A press release of July 28 from Celera stated that one million sequences (500 million bp of sequence ) have been completed from the Drosophila genome. Below is a quote from the press release. “Celera expects to complete the random sequencing phase of Drosophila in early September when it will begin sequencing the human genome.… Read More
Celera Genomics will finish the Drosophila genome in August or September according to an NPR interview with Craig Venter that aired on July 27. It was not mentioned when the sequence data will be posted to Genbank. The rice P450 page has been updated. There are some new ESTs and some contigs have been joined
A tree with 30 plant P450s coving the CYP93, 705, 706 and 712 families has been posted under the trees button on the home page. The CYP93D1 and CYP712A2 sequences had their names reversed in the bibliographic pages and FASTA files. This has been corrected.
The only possible mitochondrial P450 from C.elegans has been reexamined to better identify the intron exon boundaries based on similarity to CYP12A sequences of insects. One previously missed exon is added based on an EST sequence and another exon is extended to fill a critical gap. Only about 40% of the sequence is covered by… Read More
Rice P450s have been updated by exhaustively searching the genbank entries for new accession numbers. The sequences have been translated and added to the FASTA P450 rice list. See the rice button on the homepage for more info. There are 192 accession numbers for rice P450s
Two new trees have been made with CYP4 sequences as the main content. These trees illustrate the difficulty of maintaining a sensible nomenclature when families get very large. There are some inconsistencies. The 4D subfamily has been split over time into two different clusters of sequences. Cyp4p should probably be in a separate family. The… Read More
A Tree of 56 Insect P450s The tree with 56 insect P450s includes many new Drosophila sequences. Some are not yet named. This tree is based on an alignment that covers the I- helix to the ends of the sequences, since many are missing the N-terminal. The 4 family sequences are not included here. There… Read More
The Drosophila P450s have been found in Genbank by systematic BLAST searches of the nr, month, others ESTs, gss and htgs sections, using different P450 family representatives. The first search with Cyp4d2 yielded 101 new ESTs, 6 new sequences from month, one from htgs and none from gss or nr. The second search with Cyp6d2… Read More
The Drosophila P450 FASTA sequence file has been updated by including many new sequences starting with genbank numbers AI (ESTs) and AL (genome survey sequences) Work continues to sort these and assign them to known sequences or related families.
I have done a preliminary search of the new Drosophila sequences and havent translated many of them in the file Drosophila P450s. .I am also finding some older P450s that have come out since I last worked on the Drosophila sequences.
