The Arabidopsis genome has seven unsequenced clones and one 5kb gap not including the centromere gaps. There may be a P450 on one of these clones, since CYP84A4 has not been found yet. There are also three GSS sequences that do not match known P450s. To see the details click here
The Genome of Arabidopsis is complete and described in Nature and Plant Physiology today. Over the course of the day I will be searching for the missing P450 genes that are currently represented as fragments and I will be incorporating them into the website. This depends on the availability of the sequence for searching. Sometimes… Read More
I have begun a new long term project to detail the molecular events that have shaped the evolution of eukaryotic life. I have a great interest in this area and I would like to start this as an open ended and ever growing review/ text on the specific innovations and dichotomies that make up eukaryotic… Read More
The number of mapped clones in Arabidopsis that are not in Genbank has dropped to 31. A new Arabidopsis P450 CYP86B2 has been found on Chr V. Science has delayed it annual Genome issue for several weeks. The latest previous Genome issue was Oct. 25. What are they waiting for? Is it Arabidopsis? Is it… Read More
A new seach for human P450s in the human genome project data has been done. The files listing human P450s by accession number, chromosome or name will be updated. A new human P450 was found named CYP2W1. This sequence may have a frameshift at the C-terminal, since another frame has an exact match to the… Read More
This year a large number of Arabidopsis ESTs have been deposited from Japan. On April 20, 2000 3366 ESTs were deposited and on June 27, 2000 49848 more ESTs were deposited for a total of 53233. These EST accession numbers start with AV. A search of these ESTs for P450s identified 305 as P450s (0.6%)… Read More
All 90 Drosophila sequences on my website have been blast searched vs the sequences on Rene Feyereisen’s web site and all the differences between our translations have been identified. All these differences have been checked against the Genbank entries for the Drosophila genome. All differences have been resolved and corrections made to my site. This… Read More
Rice P450s have been thoroughly updated. 358 accession numbers and 213 contigs including 40 complete rice P450 sequences are now available. Go to the rice button on the home page. Read the rice P450 report.
All 269 genes from Arabidopsis have been listed by chromosome in the order that the clones appear on the chromosomes. This is a linear map without the actual spacing that would be included in a real map. See Rene’s site for the drawn maps, though that site does have some errors and many new genes… Read More
The Arabidopsis sequencing totals web site showed a dramatic jump yesterday From 92.9% complete to 103.7% complete. TOTAL The number of bases done is now 134,819kb which is a little larger than the predicted size of 130 MB. I think this means that the Arabidopsis genome is done or almost done. When I searched Entrez… Read More
