41 P450s from Neurospora crassa

>CYP65C1

41 P450s from Neurospora crassa

including three new ones CYP526B1, CYP559A1 and CYP560A1

D. Nelson Last modified March 18, 2004

This complete revision was prompted by submission of Neurospora P450s from Ralph Dean. Many of the sequences have undergone some changes and the result is a better and more complete P450 gene set.

Contig numbering:

There are 821 contigs (longer than 2kb) in 173 supercontigs

example: cont3.282 indicates version 3 of the assembly

contig 282.

31 supercontigs have P450s. 8 supercontigs have more than one P450. Only two contigs have more than one P450.

contig 332 has CYP527A1, 528A1 and 529A1. 527A1 and 528A1 are about 1700 bp apart. 529A1 is about 80,000 bp away.

contig 625 has CYP552A1 and 526B1. These two CYPs are about 3200 bp apart. Other P450s are farther apart, so there are only two P450 gene clusters and they have only two genes each. These genes are not closely related to each other. There may be more clusters after the genome is assembled and the supercontigs are ordered.

The 41 sequences occur in 39 families. CYP65B1 and CYP65C1 are about 45% identical. 527A1 and 527B1 are only 42% identical. This means that recent gene duplication has not played a major role in P450 diverisification in Neurospora, like it has in plants and animals with large gene clusters of very similar P450 genes.

contigs are ordered inside supercontigs, but supercontigs are not ordered. They are named by decreasing size.

Therefore, contig 3.101 and 3.102 are adjacent (in supercontig 5), but contig 3.102 and 3.103 are in two different supercontigs and they are not adjacent.

see the link below for a list of contigs and supercontigs

http://www.broad.mit.edu/annotation/fungi/neurospora/supercontig_table.html#c1

The sequences were blast searched against the WGS section of Genbank to assign accession numbers, nucleotide numbering and intron exon boundaries.

>CYP51 AABX01000052.1 cont3.136 NCU02624.1 (version3)

2384 MGILQVVAGPLSQQFSQLGTVSQIGVAIASFLFVAVVLNVLQQFLFKKPN

EPPLVFHWFPLIGSTITYGMDPPRFFKENREK (0) 2139

2066 YGDCFTFILLGKKTTVYVGPKGNDFILNGKIRDVNAEEIYTVLT

TPVFGKDVVYDCPNSKLMEQKK (0) 1869

1806 FMKIALTTEAFRQYVPIISDEVTSYLKR

TADFKGKSGIVDIPPKMAQITIFTASHALQGKEIRDKFDETLADLYHDLD

MGFSPINFMLHWAPLPWNNRRDYAQRTVAKIYMDTIKERRARGETGAQDI

MWHLMNSTYKGDVPVPDHEIAHMMIALLMAGQHSSSSTSSWIMLRLASRP

DIMEDLYNEQVKNLGADLPPLTYEDLAKLPLHAAIVKETLRLHAPIHSIM

RAVKTPMPVPGTKYVIPTDHVLLAAPGVSATDESYFPQPDLWEPHRWEKD

SPLAPTIVRNVPSEEDDEKIDYGYGLVSKGANSPYLPFGAGRHRCIGEQF

ANVQLQTILAIIVRNFKFRNVDGSDKVIGTDYASLFSRPLEPAKIYWERR

EGCQL* 655

>CYP53A4 AABX01000266.1 cont3.41 NCU01086.1 (version3)

243627 MAIISLLMSPWAPVVLLAGVAFYYLVPYFVTYSALRK

IPSPFPAQFTDLWLLSVCRRGNRYQRVDELHKKLGPVVRIQPNH

VSICDDAAIPTIYGHGNGFLN (2) 243932

243994 DFYDAFVSIRRGLFNTRDRAEHT

RKRKIVSHTFSAKSVQQFEPYMHSNLELFVKQWDSMIKNSKNPDKAAHLD

CLEWFNYLAFDVIGDLSFGQPFGMLSSGADMAEIRSSPDAAPIYAPAIEI

LNRRGEVSATLGIHPALKPFAKYLPDPFFTKGLAAVENLAGIAIACVKSR

LDNPPPVTRKDLLQRLMEGRDEKGEPLGREELTAEALTQLIAGSDTTSNS

SCALLFHAVRTPGVMQKLQAELDANIPPEVDVPTYDMVKELPYLEAVINE

VLRFHSTSGIGLPRQIPHDASQGVHIQGYYLPPGTVLSVPTYSIHHSKEI

WGPDADEFKPERWERLTARQKNAFIPFSHGPRSCVGRNVAEMEMKLIVAT

WARRYEVKLLQDYMDTREGFLRKPLGLKVGLKLRK*245220

>CYP54A1AABX01000809.1 cont3.282 cyan = differences

from an earlier seq. version

4170MALTQLAHSASALLPLVLPSIAAILAFAIFQRYFAPNPLSNLPIV

     GEEYHGYEKKRQAYLTKAKDLYLEGYTK (0)3952

3824FKHGLFRIVTPNRNSVIVVSPRFLDELKKLPDDVVSFDAANDD (0) 3696

3565SMHTKYTLIPTHEPILPHTIKTSLTPSLPRLNPQLSEEVQIAFSQ

EIAPLMSSSPSDWAPININSKLLRIVAKVSGRVFIGPELCHDERYLEAAV

GYTVSVMEAQRAVERMNPWVRPIAAWRLKEVRKLAQMERDATAFLRPVVE

ARREKQRKGEEKDNDMLQWLMDSADQGQGKQHGKWGEDTTTTRKLARLQL

AISFAAIHTTTLVTTHAVYSLAADPKLQATLREEIQSVLQEHKGVFNTSA

LQAMKKTDSFLKETMRFHPLGQTSFNRKVLRTFALSNGQVIPKGSTIEVP

NYAVSRDPEAYPNPDVFDPLRFYNLRNEAREKGEAEQAASGQFVSVNKEF

LTFGYGRHACPGRFFAANEIKMILANLVMTYEMGLVEGETERYRDWDIAA

GTIPDPTKDVMFRKL* 2333

>CYP55A6 AABX01000354.1 cont3.356 NCU06137.1(version3)

405 aa short seq. probably bacterial origin, solubleprotein

153215 MGALTENLEFPFRRASGLDPPPEYAWLRKTDPVTQVRLFDGSL

      AWLVTKYHDVCQVATDERLSKERTRPGFPELAAGGKEAAKNRA

      TFVDMDPPKHMQYRGMVQPIFEMEHVKELEPYIQKTVDDLLER

       MKNMGCEGGPVDLVQNFALPVPSY (0)152757

152692IIYTILGVPVEDLDFLTQQNSIRTNGSSTAREASAANQ (2) 152579

152519 ELLNYLRRLAEQRLQEPKDDLISKLMTEQVKAGKLDTADAVQM

       AFLLLVAGNATMVNMIAL (0) 152337

152273 GIVTLFLNPSQLEELKRDPSVVPAFVEELSRFHTASAMAIRRV

      AKEDLEIRGKTIKKGEGVIASNLSANRDEEVFKDADKFDMHRE

       WPVKELGFGWGEHRCIAEFLAKAELKAVF(1) 151893

151771 ETIFKKLPDLKIAVPFDEIEYTPLDRDVGIVKLPVTW* 151658

>CYP61 AABX01000229.1 cont3.300 NCU05278.1 (version3)

     MASNATSFTSPLASAKLGTVAVPPQLEYVIDTISQASGWTIVFT

      LLAVLVAWDQ (1) 24057

24127 IKYVLNKGTIAGPSWKIPFIGPFLESLDPKFEEYYAKWLSGPLS

      CVSVFHK (2) 24279

24350 FVVIASTRDMARKVFNSPSYVKPCVVDVAHKLLGADNWVFLDGPA

HVEFRKGLNGLFTRRALEIYLPGQEEVYNRYFKSFLEITKNAGGKPVPWM

PHFREVITAVSCRSFVGHYISDEAVKKIADDYYLITAALELVNFPIIIPY

TKTWYGKKAADMVLAEFAKCAAKSKVRMAAGGEATCIMDAWIKQMIDSKK

WREAVASGNTEGLEKPTHLLRDFTDYEISQTIFTFLFASQDATSSAATWL

FQIMAQRPDVLDRVREENLKVRNGDIHAPINMEQLESMTYTRAVVRELLR

YRPPVLMVPYLVKKAFPITEDYTIPKGSMVVPTTYMALRDPEVYPNPDYF

DPERYYSGDAEAKGAKNYLVFGVGPHYCLGQHYAQLNLALFVGKASLLLD

WKHHPTPLSEEIKVFATIFPK (0)25597

25660 DDCPLTFEERKW* 25698

>CYP65B1 AABX01000364.1 cont3.489

23181 MMMMMMNLSHLLPLGIPLPSPTSLIGIFLIG (0) 23273

23368 TSILVSSILLSAIYNLTLH

     PLARHPGPFFHRASILPYLYRQITGTLPCSILDFHARYGPVVRI

     SPNALSFADPQAWKDIYGHRPHGEEEFAKLNLFYRIKGSPPSLL

     SETKEAHGTLRKLMAHGFSDRSMRAQEGIIGGYVSALIRGLRGNC (1) 23823

23968 QEQDERVPLDMVSWYNWTT

     FDIIGDLAFGEPFGCLEKAEYDPWVDAVGKSVRFGCVMFALRLL

     GLEDWVCPLVRKLSGNARRFHRKRTMDKLQRRVKLTKERPDFLE

      GLLQKREEW (0) 24315

24423 GIDMDALAANASLLIVAGSETTATLLAGATYMLLRDPEAMKKLT

     EEVRSTFKSEEEITLSSVGNLEYMLACLNEAMRLYPPVPIGMPR

      VVPKGGAKVAGTFVPEG (0) 24737

24819 TVVAVWHWATSHNEQHFVEPFEFHPERWMQDPRFANDRLDAVQP

      FSVGPRNCIGRS (2) 24983

25076 LAIAEMRLILTKVVYNFDMKLANPEKDWLDQHIYTLWDKHPLP

      VYLTPIERNGV* 25240

>CYP65C1 AABX01000034.1 cont3.25

thisseq deleted part of the I-helix added back (required)

20288 MPVIDKLAGVTGLSGLHLLLGAVGVT (0) 20365

20487 ILLYIIGSAIYNVFFHPLASYPGPLLQRASPIPFSIRHALGVQP

     FHTQALHDKYGPVVRIAPNHLSFTDVRAWRDIFGHIAGSRHGQE

     ELAKFKSFVRPIDAQPIHIISAGREKHGQLRRALANSFSDASMR

      GQGPLIGKYIDLLLHKLHEQGQEGKVPLNATSWYNSVTFDVAGD

     LIFGMSFGALDQNGNHSWLQYILGSLSSLAPMAALSYAGLHWLV

     QLLWRAKGAEVFRKSMESVDLMLKERLKMPVDRNDLFEGLVQRQ

      EKL (0) 21287

21563 GLSFEELASNAWLLVL (2) 21610

21675AGADTTATTLSGTTYLLTQHPEVLKRVTQEVRSSFKSVEEIDIS

     SVNRLTYMLAVLNEAMRLYPPVTSLTARIVPRGGAHVLGEYLPEG (0) 21941

22014 TIVDIQQWSMNHSKENWVDPWAFNPARFLDDKETAREKGNVLDAL

      QTFSVGPRNCIGRN (2) 22190

22266 LAYAEMRWILARILFDFDLRGAPTNQNWIERQKAYGLWDRIPLD

      VYFEPARERK* 22430

>CYP68D1 AABX01000146.1 cont3.306 NCU05376.1(version3)

10526 MANPSATPSSIPSWMERLDIKSITDPSATPFSYLVTAFLLAVVV

     YSLQGPRFPKNIKHLNPKGPLEFSDTRPKKEFVYGSRQMLANWF

     KANPNKPCRVISDFGEAIVLPPRMANEIKNDDRLSFTRWTYK (0) 10915

10981 AFHGHLPGFEGFGEASRESHIVQEVIMRDLTKYL (1) 11082

11140 NKVTEPLAQETSMAMEANLPKAANGEWSTINLRSKI

LPIVARISSRVFLGEELCRNEEWLKVTQQYTIDGFGAAEDLRLWPAALRP

IVHWFLPSCQRARADVRVARSILDPVLKKRRQEKAANGGKAEHDDAIEWF

ERTAKGKYYDPAVAQLVLSLVAIHTTSDLTCQVMTNLMQNPEFIAPLREE

MIQVLSEGGWKKTSLYNMKLLDSVIKESQRVKPTGVASMRRYAEKDVTLS

DGTFIPKGGFVAVSAHDMWNSEVYEQAEKWDGRRFLRMRETPGAGKENVA

QLVSTAPEHLGFGHGQHACPGRFFAANEIKIALVHLLLNYEWRLPEGSDP

KIRTFGFSMGVDPSLKVEYKGRQPEIEL* 12234

>CYP505A2 AABX01000234.1 cont3.292 NCU05185.1(version3)

28427 MSSDETPQTIPIPGPPGLPLVGNSFDIDTEFPLGSMLNFADQY (1)28555

28609 GEIFRLNFPGRNTVFV (0) 28656

28709 TSQALVHELCDEKRFQKTVNSALHEIRHGIHDGLFTARNDE

PNWGIAHRILMPAFGPMAIQNMFPEMHEIASQLALKWARHGPNQSIKVTD

DFTRLTLDTIALCSMDYRFNSYYHDDMHPFIDAMASFLVESGNRSRRPAL

PAFMYSKVDRKFYDDIRVLRETAEGVLKSRKEHPSERKDLLTAMLDGVDP

KTGGKLSDDSIIDNLITFLIAGHETTSGLLSFAFVQLLKNPETYRKAQKE

VDDVCGKGPIKLEHMNKLHYIAAVLRETLRLCPTIPVIGVESKEDTVIGG

KYEVSKGQPFALLFAKSHVDPAVYGDTANDFDPERMLDENFERLNKEFPD

CWKPFGNGMRACIGRPFAWQEALLVMAVCLQNFNFMPEDPNYTLQYKQTL

TTKPKGFYMRAMLRDGMSALDLERRLKGELVAPKPTAQGPVSGQPKKSGE

GKPISIYYGSNTGTCETFAQRLASDAEAHGFTATIIDSLDAANQNLPKDR

PVVFITASYEGQPPDNAALFVGWLESLTGNELEGVQYAVFGCGHHDWAQT

FHRIPKLVDNTVSERGGDRICSLGLADAGKGEMFTEFEQWEDEVFWPAME

EKYEVSRKEDDNEALLQSGLTVNFSKPRSSTLRQDVQEAVVVDAKTITAP

GAPPKRHIEVQLSSDSGAYRSGDYLAVLPINPKETVNRVMRRFQLAWDTN

ITIEASRQTTILPTGVPMPVHDVLGAYVELSQPATKKNILALAEAADNAE

TKATLRQLAGPEYTEKITSRRVSILDLLEQFPSIPLPFSSFLSLLPPMRV

RQYSISSSPLWNPSHVTLTYSLLESPSLSNPDKKHVGVATSYLASLEAGD

KLNVSIRPSHKAFHLPVDADKTPLIMIAAGSGLAPFRGFVQERAAQIAAG

RSLAPAMLFYGCRHPEQDDLYRDEFDKWESIGAVSVRRAFSRCPESQETK

GCKYVGDRLWEDREEVTGLWDRGAKVYVCGSREVGESVKKVVVRIALERQ

KMIVEAREKGELDSLPEGIVEGLKLKGLTVEDVEVSEERALKWFEGIRNE

RYATDVFD* 31858

>CYP507A1 AABX01000414.1 cont3.575 NCU09185.1(version3)

98714 MALTELLGALPLVSSGMSKLQVAAIYLAISFVSWYIISSFTDWY

     RLKAFPGPPTTGFSNLWAARAIWTGKAHKIFPATQEKYGPITRI

      GPNALMVCDAATVVHINGVRGGYARSQDFYDCIRMDPWDHTVLS

     ESDSAMHNERKTKVYAGYHGKGEMDMEKDVDMVIAEAVELVRKK

     YMNSAATSGSKPPLDYTRIARYIAVDSVTQTGFGKAWGDVREEK

     DHFGWLGFADMIVGYLHSLAYVPAISKIVISTPLMVLLGPKPTD

      KSGIGAFLG (2) 97896

97821LIEREVARRYKDGSAKGTPLKADTGRSSML (0) 97732

97669 DEWVKNGISQRTAVLEVSAQLPAGSDTTASALQGTMLYLLSTPS

     AYIRLKSEISTAIRTGLISSSSIITDQEARKLPYLQAVLYEAIR

     MMPPLMNGFPRQVPAGGDTICGRFVPGGTDIFVNYVGMLRDKSV

     FGEDANVFRPERYLEGDEERRNRMWKTTDLAFSHGRWRCLGQRL

      AWVQLQKVVVE (0) 97109

97044 FMRNFEMQIVDPMHPCRLRCYCTPEMDDLMVKVTEAKWD* 96925

>CYP527A1 AABX01000356.1 cont3.332 NCU05848.1(version3)

4191 MLQELLSLAQGPYALPILAASLVAYLLVSITITYRRLSHFPGPFLASFSP

LFMLKVLFSGHHADGYSRLNQQYQSPLVRIGTTDLITDDPAIIRHMNGAR

SAWGRSNWYRAMTLDPRGGSLFDEPNTKTHDLFKARLSFGYSGRENPGLE

GDIDEVIGMLIQYIKSRYISDDERGVLKRMDLAKVTQFFTLDVITRIAYG

KEFGWLKTDKDQFEWTSTVIKAVPANSLIAELPLLQKIFLSKWFLQWFGP

KHSDANGMGRVMGMAREMVAKRFGEKAEDRKDMLGSFVRHGIEQQACEVE

VLFQILAGSDTTAVAIRSTFFHLATSKKAYVRLLEEIDRAVKEGEVSSPI

RAEEAKHLEYLQACVYEGLRMQAPFSGLCMKSPPKGGDFINGMFIPEGTR

VGHNFGGLIRRRDVFGEDADVFRPERWLNAEPAKRLEMQQTTEMVFGYGR

WACLGKPVALMELSKVFFE (0) 2785

2725 FFRRFDLQLVEPKNPWHEFNVNMCFQSDLWVKITERFPEGEKV* 2594

>CYP527B1 AABX01000074.1 cont3.486 NCU08112.1(version3)

26652 MASLGGFFDPSRLTTASPKAILITTFSLLIVYYITSSIIAWYRLRHFDGP

      WLGKFSYLWIFKIIHSGQMGEAAHAAQDKYGGVEPGYPSTVRIGPSDLIT

     TELDLIRRMSGARSRYTRSDWYKLNRLDPYDDNMFSTTSTTYHDKLKSKM

     APGYAGRDNTGVEKDIAHVISKLVDKIRTKYAAPKGSTSPAGREMLEFGQ

     MAQYFTLDVICKTAFQVEFDHITKEEDVFGYIYMVHNLVYGNAMGATIPI

      IGKVLSNPLFLKMFGPTTKDPRGMGLIMK (2)27488

27541 VAREIVAKRFAPDAKDQQDML (0) 27603

27668 GSFIRRGLTQRQCETESIFQILAGSDTTATGIRSGLLYLCSNPRAYTRLQ

     REIDERIASGLISSPCITNAESLAFPYLQAVIYESLRMRPPFDGLPFKIV

     PPEGDYTRDGKFIPGGTKITATFGAMQRNKEVFGVDSDIWRPERWLEEEG

     CDAERRREMKSVVEMVFGYGRWQCAGKMVAMLELNKIFCQ (0) 28237

28368 LMRNFDFQLANPTQPWKSRNYHIFFHDDFYLTVTDRHSKTEDEKSS*28518

>CYP528A1 AABX01000356.1 cont3.332 NCU05849.1 revised

appears to have a 14 aa insertion in the beginning ofexon 3

8219 MSLPTLQDVLATSISLLKTTTPLLKRITSLLLKTTTSLLFIIVVTILAIA

    VYRLFFHPLAGIPGPRLAALSNIWHAYHARNGRM YLLGKSLHKRYGPVVR

    VGPNELWFDSKEAFKTIY (1) 7866

7779 SAGSGFEKSDFY (1) 7744

7659 LSTLLTKPTLSLKFSPFKFP

    FPNLQLPLQIHHPDTLDLLSERHLPRYRLQRRLIGPLYQLSSLKKFEPQI

    DAVLDRAIAQLRTLQGQEVDLKEWMHIIAVECLGAVVLSWSPGYIRDKSD

    GGTSVQGYLGWKRKSVFGLFPGITILSFLEG (1) 7207

7173 GVARWWSNTWGVTFATPKGFKPFFTAVYQKVSRRVSMAALLWMSDE (0)7066

6953 MEEDKGKKDLLEDLIKLYQSKPEQFTETYLRRMAMTNFGAGHET

    LCSALTACMAMIGTHAAVQARVAEEVGSHLRGGGTVPFDTAAKQLPYTLA

    AIKEAQRLHPVIAMSLSRTVPRDLDGGGVMLHGHWVPEGTT

    VGCNPVALHRNQEIFGEDADVYNPSRWLP (1) 6490

6154 LERKRLREMLHLNLTYGGGARTCPGRHLAELIVWKVIPRLVAE

    FRVEVTHMPRDEEKNMERYFMSMLTGVKVRFLERREDSGDMA* 5897

>CYP529A1 AABX01000356.1 cont3.332 NCU05874.1(version3)

88106 METSNLTLLLLYLAIPSLLISAVQQILSWRKRTALVGSSGSKAKFPGPRQ

      FPIVGRIHDLPRFSLWLKFKEWADEFGPIYTTSVPDATFVIISDEKIAEE

      LLVKRGHIYSGRPQIRSLIGHKEGVVYSALMDRH(1) 87705

87637 DTWKSQRKWAHAAMAA

     AYKHHFYGHVESEMRRFLVVLLFDPDRFLDHTREYCGRVMSRLAWDDATQ

     GRLNGESADKTLHCMSVSGPITNTMTPLWSLPASINPWYKYEVKREAELR

      QWWLGLFRKAKERMRKGELPSDTWAYRYFEQMVQDTAPSMPATARAPGPD

     VKNAERSPSTAAQEEAYEKKDALDQPEKDETFASCMIGFLNLVGVVTISG

     PLKFFQMAMALHPEWQHKAQAEIDEVCGDRMPTMKDFDKLPILRACLKET

     VRWRSGVPLGVPHQAERDDEFRGVKIKKGTIVLACEW (2) 86729

86660 TLNRNPQKYPDPE

      TYNPARYLDPSYPTFQAPLTRYPNFREGSSMHSFGWGRRTCLGQNIVDDE

     MFVFGAALLWAFEVTPKTCPVTGKEVPIDTHKTNSHVILEPVDFQLGYK

     VREGKKELILEGGKEFFGKV* 86262

>CYP530A1 AABX01000046.1 cont3.150 NCU02852.1(version3)

43902 MAVITTYLPIVVGIALLIRLLMVGRRPKNYPPGPPTIPILGNLHL (0)43768

43698 MPTKDVHLQFEKWAREYGPVYSLILGTKVMVVLSSDRAVKDLLD

KKSHMYSHRQEMYVGQTLCSGDMRILMLGYTA

KWRMSRKLVHALLNVSSAKSYVPYQMLEN

KQMLYEMITKPDEFLYNIRRYSNALTTTMVYGWRTPTYNDEKMRQLFDGF

SEFAEINQTGVAALIDFFPILRYLPDFLLPARKKAKELHKKELGLYKGHW

LKAKEVTLKGTITHCFCEDAVPVQKKEGFSDDEAAYIAGTLLEAGSDTTS

STLYGFVQAMLLYPEVQRKAQEEIDRVVGPDRLPTMDDEPHLQYCRAIVK

ESLRWMPTTIMGAVPHAVTQDDYYNGYLIPAHAAVVNNAWAINHDPVRAP

EPRKFKPERHLDDKLSLADSAANPDPTKRDQFTFGAGRRICPGIHVAERS

LFLGVARMLWGFSIAPKIGVDGKEIIPDQEKLTQGFVCMPEEYPATITPR

DERRKGIMEEEWFAAERECLDPETKQWKQGMNWVPKLSSTKV* 42205

>CYP531A1 AABX01000205.1 cont3.366 NCU06327.1(version3)

138867 MDSHAGLDGVLQPQVLKLTLRTLPSPFISFVVIPILSILGWYI

       ISWATNPLKKYPGPFLA (1) 138688

138607 GFTNWWRLYQTRTGRYHEIVYDLHKKYGPIVRIGPNTLDLDYP

       ELIKTIYSTDGKWLK (0) 138434

138374 TEFYHNNSAIVNGKITYHIFSTTSPTEHAKMKRPVAKYYSTSY

      VQALEPHVDVVLNDFCKYVEERFINVPGGPKELDFGEWLGY (1) 138123

138042HAWDLISSVTFSRRFGYMEHGSDFDGTIEKAEAALRYFQTVGQ

      IPILDYFLDKNPIKRVGPPNLVHPARIAVQSFVARLQGKDENY

      DPKNPDYLQHFIDSKESHPDLVDDNQIISDVLVNLLAGADTTA

      IALRAVFYYMLKNRSVYNRVSEEIQAAGFDRSKPVPYSGARQL

      PYLEACVREALRIHPAAAMLLERYVPADGITLPDGSFVPAGTA

      VGLNPWVVSRNKSIFGEDSDTFRPERWLQQPGEDDEAFHVRMQ

      KWNAADLTFGNGSRICTGRNFAMFELYKVCATLLHRFEIELAD

       PTKEWKVWGSWFTIQKDVIAKMKVRG*137062

>CYP532A1 AABX01000767.1 cont3.215 NCU04089.1(version3)

33962 MITDLIIKVLAQHWPAILCLVLVGWLVRNRYHNGLNKYPGPFLA

      SLTDWWRFIDVYKRRPEVTHIALHKKHGPIVRLGPNTLSFSDPE

      AIKTIYGLNKGFVK (0) 33657

33584 SDFYVVQQSTVKGHNLPSLFSTTDNEFHMQFRRCVNSAFAMSAL

      VQYEPFVDNTTKLFLEQTEKLYVEGGEKACDFTRWLQFYAFDVI

      GEITYSKRHGFIEHNKDIDGIVSYLTKLFNYVAP(0) 33219

33153 IGQIPLLDRLFLKNPLYLKLSQWGLIDGTMPVAAFARARMAERL

     PELQTEKTAILPTSEKQAQLKIQTPDLLSKFLAARSARPNFMTD

     TLVQTMAVSMAFAGSETTAISLSAVFYYLLRTPRALARLREEID

      EAARQGKFSDYETGLVTWAEAQTLPYLDACVKEAFRLHPAPGLP

     MERVVPPQGMEIVPGQFVKGGTIVGVSAWVLHRDESIFGERVEE

     FRPERWFVDETVAKEDMEGKKREEEEKRIKTMNGHMLQFGMGSR

      TCIGKNISLLEIYKLVPTLLRRFE (0) 32290

32200 IEFDDPSREWEIVNAWFVKQNNFITRFKVRDIVMPENKEY(2) 32081

32012KFDGRGVN* 31986

>CYP533A1 AABX01000277.1 cont3.42 NCU01134.1 (version3)

41700 MLKFFMGDFDIGPLSNMVTSNSSATRASSAPANGFQFIQQSVDT

     VAGTAIGLFIAYSAYKNLSEAYRRGDPNRKAPLPPSPPGDFLLG

      HYRHVPENESFRKYLEWSKKY (1) 42026

42089 NSDVLYFETFGTKWVVLNSYEAAHELLEKRGRKYSDRPRFVMFEE (2) 42223

42284 MGWAPTLTWLRWGPQWGLHRKVLAPPFAKTGCVSFQPLQRKQAM

     IMCKNLIENPEEWMSAVTHFAVAIMFKIGYGIDVDTPTDEWVKL

      AAEASEAVGKAGAPASSIMDRIPWSKFLFLATI(0) 42646

42717 RLKYAHENKKVIQNITERPFNASMSDY (0) 42797

42850 HERDLDPTRKLKECFVHRMYHLRGEDARRNRQNVYYEEDIKGGA

     ATMLIAGNDTTASTLNLIILYLTKHPRVQRRAQAEVDYILLTTG

     VPTEPVEDYRPSTPAPWASSSSNRAGIIRLPTLEDIPKFKYVNL

     ILQEVYRINPLSPLGIPHASVKDSSDIDPDGDDTYNGMRIPCGT

     IVYPNVWAMNRDEKRYREPERFFPERYLPKEEGGWGEPLPVGNF

     GFGRRVCVGQYLAENSLLIAIAMMLATIEFDLPIGPDGNLKDFE

     PQFSHKGQS (2) 43668

43748 IVLPFKVSIKPRSPMVEELLDRHILVAKMAEKAAEDGKKGGPTPSA*43888

>CYP534A1 AABX01000272.1 cont3.221 NCU04234.1(version3)

10018 MRLTPLVTLLGVFLAWATYKIYAGFRSNIAKARKTGYPVLIT (1) 9893

 9782PIYPLSPLWLTTRHFLLPILTRLLPERLYASYVFVMTPDWEY

      HPSPRSHFTRLGSDTF

VVASWNGLACYTSDAAVISQVMSRREAFPKDTRQYGILEIFGSNVVTTEG

QVWRMHRKVTSASFNERNAGHTFREAVRQTRGMVGGWFSSSSSSSSSSSS

SGSQEEGTGRREGDVEDMTGIITTLEHDTMRWALNIIGYVGFGLKLLWPH

ESMPKDMDPKLAKYGSLQPPYGYSMTFADSLATVLERIVILLLIPSAVLK

RIPERWGSWGSKLHEAWEAKVNYSKYMNAFLAEKVQDIRAGVQEREGMNI

MGQLVRSKYDPSSSEKNDGATMKLEDSEIMGNAFIMTLAGHETTANVIHF

AIVELAMHPEVQRRVQRDIDAIFGRDSDPETWDYEKSINALLASYIGAVI

NETLRLIPPVVVTPKRVADTDQWVQDGGQRHPMPAMMPVMPVISAVQRSR

RYWPALHDKTTKKGDTDDLDEWHPDRWYLPSMSQGQPSSDDEKGEGKQQQ

EENYGGYQGTDTSPTLFRPVRGSFIPFSDGARSCLGRRIAMVEMVAAIAV

LFQKYSVELAVDASDEEVQMMVPEQRRHVYAKAQEKAKETVQQAQSVVTL

KLTGGRHVPVRFVRRGKERFMGNEASC*7873

>CYP535A1 AABX01000066.1 cont3.39 NCU00987.1(version3)

1314 MGAATLIYEARWLLASVVLAIWAVLKTKQYYRLRHFKGPFGTGWFEIWHG

    LAYLSGHPHLKFKEATDKY (1) 1520

1580 GPIARIGPNELMTTSLELLAHINGARNRYTR

    TRWFYIAARFQPNSDNILSELDDNMHTKRRAQMAGG (0) 1780

1848 YSGKENRELESSVD

IHVAELVQLIRTKYRSTEIKTNPFDLAQKAQYLALDVISHIGFGEPFGML

LSDQDVNGYIKHADQGMNAITYLWGLGLTELVLDTPLMHLLGPSDTAKTG

FGAMTANTRKIISKRLEDDPGMTKGSDMLASWFRHGLDKEQLVTESILQL

LAGAETAATSMRCIMLYLVTNPRVYAKLQAEVDAAVKDGRAPPYPSVVSN

ATQRQLPYLWACCKEGMRMHPPVTNSSQKKVPPEGDAVTVEGKTVYLPGG

TNIGYCVWGLNRDQKIFGGDADCYRPERWLNEEDPKKLAQMNLVHEMLFS

    YGKFQCLGKPIALMEIGKAIFG (0) 2855

2934 LMRNFDWSVTNAAEPWKEMNAGALFLTHDFLVEATERSVS* 3056

>CYP536A1 AABX01000239.1 cont3.377 NCU06526.1(version3)

11364 MGVVLKVKDGKVSPTRVLVGATATVLASYWLYILLFAIVIRAASKRYASP

     LRKYPGPFLASCSRLWKVLSTASGRTHLDHIELHRKYGPVVRIAPNEVSV

     ASPEAARTLLSAGKRFFKTPFYSVFPPAENPDIFTEIREDAHAQKKRVAN

      VPYSMAAMQQLSPFIDDTIELLVRKIDEHIAQHKGVFDLGDYLHYFAFDV

     LGEVAFSRSFGFLAQGRDVDNAIKTIDKSQTYNGIVGQVPELDHLLRRNP

     LWRSIPWLSTKNALITRMALEEMGRRQPFNKDTAVLQTGDTRQDLMASLI

      LGHLKSPEKFGVGDVFAVAHGAI (2) 10399

10314 FAGSDSTASTMQSFFYHVLSSPSTYQS

      ILYEIQSAVATGVIPATGNLTWNEAQTLPYLQACLKEAMRLRPA

     VGLNITRFVPPEGAELDGHFFPGGTSIAVNGWVLHRDKLTFGND

      ADEWRPERWLENEEEARRMERYMFQ (0) 9895

 9825FGGGSHLCIGRNLALLEINKVVPRLLRDYRFELAHPGRELKATA

      SFFVVQSGLEVFVRRA* 9643

>CYP537A1Nc AABX01000723.1 AABX01000717.1 revised

57% toCYP537A4

521086MTLLLLLPIIIFLLHNLIYKPLTSPLSSLPGPLYTKFTSLVLK

      YHELRANRTRWIHALHVRYGPVVRIAPNEVSFASREGVKEIYC

      SGGSGFDKSGWYDLFKIYGRR (2) 521406

521667TMFTFLKKDD (0) 521696

521762HAKRKRILADRYANSNVMRQAPLSGIIERADR

      VVKRCAESLDGSLDLY (0)

       AVHSYAYDCSIRTTCFHPYGTDXLRN

       QQDEEIMREITFDDSLQ (1)

    97NRQLSYYSPTLHKAISKILYICGIK

      PRETPLADKFILDTAAKTDAAPFTLLDRLH (1)

       NSKIAQ

      IDQLDIAAELLDHMVAGIDTTGDALCFLMWELSQPRSRQIQTKL

      REELLSHPSSDINFDKLPYLDAVVMEGLRCFPAIPMSLPRIVPR

      GGKTIDGYFLPEGTTASCQAYSMHRIDMEAWGPDPDAFRPERWF

       EEKGDAARKKLFFAFANGARGCIGKH (2) 801

   907 LALAEMKILLREVYSKFTTVPDKTMTEE

      DMEMEDQLISTRPAGLKCLLKFEPIGGQ* 1077

>AACU01001722Magnaporthe grisea 58% TO 537A1

MLLFSPSITAIILGVCAAAYLIRCLRSPLRKVPGPWYSLFTGAG

LKYNELQGQRTRYIHKLHLKYGPTVRLSPSEASFTGPDAVREIYGSGGSGYEKTEFYDLFTVYGRR

TMFSTLDRES

HARRKRVLADRYANTNVMKQPALSGIAERARRFAELCQGSVGGSLDIYV (1)

NRLVKYYSPSLYHIVEPLLSFITQ

PRATPLADKFVLDTSAQTHASPFTLLDRLH

QRKNGGD LEQIDIAAECLDHMVAGIDTTGDALCFLMYQLSLPSSATFQRKLQAELAANP

DHDETPFDKLPYLDAIVQEGLRCFPAIPMSLPRYVPSGGRHVDGYFMPEGTIVSCQAY

SVNRINGDIFPHPETFNPDRWLEVEGDATRRRCMFAFSHGGRGCVGKHLALAEMKLLL

RDTYSRYSTIPDSKMTDDAMAMSDQIISSRPAGQKCLMRFIPLVDNAT

>AACM01000224 Gibberellazeae 56% TO 537A1

MFILSPSLISGAAALFLVAYIITRLSSATAKIPGPFVSNFTSLVLKWQELNANRTVYIHELHKRYGPVVRVAPN

EVSFTSIAAVKEIYGSGGSGYDKTEFYNLFQVYGKR

TMFSTLVKGD

HAKRRRMIGDRYANSNVMKAAPLAGIKERSSKFLQHCVESPERTADVFV (1)

NRLVQYYSPVLHNLGSKVLASFAK

PRSIPLADNFVIENAQQNGAASFTVLNRLKEXXXXXXXXXXXXXXXXX

KDSVLDTMDMAAECLDHMAAGIDTTGDVLCFLMWELSQPRSIKYQRLLREEFLRKTDMPFDELPMLDA

VLNEGLRCFPAIPMSLPRYVPQGGRVIDGFTLAEKTVVSCQAMSVHRINDDVFPEPDK

FHPERWLAAEGDADRRRHQWAFSSGGRGCVGKHLAMVEMKTLLRDVYSRYETEPDESM

KSESMVMDDQLISSRPLGKRCLLKFVPVKD

>CYP538A1 AABX01000024.1 cont3.89 NCU02031.1(version3)

27319 MLTPTTLLLLSPLLYLLTLLFRTALYRLRARSLGCSPVAVYPHK

     DPILGLDLFRESLRAMNTHALLPLWDARFKRYGNTHYTLTLGKW

     VLMTNEPENVKVILGTKMAEWPIDGPRLHASVAVLGKKSIFTTN

      GAQW (2) 26912

26807 REARGMIRPSFVRDQVADLHCFAKHVGNFLNAIPKDGSTFDMQE

     LLLSMTMDSSTDFLLGCSTNSLLQPSPEAKQFLEDFEYTSREAA

      KKSRLGTLLNWLPNGEFQATVTRVREYVRAYIRKSQAEKTDKKE

     RDYVFLHEILKSGADEEHAIDQVLSVIIAGRDTTAAAMTACFYY

     LARNPEAVKKLRKEIFDMEDEMPTWEQLKNMKYLNMIIKEG (1) 26156

26088 LRLFPPASTNSRAPIKDTVLPRGGGPDGKQPILVPKGQVVRWSL

      YSLHRRKDIWGEDAHEFRPERWDENLRVG (2)25870

25787 WEYIPFSGGPRICLGQQFALTQIEYALFKFFRAFKSIEPRDDNG

      PLLLRTNLTVTFAKGCLVSATPDSN* 25578

>CYP539A1 AABX01000502.1 cont3.571 NCU09115.1(version3)

(gcboundary at exon 3)

3508 MGLVEDVAGNLSLKNTPIIFGGVLLGLIVFRYLQVVWQNLRIARM

    GLRPPKIRDSGLF (1) 3681

3740 GIKFIKTNVKMAAEHKNLQFWQQMFRSIGGYTGEVRLVGHRIIFT

    SEPENMKAILATQFEDYGKGEGFHQEWKDFLGDSIFTTDGDLWHA

    SRQLIRPQFIKNRVSDLQCFENHMQMLFRTIANGGALNGEDQMVD

    MEAGNGKPVDISDLFFRYTLDAATDFLLGKDIKSLS (2) 4252

4308 TPVQPFADAFQEVQRVQIVIARAGPLNRFVPKKTFWEGLKVIDET

    INFYIDRALRLDEEELASKSKGDEGYTFLHALAGFTKNRQVLHDQ

    LMAVLLAGRDTTACTLSWAIYELARHPEAVAKLRAEILSVVGPDR

    APTYDDLKSMKYLQNVMNETLRLYPVVPFN (2) 4782

4867 VRMALKDTTLPRGGGPDGSQPIVILKDTPVGYSPLAMQRRPDLYP

    PVSEKFPDVEMFSPDRWFHWQPKPWQYIPFNGGPRICIGQQFALT

    EMGYVLTRLFQRYDRVVSYMDEIDGGKPRMKTDIVLMPGDGVKVA

     FFEAKRE* 5295

>CYP540A1 AABX01000009.1 cont3.484 NCU08062.1(version3)

46350MSSQTFFLVGDGPSTARGIVVDPKWKLEELKRAVGLAHHIAVPTG(1) 46481

46537GITFHFDADDEQELKTVEDVLRASGPVACRIDGNPVRDPQGPEGLPIVGS

YYEIFPDHLGNHYRLFRKYGHVIKTTNMGRTNYLTDDPAVALVALAESAYFTKKIT

EEHPLFGIKDNSAIFIGDTETENWRLAHKFLPPAMGPKAVRHYTPLMQEC

VRGSFKIFDELDSRGESWNVYQYMVKLASQTVGKFAMGIDYHHFDAVDTP

VHSLVTNIVKLLALNKKVTARGSWYQQLPFGDPARLREIRQITYGQLAET

IEAAPKSGIENLPLNEAATKASCVADYLLNAVDEKGEHFPKGLILSNMLV

VTGAGYTTTSALLSWCIYCIVTYPKMQDRLLQELIDHGINSETDWDPDLA

HSLPFLDAFLKETQRLHNASFQPGRTTKTDVVLPGGFRLPENVIMIPALY

AIHTNPEHWHDPFRFDPDRWDTEEVKSRHRGAYMPFATGPRSCIGFNFAL

LEIKVLLSELVYRYKFAREGFEAIEYDPEFQLIRPLNLYVTAKRRTEWPAKSSA* 48069

>CYP541A1 AABX01000779.1 cont3.283 NCU05006.1(version3)

7113 MSTPIPRPPGIPVLGNVFDITPSNTWWSLKALAEKY (1) 7220

7273 GEIFQVKILGKTIV

FVASAALAEEICDEQRFQKYVGGPIVEIRATVHDSLFTAFHHEQSWGIHH

RIIAPLLTPQAVSGYTDDINLCATEVIQKWASLGDSNVLEPLVDLNLLNL

EATSLTLFSQKLNCIQAGGHPVIQAMEDAVSEAIMRPTRPGLVNWLLYSS

KFKKATKTLRTWAADTVKYRQENPTDKHDMLWAFMNAKDPETGKGLSESE

ILDEIVTMPIGSATAPCAVTATIYYLLQNPEVVTKAREELDRVIGTGPLK

DEHLSQLHYIEGITRETLRLSCAAPGFNIEPIPRQNKADKSPLLLQGGKY

QVAHDQKLIIVLAGVNRDPAVFEDSLAFKPERMMGEEFDRLPKGVKKWYG

NGKRECIGKEWANNFLKIVTARLIHEIDFEVADEGYEFRQDGWFQIRPVA

FKVRVKPRVRA* 8550

>CYP542A1 AABX01000017.1 cont3.568 NCU09103.1 EST =AA902060

114647 MASTTAFLPRLTTITFSFLTQPLLIMFLLSLLTLYTIYI

      AYHRLYLSPLADIPGPRLAALTQWYEFYYEVILHGQYTF

      KIIELHKQYGPIIRINPWEVHIADPDFHRELLPTNTNRR

       RHRTPFFTKQFGAD (1) 114255

114135 ESIVATNDHDLHKLRRSAVGPFFSTQNTRALQPVIEERV

       DALLARLREHGKTKKDIPLNMMYAYSATSY (1) 113929

113861 DIISEYCFAKSEHA (1) 113820

113745 VEDPDFRAEITNGILTGSNYGKIFQHFPFLVPFLASIP

      PGMLAAISPFYRTFLHLRACITAQIGEIEKSLRSEEGK

      NAHLDIPHPTIFHSFVNTEALPPIEKSVPRIAQEGQV

       LVQAGTVTTSWALTISTFHLLDQHATALAKLRK

       *LREALPDDADEPVELAR

      LEQLPYLRAVIKESMRLSVGASGRITRVAPDETLRFK

      PSKLCLRCYPDDQLKTTAEDKEKEWLLPPGTEVSMTSY

      QITTNPEIFPDPHAFVPERWLGKENEMRLDKYMT

      VFGHGARVCLGMQLAYAEMYLMLSKMWRVWEGGPQVGG

       GEDEDGKEDVEEKKGRRDGRTTVGRLRLAEGVTVR

      DAEMAEDWFIPVPYRGSKGVRVYFESY* 112624

>CYP543A1NcAABX01000408.1 Neurospora crassa revised

27399MPCAFAITLLTVLVVLLVVQRWLERKRLRGGSRPLPGPT (1) 27515

27652NLPFIGRVHDIPEKGSWLKFHEWSKVYGPIYQTKMFGVTHVWIS

     SEKIAHDLLSRRANIYSDRPQIPNLPDNRTSGDYLALLGRN (1) 27906

27974DTWRRQRKLCNHLMHTSALASLHDYPTLERNRFLYLLSQSP

     ENYIEYIEQFTSRTIARLSWGSAHPAQILRHTTFGLLETIS

      PSGALPNVISFLRHLPLALSPWQKKEKARHDLEDKQFRSNI

     GFVKRMMDVGRAEPSFIRTYLEEQQGSINSTSTNKKCDEQK

     GKEAAADEAMHVVGLMAIAGALTIGSPIQSYILAMCHYPEW

     QARLQEEIDTELGGRCPMWEDREKLPLLRAVVKEVIRWRPPVPT (1) 28720

28825GIPHAVEKDDVYNGYFIPAGATIHALEW (2) 28908

29018AITRDEQTYPQAETFNPSRFLVPSYPTYREPLTIYPNLSGF

     SQFGFGRRTCQGVPIVEQDLFLTMGGMAWAFTISKKRDPVTGVEMPVHWNDYT

      PLLIAKPCRFPFDAVPRDEEKRKKMREMYEE

>CYP544A1 AABX01000494.1 cont3.721 NCU10011.1(version3)

(GCBOUNDARY at exon 2)

13303 MGIIHEKAHYVVAVALVAFLIRAFVLSQWNTIKRNGERLK (2) 13184

13120 KPPNTLPLVGNGLQFLQSRWKLFSWFDACQRKFGYETVAISVPT

     LPPGVLIHDPRNLDYVFKNEGIFTKGNFVKGRTWDLFGNGIINA

     EGDFWKTQRKAGLSFLNTANLRVLTDVALPQYLSESISQLRSST

      NGTVVDLQHVFHEITTKLMGKMAYN (0) 12650

12590 MAMHADDEFTVSFDYASGG

TAERFQNPLWFVTEIFLGAELRKSIAVVKNFGRHIVTKAVQDRQEKEFGE

EEGKLDQISGSLIQSLLDAIQDEQMVADAALTYLSAGRDTTGQALTWTFY

LLMRHPRVVAKIREEATQLLKEKNVTLTPDQFDSSLFNPVTMPYSMAVFY

EVLRLYPPIPFEIRQCNEDVTLPDGTFLPKSSILVWCLWAMQRSKLTWGD

DADEFRPERFLDGNKLISRSPSEFPVFYGGPRTCLGRKMAEAIAAQVIPT

MACLFDFVPTSDEERTSKTSLTLPMEGGLPVTVKTLTGEEREKLVRIPEI

RFSEH* 11616

>CYP545A1 AABX01000329.1 cont3.414 NCU07092.1(version3)

9756MQLLTLVIVGLFMLIVAVVHFIKAFREVNDPNGI

PGPTQIPYLGRVHDLPIQFMWLKFKEWADKYGQQGFYRTMMLGAEFIVVT

DEKVAEDLLVKRAKYNSDRPVIQSLFDSKSTHGSMEYLPLM (1) 9382

9297AYWARQRKLS

HSYLTEATKAHYYGVMYFEVQRWMARLLENPEDFQHSIEDMSSKVMCQLTWDDP

SLSEYCTKSAWGLLTQMSPAGPITNVLTPLWHLPTLINPWKRAERKRHDE

QQAWWMERLLTCREKLARGELRPCWTRQFLEKTSQKTSISGDYEASCVIG

MLALVGIFTVVGPMSYWLVSMVHNPKWQEAVQREVDEVCGNRMPRLEDAP

RLPILRACIKETMRWKPNVPTGVAHETEADDHYQGYFIPKGTRILPFDW (2)8509

8448 SFLRNPVKYPDPENFRPERWLEPGWPTYKEPLTQYPTIKGLTSFGWGQRQC

LGMSLTQDELIVGCGALAWLFNLRHKRDPITGRELPVPLDRSNSLLIIKP

DPFQMEFEPRSKERKKEALRVWKESEAKDRARRERWLRNVKEGKPNVIKE

PKVLQPTVKIPSPSPAAAVPAALVDGGEVRDELSKTVQVVKEKSGGVNGH

GDSLAEKAVMDVKKKADISITIARLDSTACVY* 7747

>CYP546A1 TWO PIECES AABX01000340.1 cont3.411

16866MLSDKLLDLFALHKTQEALADVNLYKLLVLALALVLTAIIIDYG

     YMLWLRSKMPTGPFPWPIIGNTFSLPENKPWIYFEELSKKYEKP

      LITFWIGR 17153 (2)

17210NPTIWINDAWCAHEILEKKAQIYASRPRMVVFGELGTGQKNLVT

      MRILNNNDRDRWRIHRKLMHLGVGIQSVRGYREIQNNESKVVAL

     DYLREPKEYVKHLERYATSVVSVIAFGRRVASYDDPIITEVIAL

      MQLAADLNVPGKRFPMLLETFPXXXXXXXXXXXX17671

      runs off end

AABX01000338.1 cont3.412

 145 RGGGQKGGGGGHYFFHSLALEALAQYESKSPLAKASMPTPYIKTLM

 283 DASRQYSLPEAELSSLTGNLFGAGSDTSSSTLITFMLSCLAFPDA

    MHKAQAELDRVLGGPAGGRSPHWDDAPNLPYINAYVKEVLRWRSV

    AIIGGQPHSNTSPDYYKGYFIPPHSWVQGNVWAIHHHEREFPDPD

    RFYPDRYLPGNDHHRPFPGEKGYMTFGWGRRVCSGQALAEQGTWI

    SVARLLWGFTIRKYRDPQTGKEEEVDIFAYTNGLNMRPQPFRCEI

    VPRSEEIREIIVREGEQALRDLKVLDGENRYRMSTFYQQKKREVA

     EMPEFDEKGNIRMVKVK* 1146

>compareto AACU01000188.1 Magnaporthe grisea

MIFDRLTSQASLAKLEDVISNVPSSKLITLSVLIIAVTALIIDY

GYMLYLRSRMPPGPFPLPIIGNTFSLPDNKPWVWFDELSKRYNAPLVTIWIGRNPTVW

ICDAWCAHDMLEKKAQLYSSRPRMVVFGELGTGQANLVTMRTRNQAERDHWRVHRRLM

HLGVGTQVVRRYRGVQDAESRVVALDMLRDPAAFVSHLERYATSVVSILAFGRRVASS

SDPIITEVIAVMQLAAELNVPGKKFPMLMETFPILAKFPRSMPWMKGLGARGRKGGHY

FWHSLAQEAVEQLDSRTDEERRNIPKPYVHTLFAENDKYKISTEEISNLTGNLFGAGS

DTSASTLVTFVLACCAFPEPAHKAQRELDRVVGHGRSPSFGDQADLPYVNAFVKELLR

WRPVAIIGGQPHSPTADDDYKGWRIPANSWVQGNLWAIHRNPRDFPDPDRFEPDRFVE

GSEWQRPFPGDRGYMPFGWGRRVCSGQALAEQGVWITVARLLWAFDMRKKRDPVTGAE

VPVDIFAFTNGLNMRPQPFLCDITPRSEEIRRTVESEGLDALVELKPLDGESRYRFST

YYQQKKKESG

>CYP547A1 AABX01000481.1 cont3.599 NCU09419.1(version3)

16175 MPPTGNPFNFARITLLALVEVLIVKKTSYRHVLAISTTSLVLAILAINYV

FFLAWKLYLYPVYFHPLSKFPAPKVVDLWRVLARFRGKVPPGQLLLELAE

RTPNDGIIILQGGFGTSMLITKPAPLADILVHHPYDFVKHDAIRNFLRPI

LGDGLVIVEGEQHRFLRKNTQPAFKFGHIKELYSTMWTKAIEMNHVLKEE

LREKGNDTSVEINAWAGKVTLDIIGIAAFGRDFHVLERPDHPLVKNYADL

LEPGPAKFAYFFLALTLSRKFVDLFPWEISRRFNRTTSNIRRICAELVRE

RKAAIEKLGDDQFDILSLLIRSNNFSEAELADQLLTFLTAG (2) 17197

17248 HETTSSAFTWAVYKLAQDGEMQSTLRSELKRALPDFPRFAPGQD

IAIILEHLPYLNGVMNETLRLYPTVPMTVRTATCDTTVLGHPIKKGTEIM

ISPWLINRYSDNWPSASTEFVPERWIDQEGKHIAEDGTEQPTQKPNNTGG

VTSNYAQMTFLHGPRSCIGQGFAKAELRCLLATFVLAFEWKLGMDEKDVM

PDGVITIKPNNGMHLILRSLDGHQKG* 17910

>CYP548A1 AABX01000409.1 cont3.400 NCU06895.1(version3)

71638 MVFGAAHLQLWLGALVVLGAVY (0) 71703

71769 TSCLIFYRLFLHPLAKYPGPFLAKLTDG

YMAYHAFKGDRHLEFWRMHEKYGKFVRFGPNSLSVNSNTALKDIYGFKAN

VRKAEFYDAFVHPAPNTHNARDRDLHARKRRVLAHAFSDSAIKEVERYIL

SNIRTFCDAIGDLGRPSFAPLADKKGWSSPKNMSDWCSWLAMDILGDLCF

GKAFHMLERPDNRYAVDLVSVAAHRHLICGTMPMLDKLSLDRIFLRKIAS

GRAQYMAYSRQQLAERTALGDETDRRDFFYYLLKARDPETGQGFTTPELW

GESNLLIIAGSDTTSTAMAATLFYLVRNPTALARVTAEIRSRFASVEGIH

QGPSLQSCTYLRACIDEAMRLSPSVGGLLPREVLPGGITIDGEHVPQGTI

IGVPHYTIHHNAAYYPDPWSYTPERWLSTRSPSEKTSGAAMQTEEQIQLA

QSAFCPFSIGPRGCIGKGLAYIEMSITLARVLFLYDLRRAVGVDDPAEGG

KKGAEYGRHRVGEMQLVDTFTSTKDGVMVEFRPREDLAA* 73322

>CYP549A1 AABX01000759.1 NCU01573.1 cont3.66(version3)

39539 MLIALLAPFSLLIAYVLHHVLSHQTLLLKQIFSQTIFNTIQLARLIGPLD

PYDPLTVPDQVIPDSNSASEISEAQARANQRIDDARSIIDFSYAGPKIEL

EDRLRLRALANSRLVAAFGINTSLTSSSVSVHKKFRKLASASINKSRADW

QKLYGVCMDFLQREKARYGRTGIGLAECVRCLCFVVVLVSQFGVDDKKVD

KNLVKRITDGINAQWLKSKEDGEAVKTSDSLNHDLGMLFGNLKTSVAEEN

GTGKDTSIDPSEALGLIMPQYETLWRVVLLTYVTAYYTQYDKRSLKKRVK

DIPSCLGNAATEKEALKLAT (0) 38580

38519 EGLRLYPSNNSIYRAATGPGPLKSADVQAC

HRDFNVWGRDALEFRPERFDNLTPLQEKAYFPFSLGSHKCPAFGGFGNRM

VTMLVVSMGRALSPETGKLNFEDAQLDNRVGIILPTGRDEMENWSWDMVR

V* 38124

>CYP550A1 AABX01000727.1 cont3.288 NCU05128.1(version3)

125984 MTVENSIVQHALTVYHDLSTATKATLAISIAFILYRLLFRHESTRRKELP

AWGPIEMGLVMYLLDGARNGIVYRVY (2) 125757

125699 SAIRRYGGSFYGISSANQTLIGYE

DVDRLFSSQMNHALSIEWHGYGLFLRFFGFPDTPTLKKKVESTFKPWHSP

IERVFNNDAGATAAFERGNLPKIVSEFVSFSQEKEKMKRWELRADIKVIK

QGEAVEANLSALIMDFGACFTIPPIFGHDLLDRNPHLIEDIWKFDHVVPL

LFINMPTWAPFKVMKEGIEARKRLLAGVEAFSRRVTQFQRGEKVDDGADI

SDVSDVVLERNTALERNEWSYQERAASELLVLFASNTNTQPVLFWLILYI

YSTPGLVNTLRQEVAPFITLSDSHHDKKGITAINTSGLSRECQLLKSIIL

ETLRLAFGAVSTRFVKRPITVQDGNHNHKLYPGTFLSVPHSFFQRDPSLY

PDPHKFVPDRFLETDPSSGKLVAKYGKLRPWGAGASMCRGRVFAEKELMA

IGAALVTLWDISPVGGGEWTVPEMVGGPGPVKPKEEVRVVIKRRVFGGVS

SSE* 124263

>CYP551A1 AABX01000129.1 cont3.541 NCU08716.1 (version3)

67094 MAVLNWASSRAATLSPCPRTAALVVITTWLLYAIGLAVYRLYFH

     PLARFPGRKMAAVTTWYEFYYDFWYGGKYIFEIEKMHKEF (1) 66843

66762 GPIVRINPHELSIHDPEYYNELYVGSSKRRTNFWPLFQGCTDDTD (1)66628

66552 VNHFMTIDHDLHRQRRKPFDPFFSRMAINIRYWPMLAEKASFLES

     RIRECKGQGKAVRLDRAFSAFSADCIERICTDDLEPGDGFLDQPD

      FGPEWYDGMLGLIRNAPILTKFPKALS (2)66202

66144 SLRYVPPRLLLWLFPQGRIANKYDARTRTQIHKAVTKQHLSKD

     TTTNSEHTTLFRALAQSDNLSPADKAEERLVREAKLIFLGGTIS

     TGRTLSFVSYYILSRPDIKARLEDELRDVMATWPEVVPTWTELE

      KLPYLQAVIKEALR (2) 65710

65588 LSYGFMRRLPRVSPDVAIQYKEYTIPPGTPVGMSAYLMHSNPEV

     YQDPDQFVPERWLGGDTRVMQRSYVPFTKGSRSCLGQN (2) 65343

65254 LSMAEISLVLAVLFRPDGPRMELFETDESDVKHVHDFVVPLPK

      LDSLGVRVMVR* 65090

>CYP552 AABX01000480.1 cont3.625 missing about 32 aa

    at N-term (GC boundary at exon 2?)

   2FFHPLRHIPGPKLAAASYIPEFYWDVLRGGMYQKRILLMHEKY (1) 130

 229GPVVRISPNEVHCNDWRFIDEIYASGSRKRDKPLHQVRGSGG (2) 354

 419IVDATFSTTSHDLHRMRRNALNKFFSRAQVARLEPTVRENAERIC

    DKILILGKSAPFDVTTAYSHFTTDVISGYCLGDSLGLIEQKGWEP

    NFREPLYAQLKLMYWLRFIPLFKRLS (0) 766

 888 WYVFFGESFLPCPVSLTNYDIQGDMPRYVRRAQDNVIKGLDDG

    SSVFGALLTSDLPPSEKSLERLTDEGFSLFAAGTETVSWALSVIT

    YHLFANRELLEKVTKEVTQVIDAKSGQLPSWHTLEKLPYLGGVIY

    EGLRLSYGVASRSSRIPVGEDLVYRG (0) 1364

1407 YVIPRGYAIGMSPLSIHHDETVFPNSHSFIPERWIDDNDQHRKEL

    DRALLSFSKGSRSCLGIN (2) 1595

1657 LAYCELYVLLSLLIVRVFPHMKLHETTEVDVTHDHDFFNPFPVSS

    SKGVRAIVV* 1821

>compare CYP552A1 toAACD01000032.1 Aspergillus nidulans

MTISAETVLRLLPLTAGGAWVFFHLAKCIYNL

FFHPLSHIPGPW

LAAATYLPEFYHDVVRGGRYTTQIQQMHEKYGPIVRINPHELHCNDHRFINEIYAGGT

RKRDKPVHQ

VTGSEAAAH ATFSTTDHDVHRMRRNALAKFFSRAQVLRLEPTVRGLAER

LCDKMLRTEKGRPFHVGAAYSQFTADVISGYCLGENLGLMGQEGWE EQIVDEVATVLR

LGCTLRFVPWWKHVLGVVLLIRKQLVGSPKESSDALIVGMPNHIRKAQADIKIMDGKG

NGNGPETIP

TTVFTSLLQSDLPPQEKTFERMTSEGISLFAAGTATVSWALTVATYHLL

TKPAILQRLTAEVSDLVNSSSCTEKVEWSALERLPYLGAVIQEALRLSYGVASRTARV

ATGEDLVYRGEWAHREVQYIIPRGSAIGMSAVLAHHDEAVFPDSHLFLPERCSLSARA

EVEVRVGFNFANGCSLAYCELYLLLALLVVRVFPRMRLYGTTEADVAYDHDFFNPFPV

WESKGVRVVV

>CYP553A1 AABX01000379.1 cont3.619 NCU09565.1(version3)

thisN-term may be too long

26616MPSTMADAVAEIESLLDTGIAAIKGLIP

     MIWVQSKTSPVQFLVGGVAVLLSYLLLTISPPHDPSEPPLIKPR

     FPVIGHLFGLLNHQVNYFSELFARYHLPIATLPIGNQKLYVIFD

     APLQQAALKAKDMDAQSFMVDFVPRIFGVKQGTVDKLLGKDGVH

     PNIMGDMEQVFKSALSGDNLQKLASTTLATMADTLNDVDSKKGT

      KIPNMFLWLQSLLSRSTSKALWGEKHNPFKDHQVIDAQW(2) 25888

25827 DFESHLGPLVLGVLPSIVARRAYLARNKVQSALLSYFSARHYET

     DPSTSYFIRARTRLLKRYDLPDDELAKNEVAITLVATTNVLSTL

     FWCIAEIWTRPDLLQQIRAEALLAVFGNSQKALSGEDMGTRTIT

     IPATGLDAKCPLLASCFRESIRLASQIVTARRVLKDTLLTDPVS

      GNSYLLKADTNVMMPAKVVHRNTSVWGADAEGFNPRRFMWEELG

     PKVERQRKAAFVPFGGGKHMCPGRHFAFTENLALMAALAIGFEI

     EGLDRSKLKMGDSKRGETAKPLPGLEGGEVVLKKRKGWEGVEWEFSC* 24892

>CYP526B1 AABX01000480.1 cont3.625 NCU09636.1(version3)

38% to 526A1 100% match to XM_330285.1 mRNA prediction

5049 MLSVVQQSIPFFDNIFNLAKPLLVLLTVTVTLYSLYRWLLPKPIPGIPYN

PQAAKSLLGDAPAMIKEVSATGEFRVWCAKQMRQMNSPICQIFIRPFSKP

WVLLADFREAKDILMRRKEFDKSSFLSDGMACMGSFHGIYMTGEKFKANR

QLIQDLMTTSFLNNHVGPAIFGKGLELMKLFETKMLLARGRPFSVKKDFE

YTSLDVMLEFAFGKNWVHTSLGPQMELVGKMQAEDIDAMMEGKDSDEPVV

FPLVPLVDFLNSVYEAPEIVEKTINALMPKLQTWWWSKQSWYKHIFDAKE

RAMKEQVEIAISNFQSGCIQTGIEHMLMREASRAEKECRAPDFRSKVLRDE (0)6101

6163 MFGNIVGGHHTTSGAMMWLTKYLTDHPVVQSNLRSVLYTTLSAAKNENR

LFTFEEIRHAKLPYLDAIIEEMLRINAVPVTREALTDTTILGYPIKKGTQ

VFFMSNGPGFLSPSFPIDESRRSETSRASKINATWDETQDLAAFVPERWL

VRKREGNGLLADDVDFDGAAGPQLVFGLGPRTCWGRRLAYMEMRIVISML

VWNFELLKTPPALSSYAGLEGIARVPQQCHVRLRKL* 6870

>compare CYP526B1 aboveto AACM01000167 Gibberella zeae

MSSHLVDLYEQAPKSFILTLFLSIASTLYLVRRALLPKPIPGIP

YNKNAINSIMGDMAEFRAAKNRREWWALQAVKHQSPLVQVFMKPFGLPWVFVADHFEA

SDICMRRLKEFDRADITRQQFDGLTPGHQITLKTSDPQFKKNRELVRDLMSSSFLQQA

TAPQIHHKFSTLMSLWDRKRDLSGGRPFDISHDIHNAAFDIILGASFGVDSGQGQIGK

ELDELRSKTTTGGNDDAFEFERVPFYDEELGCFDTLIESFNVSIRSPYPLFSHFLYRN

LSPKMREANAMRAKLQDREIAKSIERREKGHPQRCALDNMMAREDAIAEKEGRKPDYR

SQAILSELLGYLVAGHETTSGVLRWGMKYLTKDQRVQSILREAFRTGYADALKQSRVP

TMDEILKAHIPYLDAVIEEMLRHARVAPITLREAVEDTQILGHHIPKGTTIGFLGNGP

GVMMPSIPVDPNKRSEASQAHVNKMELFDESNISQFVPERWLTTQTNEKGEEVTVFDH

NNGPQQAFGLGPRACFGKKLAYMEIKAFFTFVFWEFKLEPVKAELATDEEMVALTRAP

KNVFVKLTKVECCG

>CYP559A1 AABX01000439.1 cont3.339 NCU05967.1(version3)

29% to 54A1 100% match to XM_325821.1 mRNA prediction

53814 MAITSLSSHLLESLLASLTLGRVTALVILFTIASFAIDSTRKPTYPKSLP

RVGFTGGIGATVRNWMNLGNYHNWVEEGYNKYGKHGRSFVTPSSPSQPDE

IVVPQEQIAWMLEQPETVASTQESHKDVLFGTYNFFNVDDHFPTKVMHKH

LARNLVTLLPDIQQEVAESIDIAFGTDTENWTTLNLWEALLAIVPRVMNR

LAVGLPLCRNQAFLSSQVGFADDVFRNCLILTLFPTVLHPLVGRLVTLPN

WYHFHQGYRLLKPVIEQRLRDYERFESGESQTPPEESLITWLIRQAKADN

LPHEQTPYMIAMRLLPIEFAAIHTTVITLHALFLDLLASDPSKDYLSILR

EETTAVLQSEPNGIWTKNGLAQLYRTDSAIRESMRLSTFSTGLTHRKVVA

KEGITNPFEGWHAPYGAILMANMSVQHDADIYEDPKTYDALRFSRVREEY

EARSQEEVEKGKETEEALRIKRLGVVTTSSEFLPFSHGRHAC (2) 55289

55352 PGRFFVAHEVKMMLAHILQNYDIKPIAERPRPTWVAHTIIPALD

      VKIEVKRRQRKK* 55532

>CYP560A1 AABX01000445.1 cont3.580 NCU09274.1 32% to CYP503

XM_331965.1 = 100% match mRNA prediction

22936 MNTLHNISSPPGPLDPYANPFHLAIKDRIEHLSYPKTFALILITSWFLST

TAKWLTNGKRKKLAIPTWGYRSWFEPTLLLQARFVMGARQLVHEAYTK (0)23229

23290MPNMPFVLKRYDADFLILPIRYLDELRLIPLSKLSNKGANVA (0) 23415

23484 NLVPEYHGIT

FLLTNNLHVEVLRKKLTPELWKFIDLASTELSHGWDISLPHSQLSANGND

WVKIDFERTVRDLVSRMTAKVFLGNPACRDETWLGISVAFSIDLFTASFT

MKMFPPWMYPVVAPLIPARRRVLKQLKAARGIVGEQMRKHASLKEKRAQA

ARGEVSLTAEEAQDTLLNWMLDHGKGDEVRVEEMSARQCVLTLASIHTTA

SQAANMMFDLCEHPQWWGVLQEEVDEIAKELGPPGLAGGMKEGGGGVSVK

EWCVRLEKLDSFFQETQRREPPIM(1) 24335

24430 LGPQRLAQQDVTLKDGTFIPRGTRVA

FPMLEHQMDPQTYENPNEYDPMRFYRKRHESPDQMHLHMAGQTHPNVLGF

GYGNQACPGRQFAVAEIKLIMARMLYEFEFKFADEKKGRPKTGYINEMAM

TDWNARLLMRKRVR* 24852