A search of the Neurospora crassa genome has identified 38 P450s. most are now full length. All Neurospora P450s have bee named, most from 527A1 to 553A1, but there are sme that were named already or fall in existing families. CYP51, 53A4, 54, 55A6, 61A5, 65B1, 65C1, 68D1, 505A2, 507A1, See the lower eukaryote part… Read More
The fungus Phanerochaete chrysosporium has been sequenced. The P450s from this Basidiomycete have been searched and the preliminary results are on the homepage (see the main table). The big surprise from this work is the large number of P450s found. At least 122 signature sequence regions are present and 167 P450 fragments are found. These… Read More
I have added Xenopus P450s to the animal part of the homepage table. This is now pretty complete for current data. I will be adding bovine and pufferfish sections based on ESTs and GSS (genome survey sequences).
CYP26C1 is complete. CYP27C1 is complete. There is a new CYP4A22 sequence that is 95% identical to 4A11. Three genomic sequences agree, but all ESTs are like the 4A11 sequence and not like this new genomic sequence. Therefore, there seem to be two different genes, 4A11 and the new closely related 4A22 sequence. The human… Read More
A complete set of public mouse P450 protein sequences is posted on the homepage under the table and other info. It is also reachable under the mouse button. 75 mouse P450s are given. 5 confidential sequences are named but not given. 6 human sequences with no mouse orthologs are also given in anticipation that they… Read More
42 new P450s from Dictyostelium discoideum have been named. The sequences and accession numbers or other identifiers from the Dicty Blast server or the Kazusa cDNA project are give in the lower eukaryotes section of the web page. 705 sequence fragments have been identified and sorted into 55 contigs. There are 26 full length sequences.… Read More
The Dictyostelium sequences have been revisited and many new accessions have been found. Jinchuan Xing a graduate student on a rotation in the lab has searched all 64 previous contigs against the databases and he assembled as many of these genes as possible from the new hits. There are now 17 complete or nearly complete… Read More
A new Human P450 has been found and it has orthologs in mouse and bovine and fish. It may be vertebrate specific since it is not found in fly or worm. The new sequences are all being named CYP20 (a long reserved and unused animal p450 name) Human CYP20 (phase of introns shown) AC011737.8|AC011737 Homo… Read More
The assembled human genome is due for publication on Feb 12 in Science (online access free for whole genome issue) Hard copy publication on Friday Feb. 16. I will be searching for the missing regions of 27C1 and 26C1 when this comes out. Other notable findings are a complete diatom P450 (sequence still confidential) that… Read More
The Arabidopsis genome has seven unsequenced clones and one 5kb gap not including the centromere gaps. There may be a P450 on one of these clones, since CYP84A4 has not been found yet. There are also three GSS sequences that do not match known P450s. To see the details click here
