAFLQWDDGIDPGVKFRDLEDVSEPLKQRIRTSKNLGLSPRSSAPGHLSLLW

AMNVNSSNIAFWYLVRLYNDPTLLEEIRKEISPYVKAHRPSREETGFPFEEPPRISLNLKGLLDSCPLLKASFYETLRLD

SADMSFRKLTSDLTITESNEDVTNSDRTKPHSYKVYKGESLILHHGVLQNDSRYFSNPSQFDPLRFIITDPETGEKKANM

HTIHPFGGGMSGCKGRTFAERQLLAFTAAMIVMWDIEPMDGSHFTVPRHRQSSGAYLPKNDIRGKTLWNVNNHGVVQNIHI*

 

>CYP657B3 Aspergillus clavatus

72% to CYP657B1

ACLA_027250

MGDPGVFSSSLLQSLLSNDPRELIWPTLLAIIVFSCLATRIITGFQSWPEKGAGAGLPRLIRTVPYWVPWLGHGISFGWD

HVVTHQESRNHMDEAVFAIIMGGTRHNIVVSPSLARAILSSRSTSSVSLINYALEKIGGDKGAIRGLSSSDHHLIHHNNP

NLFMREPFLTDASKDATSLIERETPNLVTFCRSMVDQAPWERGSEAEVIDGHDKPISTVNLFALVRNFVGHITTSVFMGQ

ALLEAFPDLLDDIWVFDSHFLALSLGAPRWLPLPGVSAAYAARDRILENLAAFHQAFVSWDDGNEPAVEFRDLEDVSEPV

KQRIRKFKELGLSAKSSAPGHLSLLWAMNANSPNAVFWHILRIYSDPALLEEVRKEIAPYVKVSRPSREETGFPFLEPPK

ISLDSDGLFKSCVLLKASFYETMRLDSASLSFREVTTDLTVTESLEDAAAAGLTQPRTYAIKKGENVSVVHGAVQRDPRF

FSNPDQFDPLRFVITDPETGEKRAEMHTISPFGGGVSGCKGRAFAERKILLFSAAIISMWDIEPVEGSEFKIPRHRRSTG

QFAPKNDIRVRMSTRV*

 

>CYP657B4 Aspergillus terreus

61% to 657B2

ATEG_10169.1

MLSALLYVYLPPGAMGEYEITKDVLIQTARNNLTALGWTALCAAALICIITRVITGLQSRTDGSSKTSDVRPVRTVAYWI

PWLGHGISFVWDHISLIEKARDSLKEPALGIYMGGTKHNVVVSPSLVKAVMLSPSTSSVAWINYALKTVGGDRGPIRNFN

AADHHVFHHDLPNLFMREPFITEASTKLVELLKREIANFVTFSWSPVDQAPWERSSDAVLTDRSDKPVCEADFFALIRGF

VGHITTTTLMGRAILEAFPRLLDDVWELDNHFPLMAMGLPRWLPFSGLPAAYAARDRILEVLAAYQKAFLDMENGIDGDV

KLRDLDDASEPVKQRIRMSKNMGLSPHDSAPGHLLLLCAMNGDSPNVAFWHLLRLYTNPALLDDIRQEIAPYVKAWRPSR

EETGFPIPEAPKVSMDFEAMLKNCHLLKASFYETMRMDSAGLSFRELTSDLTITESKDDAAVAGVEEPRSFRVNKGESIA

VPHGVLHNDPRYFSNPGQFDPLRFLVTDPETGAKRAELHTIDPFGEGASGCKGRVLAEREILAFVAAIISMWNIEPADGK

EFKLPKHQPSRGAYLPKNDIRVRLSMRV*

 

>CYP657B5 Aspergillus niger

estExt_GeneWisePlus.C_50091|Aspni1

Supercontig 5: 118325-119770 (+) strand

60% to CYP657B1, no introns

note name typo: called CYP675B2

WPAIVAILVSICVVTRLITEFQSRPRGISEGRPRPVRTVPYWLPWLGHGPA

FAWDHVDLLQKSRFSMKEAVFGIYMGGTKHDIVVSPSMVNSVLTARGTSSTDLINYALEKIFGDRGVIRNLNPVDHHTLN

HNIPNLLMREPFLTEGTRNATRLIEREIPNLVTNCRSIVDQMLWERGSQAIVVDGEDSLACEVNFFALIRRFVGHVTTAI

FMGEAILEAFPALLEDVFTLDNQFVLLSMGIPRLAHPGVSAAHVARRRLLDSLTAYHQAFVSWDDGIDPGLTFRDLDDIS

EPIKERIRKCKELGLSARSSAPAHLSLLWAMNGNSPNVVFWHLLRMYASPTLLEEIRKEVAPYVKIVRQESGFAFLEAPK

LSIDTEALVKSCPLLKASFYETLRLHSAPLSFRGVTADLTITESAEDAAIAGVAQPRSYELKRGDTVAIPHAVLHTDPKY

FANPRDFDPMRFISTDSETGQRKANVHTIKPFGGGVSGCKGRAFAERKLLAFAAAIITMWDVKPAQEGGFTVPEHRPSGA

AYLPRKDVRVRITQRA*

 

>CYP657C1P Uncinocarpus reesii

40% to CYP657B1

model is missing the heme signature

UREG_03228.1

MENAFGARRSTRAFNRASFHADSGPIQFLSKDPWLTDVSSSLVREVQKAMPNLLSFSPSVV DQSTWERLSDVYYKQEDGG

PVCEVGLFSLVRNFVGMISTTVIMGTAFTETFPYALNELWAFDSRFNAVLLGIPRWVPFPGLVTAYAARRRLLLALKSFH

NGLAASETGVDAGFDWRDMDDVSEVIKARSRALTDAGYTAEQAACEHLAFFWAMNMTTNTMIFWNLVHILLDKQLKQEIM

EEIKPHSKVTRSDWGESGFSIPEPPRLHIDAAALVSACPLLKATYYESIRVYATPLSYRQLINDITLSEPTATTTVATSR

PNTYKCDAGSYVAVPHFMHNMDPKSFPDPEDFRPWRFL

(heme region is missing )

PEKSEETSDVSDEDLSLEDEKLIADD

ESISQKMRDVWPREGSTILSYSPE

FAERQALIFTAAFLTMWDIEQADGKAWKVPRPTDGSATCVPRKDIKVRMKLRV

 

>CYP657C2 Coccidioides immitis

66% TO CYP657C1P, HEME SIGNATURE IS MISSING

note C is found adjacent to the AG pair in the I-helix

This may sub for a Thr that points to the heme.  Cys on the other side of the heme?

CIMG_02482.2

BOTTOM PART IS SEPB, A CHROMOSOME SEGREGATION PROTEIN, NOT P450

MAAALLKQSSSLSTEPATDYILKNAFGAGRSVGTLNRSDFYGDSGPIQ

FLNKEPWLTDITSAIARQVQQAMPNLLSFSPSVVDQSTWERVSDVSISHENGEPICEVYLFALVRNFIGTISTTALMGSA

FTETFPYALNELWNFDGNFNAILSSIPRWIPFPGLVSSYSSRRRLLLAMKVFHDAFAATEIGVDPGFDWRDMDDVSEVVK

ARSRALIEAGCSAEAAASEHLAFFWAMNTITNTLVFWNLVHILSDEELHEKILEEIAPYSNAARPDWRESGFNIPEPPRL

RLDANALVSSCPILKATYYESLRLYSLPLSYGQVTKDVSLAEPTVVDSPSSTYKCEAGTYVAIPHFIHNMNTDSFHEPTK

FDPQRIFPHDLEKD

(heme region is missing )

SDTPDNYESSENEKLISN

DSISQKVNDIWPSEASPVLGFSRE

FAEGQALVFTAAFLTMWDIQRASGRRWTVPRQKAGSAVCVPRNDVMVRMKLRV*

 

>CYP657D1 Histoplasma capsulatum G217B

43% to CYP657C1P

ABBT01000229.1 Ajellomyces capsulatus G217B

HCB07501.1

176305  MERVFGAQENTRNLTREDFDAQCRLMEFQMREPFALNCSSIALRQIEQNIPNMLSFCSSIV

DQSLWERMSAVSLVSDGNGQACEVNLYTLVRNFAAVTVMSAIMGEDFMEALPNTPD

DIRTFNNNFNAIVLGIQRWVPFPGLPTSYRSRRRLLQGMEAFHSAFEVAENGHDPGFDWRQIDEASEFIQSHCRTWKNAG

LAASSMASESLALLWAMDTKLNTIICWNLIYILADGPTHSQVMKEIAPYSRAGRPNSGEAGFQLPEPPRLSLDADRLVDS

CPLLKATYYETLRLHSCPVTYQKITEDFKLTESAADAKIANRKQQTYEFRKGEYIAIPHALHNTDSHYFGDPEEFYPERF

LMQKD

(heme region is missing )

VDSNKPSGKGATSEKR

ESLSQPELKIPEIWTVESGGGDTVIQDGK  177525

177526  FAERVTLGFVSGLLAMWDIQPVKGMGWKIPKQSTGGLVYEPKTDIRVRIKSKV*  177687

 

>CYP658A1 Aspergillus nidulans AN8438.1 poor match 26% to 640A1 7 clan?

MPPYVIVSELLLIRLVCGLVGYVLWRLWRFTVHPLLQPNLPRELPYTIPF

IGKDLRPSQTIQSTDSLRPCNFPATEPARFNIVYHDALEVLRDRPEVINT

EHIKDLLHGLGCSKPGILEIDSHQRGNISVPDSKAALTNRPPLFKVAEGL

LSKQLLDRSLGNDLLARTLEVLETQGIPQVSLMIMSRWTNDLWKLLFKVP

EPFARDVYTARTRVQRCLQEFLRLPESEKSDQSWAIEAAVAEMRSRGMSE

ADTASYLLVVFWAINANTFKIIFWLIAHIISNPTILTAVTTEISSILSDW

PSNESLSSLSIRLERNTLLEALHNEAHRLALNTSSARTMKQDVNINGRIF

TSGTHAVIPYRYLAMRHPLLSQDWDAFQPERFLSNPGLGQSKSFLPFGGG

KHKCMGRFLSRRLVLTFTSLFLHRFKVRPLDGVPKMGFRPVSSGPASPVD

DMRLVISHKEY*

 

>CYP659A1 Aspergillus nidulans AN3349.1 poor match 25% to 640A1

lower half 7 clan, revised 7/19/07

MDILDVASSELYTLVRSLVTSPVCQGVLFSLLT

WRLWRFTISPTLQPEDPKEAPYWIPCI (2?)

NQLGNTREPFALTLAGKTMYIIADPKDAAEL

IRNSHSLSTLTTELYTRMGIPQAVIERLFTVYPDAPFNARSNARPVHATD

AMIEMYRAHLSPGAQLDEFLERDVTKRILNAFAKIPGLFNK ()

GIVGAYYGDLIFSLNPGFIAQFMVWEKVNWKLLFGLPSFLSGDMLAARKGLVDGFVAYF

ALPRAERGHENYWVKGVEDSLRWLNVSNEDIARIFMLQTWA ()

ILGNMYKMTFWLVAHILHDAALVNAITAEVRPA(0)

IEIDHHYLSEQCPKLDSLFSEVLRLTLTAPMARDVSETTTVGGRRLREGNRVL (0)

VLYRQLHLDRAT

WGPTPQTLQPDRFLIDSGLKSSIAYRPWGAGKHICPGRFLARSAVFTFVA

YLLAGFEVRLRETGTGKSTRSAFPMADMSRPSPGIANIADGEDVLISVIRRDRSS*

 

>CYP659B1 Talaromyces stipitatus ATCC 10500

ABAS01000005.1 50% TO CYP659A1

MSSSKFFSLLAPTTDDLSSFFFPSFSIAIGLLLC

WRIWRFSIRPLLHPDDPKELPYWIPCEKSSQSCNRK (0?)

272214 NGSSKEPFAITMAGKPIYLLTNPKDIAETYRHEDTLSMDMVVKDLYSRTGISMDKVNRLFIANPES  272411

272412 PHNKGLPRPLHYIDISMEYFRWELSPGESLDRFVNEAAIPAILKAFDFD (1)  272558

GVVDIYYGPALWKINPDFVHSFMMWERVNWKYIFQLPGFLSKDMLEAKRDLVDTFVTYFE

MDRTERDCGNLFVNSVEDTLREAGMGNEEIGRIFFLHSWA

ILGNMYKTAFWIIAHLVYNPSLLEAIRIEVTPAI

EVDHQYLSKDCILLDSLYADVL

RATMSSPMVRDITETTTIGGKKLRKGNRIM (0)

VSYRQLHLNTDVWGPTPELVQPDRFLRDKSLKTNNSYRPWGGGSTLCPGRFFAKKTIFTF

ISVLLVRYSAIHIKEETVGDNKGGRKPAFPRADFSKPIPGIVSPMMGDDISLILRESD*

 

>CYP660A1 Aspergillus nidulans AN4117.1 35% to 636A1 cyp7 clan

MLEPFHLLILGAITAIGYILSQRGLDPREPPEVHSKIPIIGHILGLMRHG

MGYFSRIAAENSSHPIFTINLLTNKQYIVTSPSLMQAVQRNKRTLKFAPL

VNFTAERFAGIPESGMSLLRDREAGGAGLSAGTVHAMEKTLIGPSLDRMN

EEMARMLSPLVDELVASPQTVDLYAWCTRAITAASTNASYGPKNPYKDSA

IADAFWTFETNIAPLITGVFPSIMARKAYRARETTFAAVLTYFQTKGHEQ

GSELTKTRYRVMHAGGLSDTDIARAEVSMGLGLLSNTVPAAFWVLFDLYS

RPSLLCEIRREVMKHAVRVENTQTPMGRKRIIDISALRDSCPLLVSAYQE

ILRTRSVSAVTRVVTEDTVLDNRYFLKKSGVVSIPASAIGATESVWGPES

ASEFNPRRYMRQTTPHGSTSPRRTGGFMSFGVSPTICPGRHFASSEILAL

VAMVILRIDLTPVDDGAWKAPEKNSMAIASGMCPVKGEFWVKVARRESEG

EGVEWAVEAREGSGMFNLMVG*

 

>CYP660A2 Aspergillus niger

fgenesh1_pm.C_scaffold_22000016|Aspni1

53% to CYP660A1

MLDFYTRLAVLGLLAVFGYAFTRREKPDPREPPLVRSSIPVVGHLLGFLWYGLSYFTMMTEKHAYPIFGLNMIFTKSYLV

TSPSILQSIQRNKKSLSFDPFLSMAAERMVGIRGPTLELMREKQSGGQGLKQAVIHAMQPTLIGASLDRMNERMARLLRP

LVDDLASTSTVDLYGWCRHAITVASTNAS YGPLNPYQDAKIEESFW SFESHLSPLMANFLPWLTARTAWRGREAVVAGLV

RYYEQGGHEDGSEMTQVRWKTMREAGVATEEIAREEVGMGIGLLSNTVPAAFWVLYELYSRPDLLEEIREEIRRNALRVD

ADQRHII DISAIRDNCPLVVSSFQEI LRVQSASAPTRFVMEDVMLADQYFLKAGSMINMPAATLGRMPEAWGETAREFDA

RRYMRSDEKNPRRTGGFMTFGVSPVICPGRHFASSEILGLAALLVLRYDIAPVDGQWRTPRLNSMAVASNMRPLKDEFPV

TVRPRSEYAGVQWDCTVQPGSGVFNLMVG*

 

>CYP660A3P Aspergillus terreus

51% to CYP660A2   Aspergillus niger

ATEG_00534.1

Pseudogene with defective EXXR = KTLR

Deletion near the PERF MOTIF

MPNIYALTVFGVVSALGYVLNRQQKPDLNEPPNSRQD (0)

(gap, most of N-term missing)

TFESALKPLAMKFLS*LIACK

AWQAHEKVVAALVKYYEIGGQEESSELAFVRWKTLRNGGLPIE

NIARSEVSMTVGLLSNTVPANFWVLFGLYSRPELLEEVRQEVAQKALYTSDNVRILHISTLHDDCPLLLSVFQKTLRTRT

WTSPSRFVPDDFLLTGQYSLWAGSVASMPDASVGRTP

(12 aa deletion)

RRFLTTNEDARRVCGFMMFGASPILCPGRHFASAEILAKAATT

VLLYDLTPMGGVWNVPKINTMAITSVIQPLKGGYEVTVSPRKEYEGTQWACTVREGNGMFNLMVR

 

>CYP660A4 Aspergillus terreus

66% to CYP660A2 Aspergillus niger with one frameshift

ATEG_00435.1

MLDLYRLVALGAVVALGYALSRRQHRDPREPPVVGSGIPIVGHLLGLLWYGVGYFSLMTEKYTYPIFSLDMVFGKAYLVT

SPALLQAIQRNKALTFDPFLTMSAQRIAGIRGLGLELMREKQSGGQGLNQKVVHAMHPTLTGRPLDRMNERMTQLLSPLV

DELATRKTVDLYSWCSHVITLASTEAS

YGPLNPYKSREIEDAFW (2)

AFESNLSPLTANFLPWLTARKAWKGRETAVKAVIKYYELGGHEEGSEMTYVRY

KTLREGGMSVEDTARSEVTMAIGLLSNTVPASFWVLFELYSRPELLEEVRQEVAHNALRVTADKKHVIDISA &

LRDHCPLLVSVFQEVLRTGSTTSPTRFV

LNDLVLADKYLLRSGSVISMPGASMGRNPDAWGSTAGDFEPRRFIKTDKTPRRTGGFMTFGVSPVLCPGRHFASSEILGM

AAMMVLQYDLTPVDGAWTPPPVNPMAITSITRPIKGGFDVRVSPRKEYEGVRWDCEAQEGNGMFNLMVG

 

>CYP660B1  Aspergillus fumigatus Af293

          GenEMBL XP_747896.1

          43% to 660A1

MDNLSLVVGTLILIAGSALMLRLRHDPREPPVVHNGIPFIGHMIGFVRHGIDYYALQSAKHGLEAFTMDV

LFTKIYVITTPTLVMATRRHHRAMSFEPAITGAARRVTGLKGRGFELLSEKRPGGENLHGAVVNAMRPAL

EGAGLDRMNEKVIGYLLRSVDDLPTAAPFDFHAWCRAALTLSSTDAVYGPLNPYRSKELQDAFWDFEENL

ALLLMDVVQRVTARTAWKARRRLVAAFLDYYRAGGHLDSSQLTYARWETQQKGGATLEDIARLETAAALG

TLSNTVPAMFWFMFNIFSRPELLNEVRDEVARRAVQVDAQGVHTVDLAEIKEKCPLLMSAFQETLRLRSN

TVPIRVIFEDMILDDRYLLKKGGILQMPAHAINRDRTIWGSDADDYDPRRFVKINQSDSRKKANGFLSFG

TSPHVCPGRHFATGEILALIAMLVLRYDIVPAGGVWKEPRTSKSAITSSTGPPAEEFKVLATARKEYEGV

EWAFRVTEGKGKYGLIIG

 

>CYP660B1 Neosartorya fischeri

94% to CYP660B1 Aspergillus fumigatus = ortholog

NFIA_048000

MDNLSLAVVTLILIAGSALILRIRHDPREPPVVHNGIPFIGHMIGFIRHGIDYYALQSAKHGLEAFTMDVLFTKIYVITT

PNLVIAARRHHRTMSFEPAITGAARRVTGLKGRGFELLSEKRSGGENLHGEVVNAMRPALEGAGLDGMNEKVIGYLLRSV

DDLPTAAPFDFHAWCRSALTVSSTDAVYGPLNPYRSKELQDAFWDFEENLALLLMDVVQKVTARKAWKARQRLVAAFLDY

YRAGGHLDSSQLTYARWETQQKGGATLEDIARLETAAALGTLSNTVPAMFWFMFDIFSRPELLEEVRDEVAQRAVQVDAQ

GVHTVDLAEIKEKCPLLMSAFQETLRLRSNTVPIRIIFEDTILDDRYLLKKGGILQMPAHAVNRDRKIWGSDADEYDPRR

FVKINQSDSRKKANGFLSFGTSPHVCPGRHFATGEILALIAMLVLRYDIVPLGGVWKEPRTNKSAVTSSTRPPAEEFKVI

ATARKEYEGVEWAFRVTEGKGKYGLIIG*

 

>CYP660B2 Aspergillus clavatus

69% to CYP660B1

ACLA_098770

MTLSLVVVALLILAISIVGLRHAKHDPREPPIVHNGVPVLGHVVGFIRHGINYYAQQSAKHGLDAFTMDMLFTKIYVITS

PTLVVASRRHHRTMSFEPFITGAAKRMAGLRGPGLDLLREKQFGGKGVHADVMHAMRPSLEGAALDRMNEKMIRLLVESV

DELPTVGSFDFHEWCRSAITIASTDAVYGPLNPYKAKELQDAFWDFEANLAPLLMDFAPWLTARKAWKARELIVARFLNY

YRADGHLDSSQLTYTRWEAQHRGGATVADIARLEAAAAFATLSNTVPAIFWMVFDIYSRPALLEEIRAEVTRHAVHVDAQ

GAHVVDLTDIRENCPLLVSTFQETLRLRSNTVPVRVLYEDTVLNDQYLLKKGGILQMPATTINRAPSIWGRDAAEYDAHR

FIKIHQSSDSRKTANGFLSFGTSPHMCPGRHFATGEILALTAMLVVRYDLAPKSGVWKEPKINLRAVASSTRPPATAVPM

TAMVRKEFEGVQWTFRVSEGKGRYGLIIG*

 

>CYP660C1  Aspergillus oryzae

          GenEMBL BAE63219.1

          61% TO 660C2, 40% to 660A1, 47% to 660B1

revised 3/19/2009

MSDILSIIGLTLLVAFLTSVVFFVQRRKLDPREPPLASSAIPLVGHLASFLYYGLEYFAIAS (2)

RKNRLPAFTMDMLYTKVYIIASPE

LVSAVRRSRNAMSFGPLFANVAENGGGINGRGMQLLRDKEYGGQGVGQQTADSMHPALLG

SGLDQMNGKMIAVLKTIIDELASQPDNVVDLYEWCSHAVTVASTDAVYGPLNPYRSESNRRAFWAI ESNL

SLLMMNVVPWITARKPWKGREQLTQAFIQYYQADGHLDSSQLAYTRWKVQHEAGAAIEDIARLEALTALG

ILSNTVPTCFYFLFDIFSRPDLLGKIRDEILDGAFSVDSAGVHTLDLADIRERCPIFVSTFQETLRTRSN

SGQLRVVQKDTLLDDHLLVKAGSIILMPAAVINKHPSVWGADAGTYDPERFSKIDPAQKRSKASGFMSFG

SSPHICPGRHFASGEILALVAMILVRFDVRPVRGTWVEPKGNTKAVAASLPPAVEKVEVKFSETSKFAGV

KWEFRLTPGKGTFGLITG

 

>CYP660C1  Aspergillus flavus

99% to CYP660C1  Aspergillus oryzae

AFL2G_08647

MSDIPSIIGLTLLVAFLTSVVFFVQRRKLDPREPPLVSSAIPLVGHLASFLYYGLEYFAIA SRKNRLPAFTMDMLYTKVY

IIASPE LVSAVRRSRNAMSFGPLFANVAENGGGINGRGMQLLRDKEYGGQGVGQQTADSMHPALLGSGLDQMNGKMIAVL

KTFIDELASQPDNVVDLYEWCSHAVTVASTDAVYGPLNPYRSESNRRAFWAIESNLSLLMMNVVPWITARKPWKGREQLT

QAFIQYYQADGHLDSSQLAYTRWKVQHEAGAAIEDIARLEALTALGILSNTVPTCFYFLFDIFSRPDLLGKIRDEILDGA

FSVDSAGVHTLDLADIRERCPIFVSTFQETLRTRSNSGQLRVVQKDTLLDDHLLVKAGSIILMPAAVINKHPSVWGADAG

TYDPERFSKIDPAQKRSKASGFMSFGSSPHICPGRHFASGEILALVAMILVRFDVRPVRGTWVEPKGNTKAVAASLPPAV

EKVEVKFSETSKFAGVKWEFRLTPGKGTFGLITG*

 

>CYP660C2  Aspergillus oryzae

          GenEMBL BAE56717.1, AP007154.1

          61% TO 660C1, 38% to 660A1, 56% to 660B1

          revised 3/19/2009

382760  MSGSTTIVGLSVVAILASLLFTQRAKLDPREPPLVSSTIPLVGHLISFLIYGIGYFAT  382587

382586  ES (2)

GKHSLPIFTMSILKQRVYIIASPDLLPSVRQNRSTMSF  382413

382412  NPLFTAMAQRAGGIQKPGLQLLREEELGGQGLAKKTVEVMRPALLGNKLDHLNEQMIHAL  382233

382232  KHIVHQVASSPTLPFDLYEWCSDALTVAS TDAIYGPLNPYKSEAIRKAFW (2)

DFESNLSLLLVDTLPWLTCRKAWKG REQLVQAFIQFYQADGHLSASSLAYSRWKAQQEAGASLEDIA

RLEILTGIGILSNTVPSCFWLLFDILSRPELLSAIQDEIHQNALSIDSTGTHTLDLADIRGKCPTLLSSF

QETLRTRSNSGQVRVIYQDTLLNDRWLLKAGSTLLIPAPSINKNNSTWGSDAGDFDSQRFTKIAHQTHKK

SKASGFLSFGLSPHICAGRHFATGEILALVALLLVRYDIRPIQGSWTEPKTNAKAVAASLPPAAEKFMVT

AVERPEYKGLEWRTTVTPGKGTYGLIIG*

 

>CYP660C2  Aspergillus flavus

97% to CYP660C2  Aspergillus oryzae

AFL2G_07402

MSDFTTIVGLSVVAILASLLFTQRPKLDPREPPLVSSTIPLVGHLISFLIYGIGYFATES (2)

GKHSLPIFTMSILKQRVYIIASPDLL

HSVRQNRSTMSFNPLFTAMAQRAGGIQKPGLQLLREEELGGQGLAKKTVEVMRPALLGDKLDHLNEQMIHVLKHIVDQVA

SSPTLSFDLYEWCSDTLTVASTDAIYGPLNPYKSEAIRKAFW

DFESNLSLLLVDTLPWLTCRKAWKG

RERLVQAFIQFYQADGHISASSLAYSRWKAQQEAGASLEDIARLEILTGIGILSNTVPS

CFWLLFDILSRPELLSAIQDEIHQNALSIDSTGTHTLDLADIRGKCPTLLSSFQETLRTRSNSGQVRVIYQDTLLNDRWL

LKAGSTLLIPAPSINKNNSTWGSDAGDFDSQRFTKIAHQTHKKSKASGFLSFGLSPHICAGRHFATGEILALVALLLVRY

DIRPIQGSWTEPKTNAKAVAASLPPAAEKFMVTAVERPEYKGLEWRTTVTPGKGTYGLIIG*

 

>CYP660D1 estExt_fgenesh1_pm.C_chr_10897|Mycgr3 Mycosphaerella graminicola

42% to 660A1 A. nidulans

44% to CYP660A2

MIASAISPTAAAVVALVIFFIVLVARGQPDRREPPHLTPRVPLVGHALGILCYGVPYYAKIAHQTSAPIFTLDLLVNRLY

VVNSARMVAAVQKSHKIIAFDPFLTAAANRMAGINGPGLRLLQETQAGGKGVNQEVLHAMIPTMLGSGLDQMNRTMLRKL

GPLLDDMIKDAPKTLDLHQWCQRTITTASSEAIWGSKNPFRSEKVREDFWYFESHLSILLAKIFPSVLARKAYLARETVV

KAMLEFAKSGGYDDEACSELAIARRNTQVKAGASDHDIARLETALNIGVLSNTVPSTFWTLFDIYSRPQLLQQVRSEIEE

NAVVVDPMTNVHSIDLGAIRGSCPIFVSAFQESLRVHSNGAPTRMVYEDVVLDGSYLLKAGSVLQMSAPSINSEQRHWGQ

QAADYNPLHFQKDAADVSANKPRASSFMSFGASPNICPGRHFAAAEILSVAAMLLMRVDMIPVKGYWWTPRLNAWAIAAS

MTPPIEEYPVKIVPRKEVQGIEWDFVVSGKKDRFELITG*

 

>CYP660E1 Coccidioides immitis

41% to CYP660B1, 38% to CYP660C1, 38% to CYP660A2, 36% to 660D1

CIMG_02364.2

MEQFYLHLLVLLVLSILLRFLIAPGHAPEEPPLVSSRIPFIGHLIGLARYGNRYYVRLR (2)

AQSQLPAITINIFRQKIYLIYSPGLLAAAQRHAKYISFEPFAALAIRRAAGIKGNGPIVSSSSEKGRSSFDKQMK

DAVHSVLLGEAMVGITRASVAAIKKSVDDLRSTEVDLLTWCRHVLTMATTDSIYGPMNPYRNREAEAAIWEFDSGLGKLT

LNFAQRFIAREAYQAREKLVKRLLEYFEKCGYINASSFVQRRLEVERKAGATLEDIARLETLMVLPLLSNSAPSTFWVLF

DIFSRLDLLEEIRKEIVQNALQFKPCDDSMGKPKQMCTLNMNNIRDKCPLLVATWQETLRLRANTASVRFVSKDTKLNDT

YLLKANSIVQTPANVLNVDPNIWGPDAHLYNPRRFLDTRASPGAGGDRKRDRKWNGFMTFGASPWTCPGRHFATAQILAL

VSLLVMKFDISPVAGDWVSPRINHRSMALAISIPAEGLMVNVVTRKEVKGIDWEFQMMEGKA

 

>CYP661A1 Aspergillus nidulans AN2607.1 30% to CYP56A1 547 clan = 56 clan?

MHPTLLYTCTFFITVTALLWRYFRVPSTIPSNIPRIPIWVNFYAWYHDLS

VIELYDAFYRKIMEEHGAVVVWFTGAWCILVTKPEYLVEIFRNEDTYPKV

GVNVRGKGSLMGIFAGENIINSSKPTWATLTKVMKPGFLKSFDAQAIHAK

AKKVPERLLQAQSEVGKGKGVPATQWMEKYAQDVMGLCLFNFDLQALDEP

RVPYAPLLGQIIPTIFSRWAFYFPTLDIPGRYFLSRKATLDNIAKFDSLL

NEIVESTLNADAEKQPKVVSHMLKRALDNGQLTPELYRYNLRMSFMFGHD

TTAIFMGLTMYVLGNNTVLQDRLRTEVLQSTGKTIHQLPYLTSLLYEVLR

LYPPVTEVLNHTVSRPTALGGKVTIHPGTWLGWNAYGVHTNPAIWGPDAL

EVRPERWGDTVKDIQASFRLQSTKGNYIPFSLHARKCLGQSLVLTEVKLL

MFEMVRQLKWVVDPTYKLNLGGVTFTMLLGLRLIVEELELDSRDTVADEK

SQMHT*

 

>CYP661B1 Aspergillus fumigatus

XM_742987

38% to CYP661A1

shortened at N-term

MILYYLASIPLAIICYLAWYLHVPWDLPSLPRIPFYVSILGLWSSMGQDEIYERWLRKP

LEAHGAVLIWFAGRWSILVTRPDLLTDMFRNEDLYAKAGSQKKIPWSVIATLVGDNII

NSHGDTWKLYTGIMKPGLQKKNFDTAPLLLKSRRFVDEILAEQNSAGRGTGILVNTFV

QQWAVDVMGMSFLDLDLQSLEKPHGTVRLEAIQSVIKLMLFRPLFFNFPDLDQFAWLI

KSRQRAYEIMHEFGDTLMATVLGRIDSDREKGIKPAEEMVVHMLVDAYRDGRLTEKQF

KDNLKIVFLTAHENAQQLVNSMFWEIGKNNEVQTRLRAEILSTNTTTPTSEVVNALPY

LTAVVYELLRLYPPVSQLINRVTVRPAMLGNEIPIPAGTFVGWNAYGVHVNPAIWGPD

ANEFKPERWGRTVGEMHARFRRETVRGTYIPFNAHSRKCLGQGFVLLQMKILLFEVLR

RIEWTVDPGYRLKMTPVSYFLESGRKSTDA

 

>CYP661B1 Neosartorya fischeri

98% to CYP661B1 Aspergillus fumigatus = ortholog

NFIA_039460

MILYYLASIPLTIILYLAWYLHVPWDLPSLPRIPFYVSILGLWSSMGQDEIYERWLRKPLEAHGAVLIWFAGRWSILVTR

PDLLTDMFRNEDLYAKAGSQKKIPWSVIATLVGDNIINSHGDTWKLYTGIMKPGLQKKNFDTAPLLLKSRRFVDEILAEQ

DSAGRGTGILVNTYVQQWAVDVMGTSFLDLDLQSLEKPHGAVRLEAIQSVIKLMLFRPLFFNFPDLDQFAWLIKSRQRAY

EIMHEFGDTLMATVLGRIDSDREKGIKPAEEMVVHMLVDAYRDGRLTEKQFKDNLKIVFLTAHENAQQLVNSVFWEIGKN

TEVQTRLRAEILSTNTTTPTSEVVNALPYLTAVVYELLRLYPPVSQLINRVTVRPAMLGNEIPIPAGTFVGWNAYGVHVN

PAIWGPDANEFKPERWGRTVGEMHARFRRETVRGTYIPFNAHSRKCLGQGFVLLQMKILLFEVLRRIEWTVDPGYQLKMT

PGGILAPLGCRVILSEVKEADKAEQRG*

 

>CYP661B2 Aspergillus clavatus NRRL 1

AAKD02000007.1 ctg02_1099423829543, whole genome

ACLA_002540

gc boundary After GKNT?

82% to CYP661B1

575568  MSLFYIFAVPLSLIILYLAWYLHVPWNLPRLPRIPFYVSILGLWSDMGQDEIYER  575732

575733  WLRAPLEKHGAVLIWFAGRWSILVTRPDLLTDMFRNEDLYAKAGSQKKIPWSVIATLVGD  575912

575913  NIINSHGDTWKLYTSIMKPGLQKKTFDSRPLLVKSRRFVDKILEDQATAGK-QGILVNAY  576089

576090  VQKWAVDVMGVSFLDMDLQ  (0) 576146

576245  SLEKPHGAVRLESIQSVIKMTLFRPLFFNFPDLDQFAWLLSSRKRAYEIMHEFGDTLAD  576421

576422  KVLAQMDDATAKGQKPEEEQVVHMLVAAYRDGRLTQKQFKDNLKIVFLTAHENAQQLVNS  576601

576602  MFWEIGKNT (0)  576628

576739  EVQTRLRAEILATGTSNPTADIVNPLPYLTSVIYELMRLYPPVSQLINRVTTAPAMLGGQ  576918

576919  IPIPARTFVGWNSYGVHVNPQVWGADAQEFVPERWGSTVSEMHARFRRETVRGTYIPFNA  577098

577099  HSRKCLGQAFVLLQMKILMFELLRRVEWRVDPGYKLKMTPVS  577224

        SHSSFCFVFFYYVACEV* 577278

 

>CYP661C1 Histoplasma capsulatum G217B

47% to CYP661B1

HCB03363.1

MALLACVGAAAVLFAITAWWYLRVPSHMPKNIPTVPFYVSSIAHFVDLGQDKIYDLWLREPLEKYGAVKFWVSSQWTVLL

GKPEYINDLLRNEQVFPKAGNHKRIPFSVIGTFLGNNIISAEGETWKLYTSIMKAGIQRRITDTSKLLGRSRQLVRGILQ

SQQAATANFGIDLESLVQMYAIYVVGEHFFQTDFQRLECNEATRLAKYQTAIRGNVFSPLYFSFPILDKYPYIFQSRKRA

FRIVKEYEDLLYEIVRVPRPRETPEDPMNQYERQVIDDLRDALDCRKITEVQFRANVKIIFVAGHEDVQHLLNSAFYEMG

AKTDIQQKLRLEVLNTGSIDPTPEIVDSLPYLAAIVFELLRLYPPLQQIINRKASETAVLGDEIVISPNTLIGWTAIGAH

TNKSVWGGDAREFDPERWGTTVDTIRAKFRRENARGAYIPFNAHARKCLGQGFALLQIKIALFELVRNVEWVLDPGSKMK

LARVSNYKISKAASPHRITINQSCVTGFIEAYRL

 

>CYP662A1 Aspergillus nidulans AN9210.1 33% to 59B1 59 clan

revised 7/19/07

MLALLLVLVALTSYFLVRMRQQRGMFRNLPGPPHHAIWGHFLIMRDIASS

LPPDATPQLFAHLMRQRYGLGDFFYLDLWPLAPPQLVIAHPELATQIVHK

MNLPKESAVMQKWTGPILGEKSMVSANGHDWFIARKSFTPGFQPRKLQQH

IPHIVDETQAFADILREHFHMNDIFKMEDLVARMIFNISAGVILGIKCNA

QRDDDEFLELFRKQAALAPQDFWSRYLYDVSPRRYYRKWSNGRALDRYVG

RLVDQRVVSGPAANPDQKTKYYAIDDAIATSRTLNKNIPHTSSLDKYTRD

MLITSVKTLIFAGHDTSASTLCYTFAAISKHPQVLNTLRKEHNALFGTDP

SAAADLLRDDPNLVNNLPYTLAVIKEALRLWPPTGISLRRGQPDQTLFAE

GKEWPTYPFAVLVNNCATMRREDLFKDAERFYPERHLVNDPTDPYFVPRD

AWRPFEKGPRMCLGQTLALMQLKIALVMTVRTFDFEIVYEEGTFMYQVLD

VTAKPSLGLPTRVKLVE*

 

>CYP662A2 Neosartorya fischeri NRRL 181

89% to CYP662A1 Aspergillus nidulans

NZ_AAKE03000038.1, NFIA_044230

Note: this seq does not have an ortholog in A. fumigatus

MLALLLVLVALASYFLVRLRQQRSMFK

NLPGPPHHAIWGHFLVMREIASTLPPDATPQLFAHLMRQRYGLGDFFYLDLWPLAPPQLV

IAHPELATQIVHKMNLPKESAVMQKWTGPILGEKSMVSANGHDWFIARKSFTPGFQPRKL

LQHVPNIVDEALAFADVLREHALKDDIFKMEDLGARMIFNISAWVIL (2)

GIKCNAQRGDDEFLELFRKQAALAPQDFWSRYLYDVSPRRHYQKWSNGR

ALDRYVGRLVDKRVVSGPTAVPSEKARYYAIDDAIATSRTLNKSIPPTTALDKYTRD

116369  MLIASVKTLIFAGHDTSASTLC (0)

116479  YTYAALSKHPQVLHTLREEHTTLFGTDPSAAANLLRNDPSLVNNLPYTLAVIKEALRLWP  116658

116659  PTGVSLRRGQPDQTLLADGKEWPTYPFAVLVNNCATMRREDLFKDAERFYPERHLVTDPS  116838

116839  DPYFVPAKAWRPFEKGPRMCLGQTLALMQLKIALVMTVRTFDFEIVYKDGTFMYQVLDVT  117018

117019  AKPSLGLPTRVRLTE*  117066

 

>CYP662A2 Aspergillus terreus NIH2624

AAJN01000191.1

90% to CYP662A2 Neosartorya fischeri = ortholog

ATEG_07945.1

        MLALFLIPVALV

267630  SYFLVRLRQQRAMFKNLPGPPHHAIWGHFLIMRDIASTLPPDATPQLFAHLMRQRYGLGD  267451

267450  FFYLDLWPLAPPQLVIAHPELATQIVHRMNLPKESAVMQKWTGPILGEKSMVSANGHDWF  267271

267270  IARKSFTPGFQPRKLLQHVPNIVDEAVAFAGVLREHAQRNDIFRMEDLGARMIFNISARVIL () 267085

267032  GIECNAQRDDDEFLDLFRRQAALAPQDFWSRYLYDVNPRRYYQKWNNGRALDRYVGQLVD  266853

266852  QRVVSGPTSIPSDKAKYYAIDDAIATSRILNKTIPPSTSMDKDTRDMLIASVKTLIFAGH  266673

266672  DTSASTLC ()

266602  YTYAALSKHPQVLHTLREEHKALFGAEPSAAANTLRSDPSLVNNLPYTLAVIKEALRLWP  266423

266422  PTGVSLRRGQPDQSILADGKEWPTYPFAVLVNNCATMRREDLFKDAERFYPERHLVTDPA  266243

266242  DPYFVPRDAWRPFEKGPRMCLGQTLALIQLKIALVMTVRTFDFEIVYEEGTFMYQVLDVT  266063

266062  AKPSQGLPTRVRWIE*  266015

 

>CYP662A3 Aspergillus terreus

52% to CYP666A2

ATEG_09350.1 this model is a fusion protein, revised

MFQSFGIALWGFLALYPIHVTLEFFKNRRLAQQIGLPYMVFPFSEHSVFYLGFVETRWYRYLVDHCLPAWLADLVHNSSF

KYRWRVKDRITKLYGPVYTIVTPWSVTCHVSEANVVTQVCMARRSFPKPVKNYEGFAIYGPSVLTSEGNQWARYRRYTSA

TFNEKNNALVWQESIRQGIQMTNYWEEKHASDSPHTLFSLPDVRDDIVKFTLNIICSAGFGVTLPFKPAYKATTGDAEGL

FQDAVMPPQGYSFTFPSVMEYISTSMSTVFFANGIWPKWIPRRMAPFFKTDFMAYDDLGKYLRALLDNAEVRENPSSHNL

LEGLVRARRENSELGSTHRVDGLSDEEIFGNLYIFMIAGHETTATTLRFALVLLAMHQDVQDHLYDEIQEVTMGEPANPT

GWDYERTFPKLVAPLCIMLETLRLYPPVSSLPKWTDTSSASISYRDQAYQIPPATYLCLDANALHYDEMYWGPNASEFDP

SRWDKRNIDSFLAKNDGLDGLSGPGLEHDTIHRPVRGAFLPFSDGLRACVGKKFAQVEFVAALVIIFRNYRVTLQKHADE

SESEMRCRVQQALEQSSNAQTLTIGENVPLVFSRREA*

 

>CYP663A1 Aspergillus nidulans AN7399.1 35% to 546B1 64 clan

MTADIKTLLLAALGAVAVAWLAQSFGKRSSLPLPPGPKPALLIGNIHQLP

KSLQWLHLYHLSKEYGPIMHFSMAGQPLIILSTHQVAHDLLNRRSGRYSD

RPRMVMAGELVTKNMHMLLRPYDERYKLHQRMEAPLLVLRSASNYRPLQD

LESQQLLFDVLGEWDKFGEKGVDFHHHFERAMASTIYCLNYGYRLQTGYE

KELMDGKKVQAEFARTGQVGAYLVDSFPSLNYLPKFLAPWKKEGEGLYEL

ERQLHVGNLKKGLSNRGWNFTKYMKDSPEAVTMPEEELAFDLGILADAGL

DTSTVALDWFIVAWITSGSTWVKKAQQLLDEVVGKDRMPTFEDRPKLAYI

DAIASETLRWRPVVVSGVPHFTKVQDEYMGYHIPANSTVLPNAFAISHDE

SIFGEDVDSFIPERWLAEDPPSEPSIDACGFNTSALKDLPHIGFGWGRRI

CTGRFIARNQLFIQMARMLWAFDVDAGVVDEKTGRRHNVDDMDCTEGFVT

LPKPFRAVMRPRGEWVRQRILERGTTHGLDHAKILQNAKLGRS*

 

>CYP663A2 Nectria haematococca

e_gw1.12.597.1

Necha1/scaffold_12:54695-56385

80% to 663A1 AN7399.1

MLLQDRRSLGLAVVGAFVAWLLKRYLSGRGKGPLPPGPKPAPVIGNIHQLPKSLQWLHLYRWSKEYGPIM

HLSMGGQPLIILSTHQAAHDLLNRRSAQYSHRPRMVMAGDLVTKNMHMLLRPYDERYKLHQRMEAPLLTL

RSASTYRPLQDIESRQLLFDVLGESNQAGDKGVDFHHHHERAMASFIYCMNYGYRLKTGYEQELMDGKKV

QAEFARTGQVGAYIVDSFPSLNYLPKFLAPWKKEAEELYELERQLHVGNLKKGLNNRGWNFSKYMEASPE

ATDMPTEELAFDLGILADAGLDTSTVALDWFIVAWITCGERWVGKAQKVLDEVVGRDRMPTFEDRPKLAYIDAI (1)

ASETLRWRPVVVGGVPHFTKKEDTYMGYYIPANSIVLGNAYAITRDESVFGEDVDAFLPERWLADD

ANTKEPNIDACGFNTTALKDLPHTGFGFGRRVCTGRIIARNQLFIQMARMLWAFNVEAGINEATGEKHKV

DDMDCTEGFVTLPKPFKTVYHPRGQWVRDTIAESGTTHGLDHAAILDGAKKDRAKA*

 

>CYP663B1 Aspergillus niger

e_gw1.10.193.1|Aspni1

43% to CYP663A1

MPTILLTLLALLTLRLLYEYKRDRNLPPGPRRLPLIGNLHQAPQTLPWRVFDQWSKTYGPIMSAQFGRQTLVLITSPTIA

RDLLDKRGSIYADRPDLVMANNITKGLHMLIRQYDDWLRLHQRLDAPLLSPRASNTYHPIQDLESKQLMFDLLRSNDFDA

HFERYSGSLMFALAYGFRLLSPKGQELRDMRTIQGNFTYAARVGTWIVDAIPVLNYLPAVVATWKRLAEKLFKLEASVHT

RHLEKGLNSEPWNWSKEFAASKHAEGMPRLDLAYNLGILVDAGFETTWAVMKIFVLAIRSDSRFVAVARKELDEVVGEDR

MPTFEDQEKLVYIQAVVDETLRWRSMAPGGIPHATRKEDTYMGYRIPKGATVIPLFWSMSLTDEPWDDPLEFRPERWFEA

TEKEEGRFRNFFGYGRRICTGRHIARNSLFLLMARILWAFDIQAPLGDDGKPVPVDDMAFDSAFVSTPEPFEALFVPRSE

KTKEIVEREWNEMEKDMAVLMGQVRESQR

 

>CYP663B2 Aspergillus clavatus

60% to 663B1

ACLA_078190

MISIYILVGLVLLRLLYEYHRDRNLPPGPRRLPFIGNIHQVPQSHPWRVFDAWSKRY GPLMRAQFGRQTLILIADTRIAR

ALLDKRGAVYSDRPRMVMAGENVTRGMHMLLRPYDARYRLHQRMEAPVLSPRASATYTPLQELESTQLLTDLLRSNNFTI

HIQRYTASLVYGLAYGFRLPTGAEPELLTLRRILHNFGHVARVGTWLVDAIPLLNYLPAWLAPWKRSANALFELEADLHR

RNMDTALHNKPWNWTKELARSPHATPMSPLEFAYDLGNLTTAGYDTTASAMRVFVLAAVTAPAFIAAAQRELDTIVGPDR

LPSFADKENLP YIAAVVEETLRWRSIAPGGPPHATSAEDEYMGYRIPKGATVMPLHWSMSMDAGDFPDPLAFQPERWL

AAPQQQQQQGGDGKGNEHE

RFTSFFGWGRRMCAGRHIARNSLYILIARLLWAFEIRAAEGPDGRPKHVDDMAFDDGLVSSPE

PFEAVFVPRSEAVRRVVEGEWAGLEKDMEVVMGGIRERQRAIGLEVRV*

 

>CYP663B3P Aspergillus oryzae

Supercontig 6: 3781646-3782486 (+) strand MAYDNLLLHWWKRNQSINESPPLPLSCIAGCVSLAHVLTQKKLIQDTKM

EFGILNSFLALLILRVIYEYYRDRRLPPGPRR

LPLIGNIHQVPQVLPWRTFHQWSKKY

(large deletion of about 274 aa)

IRVVVEESLRWRSIVPGGVPHAARKDDTYMSYHI

PKGATIVPLHWSMSLDEQHFDNPLEFRPERWLAEPDDDRFTNFFGHGRRICPGRHIARNS

LFILVARILWGFEVRPPTGPDYQPKIVEDMDFGSAFVSVPAPFEAIFQPRSENARRVIES

EWESTEKDIIALMDSIKEK

 

>CYP663B3P Aspergillus flavus

95% to CYP663B3P Aspergillus oryzae

AFL2G_01674

MAYHYLLLHWWKRNQSINEPPPLPLSRIAGCVSLAHVLTQKKLIQDTKMEFGILNSFFALLILRVIYEYYRDRRLPPGPR

RLPLIGNIHQVPQVLPWRTFHQWSKKY

(large deletion of about 274 aa)

IRAVVEEGLRWRSIVPGGVPHAARKDDTYMSYHIPKGATIVPLHWSMSLDEQH

FDNPLEFRPERWLAEPDDDQFTNFFGYGRRICPGRHIARNSLFILVARILWGFEVRPPTGPDYQPKTVEDMDFGSAFVSV

PAPFEAIFQPRSENARRVIESEWESTEKDINTLMDSIKEK

 

>CYP663B4 Aspergillus terreus

64% to CYP663B2

ATEG_07324.1

MAITILTSILGIFLLRLIYEYQRDRKLPPGPRRLPFIGNIHQAPQILPWRTFHEWSKKYGPIMSVQFGRQTIILIADATI

ARELLDKRGSIYSDRPRMVMANENLTKGMHLLLRRYDERYRLHQRMEAPVLSPRASSTYYPIQDLESKQLLYDFLSSNNF

FKHFERYSASLVYTLTYGFRLETGEEQEMKSAREVMHNFGLAARPGTWIVDAIPALNTLPMCISPWKQTAEKFFQIEANQ

HMRNMTVAKENKVWNWTKEFVVSKQAEDMSPLELAYDMGILADAGLETTSGVMQTFTLALLSYPGFIKTAQEELDRVVGP

DRLPTMSDRENLPYMTAVVEETLRWRSMAPGGVPHATLKEDTFMGYRIPKGTMVMALHWSMSFDERHFENPLEFHPERWI

GRNPDDGCFTNFFGYGRRVCTGRHIARNSLFLLMSRILWAYNVQPGTGPDGKPKKVDDMAYGSGFVSIPLPIRRDL*

 

>CYP663C1 Mycosphaerella fijiensis

50% to CYP663B4 Aspergillus terreus

gw1.8.660.1 revised N-term

MIANIIYSITLLILVRILYEYTIRDRK

LPPGPRRLPLIGNLHQVPAAGQLPWLVYHQWAKEYGPIYSLQLGGTTFIVISSGEIARDLLDRRGSRYSDRLESVMAGEI

LTKGNHILLRPYDGTYRLHQKMEAPVLNSKTSPLYAPVHELESLQLLKDLLSTNDSHSFAEHLERYSISIIYSLAYSLRV

PDFTHPDVATIHETQAHMLDCLVPGKWLVEVIPAMKYLPRCLAPWRVIAENYHLIESNLHLKHMDYGLSTQSWNWTKEFT

SSPQGTDMERLEMAYDLGILADAGMDTTAAILQTFVFAAVTQPSFLVSAQKELDSVIGQHRIPMLTDKAKLPYIMSIVNE

TFRWHTVVPVLTPHATKVEDESHGYRIPAGSCVLALQWSMNMDETSFPDPHTFSPDRWLDGLDAKKKKFHAFGYGRRICT

GRHIAENSAFLVIARLLWAFEIFPG*

 

>CYP664A1 Aspergillus nidulans AN9222.1 37% to 530A1 66 clan

revised 7/18/07

MTMAALDVFNVPYSVPLLGSTVVILIGFIAIKALRVGSRPKGLPPG

PPTEFIWGNTKQIDLFYPQYQYRKWAQQYGPVYTVMLGDTAHVVVSGLRD

VRDIFIKQGASSQNRPPSRFQLLMRDGFFPGLNNGEKWRQSRRMWQAVLN

NSAAKQYLPYQELETRQLLFDLLRAPTEWRDHIERYSNSVAMTMVNGRRI

IDAADPRVKETIQDLYDLAETGVRGAFLDSWPFLWKLPEWMFPVCRQARK

IAAKHREYIWRNYSDVAKRTSQGEVLPSVNHAIQEKLKQGWPGVSEIEGA

EIGHHLLTGTTDTTASTLINWVAAMCLHPEAQKKAQEEIDRVVGPNRLPT

DADAANLPYVQQVIQEAQRWITAVPLSLPRAANAPVHWGKYTIPEETGLI

MNSHALHNDPDIFPEPDKFKPERWEGKPNASSNGDAQLLFTFGAGRRVCP

GQHLAERSLFLVISHWLWGFDTLQATDDDKNKIPIDKDDLRPGFIVCLNP

FPAKITPRTAQHRELIERIWKEELEVSLDESQQWKATPEGIARLLERVGK*

 

>CYP664A2 Uncinocarpus reesii

58% to CYP664A1   Aspergillus nidulans

UREG_03024.1

MATIRRALPFSTATLGCVVLIAALSYLLYRLLGIGRRPAVLPPGPPTQAIWGNLRQCDSFFPHHQYTEWASKYGPVYTMM

QGGQPQVIVSGLAEAREIFVKQGARTQSRPPSRFKLLMRGGYFPSVMNGLKWQHARRMWHAVLNSSASSKYLPYQELESE

QLLLDMLNKPHCWYDHLERYTNSVGMTMVNGYRITSSDDPIVKETIEDLYELSRVGVRGNVLDLWPVLWKLPIYLLPICR

EARELAEKHKRFIWRNYTAVKESVKQGTTLPSFNRTIQERLQSGWRGVSEIEGAEIGQHLLSGATDTTVSVLTTFVAAIC

LFPGVQRKAQEGSERLPQYEDTASLPYIQQLILEAQRWITSVPLCLPRVANGTVRWGNYEISEGTGLIINAHAIHSDPGM

YPEPHIFQPERWEGKLDATRPDNQLLFTFGAGRRHCPGQHLAERSLFMVVSRWLWALDTSQAKDKDGNDIPINTKDLRPG

VINRLKPFQADIKPRSPERATLIRQVWRENCEYLLDQKHQWKLNPDINYKRVK

 

>CYP664A3 Coccidioides immitis

66% to CYP664A2

CIMG_02812.2

MALTHRVLPFSAGTLASVAVSAALGYLIYRLLRLGRRPAYLPPGPQTQAIWGNLKQ (0)

CDPSFPQHQYADWALTHGPVYTVMQGDTPQIIVNRAAEARDVFVKQGAYTQNRPPFRSIMLLRDGY

FPSLMNGSKWQNARKMWQVVLSSSAIKNYIPYQELESEQLLLDTLNEPLLWYDHLERFANSVGMVMVNGYRITSSSDPIV

KETVEDLYDLSRVGFSGNMLDFWPFLERVVWTLPIYKVGRRMAKKHKDYIWKNYFGVKQRAEQGTRLPSFNRTIQERLQ

EGWTEVSELEGAEIGQHLLSGSTDTVVSTLTTCIAALCLFPDVQRKAQE (1)

VVGPDRLPRGEDEINLPYIQQLLLE (2)

AQRWCPAMPLCVARVTSGPVSWGKYNFPENTGLTINTYAIHNDPELYPEPH

IFMPERWEGRIKAAHGENQLLFTFGAGRRFCPGKSLAEKSLFIAIARWLWAFNTSHATDEEGNKIPIDPKDLRPGNIVRI

KRFSADVQPRNPQRAGVIRRVWREKCDTLLDENHQWKTNPERIAESMKMECDI

 

>CYP665A1 Aspergillus nidulans AN2727.1 35% to 530A2 66 clan

MGLSLGNDLWILLVSLLVAIVTSLVVALAPYTWASLRPKNFPPGPKPLPL

IGNLNLIPPSKAFLLFHQWTKKYGPIIGLKFGPTNVVVLNNWKDVQELLE

KRGHIYSSRPDNYIANELICKNHTHILFAPYGDGWKALRKAAQGLFTPRE

LAGVLPIQEAEATQTAFDLIRSPDRYYEHIQRYTTAVILASVFGQRGADF

NSPNVQALYDVQNRFTALLEPGAAPPVDGITFLRHIPEFLAPWKRRAKEI

RRDQRALYFRLYNATKERMAKGIRTGCFMEKLIDDQVKNGLDDEHTTYLG

GILMEAGSDTTSSTLLSFLLAVLENPGALKRVQEDVDRVVGTERSPTMND

LENLPYIEACMHEILRWRPVAAGGIPHMLTQTDTYKDYIFPAGTIFFANT

WAIHHDETEYSNPAIFNPDRWLDGNKYGTKDNNSTLDSTAQRKTSYGWGA

GRRICSGQKMAEASLKINIAKLVWAFDFERVKGEENVDISVDTGYEGGFL

VCPKKFPIKITPRSEGRAAVIEKEFEGLKGFYEKLAA*

 

>CYP666A1 Aspergillus nidulans AN1748.1 poor match 534clan

MLVFTIPLATALIYITHSAFSFLSNLYLVCKTCPSLPRVLFPVSEVNLLY

LALFESRWFNHIRRYWLPTSIADYIADAAFRGRWDVKDRLARKYGGFYLF

VAPGLISCHVGDAEVVSQVVKERKGFVKPVKHLEAFEMYGRNVLTSEGSE

WAYHHRYSASAFSDKNNGLVWQESAIQAQEMLAYWEKKFNASNGSDRFTV

PDVREDILKLSLNIICSAGFGVRLPYRPATSATAERDKDSAEDLFRDAAT

PASGYHFTFRGVMEYMNRSMMSVFIANGILPKWIPRMLVPFFKTDFAAHE

DLKKYLHALIEVTENSEHERHNLLGRLVAARREEQSVRCSGSGPGLSNAE

ILGNTYIFSLAGHETTATTMRFALPLLAIHQDVQDRLYKELEEALRDQPA

NPAEWEYSTVFPRLVTPLC (0?)

LETLRLYPPVVSIPKMTTTHMAEITYKGERHCLPPNVRVNLNANVLHY

SEAYWGPDADSFDPRRWDKRNAGSFLARNASARGLSGPGLESPNVHKPVR

GAFIPFSDGLRACMGRKFAQVEFIATLAVIFHRYRVTLVRVGQETEDDAR

KRVEKALRQSSTLITLALGGVVPLAFVRRTGVAAEDDA*

 

>CYP666A2 Aspergillus oryzae RIB40

AP007151.1 genomic DNA, SC005

50% to CYP666A1 Aspergillus nidulans

join(3144893..3145283,3145344..3145394,3145416..3146254,

3146318..3146493,3146532..3146865)

model wrong, frameshift at 3146521

3144893 MLGIILFSLFISYTAYLGLCFLRHWSLARKIGLPYVSFPISSHN

        ILFLSLFETRFVPYVINTWLSPKLADFIYGSAFKTRWAARDRLHRRYGGVYMLITPSV

        STCMVCDASVASQICMSRHGFPKPIKQY (1) 3145283    

        GALEMYGPNIVT (0)

3145445 SEGSQWAHLRRHTATPFNERNSALVWEETIRQTKEMVQYWEDEYSRSSSASEFILTDTRED

        ILKFTLNIICSVGYGVKLPFRPGLENSTESAEGLFKDAITPLPGYHFTFRSAMEYLNK

        HITSMFIANGLLPKGIPRSVLPFFKKDFDAFDDIGRYLRALVSTAETKETLSQNLIDG

        LVRSKQKIDKDQGLDPELTEDEILGNLFVFTIAGHETTAVSLRFALVLLALNQDAQEY

        LYEGIREATYDEPRNPVEWDYRRVYPKLVSPLCVM (0)

        LETLRMYPPVSGIPRWTGDSAVN

        ITYHNQPYLLPPHVYVNVNASGLHYSEEYWGPDAAV FDPKRWDKR  3146521

3146521 NTRSFLAKNKEGGLSGPGLEYDTIHK

        PVRGSYIPFSDGFRSCIGKKFAQVEFVVAMAIIFREYRVMLAKSNERETEDDLRRRAE

        KALGESTAFITLSMRDEVPLLFQKRCVSKA* 3146865

 

>CYP666A2 Aspergillus flavus

99% to CYP666A2 Aspergillus oryzae

51% to CYP666A1 Aspergillus nidulans

AFL2G_01122

MLGIILFSLFISYTAYLGLCFLRHWSLARKIGLPYVSFPISSHNILFLSLFETRFVPYVINTWLSPKLADFIYGSAFKTR

WAARDRLHRRYGGVYMLITPSVSTCMVCDASVASQICMSRHGFPKPIKQY

GALEMYGPNIVTSEGSQWAHLRRHTATPFNERNSALVWEETIRQTKEMVQYWEDEYSRSSSASEFIL

TDTREDILKFTLNIICSVGYGVKLPFRPGLENSTESAEGLFKDAITPLPGYHFTFRSAMEYLNKHITSMFIANGLLPK

GIPRSVLPFFKKDFDAFDDIGRYLRALVSTAETKETLSQNLIDGLVRSKQKI

DKDQGLDPELTEDEILGNLFVFTIAGHETTAVSLRFALVLLALNQDAQEYLYEGIREATYDEPRNPVEWDYRRVYPKL

VSPLCVM (0)

LETLRMYPPVSGIPRWTGDSAVNITYHNQPYLLPPHVYVNVNASGLHYSEEYWGPDAAVFDPKRWDKQNTRSF

LAKNKEGGLSGPGLEYDTIHKPVRGSYIPFSDGFRSCIGKKFAQVEFVVAMAIIFREYRVMLAKSNERETEDDLRRRAEK

VLGESTAFITLSMRDEVPLLFQKRCVSKA*

 

>CYP666B1 Aspergillus fumigatus Af293

XM_741694.1 cytochrome P450 monooxygenase (Afu7g01450)

44% to 666A1, 48% to 666A2

gene model wrong, correct with genomic DNA from AAHF01000015.1

revised at VM boundary 3/11/2009

      MIWSSVLLGLAGLYAFDYFRRLFNNIR

82    LAQRTGLPYTVLPFAGNSLQVKILLSIRWLPYIINHWLPGWLADIINDETYDYRWTVKDR  261

262   RAKKLGKMYMVVTAENIVCHIADASLVTQICNARQSFPKPIWQY (1)

      ELLNLYGPNLLT (0)

      CEDQAWAHHRRHTAPTFNEKNSALVWEESIRQMTEMLHHWQVAGTSEKLHGFVVASTRGDLLK  543

544   FSLNVLCGAGFGVKLPFKQLPQESTNDPNDVFKDTEKPPEGFSFTFRSAVAYMNLRIMTV  723

724   VLATMVIPKWIPRALMPWLKSDFEAHRDLEAYLRKLISMGKAEKTADKPDNSQNLVQGLL  903

904   MSRNKEAASGSSKGIGLTDLEIIGNMHIFTIAGHETTATSLRFTLLLLALHQDVQDWL  1077

1078  YEGVLEAAHGEPKDVAQWDYHRMFPKLITPLCVM (0)

      LEVLRLYPPVVTVPKSTSETPSPLTY  1257

1258  QGKQYVLPPRVNINLNTNCLHYSEQYWGPDVAIFYPQRWDARNQNSFLAKNASTQGLAGP  1437

1438  GLEFPTVHKPVRGAFIPFSDGFRACLGKKFAQVEFIAALSALSRNYKVELADDSPEGRND  1617

1618  AERVLRESTSVLTLSMREDVPIRFQRRKD*  1707

 

>CYP666B1 Neosartorya fischeri

93% to CYP666B1 Aspergillus fumigatus = ortholog

NFIA_114140

MIWSSVLLGLAGLYAFDYFRRLFKNIRLAQRTGLPYTVLPFAGNSLQVKILLSIRWLPYIINHWLPDWLADIINDDTYDY

RWTVKDRRVKKLGKMYMVVTAENIVCHIADAHLVTQICNARQSFPKPIWQYGFLNLYGPNLLTCEDQGWAHHRRHTAPTF

NEKNSALVWEESIRQMTEMLHHWQIAGTSEKLHGFVVASTRGDLLKFSLNVLSGAGFGVKLPFKQLPQESSNDPNDVFKD

TAKPPEGFSFSFRSAVAYMNLRIMTVVLATMVIPKWIPRALIPWLKSDFEAHRDLEAYLRKLISIGKAEKTADSSQNLIQ

GLLLSRNKVAANGSSKGTGLTDLEIIGNMHIFTIAGHETTATSLRFTLLLLALHPDVQDWLYEGILEAAHGEPEDVAQWD

YHRMLPKLITPLCVMLEVLRLYPPVVTVPKSTSKTPSPLNYQGKQYLLPPRVNVNLNANCLHYSEQYWGPDVATFYPQRW

DARNQNSFLAKNAGAFIPFSDGFRACLGKKFAQVEFIAALSALSRDYKVELADDSPEGRKDAERVLRESTSVLTLSMRED

VPLRFRRRKD*

 

>CYP666B2 Aspergillus niger

fgenesh1_pg.C_scaffold_12000110|Aspni1

59% to CYP666B1

MIAYTLVLGFIGLVVFDYIWNLAQNIRKAQRTKLPYVASPYNVSIIFIILFGWLLPLLAEYCLPQWMGDLLYDNVGTYRW

TVRNRQVKRYGKVYILVNPRGVFCSVADASVVCQIVNARQDFPKPNNALVWEESIQQTQDMIAQWAEASPLDSTRGSGFT

VESTRDDIHKLTLNVLSGAGFGVKAPFKLLPQESLKNPEDIFRDSPTPPTGFDFTFRSVVAYMNLHIPTMALANNMLPKW

VPRPLVPFFKQDFAAHRDLDKYLQRLITTTESRLSGEENPSNLIEGMLISRKPGDAKDAGLSDREIISNMHLFTIAGHET

TATTLRFALVLLALHQNVQDWVHNGIAEATKDKLPNIEDWNYDDAFPKFVTPLCVMLETMRLYPPVITIPKFTGDSPSVI

HYDGKDYVLEPGTYINLNTNGLHYSEEYWGDDATLFQPQRWDARNKGSFLARNTDLPGLAGPGLEYPTIHKPVRGAYIPF

SDGFRACLGKKFSQVEFVAALTTVLRQYRIELADSSEKGRINAERVLNQSTSIITLAMGEEVPLLFRRR*

 

>CYP666B3 Aspergillus clavatus

71% to CYP666B1

ACLA_066120

MIISSLILGLLGLYAVDYFRRLLKNIRLARRTKLPYIVLPVSGSASIPLKILCGFKWFADAINYHLPGWLADIVNDATYD

FRWTVKDRRAKELGNMYLIVTAEKIDCNVTDASVVTQICNARQSFPKPIWQYKILDLYGPNLVTCEDQAWAHHRRHTAPP

FNEKNNALVWQESIRQTTEMIDHWQRLHSPDDKQGFVVANTRDDFLNLTLNVLSGAGFGVRLPFKPSLQNPIDGPDDIFQ

DTAIPPKGFGFTFRSVMAYMNVNIPTVVLANIVLPKWIPRALVPWLKPDFEAHRDLKAYLQRLIATAAPEKTANSSPNLI

KGLLLSRNQDAGSSTTKDSGLTDLEIIGNMHIFTIAGHETTATSLRFAFLLLALHQDVQDWLYESILVAAQGEPADVAKW

DYSRMFPQLIAPLCIML ETMRLYPPVVTVPKSTSENPYPLEYHGKEYILPPFTNINLNSSSLHYLEEYWGPDAAQFYPQR

WDARNQESFLAKNTLTPGLAGPGLEYATVHKPTRGAFIPFSDGFRACLGKKFAQVEFVVALAVLFRDYKVELADDGPGAR

LDAERVLRESVAVLTLAMRENIPLRFRRRSHCDGL*

 

>CYP666B4 Aspergillus terreus

59% to CYP666B1

ATEG_07596.1

MNNFRWRIKNRQVKRYGGVYLVVTSKTVTCCVADAAVVSQIVNARNEFPKPIWQY (1)

KVIELYGPNLVT (0)

CEDKEWAHHRRHTATTFN

ERNNELVWKESIRQATEMLHYWRQTASPRPDTLALNDISEDIVQFSLNVISGAGFGVQIP

FKPSLSRANSPNDADIFQDTACPPPGFDFTFRSVVAYTDVKIRTVVFANLMLPRWLRRPL

APFLRRAFAAHRDLENYMKRLIETGSASFAGAHGTKETASNLIHGMLASRADAADKAKGL

SDREIISNMHIFTLAGHGTTETSLKYAFVLLALSPRVQEWLRQGILDAVGNEPADPAQWD

YAAVFPKLVTPLCVM (0)

LETMRLYPPVVTVPKWTGASPSRIHYRGRDVVLDPGVSINLNMNGLHYSEEYWGVDADTF

DPARWDARNSGSFLAQYANTPGLVAPGLEYPTVHRPVRGAYIPFSDGFRACLGKKFAQVE

FVAALAVVFRVYRVRLADESDAGRRQAERALQESVSVVTLGMVEGVPLVFERV*

 

>CYP667A1 Aspergillus nidulans AN3272.1 29% to 589A1, 534 clan?

MLSLPSSVFTILVGLFLYKIIRLAHNVRLARKTSLPYIITPLLETEVVAL

LLNPLLRYLYRDYLDRGKGWPRWCRFFVKDWAWEDKRQAHDELGDVFLCV

SPEGIICYSADATMGWDVMNRRNTFTKPRDKYKVLEPYGMNVATAEGKTY

QFHVRITAPPFGDLSGVNNLVWRETVHQTKRLTEAWAESPPTEIQKDVNA

LTLAIISLAGFGKRLEWSKNGEKEGVPAGYQLSFLHALQDTLHYMVPILL

FPRWVLRVILHKAALAHAQLDRYLREIIRDQKAKLSADINHSDKQSRGNL

LTAVVRASMVFDSEQSSKESSRSSGERKQGFTEDETMGNLFIYLLAGYET

TANAIIYGLAVLALHQDIQDQVIEEIDRAHARAQSANRSELTYEDDFEFL

EYTYGFMYETFRLFPGVTLITKMIHQPERIITDGPDGSPKSYDLPAGTRV

YLNAPVVHYHPKYWPEPYKLDPNRWRSSQGEKHVVASDRTRQMKGTLLTF

SDGSRACLGRKFAQAEYVAFFATLLRQYRVRLAPGSDPAVVERDLFGKSA

GTITLAPLANVRLHIQPR*

 

>CYP667A2 Aspergillus clavatus NRRL 1

AAKD02000032.1

91% to 667A1, one in frame stop codon

ACLA_063860

178399  MLPLLSSAFTILAALFLYKVIRLAHNVRLARKTGLPYTITPLLETEVLALLFNPLLRYLY  178220

178219  RGYLDRGKGWPRWCRFFVKDWAWEDKRQAHDDLGDLFLCVSPEGIICYSADATMGRDVLS  178040

178039  RRNTFTKPRDKY (1)  178004

177920  KILEPYGMNVATAEGKTYQFHVRITAPPFGDLSGVNSLVWRETIHQTKRLTEAWAKSPPI  177741

177740  EIQKDVNALTLAIISLAGFGRRLEWSKDGENEGIPAGYQLSFLHALQDTLHFMVPILLFP  177561

177560  RWVLIIILNKAALAHAQLDRYLRDIIRDQRAKLSADINHADKESRGNLLTAVVRASMVFD  177381

177380  SEPPSAKSSRLSGERRQGFTEDETMGNIFIYLLA (1)

177209  GYETTANAIIYGLAVLALHQDIQDQVIEEIDSAHARAQSANRSELTYEDDFEFLEYTYGFMYE  177021

177020  TFRLFPGVTLITKMIHQSERIITQGPDGTPKSYDLPAGTRVYLNAPAVHYHPKY*PEPYK  176841

176840  LDPNRWRSSQGEKHVVASDKTRQMKGTLLTFSDGSRACLGRKFAQAEYVAFFATLLRQYR  176661

176660  VSLAPGSDPAVVERDLFGKSAGTITLAPLGNVRLRIQPR*  176541

 

>CYP667A3   Penicillium citrinum

           BD294641 PAT 04-NOV-2005

           DNAs related to the biosynthesis of ML-236B (compactin)

MAFGPYVAYGALALAAYKIAKLFFFVFQARKTGLPHVLTPFLETEVIAFLLTPILRTVYH

AHLDKETGWPRWCRFIVKDWSWEDKRRAHEEYGDVFLVVSPEGIICYSADAAMGWDVMNR

RKDFTKPRDKYKILEPYGPNVATAEGGTYRFHVRVTASSFNDQSGVNDLVMSETIHQTQQ

LCKLWVKGAAPELQAGVNSLTLAVISLAGFGQRLDVGGKSKKGIKVPQEAKMSFLHAISD

TTKYMVSILLLPGWLLKLTPLRKAHFAHQALDYYLRQLIRDKRKQLVQDINHQDGTSNRG

NLLTAVLRASLTDGDLAKAGGARKDAFTEEETMGNLFIYLLAGYETTANAIMYGLFLLAL

HPEIQEKVICEVDRVWAEANASGRESLSYQHDFDKFYESFRLFPGVTLITKIANGDQPVH

VSNSSGVTTTHILSSGIRVYLSAPGVHYHPRYWPEPNKINPQRWMQNFSNESSGKRVVAA

DRSRQMKGTLLTFSDGARACLGRRFAQAEYIAFFAAFLKGHRVKIRPDHDRQTVWRDIYL

KSAGQITLAPLDTTGLVIERR

 

>CYP668A1X # Aspergillus nidulans AN1300.1 poor match 53 clan similar to 548A3

name changed to CYP548G2P

 

>CYP669A1P Aspergillus nidulans AN0008.1 poor match 53 clan

since this is a probable pseudogene and it matches very well

after MDGPSN, the N-terminal my not be real

       WKGHPFTPVVNIVPAFISSLLAFSLSTSQFAKTYRPFKNAPSSLVNHT

       VKFAGSSAVHGSHRVSILSSA

       SSGSVSGSAPNLLVNAV*TMPGLSA

       AAKTDGFSAAIHAMNLVSANLDVMYPDSPG (2)

       MDGPSNRNIRSDWYDLLHPQVSSIFTRDRILHQKRRQLWTKALSST(1)

       SIQLYTRRILSKIYALES

14773  LIATAADEGKPVLINEVMYWFAFDSVGDFAFSEDFGMLRNEGWHDVIWMFRSALALLGPF  14594

14593  SPAIWMPRLAFSYIPGLWRVKWWFNMLEFCDRCMEQRMK (0)

14416  KTPKESNIASWFLKDHKVHRQNAWLSGDTATLVVAGR

       DTNAPTLVHLFYFLARYPEHTEKIW*

       ELVSVNAEDPVALAKLPHLNGVINESMRLLPAVL  14054

14053  TFGSRTTQPEGLTVEGTFIPGGVKISAPRYTIGR  (1)13952

13871  LKSAFAETHEFIPEWWYSRPDLVKDRRAFAPFGV (1)

       GSTSCVGK  13692

13691  SLALAQIRLVTAALVSKYKFQFTPGMGSGEAVEGEMREQLTAQPGHCYLNFIRHVGQE*  13515

 

>CYP669A2P   Talaromyces stipitatus ATCC 10500

             ABAS01000028.1

             frameshift after NIFVY, stop in N-term, pseudogene or seq errors?

             62% to CYP669A1P from MDGPGNNN TO THE END

257826 MFRKEVFAIALGILAHRIVFIH

GE*HLRAPSVVVIHLVLGLLIFTREVTLKHDRYNELLLHAMILPSCYLTSLFLSIAMYGL

FFHRLRHFPGPRLVALTKLWHVWKCRDSRGHLVLEEWHQQYGTFVRT (1)

GPEEITIFHPAAHEAMDG

257309  PGNNNVRLDWYDLLYPHISSIFTRDRDLHNARRKMWEHALSRT (1) 257181

ALLYGYYQRILRKVKTPESLVADQYYRPININELMYWFAFDSMGDFAFSEDIGMMRNKAWQKNIFVY

RSATALLGSLSPAIWIARLGFAFIPGLWRVRDWFQMLAFCDQCMEQRMKKEVENRDIASW

FIDDYVLCEKTNPNQHRWLSGDTATLVIAGS (2)

DTTAPSLSVVFYFLALYPDHAEKVYAELLTVNRTDQNALARLPHLNGTINESMRLLLAVL

TFGYRVTPPEGLTIEGTFIPGSTKICTPRYSIGR (1)

LECAYEQPNDFIPERWYRRCELIKDKRAFAPFGV (1)

GRTACVGKHLAMVQIPSVVTSLVSQYRIEFAPGENNGEAVERDMKDQLTARPGKFHLLFMPR*

 

>CYP669A3 Aspergillus niger

e_gw1.4.1307.1|Aspni1

65% to CYP669A1P

MSTAIRQYHHRIIRKVEKLVNLIQAEGHRPILINEVMYWFAFDSMGDFAFSMDFKMMENQEWHQVILLFRSALALLGPLS

PAIWIPRLAFAFAPGLLWAKWWFQMLAFCDQCMERRMKQTLKDKDIASFFIEDHSTSTDKRRKMWLSGDTATLVVAGMGL

SKLSHLNGAINETMRLLPAVLTFGTRVTPPEGLTIADTFIPGGTKICAPRYTIGRLESAYERPKEFIPERWYSQEELIRD

RRAFAPFGVGNTSCVGKNLALTQIRLVAAALVIRYRISFAARNPDGERVEGEMKDQLTAQPGPCWLEFSPRDD*

 

>CYP669B1 AAGI01000217.1 Phaeosphaeria nodorum SN15

46% TO CYP669A2P, 45% to CYP669A1P

221162  MAIVPFCLGVIMHLGIFIRGEWHLQAPHIVLGHVLVFTLLLVKQLVSGISTWYGVALPFVV  221344

221345  YLCGLLGSMAVYRLFFHRLRSFPGPRLAVLSKLWHVWLCRDSKNHLVLESWRQKYGT  221515

221516  FVRT (1)

        GPSEITIFHPAGLEWLDGPQNRNARSDWYDLLHPSV  221692

221693  QVFSRDPAIQVARRKVWDKAMSLS (1)  221764

AVKQYYQRIKNHVLELESIISAQNSKPIIMNDLLYRMTFNVLKDIGFGQANEEVEDGAS

KLGGSLTIIGPTNPAPWILRMAFALFPGVWNIPHWFKFLEMTQGI (0)

PESVDIASFFLEDAQRKEADMISREAVGGDCGVILLAGS (2)

TSGPSLILLFYCLARWPEHAEKIRQELRNVDYNDMAALSALPHLTATINESLRLYPA

GPTFGSRVTGPEGLNCDGVFIPGGTKVVAPRWSTGR (1)

LEEAYEAAHEFIPERWYSKPELVKDKRAFAPFSM (1)

GRNSCGGKKVAMSQMRLAVAVLVSKYSIRFAPGTTDEMSVENDMRDQLTPLPGDLKLVFEKLE*

 

>CYP670A1 Aspergillus nidulans AN7066.1 29% to 67A1 34% to 628A1 53 clan

MDDFKIYYYASAGAGIAGILSHLVYFIRGEHHQYAHRWITRALAGTAAVA

IATLRLTDWQPLLSLILTALISTSYFLGLYSSIGIYRVFFHPLRRFKGPF

WARASNLYHMYIIRKSDNYLVMKKMHEKYGPIIRTGPANLSVNDPAAIPL

VLSDRATCVKGPWYDRSLPLVNLHTVRDKRVHDARRKVFTKAFTPSALRE

YEERVAVHCEEFVRQMTRLSGKPFDASEWFKYFAFDVMGDLGLGREFHMM

TSETNRWIPTLLETSMAHVGPTSPVPWMAPILHNLPWAGRGARAWLEFVG

SQVKERTQKKSDRRDQILSHLVEAYNQSEKKNIDYQWLRGDTRLTIVGGS

DTTAATLTFLFYHLAQNPSHVDKLRAELEPLLNGQPRLDPKDVSKAQHLN

GVIQETLRLHPAIPSGFPRTTPPEGITINGTYIPGGTTIVIPVYALQHDE

ANYTHAEEFIPERWYSRPELIKNRDAFLTWNIGTNGCIGRALALTEMRNL

VTYFIHHFSRVKFAHGEDGKALLTETKDHFTVGVKPLRLIFEK*

 

>CYP670A2 Coccidioides immitis

85% to CYP670A1

CIMG_04862.2

MDCTFMTPCAVAGGVGVLSHLLYFIRGEHHKYAHRWITRIFAGITVLAVSVLRVTEYQFLRTLILTILLSTSYFVGLYGS

IGIYRIFFHPLRKFKGPFWARASNLYHMCMIRKSDNYLVMKKLHKQYGPIIRTGPANLSINDPAAIPIVLSDRAKCFKGP

WYDRSLPLVNLHTVRDKKVHDARRKVFSKAFTPTALREYEERVVVHCEEFVRQMSRLSGKPFDASEWFKYFAFDVMGDLG

LGKEFHMMTTETNRWIPTLLETSMKHVGPTSPVPWMAPILHKMPWAGKGARAWLEFVGSQVKQRTQKKSDKRDILSHLID

AYDQTEKKDIDYQWLRGDTRLTIVGGSDTTAATLTFLFYHLAKDPSQVEKLRAELEPLLNGKPQLDPKDVSKAQHLNGVI

QETLRLHPAIPSGFPRVTPPEGVTISGTYIPGGTTIVIPLYAMQHDEANYANAEEFIPERWYSKPELIKNRDAFLTWNIG

TNGCIGRALALTEMRNLITYFIRNFSTVRFAPGEDGTKLVTETKDHFTVGVKPLHLIFE

 

>CYP670B1X # Aspergillus nidulans

          Renamed CYP5076A1

 

>CYP671A1 revised Aspergillus nidulans AN8338.2 AACD01000150

MGIVTALATFLSPDLTRTAFAFCVLGVASHQCIRHGEIDNRLRPLSALYL

LAFLGLCLCYVRIFSLSWPRALGETILAASSFNLGLLTSIITYRAFFHRL

RHFPGPWMAKVTRISAVLKAVERTQYHLDLKE   MHRKYGDFVRTGPREISV

NRPSAVYLTSGPHSVCTKPTWYSHVSDDITKVSLNSTRDPEVHRRRRRAW

DRGFSMKALPTYEPRLQHKVDVLVSQIRSRIDRPLNITQWTMYLAFDVMG

LVGFSKDFRQLEDAVEHAAIEELHGQMLMYGILRPVPWVLTILGATLGLA

GKYGQFMTYCVARTAERKAEWNASEDKVPQDVISWLFKASDEKDQSAPPG

EQALNEDGQLLIITGSDTTSGALANAFYYLAKHPAVYKKLQAELDNAFSS

GKQSPKINNETLRKLPYLEAIINETLRLKPAVPSGQPRQTPPQGLQIDEV

WMPGDTIIIVPQYVIQRDDRYFPSGDEFIPERWLDEKDSLIKHEEAFFPFQL (1)

GRYGCVGKQLALMEMRLVIARIAMEFDLAFAPGETGEAFDRDAKDTFTFN

IGPLMLDFKPRSA*

 

>CYP671B1 revised Aspergillus nidulans AN6101.2 AACD01000104

195432 MNSLLFREALQHASSTDISKLLLSAATLGVLSHVSLFRSLPVEEYLYGLLGLYTATVVAVTVLYLTAT  195635

195636  AFSP LQVLLRVGCISAAFNTGLASSIAIYRLFFHRLRRFPGPRLSKLSRFYDAYLAGKGL  195815

QYHVEIAEMHKRYGDFFRT ()

GPREICIVRKSAVPLLLSPQSKCGKSTFYAQAQTEAEYC

NVHQTRDFEDHRRRRKAWDRGLSVKALATYEPSIRAKADLLVLHIEKNRG

RAIDATKWSMFLSFDIMGKVGFGKEFNNLSTGVEHPGIKAIHDHMAILGV

MCHVPWLLNLISNLPGASFSMAEFFKWCEDEIVQKHRSWDIKETPQDITS

WLLKAYVHKEVSAAPTANALHEDSRAVIVAGSETTATTLASILYFLCKNP

EILAKLQLLLDEAMPGGSSEWTYDKIKTISYLDDIINETLRLRPAILTGG

YRVTPAEGLQVDEVYIPGDVNVFVPTQLIQTDERYYVDAKRFVPERWNEK

KEMIQDGAPYFPFLYGPYVCPKKNLALMSLRISVSKLAQLYDIHFAPGEN

GELFETRTLDTFTTTLPPLHVQFLSR*

 

>CYP671B2P Aspergillus oryzae RIB40

AP007157.1f revised genomic DNA, SC023

join(618168..618393,618483..618641,618711..619251,

619318..619721,619797..620050)

sixth P450 out of 8 on this accession, one stop codon

55% to CYP671B1

VLSPRYAFGLAVDETSVYPDATQQ  possible intron remove after TKRK

MDFISFLHQENFKEVLETDLLQLGVS

AALLGVLLHITIFRTSFCVENHIYNLLGLYATVVLFLFYAYFTITVLSL

MQALGRVALLTTLFNTSLNTSISIYRLFVHRLHPFPGPFACKLT

RFYSAFLAAKNIQYNVELKRLHKQYGDFVRT (1)

GPR*ISILRNTALPLSYGPQTGCRRSTWYGHVDVDDTK

ICMALSHDFNDHRRRRRAWDRAFSIISLSVYEPRVIAQAKKLMAQVEANQGKPLDATT

WSMLFTFDIMGDIGFG

KNFGNLTTGKAHPAISAIRDNMRVIAVVSHLPWLLNMLGKIPG

AAAGYQGFFKWCTDQVETKRK (0)

SWDHDEYPQDII

SWILKAFIDNDVSAPPSEPALHDDSRVVVIAGSDTTALALASIIYFLAKHPQILQKLQ

AELDNAMPNGPRSWTYDKAKTICYIDDIIHESLRLRPSVSGGGYRVTPAEGLQIDEVF

IPGDVNVFVPQQLIQTDERYYKFSKEFIPERWGEKKVEWGTDKAPYFLFSL

GIYGCVGKNLAMLSLRV

AVSTLAQRYDIRFRTGDILER

CIGYIHHFSAAFASCVSPKEIVNTTPFLRLLQVFFILHISGVKVRT

 

>CYP671B2P Aspergillus flavus

98% to CYP671B2 Aspergillus oryzae

stop codon is conserved in both

AFL2G_04121

MDFISFLHQENFKEVLETDLLQLGVSAALLGVLLHITIFRTSFCVENHIYNLLGLYATVVLFLFYAYFTITVLSLMQVLG

RVALLTTLFNTSLNTSISIYRLFVHRLHPFPGPFACKPTRFYSAFLAAKNIQYNVELKRLHKQYGNFVRT

GPR*ISILRNTALPLIYGPQTGCRRSTWYGHVDVDDTKICMALSRDFNDHRRRRRAWDRAFSIK (1)

SLSVYEPRVIAQAKKLMAQVEANQGKPLDATTWSMLFTFDIMGDIGFG

KNFGNLTTGKAHPAISAIRDHMRVIAVVSHLPWLLNMLGKIPG

AAAGYQGFFKWCTDQVETKRK

SWDHDKYPQDIISWILKAFIDNDVSAPPSEPALHDDS

RVVVIAGSDTTALALASIIYFLAKHPQILQKLQAELDNAMPNGPRSWTYDKAKTICYIDDIIHESLRLRPSVSGGGYRVT

PAEGLQIDEVFIPGDVNMFVPQQLIQTDERYYKFSKEFIPERWGEKKVEWGTDKAPYFLFSL

GIYGCVGKNLAMLSLRV

AVSTLAQRYDIRFRTGDILER

CIGYIHHFSAAFASCVSPKEIVNTTPFLRLLQVFFILHISGVKVRT

 

>CYP671B3 Aspergillus niger

fgenesh1_pg.C_scaffold_9000394|Aspni1

65% to CYP671B1

MQLSSSVVSLADIRAVLESDLLSLAASAAGFGLLSHVSLFRTNFPVEDYLFILLALYAVAVLSTAYAYLTLTQFSVAQAL

LRVILISSAFNTGLISSIGIYRLFFHRLHHFPGPVGAKISRFYNAYLAGKDLQYNVEIARLHHEYGDFIRTGPREVCIVR

KSAIPLLFGPQSKCNKSTWYAHVSTNPKYCSVHCTRDHDDHRKRRKAWDQGFSIKALGIYEPRVKSKADLLASHVAKNLG

QPIDVTAWTMFLSFDIMGDVGFGKDFHNLTTGVEHPAIKGVHDHMAAGATLLQVPWLLNILCRIPGAVSGYSSFFKWCAD

EIERKQKTWDANAYPEDVVSWLLKAYVERSVSAAPSRMALNEDSRAVIIAGSETTATTLATVLYYLAKHPSVLAKLHQKL

DEAMPGGADDWGYDKVKDITFIDDIINETLRLRPAVMTGGYRVTPAEGLQVDEVHIPGDTIVFVPVQLIQTDERYYESAK

EFIPERWSEKRYEMKTDGAPFIPFLTGPYICPGKNLAMISLRTSISTIAQQYNFSFAPGETGEAFETGAQDTFTTSLPPL

QLAFQRRQK*

 

>CYP671C1 Nectria haematococca

fgenesh1_pg.scaffold_25000016

Necha1/scaffold_25:46690-48703

45% to CYP671A1, 43% to 671B1 A. nidulans in CYP53 clan

Nterm 46% to CF878446.1 tric078xn06.b1 T.reesei mycelial culture

VGPREISITRE may be an intron seq, or AP007157.1f is short.

Note AP007157.1f does have this seq with one stop codon

Remove two introns from N-term in the model and IENKNK after AKRA

MATLGLVLSAALDSAPLVTAEVSFLLGIVAHLVIRPFEIDSQAWAIVFAYLG

VITTLLLTYIQVCKF TIIGAILRTALVSNAFNVGLAS

SILIYRAFFHRLHRFPGPFPAKLSRFYAMKNAAKALKANEEVQKLHEIYGDFVRV (1)

GPREISITRESAISIIYEPPNQCVRSTWYSQVSDDVRKISLHTTRDLTAHKLRKKAWVRGLGFR (1)

ALAIYEEKIASKVEILMSQIAEKQGTPMDMTLYAAFFGFDVMGQV (1)

GFSKDFNMLDSGYKHPAIQALHENMVAVGVLSTVPWLMSMLGKIPGATGSYARFADWCAREFQAKRA (0)

EILKDHDPRDVISWLLRAEDENDRSAPPGEGAFQEDSRLMIVAGS (2)

DTTAVALTNA (2)

LFFLTKSPTCYRKLQAAVQAQFPEGVKDWTYEKIKLIPYLEYVIHETLRLKPSAPGGLSRL

TPPQGLQIDEVFIPGDTIVSVPTYTIQRDERYWENALEFRPERWENLNPEKVAWIPFSRGQYSCPGKNLA

FMELRMVISRIALEYSLAFPPGDDGEAFDKGARDTFTLNVPELPIIFTPID*

 

>CYP671D1 T.reesei

CF878446.1 tric078xn06.b1 T.reesei mycelial culture

CB906402.1

EST 46% to 671C1 (N-term to C-helix)

FSIIYADSCAPTVGGFLVNHKHLLEVLK

MEEALEFFIIDLSSSWRVTTCVSLLAGIASHHLVFRPYEIDTYIWQLIFIYIASMATVFA

TYIHKRGYQVIPASLWTFFIASVYNCSVIASILVYRALFHPLRHFPGPFLAGLSRFYAMG

RMIHSRKGFEDIQGLHQEYGDIVRVGPQELSINRLSVIRAIYGAHAQTTRPPWYAQMSRH

AAXSSLVNTRDVAMHKMRKKVWERALG

 

>CYP671E1 Aspergillus niger

gw1.5.1493.1|Aspni1

43% to 671C1

WLDVALLTGLFGNIVVYRLFFHRLRNMPGPIAARVSRHYAAYHTIKDAQMHYTVQRLHQQYGDVVRIGPREVSVCRASAI

RAIYGPPSRCIKGPWYDQVTNENDKKPLFTIRDPRVHSKRRRQWDQAAKGINHYHNALQKQSRILIEKIREHQGRPMDVT

NWINCYTFDVMGHIVLGAELGMLKTGKKIPELQVIDEGQRYIAVAGTMPWFPPLMLGIPGLSAILNPFRHHCHKLFDAKR

AAMTKGSEPKDIISWLIQAQDNGDPGAPPTDQAIKDDAWFIIAAGSDTTASALINAVYYLATHPGAQAQLQREIDERLPE

GIEGLSYENVKDLPYLTAVINETLRLKPSVLDGLVRVTPPQGLKVDEDLHLPGDVVVSVPTFAIQRDGRYWEDADQFRPE

RWASIENPANMPFIPFSRGSYDCVGKSIAWMEMRMALAMLVGEYHIQLSDEEQRAFDGKELDNFAMSVPALWATFRPR

 

>CYP672A1 Aspergillus nidulans AN6434.1 31% to 567B1 53 clan

MSLVMAVPHAFSVSDFGDIPTVLVSALSFILVSVWIKTSIDISQLETKLG

FLSGRSAYKFNSLHKKYGKIVRFAPNQLSFNTIKGMRNIYGAGLGKSNTF

LKTGFYKAISRRNIFTATDPYYHATVRKLFSPSLSPGCMAAHEPVIKGCI

ARFHALVKQRMQSQKAISLNDLYYSLSVDMVSEVLLGKSLGCIERGRPFF

WTEQLPRIFYWATIRDQFKGSGVPTLIKWMLRQILRKGIRQRSEEARMRL

IREQLKATHTRRDVMVEVLERADSSTLPEDEIAENFSAIMLAGFHTTSHA

ICATIWLVLSHPETRERLQNELRTAFQSTDEISLDVLAKLPWLNAVITEA

LRVYPPVPLGGARISPGAYVDGFVAFLTGPQVEVSTSLYALHHNGEYFKN

PYKFAPERWVNPESTDRKEAVQPFLLGSRSCIAKYFAQQMLLLTLASFFV

EFDGQYVGKVKDWTTESRCYAFWELPELKVQLQERFASAEVSE*

 

>CYP672A2  Aspergillus fumigatus Af293

          GenEMBL XP_751418.1 also EAL89380.1

          67% to 672A1

MVLVAMSLPSGPFPELAYPVIPFPVLAVFLVPCLVLLAIRLAPKGPVIKGVPGSFVDLYLCG (0)

IGTKLAFLSGRSTYRFHSLHQSYGNIVRFAPGQATTNTVKALR

QIYSTGTGKGAAFLKTSFYRNISRRNIFTAADPVYHASVRKLFGPSFTPGSMHAHAGVIRECILRLHEVI

RDKLETHNTISLNELLYCHSVDTVSEVLLGKPLGCLKRGKPYFWTEQLPRIFYWATIRDQFEGSGIPTAI

KWLLRRFLRKGIRLRAEQARMRLINEQLHAPHTRRDIMVEVMERSGGSELPEDEIAENFSAIMLAGFHTT

QNALCAVIYLVLTHPEAHVKLVQELQTAFASADDISGDVAAQLPYLNAVITEALRLYPPVPLGGPRVSPG

AYVDGVYIPAGTEICTSLFALHHNPEYFSSPYEFLPERWTEPGSTDRKEAVQPFLIGSRACIAKYFAKQM

LQLTLAGFFLEFEAEHVGKVRDWQRQSRCYAFWDVPDLQVKLRKRLID

 

>CYP672A2 Neosartorya fischeri

94% to CYP672A2 Aspergillus fumigatus = ortholog

NFIA_102170

MVLVAMSLPSGPFPELAYPVVPFPILAVLLVPCLVLLAIRLAPKGPVIKGVPGSFVDLYLCG (0)

IGTKLAFISGRSAYHFHS

LHLSYGNTVRFAPGQATTNTVKALRQIYSTGTGKGAAFLKTGFYRNISRRNVFTAADPVYHASVRKLFGPSFTPGSMHVH

AGVIRECILRLHEVIRGKLETQNTISLNELLYCHSVDTVSEVLLGKPLGCLKRGKPYFWTEQLPRIFYWATIRDQFEGSG

IPTAIKWLLRRFLRKGIRLRAEQARMRLINEQLRASHTRRDIMVEVMERSGGSDLPEDEVAENFSAIMLAGFHTTQNALC

AVIYLVLTHPEAHVKLVQELQAAFASADDISGDVAAQLPYLNAVITEALRLYPPVPLGGPRVSPGAYVDGVYIPAGTEIC

TSLFALHHNPEYFSCPYEFIPERWTEPGSTDRKEAVQPFLVGSRACIAKYFAKQMLQLTLAGFFLEFEAEYVGKVKDWQR

QSRCYAFWEVPDLKVKLRKRLVN*

 

>CYP672A3 Aspergillus niger

fgenesh1_pg.C_scaffold_20000080|Aspni1

75% to CYP672A2

MVFAMALPSGQMPEINPSNLSLPVMAVVIIATKFNFVTGRSAHYFNSLHVSYGKVVRFAPGQATTNTIKGLRNIYGAGSG

KGSTFLKTGFYKGISRRNIFTASDPVYHASVRKLFNPSMTPSSMQAHANVVLECTSRLHDVINATLKSTDTISLNRLLYC

HSVDTVSEVLLGKSLGCLKRGKPYFWTEQLSRIFFWATIRDQFAGSGVPTMIKMMLRYLIRKGVRSRAEQARMRLINEQL

TASHNRRDIMVEVMERAATSDLPVDEIAENFSAIMLAGFHTTQNALCATIYFVLTHPEAHAKLISELQTAFASPEDVSGD

VAANLPYLNAIITESLRLYPPVPLGGPRVSPGAYVDGTYIPAGTEICTSLFALHHNPEYFNEPYEFIPERWTDPESTDKK

EAVQPFLVGSRSCVAKYFAKQMMQLTLASFFLEYEAEYIGPVKDWQRESRCYAFWEVPDLKLKLRKREAI*

 

>CYP672A4 Aspergillus oryzae

Supercontig 18: 1723786-1724196 (-) strand

MVFVALYLPSGQMPEGYSPAMISYPTLVVVL (0)

FIFLVILFVQVQPKGPTIKGVQGPFLDIFCCG (0)

IATKLAFISGRSAHHFHALHESY (1)

GKIARFAPGQATTNTIKALRNIYGSGTGKGSAFLKTGFYKSISRRNIFTASDPIYHSSVRKLFGPSMTPGSMEA

HAGVVRECTLRLHDVINSRLESKTILSLNELLYCHSVDTVSEVLLGKPLGCLKRGKPYFWTAQLPRIFYWATIRDQFDGS

GVPTVMKWLLRRFLRKGIRLRSEEARMRLIHE (2)

QLKASHTRRDIMVEVMERAETSGLPESEIAENFSAIMLAGFHTTQNAL

CAVIFFVLTHPESHAKLVRELQGAFHSAEDISGDVVQQLPYLNAVITEALRLYPPVPLGGPRVSPGAYVDGVYIPAG (0)

TEICTSLFALHHNPEYFNEPYEFIPERWTEHGSRDKKEAVQPFLVGSRACIAKYFAKQMMQITLAGFFLDYEAEYVGTVKDWQ

RESRCYAFWELPDLKVKLHQRRQA*

 

>CYP672A4 Aspergillus flavus

79% to CYP672A2 Neosartorya fischeri

99% to CYP672A4 Aspergillus oryzae

AFL2G_10674

MVFVALYLPSGQMPEGYSPAMISYPTLVVVLFIFLVILFVQVQPKGPTIKGVQGPFLDIFCCGIATKLAFISGRSAHHFH

ALHESYGKIARFAPGQATTNTIKALRNIYGSGTGKGSAFLKTGFYKSISRRNIFTASDPIYHSSVRKLFGPSMTPGSMEA

HAGVVRECTLRLHDVINSRLESKTILSLNELLYCHSVDTVSEVLLGKPLGCLKRGKPYFWTAQLPRIFYWATIRDQFDGS

GVPTVMKWLLRRFLRKGIRLRSEEARMRLIHEQLKASHTRRDIMVEVMERAETSGLPESEIAENFSAIMLAGFHTTQNAL

CAVIFFVLTHPESHAKLVRELQGAFHSAEDISRDVVQQLPYLNAVITEALRLYPPVPLGGPRVSPGAYVDGVYIPAGTEI

CTSLFALHHNPEYFNEPYEFIPERWTEHGSRDKKEAVQPFLVGSRACIAKYFAKQMMQITLAGFFLDYEAEYVGTVKDWQ

RESRCYAFWELPDLKVKLHQRRQA*

 

>CYP673A1 Aspergillus nidulans AN5433.1 29% to 57a1 53 clan

revised frameshift after SGKR

MALLESAVVLQVFRDVTPMAILATLLLTTFLTYVVYMRHFHPLSQCPGPF

FASLSNTYKAYYVYKLTIHEKLLELHNTYGPIVRVGPNHLHTWKGDAIAL

IYKGGRSMGKSEFYDAFTAFRPNLFGGRDEDIHALRRRQLSHGFAQVSVA

KLEPLIDGQMAILISKLRHYARTGQAFDLKHALNLYVLDILGEVAFAKAF

DVQNTEDNEKLHAINDHLLLAGVIGELPCQNFTKFLSRLSPVPWMRRLMK

SRNKLKEICAGCVRFKIENLEATKSRPDLLRSLVEATDPESGKR

KAEEEINSEAFAVL (2)

VAGSHSTAGTLTLLFWHLIQNPSIMRKVQAEIENTLGPLKDRTSYPIAGIESTLKY

TMACVRENFRINPVFTMPLWRRVGKSHVLEIDGHHIPEGTNICISNYVLH

HNPSVFGPDHNTFVPEKWLDESYNREKGRYLIPFSVGHRMCIGRNLAMTN

ILKSVCTLATLFEFEPVEKKKDVRVISPGIGEMKGGFEVRVRVREVKRGS

YKNALST*

 

>CYP673B1 Nectria haematococca

e_gw1.10.303.1

Necha1/scaffold_10:98331-99989

MDFDRLLAITSDHRFVVVTALIALFTGLYVFYYRNLHPLSKYPGPFLASFTNLWKVQQLWSLHLPDTLIA

LHEKHGDVVRIGPNQLSFRQGDAVPKIYKAGRTLAKTPFYDGFTSFNPNLFGTQDEE (0)

VHSLRRRQMAHAFSLQSIKEMEQYIDEHLVQFRRNLDEYSKTGQVFDLKELIAFFVLDVLGDLAFRCQFN

SQVEKDASKLPPINDHIFLACLLGMIPDLMPFIKAISPWTPVPWLQQLLAARRDLKNLTAKCVSSRMADT

GAARKDLITSLINAVDPQTGARLTELDIQTEAFAFI (2)

VAGSHTTSGTLTLLFSHILQNPAIHAKVVGEVDATLGKLESDIVPIE

GLETKLPYLMACLNENFRINSVFTMPLERRVTAREGFEIAGHVIPKG (0)

TVVFSLNHVVHHNPSIWGADHNKFDPSRFLNKDGERLERFLSPFSMGHRMCIGRNMAMTNMLKLVATTFK

NYHLEMEDPTQIISTISVGISEKEGPLMCRVRRRC*

 

>CYP673B2 Fusarium oxysporum

58% to CYP673B1 Nectria haematococca

FOXG_13065

MSLELESFITWASSLPILLQTSAVVGFLGLVYIVYYRYLHPLAK

YPGPPLASVTNLWKTYHLWNLHLPHTLVRLHEQYGDVVRVGPNDLSFRNPDAVNTIYKGG

RQLQKTGFYDGFTTFNPNLFGTQDEE (0)

IHAIRRRQMAHAFSLQSIKEMEHFVDSHILKLRNNLDHFCDSNQDFDLKDMIALYVFDVL

GELAFSRSFDSQDERDLARLPPINDHIYLACLMGMTPDALPWIKKVLPFIPIPWLQRLFN

ARAQLRNLTAACVRQRIEAGSSGRKDLLSCLLVAVDPETGSKLTELDINTEAFAMI (2)

VAGSHTTSGTLTLLFSHLLQNPEVLNKVIQELDSNLSNHTGQVISYQALEKDLPYTRACM

HENFRINSVFTMPLPRKIMTPGGLVIQGCQIPQK (0)

TTVFALNHVVHHNPSAWGKDHDQFIPDRFLGPNSKDLQGYLSPFSTGHRMCIGK

NLAMMNILKVLSTVLRNYKLEMVHPQEPVDTLSVGISEKKGRLMCRISKR*

 

>CYP673B3 Fusarium verticillioides

86% to CYP673B2, possible ortholog

FVEG_12478

MADMSLKIESFTNWAIGLPMLLQTSTVVGFLLLGYIVYYRYLHPLAKYPGPPLASLTNLWKTYNLWNLHLPHTLVRLHEQ

YGEVVRVGPNDLSFRNPDAVNTIYKAGRQLQKTGFYDGFTAFNPNLFGTQDEEIHAIRRRQMAHAFSLQSIKEMEHFVDS

HILKLRKSLDNFCDTNQEFDLKDMIAFYVFDVLGELAFSRSFDSQNERDVSRLPPINDHIYLACLMGMTPEALPWIKKVL

PFIPNRWIKRLLNARAQLRDLTAACVRQRIEAGTSDRKDLLSSLLAAVDSETGSKLTELDINTEAFAMV (2)

VAGSHTTSGTLTLLFSHLLKNTEMLNKVIEELDSNLSSHTGQVIPFAALEKDIPYTMACV

HENFRINPVFTMPLPRKIMTLGGFTIQGQQVPQK (0)

TVVFALNHVVHHNPSVWGKDHDQFIPDRFLGPNGKELQSYLSPFSTGHRMCIGKNLAM

MNILKVLSTVLRNYKLEMLHPEERVETLSVGISEKKGGLMCKMSRR*

 

>CYP673C1  Aspergillus oryzae

          GenEMBL BAE61747.1

          43% to 673B1, 37% to 673A1

MTLLQNIVSETPLAAGSALLTAIIVAYVIYQRYLHPLAKYPGPFLASITDLWQVHQFLSLQQPYNLTALH

ERYGPIVRYGPDKLSITHESAVPTIYQKSAKSMPKTEFYDAYGAAHPNVFGMRDEVMHSVRRRHMSHSFS

LSYIKEMEEYLDLNIRILKDKIRYHSEIGEVFALKKALHYYMIDVLGELAFSRSFGVQEADDESRIPPVI

EHSLLAAVTGAWPTMTMTLKRWLPYMPHAGLRRLFAGRKACADLASSSVQRRLRDLNDGESSVGVQNRKD

ILTNLIKAKHPETGERLTQTDLETEAFGFIIAGTHTTSATSTLLFYHLLHNPDFMRKCTEEIDSNLPSLG

PSETAYSITAAEASLPFLRNCIRENFRITPVFTMPLARRITDPAGVTIEGEHLPQ GTSVAVCNHAFHHNP

VVWGEDHNIFNPNRWNDPNVGAKARLLMHFGLGGRQCIGKAVATTNIYKLLSTLLKEFTFELADEQERVD

VDKGLYKGRIPKLFSVGISDLQGPLLVRARVR

 

>CYP673C1  Aspergillus flavus

99% to CYP673C1  Aspergillus oryzae

AFL2G_09403 revised

MTLLQNIVSETPLAAGSALLTAIVVAYVIYQRYLHPLAKYPGPFLASITDLWQVHQFLSLQQPYNLTALHERYGPIVRYG

PDKLSITHESAVPTIYQKSAKSMPKTEFYDAYGAAHPNVFGMRDEVMHSVRRRHMSHSFSLSYIKEMEEYLDLNIRILKD

KIRYHSEIGEVFDLKKALHYYMIDVLGELAFSRSFGVQEADDESRIPPVIEHSLLAAVTGAWPTMTMTLKRWLPYMPHAG

LRRLFAGRKACADLASSSVQRRLRDLNDGGSSVGVQNRKDILTNLIKAKHPETGERLTQTDLETEAFGFIIAGTHTTSAT

STLLFYHLLHNPDFMRKCTEEIDSNLPSLGPSETAYSITAAEASLPFLRNCIRENFRITPVFTMPLARRITDPAGVTIEG

EHLPQG (0)

TSVAVCNHAFHHNP

VVWGEDHNIFNPNRWNDPNVGAKARLLMHFGLGGRQCIGKAVATTNIYKLLSTLLKEFTFELADEQERVD

VDKGLYKGRIPKLFSVGISDLQGPLLVRARVR*

 

>CYP673C2 Aspergillus niger

e_gw1.1.1529.1|Aspni1

70% to CYP673C1

MTVFIQTITSTPMTSAGLFLALILIVYSIYQRYLHPLAKYPGPFLACLTDLWQVYQFLTLKQPYNLTELHAKYGPIVRYG

PDKLSITHEGAIQAIYQKGGRGMPKTECYDAYGAAHPNVFGMRDEEQHSIRRRHMSHGFSMSYVKEMEQYLDVNIRILRD

KIREHSSHNRAFDLKKALHYYVVDVLGELAFSQSFGIQETDDEARVPPVIEHSLLAAVTGAWPAMTTTLKKYFPLVPYKP

LQQLFAGRKACADLASECVRRRLGDFGGNGMSKSDDRKDILTNLISAKHPDTGKHLTQTDLETEAFGFIIAGTHTTSATT

TLLFYHLLHNPRYMEECVKEIETNLPPLTYGETAYSVSAAEAALPFLRNCIRENFRITPVFTMPLARRVMRPTGVMIDGQ

HIPQGTSIAVCNHAFHHNPDVWGDDHNIFNPYRWENTEIAAKARLLMHFGLGGRQCIGKTIAMTNIYKIMSTLLREFNFE

LADKQAQRNGMPELISVGISDLKEPLMVRAHVRVQEKVEWAHNL*

 

>CYP673D1 Mgr015 Mycosphaerella graminicola

49% to 673A1, 47% to 673B1, 39% to 673C2

MASPVAVLLLLLAFSYIVYQLFLHPLAQIPGPRLAAFTNLWKVYHIYSTQLHEVLLELHKVHGPVIRIGPNDVH

(32 aa gap)

NLFGTQ

DDDLHALRRRQLAHGFSQKSIVAMEDILDERLTVLCDRITMFAETGQPFDLKDCISRYVLDILGEAAFSCSFNAQIPGAG

EFEQVPKAINGHVLMGSIIGELPFQSLNKRLLAGSPFKWMREIVSNRILLRNTCAECVQARLDGGSNKDRKDLLQSLIKA

KDPETGASLTTIDINTEAFAMLVAGSHTTAGTLGLFFAHSLRDPQIMARLVSELESKLDPLDQSTGRQTHAITGLEDSLP

YLMACVRENFRMTPVFTMPLWPRVTASEGLAVWNTVIPSNTNVSASNFVQHHDASVWGADHAKFDPDRWLDGRTEGLSAA

LIPFSLGHRMCIGRNLAMTNILKTISTLLTRFEITMMKDAKKESVRFESYGIGELQGTLMCTARFRNI

 

>CYP674A1 Aspergillus nidulans AN7969.1 32% to 532A 53 clan

MAMSFSYSPADIFWPCVKLGVFLALVYITSLIVYNRYFHPLRHFPGPAWA

AISPLWYFKRIRSGNPQDVQHSLHERFGDIARIGPNILAVRHPDAIETVF

GTKNGKAWRKAAFYDSFDPHIPNARTDSFSERDDAKNAERRRLVGGLYAQ

DNVLRYEPCVDRLIHLFQQRMQEHSESGKVFDMSVWLERYTFDVVGEIFH

GRREGFGMLRDGADYNGWCYLMGVMPDIGAATTYLPWGFRSLYLLSQLVF

QSSRDGVRGMLDVTKQAERATLERWEEMKKGKVLSESDILTGLLDMVQER

GKNDSVMASWTVADVVTEVWAVIWAGSDTTATALTSIFYHLHRNPCKLAG

LREEIDAAFEDGRLRFPIRFNDARKLPYLHAVILESMRVHPSLGIGLPLE

SPADGANIGGTYIPGGVEVIVNPAAVHFDERSFGADAKEWIPERWLVDAE

ATRRMERSMLQFGYGPRMCIGRHISNIEMYKLLPALIRNFEFEMLVDSWE

VKGSWFHRAHNVSADPPPLDKQSFSENGGNLRS*

 

>CYP675A1 Aspergillus nidulans AN4643.2 AACD01000079 revised sequence assembly

MDLIVPNYFKYVNIDTLLLFPLALLVSLAYCIMWITYTRWFHPLAQFPGP

FWASVSRIWTVLHVLPGNAEKTQRKLHEKY (1)

GPIVRIAPDELITSDPAAVKTLYGVKSGTLKTDFYLAFRPPW (1)

ARFPDHFSAIGGKQH

ADRRRIVSAVYSMSSILQSEQYVDACIDLWEEKLGEMADRKESFDLWLWTRM (2)

YAYDVIGELFFSKMFGFLEAGGDHLGYIAATD

DLIPVQFLAGIMPTYVRPLFLLTGFLLPKVRGALTALASLTEATNATIKR

RLFALSELDSNTKPQRADILGKLLDISHKNGKALNFELADIKMESFSGF (2)

FAGSETTALTLSGILYNIFRNRAVYEKLTSEIDAAITSHQLSRPHIT

YTEATRLPYLGACIKEGIRMHPITGVSFPRHAPSCGCEVGGYWIPGNTRV

GVNPAVIHFDKSVFGEDAGIFRPERWIEAGANVANMDCHIMQFGMGARVC

LGKNISMCEIYKAIPQLLHSFTFEMGAEEPVKTTSYWFHKPVAIHVKVRRR*

 

>CYP675A2  Aspergillus oryzae

          GenEMBL BAE55129.1

          63% to 675A1

revised 3/23/2009

MDLLRLRDRSLLGQGALVAFVTYWAVWIIYTRWFHPLAKFPGPFWASVTRAWTLLHVLHGDAEKRQMKLHAKY (1)

GSVVRIAPNELVTSEPEAIQTLYGARSFTAK (0)

TDFYFAFRPPW (1)

ARFPDHFSSEGGKQHGERRRIVSHVYTMTSILQSEKYIEKCIAVWLEKLGQMADRKESFDLWIWTRMYAYD

VIGELYFSKMFGFLQAGHDHLGYIDATEDLVPIQFLAANMPTYVRGLFMLTGILFPKVRRALRALGDLTD

ATNAMLKDRLAAIQSDSEDKPQRHDILGKLLEISQKRGKELDFVLDDIKMESFGAFFAGSETTALTLSGI

LYHILRNRSVYEKLTAEIDTAVQCNQLSTPHISYNDAIKLPYLTACIREGIRMHPITGVSFPRHAPSSGC

TIGGYYIPPNARIGVNPGVMHFNKTVFGEDADQFRPDRWIDGDVSKMDRYIMQFGMGARTCLGKNISMCE

IYKAIPELLKFYTFELAGDEDMQTTSYWLYKPVAIDVNVRRR

 

>CYP675A2  Aspergillus flavus

97% to CYP675A2  Aspergillus oryzae

revised 3/23/2009

MDLLGLRDRSLLGQGALVAFVTYWAVWIIYTRWFHPLAKFPGPFWASVTRAWTLLHVLHGDTEKRQMKLHAKH

GSVVRIAPNELVTSEPEAIQTLYGVRSFTAK (0)

ATLGYVRTQYYPQQRLIGVA

TDFYLAFRPPW (1)

ARFPDHFSSEGGKQHGERRRIVSHVYTMTSILQSEKY

IEKCIAVWLEKLGQMADRKESFDLWIWTRMYAYDVIGELYFSKMFGFLQAGHDHLGYIDATEDLVPVQFLAANMPTYVRG

LFMLTGILFPKVRRALRALGDLTDATNAMLKDRLAAIQSDSEDKPQRHDILGKLLVISQERGKELDFVLDDIKMESFGAF

FAGSETTALTLSGILYHILRNRSVYAKLTAEIDVAVQCNQLSTTHISYNEAIKLPYLTACIREGIRMHPITGVSFPRHAP

SSGCTIGGYYIPPNARIGVNPRVMHFNKTVFGEDADQFRPDRWIDGDVSKMDRYIMQFGMGARTCLGKNISMCEIYKAIP

ELLKSYTFELAGDEDMQTTSYWLYKPVAIDVNVRRR*

 

>CYP675A3 Aspergillus terreus

58% to CYP675A2  Aspergillus oryzae

ATEG_07776.1

MLDNIPDRQLILQAVVLGFATYWAAWICYSRWFHPLSRYPGPFWASVSRVWTVMHVLRGDAEKAQKSLHARY

GRVVRIAPNELAINDPQYIKKIYGINSRFTK (0)

SDFYLSFRAPF (1)

SRYPDHFTSTDEKVHAERRRIVSHVYTMTSILQSENYLDECTAVFLEQMG

KIADRKGTFDLHEWARMYAYDVIGELYFSKMFGFLKSGCDHLGFMASTDTLIPVMTLSAVMPTYIRSVFMFAGILFPRVR

NALTALGNLSKAAETAVQERLAQRDESKDGPERADVLNKVLDIYHGKKADFDLDDVRLEAFGAFFAGSDTTAIFISGTLY

HIIKSRDVYDTLTREIDQGTRNGLLSTPFITYNEAVKLPYLSACIKEGMRVHPSTALTFPRNAPKGGCDIEGHWIPETAR

VGVNAAVVQFDKSIFGDDADTFNPSRWLGPDADNMSRYILQFGAGSRTCMGKHISMAEIYKIIPALLRSYHFELDGNGDL

KTSSYWFYKPANITTRVQRRQV

 

>CYP675B1 Nectria haematococca

gw1.22.151.1

Necha1/scaffold_22:515970-517756

50% to 675A1, 43% to e_gw1.1.1095.1

add cyan region

MILPISAETLKGVSFSFPPLLTCACLFLGLFILYWCF

WIAYTRHLHPLAKYPGPFLASVSRSWLVMQVAGASADKTQRKLHSRL (1)

GPIVRIAPDEVAISDPDAIRSIYSINSGFTK (0)

TDFYLPWRPLW (1)

AKYPDHFTSLDERLHASRRRIVNSLYSMTNIVRAESGIDLCTDLLMSRMREYADTGEVVDISSWVQM (2)

YAFDVIGQLFFSRMFGFLKEGGDHRGYIRSLDTLLPILAVASAMPTYIRTLFSLAGTMFG

SVRGALTAINEIERAAESCIAERQEILNNGETVERKDILSALFEIIQEKGDKVNFGLTEAKVEVYVAL (2)

FAGSDTTAAAISSILYHLVKSPQAYQKLTEEIRQGAASGELSVPHVRHSEAVKLPYLEACCKEGMRMHPSVG

LTLPRNVPKGGGMVCGEWFPEGTRVGVNAAVVHRDKTIFGDDANEFRPERWFRDDAKNMDRYMFQ (0)

FGGGSRTCIGKN (0)

ISLCEMYKMIAQLLLSFDLEDACTEWKTSNYWFNKPSN VNIRVRHRSRFIN*

 

>CYP675C1 Nectria haematococca

e_gw1.27.138.1

Necha1/scaffold_27:47233-48946

46% to 675B1, 42% to 675A1rev A. nidulans

in CYP53 clan, in CYP675 family

This gene model seems correct

MALQIIFDYVLAFPNNLAVILGLAILYWVGYSIYSVFFHHYSDIPGPFWAKVSRLWLAKQVLSGTVDQTQRALHAKY (1)

GSIVRIAPNEVSISDPDAIKTIYAVKNAFSKTDFYTPFAPHLSPNEDLFTQRDEKHHSYRRRF

VNNIYSLKSVLESEPFIDQVTSVFRTRLDEFAVSGQSIDLGLWLQMYAFDVVGELFYGKQFGFLKNSHDY

ENYIDSLDALLPALSTICVLPAYVRPLKVFGSLVPQIRKG KICYDKIVLAAKQVVKERQQ LMMTDK (0)

KVLLTKRCSHF RTDILDKLFQLS ADKEDFSIPDIETEAWVSIFAGSDTTSIATRSILYHLLMTPDALAKLTNEV

DNAVADGTLSDPIKHSEAVKLPYLSACCKEGFRVHPSVGMSMPRHVSEPGATICGRYFAPGYRVGMSAAV

VHWDKSIFGQDADVYNPDRWLRPEGKEMERYLLHFGAGTRTCIGKN (0)

IALAEIYKLIPELVHRYRFKVIEPEKVQKHSFWFFKQTGFNVRVHRREGVKGGLLVQ*

 

>CYP675C2 Fusarium verticillioides

57% to CYP675C1 Nectria haematococca

FVEG_09420, no F. oxysporum ortholog found

MIIDIILERFTCVSAPAALALLVSSTAALWLMTLLYSYYFHPYADIPGPFWAKL

SRFWLVRQVLRGDIHNTQRALHEKYGPIVRMAPDEVSISDPAALKV

IYVVNAGFTKTEFYTPFAPHLSPHEDLFTQRDEKIHAQRRRFVNNLYSLS

SILESESYVDACIMTFKAQLDEFVAQSDPFDLGLWLQMFAFDVIGELFYG

KQFGFMEDRHDYQGYIETLDTLLPAVATSCVLPFYLRPLQVLGHLIPPLH

KALKGYDDIVVAAKETVARRQRQVDKGTVQSRPRLKKYVLADPRI

RSDLLDKLFNIAASKGDFTLADVATEAWVSLFAGSD

TTAIAMRAILFNIIRNPKVYERLLAEIDQASAQGLLSNPAKYSEAIKLP

YFIACCKEGFRVHPSVGMSMPRHVPPAGVNIAGRYFPGGSRVGMSAHVVHF

DKGIFGQDADCYNPDRWLQPGAEVMERYLMHFGAGPRTCVGKNISITEIHK

LIPSLLQDYEFRLAEPNKDLKYSNYWFNKQIGLHVLVSQRKK*

 

>CYP675D1 Fusarium oxysporum

42% to CYP675C1, 42% to 675C2

FOXG_14720 revised

MLLDFAFTNTWLIPASLATYWLGWLVYTLTFHPLSKIPGPYLARLSRLWYLHKIWT

EDVEKHERALHAKHGPLIRIAPDEISCSD (2)

PEAFASIYRFSNALDKGRFYEPYNTTGFSPHGDVFSCKNDKKHGQRRRITSNIYSMTNVASSEEYIDYCSNLLTERLGQ

FADSGKPCDIGEWLHW (2)

YTFDIIGELFYGRAFGFIDEGKDQNDWIKSLDKMIPFVCLMGVAPPILRPLIGLGTMISSAGNEIRKGV (2)

KNIGESSSRLMKEAYASRIEAP

RTDMAQQIFSIFEKNGERMDFRWGDVEQESYGALFAGSDTTAIAFRSLFYHLM

HNPEAYSRLEIEIDRAVDDGLLSMPATYKEAIKLPYLCACIKEALRIWP (1)

GINLILDIALPRTVPPEGLTICGQFIPGGYVVGINAAVMHFDKRVFGQDADSFNPDRW

MDTARANYMDKYMMAFGGGTRTCLGKNIALIELHKLSPQLIRNYRFEFYDRNRTRWHTRNTFFARQEGIVVRVKHREH*

 

>CYP675E1 Mycosphaerella fijiensis

42% to CYP675B1 Nectria haematococca

42% to CYP675C2 Fusarium verticillioides

39% to CYP675A1 Aspergillus nidulans

estExt_Genewise1.C_160602

MFTLPILVLPLLSCVAWIVYTGYVHPLSKVPGPWLAALSRFWYIYHMRKGHFDQVNRQLHAQYAAGPVVRIAPNEVSIDS

PEAAHIIYNNRPGQRFTKVRIVCWVHRKRMSSKALIGLGKHENLFMDLDEDHHASRRRIVANLYSMSSILESESYIDDCT

NLLEDRLTGFYKRGEVVDISRWFQMYAFDALGELVYGEAFGFLESSSDVGGWMALIESLSPFRQTLLLLPAYLRKPYMMS

GLLYKSVKLAITDNAKRVNYARDLVHKRMSELKDNGGAAESQGRRDLLSKMFELSATKGDKVDFHLPEIEMESWVNIQAG

VDTTAIALQAILYYILKDSRVHRKLLSELDAAHQDQRITIPLKYSDALKLPYLAACIKEAMRLHPSVGLQLPRHPPPEGC

HISGYYFPHSARVGVNAAVLHYNQDVFGEDAASFRPERWSADNAAEMDRHLLTFGAGTRTCTGKHISLAETFKMIPHVLL

KFDMKLAEPGMELTKQDWWFHKIKDFRVSIQRELRP*

 

>CYP676A1 Aspergillus nidulans AN8952.1 36% to 629A1 53 clan

revised 2/10/2009

MSSPWKVMDPNLADMHQIASFLGLHRVALFLIAAVIVRVLYRRYWSPIRD

VPGPFWASFSSLWRVYHVVKGHTEQQILKLHKEH (1)

GDFVRIAENEISVAHPDAVKQLLHANLAKGTFYSVFSLPDYRYVN

QMSELDPTRHIQKTRNLSAGFSLSNITKTEPYIDRCLKVLSRQLDGLAAS

DAPVHFQDWFSFFAFDVLGEVTFSKSFGFLQEGLDIRNAIANTGSLVYYI

SIMGNYVWFHNLTLGNPLVSRLGLQPNSHIFDTCLLAIDSRKNNPELRHD

MMQRWLDMRASHPERMSEEDIFGAAVANVGAGAETISSTAQAVIYYLLKN

PQYLATVRKELDEAQAKGELSDVIQYGEATKLPFLQACLKEAYRFHPGVC

HNLPRISPKGGMTIAGRYFPEGVILSVHPWVIHRNADIFGADCDTYNPTR

WLQGDTKRMDYFLIHWGAGYNQCPGRNLAQFELSKVLATVLRDYDIKLMN

PKSEWRFETRFLAVPYGWPCQIQRRKRGMVQVAA*

 

>CYP676A2 Aspergillus niger

gw1.2.1506.1|Aspni1

62% to CYP676A1, revised 2/10/2009

XP_001399153.1

MPVLFASAWLPGSLTSLPVSLPFLLFVFVVAIAVKRRYATSIRDIPGPWLASISSLWQVYQLIKGHTEQEIVKLHRRY (1)

GSFVR

IADNEVSVAHPDAVRLLLHANIAKGSWYSIFSLPDYHYVNQMSELDPQRHIRKSRNVASGYALSNIIKSEPFVDALLGLL

EEQFDKLIASGQPVEFDRWFNYFAFDVVGEVTFSSPFRFLETGTDIRNAIANTRALALYIAIMGHYVWLHNLTLGNPLLS

RIGLQPSSHIFDTCLAAIDSRKKNPDVRLDMMERWLHVRREYPDRMDEAEVFGAAVANIGAGADTTSATLQAFFYYLLRH

PQYLQKLRQEVDEAHTRGDLSHVVSYAEAAKLPYLQACIKETYRYHPATGTGLPRVVPAGGLTIGGRHFTEGTLLSINPW

AFHRNPDLFGPDCDDFNPERWLDPDRARQMDPFLIHWGAGYNQCPGRNLAHFEISKVTATILRDYDIEQVDPKKPWSFET

HFTAVPYGWPCWLRRRQPAA*

 

>CYP676A3 Aspergillus clavatus

73% to CYP676A2, 63% to CYP676A1

ACLA_060010

MGVFALTTGLESPSTPGFWVLAFLSLVATFAVKRKYSTALRDIPGPFLASFSSLWKVYQVWKGHTEEEMIRLHKKH

GYFVRIAENEVSVSHPDAVRQILHAKIVKGPMYAIFSIPDYHYVNQMSELNPRRHIEKTRNVAAGYALSNIIKSEAYVDTLLQL

LKTRLDGLSESGTPVEFDRWFNYFAFDVVGEVTFSKSFGFVESGTDIRNAIANTRALALYIALMNPYVRLHNLTLGNPLL

SRLGIQPSSHIFDTCLAAIDARKNNPDSRNDMMERWLHVRATYPDRMAENEVFAVAVANIGAGADTVSATLQALVYHLLR

SPGHLQRLRAEIDAAKAKGELSAVVQYAEAQRLPFLQACIKEAYRFHSAVGLGLPRVVPKAGMTIAGRHFTEGTILSVNP

WVFHRNPALFGADCDTFNPERWLDKERCAEMDSYLIHWGAGYNQCPGRNLAHFEISKLAATLFRDYEIEQIHPKQEWMFE

NHFTVVPYNWPCRIRRR*

 

>CYP677A1 Aspergillus nidulans AACD01000158.1 FGSC A4 revised

MDAYTSLLALAGALISYSLSYVCYTFYWHPLARFPGPPLAALTRFYRAYI

DISWKHSFVHHLGELHKKY (1)

GDIIRIGPNE (0)

LHFRSPAAYLEIFNPANRWDKEERLYHSFGEDRSSFG

YLRYAEAKERKDILSRRFSRKAVQDAQKIVEGI (0)

VLDLCKTLGQNSEEAVDLFYAFRCMSVDVIT (1)

YLCFANSVNAVHAPKYESPLLLAMDASMTVFPAFKHFGFYKEMIMNC

PPKLSKILSPATAGLVDLQTLLKAQIEDLTSDPSQLEKLPHNTTIYHELLRP

EAYRTKTQPSKGSLYEESQALMFGGADTTGMTLMHGCFYILQEKSRGIYE

RLKTELVEAWPSLDGAAPTWEELEKLPYLTAVIKESLRMSPGVASPLPRV

VPKSGAVITKTHIPGGTIVSQSSHFVHTNPDIFEDPHSFIPERWLGEKGK

SLDKWLLAFSRGPRSCLGQQLAWAELYLTYAHVFRKFDLQIDPSR (2)

SPNELKWKDTFLAHYLGPHLKAKLTPVIS*

 

>CYP677A2 AAEC02000027.1 Coccidioides immitis RS

64% to 677A1

CIMG_06137.2

1175231  MKPFVLTEFGFQSHWILLAALYLAGWAVYQGIK

1175330  TLYWHPLAKFPGPRIGALTRLYKTYVECVAQGSFVHLLEEHHARY (1)

         GDIVRVGPNE (0)

         LHFSNPATYLEIYNPSNRWDK  1175686

1175687  EESLYHSFGEDRSSFGFLTYKEAKERKDVLNRMLSRKAILGSQGIVQEK   (0)1175833

1175898  VVELCKSFERKKNSTVNLFYAFRCMSIDVIT (1)

         YLCFGSSVDAIN  1176077

1176078  APDHQAPIIIAMDASLPVFVRFKHSAFYKSMIVNCPPAISKIVSPLTAGLVDLQQ (0)  1176242

1176296  LLKGQIKEMTEDPSTLEKLPHSRTVFHELLRPEAYKSRTVPCADSLYEEAQALMFGGADT  1176475

1176476  TGTTLMHGTFYILKQPEVYRRLKEEIHAVWPNLNTLPDLTDLEKLPYL (0)

1176476  TAALKESLRMSPGVASALPRIVPPSGAKISGQFIPGG (0) 1176790

1176862  TVVGMSSHFVHRSETVFEKPNEFIPDRWLGDKGSQ  1176966

1176967  LDKWLFSFSHGPRSCLGQN  (2) 1177023

1177088  LAWAELYFCFAHLYRKFDIEMDPSS (2)

         PRDLQWRDRFL  1177267

1177268  PEYIGPHLKAKITPVAS*  1177321

 

>CYP677B1 Mgr062 Mycosphaerella graminicola

47% to CYP677A1   Aspergillus nidulans

MAVSITLLLSGLVAAAVTQLAWTFLTNVFRHPLAQFPGPLLASTGWYKTWQEVFRGRNWIDVLRELHAQYGEVVRVGPNE

LQFADPEAYLDIYNASNRWSKEATLYQSFGEDASSFGFLHYREAKQRRDVLAPLFSRRAILDLQRVVRGIMDRLCVHLAK

ENAEGKSSDMLFALRCFTLDTIVTYCFAQDIHATEAKDFQAPVVVAMDASLPTFVVFKHFSAVRKLVFSFPGWLTKMTAP

GLGGLVDLQQLLGAQVKEVASNPQLLKQTSHPTIYHRLLDPEANEAAGVPSQQSLYEETQALLFGGADSVGNTVTIGLYH

VLQSPKMISRLKAELLSVWPDLQSQPRVEVFEKLPFLAAVIKESLRLAPGVTAPLPRVVPSSGATISGRHIPPGAIVGVA

QVMVHSNPDIFVSPEKFDPERWLGREASSLERWLVPFSRGPRACLGQTLAMCELQVAFAGLFRRFDIQLDEASTDSLAWR

ECFLPHFNASHTRAFCKPVVS

 

>CYP677B2P Mycosphaerella fijiensis

synt_with_mycgr.14__52

58% TO CYP677B1

frameshift with missing C-term

MAVTIAKAIGGLILAFICYKLGSTFYNAFLHPLTRFPGPKSVATEWYKTVEEVFKGRNWTDVLKELHGQDGDIVRVGSNE

LNFSNPNAFHEIYNNANRWDKEETLYHCFGEDRSSFWFLTYKEAKQRKDVLGDLQGLVKKNIERLCIALEKYGRAKKPSD

MLFALRCSTLDVVTRYCFAKDVKSTEVKDFQSPIVIAMEACLPSFVVFRHFEPIRQIVFSLPGWLTKLTNPALAGLVDLQ

ELLGAQVNEVVKNPQTLQKTPHATFYHRPMDQEANKATGVPSPGSLYEEAQALFFGGAESSGNTTMIGIY &

YHILQSPAILKRLQEELRSAGSDRSMIPPLEQLEKLPYLTAVIKE

 

>CYP677C1 Aspergillus terreus

48% to CYP677A2

ATEG_02825.1

MISASSTRGPFRAELEGGIPWRWSLLPFGAILTLIWVLLRRLHHAGLSRFPGPPLAAVSGLYKTYIEVLQQGCFVHKLEE

LHARYGDVVRTGPNELHFSSPAAYMDIYNMNNRWDKDPALYEAFGESDSSFGRLSYRDAKERKNVLQSLFSKKAAVQAHR

LVESKVVELCKAFEKSEGGPVDLLAAFRCLSIETIA (1)

YIFCGQSLGAIESPTFDAPIIQS

MDEGVSYIMLMKHFPILQLLMNHCPPWMSKLLSPKANGMVDFRN (0)

FLEKQVRSLVADRRELQKLPHTSTIY

HLLLGPEVANPPSQLSILEETQTQVFAGESSGLTLMHGSFHIIQTPRVYRKLREELMAAWPDLEQPPSLATLEQLPYLT (0)

NATVKESLRMGGGATSPLLRVVPQTGAKIDGKFVPPG (0)

TVVGSSSHFVHRNGDVFDRPHECIPERWLDERRADMEQYLVAFGRGPRRCIGEN

FAWVILLTAMAHFYRKFDVELDPSS (2)

PAKLEWKDRFVPVYSSPHLTVRLQKVSQ*

 

>CYP678A1 Aspergillus nidulans AN6485.1 poor match 53 clan

MNYVAYIPLLAIALLAWVIIRRLLFNPLLGIPGPWTARIFGFYEFYHNIW

RDGEWCKTFPELHKRYSSPVVRIGPNHVHINNIEAYEHIFRLGTDFYKDE

SFYTCADNHGSVFSLSDRDDHRERRKVLAPRFSKQAAELAAPKIQARLQD

LVQFMVKQSRKGEGCNITDLFRAVAINWVADVFLGDCGDWVNYEARKLDM

LEDIDGLSALIPTLRFFPYLATLNSLLPSSVVNYLTPSAVAGFKKICKDH

TRPLLNTPTKEMARRTEASVAELLIFHRLENTGKPPTLDYLAEEAFAFID

AGVDTTGGTLVTALYHVLKSPEILRKLRKELDEAMRRQAKSTDIDPRKLG

ELPYLNAIIKESHRIWPAIPGPLPRVVPPAGINIGSYFIPGGTTVSATHH

SLHYNETIFPEPHKFNPERWLKEEKSEGGRYLNPYSRGTRACIGINLAQI

QLRLTLTHLFGQYELELCDPVPSRHEWKDHFVAHPKEPIFVKFRERKGQV

ESVVQSGDEEGRGCKTNFVM*

 

>CYP678A2 Aspergillus clavatus

59% to CYP678A1 Aspergillus nidulans

ACLA_059710

MSDVARWLVDCCFSCSRGWLLLWSL

VVLVAHRLIQHLFFHPLRHIPGPWSATLCGWYEFYTNVWLDGQWCK

TYPYLHEKYR SPVVRVGPDHVHIKDIDTYEK IFRNGTNFYKDHTFYTCADNDGSIFSLCDRDAHRARRKALS

PRFSKQAAEADAPGILQQLRRMEAFIVRQSDEGKDCNINDLFRTFAINIVGKTLLGDCGDLVTYEDSKPELLDIVDGMSM

MIPTLRFFPYLAAINSVVPSFISNLLVPAGVLNFKKICEDYTRSRMDKPIRSDIERSSASVIELLVAHSHDITGGPPNLD

YLAAEAFTFIDAGVDTAGRTLAAAVYHILRDPIIEKKLRDELDKSDAWTSGALEVDVRKLWNLPYLNAIIKEAHRIWPAL

PGPLPRVVPPEGLQVDSYFIPGGTIVSATHHSLHFDEDIFPEPNRFNPERWLRNDRTDLDRYLNPYSRGSRACIGINLAQ

AELLLAVADMFRLLDLELCEPIPSGLTWKDHFVAEPISPILIRARPREVSF*

 

>CYP678A3 Neosartorya fischeri

58% to CYP678A2 Aspergillus clavatus

NFIA_045580

Note: this seq has no ortholog in A. fumigatus

MATMNFAPSSPLQLDAGVILQSLPIVAALIILFCILKLGHNFFRSPLKHIPGSRLSAFCGWDEFYSNIVLNGQWCKTYPE

LHRKYDSPIVRVGPNHVHVNDIDIYEKIFRMGTDFCKDHTFYTCANNDGSIFSITDRAKHRERRKVLSPRFSKQAAEAGA

PGVLRRLQQLVDYMTDQTRMGNTCDATDLFRALMINVVGEILFGDCGDLVEYGPSKPALLDSVDNLSSLIPWLRFFPYLG

TVASMFSTSISERLEPAGVYNFKQKCKEHTLPRMDQPISDAMDRSHASLIELLIAHSHQVQGKAPTLQYLTEETFTFIDA

GVDTSGRTIAAAYYIVRNPGVQQKLRAELDESSVTDQAGKHVDVKLLGTLPYLNAVIKEAHRMWPALPGPLPRIVPAEGL

TVGKHYVPAGSVISASHYMLHFNETIFPQPHEFRPERWLEGHSSEQDRYLNPYSRGSRACIGINLAQLQLQVTLGHLFSH

YQIQLCEPIPQSLDWHDHFVATPRSNVIIKILPRDQGY*

 

>CYP679A1 Aspergillus nidulans AACD01000170.1 Aspergillus nidulans FGSC A4 revised AT not AG

MTLTPLMESFHILAVLLSSQKCLMVHLSPLITAALATT

GLVLYLIATRLLFHPLAKVPGPRLAALTKWYEFYYDVVCDGEYVKHIEKLHIIY (1)

ASIIRTGPNQVHV

NDAGVYKRTFAVASPFPKSEFFYSSVGVGDAIGAMTDTKKHRVRRSILGP

RLRAKAIAPYTPSLQKLVIACMDIMAGKARQGKVINLLRYTRALTVDVIS

DFTFGRPMGVVNEAEEMPDLLNDLEVFGRHFHILKHFPISRKLLDIIPES

FARKLMPLPGFFELREKATTAVNEAVVERQAGKTISNNTDGASFLDLLLN

QIDENPSVLVDEGCAFITGGSDTTGFTMENATYLILSHPHILQSLLQELD

TSPHIRDTFDLDHILRLPFLTAIIKETLRLYTPAPSPLPRTVPAEGIDVH

GHFLPGGTILTHSLYLIHHNPVLFADPKSFKPERWLGTQGKVLEQYYVPF

SKGSRSCIGMALAYHEVYTYLSLLFSRFEMEIFETSQSDMDWREHFFVKR

NGQLRIRIVRDRWTGEAFPTRAVEADLR*

 

>CYP680A1 Aspergillus nidulans AACD01000006.1 Aspergillus nidulans FGSC A4 revised

MDLKESSLRFLASDIRSVIWRLATLISVVALLRTAWVVIYRIYFHPLAKV

PGPLLARITWLYSFWYNIVTKRFYLRVQELHEQYGPV IRITPDEIHLSDP

ENLEKIYFVGSRYPKDAAFYGAFGADRSVFTAPSPDVHRVKRAALNPFFS

RKKVLELEDIVQDKAEKLVGRMKKAFNSTGAIDLHHGFRAISVDVITDYA

FGDSYNFLDRDDFGIDFFNGFRDTGPALWTFQQFPVIQRVAKKMPFWLAE

RLSAPLALRRSQHMSSRAQVLKVKAAVERDEKVSRTTIFHELLRPDAAEG

YAVPNVDELSSEAQNILGAASDTTGNTLTIAAYNVVRNPDIYARLSAELK

EAFPNPEGRLEFVALEKLPYL (0)

TAVIKEGLR (2)

LSFGVPGRLPRVVPPPGAEFNGYHVPPG (0)

TVVSMSSWIIHHNENIYANADRFNPDRWLDPSRAKVLERYLFSFGKGTRGCIGMP (2)  140353

LAYCELYVTLGRMFREFDNLKTPAKSSEELIVDDGFSAYHPEKNNKFVFSLS*  140166

 

>CYP680A2 Nectria haematococca

fgenesh1_pg.scaffold_2000610

Necha1/scaffold_2:1945379-1947146

49% to CYP680A1 AN0338.1 Aspergillus nidulans

This gene model seems correct

MAVIFPNLGSLCHLVAQWWRTVVVGGAFYTIATIIYRRFFHPLAKVPGPFLPAVTKKYAWYFNVPCGGKF

YKEIERLHDVY (1)

GPIVRISPNEVHLTDPKHYDTIFGVKSDFYKDPGFYGALGIESATFTTISNDVHRRRRT

ALNPFFSRRKVLELESFDVIHDKVEKLCRMVSTDRAAGRPTNLHAAFRAISVDVITDYAFDDSWNQLDDE

DLGEWFSNMIRSSASMFWTFQAFASLRVPIQSLPDSVAKHMSPSVADFVACKD (0)

RTRKQVQDVQARINQGV

QPKRTTIWHQLLDPNATEGHVVPTINELVDEAFGICTAAADTTGNAMSMAAFHVVTNPTIYDTLKKELQE

AFPDPDARLSSSELEKLPYLTGVIKEGQR (2)

LSYGVLSRLPRVAPKGGVELEGYYLPEG (0)

TVTSMSAWMMHHNSEAFPNPEVFDPTRLTDPSTFHERDKCLVPFSRGRRMCIGHDLAWCELYVTLGTLFR

RFPDLRAYEVDAEDMVYVDYFTAYHPLEKRRFRVVSGDMEKLSIHA*

 

>CYP680A3 Aspergillus niger

e_gw1.1.1307.1|Aspni1

63% to CYP680A1

MISVHVLLLTVSVLSIVLLLRCVWTIAYRLFFHPLAHLPGPLLARATHLYAFWYNMQGGSFYLQVQKLHEQYGPVVRITP

DEIHLSDPENCEKIYYIGSRYGKDPQFYGAFGTHKATFTAPSPDVHRVKRSALNPFFSRKKVLELEDIVQDKADKLVRRM

RESFSSTGCIDLHHAFRAISVDVISDYAFGNCYGFLGKKNFGAEFFEMIRGFGPAFWFFQQFPAIQGLSLGTPFWLAKLT

SEPLTRMMLHREGSRRQILKVKDAVDRGEKGSRTTIFHQLLHPEAAEGHVVPTVEELEDEAYIMLAAAADTTGNALTIAA

YNVVRNAKIYKRLTEELKQAFHDSEAQIDFVALERLPYLTAVIKEALRLSCGVPGRLPRVVPDVGAEFNGYHVPPGVCNK

HGTIIVNFANIFLDCSSLSRSTCSLSARGLDNVSGCRICTDFCRLAYCELYVTLGRLFRQFDNLKTAQKSREELRYNDYF

SSYHPAEYNK

 

>CYP680A4 Aspergillus niger

fgenesh1_pg.C_scaffold_71000001|Aspni1

67% to CYP680A1 aa 316-512 seq gap upstream

PFWLAKLTSEPLTRMMLHRE (0)

GSRRQILKVKDAVDRGEKGSRTTIFHQLLHPEAAEGHVVPTVEELEDEAYIMLAAAADTTGNALTIAAYNVVRNAKIYKRLTEELKQAFHDSEAQIDFVALERLPYL (0)

TAVIKEALR (2)

LSCGVPGRLPRVVPDVGAEFNGYHVPPG

TVVSMSSWTMHHNEELFPKSETFDPNRWTDPVMSKSLEKYLFSFGKGSRQCIGMPLAYCELYVTLGRLFRQFDNLKTAQK

SREELRYNDYFSSYHPAEYNKFIFEKAS*

 

>CYP680A5 Aspergillus clavatus

ACLA_032120

63% to 680A1

AAKD03000006.1 895518-893676 (-) strand

MFLSSLPDIPHLAAIIGAVSVVRFIWVVIYRLYFHPLSKVPGPLLA

RAFFFYSFWYNYHDGRFYLQIEKLHQQY (1)

GPIVRITPDEIHLSDPENLDKVYYVRSRYGKSAPFYNGFGVNSSTFSTASHELHRVKRAALNPFFSRKMVFELEDVV

QQKAKKLVTRMQKAFVSSGRIDLHYAYRAISIDVITDYAFNNCFNSLDKEDFGAEFFTVIRDTVPALWFFQQFSFLHDFA

LNMPLWAARLCNQMLAKLMCYRADLKDQVLRVQASVSSGEKVERKTIFHQLLRPDAADAYTVPTTKQLADEAYVIVTAAA

DTTGNALTIATYNVVTNPKIYERLTAELKERFPDPEAEMDFAALEKLPYLTAVIKEGLRLSFGVVGRLPRVVPQPGAEFN

GYQVPEGTVVSMSSWIMHHNEGLYPNSETFDPERWLNPVTYNTLDKYLFAFGKGTRQCVGMQLAYSELYVTLGRVFRQFD

DLKTRKRTKEELIYDDYFSSYHPEAYSKFIFERASR*

 

>CYP680A6 Aspergillus terreus

67% to CYP680A5

ATEG_04863.1

MARADLLQLLGFTLVAATVVRGLWTVIYRLYFHPLAKVPGPFFARAFYFYSFWYNLNGGRLYLQIQKLHEQYGPVVRISP

NEIHLSDPENCDKIYYVGSRYGKDPAFYGAFGTEKATFTTPSPDMHRIKRSALNPFFSRKKVLDLEEIVQEKAAKLVGRM

RAAFESTGRIDLHHGFRAISVDVITDYAFNHCYGFLDEEEFGVEFFNMIRDFGPAFWFFQQFPAVQPLALGTPFWLAKLT

SGALTRMMLHHEGSRRQILRVKDEVDNGKKAARTTIFHQLLSPDAAEGYVVPTVEELKDEAYILVAAAADTTGNALTIAT

YNAVRNPKIYQRLTAELKEAFPDPAMKMDFVSLEKLPYLTGVIKEALRLSFGVVGRLPRVVPDPGAEFNGYHVPGGTVVS

MSSWIMHHNEDLFPQSEVFNPERWTDPEKARSLDRFLFSFGKGSRQCVGMPLAYCELYVTLGQVFRQFDDLTTPAKAREE

LLYNDYFSSYHPEQYNKFVFKRGSNGQ*

 

>CYP681A1 Aspergillus nidulans AN3609.1 34% to 551A1 53 clan

MSFLGLLLSAVFPVAIYGIFLVVYRLYFHPLRRFPGPKLAAATFWYEVYY

DWFKGPYPGSSWNLDRLHNQYGPILRKTPDELSIRDPDYVDVFFAGGRRD

RYSRQGKEAQGSVQSTLLGSDHRRRRGALTGFFSKRSLDTLEPFIMDKVE

QLSASVEENFLKTGNILEAGVAFGALTLDTITDYCFDQSFGCLSKPDLAP

EWRRTFWDMLESIPFLKNWTFFAEMFFWVPQWVVKHTNPAMEQFFIMQAA

IRAKVARVTMEWEQDQALQLQGKDPFMKGKRKRTIFYDILNSAVLLPEDK

TPKRMAEEAFGMVVAGGYTTGKAMANLMYHLHANPKWLERVREELDSLMP

SPDQPVKLSDLQALPYLTACIKENLRISNIITDSIMLVEPVDTLTYKDWV

IPPKTPIGMTLYHMHMDEQIYPEPKAFKPERWIKGAEAKHDLDKYFAPFS

KGTRGCLGVNLANAQMYLGLGVILRRFDFQLFDVVKERDVDTVRDCFVGL

ESPESKGVRLRVMDKRE*

 

>CYP682A1 Aspergillus nidulans AACD01000135.1 Aspergillus nidulans FGSC A4 revised 7/19/07

MAILWSYLFSSYAVIIGLVTSVYLLGLGIYRLFLHPLAKFPGPKYAALGR

WHEAYYDIYLSGKLIFWIEEQHKKYGPVVRIAPDELHILDADLWETIYTK

AGRVDKYDRMSSRFGNDTSVLATIPDSLHRVRRGALNPFFSRQRILGLQD

IIRQKLNIFIKRVEEYKALNAPMPINRGFLAFSEDVIMQYCFGHDYAALN

KRGWAPTLHDPIVNVTLAGNTALHFPIVPKIMNVLPQSWIAKLDPVYVPI

FQMQRIREIKDSLTGESPKIDKQTVFSDLIQGDLPASEKADRRLQDEAQL

VIGAGLATTGWALTVGTFYLLSNPKVLARLRRELDEAIPARNPENPSGAL

EWAELEKLPYLTGVIKEAVRLSHSTTSRNVRRLPKPITYKDWVIPPRTPV

SMTIPFLHLDEDIYPEPKSFIPERWLDNPKTKNGAPLERYFVGFGKGTRSCLGLN (2)

LAWCELYLVFAAFFRFFDFELYETDFSDIELQHDFFLPFPKWDSKGVRVFVKERSA*

 

>CYP682A2 Coccidioides immitis

64% to CYP682A1   Aspergillus nidulans

CIMG_09899.2

MSYVVDTLTYGGLIWAAYLAGLVIYRLYFHPLAKFPGPRYAALSRWHEIYYDVYLGGKFIFHIEDLHKKYGPIVRIAPDE

LHVIDSDFFDTIFTKAGRVDKYEWLSNRFGNATSTLTTAPDSLHKLRRGALNPFFSRKRIINLQELVREKIAILVGKINE

YKESGAPMTISRGYMALVEDIIMQYCFARDYNHLETPNWKPIFHDPLMAISMSGNAALHFPLVPKIMECLPQSWVVKLNP

LYVPIFRLQNDLSQQIRNLKEDKVDAKDGRATVFFELIHGDLPASEKEDRRVRDEAQLIIGAGLVTTSWALSVGTFFLLS

NPEALAKLRRELGEAIREDKYSKTLLQWAELEKLPYLTAVIKESIRLSYGTSSRNVRLSPNSLKYKDWVIPARTPVSMTI

PFLNHDEEIFPDSHAFNPERWLNSPKTSNGSNLDRYFVGFGKGTRSCLGSNLAWCELYLIFATIFRYFDFELYKTDKSDV

ELVHDYFLPFPKLDTKGIRVRVKQ

 

>CYP682B1 Aspergillus nidulans AACD01000150.1 FGSC A4 revised

MLSFGLANDAWGHPWVVLPLAVILYIV

VLGVYRLFFHPLSRFPGPVLAALTVWYEFYYDGIRRGLYTFEIQRMHE

KYGPVVRISPNELHVNEPSFIDELYAGSGKRRDKYPYSTCQFGIPDSVFG

TPGHDLHRLRRGALSRFFSKTSVTKLEPIIENAIGKLCTQLESYSGSQQP

VKMDMAFSCMTTDVVTEYAFAKSYNFLDSPTFEPNFHRPIVAGADLGPWV

KQFPVLLKVMNDLPKWILTRINPEAAVYIQFQEDLRRQIREVQSQVDKGE

SNGKIPTIFHELLTGDLPEQEKRIERLWQEGQIVVGAGTETTAWTLSVTL

FYLLDNPRIMRQLQEELERIIPDAAQSVTWHQLEQLPYLSAVICEGLRLS

YGVSSRLQRINPLGPLWVRSRDAKGGPHGKGRWVEYEIPKGTPVGMTSTL

IHTNPELFPDPHEFKPERWLDGAGKRHHSLDGYLLSFSRGSRQCIGINLA

YAELYMGLGLLIRRLGHRLELFETTSADVEIHYERFLPTPKDGTQGIRVL

VHPESE*

 

>CYP682B2  Aspergillus oryzae

          GenEMBL BAE64654.1

          56% to 682B1

MTLISLSLLALSLWIIIRVLVIIYRLAWHPLARFPGPKFAAATSAYEFYFDAIKGGQYTFEIGHMHKKYG

PIVRISPHELHINDPGFIEELYPGPGKPRDKYAYATGQFG (2)

IPD (0)

SVFGAVSHDLHRMRR

GALSPFFSKAAVTKLEPVIYSAVDKLISRIEEVVESTGFVDLTMAFSCMTTDIVTQYAFAESSRFLENPD

FTPNFHEAILAGTRMGSWARHFPILFPVLRSIPIDILSRMSPETGVFLRWQESMRKKVSEIWQDQSALPV

KDKNVSPFGSTIFHELFHSDMPDSEKHPGRMWQEGQIVIGAGTETTAWTLTATTFFILDNPNILSKLRKE

LAATMPNRYEKPSCRELEALPYLIIIQEGLRLSFGVATRLQRINSEAPMIFRQKKTNDTIEEKVWEIPTG

TPVGMTAALVHLNPELFPDPHEFRPERWLDQDGQLHRGLDKYILSFSRGSRQCIGINLAYSELYMGIGIL

IRRLGDRMQLFETDRTDVDMVEDCFVPVPRRESNGVRVRLSAQ

 

>CYP682B2  Aspergillus flavus

          AAIH02000253.1 99% to CYP682B2 Aspergillus oryzae

          AFL2G_04898

12469  MTLISLSLLALSLWIIIRVLVIIYRL

12547  AWHPLARFPGPKFAAATSAYEFYFDAIKGGQYTFEIGHMHKKY  12419

12363  GPIVRISPHELHINDPGFIEELYPGPGKPRDKYAYATGQFG

       IPD

       SVFGAVSHDLHRMRRGALSPFFSK  12019

12018  AAVTKLEPVIYSAVDKLISRIEEVVESTGFVDLTMAFSCMTTDIVTQYAFAESSRFLENP  11839

11838  DFTPNFHEAILAGTRMGSWARHFPILFPVLRSIPI  11734

11657  DILSRMSPETGVFLRWQE

11533  SMKKKVSEIWQDQSALPVKDKNVSPFGSTIFHELFHSDIPDSEKHPGRMWQEGQIVIGAGTETTAW (1) 11360

       TLTATTFFILDNPNILSKLRKELAATMPNRYEKPSCRELEALPYLI (1)  11136

       IIQEGLRLSFGVATRLQRINSKAPMIFRQKKTNDTIEEKVWEIPTGTPV  10925

10924  GMTAVLVHLNPELFPDPHEFRPERWFDQDGQLHRGLDKYILSFSRGSRQCIGI  10766

10707  NLAYSELYMGIGILIRRLGDRMQLFETDRTDVDMVEDCFVPVPRRESNGVRVRLSA*  10537

 

>CYP682B3 Uncinocarpus reesii

Remove intron in I-helix GTSCPTQQSYMSLNHIA

53% to CYP682B1, mid region has some questions remaining

UREG_02363.1

MALLTDLGQFVAQHPYVVLCLLLVSFFSGRSIYRLYFHPLAKFPGPKLAAVTLWYEYYYDGIKGGQYTFEILRMHEKY (1)

GPIIRISPHELHVNDPAFIDTLYAGSGKRRDKYPYYALQFG (2)

LPG (0)

SLFGTSDHDHHRLRRSALRRFFSKASITQLEPLIAKKIRDMCAIIETHAGTGKPVAISEAFSCLSSDIA

TEYAFGRSTNFISPDNPKFEKNFRRTIETGSMVATHSKQFPWIRPLSKMIPMY (0)

GVGE

QVKALMDRTASGKEEG

GNTIFHELLSGQLPPQEKTFKRLEEEGQLIVGAGTETVGW

ALSVTIFYLLSQPETFAKLRGELEEAIPD

PSILPPVTVLENLPYLI (1)

IIIAEGLRLSYGVVSRSQRISPHEPLIFKPGPNYQKGCTEYVIPPGQPVGMSCYIT

HHDPELFPDSRNFKPERWLDNEGKRHRHLDPYLMSFSKGSRQCIGINLAYAELYLVMSAVIRIFGDRFKLFETTLKEVEA

KSDYFLPIPESEKGIRVLVRAK

 

>CYP682B4 Coccidioides immitis

70% TO CYP682B3

CIMG_08088.2

MALLAEIVQFIPQHPFAVLCLLVVFALSGLSVYRLFLHPLAKFPGPKLAALTLWYEYYHDGIRGGQYTFEIQRMHERYGP

IVRISPHELHINDPAFIDTLYAGGGKRRDKYVFYASQFGLPKSLFGTADHYHHRLRRSALRRFFSKASVTQLEPLITKKI

DTLCSLIQATAGSGRPVSLSDAYSAITSDVATEYAFGTCTNFVCPSNPYFERNFRVTLVKGLMVATHSKQFPWIRTISKM

IPMC (0)

EISKQVKDVMDGVSNGNEKGHNTIFHELLTGSLPPQEKTFTRLKQEGQLIVGAGTETVG

WSLSVITFYILAHPAVLEKLRKELEEAIPDPSILPSVTTLENLPYLSAVVAEGLRLSYGVISRSQRISPYEPLIFEPASD

QENHIKHVIPAGTPVGMTSYLIHHNPEIFPDSSKFIPERWLDQDGKRHRHLDPYLMTFSKGSRQCIGINLAYAELYLIIS

SLIRRFGGRIELFETTVRDVVPKKDYFLPVPDGDAVVQVLVRED

 

>CYP682C1 Aspergillus nidulans AN6787.1 34% to 579A1 35% to 58A1 53 clan

revised 7/19/07

MELLRLLLDHPYAVAGAAVSVYIASIVIYRLYLSPIAHFPGPRLAALTVM

YEFYWEAIRHGKFTFHIGELHKKY

GPIVRISPTELHVNDPEYYEVIYSRDSPRNKYPYYQRTFNAPYAL

ITAEDHYRHRLLRSQLNPFFSIQRIRQLEPTLKALVDKLCRRLEELKGTG

QPIDIEYPLTCYTTDVITDYTMGEGGYHYLDEPDFIPQWQHMLCGTAKTL

VFIRPIAFLLPVLVAMPEALTAWLNPGMELFFAFQHRCRKRIAEITKRHR

ENGPLETKDGRQNLFDNVLNSNLPEQEKSEARLAQDMQVFVSAGAETTAK

AMSYIMFYLHNEPALLQRLKDELAPLGNDPSLVQLEQLPYLTSVMLEGLR

LSYGVTARLPRIAPYNALKYKDWTIPPGTPISMSCLLMHHDESIFPDSYR

FNPDRWMDPTERKHLEKYMVAFSRGSRMCIGMHLAR

SEILLVISSLLRRLNFELYETTVEDVRVAHDIFIPFVKLDSKGVRFLIK*

 

>CYP682C2  Aspergillus fumigatus Af293

          GenEMBL XP_748206.1 also EAL86168.1

          69% to 682C1

MELFRLLLSHPVSSAVALGAVYLLWVVLDRLYLSPIAGFPGPKLAALTHLYEFYWDTICCGQFTFQIGRL

HEKYGPIVRISPTELHINDPDYYEVLYSRDSPRNKYEYYQKTLNAPLALLNTIDHHLHRQLRAQLNPFFS

STRIRRQEPAIKALVNKLCRRLDELKNTGQPLNIEHALTCYTTDVITDYSMGDGYHYLDAPDFIPQWHGT

LNGTAKTMVFIRPVAWALPLLLALPEAVTAWLNPGMELFFDFQRRCRKMIKRIVAAHNEKGNQGVQNEGF

VNIFDDILRSNLPAKNKSETRLAQEMQVLVSAGAETTAKAITYILFYLLNDPETMAKLKAELETVGEDPA

LVQLEQLPYLTGVMLEGIRLSYGVSARLPRIAPYNALKFRDWTIPPGVPVSMSCLLMHHNETVFPDSHRF

KPERWLDPAERKRLEKYMVAFSKGSRQCIGMHLAKAEILLAVSTLLRRMDLELYETTVEDVAIKHDIFIP

FPRMDSKGVRVLIKG

 

>CYP682C2 Neosartorya fischeri

97% to CYP682C2  Aspergillus fumigatus = ortholog

NFIA_040740

MELLRLLLSHPVSSAVALGAVYLLWVVLDRLYLSPIARFPGPKLAALTHLYEFYWDTICCGQFTFQIGRLHEKYGPIVRI

SPTELHINDPDYYEVLYSRDSPRNKYEYYQKTLNAPLALLNTIDHQLHRQLRAQLNPFFSSARIRRQEPAIKALVNKLCR

RLEELKNTGQPLNIEHALTCYTTDVITDYSMGDGYHYLDAPDFIPQWHGTLNGTAKTMVFIRPVAWALPLLLSLPEGVTA

WLNPGMELFFDFQRRCRKMIKRIVAAHYEKGDQGVQNGGFVNIFDDILRSDLPAKNKSETRLAQEMQVLVSAGAETTAKA

ITYILFYLLNDPETMAKLKAELETVGEDPALIQLEQLPYLTGVMLEGIRLSYGVSARLPRIAPYNALKFRDWTIPPGTPV

SMSCLLMHHNETVFPDSHRFKPERWLDPAERKRLEKYMVAFSKGSRQCIGMHLAKAEILLSVSTLLRRMDLELYETTVED

VAIKHEIFIPFPRMDSKGVRVLIKG*

 

>CYP682C3 Aspergillus clavatus

82% to CYP682C2

ACLA_003860

MELIRLLRSHPVAALALLGSGYLLGVILYRLYLSPIARFPGPKLAALTHLYEFYWDTICCGQFTFQIGRLHEKYGPIIRI

SPTELHVNDPTYYEVIYSRDSPRDKYGYYQRTLNAPLAILNAIDHYRHRQLRAHLNPFFSTLRIRKQEVAIKALVDKLCR

RLGEFQNSDTPVNIEYPLTCYTTDVITDYTMGDGYHYLDEPDFIPQWHGTLNGTAKTMVFIRPVAWALPFLLALPESVTA

WLNPGMELFFDFQRRCRRMISAIATAHRESGGKMAQDGGRLTIFDDILNSDLPARDKSEARLAQEMQVLVSAGAETTAKA

ITYILFYLLNDPPTMQKLKQELATLGPDPSLVQLEQLSYLTGVMLEGIRLSYGVTARLPRIAPYNALQFGDWTIPPGTPV

SMSCLLMHHDESIFPQSHLFKPERWTDPAERKRLEKYMVAFSKGSRQCIGIHLAKAEILLAVSTLLRRMDLELYETTVED

VQVKHDIFIPFARMDSKGVRVLIKG*

 

>CYP682C4 Aspergillus terreus

78% to CYP682C1 Aspergillus nidulans

ATEG_04990.1

MELLRLLLSHPYVTAAAVTVGYLLSAAVYRLWLCPIAHFPGPRLAALTVMYEFYWDTICCGQFTFHIGELHKKYGPIVRI

SPTELHVSDPDYYEVIYSRDSPRNKYNYYQRTFNAPLALITAEDHFRHRLIRSHMNPFFSTMRIRQQEPVLRALINKLCG

RLEEWKNTGKPLHIEHALTCYTTDVITDYTMGEGYHYLDEPDFIPQWYHTLTGTARTLVFIRPVAFLLPLLLAMPESVTA

WLNPGMELFFDFQRRCRKLIHKIATDHLEKGSHAVDQKGRLTLFDDVLSSDLPEQEKSEARLAQEMQILVSAGAETTAKA

MTYILFYLLDNPELLKKLKAELEPLGPDPALIQLEQLPYLTSVMLEGIRLSYGVTARLPRIAPYNALKYKDWTIPPGTPI

SMSCLLMHHDESVFPDSYRFDPERWTDLTERKRLEKYMVAFSKGSRQCIGMH (2)

LARAEILMVISTLLQRFEFELYETTVEDVRVKHDIFIPFTKLDSEGVRVLIKG

 

>CYP682D1 Aspergillus nidulans revised

MLEKGGFLRLFEGAFPASSKSQKLQKPSKSRMLHLVPYIFLAWVAYVVSL

VIYRLWLSPLAKFPGPKLAAATLWYETYYDAFKWGKYTFEIAKMHEKY

GPIVRISPHELHINDPDYYEVLYSRDSPRNKYEYFTK

QFPVAGSSIATVDHYHHRVIRSNMNPYFSITRARKQEPLVQALVNKLIER

LQAYKGTQTPINLQHAFTCFATDVVSDYSMGVGFHYLDEPDFIPDWSDTL

AWSVKNGVYLKAFPWSGALLNSLPPWLLSRIYPGMGLVFQFQDRCNRIVQ

RIMEEQKERGYEEVKNQFDHPTFFHDVLTSNLPKELKTPDRLAREVQAVI

GAGSETTGKMLTWTMYYLLENPDKMNALKEELNRLDPDQTATLVDFEKMP

YLTSVMLEGLRLSYGSSSRLQRIAPDRDLKFKEWSIPAGTPVGMTSVLIH

HNEEIFPDSHEFIPERWLDPEKRKYLEKYMVSFTKGSRQCVGMNLAKSEI

LLCLPHVVRKVNMELYETTRDDVTLAHDLFLGFAEEGSKGVRVLIR*

 

>CYP682D2  Aspergillus oryzae

          GenEMBL BAE55187.1

          68% to 682D1

MVHPSVLVEALVMFRPIAQFLLFPPFCMFQFLFQPWIIALAGAGYLISLAVYRLWLSPLAKFPGPKLAAL

TLWYELYYDIYCEGQYTFQIIRMHEKYGPIVRISPWELHISDPDYYEVLYSRDSPRNKYEYYTRQFGLTK

TAMATVDHYHHRLLRSNMNPYFAMTRIRKLEPLIQGLVDKLCDRLREFKGTGTPVALQYPFTCFATDVVT

DYTMGAGFHYLDEPDFVPRWSRTLSGVAKSGVYIKPFPWLIKVFNALPESWLSWLNPEMDLTFHFQRRCR

EVIASIMEEQNANGYDKVKSQFSHPTFFHDVLNSNLPPEEKSPERLWQEVQVVVGAGAETTGKALTWTMF

YLLHSPDKLQKLREELNQLDPDRTATLLDFEKMPYLTSVILEGLRLSYGLSTRLQRVAPDRALQFREWSI

PAGTPVGMSSTLMHHDERIFPDSHKFIPERWLDLEQRKHLEKYMVAFTKGSRQCIGMNLARSEILLALPK

VLRELDFELYETTLEDVTLAHDMFLPFPKMDSKGVRVLIK

 

>CYP682D2  Aspergillus flavus

99% to CYP682D2  Aspergillus oryzae

AFL2G_00090

MFQFLFQPWIIALAGAGYLISLAVYRLWLSPLAKFPGPKLAALTLWYELYYDIYCEGQYTFQIIRMHEKYGPIVRISPWE

LHISDPDYYEVLYSRDSPRNKYEYYTRQFGLTKTAMATVDHYHHRLLRSNMNPYFAMTRIRKLEPLIQGLVDKLYDRLRE

FKGTGTPVALQYPFTCFATDVVTDYTMGAGFHYLDEPDFVPRWSRTLSGVAKSGVYIKPFPWLIKVFNALPESWLSWLNP

EMDLTFHFQRRCREVIASIMEEQNANGYDKVKSQFSHPTFFHDVLNSNLPPEEKSPERLWQEVQVVVGAGAETTGKALTW

TMFYLLHSPDKLQKLREELNQLDPDRTATLLDFEKMPYLTSVILEGLRLSYGLSTRLQRVAPDRALQFREWSIPAGTPVG

MSSTLMHHDERIFPDSHKFIPERWLDLEQRKHLEKYMVAFTKGSRQCIGMNLARSEILLALPKVLRELDFELYETTLEDV

TLAHDMFLPFPKMDSKGVRVLIK*

 

>CYP682D3 Uncinocarpus reesii

67% to CYP682D2    Aspergillus oryzae

UREG_00188.1

MDLRQFVSGQPVLAAAVAWLLYVISLVVYRLWFSPLSKFPGPKLAAATLWYEFYYDALCHGRYTFEIMKMHQKYGPIVRI

SPHELHIDDPEYYDVLFSRDSPRDKYEYYTRQFGTPMAAISTVEHAHHRLRRSNMNPYFSMGRIRKLEPTIQALVDKLCA

RFREFKGTGTPLVVQHVYTCFSTEVISGYTMGSGFNYLDEPDFVPHWCDTLSGIAKATVFFKQYPLLLHIMKALPENFVG

KMDPGMNLMFIFQRRCKVLIDSVIAEHAAPDYAEKRKSYDHPTFFHDILESNLPPEEKGPERLSQEIQGVIGAGSETVAK

TLSWLTYYLVANQDKLDKLLEELNRLDPDQTASFVDFEKMPYLTSVMLEGLRLSYGVSSRLQRVAPDRNLQYKEWSIPAG

TPVGMTSVMMHHNEDIFPDSHSFIPERWLDPAQRRYLEKYLVAFSKGSRQCVGINLARAELLLAIPKLFRQLKFELYETT

VEDVTLAHDLFLPFPKKGSKGVRVLVV

 

>CYP682D4 Coccidioides immitis

81% to CYP682D3   Uncinocarpus reesii

CIMG_00083.2

MSSFFILRLTVKSFWSVWWSREMEALPLIKEHPFLTATAAYLVYVTAVVVYRLWLSPLSKFPGPKLAAATRWYEFYYDAI

CHGKYTFEIIKMHEKYGPIVRISPHELHINDPEYYDVLYSRDSPRDKYSYYTGQFGTPMAAISTISHAHHRLRRSNMNPY

FSMARIRKLEPAIQILVDKLCNRLKEFKGTGVPIVVQYPYTCFSTDVISDYTMGTGFHYLDEPDFIPHWSETLSAIAKAT

VFFKPYPWLLYWLKALPEGLVGRVDPGMNLFFVFQRRCKVLINSVIREHSEPDYEEKRKSYDHPTFFHDILDSNLPPEEK

GPERLAQEIQGVIGAGSETVAKTLTWLTYYLLENKDKMDKLLEELNRLDPDRTATVVDLEKMPYLTSVMLEALRISYGVS

SRLQRICPDRDLQYKEWSIPAGTPVGMTSVMMHHNEDIFPDSYNFVPERWLDPDRRKYLERYLVAFSKGSRQCVGINLAR

AELLLAISKIFRELKFELFGTTREDVTLKHDLFLPFPKVGSKGVRVLVVD

 

>CYP682D5P Aspergillus oryzae

50% to CYP682D4 N-term part, 100% to CYP682D5P Aspergillus flavus

VAGAFYVLWLALQRLWLSPIAHFPGPKLAALTMWYEFYYGSFLEGQYTFRIAEMHRKYGPIVRISPY

ELHIDDAEYYETLYSRDAPRDKSLHLTGMFGAPASAFGTVDHRRHRIRRQPMNPFFSQQRIRQLEPM

LRDMVDKLCDGLRAWKDRHTPLHMYHPFNAFTTDVVVEYTMGHSSHYLDDSDFSPQRSKTMQAIVNA

GIQFRQFRWFISLFELLPR

 

>CYP682D5P Aspergillus flavus

50% to CYP682D4 N-term part

AFL2G_01379

VAGAFYVLWLALQRLWLSPIAHFPGPKLAALTMWYEFYYGSFLEGQYTFRIAEMHRKYGPIVRISPY

ELHIDDAEYYETLYSRDAPRDKSLHLTGMFGAPASAFGTVDHRRHRIRRQPMNPFFSQQRIRQLEPM

LRDMVDKLCDGLRAWKDRHTPLHMYHPFNAFTTDVVVEYTMGHSSHYLDDSDFSPQRSKTMQAIVNA

GIQFRQFRWFISLFELLPR

 

>CYP682D6 Aspergillus terreus

63% to 682D2

ATEG_08919.1 revised

MKEILLLYAQAAGLVGAAYFLVLAIYRLWLSPIAHFPGPKLAALTLWYEFYYDTILHGQFTFEIARMHRRY (1)

GPIVRISPYELHIDDPEYYEVLYSRDSPRNKYEYYVRQFGQPKAAFSAVEHSRHRLLRASMNPFFSLTRIRRHESRIKALADK

LTQRLNEFQNTGRPMVIQHAYTCFTTDIVSEYVAGQDFHYLDSPDFMPQWCETLSGIAKAGVFFKPFPWLHSVMKCLPQS

WVSRVDAGMGLFFSFQQRCASLIQSITDSENNQPGKSANNTRAHTAFFHEVLKSDLPPSEKSAERLAQEMLIVVAAGAET

TAKALTWITFHLLNKPELLQRLLDELQRLDPNQTASLLQLEQMPYL (0)

NGVILEGLR (2)

LSYGISSRLPRIAPDRALHYKEWTIPPG (0)

TPVGMTSVFMHHNESIFPDSHRFMPERWLDLQTRKHL

EKYMVAFSKGSRQCIGMNLAKSEMLLAVSKVFREVKFELFETTVEDVTMA

HELFLPFPKVGSKGVRVIVRS*

 

>CYP682E1 Aspergillus nidulans AACD01000079.1 Aspergillus nidulans FGSC A4 revised 7/18/07

MLSLDLVFSFPAWALLLVLTLLYTLYLA

TTRLLLSPIR

HIPGPTLAALSFWPEFYYDVVQRGQYFRQIDKMHQTYGPLVRINPFEIHI

QDPSFYPVLYTGPTRRRHKWLWAARMFGNNTSAFATVRHEHHRLRRSALN

PLFSKSAIQRLTPHLQHTLARLCSRLDGFAFTRQDVDLGIGLTAFAADVI

TEYCFGQSLELIGKDNFGKEWIDMVSAPSELGHLVKQCPWILVVCKWAPKALVRALLPGVALLFQIQE (0)

RMSAQIQPLVDRAAAVDKPA

DPLTVFDFLLSSTLPQHEKTVDRLKGEGQTLIGAGTLTTGNALKTIIFHVLNDPDI

FRKLRAEVDGALENMDILSMSDTAYLERLPYLSACIKEGLRISYGVTHRL

QLIAEEPLIYSGVTIPAGTPVGMTSIFMHDNPVVFPQPREFRPERWFEAD

FETVQAMNRHFVPFSKGSRMCLGMNLAYAEIYLVLAVLFRRYEISLSGVT

REDIEMAHDFFDPAPKEGARGLIVQLQKRG*

 

>CYP682F1  Aspergillus niger

          XM_001397274

          43% to 682C2, 42% to 682D2, 41% to 682D1

          35% to cytochrome P450 Myrothecium roridum tri4 gene CYP58A2

          60% to DQ054596 mid region fragment of Aspergillus ochraceus in

          ochratoxin synthesis

MSIDTLLYTSLAVGAVYLASVALYELYWSPLAHIPGPKLAACTR

LYEFFYDVVCGGQYTFKIADLHKQYGPIIRISPREIHIHDPNYYETLYATNSPRNKDP

WFTAHFGVDESAFSTLDYRLHRSRRAMIAPFFAKARMDGAQPLIKANLAKLIRHLDAH

ASSQSVLKVEVAYNSFTGDVITGYTSYRSFEYLETTDMVPIWSETVRNLVESGMLSRH

LPGFFPLLAWAGSRCIAAVYPKLLPVIAFRMKCAQEVNFMWTNSEEGKKEAIQSGCSE

PALFPELVSRASSAPDITEERLLHEFITIVAAGTETTAHTMTVCTFHIAHNQSILDKL

REELDNRFHSNADMDLQTLEQLPYLTGIIYEGLRLSYGLSHRLQRISPIDPLKYKDIA

IPPNTPVGMSSALMHHDESIFPQSHEFIPERWTDPNDRRRLNKYMVSFSKGSRQCIGM

NLAFAELYLGIATLFRRYDMKLHDTTLDDVQLHGDKMLPRAKNGSKGVRVTLRRAQSVG

 

>CYP682F2  Aspergillus ochraceus

          DQ054596

          P450 in the ochratoxin biosynthesis pathway

          60% to CYP682F1 (mid region)

EVQRIQSLIQSKLQKLNTRLSEYAHSGEPLKVDVAFNCFTADII

TSYTSFRAFNYLDDPEMVPIWSETIKNLVEIGMIARHLPGFFPLLASMGMKWVKRVYP

KLLPVIAFRMKCAQEVNFMWENEEEAKLDFEKNRLSQEPALFQEMVAKAPDT

 

>CYP682G1 Aspergillus niger

e_gw1.2.437.1|Aspni1

44% to CYP682B1

MTLFGELSYLSLASITLTVFLAYVIGCIVYRLYLSPIAAFPGPPLAAVTWYYEFYYDAICGGRYIFKIKDMHKRYGPIVR

INPEELHIETSTFYDELYAPGGKKRRNLNPQFEHQFGAPNSTFATVDHDLHRMRRSALSPFFSKASVRRLQPVIWEKVDK

LLRRLDEFKNTGQPVRMDIAYAAFTNDVDVIMEYSFNHSRDRLQKPDFGEPAQEAVLAMSKVVHWMKHYHWLIYLAQLIP

PWIVSWISPTLQSFTRENVAQVHQIMNDKDRLGVGKQSKTIFHDLLANPDLPVSEKSVEQLGQEAKILVAGGTETTSWTL

SVITYYLLSQPHTLSRLRAELIASIPDPTASVGIEVLEHLPYLTAVIQEGLRLSYGSVGRLARVSPDEAVTFHDEKRAKD

WIIPPGTPMSMNSVFVHHDPTIFPEPSKFSPERWIEAEKEGKRLNRYLVSFTKGSRSCVGINLAYAELYICVSRIFRLYS

CTEDEMSGKGGWLELFETSNEDVEFATDMFIPATRQGSKGVRMLVKGI*

 

>CYP682H1 Mgr071 Mycosphaerella graminicola

44% to CYP682B1 N-term from JGI model e_gw1.2.1462.1|Mycgr3

MPNLELWTALSYFT

MAEIVVSGLLLYFFTLAVYRLYFSPLAKIPGPKLAALTLWYEFYYDVVLKGQYTWKIGEMHEKYGPIVRINPFEIHINDP

DYYNEIYAGPTRRREKWAWSAAMFGNSSSHFGTIPHELHRRRRAPLNPFFSRASVTRLEPMLRQVVEDLSTRLEQFRESQ

KPVNLRHAFAAVTMDVVTDYAFGTSYKCLEQPDFAPMWAEAIDSVSEQSHMNKQFPIVLQMMRMMPLWVVERTAPDVMRL

IKFQMDMATQVSLQMSGKSADNKVSDHPTIFHELLQPGALPESEQTLPHLVNEAQSIVAAGQVTTTHYLNTTAYHILARE

DVLKRLKAELKEVMSDGSLPSWQALEQLPYLSAVVLEGYRMSHGPVHRLQRVAPDEDLVYKDYVLPPGTPVGMTSIFIHE

NPSLFPDPRAFKPERWLEPGAKERLSNYLVNFSKGTRGCLGMYLAQAEIYLTLAAVFGRFDMEIFETTRADIDVAHDFFN

PQPRKGSVGLRVLVK

 

>CYP682J1 Uncinocarpus reesii

40% CYP682B1   Aspergillus nidulans

top hits are all CYP682s and CYP58s

UREG_01137.1 missing the C-term, added back

MQFSSGMLVPFGLLLCGYVVWLVLDRLFWSPLAKFPGPRLAALTNWYEFYYDVILQGKFTAHIQDLHKQYGHIVRITPTE

LHVDDPEFYEQLYSRNGRRDKYAYFSGRFGYASDTFSTVHHDLHRLRRKPLGPMFSTQKISEFQPVIRAKIEKLCQKLTE

YHDGKIVTLNRAWMALTTDIITEYAFAKSYDQLDSPNFRDTLHESLVAIYVTGHFALHFPIVFPVLDALPEWLVLKMQPD

LVSVVGMRKDLARRVNDIRNGVNDSHKTVKHRTIFHEVLNSDLPEGQKTDARLGDEAQLIVAAGLITTSWALTVASFHII

SDRAILTKLRREVNDAQSGSDDPLDWHVLEQLPYLNGCVREAIRLAHGITTRNPRLAPDSELKCGDWVIPRNTPVSMTNV

DTLMNPTIFPNPEKFIPERWIKDPSLDRYFVAFGKGSRQCMGIN (2)

LAMAELYSAIATVFTRFTFELYDTTHSDIVMKHAYLVPYANWDSKGVRALVHPADHAQR*

 

>CYP682J2 Coccidioides immitis

85% to CYP682J1   Uncinocarpus reesii

CIMG_01197.2 revised

METPSWAFLSLGFTLLTAYIGWLVLSR

LFWSPLARFPGPRLAALTNWYEFYYDVILQGKFTEHIQDLHKQYGRIVRITP

SELHVDDPEFYEQLYSRNGRRDKYAYFSGRFGYASDCFSTIHHDLHRLRRKPLGPMFSTQKISEFQPVIRAKVDKLCQKL

SEYSDGKVITLNRAWMALTTDIITEYAFAKSYDQLDSPGFRETLHEALVAIYVTGHFALHFPVVFPILDALPEWLVLKMQ

PDLVSVVGMRKDLARRVNNIRNGVNDSYKTVKHRTIFHEVLNSDLPDDQKSDARLGDEAQLIVAAGLITTSWALSVASFH

IITDSSVSKRLRDEVNLAQSRSEGPLDWRTLEQLPYLNGCIREAIRLAHGITTRNPRLVPDSELVYGEWVIPRNTPVSMT

NVDTLMNPEIFPDPEKFVPERWIEDPTLDRYFVAFGKGSRQCMGIN (2)

LAMAELYTAIAIVFSRFTFELHETSHSDIVMKHAYLVPYPRWDSKGVRAIVHSVNQP*

 

>CYP682K1 Uncinocarpus reesii

44% to 682D2, 46% to 682D1

UREG_06063.1

MVKVLTMACILAIAYVVGVVVYRLYFSPLARFPGPKLAAATLWYEFYYDVILKGQYTFKIKDLHQKYGPIIRISPY

ELHINDPEYYSTLYSHSSPKDKYYYYVKPFDFPRSSFGTESAQVHRFRRGAMNPFFSRGKVLQHEGLVQELVRKFCDRIE

GFGKTSQIVPLSLGFTCLTTDLITSFVLKRSYRFLEATDWNPHWGQTLRDASELGMMSRQVTWMLPILKSLPRAWAEALN

PGLVLFFSLVQRTYQRIAEVEQQRDMAEKGNACSERGYTLIDQILDSKLPDDEKTPERLAEEIRSAIGAGTETTSNALTV

TVYHILRDPSKTQRLRQELEGIEPNPDREIKLRELEQLPYL (0)

SSCILEGLR (2)

LSYGVSTRLQRKMDNQVITYKEYVIPPG (0)

AAIGMTSILQHHDESIFPDSYSYVPERWLGEEKRKRLERYLVSFSKGSRRCIGMS (2)

LAMAQLYITIASMFRRFNFELYGTTRADIEVRHDLFIPRPKNLKT

LGLRATAERREPVMTRH*

 

>CYP682K2 Coccidioides immitis

68% to CYP682K1

CIMG_09894.2 revised

MAYTFTVARMSIIGYIVGTLVYRLYFSPLSKIPGSKLAAATLWYEFYYDVILGGQYTFKIKELHQKH (1)

GPIIRISPYEVHIDDPEYYHTLYSHRCPRDKCDYHVKPFNLPKSSFGTESARVHRLRR

GAMNPFFSRGFVLKHESLVQDLVQKFCRRVEEFARDEEIVPLSLGFTCLT ()

DVITSFVVNRSYRYLEASDW

HPHWGQTLKDASKLSVITKQVAWTLPIPKCFPQSWAKALNPGLALFFDLTQRTHRRILEVLKEREQERGVSGPGRRKTLI

DQLLDSKLPQEEKSMERLAAEIRSAIGAGTETTSNSMTYHLLANPQKLQRLRDELLKLGPGREANLCELEQLPYL (0)

SSCILEGLR

LSYGISTRLQRKSDHTEIVYKAYRISPG

TAVGM

TSVLQHHDEAIFPDSYGYVPERWLNTEERKRLEKYLVTFSKGPQRCIGMS (2)

LALAELYLTVTNIFRKFNLELHNTGRNDIDIRYDLFIPRPKNLKTAGVQVRVKGLDN*

 

>CYP682K3 Histoplasma capsulatum G217B

60% to CYP682K1   Uncinocarpus reesii

HCB05195.1

MLNEAGHLTFTQGVLVSICSFFIIYLTGGVIYRLYLSPLAKFPGPKLAAATLWYEFYYDAVRRGRYTFKIKELHQKYGPI

VRISPYELHIDDPDYYRVLYSYSSPRDKYPYYLKPFGFPLSAFGTENHYQHRRRRGALNPLFCNRKVAQREGLVHQLVHR

LCEYLEQFRVTGKTVSLSLGYTCLATDLIVSYVLDENQQWLDAPEWLPHWRWALRTLSEMVMVSRQVTWLLEILKHFPRA

WVASSNPGLGLFFELEERFRRKIDEIQIDRGRISQRAETEISKGYTLIDQILDSNLEVEEKTPERILQEIRSTTTAGIET

TSNALTVITYHLLDNPDKLQKLQNELSILEDGGNSEQQVHELGKLPYLTSVILEGLRLSYGVSTRLQRKSPEQAIQYKNY

CIPPGTPVGMTSVLMHHNEAIFPDSCKFVPERWLDLQERKRLERYLVSFGKGSRRCVGQRLAETTLLIAIATIFRKFELQ

LWGTLKNDIEISHDLFVPRPKNTKSIGLQVRVLKKY

 

>CYP682L1P Coccidioides immitis

pseudogene

44% to CYP682A1   Aspergillus nidulans

45% to CYP5095A2 A. niger

CIMG_08072.2

MALQHWYHSCSETHREGVIAAALAIW

ILYLVSLAVYRLYFSPLAKFPGPKLAALTRWYEAYYEIVKIGQYSFKVDELHDKF

GPIVRVTPDEVHIRDAQFYDQM

YAKNPRVSKPG*NKIFGTEAAAFSTIDSATHRRRRGALNPM

FSRRAILVLEPVVREYVALLCKRVAEYATKSKPLAITVAHSAYTGDVIV

EYSFGFSYRHLENPNFESFHDALMVMGLSAHLASQFSWFLP (0)

QLVNSLPDWFVEKIQPDPASLLRLRR (0)

DHWDLIGQIIRGEEAVSKVKLHPTIFHEILRSSLPPEEKSQQRLLDEAQIVVAAG VGTTAYTL &

DVGTFHILNTPHIYQRLHKEVADAFPDKNVIPSLLELEKLPYMKTCIQESLRLSYGLSG*N

PRAHDKPLQYGKWTIPANTTITMSIVDVHHDEAI &

FPDSHSFIPERWLDNPKTSDGTPLERYLVAFGRGPRACLGLS (2)

LARAELNYAFGTVFRRFEFELYETDITDVKYKHDFFNPPVKMDSKGVRLLVSEVSD

 

>CYP682M1 Fusarium oxysporum

42% to CYP682H1

FOXG_14591

MENLQLNARTLLTVAASLIALRITYTVVYRLYLSPLAKIPGPRLAALTFFYEFYYDVVKRGKFLWKIQELHRQYGPIVRI

NPYEVHINDDSFYDEIYASGPHHPRNKIRFMSTHDESMFDSYAAETHRVRRAALSGSFSKQSIRALEPQILQTIHQLAGR

MSSLRESGEVVNFKALYSGLTMDVIAQYCFGESMDNMKQDQFGKELLDFFHEMPQAHPIGRMFPWLFDIIQKIPISYLAK

LDPKLQPLADYDKKISGQISDVLAKEKKDGIRTVFHEMRDSKQLPEADKTLERFKSEAAIFLGAGTETTAAALTTLSFHL

TENPDMLTTLRKELETVTADASSPITVAKLEPLPYLLYKKV (0)

EGIRLSFGVPGRLPRYAPTEDLVYAGYKLPRG (0)

TRMSSSSYLIDTNEEHYPDPFKFDPERWMHGKTSLSRH

FVPFCRGSRQCIGMNLAYAELYLTVATIFSRFDLELVGTTKRDVEIAHDFFVGMPPLDSKGVMVRVVKDRSE*

 

>CYP682N1 Aspergillus clavatus

39% to CYP682M1, 42% to CYP682G1, 41% to CYP58E1

some overlap between CYP58 and CYP682 families

ACLA_061270

MMSSDLSSFLIHCTFKDVLLGGISLYLVYGILLCIYRLTLH

PLAQFPGPKLAAATFWYEFYYDLFPHKLRYLWKIEEMHKKYGPIVRINPIHLHINDPNYLDEIYAGGKRKRNRDPWYYRS

ESNGPLGWSVFQTVDHDVHRMRRAALNRFFSKRSVQEHEQMIIDKIEILCGRFAEACRTGDIVSLTFACGALTMDVISAY

AFGAETDNLTRQDFAAEYLEAYSKLSQLGPVGRQFPWLAKACLTLIPSSLVHRISPAAALIPRNRLFFRGIIEESVRQQE

AGKGNKGKSIFQDILRSNLPPSEKAPARLAAEANLLVIAGTETTARALSLVLFHILDNPQTVLQLREELAPLMPHPDSRV

SVAALEALRFFPAVITEGIRLSHVVSSRMPRYAPEETLRYGKWIIPAGARVMQSHYLHHMNPDIFPDPYTFNPHRWLDNP

ELKQNYFMGFGKGSRICLGINLVHAELYLTIARLMTRFDMRLHETIRERDVDVKRDCIIGLPSLEGQGIRVRITQDRAG*

 

>CYP682-un1 Aspergillus oryzae

pseudogene 99% to CYP682-un1 Aspergillus flavus

AFL2G_06952

DDDRSTLATLEQLPYLTAVVQEGLR

LCDPVTHRISRQFPDQPLQCRGIFIPANSTVGMTAMLTHLNEKIFPEPRVFRPERW

LGSDGKRLERYLVPFNRGTRSCLGMNLARAELVLILAAVFRQFDFDVSGVNRERDID

VSRDYILGAQARDTPGILVTVKGC*

 

>CYP682-un1 Aspergillus flavus

pseudogene 47% to CYP682H1

AFL2G_06952

DDDRSTLATLEQLPYLTAVVQEGLR

LCDPVTHRISRQFPDQPLQCRGIFIPANSTVGMTAMLTHLNEKIFPEPRVFRPERW

LGSDGKRLERYLVPFNRGTRSCLGMNLARAELVLILAAVFRQFDFDVSGVNRERDID

VSRDYILGAQARDTPGILVAVKGC*

 

>CYP682-un2 Aspergillus terreus

41% to CYP682C2 Neosartorya fischeri C-term fragment

ATEG_06280.1

TPISMNIWNTHYNKDFFPGPTEFWPERWMGEGTRELEKYLVPFGSGSRMCTGQNLSIAEQV

LTIATLFRNYELELYQTTKKNVVMASYCMISLPGSESPGIQVKVRKTVQ

 

>CYP682-un3 Mycosphaerella fijiensis

fgenesh1_pg.C_scaffold_32000094

45% to CYP682A1 Aspergillus nidulans

45% to CYP682L1P Coccidioides immitis

MSSISIAGIAILGYISSLTIYRLYLSPLAKFPGPKLAALTKWYEAYYEIVLNGKFSFHIEDLHRKY (1)

GPIVRITPFEIHIQDPSSWESLYVKHNKSSKYEWTAGRFGNESSVFTTSDPTLHRIRRGALNNM (2)

FSRRAILNFEPVVSRYVDIFCNGLEKFRQTGSICN

MTEAFSAFAGDVVTDYCFGFSYEHLAIEGFTHSFHDAFMAVSAFGHVALQFPWVHP (0) 

LLNSLPDSWNAAMNPPLAELLGLQK (0)

IAQVKEDTARNEKMPEHPTIFHTLLNDKSLPDSEKTNLRFAEEAQLLLGAGIETTSWSLCQTFFYVLSQPDVLS

KLKAELYEAIPNAAQLDAFDYTKLENLPYLARCIK RRICLSLSLSARKP*VLTQAFVWRDP*

TCPRLVVPSGTANSMTNARCALNNDL &

FPEADKFKPERWLGDSPKAPDGSPLERYFVAFGKGPRSCLGIN (2)

LAWMELFKVVGTIMRKFQLELYETDITDVLLEHDFFLPSPRLDSKGIRVKVVGIES*

 

>CYP683A1 Aspergillus nidulans AN9030.1 poor match 53 clan

MIFAQLPTLTAQVVHFMQSRLLLCITAFVVLCIIHTFLYAFLLSPVRHVP

GPWWARVSRIPLLYATWQRRRSRYASDLLRKYGRLVVIAPDQIHTSDETA

MKTIYAKSSIKTRFYAGMGSWKGVKSTLGFVDYRSAAPTRNNLIQCFQNR

NLDTLVDSMACHITEFCDMLKPKVSNNKAVDGVVIFRLLALDIVTDILWG

EKDTLLSKGFDQTPVFLRRFHAFSSWNALKSFIPGLDTYVRFLGSSNMRQ

LRADCNDMDVTAREALDRWHARPEERHQKDVLSMLQAMSNSDDPTKRIPS

EHIPAYMVEMLAAGSSTTSHTAAFACDQLARYPKALEALQKELIEAFPDK

ENIDERKMLSLRYLEGVIYETMRLYPMIPGPLERHLGEWIEVDGMKVGPG

VIASTAAYDQGRLPDVFPEPEKWNPERWLQATDRMKLNWIPFGHGCRSCP

GSNLALTELKYILGTLFRRFRVQLPEDYPNEPLELADVFAAGSKTGHCWL

EFEEMPK*

 

>CYP684A1 Aspergillus nidulans AACD01000005.1 revised

MQPGLSFLVGNWPSVVVLLGFAYFLTLAYGVRKNP

LSSLPGPQLTKWTDLLLKFYTVTGQRPRYVHALHQKYGPVVRISPSIVDI

SDVSASRDIHRIASPFLKAPFYKMLVRKDGESLFSTTDPEYHRRHRRLLS

SPLSDTNLRTVEPLVKARIRLAISRIREEALSPRGVADIYKWFFFMATDI

IGELSFGDSFRMLEIGKKNQYISDLETVAKIGGIRANFPWIISIGQILPL

SIFREVVVSTDRILEYANQSVERYKRHLAMNPNQPKPTLFTKLYDASLHK

EGDGECLSDREIRNDAQSFIVAGSDTTANTLTYLVWSVLKDRSIQEKLVE

ELDVL (0)

EELSDVHLRELQYMNQVINEALRLYPAVPSGLP

RVVPDKGSTLAGHWLPGGATVTTQLYSLHRDEEVFEEPER (2)

FDPSRWENPTKAMKDAYMPFGAGSRN (1)

CIGLHLAKMELRLATAYFFRSFPRARISAREDMNDGDMEMMLYFLLSPKGKRCLVEVN*

 

>CYP684A2 old name = CYP5041A2 Aspergillus oryzae

AP007164.1e 51% to fgenesh1_pg.scaffold_8000032

Fifth P450 of six on this accession

CDS complement(join(1693125..1693300,1693352..1693428,

1693479..1694095,1694146..1694531,1694582..1694720,

1694773..1694844))

MDSGFIRDNIPAIASIAVLVYVLSV (0)

VYRSQRSHLSYLP    

GPWYTKFTDLPLRYKVVTGQRPRYVHALHDKY (1)

GPVVRIGPDEVDVSDISGAREIHRIGSGFLKS

PWYSLLNRKDTQSIFTTTDPKFHNAHRRLLSSPMSELSLKSMEPLIDARVRLTIQKMQ

EEMKTRGVADVYKWWFFMATDIIGEITFGESFRMLEKGK (0)

KNQYVKDIEMISVIGGIRA

SFPILVKLATLLPLPIFKEVNASGNRVFGYATESINRYKRLLAENPENPKPTVFTKLY

NAGEEGLPDNEIRDDAQSFIVAGSDTTANTLTYLVWAVCRDPQIKKKLVDELAEIRDD

FTDEDLRSLPYLNQVINEALRLYPAVPSALPRSVPPKGTTMGGHWIPGGSTVSTQLYS

LHRDPVAYPDPEK (2)

FEPSRWASPTKLMKDAFMPFGAGSRN (1)

CIGLHLAKIELRLATGYFFRYFPNAKLSSKYDFNDNDMEQMLFFLMSPKGKRCLLEV

 

>CYP684A2 Aspergillus flavus

100% to CYP684A2 Aspergillus oryzae

AFL2G_06246

MDSGFIRDNIPAIASIAVLVYVLSVVYRSQRSHLSYLPGPWYTKFTDLPLRYKVVTGQRPRYVHALHDKYGPVVRIGPDE

VDVSDISGAREIHRIGSGFLKSPWYSLLNRKDTQSIFTTTDPKFHNAHRRLLSSPMSELSLKSMEPLIDARVRLTIQKMQ

EEMKTRGVADVYKWWFFMATDIIGEITFGESFRMLEKGKKNQYVKDIEMISVIGGIRASFPILVKLATLLPLPIFKEVNA

SGNRVFGYATESINRYKRLLAENPENPKPTVFTKLYNAGEEGLPDNEIRDDAQSFIVAGSDTTANTLTYLVWAVCRDPQI

KKKLVDELAEIRDDFTDEDLRSLPYLNQVINEALRLYPAVPSALPRSVPPKGTTMGGHWIPGGSTVSTQLYSLHRDPVAY

PDPEKFEPSRWASPTKLMKDAFMPFGAGSRNCIGLHLAKIELRLATGYFFRYFPNAKLSSKYDFNDNDMEQMLFFLMSPK

GKRCLLEV

 

>CYP684A3 Aspergillus niger

e_gw1.8.1151.1|Aspni1

66% to CYP684A2

NPLSSIPGPFLCKWTDIFVRYQTVTGNRPRYVQALHKIYGPVVRVGPNAVDIAKIAEAREIHRIGSGFLKSPVYELLKHD

NAKSIFSTTDPKFHSKHRRLLSSPFADANLHSLEPLIEARIRLTMQRMREEMTTRKVADVQKWFFFMSSDIIGELSFGDS

FRMLEQGKKDQHIKDLEIAALVGESRVAFPFIFRLAEFLPLPILREANKSRNRFGNYADECVNRYKRLLAASPEDVKPTL

FTKLYNAGKEGLSDAEIRDDASDLIIAGSDTTANTLTYLTWAVCKAPVIRQALVAEVATLPERFSDKDVQSLSYLNQVID

EALRLYPAVPCALPRVVPPQGATFSDHWVPGGSTVTTQIWSLHRDPVAFPEPEKFDPSRWASPTKEMKDAFMPFGAGTRN

CLGLHLARIELRLATAHFFRQFPRSEVSSREGMSDEDMEQVLHFLLSTKGHRCLLEV

 

>CYP684A4 Aspergillus terreus

53% to CYP684A2 Aspergillus flavus

ATEG_03444.1

MAYIQASSTQAVVVLLLVLVLTKIYRVYTGPLAHLPGPAISKWTGLVLQKHLFAGNRPRYVQKLHQLYGPIVRISPDELD

VSDSAAAKSIHRVASRFYKGRFYEHIGHRSPKTLFSSTDPQFHAYRRRLLGGAMSETSIRQHEPTVAQKVKLCVDQMARE

AERRGCIDVFKWWCFLATDTIGELSFGESFRMLEKGEKSQYSRDLELVSTLMIIRTTFPFLSRVAEYVPLPYFKQAAQSG

KRMFGYASESIQRYKKHVEM

YGDGVRPTLFTKLITKGHQEGSLTEAEIRLEAGGYIVAGSDTSAISLTYLVWAVCKN

PLIRDSLVAEVATLPEDFTDDDVRALPYTRRVIDETLRLYPAVPGALPRAVPPEGATLVD

HFIPGGVTVSTQVYSLHRDPKIFPDPDR (2)

FDPDRWIDPTTEMRDSFMPFGGGSR (1)

TCIGMHLARMELRLATAHFFRRFTSPTVSTKEGFTDDDMYQHMYFLVSPKGHRCLIDV*

 

>CYP684A5P Aspergillus terreus

54% to CYP684A2

50% to CYP684A4 Aspergillus terreus

gc boundary at DPDR, bad boundary at HLHM

ATEG_08902.1

 (seq gap upstream)

LAGRRAKYVHFLHLHM (?)

XPIVRLSPGEVSVSDISASREIHRVGGQFLKSPWYLTLTAKGVENLFNTIDPQFH

GRHRRLLSMPMSDTSLRGVEPLVNSKIQFTIQRMKGDIERTGVVDIYKWWILMASDIIGELSFGESLGLLEIGMESPFIT

DLKAVGTMEALNTTFPCLVNLASIFPLPFIPNVVSGGRRMVQYADEALARYKNHLVTNAENPKVSLFTRIYKATEDGLTD

AEVRDDALSYIIAGSDTTATSLTYLVWAVCRSPRIKQALLEEVNSLPDGFNDQDTRRLSFLGQVINETLRYAAAPSGLLRVVPSGGRVLAGHFVPAGVTVSTQAYSLHRDPTIFPDPDS (2)

FNPSRWENATKLMKDAFMPFGGGSRICIGLHLALREIRLATCYFFRTFPHAEVFDEKTTDADMEQVIYFLMAPKGKRCFIKSPGSV

 

>CYP684A6 Aspergillus terreus

54% to CYP684A2

ATEG_05259.1

MGFLTLFQSISLVLFL (0)

RLLYRRFNGPLAHLPGPEISRWTGLLGTIYWFRGQKPNYVHYLHEKY (1)

GPIVRVTPEEVDICDITAAKEIHKTGGRFLKSNFYHALAPPGTESVFSTTDPAFHSAHRRLLAMPISDSSLTGFE

QVIAGKVHLAVRRMGEEMRSRGAMDVFKWWLFMATDVIGELSFGESFRMLESGQKNQYILDLEQISSLAPVRTTFPLLVQ

LGSLLPLPVFRRVAAAGQRLIDYAQQSIDRYARLVEGSGVSAPPPTLFMKLYNAGKDGLSTTEIRNEARTYIVAGSDTTA

ISLTYLVYAVCRDERVHARLVDEVAALPENFDDRMMRELPYLNWVINEALRLYTAVPFGLPRTVPAEGAEFLGYRLPGGV

IVSTQSYSLHRDGEIFPEPDR (2)

FYPERWETVTKEMKEASMPFGKGAR (1)

ACIGIHLARRELRLATVLFFRAFPQARISTREGMGEDD

MEMKSFFLMAPKGHRCLIEA*

 

>CYP684B1 old name = CYP5041A1 Nectria haematococca

fgenesh1_pg.scaffold_8000032

Necha1/scaffold_8:72793-74540

39% to 62B1 Fusarium graminearum, 40% to fgenesh1_pg.scaffold_24000105

incorrect last exon and missing exon 3

51% to Aspergillus oryzae AP007164.1e (in CYP53 clan part D)

72793 MIHTLEGLLCRSHLAIFLALVGLIYLVSI (0) 72879

72926 LVYGRYFQLLARLP (1) 72967

73013 GPELSRYTDIILKYHWLRGTRAQYVHYLHKHY 73108 (1)

73162 GPIVRVGPNEVDISDISAVKEIHSVKGGYKKSRFYELLVPGT  73287

73285 TTNVFNSTDTEFHRRHRRILSSPLSESSIKTVELAVDQKVKLVISKMAEE 73434

73435 MAARQVTDVAKMWLFMATDIISELSFGESFGMLEAGK 73545 (0)

73603 KNQYIEDLEGLAARGPILTTFPTLIAIARKIPLPVLQKTAAAAARLRNYA 73752

73753 QHAVLRYKRDFASNPAAAKPMLFKKLFEAGEDGLSDEEIRAEAQAYIVAG 73902

73903 SDTTATTLTYLIWSVCRDMRVKQTLVSELNKLPADFDDNDLRSLPYLNAV 74052

74053 IDETLRLYAAAPSALPRVVPHGGSTLAGYYLAQDTVVSTQAWTLHRDLTL 74202

74203 FPNPERFDPSRWLEATKEMRDGVMPFGGGAR (1) 74295

74361 VCIGKHLARMELRLGTARFFRAFPSAVVSISEGMSDQDMIPKVFFLLAPNG 74513

74514 GRCLIEAR* 74540

 

>CYP684B2 Fusarium oxysporum

68% to CYP684B1 Nectria haematococca

FOXG_14626

MDSQLEGGFLRLETTGLFLLFLCTLYLLYH (0)

VTCTIFFNPLSRLP (1)

GPWISCWTDAILKYHWLKGTRAQYVHRLHQRYGPVVRVGPHEVDISD

ITAVKEIHRVKDGYRKAPFYQNLVPNTNNLFNTLDLEYHRHHRRLLSSPLSESSLKSVEPTVDDYVKTAIASMKREMDER

GTADVAKFWLFMATDIIVELSFGESFGILKHGKKNQYIKDLEGLAAKGSIRSTFPTLTTIATKLPLPIFKETAAAAQRIR

DYSAEAVARYKRYVANNPAAAKPTLFKKLFEAGEAGLSDEEIRAEAHAYIVAGSDTTATTLTYLVYSVCCRGDVRQKLVK

ELMELPDDFGHSDLRDLLHLNNVIDETLRLYAAAPSALPRVVPARGAHLAGYFIPGDTVVSTQAWTLHRDPDVFPNPETW

DPARWEKGSKLMHDAVMPFGGGSRVCIGKHLARMELRLATARFFRAFPNACVSSIEGMSQDDMELRAYFLLTPKGGRCLI

QLD*

 

>CYP684B2 Fusarium verticillioides

86% to CYP684B2 Fusarium oxysporum = ortholog

FVEG_13449

MDSQLGGGYLSLEATGHFVLLLCIVYLLCN (0)

VTFTIFFNPLSRLP  (1)

GPWISCWSDMIAKYHWLKGTRAQYVHDLHQQY (1)

GPVVRIGPHEVDISD

MTAVKEIHRVKDGYKKAAFYKNLVPNTNNLFNTLDVEFHRHHRRLLSS

PLSESSLKAVEPTVDAYVKMAIDSMKREMDERGAADVAKFWLFMATDIIVEL

SFGESFGILEHGKKSQYIKDLEGLAAKGSIRSTFPTLISLATKLPLPVFK

ETVAAAQRIKDYSAEAVARYKTNFTNNPAAAKPMLFRKLFEAGEDGLSDE

EIRAEAQAYIVAGSDTTATTLTYLIYSVCSYVDVQQKLVKELMELPDDFG

HSDLRDLPYLNNVIDETLRLYAAVPAALPRVVPNGGAHLAGYFIPGDTVV

STQAWTLHRDPRVFPDPEKWDPSRWEKGSKMMHDAVMPFGGGSRVCIGKH

LARMELRLAMARFFRAFPKAKVSSIEGMSEEDMELRAYFLLSPKGGRCLIQLE*

 

>CYP684C1 Aspergillus niger

fgenesh1_pg.C_scaffold_11000386|Aspni1

44% to CYP684A2

MESLAQLPGIFLPLAGCVLALSLSALLAVGPIVRVTPTEVDICDLPAVREIHRVRGGYLKSEWYKSLTPPGVTSLLTLIE

PTQYSEWRRLLAGPLSDTSLGKVEPMVTNHVHATIDRIASDLQSQGVSDLYKWWTYMATDVVSELSFGEPIGLLARPKET

AWVMDYLDKVGIMHAWRTTFPFVFVLGRFMPVHPFKHAIQAIGLLGKWARRSIQQYRQHIQEQPESPKPTLFTKLFKADK

FDDFQLTYLAGSYITAGSHTTAVTLLYLIYAICRDNEIRQKLLAEIRTLPENFRHDELRHLPYLNQVITETLRKYAVVSS

ALPRVVPAGGATLAGYYLPGGTTVSTQAYTLHRNEAIFPNPEKYKPNPKYSYLSMLSVWLIECRFDPSRWESPTQEMKDA

YMPFGGASRMCIGNSLALMEIRLTTTLFLRRFPEVQMSRQNGMRDEDLAQEQYLIMAPRGHRLLVEA*

 

>CYP684D1 Mycosphaerella fijiensis

43% to CYP684A2 Aspergillus oryzae

e_gw1.8.467.1

MALTLLITIIVAFVS (0)

WKTYTALTNPLRHLPGPWISKWTNLRFQWAVMTGERASYEHALHQRYGPVVRIGLGEVSLASISAAKIVYQIGSPYHKPTWYG

TATGFGDMALFTIRDPKAHGVRRRLLAHNYSENWIKQMEPYISEKTRVAAKKMVADLDARGYTDVKKWFTFMATDIVSEA

AFGESFDTLQSGEKTQYIIDLENDGLRGFIRAELPLLNKLVAYLMFGSANEVSAGLERMKNYAEDRISRYFEAYRADPDH

VKPTLMSKAFSAVEDGTLPKVAITSDAMTNIVAGTDTSAITATYAAWYLSQDPDLENALTEEVAMLPQHFTEENLIKLPL

LNGVITETLRIRPPVGQGLPRIVPDGGADFEGHFVPGGTTVAIPAYAMHHLPQIWRDADKFDPSRWNDATKDMQSAFLPF

GGGSRVCIGQHFAMLEMRHALASFYRTFSQGMRPAWVDGFTADDMKPVSFFVTAPKGQRCLLAKRQRC*

 

>CYP685A1 Aspergillus nidulans AN8408.1 28% to 613A1 613 clan

aspyridone biosynthesis

Nature Chemical Biology 3, 213-217 (2007)

doi:10.1038/nchembio869

Genomics-driven discovery of PKS-NRPS hybrid metabolites from Aspergillus nidulans. Sebastian Bergmann1, Julia Sch�mann1, Kirstin Scherlach1, Corinna Lange1, Axel A Brakhage1,2 and Christian Hertweck1,2

MGFVNTLTQASDQENQRLRASPQVFKLFVLILFVLLVLKIRRHRANRITN

QYGRQICELHGDARVAKFAFSKQLSDQGKALAGDEPFIIRNGRARELVVT

KPEHIYDFYKGDTK (1)

LADHPKPLYLNMGNYFSG (2)

LLGHAVGALAGERWSMIRRYFDPAFSFQSARQA

IPELSASIDRWLDDLPLQGTGTGKGFALEIKKPCRFLPLRLAAEFVYGEI

FDDKLFSALLDLNVLHEVILHDVIANKRLATRLGCWFDRAAAKRMEEFRS

RWMEFNLGIIQSARGASKACPAERIYHGVEKGDLKLEEFLHTLDEILFAN

VDVSSAVLGTLFEHLAVNTAFQQKLRAEIETHIQTRTHTPDTDSDIDINT

ETGKYLSKQDTLMNFAVMEAMRLSPAF (1)

AFSLPECTAVPKEIGGYR

VPARCPVVIDAKRLNADRATWGKDGDTYRPERFRDIPPSKSRYGFMRFGV

GAASGRCLGKHLADTLFKLTLIAVIERYSLHSVHDGPEVELREVVVRV*

 

>CYP685A2 Neosartorya fischeri

85% to CYP685A1 Aspergillus nidulans

NFIA_001570

By comparison to 685A1 with no intron at PSSK/LRYG a gc boundary is predicted

Note: this seq does not have an ortholog in A. fumigatus

MGFVDTLTQASDLDKQRLRTSPQVLKLFALILFAVLVLKIRARHRADRITNQHGRKICELPGDARVSKFAFSQQLSDQGK

ALAGGKPFIIRNGRARELVVTKPEHIYDFYKGDTK (1)

RPADHPKPPYLNMGKYFSG (2)

ILGHAVGAIAGERWSMIRRYFDPEFSFQS

ARQAIPQLTASINRWLDDLPLQGAGTEKEFALEIKKPCRFSPLRLAAEFVYGEVFDDERFSALLELNVLHEVILHDVIAN

KRLATRLGCWLDHKAAKRMEEFRAKWREFNLRIIQSAREASQVCPAERIYRGVEEGDLKLEEFLHTLDEILFANVDVSSA

VLNTLFEHLAANAAFQQKLRGEVEAHIQSRTHTPDTDTETDTNTHTGKYLSKQDTLLNYAVMEAMRLSPAF (1)

AFSLPECTAVPKEIGGYRVPARCPVVIDAKRLNADPATWGKDGDTYRPERFRDIPSSK (1)

LRYGFMRFGVGAASGRCLGKHLADTVFKLTLMAVLERYSLHSGQDGPEIELRYMNQKE*

 

>CYP686A1P  Aspergillus nidulans

           GenEMBL AACD01000154 AN8510.2

           Revised sequence, now a pseudogene

           pseudogene two stops and two frameshifts

           33% to 613A1 613 clan? Similar to CYP5082 family

MAAVQFPIEADPQWPSNGTSPVQSLPGPFNTRF*ELFHHDQPQPPRI

PILALTARYRAVDRKRSLTGPDYVWPFGNFRHMFAESRALSHKW

RKEYGDIYRIWTGFYSEV (2)

VISTPADIATFYSDSASHKKTLALRSN*VVNKLLGACVGFTNGX

RWKRNRKAFDPLLANNVAVKHNSRSHQEAAEFVSSIATTRVDG

GSNEETMIIKAAGSFLPFTLNETTSIFFGPLTDEEKIELWDVCL

AFMGIVDTALLKG

PFRWGLGYWMLSREKYQSAKAYLARWEAFLMRTSQARKDQTDLPITR

LWRDVEVGNLSKDE (0)

LLQTLTESTVFNLEPTSHALIMTIFLISDNQAFQDNLVAELDANRSAITRYLGRKDTLL

HYALLEAVRLQPVL (1)

PFTLPEAASVDKGLGGYLIPKG (0)

TSVIADSYGINVRNPFWGPDATSYRPSRFTTLTPAEIHRNLSMFWFGARKCLGQFFA

KQVRAVVFNLFDRYKVTCSDYSGIAPGINVDKTEYVDRFGLEFLL*

 

>CYP687A1 Aspergillus nidulans AN9335.1 revised 7/18/07

MEGVVTMTEARTQSNVSVSDFSLVTDN

NPEKIYAQFAELRSRCPVARTADNGGFYLLTRYEDIKNAASNTEIFISSV

KAVIPSDPRGIRRPPLNTDPPAHTPYRTALDRTLRPKRLRRLEPVLREHA

EREFAKLVAKGGGDICGDFAAIFAAWVETTWLNLEDGTAPMLANTAAAWV

NAWRRQDAKETSEQSEKLYLIARELFADRRVSPRDPEQDPASSLLQERDT

NGQPLKDELLIGALRQSLVVGMVAPPLLFGNICNHLSRDKELQNRLRSNP

SLIPAAIEEFVRLYVPYRGFCRTPSRDVELCGRTIPAKSPITMTYAAANR

DPSIFSQPDEFILHRPNITSHLGFGRGRHRCAGMPLARMALQIGLVVILQ

QSSDFEVNGPLQYAGMPEMGISSCPLRITPRDSVEPLRQT*

 

>CYP687A2 Chaetomium globosum

XM_001226406.1 NZ_AAFU01001127.1

60% to 687A1

MAAAKTITCPFMATKLLSQERIDTSPAVISDNVDEILKEYDILR

EACPVAYTNQYGGYWLMTRYDDIKAAALDSDTFISSVRAVIPSDPRGLRRPPLNFDAP

NHTPYRTALDRTLKPARIKRLAEPLERHAEELLLPLLKKGEGDICTEFAAQFAAWVET

EWLNLARDTAPRLASTASAWVNAWRQRDGEKTTHFSTQLYDIARAVLADRRENPRDPE

EDPASSLLLERDSRGEPLEEIHLVGCIRQSLVVGMVAPPLMLGGICNHLSKNQELQQR

LRDDPTLIPAAIEEFVRLFSPYRGFARTVSKETVIHGQTIKPGIPITLTYSAANRDPA

VFDSPHEFILNRPNIAMHMGFGRGRHRCVGQPLAKL (2)

9538  ALQTALKTLLSKTTKFEVNGEMQFSRMPEMGIISCPLTFKIE* 9666

 

>CYP687A3P Aspergillus flavus

AFL2G_07246

Note first part is CYP687A like (65%),

Second part is CYP645A like (39%)

The CYP645A sequence has a C-term extension as in CYP645A1

this second part which occurs after the heme signature in CYP645A1 resmbles a

phthalate 4,5-dioxygenase reductase subunit

This gene seems to be part CYP687 (first 137 aa) and part

phthalate 4,5-dioxygenase reductase.  The P450 would not be functional.

This may the created by deletion of part of CYP687A and CYP645A genes

that were adjacent in the genome.

The second part is 46% to Saccharopolyspora erythraea AM420293.1.

This is a 7% better match than to CYP645A1

MFFTTTPSADEDFALIEDNNPDKIYEQFGQLRRKCPVAHTSQTGGFWR

LTRHDDVERAASDPDTFVSSVKAVIPSDPRGIRRPPLSTDPAAHSPYR

TALDRTLKPARLKRLAPILERHAQRELDALLKTNTVDHQDA

(deletion)

KIHYEKVRPARGVAGVVVAWDPPLAPSLRTLPHGSSTEMASSTTQNMSAKVLSLKSMT

SEVLGVTICSNTPDELPQWTPGYHVDILSQYGYRQYSLYSYPAETSFWKLAILKEENGC

GGSKWLHENLREGMDVTVRRPKNHFRLRKRPRYILLAGGIDITPLTPMLSELK

CNGADYHLIYLGRPRGTMAYVEELCRGHPTEVWTSQDGKGFDLESFVKAQEKNVQ

IYCCGPDRLINALEDACRENVVVELRVERF

NKSFHVTLGRSGRRLTVPPEKTLLEVLNQNGCGIMSTCSK

GTWGTGEISVLEGRPEHRDTVLSPEEKGKNKVMMPCVSRYLEEELVLDLW*

 

>CYP687A3P Aspergillus oryzae

Supercontig 25: 1280400-1280807 (-) strand

51% to CYP687A3P Aspergillus flavus (best match in A. oryzae)

possible ortholog, the reductase part is not found in A. oryzae

This gene seems to be undergoing some kind of active decay.

Other P450 pseudgenes are still 98-100% conserved.

Adjacent to VIII left telomeric region

MFFTTTSSADNNFALIKDNNLDKIYKQFD*LQRKYLVVYIF*TGDF*LLIYYNNIK*TIS

TTNIFILSIKVIILSNL*SIYRPLLNIDLLVYIFYYTALNRTLKSARLKRFGLILKRHTQ

RELDVLLKTNNIDNLD

 

>CYP697A1 Heterobasidion annosum

AY827553

MAYTPGVIDALLAVVAVLVIKTIAQKRSSSPLPRGPRGWPIIGN

VLDMPKRSAWEVFSNWGDKYGNLVYVSVLGQPVIIINSVKAAVDLLEKKSSKLLGPPA

VPNGPSHRLGRGPHLHGVRQHLSPSAQASPPRHWLARAHAELPRSHRERVHAFTQRLM

KNPEDKTELIKRSTTAISMLMAYGYEIKEENDPVVKLAEQSVEDLGHLLESGNFLVEI

FPWLKYVPEWFPGAGFQKTVEEWKTTLDTIQRTPFEFTKAQVAAGTAIPSVVSEYLDK

DGGSSEDEVAVMRTAANIYGAGSDTTTSAISTFFLAMTLFPDIQKRAQAEVDSVLGTG

RLPTLRDRDNLPYIDAVVKELLRWRPIFPLAVAHRLSGPSDDVYDGLLIPKGAVIIPN

TWRFMRDPEVYKNPLEFNPLRFLPEDGSEPELSPYGFVFGYGRRICPGVHMADAVLFN

MCSMVLSLFNISKAVDSNGVPIEPSTDYEGEATRRPMEFPCIIKARSKTAEQLILGS

 

>CYP698A1  Penicillium paxilli

          GenEMBL AF279808

          Young,C., McMillan,L., Telfer,E. and Scott,B.

          Molecular cloning and genetic analysis of an indole-diterpene gene

          cluster from Penicillium paxilli

          Mol. Microbiol. 39 (3), 754-764 (2001)

          Gene name PaxQ

          note TG boundary at intron 1 not AG

MDFVLSALQRDSWGIAAIILVSIWALHSFHRSRKLQ (0)

IPVPYVGKCGILGPWISALQWESKARELVQEGYEK ()

HGNFAFKVALLNRWEVCICNEDMIREYKNLMDNQFSAIAVTSE ()

LFQIKWTAPGTEEGAHKISIPLLGKALTWQRNRSAAQNDPYFSEFV

EEFLYAWKEEVPVPENGDYELPCFETGARVVAHLTARSLVGYPLCRNPEIVNLFTDYG

SAVPTSGFFIAMFPEIMKP

FVANFCSAPRISKRLQAILLEEFAKR

REEGGIEST ()

DIMGWLRNWTDQNEPGVYGDLEITSSIIATIFGAIHTTTQ ()

VLVHCLFELATRPEYVEPLRVEIQSA

LEEHGGWVKEGIEGMVKLDSFIKECQRFNPLDAG

SLARRATKDFTFKNGLTIPE ()

GTFVFAPNGPILFDDTLYPEARQFDGYRFYNLGQKTGKPQDFRFAATNQKYLQFGDGRH ()

TCPGRWMASDEIRLMLAHILMNYDIATKDNKGRPENWIFKKILFPDMKAVVILKARK

SVSA

 

>CYP698A2  Aspergillus oryzae

          GenEMBL BAE65190.1 AP007171.1

          61% to CYP698A1 with some assembly errors

          698A1 is from an indole-diterpene gene cluster

          note the other P450 in this cluster is CYP653C2

          The ortholog of 653C2/paxP (653C1) is 4 genes downstream of this gene

          14 P450 genes and 2 pseudogenes on this contig

          probable GC boundary after VTAE

          frameshift after VETIL

1893711 MSRLLERTLDRFTGLLEYQPTYPFAAPTWVYLVGAILIQQLATRWYRYYKSW (0)

VNVPVVGGHGIIGSWIAAFWWTARARSLVNEGYQK (0)

HGDFAFQVSTPTRWEVFICNDEMVREYRNFTDERFSANAVTAE ()

LFEAKYTVPGAAEGVHKVPVPIVAKALTWQRTRAATKTDPYFEEFVKELQHAFDAETKFENED

WNDLCCFATGTRIVAHLTAKSLVGYPLSRDTELINLFAEYGNAVPTSGFFIAMFPQILKP (2)

FAAKFCSAPKISARLDRIVMDELRKREANPRSEPQ (0)

DITDWIMFWSRTYPGTYTDQDIARSVVSAVFGAIHTTTQ (0)

VLVHCLMDLAIRPEYIHPLREEVETIL

IGDNQQWTKEGLESMEKLDSFVKECQRFNPLDA (1)

GSLARRATKDFTFSKGLHIPE

GTFVFTPNSPVLFDEKHYPDAQQFDGYRFYRLGRVTGRPLEYKFIAANLKYLQFGDGRHIC (2)

PGRFMAADEIRLLLAHILVNYDIRPKDDGERPPNWTF

KKILFPDMKGMVQLKRRSINISQPN* 1891669

 

>CYP698A2 Aspergillus flavus

AM921700

97% to CYP698A2 Aspergillus oryzae = ortholog

complement(join(11445..11634,11697..11940,11996..12176,

12236..12352,12397..12508,12562..12929,13003..13119,

13172..13276,13329..13484))

/gene="atmQ"

/function="aflatrem biosynthesis"

MYRLLERTLDRFTGLVEYQPTYPFAAPTWVYLVGAILIQQLATRWYRYYKSWV

NVPVVGGHGIIGSWIAAFRWTARARSLVNEGYQKYGDFAFQVSTPTRWEVFICNDEM

VREYRNFTDERFSANAVTAE (0)

LFEAKYTVPGAAEGVHKVPVPIVAKALTWQRTRA

ATKTDPYFEEFVKELQHAFDAETKFENEDWNDLCCFATGTRIVAHLTAKSLVGYPLSR

DTELIDLFAEYGNAVPTSGFFIAMFPQILKP ()

FAAKFCSAPKISARLDRIVMDELRKREANPRSEPQ (0)

DITDWITFWSRTYPGTYTDQDIARSVVSAVFGAIHTTTQ

VLVHCLTDLAIRPEYIHPLREEVETILNRDDQQWTKEGLESMEKLDSFVKECQRFNPLDA

GSLARRATKDFTFSKGLHIPEGTFVFTPNSPVLFDEKHYPDAQQFDGYRFYRLGRVTGRPLEYKF

IAANLKYLQFGDGRHICPGRFMAADEIRLLLAHILVNYDIRPKDDGERPPNWTFKKIL

FPDMKGMVQLKRRSINISQPN

 

>CYP698A2 Aspergillus flavus

98% to CYP698A2 Aspergillus oryzae

59% to CYP698A1

AFL2G_05467 revised

MYRLLERTLDRFTGLVEYQPTYLFAAPTWVYLVGAILIQQLATRWYRYYKSWV

NVPVVGGHGIIGSWIAAFRWTARARSLVNEGYQKYGDFAFQVSTPTRWEVFICNDEM

VREYRNFTDERFSANA (0)

LFEAKYTVPGVAEGVHKVPVPIVAKA

LTWQRTRAATKTDPYFEEFVKELQHAFDAETKFENEDWNDLCCFATGTRIVAHLTAKSLVGYPLSRDTELIDLFAEYGNA

VPTSGFFIAMFPQILKP ()

FAAKFCSAPKISARLDRIVMDELRKREANPRSEPQVQ

DITDWITFWSRTYPGTYTDQDIARSVVSAVFGAIHTTTQ

VLVHCLMDLAIRPEYIHPLREEVETILNRDDQQWTKEGLESMEKLDSFVKECQRFNPLDA

GSLARRATKDFTFSNGLHITEGTFVFTPNSPVLFD

EKHYPDAQQFDGYRFYRLGRVTGRPLEYKFIAANPKYLQFGDGRHI

CPGRFMAADEIRLLLAHILVNYDIRPKDDGERPP

NWTFKKILFPDMKGMVQLKRRSINTSQPN

 

>CYP699A1    Agaricus bisporus (mushroom)

            GenEMBL AJ312129

            Eastwood,D.C., Kingsnorth,C.S., Jones,H.E. and Burton,K.S.

            33% to CYP502A1, 34% to 620E1

MPILSLARDILFISLCGLIYRIFRNRRNRLLPLPPGLLRWPILG

NALSMPLTYAHIYYKHLGNKLGSKFIYLEAVGQPFLIINDYRVAKDLLEERSAIYSRP

QFPMSTEVIGLKRFFLLMPYGDIWRNHRRVFRQYFDPKYLPRDQEKQLNFIRKSLLPN

LFIAPEEAHEHVANCIGGFILSIVYGLPVKTRHDRLVRFAEKTFNDLAASTAPGKYLV

NIIPQLQYLPDWFPGTEFKRVALKTAEELDKLREEPYQAVLIAMVGHVVQNCLVSDNV

EKLRNAPDFEDRIVDVKEVARTIFGAGFETTTGVIMTFVLAMLLNNDVQLQAQSEIDA

VLGPERLPTFSDLSDLPYFSAVIKEVLRWNPIGPIGIPHATTNEDEYDGYYIPKDCIV

VSNIYAMLHDENIFPDPEKFDPGRFMKNGTTINDDLLNPIDVVFGFGRRVCPGSHLAM

SMLEITAASILCLFDILPALDAQGKPIDVVPEFTPASITSHPLPFKFNVSPRKGKDVE

GLLSEYMNVEYA

 

>CYP699A2 Agaricus bisporus

partial mRNA for cytochrome P450 (cyp gene).

AJ271708

84% to 699A1, 42% to CYP502 Coprinus cinereus AB013443

ENVEKLCNAPDFDDRIAEVKEVARAVFAAGFESTTGNIMTFILA

MLKNHDVQRQAQSEIDSVLGIERLPTFSDLSDLPYFSAVIKEVLRWNPPVPIGVPHLT

TDEDVYDGYYIPKSCIVMSNIYAMLQDETVFPDPEKFDPGRFIKNGDMVNDELLNPKD

VIFGFGRRVCPGSHLAISMLEITAASILCLFDILPALDTQGKPIDVVPEFTPASITSH

PLPFKFTFSPRKGKDVEGLLSEFMNVEYA

 

>CYP5025A1 Ustilago maydis

GenEMBL XM_404078.1 in the CYP52 clan 39% to 557A1

MSLKVQLPHSSLCRIGQRTTGAQGGLSSDKAAQKEKVGPLKSGLLFSYMQNFCALQTRLRRPSRTTE

LDTMDFKPFLTLQHFRPQGFAGDVLAPGASYNQTWNTMASKFNGRGGNRVETGKVLEAASESLKETV

PLLQLVVRARHHPLLVFLVGLFLGTIYLLYRYWDCAVGCERRPDLKGPKGLPLIGNLMWALKNRDPL

SYQVYAQQKYGYGNTHTLPGLGRLIDISRPDWIEHVQKIKFSNYVKGEQFHDQMRDVLGDGIFTSDG

ERWKMQRKVASRIFTVSSFKAIITQTIREDCALVEQLIETYARQGTVFNLQELYFKFTLSSFVKIAF

SQDIKSLSEPDRPDTFGDAFNYAQKVLDMRFVQPWWKIAERFNETGRKMRAARKIVEEFTTNIVEAR

RKESEAMGEKSKPESSRKDLLDLFMAYRSSDGQRLSNQQLKDTILNLMIAGRDTTAEALSWMSWHML

TKPDVYDRIRHEIDATLEEEGEQAGLEIDYDVFEQHTAKLTTFQETLRLHPSIPKNIRRALQDDVLP

NGGPRVRKGDLMLYSDWAMGRNPDIWGPDACEFKPSRWTDQETGSSIKYSQFQAHFFNGGPRLCLGQ

KLASYEVVQLIHHIFAKFDLELIDLGPGRSAGFGKVPDYLNSLTHPMKRPLMVKATLRCCKEGTR

 

>CYP5025A2 Phycomyces blakesleeanus (Zygomycete fungus, Mucoromycotina)

59% to CYP5025A1 Ustilago maydis

e_gw1.3.736.1

MSPKEEILLLDDSENILNSRASIQNQQLVYVDLKDHIKHTIPGPLNIPFVGSLYEMLPNITESWVRQFEK

YGPLVSVSILGTEMINTNNVDIAEIFSKESEYFTKRIKGSVLSEAKRVGLQGLFTTDTDETDWRLAHQLL

MPAFSSRAIKAYQIEMGRLVQQTIRIIEQIPQDGPVDIIDLSTKLTFETIGRIGFGYKFGLIDSLNSPSH

PFIGAMSYCLQQAVVRTQQARFVRSLPTEANRTFDRSLKLMHDIVDKVIQDRKRVPKSDTDNSESDSDSD

SNNNKDLLDFMLYARDENNLGLSDENMRDQVVTFLIAGHDTTANTVAWALYEISRSPEVEAKIVQEIVNA

GITAEELPTSEQISDLKYITQVIKETLRRYPPIRIISRICKKNCVIPGGYLIKKGSSVGVHLYSLHHNPE

IYPNPYRWNPDRWTPEEEQKRSRYSWMPFSTGPRACIGMAFSLQEMKTSLAMLLQKYRFIYDGPPIQFDP

KMATTKPYSFMMTVKPRTSFPQSNVSLK

 

>CYP5025B1 Ustilago maydis

GenEMBL XM_404088.1, EAK87284.1 in the CYP52 clan 38% to 557A1 if inserts removed, 42% to 557B1

MISLVQHSFDFFETRLGLAVGFIITLALVYRDRAVFVAKCDHIKGPPGLPLLGNTLQVFKEAADPLQ

AMLSRSAVYGLPNTMTVLGMGRAINITRPDWIQHVQKTNFSNYVKGPKLHTCLKDLLGDGIFNADGH

SWKAQRKVGSNIMTMSNLKNLVAGVLDAQSQRFSDLLQQAAKQGTPIDLQKAYFDFTIQTFLRIAFS

TDLETVQSTRIATRSEQQKAAQHLSFADAFDLAQRLTVRRINRPWWKLTRRWEPSEKKLEHAIRTID

RYLYPLIERRSQNKSKADKRHADLLDLFLSYRDDQDQPLTPKQLRDALLNYLLAGRDTTAESLSWAS

FELLRHPEVVHDLRQELMSHSIWFDPHQKIDDDDAEQRSSSFELSHTKQLHHVRSIYHESLRLHPSV

PRSSKIALQDDVLPGGVVVPKDTTVIWSDWLLARNKSVWGDDADEWKPRRWLNAEGEFINESPWKFH

AFNGGPRTCLGIQLAQFQGMYMLSRIFSDFDLELESDRGLHFEPEVENTLTLPMKHPLMVRVYTRAK

TSRAP

 

>CYP5026A1 Ustilago maydis

GenEMBL XM_399338.1 in the CYP52 clan 45% to EAK82744.1 36% to 557A1

MPAFPSSSAANSAGRHIELRSDNAYVQHLVHVINTVLERTGLALPVLVLTLLFLTIKYHNRTIFAKP

PRKELYSPPAWPLIGHTLQAMKYTDNILDWFLEQSRKHPVGFSASIAGTGSGQMNFIYRPEYIEYVQ

KTNFENFEKGQAVKTRFSDLLGQNGIFNSDGHVWKTQRKMASHIFSVYQFRTWVQTVVQREMDSVVG

ILDALTEGKSSSDPATLLLPDLFFRYTLSSFGKMAFGSDIDCLSADPSCLEREVEFSNAFDFVQMVI

DKRFRKPMWQLIERFSSEGRKNARMVKKINSFSGQIIEQRLREREAGIQKEKMTSDGKEGKDLLDLF

MDITTDRDDLISVVLNFIIAGRDTTAQGLSWLFYELMKNPQYVEEIRSEIRNVLGNTDGSIRKLDYD

TTKDLVFTQACFNEAIRLHPPVPKNGKRAIKDDVVVPQGPTAAGLPPIKIYAGELLSWSDWVIARNP

EVWGPDCCEFKPMRFIETVTNEDGTKTRSLKNFGQWKFHVFNGGPRLCLGMTLANYEALSIIAAVMN

RYDFGWGTKAQGQTADWPLKYLPSVTHPSQMYTATVVRRDELM

 

>CYP5026B1 Ustilago maydis

45% to CYP50026A1 GenEMBL XM_399478.1 in the CYP52 clan

 

MSILLPTVHSSFEHLTPTHVAIASVLILGLLCLIAPDRYIFVSHRKGIKEVPGALPLIGNLLFLLDI

AAKRRRFLDEFLRMQKSIGAGGMPWAATFPYLGGRVTSINRPEYIRWVQKTNFENYIKGSFFQKSMG

DVMSLHGIFVADGEIWRKQRKMASHIFSVGNFRTHVQNTIHRDLAILNKLFRDASSKNTELNLPDLF

FRFTLSSFSLMAFSADIKCLPSNVGDLSKVVEFAANFDYAQRVMDERFVDPLARFTELFSPQGRKMR

RTIKELHSFCYEIIDLRLAARARGEAQAASGKGDKDLLALFMEQNLTRDELLPVVLNFLIAGRDTTA

QALGWLFYELSKHPECIEKARQEIHEKLGSGSSFQSMAYDDLSSLVYVQACFLEALRLHPSVPKNIK

LAVKDDVIRPYAQPCSDADVAPNAVPTTQKLPDLVIKKGETVTWQDHTMARMPELWGEDCEQFKPER

FIEERKDGSIGIKTYSQYLFHAFNAGPRLCLGQTLATYEGCAVVAEILGNFDVVYDHKALKEDEPTY

DDSLTLPIKNPYTIHVRARDD

 

>CYP5027A1 Ustilago maydis

37% to 530A1 with some insertions deleted GenEMBL XM_401724.1 in the CYP64 clan

clusters with CYP664, CYP665, CYP530 and CYP619

MFALEVDERSKLGSLLLGSRFKLADSVLARGEWIFAATAAAAVLVLLTLRFQAGQNCQGRFKRIPGP

RQLPIIGNIGQVDAARQHPKFLEWARRYGEIYQVRFGSNRIVVLNSAKASIELLDRRSSIYSCRQGP

HFAHDLMSAGQRQVFLPYSKEWKAARASVHPFLMGTKSQGYKTIQEHESAVLIHDLLRHTQHCLSHR

RDQDLPLTHETGWEAHVRRYTTSVVMNITYGRRVTSTYRNPELHKIYDVLGNLAVVGQPGRNACDAF

AFVRKLPDWLAPWRVQAKAMHKWEMELWGGLLERTKRDRQHGRPCQGYVASVLDRRQHAPSSASFDG

SGLTAEGEMTDLLLAYTAATVLEAGSDTTASSISFFILACLNNPRIWDKLFKELISACGDALPTFDK

IQDIRYLRACIKETLRRRPPTIMGIPHRVIRDDVYDGYLIRAGTVVMGNVWAIHNDPQRYPNPEKFE

PERFLGDDLSAAQSAATMDAERRDHYTFGWGRRVCQGMHIAENSLMIVLARLVWCFELSTPVSSDGT

TPIAVPSIDQEANFLPGFVSQPKAFACHFVPRSDHVVQTLENAAAEAQEYWSANDLEPDQRDHYYKD

 

>CYP5028A1 Ustilago maydis

also named CYP5079A1 by accident

37% to 532D1 GenEMBL XM_399810.1 in the CYP53 clan part B

MTTAPHTGIVESSVVATLAKILLTPLSGWPSMLQLLTGSFVLLFGWIVYNLAISPLSRYPGPLSARLGIPFWRFWHSLQGDYAPALYSLHRKHGDVVRIGPTHLSFADPTAMNSIYSLSDGVSSKFRKSNFYRSFQVFKAHPSIFSERDPHQHSKRRRAVASAYSMNSLVKLEEYVEDVTELLLKRLDELVETNSVVEGGGTSEKTKTKAVRLDPWFHYFAMDVVGELAFGQSFNLLHRGKDDERFLTGVLNLSQWGAVAGCMPLLSSLILWLFPRITGQPGGSVIGDKTTSRIQTRYAEVQDNGGVASRPDMLTKFMEARDPDTKEKYSMKQVLFSASSVMSAGSDTTATSLTIFFGYLLEHPACYAKLRQEINEAIQAGHLISPVKYAKGAQLEYLQACIKEALRLSPPISMDLPRYVPAGGALIGKNPHVIPAGTTVGISPYVLHRQPEAFGADAEMFRPERWLEGGEEGRKAMERFNMTFGGGSRACIGKNISLMELSKTIPEILLRYDIKKPAVSTHLHTTPSNAKQPFFQGKSAWFLEAHVSHHRFHNFE

 

>CYP5029A1 Ustilago maydis

GenEMBL XM_403406.1 38% to 65M1 C-term in the 53 clan part A

clusters with CYP671, CYP628, CYP670 on trees

MAQTAMQSFEQHRFLCIAVLSVASHHLIFRHVELWPMQLLALTTALFSIDVSLRHSVNKTTLFPDAA

KLAYVDLAVWTLGVLISIGVYRLFFHRLRHFPGPKAWAVSKFALMTTDAKGLRPRALEEAHKKYGDV

VRTGPREISINAPTAIAPIMGAKSSFWRGPWYEATAGARGAHVPRNLHSVLIESDHSARRKIWDAAF

SAKALKGYETILVDNIDNMVNQLEKRSQRKETVNIDDYCSFYSFDVMSQAGFAGDFGLLNHGQLTPM

IQALEDFMSFVQLVGNLPYLVEVFGLLPNPIAEFDKYMTRIVMERKARKEAVPDIMGHLLHEVETEK

GKSSKRQDAEATTDARLIIVAGSDTSSTTMGIAMFFLMEHPKILADLRQELLDVFGDDASLLTDFAR

MDDKTCPLLNAVINESLRIFPPVPTGLQRESTSPAVVDVNGQKVVIPANTIVTLPIWSLQRDARNFS

PEPLRFRPERWLHPENEEHFNKTAFTPFSFGKTSCVGKVLAYMEIRLVISNLVRRFDFVPTGNYDAQ

KFQDSLVDAFVTKRNHKLPVHVKVRNHVGP

 

>CYP5030A1 Ustilago maydis

also named CYP5072A1 by accident

GenEMBL UM06459, XM_404074.1 43% to 576A1 C-term in the CYP53 clan

clusters with 576A1 on trees

MAKLSRLDPCSRIAVYVERTHGLLLEVQQPSNTKRLDLLASLNPHLNAPPSAAKMLNETIFGRQVADVREHGLFGRPFWLNALYAFLLYSAFLGYKSYFLHKTSRIPGPWHLHWTMLPALFYYVRGTQWRYVDKLHEKYGPLFRLGSRQIYVSDKDAIRQLLAKENLPKVNWYASLSRDPKTAGMFTTVNKEYHRSRRRLMSPAFAVEFTRQLEPFLVDATTKLFEGYHQRVQKASDPFKPLLFNVYEDLACLAMDILGETAFGVSFNLVACRDDPGADRKFADINKLLAKYLHDGGIRFFCRPFDKYMKRDLNVYKLTNPLVDARFAETEARRAAAGDAAEQVETREDILQYLVDASLEMQKGQKEKLTRTHVRDQCVELLIAGGETTSNTITYILKALLENPAKLAKLYETIEPEPLDDAVPGFTQLPETPYLDACIQEGMRMYPVTSELGRRTGKEPTVVLGHVIPPRTAISASLRALHYSPKYWASPNRYWPERFLPADSIHSASDPAPAADMEAFMPFGCGPRNCIGSKFAWHEMRMVLHTLLARYTISAVEATDKVDFRQFVTFQLAKPEYRIAVTPRMALKA

 

>CYP5031A1 Ustilago maydis

GenEMBL XM_401804.1

clusters in the 56/547 clan with CYP616, CYP617, CYP618, CYP582, CYP547

MSFEVTLSSAAEQIFALLLKPISLIRAYPITSFLASLVLVPLFLVLSAALFFFGIATWRYISLHIL

GTDMYSHIPRPARTDRYPLLTGHLGYIRKSPPVEGHLTFHRELNTKVYVYRGLFYSPRLMISDAKA

MLHMLSAANSYNYEKPASTRLVLKNFLGEGVLVAEGDVHKRQRKILQPAFSVGAIRELNPIFMRYS

RDLVEKIGQMVDRSNSNAKEGGVDGVTRPFVAQSSYAMKASKPGEPVIDIGWWCIRAALDIIGDAG

FGYHFEALKVDVDPSIVVRRAGDELGDAFNTLFKLSLKIDIVRFLQLYLSNFKWLKWVNSIPNKRK

WATDSAYGELEKVSMQIVDRKKAEIRGEMQDEIEARGKTTSSDAFTKADFDEKDSVSTNGSAPGKD

LLHLMMRANMAADVSPKEKLDDAELIGQITTLLIAGHETTSNQTAWTLWLLAQQSSVQQKLRDEIH

DHFGRGMERDPGYDELMSMPYLDAVCKESLRVNSAVPSTIRVAKTSADVPLSKPYPTRDGRGTFNS

IHIPAGREVIIPITLINIDDEIWGPDAAEFVPERWLDLPPAPRQNGLPMHLMSFISGPRGCIGNRF

ALAEFKAMLCHLVGSFHFETVQDWKVEAKQTAVIRSRVVGQEDVGPQMPLRISRIPTV

 

>CYP5032A1 Ustilago maydis

GenEMBL XM_403279.1

clusters in the 56/547 clan with CYP616, CYP617, CYP618, CYP582, CYP547

MQVKLSSSSLVSESVLGKLAATAHSHRPTTFIGYVALAIGTVVLLLGLLITYSLAKMLYRTTIYHYY

ISPLRHLPGPDPKPFLGNVEEFAKKPGAGAELDWSRKYNKDGVYRSTFLFGREAIHFHRPKALRQIL

VEDPYTYPKPALAWKLLGLIAGYGLLTVEGDTHRKMRKTMNPAFSLTNLIEQFPVYYAKIYPLVDVL

NQKVEESPEESAVIDCDAWVNKALLDIICLTAFGYDADSVNNPSNELASAYHAVTSMQNGRNQARLN

LLLNLPFGDTVLDWASDTSGWDWFLKRCKPGTAWQTFFQLLNGMHRIKNVSKEILYKQLNSGERAAQ

GGQFKAIIDIIEESYLTENDGKPKSLKQAERDMITQVLTFLGAGHETTASGVSWTLWNLATHQDIQD

KLRKECKGLMDQDDRPPYSAIKGLAYLDAVINESMRITPPVPRTIRMASKASYIDGIYIPKNTLLPI

SNRAINMDPTVWGEDADEFKPERWFNLPDKYDRTFSMITFIAGAHACIGRTMSYLEMKAVICILVSN

FKFEPVSKDQSPIMDTLITMKPQGGLELRVSKV

 

>CYP5033A1 Ustilago maydis

36% to CYP5034A1 GenEMBL XM_399595.1 37% to white rot Scaffold_7 C-term

MAISTSSRLVIHQDVLSWLQHRPFASAFTLLVVYITYKLAIKPILFPSPYRHLPRPERASYILGQRI

VEANGLTYIDASTNQRVKVSGPGEVCKHYARTLDTSVFVFPEPFGGETLFISDPFALNAILADVDKF

QSDLLRTTIIEFIVGKGIVARFGDAHRKQRKLMAPAFTPAHIKGLTPIFAKYAQLMCHKIALADDES

VDFAEYLDCTMLDIIGEAGFGYRCSALERGRGGSELSSAFNSVNQAAIDFGPARAIHLGLSAMLYPR

ASIWPLSEANRRIAKVNRVMDRITMQIVREAKSRVEKEGEDLGDKKDLLSLLIKSNLDARIGERMTD

KEISGQIQTFMFAGYETSSVTTSWTLYFLARHPEVQNKLRNILTATLSERKGIPLEELDVSTLEYDD

VWCQDLEYFDWILAETLRLCPPLSGNDRQAMQDSVLPLMTPVKMTNGENVSQLMVKKGSRLTIGIKT

VNCDRKLFGDDADEFRPERFAELPQRHAEAKLPPYATYSFFGGPKSCIGSKFALTEMKVIIIAVLSR

FQLSPEPGVTIKQHQALIVRPRVETSTGGPAAGMPLRIKRLPHQVSV

 

>CYP5034A1 Ustilago maydis

36% to CYP5033A1 GenEMBL XM_397817.1

MSTTLSNKLVRWASSHPFVAAFLALVLVSTLYLAWKRASAKPSVWLNLPGPERTSFLTGYSRDPKKK

LGVKNPTAPDREPIIGDDGVDITETPGVAIARLYDEYQCDHLVFPRPIGGELLITRDAATMNHILND

SYNYTRSTAGTKATKIIIGNGVVAVFGDEHKKQRKMLAPAFSVDSLRGLMPIFTQATAQMMDRFGKD

TSLEKRWGRDVKDSVKWFGRVTLDIIGRAGFDYDFGAVEQGPNGLAVRSTFHDAMTSTMNVSPIDAV

VGAFMFFVVPSLLYILPLTENVRKLREMRSELIKCSHKIVAAKAKQIRKELEAGVDAKETFAGKKDI

LHLLMRANMSPEIRPQDRLTDETLAGQIVTFIFAGHETSGNTMSWCTFFLALDVEYQQKLRQHIQKA

LKEMGKDGDAITELTWADVNSEEMRPLDLCIQETLRLRPPITTTFRYSTKDDQLPVTTPFKTKDGRT

LSTIPITAGQEIAISVTGNNMDPKYFGADPHRFRPDRWNNLPDLHAKSKLPSPYGSFVFNGGPKVCI

GSKFAMTEMKVILVNVLAKYRIEPVEGLKYKSVEAVVQRPAVIGFEKEGSQLPLRFVHDPL

 

>CYP5035A1   Phanerochaete chrysosporium

AY866400.1

Scaffold_9

MADTSLLSRRLKSFFDPQGSPTLLSLPDSFVVTACAAIVVHLIF

KRWEPMKLPIVAFLLFLVPACLSLLFASHLSLTKGLATAFATFYTVLVSSIVIYRISP

FHPLARYPGPLAAKITKWWHAYHVHTGKQHLYVRRLHDQYGDIVRIGPNDVSIRDASC

ISSGLGSQGLPKGPMWDGRFMYSPIPAMVGARDHAYHMQRRRPWNRAFSATALKEYEP

LIYGRVHQLVSALADRQGQVVDIAKWIGYFTYDFMGDMVYGGWTEMLRDGKDEDGLWD

VVHRGLDVSAVYGEVPWVSYYASMLPNVGKDLKRMRKMAFDRAKQRYDSGSKARDLFY

YLSNEDGAEKVTPPRPIVVSDGVLALIAGSDTTAIVTATILYSLLCNPTTYHRLQQEV

DKFYPRGEDPLNPKHYKDMHYLEATINEGLRLFPATPSGTQRAPAPGKGDRLIGKYYI

PEGTATKFHFWSIQRDPRNFSHPDTFWPERWLVAEGLEHADEPLTHNANAFVPFSFGP

YNCVGKNVAMQEMRMLLCHLMHTLDLRFPEGYVPRAFEDALEDQFGFKVGELPVIVQR

RE

 

>CYP5035A2   Phanerochaete chrysosporium

scaffold_69a

GAHLVFKRWEPKKLRVVTFLLFLVPACLSTLLLPHFGTALG

LTVGFLTYWTALTLSIVFYRVGPLHPLYQYPGPLPAKISKWWHVWHVQQGKQHLYLQQ

LHDKYGDIVRIGAS

GPNEVSIRDPACITPVLGAQGMPK

GRNMWPETAPLIGYRDPAEHMKRRKPWNRAFSSASVKEFEPIIQHRVHQLVEALSDRQGQVVD

LAEWISFFTY

DFMGDM

XXXXXXXVPWVSYYAKKLPWTAQDNKAMRVMAFSRTEQRYASGSASKDLFYYLV

SNEDGSEKVSPPRNIVIGDGLLALVAGSDTTATVVANTMYELLRHPAAYRRLQEEVDKFY

PRGEDSLDPKHIKDMHYLEAVM

SNEGLRMYPAVPSGSVRAPEVGKGGKIAGP

SYIPEGTQTRIHFWSVQRDARNFSFPETFWPERWLIAEGIEPAPAGEKLVHNPNAFTPFS

FGPYNCVGKNIALAEMKQLLCHLVHKLDVRFADGVDPDAFDRASEDRFIYVVGELPVVV

ERR

 

>CYP5035A3   Phanerochaete chrysosporium

scaffold_249a

MAGDLSTRDALGIIVVSAL 

GTHAIFKRWEIKHILVVSTLLLFLPAALSTLLIPHLGSFKGIAAGFSVYFITLLSSI

TLYRISPFHPLAHYPGPLLPKISKIYHIAKVSSGKQHLYLQELHNQYGDIVRF

GPNEVSIRDASCIMPVLGAQGMPKGP 

MWQGRHFWTEVHTLIG

FRDPKAHQRRRRPWNRAFNTAAMKEYTPLMQNRVRELGDALVARQGQVVDLAEWIGFFT 

YGFMGDVYV 

FGGWTNMVREGGDRERLWEVVKSGLK

IEFIYDNIPWLSYYTRNIPGAGNAELRAMAIGQTEKRYSRTSTSKDLFYYL

GNEDGAEKEDPPKHTVVVDGGLALVAGSDTTSSVLSSIFYCLLRHPDTYDRLQAEVDKFYP

PGEDSLDPSHLSDMNYLEA 

GLRLFPAVPSGSQRAPEIGTGGKLVGP

YYIPEGTQTRIHFWSVHRDPRYFSRPEAFWPDRWLIA

EGLQAHAAGDEPFVHNPNAWTPFSFGPSNCVGKNLALQEMRMVLVHLMHRLIVRLADGWD

PAQYEREMEDRFVFSIGRLPVVVERRD*

 

>CYP5035A4   Phanerochaete chrysosporium

scaffold_249b

MAAREAILIIALFAVVST

ISHVIFRQWEVMHCSVVLGLLVVIPAVLSTPLVSDFGIPCGLALGFTTYFAVLLLSITL

YRISPFHPIARYPGPLLAKISKIYHVSKIWSGKQHLYLQRLHEKYGDIVRI 

GPNELSIRDVSCITPALGAQGMPKGP 

MFNGRHLWPETHSLIGFRDPKEHQRRRRPWNRAFNTASVKEFNPIIQARVQELGDAFAAREGQ

VVDLAEWIGFFT 

YDFMGDMV

FGGWTHMVREGADHNGLWQLIKSGMK 

VSFVYEHIPWLSYYVKKLPGAGSDLKMMRAMAFGQTEKRYATTTTTRDLFYYL

TNEDGSEKVDPPKAVVISDGALALIAGSDTTSTVLTSTFYCLLRNPETYKRLQEEVDMFY

PAGEGSLDPKHLPEMHYLEA

GLRLFPAVPSGTQRAPEVGKGGKAIGP 

YYIPEGTQTRLHFWSIHRDPRNFSHPEMFWPDRWLIAEGLQECVGEKLVHNPNAWLPFS

FGPSNCVGKNLAMQEMRMLVCHLVQRFNFRFADGYDPAQYERDWQDRFVVMIGQLPVTIERRA*

 

>CYP5035A5   Phanerochaete chrysosporium

scaffold_141

MPSGILGQLQSLPAKLTAQDATL

VAHVIFKIWEPMQARIVSLLVIIAPLLLSTLFIPHYGT

VSGVFRSFAIYLTTLVSSIVVYRLSPWHPLARYPGPLLAKVTKLYHALMVSKGKQHVYIK

ALHDQYGDIVRI

GPNEVSIRDAACIQPLMGAQGLAKGP 

SWSGRSMFPPISPLIGIRDPAEHARRRRPWNRAFNTNGIKEFMPTIQTRVQQLAEHLGERH

GQALDLAEWFSFFTYDFMGDMI

FGGWTEMMRDGGDLQGLWTRVKAGLQ 

HAGMVPEHVPWVAYYAKKIPSVVRKVSEMRGMGISRAKMRYQQGSTSKDLFYYL 

SNEDGSEKVTPPPEVVTSDGALALVAGSDTTSSVLSNLFYCLLRDPVSYKRLQEEVDKFYP

PGENSLDPRHINNMPFLEAVI 

NEAMRLYPVVPSGSQRSPEIGKGGRAVGP 

YYIPEGNQARVHFWSVFRDSRNFSHPETFWPDRWLIAEGLQESPEKITHNANAFVPFS

FGPANCVGKNLAIQEMRLAVTHLMHKLNFRFADGFNPDEWDSQIQDVTVMQLGKLMVVVERRD*

 

>CYP5035A6 pc.42.19.1 (genewise.42.13.1) Phanerochaete chrysosporium
HLIYKRWEPLRLSVTLTLLMGVPAALSVLLIPHLGLLRGALATFSLYLSTLISSIVAYRLSPWHPLARYPGPLPARVTQLWHTWQAHKGQQHLYLKQMHDKYGDVVRMGPNEISIRDADCIVPLYGPHGLPKGPSAGRQMHPQELSLIGYRDPARHSVRRKPWARGLGTAAVREYMPALRSRVSQLVDALGARSGHPVDLAEWIAFFAYVFVSDCSLSXLANCQSGMTLWATWRECHGAGAVFEQVPWLAYYAKMLPAISQRILKMRKLTVRHATRRYNSGSFSRDLFHYLSGEDLPEGSARPPQHIVAADGLLAVVAGSDTTASALSNLFYCIMRHRDVYKRLQQEVDQFSPLGDDSLDPQHLNNMPYLNAVINETLRYLPSVLSGSQRAPLIGGGGVSVGPYYVPEGNQVRVHFYSVHRDPRYFSDPDRFWPERWLIADDRQPSSEKIVHDDRAFIPFSYGPSNCVGKGLALQQMKSTVCHVMAKLEMRFADGYDPDTWEEQVQDEGVMIV

 

>CYP5035A7 gw.54.121.1 (genewise.54.12.1) Phanerochaete chrysosporium

KSVHLIFKRYEPMHILVVSTLLLLLPAILTVPLIDQLGIAKGFLVAFATYFATLLSSITLYRISPFHPLARYPGPIIAKVSKIYHVAQVWSGKQHLYLQRLHDRYGDIVRFGGFSTCSLRGPNEVSIRDVSCIAPMLGTQGMPKGPGKHCWPEIHTLIGCSDIKEHQRRRRPWNRAFSTAAMKEYNPIIQKRLQELGDALAARQGEVVNLADWISFFTHVPWLSYYTRNIPGATNDEFRGMVFGQTVKRYACTGTTKDLFYYLNEDGAEKEDPPKPIVVVDGAVALIAGSDTTSTVLASTFYCLLRNADTYKRLQAEVDRFYPPGADSLAPDHLPEMHYLEAEALRLFPAVPSGSQRTPERGSGGKTIGPSYIPEGTQTRIHFWSVHRDPRNFSRPETFWPDRWLIADGLQKDEGVEFVHNPNAWIPFSLGPANCVGKNLALQEMRMVLVHLLHRFSFRFAGDYNPEQYEWDIEDRFVVAVGRLPVIVERR

 

>CYP5035B1   Phanerochaete chrysosporium

scaffold_247a

MGTEYVLPLLRTNILKLPTNMTRNDALLAVGGAAV 

LCHLIFKKWEPTYIPAVVTLLLVVPLGLSALLVPHYGQLLAPLVALATYHTILLTSIALY

RLSPWHPLAQYPGPLPAKLSKWWMVWQERDCKQHLYIKQLHDRYGDIVRIGPNELSIRNV

DAVAPLMGTNGLPKGPS

LRGQGLEPPITGLIAIRDPAEHARRRRPWTRAFSTA

ALKEYEPILVKRISQLCEQLASQKGTLDLATWFSWFTYDFMGDMV

RFGGGSEMLAHGDQDGIWTMFKEGGE

GQMMYHHIPWLAHYAKRLPMSPALKKMRGFALGRTAERYKKGASTKDLFYYL

SNEDGVEKTPPPAAQVISDGVLATIAASDTTSTTLSNAFWNILRHPHYY

KRLQAEVDKFYPVGENAFDTKHHSKMTFLDAVL

NETLRMYPVLPSGSQRAPFPGNGDRVVGP

YYIPDGTQARIHFWSLQRDPRYFSHPDTFWPERWLIAEGLEPAPAGEKFVHNPNAFIPFS

FGPSNCVGKNLAQMEMRMVFCYLLQNLDFELDKSWNPAERENATEDQFVLLMRSPVQVTVRRRV*

 

>CYP5035B2   Phanerochaete chrysosporium

scaffold_144

MGSEVAMQLLRANISKPFPRLDQNDALAVVVGAALV 

VCHLIFKHLEPTYIPAVLFLLVLVPLYLSALLLPHFGPLLAPVIAFTTYHTSLLTSIGLY

RISPWHPLAKYPGPLPAKLSKWWMVWKERDCKQHFYLEDLHKRYGDVVRIGPNELSICNV

DAVLPLMGPDGLTKGPC

STIGQGLEQPIPSLVSIRDPAEHARRRRPWTRAFSTA

ALKEYEPILAKRISELCEQLAQQKGSLNLATWISWFTYDVMGDL

VFGGGNEMLATGDQDGIWAMFEKSGE

GQMVYHHVPWLAHYAKRLPMSPALKKMREFALGRALERYKRGAVTKDLFYYL

SNEDGSEKIPPPPAQVIGDGVLATVAASDTTSTTIANTFWHILRYPHYY

KRVQAEVDKYYPPGESAFDTKHHNKMTFLEAVI

HETLRLYPVLPSGSQRSPVPGKGDRVVGP

YYLPDGTQARVHTWSLHRDPRYFSRPDTFWPERWLIAEGLEPAPAGEPFVHNANAFIPFS

FGPANCVGKNLAYLEMRMVFCHLLQNLDFELDKRWNPAERSRSAEDQFVLYMRCPLPVTVRRRV*

 

>CYP5035B3 PFF_271(pc.92.54.1) Phanerochaete chrysosporium

MGAGYVPQLPRSYALTSFTGTSQTHALTPVIGAALITHLNFKRWEPLNLPIVIFLPLVAPLGLSALFAPGPSYGSLVAPFITLFLYHAILLASIALYRISPWHDLYHYPGPLPAKPSKWWMVWKERHGKQHLYVKALHDRYGDVVRTGPNEISIRDVAAVVPLMGTKGLPKGTAPWGEPIVPSVVPLIGIRDSVEHARLRRSWARAFTAGALKGYEPVLTARITQLIAKLGSQTGEXIDLALVLSYFSYDFMGDMLYYGGGSELLAEGDNDGVWALGQMTCHHLPWLAKYKDLLPASPGLQKMRSVALQRTMARYKNGGLGKDLFYHLSNEDSAEKTSPPTEQLVSDGVLAVFTASDTIATVLSNVFWSILRFPRYYEQLQAEVDKFYPARADAFDTAHYGEMVWLDAITNEALRLYPIVPSGSQRAPAPGDGARVVGPYVIPAGTNARVHTWSLQRDPRCFSRPDAFWPERWLAAAGLGDSEATCEGPEGAAGAAEDFVHDARAFEPYFVGPLDCIGRALAQLELRMVLCALLQRLAFAPARGDPLERERTLQDQFVVMMRGPVMVSVSWRA

 

>CYP5035C1 ug.43.44.1 Phanerochaete chrysosporium

MSAVLNSLSPTESVLAVVTCALATHLVFNRFEPTELPVVAAALVGLPAVLSALLVGHFGLLAGAALAFATFHATLAASIVLYRLSPFHPLARYPGPLPARITRWYWARVACGGRQHLELKRLHDVYGDIVRIGPNELSFRDISVVQPMMGAQGMPKGPMWDGRTFKPPILPLVGMRDVADHTRRRRPWIRAFTPAALKEYEPVVAKRGAQLIEILAQKKHTDFVHWIHLFTFDIMSDALFGGNPGAEMMSHEDKDGIMHSMKTGFEAGQLFEHIPWLGYWMRHFPQLATATKQYRAMCFQRGMQRYQDGSSQKDLFYYLVNEDGAEKQTPDKATVIADSALAIIAGSDTTASVFSNMVYCLLKNPHAYKRLRAEVDEFYPPEENSLDPKHHSKMPYLEAVINETLRLYPVVPSGSQRAPELGTGGTLMGTHYIPENTSVRVHFWSVHRDPRNFSQPESFLPERWLAAEGLEAPPAGLSGAPQDADSAAARGTFVHNANAYMPFSFGPWNCVGKALALLELRCVATHMMQRLDVRFADGWDPAEWDAAMEDKFVIKTGRLPVVVERRF

 

>CYP5035D1 ug.50.50.1 Phanerochaete chrysosporium

MITVNRPSTQDSVIATVACAIIVYFVCKRWEPWRLAVVVPLTMLPPLVLSLPLAASVGFLNAVITTSSTFASALVAMLTFYRLSPLHPLSQFPGPLQCKISGFWMAWIVSRGKRHVYIQSLHEKYGDYVRIGPNEVSINDPTAIPQILGSYGWPKASGMSGRALHQDPLPLISLLDDAEHSRRRKSWNRAFSSAAVREYQPVVAQRATQLVQALQEQRSVVDLSQWMRLPSDIAFVNLHDFLYSFGGGTEMLREGDKAGLWQLLKDGMSTAVPFEHVPWLSHYILHFPALIRSLTDLRALAFGRSKLRYERGSTTKDLFYYLVNEDHADAEMPPMKVVISDAVLAIVAGSDTTAVTLSNIFYYLISHPDTYRRLQEEVDKYYPPGEDALNPKHYVKMSYLDAVLNEAMRLYPALPSGSMRTPAKGSGGQVVGQRFIPEGTQVRVHPYTIQRDPRNFSYPNRFWAERWMIASGNQSHHEKIAHNPDALIPFSTGPRNCVGKNLALLEMKMVTCHVTQRLSLCFADGWDPSRWWDDLEDVFVSRMGQLPVIVRSR

 

>CYP5035E1 pc.97.5.1  (genewise.97.3.1) Phanerochaete chrysosporium

MQAAHLVFNRWEPTNIAVVAALLLGVPCAAASLLFSGRGFVPRLALTAALYYACLGTSVVLYRLSPWHPLARYPGPWLLKTSKLWMVRRVKRGGQWRYIRELHQRFGDVVRIGGPNELSFCDAAMVVPVLGTQGLPKGPGLIALRDPLEHQRRRRTWNRAFKPAALQEYLPLIQKRTAQLLDALSKCEEQDVVDLGRWIRFCKYAHMPWLAQFTKHIPRVAAKLKELRAAARSRAAARYKAGANRKDLFYYLVGDSNEDGGEKEQPTEDVILSDALLAIIAGSDTTSTILTSAVYCLLTHPDVHKRLVEEVDKFYPPGADWCNTEHHADMHYLNANETLRLFPVLRDGSLRAPWVGHGDRALGPSSFIPEGTQVRVHTYSLQRDPRCFSQPDTFWPERWLVAGGLQHAEPGFVHEPGAFLPFSRGPSDCVGKGLALQDMRIVLCALLQHLELAPPRNRPFDEWKAEVDRRFADSSAVLPVSVRVRRRV

 

>CYP5036A1 Phanerochaete chrysosporium

AY866401

same as scaffold_57, PC-HN1

MDALDPRIAIPIVYFIYKKYEPSSPRSAFFLLLALPGVLVVALR

VCERFNSYATAVPTVYLAYWSLLSTFVVAYRISPFHPLARYPGPLLCKISKGWLAYVA

GKGGKAHLYVQDLHMRYGEVVRIGPNELSITHQDFTRVVLGAKGLPRGPYYDSRQHEA

GMSLDGMRDQALHAIRRRPWARGMNTAAMKYYEELIRNTLSDLIVGLKQRTNRPIDIS

EWTNYFGFDFMGQMAFTRDYGMLKNGYDKEGLLDLIEHAMQDSAWISHIPWSIPFLRY

VPGASKYWDQMKAVGERAVADRVALGSNHRDLFHYLMDEDGHEAVRPTKALVAVDGQL

AIIAGGDTTATTLSHIVYFLLRYPIYLDRLRKEIDETFPDGADSTLDFTKQTNMPFLN

ACINEALRLYPPVLAGLQRRVEPGTGGKMIGPYFVPEETQVSLFAYSIHRDPQHFSPL

TNTFWPDRWLSQEKYTLPSGDVISADEVVTNRDVFIPFSQGPMVCAGKNVALTEMRSV

MCALLQHFDLKIADQSFLDSWEDKIEETFTTKRGTLPVILSLRA

 

>CYP5036A2 Phanerochaete chrysosporium

scaffold_284

TVYVSYWALLVTYTIAYRLSPFHPLARYPGPLLCRISKAWLAYIVATSGKMHLYIQNLHMRYGDVVRI 

GPNEVSVTHRDFIGVIGPKGLPKGP 

YYETRAHKAGTSLNAIRDQALHSVRRRPWARGMNSAAMKYYEELVEETVG

DLVASLKRRTTKAIDFSEWMTYFGFDFMGHMA

FTHDYGLLKNGYDKEGLTPLIEHAMQ

DVAWVSHVPWSIDYLRHIPGVYKHWLRIKALGVQTVRKRIALGSTRRDLFYHLM

DEDNRERVKPDINVVAIDGQLAIIAGGDTTATALSHLFYCLLRHPQYLERLRKEIDDAVPIACGSF

KLDFSKLPAMPFLNAFI 

NETLRLYPPVLSGLQRRVEAGTGGRFIGP 

HFIPEQTQVSFSAYTIHRDPRYFSPLTDTFWPDRWLVQEQYVLPSGGVIPAAEVVTDRDVF

FPFSQGPTVCAGKTLALTEIRSVACALLQTFDISNADEASFDAWEDNLEEMFTTKRSTLPVFLSLRT*

 

>CYP5036A3 Phanerochaete chrysosporium

scaffold_78

MSAIFAASS

TTYIIFKRFEYSKPVPALVLLIGVPMT

LASVLRDHFAGLATAMCATAAVHWVLLTLFVAAYRISPFHPLARYPGPLPCKLSKCWMAY

LAGSGGKTHVYIDRLHRLYGDVVRI 

GPNELSVRHKDACTTVLGAKGLPRGP

YYDTREHDNGVSLDGIRDPALHAVRRRPWARAMNSASTQYFEELIQHTVSDLTGGLK

ERAGESIDLTEWMSFF

FSYDYNMLKEGRDTQGLRRMIDQSLV 

DLRWISHIPWSIPYLKMIPGASKNWDDMKAAGDKVARHRVSLGSSRPDLFHHL  

RDEEGHEAVRPQLEVVSVDGALAII 

AGADTAATTLAHFWMFMLRHPACFERLRKEVDATFARDDGPDFVKQARMPYLNACL

NETLRLFPPVLAGLQRRVGRGTGGRMIGT 

HFIPEDTQVSLVAYTVHRNPDCFSPFPDTFWPDRWLTQETYTLPTGEVIPSSDVLTRRDA

FMAFSQGPMACAGKNVALAEMRAAVCAVVQRFDLVLAYERALDEWEEVLQECFVSKLGK

LPVQVVPRN*

 

>CYP5036B1 genewise.22.99.1 Phanerochaete chrysosporium

MALLQLAQDVFRRSHPASACYALYHKYLNKPVHPLYHFCLLLVVPAALLALLQAIHRISVAQECGYALFYWLVMASATVVYRISPFHPLANYPGPLAAKISKLYLAYLTAKGRAHEDVRALHSKYGDVVRIGPNELSFNRSDAIQTIYADKTMPKGPYYVARTNLAGVVQLDGVRDFKEHARRRRPWNKAMNSAAIKSYEPIVSSTASQLLGQLSKRIHNDVNISDWMSFYGFDFMGRMVFGREWGMLEEGRDVNDYWHTMDKCLTIVSWSSQIPWSVPIIRLMKPPPEVLKMQKISDDSAMARLTSEGSGVKDLYYYLLNEDDSSKSELTRDECISEGVLAIVAGSDTAATALTHLCYYLLTHPDSLQRLRQEIEEAYPTLGSELDDLSRQAEMPYLNACINETLRLLPPVLTGLQRSVTAGSGAIIAGYFVPEGVDVSVHHYSVHRNLQDFSPIPDTFWPDRWLEQDAYVLPDGDVIGKGEVRTNRGAFMPFSVGPQQCAGKNLAMVELRAVACGLFRRFDLSLSERMNICDYEKGLRDAYTTVRGPLYVKLKPRKE

 

>CYP5036C1 ug.36.48.1 = genewise2nd.119.1.1|whiterot1 = scaf154

47% t0 5036B1, 45% to 5036A1, 46% to 5036A2, 45% to 5036A3

not in tree Phanerochaete chrysosporium

MVDRVDPRLILGSSSLVSVACYLIYKHSEPNNIPAHAALLLGVPALLVHQLGTHWSILQQGGAFVAYWALILAFTGLYRLSPIHPLARYPGPTLGKLSKIYLSYLSARGDIYRVIKGWHDKYGDVVRIGPNELSFRHVDALQPIMGTKYTVKGPYYDTRTTPEQITQMDGIRDYSVHGQRRKPWLRAMSSAGLKGFEPIVKMKALELVEELSKKVGEIIDMSEWMNLFGFDFMGHLAFGREFGLLKSGNDHDDMIRTVEDGVYGAGVISHIPWIAFLVHFPPAMKGLRAMQQMAATFARERTQKGSTTKDIYYFLTEDEGAAQSGATHDEVIADGMLALIAGSDTTSIALSHVCYFLLRHPACAARLRAEVDRAFPPGEDVLDFARHADMPYLNACINEALRLLPPGLGGLQRMVRRGTGGAMIGPHFVPEDTKLSVHLFSLMRDAREFAPLPDAFWPERWLAQDTYVLPTGDAVSKEHVTTNRAAFIPFSVGPQNCAGKALALVELRAVTCALVSKFELHKPKDYDLDQWEGDLLDLYISIRGKLPVILQARQGR

 

>CYP5037A1 Phanerochaete chrysosporium

AADS01000003.1

minus strand, 45% TO CYP5037B2, 41% TO 5037B1

36346 MGVILLNTVDALSLVLVTYVLYLIGRALSRRATRTGRQVKPPGPPGLPFVGNAYQIPHDK 36167

36166 QWLRFDEWIRRY (1) 36131

36074 GDLVHISVMGQPTVIIGSAQTASELLDAR (1) 35982

35941 VLAGSIYSDRPQAVMAGEL (2) 35885

35823 VGWDQGLGYAPGPHSPRFREFRRLFQQFMGPRAAQDSSMLAAQEKSATRLLSRLLSTPEE 35644

35643 FITHVRQ (2) 35623

35568 VTGALILYLTYGYEVDEDGFKDPLVNIAEEAMLGFARASDPGAYLVDTMPWLKYIPEW 35395

35394 FPGASFKQDVKAMRQARERLYDVPYNFVQKAM 35299

35243 AEGPVPRSFVSTYVEEKATPAFADEELIKAAAASLYS (1)

      GADT (0)

35006 TPSSLASFILAMTLHPDVQRRAQVELDSVIGESWQRLPTFADRP 34875

34874 NLPYIDAIVLEVLRWHPAVPL (1) 34812

34758 GLAHRLSQDDVYRGYYFSQGTVFWANIW (2) 34675

34624 TMLHDEIIFPDPSRFMPERYLDEHGRLKSMSRFEDPAVIGFGFGRR 34487

34430 ICPGMHFAHNSIFIAIARMLYVFNFTKAVDKNGNEITPEVEYSGFIS (2) 34290

34239 HPSPFVCSISPRSIAAAELVVQSSHD* 34159

 

>CYP5037B1   Lentinula edodes (Shiitake mushroom)

            GenEMBL AB116638

MALNVQVLTVLDLCFAVVGLIILRRIFGPKSLPLPPGPKGLPLI

GNILDMPTEKEWETFAQWGDAFGDICSVTVLGQPLIILSSAKVAVDLLDKRSSIYSDR

PVLQMGGELVGWKNTLVLLQYGDRFKRFRKLFHNLIGSHATMRQYFPAQELEAKKFLR

KVLAEPDDLQAHVRRLAGAVVLRISHGYEVQEHKDDPFVTLANSATEQFSLATSTGQF

MVDCIPALRHVPDWVPGADFKRKAKQWAATLIELVEQPHNFVKQQMLAGTASISFTSS

MLESKQLTEEEEFDLKWSAASLYSGAADTTVSAIYAFFLAMTLYPEVARKAQAEIDAV

VGNDRLPTFADREHLPYVDAVVKEVFRWNVVTPLAVPHRATQDDFYDGYFIPKGSLLI

PNLWKLTHDSRVYHDPFEFKPERFLADLLATEGLGRQVEPHPRETCFGFGRRICPGMH

LADTTVFACCVTSLAILDIDKCNENGVVIEPVNDQTSGTISHPKPFKCSIKPRSPKAV

ALVQSSDEQF

 

>CYP5037B2 Phanerochaete chrysosporium

AADS01000003.1

scaffold_164c 54% to 5037B1, GC bound at PIGPL

19304 MIRPLTLLDIALATLAVVLLKTIIARSKQRARY

19403 PPGPKGLPVIGNVLQMPKDREWLTFAQWGEQF (1)

      GNIVYLSLLGQP 19582

19583 MIILNSAKDAVALLDKRSSIYSDRPILYMGGELIGWKYILGLTPYGDRFREYRRLMAKFI 19762

19763 GGKTQVERHFPVMEQEATSFLKRILRRPDDLGANIRT (2)19873

19930 HAGAIILKLAYGYTIREDEDPFVTLADRAMAQFTEATTPGAFLVDVFPLLRHMPAW 20097

20098 FPGASFKRTAQEWSDTLNSMADVPHAFVKEQM (0) 20193

      AKDTEVPSFTSELLRDEKLQEG 20308

20309 QEFNIKWSAASLYA (1) 20350

      GAADT (0)

20465 TVSSIHTFFLTMLLFPHVQKRAQAEIDSVVGTDRLPTFEDRAKLPYVEGVLKEVLRWHPIGPL (1) 20653

      GLPHRLAQDDSYEGHLFPKGAIVIANIW(2)

20847 KCLHDPDVYPNPSDFDPTRHLSENGRSPQPDPRDYCFGFGRR (2)

      ICP (1)

21085 GLHLADTSIWITCATVLAAFNIENVVENGRVIDIVPEYTSGTIR (2) 21219

21280 HPKPFRCSIKPRSTRAEALIFSD* 21351

 

>CYP5037B3 Phanerochaete chrysosporium

AADS01000003.1

Scaffold_164d 5 P450s on this sequence

12364 MPSALSLLDFAFAALGLIIVKAFLSRTRRQGPYPPGPKGLTIVGNALEMPTSREWLTFSEWGGRY (1) 12170

12115 GDIIYLSLLGQPMVILNSAKHAIALLDKRSNIYSDRPVLVMGGEMIGWKYTLALTPYGQR 11936

11935 FREYRRFIAKLIGGPTQMQTHLPLEEHETRRFLKRLLNEPERVADHIRK (2) 11789

11728 TAGCIILKLSHGYDVREGHDPIVDLVDTATEQFSLATSPGAFLVDVFPLLRYVPAWVP 11555

11554 GARFQKTAREWRKVLERMADEPHDFVKQRM 11465

11412 AENTNVPNYTSELLQNERLDGDKEFNIKWSAASLYS (1) 11305

11252 GGADTTVSAIYSFFLAMTLFPHVAKRAQMEVDAVVGSDRLPTCEDRPNLPYVEALVKEVF 11073

11072 RWNPVAPL (1) 11049

10999 GLPHRLIEDDIYEGYFIPKGSFVIPNIW 10916

      HILHDPNHYPNPFEFDPT