A new Human P450 has been found and it has orthologs in mouse and bovine and
fish. It may be vertebrate specific since it is not found in fly or worm. The
new sequences are all being named CYP20 (a long reserved and unused animal p450
name)
Human CYP20 (phase of introns shown) AC011737.8|AC011737 Homo sapiens chromosome 2 clone RP11-33N4, WORKING MLDFAIFAVTFLLALVGAVLYLYP (0) ASRQAAGIPGITPTEEK (2) DGNLPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTS (1) DPFETMLKSLLRYQSGGGSVSENHMRKKLYENGVTDSLKSNFALLLK (0) LSEELLDKWLSYPETQHVPLSQHMLGFAMKSVTQMVMGSTFEDDQEVIRFQKNHGT (0) VWSEIGKGFLDGSLDKNMTRKKQYED (1) ALMQLESVLRNIIKERKGRNFSQHIFIDSLVQGNLNDQQ (0) ILEDSMIFSLASCIITAK (1) LCTWAICFLTTSEEVQKKLYEEINQVFGNGPVTPEKIEQLR (2) YCQHVLCETVRTAKLTPVSAQLQDIEGKIDRFIIPRE (0) TLVLYALGVVLQDPNTWPSPHK (2) genomic seq stops here the rest is cDNA FDPDRFDDELVMKTFSSLGFSGTQECPELR (2) intron site based on fish genomic DNA FAYMVTTVLLSVLVKRLHLLSVEGQVIETKYELVTSSREEAWITVSKRY AK020848 Mus musculus Cyp20 adult retina cDNA plus ESTs for C-term MLDFAIFAVTFLLALVGAVLYLYPASRQASGIPGLTPTEEKDGN LPDIVNSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTTDVLKQHFNPNKTSDPFET MLKSLLGYQSGGGSAGEDHVRRKLYGDAVTASLHSNFPLLLQLSEELLDKWLSYPETQ HIPLSQHMLGFALKFVTRMVLGSTFEDEQEVIRFQKIHG TVWSEIGKGFLDGSLDKNTTRKKQYQEALMQLESTLKKIIKERKGGNFRQHT FIDSLTQGKLNEQQILEDCVVFSLASCIITAR LCTWTIHFLTTTGEVQKK LCKEIDQVLGEGPITSEKIEQLSYCQQVLFETVRTAKLTPVSARLQDIEGKVGPFVIPKE 360 TLVLYALGVVLQDPSTWPLPHRFDPDRFADEPVMKVFSSLGFSGTWECPELXFAYMVTAV 540 LVSVLLEKLRLLAVDRQVVEMKYELVTSAREEAWITVSKRH* Bovine CYP20 MLDFAIFAVTFLLALVGAVLYLYPASRQAAGIPGITPTEEKDGNLPDIV NSGSLHEFLVNLHERYGPVVSFWFGRRLVVSLGTVDVLKQHINPNKTLDPFETMLKSLLR YQSDSGNVSENHMRKKLYENGVTNCLRINFALLIKLSEELLDKWLSYPESQHVPLCQHML GFAMKSVTQMVMGSTFEDEQEVIRFQKNHGTVWSEIGKGFLDGSLDKSTTRKKQYEDALM QLESILKKIKERKGRNFSQHIFIDSLVQGNLNDQQILEDTMIFSLAS CMITAKLCTWAVCFLTTYEEIQKKLYEEIDQVLGKGPITSEKIEELRYCRQVLCETVRTA KLTPVSARLQDIEGKIDKFIIPRETLVLYALGVVLQEXGTWSSPYKFDPERFDDESVMKT FSLLGFSGTRECPELRFAYMVTAVLLSVLLRRLHLLSVE GQVIETKYELVTSSKEEAWITVSKRY 498 Fish homologs CYP20 From oryzias latipes (fish) MLDFAIFAVTFVVILVGAVLYLYPSSRRASGVPGLFPTDEKDGNLQDIVDRGSLHEFLV GLHEQFGPVASFWFGRQPVVSLGSVDPLRQHINPNHTTDSFETMLKSLLGYQAGAGGGAN ESVMRKKLYESAINNALKNSFPAVLKVAEELVDKWSSVPEDQHIPLCAHLLGLALKTV Human to fill gap in fish based sequence TQMVMGSTFEDDQEVIRFQKNHGT (phase 0) VWSEIGKGFLDGSLDKNMTRKKQYED (phase 1) 227 this part from Takifugu rubipes pufferfish ALSEMESTLLSVVKERKSQRNKSVFVDSLIQSTLTERQ 265 IMEDCMVFMLAGCAITAN 283 (1) Tetraodon nigroviridis freshwater pufferfish 284 VCIWALHFLSSSEDVQDRLHQELEEVLGSGPVSLEKIPQL RYCQQVLNETVRTAKLTPVAAGLQEVEGKVDQHLIPKE TLVIYALGVILQDSHTWDAPCR FHPDRFEEESVRKSFRLLGFSGSQTCPELR VAYTVATVLLSAVVRQLRLHRLEDTLVEVRSELVSTPREETWITFSRRN