The accession numbers AC008537 and AC008962 from human contain a large cluster of P450 genes from Chromosome 19. This cluster has been described in Hoffman et al. J. Molec. Evol. 41, 894-900 1996. However, the sequence of the cluster is now coming into the databases on these two accession numbers. These are incomplete sequences at… Read More
All Drosophila melanogaster P450 protein sequences have been posted as a FASTA File All Drosophila melanogaster P450s These sequences are all in Genbank. There are no confidential sequences left. There are 86 P450 genes and 4 pseudogenes. CYP51 is absent. Since CYP51 is also absent in C. elegans, this important eukaryotic sterol biosynthetic gene may… Read More
I have posted a 4 family tree with 89 sequences, including the new Drosophila sequences in the 4 family and in the 18 clan. New 4 Family tree A second tree covering the remaining sequences including the 6, 9, 12 and 28 families is also here New 6 and 9 Family tree
Two new sequence alignments of the I-helix to the end half of the proteins are posted. The first alignment covers 73 sequences (mainly insect sequences) in a 4 family alignment. The second alignment has 79 sequences (mainly 6, 9, 12, 28 families) with many new Drosophila sequences included in both alignments. See second alignment. These… Read More
The Arizona P450 site is now linked. Please go visit this excellent site. The P450 Site at the University of Arizona
There are now 69 N-terminal sequences in an alignment under the Drosophila button on the main page. That means there are at least that many P450s in Drosophila and probably a few more will be found, since C. elegans had 80 P450s. The FASTA file of P450 sequences has all the fragments I have so… Read More
Celera genomics continues to deposit new Drosophila sequences into Genbank. On Nov. 16, 1612 fragments were deposited. I am trying to catch up to these. See the N-terminal page for an alignment of 65 Drosophila N-terminal sequences. The drosophila list is also being updated. See the accession numbers beginning with AC0#####* followed by an asterisk.… Read More
Celera Genomics deposited 551 Drosophila sequences into Genbank on Nov. 3, 1999. These represent about 10 million bases of sequence. The remaining Drosophila genome sequences from Celera should be in Genbank by the end of the year. This will allow identifcation of all the P450s in a macroscopic animal.
The Dictyostelium discoideum genome project has been making progress. In Nature 401 from 30 Sept 99 page 440 the current status is given as “Over two-fold coverage of the 34 Mb genome is now available and there is already information on at least 90% of the genes.” I have searched the Jena web Blast server… Read More
A new human CYP family CYP39A is now represented by a complete sequence. This was assembled over several days by using every trick in the book to find all the pieces. I suspect this will be an important P450, with an ancient history, probably acting on sterols. The gene is also found as ESTs in… Read More
