Two new sequence alignments of the I-helix to the end half of the proteins are posted. The first alignment covers 73 sequences (mainly insect sequences) in a 4 family alignment. The second alignment has 79 sequences (mainly 6, 9, 12, 28 families) with many new Drosophila sequences included in both alignments. See second alignment. These… Read More
The Arizona P450 site is now linked. Please go visit this excellent site. The P450 Site at the University of Arizona
There are now 69 N-terminal sequences in an alignment under the Drosophila button on the main page. That means there are at least that many P450s in Drosophila and probably a few more will be found, since C. elegans had 80 P450s. The FASTA file of P450 sequences has all the fragments I have so… Read More
Celera genomics continues to deposit new Drosophila sequences into Genbank. On Nov. 16, 1612 fragments were deposited. I am trying to catch up to these. See the N-terminal page for an alignment of 65 Drosophila N-terminal sequences. The drosophila list is also being updated. See the accession numbers beginning with AC0#####* followed by an asterisk.… Read More
Celera Genomics deposited 551 Drosophila sequences into Genbank on Nov. 3, 1999. These represent about 10 million bases of sequence. The remaining Drosophila genome sequences from Celera should be in Genbank by the end of the year. This will allow identifcation of all the P450s in a macroscopic animal.
The Dictyostelium discoideum genome project has been making progress. In Nature 401 from 30 Sept 99 page 440 the current status is given as “Over two-fold coverage of the 34 Mb genome is now available and there is already information on at least 90% of the genes.” I have searched the Jena web Blast server… Read More
A new human CYP family CYP39A is now represented by a complete sequence. This was assembled over several days by using every trick in the book to find all the pieces. I suspect this will be an important P450, with an ancient history, probably acting on sterols. The gene is also found as ESTs in… Read More
A new human P450 has been found named CYP26B1 that is 44% identical to 26A1 from mouse and humans. The sequence was found in genomic DNA AC007002. The reference is in press. Nelson, D.R. (1999) A second CYP26 P450 in humans and zebrafish: CYP26B1. Archives of Biochemistry and Biophysics in press.
The server has been upgraded from an ancient Quadra 650 circa 1994 to a PowerMac 7100. This is not new, but it is a step up from the old Quadra. You should notice some improvements in speed. If you have any trouble accessing any part of this site let me know so I can check… Read More
A new tree of the CYP81 family is posted under the trees button. This does not contain all CYP81 sequences, but it has all the subfamilies represented.