The Drosophila P450s have been found in Genbank by systematic BLAST searches of the nr, month, others ESTs, gss and htgs sections, using different P450 family representatives. The first search with Cyp4d2 yielded 101 new ESTs, 6 new sequences from month, one from htgs and none from gss or nr. The second search with Cyp6d2… Read More
The Drosophila P450 FASTA sequence file has been updated by including many new sequences starting with genbank numbers AI (ESTs) and AL (genome survey sequences) Work continues to sort these and assign them to known sequences or related families.
I have done a preliminary search of the new Drosophila sequences and havent translated many of them in the file Drosophila P450s. .I am also finding some older P450s that have come out since I last worked on the Drosophila sequences.
On May 28, 1999 28,049 Drosophila genome survey sequences were deposited from Genoscope in France. These are BAC end sequences. The percent of the Drosophila genome sequenced as reported at the MOT tables jumped from 15% to 24%. I have not had a chance to search these for P450 hits, but there should be a… Read More
NCBI has started a new service called Locus Link. This is an attempt to collect information about a family of proteins/ genes on a single page with links to the relevant databases like OMIM, Genbank, Unigene etc. There is a cytochrome P450 page. Some of the information comes from the Cytochrome P450 homepage, which is… Read More
Having trouble with those pesky human P450 polymorphisms? Try The Official Human P450 Allele Nomenclature Site (in Sweden).
A possible chloroplast P450 has been reported from almond this sequence is probably in the CYP71D subfamily. Therefore, the whole 71D subfamily might be localized in the chloroplast. Chloroplast P450
A large cluster of Arabidopsis P450s has been found on AB024038. This sequence contains 14 different P450s and only two were known previously. See CYP86C2 and CYP71B16 to CYP71B26. CYP71B3 and CYP71B4 were already known.
A discussion of early eukaryotic P450 evolution is available looking at P450s in fungi and the slime mold Dictyostelium. Read Me
The CYP72 family has been sequenced in Arabidopsis (accession number AB023038) and there are 8 genes and one pseudogene present. This is similar in size to the Arabidopsis Z97338 gene cluster of CYP702 and 705 genes that has 8 genes and two pseudogenes. The spacing between the genes is less than 1000bp except for one… Read More