Fish Cytochrome P450s are undergoing a revision to their nomenclature. Initially
there appeared to be just one fish 1A gene per species, but that is not true as shown
by Amy Berndtson in trout. Until an adequate nomenclature can be devised, these fish
sequences are listed as CYP1A, without a number following the subfamily. This does
not affect the mammalian gene designations, though it may affect the chicken
sequences.
CYP1A1 Oncorhynchus mykiss (trout)
GenEMBL S69278 (5023bp)
Berndtson,A.K. and Chen,T.T.
Two unique CYP1 genes are expressed in response to
3-methylcholanthrene treatment in rainbow trout.
Arch. Biochem. Biophys. 310, 187-195 (1994)
Note: published as CYP1A2, but it is more similar to Heilmann's sequence
than Berndtson's 1A1 (97.9% identical).
CYP1A1 Oncorhynchus mykiss (trout)
GenEMBL U62797(1697bp)
Bailey,G., You,L. and Harttig,U.
Cloning, sequencing and functional expression of two trout CYP1A
cDNAs in yeast
unpublished (1997)
incorrectly called 1A2
CYP1A3 Oncorhynchus mykiss (trout)
GenEMBL S69277 (5524bp)
Berndtson,A.K. and Chen,T.T.
Two unique CYP1 genes are expressed in response to
3-methylcholanthrene treatment in rainbow trout.
Arch. Biochem. Biophys. 310, 187-195 (1994)
Note: published as CYP1A1. This sequence is 96.7% identical to
Heilmann's 1A1 sequence.
CYP1A3 Oncorhynchus mykiss (trout)
GenEMBL U62796(2401bp)
Bailey,G., You,L. and Harttig,U.
Cloning, sequencing and functional expression of two trout CYP1A
cDNAs in yeast
unpublished (1997)
incorrectly called 1A1
CYP1A1/CYP1A3 chimera Oncorhynchus mykiss (trout)
PIR A28789 (522 amino acids)
Heilmann, L.J., Sheen, Y.Y., Bigelow, S.W. and Nebert, D.W.
Trout P450IA1: cDNA and deduced protein sequence, expression
in liver, and evolutionary significance.
DNA 7, 379-387 (1988)
Published as CYP1A1
note: subsequent analysis has shown that the 5' end of this sequence
comes from the 1A3 gene and the switch over occurs between base 271
and base 435 with base 1 as the A of the ATG start codon.
CYP1A Pleuronectes platessa (plaice, a fish)
GenEMBL X73631 (2411bp) PIR S34184 (521 amino acids)
Leaver,M.J., Pirrit,L. and George,S.G.
Cytochrome P450 1A1 cDNA from plaice (Pleuronectes platessa)
Mol. Marine Biol. Biotechnol. 2, 338-345 (1993)
CYP1A Opsanus tau ( oyster toadfish)
GenEMBL U14161 (2352bp)
Morrison, H.G., Oleksiak, M.F., Cornell, N.W., Sogin,M.L. and
Stegeman, J.J.
Identification of Cytochrome P450 1A genes from two teleost fish, toadfish
(Opsanus tau) and scup (Stenotomus chrysops), and pyhlogenetic analysis
of CYP1A genes.
Biochem. J. 308, 97-104 (1995)
CYP1A Stenotomus chrysops (scup, a fish)
GenEMBL U14162 (1566bp)
Morrison, H.G., Oleksiak, M.F., Cornell, N.W., Sogin,M.L. and
Stegeman, J.J.
Identification of Cytochrome P450 1A genes from two teleost fish, toadfish
(Opsanus tau) and scup (Stenotomus chrysops), and pyhlogenetic analysis
of CYP1A genes.
Biochem. J. 308, 97-104 (1995)
CYP1A Chaetodon capistratus (four-eye butterfly fish)
GenEMBL U19855 (2552bp)
Vrolijk,N.H., Lin,C. and Chen,T.T.
Characterization and expression of a CYP1A gene from the tropical
teleost, Chaetodon capistratus.
Unpublished 1995
CYP1A Dicentrarchus labrax (european sea bass)
GenEMBL U78316(1563bp)
Stien,X., Amichot,M., Berge,J.-B. and Lafaurie,M.
Molecular cloning of a CYP1A cDNA from the teleost fish
Dicentrarchus labrax.
Unpublished (1995)
CYP1A Microgadus tomcod (Atlantic tomcod)
GenEMBL L41886 (2497bp) L41917
Roy,N.K., Konkle,B.A., Kreamer,G.-L., Grunwald,C. and Wirgin,I.I.
Characterization and prevalence of a polymorphism in the 3'
untranslated region of cytochrome P4501A1 in cancer-prone Atlantic tomcod
Arch. Biochem. Biophys. (1995) In press
probable frameshift detected by O. Gotoh. in the beginning of the sequence.
CYP1A Microgadus tomcod (Atlantic tomcod)
GenEMBL L41917 (6837bp)
Roy,N.K., Konkle,B. and Wirgin,I.I.
Functional characterization of Cytochrome P4501A1 regulatory
sequences in cancer-prone Atlantic tomcod.
Unpublished (1995)
CYP1A Pagrus major (wild read sea bream)
no accession number
Mizukami,M., Okauchi,M., Ariyoshi,T. and Kito,H.
The isolation and sequence of cDNA encoding a 3-methylcholanthrene-
inducible cytochrome P450 from wild red sea bream, Pagrus major.
Marine Biol. 120, 343-349 (1994)
CYP2b scup (fish Stenotomus chrysops)
N-terminal fragment (20 amino acids)
Klotz et al. Arch. Biochem. Biophys. 249, 326-338 (1986)
CYP2K1 Onchorhynchus mykiss (rainbow trout)
GenEMBL L11528 (1853bp) PIR S45644 (504 amino acids)
Buhler,D.R., Yang,Y.-H., Dreher,T.W., Miranda,C.L. and
Wang,J.-L.
Cloning and sequencing of the major rainbow
trout constitutive cytochrome P450 (P450 2K1): Identification
of a new P450 gene subfamily and its expression in mature
rainbow trout liver and trunk kidney.
Arch. Biochem. Biophys. 312, 45-51 (1994)
CYP2K1v2 Onchorhynchus mykiss (rainbow trout)
no accession number
Buhler,D.R.
note: 98.6% identical to 2K1 may be an allele (5L1FL)
submitted to nomenclature committee
CYP2K1v3 Onchorhynchus mykiss (rainbow trout)
no accession number
Buhler,D.R.
note: 98.4% identical to 2K1 may be an allele (5L6FL)
submitted to nomenclature committee
CYP2K2 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP2K3 Onchorhynchus mykiss (rainbow trout)
no accession number
Buhler,D.R.
(5L7FL) 96.5% identical to 2K1
submitted to nomenclature committee
CYP2M1 Onchorhynchus mykiss (rainbow trout)
GenEMBL U16657
Yang,Y.H., Wang,J.L. and Buhler,D.R.
cDNA cloning and characterization of a novel cytochrome P450 from rainbow
trout.
Abstracts of the VII International Congress of Toxicology,
Vol. 7, No. 1, 10-P-2 (1995)
Note: 42% identical to CYP2K1
CYP2N1 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP2N2 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP2P1 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP2P2 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP2P3 Fundulus heteroclitus (killifish)
John Stegeman
submitted to nomenclature committee
CYP3A27 Oncorhynchus mykiss (rainbow trout)
no accession number
Don R. Buhler
submitted to Nomenclature Committee
56% identical to 3A12
CYP4T1 Oncorhynchus mykiss (rainbow trout)
no accession number
Patrick Falckh
submitted to nomenclature committee
closest resemblance to CYP4B
CYP11A1 Oncorhynchus mykiss (rainbow trout)
GenEMBL S57305 (1789bp) Swiss Q07217 (514 amino acids)
PIR S32197 (514 amino acids)
Takahashi,M., Tanaka,M., Sakai,N., Adachi,S., Miller,W.L.
and Nagahama,Y.
Rainbow trout ovarian cholesterol side-chain cleavage
cytochrome P450 (P450scc). cDNA cloning and mRNA expression
during oogenesis.
FEBS Lett. 319, 45-48 (1993)
CYP11A1 Dasyatis americana (southern stingray)
GenEMBL U63299(4619bp)
Nunez,B.S. and Trant,J.M.
Isolation of the cDNA encoding the interrenal form of cholesterol
side chain cleavage cytochrome P450 of the southern stingray
(Dasyatis americana).
unpublished (1996)
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL S50356
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17 alpha-hydroxylase/17-20
lyase) cDNA cloning, enzymatic properties and temporal pattern of
ovarian P-450c17 mRNA expression during oogenesis.
FEBS Lett. 301, 60-64 (1992)
Note: have not been able to download from NCBI
CYP17 Oncorhynchus mykiss (rainbow trout)
GenEMBL X65800 (2287bp) Swiss P30437 (514 amino acids)
Sakai,N., Tanaka,M., Adachi,S., Miller,W.L. and Nagahama,Y.
Rainbow trout cytochrome P-450c17 (17
alpha-hydroxylase/17,20-lyase). cDNA cloning, enzymatic properties
and temporal pattern of ovarian P-450c17 mRNA expression during
oogenesis
FEBS Lett. 301, 60-64 (1992)
CYP17 Oryzias latipes (medaka)
GenEMBL D87121(2421bp)
Kobayashi,D., Matsuyama,M., Tanaka,M., Fukada,S. and Nagahama,Y.
Structural analysis of medaka P-450c17 and expression in the
ovarian follicle.
unpublished (1996)
CYP17 Oryzias latipes (medaka)
GenEMBL D87122(2302bp)
Kobayashi,D., Tanaka,M., Fukada,S. and Nagahama,Y.
Presence of a Novel Cytochrome P-450c17 Transcripts in Medaka
Gonads
unpublished (1996)
CYP17 Squalus acanthias (dogfish)
GenEMBL S77384 (1964bp)
Trant,J.
Isolation and characterization of the cDNA encoding the spiny dogfish shark
(Squalus acanthias) form of cytochrome P450c17
J. Exp. Zool. 272, 25-33 (1995)
CYP19 Ictalurus punctatus (channel catfish)
GenEMBL S75715(2102bp)
Trant,J.
Isolation and characterization of the cDNA encoding the channel
catfish (Ictalurus punctatus) form of cytochrome P450arom
Gen. Comp. Endocrinol. 95 (2), 155-168 (1994)
CYP19 Onchorynchus mykiss (rainbow trout)
Tanaka, M., Telecky, T.M., Fukada, S., Adachi,S., Chen, S. and Nagahama, Y.
Cloning and sequence analysis of the cDN A encoding P-450 aromatase
(P450arom) from a rainbow trout (Onchorynchus mykiss) ovary, relationship
between the amount of P450arom mRNA and the production of oestradiol-17-beta
in the ovary.
J. Mol. Endocrin. 8, 53-61 (1992)
CYP19 Carassius auratus (goldfish)
GenEMBL U18974(2939bp)
Gelinas,D.M., Pitoc,G.A. and Callard,G.V.
Isolation of goldfish brain aromatase cDNA and analysis of
expression during the reproductive cycle and after steroid
treatment in vivo.
unpublished (1996)
CYP19 Oryzias latipes (medaka)
GenEMBL D82968(1851bp)
Tanaka,M., Fukada,S., Matsuyama,M. and Nagahama,Y.
Structure and promoter analysis of the cytochrome P-450 aromatase
gene of the teleost fish, medaka (Oryzias latipes)
J. Biochem. 117 (4), 719-725 (1995)
CYP19 Tilapia nilotica (Cichlid fish)
GenEMBL U72071(1804bp)
Chang,X.T., Kobayashi,T., Nakamura,M., Kajura,H. and Nagahama,Y.
Isolation and characterization of cDNA encoding the tilapia
(Oreochromis niloticus) cytochrome P450 aromatase (P450arom):
Changes in P450arom mRNA, protein and enzyme activity in ovarian
follicles during oogenesis.
J. Mol. Endocrinol. (1996) In press
CYP26 zebra fish
GenEMBL U68234
White, J.A., Guo, Y.-D., Baetz, K., Beckett-Jones, B., Bonasoro, J.
Hsu, K.E., Dilworth, F.E., Jones, G. and Petkovich, M.
Identification of the retinoic acid-inducible all trans retinoic acid 4-hydroxylase.
J. Biol. Chem. 271, 29922-29927 (1996)
Note: new family in vertebrates, homolog to human ESTs R51129 and R21282
Unidentified fragment
Oncorhynchus mykiss (trout)
GenEMBL T23107 (310bp)
Gong,Z.
unpublished (1994)
Note: 45.1% identical to 52A10, with similarity to other 52 family members